LOCUS       DAXFZR010000001        59412 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000001 DAXFZR010000000
VERSION     DAXFZR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 59412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59412
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.001"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(300..1235)
                     /gene="hemC"
                     /locus_tag="ACFJAS_000001"
     CDS             complement(300..1235)
                     /gene="hemC"
                     /locus_tag="ACFJAS_000001"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC3981381.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1354..1869
                     /gene="ybeY"
                     /locus_tag="ACFJAS_000002"
     CDS             1354..1869
                     /gene="ybeY"
                     /locus_tag="ACFJAS_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC3981382.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYLRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1871..2695
                     /locus_tag="ACFJAS_000003"
     CDS             1871..2695
                     /locus_tag="ACFJAS_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC3981383.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2838..4217)
                     /gene="nhaC"
                     /locus_tag="ACFJAS_000004"
     CDS             complement(2838..4217)
                     /gene="nhaC"
                     /locus_tag="ACFJAS_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC3981384.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATVGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4507..5730)
                     /locus_tag="ACFJAS_000005"
     CDS             complement(4507..5730)
                     /locus_tag="ACFJAS_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC3981385.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            6166..6579
                     /locus_tag="ACFJAS_000006"
     CDS             6166..6579
                     /locus_tag="ACFJAS_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC3981386.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6626..7255
                     /gene="nth"
                     /locus_tag="ACFJAS_000007"
     CDS             6626..7255
                     /gene="nth"
                     /locus_tag="ACFJAS_000007"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC3981387.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7393..8892
                     /locus_tag="ACFJAS_000008"
     CDS             7393..8892
                     /locus_tag="ACFJAS_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC3981388.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            9095..10615
                     /locus_tag="ACFJAS_000009"
     CDS             9095..10615
                     /locus_tag="ACFJAS_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC3981389.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRADV"
     gene            10672..11757
                     /gene="nagZ"
                     /locus_tag="ACFJAS_000010"
     CDS             10672..11757
                     /gene="nagZ"
                     /locus_tag="ACFJAS_000010"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC3981390.1"
                     /translation="MTVPHIPRGPVMAGIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGIKERAR
                     ISFEAGCDIILVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11838..11914
                     /locus_tag="ACFJAS_000011"
     tRNA            11838..11914
                     /locus_tag="ACFJAS_000011"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11872..11874,aa:Pro,seq:cgg)
     gene            complement(12250..12888)
                     /locus_tag="ACFJAS_000012"
     CDS             complement(12250..12888)
                     /locus_tag="ACFJAS_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC3981391.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLKPAPPQIDYVATSPKACYAAKYETNCIAEADEVRIVADGDVLAFCENMERK
                     IRARHYYLSPCEQNGVMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(12885..13253)
                     /locus_tag="ACFJAS_000013"
     CDS             complement(12885..13253)
                     /locus_tag="ACFJAS_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC3981392.1"
                     /translation="MKFPSTLLVLLVAAEHFYIAWLEMTQIPGEKAAEMFKLPYEFME
                     QKRVQTLFGNQGLYNGFLAVGLLWAQFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
     gene            complement(13519..13941)
                     /gene="queD"
                     /locus_tag="ACFJAS_000014"
     CDS             complement(13519..13941)
                     /gene="queD"
                     /locus_tag="ACFJAS_000014"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC3981393.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYGRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(14029..14544)
                     /locus_tag="ACFJAS_000015"
     CDS             complement(14029..14544)
                     /locus_tag="ACFJAS_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC3981394.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(14572..15231)
                     /gene="queC"
                     /locus_tag="ACFJAS_000016"
     CDS             complement(14572..15231)
                     /gene="queC"
                     /locus_tag="ACFJAS_000016"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC3981395.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVKQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(15416..15621)
                     /locus_tag="ACFJAS_000017"
                     /pseudo
     CDS             complement(15416..15621)
                     /locus_tag="ACFJAS_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            15720..16946
                     /locus_tag="ACFJAS_000018"
     CDS             15720..16946
                     /locus_tag="ACFJAS_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC3981396.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEGRVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAMPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGRTLSVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDTVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(16967..17248)
                     /locus_tag="ACFJAS_000019"
     CDS             complement(16967..17248)
                     /locus_tag="ACFJAS_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3981397.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            17327..17443
                     /locus_tag="ACFJAS_000020"
                     /pseudo
     CDS             17327..17443
                     /locus_tag="ACFJAS_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17444..19162)
                     /locus_tag="ACFJAS_000021"
                     /pseudo
     CDS             complement(17444..19162)
                     /locus_tag="ACFJAS_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(19264..19479)
                     /locus_tag="ACFJAS_000022"
     CDS             complement(19264..19479)
                     /locus_tag="ACFJAS_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981398.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(19493..19840)
                     /locus_tag="ACFJAS_000023"
     CDS             complement(19493..19840)
                     /locus_tag="ACFJAS_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981399.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(20025..21299)
                     /locus_tag="ACFJAS_000024"
     CDS             complement(20025..21299)
                     /locus_tag="ACFJAS_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC3981400.1"
                     /translation="MNRPKQPFFRPEVAIARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQETMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLLLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(21360..21632)
                     /locus_tag="ACFJAS_000025"
     CDS             complement(21360..21632)
                     /locus_tag="ACFJAS_000025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981401.1"
                     /translation="MLPQPKEVPHGGILLQYLLLQRQFISCRKIFSVLYGANGVYNLT
                     RDWVNDAVNAPPYNGRPIFEIEHGLTAPATKADKSGNGYTDGTDYC"
     gene            complement(21707..21934)
                     /locus_tag="ACFJAS_000026"
     CDS             complement(21707..21934)
                     /locus_tag="ACFJAS_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981402.1"
                     /translation="MKNWKQFTSFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(21943..23097)
                     /locus_tag="ACFJAS_000027"
     CDS             complement(21943..23097)
                     /locus_tag="ACFJAS_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC3981403.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
     gene            23349..23630
                     /locus_tag="ACFJAS_000028"
     CDS             23349..23630
                     /locus_tag="ACFJAS_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981404.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            23627..24670
                     /gene="argC"
                     /locus_tag="ACFJAS_000029"
     CDS             23627..24670
                     /gene="argC"
                     /locus_tag="ACFJAS_000029"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC3981405.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
     gene            complement(25264..27306)
                     /gene="recG"
                     /locus_tag="ACFJAS_000030"
     CDS             complement(25264..27306)
                     /gene="recG"
                     /locus_tag="ACFJAS_000030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC3981406.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAGESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(27392..27835)
                     /gene="secB"
                     /locus_tag="ACFJAS_000031"
     CDS             complement(27392..27835)
                     /gene="secB"
                     /locus_tag="ACFJAS_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC3981407.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(27856..28113)
                     /gene="grxC"
                     /locus_tag="ACFJAS_000032"
     CDS             complement(27856..28113)
                     /gene="grxC"
                     /locus_tag="ACFJAS_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC3981408.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            28332..29825
                     /gene="rng"
                     /locus_tag="ACFJAS_000033"
     CDS             28332..29825
                     /gene="rng"
                     /locus_tag="ACFJAS_000033"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC3981409.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(29860..31111)
                     /locus_tag="ACFJAS_000034"
                     /pseudo
     CDS             complement(29860..31111)
                     /locus_tag="ACFJAS_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(31193..31867)
                     /locus_tag="ACFJAS_000035"
     CDS             complement(31193..31867)
                     /locus_tag="ACFJAS_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFC3981410.1"
                     /translation="MRVLLVEDDAMIAEAVSASLKDGGYAVDWVKNGAQVAAAAAAQP
                     YDLMLLDLGLPGRDGLDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKP
                     FDMAEFKARMRAVLRRGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNKEFSVL
                     QALLARPGVILSRSDSEDKVYGWGGEVESNAVDFLIHGLCKKLGKESIQNVRGVGWLM
                     PRQDAV"
     gene            complement(32090..33403)
                     /locus_tag="ACFJAS_000036"
     CDS             complement(32090..33403)
                     /locus_tag="ACFJAS_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC3981411.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILVSSIAAGIVPLP"
     gene            complement(33772..34344)
                     /locus_tag="ACFJAS_000037"
     CDS             complement(33772..34344)
                     /locus_tag="ACFJAS_000037"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC3981412.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPEVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            34503..35912
                     /gene="dacB"
                     /locus_tag="ACFJAS_000038"
     CDS             34503..35912
                     /gene="dacB"
                     /locus_tag="ACFJAS_000038"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC3981413.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(35977..37146)
                     /gene="metK"
                     /locus_tag="ACFJAS_000039"
     CDS             complement(35977..37146)
                     /gene="metK"
                     /locus_tag="ACFJAS_000039"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC3981414.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSEPGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(37329..37439)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            37492..38385
                     /locus_tag="ACFJAS_000040"
     CDS             37492..38385
                     /locus_tag="ACFJAS_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC3981415.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(38430..39494)
                     /gene="tsaD"
                     /locus_tag="ACFJAS_000041"
     CDS             complement(38430..39494)
                     /gene="tsaD"
                     /locus_tag="ACFJAS_000041"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC3981416.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEGKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(39610..40797)
                     /gene="ccsB"
                     /locus_tag="ACFJAS_000042"
     CDS             complement(39610..40797)
                     /gene="ccsB"
                     /locus_tag="ACFJAS_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC3981417.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATVLGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(40790..42742)
                     /locus_tag="ACFJAS_000043"
     CDS             complement(40790..42742)
                     /locus_tag="ACFJAS_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC3981418.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(43020..43643)
                     /locus_tag="ACFJAS_000044"
     CDS             complement(43020..43643)
                     /locus_tag="ACFJAS_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC3981419.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            43850..44479
                     /gene="yihA"
                     /locus_tag="ACFJAS_000045"
     CDS             43850..44479
                     /gene="yihA"
                     /locus_tag="ACFJAS_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC3981420.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(44599..46995)
                     /locus_tag="ACFJAS_000046"
     CDS             complement(44599..46995)
                     /locus_tag="ACFJAS_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC3981421.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            47147..48262
                     /gene="pilM"
                     /locus_tag="ACFJAS_000047"
     CDS             47147..48262
                     /gene="pilM"
                     /locus_tag="ACFJAS_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC3981422.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            48265..48864
                     /locus_tag="ACFJAS_000048"
     CDS             48265..48864
                     /locus_tag="ACFJAS_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC3981423.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            48865..49512
                     /locus_tag="ACFJAS_000049"
     CDS             48865..49512
                     /locus_tag="ACFJAS_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC3981424.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            49530..50075
                     /locus_tag="ACFJAS_000050"
     CDS             49530..50075
                     /locus_tag="ACFJAS_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC3981425.1"
                     /translation="MKHYALLISFLALSACSQNSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            50094..52289
                     /gene="pilQ"
                     /locus_tag="ACFJAS_000051"
     CDS             50094..52289
                     /gene="pilQ"
                     /locus_tag="ACFJAS_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC3981426.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVSGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQ
                     AEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
                     TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLK
                     NETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESL
                     SKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGSNSTNTELKKAVLGLTVTPNI
                     TPDGQIIMTVKINKDSPAQCASGNNTILCISTKSLNTQAMVENGGTLIVGGIYEENNG
                     NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
     gene            53557..54069
                     /locus_tag="ACFJAS_000052"
     CDS             53557..54069
                     /locus_tag="ACFJAS_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC3981427.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            54149..55228
                     /gene="aroB"
                     /locus_tag="ACFJAS_000053"
     CDS             54149..55228
                     /gene="aroB"
                     /locus_tag="ACFJAS_000053"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC3981428.1"
                     /translation="MKTLTVHTPSHSYPIFVGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
     gene            55967..56809
                     /locus_tag="ACFJAS_000054"
     CDS             55967..56809
                     /locus_tag="ACFJAS_000054"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC3981429.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            56838..57302
                     /locus_tag="ACFJAS_000055"
     CDS             56838..57302
                     /locus_tag="ACFJAS_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC3981430.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            57334..58443
                     /gene="ribD"
                     /locus_tag="ACFJAS_000056"
     CDS             57334..58443
                     /gene="ribD"
                     /locus_tag="ACFJAS_000056"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC3981431.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGIGSGLFCLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNGNAF"
     gene            58597..>59412
                     /locus_tag="ACFJAS_000057"
     CDS             58597..>59412
                     /locus_tag="ACFJAS_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC3981432.1"
                     /translation="MDTKEILGYAAGSIGCAILAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLAMSVLCLGLDQAYIREYNAAADKDILFKALFLPPLLSAAAIAAFLLSHPALPSE
                     ILFSLDDAAAGIGLVLFGLSLLPIRFLLLVLRMEGRALAFSSAQLVPKSALLLLLLTV
                     WLLHFPARTAVLTSVYALANLAAAAFLLFQNRCRLKAVWRAPFSPAVLHRGLRYGIPL
                     ALDSLAYWGLTSADRLFLKKYAGLEQLGIYSMGISFGGAALLFQSIFSTVWTPY"
BASE COUNT        14628 a        16948 c        14592 g        13244 t
ORIGIN      
        1 ggtgtggaat ttagggataa tctagtacag ccacaataaa aaatacgaat atcatcattc
       61 tcaaattcaa aatttgaacg ccacagtatt atccgaagcc acaaaaacct ttccgccgtc
      121 attcccgcgc aggcgggaat ctggaaaccc aatgctgcaa gaattgatta aaaacaactg
      181 aaaccgagca aactggattc ccgcctgcgc gggaatgacg aaataaggtg agtaaaactt
      241 attaaataaa ttaattaaaa acaataactt atattatttt ctttaaattt tcgataaaat
      301 caattttccg tattcaatac tgctccaatc aattcctgcg caccgtcgtc cgccagtttt
      361 ttggcaaccg cacgtccaag cgcatcggca tatccggcag gggcttgcgc gtccgcctgc
      421 aacacaatcg atccgtcggg atgcccgacc aatccgcgca agatcagcag cccgttttct
      481 tcagtgcaat acgcggccag cggcacttgg cagcttccgc ccaaagcgcg cgccaaagcg
      541 cgttcggcgg taacgcaggc gtgtgtggta tcgtggttta acggcttcaa gacttcgtac
      601 aaatcttcgc gatgcgtggc aatttcaata cccaatgcgc cttgtccggc ggcaggcagg
      661 ctgtcggatt ccgacaaaat catgcggatg cgttcatcca attccagacg ctgcaacccg
      721 gcggcagcca agataattgc atcgtattcg ccgttgtcga gtttggacaa acgggtttgt
      781 acattgccgc gcaggggttt aatcaccaaa tgcggatagc gcgcgcgcaa ctgggcttcg
      841 cggcgcaggc tggatgtgcc gacaaccgcg ccttcgggca tttcttccaa acgcgcgtat
      901 cggttggaca caaacgcgtc aaacggattg gcgcgttcgc tgatggcggc aagggcgaag
      961 ccttcgggca aatccatcgg cacgtcctta atcgaatgca ccgccaaatc ggcgcgcccg
     1021 tcttgaaggg attgttccaa ctctttgaca aacaagcctt tcccgccgac ttttgacaaa
     1081 gttctgtcca aaatccggtc gccgcgcgtg gtcatgccca aaatttcgac ttcgcaatcg
     1141 ggatacagcg ccttcagacg gccttggata tgtttcgcct gccacatggc aagcaggctt
     1201 tcgcggctgg cgataacaag ttttttcggg ttcatgttca atgcgttcaa agaatggaaa
     1261 cggcgtggag tctatcatat tttgaacgtt tcttcagacg gcaccgtcag gtctatgtta
     1321 tatttacgcc cacaaaacac gaaacccgcc acaatgaaac gcgccaaaaa ataccctttc
     1381 ttaacgttgc agcggcaacg tttccatttg aactttgaaa acgcctcttc cgccgccggc
     1441 atccccgccg aacgcgattt ttaccgctgg gcgtggtctg ccttgaaaaa cgaatacctc
     1501 cgcgccgaca tcggtttgat tcttttggac gaagaagaag cccgcgccta caaccgcgac
     1561 taccggggca aagattatgc caccaatgta ttgagtttcg cgctcaacga aggcgaaatc
     1621 ctgccctgcc aagtttcgga aagactgtac ggcgatttga ttatctgccc gcaagtcgtt
     1681 ttaaaagaag ccgccgaaca gggcaaaacg cctgaacggc attttgccca cttaaccata
     1741 cacggcacgc tgcacctgat gggatacgac cacatcaaag acgatgaggc cgaaataatg
     1801 gaagccgaag aaatccgcct gatgcgggcg gcaggctatc ccaaccccta ccgagaggac
     1861 ggacattaaa atggacggcg cacaaccgaa aacaaatttt tttgaacgcc tgattgcccg
     1921 actcgcccgc gaacccgatt ccgccgaaga cgtattaaac ctgcttcggc aggcgcacga
     1981 acaggaagtt tttgatgccg acacactgac ccggctggaa aaagtattgg actttgccga
     2041 gctggaagtg cgcgatgcga tgattacgcg cagccgcatg aacgtattga aagaaaacga
     2101 cagcatcgaa cgcatcaccg cctacgtcat cgataccgcc cattcgcgct tccccgtcat
     2161 cggcgaagac aaagacgaag ttttgggcat tttgcacgcc aaagacctgc tcaaatatat
     2221 gttcaacccc gagcagttcc acctgaaatc cgtcttgcgc cctgccgttt tcgtgcccga
     2281 aggcaaatct ttgaccgccc ttttaaaaga gttccgcgaa cagcgcaacc atatggcaat
     2341 cgtcatcgac gaatacggcg gcacgtcggg tttggtcacc tttgaagaca tcatcgagca
     2401 aatcgtcggt gacatcgaag acgagtttga cgaagacgaa agcgccgaca acatccactc
     2461 cgtttccgcc gaacgctggc gcatccacgc ggctaccgaa atcgaagaca tcaacgcctt
     2521 tttcggtacg gaatacggca gcgaagaagc cgacaccatc ggcggcttgg tcattcagga
     2581 attgggacac ctgcccgtgc gcggcgaaaa agtccttatc ggcggtttgc agttcaccgt
     2641 cgcccgcgcc gacaaccgcc gcctgcacac gctgatggcg acccgcgtga agtaagcaga
     2701 gcctgcccgc accgccgttt ctgcacagtt taggatgacg gtacggtcgt tttctgtttc
     2761 aatccgcccc atccgccaaa cataaaaatg ccgtctgaaa ccggaaaaca ggttcagacg
     2821 gcatttcgct tatccgctta ttttttgctc aaagtcagcc ccgtccagcc gaataacagg
     2881 gttaaagcca aactcaaata gcagaaaaag gcataaggca gatattccca aacgggtacg
     2941 ccaagggcgt ggctgataaa tacgccgcac acgctccacg gcacgagcgg gttaatcacc
     3001 gtccccgcat cttccagagt ccgcgacagg ttgcgcgaat gcaggccgag tttgtcgtaa
     3061 acgggtttga acgtttctcc cgaaagcagg atgctcaaat attgctctcc aatcaggaaa
     3121 ttgaccccga ccgaagtcat ggcaacgctg aacgtcgcgc gtccggcatt cgtcaagaag
     3181 gtacggacgg cctccagcag ggaaggaatc acaccgagcg caaacagcag cccgcccaaa
     3241 ctcataccga ggataacgat ggtctgcgta aagaacatac tctccaagcc gccgcgcgaa
     3301 atcagtttgg caatgtcttt aaacgcttcg ccttcgagtt tgtagccgcc ataaaaccac
     3361 gcgccgagct gacgcagatc gggcgtgctg tgcagatacg ttacggcaac ggcggcaatg
     3421 acggtaaaga gcatggcgac cacggcattg acgcgcatca atgccaaaac gaccaacagt
     3481 gcaaacggaa tcagcgaata gccgtgcacc aatcccgtgg cttcaagctg gctgcggaag
     3541 gattcgacgc tgttcaaatc ctgcgcggcg acgctgggaa gaagccaaag catcagtgcc
     3601 gcgctgataa gccacgcggg gatggtggtg tacatcatgt ttttgatgtg ttcaaacagg
     3661 tcgataccga cgatggacgc ggaaatgccc gtggtgtcgg aaagcgggga cattttatcg
     3721 ccgaaaaaca caccggaaac aatcgcgccc gccgtcatcg ccatatcggc ctgaaacgcc
     3781 gccgccatcc ccataaaggc aacgccgaca gtggcgcagg cggtcaggct gctgccgatg
     3841 gacacgccga tgacggaaca cagcgcgaag gcggaaaaat aaaaataagt cggggaaatc
     3901 agcccgaaac cgtaatacat cagcgtcgga atcgcgccgc tcatcatcag cgcgctgacc
     3961 atcagcccga tgaagaaaaa caggtaaacc gcgcccatac cctgattcaa cgcgcctatc
     4021 atccctgcct gcatatcgtt gtatttcaaa ccgcgcgcca agccgtacaa aatcagcacg
     4081 acgatggcgg caataatgga catatgcggc agccactcca atgaaatgat ggtatagccc
     4141 attgcggcaa tcagagcgac gacgacggca agtgcctcac cgcgcggcat atcgagtaag
     4201 gatttgaaag caaacatgat tctttgcccg atttgatata aagttaacgg attctaacac
     4261 accatgccga atctgcccga cgttttcaga cggcatttta gcgttttaca aaagccgggc
     4321 agaaacaaaa cggcaatttt ctcgatttaa attaattttt ttgaaatata atttttattt
     4381 ttttatttgt tggcatataa gacttttatg gattgaagta ttaaaacctc ggcacacacg
     4441 ccgcaaacac cggtacgcaa aaatgccgtc tgaaagatgt tcagacggca tttctcaaga
     4501 aaaagattat ttgtccgccc tatatccgta caccgcaaaa aacacgatat acaggtaaca
     4561 gataacggaa acgacaaacg aagacatcag ggtgtaagta tccgccgccc aaccttgaat
     4621 taccggcacg accgcaccgc cgacaatcgc ggtacacagc acaccggaag ccgcgttggt
     4681 gaatttgccc aaccctttgg ttgccaaaga gaaaatcgtc gggaacataa tcgagttgaa
     4741 aaaaccgatg gcaagcagcg accacatcgc cacatccgca ttgccgctgc ccgtcgccat
     4801 cgcgacggca agcagcacga ccgcagccga tgcgttaaac gccaaataac ggttgggcgc
     4861 gaatttcgcc attaccgccg aaccgaggaa acgtccgacc atcgcaccgc cccaataaaa
     4921 cgacaggtaa tgcgcggcag aagcatgatc cagccctttc agataaccca acacattgac
     4981 catcagcgaa ccgatagaca cctccgcacc gacgtagcag aaaatgcctg ccgtaccgaa
     5041 cacgagatgt ttgtattgcc atacgccggt tttgccgtcg tggttgtgcg cgctttcctc
     5101 ggcggcaatt ttgcgcgcgt cgggcagccg gatcattttc acgaaaacgg caaggataat
     5161 cagcagcccc gccagtccca aatagggaat ctgtacggaa gaaatctgtt ccgccttgct
     5221 gacggtttgg gttgcgtccg ccaaaatcag gaacgcgccg atttgaggcg caatggtcgt
     5281 acccaaagcg ttaaacgcct gaaccagcgt cagcgttgcc gattccttgc cgggtttcgc
     5341 cagcagggta acataaggat taccggcgac ctgaagcagc gttacgccgg aagccaaaat
     5401 aaacaacgcg cccaaaaata ccgcgtaaga atggctgccc gcagcaggat aaaacagcag
     5461 gcacccgacc gccgtcagca gaaagccgcc gataacgccg tttttgtagc cgactttgcc
     5521 gacaaaagcc cccatcggga tggacattac cgcataggcg gtaaaaaagc aaaattgaat
     5581 cagcatcgcc tgaacgtaag agaggtcgaa aatttctttc aaatgaggga taaggatgtc
     5641 gttcatgcag gtaataaaac ccatcatgaa gaacagcgtg gtcaagacga ccaatgggga
     5701 cgtatggttg ttttgtgatt gcgcagacat gatttgcctt tcgtggtgtc ggaatattgc
     5761 ccgtttcaag aaaatagaaa ttgacatttc ttaacggcaa tgcaaatcat actgatattt
     5821 gaacattttg caaactttat cggatgcagg caatgcgccg atctgaaatt caagcagcag
     5881 aaattaatat tttacagttg tttgcatagg ttttaataga ttttaaaaaa ttccggcggg
     5941 cttgcaaaaa ccgttcccaa taaaatgaaa atcctccgcc gtatccggaa aattcggaaa
     6001 tgccgcatcg gggcatcaaa accggagcgg aaagaatgtc gtatgcccga caaaaaaggg
     6061 gcatacggct gtgccgtatt tttgcaccgt cctccgccct tcggcggggg cgtgaaaata
     6121 ccgtataatc cgtcgccgat ttttacctgt ttgggactgt ttgcaatgat accctcctac
     6181 acctacgccg ccctcgcttt ttccgccttc acttccgcca ccctcctgcc cggcacgtcc
     6241 gaagccgcct tcgccctgtt cgtccgcaac ttccccaaac acgcatacgg cgcgttgctg
     6301 tgcgccggcc ttgccaacgg attgggcagt atggtttcct attggatggg acgtttgctg
     6361 ccttcccgaa aaatgccgtc tgaaaaaaca ctgaatctga tacggcgttt cggtatttgg
     6421 ctgcttgcgt ttgcgtggct gcccgtcgtc ggcgacgcac tgccgcttac cgccggctgg
     6481 ctgcggttga atccgtggac aagcgggctg atgctggtta tcggcaaaac ggcgcgttac
     6541 gccttcattc tgtggggaat gcaatattac gccgcctgaa ccgtgcgtta taatgccgac
     6601 cgcacaaacc gactgcacaa aaaaaatgaa cagacaaatc cgccaagaaa tcttcgagcg
     6661 tttccgcgcc gccaaccccc atccgaccac cgagctgaat ttcaactccc ctttcgagct
     6721 tttaatcgcc gttctgcttt cggcgcaggc gaccgatgtc ggcgtaaaca aggcgacggc
     6781 gaagctgttt ccggttgccg atacgccgca ggcgatgctg gatttgggct tggacggcgt
     6841 gatggaatac acgaaaacca tcgggctgta taaaaccaag tccaaacaca ttatgcaaac
     6901 ctgccgcatc ctgctggaaa aatacaacgg cgaagtgccg gaagaccgcg aggctttgga
     6961 atcgttgcct ggcgtggggc gcaaaacggc aaacgtggta ttgaacacag cgttcggaca
     7021 ccccgttatg gcggtcgata cgcatatttt ccgcgtgtcc aaccgaacca aaatcgcacc
     7081 cggaaaagat gtacgcgaag tcgaagacaa actgatgcgc ttcattccta aagaatttct
     7141 gatggacgcg caccactggc tgattttgca cggacgctac acctgcaagg ctttaaagcc
     7201 gcaatgccaa acctgcatca tcaacgattt gtgcgaatat cccgccaaat cctgaccgcc
     7261 gccgcccttc aaaatgaaac gcttgaaaat tcggacggac gttgtagaat ttcaacctat
     7321 gccgtctgaa gccgacaaaa tgcgcttcag acggcattaa agccggtacc ggcatccgac
     7381 aaaggacaaa acgtgttcaa aaaataccaa tacttcgctt tggcggcact gtgtgccgcc
     7441 ttgctggcag gctgcgaaaa ggcaggcagc tttttcggtg cggacaaaaa agaagcatcc
     7501 ttcgtagaac gcatcgaaca caccaaagac gacggcagtg tcagtatgct gctgcccgac
     7561 tttgcccaac tggttcaaag cgaaggcccg gcagtcgtca atattcaggc agcccccgcc
     7621 ccgcgcaccc aaaacggcag cggcaatgcc gaaaccgatt ccgacccgct tgccgacagc
     7681 gacccgttct acgaattttt caaacgcctc gtcccgaaca tgcccgaaat cccccaagaa
     7741 gaagcagatg acggcggatt gaacttcggt tcgggcttca tcatcagcaa aaacggctac
     7801 atcctgacca atacccacgt cgttgccggt atgggcagta tcaaagtcct gctcaacgac
     7861 aagcgcgaat ataccgccaa actcatcggt tcggatgtcc aatccgatgt cgcccttctg
     7921 aaaatcgacg caacggaaga gctacccgtc gtcaaaatcg gcaatcccaa aaatttgaaa
     7981 ccgggcgaat gggtcgctgc catcggcgcg cccttcggct ttgacaacag cgtgaccgcc
     8041 ggcatcgtgt ccgccaaagg cagaagcctg cccaacgaaa gctacacacc cttcatccaa
     8101 accgacgttg ccatcaatcc gggcaattcc ggcggcccgc tgttcaactt aaaaggacag
     8161 gtcgtcggca tcaattcgca aatatacagc cgcagcggcg gattcatggg catctccttt
     8221 gccatcccga ttgacgttgc catgaatgtc gccgaacagc tgaaaaacac cggcaaagtc
     8281 caacgcggac aactgggcgt gattattcag gaagtatcct acggtttggc acagtcgttc
     8341 ggtctggata aagccagcgg cgcattgatt gccaaaatcc ttcccggcag ccccgcagaa
     8401 cgtgccggcc tgcaggcggg cgacatcgtc ctcagcctcg acggcggaga aatacgttct
     8461 tccggcgacc ttcccgtcat ggtcggcgcc attacgccgg gaaaagaagt cagcctcggc
     8521 gtatggcgca aaggcgaaga aatcacaatc aaagccaagc tgggcaacgc cgccgagcat
     8581 accggcgcat catccaaaac agatgaagcc ccctacaccg aacagcaatc cggtacgttc
     8641 tcggtcgaat ccgcaggcat tacccttcag acacataccg acagcagcgg caaacacctc
     8701 gtcgtcgtac gggtttccga cgcggcagaa cgcgcaggct taaggcgcgg cgacgaaatc
     8761 ctcgccgtcg ggcaagtccc cgtcaatgac gaagccggtt tccgcaaagc tatggacaag
     8821 gcaggcaaaa acgtccccct gctggtcatg cgccgtggca acacgctgtt catcgcatta
     8881 aacctgcaat aacgcacata atttaaccaa aatattttta aatgataaaa tgcccgtcaa
     8941 taccgattaa cgggcatttt acaaaaatca gttggttgat tttaaacaaa tattttttac
     9001 gacaaaaaat atttccaccc attttgccaa caaaacccaa accggaggcg gacagatacc
     9061 cacccatccg gacgcttaac accccccata aaccatgcag ctgattgact attcacattc
     9121 atttttctcg gttgtgccac cctttttggc actggcactt gccgtcatta cccgccgcgt
     9181 actgctgtct ttaggcatcg gtattttggt cggcgttgcc tttttggtcg gcggcaaccc
     9241 cgtcgacggt ctgacacacc tgaaagacat ggtcgtcggc ttggcttggg cagacggcga
     9301 ttggtcgctg ggcaaaccaa aaatcttggt tttcctgata cttttgggca ttttcacttc
     9361 actgctgacc tactccggca gcaatcaggc gtttgccgac tgggcaaaac ggcacattaa
     9421 aaaccggcgc ggcgcgaaaa tgctgaccgc ctgcctcgtg ttcgtaacct ttatcgacga
     9481 ctatttccac agcctcgccg tcggtgcgat tgcccgcccc gttaccgaca agtttaaagt
     9541 ttcccgcgcc aaactcgcct acatcctcga ctccactgcc tcgcccatgt gcgtgctgat
     9601 gcccgtttca agctggggcg cgtcgattat cgccacgctt gccggattgc tcgttaccta
     9661 caaaattacc gaatacacgc cgatggggac gtttgtcgcc atgagcctga tgaactatta
     9721 cgcgctgttt gccctgatta tggtattcgt cgtcgcatgg ttctccttcg acatcggctc
     9781 gatggcgcgt ttcgaacagg ctgcgttgaa cgaagcccac gacgaaaccg ccgcttcaga
     9841 cgctaccaaa ggtcgtgttt acgcattgat tattcccgtt ttggccttaa tcgcctcaac
     9901 ggtttccgcc atgatctaca ccggcgcgca ggcaagcgaa accttcagca ttttgggggc
     9961 atttgaaaat accgacgtaa acacttcgct ggtattcggc ggcacttgcg gcgtgcttgc
    10021 cgtcgtcctc tgcacgttcg gcacgattaa aaccgccgat tatcccaaag ccgtgtggca
    10081 gggtgcgaaa tccatgttcg gcgcaatcgc cattttaatc cttgcctggc tcatcagtac
    10141 ggttgtcggc gaaatgcaca cgggcgacta cctctccacg ctggttgcag gcaacatcca
    10201 tcccggcttc ctgcccgtca tcctcttcct gctcgccagc gtgatggcgt ttgccacagg
    10261 cacaagctgg gggacgttcg gcattatgct gccgattgcc gccgccatgg cggtcaaagt
    10321 cgaacccgcg ctgattatcc cgtgtatgtc cgcagtaatg gcgggggcgg tatgcggcga
    10381 ccactgttcg cccatctccg acacgaccat cctgtcgtcc accggcgcgc gctgcaacca
    10441 catcgaccac gttacctcgc aactgcctta tgccctgacg gttgccgccg ccgccgcatc
    10501 gggctacctc gcattgggtc tgacaaaatc cgcgctgttg ggctttggca cgaccggtat
    10561 tgtattggcg gtgctgattt ttctgttgaa agataaaaaa cgcgccgacg tttgaccttc
    10621 ccccctgttc agacggcata tgccgtctga agttttttgg agcaaaccgc aatgaccgtc
    10681 ccccatattc cgcgcggccc cgtcatggca ggcattgccg ccttccgcct gaccgaagag
    10741 gaaaagcaac gcctgctcga tcccgcaatc ggcggcatca tcctcttccg ccgcaacttc
    10801 caaaacatcg aacaactcaa aacactcacc gccgaaatca aagccctgcg cacacccgaa
    10861 ctcattatcg ccgtcgatca cgaaggcggc agggtgcaac ggtttatcga aggttttacc
    10921 cgcctgcccg caatgaacgt tttagggcaa atttgggaca aagacggcgc gtccgccgcc
    10981 gaaaccgctg ccggacaagt cggccgggtt ttggcaaccg agctttccgc ctgcggcatc
    11041 gacttgtcct tcacgcccgt cttggatttg gactggggaa actgcgccgt catcggcaac
    11101 cgcagcttcc accgcaatcc cgaagcggtc gcccgcctcg ccctcgccct tcaaaaaggt
    11161 ttggcaaaag gcggtatgaa atcatgcggc aaacatttcc ccggacacgg gtttgtcgaa
    11221 ggggacagcc atttggtgct acccgaagac gggcgcagcc tcgacgaact cgaagccgcc
    11281 gacctcgccc ccttccgcat tatgagccgc gaaggtatgg cggcggtcat gcccgcccac
    11341 gtcgtttatc cgcaagtgga cacaaagccc gcagggtttt ccgaaatctg gctgaaacag
    11401 attttgcgcc gcgatatcgg attcaaaggc gtgattttct cggacgattt gaccatggag
    11461 ggcgcgtgcg gagtcggcgg catcaaagaa cgcgcccgca tttcctttga ggcaggttgc
    11521 gacatcatat tggtctgcaa ccgtcccgac ttggtcgatg aattgcgcga cggtttcacc
    11581 attcccgaca accaggattt ggccggacgt tggcaataca tggaaaactc gctcggtcat
    11641 gaggcggtac aagccgttat acagacaacg ggtttccaag cggcgcaagc ctttgttgcc
    11701 ggccttgcct cgccgcagga cacggcgggc ggcgtaaaag tcggagaggc cttttaaatc
    11761 cgcccgacac gcggcaaaaa ggcaaaacgg tataggcacg ccgtattttt tgcgctataa
    11821 tcacacctta attaattcgg aatgtagcgc agcccggtag cgcacttcgt tcgggacgaa
    11881 ggggtcggag gttcgaatcc tctcattccg accatattca gaccgcctcg acatcgattg
    11941 ccggggcggt ttttcccttt tggcggcaac aaaaaaatgc cgtctgaaac agaatgctgt
    12001 ttcagacggc atttcgtttt ccccaatcct gccatttgcg cgcactgaca aagggtatcg
    12061 gcaaggttca aaagaccatc ggaatctagg aagcctggcg tgtacccaac cgcggcatct
    12121 tattcattaa ggcttcgtcc tgattgggcg gcggaaatgt cggcagtttc acgcggaagc
    12181 ggcacacttt ccccgtatgt caaacatcgg caagaaggta cgcctgccct ttgggttaca
    12241 ctgcttaaac tactctattc ccgcccattt gtgcgtctgc acgctcaact gccaatgcac
    12301 gggcgcgtcg gggcggctgt ttaaaatacc gatttggcgg atggtgtcgt agatgttcat
    12361 cacgccgttt tgttcgcagg gagaaagata gtaatggcgc gcacggattt tgcgttccat
    12421 attttcgcaa aacgcaagga catcgccgtc ggcaacaatc cgtacttcgt cggcttcggc
    12481 gatgcagttg gtttcatact tggcggcgta gcaggctttg gggctggtgg cgacatagtc
    12541 gatttgcggc ggcgcgggct tgagcccgtt ggtttcgaga cagaggaaat agccttccgc
    12601 cttgagcgtg tccagcagca tatcgagatg cggctggatg gtcggctctc cgccggtgat
    12661 gatgatgttg cgggcggcat aagttttcag acagcctaag atgtcggaca ggctcatcat
    12721 accaaatgtc aaataatcgg tatcacacca gccgcacgcc agattgcatt tgcccaagcg
    12781 gacgaaaacg gcaggcatac ccgtgtttcg gccctcacct tgcaggcttt caaagatttc
    12841 gacgatgcgg tactggggat tttcgggggc gacgctgatt tttttcatac cgccaacacc
    12901 gccgccgcca aaaatgcggg caaaccttgt ttgacgagta tgcctttgtt gccggacgaa
    12961 aacgcacccc acgcggcggc aatcaggacg aagccgagaa acagtaccgt cgcgccgtac
    13021 acggcattat ccggagcggc aaactgcgcc cacaaaagcc cgacggcaag aaagccgttg
    13081 tacagccctt ggttgccaaa cagggtctgc acgcgtttct gttccataaa ttcgtaaggc
    13141 agcttgaaca tttccgccgc tttttcgccg ggaatctgtg tcatttcaag ccaggcgatg
    13201 tagaaatgtt cggcggcgac gaggagaact aagagggtgg aggggaattt catgggtgtt
    13261 ccttttaatg ggtgtgtcgg tatggaatgc cgtctgaaga acggagaatc cgcacaggag
    13321 aaaaaccgct ttgcggtaaa ttcaaataag gacgctccac actgtcattc ccgcgcaggc
    13381 gggaatccag agatttggaa ttgctgcaat ttttaaatat tcctgataaa tcaaggtctg
    13441 gattcccgcc tgcgcgggaa tgacgcggtt gggcatgtct tatttgaatt tactatatcg
    13501 atacgttcaa gattttccct actccccttc atactccgcg cacgatgtcg gcgtttccca
    13561 caatttgacg cggcacacgt tcagccccgc gtttttcaaa cgtccgtaca tttcaaccgc
    13621 catattttcg gcagttgtgc ggcagggcag gcgcagggtt ttcatattcc agccttccaa
    13681 aagcgcggcg atttgagatt cgcgcccgtt gccgccgtgg tagatgaagg cgtggtcgaa
    13741 atggtcggta atgtgttgtt tcacaatggc tttcaagtcg gtaaagtcca tcaccatacc
    13801 gtctttcgct ccgcctttga tggtgccgtc tgaaacggtg atttcgagtt tgtaggtatg
    13861 tccgtgcagg ttttggcatt tgccgtcatg cccgtcgagc atatgcgagg agtcgaaggt
    13921 aaatatcttg gtaattttca tcccgattcc ttttcagtga aaaccccgcc gctttccgcc
    13981 cggagggtgg cttgatgtgt cgttttgcgt ggaaacatat ccgctgcttc aaatgggcaa
    14041 atagccttga aagccgattt cggcgggttt gccgtgcgga ttcggggtca ggcggatatg
    14101 gaaattgccg atgtgctcga gcgcaagcag gttgtccacg tctttcaagt tgtctttatg
    14161 ctgccgaaca tagtcggtca acagggtttg cagggctttt tgtttggctt cggcaggcgt
    14221 ggcggcggcg aacagcccga acgcgtgcgc ctcggcaagc tgccccgcgc gatagccgcc
    14281 gacattggca aaatacaggc gcggagcgtt ttcaggcggc attttcgttt tgggcgtttc
    14341 ggatacggcg atgtcgtaac cgtccgccca ttcgacaacc tgccaaccgt cgatgtggat
    14401 tttgtccgta tcgccgaacc acgcggcttt gagtgcgggg acggcttcgc ggtagtcgtc
    14461 gcacacggca aattggatgt cgtgcacttc gatattcgac cggccggcat tgccgccgag
    14521 gtaaaacata tggagcttgg gcatatcgta ttccttgtgc tttgcgcgcg ttcagacggc
    14581 ctttttactt tccagacatt ccgccagccc gcgttcgcgc aagatacagc tcgggcattc
    14641 gcggcagccg ccgacgatac ccttgtagca ggtgtgggtc tgttcgcgga tgtagtccaa
    14701 cacgcccatt tcgtccgcca acgcccacgt ttgcgccttg gtcaggtaca tcagcggcgt
    14761 gtggatttga aaatcgtagt ccatcgccag attaagggta acgttcatgg atttgacgaa
    14821 cacgccgcgg cagtcgggat agcccgaaaa atcggtttcg cacacgcccg cgatgatgtg
    14881 ccggataccc tgccctttgg caaaaatggc ggcgtaaagc aggaaaagcg cgttacgccc
    14941 gtccacaaag gtattgggaa cgccgttgtc ggcggtttcg atggcggcgg tttcgtccat
    15001 cagggcgttg tgcgtaatct gccgcatcag gctcaagtcg agtacggttt gtttgacgcc
    15061 caaatcctgc gcgatccact ctgcgcgttc cagctcgacg gcatggcgtt gcccgtatcg
    15121 gaaggtaatg gcttggacgt tttcgcgccc gtaggtttgg attgcctgaa tcaggcaggt
    15181 ggtcgaatcc tggccgcccg agaagatgac caaggctttt tggtttgaca tatcgaatcc
    15241 tagttttggt aacgcgggag gtttgcgaac cgcgaagttg gacagtaaag acgcggatta
    15301 tacaggcaaa cgcgttcaga cggcatctga acgccatgcc gtctgaacac gcggcgtatg
    15361 ccgacttgac ggcgagttcc gccaagcgtc tgccttgtgc gaaggcgata ttgttttctt
    15421 cggcggtcag gacgggagcc gctgtcggga atatctttat ttgacggctt cgcagacggc
    15481 ggacactttg gggacggtgc gcaatacggc ttcgcaacct tcaacgctgt cgatgccgcg
    15541 agtgattcgg cgtgcgggat tgcgggtgct gccgttttgg gaatagtaga ggacgaggat
    15601 tttgagggga tttgggttca tgggcggatt ccgttaaaat agtttggtgt tcagacggca
    15661 taacggttgt ccagatgccg tctgaaacag tttgaaaaca tgaaacctgg taagagatta
    15721 tgaccttttt acaacgttgg caaggtttgg cggacaataa aatctgtgca tttgcatggt
    15781 tcgtcatccg ccgtttcagt gaagggcgcg taccgcaggc agcggcgagc atgacgttta
    15841 cgacactgct ggcactcgtc cccgtactga ccgtaatggt cgcggtcgct tcgattttcc
    15901 ccgtgttcga ccgctggtcg gattcgttcg tctccttcgt caaccaaacc attgtgccgc
    15961 agggcgcgga tatggtgttc gactatatcg acgcattccg cgatcaggca aaccggctga
    16021 ccgccatcgg cagcgtgatg ctggtcgtaa cctcgctgat gctgattcgg acgatagaca
    16081 atgcgttcaa ccgcatctgg cgggttaaca cgcaacgccc ctggatgatg cagttcctcg
    16141 tttattgggc gttgctgact ttcgggcctt tgtctttggg tgtgggcatt tcctttatgg
    16201 tcgggtcggt tcaagactcc gtactctcct ccggagcgca acaatgggcg gacgcgttga
    16261 agacggcggc aaggctggct ttcatgacgc ttttgctgtg ggggctgtac cgcttcgtgc
    16321 ccaaccgctt cgtgcccgcc cggcaggcgt ttgtcggagc cttgattacg gcattctgcc
    16381 tggagacggc acgtttcctg ttcacctggt atatgggcaa tttcgacggc taccgctcga
    16441 tttacggcgc atttgccgcc atgccgtttt tcctgctgtg gttaaacctg ctgtggacgc
    16501 tggtcttggg cggggcggtg ctgacttcgt cgctgtctta ttggcagggc gaggccttcc
    16561 gcaggggatt cgactcgcgc ggacggtttg acgacgtgtt gaaaatcctg ctgcttctgg
    16621 atgcggcgca aaaagaaggc cgaaccctgt ccgttcagga gttcagacgg catatcaata
    16681 tgggttacga tgaattgggc gagcttttgg aaaagctggc gcggtacggc tatatctatt
    16741 ccggcagaca gggctgggtt ttgaaaacgg gggcggattc gattgagttg agcgaactct
    16801 tcaagctctt cgtgtaccgc ccgttgcctg tggaaaggga tcatgtgaac caagctgtcg
    16861 atacggtaat gacgccgtgt ttgcagactt taaacatgac gctggcggag tttgacgctc
    16921 aggcgaaaaa acagcagcag tcttgaatat ttttgaaact gtatttttat cctaaagaca
    16981 ttttcctatc tgctgattcc accgtttcct ttctgtgtgc cgccataatt tttatgatag
    17041 acaagccatt caagtttgca ttgactgctt tttcattggc aatatccaaa tgactggccg
    17101 cttcatctaa aaataggatt ttcggttcgc aatataaggc ccgcgccaat acgatacgtt
    17161 gtttttgtcc gcctgacagt gcgctgccca tatcgccgat caaggtttca tagcccatag
    17221 gcatggcgga gatttcttcg cgaatcatag caaggcaggc gcaatgttcg atttttcctc
    17281 tatccggttc tgtatcgaaa aaacagatat tctctgcaat tgagccagca aatgcaaata
    17341 tagtggatta aatttaaatc aggacaaggc ggcgaagccg cagacagtac agatagtacg
    17401 gcaaggcgag gcaacgccgt accggtttaa atttaatcca ctataaaaca tcgtcctgcc
    17461 tgaccatcgc actcaaattg cggataaaac gtggcggtaa gctgtaaatg tcgtgcccat
    17521 ttatcatgac tttgcctgat tcgggaggta gattgccgct taaaatatcc aaaagtgtcg
    17581 atttaccgca gcccgatcgt ccgattaacg caagcgattc accacgtccg atcttgaagc
    17641 tgacgttttg taaaacatat ggctcgttat cggcataacg gaaagaaaca ttttccactg
    17701 agatttcgat gtcgtctgaa atatcaggaa tgcctacatc cggcgtagct tccgattctg
    17761 tttcattcaa tacggtgtca gctaaccgtt ccgcatgaag tgacagcatt ttaatttgca
    17821 caaactgatc gaccagcgag ccaatacgtc cttcaaactg tcccttataa gccaaaaaag
    17881 ccattagtac gccgacggta aagacgccgt ccaataccat gcccgcagct aagtaaataa
    17941 tcaaaatatt tgccaaaccg aaaagtagct tatttgccaa gccgaaccaa attcccagtt
    18001 tctgcaccgt cagtcccgta ttaaccgtat ccaccagcag ccccatccat gcagaatatc
    18061 gttgaggacc tttaccaaat tgcttgactg ctcggatacc gcgtaccgtt tccataaaat
    18121 agctgctttg tttggcagca tggatgatgt tttcctccgt cgcccgtcgc aacggataat
    18181 aggctaccca acggactgcg atataaacta ccagtgtcgc tgcaaccaaa cctgtcagta
    18241 tcgggctgta cactatcatt agcacaaaag taaaaacagc cgttaggctg tttaaaacca
    18301 acacgaaaaa cgttgaagtc agagttccct gaatactatc caccgccccg aagcgcgata
    18361 ccacatcgcc caaatgccgt ttagcgaaat aatctgtagg caaatctatc aaacgtttga
    18421 aaacgtttga tttccactga acatttaacg tcgttgaaaa atgcatgccc aaccagccct
    18481 gcatgacaga caccgtattt tgtaccagaa gtagcaaggt aaagcccaaa gccagcgtcg
    18541 ctaatagatt acgatcgccc gttaccacta catggtcgat gacccattgc ataaaaaacg
    18601 gcgacaccaa agcaaacact tccattgctg ctgccaaagc caaaagctga aacaatgtcc
    18661 gccccagccc agaaatcccg cgcaacatgg gtaggatgcg gatttcctgc ttttcttccc
    18721 ctgcctcgaa acgcgtgttt ggccacagtt ccaaagcaat tcccgtaaac ttgcgcgata
    18781 tttcctccgt cttgactttg cgtcgtcccg aagccggatc catgacggca gccccgtccg
    18841 aagataccga ttccagcacc acaaaatgat tcaaattcca atgtagaata cagggcaggc
    18901 gcaaactgcc caattcgtct aaatccagcc tcaacgcccg tcccgtcagc cccatatcat
    18961 cagcaaaacg aacaatatct gccaaattcg cacccttaag tgacagacag tattttgaac
    19021 gcagtgcgcg caaatccgta tagaaaccat aaaatccggc cacagccgct agacatgcca
    19081 agccacactc cgctacttct gtttgcagta taacgggcag cttttttgtc aagcccaaag
    19141 acaggttttg cagataatcc atagatttcc ttgttgaatc agaaaagaga gatgggaaga
    19201 cgggaatggt ttggaaaggc cgtctgaaaa ggtttcagac ggcctgcagc cccatattgg
    19261 ctttcaatgc gaccggtttt cctcacgttt caaatacagc agatacgcaa gaaaagacaa
    19321 agcccctaca ggtcctgcga cggtaaccga aaaaaacctg cctttgtaaa acacaccgtc
    19381 gaagtttaaa aaatagcaaa ggatgtcggt caggatgctg atggcaagat aaatcagcac
    19441 agcggctttc cagtgtttca ggtaatgtgc gaaatccatt tcaaactccc gatcaacagt
    19501 tgcacgggtc acaatggcag tcttctccgc attggcagtt gcgcctgtcg atcatttttc
    19561 cccattttgc accggcaaaa ccgccgatat gcccacccaa tgcagaaccg ccctcagctc
    19621 ctacccatcc gccgcctgcg ccgccgatgg caattccggc aggatttccg cctgttacca
    19681 atgcgccgcc cgccattccc gtgaatgctc cggcaaacga ccctaaagcg ccgccgacct
    19741 tgcttccgac ccattctccg gagactctgc ccctgtgccc ccaagtacct tctgtgccgc
    19801 cgaaaacagc ggtcatttcg ttcatggtca attctctcat ttcaaaactc cttttatcgt
    19861 caatcgaaaa attgaaaggg cagataccct atccttgctt ttacagacag agtatctgta
    19921 aaagggggta gtttcaggca gcttcagtgt ttatgttttt atgtagtgtt ttacaggtcg
    19981 tctgaagagg tttcagacga ctttttatct ttcagcctca agccttacaa cctgcccgac
    20041 atactataaa tcggctccaa tacccattcg tacagccgcc gtttctcgtg taggatgtct
    20101 gcttccagcg tcatgccgat ttgcagcggt ttttcctcac cgtatgcagt gatggttggt
    20161 ttgtcgggtt ttattttcac gagataaaca ggttcgttgc tcttcgccaa atcggaggat
    20221 accatgccca atcccgacaa ttcctgtctg cccagtgccg tttttgccac tgatacgaca
    20281 ctgccggaag caagcccgaa tttctgatag ggatatgcct gataacgtag gacaaccttg
    20341 tctttcggct tgataaagcc tgctgcactg ctggggatat atagatgggc atataactcg
    20401 gtacgttcgg gaacaatgct caagagcagt ttggaaggat caacctgccg tccgacttcg
    20461 acgttcggta ttgctatata acccgaccgt cctgcacgga tgatttgttc agagcgcatt
    20521 tcaaaatcca aaacttcttg agaaatatcg gcaatggtgc gttcaagctg gctttgttct
    20581 gtctcatgcc gtttggggag gctggccaat gtcagattct gcgtgcggat ttcctgaagc
    20641 agcccggctt cttctcggcg gtaggcatca agtttggctt tctgctctaa aagctctgcc
    20701 tcgacattca tcgtttcttg ttttgacact gcatcattgg cggataggaa acgatatttc
    20761 cgcaacattt cttccgcaag tctaatgcgc cttttctggc cgtctatctg ttgcaaaata
    20821 tggagtttct ggttttccaa acgttcgaca gttgctttaa ggctgcgcgt ttcattctcg
    20881 tgtatcagct tcagacgacc cagttcctgt tctgccaacg ttttcttcaa aaccgcttcc
    20941 gttttcaact gctgctgcac gctacctcct gcgccgaaac gtgaggtcga aagcgcaaat
    21001 agcttgtcgc cagccttgac cttttctcca tcttccacga atttcgctgt aattgtcccc
    21061 gtatccggtg catacaccct gattacgccc gatgcaggta aaatttgtcc ctccactgtt
    21121 gtctttcgcg tatagttacc gaatatcaaa aacaggataa tcagtaacgc agatatcgat
    21181 gcaaatgtcg tccataggga aaatgacaac ggtcgtgtca gaatcacttt acccgtcagg
    21241 ctggtttgcc gggcaatggc gacttcaggg cggaagaaag gttgcttggg tctattcata
    21301 aaattgaagt taagaaagtt tcagacgacc cctagagatc gtctgaacaa tgagaaatat
    21361 cagcagtaat ctgtaccgtc agtgtagccg tttcctgatt tatctgcttt tgttgcggga
    21421 gcagttaatc catgttcaat ctcaaagatt ggtcttccgt tataaggagg tgcattaacg
    21481 gcatcattta cccaatcacg agtcagattg tatacaccat ttgcaccata tagcaccgaa
    21541 aaaatttttc ggcaggaaat gaactgtcgc tgcagcagca ggtattgcaa caaaatcccc
    21601 ccatgtggga cctcctttgg ttgtggcagc attagctaca tttccagcta tattgtctgt
    21661 tacaggacct ccccctgaaa ccagcttcaa ttcatgaagt tgaagttcaa tcatttttat
    21721 actccttttc ttggttggta ttcctaaaaa ttcggctaac aaaaacatat ggcagatata
    21781 ttgaaaaaaa attcaaagta ccctgaataa aattcaaatt ccaactatat ttgttaatgt
    21841 agtcgagaag aaacatatct gataaagaat atagcaattg ataacaagct attactaata
    21901 ttacgaaaga tgtaaactgc ttccagtttt tcatagaatc cctcacaaaa tttccagaaa
    21961 atctaactct atcaactgat agatcaactt cctaacttct tcatgttttg cctgattgaa
    22021 gttaaccagt agatttttca acaataacgg ttcattctta ccgatgtgtt ctaacacttt
    22081 tttccccaac tcatctacgc ttatcttcat cccattccca atcaaatatc ccttttccaa
    22141 cgtatccaaa ttattggcat ttaatctcaa cctgacgtcg tctgaaagcg gagtagcgtt
    22201 gggattcgcg aactgttcga gatgaaaagc ggtatcggta cgttctttgc cgagaaagtc
    22261 ttcactgaag gcttcgtaat tgacggggtc ggcaatcatg gcggcaattt gtgcggcagt
    22321 atcgttgata cgcgtcctat cttgctccca gtctgagaaa ctgtggcgca gactttctat
    22381 ggtgggaaat ttcttcatta gccactcgag gtaattatag ccgtttggtg ggaaggtacc
    22441 gacagcgaag tggaaggttt cacagccgag cgggataggt ctgtgccacc aaccgcgtgg
    22501 gatgtagagg acatcacctg cttcaaggat aatatccata tcgatatgtt caggaatgga
    22561 aatatcagta tctttagtct gttgcatata caatggcata gggaaatcag gggcagtaag
    22621 ttgccaacgt ttcttgccga aaagctggac ggcatacata tctcgggcgt cccaatggtt
    22681 tttataagat tcgtcgctgc caaaagcaag atatccacta acaatagtat gtgcgccggc
    22741 aaagcgggcg acttgacggg cgatatggtc tgaaaacggc tcgttgttaa tatggttata
    22801 gactaacgac gcaccgttct tcatatgttc gtagataacg gatttaataa aacggtagcg
    22861 agttttgccc aaatcgtcga aactttcgac gtattcttct ttaggaacga ttgcgccttt
    22921 tttacgcaga tgaaacagcg gtgcggttgg gtctgctcgt tggtatatct cgttgatata
    22981 tttacaagat gcggcttcga gattccgaac cgctccttta aagagcttgg gcttttgata
    23041 cagataagtc tgtcggaacg ttttaggact aatgccgaag tcgagatgga tgcccattac
    23101 ttccccttac tcagaaaata tttaaaattt ataatgttac atatagttac aaatattaga
    23161 gttttttgtg tgtgcgtcaa ggaattgttg acaattttag ttaaaaattt gtctccaaac
    23221 ggaaaaaagc ggtttgtttt ttgttgttaa catttttatt gtaattaaat aaaaggtcgt
    23281 ctgaaaacgg ttttcagacg accttttgct ataatcgggc ttcatcgccc cgttcggttt
    23341 ggaaccttat gaaaaccctc gtcctcctcc tgctttcctt ctccacgacc accgctttcg
    23401 ccgcatacgg tttgggtttg gggcaggcac cgaaatatcc tgccggcttt cgcgcctacg
    23461 gttatgttta ttccggacgg cagggctggg ttttaaaaac agaggcggat gccattaaat
    23521 tagacacgct tttcaaacgc tttgtgtacc gtccttccgc cgccaatcaa aaccccgtcg
    23581 gacagcgttc ggacggcata cccgccaacc acacaaagga aaaaccatga gcaaaaaaat
    23641 caaagccggc attgtcggcg cgacgggcta cacgggcgtg gaactgctgc gcctgcttgc
    23701 cgcccatcca gatgtcgaag tcgccgccgt aaccagccgc agcgaagcgg gaaccgcagt
    23761 tgccgattac tttccaagtc tgcgcggcgt gtacggcctc gccttccaaa cgcccgacga
    23821 ggcaggtttg gaacaatgcg acatcgtctt cttcgccacg cccaacggca tcgccatgaa
    23881 agacgcaccg cgcctgattg aacagggcgt gcgcgtcatc gacctttccg ccgacttccg
    23941 catacgggac attccgacct gggaacactg gtacggcatg acccacgccg cccccggcct
    24001 cgtttcccaa gccgtgtacg gattgagcga actcaaccgc gaagccgtcg cacaggcgcg
    24061 cctcgtcgcc aaccccggct gctacccgac ctgcgtatcc ctgccgctcg tgccgctgtt
    24121 gcggcaatgc cgtctgaagc ccggtatgcc gctgattgcc gactgcaaat ccggcgtgtc
    24181 cggcgcgggc aggaaaggca atgtcggttc gctgttgtgc gaagccggcg acaacttcaa
    24241 agcctacggc acagccggac accgccacct gcccgaaatc aggcagacca tcgccgggct
    24301 tcaggacggc atcgccgaag gattcgtgtt cacgccgcac ctcgcgccga tgatacgcgg
    24361 tatgcacgcc accgtttacc tccacctttc agacggcagc gaccccgaaa ccgtcctgcg
    24421 cgactactac cgcgacagcc cgttcatgga catcctgccc gccggttcga cacccgaaac
    24481 ccgcagcgtg cgcggcgcaa acctctgccg catcagcatc cgtcaggcgg cgcaatccga
    24541 tgtgtgggtc gtcctttccg tcatcgacaa cctcgtcaaa ggcgcggcgg gtcaggcagt
    24601 ccaaaatatg aacattatgt tcggactgga ggaaacacac ggcttgggcg gcatccccct
    24661 gctcccctga agcgcaaacg gcaaaccgca ggcatcgtgc ctgcggtttt ttgatgccgt
    24721 ctgaaagcgg cggttttttt gggttcggac ggcttttggc tcatcattca gcgaaaacaa
    24781 aaatctaaaa taccgtcatt cccgcaaaag cgggaatcta gtttatccgg cttcagcaat
    24841 ttccgacaca tttccacacg cttcgattcc gtcatttctc cggtttcaat cgtttccgat
    24901 aacaccgtgg tttttcattt ctagattccc gcctgcgcgg gaatgacggc ggggggcttg
    24961 ccgttttccc gataagcctc tgcggcttct cattgccgga tttccgcccg cttgggaata
    25021 acgggattta aaatcatgaa aatgtgtcaa aaataatata gtggattaac aaaaaccggt
    25081 acggcgttgc cccgccttag ctcaaagaga acgattctct aaggtgctga agcaccaagt
    25141 gaatcggttc cgtactattt gtactgcctg cggcttcgcc gccttgtcct gatttttgtt
    25201 aatccactat aaaaaatcag atttccgtta cacttttttc taatatttca gacggcattt
    25261 tgctcacaca cccaaataac cttccctgcc ggaaagccac cttgccaaat gcgcttcgac
    25321 gatttcaggg ttttgttcaa tcagcatcgg ggcggtttcg cgcgcttgtt ccaaaaggtg
    25381 taagtcttct tcgagcttgg cgaagcgcag cataggcacg ccgctttggc gcgcgccgag
    25441 aaattcgccg gggccgcgga tattgaggtc ttggcgggcg atttcgaagc cgtcggtgtg
    25501 ttcgtagatg actttcagcc gcgctttggc gagttcgccc aagggttcgg caaacaggag
    25561 gacgcacacg ctttcccccg cgccgcgccc tacccgtccg cgtaattggt gaagctgcgc
    25621 caagcccatg cgctcggcgt gttcgatgac catcagggcg gcattgggca catctacgcc
    25681 gacttcgata acggtggtgg cgaccaagac gttcagacgg cctgcggcaa attccgccat
    25741 gacttcggcc ttttcggcgg ccttcatgcg cccgtgtacc agtccgatat tgggttcggg
    25801 caatgccgtc tgaagccggg cgagggtttc ggcggcggtt tgcagttgca gggtttcgct
    25861 ttcttcaatc aatgggcaga cccaatatgc ctgccgccct tttcggcaag tgccgaggac
    25921 gaagccttcg acttcggcgc ggcggacgtt gttgacgagg cgcgttttaa tcggagtgcg
    25981 cccgggcggc aattcgtcga tgacggatac gtccaagtca gcgaagaagc tcatggcgag
    26041 cgtgcgcggg atgggtgtgg cggacatcat cagctgatgg acttcgcgcc ctttgttttt
    26101 gagtgcgagg cgttgtgcaa cgccgaaacg gtgctgttcg tccacaatgc tcaagcccaa
    26161 attgtgaaac gccacgccgt ctgaaaacag ggcgtgcgtg ccgacggcga ttttgacgct
    26221 gccgtcggcg agtttggctt tggcttcgtc tttggctttt ttgcgcaaac tgccaaaaag
    26281 gcagacaact tcaatgccca aaggttcgag ccattgtttg aacttgatga agtgctgttc
    26341 ggcaaggatt tcagtgggcg ccattacagc cacttgcgcg cctgattcga tggcggtcag
    26401 cgcggataag gcggcgacga tggttttgcc gctgccgaca tcgccttgca gcaggcggtg
    26461 catggggtgg gtttgcgcca tatcgcggca gatttcggaa acaacttttt cttgtgcatc
    26521 ggtcagggca aacggcaggg cttggcgcag ggcttgggtc aatgtgccgt cgccgcgcaa
    26581 tgccgccgcc gtgccgctga cgcgcttctg ccgcgccaaa cgcatggaca actgttgcgc
    26641 caaaagttcg tcgaatttga gccgctgcca cgccggcagc gtgccgtctg aaagttgatg
    26701 aatagtgaaa ctcggcggcg gcgaatgcaa aaggcgcagg ctttcggcaa ggcgcggcag
    26761 cttcagacgg cctaataaag catcaggcaa ggtgtcgtgc agcggcgtaa cgtccaacgc
    26821 cgtctgaatg atgcggcgca aagtgggctg gttcaaaccg tttacggtcg ggtaaaccgg
    26881 cgtgaggctt tccgccaaac cgccgccctc ggcatcgcgg attttgggat gaatcatctc
    26941 gtcgccgtaa aatccgtgtt tgatttcgcc cacggcgcgg atgcgtttgc cgaccgccgt
    27001 ctgcttctga tggctggcgt aaaagtggat gaagcgcaga aaaaggacgc tgccggaacc
    27061 gtcggcaatt tgaacaatca gctgcttgcg cggtttgaac gttacttcct gatggataac
    27121 ctcgccctcg acctgacacg gcacgccaat cggcgcgtcc ttaatcggca taatgtgcgt
    27181 ctcgtcctcg taacgcagcg gcaggtgcaa caccaaatcc cacgcggtat ggaggttgag
    27241 tttgtcgagc ttcttggcgg aaacatcggt gattttgagc tgtttctggg tttcgggcga
    27301 catcataggc agattccttt ggacgcgcct attttatccg aaaacaaaaa tgccgtctga
    27361 aacggattca gacggcatcg gcaggcagga atcaagcccc ggcggcttcg gcttcctgct
    27421 gttgttggta aatcgcttca aaattaatcg gcgcaagcag gacgggcggg aagccggcgc
    27481 gcgttaccgt accggaaacc gcttcgcgcg cgtaagggaa gaggatgttc ggacacgcca
    27541 cgcccaacag cagctgcaca tcttcttcgg ggatgttttc cagacggaaa ataccgcttt
    27601 gggttacttc gttcaaaaac atcgtgcgct cgttatccaa tttggcggtt acggtaacgg
    27661 ttacatccac gtcgtagtag ccgtcttcca gcttttggct gccggtggaa acgcgcatat
    27721 ccacttcggg atcgccctgt tccaaaaaga tttgcggcgc gtgcggcact tccaaagaca
    27781 agtctttgac atacagtcgc tcgatgctga atacgggttg cagttcttcg ctcattttgt
    27841 tttcctagtt gtgggttaag ggttcagcag tccgtccagc ccgccttcct gctgaaggcg
    27901 gtagaggtcg gtaaatcctc cgacgtgcgt ttcgccgatg aaaatctgcg gcacgctgcg
    27961 ctgtcccgaa agccgctgca tttcggcaaa ggcttcgggg cttgcatcga cacggatttc
    28021 gtcgatatgt ccgacacctg ccgcgtgcag cagccttttc gccatcgcgc agtaggggca
    28081 aaacggacct gtgtacatgg taacggtctg catattggtt ttccgaaagt tttgcaatga
    28141 taatcaatat aggggcaatc gctccgtttg gcaagtgcgg aacagatgca cgttcagacg
    28201 gcatgtgcgg agtgtgtcaa agtttctttt ttaaagtatg ataggcattg tgaaaaatat
    28261 ttttgcaccc gctctgcgcg gcggggcgga acggatgcaa aaatattttt attacatttt
    28321 caggaaaaac catgttgtca ggactcccca tccccaaaga catcgcgcgc ccgcccgaaa
    28381 cgatattggt caacatcacg ccgcaggaaa cgcgcgtggc ggtgttggag gaaaacaata
    28441 tctgcgagct gcacatcgag cgcaacagcg ggcacagcct ggtcggcaat atctatctgg
    28501 gcgtggtgcg ccgcgtactg cccgggatgc agagcgcgtt tatcgacatc ggcttggaac
    28561 gcgcggcgtt tttacacatc gtcgatgtcc tcgaacaacg ccgcaacccc gaagaaaccc
    28621 agcgcatcga acatatgctg tttgaagggc agtcggtttt ggtgcaggtc atcaaagacc
    28681 cgatcaacac caaaggcgcg cggctttcca cccaaatctc gctggcgggg cgtttcctcg
    28741 tccaccttcc gcaagaagac cacatcggcg tgtcccaacg catcgaagac gatgccgaac
    28801 gcagcagcct gcgcgaacgc ctcgacaagc tcctgccgga aaatgcctgc cggggctaca
    28861 tcatccgcac caacgccgaa aacgccaccg acgaacagct ccagtccgac atcgactacc
    28921 tgaccaaagt gtgggaacac atccaagaac aggcgaaaat ccggccgccg gaaaccctgc
    28981 tttatcagga tttgccttta agcctgcgcg tgttgcgcga tatggtcggc tgcgacacgc
    29041 agaaaatcct cgtcgattcc accgtaaacc acgggcgcat gacgcgtttt gccgaacaat
    29101 acgtccacgg cgcattgggc aggatagaat tgttcaaagg cgaacgcccg ctgtttgaaa
    29161 cccacaacat cgaacaggaa atcagccgcg ccctgcaacc gcgcgtcaac ctcaacttcg
    29221 gcagctacct gattatcgag tccaccgaag ccatgaccac catcgatgtc aacaccggcg
    29281 gcttcgtcgg cgcgcgcaat ttcgacgaaa ccatcttccg caccaacctc gaagcctgcc
    29341 acaccatcgc ccgcgaatta aggctgcgca accttggcgg catcatcatc atcgacttca
    29401 tcgatatggc gcaggaaagc caccgcgaag ccgtgttgca ggagcttgcc aaagccctcg
    29461 ccttcgaccg tacccgcgtt accctgcacg gttttaccag cctggggctg gtcgagctga
    29521 cgcgcaaacg ctcgcgcgaa aacttaaacc aagtcctctg cgaaccctgc ccttcctgcc
    29581 aaggcagggg ccgcctgaaa acgccgcaaa ccgtatgcta cgaaatccag cgcgaaatcg
    29641 tccgcgaagc gcgccgttac gatgccgaaa gtttccgcat cctcgccgcc cccaacgtca
    29701 tcgatttgtt tttggacgaa gaatcgcaat ccttggcaat gctgatagat ttcatcggca
    29761 aaccgatttc tttggcggtc gaaaccgctt acacgcagga acaatacgat attgtgctga
    29821 tgtaaaaaat gccgtctgaa gccttcagac ggcatctgtc tatttcaggg tttccttgtc
    29881 caacagcgcg cgtatcaaca gcccgtgtcc gaaacgtcgg ctgtcggtca gttccaaata
    29941 tccgccgtat tttttggcca gcgtgtcggc aatcgacagc cccagccccg ccccctgctg
    30001 ctccgttccc aaaatacggt aaaacggatc gaggacgcgg gcgcattcgg attcgggaat
    30061 gccgttcccg ttatcctcca cccacacggc gagatatttc ccttcgtccg tgaaacccaa
    30121 atctatcctg ccctcgggcg gcgtgtaaca taccgcgttg tcggcaaagg ttttaaccag
    30181 cgtatagatt tccgtttcgt cggcagacac ttcgacatcg cctccgaccg ccacaccgat
    30241 gtcctgacgt ttttccaaaa ccagcggcat cagttcctgc aacacttggc ggaaacggct
    30301 ttgcagaccg aatgtcgttt tcgccaaggg gggtttcgtc cgactgcgaa cgcgccagtg
    30361 ccaaaagctg ttcgagcagg tgtttgttgc gcctgatgct ctgctgcaaa acggcggact
    30421 gccgccccgc atcgggtggg agcggcatat tgttgagccg ttccgcctga agggaaaggg
    30481 cagtcatcgg cgtacgcaat tcgtgtgccg cgtcggcgac aaaccgctga cggcggcgta
    30541 tatcttcatc aacacgtttc aaaagcagat tgatggcggt tacgaaccct ctgatttcac
    30601 tgggaatatt gtacacactc aaagcaggca ggccgttgat tcggcgttgt tcgagacttt
    30661 gcgacaattt gcggacgggg cgcatggctt tgtgcgtaat ccacacagtc agcagtatca
    30721 tcagcggcag tgccgccaac aggggcaaca cgctttgccg tgccgcatcc tccgccaaat
    30781 cttcacggta ttcgttttcc tgcatgacgg caatccgtcc ctgttcggtc gtgcggatat
    30841 aggcgcggta ataatcgtcg tccccgtccg cccgaagcgt gtgcagaccg tccgccagat
    30901 gcgcgggcag gctgacaaca gggtcttcct gctgcggcat ctgtaccaaa atacgcgtat
    30961 cgccgtcgcc ttcgggcaaa gtttcggatt tggaatcagg gccgacatac aatgccgcct
    31021 gacggagcag gtcgtcctgc aacgcttccg tttcgtggaa ggtttcgtag taggaaaaca
    31081 tacctgcaag cattgccagc ggaacaaaca tccaaatcag ggcaaggctg attctgacct
    31141 gaagcgaatg ttttaaaatt ttaaaaaaac ggtccggcat cttcgttcct gttcagacgg
    31201 catcttgacg cggcatcagc cagccgacac cgcgcacatt ttgtatgctt tccttaccca
    31261 atttcttgca cagcccgtga atcagaaaat ccaccgcatt gctttcgact tccccgcccc
    31321 aaccgtaaac cttgtcctcc gaatccgagc gcgacaaaat cacacccggc ctcgccagca
    31381 aagcctgcaa taccgaaaac tccttgttgc tcaatgccac ctgcctcccc tcggcaataa
    31441 tttctacctg atacgttgca ggattgaggg ataacgcacc atttgacaga cacgcctgcg
    31501 cctgtccgct cccgcgccgc aacactgccc gcatccgcgc cttaaactcc gccatatcga
    31561 acggcttgac gatatagtca tccgcaccgc cgtccaaacc gttcagccgg ctgtacaaat
    31621 catcccgcgc cgtaacgatc agcacgggga cggtacagcc tgccgcgcgt atttccgaca
    31681 aaacatccaa accgtcccgc ccaggcaaac ccaaatccag cagcatcaag tcataaggct
    31741 gggcagcggc agccgccgca acctgcgcgc cgtttttgac ccaatccacc gcatagccgc
    31801 cgtctttcaa acttgccgac accgcttccg caatcatcgc atcgtcttcc accagcaaaa
    31861 cacgcatcaa ctttcccttc aaaataaacc gtgcctattc taacacccca aaattagccg
    31921 caatttagcg gcctttacgc ttgccggtat ttttcaaaac tgcggcacaa aaaaaccgcg
    31981 ccggcaaccg ccttcagacg gcattggggc gcgattgcaa cacacggaca ggcaggcaaa
    32041 gcctgcgaca gatcacagga acgattcggg cttcaggcgg cttcgccgtt tacggcagag
    32101 gcacgattcc tgccgctatc gaactgacca atatcgccag cgacaaaccc cacgcccaga
    32161 aaaacgaata acggatgtgt ttgcccatcg acaatttcgc caaacccaaa cccatccaca
    32221 aagccggcga aagcggcgta acaaaagtac cgacgatact gccgatcaac atcgcatagc
    32281 ctgccgcttc gggtgcaacg cccgcctgcg aggtaatctg ttccacaatc ggaaacagtc
    32341 cgaaataata agcgtccgta ctcaaaacca actcaagcgg aatacccaac acaccgatgg
    32401 caatatgcag ataaggcagc aacgcgtccg gcaggatatg cacaaggtct ttggaaatcg
    32461 cgtccaacat ccccgcgccc ttcaaaatcc ccaaaaacgt acctgccgcc aaaataatgg
    32521 acgccatcat caccgcgccg ccggcgtggg cataaatccg ctccatctgt tcctgcgggc
    32581 tgcggtaatt caaaagcaac gccgccgttg cagccagcat aaatacataa cccggcggga
    32641 agatgcccga aaaaagcagg ctcatcgccg ccaaaaacag caggacattc caccaaaaca
    32701 gtttcggacg cgccaatttt tgttcttctt ccgacaaagg cgccggcttt atcaaatccg
    32761 ccacggcggg caacgcgccc aactcccgga caatccgcct tttttcacgc acacccaaaa
    32821 acagggacag cacaaggata aacaccacac cgataatttg caccgtcaac aaaggtttat
    32881 acaattcgcc cacatctgcg cccaacacgc ttgcaacccg cccgatcggc ccgccccgcg
    32941 gcaaaaggtt gattagcccc gcgctggaag tcagcagcaa aaacagcagg taaggattca
    33001 tatgcagacg cttgtaaagc ggcaaaaggg cggggacgac cgataaaaac gtcgtcgcgc
    33061 ccgccccgtc caactgtgcc accaccgaca ccaagaccgt ccccacactc actgccacga
    33121 tattaccccg agtcagctta atcaaaccgc ctatcatcgg acggaacagc cccacatcgt
    33181 tcatgattcc aaaaaacaaa atggaaaaca taaacataat cacaatctgc gtcaccgatt
    33241 tcgtgccgcc cgaataaaat tcttttaatt gggatacatc aaaccccgcc agcaacgccc
    33301 caatcagcgg caccaagatt aatgcgatga tgggcgacac tttttccgtc agcaacagcc
    33361 atacgatgac cccgataatc agcaatccga taaacgtcag cattatttct ctttatttta
    33421 ttttgaacat aaaaccgccc gtgcaggcaa aaccgccaac atatgcggga taagccttgc
    33481 attccattca cgcctgccga catttctgca tccgtccgtc atttcacggc ggcaaccgat
    33541 acggaacaac cggtatcggg acggcgcggg ggcatccgtc ccggtgcgcc gattcaaacg
    33601 aaactgcccc ctatcattgc ggagcgcggg gcgtgccgta cacgcgggat tttaaacacg
    33661 ccgatcattt gccgcgcatt atccggcaaa cggcaaacct tgacgctgcc cggcccatat
    33721 aaaaaagccg caaaacccga accggttttg cggcttttcg atttggtttg tttatttggc
    33781 atttctttca atcaaaccga caaactgatt gatatatgac tgaacaaaat cccttgccga
    33841 gtcaatcaac ttaccgtttt catcaaacag cgtcggcgaa ttgcccaaaa atacttccgg
    33901 ctgtccggtt acgggcatat caaaatacga cagggcaagg cgcaggtttt tttgggaact
    33961 gtaaccgccc atcttgccga cggaatggct gatgatgccg gccggtttgt ttttccaagc
    34021 cacgtcggca ttcggtttcg agccgatgtc caccgcattt ttcaaacagg cgggaatggt
    34081 gcggttgttt tcggacgtaa cgaacaaaat gccgtccgaa gccttaatcg tttcgcggaa
    34141 agccgtgtag ctttcgggca gcggcacatc ttccacctca gggtcgtcat aatcgaaatt
    34201 gtaaagcggc agatgtccga tttcgacgat ttccgcctgc cagccttcgg ggaacatctc
    34261 cgccgcattc aatgccactt tgcgcgcaaa cgaagcacgg cgcaggctgc ccaccaaaat
    34321 actgattttc ttagccacaa tcattcctcc tgaatattaa gtttgtgcgt ttcaatcatt
    34381 tcataatggt aacgattatt atatatgtga tttcccctgc aaacaagccg gtccgccgcc
    34441 ccggcgttcc cacttatccg gctttgcctt ataattgctt tttttatgta acagattcac
    34501 ctatgaattt ccccaaaaca gcggcctccc tgctgctgct tctcgcctcc ctcgccgcac
    34561 acgcgctcga taccggccgc attccgcaaa acgaaatcgc tgtatatgtc caagagcttg
    34621 acagcggaaa agtcatcatt gaccaccgtg ccggcatacc cgtcaatccc gcgtccacga
    34681 tgaagctcgt taccgcgttt gccgccttca aaaccttcgg cagcaattac cgctgggcga
    34741 ccgagtttaa aagcaacggt acggtaaacg acggcacgct tgacggaaac ctgtattggg
    34801 cgggcagcgg cgaccccgtt ttcaatcagg aaaacctgct tgccgtccaa cgccagttgc
    34861 gcgacaaagg catccgcaat atcacggggc gcctgatgct cgaccacagc ctgtggggcg
    34921 aagtcggcag tcccgaccat tttgaagccg acagcggttc gccgtttatg acgcccccaa
    34981 atccgactat gctgtctgcc ggtatggtta tggtgcgcgc cgaacgcaat gccgccggca
    35041 gtaccgacat cctcaccgat ccgcctttgc cgcatatttt tgcccaaaac aacttgaaaa
    35101 ttaccgcctc ccaagctgcc tgcccttcgg tcaaaaaact gatgcgcgca tctttttcgg
    35161 gcaatacgct gaaattgcgc ggcaatattc ccgaaagctg tttgggcaag cctgtcggtg
    35221 tccggatgtt cgcgcttgac gaactgatcc gccaaagttt taccaaccgc tggctgctcg
    35281 gcggcggacg gatttcagac ggcatcggca tagccgacac accggaaggc gcgcagacgc
    35341 ttgccgttgc acacgccaaa ccgatgaagg aaattttgac ggacatgaac aagcgttcgg
    35401 acaatctgat tgcgcgttcc gtcttcctca aactcggcgg cgacggcaaa ctgcccgccg
    35461 tttccgaaca ggcggcgtct gccgtccggc gagaacttgc cgtatcgggc atcgatgttg
    35521 cggatttggt tttggaaaac ggttcgggtc tgtccagaaa agaaagggta acggcgagaa
    35581 tgatggcgca aatgttggaa acggcttatt tcagcccgtt tgcacaagat ttcatcgaca
    35641 cgctgcccat cgccggcaca gacgggactt tacgcaaccg cttcaaacaa agcggcgggc
    35701 tgttgcgctt aaaaaccggc acgctcaaca atgtccgcgc ccttgcaggt tattggctgg
    35761 gcgacaaacc gatggcggtg gtcgtcatca tcaacagcgg ccgcgccgtt tccctgctgc
    35821 ccgacttgga caacttcgtt gccaaaaaca tcatctccgg cggcgacggc tggctggatg
    35881 cgaaactgat gtgcaaagaa cgccgcgcct gaatcgggaa aatataaaaa tgccgtctga
    35941 actgttcgga cggcattttt gattttcaaa ccggaattac agccccgctg ccgctttcaa
    36001 tgaggccgct ttgtcggtgc gctcccaagt aaactcgggt tcttcgcggc cgaaatgtcc
    36061 gtaggcggca gattttccgt aaatcgggcg caagaggtcg agcatttgga cgatgccttt
    36121 gggacgcagg tcgaaatgtt cgcaaaccag cgcaatcagt ttttcctcgc tgattttacc
    36181 cgtaccgaaa gtatcgatgg aaatcgaagt cggttcggca acgccgatgg cgtaggaaac
    36241 ttggatttgg cattgggttg ccaagcctgc ggcgacgatg tttttcgcca cataacggca
    36301 ggcgtaagcg gcggaacggt ccactttgga cgggtctttg ccggagaatg cgccgccgcc
    36361 gtgcggagcc gcgccgccgt aggtatcgac aatgattttg cggccggtca aaccgcagtc
    36421 gccttgcggg ccgccgatga cgaagcggcc ggtcgggttg atcaggtatt ttgtttcgtc
    36481 ggtcagcagt tcgggcggca aaacgggctt gataatctgc tcaatcacgg ctttactcag
    36541 ttcttcatgg ctgatggcag gatcgtgctg ggtggacagg acgacggtat caatgcgttt
    36601 caccttgccg gtttcgctgt cgtaaaccac ggtcagttgg gcttttgcgt cagggcgcag
    36661 ccaaggcagg cggccgtctt tgcgcaattc gctttgacgc tgcatcaggc ggtggctgta
    36721 atagatggca aacggcatca gggtcggggt ttcgtcgcag gcgtagccga acatcaagcc
    36781 ttggtcgccc gcgccttggt tcaggtcgat gccttcgcct tcgttcacgc cttgggcgat
    36841 gtcgggggat tgttggtcgt agtacacgcc gaccgcgcag ccgttggcgt caaagcccgg
    36901 ctcggaggag ttgtagccga tgcgtttgat ggtttcgcgt gcgactttga tgtagtccac
    36961 ttgggcggtg gtggtgattt cgcccgccaa tacgcacaag cctgtgttga ccaaagtttc
    37021 cgccgcgaca cgcgctttgg ggtcttgcgc caagatggca tccaaaatcg catcggatac
    37081 ttggtcggct actttgtccg gatggccttc ggaaaccgat tcggaagtaa acagatattc
    37141 gctcattgaa attttccttg aaaaacaaac catcttcttc agacagcatg ttgtatgaac
    37201 ataatgtcga cagcgggaaa tatagcaaaa tttccctatt cgtgccattc agttgagaaa
    37261 tattcctatt taaacggcgc tttggaatct ctgtccgtac gtttcgtaca ggcaaaaaaa
    37321 ttcccgcatc aagcgggttt ggattgctcc ggcgagccac atcggttttt caaccgtcca
    37381 ccttaccttt ccgtttgaaa agcaggttgg catggaattc ccaactctta atgcagcgcg
    37441 catcgtagca gaaaaggcat attgccgcaa tacttccctt tttcagacgg catgtttcgt
    37501 ttacaattca ggctgtttcc ccctttgcga accgccatgc acatcctgtt gaccgccctg
    37561 ctcaaatgcc tctccctgct gtcgctttcc tgtctgcaca cgctgggaaa ccggctcgga
    37621 catctggcgt tttacctttt aaaggaagac cgcgcgcgca tcgtcgccaa tatgcggcag
    37681 gcgggtttga accccgacac gcagacggtc aaagccgttt ttgcggaaac ggcaaaatgc
    37741 ggtttggaac ttgcccccgc gtttttcaaa aaaccggaag acatcgaaac aatgttcaaa
    37801 gcggtacacg gctgggaaca cgtgcagcag gctttggaca agggcgaagg gctgctgttc
    37861 atcacgccgc acatcggcag ctacgatttg ggcggacgct acatcagcca gcagcttccg
    37921 ttccacctga ccgccatgta caagccgccg aaaatcaaag cgatagacaa aatcatgcag
    37981 gcgggcaggg tgcgcggcaa aggcaaaacc gcgcccaccg gcatacaagg ggtcaaacaa
    38041 atcatcaagg ccctgcgcgc gggcgaggca accatcatcc tgcccgacca cgtcccttct
    38101 ccgcaggaag gcggcggcgt gtgggcggat tttttcggca aacctgcata caccatgaca
    38161 ctggcggcaa aattggcaca cgtcaaaggc gtgaaaaccc tgtttttctg ctgcgaacgc
    38221 ctgcccgacg gacaaggctt cgtgttgcac atccgccccg tccaagggga attgaacggc
    38281 aacaaagccc acgatgccgc cgtgttcaac cgcaataccg aatattggat acgccgtttt
    38341 ccgacgcagt atctgtttat gtacaaccgc tataaaacgc cgtaacgaaa ataaaaatgc
    38401 cgtctgaaca ggtttcagac ggcatcttgt catttgacga tttcggacaa cgaccaacgc
    38461 ggacggacgt tgaacgcacc gacctccctg cccttgccca ggtgcatcgc accggcaaag
    38521 gcaatcatgg caccgttgtc cgtgcagtat gccatcggcg ggaaaaacac gctgactttt
    38581 tcggacggat gtttcggctt gcctttgggg gtcgggattt gtaccgtcat gttgccgaaa
    38641 gtttcacgga gtttgcggtt tgcaccgacc ccgccggcga ccactacggt tctgaaccct
    38701 gtctgcaaca gggctttttt cacttttgcc tccaacactt ctactaccgc atcttgaaac
    38761 gcacggcaaa tattgttgcg ggtttgctcg ggtatggttt ccgaaccgtt tgcctcacgc
    38821 actttttcaa cggcagtcaa aacagcggtt ttcaatcccg aaaaactcat ctgcaaatcg
    38881 tcggaatgaa tcatcgggcg cggaaaaaca aacgcttcgg gcctgcccga ttccgcaagt
    38941 tccgacagtt tcgcaccgcc cggatacggc aaacccagca gtttcgccgt tttgtcgaat
    39001 gcctcgcccg ccgcatcatc gacgctctcg cccaaaagct cgtagtcgcc tatgcccctg
    39061 accgccataa tctgcgtatg cccgcccgaa accaacagcg cgacaaaagg aaagtcgggt
    39121 tttccctccg ccaacagcgg cgacagcaga tgtccttcca aatgatggac ggggatgacg
    39181 ggcttatcca acgctaaagc cagcgcgttg gcgtagctcg aacccgccag cagcgcaccg
    39241 cccaaaccgg gcccctgcgt aaaggcaacc gcgtcaatat cgccatacga tgcgcctgcc
    39301 tgcgccaaac agccttcagt caacggaaca aggcggcgga tatggtcgcg gctcgccaat
    39361 tccggcacga ccccgccgta ttccgcgtgc attgccattt gagtgtgcag gcagtgcgac
    39421 cgcaatcctc gttcggtgtc gtacagcgca acgccggttt cgtcgcaaga agattcgatt
    39481 cctaatacca acatggtctg atgccgttaa aaactgaaaa acgtattcta gcgaatttcg
    39541 gcacgactgc cgtatcccca aaacggaaca tgccgtctga agcccttcag acggcatcgt
    39601 cgcaccgtat caaagcgttc cgtaagaatg cagcccgctc aaaaacatat tcacgccgat
    39661 aaaggcgaat gcggttacga acaaaccgat aatcgcccac cacgccagca ctttgccgcg
    39721 ccagcccgcc accagccgca aatgcagcca cacggcgtaa ttcaaccaaa cgatgaacgc
    39781 ccaagtctct ttcggatccc agctccaata gcgtccccaa gcgtccgccg cccacaacgc
    39841 gcccaaaacg gtggcaatgg taaagaacaa aaagccgacg gcaatcgcct tatacatcac
    39901 ctcgtcgatc aatgccgacg gcggcagcca cagttttccg cctttttctt ccgcacgcag
    39961 ggaaaccagt tcggcaatac cgagcatcgc ggaaatgcag aacgcgccgt aaccgataaa
    40021 gtttgccgga acgtggattt tcatccacca ggactggagc gcgggaatca gcggctggat
    40081 ggtatgcgcc tcacgcgaca cgctgtacca caacacaaag ccgaccacga ccgccataaa
    40141 gccgaacacg aagccgccca atttctgcac ggcaaattta ccctcgtaat acaaatacat
    40201 cagcgcggta atgaccaaaa acagaatgaa cacttcataa aggttggaca cggggatatg
    40261 ccctgcatcg ggacggagca gatagctttc gtgccaacgt accagcagac cggtaaagcc
    40321 tgctacggca gacacccatg caaacacggt tcccataccc aacagcgtgt tggtcggcac
    40381 atttttcacg cttgccaaaa ccgcgcccga aatataggcg aacagggcga agaagacaaa
    40441 agcacactgc cacatgatcg ccgattggct gctgaggaaa taccgcaaca ggaaaatctc
    40501 tgccgattta atgttgccgc catacagacc gacggcggca taggcaagca atacgcttaa
    40561 aggaacaaac cagcgcatcg gtttgaaaaa ccaacccaaa aacacggcaa taccggcact
    40621 cgcccacaac atgaccgttt cgtaaatgtc catatgcata ccggaacggg tctgcacgaa
    40681 aaccgtagcc gcaaaaacca gcacggcaaa tacccaatcc caaagattca gattgcggat
    40741 caaagatttc tgaatcagca gctcgtgttc cggaagggtt ttatagtgtt cagtcatgat
    40801 tcaagtcctt gccgagccgt tgcaggctct cgacgtgttt tggaaattcc ttctgcaaat
    40861 cccgttcgct gcgggccgaa gacatagcaa aacggatttt gccgtctgaa aacaataccc
    40921 acgcccgttt ttcgcgcaca taaaacataa ataccgtacc caaaaccaac aataccgagc
    40981 cgagatagac caaaagcgca cccggcgaac gggtcatctg caaacctgag gaacgcacct
    41041 cggaaaaccc gtcaagctgg agcagcatgg gcgcgggata ttccgtcagc cccgtatagg
    41101 catccatact gtgcagcagg aaacggttcc gcgcttcatc ctgccgccat tcgggcaagc
    41161 cgtaccggcg tatggtttca tccaaagcag cgttcatcac gccgtaaagc atttcgtaga
    41221 aatagccctg catcttatcc tgctgccctt tcgggatatt ggacgtaata aattcgtcca
    41281 atcccaaata gcctttttgc gcaaagatat tcagcgtgtt ttccgcagcc agcatgaatt
    41341 gttcgcggat ttcggcaggt gcgtctttgg ttgcgtcggc aaccagacgt ttgcgccctt
    41401 ccccatcttt caaaaactca cgcaatgcca taaaggtgtc cgctttcaac tgcttgtcca
    41461 aggggatacg cagccagcgg tattgctgct gcaagccgct gcgcgtgccg gtcagccaaa
    41521 aataatcttt gtcctgcaaa atcggcagca tatagttttt atattcgacc gcctgccctg
    41581 ccgcatcacg gatgcggtac acgatggaag ggccgatatt ggtgtatttt ttaccttcct
    41641 gagtaacggc gcggacatcg ttcagagtgg atttcaggct tttttcccgt tccgcaccct
    41701 cgctcatgtc ctccacattc atagaagtga actgatcgaa ctcaagacga tatttgtgtt
    41761 tgccgatttc caacggaaac tggtgtatgg aggttgcctt caacacgaca ggttcgcgcg
    41821 aagcatccct caaattccac gccttgaatg tcaaatccga accgccgtcg gcaaaactcg
    41881 cctgataaat cgtgatgccg tgcaaggtca aaggatggtt cacgcggatg gtgcgctcga
    41941 gtttctcacc ggttgccttg tccgttactt caatatcgct ggcaaaatcg cgcggcatac
    42001 ccgtattgta aaaatcgata tggaattttt tcagtttgac ttcaaaaggc aagtcctgaa
    42061 ccaacatccc gttgtcggca ttcaggaaaa ccacatccgc actttgcccc tcggaaatat
    42121 tgacgttgcc cctaaatgag agattggacg cacccaaaat actttcgggc ttgaaatcct
    42181 tggcataaac cgcctgattg tccggaacaa tccgaccggc cagcataccc agcttcagca
    42241 gcaggttact gtctatcaac ccgcccaggc aaatgacaat caaagcaaca tgggcaaaga
    42301 tatagcccca tttgttcatc gtgccttttt tggcggcaat cagaaccgac ccgtcctcac
    42361 ggctgacggt ttttccctga aaaccccgca cctccagata acgtttggca acttcggggg
    42421 caatttttac atccaacagc gaagaatggc gcatcgccgc cagagatttt tctttaacct
    42481 tttcccggaa agacttcatt tcgcgccaaa acggcggaac gttacggatt aaacacaaac
    42541 tggtagaaac caccagaaac atcatgataa cgacaaacca tgccgaagca tagacatcgt
    42601 acaaacccaa aaaatcaaaa atccgagtcc aaaacggtcc gaatttgacc aaataatccg
    42661 tctgcggctg gttttgctgt aacaccgtgc cgataaccga tgcaataccc agcagactga
    42721 gcaaagcgac cgcaaagcgc atggagctga aaaaagcgaa ccacggacgg gaaagaagtg
    42781 tgggggatat acgggattta ctcattgtgt gtttattccg ccatcaggaa tatgggaaag
    42841 cagaattggg cagacagaaa acaacgtccc gattctactg tcttgatgct ttttatttca
    42901 agacaatgaa gacagcctgc atcgattcca acggttgcga ttgaaaaaac ttatcgcaaa
    42961 attttcagaa gccgtctgaa aaccttttca gacggcttct gaagcagacc cttggatgat
    43021 taacgcaaac cctggataaa gttagcaact gcttttaaat cttcttcgga catacggttt
    43081 gcaatatctt ccatgatggt atttttacgc tgaccggact tgtaggcatt catctgttca
    43141 acaatatatg cctggtgctg gccgcccaaa cgcggataag cctgaatttc gcttccgcct
    43201 cccggcatac ccgcaccgct cggaccgtgg caggacatac acgccggcac ttttttatcg
    43261 ctcaaaccgc cgcgatagat tttcgcgccc aattcgggat tttccttagg attggcttcg
    43321 ccggatttgg gctgctgttt ggcatagaat gcggatacgt tcaaaatatc ctgatcgctc
    43381 aaattcatta ccaccggttt catcacagct gccgaaccgt gggtgcgttt gccgtcgcgg
    43441 atgccgatag tttgatgata gatgtaagca gtatgctgtg ccgccaaacg cggatacatc
    43501 gcaatgccgc tgttaccgtc tgctgcatgg caagccgcac aaaccgttgc ggcaacctgt
    43561 ttgccttttt ccacgtctgc tttgggagat gcggaaaccg caccggcagc caaaacaaag
    43621 gccaataaag tcaatcgtct catggagtgc tcctgattac agcattggat aacgcaacaa
    43681 tgctcttttt tatattcaaa tatgggattt ttgacccgat taaaaccgat gattctgtaa
    43741 acgtgttatt ctatactaaa tttacattaa attaccactg tgtttcacat aaaaccaacc
    43801 gcatattttt gctgtcggac aaacggcggc ggaaaacaag gatatgccca tgaacctttt
    43861 tcaaaacgcc aaattcttca cgacggtaaa ccatctcaaa gacctgccgg acacaccgct
    43921 cgaaattgcc tttgtcggca ggagcaatgc cggaaaatcc agtgccatca ataccctgac
    43981 caaccatgtc cgtcttgcct acgtttcaaa aacaccggga cggacacagc atatcaactt
    44041 cttcgagctg cagaacggca attttatggt cgatttgccc ggctacggtt atgcccaagt
    44101 ccccgaagcg gtacgcgcac attgggtcaa tctgctcggc gactacctcc gccatcggaa
    44161 acagcttatc gggctggttt tgattatgga tgcccgccat cctttaaaag aactcgacat
    44221 ccgtatgctg gactttttcc acacgaccgg cagaccggtt cacatcctgc tgtcaaaagc
    44281 cgacaaatta tccaaaaacg aacagataaa aaccctgtcc caagtcaaaa aactgctcaa
    44341 accttattcc gacaggcaaa acatcagcgt acagctgttt tccagcctga aaaaacaagg
    44401 tattgacgag gccaaccgaa ctgtcggaag ctggtttgac gcagcagatg ccgccgcttc
    44461 ctctccagag gaaaactgac ctcaattata cggaaaccgt atccccccac ttgaccggcc
    44521 gcaaacattt aaaaaattgc cactgccaaa tctaaaatgc cgtctgaaag acttttcaga
    44581 cggcattttg cggagtcttt aaaacaggga atccaactgc tgctgtttgg aatccgtatt
    44641 gctcggaagc accggcgttt cctgtacgtc ctgacgcgtt tcgtcagacc ttccctgctg
    44701 ttcgttttct actgccgctt catcatcttc ttttgcccgt cgggaaggtt gcggcgcaat
    44761 accgctgttg tccagcatca agcccggatc ggttaccata cgttccttca tatagtattc
    44821 gccattgctg ctgaccacac cttcaggcat tttcatccct ttgccctgct ttcctttcaa
    44881 cgcaaaacgc atatagtcca cccaaaccgg caccgcaatc gtaccgccgt agccggcacg
    44941 ccccatactc ttaggtttgt cgaagccgat atatacggca gtaaccacat cagggttaaa
    45001 accgacaaac cacgcatctt tattgtcgtt ggtcgtaccc gttttaccgg caatatccgt
    45061 tcttcccaac gcagctgccc cccttgccgt accgacacgg accacatcct gcataatctt
    45121 atacataata taggcattgc gcggatcgat tgcctgaggc gcattttgcc ctgccaccaa
    45181 aggttgcatt tgggcgcgca acctgccgtc tctgtcataa atcttatcga tcacgtgcga
    45241 agaaacccta tatccgccgt tcgcaaatac actatatgcc tccgccactt tcaacggcgt
    45301 cgtctcgccc gtacctaaag ccatagacag gcttgccggc agctcggacg gcctgaagcc
    45361 gaaacgccgg atatactgtt gcgcgtaacc gacaccgata gacatcaaaa tacggatgga
    45421 aaccatattc ttggaagccg tcagagcctg tctcaaagta atgtagccgg aatatctgcc
    45481 gtctgaattt ttaggtgtcc aaaccgaacc gttcggccct ttccccggca gggaaatcgg
    45541 cgcatcgtta accactgtgg acgcggtcat ccccttagat aatgccgccg aatagacaaa
    45601 cggcttaaag gtcgaacccg gctgccgcat tgcctgaacg gcacgattga atgttttgct
    45661 gtgaaaatca taaccgccga ccagcgcgcg cacagctccg gtttttgcat ccagcgaaac
    45721 caaagccccc tgcagcaacg gctcttgaac caccgcccaa cgcccgccgt tgtttttgac
    45781 acggatgacc gcgcccctgc ggatacggtc ctcccccatt ttctcattat cgaccgctcg
    45841 ggccgcaaaa cccaaggcgc gcctgtcaag cgcaacccgc ctgccgccgg gcagctgtat
    45901 gacgacattt ttctttttag taacatccaa cacaacggcg ggaaccattt tatcgacggt
    45961 atagagtccc gacagatact ggctgacagt ctcctcgaca tcttcactct tactcaaatc
    46021 gatatagttt tccgcaccgc ggtagctgct gccgcgatcg aaattccgta gagccttgcg
    46081 caatgcctcg gttgccgcct tctgatgatc ggtgcggacc gtggtataaa ccttaaaacc
    46141 ctgcgtatag gcatcttcac catatttctc atacagttcc cgacgcacca tttccgccac
    46201 atataaagca ctctgatcga ttttccgaac aaaccgctca taatgcagtt cctcattcaa
    46261 tgcctgatcg cgctgttgca cggtaatcat cttctcctcg agcatattgt tcaaaatata
    46321 cttctggcgc aacttggcac gctccggatt aacaatcgga ttataggcag acggagcctt
    46381 gggcagtccc gcaagcatgg cggcttccgc caaagtcaaa tctcggacat tcttattgaa
    46441 atagatttgc gcggcagatg caaaaccata ggcgcgctga ccgaggtaaa tctgattgaa
    46501 atacaactca aggattttgt ctttgcttaa agactgctcg attttatagg caagcaacac
    46561 ctcattgaat ttgcgtgtga acgttttttc actgctcaaa taaaaatttt tcgccacctg
    46621 ctgtgtaatc gtactcgcac ccgactgcac gctgccggac acgacattgc cgacggcagc
    46681 gcgggcaaca ccccaaacat ccacccccca atgccggtaa aagcgtttat cctcggcggc
    46741 aataaccgca ttccgcaaca cctcggggaa atcgccgatt tttgtaaatt cgcgccgctg
    46801 ctccccatac ataccgatga cttctccatc cgccgaataa atagtcaacg gcattttagg
    46861 ctggtaatgc tgcaaagaat ccaaagacgg cagtttcgga tacgttacca aaatagcaat
    46921 ggcaaccaga cccactccaa atacacaaaa accaaaaaac aaaccaaaac aagtagttaa
    46981 aatcttttta atcatagctg aataataatt taccattatt ggtattaaat aaagtaaaat
    47041 agcaaccgat ttctacaaag cacggtttca atgtgcaaag aacaaggaat ccattacgga
    47101 taccgaaacg gttactcact gtacaaataa agcaggaact ttcatcatgc gcttgtttaa
    47161 aagcttgaaa aaccctaaaa aaacagatgc caagctccct aaaaaatctt cgggactcaa
    47221 taaccgcgcg gcaatcggca tcgatatcga ccagcattcc atcaaaatgg tacaattgtc
    47281 aggacgtagt ttaaaccaaa ttcaattgga aaaatacgtc attgccaaat taccaaagaa
    47341 tatcattcaa ggcaataaag tccaaaatta cgatcaactt gttacatatt tgcaacaagc
    47401 ctatgccaaa ctgggtactt cgtgcaaaaa catcgtcgcg tccgtcccgc aaaatctggc
    47461 aaccatcgaa caattgacct acacggccaa agatgcagaa ttggacctgc aggggttcgt
    47521 ggagtcctcc atctccgaag ccagctcgat atcgctcgaa gaagccaatt acgactatca
    47581 ggtcttgtcc caatcggccg tcggcgaagc tgttttgtcc gtcgcatcga gaaaggatga
    47641 aatcgaaccc ctgattgacg cattcaacgc agccggtatg aaattatccg cgcttgatgt
    47701 ggacattttc ggacaataca acgcctacgc gctgtggata aaccattttg cccccgagct
    47761 tgcggacgaa aaagtggcca ttttcggcgt atatgccgca cagacctacg ccctggtcat
    47821 ccaagacgga aaaatcctat acaaacagga aacctccgtc agcgaagaac agctcaacca
    47881 actcatccag cgcacctatc aggtaacagc agaaaaagcg gaagagatca tcaactcccc
    47941 gcaaaaacct tccgattacc aagaaagcgt ggcaaactat ttcaaccagc agattaccca
    48001 agaaatacaa agggtcttgc agttttatta caccacgcag accgcagacg atatgaccga
    48061 catcaagcat atcctgctga cgggggaagc ggtgcgccag aaaggcatcg cccaaaccgt
    48121 cgcctcgcaa accaatgcgg atgtacaatg cgtccatccc gcgcgttatt ttgcgaacga
    48181 cctcaaaaca gacgaacaac aattcgaact tgatgcgccg acactgacca aggcgttcgg
    48241 tttggcggta cggggattat aattatgaac aatttaatca aaatcaacct cctcccctac
    48301 agggaagaga tgaacaaacg caaacagcag cagtttaaaa cgctgatgta cggcgccgtg
    48361 ctgacgggcg ttgccgccgt tgcggcaacc tacctgttta tcgacaatat gatcaataaa
    48421 cagtcggaaa aaaacacgct gctggaaatc tccatcgcac acttggatac cgagctgtcg
    48481 gaaatacaaa agctcaaaca ggaaaaagat gccttcctga ttaagaaaaa caaaatcgag
    48541 gagctccaga tcaaacgcct ccaagccgca aaaatcctcg acagcctgaa tgaggccgtc
    48601 cccggaagca cctacctaac ctcgctggat gccgttaccg ccgactctta tcggctcagc
    48661 ggcaggacat ccagcgacaa ccgcgttgcc gccatgatga gggcgatgcc caataccggc
    48721 atattcaagc aacccgaatt gttaagcatc aagaaaaaca attcgcatca agaatttacc
    48781 cttcaggcaa cattacaacc tatcgtaaag gcggccgaat ccaaagagaa tccggcttcg
    48841 ggaaacgcac aggaggcaaa ctgaatggct tctaaatcat ctaaaaccaa cttggatctc
    48901 aacaaccttc acctgctcaa ccttcctgcc aggcttttta tcgccctgct ggtcgttgcc
    48961 gccgtgctgg ggctcggtta tgccggattg ttcaaaagcc agatggaatc ccttgaggaa
    49021 tatgaagcaa aagaaaccga actgaaaaac acctacaaac agaaaagtat cgacgcggcc
    49081 agcctgaaca accttaggga cgaacttgcc tcaatccgct ctgccttcga tatcatgttg
    49141 aaacagctgc cgacagatgc agaaattccc aatttggttc aagagcttca tcaggcgggt
    49201 tcgagcaacg gtctgcgctt ggacagcgtt atgccccaac ctcccgtaga tgacggtccc
    49261 atcaaaaaat taccctattc catttccatt accggaaatt acgaacagat cagccaattt
    49321 acccgcgatg tcggcagtct ctcccgaatc attacccttg agtcgctgaa aatcgcccaa
    49381 tctccggaaa acggcggcaa tcctgacggc aagagcagta tcctgaacct cagcgccatt
    49441 gccaccacct accaagcaaa atccgtagaa gagcttgccg cagaagcggc acaaaatgcc
    49501 gagcaaaaat aacttacgtt agggaaacca tgaaacacta tgccttactc atcagctttc
    49561 tggctctctc cgcgtgttcc caaaattctg aagacctaaa cgaatggatg gcacaaacgc
    49621 gacgcgaagc caaagcagaa atcatacctt tccaagcacc taccctgccg gttgcgccgg
    49681 tatacagccc gccgcagctt acagggccga acgcattcga cttccgccgc atggaaaccg
    49741 ccaaaaaagg ggaaaatgcc cccgacacca agcgtattaa agaaacgctg gaaaaattca
    49801 gtttggaaaa tatgcgttat gtcggcattt tgaagtccgg acagaaagtc tccggcttca
    49861 tcgaggctga aggttatgtc tacactgtcg gtgtcggcaa ctatttggga caaaactacg
    49921 gtagaatcga aagcattacc gacgacagca tcatcctgaa cgagctgata gaagacagca
    49981 cgggcaactg ggtttcccgt aaagcagaac tgctgttgaa ttcttccgac aaaaacaccg
    50041 aacaagcggc agcacctgcc gcagaacaaa attaagaaga ggattactcc attatgaata
    50101 ccaaactgac aaaaatcatt tccggtctct ttgtcgcaac cgccgccttt cagacggcat
    50161 cggcaggaaa cattacagac atcaaagttt cctccctgcc caacaaacag aaaatcgtca
    50221 aagtcagctt tgacaaagag attgtcaacc cgaccggctt cgtaacctcc tcaccggccc
    50281 gcatcgcctt ggactttgaa caaaccggca tttccatgga tcaacaggta ctcgaatatg
    50341 ccgatcctct gttgagcaaa atcagtgccg cacaaaacag cagccgtgcg cgtctggttc
    50401 tgaatttgaa caaaccgggc caatacaata ccgaagtacg cgggaacaaa gtttggatat
    50461 tcattaacga atcggacgat accgtgtccg cccccgcccg cccagccgta aaagccgcgc
    50521 ctgccgcacc ggcaaaacaa caggctgccg caccgtttac cgagtccgta gtatccgtat
    50581 ccgcaccgtt cagcccggca aaacaacagg cagcggcatc ggcaaaacaa caggcggcga
    50641 caccggcaaa acaaaccaat atcgatttcc gcaaagacgg caaaaatgcc ggcattatcg
    50701 aattggcggc attgggcttt gccgggcagc ccgacatcag ccaacagcac gaccacatca
    50761 tcgttacgct gaaaaaccat accctgccga ccgcgctcca acgcagtttg gatgtggcag
    50821 acttcaaaac accggttcaa aaggttacgc tgaaacgcct caataacgac acccagctga
    50881 ttatcacaac aaccggcaac tgggaactcg tcaacaaatc cgccgcgccc ggatacttta
    50941 ccttccaagt cctgccgaaa aaacaaaacc tcgagtcagg cggcgtgaac aatgcgccca
    51001 aaaccttcac aggccggaaa atctcccttg acttccaaga tgtcgaaatc cgcaccatcc
    51061 tgcagatttt ggcaaaagaa tccgggatga acattgttgc cagcgactcc gtcagcggca
    51121 aaatgaccct ctccctcaaa gacgtacctt gggatcaggc tttggatttg gttatgcagg
    51181 cgcgcaacct cgatatgcgc cagcaaggga acatcgtcaa catcgcgccc cgcgacgagc
    51241 tgcttgccaa agacaaagcc ttcttacaag cggaaaaaga cattgccgat ctgggcgcgc
    51301 tgtattccca aaacttccaa ttgaaataca aaaatgtgga agaattccgc agcatcctgc
    51361 gtttggacaa tgccgacacg accggaaacc gcaacacgct tgtcagcggc aggggcagcg
    51421 tgctgatcga tcccgccacc aacaccctga ttgttaccga tacccgcagc gtcatcgaaa
    51481 aattccgcaa actgattgac gaattggacg tacccgcgca acaagtgatg attgaggcgc
    51541 gtatcgtcga agcggcagac ggcttctcgc gcgatttggg cgttaagttc ggcgcgacag
    51601 gcaggaaaaa actgaaaaat gagacgagcg cattcggctg gggcgtgaac tccggcttcg
    51661 ggggcggcga taaatgggag gcccaaacca aaatcaacct gccggttgcc gctgccgcaa
    51721 acagcatttc gctggtgcgc gcgatttcct ccggcgcgtt gaatttggaa ttgtccgcat
    51781 ccgagtcgct ttcaaaaacc aaaacgcttg ccaatccgcg cgtgctgacc caaaaccgca
    51841 aagaggccaa aatcgaatcc ggttacgaaa ttccttttac cgtaactaca gcctcgggcg
    51901 gcagcaactc taccaacacg gaactcaaaa aagccgtctt ggggctgacc gttacgccga
    51961 acatcacgcc cgacggacaa atcatcatga ccgtcaaaat caacaaagac tcgcctgcac
    52021 aatgtgcttc aggcaacaac acaatcctat gtatttcgac caaaagcctg aatacgcagg
    52081 ctatggttga aaacggcggc actttgattg tcggcggtat ttatgaagaa aacaacggca
    52141 atacgctgac caaagtcccc ctgttgggcg acatccccgt tatcggcaac ctctttaaaa
    52201 cacgcgggaa aaaaaccgac cgccgcgaac tgctgatttt cattaccccg aggattatag
    52261 atacggccgg caacagcctg cgctattgat gcgtcaaaat aagggcatat gttttacagc
    52321 atatgccctt tctttatgct ttttgccgcg accgaaatgc cgtcattccc gcgcaggcgg
    52381 gaatccaact tgtccggttt cggttgtttt tcgtctcgta acttttgagc cgtcattccc
    52441 gcgaaatcgg aaatccagtc cgttcagttt cggtcatttc cgataaattc ctgttgcttt
    52501 tcatttctag attcccactt ttgtgggaat gacggcggaa gggataaatc ctcgcaatcc
    52561 aaagcctcgt catttccaca aaaaacagca acccgaaaca ccccgtcatt cccgagcagg
    52621 cgggaatcta ggtctttaaa cttcggtttt tttccgataa attcttgcct cattgaaatt
    52681 ctagattccc gctttcgcgg gaatgacggg atatgggttt ccgtgcggac gtgttcggat
    52741 ttccgcctgc gcgggaatga cgattcgggt attactgacg attcatcggg ccgtagggtg
    52801 ggcttcagcc cactaatttc accgcatcaa gggttttggg gaataggcgt aactcggcgg
    52861 tttcggtttt tggcgaagtt ggttggaatc ggcgggctga agcacaccct acagtccacc
    52921 ctacaaccca tcttacgcat ccgccttacg catttatcct tcgcctgccg atatgccgtc
    52981 attcccgcac aggcgggaat ctagaaccgc aacgcggcag gaatctgtcg gatacggctg
    53041 aaaccgaacg gactggattc ccgcctgtgc gggaatgacg gctgcagatg cccgacggtc
    53101 tttatagcgg attaacaaaa atcaggacaa ggcggcgaag ccgcagacag tacggatagt
    53161 acggaaccga ttcgcttggt gcttcatcac cttagggaat cgttcccttt gagctaaggc
    53221 ggggcaacgc cgtaccggtt tttgttcatc cgctatagta ttgataaaca tattatcttc
    53281 aatatattca attggataat tgtttaccta agcaaagata attgcctttt cctgacaaat
    53341 taagtgaaat caacggattg tcaaaacaca gcctgaaata aaaaacctcc ctgatttctt
    53401 ttatttttcc ttaaaatcag aaaagttcgg gatggtcggg ttatttttcc aaacgtaccg
    53461 ccgccctgcc gatttcgtat aaaatcccgc cgtaacccga caagcccgaa ccctgccgcc
    53521 ccgaaaggcg ggggggtcaa acattaagga accgtgatga aaaactttaa cggcaaactc
    53581 atcctcatcg gactgatggg cgcgggcaaa actacgctgg gccggcaaat ggcgcagcgg
    53641 ctggattacc gtttttacga cagcgatcac gaaatcgccg cagcggcggg cgttcccatc
    53701 cccaccatat ttgaaatgga aggcgaacag ggattccgtt cgcgcgaaac cgccatactc
    53761 aaaaaactga ttgtcctgcc gcatatcgtc ctgtccaccg gcggcggcgc ggtgttaaaa
    53821 gaagaaaacc gcgcccttat ccgcaaaagc ggcacggtcg tttatctgca cgccccgccc
    53881 gaaaccctgc tcgaacgcac gcgctgcgac aacagccgcc ctttgctgca agttgccgat
    53941 cctttggcga aattgcgcga actctacgcc gcacgcgacc ccgtttaccg ccaaaccgcc
    54001 gactttaccg tagaatcggc aaactgccgg gaaaccgtgc aaaccctgct caaacgctta
    54061 tcccgataaa ccggcatatg cgccgctccc cgaaaaccaa accgcacccc gcccggcggg
    54121 ccggcggttc aaactttaag gaacaacaat gaaaacactg accgtacaca cgccttcgca
    54181 cagctacccc atctttgtcg gcaacgggct gctgccgcag gcgggaagcc tgctcaaacc
    54241 gcatttgggc aaacgcgccg ccatcatcac caacgaaacc gtcgccccgc tctacctcgg
    54301 cacgcttcag acggcattgg atgcggcagg cgtatcccat ttcagcatca tcctgcccga
    54361 cggcgaggcg cacaaaaact ggcagacgct caacctcatc tttgacgggc tgatgcaaaa
    54421 ccgcgccgaa cgtaaaacca cgctgattgc gctgggcggc ggcgtgatcg gcgacatggt
    54481 cggctttgcc gccgccacct accagcgcgg cgcgccgttc atccaaatac cgaccacgct
    54541 gttgagtcag gtcgactcat cggtgggcgg aaaaaccgcc atcaaccacc cgctcggcaa
    54601 aaatatgatc ggcgcgtttt accagccgca ggcggtgctt gcagatttgg acacgctgca
    54661 caccctgccc gcccgcgaac tgtccgccgg tatggcggaa gtcatcaaat acggcgcgct
    54721 cggcgacatc ggcttttttg aatggctgga acagcatatg cccgaactga tgacactcga
    54781 acgcgcgccc ctcacccaag ccgtgtaccg ctgctgccaa atgaaggcag acatcgtcgc
    54841 ccaagacgaa accgaacagg gcatacgcgc atggctcaac ctcggacaca ccttcggaca
    54901 cgccgtcgaa gcggaaatgg gttacggcgt atggctgcac ggcgaggccg ttgccgccgg
    54961 ctgcgtgttg gcggcgcgtt tgtccgaaca actgggcaaa acctccgccg cagataccgc
    55021 gcggctcgcc gccctgctcg aagccgccgg actgccgtcc gcaccgcccg tgtttgcctt
    55081 tgaaaaatgg ctggcacata tgagccacga caaaaaagtc agcggcggca tcatgcgctt
    55141 tatcggtctg aaccggctgg gcgaagccgt catcaccgaa attaccgaca cggacatcct
    55201 ccggcgcacc ctgcaaccgt atctctgatt tcctctgccg atgtgttgcc gcgcgggttt
    55261 gacgcacgat gatgactttc catcatcatt ctccgcgaaa gcgggcatcc ggttattcca
    55321 tattgcaggg atttatccgg aataacgaca acctgtccgc cgtcatttcc gcgcagatgg
    55381 aaatttggaa attcaaaatc acaggaattt atccggaata tagcggatta acaaaaatca
    55441 ggacaaggcg gcgaagccgc agacagtaca aatggtacgg aaccggttcg cccggtgctt
    55501 gggcgcctta gggaatcgtt ccctttgagc cggggcgggg caacgccgta ccggtttttg
    55561 ttaatccgca acagcaaccc tgtcgccgtc attaccgcga aagcgggaat ccagtccgtt
    55621 cagtttcggt catttccgat aaattcctgt tgcttttcat ttctagattc ccactttcgt
    55681 gggaatgacg gcggaagggt tttggttttt tccgataaat tcttgaggca ttgaaattcc
    55741 agattcccgc ctgcgcggga atgacggctc aaaagttacg gaacgaaaaa caaccgaaac
    55801 cggacaagtc ggattcccgc ctgcgcggga atgacggaat cttaagtttc cgtctttgtt
    55861 ttctgttttc gcgggaataa tggggaacaa gccgtatttc agacggcatt ttcagttcgg
    55921 ggtataatcc gaatacttgc gaccatctga atcatttgga caaactatgt gtcaactgct
    55981 gggcatgaac tgcaatacgc cgaccgatat tatgttttcc tttgaaggct tccgccgcag
    56041 gggcggcatt accgaccatc acgccgacgg tttcggcatc ggctttttcg aaggcaaagg
    56101 cgtgcgcctg ttccatgacg acaagccgag tgcaaattcc cccgtcgccg acctcgtgcg
    56161 cgcctaccag attaaatcgg aaaacgtcgt cgcccacatc cgcaaagcct cgcaaggtca
    56221 aacctcgctg gcgaacaccc atccctttat gcgcgaaatg tggggcggct actggctgtt
    56281 tgcccacaac gggcatttgg tcgatttttt ccccgaacag ggagaatttt tccaccccgt
    56341 cggcacaacc gattccgaac gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt
    56401 tgccgcccgt cccgacgacg acacgctgtt tgacgcgatt gcggggctga cgcacgaaat
    56461 ccgcaagttc gggctgttta acttcatgct ttcagacggc atttccctgt ttgcccacgc
    56521 cagcacgctg ctgcactaca tcgtccgcca agccccgttc ggcaaagcgc gcctgctcga
    56581 cgacgatgta atggtcgatt ttgccgaagt aaccacgccg gacgaccgcg tcgccgttat
    56641 ctccaccctg ccgctgactc gcgatgaatc gtggtcccaa cttgccgtga acgaactggt
    56701 catgttccgc gaaggcaaca tcgtccggca cgaccgtccc gaaaatcccg tctatatgag
    56761 tgccgaagaa ggcctggaaa tcgcccgcgc cgccggcgta tcggtttgac gttcagacgg
    56821 catcaggaag aaacaccatg aaatgcccgt tttgcagcca cccagacacc cgagttgccg
    56881 attcccgcct gatggaagag cgcaataccg tccgccgccg ccgccactgc cccggctgcg
    56941 gcaaacgctt cggcacgctc gaaaccgtcg aactcaaaat gcccgccgtc atcggtccgg
    57001 acaagaaacg ttcgcccttc aatgcacaac gcctccgcaa cgacctgacc gccgccgccc
    57061 gaaaatccgc cctgacaccc gaacagattg acgaaaccgt ccgcctgacg gaacacaggc
    57121 tctacacttc gggtcagcgc gacatatcct catccgaact tgtcgaaatc gtgctggagg
    57181 agctgttcgg gcaaaacacg gaagccgccg tccgtttcgc cgccctgcac aaacgcttcg
    57241 acagcccgga acatttcgcc tcgtggctgg cgcaagccgt caaaacaggc ggcaaaacct
    57301 gattccccca acccatactg atacggtatc cctatgtttt cggacacaaa tatatccatg
    57361 atggaaaacg ccctccgact tgccgctttg gggcgttttt ccacttcacc caacccgcgc
    57421 gtcggctgcg ttatcgcaca cggcagacaa attgtcggac agggcttcca cgtcaaagcg
    57481 ggcgaacccc atgccgaagt ccacgccctg cgtcaggcgg gggaaatggc aaaaggcgcg
    57541 accgcctttg ttaccctcga accgtgcagc cattacggac gcacaccgcc ctgcgccgaa
    57601 gccctgctcc gttccggcgt aacacgcgtc gtcgccgcca tgcgcgaccc caacccgccg
    57661 gttgcaggca aagggcttgt cctgctcaaa gcagcgggca tcaagacgga atgcggacta
    57721 cttgaaaaca aagcacgcga actcaaccgg ggatttttat cgcgcatcga acgccgccgc
    57781 ccctttgtcc gcctcaaatg cgccgtttcg ctggacggca aaaccgccct ttcagacggc
    57841 agcagctttt ggattaccgg cgaggaagcg cgtgccgacg tacaggtttt gcgtgccgaa
    57901 agctgcgcgg tgctgaccgg catcggcacg gtgttggcgg acaatccccg gctcaacgtc
    57961 cgcgcttttc caactttgcg ccaacccgca cgcatcgttt tagacagccg cctgcgcctg
    58021 cccccgaaca gccatttggt taccgacgga caatctccga cctacatcgc caccttggaa
    58081 cgcgatgaag acaaactccg cccctatcgg gaacacgcac acatccgcat cctgatgccg
    58141 tctgaaacgg cagacggcaa aatcgacctg caccatctga tgcgcctcct tgccgacgaa
    58201 ggtttcggcg aaatcatggt cgaagcaggc tccgaactca catccgcatt tttggcggaa
    58261 aatttggcgg acgaaatcgt tttataccgt tcgcccaaaa tcctcggcat cggcagcggt
    58321 ttattttgcc tgcccgaaaa ccgcgccgcc ctttccgcgc cgccattatg gacacccgtt
    58381 tccaccgaaa tcctcggaca cgacatcaaa accgtgttcc ggaaaaacgg caacgccttt
    58441 taaagggttt gccgccgttt tgctatataa taacgccgat aaaaaacggc tcgttcagac
    58501 cgccggcagc cgcgacggcc tgaaagccgt caaattccga tcaagaaagg cttcagacgg
    58561 cacaggcagc atcccgccgc cgaccggaca tcaaaaatgg acacaaaaga aatcctcggc
    58621 tacgcggcag gatcgattgg ctgcgcgatt ttagccgtca tcatcctgcc gctgctgtcg
    58681 tggtatttcc ccgccgacga catcgggcgc atcgtgttga tgcagacggc ggcgggactg
    58741 gcgatgtcgg tgttgtgcct cgggctggat caggcatata tccgcgaata caatgccgcc
    58801 gccgacaaag acattttgtt caaagccctg ttcctgccgc cgctgctgtc tgccgccgcg
    58861 atagccgcct tcctgctttc ccacccggcc ctgccgtctg aaatcctgtt ttcgctcgac
    58921 gatgccgccg ccggcatcgg gctggtgctg tttggactga gcttgctgcc catccgcttt
    58981 ctcttactgg ttttgcgtat ggaagggcgc gcccttgcct tttcgtccgc gcaactcgta
    59041 cccaagtccg ccctgctgct gttgctgctg acggtctggc tgctgcactt tccggcgcgc
    59101 accgccgtcc tgacctccgt ttacgcgctg gcaaaccttg ccgccgccgc ctttttgctg
    59161 tttcaaaacc gatgccgtct gaaggccgta tggcgcgcac cgttttcgcc cgccgtcctg
    59221 catcgcgggc tgcgctacgg cataccgctc gcactggaca gccttgccta ttggggactg
    59281 acatccgccg accgtttgtt cctgaaaaaa tatgccggcc tggaacagct cggcatttat
    59341 tcgatgggta tttcgttcgg cggggcggca ttattgttcc aaagcatctt ttcaacggtt
    59401 tggacaccgt at
//
LOCUS       DAXFZR010000002        47250 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000002 DAXFZR010000000
VERSION     DAXFZR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 47250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47250
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.002"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            236..2497
                     /gene="nuoG"
                     /locus_tag="ACFJAS_000058"
     CDS             236..2497
                     /gene="nuoG"
                     /locus_tag="ACFJAS_000058"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC3981433.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASDRLVRVGGVGIYHTDSIVRRSALLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
     gene            2500..3576
                     /gene="nuoH"
                     /locus_tag="ACFJAS_000059"
     CDS             2500..3576
                     /gene="nuoH"
                     /locus_tag="ACFJAS_000059"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC3981434.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPRGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            3657..4136
                     /gene="nuoI"
                     /locus_tag="ACFJAS_000060"
     CDS             3657..4136
                     /gene="nuoI"
                     /locus_tag="ACFJAS_000060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC3981435.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            4148..4819
                     /locus_tag="ACFJAS_000061"
     CDS             4148..4819
                     /locus_tag="ACFJAS_000061"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC3981436.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            4816..5121
                     /gene="nuoK"
                     /locus_tag="ACFJAS_000062"
     CDS             4816..5121
                     /gene="nuoK"
                     /locus_tag="ACFJAS_000062"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC3981437.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            5124..7148
                     /gene="nuoL"
                     /locus_tag="ACFJAS_000063"
     CDS             5124..7148
                     /gene="nuoL"
                     /locus_tag="ACFJAS_000063"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC3981438.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRTKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            7291..7794
                     /locus_tag="ACFJAS_000064"
     CDS             7291..7794
                     /locus_tag="ACFJAS_000064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981439.1"
                     /translation="MDKRLFDGTVRFEHVTGVGTMQVDFQPNQRVYTMIGENGVGKTK
                     FLECLFTVLLFTYRMANINIGFSWIDLQRLPLKIVELPNGKNEDFRRFSERSNQGFNI
                     NEDVLFSGYQHRLPFVYLPAQSRGWVKKQTSKLSRWEVLKGVQANIWGICFLFFNLAI
                     VHSRLNP"
     gene            7797..8459
                     /locus_tag="ACFJAS_000065"
     CDS             7797..8459
                     /locus_tag="ACFJAS_000065"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC3981440.1"
                     /translation="MENDLEEWIIQRARSANPYQANEDNRKIEIDTLLFLLNQFDGRI
                     APDFLEIKGDDRIFIKLDNQKRELSGLSSGFTSILKIMQSIVAGYSYFTNEQQIANVR
                     GVVLIDEIESHLHNEWQIKIVPLLKKLFPNTTFFITTHSSLVISQLEQGGAYRLVRSK
                     EDGVVYGKMIDYPSNASFVDLLNEAFGVDLNKKKIERAQERNQEQAKKPYWNWYVRNW
                     AD"
     gene            8470..8694
                     /locus_tag="ACFJAS_000066"
     CDS             8470..8694
                     /locus_tag="ACFJAS_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC3981441.1"
                     /translation="MTVYLLDTNYLVYLADDDSDEEKRKAVLSDMAEKLQQDDNRFVI
                     TPLIRYEVLRGVDWGKSEKLSRLTGVLAQF"
     gene            8725..8931
                     /locus_tag="ACFJAS_000067"
     CDS             8725..8931
                     /locus_tag="ACFJAS_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981442.1"
                     /translation="MASNLYRFDKFEAERDNTPKNLEKRKFDMFHYATASVNNLEILS
                     HDTDVNKIKDLHERMRLEDSAELA"
     gene            9041..10537
                     /locus_tag="ACFJAS_000068"
     CDS             9041..10537
                     /locus_tag="ACFJAS_000068"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC3981443.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            10547..11992
                     /gene="nuoN"
                     /locus_tag="ACFJAS_000069"
     CDS             10547..11992
                     /gene="nuoN"
                     /locus_tag="ACFJAS_000069"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC3981444.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            12104..12403
                     /locus_tag="ACFJAS_000070"
     CDS             12104..12403
                     /locus_tag="ACFJAS_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC3981445.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(12520..13416)
                     /locus_tag="ACFJAS_000071"
     CDS             complement(12520..13416)
                     /locus_tag="ACFJAS_000071"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC3981446.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(13400..13624)
                     /locus_tag="ACFJAS_000072"
     CDS             complement(13400..13624)
                     /locus_tag="ACFJAS_000072"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC3981447.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            complement(13737..14660)
                     /gene="rsgA"
                     /locus_tag="ACFJAS_000073"
     CDS             complement(13737..14660)
                     /gene="rsgA"
                     /locus_tag="ACFJAS_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC3981448.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            complement(14665..16521)
                     /locus_tag="ACFJAS_000074"
     CDS             complement(14665..16521)
                     /locus_tag="ACFJAS_000074"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3981449.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSAAALGIWLWHNGQVGVGAVATATAMALRANGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRSGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            complement(16547..16684)
                     /locus_tag="ACFJAS_000075"
     CDS             complement(16547..16684)
                     /locus_tag="ACFJAS_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981450.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            complement(16684..17268)
                     /gene="ruvA"
                     /locus_tag="ACFJAS_000076"
     CDS             complement(16684..17268)
                     /gene="ruvA"
                     /locus_tag="ACFJAS_000076"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC3981451.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGVKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDRLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(17350..17676)
                     /locus_tag="ACFJAS_000077"
     CDS             complement(17350..17676)
                     /locus_tag="ACFJAS_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC3981452.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(17737..18423)
                     /locus_tag="ACFJAS_000078"
     CDS             complement(17737..18423)
                     /locus_tag="ACFJAS_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC3981453.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            complement(18551..19015)
                     /gene="trmL"
                     /locus_tag="ACFJAS_000079"
     CDS             complement(18551..19015)
                     /gene="trmL"
                     /locus_tag="ACFJAS_000079"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC3981454.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            complement(19080..19805)
                     /locus_tag="ACFJAS_000080"
     CDS             complement(19080..19805)
                     /locus_tag="ACFJAS_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC3981455.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCAADLAAYR
                     TDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRALKHLADLGM
                     AQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            19804..20553
                     /gene="bioH"
                     /locus_tag="ACFJAS_000081"
     CDS             19804..20553
                     /gene="bioH"
                     /locus_tag="ACFJAS_000081"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC3981456.1"
                     /translation="MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADGIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLAR
                     CGTPQALQEALDAAERADVRHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEVFAALYRDFVEGGLT"
     gene            20550..21338
                     /locus_tag="ACFJAS_000082"
     CDS             20550..21338
                     /locus_tag="ACFJAS_000082"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3981457.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPLAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCAGAIFEREGGLGITLETVYGHAVKKLVLPQGENVVRFFPKR"
     gene            complement(21419..21526)
                     /locus_tag="ACFJAS_000083"
                     /pseudo
     CDS             complement(21419..21526)
                     /locus_tag="ACFJAS_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            21648..23021
                     /locus_tag="ACFJAS_000084"
     CDS             21648..23021
                     /locus_tag="ACFJAS_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC3981458.1"
                     /translation="MDWRGNKPLGAAELADLKPLYKDFMYWERGLHMYKASAVVPTGY
                     VRVGNTAPLCGEDTQRYASFWGDGYDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAG
                     REYGISKQNLSDVWDDFEDAMELKAFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLEN
                     HGQTVLDFSNTRITDLSVDMTGVESLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLIL
                     EVGKSVPKVRGLENLTAVNVMGIADFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSG
                     FEDLEVFTAVDLFGFGADDIPHPDRLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLD
                     LWIEKARKPEWLAQNFDNPFRDWDGAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPEN
                     TGEGLAEAVKAYTGGFNKMDKKHFIDTVEREDIAEALETILDLIPDGSCADKEKLFEI
                     FDKNRNF"
     gene            complement(23058..25361)
                     /gene="recQ"
                     /locus_tag="ACFJAS_000085"
     CDS             complement(23058..25361)
                     /gene="recQ"
                     /locus_tag="ACFJAS_000085"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC3981459.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSGEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(25424..26206)
                     /gene="trpC"
                     /locus_tag="ACFJAS_000086"
     CDS             complement(25424..26206)
                     /gene="trpC"
                     /locus_tag="ACFJAS_000086"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC3981460.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            complement(26257..27213)
                     /locus_tag="ACFJAS_000087"
     CDS             complement(26257..27213)
                     /locus_tag="ACFJAS_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFC3981461.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRRELIPDSTGKT
                     KTAYDFSGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFALDTLAARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEAV
                     TLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            complement(27346..28884)
                     /gene="murJ"
                     /locus_tag="ACFJAS_000088"
     CDS             complement(27346..28884)
                     /gene="murJ"
                     /locus_tag="ACFJAS_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC3981462.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMVTDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            29322..30020
                     /locus_tag="ACFJAS_000089"
     CDS             29322..30020
                     /locus_tag="ACFJAS_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC3981463.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(30077..31081)
                     /gene="amgK"
                     /locus_tag="ACFJAS_000090"
     CDS             complement(30077..31081)
                     /gene="amgK"
                     /locus_tag="ACFJAS_000090"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC3981464.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            31151..33556
                     /locus_tag="ACFJAS_000091"
     CDS             31151..33556
                     /locus_tag="ACFJAS_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC3981465.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQASDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMEGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSHDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            33658..34659
                     /locus_tag="ACFJAS_000092"
     CDS             33658..34659
                     /locus_tag="ACFJAS_000092"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3981466.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSKNAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(35138..37018)
                     /locus_tag="ACFJAS_000093"
     CDS             complement(35138..37018)
                     /locus_tag="ACFJAS_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC3981467.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEGEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGLFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRANGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            37174..37806
                     /locus_tag="ACFJAS_000094"
     CDS             37174..37806
                     /locus_tag="ACFJAS_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC3981468.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQNHT
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            37878..39248
                     /gene="purB"
                     /locus_tag="ACFJAS_000095"
     CDS             37878..39248
                     /gene="purB"
                     /locus_tag="ACFJAS_000095"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC3981469.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFSRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPVSRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTHGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            39328..41196
                     /locus_tag="ACFJAS_000096"
                     /pseudo
     CDS             39328..41196
                     /locus_tag="ACFJAS_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            complement(41332..41946)
                     /locus_tag="ACFJAS_000097"
     CDS             complement(41332..41946)
                     /locus_tag="ACFJAS_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC3981470.1"
                     /translation="MNFTRLLNQVLSTVQKKGSTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALELDDNLVESLERQLGI"
     gene            complement(41982..44132)
                     /gene="dinG"
                     /locus_tag="ACFJAS_000098"
     CDS             complement(41982..44132)
                     /gene="dinG"
                     /locus_tag="ACFJAS_000098"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC3981471.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(44315..45613)
                     /locus_tag="ACFJAS_000099"
     CDS             complement(44315..45613)
                     /locus_tag="ACFJAS_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC3981472.1"
                     /translation="MPSETALPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(45603..46514)
                     /locus_tag="ACFJAS_000100"
     CDS             complement(45603..46514)
                     /locus_tag="ACFJAS_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC3981473.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            46622..47020
                     /locus_tag="ACFJAS_000101"
     CDS             46622..47020
                     /locus_tag="ACFJAS_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC3981474.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            47069..>47250
                     /locus_tag="ACFJAS_000102"
     CDS             47069..>47250
                     /locus_tag="ACFJAS_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC3981475.1"
                     /translation="MSRQSLQQAAESRRSVYSLNKNLPVGKDEIVQIVEHAVLHTPFS
                     FNSQSARVVVLFGEEH"
BASE COUNT        10026 a        12483 c        13727 g        11014 t
ORIGIN      
        1 gtcattcccg cacaggcggg aatccatagt ggaatttaca gaacccgata tttgaaaagc
       61 agttgccgaa attcaaaaaa tggattcccg cctgtgcggg aatgacggca aggtggcagt
      121 aaacgtttta aacagtattg attgtcaatg aaactcaaaa ggccgtctga agcccatttt
      181 tcagacgatc tccataaaaa attatttatc aaatacccgt aactaggaac gaaccatgtt
      241 acaaatcgaa atcgacggca aacaggtatc tgtggagcag ggcgcgacgg taattgaagc
      301 cgcgcacaag ctcggtactt atattccgca tttctgttac cacaaaaagc tctctattgc
      361 cgccaactgc cgtatgtgtc tggtggatgt ggaaaaagcc cccaaaccgc tgcctgcctg
      421 tgccacgccg gtaacggacg gcatgattgt gcgtacgcat tcggcaaaag cccgagaggc
      481 gcaggaaggc gtgatggagt tcctgctcat caaccatccg cttgattgtc cgacctgcga
      541 ccagggcggc gaatgccagt tgcaggattt ggcggtgggc tacggcaaaa ccaccagccg
      601 ttataccgaa gaaaaacgtt ccgtcgtcgg caaggacatg ggtcctttgg tttccgccga
      661 ggaaatgagc cgctgtatcc actgtacccg ctgcgtacgt ttcaccgaag aaatcgccgg
      721 tttgcaggaa attgcgatgg tgaatcgtgg tgaacactcc gaaatcatgc cctttatcgg
      781 caaagcggtg gaaaccgagc tgtcgggcaa cgtcatcgat ttgtgtcccg tcggcgcatt
      841 gaccagcaaa ccgttccgct tcaacgcgcg tacttgggaa ttgaaccgcc gcaaatccgt
      901 ttccgcacac gacgctttgg gcagcaacct gattgtacaa accaaagacc ataccgtccg
      961 ccgcgtgttg ccgttggaaa acgaagccat caacgaatgc tggctgtccg accgcgaccg
     1021 ttttgcctac gaaggcctgt atcacgaaag ccgtctgaaa aatccgaaaa tcaaacaggg
     1081 tggcgagtgg atggacgtgg attggaaaac cgcgttggaa tatgtccgca gcgcgattga
     1141 atgtatcgcc aaagacggca accaaaacca agtcggcgtt tgggctaatc cgatgaatac
     1201 ggttgaagag ctgtatctgg ccaaaaaatt tgccgacggc ttgggtgtta aaaactttgc
     1261 aacccgtttg cgccaacaag acaaacgtct ttcagacggc cttaaaggtg cgcaatggtt
     1321 gggacaaagt attgaatctt tggctgacaa cgatgccgta ttggtagtcg gtgcgaactt
     1381 gcgcaaagaa cagccgctcc tgaccgcccg cctgcgccgc gccgccaaag accgtatggc
     1441 cttgagcgta ttggccggca gtaaagaaga attgtttatg ccgcttctgt ctcaagaagc
     1501 cgcacatccc gacgagtggg caggccgtct gaaaaacctg tctgccgatg cggaacatgc
     1561 cgttaccgcc agcctgaaaa acgctgaaaa agcagcggtg attttgggtg cggaagtgca
     1621 aaaccatcct gattacgccg ccatttatgc cgccgcgcaa gagttggcgg acgcgaccgg
     1681 cgcagtgctg ggcattttgc cgcaagccgc caacagcgtc ggtgcggacg tattgggtgt
     1741 gaactcgggc gagagcgttg ccgaaatggc aaacacgccg aaacaggcag tcttgctgct
     1801 caacgtcgag cctgaaatcg atacggttga cggtgcaaaa gccgtagccg cgttgaaaca
     1861 ggcgaaaagc gtgatggcgt ttacgccgtt tgtcagcgaa acgctgctgg acgtgtgcga
     1921 cgtattgctg ccgattgcgc cgtttaccga aacatcaggc agcttcatca atatggaagg
     1981 ccgtctgcaa tctttccacg gcgtggtaca aggcttcggc gattcacgtc cgttgtggaa
     2041 agtgttgcgc gtattgggca acctgtttga cctgaaaggt tttgaatacc acgataccgc
     2101 cgcgattctg aaagacgcgc tggatgcgga aagcctgccg tccaaactgg acaaccgcag
     2161 cacatgggca ggggagggcg ttcagacggc ctcagaccgc ctcgtccgtg tcggcggcgt
     2221 cggtatttat catactgatt ctatcgtgcg ccgttccgca ctgttgcaag aaaccagcca
     2281 tgccgccgtg cctgccgcgc gtgtaaatcc gaacacattg gcacgcttgg gcctgcaaga
     2341 cggacaaacc gctgtcgcca aacaaaacgg cgcaagcgta tcggttgccg tcaaagccga
     2401 tgccggtttg cctgaaaacg tggtgcatct gccgctgcat accgaaaatg ccgcgctggg
     2461 tgcgttgatg ggcactattg aactggcggg agcttgatta tgcaggaatg gttccaaaac
     2521 ctctttgccg caacgctcgg tctgggcgat ttgggcatca ccgtaggctt ggtggtatcc
     2581 gtcatcgtca aaatcgtgat tatcctgatt ccgctgattc tgaccgttgc ctacctgacg
     2641 tatttcgaac gtaaagtcat cggctttatg cagcttcgcg tcggcccgaa cgtaaccggc
     2701 ccgcggggtc tgattcagcc gtttgccgac gtgttcaaac tcttgtttaa agaggtaacc
     2761 cgtccgaagc tgtcaaacaa agccttattc tacatcggcc cgattatgtc gctcgccccg
     2821 tctttcgcgg cgtgggcggt gattccgttt aacgaagaat gggtgctgac caatatcaat
     2881 atcggccttt tgtacatcct gatgattacc tcgctgtcgg tttacggcgt gattatcgcg
     2941 ggctgggctt ccaactccaa atattcgttc ttgggtgcaa tgcgtgcttc cgcgcaaagc
     3001 atttcctacg agattgccat gagtgccgcg ctggtgtgcg ttgtgatggt atcgggcagc
     3061 atgaacttct ccgacatcgt tgccgcgcag gcaaaaggca tcgcgggcgg ttctgtgttc
     3121 tcgtggaact ggctgccgct gttcccaatc ttcatcgtct atctgatttc cgccgttgcc
     3181 gaaaccaacc gcgcaccgtt tgacgtggca gagggcgagt ctgaaatcgt tgccggacac
     3241 cacgtcgaat actccggctt tgcattcgcg ctgttcttcc ttgccgaata cattttcatg
     3301 attctgattg ccgcgctgac atcgctgatg ttcctcggcg gctggttgtc tccgttcccg
     3361 caaagctggg gcattgtcgg tacgccttcc gcattctgga tgttcgtgaa aatggcggcg
     3421 gtgctgtact ggtatctgtg gattcgtgca accttcccac gctaccgcta cgaccaaatc
     3481 atgcgtttgg gctggaaagt gctgattccg atcggcttcg cctacatcgt ggttttgggc
     3541 gtgtggatga tttcaccgct gaatttgtgg aaataagttt cagacggcat cttgaggccg
     3601 tctgaacaaa gcgattttga atacctaacg aaatccctgt tttgagggaa cataatatgg
     3661 ctaacttagt aaaaaccttt ctgcttggcg aactggtcaa aggcatgggc gtaacgctca
     3721 aaaacttttt tgcccgcaaa gacacaattt attttcccga agagaaaacg ccgcaatccg
     3781 tgcgtttccg cggtctgcac gcgcaacgcc gttatccgaa cggagaggaa cgctgcatcg
     3841 cgtgtaaatt gtgcgaggcg gtgtgtccgg caatggcgat caacatcgaa tcggaagaac
     3901 gcgaagacgg cacccgccgc accaagcgtt acgacatcga cctgaccaag tgcatcttct
     3961 gcggtttctg cgaagaggcc tgcccgaccg atgcgattgt ggaaacccat atttttgaat
     4021 accacggcga gaaaaaaggc gacttgcata tgaccaagcc catcctcttg gcaatcggcg
     4081 acaaatacga agccgaaatc gccaaacgca aagccgctga cgcgccgtac cgctaaggag
     4141 cagacgaatg actttttccg tgattctgtt ctatatcctt gccgccatcg ttttgtacgg
     4201 tgcggttcgt accgttaccg ccaaaaaccc tgtccatgcc gctttgcatc tggtgctgac
     4261 cttctgcgtg agcgcgatga tttggatgct gatgcaggcg gagtttttgg gcgtgacgct
     4321 ggtggtggtt tacgtcggcg ccgtaatggt gttgttcctg ttcgtcgtga tgatgctgaa
     4381 tatcgacatc gaggaaatgc gcgccggttt ctggcggcac gcgcctgttg ccggtgtggt
     4441 cggtacgttg ttggcggttg ccctgatcct gattctggtc aacccgaaaa ccgacctggc
     4501 cgcatttggt ctgatgaaag acattccggc cgattacaac aatatccgcg atttgggcag
     4561 ccgtatttat accgactacc tgttgccgtt tgaattggcg gcggtattgc tgctgttggg
     4621 catggtggcc gcgattgcat tggttcaccg caaaaccacc aatccgaaac gtatggatcc
     4681 agccgaccaa gttaaagtac gcgccgatca aggccgtatg cgtctggtga aaatggaagc
     4741 ggtcaaaccg caagtcgaat ctgccgaaga aagcgaagtt tcagacggcc tcaagacgga
     4801 aggggagggc aaagcatgat tactttgacg cattatctgg tattgggcgc tctcctgttc
     4861 ggtatcagcg cgatgggcat ctttatgaac cgcaaaaacg tgctggtatt gctgatgtcc
     4921 atcgagctga tgcttttggc ggtgaacttc aactttatcg ccttctcgca acatttgggc
     4981 gatactgccg gacaaatttt cgtattcttc gtattgaccg ttgccgctgc cgaatctgcc
     5041 atcggtttgg cgattatggt gctggtgtac cgcaaccgac aaacgattaa tgttgccgat
     5101 ttggacgagt tgaaagggta atcatgaacg atatgacttt atatttgata attgcccttg
     5161 ttccgttggc aggctcgctg attgcgggtt tgttcggcaa caaaatcggg cgtgccggcg
     5221 cgcatacggt tacgatactc ggtgtcgctg tatctgccgt gctgtcggct tatgtgctgt
     5281 ggggattcct caatggcagc cgtaccaagt ttgacgaaaa cgtctatacc tggctgacaa
     5341 tgggcggctt ggatttttcc gtcggcttct tggtcgatac gatgacggcg atgatgatgg
     5401 tcgtggtaac gggcgtgtcg ttgatggtgc atatctatac catcggttat atgcacgatg
     5461 aaaaagtcgg ctaccaacgc ttcttcagct atatttcttt gtttacattc agcatgttga
     5521 tgctgattat gagcaacaac ttcatccagc tcttcttcgg ttgggaagcg gtgggcttgg
     5581 tgtcgtatct cttgatcggt ttctatttca aacgcccggg cgcgacattt gccaacctga
     5641 aagccttttt gatcaaccgt gtcggcgact tcggcttttt gctcggtatc ggcttggtgc
     5701 ttgcctattt cggcggcagc ctgcgctatc aggacgtatt cgcctatctg cccaacgtac
     5761 aaaatgccac catccaactg tttcccggtg tggaatggtc tttgattacc gtaacctgtt
     5821 tgctcctgtt tgtcggtgcg atgggtaaat cggctcagtt cccgctgcat gtctggctgc
     5881 ccgattcgat ggaaggcccg acgccgattt ccgcattgat tcacgccgca acgatggtta
     5941 cggcgggtct gttcatggtg tcgcgtatgt cgccgattta cgaaatgagc agcactgcgc
     6001 tgtcggtcat tatggttatc ggcgcgatta ccgccctgtt tatgggcttc ttgggcgtga
     6061 ttcaaaacga catcaagcgc gtggttgcgt attccaccct gtcgcaattg ggctacatga
     6121 ccgtggctct gggcgcatcc gcctattccg tggcgatgtt ccacgtcatg acccacgcct
     6181 tctttaaagc cttgctgttc ttggcggcgg gcagtgcgat tatcggtatg caccacgatc
     6241 aagatatgcg ccatatgggc aacctgaaaa aatacatgcc gattacttgg ctgaccatgc
     6301 tgatcggtaa cttgtcgctg atcggtacgc cgttcttctc aggcttctat tccaaagatt
     6361 cgattatcga agcagccaaa tacagcaccc tgccgggcag cggctttgcc tattttgccg
     6421 tcctcgccag cgtgtttgtt actgcgttct acgcgttccg ccaatacttt atggtgttcc
     6481 acggcgaaga gaaatggcgc agcctgcctg agcatcatga cgatcatcat ggcgaagaac
     6541 atcacggctt gggcaaaaac gacaatccgc acgaaagccc gctggtcgtt accctgcctt
     6601 tgattctgct tgcaattccg tccgtcatca tcggctatgt tgccatcgag cctatgcttt
     6661 acggcgattt cttcaaagac gtgattttcg tcaacgccga cgcgcatccg accatgcaca
     6721 tcatgaaaga agagttccac ggcgcattgg cgatggtgtc gcacagtctg cattcgcccg
     6781 tcctctatct cgctaccgca ggcgtattga gcgcatggct tttgtacgtc aaactgccgc
     6841 acctgcccgc taaaatcgcg caggcgttcc gtccggttta cgttttgttt gaaaacaaat
     6901 actacctcga cgccttgtat ttcaacgtct tcgccaaagg cacgcgcgca ttgggaaact
     6961 tcttctggaa agtcggcgat accgccatta tcgacaacgg catcgtcaac ggctccgcca
     7021 aactggtcgg cgcaattgcc gcgcaagtgc gtaaagtcca aaccggcttt atctacacct
     7081 acgccgccgc tatggtgttc ggcgtattgg tattgctcgg catgaccttc tggggattgt
     7141 tccgataagg atgaggcttc agacggcctg cttatcgctg gtgtagaggt cgtctgaaaa
     7201 gaaggtttca ggcggcaata gattagagtt tcagacgaca tctgacattt cagatagtca
     7261 ggccgtctga aaaatagaag ggcggataat atggataaac ggctgtttga cggtacggtt
     7321 cgttttgagc atgtaaccgg tgtcggcacg atgcaggtag atttccagcc caatcagcgt
     7381 gtttatacga tgattggcga gaatggtgtc ggtaagacca aatttttgga atgtttgttt
     7441 acggtattgt tgtttactta tcgaatggcg aacataaaca taggttttag ttggatagat
     7501 ttgcaacgtt tacctttgaa aatagttgaa ttgcctaatg gaaaaaatga ggattttagg
     7561 cgcttttcag agcgtagtaa tcagggcttt aatattaacg aagacgtttt gttctcagga
     7621 tatcaacatc gacttccttt tgtatatttg ccggcacaga gtcgtgggtg ggtaaaaaaa
     7681 caaacttcca aactcagtcg ctgggaagtt ctcaagggcg tgcaagcaaa tatctgggga
     7741 atttgttttc tgtttttcaa tctggccata gttcattctc gcttaaatcc ttaaatatgg
     7801 aaaacgattt agaagaatgg attatccagc gcgcccgatc agccaatccg tatcaggcaa
     7861 acgaagataa tcgaaaaatt gaaattgata ctttactgtt tcttcttaat caatttgatg
     7921 gtcgtattgc acctgatttt ttagaaatta aaggagatga tcgtatattc atcaagttgg
     7981 ataatcaaaa acgcgaattg tcaggattat ccagcgggtt tacgtctatt ttgaaaatta
     8041 tgcaatctat tgttgcggga tacagttact ttaccaatga gcagcaaatt gccaatgtgc
     8101 gcggcgtggt gctgattgac gaaatagaaa gccatttgca caacgaatgg cagatcaaaa
     8161 tcgtgccgct gctgaaaaaa ttgttcccga acacaacgtt tttcattacc acgcattctt
     8221 ctttagtcat cagtcagtta gagcagggag gggcctatcg tttggtacgt agcaaggagg
     8281 acggtgtagt ctatggcaag atgattgact atccaagtaa tgcttctttt gtggatttgc
     8341 tcaacgaagc attcggcgtg gatttgaata agaagaagat agaacgcgcc caagaacgta
     8401 atcaagaaca ggcaaaaaag ccttattgga attggtacgt caggaattgg gcggattagg
     8461 agataaataa tgacggttta tcttttagat accaattact tggtttattt ggcggatgat
     8521 gacagtgacg aagaaaagcg gaaagccgta ttgagcgata tggcggaaaa gttgcagcag
     8581 gatgacaacc gttttgtgat taccccgctt atccgttatg aagttttgcg cggtgtggat
     8641 tggggaaaat ctgaaaaatt gtctcgattg accggtgttt tggcacagtt ttagtctttg
     8701 gacattacac agaatgtttc tgatttggca agtaatctgt accgttttga taagtttgaa
     8761 gccgaacgtg ataacacgcc gaagaattta gaaaaacgta aattcgatat gtttcattat
     8821 gcgactgcca gtgtgaataa tttagaaatc ttatcccacg ataccgatgt taataagatt
     8881 aaagatttac atgaaagaat gaggctggaa gattcggcag aattagcttg agacaaatag
     8941 cagaaacctg aacaaacctt tttcagacgg cctcttaaaa cgtcgtctga caaaccaacc
     9001 cgtacaacga tatttttatt ttaaccacag gttaaccact atgttttcca actacctact
     9061 cagcttggca atatggatac ccatcgccgc aggcgtgctg gttttggcga ccggtaaaga
     9121 cagccgtgcg ccgctggcgc gtgtgcttgc cttcatggga gcgcttgccg gtttcttggt
     9181 aacgctgccc ctgtttaccg gtttcgaccg tttgagcggc ggctatcaat ttaccgagtt
     9241 ccacgagtgg attccgcttc tgaaaatcaa ctacgcattg ggcgtggacg gtatttcagt
     9301 gctctttatc atcttgaatg cgtttattac gctgctggtg gtattggccg gttgggaagt
     9361 cattcagaaa cgtccggcgc agtatatggc ggcattcctg atgatgtcgg gtttgattaa
     9421 cggcgcgttt gccgcgcagg atgcgattct gttttatgtg ttcttcgagg gtatgctgat
     9481 tccgctgtac ctgattatcg gtgtatgggg cggcccgcgc cgcgtctatg cgtcggtcaa
     9541 gctgttcctc tatacgctga cgggttcgct cttgatgttg gttgccatgg tttacctgta
     9601 ctatcagaca ggcagcttct ctattgtcga tttccaaaac atcaaacaga ttccgttggg
     9661 cgtacaacag cttttgtttg tagcgttctt cctgtcattt gccgtaaaag tgccgatgtt
     9721 ccccgtacac acttggctgc cggatgccca cgttgaagcg ccgaccggcg gttcgatggt
     9781 gttggcggca attacgctga aactgggtgc gtatggtttc ttgcgcttta tcctgccgat
     9841 tatgccggac gcggctcgct attttgcccc cgtaatcatc gtattgagcc tgattgccgt
     9901 aatttatatc ggtatggtgg ccctggtgca aaccgatatg aagaaactgg tggcgtattc
     9961 gtccatcagc catatgggtt ttgtaacgct tgggatgttt ttgtttgttg acgggcagtt
    10021 ggacgactgg gcgttgaaag gcgcggtcat tcagatgatt tcgcacggct tcgtgtcggc
    10081 ggcgatgttt atgtgcatcg gtgtgatgta cgaccgcctg cacacgcgca atattgccga
    10141 ttacggcggc gtggtcaatg tgatgcccaa gtttgcggcg tttatgatgt tgttcggtat
    10201 ggcgaacgcc ggcctgcctg cgacttccgg cttcgtgggc gagtttatgg tggttatggg
    10261 cgcggtcaaa gtgaatttct gggtgggcgc gctggcggct atgacgttga tttacggcgc
    10321 gtcttatacc ctgtggatgt acaagcgcgt gattttcggc gcgattcaca atccgcacgt
    10381 tgccgaaatg aaagacatca attgccgcga gtttgcgatt ctggcggttt tggcggttgc
    10441 tgttttgggg atgggcctgt atccgaacgc atttatcgaa gtggtgcatc aggcggcaaa
    10501 cgatttgatt gcccatgtgg cgcaaagcaa gatttgaggt gtgtaaatga attggtcaga
    10561 tttgaattta atgcccgcac tgcccgaagt cgcgctgctg tcgctgctgg tgttattgtt
    10621 gccggcggac ttgtgggcca gtgatgacaa atgccgttgg acgcattacg gcgcgttggc
    10681 aacggtggcg gttacggcgg cggtgcagct tgccgtgtgg gaacagggca gcacgtcttc
    10741 gttcaacggg atgtatattg cggacggtat gtcgcgtttg gcaaaaatgg ttttatatgc
    10801 cttgacgttt gtcctgtttg tctatgccaa gccctacaac caagtgcgcg gtattttcaa
    10861 aggcgagttt tacaccctgt cgctgtttgc cctcttgggc atgagcgtga tggtgagcgc
    10921 ggggcatttt ttaactgcct atatcggttt ggaactcttg tcgctcgccc tttacgccct
    10981 gattgccctg cgccgcgatt ccggttttgc cgccgaagcc gccttgaaat attttgttct
    11041 gggcgcgctg gcatccggcc tgctgctcta cggtatttct atggtttacg gcgcaaccgg
    11101 ttcgctggaa tttgccggcg tgctcgcctc ttccttcaat gaagaagcta acgaatggct
    11161 gttgaaactg ggtctggtgt ttatcgtcgt cgccgttgcg ttcaaactcg gcgcggtacc
    11221 gttccatatg tggatgcccg acgtgtatca cggcgcgccc acttctgtta ccgccttggt
    11281 cggcactgcc ccgaaaatcg ccgccgtcgt tttcgctttc cgcatcctcg ttaccgggct
    11341 gggaaccgtg catcatgact ggtctctgat gtttgccctg cttgccgccg cctcgctgct
    11401 ggtcggcaac cttgccgcca tcatgcagac caatatcaaa cgtatgctcg cctattccac
    11461 cgtatcgcat atgggtttca tcctgttggc gtttatggcg ggcgcggtcg gctttgcggc
    11521 gggcctctat tacgccatta cctacgcgct gatggcggcg gcagggttcg gcgtgttgat
    11581 ggtgttgtcg gacggggaca acgagtgcga aaacatcagc gatttggcag ggttgaacca
    11641 acaccgcgta tggcttgcct ttttgatgct gctggttatg ttctctatgg cgggcattcc
    11701 gccgctgatg ggtttttacg ccaaattcgg cgttattatg gcgcttttga aacaaggcta
    11761 tgtatggttg tcggtgtttg ccgtcgtgat gtcgctggtt ggcgcgttct actacctgcg
    11821 cgtggtcaaa gtcatgtatt ttgacgaatc cggccgcgcc cgtcccgccg ccggcggcaa
    11881 caatgccgca aaatccctct taagcgtcaa cgcgctgctg ttggtgctgt ggggcattat
    11941 gccgcaaacc gttatcgact ggtgcgccaa ggcgttggag aacacgctgt gagccgccga
    12001 aacggcagcc gtgtcagagg ctgccgtttt tgttaagata tgccgttccg caatgcagtt
    12061 cagacggcat cgccgccgac aatgcccaaa cagaaagccc accatgaccg catccatgta
    12121 catccttttg gtcttggcac tcatctttgc caacgccccc ttcctcacga ccagactgtt
    12181 cggcgtggcc gcgctcaagc gcaaacattt cggacaccac ctgatcgagc tggcggcagg
    12241 tttcgcgctg accgcctctc ttgcctacat cctcgaatcc cgtgcgggag cggtacacaa
    12301 tcagggttgg gagttttacg ccaccgtcgt ctgcctgtac ctcattttcg ccttcccgtg
    12361 tttcgtgcgg cggtattttt ggcacacgcg caacagggaa taaacaagca aacaagcata
    12421 ggaatgccgt ctgaaaccct ttcagacggc atttgtttca ttcaagtgca ggccggcatc
    12481 gctgtgccgg cacgtttcag tcggcgatat acgccggttt taatatttgc gtgcgactgc
    12541 aaattctgcc aactgccgca ggcgcagggc tttgtcgccg aagggttcga gcagcgcgac
    12601 tgcttcggca accagtttgt gtgcgtatga gcgcgccgct tccaagccca tcagtttcac
    12661 ataagtcggc ttgtcgttgt cggcatcttt gcccgccgtc ttgcccaaag tcgccgtgtc
    12721 cgcttcacaa tccaacacat cgtcgatgac ttggaacgcc aaacccagtt ttgccgcgta
    12781 agcgtccaat acggcaagtt ccgcgtcgga cagatcggga cacgccgtcg cccccaataa
    12841 aaccgccgca cggatcaacg cccccgtttt caggctgtgc atccgttcca aatcggcttg
    12901 aaccatttgt ttgccgacat ttgccaaatc gattgcctgt ccgcccgcca tacccgcgct
    12961 gccgcccgct tttgccaaca ccgacaacat tgccaactgg cgtgcggcgg gcagttctgt
    13021 cggacggctc aacacgtcga aggcttgggt ttgtaaagcg tcgccggtca gaagggcggt
    13081 ggcttcgcca tatttgatgt ggcaggtcgg tttgccgcgc cgcaggctgt cgttgtccat
    13141 cgccggcata tcgtcgtgaa ccaaagaata gacgtggatc atttcgattg ccgccattgc
    13201 ctgtccgacc gcatccgcca cggccccgcc caattccgaa gccgccagca ccagcatcgg
    13261 gcgcagacgc ttgccgccgt ccaaagccgc atagcgcatc gcttcgtgca gcgtgtgcgg
    13321 gatttcgttt ccagacggca aaaagcgttc cagcagcagc tctgtctgcg cctgcgccct
    13381 ctgttgccac gctttcaaat cattcgtcgg attcaaggtt caactccttc gtcccgtccg
    13441 catccaaaac ctgtaatttc tgttcgactt gggccagctt ggtttggcag tacctgacca
    13501 gctcgttgcc ttcctgatag gcggcaagcg cgtcttccaa gggcatttcg ccctgcatgg
    13561 actgcgtcag cgattcaagg cgcgacaagg cctcttcaaa cgatttcggg gtgtttttct
    13621 tcatagtttc gggtgttccg gtaaaaagcg gattgtagca tttttgcaaa acggatgccg
    13681 tctgaaaccc gaatccggct tcagacggca ttttttccgc cccggtcggc aaggcgttac
    13741 ccgagcagtt cgtcggtgat gccctgcaaa aaggcgaggc gttcggggct tgccgccccg
    13801 gtttcggcgg cggctttgaa ggcgcagccg ggttcggcgc ggtgggtgca gttgtggaag
    13861 cggcattgcc cgacaaggtg gcggaaatcg gggaaatagt gcggcaaatc ggcggcttgg
    13921 aggtggtgta aaccaaattc ttgcaaaccc ggggagtcga tgagttgggt ttcgccgttc
    13981 aaatcataaa gccgggcgtg ggtggtggtg tgttttcccg agtcgagtgc ggcggaaatg
    14041 tcgccggtgc gggcggtttg gctgcctaaa agggcgttgg tcagggtgga tttgcccata
    14101 ccgctttgtc cgagcaggat gttgctgtgc ccttgcagga cggggcgcag gctgtcggcg
    14161 ttttccagca cgcgggtttc gatgacgggg taacccagcg tttcgtagaa tttgagtttt
    14221 tcgagccaga gggcggtttc gggcaggtcg gctttgttca ggacgatgac ggcgcggatg
    14281 ccggcggctt cggcggcaag cagggcgcgc tgtagcagcc gcacgctcgg actcgggacg
    14341 gcggcggtta cgatgaggag ttgggtaacg ttggcggcga tgagtttggt tttccacgcg
    14401 tcttggcggt agagcaggct ttggcgcggt aaaaaatctt caatcacaac ttgttcggcg
    14461 ttgacggggc tgatgcggac gcggtcgccg caggcgaaat cgacgcgttt tttgcgggtg
    14521 ctggcttcgt aggttgtgcc gtcgggcgtg cggacgatgt agcggcggcc gtagccggcg
    14581 gtaatttggg cggtgtcgtt catggtttct ttggggttgg gtgtggaaat gccgtctgaa
    14641 aaggggtgtt cggacggcat cggttcagtc gtgctgccac tcgacgtgtt cgctgaggaa
    14701 gccgccgctc tggtgcgccc agagtttggc gtaaagcccg cgtttttcga ggagttcggc
    14761 gtgtgtgcct tcttcgatga tgcggccttt gtcgaggacg acgagcctgt ccatcgcggc
    14821 aatggtggag aggcggtggg cgatggcgat gacggttttg ccttccatca ttttgtcgag
    14881 gctttcttgg atggcggctt caacttcgga atcgagcgcg ctggtggctt cgtcaagcag
    14941 caggatgggt gcgtctttga gcatcacgcg ggcgatggcg atgcgctggc gttgcccgcc
    15001 ggagagtttc acgccgcgtt cgccgacgtg cgcgtcgtag ccgctccgcc ctttggcatc
    15061 ggaaaggtcg gggatgaagc cggcggcttc ggcgcgttcg gcggcggaaa ccatttcggc
    15121 atcggtcgcg tcggggcggc cgtaaataat gttgtcgcgc acggaacggt gcagcagcga
    15181 ggtatcttgt gtgaccaaac cgatttgggc gcgtaaagat tcttgggtaa cgccgcttat
    15241 gtcctgcccg tcgatcgaaa ccgtgccgct ttgcggttcg tagaaacgca aaagcaggtt
    15301 gacgatggtg gatttgcccg cgccgctgcg tccgatcaag ccgacttttt cgccgggttt
    15361 gatattcagg ttaaagccgt tgagcagcgg tttgccggct tcgtagcaaa aatcgacgtg
    15421 ttcgaacttg attgcgcctt gcggcacgtt cagcggcagg gcttggggct tgtcgaggat
    15481 ggtgtgcggt ttggacaggg tcgccatgcc gtcgttgacg atgccgatgt tttcaaacag
    15541 ccgcgcggat tcccacataa tgtattgcga cagcccgttg gcgcgcaacg ccatcgcggt
    15601 ggcggtggcg accgcaccca cgccgacctg tccgttgtgc caaagccaga tgcccagtgc
    15661 ggcggcggag agggtcagcg aagtgttgac gatgaagctg cacgaatgca gcagagtcgc
    15721 cagccgcatt tgggcgcgca ccgtaaccat aaattcttcc atcgactgct tggcatagac
    15781 ggcttcgcgc gcgccgtgga aaaagagttt gacggtggcg atattggaat aggcatcggt
    15841 aatgcggccg gtcatcagcg agcgggcatt cgcctgccgt gcggcggttt gccccaatct
    15901 gggaatcagc aggcgcatta ccgaagcgaa accgattatc cagccgataa agggcagcag
    15961 cagccatgag tcgagcgagg cgagaatcac gcccgaagtg atgaaataca ccgacacata
    16021 aacgaccata tcggcaaccg tcatcaccgc gtcgcgcaac gccagcgcgg tctgcatgac
    16081 tttggcggac acgcggccgg caaattcgtc ctgataaaaa ccgaggctct ggttcagcat
    16141 caggcggtgg aagttccagc gcaggcgcat agggaacacg ccctgaaggg tttgcaggcg
    16201 cacgttggac gcggcaaacg cccacacgac cgaaaacacc atcatcgccg ccattgccgc
    16261 cagttcccaa cctttttcgg caaacagttc ggcgggcgcg tatttgccga gccactctac
    16321 gattttgccc ataaattgaa aaatcagggc ttccataatg ccgatgccgg cggtcagcgc
    16381 agctagggcg gctatccatt tccgcacccc gtccatactg ctccagacaa accggcacaa
    16441 gcctttttcg ggcgttttcg gcgcggcttc ggggtagggg tcgattcggg actcgaacca
    16501 ggaaaatatt ttgttcaaca ttgttttcga tttcggtaaa acagtttcag acggcatcaa
    16561 acacaatgcc gtccgaaagg agggacaata acgccatttt acgggaaaag ccgtcgggaa
    16621 gacagcgcgg ggcggaaacg cagggtttcg tcagggcaaa cgccgcgccg ccttcggacg
    16681 gcattatttc agcaggtttt tcagggcaag gcgcacgcct tcgcccacgt ccgtcccctt
    16741 cggaacgcct ttgaccgccg cttttgcctc gcgttcgttg taacccagcg caagcagcgt
    16801 gctgacgatg tcttccgttt cgtcggcggc gggtgaggcg gcaaacagcc tgtccgttac
    16861 cgtatgcgcg accagcttgc cgcgcagttc caagaccata cgttcggcgg tttttttgcc
    16921 gattcccgga gcggaggaga ggcgtttgac atcttcttct gcaaccgccc gcgccagctc
    16981 gtctgccgtc atcgccgaca aaatgcccaa agccgttttc acgccgatgc cgccgacctt
    17041 aatcagttgg cggaaggtct tgcgttcttc cgcagtggca aaaccaaata aaagatgtgc
    17101 gtcttcccga atgataagct gggtaaacag ttgtacgctt tcacccacgg gcggcaggtt
    17161 gtagaaggtc tgcatcgata cgtcggcttc ataaccgaca ccgttgacat cgatgacgat
    17221 ttgcggaggg tttttttcaa ccagtttgcc ggtcagtctg ctgatcatgt gtgccgaatc
    17281 ctgaagtgtc gggtgcaaaa tgccgtctga aaccggttcg ggctccagac ggcacggatt
    17341 gtatcaaatt cagtcgtcgc ggcgggagga aatcacgcgg ccggtacggg catcgacaac
    17401 gactttgtat tcctgtccgt ttttgacgat ttcgacatca tagtgcggac ggccgtcgtc
    17461 gtgttcgaga tcgatgtcgg tgattttgcc gccgacacgc gcccaagccg ctttttcggc
    17521 ttgggcgcgg ctgatgattt tgtcttgttt gccgtgttgg tgtgcggcgt gtccgtggtc
    17581 gtcatcgccg tgtccgtcgt ggtgggcgag tgcgggggcg gaaatgccca acagtgcggt
    17641 tgcggcggcg gtcaagagaa ggtgtttgat gttcatattt gcccttgtaa atcaggggtt
    17701 gaaaaatggg gatattaata aggtatcgaa taaccgtcaa ccggaggtca gtaccgcccg
    17761 aaccataccg cgtgcctgcg cttcggcttc ggcggcgcgt tcctgcgagg caaacgggcc
    17821 cattttgaca acgtattcgt aacggcgttt ttcaaccgag aggttcgggc ttgatgacga
    17881 agcggcgaaa ttttgggcgg cttggttcag ataggcttgt gcttcgtgtt ccgtaccgaa
    17941 agatttcaag tcgataaaga tgtctttgtt ttcggcaacc ggtgcggatt ggcccgggac
    18001 gatttgttcg attttgacgt gtgccgtccc ttggctgaca aagcccaatt tttgcgcggc
    18061 ggctttggat acgtcgatga tgcggttgcc gtggaagggg ccgcggtcgt tgacgcggac
    18121 gatgacgctt ttgccgtttt tggtgttggt tacgcgcaca tggctgggga tgggcagggt
    18181 tttgtgggcg gcggtaaagg cgttcatatc gtagcggtct ccgccggaag ttttgcgccc
    18241 gtgaaacctg ccgccgtacc acgaggcgtt gccggtttgc gtgaattcgg cgactttgta
    18301 gctgcggttg gcggaggcgt gcagtttttc cgccttgacc actgcgtcgg cggatgccgt
    18361 ctgaagggag tgtgtgccga acgcggcggt gaggaggaaa agggtttttc gggttaaagt
    18421 caaaatgtgt tccgttcttg agttgaagac ggatgggcat tatgccctac ggacgcgttc
    18481 cgaaccgccg cacgctgcgg cagcggttcg gaatgtgtcc gaacggtttt cagacggcat
    18541 gaacctgcgt tcaaacgccg cctgcgtaac cgtgttgccg ccacgcttca aagagaatca
    18601 cggagacggt gttggaaagg ttcatactcc ggctgccggg ccgcatcggc aggcggattt
    18661 tttgcgcggc gggcaggctg tcgaggatgt cggcaggcag cccgcgcgtt tccggcccga
    18721 acagtaaaac gtcgcctttt tgaaacgcgg tttcatcggg gcgcgccgtg cctttggtgg
    18781 tcagggcgaa aatacgtcgg cctgccagcg acttgaggca gtcgtcgaag ttttcgtgca
    18841 ccgtcaggct ggcgaattcg tggtagtcga gcccggcgcg tttcattttg gcggaatcca
    18901 atgggaagcc gagcggtttg acaaggtgca aatccgcgcc ggtattggcg cacaggcgga
    18961 tgatgttgcc cgtgttcggg gggatttccg gctgatataa aacgatggta agcataaata
    19021 tcaatcactt ataggcgcgt aaccttgcca taaggcggat ggggtgtcaa aaaatttagt
    19081 tattttttca ttggcgtgcg tgccagcgtc cagcagcaga ttcggtttgc gcccgatttt
    19141 ttcagcgtct ttgccaattc gtccagcgtc gcgccggtgg taaagacatc gtcgattaac
    19201 aaaatattac agttttccgg tatcggtgtg cggatttcaa aggcgttttt gatgtttcgc
    19261 cgccgttcgc cgcctttgag cgtgctttgc ggcgggcggt ggtgtcggaa tacggtgtgt
    19321 cggggcagta tctgccagcc gtagcgttgc gccaacaacc cgacgatgct ctcgctttgg
    19381 ttgaacccgc gttgcagcag ccgctccctg cttagcggaa cgggtaggac gaaatcgaaa
    19441 cattcgtctg aaagccggtc gggcggattc tgcatcgtca ggtctgccag cggctgcgcc
    19501 atgcccaaat cagccaagtg cttcagcgcg cgtatcatat tgctgacggg cggttcgtaa
    19561 tgcagcgatg cccacatccg gtcgaatgcg ggcggttttt tctgacatcc gccgcacacc
    19621 gcgccgcctt gaacgtgcct gaaacacagg gggcagctgt ttgccgcgtc ggtgcggtat
    19681 gccgccaaat ccgccgcgca gccggcgcag atgccgtctg aaacgccgga cgaaccgtgg
    19741 cataatacgc aacgcctgat agtgggcgcg tctgcgatgc gccgccaaca agagagaaaa
    19801 ttcatgcctg atgctgccaa aaaagtttac ctgatacacg gttggggggc gaaccgccac
    19861 gcgttcgacg atttgatgcc gcgcctgccc gcaacgtggc cggtgtccgc cgtcgatttg
    19921 cccggacacg gggacgcgcc cttcgcgcaa cccttcgata ttgaggcagc tgccgacggc
    19981 attgccgctc aaatcgatac gtcggccgac attctcggct ggtcactcgg cggattggtc
    20041 gcgctgtatc tggcggcgcg ccatcccgac aaagtccgtt cgctctgcct gacggcgagt
    20101 ttcgcacggc tgacggctgc cgaagactat cccgaagggc ttgccgcgcc tgcactgggc
    20161 aaaatggtcg gcgcgttccg aacggattat gccaaacata tcaaacagtt tctacaactc
    20221 cagcttctgc acacgcctga tgccgccgaa atcatcggca gaatcctgcc cgatttggcg
    20281 cgctgcggca cgcctcaagc cttacaggaa gctttggacg cggcggaaag ggcggatgtg
    20341 cggcatttgt tggacaagat agatgttccg gtactgctgg tgttcggcgg caaagacgcg
    20401 attacaccgc cgcgtatggg cgagtatctg catcggcatt tgaaaggaag caggctggtt
    20461 gtgatggaaa aggcggcaca cgcgccgttt ttgagccatg cggaagtgtt tgccgcgctg
    20521 taccgcgact ttgtcgaagg gggtttgaca tgaatccgca ggacaaatgc tggcaggttc
    20581 accgccatct tgccgaacat accgaccaac gcctgacact cgtccgcaac gcgcccgggc
    20641 atatcctgct tgccggtgcg gatgcggaca tcagccgcag cctgttggcg aagcgctatc
    20701 cgctggcggt atttgaagaa tacgattccc gtgcggattt tttggcggct gccgcagccg
    20761 cgcgcaaggg cggtttctgg caaaaactga cgggcagggg cgtggtgcag cactgccaat
    20821 ccccgaccgt gccgctgccc gaagcgtgtg ccgatatgtt gtggtcgaac ctcggactgt
    20881 tggcggcgga acaaatcctt cccgtgctgc acaactgggc gcgcgcgctg aagacggacg
    20941 ggctgctgtt tttcacctgc ttcgggcgcg ataccttggc ggaactgaaa tcccgtctga
    21001 aagaaaacgg cattgaaagc cgcagcgtga tgtttcccga tatgcacgac ttgggcgata
    21061 tgctcgccga aaacggcttt tacgatccag ttaccgatac ggcgaagctg gtgttggact
    21121 acaaaaaggc ggaaacgttt tgggcggata tggacacgct gggcgtttgg cgggcggtgg
    21181 cgtgggacga cgaaaacgcc gcgcgttcgt gtgccggggc gatatttgaa cgggaaggcg
    21241 gtttgggcat tacgctggaa acggtgtacg gacacgccgt gaaaaaactg gtgctgccgc
    21301 agggggagaa cgtggtgcgt ttttttccga agagatgatg tgcagatgac gtctgaagcc
    21361 gtttccaagt ttcagacggc atttgtctgt gaaaaccgac agaaataaag gaaatgccga
    21421 tgtatagtga attaaattta aaccggtacg gcgttggctc gccttgccgt actatctgta
    21481 ctgtccgcgg cttcgccgcc ttgtcctgat ttaaatttaa tccactatgt gccgatgccg
    21541 gagacgtata ttgcgtctat aacatcagac tgaagcagta cactgcctgc caggttaccc
    21601 gagttgaaga acacggtggc aaaaaaacac atgcgaccct gctggctttg gactggcggg
    21661 gcaacaaacc gcttggggcg gcggagctgg cggatttgaa accgctttac aaagacttta
    21721 tgtattggga acgcggtctg cacatgtata aggcaagtgc cgtcgtgccg acgggatatg
    21781 tacgggttgg gaataccgcg ccgctgtgcg gcgaagacac gcagcggtat gcctcttttt
    21841 ggggcgacgg ctacgacgtg taccgtcagt tgagatggcg gcagataccc gaaaaacaga
    21901 gaaaggcatt caaaaaagcc gccaaaagca aaaataccgt gatgtttgcc ggacgggaat
    21961 acggcatatc caaacagaat ttgagcgatg tttgggatga ttttgaagac gcgatggaac
    22021 tgaaggcgtt tccctgcctg tcttcgctgt ttctgaccaa gtggcataaa aatctatatg
    22081 aatatctgga ggaatatccg tttatcaccc gattgtgtct ggaaaaccac ggtcagaccg
    22141 tgctggattt cagcaatacg cgcataaccg acctttctgt cgatatgacc ggcgtggaat
    22201 cgctttattt gaacgaaggc ttggacagcc tgaacctgaa aggcgaaatc aaggaaaact
    22261 gcaaggtctg taccgccgga aagggcgcgg gtctgatttt ggaagtgggc aaaagcgttc
    22321 ccaaagtgcg gggtttggaa aacctgaccg ccgtcaacgt catgggcatt gccgatttcg
    22381 atatgcaaaa cctgtcggaa acgtatccca agctcaaaac catacggctg tggggcaaac
    22441 cgggcaatat cgccaatttt tccgccgtat cgggatttga ggatttggaa gtgttcactg
    22501 ccgtagattt gttcggtttc ggcgcggacg acatccctca tcccgaccgg ctgccgaagc
    22561 tgcacaggct gtggatgagc agcctgcccg aagaggcggc gaaggcagtg aagaaacttt
    22621 ataagaaacg gaaggaagac gggctggacc tgtggataga aaaagcccga aaacccgaat
    22681 ggctggcgca aaatttcgac aaccccttcc gcgattggga cggcgcggag catatcccga
    22741 aaagccatgc caaaaaagcg gcggagctgt accggaaaac ccgtgccggc gtggtcaaac
    22801 tgctcggcaa tccgcctgaa aacacagggg aggggctggc agaggcggtc aaagcctata
    22861 ccggaggttt caacaaaatg gacaaaaaac actttatcga cacggtagag cgggaagata
    22921 ttgccgaggc gttggaaacg atattggacc tgataccgga tgggtcttgt gccgacaagg
    22981 aaaaactgtt tgaaatattt gataaaaaca ggaattttta aacagaggca atgccgtctg
    23041 aaacttggta acgggcttca gacggcatcc cgttccaaca ccgccaacac cgccgcaccg
    23101 taacgtgcgg ctttttcttc gcctacgccg tatacggcgg caagctccgc caaaccttcc
    23161 ggctgtttgg cggcaatggc gcgcagggcg gctttgctga gaatgcggta gggttcggac
    23221 tgttcgtgtt ttgcggtttc gttgcaccat tgaatcaggg cgcgcatcag gcagcgtttg
    23281 tgtttggcgg tttcatcgat gccgtctgaa aacggacggc agacggcgag gatgtcccgt
    23341 ccgtatttgg cggcgcgtac gctgcccaag ccgtgtacgg cttcgaggtc ggtttcggtt
    23401 tcgggcgtgg cggcaagcat atcggcaagg ctttcgtcgg agaggacggt atgcagggcg
    23461 cagttttccg cccttgcctg ttcataccgc caggcttcga gtttttgacg cagttgttgt
    23521 tcgcgttcgg tttgcggacg gatgacggca tcgtggctaa agtcggcagc gttttggcag
    23581 actttgagta tgccgtgtcc gaaacgttcg gttttggctt cgcccaagcc gtagatgtcg
    23641 tgcagcccgt tgaggttttg cggcattttt tcgacaaggt cgcgcagggt tttgtcgccg
    23701 aaaatcatat aggcggggat gccttcggct tcagcctgtt tcatgcgcca aacgcgcaat
    23761 gcctgccaca ggcgttcttc gcgttcggta cgcagccagt tgtctttgag ggtgcgggtg
    23821 gcgggcttgt cgcgcttgag cgggcgcagc atcacttcgg tttcgccttt gaggactttt
    23881 ttggcggctt cggtcagttg caatgcctga tatcgggcaa tgttgacggt gaggtagccg
    23941 aggctgatgc actggcggat gacgctgcgc cattctttgt cggacaattc cgcgccgatg
    24001 ccgaatgtgg acagttgctc gtgccggttg ccgcgtatcc aatcgtcgct tttgccgcgc
    24061 aaaaggttgg tgatgtaacc ggcggcaaag cgttgtccgg cgcggtacac gcagctgagt
    24121 aatttttgca ccaacaccgt gccgtcaaac cgtacgggcg gatgcaggca gttgtcacaa
    24181 tgtccgcagg gttcggatgc ttcgccgaaa tgtttgagca acagtacgcg gcggcaggcg
    24241 gcggtttcgc agacggaaag catggcatcg agtttttgca tttcgatttg cttttgcacc
    24301 tcgccgctgt tgccttcggc aatccgttcg cgcagcaaca cccaatcgtt caagccgtaa
    24361 cacagccaac tcacggcagg cagcccgtcc cggccggcgc gccctgattc ctgatagaaa
    24421 tgttcgacac tctggggcat atcgagatgg gcgacaaagc gcacgtcggg tttgtctatg
    24481 cccatgccga acgccacggt cgccaccacg ataatattgt cttcatgcgt aaagcggcgt
    24541 tggttttcct cgcgcacgtc catgctcaaa ccggcatgat acggaatcgc gtttaatccg
    24601 ttttcacgca aaaactgcgc cgcatcttca acctttttgc ggcttaggca atacacaatg
    24661 ccgctttgcc ccgtcatttc tttgcggatg aaatccagca gttgtttttt gccgttgttt
    24721 ttttcgataa cctgataata aatattcgtg cggtcaaagc tggagacaaa ttcggacgcg
    24781 tcgtccaagt gcagataatg cttgatgtcg gcgcgcgtgg cggcatcggc ggtggcggtc
    24841 agggcgatgc gcgggatgtt cggatagcgt tcggcaagca taccgagctg ttgatattcg
    24901 gggcggaaat cgtgtcccca ccggctgacg cagtgcgctt cgtcaatggc gaacaggctg
    24961 acggtttgtt ggtcgagaaa acgtaaaaag cggtcggtaa ccaagcgttc cggcgcgaca
    25021 taaagcagct tcagacggcc ttgggcaagc ttgtcggcaa tctcgcgcgc ctcatctgcc
    25081 gatgtgccgc tgttgactgc cgccgcttca atcccggcca catgcaggct ggccacttgg
    25141 tcgttcatca gcgcaatcag cggcgatacg acaaccgcca cgccttcgcg catcagcgcc
    25201 ggaatctggt aacacaaaga cttgcccccg cccgtcggca tcagcaccgt caaactgccg
    25261 ccgcctgcca aagtattgat gacatcctcc tgcctgccgc gaaattcggg atagccgaat
    25321 acttcgtgca aaatctgtct ggcggtgggt cggtgcgtca ttgttccgtg tccggtaagg
    25381 gtgttggtcg gtcggcggca atatgccgtc tgaaatcggg attttagaag agtttgccca
    25441 cttccgcccc aatatcgtcg gcacgcataa acgtttcgcc gatcaggaag gtatgcacgc
    25501 cgcgcgcccg cataaattcc acatccgcct tgcctgtaat gccgctttcg gtaacgacgg
    25561 ttttgccttc cagcgcgggc agcagcgaca gggtttggtc gagggagact tcaaaagtcc
    25621 gcaggttgcg gttgtttacg ccccacaacg gcgtggtcag gttgcggcat ttttccaatt
    25681 cggtttcgtc gtgcagctcg agcaggacgg tcatgcccaa ttcgtgcgcc agcgcctcaa
    25741 agcgttccaa ttgtccctgt tccagtgcgg cggcaatcag caggacggca tccgcccccc
    25801 atgcgcgcgc ctgataaacc tggtattcgt cgatgatgaa gtctttgcgc agcacgggca
    25861 gcaatacggc ttcgcgcgcc tgtttgaggt attcgggcga accttggaaa tagggttcgt
    25921 cggtcagtac cgacaaacac gccgccccgg cgttttcata ggcgcgggca atctcggcag
    25981 ggcggaagtc ggggcggatt aaccctttgc tcgggcttgc ctttttgatt tcggcaatga
    26041 cggcgggcag gtttaggcgg tgtttgccgc gtatcgaatc gatgaagctg cggacgggcg
    26101 cggcttctgc ggcaagtgcg cggatgtgtt cggcgttgac ggcggctttt tgggcggcaa
    26161 cttcctgtgc cttggtggca aggattttat tgaggatgtc ggtcatgtcg tgttccgtat
    26221 ttgtccgggg aaagggggaa tattagcatc aaaccgttaa cgcctgtttg tgcggaagct
    26281 gtcaaaatag gacaggacgg tctgcggcag ccattgcagg tgcagcctgc cgccggtgct
    26341 gctgacaaag ccggcgtgcc cgccgtgtgc aggttggaac agggtaacgg cttcggacgc
    26401 ttcgtctgca cggggcaggg cttcgggcgg caggaagggg tcgttggcgg cattgagcag
    26461 gagcagcggt ttggcaacgt gtttgagcag cggtttgcag gaagtttggc ggtagtagtc
    26521 gtgccggtcg gcaaagccgt gcagcggtgc ggtgaaacgg tcgtcaaact cgcccagtgt
    26581 tttgcaccct gcggcaaatg ccgtctgaaa accttggagc gaacgtgctt tgggtatcag
    26641 tgtgcggagg aagtagcgcg tgtagagcag ccgcgtgatg ccgctgtcga agcggctgcc
    26701 tgccgcctct gcatcaacgg gggcggatac ggcggccgag gcgtgcggca atgccttttt
    26761 gccctgttcg cccaaatatt ttgccggcgc gttgccgccc agcgatacgc cgacggcgta
    26821 tatttcacgg taacgcgcgg cgagcgtgtc caaagcaaag gcgatttcgg cggtatcacc
    26881 caagtggtag aacaccgggg cggtgttcgc tacgccgccg cagctgcgga aatggacgac
    26941 tgcgccgtgc caaccccgat tgcgtaccgc gagcatcagt tcggccgcgt aatggctgcg
    27001 gctgcttcct tccaaaccgt gaaacagcac gaccagcggc gcatcgggcg aaatgccgcc
    27061 tgaaaagtcg taggcggttt tggttttacc cgtgctgtcg ggaatcagct cgcggcggta
    27121 tgcgggtgcg gggtgttgca ggaatttggc ggcaatcgtg tcggcattgc cgttgcggag
    27181 gaaaaagggc gtgtccggcg gtgtcaaaat cataaggtat cggtttttct gttttcagac
    27241 ggcattggtg atgcggcagc ccgtccggcc ggtgcggacg cgggcgatgc gcgcccggat
    27301 ataggcgcgg aaaaagcgtt ttccgaaaaa ggatatcggc atcggtcagc tttccacgcg
    27361 tttgaaatgg cgcggacgga agcccaaagc cgccagagat gcgaaataca gtccgccgcc
    27421 gacggcaatc aggatgcaga gttgccccgc tttccgcatt ccgccggcgt gcgcccattc
    27481 gaacggcagg caagcctgcg ccgcccacag tccgccgcac atcacggcga gcgcgagcag
    27541 cattttcgcc aagaacgccg cccaacccct gccgggccgg taaataccgt gtttgcgcaa
    27601 caggaagaac aacaatccgg cgttgatgca cgcgcccagg ccgatggcga gcgaaagccc
    27661 ggcgtgtttc aacggaccga taaaggcgag gttcatcaac tgcgtgcaga tgagcgtgaa
    27721 gatggcgatt ttgacgggcg ttttgatgtt ttgccgcgca taaaagccgg atgccaacac
    27781 tttgatcata attaaaccga ttaaaccgaa agaataggca atcagcgcgt gttgcgtcat
    27841 ttgtgcgtca aacagcgtga attctcggta cataaacagc gtcgccacca gcgggaacga
    27901 caataccgcc agtccggccg ccgccggcag cgtcagcagc atgcacaggc gcaaacccca
    27961 gtcgagcagg gcggaaaact gttccgtatc ttggtttgcc gagtgtttgg acaaagtcgg
    28021 cagcaaaatt gtaccgagtg cagcccccag cacgccgccg ggcagctcca tcatgcggtc
    28081 ggcgtaatac atccatgaaa cgctgcccga ttgcagataa gacgcgaaaa tcgtgttgat
    28141 aactaaagaa atttgcgcca cgctcacgcc caaaatcgca ggcgccatct gtttcatgac
    28201 gcggttgacc gccgcatctt tgaaattcag tttgggcagt ttcaaaaagc ccagtttcgc
    28261 cagccacggc agttggaaac cgagctgcaa aataccgccg acaaaaaccg cccacgccag
    28321 cgcggtaacg ggcggatcga aatacggcac gaaaaacagt gcgaatacga taaaagagat
    28381 gtttaaaaac gtgggcgtaa acgcgggaat gccgaactta tggtaggaat tgagtatcga
    28441 tccgacaaaa gaagacaaag aaatcaataa tatataagga aacgtaatcc gcagcaggct
    28501 gatggaaagt tggaacttgt ccgcgtcttt ggtaaagccg ggcgcggaaa cataaatcac
    28561 ccaaggcgcg gcaagtatgc ccagcgcggt aacgacgatc agcacaaacg acagcattcc
    28621 cgcaacgtgg cggataaaag cctccgtcgc ctctttagaa cgcgtttcct tatattccgc
    28681 caaaatcggc acaaacgctt gggcaaacgc cccctccgca aacacgcggc gaagcaggtt
    28741 gggcagtttg aacgcgacaa aaaacgcatc cgtcaccata cccgcgccga atgcccgcgc
    28801 aatgaccgta tcgcgcacaa atcccaaaac gcgcgacacc atcgtcaggc tgccgacttt
    28861 tgccaaagct ccaagcatat tcatcgtgtt tccttttcgg ctgaaacccc gccctttagg
    28921 gcggcaggat cagactttat agtggattaa caaaaatcag gacaaggcgg cgaagccgca
    28981 gacagtacaa atagtacggc aaggcgaggc aacgccgtac cggtttttgt taatccacta
    29041 tatttgaaag gggtgtaacc cttccaaatc agggcaacgc atagggcggt gctttatgtg
    29101 ccgtcctgtg tgttgaaaca tcattgttcc ccaacagccg tatccgtccg gggcggcgcg
    29161 tattgtacga cagaaaccgc ttcggatggc atcgggtttg atgccgtctg aagcggtttc
    29221 ccgaaacgaa aacgtccttt ttcggcggca aactgtatca acacgcgaaa atacaataaa
    29281 ataccaatat ccgatttatt tccaacatca cggagagccg tatgaaatcc agacacctcg
    29341 ccctcggcgt tgccgccctg ttcgcccttg ccgcgtgcga cagcaaagtc caaaccagcg
    29401 tccccgccga cagcgcgcct gccgcttcgg cagccgccgc cccggcagga ctggtcgaag
    29461 ggcaaaacta caccgtcctt gccaacccga ttccccaaca gcaggcaggc aaggttgaag
    29521 tgcttgagtt tttcggctat ttttgtccgc actgcgcccg cctcgaacct gttttgagca
    29581 aacacgccaa gtcttttaaa gacgatatgt acctgcgtac cgaacacgtc gtctggcaga
    29641 aagaaatgct gccgctggca cgcctcgccg ccgccgtcga tatggctgcc gccgaaagca
    29701 aagatgtggc gaacagccat attttcgatg cgatggtcaa ccaaaaaatc aagctgcaag
    29761 agccggaagt cctcaaaaaa tggctgggcg aacaaaccgc ctttgacggc aaaaaagtcc
    29821 ttgccgccta cgaatccccc gaaagtcagg cgcgcgccgg caaaatgcag gagctgaccg
    29881 aaaccttcca aatcgacggt acgcccacgg ttatcgtcgg cggcaaatat aaagtcgaat
    29941 ttgccgactg ggagtccggt atgaacacca tcgacctttt ggcggacaaa gtacgtgaag
    30001 aacaaaaagc cgcgcagtag gcccgtttga aaaatgccgt ctgaaacttg gttttcagac
    30061 ggcattttga ttgggtttag aacgtaaagc ccgtttccag ttcttcatcg ccgaccagtt
    30121 cgaccaagag cgcgtagagc ggggcgagtt cggcataacg gcgcgatacg cggcgcagat
    30181 agtttaagaa acgcgggatt tccggacggt atttgtcttt gccgtcgcgg tagtacaggc
    30241 gtgcgaagat gcctgcaacc ttcaagtgcc gctgcacgcc catccattcg aaccggcggt
    30301 aaaactcgtc aaactcggca gggacaggca agccggcagc ccgcgccttt tcccagtagc
    30361 ggataaccaa atccaaaaca aactcttctt cccactcgat aaaggcatcg cgcaacagcg
    30421 acaccaaatc gtaggaaatc gggccgtaaa gcgcgtcttg gaagtctaaa acgcccggcc
    30481 tgccgcgcgt cagcatcagg ttgcggacga taaagtcgcg gtgcacgtag actttgggct
    30541 gcgccaacaa gggcggcagc agcgtatcgg cggtttgctg ccaaagttgg cgttgtttga
    30601 atgttaattc gcgccccaat tcttttgcga caaaccattc cgggaacagg ttgatttcgc
    30661 gcagcatcgt ttcacggtca tattcgggca aaaccccttc acggcttgcc ttctgcaatc
    30721 cgaccagctc gccgatggcc tccagcaaca gggctttgtg tgccgcttcg ccctgctcct
    30781 gaagcatcgc ggtcaaaaac gtcgtattgc ccaagtcgtt caataccaca aaccccaaat
    30841 ccgtgtccgc gtgcaatacc tgcggcacat tgaccatgtc aaacagcttc tgcaccttca
    30901 aataaggcgc gacactcatc ttgtcgggcg gcgcatccat acagacgacg ctgccgccgt
    30961 ctgaaaacgc cgcacggaaa tagcggcgga aatcagcatc cgccgccgcg aaggacagat
    31021 cgaagtcccg ttcgggataa acggtctgaa gccaattttt cagttcggtt tgccgttgca
    31081 taacagtatc cgggcatttc gggttacaat aaacactatt ctaaccggta aaccgacttg
    31141 aggggcgatt ttggctcgtt tattttcact caaaccactg gtgctggcat tgggcttctg
    31201 tttcggcacg cattgcgccg ccgataccgt tgcggcggaa gaggcggacg ggcgtgtcgc
    31261 agaaggcggt gcgcagggcg cgtccgaatc cgcacaagct tccgatttga ccctcggttc
    31321 gacctgcctg ttttgcagta acgaaagcgg cagccccgag agaaccgaag ccgccgtcca
    31381 aggcagcggc gaagcatccg tccccgaaga ctatacgcgc attgttgccg acaggatgga
    31441 aggacagtcg caggttaagg tgcgcgcgga aggaagcgtt atcatcgaac gggacggcgc
    31501 agtcctcaat accgattggg cggattacga ccagtcgggc gacaccgtta ccgtaggcga
    31561 ccggttcgcc ctccaacagg acggtacgct gattcggggc gaaaccctga cctacaatct
    31621 cgatcagcag accggcgaag cgcacaacgt ccgtatggaa accgaacaag gcggacggcg
    31681 gctgcaaagc gtcagccgca ccgccgaaat gttgggcgaa gggcgttaca aactgacgga
    31741 aacccaattc aacacctgtt ccgccggaga tgccggctgg tatgtcaagg ccgcctctgt
    31801 cgaagccgat cggggaaaag gcataggcgt tgccaaacac gccgccttcg tgttcggcgg
    31861 cgtccccctt ttctatacgc cttgggcgga cttcccgctt gacggcaacc gcaaaagcgg
    31921 actgctcgtc ccgtccgtat ctgccggttc ggacggcgtt tccctttccg tcccctatta
    31981 tttcaacctt gcccccaact tcgatgccac tttcgccccc ggcattatcg gcgaacgcgg
    32041 cgcgacgttt gacggacaaa tccgttacct gcgtcccgat tacagcggac agaccgacct
    32101 gacctggttg ccgcacgata agaaaagcgg caggaacaac cgctatcagg caaaatggca
    32161 gcaccggcac gacatttccg acacgcttca ggcgggtgtc gatttcaacc aagtctccga
    32221 cagcggctac taccgcgact tttacggcgg cgaagaaatc gccggcaacg tcaacctcaa
    32281 ccgccgcgta tggctggatt atggcggcag ggcggcggga ggcagcctga atgccggcct
    32341 ttcggttcag aaataccaga cgctggcaaa ccaaagcggc tacaaagacg aaccttacgc
    32401 catcatgccc cgcctttctg ccgattggca taaaaacgca ggcagggcgc aaatcggcgt
    32461 gtccgcacaa tttacccgct tcagccacga cggccgccaa gacggcagcc gactggtcgt
    32521 gtatcccggt atcaaatggg atttcagcaa cagctggggc tacgtccgcc ccaaactcgg
    32581 gctgcacgcc acttattaca gcctcgacag tttcggcggc aaagcatccc gcagcgtcgg
    32641 gcgcgttttg cccgttgtca atatcgacgg cggcacaacc ttcgaacgca atacgcgcct
    32701 gttcggcggc ggagtcgtgc aaaccatcga gccgcgcctg ttctacaact atattcctgc
    32761 caaatctcaa aacgacctgc ccaatttcga ttcgtcggaa agcagcttcg gctacgggca
    32821 gcttttccgc gaaaacctct attacggcaa cgaccgcatc aacgccgcca acagcctttc
    32881 caccgccgtg cagagccgta ttttggacgg cgcgacgggg gaggagcgtt tccgcgccgg
    32941 tatcggtcag aaattctatt tcaaggatga tgcggtgatg cttgacggca gcgtcggcaa
    33001 aaatccgcgc agccgttccg actgggtggc attcgcctcc ggcggcatag gcgggcgttt
    33061 caccctcgac agcagcatcc actacaacca aaacgacaaa cgcgccgaac attacgccgt
    33121 cggcgcaggc taccgccccg cccccggaaa agtgttgaac gcccgctaca aatacgggcg
    33181 caacgaaaaa atctacctgc aggcggacgg ttcctatttt tacgacaaac tcagccagct
    33241 cgacctgtcc gcacaatggc cgctgacgcg caacctgtct gccgtcgtcc gctacaacta
    33301 cggttttgaa gccaaaaaac cgatagaaat gcttgccggt gcagaataca aaagcagttg
    33361 cggctgctgg ggcgcgggcg tgtacgccca acgctacgtt accggcgaaa acacctacaa
    33421 aaacgccgtc tttttttcac ttcagttgaa agacctcagc agcgtcggca gaaaccccgc
    33481 aggcaggatg gatgtcgccg ttcccggcta catccccgcc cactctcttt ccgccggacg
    33541 caacaaacgg ccctgaccgt cggaaacccg gcaggagcac cgttcctgca caagatagca
    33601 ttccaccgac aaccccaaac ccgccatcaa aggcaggatt caaacgataa ggaaagaatg
    33661 atgaaaatca aagccctgat gattgccgcc gcattgctgg cagcagccga tgtccacgcc
    33721 gcaccgcaaa aggcaaaaac cgcgcccgcc aaagcggtca aagctgccgc cacggcgcaa
    33781 aaagaagccg cacccgcaca acagcagggc ggtatccgct tttcagacgg cattgccgcc
    33841 gttgccgaca acgaagtcat caccaaccgc cggcttgccg aagccgttgc cgaagccaaa
    33901 gccaccctgc ccgaagacgc gcagataagt gaatccgagc tgtcccgaca ggtgctgatg
    33961 cagcttgtca accaatccct gattgtacag gcgggcaaac gccgcaacat tcaggcaagt
    34021 gaagcggaaa tcgatgccgt cgtcgcacaa aatcccgccc tcaaaaacct cagccccacc
    34081 caacgccgcg agcttgccga caacatcatt gccgaaaaag tccgccagca ggcagtgatg
    34141 caaaacagcc gcgtgagcga agctgaaatt gatgccttcc tcgagcaggc gcaaaaacaa
    34201 ggcatcaccc tgcccgaagg cgcaccgttg cgccaatacc gcgcccaaca catcctgatt
    34261 aaagccgaca gcaaaaacgc cgccgtcggc gcggaaagta ccatccgcaa aatctacgac
    34321 caggcgcgca acggcacgga tttcgcaggt ttggcacgcc gatattccca agacgcaagc
    34381 gcgggcaatg gcggcgactt gggctggttt gccgacggcg taatggttcc cgcctttgaa
    34441 gaagccgtcc acgcgctcaa acccggacag gtcggcgcgc ctgtccgcac ccaattcggc
    34501 tggcacatca tcaaattgaa cgaagtgcgc gatgccggca cgccggagga acgtatccgc
    34561 aattccgtgc ggcaatacat cttccaacaa aaagccggac aggcaaccgt caacctgttg
    34621 cgcgacctgc attccggcgc gtatgtcgac atccgctaag gcggtttgaa tccaaaagtc
    34681 ataccgatcg ggcaaaagtc cgggcggtat ggcttttttg aatttcgagt tacttttaca
    34741 ccgccattcg tcattcccgc gcaggcggga atctagcaac gaaaagtaac aggaatttat
    34801 cgggaatggc cggagtttaa aggtctggat tcccgccttc gcgggaatga cgattcagaa
    34861 gttacacgaa actcaaaaaa aaaacgaaac cgaacggacc ggattcccgc ttttacggga
    34921 atgacggcat atcggtttcc gtgaggacaa acctagattc ccgcctgcgc gggaatgacg
    34981 ggattttaga ttgcgggtat ttatcgggaa tggcgattta aaaattacct aaaactaaac
    35041 ttaaaagcaa ctgaaactta aaaaactaaa atttcgcctg cgtgggaagg atgggatttc
    35101 agatttctgt ttttgtggga atttgccgta tcgggcttca gacggcattg cctgccgttt
    35161 tgcccccggg tgcggctgcc ttgatgtagt tgagcgagac aaactgcttc tcggcatcca
    35221 attcggtgat tttaaacaat gcctgcgatt tgggcagcgc gtcaaacggg atgccgttgg
    35281 cgcgcgttgt cagtggcaga ccttcgatgc ggacgaggtc ttctttcaga atcgtcgcgg
    35341 tcagttcgct gatgccttgt tgttgcaggt acacgaggct ccagtaggct tccatctgcc
    35401 gttggaagtc ggcgtaggcg gcgtaggcgg tgtcgaaatc gcgcagggcg gcgaagagcg
    35461 cggcatcgct tttttgatac agcggctcgg cagtgtcgtc gatcaggctg atcagctgct
    35521 tttggttgat gtagtcggcg gcgcggcgca gcggcgaggt aaaccagccg taatgctgca
    35581 cgcccatgcc gatgtgcggc tcggattggg tgctcatgcg cactttgcct gccggttgga
    35641 cgcggaaaag gccgggcagc ccgttttcgt ctagcatttg cgcccaagtg ctgttggcaa
    35701 gaatcatcat ctcgctgacc agcgtatcga tgggcgaacc gcgttcgcgg cggacgacgg
    35761 agacgttgcc ttcttcatcc aattcgatgc tgtaatcgta ttgcggcgcg cggtcgggtt
    35821 cgtatttgcc gcgcgctttt tgcagggcgg tggcgaattg atagaaccaa atcaggtctt
    35881 gatggtgggc gaacatcatt tcgcctgctt ggtccaagcc ggtttctgcg ttgaaatgcg
    35941 gctcgatggc ttggatacgc aggtttgcgg cgatgttgac ggcttcgatt ttgcaggtcg
    36001 gctcgccgac gttgaactcg ccgtccacat cgaaataaat gctgacggca gggcggtatg
    36061 cgcctgcatc gaggctgaac gcggtaatcc agttttcggg cagcatcgtg attttgccgc
    36121 cggggaaata aaccgtactc aaccgctgca taatgatttg ttccattccg ccgccttgtc
    36181 ggacggcaag cgacggcgcg gcgatgtgga tgccgacgcg cttcgtgccg ttgcccaaat
    36241 cggtcaggct caaggcatcg tccacttcgg tggtcgattc gtcgtcaatg gaaaaggcgg
    36301 taacgtcggc cttgggcagg tcgggcattt cgggaaggga gaggtcgggg aaacctgttc
    36361 ctttggggaa gtatttgatt tcaaagccgt cttgcaggta ttgcggaatg gacgtaatgc
    36421 cgcccgtttt tttcgccagt tcgtaggcgg aggttttcag tgcgtcggcg gctttggtaa
    36481 aggctttgta ggtcagcgac tgtttgtcgg gcgcgtgtaa gatggttctc aaatccgccg
    36541 cgatttcaga cggcatctcg ccgcgtttca aggcttccgc ccaagcgtcg atttgcgcgt
    36601 cttgctgttt tttgcgttcg atggcggcaa gcgcttgttt caaagtttct tcgggcgcgg
    36661 ctttgaacac gcctttggct tttttgtaga aatacaccgg cgcggcgtac agcgcaatca
    36721 aagtcgccgc cagctcggtt ttggtcggcg catggccgta atattcttcg gcgatggctt
    36781 cggcggtaaa ctccccttcg ccgcatactt cccacaataa atcggtgtcg atgtccgccg
    36841 cctgcgcctg cgcgttttcc aaaaacgccg ccatatcgcc gtcaaactcg gcaaagacat
    36901 tgtttgcctt cactttggtg cgtttgccgt gttgggtatc gacttggtag gtagcgtcgt
    36961 ttttttggac gacgacggca actttgaact ggccggactc ttcgtaaaaa atattcattt
    37021 ttcggatttt tctgtggaaa ctcaagcggg cgattttagc agattaccga aaatgccgtc
    37081 tgaaaaaagg ttgggggaag gcgggtgcgg ctttgcggtg cttgcgttgc gtttttgacg
    37141 gggtacaatc gccgtttttg aacggaggac ggaatggaga atgtaaaccg cgtgccggag
    37201 caggcacgtt atgatgccga acgcaggcag gcagacgaag cattggcgga cgtgtttccg
    37261 gcagtcagta ttttcggcag cgcgcgcacg ccgcagaatc atacggatta tgcgtttgcc
    37321 tgccgtctgg cgcggcggct gtcggattcg ggtattgccg tcatttcggg cggcgggccg
    37381 gggattatgg aggcggcaaa caagggcgcg tttgccggga agtcggtttc ggtagggctg
    37441 aacattgccc tgccgcacga gcagaaaccg aatccgtatc aggacatcgc cttgcggttt
    37501 tcccgttttg ccgaacgcaa ggcagtgttt ttccgctatt cccaagcata tgtcgtgatg
    37561 ccgggcggct tcgggacgct ggacgaattg tttgaaatcc tgaccttggt gcagacgggc
    37621 aaagtgccgc cgcgtccggt cgttttggtt ggaaaggcgt tttggtcggg cttggcggag
    37681 tggataaacg cgcagctttt ggcgcgcggt atgatttccg aaggggccgc ttctttgttc
    37741 tccatatcgg acgacgaaga cgaaatcatt gcgtatctgt cggaacacgg gcttcagacg
    37801 gcatagcgtc ctgagagtga tgtataattg caaacaattt gtcaattttt gatgtctttt
    37861 ccgaacagga tgccgatatg atcaacccca tcgcctcgct ttccccccta gacggccgtt
    37921 atgcccaatc cgttgaagca ttgcgcccga ttttttcaga gtacggactg atgaaggcgc
    37981 gcgtcaaagt cgaattaaac tggctcaaag ccctggccgc cgagccggag attgccgaag
    38041 tgccgccctt cagtgccgaa acgcttgccg aaatcgacaa agtcatcgaa aacttttcat
    38101 tagaagacgc ggccgccgtc aaagccatcg aagccacgac caatcatgat gtcaaagcca
    38161 tcgaatattg gctgaaaaaa cgttttgccg aagtgccgga agtcgccgcc gtgagcgagt
    38221 tcatccactt cgcctgcacc agcgaagaca tcaacaacct gtcccacgcc ctgatgctgc
    38281 aagaggcgcg cgaaaccgtc atcctgccca aacttgccga aatcatcggc aaactcaccg
    38341 ctatggcgca cgaccttgcc gccgtcccga tgatgagccg cacccacggc cagcccgcca
    38401 caccgaccac tttgggcaaa gaaaccgcca atgtcgtgta ccgcctgcaa cgccagttca
    38461 aaatccttca ggcgcaagaa ttcctcggca aaatcaacgg cgcggtcggc aactacaacg
    38521 cccacatggc cgcctatccc gatgtggatt gggaaacaca ctgccgcaac ttcgtcgaaa
    38581 tcagcctcgg tctgaccttc aacccctaca ccatccaaat cgagccgcac gactatatgg
    38641 cggagttttt ccaaaccctc agccgcatca acacgattct catcgacttc agccgcgacg
    38701 tttggggtta tatttcattg ggttatttca aacaaaaagt caaagcgggc gaagtcggct
    38761 cttccaccat gccgcacaaa gtcaacccca tcgactttga aaactccgag ggcaacctcg
    38821 gcatggcaaa cgccgtattg ggctttttgg cggaaaaact gcccgtctca cgctggcagc
    38881 gcgatttgac cgacagcacc gtattgcgca atatgggcgt gggcgtgggc tatgccgtgt
    38941 tgggtttcgc cgcccacctg cgcggtctga acaagctcga acccaaccct gccgcgcttg
    39001 ccgccgattt ggatgtcact tgggagctgc tcgccgagcc gattcaaacc gtaatgcgcc
    39061 gttacggtgt cgccaatcct tacgagaagc tgaaagacct gacgcacggt aaagacggca
    39121 ttacgcccga agtgttgaaa ctctttatcg aatcgttgga aattccggca gaagccaaag
    39181 ccaaattgct tgagctgacc cctgcgttgt atgtgggcaa ggctgaagcg ttggcgaaac
    39241 ggatttgagt tcttattgaa accgatgccg tccgaacgcg cgttcagacg gcatttttaa
    39301 gataacggga catacggggc gatatttatg caagctgtcc gatacaggcc tgaaattgac
    39361 ggattgcggg ccgtcgccgt gctatccgtc attattttcc acctgaataa ccgctggctg
    39421 cccggaggat tcctgggggt ggacattttc tttgtcatct cgggattcct cattaccaac
    39481 atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta tacccgcagg
    39541 attaagcgga tttatcctgc ttttattgcg gccgtgtccc tggcttcggt gattgcttct
    39601 caaatcttcc tttacgaaga tttcaactaa atgaggaaaa ccatagagct ttctacggtt
    39661 tttttgtcca atatttattt ggggttccga ttggggtatt tcgatttgag tgccgacgag
    39721 aaccccgtac tgcatatctg gtctttggcg gtagaggaac agtattacct cctgtatcct
    39781 cttttgctga tattctgctg caaaaagaca aaatcgctac gggcgttgcg ccacatcagc
    39841 atcatcctgt tcctgatttt gactgccaca tcgtttttgc cggccgcgtt ttataccgac
    39901 atcctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga gctgttggtg
    39961 ggttcgctgt tggcggttta cgggcaaacg caaaacggca gacggcaaac agaaaatgga
    40021 aaacggcagt tgctttcatt actctgtttc ggcgcattgc ttgtctgcct gttcgtgatc
    40081 gacaaacacg atccgtttat cccgggaata accctgctcc ttccctgcct gctgacggcg
    40141 ctgcttatcc ggagtatgca atacgggaca cttccgaccc gcatcctgtc ggcaagcccc
    40201 atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat ttttatttcc
    40261 ttcgcccatt acattacagg cgacaaacag ctcggattgc ctgccgtatc ggcggttgcc
    40321 gcgttgacgg ccggattttc cctgttgagc tattatttga ttgaacagcc gcttagaaaa
    40381 cggaagatga ccttcaaaaa ggcatttttc tgcctttatc tcgccccgtc cctgatgctt
    40441 gtcggttaca acctgtattc aagagggata ttgaaacagg aacacctccg cccgctgccc
    40501 ggcacgcccg ttgctgcgga aaataatttt ccggaaaccg tcttgaccct cggcgactcg
    40561 cacgccggac acctgcgggg gtttctggat tatgtcggcg gcagggaagg gtggaaagct
    40621 aaaatcctgt ccctcgattc ggagtgtttg gtctgggtgg atgagaagct ggcagacaac
    40681 ccgttgtgcc gaaaataccg ggatgaagtt gaaaaagccg aagccgtttt cattgcccaa
    40741 ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca atccttcctg
    40801 atacccggat tccaagcccg attcagggaa accgtcaaaa ggatagccgc cgtcaaaccc
    40861 gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga ggaaaaattg
    40921 aaaagatttg ctataaacca atatctgcgc cccattcagg ctatgggcga catcggcaag
    40981 agcaatcagg cggtctttga tttggttaaa gatattccca atgtgcattg ggtggacgca
    41041 caaaaatacc tgcctgaaaa cacggtcgaa atacacggac gctatcttta cggcgaccaa
    41101 gaccacctga cctatttcgg ttcttattat atggggcggg aattccacaa acacgaaagc
    41161 ctgctcaagc attcccgagg cggcgcattg cagtagcctg ccttcttgcc ggatattgcc
    41221 tttggcagcc tatgccgctg tttgcccttt gaggcggctt ttattttgcc gttttgaggc
    41281 cggggtcggg aataaccgtt ttttgatgat tttccctccc cggctgtgtc atcaaatccc
    41341 tagttgcctt tccaaactct ccaccagatt gtcatccagt tccaaagcct gcgacaggcg
    41401 ggcgaggaag acggtttctt tccgcgacaa atcggcacag accaaccttg ccgccagata
    41461 ggtctccgcc gccaacgcct catcgttgcc gacggcggcg gcgatgtccc cgatgcttgc
    41521 gggcaggcgg tattcggcgg cgagccatgc ggcagtttcg gggtctgtgc cgctttcctg
    41581 ttcgatagtc cggcgttcgg cttcgtctat catgccgtct gaagcggcgg cggctatcat
    41641 ggtacgcaat acggtgcggc tgtatgtttc ttcggttttt ccggcaggtt ggaaatcgct
    41701 ttgtgttacg gttgcccgcc ctttgttttg ctgccacatc tgatagcccc ggtaggcgag
    41761 gtagcccaaa gcggcggtcg aaccgatttt ggtgatggtt ttgcggtttt taccgttcag
    41821 cagcatggag gcgacaccgg caaccagcgc gcctccgccg aatgaattga gcgggctgcc
    41881 ggagaatgtg ctgccttttt tttgaaccgt gcttaagact tggttgagca accgggtgaa
    41941 gttcatgatt tttcctttct gttgcgggaa gggcggtatg tttaccctat ccttttaaac
    42001 ggtggcaggc cggccaataa ttgttggccg taccgctgcg ttttgatgcg gttgtcgagg
    42061 atggttacgc ggccgtagtc ttgttcggtg cggatgaggc ggccgacggc ctggatgagt
    42121 ttgatgccgg cttcggggac ggtgatttcg atgaaggggt tgccgccgcg ttgttctatc
    42181 caacggtttt gggttttttc gatggggttg tcgggcatgg cgaaggggag tttggcgatg
    42241 atgacttgca cgcaggcggt gccgggcagg tcgagccctt cggcgaagct gtcgagtccg
    42301 aagatgatgc tggctttgcc ttcttctatg gcttggtggt gtttttgcag gaggacggct
    42361 ttgggtaatt cgccttgtac gagcaagagc ggcaggtagt ctccgggcag gtgcagggcg
    42421 acatcctgca tttgtttgcg cgaggaaaac aggacgagtg tgccgatggc ttcggtgggc
    42481 gaaatcagct tgggcagcca ttcgatgacg gcggcggtgt gggcttcggg gtctttgggg
    42541 ctggcgtata tggaggggat gtagagttcg ccctgttttt caaagtcaaa ggggcttttg
    42601 agggcgaggg tggtggtttc gggcagccac agcaggccgg tttggcgcaa aatcaagttg
    42661 aagctgccga gcgattgcag ggtggccgaa gtcagcaccg cgcctgccgc gcgccgccac
    42721 aggctgttgg caaggtggga cgcgctgctg atggggctgg cattgaaaat atagtcgttt
    42781 ttgtcgtcgg cacggcgggc tatccatttc gccaacggct cctcgccctc aatggaaaca
    42841 gtggagagca aatcccaaac cgcgctgatt tgttcgatac gggcgataaa aaggccgaac
    42901 tcgccggtca ggcggtcgat gagcgcaccg tcctgttctt tttcgcggcg tgcggcggaa
    42961 agcgcatcgt tcagcccgac aacgtgtttg agcaggctgc gtgcagcaat ggccgtattg
    43021 gaaacggtgg tttcgaggcc ttcggggatt ttgccgtctt cccacagcca agtcggttcg
    43081 ctgttggttc gtctgtcgtt ttcagacagc cccatactta aagacggctc ttccgccaaa
    43141 tggaattgcc attcgtgcag gctgtcgagc aaggatgcgg cggcttcgtc ggcaaggttg
    43201 gcaagttcgg ctttatcggt aagcgcggca attttgccgg taagctgcgg cagtttttcc
    43261 agcgtccaaa cggcaatatt ccatgaatgt tcggcggcaa aacggctgag ggcttttttg
    43321 ggcaggtggt gcgcttcgtc tatgcaatag aaactgtttt cgggcgcagg cagaatcacg
    43381 ccgccgccca tgctgatgtc ggcaagcaga agatcgtggt tggcaacaac gacatcgacg
    43441 gtttccaaca tatcgcgtgc caggtaaaaa gggcattccg gacggttggg acaggcggct
    43501 ttcaggcagc cgtggcggtc gttggtcact ttgagccaaa tcgcgtcatc gattttttcc
    43561 ggccaagcgt cgcggtcgcc gttgaaccgt cgggcggaaa attcgtcggc gatgtcgcgc
    43621 agcagcttca attcttccgg cttgggtttg ctgtcccaca agacggcggg ggcttcaaag
    43681 ccgagcaggt tttgctgggc gttgctttgc gtcagttgat agagtttgta ggggcagaga
    43741 tagcggccgc gccctttggc aagtgcgaag gtcagttcca aaccgctttt ttcgaccaga
    43801 aacggcaggt cgcggtctac caactgctcc tgcaaggcaa ccgtcgcgct gctcacaatc
    43861 agccgcttgc cgcgtgtttg cgccatgatg ccgcccgcaa gcaggtaggc aagcgatttg
    43921 cccacgccgg tcggcccttc gattacggca atgctttcgc cctcgcgctt gggcggctcg
    43981 ccgccttctt cgcgcgtcaa cgtccgcgaa aaagcgttgg caaccgccgc aatcatttcc
    44041 cgctgcgaag cacgcggacg gaaaccgggc aggtttttgc cgatattttg gtaatggtcg
    44101 cggatggcgt ttttttctaa atcggtgagc atagtatcgg tgggcggatg atttggggtc
    44161 attttaacat tgcgcggatg cggtacggta tcgttgtcgg acggtggttc gggcatatct
    44221 gattattgaa agtaaaaaaa aacggaagat attgggtcat ccgtacaaaa tgccgtctga
    44281 agcgggaaac gggcttcaga cggcattgcc gcgcttatac gccggcgacg gggcgggcgg
    44341 gatagccgtg ggtatcgacc gaacaggcaa accgccaagg cgtgtggacg gtgtcggcgg
    44401 acaggtgggc gagttcggga atgtgccgtt tgataaaagt accgtccggg tcggtacggt
    44461 atgaggcggc ggcaatgtcg gggcaggtgt gccgtgaggc ggcaagccgc cagttgcctt
    44521 ggttgattgc cgcatcgaaa tcggtcagct gccgggcaaa ccatatctcg ccttcgcggc
    44581 gggggaggtt taaaacgtgg cagaaaaaat ccgcgctcaa gcgtctcagg gcggggtgga
    44641 ggctgccggt tttgtgcaaa cagcgcatcg cggcatcgat aatcggaatg ccggtccggc
    44701 cctgctgcca aagcgtcagg cgcagggtgt gttccggatt gccgtctgaa gggtcgtcat
    44761 ccgcgtgctg caaggcaagt tgaaggaaaa aatcgcggcg gatgatgttg tccgcccacg
    44821 cgttcagacg gcgttcgagg ctttcccgcg cgagcaggcg cggcgagatg cagccgacac
    44881 tcaaatacgc gcccatcagc gaagtgtttt tgcgcgaagg gaaatccttt aaaacggagt
    44941 aggaagccgc ctgttcgaga aaccgccgcc actgccgcca agccgccgtt tcgccgccgt
    45001 tttgcggcag gaagatgccg tctgaaagcg cggcaggcgg cggggcgggc aggttttcgg
    45061 ggaagggttg gcggtatgcc gcgaataggt ccgaaccggc ggggggctgc ttggaaaagc
    45121 ggtcgagcca tacttcgcgg tagcggttga aatcggtgtg tgccgtaccg ttgtctggtg
    45181 tcaggtcggt tttgccgaaa acggaacggt cgttgacgaa ggttaacgcg atgccgtgtt
    45241 tgtccaattc gtgccaaagg gcgttgtcgg cgagtttgtc ggcaaaagta tgggattcgt
    45301 cggcgatgac ggcgcggata ttgaggcgga cggcgagccg gacaagcccg gcaggcgatg
    45361 ccgccgtgta gagcgggatg ccgcgccttg ccagcccttg ggcgagttcg gcggcggatt
    45421 ggcggtagaa cgcggcgcgg cgcgggttgt ctgtttcggc atcgtcaatc caaatgccga
    45481 taacgggcga accttcggca acggcggcgc ataaggcggc gttgtcgcgg atgcggaggt
    45541 tttggcggaa ccagacgagc gtgtgcgcgg cgcgcgtgtc cgcataaagg gggagggcgg
    45601 tttcagacgg catttcggca gcctttcctg ctggcgattt tttcgttcag aaaatcgatg
    45661 aaactgcgga ctttcgcact caaaaatgcc ctgtccgcat aaacggcgtt cagccggtcg
    45721 gtcgggacgg cgtatccggg cagcagcctc accagcgggc cgcagcgcaa atcgtgttcc
    45781 gccgcccaaa gcggctgata accgatgcac gcgcccgcct taatcatttc gcgcatcatc
    45841 agcgtgttgt cggtacggat gacgggggtc agttcaagcc ggtatttctt gccgtccgat
    45901 ttgcgggtga ggtcgagttt ctgctggttg gtgtaggtcg gcaggacggc aggcagcccc
    45961 gccacttctt ccggtgtttc cggcacgccg ttgcgcctca ggaagtcggg cgaggcaagc
    46021 agggcaaatt cgatttccgc cagcgggcgc gcaatcagcg aaggggaaag ggtttgggaa
    46081 acgcgcaacg ccaaatccac gccttcggca atcaaatcga cgtggcggtt gtccaaaatc
    46141 agttccaacg ccacttcggg ataacgttcg cggtattccg ccagccagtt gcatatctgg
    46201 ctgccggcaa accacagcgg catcgttacg cgcagcagcc cctgcggttt ttccgtcccc
    46261 ccggcggctt tttgcgcggc atcgtcgagc gtgtcgagcg cgtaactgca ttgccggtag
    46321 tattcttccc cggcttcggt caggctgagg ttgcggctgt tgtggtgcag gagtttggct
    46381 tggacggtgt tttccaagtg actgacgtgt ttgcttgcca ttgcggtgga gatgccgagc
    46441 gcgtcggcgg cgcgggtgaa gccgccgctt tggacgactt ggcggaaaac tttgaggctg
    46501 aacagggtgt ccatattttc ttgtgtggaa aagttgtatc aataaaagca gtatatattt
    46561 gaaaaaggga aacatctata ctctgccgcc tgaaatgaag acaaatatca aaggagcttt
    46621 tatgtccgat tgctgcaacc gtatccaacc tgttttgctt tctgttttgc gtatcgtaac
    46681 cgcctacctg tttttgttgc acggtacgtc gaaaatcttc gccttcccca ttgaaatggg
    46741 cagcggttcg cccggcgggc tgttgctgct tgccggtatt ttagaaattg tcggcggcat
    46801 tttgctggtg ttgggcctgt ttgcgcgccc tgccgcgttt gttttgtccg gccagatggc
    46861 ggttgcctat tttatggcgc acgcttccgg aaatgctttg ttcccgattg ccaacggcgg
    46921 cgagtccgca gtgctgttct gcttcgtatt tctctatatc gcggcagcgg gcggcggagc
    46981 atggtcgctg gacaggctgt ttttcaagcg taaagcctga accggactgc ctaaagtgta
    47041 ttttgttgaa tgtttttgag gaaaagaaat gagccgtcaa tccctgcaac aggctgccga
    47101 aagccgccgt tccgtttatt cgttaaataa aaacctgccc gttggcaaag atgaaatcgt
    47161 ccaaatcgtc gaacacgccg ttttgcacac acctttttcg ttcaattccc aatctgcccg
    47221 cgtggtcgtg ctgtttggcg aagagcatga
//
LOCUS       DAXFZR010000003        42885 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000003 DAXFZR010000000
VERSION     DAXFZR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42885
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.003"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(207..1046)
                     /locus_tag="ACFJAS_000103"
     CDS             complement(207..1046)
                     /locus_tag="ACFJAS_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC3981476.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1331..1406
                     /locus_tag="ACFJAS_000104"
     tRNA            1331..1406
                     /locus_tag="ACFJAS_000104"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1364..1366,aa:Arg,seq:ccg)
     gene            complement(1471..2664)
                     /locus_tag="ACFJAS_000105"
     CDS             complement(1471..2664)
                     /locus_tag="ACFJAS_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC3981477.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARAAVPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRVMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2684..3772)
                     /gene="trmA"
                     /locus_tag="ACFJAS_000106"
     CDS             complement(2684..3772)
                     /gene="trmA"
                     /locus_tag="ACFJAS_000106"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC3981478.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3919..5019
                     /gene="aroC"
                     /locus_tag="ACFJAS_000107"
     CDS             3919..5019
                     /gene="aroC"
                     /locus_tag="ACFJAS_000107"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC3981479.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSDK"
     gene            5110..5535
                     /locus_tag="ACFJAS_000108"
     CDS             5110..5535
                     /locus_tag="ACFJAS_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC3981480.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5609..7594)
                     /locus_tag="ACFJAS_000109"
     CDS             complement(5609..7594)
                     /locus_tag="ACFJAS_000109"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC3981481.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVAELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7661..8209)
                     /locus_tag="ACFJAS_000110"
     CDS             complement(7661..8209)
                     /locus_tag="ACFJAS_000110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC3981482.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQPSGNR"
     gene            8351..9646
                     /gene="serS"
                     /locus_tag="ACFJAS_000111"
     CDS             8351..9646
                     /gene="serS"
                     /locus_tag="ACFJAS_000111"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC3981483.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYEFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHMLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDQNGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9719..10717)
                     /locus_tag="ACFJAS_000112"
     CDS             complement(9719..10717)
                     /locus_tag="ACFJAS_000112"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC3981484.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10842..11918)
                     /gene="prfA"
                     /locus_tag="ACFJAS_000113"
     CDS             complement(10842..11918)
                     /gene="prfA"
                     /locus_tag="ACFJAS_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC3981485.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12008..12778)
                     /locus_tag="ACFJAS_000114"
     CDS             complement(12008..12778)
                     /locus_tag="ACFJAS_000114"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC3981486.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12781..13773)
                     /locus_tag="ACFJAS_000115"
     CDS             complement(12781..13773)
                     /locus_tag="ACFJAS_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC3981487.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13840..14523)
                     /locus_tag="ACFJAS_000116"
     CDS             complement(13840..14523)
                     /locus_tag="ACFJAS_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC3981488.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14713..16047)
                     /gene="glmM"
                     /locus_tag="ACFJAS_000117"
     CDS             complement(14713..16047)
                     /gene="glmM"
                     /locus_tag="ACFJAS_000117"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC3981489.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLAAVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16177..17028)
                     /gene="folP"
                     /locus_tag="ACFJAS_000118"
     CDS             complement(16177..17028)
                     /gene="folP"
                     /locus_tag="ACFJAS_000118"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC3981490.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADFVPPEEEWARVEPVLAEAAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKYQ
                     DVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETGL
                     PLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGVNR"
     gene            complement(17168..18061)
                     /locus_tag="ACFJAS_000119"
     CDS             complement(17168..18061)
                     /locus_tag="ACFJAS_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC3981491.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18152..20254
                     /locus_tag="ACFJAS_000120"
     CDS             18152..20254
                     /locus_tag="ACFJAS_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC3981492.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAIGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPHLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20318..21796)
                     /gene="ubiD"
                     /locus_tag="ACFJAS_000121"
     CDS             complement(20318..21796)
                     /gene="ubiD"
                     /locus_tag="ACFJAS_000121"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC3981493.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGGDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLPHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21816..22331)
                     /locus_tag="ACFJAS_000122"
     CDS             complement(21816..22331)
                     /locus_tag="ACFJAS_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC3981494.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSDQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22338..22655)
                     /locus_tag="ACFJAS_000123"
     CDS             complement(22338..22655)
                     /locus_tag="ACFJAS_000123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981495.1"
                     /translation="MYWYPVLVNLMMLAVFGSSLFAGQTVIEKLARLQQPDLPEKAVR
                     YTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLV
                     LKV"
     gene            22703..22810
                     /locus_tag="ACFJAS_000124"
                     /pseudo
     CDS             22703..22810
                     /locus_tag="ACFJAS_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(22968..23780)
                     /locus_tag="ACFJAS_000125"
     CDS             complement(22968..23780)
                     /locus_tag="ACFJAS_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFC3981496.1"
                     /translation="MPPIICRFHFDIADWRVSGGKMRDMAQWRQWAECPDFADGLPDV
                     RRELPFLPAMRRRRLSKAARLVCDAAWDIASAHPGSPVVYASHDGEMARSFDLWLELL
                     KSHTVSPTSFGLSVHNATAGQWSILRRDMSEQTALAVCADGVETALAEAASLLEEGCG
                     SVLVLAADDPLPEGYAVSATRAPFAYALAMVLTKGTRYSLTLSASDDMPSEAGMLPEA
                     YWSGLEWVRFLLNGSRECRRVYRNREWLWQPASCRLKISAMSAPSTTWYRRY"
     gene            23980..24408
                     /locus_tag="ACFJAS_000126"
                     /pseudo
     CDS             23980..24408
                     /locus_tag="ACFJAS_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
     gene            24446..25175
                     /gene="fabG"
                     /locus_tag="ACFJAS_000127"
                     /pseudo
     CDS             24446..25175
                     /gene="fabG"
                     /locus_tag="ACFJAS_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
     gene            25169..26514
                     /locus_tag="ACFJAS_000128"
                     /pseudo
     CDS             25169..26514
                     /locus_tag="ACFJAS_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714702.1,RefSeq:WP_003777802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase"
     gene            26568..26643
                     /locus_tag="ACFJAS_000129"
     tRNA            26568..26643
                     /locus_tag="ACFJAS_000129"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26601..26603,aa:Ile,seq:cat)
     gene            26718..27476
                     /locus_tag="ACFJAS_000130"
     CDS             26718..27476
                     /locus_tag="ACFJAS_000130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3981497.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            27477..28541
                     /locus_tag="ACFJAS_000131"
     CDS             27477..28541
                     /locus_tag="ACFJAS_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC3981498.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            28775..30112
                     /locus_tag="ACFJAS_000132"
     CDS             28775..30112
                     /locus_tag="ACFJAS_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3981499.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNFLGIL"
     gene            complement(30287..31299)
                     /locus_tag="ACFJAS_000133"
                     /pseudo
     CDS             complement(30287..31299)
                     /locus_tag="ACFJAS_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(31308..32102)
                     /locus_tag="ACFJAS_000134"
     CDS             complement(31308..32102)
                     /locus_tag="ACFJAS_000134"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC3981500.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
     gene            32526..33860
                     /gene="gdhA"
                     /locus_tag="ACFJAS_000135"
     CDS             32526..33860
                     /gene="gdhA"
                     /locus_tag="ACFJAS_000135"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC3981501.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWA
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
     gene            33955..34061
                     /locus_tag="ACFJAS_000136"
                     /pseudo
     CDS             33955..34061
                     /locus_tag="ACFJAS_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(34099..34878)
                     /locus_tag="ACFJAS_000137"
     CDS             complement(34099..34878)
                     /locus_tag="ACFJAS_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC3981502.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            35228..35434
                     /locus_tag="ACFJAS_000138"
                     /pseudo
     CDS             35228..35434
                     /locus_tag="ACFJAS_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            35440..35632
                     /locus_tag="ACFJAS_000139"
                     /pseudo
     CDS             35440..35632
                     /locus_tag="ACFJAS_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35833..37236)
                     /gene="mtrE"
                     /locus_tag="ACFJAS_000140"
     CDS             complement(35833..37236)
                     /gene="mtrE"
                     /locus_tag="ACFJAS_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC3981503.1"
                     /translation="MNTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQNDTSV
                     SSVRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWA
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(37289..40492)
                     /gene="mtrD"
                     /locus_tag="ACFJAS_000141"
     CDS             complement(37289..40492)
                     /gene="mtrD"
                     /locus_tag="ACFJAS_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC3981504.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNV
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMILVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVRHASKAGITGSDDKQY"
     gene            complement(40504..41742)
                     /gene="mtrC"
                     /locus_tag="ACFJAS_000142"
     CDS             complement(40504..41742)
                     /gene="mtrC"
                     /locus_tag="ACFJAS_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC3981505.1"
                     /translation="MAFYASKAMRAAALAAAVALALSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVSADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGAIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVSNDQNILMPGLYVRVLMDQVAADNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWAPSENQA
                     AAPQAGVQTASEAKPASEAK"
     gene            41993..42625
                     /gene="mtrR"
                     /locus_tag="ACFJAS_000143"
     CDS             41993..42625
                     /gene="mtrR"
                     /locus_tag="ACFJAS_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC3981506.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQTAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAADAEGGSWTVFRHTLLHFFERLQSN
                     DIHYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGESFDLGKTAPRIIGIMMDNLENHPCLRRK"
BASE COUNT         9940 a        12239 c        11067 g         9639 t
ORIGIN      
        1 tataaaacaa atgggttaaa ccagtatcca tacatcagct tttttatcat cctacttttt
       61 attcatccga tcgggcagac agatttcaaa gatgaaaagc ctattctatt cacacccatt
      121 tgatgtcatt tccacacgga cgaacaaata aattcccata aaaaaacgga gcagatacct
      181 gccccgtttt ttaatttaat ccgaatttag aattttgcac cggattggtt tgccatatag
      241 tcaacagccg ctttgacttc gtcatcgctc aaacctgcat tgccgccttt ggccggcatc
      301 gcgttaaagc cttcaagggc atgtttgtgc aaggtttctt tgcctttttt gatacgcggt
      361 gcccaatcgt cttttttgcc tatgccggga ataccgggaa tcgaaccgcc gtggcacacc
      421 tgacaggttg cttcgaagac ttttttaccg tcaacgccga ccgcaggggc tgccgcaccc
      481 ttgtcttctg ccttcgcttc tgccggagct gcactatcgg caggagcaga agctgttcct
      541 gaagcggcat tgtcggcagg cgcagcctca tcaggattcg ggaaagaacc gccgctttta
      601 ttcgccatgt aggtaatcgc ccgtttgagt tcctgatcgg tcaggtctgc cgcaccgcct
      661 ttggcaggca tggcgttaaa gccgttcagc gcgtgttgga acaaggtatc gaagccttgc
      721 gcgatacgcg gcgcccagtc gccgttgtgt tccagtttcg gagcgttcgg cacattgctg
      781 tccgccgcgt ggcattggat acagattttg ccgaaaatct gttcgccttg gcgttcgccg
      841 acggggatgc cgtcacccat cgtcaattgt ccgacaggct ggatgcgggt ctgcgttgcc
      901 gcttccgtag tggcatcgac atcgccgaac gatccactgc ccgccagctt aatcaggaaa
      961 taaaggactg caataacaat aacgataccg ctcacaaggg taaacagtgc agagccttgg
     1021 gctttgttgt cgcggagttg tttcatttgg taggcctcgc cgtcaggtta ggttgtgctg
     1081 tagattatag tttggcgtgt taaacacagt taacaatatt ttgctggatt atactgaatt
     1141 cacatggtct ttccaatcgc tatcattgaa aatatgaaaa aatttgccaa tggtatctgt
     1201 acaaaacaaa taatccgttg aaaataccgg attatctcca agaaaactcc ccttgtgccg
     1261 ccatacgccg cctgccggcg caagataacc tttgccaatt tgcagaattt acgttaacct
     1321 tacgttttcc gcacccatag ctcagttgga agagtgtcag tttccgaagc tggaggtcac
     1381 aggttcgatc cctgttgggt gcgccaatta taaagaggcc gtctgaaaga taaatatttt
     1441 tcagacggtc ttttgactta cttcaaattc ttatttcaac acttcggcaa gcgcatcggc
     1501 gacataagtg atatttgatg tattcagccc ggcgacgcac atcctgccgg aatccagcag
     1561 gtaaacggca aattcgtcgc gcaacctgcg gacttgcccc acgctcaacc ccgtgtagcc
     1621 gaacattccg cgctgtttga tgaaataagt gaaatcgcgg tcggggattc gggcggtcaa
     1681 tacgccgtaa agtttctgcc gcatcacacg gatgcggtcg cgcatcatat aaacttcgtt
     1741 ttgccacaag gcgtaaagtt cggagctgtt catcacgtcg gcggcgatat acgcgccgtg
     1801 cgcgggcggg ctggagtaga tgcggcggac ggtgaatttg agttgtccga acaccaaatc
     1861 cgcttcttct ttattggggc aaaccacgct taagccgccg acgcgttcgc cgtagagcga
     1921 caggtttttt gaaaacgaat tgctgacaaa cagcggcaaa tccatttcca ccgctttgcg
     1981 gacggcgtag gcatcgctgt ccaaatcgcc gccgaagcct tggtaggcga tgtccataaa
     2041 cggaatcagt ttgcgcgttt tgatgatctg caacacttcg tcccattgcc gttccgacat
     2101 atccacgccg gtcgggttgt ggcagcaggg atggaggatg aggacgctgt tttcgggcag
     2161 ggtgttgaaa aacgcggtca tttcgtcgaa tttcacgccg acagtggcag ggtcgtaata
     2221 tgggtaagtg ccgacctcga aacctgcgcc ttcaaaaatg ccgcgatggt tgtcccaagt
     2281 cggatcgctg acgtaggcgc gcgcttcggg aaaccagcgg tgcaggaaat ccgccccgac
     2341 tttgagcgcg cccgagccgc ccagagtctg tacggtaacg atgcgtccct gcgcgagcgc
     2401 ggggttgcct ttgccgaaca acaaatgctg caccgcgctg cggtaaacgt ccaaaccttc
     2461 catcggcagg tagggagacg gcgcaggcac agcggcacgg gcggtttcgg cacggcttac
     2521 ggattccaat acgggcattc tgccttcgtc gtcgaaataa atgcctatgc tcaaattgac
     2581 tttttcgggg cgcgggtcgt ttttgaaggt ttcgaccaaa ctcaaaatcg ggtcgccggg
     2641 atagtattcg atgtgtcggt acatagtcct taccttttgc ttttcaaagg attttctttt
     2701 tcaacagtac gccgctttcg atatggtgcg taaacgggaa ttggtcgaac agggcggcac
     2761 gtccgaccgt atgggtttcc gtcaaggtgt ccaaattggc gcgcagcgtt tcgggattgc
     2821 aggaaatgta gatgatgttg tcgaactgcg acaccagctt caaagtttcc tcatcgatgc
     2881 cggcacgcgg cggatcgacg aaaatagtgg aaaatgcgta atccgtcaaa acaataccgc
     2941 tctccttaag gcgtgtaaac tcacgttttc cggtataggc ttcggtaaac tcctcagcag
     3001 acaggcgggc gattttgatg ttgcctatcc agtttgcctc aatattccat tgcgccgcac
     3061 tgacggaggt tttggagatt tcggttgcca aaacctgtcg gaaatatcgg gacagcggca
     3121 gggtaaaatt gccgttgccg caatacagtt cgagcaggtc gccgcccaag ccttccgccg
     3181 cgcggcacgc ccattcgagc attttccggc atacagcggc attcggctgg gtaaagctgc
     3241 cttcgatttg gcggtagtgg aaatcgcggt cgccgacgcg cagggtttcc gtcacatagt
     3301 cctgttttaa gactattttc tgccccctgc tccgcccgat aacggaaata tccaactgtt
     3361 gctgtaacgc ttgcgccgcc ttcatccact catcatcaag ccttttgtgg taaatcatgg
     3421 taaccagcat ttccccgctg agcgtggaca gaaattcgac ggcataccaa tggtttctca
     3481 gttcggcaga ttgggcggca acggcaatca attcgggcat caggcagttg actgcctctg
     3541 aagcagcatc gaaacggtcg caacggatca ggcttgcccc gctagccttc tgcccttttt
     3601 caaacatggc gtaaaacatt tcaccgcctt cgtgccaaat gcggaattcg gcgcgcatgc
     3661 ggtagtgttt gtccggagat tcgtacactt cccactcagg aacatccaaa cctgcaaaaa
     3721 gggttttaag gtagtctttt ttaccttgaa gctgctgcgt gtagtcattc atatcgctat
     3781 cctgaaaaat cagaccggat tataagccga ttttcctcgc cgcacgcaaa ctctttgact
     3841 gccgcccctt caatgacgga cgggcttttg tgctaaaatc cgccatcttt ccacactata
     3901 ccgataaagg gaaaaatcat ggcaggcaac actttcggac aaatcttcac cgtgaccacc
     3961 ttcggcgaaa gccacggcgc gggtttgggc tgcatcatcg acggctgccc gcccggcttg
     4021 gaattaagcg aagcggatat ccaatttgac ctcgaccggc gcaaacccgg caccagccgc
     4081 cacgttaccc aacgccgcga agccgaccaa gtcgaaatcc tctccggcgt attcgaaggc
     4141 aaaaccaccg gcacacccat cgccctccta atccgcaaca ccgaccagcg cagcaaagac
     4201 tacggcaaca tcgccaccgc cttccgcccc ggacacgccg actataccta ttggcacaaa
     4261 tacggtacgc gcgactaccg gggcggcggc aggagttccg cccgcgaaac cgcagcccgc
     4321 gttgccgccg gagccgttgc caaaaaatgg ctgaaagaaa aattcggcac ggaaatcacc
     4381 gcctacgtta cccaagtcgg cgaaaaaaaa atccggtttg aaggcagcga acacatttcc
     4441 caaaatcctt tttttgccgc caaccaaagc caaatcgccg aactggaaca ctatatggac
     4501 ggtgtgcgca aatccttgga ttccgtcggc gcgaaactgc atatcgaagc cgccaacgtc
     4561 cccgtcggtt tgggcgaacc cgttttcgac cgcctcgatg ccgaaatcgc ctacgcgatg
     4621 atgggcatca acgccgtcaa aggcgtggaa atcggcgcag gttttgacag cgtaacgcaa
     4681 cgcggcagcg aacacggcga cgaactgacc ccgcaaggct tcctgtccaa ccattcgggc
     4741 ggcatcctcg gcggcatcag caccgggcaa gacatctgcg tcaatatcgc catcaaaccc
     4801 accagctcca tcgccacacc gcgccgaagc atcgacattc acggcaaccc cgtcgaactc
     4861 gccacccgcg gcaggcacga tccctgcgtc ggcttgcgcg ccgcgccgat tgccgaagct
     4921 atgctcgcct tggttttaat cgaccacgcc ctgcgccatc gtgcgcaaaa tgcggatgtt
     4981 gccgcggata cgcccgatat ttcccgctcg gacaagtaaa aagatttagc caaaacacgg
     5041 gctttatact aaaataccga ccattgccgt gccgtctgaa cgcacgattc ttagaggaat
     5101 acaaccgaaa tgacacaaga aaccgctttg ggcgcggcac tgaaatccgc cgtccaaact
     5161 atgagcaaaa agaaacagac cgaaatgatc gccgaccaca tctacggcaa atacgatgta
     5221 ttcaaacgct tcaaaccgtt ggcgctcggc atcgatcagg atttgattgc cgctttgccg
     5281 caatacgatt ccgcactgat tgcacgcgtc ctcgccaacc actgccgccg tccgcgctat
     5341 ctgaaagcct tggcgcgcgg cggcaaacgt ttcgatttga acaaccgttt caaaggcgaa
     5401 gttacccccg aagaacaggc gattgcgcaa aaccatcctt tcgtgcagca ggctttgcaa
     5461 cagcagtccg cccaagctgc cgccgaaacg ccgtctgttg aagccgaagc agccgaatct
     5521 tccgcagcag aataaatccc caaacgaaat gccgtctgaa aaccgatttg gtttcagacg
     5581 gcattttttc gtatgcggca atcacggctc aaatatcgag ttgcgccgta tcgccttcgc
     5641 gttccatcca agcgcggcgg gcggcggctt cgcctttgcc catcagtttg acgaagatgt
     5701 cgcgcgtctc gtcatccgcg ccttcgggga tttgcacctg caacaggcgg cgggtgtcgg
     5761 ggtgcatggt ggtgtctttg agctggtcgg ggttcatctc gcccaagcct ttgaaacggc
     5821 tgatggaata ggcggtttct ttaacgcctt ctttttgcag ccgctctaaa atgccgtcaa
     5881 gttcgttttg gtcgagggcg tagaatttgc gggcgggttt gcttttgcct tgtgcgttga
     5941 catcgacgcg gaacagcggc ggctgggcga cgtagatgtg tccgtcggcg accagtttgg
     6001 ggaagtggcg gtagaacagg gtcaacagca aaacctgaat atgcgaaccg tccacgtcgg
     6061 catcggacaa gatggctatt ttgccgtagc gcaggccgct taagtcggga tggtcgttga
     6121 tggcgtgcgg atcgacaccg atggcgacgg aaatgtcgtg gatttcggcg ttgccgaaaa
     6181 gctggtcggg gtggacttca aagctattga gcactttgcc gcgcaggggc aggatggctt
     6241 gtgtggcttt gtcgcgggcg agtttggcgg agccgccggc ggaatcgcct tcgacgagga
     6301 agagttcgtt ttcgcggatg tcttcgcttt cacagtcggt cagcttaccg ggcaggatgg
     6361 cgacgccgct gccttttttc ttttcgattt ttttaaccga acgtatccgt gcctgtgcct
     6421 gacggatggc gagttcggcg atttttttgc cgaagtccac gttttggttc agccacaatt
     6481 ccaaaggatc gcccgatacg gcggcgacaa gtttcagcgc gtcgcggttg gtcagcttgt
     6541 ctttggtctg accttggaat tgcgggtcga ggacgcgggc ggagaggacg aaggcggttt
     6601 ttccgaacac gtcgtcgctt tgcactttaa cgccgcgcgg taagaggttg tgcaggttga
     6661 tgaagttgtt gacggcgttg aacacggctt gtttcaagcc tgcttcgtgc gttccgccca
     6721 gcgaggtcgg gataaggttg acgtagcttt cgttggcgca cgagccttct tccagccaag
     6781 tcaaagcaaa cgcggcacct tcgccaatgc tgaaatcgcc gttgtggccg tctgaaatgt
     6841 agttttcgca ggagaacagc ggcacggctt cctgtgcgtc ggcaatcagg tcggtcagat
     6901 agcttttcag accgtcgggg taatgccagg tttgggtgtg cgcttcgtct tcgcctttga
     6961 ccggacgggt cagggaaacg cgcacgcccg gcagcagcac ggctttggcg cgcaatagac
     7021 gctcgagttc gggaatgctg taattcgggc tttcaaaata tttgccgtcc ggccacacgc
     7081 gcacttcggt gccgctgtct ttgacggcgc atttgcccac ttgtgccaac ggttcgacca
     7141 catcgccgcc ggcaaacacg atgcggtgga ctttgccgtc tcgtttgaca ctgacttcaa
     7201 gtcgggtgga aagcgcgttg gtaacggata cgcccacacc gtgcaggccg cctgaaaagg
     7261 catacgcact gccgccgtct tttttgttga acttgccgcc cgcgtgcagg cgggtaaaca
     7321 cgagttcggc tacgggtacg ccttcttcag ggtgcaggcc gacgggaatg ccgcgcccgt
     7381 tgtcgcgcac ggaaagcgaa ccgtcttcat gaatttgcac gtcgatttca gtggcgaaac
     7441 cgcccaacgc ctcgtccgcc gcgttgtcga tgacttcttg gcagatatgt gtcgggctgt
     7501 cggtgcgggt gtacatgccg ggacgttctt tgaccggctc caagcctttg aggacggtga
     7561 tgctggattc gctgtattgg ttgtttttag ccataggaat aatctgaaag taagaaaaac
     7621 aacgctttca gacggcctga aagcgttgcg ttccgttatt ttaccggttg ccggaaggtt
     7681 ggcgggcggc aaagtcttca taactttcca taccgcgcag gaagcgggaa gagagttctt
     7741 tcaacgcccc caaataaacg tcgcgtttga aatcaatcac ttcgtcaacg ggcgcccaat
     7801 attgatgcca acgccagccg tcaaattcgg ggtggcgggt ggcgcgcagg ttgacatcgc
     7861 aatcccggcc ggtcaggcgc aggagatacc aaatctgctt ctgcccgcga taagagccgc
     7921 gccattcgcg gcgcacccag ttgttcggca cgtcgtaacg cagccagtcg cgcgtccgcc
     7981 cgacgatttt gacgtgttgc ggcagaagtc cgacttcttc gtaaagttcg cggtacatcg
     8041 cggtttcggg gctttcgccg ggcttgatgc cgccttgagg aaactgccat gaatgttcgc
     8101 gcacgcgctt accccaaaag acttcgttac gttcgttaat taggataata ccgacattgg
     8161 ggcgatagcc ttccctgtcc aacacggtgt cgccctccgt taaattcaat cttgggattt
     8221 tcccacaaat cggacggttt tgacaaatcg gacggtatgg ttcaagcatg tcgaaacacg
     8281 gacggattcg ggaaaatatc ttaaatttgg tttacaataa tacgtttcaa attaattccg
     8341 gaatcagact atgttagaca tccaattgct ccgcagcaac accgccgccg ttgccgaacg
     8401 gcttgcacgg cgcggttatg aattcgatac cgcgcgtttt gacgcactgg aagagcgacg
     8461 caagtccgtt caggtgaaaa ccgaagaact acaagcctcg cgcaacagca tttccaaaca
     8521 aatcggcgca ctgaaaggtc agggcaaaca tgaagaggcg caggcggcca tggatcaagt
     8581 cgcccaaatc aaaaccgatt tggaacaggc tgccgccgat ttggatgccg ttcaaaaaga
     8641 attggacgca tggttgttga gcatacctaa cctgccgcac gaaagcgtac ctcccggtaa
     8701 agacgaaacc gaaaacgtcg aagtccgcaa agtcggtacg ccgcgcgaat ttgattttga
     8761 aatcaaagat catgtcgatt tgggcgagcc tttgggcttg gatttcgaag gcggcgcgaa
     8821 actttccggc gcacgattta ccgtgatgcg cggacaaatc gcccgtctgc accgcgcctt
     8881 ggcgcaattc atgctcgata cgcacatgct gcaacacggc tacaccgagc attacacgcc
     8941 ttatatcgtt gacgatacga cgctgcaagg cacaggtcag ctgcccaaat ttgcagaaga
     9001 tttgttccac gttacccgcg gcggcgatga aaccaaaacc acgcaatatc tgattccgac
     9061 agccgaagtt accctgacca ataccgttgc cggcagcatt atcccgtccg aacaactgcc
     9121 gctgaagctg accgcgcatt cgccctgttt ccgcagtgaa gcgggatcat acggcaaaga
     9181 cacgcgcggc ctaatccgcc agcaccagtt cgacaaagta gaaatggtac aaatcgttca
     9241 tcccgaaaaa tcatacggcg cgctggaaga aatggtcggc catgccgaaa acatcctgaa
     9301 ggctttggaa ctgccctacc gcgtgattac cctgtgtacc ggcgacatgg gtttcagcgc
     9361 ggccaaaacg tatgacttgg aagtttgggt tcccgcacaa aatacctacc gcgaaatctc
     9421 aagctgctcc aactgcgaag atttccaagc acgccgcatg aaggcgcgtt tcaaagacca
     9481 aaacggcaaa aaccgcttgg tacatacctt aaacggctcc ggtttggcag tcggtcgcac
     9541 tttggtcgcg gttttggaaa accatcaaaa cgccgacggc agcatcaata tccccgccgc
     9601 actgcaaccg tatatgggcg gtgttaccaa gttggaagtg aaataagcct taaccctgaa
     9661 cataaatgcc gtctgaaacc tgtttcagac ggcatttcct ttaaactctt aaaacacatc
     9721 aaccgtcggc acgaaccgca ttgccgcaat cgccggtctg tccgacctcg cggatattgg
     9781 acagcgtaac ttccgaaata ttacccaacg cctcttccgt caaaaatgcc tgatgtccgg
     9841 taaacagtac gttatgacaa gacgacaggc ggcggaacac gtcgtcggta atcacatcgt
     9901 tggatttgtc ttcaaaaaac agctcgcgct cgttctcgta cacatccatg cccaatgcac
     9961 cgattttccg gcatttcaac gcctcgatgg cggcggcgct gtcaatcagc ccgccccggc
    10021 tggtgttgat aatcatcacg ccgtctttca ttttgtcgaa cgccgcttcg ttcagcatat
    10081 agtggttttc cggcgtggcg ggacaatgca gcgtgatgat gtccgatcgg gcatacagct
    10141 cgtccaaatc cacatacctg ccgccgagtt tttccacttc ggggttgcaa aacggatcgt
    10201 aagccagcag gttcataccg aaacccttta aaatccgcat cgttgcgata ccgattttcc
    10261 ccgtgccgat aacgcccgcc gttttgccgt acatattgaa gccggtcaga ccttccagcg
    10321 aaaaattcgc atcgcgggta cgctggtagg ctttgtggat acggcggttc aaagtcagca
    10381 tcagaccgac cgtatgttcc gcaaccgatt cgggcgaata ggcaggcacg cgcacgactt
    10441 tcaagcccaa ctcttcagcc gccttcaaat ccacattatt gaagccggca caacgcaacg
    10501 ccacagtttt cacgccaatt tgcgccaatt tttccaacac gggccggctg gcgtcgtcgt
    10561 ttacaaaaat acaggccgct tccgcgcctt ccgccatttt cgccgttttc gcatccagca
    10621 tgaaatcaaa aaactccagc tcgaagccga aatgccggtt ggcgcgggta aaatgttcgc
    10681 ggtcatagct tttcgtaccg taaatcgcaa tcttcatcaa tatgtccagt tgtcgtctat
    10741 ggttgagaaa cggcataata caccgaattc aaacaaatca gtagaatatg gcggattaaa
    10801 attgatttca tgcacggcat ttccatttca aaacacaaaa ctcaatcgcc catcgccgcc
    10861 agaagctccg cctgatgttc ggcaatcagg gcatcggtga tttctgccaa atcgccgtcc
    10921 atcacaaaat ccagcttgtg cagggtgagg ttgatgcggt ggtctgttac cctgccttgg
    10981 ggatagttgt aggtgcggat gcgttcgctg cggtcgccgc tgccgatgag ggatttgcgt
    11041 tcggcggctt ctttggcttg gacttcgcgt ttttgcgcgt cgttcaggcg ggcggcgagg
    11101 actttcatcg cctgcgcttt gttggcgtgt tggctgcggc cgtcttggca ttcgaccacc
    11161 atgcctgtcg gcaagtgggt aatgcggacg gcggagtcgg ttttgttaat gtgctgaccg
    11221 cccgcgccgg atgcgcggaa ggtatcggtg cgcaggtcgg cggggttcag ttcgatgtct
    11281 tcgagctcgt ccgcttcggg catgacggca acggtacagg cggaggtgtg gatgcggcct
    11341 tggctttcgg tggcggggac gcgctgcacg cggtgtccgc ccgattcaaa tttcagacgg
    11401 ctgtacgcgc cgagtccgac aatacgggcg atgacttctt tatagccgcc caattcgctt
    11461 tcgttggcgg acacgatttc aacctgccag cggttgcgtt cggcgtagcg gctgtacata
    11521 cgcagcaaat cgccggcaaa cagcgcggct tcgccgccgc ccgtgccggc gcgtatttcg
    11581 ataaagatgt ttttgtcgtc gtcggcatct ttgggcagca gcagtttttg caattcggta
    11641 tccagttcgt cgattttggc tttcgccgct tcgatttctt cggcggcaaa gtctttcatt
    11701 tcggggtcgg acaacatttc ttcggcatcc gccaagtcgc tttgggcaag ccgatagttt
    11761 tggaatactt cgacgacggg ggtcagttcg gcgtgttcgc gcgtgagctt gcggtagttg
    11821 tccatatcgg atgtggcttc gggttgccct aaaaggtggg taacttcttc caaccggtcg
    11881 ccgagttgtt gcagtttttc taagatagac ggcttcataa ttcttccata acaaacgccg
    11941 cctgaatttc agacggcatc aaaacgcggt cattataata tattttctga atattcaaaa
    12001 agataatcta gggacggagc agactgtcgg cattgcgttc cgttatcgcg gcgatttccg
    12061 ccgcgccggt accgcgtatt tccgcagcaa tttcggcaat ccgaagaatg ttggcaggcg
    12121 tattgacggt gttttccaac atgaacgggc tatccgtttc caatacgaaa tccccgtcgt
    12181 tcaaggcttt aagcgtatcg cgcactttac gcgcgttcgg attgagcagc agcgaaccga
    12241 tgccgatttt gaaccccagt tttgtcaaca cgcgcgcttc ttccgcgctg cctgagaaag
    12301 cgtgaacgat gccgccttga gtaaagcctg tctgtttgac ggcggcggcg atgtcggcgg
    12361 tggctttgag attatggata atcacgcggc ggcgcagggt ttgcgcgatg gcaagctggc
    12421 ggctaaaaac ttgaatttgc cgttcacgct gcggcggcgt ttgggttttg tcgtaaaaat
    12481 ccaagccgat ttcgccgacc cacgctcggg ggtaacgcac caatacggct tccaaaccgg
    12541 aaaaatcctg ttccgcaatg ccgtctgaaa accaaggatg gatgcccagc gcaatacgga
    12601 tttgaccgtg ttcggacggc atttccgaca gttccgccac gtcctgccaa tcctgcggac
    12661 aggtggcggg aacgataaac cgccacacgc ccgcctgtcg cgcttcggca agcacctgcg
    12721 ccagattgcc gcgcaaaacg gaatcggcaa gatggcaatg tgtgtcggtc agcctcatct
    12781 tcacaccagc cccaaccgct gcaactcgtg tttcaccccg tccgcgcccc atccttcgga
    12841 aacggcaagc gtcatcaatg cggttgcggt ttccaaattg catttgccgc cgttgaccac
    12901 gcccgcccga cgcagcgcgt cgccctgtgc gtaaacggcg gcagcgcaac cctgcggcac
    12961 ttggctgatg ttaagcaaca atttgccttg ctgcgtaaag tcttgcacgg cgcggatgaa
    13021 gccttcgccg gcggaggtat tgccgtgtcc gtagctttgc aggataaggg cttgggcggg
    13081 aagctgtccg agcccgtcgg caagttcttg gacggcaaag ccggggataa gcgtgcggac
    13141 ggcgattttt gcctgcgggt cgggataacg gatttcgagg ccgtctgaaa catctgccgc
    13201 gtcctgattc gggatgcgga cattgtgcca gccccgggtt tcgtcccact cggcaagtgc
    13261 gccgaaatgg gcattgccga aaccggcggc ggtttccgtg ctgattttgc tgctgccgac
    13321 ggcgggatac agtttgccgt caaaggcaat aagcgtctgc ttcaatccga gtgggaatgc
    13381 ggcaacggca gtcaccagat tgcgcggcgc gtcgctgccc tctgcgttgt acggccattg
    13441 cgaaccggtc aggacgatcg gtttgtccaa accttgcagg gcgagggcga acatattggc
    13501 ggtataggcg agtgtgtccg tgccgtgcag caccaaaaca ccgtcgtact gcggcagcgc
    13561 ggcggcaatg atgtccagcc aagcgcacca gttttgcagt tggacggcgg aagaatcaat
    13621 cagcggctca caaacgtgcc aatcgaaatc catgccgtct gaaaacggcg cgagtgcctg
    13681 cccgaccagc gcggtatcgg ggcgcaagcc cgcactgctt tgcgtcatgc cgatggtgcc
    13741 gccggtataa aggacaaaaa ttttctgttt catggctcat tctgaaaatg caaagaccgc
    13801 ctgaaacgcg aaatgcgtcc ggacggtcga aaatccgatt tactgctgct tctgtgccgc
    13861 ttttttcgct gccttttccg ccttgcgttt ggcgcgtttt tcgctcaatt gtgcgcggta
    13921 aagctgataa tgtcggcgtt tgcgccacca gaaccacgcc agcgccgccg ccagcacgcc
    13981 caatgcgatg aagatgcccg attgcaggct gtgcattttc gccatcagcc aatcgatgtt
    14041 gtgcgcgccg tactcgccca agtaaatcca aacgggcacg gaaatcagtg cggccagccc
    14101 gtccataatc agaaagcgca gatacgatac tttgcggctg atgccggcgg taacgaaaac
    14161 ggcagtccgc aaacccggca ggaaacgggc gacaaacaga acccagttgc cgtatttgtc
    14221 gaatttttcc tgaacctgcg cgtaacgttt cggcgtcatg atgcgcgcaa tcggtttgaa
    14281 cttgaggatt ttctgccccc agatgcgtcc ggcggcaaac atcacgccgt cgcccgccaa
    14341 cacgccgagc ataccgaccg caaacataat atgcggattg gtataaccca tacccgaaat
    14401 cacgccgccc gttaccaagg tcaaatcttc gggaatcggc acgccgaaac cgcagatgac
    14461 caaaacgaaa aacacggccg catagccgta ttcgacaaaa aaggcttcca aaagggcaaa
    14521 catagcggat attccattgt cggagataaa aagtcagaac aagccgaaac attttctgcg
    14581 cgaaacggca ttctatcaaa gattatgccg tctgaaagcg gcaggggggg gcagattcgc
    14641 gccccgccgg acgttcagac ggcatttaaa caggaaaacc gccgctgttg aaacggtttt
    14701 gcctgatttt gcctaaatgc cgccgatggc ggcggcgata cgctccgccc cgtcgcgcgc
    14761 ccaatcggcc tgacgcgcct cgaccatcac gcgcacgacg ggttcggttc ccgaagcgcg
    14821 caacacgaca cgccctttgc cttcgagttc tttttccact tcggccaaca cgtctttcga
    14881 agcctcctgc catttctgac ccttttggat gcgcacgtta atcatcgttt gcggatacgg
    14941 ctgccaatcg gcgcaaacgg cggcgaggtc ttggttcagg atttgcagcg ccgccaagac
    15001 ttgcagcgcg gaaataatgc cgtcgccggt gttatgtttg tccatgcaca aaatatggcc
    15061 gctggcttcg ccgccgatga gccagccgcg ttggttcagc tgttccaaca catagcggtc
    15121 gccgactttg gcgcggcaga aatcgacgcc ctgctctttc agggcgattt ccatcgccat
    15181 attggtcatg accgttccga ccacgccgcc gatgttgata ccttctcggg cgcgggcttt
    15241 ggcgatgacg taaatcaggc tgtcgccgtc gtaaacctgc ctgtttttat cgaccatcat
    15301 caggcggtcg ccatcgccgt ccaaggcaat accgtaatcg gcttcgtttt gcaacacggc
    15361 ggcttggagg gttttggtat gggtcgcgcc acatttttcg ttgatgttgt agccgttggg
    15421 ttcgttgccg atgctgacga cctgtgcgcc gagttcgtgg aacactttgg gtgcaacgcc
    15481 gtatcctgca ccgttggcgg tatcgataac caacttcaaa ccccgaaggt cggaatggcc
    15541 ggggaaggtg gatttgcaaa actcgatata gcggtcgtcc gcaccgctga tgcggcgggc
    15601 gcgtccgaga cgtgcggacg gttgggtttt catttcttca tcgattttgg cttcgatttc
    15661 caactcgact tcatcggaaa gtttcacgcc gccttcggca aagaatttga tgccgttgtc
    15721 ggaataggtg ttgtgcgacg cggaaatcat cacgccggcg gacaggcgca atgcgcgggt
    15781 cagataagcc acaccgggcg tgggcaacgg cccggtctgt accacattca cacccgccgc
    15841 cgtaaagccc gctaccagcg cggcttccag catatagcct gaaatgcgcg tgtctttgcc
    15901 gatgaggacg gtcggtttct gatcggtatc gtgctgcacc agcacctgcc ccgccgcata
    15961 gccgagtttc aatacaaaat cgggcgtaat cggaaactga ccgacttcgc cgcgcacgcc
    16021 gtccgtgccg aaatattttt ttgccatcat ttgctccgtg aatgtgaacc gttgtccgag
    16081 attatacagt cagtttgcgc cctgctgcct gcaccgttga tgccgtctga agccgtcccg
    16141 cccttttcag acggcattcg gcgcaaaaac cgccgtttac cggttcacgc ccaacgcttc
    16201 ccacaccttc aacgcgtccg ccgtcgcctt cacatcgtgc acgcgcacga tttgcgctcc
    16261 gcgtgccacg gaagccaaag ccgctgccac gctgccgtgt acgcgcgccg ccgcgtccgc
    16321 ctcaccggtc agctcaccta tcatgctttt gcgcgacaca ccgatcagca gcggcaaacc
    16381 ggtttccgcc atcaattcgg gcaaatgccg catcagtgcg atattgtgtt gcaggttctt
    16441 gccgaagccg aaacccgggt cgagcgtaat ccgttgcggc gcgatgcctg ccgcgacaca
    16501 ggtttctgac cgtgttttca gataacgtgc cacttcgccg accacatcct gatatttcgg
    16561 attgtcctgc atggtttcgg gcaagccccg catatgcatc aggcaaatgc ccgtgtccgc
    16621 ctggcgcgcc agcaattcga ccgcaccttc gtcggtcaac gccgccacat cattgataat
    16681 atcgatgccg ccgagtgcca acgccttttc cataaccacc gtgcggcgcg tgtccaaact
    16741 gacgggaaca ccccaccccg ccgcttccgc caatacaggc tcaacccgcg cccattcttc
    16801 ttcgggcgga acgaaatccg cacccggccg cgtcgattcg ccgccgatgt cgagaatgtc
    16861 cgcaccctct ttcaaaagcc gttcggcatg cgccaaagct gtttgggcgt tttgcgaata
    16921 cgcgccgccg tcggaaaaag aatcgggcgt gagattcacg atgcccatga ttttcggttt
    16981 gtccaaaccg atttcaaacc gtcctgcccg ccaaacgtgt cgtgccatct gaactcctcc
    17041 cgaaataaaa aacagattat atgccgtctg aaaccgtctt gtgcgcttcg gacggcaccg
    17101 ctattcgggc ggcagacggc atgttgtccc aatgtctgcg ccgcctttga atctgccggc
    17161 atgcctgcta tccgcccgcc ttttcaaaac aggttccgac gattccgcac gcgcctgccg
    17221 cctttgccaa gccgtacagg atttcctgcg gcatatcgcg gctccataat cccgtaatat
    17281 tcgcaatcac gggcagatgg ctgatttggc ggactttcac gatggattcg acatccaaac
    17341 ggtagggatg gcctttggta tggcccaaca cgcccgactc gcccagaatc aggtggcggt
    17401 tgccgcgcga gacgacatat tctgcggcat tcaaccaatc ttcggcagaa tggtgcttgt
    17461 ctttacacag aaccaaggga atgttcaggc ttccggcttc attcaatacg gcaagatcgg
    17521 acatcagccc gccgcccaaa tacaggatgt ccgctcccgc attcaaagcc gcttcgacat
    17581 ggcggacgtt gcgaacgcgc accaatacgg gtttccctgc gccatgcgcc gatgcggcct
    17641 gttccgccaa ccgtctgcac cgtccccgcc cttcatccgc acttaaagtg tcgtataagt
    17701 ttgccgaagt gaaaaacgga tccagaaaca ctgcatccgc attgctccat actgacggtt
    17761 ctgcagcgat acggacggtt tccccgccgc cgaaagccac gcctttggcg gcaacgcggc
    17821 tgtcttccgc ccgattttcc cgactgacgg ttttccatgt gtccaaaatg cggacggctt
    17881 tctcgacctc cggcagcgtc tgtacctccc tgacgctcaa aaccctatcg tcgccgattg
    17941 cgccgatgac ggtacgctcg tcgccgtgag aaatgtgttc tcgcagacct ctgctgcgga
    18001 tgaaggcgac aacgccggca atgtccgctt cggcggcacg cctgctcatg acaataatca
    18061 tatttcctcc tgacacaaga gacggcccgc ccaaaatagg atttttgcaa gccgtgttat
    18121 actgtggcgt gttttacaga ttgttcgggc tatggattta ttatcggttt tccacaaata
    18181 ccgtctgaaa tatgcggtgg cggtgctgac gatgctgctt ttggcggcaa tcgggctgca
    18241 cgcttccgta tatcgcacct tcacgcccga aaacatccgc agccgcctcc aacaaagcat
    18301 tgcccatact caccggaaaa tctcgtttga tgcggatata cggcgcaggc ttctgccccg
    18361 cccgaccgtc atcctgaaaa acctgaccat taccgaaccc gacggcggcc gggtcgccgt
    18421 ttccgtcaaa gaaaccaaaa tcggattgag ctggaaaaac ctgtggtcgg atcggataca
    18481 ggttgaaaaa tgggtggttt cgggtgcgga tcttgccctg acgcgcgaca gaaacggcgc
    18541 ttggaacatc caagacctgt tcgacggcgc gaaacactcc gcctcagtca accgcattat
    18601 cgtcgaaaac agcaccgtcc gcctcaattt cctgcagcaa cagcttatcc tgaaggaaat
    18661 cagcctcaac ctgcaatccc ccgattcgtc ggggcagcag tttgaaagtt cgggcatact
    18721 ggtttggaga aagctgtccg tcccgtggaa aagcaggggg ctgttccttt cagacggcat
    18781 cggcacgccc gaaatctcac cgttccattt tgaagcttcc acttcgctgg acggacacgg
    18841 cattaccatt tccaccaccg gcagcccttc tgtccgcttc aacgccggcg gagcggatgc
    18901 cgccggcctc ggcctgcgtg cagacacttc cttccgcaac ctccacctga ccgcgcaaat
    18961 ccccgcattg gcactcaaaa acaacagcat caaaaccggc acggtcaacg gcacgtttac
    19021 cgccggcggc gaatatgccc gatgggacgg ttcgttcaaa ctcgacaaag ccaacctgca
    19081 ctccggcatc gccaacatcg gcaacgccga aatctccggc agcttcaaaa caccgcgcct
    19141 tcaaaccaat ttctccctcg gctcgccgtt ggtttggagt cgggacaacg ggctggacgc
    19201 cccgcgcctg cacatatcga cccttcagga taccgtcgac cgcctgccgc aaccccgttt
    19261 catcagccgg ctcgacggtt cgctgtccat accgaatctg caaaattgga atgccgaatt
    19321 aaacggcaca ttcgaccgcc aacccgttgc cgcaaaattc aaatatacgc gggaaggcgc
    19381 accgcacctg gaagccgccg ccgcgctgca aaaattaaac ctcgccccct atcttgacga
    19441 atttcggcaa caaaacggca aaatattccc cgacatcctc ggcaggctgt ccggcaacgt
    19501 cgaggcacac ctcaaaatcg gcagcatcca actccccggc ttgcaactgg acgatatgga
    19561 aacctacctc cacgccgaca aagaccatat cgcgctcagc cgtttcaagt cagggcttta
    19621 cggcggccat accgaaggcg gcatcagcat cgccaacacc cgtcccgcca cttaccgcct
    19681 gcaacagaat gcaagcaaca tccaaatcca accgctgctg caagacctgt tcggcttcca
    19741 cagcttcagc ggcaacggcg atgcggtcat cgacctgacc gcaagcggcg aaaaccgcaa
    19801 acagcttatc cgctcgctgc aaggcagcct gtcgctgaat atttccaacg gcgcgtggca
    19861 cggcatcgat atggacagca ttttaaaaaa cggcctttcc ggaaaaatct cgggcagcac
    19921 acccttctac cgattcacgc tcaacagcga aatttcagac ggcatcagcc gccacatcga
    19981 taccgaactc ttctccgaca gcctctatgt taccagcaac ggctatacca atctggatac
    20041 gcaggaattg tctgaagatg tccttatccg caacgccgtc catccgaaaa acaaaccgat
    20101 tcccctgaaa atcaccggta cggtggacaa gccgtccatt accgtcgatt acggcaggct
    20161 gaccggcggc atcaattcgc gcaaagagaa acagaaaatc ctcgaagaca ccctgctgga
    20221 acaatggcag tggctcaaac ctaaagaacc gtaaataccc tgcgtacaaa aatgccgtct
    20281 gaaacacatt aatcttcaga cggcatgacc gtaaaaccta caaccccaat ttcccccaaa
    20341 tcccatcaat tttaaccgta accgcagggt cttttttgat tacccgtccc cattcgcggt
    20401 cggtttctcc cggccatttg ctggtcgcat ccaaacccat tttgccgccg agtccgctga
    20461 cggggctggc gaagtcgagg tagtcgatgg gcgtgttttc caccaaaacg gtgtcgcgga
    20521 cggggtccat gcgcgtggtt accgcccaga tgacttcttt ccagtcgcgc acgtttacat
    20581 cgtcatccac cacgatgatg aatttggtgt acataaactg gcgcaggaac gaccagcagc
    20641 ccgtcatcac gcgcttggcg tgtccggcgt actgtttttt catgctgacc accgccatgc
    20701 ggtaggaaca gccttcgggc ggcaggtaga aatcggtgat ttcggagaac tgcttttgca
    20761 aaagcggtac gaacacttcg ttcaacgcca cgcccaaaac ggcaggttcg tcgggcggtt
    20821 tgcctgtgta ggtagagtgg taaatcgggt tttcgcgcat ggtgatgcgt tcgaccgtaa
    20881 acacagggaa atggccctgc tcgttgtaat agcccgtgtg gtcgccgtat gggccttcca
    20941 acgcggtttc gtttggatga atcacgcctt ccaatacaat ttcagcacgg gcaggcactt
    21001 gcaaatcgct gccgatacat ttcaccagct ccgtccgcga accgcgcagc agtccggcaa
    21061 actggtattc gctcaaagta tcgggaacgg gcgttaccgc gcccaaaatg gtggaggggt
    21121 cgcaaccgag tacgacggcg acgggatacg gcgtatcggg attgagtttg cggaattcct
    21181 gataatccaa cgcgccgccg cgatgcggca gccagcgcat aaccagcttg tttttgccga
    21241 tgagttgttg gcggtaaatg ccgagatttt ggcgtttttt gtgcggcccg cgcgtgacgg
    21301 tcaagcccca cgttaccagc ggcgcaacgt cttccggcca gcaatgttga atcggaagct
    21361 gatacaaatc aacgtctcct ccttcccaca cgatttcctg acacggcgcg tttttcacca
    21421 cgttcggcgc catgctccaa atatctttca acagcggcag tttggaaaac gcgtctttga
    21481 tgcctttggg cggttcgggt tctttcaaat acgccagcgt ctgcccgatt tcgcgcagct
    21541 tggacacgct gtccgcgccc atgcccatcg ccacacgttc gggggtgccg aacaggttcg
    21601 ccaacacggg ataatcatag cgcgtaccgt cgggcttaac cgggttttca aacaacaacg
    21661 ccggcccttc ggcgcgcaac acgcggtcgg caatttcggt catttccaaa tgcggggaaa
    21721 cggggtgggc gacgcgcttg agcttgccct gctgctcgag catagcgatg aagtcgcgca
    21781 ggtctttgta tttcatgttt atcctttttg tccttttatc ctgaacaatc cgattcggat
    21841 accgccccta tccttgcctg cgcttcggca tatcccatgc cgtgataaaa gtcgcgtacc
    21901 ggcggatgtc cgccgctttg atgaagttgc agcaaagggc gttggttgtc ggactgggta
    21961 acgacattgc agtgcagacc gaaggtgtcg gtttcataag ggggcagccg attgcaaatc
    22021 ataccgaaat aaacggcgtt ttcagggttg tcgtaaaagc ggctttgata gtcgttaaaa
    22081 ctcttttcgc tgacggacac ccacacgccg tattccagcg tttcctgatg cccgataatc
    22141 ggaataggca gcgccgcgcg gataaagcgg tcggtttggt cggaatagcg gatgatgcag
    22201 aaatcagaat cgcattccgc ttgataagca atgcgttctt cttcactgag ttgattatag
    22261 ggataggggg cggtaaagcc ggttgcgggc atatcttcat gtttttcgcc gcaggaagtg
    22321 caagtgtgca taaggtttca gactttcaac acaagtttgc ggtaaagcca ttcgccggca
    22381 aacagcatcc ccatcaatac ataggcaatc acgcctgtat aaaccgccca ccaatcatat
    22441 cgccccaacc gggccaacaa agcggcaagc gtcccgttgg caataaagaa tacgcaccaa
    22501 acctgcgtta cccggcgggt atagcgcacg gctttttcag gcaggtcggg ctgttgcagc
    22561 cgcgcaagtt tttcaatcac cgtctgcccg gcaaacaggc tgctgccgaa caccgccaac
    22621 atcatcaaat taacgaggac aggataccaa tacattgaat cacgccgccc gaacaccaat
    22681 actgcggcaa aaaacagtgt aatatagtgg attaaattta aatcaggaca aggcggcgaa
    22741 gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ccgtaccggt ttaaatttaa
    22801 tccactatac cacaaagcgg gataggcaat gcttaatacg gtcaaaaaaa tatgtccgaa
    22861 aaaaccgggt ttcattttga atccgcacaa acgttttcag acggcatccg ataaaaacat
    22921 gccgtctgaa aaataattag aaatacccga ttagccggcc tgaatcttca ataccgcctg
    22981 taccacgtcg ttgacggtgc ggacattgcg gaaatcttca gcctgcagct tgcgggctgc
    23041 cacaaccatt cacgattgcg atatacccgg cggcattccc gactgccgtt cagcagaaaa
    23101 cgcacccatt ccaaaccgct ccaatatgcc tcgggcagca taccggcttc agacggcata
    23161 tcgtccgaag cagacaaagt caggctgtaa cgcgtccctt tggtcagaac catcgccaaa
    23221 gcataagcaa acggcgcgcg cgttgccgat acggcatatc cttccggcag cggatcgtcc
    23281 gccgccaaaa ccaaaaccga cccgcatccc tcttccaaca gggacgccgc ttccgccaat
    23341 gccgtttcca caccgtcggc acacacggcc aatgccgtct gctcgctcat atcccgcctc
    23401 aatatcgacc attgccccgc cgtcgcattg tgcaccgaca aaccgaacga agtcggcgac
    23461 acggtatgcg atttcaacag ttccaaccac aaatcgaaac tacgcgccat ttccccgtcg
    23521 tgcgaggcat aaactaccgg actgccggga tgggcggagg caatgtccca agccgcgtcg
    23581 cataccaaac gcgccgcctt actcaaacgg cggcgccgca tagcgggcag gaacggcaat
    23641 tcccgcctga catcgggcaa accgtcggca aaatccggac attccgccca ctgcctccac
    23701 tgggccatat cgcgcatttt gccgcccgaa acccgccagt cggcgatgtc gaagtggaac
    23761 cggcaaataa tcggcggcat agtttctttc aaaaatttac actgtgccgc attctaacca
    23821 aagcccatcc ccctgacaat gccgaaattc aaacgcatcc acgccatttt ttccgacaac
    23881 gccgcccctc ggcaaaccgc ccgaattagc ctgaatttac atttatcatt ataatgcccg
    23941 attaccgaca ccgcctccct gctgccgcac agcgggcgta tggttctgat agaccgcatt
    24001 acccgatacg gcgatgattt tgtcgaagca ggggcgcagg taaaacccga ccacatcctg
    24061 ctggccggcg gcatactgtc ctacacggca tttatcgaac tgatggcgca ggctgtcggc
    24121 gcatatgccg gcctccaagc ccgaaaaaag gcgcaaccgg tccggctcgg ttttcctgcc
    24181 cggcacgcgc aaacttgaaa tttttaccca atccgtccct gtcggaacac atttgccggc
    24241 aacggcgcat atgtctatcc aagatgccgg ggtatgggcg tgtccgactg cgaactgcgt
    24301 tggacggatg cgccggaaac ttcgtccgga acactccctt cagacggcat tttggcgcgc
    24361 gcctcactca acgtgtacag ccccgaacac cctgtcgaaa caacttaatg ccgtctgaac
    24421 agacacgcac atacggagaa caaccatgac tgaaaccgtc ctgattaccg gctcaaacag
    24481 aggcataggc aaagccgccg cactcggttt tggcggacga cggctttgat atcgccgtcc
    24541 actgccgcag ccgccgcgac gaagccgaag ccgtggcgga agaaatccgc gctttgggca
    24601 gaaatgcgcg cgtgttgcag tttgacgtgt ccgaccgcga agcctgccgc gagattctga
    24661 ccgccgacat cgaagcaaac ggcgcgtatt acggcgtggt gttgaacgcc ggcctgacgc
    24721 gcgacaatgc cttccccgcg ttttcagacg acgattagga tgtggtgccg cggactaatt
    24781 tggacggttt ttacaatgta ttgcatccgc tggttatgcc gatgatacgc cgccgcaaag
    24841 ccggacggat tgtgtgtatg gcatcggtgt ccggcctgac gggcaaccgg gggcaggtca
    24901 attacagcgc gtcaaaagcg ggcattatcg gcgcggcaaa agccttggcg gtcgaaccgg
    24961 cgaaacgcaa aatcaccgtc aactgcgtgg cgccgggttt gatcgatacg gagattgtcg
    25021 atgaaaacgt acccgtcgga gaaattttga aagccgtccc cgccgcgcgt atgggactgc
    25081 cggaagaagt ggcgcacgca gtgcacttcc tgatggatga aaaagcggcg tatatcacgc
    25141 gccaggtgat tgcggtgaac ggaggtttgt gttgaatacc agaagggtcg cagtaacggg
    25201 cataggcggc attaccgcct tcggccggga ttggcaaagc atacaggcag cattcaaagc
    25261 cgaaaaaaac gccgtcaaat atatggattg gcgcgaacgt ttccccgaat tggaagcgca
    25321 actgggtgcg ccgattgagg attacgcgcc gccgaaacat tggacgcgca agcggctcag
    25381 aagtatgggg cgcgtgtcgt acctgtgcgt cgatgcggcg gagcaggctt tgatggatgc
    25441 aggtttgctc ggggacgaaa gcattaccga cggacggatg ggcgttgcct gcggctcttc
    25501 cggcggcggc accaaagaca tcggcgatgt gggcgaattg ttgctgaccg gcacgtcgcg
    25561 caacttcggc gccaatacct atgtgcgtat gatgccgcac accgccgacg ccaatatcgg
    25621 catctttttc ggattaataa ggacgcatca tcccgatatc gagtgcgtgt tcttcgggca
    25681 gccaaggcat aggttatgct tacgaggcca tcaaatacgg tctgaccgat ataatgctgg
    25741 cgggcggcgg cgaagaattt tgcccgtccg aagtgtatgt tttcgactcg ctctatgccg
    25801 ccagccgccg caacggcgaa ccggaagaga ccccgcgtcc atacgacgcg aaccgcgacg
    25861 ggctggtcat cggagaaaag gcgcggggat tttcgtattg gaagaattgg aacacgccaa
    25921 acggcgcggt gcgaaaattt acgccgaact cgtcggctac ggcgccaaca gcgacggcat
    25981 caatatcggc gaaggcgcag ccgtattcat catgacgcgc gatgcggatt tttccggcgg
    26041 tatgcagctt ctgggttacg gcgcaagcag cgatgcctac catatttcca cgccccgccc
    26101 cgacgcaaag gcgcaatcct cgcctttcag acggcattgc agcacgccgg ccttgcgccc
    26161 gaagacatcg gccggattaa tctgcacggc accgggacgc accacaacga cagtatggaa
    26221 agccgcgccg ttgcagcggt tttcggcaac aatacgccct gcacgtccgc caagccgcaa
    26281 accggacaca cgctgggcgc ggcgggcgca atcgaagccg cgttcgcgtg gggcattgcc
    26341 gaccggcaaa gcaatcccga agggaaactt ccgccccggc tttgggacgg gcagaacgac
    26401 cccgacctgc ccgccattaa cctgaccggc gacggctggg aaaccgaaaa acgcattgcc
    26461 gccagctcgt cgtttgcctt cgggggaagc aactgcgtct taatcatcgg atgaaataag
    26521 tttgtcaatc ccaccgctat gctatacaat acgcgcctac tcttgacggg tctgtagctc
    26581 aggggttaga gcaggggact cataatccct tggtcgtggg ttcgaacccc accggaccca
    26641 ccaattccca agcccggacg tatgtttggg cttttttgcc gccctgtgaa accaaaatgc
    26701 tttgagaaac cttgataatg aaaaaagtca gcgtattgat tgttgccaaa aacgaagcaa
    26761 accacattcg ggaatgtatt gaaagttgcc gtttcgataa agaagttatc gttatcgacg
    26821 accatagcgc cgacaacact gccgaaattg ccgagggttt gggtgcaaaa gtcttcagac
    26881 ggcatttgaa cggggatttc ggagcgcaaa aaacatttgc catcgaacag gcaggcggag
    26941 aatgggtttt cctgattgat gcagacgaac gctgcacgcc ggaactatct gatgaaatct
    27001 caaaaattgt ccgaaccggc gattatgccg cctattttgt cgaacgccgc aaccttttcc
    27061 ccaaccatcc cgccacacac ggcgcgatgc gtcccgacag cgtatgccgt ctgatgccga
    27121 aaaaaggcgg ttcggtgcaa ggcaaagtac acgaaaccgt acagaccccc taccccgaac
    27181 gccgtctgaa acattttatg taccattaca cgtacgacaa ctgggaacaa tatttcaaca
    27241 agttcaacaa atatacttcc atctcggccg agaaataccg ggagcaggga aagcccgtga
    27301 gtttcgttag ggacattatc ctccgtccga tttggggttt tttcaaaatt tatatcctga
    27361 acaaagggtt tcttgatgga aaaatgggtt ggattatgtc cgtcaaccac agctattaca
    27421 cgatgattaa atatgtcaaa ctatattatc tgtacaaatc cggcggaaaa ttttaaatgg
    27481 aaaaagaatt caggatatta aatatcgtat cggccaagat ttggggtggg ggcgaacaat
    27541 atgtctatga tgtttcaaaa gcattgggac ttcggggctg caccatgttt accgccgtca
    27601 ataaaaatga tgaattgatg cgcagacgct tttccgaagt ttcttccgtt ttcacaacgc
    27661 gccttcacac gttcaacggg ctgttttcgc tctacgcgct tacccgcttt atccgggaaa
    27721 accacatttc ccacctgatg atacacaccg gcaaaattgc cgccttatcc gtacttttga
    27781 aaaaactgac cggggtgcgc ctgatatttg tcaaacataa tgtcgtcgcc aacaaaaccg
    27841 atttttatca ccgcctgata cagaaaaaca ccgaccgctt tatttgcgtt tcccgtctgg
    27901 tttacgatgt gcaaaccgcc gacaatccct ttaaagaaaa ataccggatt gttcataacg
    27961 gtatcgatac cggccggttc cccccgtctc aagaaaaacc cgacagccgt ttttttaccg
    28021 tcgcctacgc cggcaggatc agtccggaaa aaggattgga aaacctgatt gaagcctgtg
    28081 tgatactgca tcggaaatat cctcaaatca ggctcaaatt ggcagggcac ggacatccgg
    28141 attatatgtg ccgcctgaag cggggcgtat ctgcttcggg agcagaacca tttgtttctt
    28201 ttgaagggtt taccgaaaaa cttgcttcgt tttaccgcca aagcgatgtc gtggttttgc
    28261 ccagcctcgt cccggaggca ttcggtttgt cattatgcga ggcgatgtac tgccgaacgg
    28321 cggtgatttc caatactttg ggtgcgcaaa aggaaattat cgaacatcat caatcgggga
    28381 ttctgctgga caggcttacg cctgaatctt tggcggacga aatcgaacgc ctcgtcttaa
    28441 accccgaagc aaaaaacgca ctggcaacgg caggacatca atgcgtcgcc gcccgtttca
    28501 ccatcaacca taccgccgac aaattattgg atgcaatata agccgctttc agacggccta
    28561 tgccgtctga aagcctttga tgcaacaaac cgccaaatta tattcgttca ttggaaaaaa
    28621 acaccccgaa ttcatccttc aaaataagaa aatcccaata tcccccgata tcgcgcagcc
    28681 tattggcaaa gttttgcagc gtcttccccg gcttgtgccg ccgcgtcaag tgctttgtta
    28741 caatgcacta ccttcacatt tcttaataaa ttttatgagt aaccatactt cttggtcgtc
    28801 caaaatcggt ttcgtacttg ctgcggcagg ttcggccatc ggtttgggcg cgatttggaa
    28861 atttccttat acggcgggca ccaacggcgg cgcggtgttt ttcctgctgt ttttgatatt
    28921 taccgtcttg gtcgccctgc ccgtccagct tgccgaattt tatatcgggc gcacgggcgg
    28981 taaaaatgcc gtcgattcct tcagggttct gcgtccgggt acgcaatggc tttgggtcgg
    29041 gcgcatgggc gttgccgcct gctttatttt gctgtcgttt tacagcgtgg tcggcggatg
    29101 ggtattgaat tatgtcgtcc acagttttac aggggcaatc catgcgggag cggactttga
    29161 agccctgttc ggcacaacga tttccaatcc ggcaggttcg ctgtcctatc aggcactgtt
    29221 tatgctgatt acggtttggg tggtcaaagg cggcatttca gacggcattg aaaaggcaaa
    29281 ccgttatctg atgccggggc tgtttatcct ctttattgcg ctggctgtcc gttcgctgac
    29341 gctgccggat gcaatggagg gcgtgtcttt cctgctcaaa ccgaattggt cgtactttaa
    29401 agccgatacg atgattacgg ctttgggcca ggcgtttttt gccctgagca tcggcgtttc
    29461 cgccatgatt acctacgctt cgtatttggg aaaagatcag gatatgttcc gttccggcca
    29521 tacgattatg tggatgaacc tcttggtttc cctgcttgcc ggcctggtga ttttcccggc
    29581 ggtattcgcc ttcggttttg aaccgagcca ggggccggga ttgattttta tcgtattgcc
    29641 cgcagtgttc atgaaaatgc cgttcggtac tgttttattt gcagtattca tgcttctggt
    29701 cgttttcgcc acgctgactt cggcattttc gatgttggaa acggtcattg cctcaaccat
    29761 ccgccaagac gagcgcaaac gcaaaaaaca tacttggctt atcggcacgg ctattttcat
    29821 tgtcggcatc ccgtccgcgc tgtctttcgg cgcatggggt gagtttaagg ttttcggcaa
    29881 aaccattttt gatttgtggg actatgttat ttccgccgtc attatgccga ttggtgcttt
    29941 gagtgtttcc atctttaccg cctggattca ggacaagcag tctgtgttaa aagatgccgg
    30001 cgcgggcagc accgtaccac gggcagtgct gctgctgtgg ctgaatacct tgcgctacct
    30061 tgccccgatt gccattatta ttgttttcgt caatttttta ggcatccttt aaacataccg
    30121 cccgacggca aaatgccgtc tgaaagcctt tcagacggca ttttgcttca gattcaacct
    30181 atttcgttca aagtatagtg gattaaattt aaatcaggac aaggcgacga agtcgcagac
    30241 agtacagata gtacggcaag gcgaggcaac gccgtaccgg tttttgttaa tccactatat
    30301 tgtccggcat ggccagccta actttaagcc gcctgctttc ggcaaccgac cgtatcagcg
    30361 gcaaatccat cacggaaaac gccgtcgaca aggcatcggc aaccgccgca tcatccgcca
    30421 ttacgctcat gcttttataa cgcggcgtac tcacgccggt tcgcggatcg aacaaatggg
    30481 taagcctgcc cgctttgtcc atcaccgtcc catagccgcc cgaagtggca aacgccttgt
    30541 ctttcaccgt gatatttgcc aacacgcctt cttcgtcgtc cggattgcgg atgccgacat
    30601 tccacatccg cctgccgttt gtatcaaacc ccctgatttc gcccatatcg accagcgcgg
    30661 caggcactcc gtttgcttta agcagcgcaa caaccttgtc cgtgatatag ccttgcgcga
    30721 tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg tcgtcaaacg
    30781 agactttgcc gaagccgacc cgtttcaggg tttccttcac gctattctcc ggcggcgttt
    30841 ccgcatcggg acgggcggca aaataatcgg catacagttt ccacaacgcc tgaaccgtcg
    30901 gatcgaacgc gccttccgtc agcgcgtgaa tatcgcggcc aggctcaaca gttccaaaaa
    30961 atccgccgga ggcgaagtca gataaccgtc gcggttcaga cggctgatca ggctgtcttc
    31021 acggtaaagg ctgaacattt tttccaaacg cgccacttcc gccaaaacct tattgaccaa
    31081 atccgccgcc tgtctgtcgt ccacgccgaa caggcgcagc tccgcgccgg aacccagcgc
    31141 gacacctttc cagaaaaaca cattttcatc gcgtttttta tccatgttgc gtttttcgcc
    31201 gccggcggca aaaggattcg gcaaaaaaga aaccgccgca ccggccacag caacggcggc
    31261 agccgtcaga aaacgtctgc gcccgaaatg cctgcccata ccgcctccta aaccgacact
    31321 accgccttga tgtgcggatg cggatcgtag ccttccaact cgaaatcttc aaacttgaag
    31381 gcaaacaaat ccttgacttc aggattgatt ttcatcacag gcaaggcgcg cggttcgcgt
    31441 tccaactgca atgcggcctg ttcgaaatgg ttacggtaca aatgcgcgtc gccaaacgta
    31501 tggacaaact cgcccgcctc cagcccgcac acttgcgcca tcatcatcgt caacagcgcg
    31561 tagctggcaa tattaaacgg cacaccaagg aaaatatctg cactacgctg gtaaagctgg
    31621 caggacagtt tgccgtcggc aacgtaaaac tgaaacagcg cgtggcaggg cggcaaggcc
    31681 atctcatcga ccaaagccgg attccacgcc gacacaatca ggcggcgcga gtcggggttt
    31741 ttcttaattt gttccaccac attggcgatt tggtcgatat gcctgccgtc gggcgcgggc
    31801 cagctgcgcc attggtagcc gtaaaccggg cccaagtcgc cgttttcgtc cgcccactcg
    31861 tcccaaatgg aaacattgtt atccttcagg tatttgatat tggtatcgcc tttgaggaac
    31921 caaagcagct cgtggattat cgagcgcaaa tgcagctttt tggtcgtcag cagcggaaaa
    31981 cctttgccca agtcaaaacg catctgataa ccgaatacgg agcgcgtacc cgtaccggtg
    32041 cggtctgatt tgtccgtacc gttgtcgagg acgtggcgca tcaagtccaa ataggccttc
    32101 atcatcatct ttcatcaaat taaacggcac atattgtaac atttccggcc aacacccgag
    32161 tccggacaaa cgcgcaggcc gccccgccgg acgctcaaaa cgcggatgaa ggcaggcagg
    32221 attgttggca atttcagccc tttttcacag caaaagcagg cgtgaaaaca aaattatctt
    32281 gattggaatc aaaaaatcta gtttaactac ttagaataaa attccaataa tattttttat
    32341 ttgagaaatt aatttatgat tattggttga ttatcggcaa taaacatcat atatcgaaaa
    32401 tatgggaaaa ataatgtcaa caatttgtgc aaaattaggc ttggcatcag aaaaaaatag
    32461 gtttatattg ccaactacaa atttgttttc ccattagtac aatatcaacc aaaaggagta
    32521 tccgaatgac tgacctgaac accctgtttg ccaacctcaa acaacgcaac cccaatcagg
    32581 agccgttcca tcaggcggtt gaagaagtct tcatgagcct tgatccgttt ttggcaaaaa
    32641 atccgaaata cacccagcaa agcctgctgg aacgcatcgt cgaacccgaa cgcgtcgtga
    32701 tgttccgcgt aacctggcag gacgacaaag gacaagtcca agtcaaccgc ggctaccgcg
    32761 tgcaaatgag ttccgccatc ggcccttaca aaggcggcct gcgcttccac ccgaccgtcg
    32821 atttgggcgt gttgaaattc ctcgcttttg aacaagtgtt caaaaacgcc ttgaccaccc
    32881 tgcctatggg cggcggcaaa ggcggttccg acttcgaccc caaaggcaaa tccgatgccg
    32941 aagtaatgcg cttctgccaa gcctttatga ccgaactcta ccgccacatc ggcgcggaca
    33001 ccgatgttcc ggccggcgac atcggcgtag gcggacgcga aatcggctac ctgttcggac
    33061 aatacaaaaa aatccgcaat gaattcactt ccgtcctgac cggcaaaggt ttggaatggg
    33121 gtggcagcct catccgcccc gaggcgaccg gctacggctg cgtctatttc gcccaagcga
    33181 tgctgcaaac ccgcaacgat agttttgaag gcaaacgcgt tctgatttcc ggttccggca
    33241 acgtggcgca atacgccgct gaaaaagcca tccaactggg tgcgaaagtg ctgaccgtct
    33301 ccgactccga cggcttcgtc ctcttccccg acatcggtat gaccgaagcg caactcgccg
    33361 ccttgatcga attgaaagaa gtccgccgcg aacgcgttgc cacctacgcc aaagagcaag
    33421 gtttgcaata ctttgaaaac caaaaaccgt ggggcgttgc cgccgaaatc gccctgccct
    33481 gcgcgaccca gaacgaattg gacgaagaag ccgccaaaac cctgttggca aacggctgct
    33541 acgtcgttgc cgaaggcgcg aatatgccgt cgactttggg cgcggtcgag caatttatca
    33601 aagccggcat cctctacgct ccgggcaaag cctccaacgc cggcggcgtg gcaacttcgg
    33661 gcttggaaat gagccaaaac gccatccgcc tgtcttgggc gcgcgaagaa gtggacagcc
    33721 gactgttcgg catcatgcaa agcatccacg agtcctgcct gaaatacggc aaagtcggcg
    33781 acaaagtgaa ctacgtcaac ggtgcgaaca tcgccggctt cgtcaaagtt gccgatgcga
    33841 tgctggcgca aggcttctaa gcaaacgcct ccgccccgca aacaaaatgc cgtccgaacc
    33901 gcaaatgctg ttcagacggc atttcattat ccgcccgttc gaatcgggta atactatagt
    33961 gaattaaatt taaatcagga caaggcggcg agccgcagac agtacaaata gtacggcaag
    34021 gcgaggcaac gccgtactgg tttaaattta gttcactata ccgatacatc cgaatatgcc
    34081 agaccgtctg aacggtattc atacctccca atcctgcacg cgcttcaaat cgttttgcgc
    34141 caaagtatcc gcgtggcggt tacggctctg atattccctg tctttcaata tgctgctcgc
    34201 cacataattc aaatgagcct ttgccgcctc cgaagcctcg cccggccggc ggttcgatat
    34261 cgcctcatac agcacgcggt gctgcgccat cagtttcgga cgcggatctt cttcctgatt
    34321 cagataaata aggctgctgc gcgtctgccg gtacagcatt ttcaacaaac cgcccgacaa
    34381 atggctgaac aacaaattgt gcgccgcatc cgcaatcgtc tgatggaagc tgacatcggc
    34441 ttcgccctga tgttccagat tgccgcttcc gcacgcttcc tcaaactttt caagccaaaa
    34501 ccgaatccgc ttcaaatcgg catccgtccg gcgttctgcc gccaatgccg ccatacagcc
    34561 ctcgatgtgg cagctgaaat caaaaacatc ctgttcccaa ttggaatgtt tgcccaaaag
    34621 ctcctgccaa ctttgcaaaa aatcctgctg cggcttgacc gaaacataat aaccatcgcc
    34681 ctgcctcgct tccaaaatct gacgggcaac caaaatattc aatgccgacc ggaccgacgg
    34741 acgcgaaacg ccgaactctt ccgccaaaac gcgttcgggc ggaatcttgc ccccttccgc
    34801 gtaaacccct tccacgatac ggccttccaa taccgacaat acctgatcgc tgattttctg
    34861 aggccttacc agtttcatca ctcctccttt ataaagattc cctgcagaac ccttccgaaa
    34921 tacatcaaac atcaaattgg actggccaat cagggcggat tctaatgaca cgccgttccc
    34981 gccgtcaacg gcacttacct cgcaccgccc ccgaaacaca ggaaaaaact acaccaaact
    35041 acaatttttg ttcatgcaaa tatttgtttt aacgggattt aaatgaaagc tccgattcaa
    35101 atctgccgaa ccgcccaaaa atatattgac ctgaatatta aagtttcgta aagtaatgca
    35161 acgttgcttt aattggtttg accactattg ccgacgatta gaaaaatatt ttcggagatg
    35221 ttcaattatg gaaacttggg ttcaaaacta cacggcaatc ggcggcagcc tgtatctgac
    35281 tgccaccgcc gcactcttac ccatcgtctt tttctttgcc gcgctgaccg tcctgaagct
    35341 gaaaggctat caggcggggc tttatacgct gctgattgcg cttgccgttg ccgtattcgg
    35401 cttcgggatg ccgacgggta tggcggtttc ttccctgtcg ccgcaaccgc attgacccaa
    35461 cgccccatac gctacactgg acttgaccgc gcattacaaa atcggcaaat ccacccgcat
    35521 cggtttggac tttgaaaacg tctttaacaa acgctgccgc acgatgcccg acattcacgt
    35581 ttacggcacg ccgcgcagcc tgaccgcaac cgtcaaacat atagtggatt aacaaaaatc
    35641 aggacaaggc ggcgggccgc agacagtaca aatagtacgg aaccgattcg cccggtgctt
    35701 gggcgcctta gggaatcgtt ctctttgagc taaggcgggg caacgccgta ctggtttttg
    35761 ttaatccact ataaagaaag aaatgccgtc tgaaacctta tcgtttcaga cggcatttgt
    35821 ttgcccgggc ggttatttgc cggtttgggt atcccgtttc aatccgccgc cgagcgcctt
    35881 gtacaaatcg gcaaggtttt cggcgcgggt cagttgtgcc gacaaagccg caccttccgc
    35941 cgaatagctg ctgcgttccg catcgagcaa atcgagcgcg ccggatacgc cgtgtttgta
    36001 acgcagtccg accaagcgca acgcttcttt agaggcgcgg ctttgtttgc ttaaagcgtc
    36061 ataggcttta tccagctgct cgcgcgccgc caatgcgttt gccacgtctt gaaaggcgga
    36121 ttggacggcg gattcatagg caacaatttg tgcctgttgg cgcagttttg ccacatcaag
    36181 gttcgccttg ttcgttcccc aagtaaaaat cggcagggta atagacggcg cgaacgccca
    36241 aacgcccgtg ccgcttttga acagcccgcc caattcgaca gaacccgtac cgacgcttcc
    36301 ggtcaggcgg atggacggga aaaaggcggc gcgcgccgca ccgatattgg cgtttgcctg
    36361 tttgagcgcg tgttcggcgg cgcggatgtc gggacggtcg agcaatactt cggaactcaa
    36421 accggcaggc agtttttcaa caaaaaactg cttgtccaac ggcaaaccgg cgggcaggtc
    36481 ttcgggtatc ggacggttaa tcaaggttgc caaggcattg cgcgcctgtt cgcggctgcg
    36541 cgcggcatgg gcataatcgg ctttggcaga ttcaatcaag gcttcctgct ggcgcagggc
    36601 gacggcggaa atcacgcctg ccttgtaccg caattcggac agcttgtagg tttcctcgcg
    36661 cgttttcaag acacgctgcg ccaaagacat cgcttcttcg gcataacgct cgttgaaata
    36721 ggctttggca acggtggcaa tcagactcaa atgtgccgca tcgcggttgg caacgctggc
    36781 aaaatagcct tgcagtgctg cttcgctgct gctgcgcacg cgcccgaaca gatcgagttc
    36841 gtaagatgcc gcacccagtc cgacattgta gctgctgctg acattgccgc cgctcaagct
    36901 gccttggcgc gagccgttcg cattggcggc aagcgtgggc aggaggttgt tgcgctcgat
    36961 catgtattgt ttgcggtaga tttcgctgtt caatacggct gtacgcaaac tggtattgcg
    37021 ctcgagtgcg atgtcgatca gcttttgcag gcgcgggtcg gcaaaatagt catgccaacc
    37081 caaatcaacc gcgcggacgg aagaaaccga tgtgtcgttt tggaaggttt ccgcaacttc
    37141 gactttgggc tgctcgtatt gaggaatcat ggtgcaggca gacaatgcaa aggctgctgc
    37201 aacagaggtc aaggtagttt tcaatgtagt attcataaaa aagtcctgat gccgtctgaa
    37261 aaccggcggt tctcagacgg tattttgctt aatattgttt atcgtccgaa ccggttatac
    37321 ccgcttttga tgcatgtctg accgccattt catgctcgtg tgcagtttct ttgaagaatt
    37381 tgcgcaccac cacatagaaa agcggaacaa ggaacacgga caagagcgtg ccgatgagca
    37441 tcccccagaa tacggtcgtg ccgatggcgc gctggctggc agaactcgca ccagcggcaa
    37501 tatacagggg aaccacgccc aaaataaagg caaacgaggt catgataatc ggacggaaac
    37561 gcaggcgggc tgcctccaaa gcggcttcga ccgcgctttt cccttgcgct tgaaggtctt
    37621 tggcaaattc gataatcaga atcgcgtttt tcgcactcaa acccatcaca gtaacgaaac
    37681 cgacttggaa gtagatgtcg ttggcaaacg agggaacgct gcccaacagt ccttcaaaca
    37741 ggttgcgccc ggttacgccc gcagccgcac cgatcaaacc caacggaatc acaaggatga
    37801 ccgccagcgg aatcgaccag ctttcataaa gcgcggcaag taccaaaaat acggctgcaa
    37861 ccgccaaacc gtacaaaatc agggtttgcg agccgccttt tgcctcttcg cgcgactgtc
    37921 cgccccattc gaagctgtaa ccgccgccca attcgtcaac cattttttga accgccgcca
    37981 tagcctgccc ggtggaaacg ccggttgcag gcgaagcgga cagcttcatc gagggataac
    38041 cgttgaagcg tacgctctgt tccgtaccgt tttcccaaga aacagtagca atggtggaaa
    38101 gcggtacggc gacgccggat ttgttcggca cggtcaggtt caaaatatcg gcaggctgca
    38161 tacgggcatc ctcgtcggcc tgcaccatca cgcgttgcag acggccttgg ttcgggaagt
    38221 cgctgacata agacgaactc agcgcgcttg ccaatgcggt gcggatgtcg gcaaacgaaa
    38281 tgccttgcgc cgccgctgcg gcacggttga tgtcgatttt caactgcggc gagtcttcca
    38341 aaccgccggc acggacggtg ctggggtcaa acaaaccgct ggcacgcatt ttctgaatca
    38401 gctcgttgcg cttcgccagc aatgcggtat ggccggtatt gttgcggtct tgcaggttga
    38461 tgctcaggcc cgaaccgttg cccaactcca gaatcggagg cgggacgacg gcgatgccga
    38521 aaccgtcttt aagcgtcccc atcatcatac ccgtcagctt gccggcaacc gcaacggcat
    38581 cgctgccgga ggcggtacgt tcgttccaat ctttcaatat ggcaaaaccc atcgccatat
    38641 tctgaccgct gcccgaaaag ctgaagccgg aaacggtaat gatgttttca atttcgggaa
    38701 tgcttttcgc cagttgggta acttgtgcta gcgtcgcatc ggtgcgctct ttggtcgcac
    38761 ccgcaggcag ttgcacgctg accataacga agccttggtc ttcggtcggc aggaacgaag
    38821 tcggcaggcg cataaacagg aacacgccca caaccgccaa gccgatatag acaaccatca
    38881 tgcggaaagt cttacgcagc actttggcaa cccggccttc gtagccgtgc gtccaactgt
    38941 cgaatttctt gttgaaccag ccgaagaaac cttttttctc ttcgtgatgc cctttcggga
    39001 ttgtcttcaa cattgtggca cacaaagcag gggtaagggt cagcgcaagg aaggcggaga
    39061 atgcgattga tgacgccatc gtcagggcaa actgtttgta aatattgccg gccgccccgc
    39121 tgaacatcgc cagaggcacg aacacggaaa tcagaacggc ggtaataccg atgaccgcgc
    39181 ccgaaatctg acccatcgct tttttggtcg cttctttggg cggcaggcct tcacccgcca
    39241 taatgcgctc gacgttttca accaccacaa tcgcgtcatc gaccacgatg ccgatgacca
    39301 atatcatcgc aaacatcgtc aatacgttaa tcgacatccc catataagag atgaaggcga
    39361 aaccgcccaa cagggaaatc ggtacgacga tggtcggaat cagcgtataa cggatgtttt
    39421 gcaggaagag gtacattacg acaaacacca gcaccatcgc ttcgattaaa gtgtgaatca
    39481 ctttttcaat cgaaatttca acgaatttgg aagtatcgta aggggttttc caactcatac
    39541 cctgcggaaa gtatttttcc agcaccgcca agcgttcttt taccgccttt gccgtcgcca
    39601 tcgcattgcc gctgttggac aacattaccg ccataccggt ggtatttacc ccgttcaggc
    39661 gggttgagga agaatagtct tccataccca atccgacttt cgccacatcc ttcaggtaaa
    39721 tattagaacc gtcggtattg gcgcggagga tgacgttgcc gaattcttct gccgtaccca
    39781 actgcccttg cgccgttacg gtagccgtaa ccgtctgtcc gcgcacagcg ggaagcgaac
    39841 cgatggaacc tgcggaaatt tggatattct gcgccgacaa cgcgctgccg acatcggcaa
    39901 acgacaaatt gtagttttgc agtttcttag gatcgaccca aatccgcatc gcgcgttgcg
    39961 cgccgaacag gcggacctgc cccacgcctt cgatacgctg caattcggga acgacgttgc
    40021 gctgcgcgta gtcgttcatc tcttcggttg actgcacatc cgacgaaagc atcacaatca
    40081 tcaggaaatt ggaacgcgcc ttggatacgg ttacgccgta ttgctggacg gttgccggca
    40141 gcgtgctcaa tacttcggaa agcttgttct gcacttccac ctgcgccaga ttctcgtcgg
    40201 tatcgggcgt aaaggtcagg ctcacgctgc cgctgccgct cgaatcggcg gaagtggaca
    40261 tatagtccaa accttccacg ccgttcatat tccgttcgat cacggaaagt acgctgcctt
    40321 ccatcacttg tgcggacgca cccggataaa tggcgtgcaa agtaatggtc ggggcggcaa
    40381 cggacggata ttgcgaaacc ggcaggcttt taatgccgaa aatacctgcc gcaataatga
    40441 aaatcgaaat aacccacgcg aaaatggggc ggtcgataaa gaatttagcc atcgatgcct
    40501 tccttatttc gcttcagaag caggtttggc ttcagatgcc gtctgaacgc cggcttgagg
    40561 ggcggcagct tggttttcag acggcgccca ttctttaggc gttacctttt tcgcccccgt
    40621 cataccggcg atactgatgc cttccacaac caccttgtcc ccgtccttca gacccgacgt
    40681 aacaatccaa ttcgtaccct gctgttgcgc gaccgttacc tcgcggggtt ccataccgcc
    40741 ctgggcattc acaatcatca cggtatcttt cgcaccgcgc gttaccgcct gctgtggaac
    40801 gatgaatgca ttatccgccg ccacctgatc catcagcaca cgcacataca ggccgggcat
    40861 caggatattc tgatcgttcg atacggcggc gcgcaaggta atctgaccgg tcgattcgtc
    40921 aacggtcgga tcagcaaaca gcaggcggcc tttttcagga taaaccgtac cgtcgtcaaa
    40981 tttgatgccg accgcaatcg caccatccgc cgccagcagc ttgccttcgg ctatctgccg
    41041 gcgcagtttc atcacttcgg atgcagactg ggtaacgttc acatacatcg gattggtttg
    41101 gcggatggtg gctaaaacag ttgtatcgcc cgcattcaac agcgtacctt cggaaacttt
    41161 ggactgaccg ataaagcccg aaatcggcgc ggtaatgcgc gaacggttca gattgatgcc
    41221 ggcggatttg atcgccgcct gcgccgcttt aacgcccgcc tcggcagaac gtttcgccgt
    41281 taccgcagca tcgtactctt gtttactgat ggcatcggcg gaaaccagcg gtttgtaacg
    41341 cgccaaatcc gcatccgctt tggcaagcgt tgcctgtgcc gttgccagtt gcgcgcgcgc
    41401 gctttccaga cctgcttcat aagtggaact gtcgatctga tacagcggct gcccggcgcg
    41461 gacataactg ccttcttgga acaggcgttt ttggatgatg ccgccgactt gggcgcggac
    41521 atcggcggta cgcagcgatt ccaaacgccc cggcaactcg acggtcaatg cgacggtttg
    41581 cggatggacg gttacgacgc cgacgacggg cgcgggggct tcccgacccg caggctgccc
    41641 gccctgcgcc gcgtctccgc ctttaccgca agacgacagt gccaatgcaa cggcggcagc
    41701 caacgcggcc gcacgcatcg ccttagaagc ataaaaagcc attatttatc ctatctgtct
    41761 ggtttgatgt aaagggtttt gccaatcaac aggcattctt atttcaggat ataaaaaccg
    41821 cctgctttga tacccgaatg ttcgaacggg ttgcaaagca ggttatacct gttttcaaag
    41881 ttgagatgca gtctcaattt tatgggtttc attatacata cacgattgca cggataaaaa
    41941 gtctttttta taatccgccc tcgtcaaacc gacccgaaac gaaaacgcca ttatgagaaa
    42001 aaccaaaacc gaagccttga aaaccaaaga acacctgatg cttgccgcct tggaaacctt
    42061 ttaccgcaaa gggattgcgc gtacctcgct caacgaaatc gcccaaaccg ccggcgtaac
    42121 gcgcggcgcg ctctattggc atttcaaaaa taaggaagac ttgtttgacg cgttgttcca
    42181 acgtatctgc gacgacatcg aaaactgcat cgcgcaagat gccgcagatg ccgaaggagg
    42241 ttcttggacg gtattccgcc acacgctgct gcactttttc gagcggctgc aaagcaacga
    42301 catccactac aaattccaca acatcctgtt tttaaaatgc gaacacacgg agcaaaacgc
    42361 cgccgttatc gccattgccc gcaagcatca ggcaatctgg cgcgagaaaa ttaccgccgt
    42421 tttgaccgaa gcggtggaaa atcaggattt ggctgacgat ttggacaagg aaacggcggt
    42481 catcttcatc aaatcgacgt tggacgggct gatttggcgt tggttctctt ccggcgaaag
    42541 tttcgatttg ggcaaaaccg ccccgcgcat catcgggata atgatggaca acttggaaaa
    42601 ccatccctgc ctgcgccgga aataatcaag ccttggtaac aatgccgtct gaaacaaaca
    42661 aaccctttca aacggcatca aaatgacaca aagcattctt ctaaaaatac atattcacta
    42721 aattgcattt ttaatttccc ctatcatcgc atggatattc tcttggtcaa aatgtccgtt
    42781 ttcttctgaa taaacttcta acaaataatg ttcaatgaac gttttatctg tcatcaacga
    42841 tacatctttg gcaatgtctt catacgactc aaaatcatct tcatg
//
LOCUS       DAXFZR010000004        42005 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000004 DAXFZR010000000
VERSION     DAXFZR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42005
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.004"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1605
                     /gene="trbL"
                     /locus_tag="ACFJAS_000144"
     CDS             <1..1605
                     /gene="trbL"
                     /locus_tag="ACFJAS_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HFC3981507.1"
                     /translation="KKKYIYYFIFFITLSMPIISHAAGQGGVLDEALSRYEGKIGAWE
                     AAFYKAGLFIFWSLSSISIVMTGAQLIFQRDNISSFFAEFTRLILFLGFFLWIITNGI
                     KITTSIKDGLMKLAGQASRNGEQITPSSIVDIGFNLFDKIVENSSIWDPIDSATLIVI
                     GLVILLILAAVGINMLLMLITTYIVIYSGIIILGFGGGRWTSDMAVGYLKQVLNLSLQ
                     LAAMILIIGIGQSIVQDTINTIGTPGFRELAVVLVQTALLVGLVVKIPPMIGSLAGGA
                     GSGGIGSFGVGNAIAAAAMLAGAAAGAGAALKSLAVEAGGLKKAFEAAKAGLGGSNGS
                     SDSGSGKMDSAVDAVAGANAGDKSNQDGKPLTAESSGNSENGSEGSTTGNSGGNPSGS
                     SEENTISSGSSSSSGEANSTNTSASSGAEGSAEGEATSSETSQSASDSPSNNSGEATQ
                     TADTTPKTTNTGGGSPLATAMGGNKVSNWQAAKNVAVGLVSSKWNNAVNNSAGGRLVK
                     HWEDEKKKTEDPTKNPQKNPEGDDNA"
     gene            1598..2170
                     /locus_tag="ACFJAS_000145"
     CDS             1598..2170
                     /locus_tag="ACFJAS_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFC3981508.1"
                     /translation="MLKKTLICILLVGSVHFAMAESLNDALNRSRSGNSNNNRPQVED
                     LFTGDVKLACEATLCLSTGNRPSECQPAIHRYFSIRHKRMGDTIRARHNFLRLCPASK
                     EQGMLELVNAIANGAGRCDARELNRINRSTYIKRVWVGSRRHGDWQEVKVPYIRNAKP
                     SYCSAYFENGWTQLGTRYVGEEKNGGKWVD"
     gene            2170..2769
                     /locus_tag="ACFJAS_000146"
     CDS             2170..2769
                     /locus_tag="ACFJAS_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbN"
                     /protein_id="HFC3981509.1"
                     /translation="MVYVADLPPFMQERVTCSIAAAEKYHLPPHYLLAVAERENGRPG
                     QQVRNTNGTYDVGAMQINTAYIRTLSRYGVTQSAVAQPGCYAYYLAAWRIKRHIVYDR
                     KGDIWTKIANYHSYTPKYNAIYRAAIIRLAEKWERWLAGRQGNNAKGNVPTQAVPAVR
                     TIRSQPLPKQYVSPTTVYVPRTIRVVERSPEPEPETVQN"
     gene            2878..3162
                     /locus_tag="ACFJAS_000147"
     CDS             2878..3162
                     /locus_tag="ACFJAS_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFC3981510.1"
                     /translation="MAYAIAFDLDTTVLQELYPNSSWQNAYGDVKRTLVGLGFNHQQG
                     SVYFGNSEITAVSCVLAAQKLSQTYAWFKPAVSDIRMLRIEEMNDLQPAL"
     gene            complement(3272..3826)
                     /locus_tag="ACFJAS_000148"
     CDS             complement(3272..3826)
                     /locus_tag="ACFJAS_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC3981511.1"
                     /translation="MIIGYLRVSTKEQNVAAQRQVIQDKYQIERWYEDQAASGAVKAL
                     NRPEFRKMAEFVRQGDTVVVVAIDRLGRNTIDVLETVQFLQKKGVAVTSLRENFDLAS
                     PLGTAMLAIVSAFAELERSYIKERQMAGIRNAREEGKKLGRKVKVDPEEIRRWRQSEQ
                     TSISRTAAHFNVSESTVKRACKAK"
     gene            complement(3878..4309)
                     /locus_tag="ACFJAS_000149"
     CDS             complement(3878..4309)
                     /locus_tag="ACFJAS_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC3981512.1"
                     /translation="MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLA
                     DILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVRLSPFLNGCPHDLSLGAESLGKG
                     ESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ"
     gene            complement(4324..5529)
                     /locus_tag="ACFJAS_000150"
     CDS             complement(4324..5529)
                     /locus_tag="ACFJAS_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC3981513.1"
                     /translation="MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQH
                     ADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHNSAAVLSSTQFDEVLKRDEPQKK
                     EVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
                     VSMRAERASDNTYKRFNEYGRGASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQD
                     RNSEFIDKFDDVIKMLSLGSQEQILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESV
                     FAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGIYLLGQAKKAETGQT
                     YSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLE
                     YSAKKSISR"
     gene            complement(5536..5721)
                     /locus_tag="ACFJAS_000151"
     CDS             complement(5536..5721)
                     /locus_tag="ACFJAS_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981514.1"
                     /translation="MNKVEPQESNAIRMIKEACEKNRRMMTDEAFRKEVEKRLYAGPS
                     PELLAKLRVLWAANKEQ"
     gene            complement(5755..6963)
                     /locus_tag="ACFJAS_000152"
     CDS             complement(5755..6963)
                     /locus_tag="ACFJAS_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC3981515.1"
                     /translation="MIEYTPLSAEGKARILNGFMKPRLSRTQSVEQPKIVLVGAQPGA
                     GKSKAASLAKSELRQEGGYIHVDADIMRALIPAPEGVVYSSEQTQKDAGALAISVRNS
                     AKENRRNIVEEGTFRNAASISQFIRDRKSEGYGVEMLAVATASEESVAGIFKRYEEQH
                     AKGVSQPRFVEESYHNEAMAGFKDTLSQCESSFDRVRVTNRAGDILYDSLNRRQNQHE
                     TAKDALSAYQEITPKRLKQVVKAWDEIQLQAESRSIDPIPNYLGMVKQHSEAIYQRVE
                     EIYRQERVVANSEGATLQRKSGDTWQDIEKAEAKGMKAGIHMLGTAKPAKSGREYSGE
                     IVHKDEASVFQKTDQGLIRHKAVQGMAEGKFSSLSEQVEIGQKVSIKREGNELSVKPA
                     DASLKKTMKR"
     gene            complement(6960..7214)
                     /locus_tag="ACFJAS_000153"
     CDS             complement(6960..7214)
                     /locus_tag="ACFJAS_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981516.1"
                     /translation="MQMQETIPGINYEVALELLGDSMRPLIQARESEFVKDKPNQAFI
                     EYCTARLEAIDMLRDSLSPEDTDIIARIFNPDDQTVGRVK"
     gene            complement(7591..8112)
                     /locus_tag="ACFJAS_000154"
     CDS             complement(7591..8112)
                     /locus_tag="ACFJAS_000154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981517.1"
                     /translation="MNMKTVQEYEIIIRLTKENGRYGAAAETVSGGLLESVPPLSAAG
                     NDALYALLHALALKNNFSGCLKEAQRRESVKNIEAKIEEKMKPIELQYYFSDMNVGEF
                     NAYLTVKNLAKPTFISHIDIDEDDKMFIANLAKGIASEYKSLFYLDVNRQTIEKALLR
                     YLEVCVSCNSAQD"
     gene            8500..8616
                     /locus_tag="ACFJAS_000155"
     CDS             8500..8616
                     /locus_tag="ACFJAS_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HFC3981518.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8632..10551
                     /gene="tet(M)"
                     /locus_tag="ACFJAS_000156"
     CDS             8632..10551
                     /gene="tet(M)"
                     /locus_tag="ACFJAS_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFC3981519.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDPVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQKYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10643..11116
                     /locus_tag="ACFJAS_000157"
     CDS             10643..11116
                     /locus_tag="ACFJAS_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981520.1"
                     /translation="MPHEQMIDAFSAIIQNSLSLYEEQTPLYYYLTICGSWVLTALGI
                     LSLASVFFTIKQKKPYRFIVPILFLWLGYESIHMPRPNLAVFYGYNLAQLSYPANENP
                     NSFLHLADGSNPICCFIGEDEYLQAKRLGFKEYADAKAKALVDLVEMEKKSKQSD"
     gene            complement(11268..12164)
                     /locus_tag="ACFJAS_000158"
     CDS             complement(11268..12164)
                     /locus_tag="ACFJAS_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine modification methylase"
                     /protein_id="HFC3981521.1"
                     /translation="MFTHSVCSYPQRGNYGKSGYRGNCTGHIIRDFVESYLAPEGLFA
                     DPSIGSGTSSDVAEEMGIRFKGTDLHQGYNLLTQDFAAFLGEQANLIWWHPPYWNMIR
                     YSGNQWGDAANPWDLSQMDLEQFQEAMLLAMMNIHDATEQGGHYGILMGNMRKDGRYY
                     NLSSMVERMAAGRLVDEIIKIQHNCVSDSRQYGGKLVRIAHEKLLVFKKEIQTALYFL
                     AKAMNRVEAVTAVTWKAAVRRVLQAADGRNMGLTEIYGEMEPYAAARAENRNWQAKVR
                     QVLQDERFFLRVGKGIYALKTE"
     gene            complement(12168..12509)
                     /locus_tag="ACFJAS_000159"
     CDS             complement(12168..12509)
                     /locus_tag="ACFJAS_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981522.1"
                     /translation="MLYWDDVSDSFDGWMNLLRQLGGDSFINFEQDIWETARTYETVP
                     HFGNLRQHHLLERLQDTIRNRWPFLRTGFLINALDTHFYVNDEPVETMRDLDAVLGCH
                     YRENEDDLKEE"
     gene            complement(12523..12837)
                     /locus_tag="ACFJAS_000160"
     CDS             complement(12523..12837)
                     /locus_tag="ACFJAS_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981523.1"
                     /translation="MASYTNRLTGHPNVFVEQNIWSNGELMGFSPINVMWDGRNAPTL
                     LCRYTFDGGQYYSLQVSEAAELETRGYQIVCDDLQCLKLKTAKARRSGILALILADAG
                     IE"
     gene            complement(13204..13563)
                     /locus_tag="ACFJAS_000161"
     CDS             complement(13204..13563)
                     /locus_tag="ACFJAS_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981524.1"
                     /translation="MSLSAENRTFYLVETGEIGFGSSALFISQSLEEAEAVVADFFKG
                     DEKLHYETDGNQPEISLGMGEPQEEEYIPFLPREPFFWTKGGSGMMGTEYVTIRKTIN
                     GYLEGAGSFRLIKSQQD"
     gene            complement(13560..13994)
                     /locus_tag="ACFJAS_000162"
     CDS             complement(13560..13994)
                     /locus_tag="ACFJAS_000162"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC3981525.1"
                     /translation="MSIYRPVSRERKNIKFFADGQYIQEGYKALCWMMRDVVDNHQMH
                     AININLINLLFAQQQYLRDLGRPNPELVLHSGFRTRRHNDSLEGAAKNSQHLSGNAGD
                     FHIERASLSELAALARRFRVGGIGIYPTFIHNDIGVYREWRK"
     gene            complement(14067..14120)
                     /locus_tag="ACFJAS_000163"
                     /pseudo
     CDS             complement(14067..14120)
                     /locus_tag="ACFJAS_000163"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
     gene            complement(14117..14587)
                     /locus_tag="ACFJAS_000164"
     CDS             complement(14117..14587)
                     /locus_tag="ACFJAS_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
                     /protein_id="HFC3981526.1"
                     /translation="MAFFLTYHKQAREGWFWRVFGMAFDGRLRQTLQNIAVDYRKTSQ
                     NQPLAFDDYLEMLQRHEEEKMGVSAERNQSEQAAVKAFQKQYGLNHRVDTHADTKTLS
                     ITITATDEDFIPAVEMGLEQVLGSLAAGETANEGGNEYGRYAFMVSGNVKGRER"
     gene            complement(14620..17619)
                     /locus_tag="ACFJAS_000165"
     CDS             complement(14620..17619)
                     /locus_tag="ACFJAS_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFC3981527.1"
                     /translation="MQNKKPFYEQVAEKLIEQLRQGTAPWQKPWDGGGLPDFMPHNAI
                     TGRRYRGINNLWLQAQGRKDPRWITYKQASSIGAQVRRGEKGTVVEYWQFTEEVPKTD
                     EKGQEIIGQDGKPEKETVRLERPKVFHAVVFNGEQIDGLPPLKLRPLSWNPDERAENI
                     LKNSGAVIDHRHGDRAFYSPSRDSITLPLREQFESPGAYYETALHELGHWTGHADRLN
                     RDLSHPFGSEGYAREELRAEIASLMLSQELGVSFNPGQHASYVASWIMVLQDDPMEIM
                     RAASDAEKIQGYVMAFDQVREIEQQNEAAVSISDRQNIDEASLAEEKNLINAVDKNDV
                     AAIINFMAEEKMHEGLKRFSLVISEMDDATFQNAFWQRHQKPENGESFMENLNAAKEA
                     LDEAIEQSSYRTVEEQIRQFLPEQDKESVRVVSDERVYVQIPYADREQAKMLGAKWDK
                     EEKSWYIPPGVNQEPFRKWLDSATNQAKTVTDNTPAIAKTLIDVPFREKNEARALGAK
                     WDKDQKSWYIPEGVNTALFARWMQPKQEALAEEAVPDDTPQEEAVECQVAAIQQEYQA
                     AKTEEAPAEENASRVYLAVPYEEKEEAKALGAKWDKANKSWFMESSNANLPAASKWLP
                     EKQQTQSMPAMSPVEEFADKLREMGCVVANGHPFMDGQKHRIETVGDKKGERSGFYVG
                     HLDGHPAGYIKNNRTGAEVKWKAKGYSLSPEEKAKLAAEAAQKLQMRSEQKKQAQEAA
                     VGRIKYYMESYLPVQTGTPYLNAKGIKPQPGIFTDREGQKTFIPVYNAAGELRSMQYI
                     QADGTKRFAKDSEQEGCMHVVGQQDLAKAKTIILSEGYATAASIKEATDDTVASVAAF
                     NSGNLPLVAKVLSAKYPQAQFLVAGDDDLAVEAKQGNNPGKEKALEAAKILNCRAVFP
                     VFAPGEQSSEHKAFTDFNDLAQKSKFGREGLAKQINEAIHARPANELQTLKTRGLQEE
                     ASQDRPVEQTKRQQRATTRKTASRGSR"
     gene            complement(17622..17804)
                     /locus_tag="ACFJAS_000166"
     CDS             complement(17622..17804)
                     /locus_tag="ACFJAS_000166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981528.1"
                     /translation="MSIDIEVIAEKVADLNNPGMEVDFDPAEAEALGAFEETAMDAET
                     ARDSVADLSREVAEGA"
     gene            complement(17808..20003)
                     /locus_tag="ACFJAS_000167"
     CDS             complement(17808..20003)
                     /locus_tag="ACFJAS_000167"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC3981529.1"
                     /translation="MSRLYIAEKPEVARAIAAALGVGIERRNGYFRCSNGDNITWCYG
                     HMLALCDPEDYDSKYGNWKFEDLPIAHIPWRKKPLSDKKEQIGVIRDLLAECTEIVHA
                     GDPDDEGQLLVDELLEFLNCAKPVKRVLINDNNERSVKRALSAIEDNAKFRGLSQSAE
                     ARSVADQLYGYNMTRAYTLKAAEQGYQGVLSVGRVQTPILGLVVRRDRENQSHVKTHY
                     YHVYADLDVDGVRFTARYVPNKDQDPIDDKGRLSDPLFARQIAEAASGQPVKILAAKT
                     SAKRQSPPLPYNLLKLQADCSRKFGLKPDQVKDIGQSLKDKHRLITYHRSDCQYLNDE
                     HFDDAADVLDAVGKTASVFKNAIASADTKIKSRAFNPDKVSAHHAIIPTVKTANMDDL
                     TEDEKRVYLLIARAYIAQFWPDHEYNQTDIEAEIAGKKFVCRSNVTVKPGWMALYKND
                     QGNAETATDPEDLSVDLQMLKPGNSGRCLTAEETQFETKPRPLYTMDTLLNDLTRVAK
                     YVQNPKLRELLIEKDKGKEGEHGGIGTPATRDEILRNLLDKGFLLEKGKNIVSSEIGQ
                     QLYDALPDQAKFPDMTALWHEQQQHIVQGGLDAQRFVNSLMEYISGEVERVGREGISL
                     NVKKYPCPECGKPLRRMRGSKGFFWGCTGYKDGCKVSFSDKAGRPVLAPKKAEPSSKF
                     KCMACTHPLVRRPGKQKGTHFWGCSNYPECKQYYPDAGGKPDYSKHTKG"
     gene            complement(20006..20542)
                     /gene="traF"
                     /locus_tag="ACFJAS_000168"
     CDS             complement(20006..20542)
                     /gene="traF"
                     /locus_tag="ACFJAS_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HFC3981530.1"
                     /translation="MRRLFDRAMLAMCLTAALLVTGYGLLYAAGFRINDTRSVAKGVY
                     RISGEAVRRGSYITFCPPDTEVFRMARRRGYIRAGFCPENYSGLMKFVAGIPGDNYRF
                     TDEGLLINGRLLGGTRPLWQDGLGQPLPVFEGEGRLKDDEYILIGNAVANSFDARYYG
                     IVRREHIRDVVKPIWIEE"
     gene            complement(20539..22428)
                     /locus_tag="ACFJAS_000169"
     CDS             complement(20539..22428)
                     /locus_tag="ACFJAS_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFC3981531.1"
                     /translation="MFRSKNKRNNAVGHQNQAQATGSGRLVNFICLVLVLLAGMGAAT
                     QYFAHQFQYHSQLGYGIGKFYMPWSILIWYGKWHGRYEQQFMTAFSIMLMVVAVGMIG
                     LLVWNIVRKNTNKAHESLHGSARWADRKDIEAAGLLAKKGEDSDGVYVGGWVDKKGTL
                     HYLRHSGPEHVLTYAPTRSGKGLGLVLPTLLSWPYSTFITDLKGELWALTAGWRKKYA
                     HNKVIRFEPASAQGSAAWNPLDEIRVGTEYEIGDVQNLATMIVDPDGKGLNSHWDKTA
                     FALLTGVILHALYKARDDGGVATLPSVDRMLSDPEKPVGDLWVEMATYPHADGRPLPV
                     ISSAGQDMLDRPEEEAGSVLSTAKSFLALYRDNVVAANVSRSDFKIKDLMNDEDPVSL
                     YIITQPNDKARLRPLVRILINMIIRLSADKMDFENGRPKVHYKHKMLAMLDEFPSLGK
                     LEIMQESLAFIAGYGIKCYLICQDLNQLKSRETGYGPDETITSSCHVQNAYPPNRIET
                     AEHLSKLTGITTVVKEQITTSGKRVSAMLGQVSRTTQEVQRPLLTADECMRMPGPVKD
                     ARGLITQAGDMVIYVAGYPAIYGRQTLYFKDPVFEARSKVPPPRHTDRTQKKTANTPS
                     AFDTL"
     gene            complement(22445..24838)
                     /locus_tag="ACFJAS_000170"
     CDS             complement(22445..24838)
                     /locus_tag="ACFJAS_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFC3981532.1"
                     /translation="MIAKKIPMRKANLSSFEGLVTYLAGSQGVANRVGKVEIHNYESS
                     DLRWAVQETLAVQDQNKRSAADKTYHLMVSLAGGEFLTDEQLSRIEQSMIEALGYAEH
                     QRISVVHRDTDNLHFHVAINKVHPEKKTIHEPYNDFKILGRECRKFEEEYGLQRVQRG
                     GRGNKKIDMEEVAGIESLIGWIRRECGEQIKEAKTWEQLYHVLSDHGLEIKLRGNGFV
                     ISDGEHHVKASSVDREFSKSALEKRLGAFDQKTGVKAKPSKTYREKPLSKANVDDLFR
                     QYKEEKELQKSRRQDFFLHLAQNRDMEIGRIKEKYAFRRSLIRHSSSGIARRILYRML
                     FRQMKDEIRSVRTDYARRRAVFFEQNKGFAWADWLKHRAAEGDVRALDALRRRGKDAG
                     YKGNRFVGKESGGFAGIKVDTVTKKGTVIYAEDGMTIRDTGSVLKLAAIPDDKAVSRA
                     LEIAIGRYGKVLTIGGTRHFQDKVARIAAAKGLDVSFTDGRLNHLYQQYVKEYEYVRE
                     RTDRYRQADGTGRADGRAGQQRGGAGYAGGKPRRYRWHGYDASRNRGFHAGISGYPIP
                     KLSDSGGIGAGRGQSASAGSDSLRGMPERSLVRIQGRTQVLLPRDVSSHLEQQGSANY
                     QAVRRTIHRTGGVAEIRQMQAVVGLVAKPGKKPPGFRRGSLRGMEGLAEIKISSAAVV
                     EIPKNPEPAQENERLTHSLAPIDKYIAERQEKIDKKFDILNHRKYNPSDSGVMLFRGI
                     RNIDGQALALFQPKEVEEILVMSVTPYVSRRLSSVKLGSEVKVTPTGQIRPVKGRHR"
     gene            complement(24841..25218)
                     /locus_tag="ACFJAS_000171"
     CDS             complement(24841..25218)
                     /locus_tag="ACFJAS_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFC3981533.1"
                     /translation="MAGARKRPKYISVYVSEEEWKTIESLAKSVGLSQSAYLKLLGLG
                     YKPASVYDGEAVKALAKINADQGRLGGLIKMWLANDERLMMFDHDQLAKAIFGVLEQI
                     GEMQESMLEIVRSRRILAAKKIG"
     gene            25268..25969
                     /locus_tag="ACFJAS_000172"
     CDS             25268..25969
                     /locus_tag="ACFJAS_000172"
                     /inference="COORDINATES: protein motif:HMM:NF036299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HFC3981534.1"
                     /translation="MASLAEQDNPLSLPANKDCDVHVHHISCPNSGVQYAKDYTPNNS
                     IYRTYFDDKPPKNALSQTKNDLLYRIIVFKTILLGSFGKALSKNWQSPIEMIQMGKIE
                     KLVLAELAAMQKKGSSAGNKARFLAMQEDIKILLVDRNIPVISVWRILSEKKYVPYSY
                     ETFNRYCKMYIFKSDTTAADQTDTAQSTQEKPDSIRTDKEPPSPASEPIIAGGNTKSS
                     SGFTFNNNPDGKDLF"
     gene            25970..26695
                     /locus_tag="ACFJAS_000173"
     CDS             25970..26695
                     /locus_tag="ACFJAS_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HFC3981535.1"
                     /translation="MAIIHISLQAKGGVGKSFLALMLSMFYRHIERPVKIFDTDPANA
                     TLFGFPALEAEKFEIMKKDDIDKRLFDRLFSQILSATDDVIIDVGANAFIPLCSYIIS
                     NNLITMLTQEGHQVIIHTSIVGGQNLKHTVNGFDALCSQFPESTGFVIWLNPFWGEIE
                     FEGKSFEKLKVYEKHKSRIRSIIKIPEWDASLHGRDLAAMLEKQKTFDEALASEDFSI
                     FERQRLNIIKNEMFQTIETAQIV"
     gene            26703..27185
                     /locus_tag="ACFJAS_000174"
     CDS             26703..27185
                     /locus_tag="ACFJAS_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981536.1"
                     /translation="MDEKVKAILATIVEKNGIALSPKDPIMVVFTALEYMLSEQHAEQ
                     KALTVLFKAQVEEATGNWIQSVHQQTEELVSQQLNTTKEQAKEMSKLLLHETDRLLEQ
                     RITDAVKDFEGKLYGINDNAMNAVELRIKETRNTAILNLTAALIVLLAVIILAFKFTF
                     "
     gene            complement(27242..27778)
                     /locus_tag="ACFJAS_000175"
     CDS             complement(27242..27778)
                     /locus_tag="ACFJAS_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncP plasmid survival protein KfrC family
                     protein"
                     /protein_id="HFC3981537.1"
                     /translation="MNEDKGLVAKAVAAETEQEELFESRQVIEETFAGVLNTYVLEKT
                     EQVERIEEKLEQLISGQMAKLQALQNARPGMLSMPSKKQTWSANIAVAQSRLNRLHDR
                     LEDVREIRDGMALGVPKIQELAARKLRMEEPDLAKKFDDVQTAVRVHNLHEKQRKEKE
                     QQKRQAGLSLSLGQKIVR"
     gene            complement(27815..28171)
                     /locus_tag="ACFJAS_000176"
     CDS             complement(27815..28171)
                     /locus_tag="ACFJAS_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KfrB domain-containing protein"
                     /protein_id="HFC3981538.1"
                     /translation="MEAEKQRVLVLNKQHLFETEKDGKWITQAVKPAPANMKPGIYLL
                     HTAKSPQDSVRYEGQIVFQNDSYVYQKTRQSIVKYPCSLFSSVPSIGEVVSISVKDKA
                     ATVEKAGIGEKKTLTR"
     gene            complement(28378..29295)
                     /locus_tag="ACFJAS_000177"
     CDS             complement(28378..29295)
                     /locus_tag="ACFJAS_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC3981539.1"
                     /translation="MNNSNLGLENIGNLSELLGNAEVSSGTPLRLPLASIKEDPDQPR
                     KEFDEGKIQELANSIQERGVKTPISVRQSSEEGVYVINHGARRYRASLLAGLSEIPAY
                     IDQDYSLIDQAVENIQRDNLTPREIADVIGRLLAEGMKKGQIAEKMGKSPAFVTQHAN
                     LLDLPEPLANAFNSGKVSDLTVINDLNALYKKEPEAVEDLLSNEDEITRSSVRNLKEF
                     LNSDPKNSADADHEKPSKAKVKEKKPEDPDKLKKAIVQVMYQGQLARLVLNKRGLSGD
                     EVWIKFEESGETESVPCKEISGIVAVIEG"
     gene            complement(29301..30056)
                     /locus_tag="ACFJAS_000178"
     CDS             complement(29301..30056)
                     /locus_tag="ACFJAS_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC3981540.1"
                     /translation="MKILVVANQKGGVGKTAMSLHLAWHMETVGLRVLVIDLDTQGNA
                     SYSLRDKTCLFGSGRLFGNMEDADLHMPGNVSLALSPATNDLANVQNMTLQNAVQSFT
                     QNIDKLKAGGQFDVCIIDTPPSLGNTLAAALAAGDYVLCPIELETYSLQGIKQMAATI
                     GNIRKVNSKLAFLGILPSKVDMRNPRHKRHLEEIKMQYNEIVIPHIIGLRSSIADALS
                     SSLPVWKIKKTAARKAAQEMKAVSNYVLNKMQG"
     gene            complement(30393..30740)
                     /locus_tag="ACFJAS_000179"
     CDS             complement(30393..30740)
                     /locus_tag="ACFJAS_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981541.1"
                     /translation="MTDLISTTVLLTPYQAFQAKKWHLQNIEEHNLIQKKDLNIRPEC
                     FHRFPQWAVPTADEVKELWLATGMTQKQVSRYLGLKDTNGRAFRRFISGESSITYAYW
                     VLLSLYVGIEKFW"
     gene            complement(31358..32236)
                     /gene="trfA"
                     /locus_tag="ACFJAS_000180"
     CDS             complement(31358..32236)
                     /gene="trfA"
                     /locus_tag="ACFJAS_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HFC3981542.1"
                     /translation="MKNVQEVLPYLNLVAERRDEAQREEYLKSKGIPTDDNLIIEDLF
                     GNTIFGSKHRAMPNDFARSCLFTARNHRVPRKTFSREKLFHISEKVEILYTGTELRSV
                     DDELIWMQLVDYCRRFPLGNYVEFDIRQLIKDIGWDTSGAYYKRVRESLSRMKATEIY
                     IKNGNTYGVSGGISLIDNYVGIENIKGEPTRYKVSIDKKLIVLFAGNTFSNIPWSQYK
                     KLSPMARRLTDYVFSHKHPNPIPIPAFLAMCGSDQINSPAKTQNQNAKKICAELTEAQ
                     LVKTAFVLSGKIIVER"
     gene            complement(32240..32623)
                     /locus_tag="ACFJAS_000181"
     CDS             complement(32240..32623)
                     /locus_tag="ACFJAS_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC3981543.1"
                     /translation="MNKFIISGRTTKDLTLEQTATGVFTTSIPLAENYFYTKDGKKVE
                     ATNYFWIDVYGEQAKNAVRYCGKGSLILIEGRISNNNYEKDGQKIYKDQMIASRIEYS
                     NLKPPAGREESSDPSFIYDPEQDLG"
     gene            32683..33048
                     /locus_tag="ACFJAS_000182"
     CDS             32683..33048
                     /locus_tag="ACFJAS_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC3981544.1"
                     /translation="MSYSKIFFTNVLRLASEKNLSHQDLAERANLSPSSMSGITRGQG
                     NPTLETMSAIAYALDVPLSYLLEHHDLDPESLSLLDGSQSTLPPNYEKITVILPRYKA
                     FQVKKWNDDALKILAKETK"
     gene            33177..34175
                     /gene="trbB"
                     /locus_tag="ACFJAS_000183"
     CDS             33177..34175
                     /gene="trbB"
                     /locus_tag="ACFJAS_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HFC3981545.1"
                     /translation="MMETTETADSRKERALNKLKRDLGNEFLEKFMNPNLVEVMFNPK
                     QTGNGVSVYWEEQGKEMVYDERLSDWFKTSRVEAVIKTVAGYHGKVITEDLPILESQF
                     PIDGSRFTAILPPVTASPSFTIRKKPARVFTLDEYVSAGIMTESQRQTIRNAVSEHGN
                     ILVIGGTGSGKTTLINAILYDMSEQNPNERLYISEDTGEIQCHSQNKVQVYTTGSIDL
                     TKLVKTALRYRPDRIIVGEVRGAEALDLLDIWNTGHPGGIATLHANNAALGLERLESL
                     ITRNPAHPAAIRPLVAQVIHYIVSIGKDEKTGRKIREIIKIKGLDEKSGNYVFDYL"
     gene            34277..34660
                     /gene="trbC"
                     /locus_tag="ACFJAS_000184"
     CDS             34277..34660
                     /gene="trbC"
                     /locus_tag="ACFJAS_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer system pilin TrbC"
                     /protein_id="HFC3981546.1"
                     /translation="MTLSQSQQKWLFFIGLAVFLLLMSSPALASQGQGGGLPYESWLN
                     NLRQSVTGPVAFALSIIGIVVAGGVLIFGGELNAFFRTLIFLVLVMALLVGAQNMMSS
                     FFGGAEVTAAVQAAQHYLLPLASKG"
     gene            34665..34982
                     /gene="trbD"
                     /locus_tag="ACFJAS_000185"
     CDS             34665..34982
                     /gene="trbD"
                     /locus_tag="ACFJAS_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFC3981547.1"
                     /translation="MRAIRTIPIRRVANRPNLFWGGDREMVQVSLLITFVLVVAAQNT
                     YAFVIGLVFWFFALYALRKLAKYDPLLRFVGLRSITKYKRFYPPHSTPFRNNTSTQGN
                     RYK"
     gene            34979..37543
                     /locus_tag="ACFJAS_000186"
     CDS             34979..37543
                     /locus_tag="ACFJAS_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HFC3981548.1"
                     /translation="MIIGIISAISVLGLVLCTGLFLKIRAVTNATKLKQHRSKEEGLA
                     DLLNYAAVIDDGVILGKNGSLMAAWIYRGKDMGSSTVAEREDVSLRINQAFVGLGSGW
                     MVHVDAARRPAPNYTEAGRNYFDSYVTEAIDNERRQAFEAIGTLYEGFFVIVATYFPP
                     LLAEKKVAELMFDDDNAKEGKRAQQDRTLSHFKQACANLESNLSISLELHRLCAHKVV
                     LEDGMEITQDDFLRWLHFCITGKNHPINLPAAPMYIDSLIGGQELYTGVVPMIGDKFI
                     QVVALDGFPMESYPGILTRLGEMPIEYRWSNRFIFMDQHQAVADLEKYRKKWKQKVRG
                     FFDQVFNTHSGSIDQDAQAMVDDAEAALAETKSGLVAQGYYTSVVILMHEDRAFLESM
                     ARQVEKEINTLGFNARVETVNTMEAWLGSLPGHGIENVRRPLLNTLNFADMIPTSSIW
                     TGLEENPCQFYPPNSPPLMQVVTSGSSPFRLGLHVGDVGHTLVFGPTGAGKSTLLATL
                     AAQADRYRNMRVFSFDKGRSLETLTRAVGGSHYNIGDEDNTASGLSFAPMQLIQNTSD
                     RAWALSWVEAVMELNGLKVTPQQRNEIETAIKNNAAEQSYSLSDLSNTLQDVEMREVL
                     KAYTIDGSMGYLLDAEQDSLSFSRFTCFEIEELMNLPEKYALPIMLYLFRRIEKALTG
                     DPAVLFLDEAWIMLGHPVFREKIREWLKVLRKKNCLVLLATQSLSDAANSGILDVLNE
                     SCPTKIYLPNAHAREEDASELYRRMGLNARQIEILATAIPKRQYYYTSPYGRRLFELA
                     LDPLTLAFVAVSDPDTLNAMRQTADQYGEDWPIRWLQNRGINIHNYVDVDKYRRKH"
     gene            37543..38250
                     /locus_tag="ACFJAS_000187"
     CDS             37543..38250
                     /locus_tag="ACFJAS_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HFC3981549.1"
                     /translation="MGLKDIFRVREKADREMTENPYLNARKQWNFIMGDMVSSRLLWQ
                     WAGMTGLLIGLGGVGGMIYLGSQSKFIPYIVEVNKLGETVAVARADQPQATDERIVRA
                     MLGSFIGNARTVTTDIALQRKYVLNTYAMLNPKDAAAAKMNEWLNGTPESSPFKRAEK
                     ELVSVEIKSVLRQSDNSWQVDWVETTRAHDGKLLKPPQNMRALLNIYYVPPTTEQQII
                     VNPIGMFIRDFSWSNEL"
     gene            38263..39129
                     /gene="trbG"
                     /locus_tag="ACFJAS_000188"
     CDS             38263..39129
                     /gene="trbG"
                     /locus_tag="ACFJAS_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HFC3981550.1"
                     /translation="MKRQILSLALITALSTSAFADSYADKYFEGSHNPKLNRHEQKGL
                     GIAKNYSSDGRMAVPPVPGDNGRITFAFGSDPSVVCAVMQVCDIALQPGEMINDLHAG
                     DTARWNITPAISGSNGNERMHILVKPLDVGLQTTLFIATDRRTYHIRLKSHRTRYMPA
                     VGFSYPEDTAARWRAIRANQARQAEAQVLPTGENIQNLDFNYTISGHNPSWKPLRVYN
                     DGTRTIIQMPPTLSQGEAPALLVVRNGENTLVNYRLHEDRYIVDSLFDEAIMITGVGR
                     HQTKVVIRRSRS"
     gene            39126..39536
                     /locus_tag="ACFJAS_000189"
     CDS             39126..39536
                     /locus_tag="ACFJAS_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbH"
                     /protein_id="HFC3981551.1"
                     /translation="MKSNVLFVALALAVGGCATGQYGSFVRSDAVSASVTQDVVEKLQ
                     EEFPAANTVFFLNRSKSDTFGTQLDQALREKGFATTAQQGENEKSLNYVLDQVDSDIY
                     RVMIYIDGTPYSRAYRQTENGAVVPTGSWTKRGD"
     gene            39540..40979
                     /locus_tag="ACFJAS_000190"
     CDS             39540..40979
                     /locus_tag="ACFJAS_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC3981552.1"
                     /translation="MSQSTEDLLNASRSPSIAPDTSGRRPGVRRLNNIPIYIVGGVVL
                     GFALLIALVAATKGQQSAGQATQGDGKDATRMADSLLNELPQEGGLITAKASEPAMPA
                     LPDGMASAPDLPASAPTSLPMVPDMANLPATPPEVDQEAEQIRQAKAQMFQAALAAPT
                     KVDVVASTSRGSSGLYTAALSSSGSGTPNRSEMLAQIAEVQRMSENAGSNPAEVERVY
                     NQQLANAQQMAAAGGQDGVQGAGGMPGLNTPPTPGNIPRQTAQNGGNNWRLESQVERP
                     NAYMIRSGAVIPATLISGINSDLPGMVQAQISQNVYDTATGRHLLLPQGSRLVGTYSS
                     GINLGQRRLFIAWNRIIFPDGKALDIGDMPGASGAGYAGFSDRINNHYGRLWGNALML
                     SLVGASITYGVDKNSNNNNNGNNNGNNSTVRGALSESLGQTFGQAVSQSIQKNMNVSP
                     TLEIRPGYRFNVILTKDIDFSKPYRSFDY"
     gene            40992..41768
                     /gene="trbJ"
                     /locus_tag="ACFJAS_000191"
     CDS             40992..41768
                     /gene="trbJ"
                     /locus_tag="ACFJAS_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFC3981553.1"
                     /translation="MQKRLLSKTKTVALSLAVSVSLGLSAPAYAGIPVTDGGNIAQSS
                     VSAIQNVAQTLKQIEEYRIQLKQYETQLRNSLAPAAYVWDQANKTMANMKAAVARLRA
                     YKQQLGNMEEYLSKFQAVDFYKSSPCFTAKGCSAADRAKLEQNYAQLSRMQKLSNDGL
                     LRSINNEIDSISRDANTLTQLQRNAQSATGQLEALQYANQFHSAQAHQLLQLRNMINT
                     EMQANAAYRQSQLDREARYEAARKQVRTSDILKHPYRRGQ"
BASE COUNT        10537 a        10916 c         9226 g        11326 t
ORIGIN      
        1 aaaaaaaaat atatttatta ttttatattt tttataacgc tctctatgcc aattatctct
       61 catgcagcag gtcaaggagg agttttagat gaggcattat cacgctacga aggtaaaatc
      121 ggagcgtggg aagcagcatt ttataaagct ggattattta tattttggtc attatcttca
      181 attagtattg taatgactgg agcccaactg attttccaga gagataatat cagcagtttt
      241 tttgctgaat ttactcgttt aattttattc ttaggatttt tcctttggat tatcacaaac
      301 ggaattaaaa ttacgacatc aattaaagat ggtttaatga aacttgcagg acaagctagt
      361 agaaatggtg agcagattac acctagtagt attgttgaca taggttttaa tttatttgac
      421 aaaattgttg aaaatagctc aatctgggat ccaattgata gtgccactct tattgttatt
      481 ggtttagtta ttttactaat cttagctgct gtcggaatta acatgttatt aatgttaata
      541 acaacctata ttgtcatcta ttcaggaata attatcttgg gattcggcgg tggacgttgg
      601 acatcagata tggctgtcgg atatttaaaa caagtattaa acttatcttt acaattagca
      661 gctatgattc taattattgg aataggtcaa tcgattgttc aagacactat aaacactata
      721 ggaacccctg gatttagaga actagcagtt gttctagttc aaacagcttt acttgttggt
      781 ttagtcgtta aaattcctcc aatgataggt tccctagcag gaggagcagg aagtggagga
      841 attggctcct ttggtgtagg taatgctatt gctgctgctg ctatgttggc aggtgctgct
      901 gccggagcag gtgcggcatt aaaaagctta gccgtagaag ctggtggttt aaagaaagca
      961 tttgaagctg caaaagcagg cttgggaggt tcaaatggct catcagattc aggtagtggg
     1021 aagatggata gcgcagttga tgccgttgca ggtgcaaatg ccggagataa atctaaccaa
     1081 gatggaaaac cactgactgc tgagtccagt ggtaactcag aaaatggtag tgaaggatct
     1141 actacaggaa attcgggagg taatccatca ggctcctccg aagaaaatac aatttcatcc
     1201 ggaagctcct caagttcggg ggaagcaaat tcaacaaata catccgcttc atcaggagcg
     1261 gaaggtagtg ccgaagggga ggctacttca tcagagacat ctcaatcagc ttctgattcg
     1321 ccaagcaata atagcggaga ggcgactcaa acggctgaca ccacaccaaa aacaactaat
     1381 accggtggag gaagtcctct tgctactgcg atgggtggta ataaagtatc aaattggcag
     1441 gcggcaaaaa acgttgctgt cggccttgtt agcagcaaat ggaacaatgc cgtcaataac
     1501 tctgccggtg gccgcctagt caaacactgg gaggacgaaa agaaaaaaac agaagacccc
     1561 acaaaaaatc cccaaaaaaa ccctgaagga gatgacaatg cttaaaaaaa cgcttatctg
     1621 catattgctg gtcggttccg tacactttgc tatggccgaa agcctcaacg atgcgctgaa
     1681 ccgcagccgt tcgggaaact cgaacaacaa caggccgcag gttgaagact tgttcaccgg
     1741 tgatgtaaag ctggcctgcg aagccacgtt atgcctgtcc acgggcaacc ggccgagtga
     1801 atgccaaccg gccatccacc gttatttctc tatccgccac aaacggatgg gagacacaat
     1861 tcgcgcccga cacaacttcc tgcgtttatg cccggcatct aaagagcaag gcatgctcga
     1921 actggttaat gccattgcca acggtgccgg tcgatgtgat gcccgggaac tgaaccgtat
     1981 caaccgttcc acttatatca agcgtgtttg ggtcggatca agacggcatg gagactggca
     2041 agaggtcaaa gtcccctata tccgtaacgc caaaccttcg tactgctctg cctactttga
     2101 aaacggctgg acacagcttg gcacacgcta tgtaggtgaa gagaaaaatg gcgggaaatg
     2161 ggttgactaa tggtttatgt ggccgacctg cccccattta tgcaggaacg tgttacctgc
     2221 tcgattgctg cggctgaaaa ataccatctg ccgccacatt acctgttggc cgtggccgaa
     2281 cgcgaaaacg gtcgtcccgg tcaacaggta cgcaatacca acggcaccta tgatgtaggc
     2341 gcgatgcaga ttaataccgc ctatatccgc accctatccc gttacggtgt tactcagtca
     2401 gccgttgccc aacccggatg ctatgcctat tacttggccg catggcggat caagcggcac
     2461 atcgtttatg atagaaaggg agatatttgg acaaagatcg caaactacca ttcctatacg
     2521 cctaaataca acgcaatcta tcgcgcagcc atcattcggt tggccgaaaa atgggaacgg
     2581 tggcttgccg gtaggcaagg gaataacgcc aagggaaatg ttcccacaca agctgttcca
     2641 gccgttcgga caattcggtc gcaaccattg cccaagcaat atgtttcccc gacgaccgtc
     2701 tatgttccta gaacaatccg tgtggtagag cgttccccgg agccagagcc ggaaaccgtc
     2761 caaaactaac agtaaagacc atctgaaaaa gagtttcaga cggtctttta taatttcccc
     2821 ttgacatgga tggtaaattc atgtttatat caattcaatt atttaggagg tatggaaatg
     2881 gcttacgcaa ttgcatttga tttggatacg accgttttac aagaacttta ccctaatagt
     2941 agctggcaaa atgcttacgg tgatgtaaaa agaaccttgg tcggattagg ttttaaccat
     3001 cagcaaggca gtgtatattt tggaaactct gagattacag ccgttagctg tgtcttggcc
     3061 gcccaaaaac tgagccagac ctatgcatgg tttaagccgg ccgtttcgga tattcgtatg
     3121 ttgcggattg aggaaatgaa cgatttgcag cccgctttat aaagcgatag gtatcttaga
     3181 aaagggcagc gcggtgtccc cgccctgccc tttccctttc cttccagcgt tgccgagtaa
     3241 aggctgccta gtttctacta ggcagcccaa actactttgc cttgcaagcc cgtttaaccg
     3301 tactttcgct aacattgaaa tgtgcggccg tccgggaaat actggtttgt tcagactgcc
     3361 gccatcggcg gatttcttcg ggatcgactt tcactttccg ccccagtttt ttcccttctt
     3421 ccctagcatt gcggataccg gccatttgac gctctttgat gtaactgcgt tccaactccg
     3481 caaatgcgct gacaatagcc agcatcgccg tacctaacgg actggccaaa tcgaagtttt
     3541 cccggagcga ggttacggcc acgccctttt tctgcaaaaa ctgcaccgtc tccaaaacat
     3601 cgatggtatt tctgcccaaa cggtcaatgg ctactacgac tacggtatcc ccttggcgta
     3661 caaattcagc catcttacgg aactcaggcc ggttaagggc ttttactgca cccgaagcgg
     3721 cctgatcttc ataccaccgt tcgatctggt atttgtcttg aataacctgt ctttgtgcgg
     3781 ccacattctg ctctttggtc gataccctca aatagccgat aatcataatt cctcaaacgg
     3841 attaaaaact cttgcgccca agtgtgaaaa atccttttca ttgcgcgtta ccaccatcat
     3901 atgctcctgt ccaacggtcg tcttcgataa gcacacggtc aaactcaatg tctgcaatat
     3961 ctacctcttc cggaggaacg gccaaggcat cagcgatact ctccccctta cccaacgatt
     4021 ctgctcctaa cgaaaggtca tggggacagc cattcaaaaa cggagacaac cgtaccttat
     4081 aagccggaaa atcggtaaca aactttctca gtttttcatc aaccatcctt tgaacaaatg
     4141 gattgcctgt cgctgcctcg atgttgtgca aaatgtcggc caaatctccg attatcgtca
     4201 aattatccag cgcagccgca taactctcag gccgataccc gcgccgggaa taagacagtt
     4261 ccctgataat cagcgaaatt tccgtgatca accgcatttt ttctttcatt tccgtatccc
     4321 tgcttatctg ctgatggatt ttttggcgga atactccaaa gacttgacaa tcaatttgcc
     4381 cttattgctt ccgatagaaa cgttctgccc tatctcaacc aatttggcca gtctttcttc
     4441 gttatgtgtt gcattgtgcc tgacaagccc gtttttggtt ttttgaaata ccgcagcggc
     4501 atccttgtaa atgatttctc cggaataagt ttgaccagtc tctgcttttt tagcttgacc
     4561 tagtaaatag ataccggctt tcatgcctgt tacaggaatt tgttccacct tagaccacaa
     4621 ctcgtttgct gactttgttt ctagggtaac acccttggag ttagtaacta ccctttgctg
     4681 ggaatactct tttttggcga aaacactttc aagatccata ctgcccttgg cctgattgaa
     4741 gcattcccgg ctgatttttc ctgagtcgaa gtcgctctgg attttctcgg ccaacctgcc
     4801 caatatttgc tcttgcgacc ccaaggaaag cattttgatg acatcatcga acttgtcgat
     4861 aaattcactg ttcctgtctt ggttgatacc gacaattttt accgcatcac caaacttatc
     4921 cctgatttgc ttgagtccgt caggaagatt ggcctgaata tcagccataa tacctatact
     4981 ggctcccctg ccgtattcgt taaaccgttt gtaagtattg tcggaagccc tttcagccct
     5041 catgctgacg gctacgatag taacttcaag attacgctcc agacactgct caatcttgcg
     5101 aaaagcagac tgcggccgtg caagctgtcc ttcaaaaagc agcttggtgg tatcctgcat
     5161 catcatgttt tttactgtcg aagtttttcc cgaacccgga attcccgtaa cgaaaataac
     5221 ttctttcttc tgtggctcgt cgcgtttcag tacctcgtca aactgggtag aagataaaac
     5281 tgctgctgaa ttgtggatgg cattattaac cgttgccctg tcttctttgt tctcaaaagc
     5341 tggaaacaac tctttaaacg tatcagcaca aacatatcta ccgccttctg tatctgcatg
     5401 ttggctatat aaagacaata tctcgtctgt ttgttcggtc gcaaaacgga tggcctcatc
     5461 ctgaacggaa gacaaatatt cgttattgcc aaaatctctc aaattaatat cgctagacag
     5521 ttttaccata gcggattatt gctccttatt tgccgcccac aacactctca acttagctag
     5581 aagctccgga gatgggccgg catacaagcg tttttccact tctttgcgga aggcttcgtc
     5641 tgtcatcatc ctgcggtttt tttcgcaagc ctcttttatc attctgatag cattactttc
     5701 ttggggctca actttattca taataaaatc ctaatcttaa ttttatatta ctcatcatcg
     5761 cttcatcgtt ttctttaatg aggcatctgc cggtttaacg gacaattcat ttccttcccg
     5821 cttaatagaa accttctgac caatttccac ctgttcggac aaactagaaa actttccttc
     5881 cgccataccc tgaaccgcct tatggcgtat cagaccttgg tcagtcttct ggaaaaccga
     5941 tgcctcgtct ttatggacaa tctctccgct gtattccctg ccggatttag ccggtttcgc
     6001 cgtgccaagc atgtgaatac cggctttcat ccccttggct tcagccttct caatatcctg
     6061 ccatgtgtcg cctgatttgc gttgcagtgt tgccccttct gaattagcca ccaccctttc
     6121 ctgacggtaa atctcttcca cacgctgata aattgcttca ctatgctgtt tgaccatgcc
     6181 gagataatta ggtattggat cgatactgcg gctttctgcc tgaagctgta tctcgtccca
     6241 tgcttttaca acctgtttca accgtttagg ggttatttcc tgataagccg atagtgcatc
     6301 ttttgccgtt tcatgctggt tttgtcgccg attcaaactg tcataaagga tgtctcctgc
     6361 gcggttggta acacgaaccc tgtcaaaact tgattcacat tgagacaacg tatccttgaa
     6421 tccagccatt gcctcgttat ggtacgactc ttcaacaaat ctaggctgtg aaacaccttt
     6481 ggcatgttgt tcctcataac gcttaaaaat cccggctaca ctctcttcag aagcagtggc
     6541 taccgccagc atttctaccc cgtacccttc tgattttcta tcacggataa attggctgat
     6601 acttgcagca tttctaaatg tcccctcctc cacaatattc cgccggtttt cttttgcact
     6661 attccgaaca ctaatggcca aagcaccggc atctttctga gtttgttcag acgaatacac
     6721 caccccctcc ggcgcaggaa tcagtgcacg cataatatct gcatccacat ggatatatcc
     6781 gccttcttgg cgtaactcgc ttttagctaa agaagctgcc ttactcttac ccgctccggg
     6841 ttgtgcccca actagaacaa ttttgggttg ctccacactt tgcgttctag ataaacgtgg
     6901 cttcatgaaa ccattcagaa ttcttgcctt accttcggca cttaaagggg tgtactcaat
     6961 cattttacac gtccaactgt ttggtcatcc ggattgaaaa tacgcgcgat aatatctgtg
     7021 tcctccggag agagactgtc acgcaacata tcgatagcct caagccttgc tgtacagtat
     7081 tcgataaacg cttggtttgg tttatccttt acaaactcgg actctcttgc ctgaattaat
     7141 gggcgcatcg aatctcccaa caactccaaa gccacttcat aattgatacc aggaatagtt
     7201 tcttgcattt gcatcttggc ttcctccaaa agttatccac aaattttgtt agtaagctgc
     7261 ccatatcgcc agcctgccac atataataaa ttgaatcttc gtcatcaaac cgtttttgcc
     7321 tgctttttaa gcaacatggt tcaaaaccta tgtttgaata ataagccagt tcaaaataaa
     7381 taacaacgtt tttgacctat acttccagac cttaacgaaa catggttcat aaagtacact
     7441 ttttgcacct acttatctaa gttacccacc tcttaatcaa gatttggcca ttgcccacca
     7501 tatcccataa tgtgtagaaa ccagtagcgt tgggtggatg acggcgtttc gggttttccg
     7561 caggcggcag gcagcctgca atggtagtgg ctaatcctgc gcgctattgc atgagacaca
     7621 aacttcaaga tagcgcagca aggctttttc tatcgtttgt cgattaacat caaggtagaa
     7681 gagagactta tattcggatg caatgccttt tgctaggttg gcaataaaca tcttgtcgtc
     7741 ttcatctata tcaatatggc tgataaaagt cggtttggcc aaattcttga cagtaagata
     7801 ggcattgaat tctccaacat tcatatccga gaaatagtat tgcagctcta tcggtttcat
     7861 tttttcttcg attttggctt caatattctt caccgattcc cgtcgttgtg cttctttcag
     7921 gcagcctgaa aagttgtttt tcagtgccaa tgcgtgcagc agagcgtaca gggcatcgtt
     7981 accggctgcc gacagcggag gcacggattc gagcagtccg ccggagacgg tttcggctgc
     8041 cgcaccgtag cggccgttct ctttcgtcag gcggataatg atttcgtatt cttggacggt
     8101 tttcatgttc atactcccag ttgtctttgt tcagtccgtg ctaacggcgt atccagccgc
     8161 actccggcgg cggcatcctg cccgactctc tttcacgttt ataattatag gataatttat
     8221 cctacttgtc aaataaattt tagccgctaa atttttactc tcgcttattt attcattata
     8281 ataaattaat cgttattttg tttaactgta aaattggaga ttccttgaca aatatgctct
     8341 tacgtgctat tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc
     8401 ttaaataaac tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg
     8461 cttagttttt tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag
     8521 aatatctgta tgctttgtat acctatggtt atgcataaaa atcccagtga taaaagtatt
     8581 tatcactggg atttttatgc ccttttgggt ttttgaatgg aggaaaatca catgaaaatt
     8641 attaatattg gagttttagc tcatgttgat gcaggaaaaa ctaccttaac agaaagctta
     8701 ttatataaca gtggagcgat tacagaatta ggaagcgtgg acaaaggtac aacgaggacg
     8761 gataatacgc ttttagaacg tcagagagga attacaattc agacaggaat aacctctttt
     8821 cagtgggaaa atacgaaggt gaacatcata gacacgccag gacatatgga tttcttagca
     8881 gaagtatatc gttcattatc agttttagat ggggcaattc tactgatttc tgcaaaagat
     8941 ggcgtacaag cacaaactcg tatattattt catgcactta ggaaaatggg gattcccaca
     9001 atctttttta tcaataagat tgaccaaaat ggaattgatt tatcaacggt ttatcaggat
     9061 attaaagaga aactttctgc cgaaattgta atcaaacaga aggtagaact gtatcctaat
     9121 atgtgtgtga cgaactttac cgaatctgaa caatgggata tggtaataga aggaaatgat
     9181 taccttttgg agaaatatac gtctgggaaa ttattggaag cattagaact cgaacaagag
     9241 gaaagcataa gatttcataa ttgttccctg ttccctgttt atcacggaag tgcaaaaaac
     9301 aatataggga ttgataacct tatagaagtg attacgaata aattttattc atcaacacat
     9361 cgaggtcagt ctgaactttg cggaaaagtt ttcaaaattg agtattcgga aaaaagacag
     9421 cgtcttgcat atatacgtct ttatagtggc gtactgcatt tgcgagatcc ggttagaata
     9481 tcggaaaagg aaaaaataaa aattacagaa atgtatactt caataaatgg tgaattatgt
     9541 aaaatcgata aggcttattc cggggaaatt gttattttgc agaatgagtt tttgaagtta
     9601 aatagtgttc ttggagatac aaagctattg ccacagagag agagaattga aaatcccctc
     9661 cctctgctgc aaacgactgt tgaaccgagc aaacctcaac aaagggaaat gttacttgat
     9721 gcacttttag aaatctccga cagtgacccg cttctgcgat attatgtgga ttctgcgaca
     9781 catgaaatca tactttcttt cttagggaaa gtacaaatgg aagtgacttg tgctctgctg
     9841 caagaaaagt atcatgtgga gatagaaata aaagagccta cagtcattta tatggaaaga
     9901 ccgttaaaaa aagcagagta taccattcac atcgaagttc caccgaatcc tttctgggct
     9961 tccattggtc tatctgtagc accgcttcca ttagggagcg gagtacagta tgagagctcg
    10021 gtttctcttg gatacttaaa tcaatcgttt caaaatgcag ttatggaggg gatacgctat
    10081 ggctgtgaac aaggattgta tggttggaat gtgacggact gtaaaatctg ttttaagtat
    10141 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
    10201 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
    10261 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
    10321 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
    10381 cggtgtattc aaaaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
    10441 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
    10501 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
    10561 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaaaatta
    10621 actgtgagtg attggagaca ccatgccaca tgagcaaatg attgatgctt tttcagcaat
    10681 cattcaaaac tccttaagcc tatatgaaga gcaaactccg ctatactact acctgactat
    10741 ctgcggttct tgggttctga ctgcactcgg catcctctcc ttggcgtccg tattctttac
    10801 catcaagcag aaaaaaccat accgttttat cgtcccgatt ctgtttttat ggctgggata
    10861 tgaaagcatc cacatgccgc gcccgaatct tgctgtattc tacggataca acttggcgca
    10921 gctttcctat ccggcaaacg aaaatcccaa ctcgttccta catctcgcag acggctcaaa
    10981 tccgatttgc tgcttcatag gcgaagacga gtatttgcaa gccaaacggt taggtttcaa
    11041 agagtatgcc gatgcaaaag caaaggcttt ggttgatttg gttgaaatgg agaaaaaatc
    11101 caaacaatct gattgaatct taaattcaat aataacgcgg tagggggctg gtcaagcgaa
    11161 ggttcaggct ctgactgaaa tttcgcttgg acagggggtg tggggcggag cgcagcgaag
    11221 ggctaccgcg tggcggccga agccgcccga caaactccag tttgtcgcta ttccgttttc
    11281 agtgcgtaaa tacctttccc cactctcaga aaaaaccgct cgtcctgtaa tacctgcctg
    11341 actttggcct gccagttcct gttttctgcc cgcgcagcgg catacggttc catttcgcca
    11401 taaatttccg tcaagcccat attcctcccg tctgccgcct gcaatacccg tcttacggcg
    11461 gctttccatg taacggccgt taccgcctca accctattca tcgctttagc caaaaagtac
    11521 aaggccgtct gaatctcttt tttaaagacc agcagtttct catgggcaat ccgtaccagc
    11581 tttccgccat attggcggct gtcgctgacg cagttgtgct ggattttgat aatctcatcc
    11641 accagccgcc ccgccgccat ccgttcaacc atgctgctca aattgtagta acggccgtcc
    11701 ttcctcatgt tgcccattag aatcccataa tgcccgccct gttcggtcgc atcatggatg
    11761 ttcatcatgg ccagcagcat agcctcttga aactgttcca agtccatttg gctcaaatcc
    11821 cacggattgg ctgcatcacc ccattggttg ccactgtatc gaatcatgtt ccaataaggc
    11881 ggatgccacc aaattaaatt ggcctgttct cctaaaaatg ccgcaaaatc ctgagtcagc
    11941 aggttatatc cctgatgcaa atccgttcct ttaaaacgaa tgcccatttc ctccgcaaca
    12001 tccgaactgg taccgctgcc gatgctcggg tcggcaaaca gtccttccgg agcaagatag
    12061 ctttccacaa agtcgcggat gatatgcccc gtgcaatttc cgcgataacc gcttttaccg
    12121 taatttccgc gctgcggata agaacaaaca gaatgcgtaa acatggttca ttcctccttt
    12181 aaatcgtctt cattttcgcg ataatggcaa cctaatactg catccaaatc ccgcatggtt
    12241 tccaccggct cgtcattgac ataaaagtgt gtatccagcg cattgattaa aaaacccgtc
    12301 cgcaagaacg gccaccggtt gcggatggta tcctgcaaac gctccagcaa atgatgctgc
    12361 cgaaggtttc caaaatgcgg aacggtttca tatgtccgtg ccgtttccca aatgtcctgc
    12421 tcgaagttga taaacgagtc gccgccgagt tggcgcagca gattcatcca accatcgaaa
    12481 ctgtcggaca catcatccca ataaagcata atttcctcct tatcactcaa tccctgcatc
    12541 ggccaaaatc aaagccaaga tgccgctgcg ccgtgctttg gcagttttca gtttcagaca
    12601 ctgcaaatca tcgcaaacaa tctgataccc gcgcgtctcc agttcggccg cttctgacac
    12661 ttgcaggctg taatactgcc cgccatcgaa tgtgtatcgg caaagcagtg taggggcatt
    12721 tcgtccgtcc cacataacgt taatcggact gaatcccatc aactccccat tgctccaaat
    12781 attctgctcg acaaatacat tcggatgccc agttaatctg ttggtatagc ttgccatctc
    12841 tgtctcccgt actttccttg gtgttaaacg gtttttgaac tgaaccagca tcggcgtttc
    12901 cgccccgctg tgatgacgtt aaaagacaaa ctgctttgag atacaaggct gccgcaggca
    12961 ccgcaggctc ggccttgtat ctcaaagcag tttgtcttag gaatcacgca acagcggggc
    13021 gggaatgccg gttgcgtcga acggccaaaa gccgtttgac accaaggaaa accttctgcc
    13081 ggccgcagcc ggctcattgc ccctcacggg gaaggggtgg ggttcggaga ggcggtgccg
    13141 cagcacggcc gatacttaaa ccaagagcgg tttgatgcct tagcatgaaa ccgctctttc
    13201 cgttcaatcc tgctgactct tgattagcct gaaactccca gcaccttcaa gataaccgtt
    13261 tatagtcttg cgaatggtaa catattccgt ccccatcatt ccggatccgc ccttagtcca
    13321 aaaaaacggc tccctcggca aaaaaggaat atattcctcc tcttgaggct cgcccatgcc
    13381 aagactgatc tcgggctgat tcccatccgt ctcataatgc agcttttcgt cccctttgaa
    13441 aaaatcggcg acgaccgctt ccgcctcttc aagagattgg ctgataaata aagccgaact
    13501 accaaagccg atctcacctg tctctacaag gtagaaagtt cgattttcag cggacagact
    13561 cattttctcc actcccgata aacgcctatg tcgttatgga taaaggttgg atagataccg
    13621 atcccaccta ccctgaacct tcttgccaaa gcggcaagct cggaaaggga cgctctttcg
    13681 atatgaaagt cccccgcatt gccggacaaa tgttgggagt ttttggccgc cccctctaac
    13741 gagtcgttat gtcggcgcgt gcggaatccg ctatgcaaaa ccaactccgg attcggtctg
    13801 cccaaatcac gcaaatactg ctgctgggca aacagcagat tgatcaaatt gatgttgatg
    13861 gcgtgcatct gatggttgtc caccacatcc cgcatcatcc aacacagagc cttatagccc
    13921 tcttggatat actggccgtc ggcaaagaat ttgatgtttt tccgttcccg ggaaacaggc
    13981 cggtaaatgg acaatacccg gtttcgtttc caaaaccggc tgtcatccgc caaaacgatt
    14041 tccggcaaca gtaaaaggcc gcctgatgaa gcaagcgcgg ccgctttggc cgacagcttt
    14101 aaaaaatccc gacgattcaa cgctccctcc ctttcacgtt accgctgacc ataaaagcat
    14161 accggccgta ttcgttacct ccctcattcg ccgtttctcc ggccgcaagg gatcccaaaa
    14221 cctgctctaa tcccatttcg accgcaggta taaaatcttc atctgtcgcc gtaatggtaa
    14281 tgctgagcgt cttggtatcg gcatgcgtat ctacccgatg attcagtccg tactgcttct
    14341 gaaatgcctt gacggcggcc tgttcggact gattccgttc ggccgacacg cccatttttt
    14401 cctcttcgtg ccgctgcaac atttccaaat aatcgtcaaa cgccaagggc tggttctggc
    14461 tggttttccg gtaatcgacg gcgatatttt gcagcgtctg ccgcaggcgg ccgtcaaagg
    14521 ccatgccgaa aacccgccag aaccatcctt ccctggcctg tttgtgataa gtcagaaaaa
    14581 aagccactct gtataatttg tccttatcca taacaccccc tatcggctgc cgcgcgatgc
    14641 ggttttccgg gttgttgccc gctgctgccg cttggtctgt tcgacaggcc gatcctgaga
    14701 cgcttcttcc tgcaaaccgc gtgttttcag ggtttgcaat tcgtttgccg gtcgggcatg
    14761 tatcgcttcg ttgatttgct tggccaaacc ttcgcggccg aacttggatt tctgagccaa
    14821 atcgttgaaa tcggtaaatg ccttatgctc cgacgactgc tctcccggtg caaacactgg
    14881 aaacactgca cggcagttaa ggatttttgc cgcctccaac gctttttctt tgccgggatt
    14941 gttgccctgc ttggcttcga cagccaaatc atcgtctccg gcgaccaaaa actgagcctg
    15001 agggtatttc gcagacaaga ctttggccac caaaggcagg ttgccggaat tgaacgcggc
    15061 cacactggcc accgtatcgt cggttgcttc tttaatcgag gcggccgtag catagccttc
    15121 cgataaaata atggttttgg ccttagccag atcctgttgg ccgacaacgt gcatacaacc
    15181 ctcctgctcg ctgtctttgg caaaacgttt ggtaccgtcc gcctgaatgt attgcatgga
    15241 gcgtaactcc ccggctgcgt tgtacacggg aatgaaagtt ttctgcccct cgcggtcggt
    15301 aaagataccc ggctgcggct tgataccctt ggcattcaga taaggcgtgc ctgtttgaac
    15361 gggcaggtag ctttccatat agtatttgat acggccgaca gcagcttcct gagcctgttt
    15421 tttctgctcg ctccgcattt gcagcttctg agcggcctcg gcggccagct tggctttttc
    15481 ctccggagac aggctgtatc cctttgcctt ccacttaacc tccgctcccg tccggttgtt
    15541 tttgatgtag cctgccgggt ggccgtccaa atgaccaaca taaaagccgc tgcgctcgcc
    15601 ttttttgtcg ccgacggttt cgattcggtg tttttgtccg tccataaacg gatgcccatt
    15661 agccaccaca caccccattt cgcgcaactt gtcggcaaac tcttccaccg gcgacatcgc
    15721 gggcatgctt tgagtctgtt gcttttccgg caaccatttt gacgcggccg gtaaattagc
    15781 gttactgctt tccataaacc aagatttgtt ggctttgtcc catttggcac caagggcttt
    15841 agcctcttct ttttcttcat acggaacggc aagataaaca cgggatgcgt tttcttctgc
    15901 cggtgcttct tctgttttgg cagcctgata ctcttgctgt atggctgcga cctgacactc
    15961 aactgcttct tcctgaggag tatcgtccgg cacggcctct tctgccaacg cttcctgctt
    16021 cggctgcatc caccgtgcaa acagtgcggt attcacgcct tcggggatat accaagactt
    16081 ctgatcctta tcccatttcg cgcccaatgc ccgcgcttcg tttttttcac gaaacggcac
    16141 atcaattagc gttttcgcta tggccggagt attgtccgtt accgtttttg cttggttggt
    16201 ggcactgtcc agccattttc tgaaaggctc ttgattgacc ccgggaggga tgtaccacga
    16261 tttctcctcc ttatcccatt tcgcgcccag catttttgcc tgttccctgt cggcataagg
    16321 aatttgaacg taaacgcgct catcggaaac cacgcggacg ctttccttgt cctgctccgg
    16381 cagaaactgg cggatctgtt cttcaaccgt acgataagag gactgctcga tggcttcgtc
    16441 caaagcctct ttggccgcat tcagattttc cataaatgac tctccattct ccggtttttg
    16501 atgccgctgc caaaaagcgt tctgaaacgt ggcatcatcc atctccgaaa taaccagact
    16561 gaaacgcttc aagccctcgt gcattttctc ttcagccatg aagttgatga tcgccgccac
    16621 atcattttta tcaaccgcat tgataaggtt cttttcttcc gccaagctgg cttcatcaat
    16681 attctgtcta tcagaaatac tcactgccgc ctcgttctgc tgttcgattt cccgcacctg
    16741 atcaaaggcc atgacatagc cttggatttt ttcggcgtca gaagccgcac gcatgatttc
    16801 catcggatcg tcctgcaata ccataatcca gcttgctaca taactggcat gttgcccggg
    16861 attgaatgaa acgcccaatt cctgactcag catcaatgaa gcgatttcgg cacgcaattc
    16921 ctcccgtgcg taaccctcgc tgccgaacgg gtgggaaagg tcgcggttca aacggtcagc
    16981 atgccccgtc caatgcccca attcatgcag ggctgtttca taatacgctc ccggactttc
    17041 aaactgctcg cgcagcggca gggtaatact gtccctgctc ggagagtaaa acgccctgtc
    17101 gccgtgccgg tggtcgatga ccgcaccaga atttttcaga atgttttctg cccgctcatc
    17161 cggattccaa cttagcggcc gcagcttcaa cggcggcaag ccgtctatct gttcgccgtt
    17221 aaataccacc gcatgaaaga cctttggacg ctccaaacga accgtttcct tctccggttt
    17281 gccgtcctgg ccgataattt cctgaccctt ttcatcggtt ttgggaactt cctcggtgaa
    17341 ctgccaatat tcgacaacag tacccttctc tccacggcgc acctgcgccc cgatggatga
    17401 tgcctgttta taggttatcc agcgcggatc ctttctgcct tgtgcctgca accacagatt
    17461 gttaatgccg cgataacggc ggccggtaat ggcattgtgc ggcataaaat ccggcaaccc
    17521 gccgccgtcc caaggtttct gccacggcgc ggttccctgc cgcaactgtt cgatcagttt
    17581 ttccgccacc tgttcgtaaa acggtttttt attctgcatc cctacgctcc ttccgccact
    17641 tcgcgggaaa ggtctgccac ggaatcccgc gcggtttccg catccattgc ggtctcctca
    17701 aacgccccca aggcttctgc ctctgccgga tcaaaatcca cttccattcc cgggttattc
    17761 aaatccgcca ctttttctgc gatcacttcg atgtcgatac tcatacctta tcctttcgta
    17821 tgtttggaat agtccggctt accgccagca tcggggtaat actgcttgca ctcggggtaa
    17881 ttgctgcatc cccaaaaatg cgttcctttc tgttttcccg gccgtctgac caagggatgg
    17941 gtgcaggcca tgcacttgaa ttttgacgac ggctcggctt ttttcggtgc cagtaccggc
    18001 cgaccggcct tgtcggaaaa agaaaccttg catccgtcct tatagccggt acagccccaa
    18061 aaaaatcctt tcgatccgcg catccgcctt aaaggtttgc cacattcggg acacgggtac
    18121 ttcttgacat tgagactgat gccttcccgc cctacccgct ccacctcgcc ggatatgtac
    18181 tccatcagac tgttcacaaa acgttgcgcg tccaaaccgc cttggacaat atgctgttgc
    18241 tgctcatgcc ataaggctgt catatcgggg aacttcgcct gatcgggcag cgcgtcataa
    18301 agctgttggc cgatttcgga actgacaatg tttttgcctt tttccagcag aaaacccttg
    18361 tccagcagat tacgcagaat ttcgtcccga gtggccgggg tgccgattcc gccgtgctct
    18421 ccttccttac ccttgtcttt ctcaatcagc agttcgcgca atttcggatt ctgcacatac
    18481 ttggctaccc gtgttaaatc gttcaacagc gtatccatcg tgtataacgg gcgcggtttg
    18541 gtttcaaact gcgtctcttc tgctgtcaaa caccggccgc tgtttcccgg tttgagcatc
    18601 tgcaaatcaa cactcaaatc ctcgggatct gtcgcggttt ccgcattgcc ctgatcgttc
    18661 ttgtatagag ccatccaacc gggctttacg gttacgttgc tgcggcagac aaatttttta
    18721 ccggcaattt ctgcttcaat gtcggtttgg ttatattcgt gatccggcca aaactgggcg
    18781 atataggcgc gggcaatcag cagatagacc cgtttttcgt cttcggtcag gtcgtccata
    18841 ttggccgttt tgacggtagg aataatggca tggtgcgccg aaactttatc cgggttgaac
    18901 gccctgcttt tgattttcgt gtcggccgat gcaatggcat ttttaaagac tgatgcggtt
    18961 tttccgacag catccagcac gtctgccgca tcatcgaaat gctcgtcatt gagatactgg
    19021 cagtcgctgc ggtggtaggt aatcaaacgg tgtttgtctt tcaatgactg tccgatgtct
    19081 tttacctgat cgggtttcaa accgaatttg cgcgaacaat ccgcctgtag tttgagcaga
    19141 ttgtatggca gcggcggact ttgccgcttg gcagaagttt tagccgctaa aatttttacc
    19201 ggctgaccac tggctgcttc ggctatctgc cgtgcaaaca gcggatcgga caaacgtccc
    19261 ttatcgtcta tcggatcttg atccttgttt ggaacgtaac gggcagtaaa ccggacacca
    19321 tccacatcca aatcggcata cacatggtaa taatgggttt tgacatggga ctggttttcc
    19381 cgatcgcgcc gcaccactaa acccaaaatc ggagtctgta ccctgccgac ggacaacact
    19441 ccttgataac cctgttccgc agccttcagc gtataggctc gggtcatgtt gtatccgtat
    19501 aactggtctg ccaccgatcg cgcttccgcc gattgggaca atcccctgaa tttggcgttg
    19561 tcttctattg ccgataacgc tctctttaca gatcgctcgt tattgtcgtt aatcagtacc
    19621 cgtttgaccg gtttggcaca attcaaaaac tccaacagtt cgtcaaccaa caactgtcct
    19681 tcgtcatcgg gatctccggc gtgaacaatc tcggtacatt ccgccagcaa atcacgaata
    19741 accccgatct gttctttttt gtcggacaag ggttttttac gccaagggat atgcgcgatc
    19801 ggtaaatcct caaacttcca gttaccgtat ttgctgtcgt aatcttccgg atcgcacaaa
    19861 gcaagcatat ggccgtaaca ccaagtgatg ttgtctccgt tactgcaacg gaaatacccg
    19921 ttgcgcctct cgataccaac tcccaaagcg gcggcaatgg ctctggccac ttcgggtttt
    19981 tctgcaatat aaagtcggga catacctact cctctatcca tatcggtttt acgacatcac
    20041 ggatatgctc tctccggact atgccgtaat aacgcgcgtc aaacgaattg gccaccgcat
    20101 taccaatcaa aatgtactca tcgtctttca gacggccttc cccttcaaat accggtaagg
    20161 gctgccctag cccgtcttgc cataaaggcc gggtgccgcc aagcagccgt ccgttaatca
    20221 acaacccttc atcggtaaaa cggtaattgt cgccgggaat accggcaaca aacttcatca
    20281 acccggaata attttccgga caaaaacccg ctctgatata ccccctcctt cttgccattc
    20341 ggaaaacttc ggtatcggga gggcaaaatg taatgtagct tccgcgccgt accgcttcgc
    20401 cggatatgcg gtaaacccct ttggccacac tgcgggtatc gttgatacgg aaaccggctg
    20461 catataacag cccgtagcct gttaccagca atgcggcggt cagacacatg gcaagcatgg
    20521 cacggtcaaa cagccttctc atagagtgtc aaacgcgctg ggcgtattgg cagtcttttt
    20581 ctgagtccgg tcggtatggc gcggaggcgg gactttggag cgcgcctcaa ataccggatc
    20641 tttgaaatac agggtctgcc gcccgtaaat cgccggatac ccggcaacat aaatgaccat
    20701 atcgcctgcc tgagtgatca aaccccttgc gtccttgacc ggcccgggca tacgcataca
    20761 ttcgtcggct gtcagcaacg gccgctggac ttcctgcgta gtccgggaaa cctgccccaa
    20821 catcgcactt acccgtttgc cgctggttgt gatctgctct ttgacaaccg ttgtgatgcc
    20881 ggtcagcttg gacaggtgtt ccgccgtctc gatccggtta ggcggataag cgttctgcac
    20941 atggcagctg gacgtaatgg tttcatccgg gccatacccc gtttcccggc ttttcagttg
    21001 gttcaagtct tggcagatca aataacattt gatgccgtaa ccggcgataa acgccaagga
    21061 ttcctgcatg atttccaact tgcccaaact cgggaactcg tccagcatgg ccagcatctt
    21121 gtgtttgtag tggactttcg gcctcccgtt ttcaaaatcc atcttgtcgg ccgacagacg
    21181 gataatcata ttgatcagaa tccgaaccaa aggccgcaac cgcgccttat cgttgggctg
    21241 ggtaatgatg tacagactga ccggatcttc gtcgttcatc agatccttga ttttgaaatc
    21301 ggaacgactg acattggcgg cgaccacatt gtcgcggtac aaagcgagga aagatttggc
    21361 tgtggaaaga accgatccgg cctcctcttc cggtctgtcc agcatatcct gtccggcaga
    21421 agaaatgacc ggcagaggac ggccgtctgc atggggatac gtcgccattt ccacccataa
    21481 atcgcctacc ggtttctccg gatcagacag catgcggtca acgcttggta aggtggcaac
    21541 cccgccatca tccctagctt tgtaaagcgc atgcagaatg acgccggtca gcaaagcgaa
    21601 agctgttttg tcccagtggc tgttcagccc cttgccgtcc ggatcgacaa tcattgtggc
    21661 cagattctga acatcgccta tctcatactc cgtccccacc ctgatctcat ccaaaggatt
    21721 ccatgccgcg ctgccttgcg cggaagccgg ttcaaagcgg attaccttgt tgtgcgcgta
    21781 tttcttccgc cacccggccg tcaatgccca taattcgccc ttcaaatcgg taatgaacgt
    21841 actgtacggc catgacagca aagtcggcaa taccaaaccc aaacccttac cggatcgggt
    21901 tggggcataa gtcaggacat gctccggccc ggaatggcgc agataatgca gggtaccctt
    21961 tttatcgacc cagccaccga catatacacc gtcgctgtcc tctccttttt tggccagcag
    22021 accggccgcc tcgatgtctt tcctgtccgc ccaacgcgcc gacccgtgca gagactcatg
    22081 cgccttattg gtattcttcc gcacaatatt ccataccaac agccctatca tgccgacggc
    22141 taccaccatc aacatgatgc tgaatgccgt cataaactgc tgctcgtacc gtccgtgcca
    22201 tttaccgtac caaatcagaa tgctccacgg catatagaac ttgccgatgc cgtatcccaa
    22261 ctgggaatgg tattggaact gatgggcaaa atattgtgtg gctgccccca taccagccaa
    22321 cagcaccaat accagacaaa taaagtttac caaacggccg cttcctgtcg cttgggcttg
    22381 gttctgatgg ccgaccgcat tgttgcgttt gttcttgctg cgaaacatgt ttatcctttg
    22441 aaatttaacg atgccttccc ttaacaggtc tgatctgccc cgtcggagta actttcactt
    22501 cactgcccag tttcacgctc gaaaggcgtc tggacacata gggtgtaacg gacatgacca
    22561 gtatttcctc gacctctttc ggctggaata aagccagtgc ctgcccgtct atgttgcgga
    22621 tgcccctgaa caacatcaca ccggaatcac ttggattata cttcctatga ttcaatatat
    22681 cgaatttttt gtcaattttc tcctgccgct cggcaatata tttgtcgata ggtgccaatg
    22741 aatgagtaag tctctcgttt tcttgtgcag gctcaggatt tttcggaatc tctactaccg
    22801 cagcggatga aattttgatt tcagccaacc cttccatccc ccgcaaactg ccccgcctga
    22861 atcccggcgg tttttttccc ggtttggcaa ccaaaccgac aactgcctgc atctgcctga
    22921 tctcagctac tcctcctgtc cgatgtattg tccgtcgcac agcctgatag tttgcggatc
    22981 cttgctgctc caagtgacta gaaacatcac gcggcaatag cacttgagtt cgtccttgga
    23041 tgcgaaccag actgcgttcg ggcattcctc gcagactgtc gcttcctgcg gaagcagact
    23101 gccctcttcc tgctccaatg cctccagagt cggagagctt cgggattggg tatcctgata
    23161 tgcctgcatg gaagcctcga tttcggcttg cgtcataccc atgccatcga tagcgtctag
    23221 gctttcctcc tgcatatccc gcgcctcctc gttgctgccc tgcccgccca tctgcgcggc
    23281 cagttccatc agcttgtctg tatcgatcgg tacgttccct gacatattcg tattccttca
    23341 catactgttg ataaagatgg ttcagacggc cgtctgtaaa cgacacatcc aaaccctttg
    23401 cggcggcaat tctggccacc ttatcctgaa aatgccgcgt tccgccgata gtcaggactt
    23461 ttccgtatcg ccctattgcg atttccaacg cccttgatac cgccttatcg tcaggaatcg
    23521 cggccagttt gagcacactg cccgtatcac ggatggtcat gccgtcctcg gcataaataa
    23581 ccgtcccctt tttcgttacc gtatcaacct tgatcccggc aaatccgcca ctttctttgc
    23641 cgacaaagcg gttgcccttg taacctgcat ccttccccct ccttctcaac gcatctaacg
    23701 cccggacatc gccttcggca gcacggtgtt tcagccaatc tgcccaagca aaacccttgt
    23761 tctgctcaaa aaaaaccgca cgtcggcggg cataatcggt tcttaccgat ctgatttcat
    23821 ccttcatttg cctaaacagc atccgataca gaatgcgcct tgcaatacca ctgctgctat
    23881 gcctaatcag gctgcggcga aaagcgtatt tttctttgat gcggccgatc tccatatccc
    23941 ggttttgtgc caaatgcagg aaaaaatcct gccgcctgct tttctgaagc tctttttcct
    24001 ccttgtattg ccggaacaga tcgtccacgt tggctttgct caacggtttc tcacggtacg
    24061 ttttggatgg tttcgccttt actcctgttt tctgatcaaa tgcaccaagc cttttttcca
    24121 acgccgactt ggaaaactcc ctatctaccg aacttgcctt gacatgatgc tcgccgtcgc
    24181 tgataacaaa accgttaccc ctcagcttga tttccaatcc gtgatcactc agtacatggt
    24241 acagttgctc ccatgtctta gcctctttaa tctgctcacc gcactctctc ctgatccatc
    24301 ctatcaggct ctcgataccc gccacttcct ccatatcgat ttttttattg ccgcgcccgc
    24361 cacgctgcac cctctgcaaa ccgtactctt cttcaaactt cctgcactcc cgccctaaaa
    24421 ttttgaagtc gttatacggc tcgtggatgg tctttttttc tgggtgcact ttgttaatcg
    24481 ctacatgaaa atgcagattg tcggtatctc tgtgaacaac agagatccgc tgatgttcgg
    24541 cataccccaa agcctcaatc atgctctgct caatacggct caactgttcg tccgtcaaaa
    24601 attccccgcc cgcaagggat accatcagat gataggtttt atccgccgca ctgcgcttat
    24661 tctgatcctg aactgccaaa gtctcctgaa ccgcccacct caaatccgat gattcatagt
    24721 tatgaatctc cacctttccc actcggtttg cgacaccttg agaaccggcc agataggtaa
    24781 ctaagccttc aaaactgctc aggttggctt tacgcatcgg aatttttttt gcaatcatgt
    24841 tcagcctatt tttttagccg ctaaaattct gcggctccgc acaatctcca acatactttc
    24901 ctgcatttct ccaatttgct ccaacactcc gaagatcgcc ttagccaact gatcgtgatc
    24961 gaacatcatc agccgctcgt cgttggccaa ccacatcttg atcaagccgc ccaatcggcc
    25021 ttggtcggca ttgattttgg ccaaggcttt aaccgcttca ccgtcataaa cggatgcagg
    25081 cttgtatccc aagccaagta atttcaaata agccgattgg ctcaatccga cagatttagc
    25141 caaactctct atggttttcc attcttcttc cgaaacgtaa acgctgatgt attttggccg
    25201 ttttcgtgcg cctgccattc ttggagtcct taatttgtgc cgcaaggcac acaacagcca
    25261 acggtagttg gccagcctcg cagagcaaga taatccgttg agcctaccgg cgaataagga
    25321 ttgtgatgta catgtacatc acatatcttg ccccaattct ggggttcaat acgccaagga
    25381 ttatacgcca aataattcaa tatatcgaac atattttgat gacaaaccgc ctaaaaatgc
    25441 tttatcacaa acaaaaaatg atttactata tcgaattatt gtttttaaaa cgattttgct
    25501 tggcagtttt ggcaaggctt tgtcaaaaaa ttggcaaagc cctatcgaaa tgatccaaat
    25561 gggaaaaata gaaaaactgg ttttggccga acttgccgcc atgcagaaaa aaggctcctc
    25621 agccggaaac aaggcaaggt tcttggccat gcaggaagac attaagatac tgctggtgga
    25681 caggaatatc cccgttatca gtgtttggcg gattttgtcg gaaaaaaaat atgtcccata
    25741 ttcctatgag acatttaacc gctactgcaa gatgtatatc ttcaaatccg acactacggc
    25801 cgctgaccaa acagacaccg cccagtccac acaggaaaaa cctgattcca tccgcacgga
    25861 caaagaaccg ccgtctcccg cctccgaacc cattatcgcg ggaggcaaca ccaaaagctc
    25921 ttcaggcttt actttcaaca acaacccaga cggaaaggac ttattttaaa tggccattat
    25981 ccatatcagc ctccaagcaa aagggggcgt cggtaaaagt ttcttggctt tgatgctgtc
    26041 tatgttttac cgccatatcg aaagaccggt aaaaattttc gataccgatc cggccaacgc
    26101 aaccctgttc ggttttcccg cactggaggc ggaaaaattc gagatcatga aaaaggatga
    26161 tattgacaaa cggttgtttg accggctttt ctcacaaatc ctgtctgcaa ccgatgatgt
    26221 catcatcgat gttggcgcca atgcgtttat tccgctttgt tcgtatatca tttccaacaa
    26281 tctgattacc atgctgacgc aggaaggcca tcaggtcata atccacactt ccattgtcgg
    26341 cggccagaac ctgaagcata cggtcaacgg atttgatgcc ctatgctcgc aatttccgga
    26401 atcaaccggt ttcgtcattt ggcttaatcc gttttggggg gaaattgagt ttgaaggtaa
    26461 gtcgtttgag aaattgaaag tctatgagaa acacaaatcc cggatacgtt ccatcattaa
    26521 gattccggaa tgggacgctt ctctgcacgg ccgcgacctt gccgccatgc tggaaaagca
    26581 aaaaacattc gacgaagcct tagcgtccga agatttcagc atctttgaac gccaacgttt
    26641 gaacattatc aaaaacgaaa tgttccaaac gatagaaacc gcccaaatcg tctgagtaca
    26701 tcatggatga aaaagtaaaa gccatattag ccaccattgt tgaaaaaaac ggcatcgcct
    26761 tatctcccaa agacccgata atggttgtat tcactgcctt agaatatatg ctctccgaac
    26821 agcacgcaga gcaaaaggct ctaaccgtct tattcaaagc gcaggtagaa gaggccacgg
    26881 gaaactggat acagtccgtt caccaacaga ctgaggaact tgtcagccag caactcaata
    26941 cgaccaaaga acaggcaaag gaaatgtcca aattactgct tcatgagaca gacaggctgc
    27001 ttgagcagag aattactgat gccgtaaaag actttgaagg aaagctatac ggcatcaacg
    27061 acaacgccat gaatgcagta gaattgagaa taaaggaaac tcggaacacg gcaattctca
    27121 atctgacggc cgccctgatt gtcttgttag cagtcattat cttggcgttc aaatttacct
    27181 tctgacaaaa aacccggcaa tgccgggttt tttgtctcca ttctttacgc caagagaaaa
    27241 atcagcgtac tattttttga cctagggaca ggcttaatcc agcttgccgt ttctgttgtt
    27301 ctttttcttt gcgctgtttt tcatgcagat tgtgaaccct gacagccgtt tgaacatcat
    27361 caaatttttt tgccagatcc ggttcctcca tccttaactt cctagcagcc aactcctgta
    27421 ttttcggaac acccagagcc atcccgtcgc gaatctcgcg aacatcttcc aacctgtcat
    27481 gcagacggtt cagccttgat tgtgccactg caatgtttgc actccacgtc tgttttttgg
    27541 acggcatgct caacattccc ggccgggcgt tttgcaatgc ctgcaacttg gccatttgcc
    27601 cggaaatcaa ttgctccaat ttttcttcta tgcgctcaac ctgttctgtc ttttccaaga
    27661 cataagtatt taacacaccg gcgaaagttt cctcaataac ctgcctgctt tcaaacaact
    27721 cttcttgttc ggtttcagcc gcaacagctt tagctacaag ccctttatct tcattcatgg
    27781 tgttatccta aaattttagc ggctaaaatt tcatttagcg cgttaaagtt tttttctctc
    27841 cgatacccgc cttttctact gttgccgctt tgtccttgac actgattgag accacttctc
    27901 cgatcgacgg aacggaagaa aatagagaac acgggtattt gactatagac tgtcgtgttt
    27961 tctgataaac atagctgtca ttttgaaaaa cgatttgccc ttcgtacctg acagaatcct
    28021 gaggagattt ggccgtatgc agcaaataaa ttcccggctt catattggca ggagcaggct
    28081 taaccgcctg cgtaatccat ttgccgtctt tttctgtctc gaacaaatgc tgtttgttca
    28141 ataccagcac tctttgtttt tctgcttcca ttctgctaaa aattttcaaa aaagatgtga
    28201 ataattctaa cagaacagtc tttaatatac cccccccccc ccataaatat ttgattttaa
    28261 aatgatttaa tttaatgaat tctgatcaac ctgctgattc ggtattttgc acataccggc
    28321 agcagtaaaa ctgggcagcc cgttaaaacg ggggggtatt ataataaatt cccagtatta
    28381 tccttcaata actgcaacaa tcccggaaat ttctttacaa ggaacagatt ctgtttcgcc
    28441 ggactcctca aacttaatcc atacctcatc gccagacaat ccgcgcttgt tcaaaaccag
    28501 ccttgccagt tggccttgat acattacctg cacaatagct tttttcagtt tgtcaggatc
    28561 ttccggcttt ttctctttca cttttgcctt ggatggcttt tcatgatcgg catcagcaga
    28621 atttttagga tccgagttta aaaactcttt caaattcctt accgaactac gggtaatctc
    28681 gtcttcgtta gacagcaaat cttccaccgc ttccggttct tttttataca atgcgttcag
    28741 gtcattgatt accgtcaaat cggatacttt ccccgagttg aatgcgttgg ccagaggttc
    28801 gggtaagtcc agcaggtttg catgctgggt aacgaaagca ggggatttgc ccattttttc
    28861 ggcaatctgt ccctttttca tcccctctgc cagcaatcgg ccaattacgt cggcgatttc
    28921 ccttggtgtt agattatcgc gctggatatt ttcaacagcc tgatcgatca ggctgtaatc
    28981 ttgatcgata tatgcgggaa tctcgctcag ccctgccagc aatgaagccc tgtaacgtcg
    29041 tgcgccgtgg ttgatgacat agaccccttc ttcggaactt tgacgcaccg atatgggtgt
    29101 tttcacgcct ctctcctgaa tgctgtttgc tagttcctga atttttcctt cgtcaaattc
    29161 ttttcttggt tgatcggggt cttctttgat tgaagcaagg ggcaggcgca gcggcgtccc
    29221 tgatgagacc tctgcattac ccaaaagttc gctgagattg ccgatgtttt ccaaacccaa
    29281 attactgtta ttcataaatt ttacccttgc attttgttta aaacatagtt agaaacggct
    29341 ttcatttcct gcgccgcctt tcttgctgcc gttttcttga ttttccaaac cggcaggctg
    29401 gaagacaacg catctgcgat actgctcctc aatccgataa tatgcggtat tacaatttca
    29461 ttatactgca ttttaatttc ttccaaatgc cttttatggc gcggattcct catatcgacc
    29521 ttacttggca atatacccaa aaaagccaat ttgctgttca cttttctgat attgcctatt
    29581 gttgcagcca tctgtttaat tccttgtagg ctgtatgttt ccaactcgat cggacataac
    29641 acataatccc cggccgccag tgcagccgcc aacgtgttac ctaaactggg cggagtatca
    29701 ataatacaga catcgaattg cccgcccgct tttaatttgt cgatgttttg cgtaaaagac
    29761 tgaacagcat tttgcaaagt catattctgc acgttagcca aatcgtttgt cgccggggaa
    29821 agagccagtg aaacgttgcc gggcatatgt aaatccgcat cttccatatt tccaaacaaa
    29881 cggccggaac cgaaaagaca cgttttgtct cgaagagagt aactcgcatt cccctgcgta
    29941 tccaaatcaa ttactaaaac cctcagtcca actgtttcca tatgccaggc aagatgaagc
    30001 gacatagccg tttttcctac cccgcccttt tggtttgcca ccactaaaat cttcattcct
    30061 acacctcata ttttatttct gaacgcgata acaaaagtga gccatcccat tacgataccg
    30121 aacgtcaaga taaatttgcc gatataaatc catgaatcag ccatattcca gtggtacagc
    30181 atttggcaca aaacatagaa actgtacatc caagcaaacc atcggacagg gaaaagcaaa
    30241 taggcgatag ctaacaccat ataaaccact tcaaacattt ttttttccag ctttggattt
    30301 aattctgtcc ataggagcct ccgtccgata tagtttgtat tataggataa attatcctat
    30361 ttgtcaagca attttagcgg ctaaaatttt atttaccaaa atttctcaat acccacatac
    30421 aagctcaata aaacccaata ggcatatgtg atggatgatt ccccactgat gaatctcctg
    30481 aaagctctac cattggtgtc tttcaaccct aaatacctgc taacttgttt ttgagtcatt
    30541 cccgttgcta accacaattc tttgacctca tcagccgtag ggactgccca ttgcggaaac
    30601 cggtggaaac attctggacg gatattcagg tctttttttt gtatcaagtt atgctcttca
    30661 atattttgaa gatgccattt ctttgcttga aatgcttggt aaggagtaag taatacggtg
    30721 gtagaaatta aatcggtcat ttgaaagttc tcggcaattt acccttggag taaaatgaaa
    30781 aagatctaaa tcgcccgtag ggcgacagac gaatttatga aattatctaa aatcgccgta
    30841 ggcgacagca aattgataaa accggctttt cggtcgcgaa atatggttat aaatggttag
    30901 ataatggtta gggcaaaaaa aagcggtttt tttcaattaa tattcagtat gttgcaaaca
    30961 tggcggcatg tgtaaatccg cgatttagca tgtgtaaatc cgcgatttag catgtgtaaa
    31021 tccgcaaggg aattaggata ttttgtccta aaagcatgtg taaatccgta aattttgctc
    31081 atattttagg cgataccgaa tgttatgcac aggttatcca cagttttgat ttttaaaata
    31141 ggttttttat atttattttg tctgtttgtt ttttaatttt tatcgcatat cttgacctgc
    31201 atattttggt atttttgtca aaccggccat cggcatgtgt aaatccgcaa atggcatgtg
    31261 taagtccgca aacagtgaaa atttgctgtt ttttggcatg tgtaaatccg caaatggcct
    31321 gtttggggca tgtgtaaatc cgtgcttgtg gataatttta acgctcgacg ataatcttcc
    31381 cggataatac aaaggcagtt tttaccaact gcgcctcggt gagttcggca caaatctttt
    31441 tagcattttg attttgcgtt ttcgctgggg agttgatttg gtcgctgccg cacattgcta
    31501 aaaatgcagg aataggtata gggttgggat gtttgtggct aaagacataa tcggtgagcc
    31561 gcctagccat tggagaaagt tttttgtatt gactccaagg gatgttggaa aatgtgtttc
    31621 cggcaaaaag cacgattagt tttttatcga tgctgacttt gtaacgtgtc ggttcgcctt
    31681 tgatgttttc aattccgaca taattgtcaa taagggaaat acctccgctg acgccgtagg
    31741 tatttccgtt tttgatatag atctctgtcg ctttcattct gctcaaagat tcccggacac
    31801 gtttataata ggctccgctg gtatcccatc cgatgtcctt aataagctgc ctgatgtcaa
    31861 attcgacgta attccccaga gggaaacgac ggcagtaatc tacaagctgc atccatatca
    31921 gctcgtcatc cactgaacgg agctcggtac ctgtgtaaag gatctctact ttttctgaga
    31981 tatggaaaag tttttcacgg gagaaagttt ttctcggcac ccgatgattt cttgctgtaa
    32041 acaaacatga tcgggcgaaa tcgttcggca tcgctctatg ctttgaacca aagatagtat
    32101 ttccaaacaa atcctctata ataaggttgt cgtctgtcgg aatgcctttt gatttcaagt
    32161 attcttccct ttgggcttca tccctgcgtt cggcgactaa attcaaataa ggaagcactt
    32221 cctgaacatt tttcataatt tatcctaagt cttgttcggg gtcgtagata aatgaggggt
    32281 ctgatgattc ttcccgtccg gcagggggtt tgaggttgct gtattcaata cggctggcaa
    32341 tcatctggtc tttatagatt ttttgcccat ctttttcata gttgttattg cttatccttc
    32401 cttctatcag gataagggaa cctttgccgc aatacctgac ggcgttcttg gcctgttctc
    32461 catatacatc tatccaaaaa tagtttgtgg cctcaacttt cttcccatcc ttggtgtaaa
    32521 aatagttttc tgccaaagga atactggttg taaaaactcc tgttgcagtt tgctccaaag
    32581 tcaaatcttt ggttgttctg cctgaaatga tgaatttatt cattctgcct cctaggttac
    32641 tttgctatac ttacggttaa aaattttagc ggctaaaatt ccatgagtta ttcgaaaata
    32701 ttcttcacta atgttctgcg tcttgcatcc gaaaagaatc tgtcccacca agatttggca
    32761 gaaagggcga atttgtcccc gtcttcaatg tcgggaatta caagagggca gggcaatccg
    32821 acattggaaa ccatgtcggc cattgcttac gcccttgatg tcccgttgag ctatttgctc
    32881 gaacaccacg atcttgatcc ggagagcctt tcccttctag atggaagtca atcaacactc
    32941 ccgcctaatt acgagaagat tacggtcatt ttgccgcgtt ataaggcatt ccaagttaaa
    33001 aagtggaacg atgatgcctt aaagattttg gcaaaagaaa ccaaatagca acattcatta
    33061 ggttatttta tcccatatct gccagatatg ggcttttttt ttggctcttg aacaattaga
    33121 ctttacattt tattgaattc aatatattgt atcaactcta ttgtatggtt ctcttaatga
    33181 tggaaacgac ggaaacagca gactcccgca aagaacgggc attgaacaaa ctaaagcgtg
    33241 atttgggaaa tgagtttttg gaaaagttca tgaatccaaa cttggttgaa gtgatgttca
    33301 atccaaaaca gaccggaaac ggcgtgtctg tttactggga agagcaaggc aaggagatgg
    33361 tctatgacga gaggttgagc gattggttca aaacctcccg cgtggaagcg gttattaaaa
    33421 ccgttgccgg ttatcacggc aaagtaatca ccgaagacct tcctatcttg gagtcccaat
    33481 ttcccatcga tgggagtcgt tttacggcca tcttgccgcc ggtaaccgca tcaccctctt
    33541 ttaccatcag gaaaaaacct gcccgtgtat tcactttgga tgagtatgtc tcagcaggaa
    33601 tcatgacgga aagccagcgg cagaccatcc gcaatgcagt cagtgagcac ggaaacatct
    33661 tggttatcgg cggaacaggt tcaggtaaaa ccacgttgat taacgccatc ctctatgaca
    33721 tgtcggagca gaatcccaat gagcgtctct acatctctga ggataccggg gaaatccaat
    33781 gccattcgca aaacaaagtg caggtttaca ccacgggaag catcgatcta accaaacttg
    33841 tgaaaaccgc gctccgctac cgtcccgaca gaatcatcgt aggcgaagta aggggggcgg
    33901 aagccttgga tcttttggat atttggaaca cgggacaccc gggcggcatc gccacactgc
    33961 atgcaaacaa tgccgccttg ggactggaac gcttggaaag cctgattacc cgcaatcctg
    34021 cccatccggc agcgatacgg ccgctggtgg cgcaagtgat ccactacatc gtatccatcg
    34081 gcaaagacga gaaaaccggc cgcaaaatac gtgagatcat caaaattaag gggcttgacg
    34141 aaaagtcagg caattatgtg tttgattacc tttaagagga acagggaggc aatttaaaca
    34201 gcgggattga atactgatgt tttcataagc aacgaacgat tttttaacaa ccacgttata
    34261 tctgtgaggt aaatatatga ctctgagtca atcgcaacaa aaatggctgt tcttcatcgg
    34321 actggccgtc ttcttactgt taatgtcctc gcccgcgctc gcgtcgcaag ggcagggtgg
    34381 cggtctgccc tatgaaagct ggctgaacaa tctccgccag tccgttaccg gcccggtggc
    34441 tttcgccctg tccatcatcg gcattgtcgt tgccggtggc gtactgatct tcggcggcga
    34501 actgaacgcg tttttccgca ctttgatttt ccttgtgttg gtaatggctt tgctggtagg
    34561 agcgcaaaac atgatgtcct ctttcttcgg cggcgcggaa gttaccgctg ccgtgcaggc
    34621 cgcacagcat taccttcttc ccttggcgtc gaaaggataa aaccatgaga gccatccgca
    34681 cgatacccat ccgcagagtg gcaaaccgtc ccaacctctt ttggggtgga gatcgcgaga
    34741 tggttcaggt atcccttttg attacttttg ttttagtggt ggcggcgcaa aacacctatg
    34801 ctttcgtaat cggcttggta ttttggtttt ttgcgcttta cgcactccgg aaactggcta
    34861 aatacgatcc tttgctgcgt tttgtcggcc tgcggtcgat taccaagtac aaacgttttt
    34921 atccgcccca ctccacaccg tttagaaaca atacttcaac ccaaggaaac cgttacaaat
    34981 gatcattggg attatctcgg ccatatcggt tttgggcttg gttctgtgta cgggtctgtt
    35041 cctgaaaatc cgcgccgtta ccaatgccac caaactcaaa cagcaccgct cgaaagaaga
    35101 agggctggcc gatttgctga actatgcggc cgtaatcgat gacggcgtaa tcttagggaa
    35161 aaatggctcc ctgatggctg catggattta tcgcggcaaa gacatgggca gcagcacggt
    35221 agcggagcgc gaagacgtat ctttacgcat caatcaggcg tttgtcggct tgggcagcgg
    35281 ctggatggtg cacgtcgatg ccgcacgccg tcccgcacca aactataccg aggcaggcag
    35341 gaactacttt gacagttacg ttacagaggc catcgataac gaaagacggc aggcatttga
    35401 ggccatcggc accctgtatg aaggtttctt cgtcattgtc gctacctatt tcccgccctt
    35461 actggcagaa aaaaaggtcg ccgaactgat gtttgatgac gacaacgcca aggaaggtaa
    35521 acgcgcccag caagaccgca cactctctca tttcaaacag gcttgtgcga atctggaatc
    35581 caacctgagc atttccttgg agttgcaccg tctttgcgcc cataaagtcg ttttggaaga
    35641 cggcatggaa attacccaag acgatttcct gcgctggctg catttctgca ttaccggcaa
    35701 aaaccatccg atcaaccttc cggccgcgcc catgtacatt gacagcctga ttggtgggca
    35761 ggaactctac accggcgtcg tgccgatgat cggcgataag tttatccaag tcgtcgcgct
    35821 ggacggcttc ccgatggagt cctacccggg cattttgacg cggctcggag aaatgcctat
    35881 cgagtaccgg tggtcgaacc gcttcatctt catggatcag catcaggcgg ttgccgattt
    35941 ggagaaatac cgcaaaaaat ggaaacagaa agtacgcggt ttcttcgatc aggtgttcaa
    36001 cacccattcc ggctccatag accaagacgc tcaggcaatg gtggatgacg cggaagccgc
    36061 cttggccgaa accaaatccg gcttggtggc acagggttac tacacaagcg ttgtcatttt
    36121 gatgcacgaa gaccgggctt tcttggaaag catggcaagg caggtggaaa aagagatcaa
    36181 cactttgggc ttcaatgccc gcgttgagac ggtcaatacg atggaagcat ggttgggcag
    36241 cctgcccggc cacggaatcg aaaacgtccg ccgtccttta ctgaatacgc tgaacttcgc
    36301 cgacatgatt ccgaccagca gcatttggac gggcttggag gaaaacccct gccagttcta
    36361 tccgcccaat tcgccgccgc tgatgcaggt tgttaccagt ggctccagcc cgttccgtct
    36421 cggtctgcac gtcggcgacg tcggccacac tttggttttc gggccgactg gcgcaggtaa
    36481 atctaccctg ttggccactt tggccgctca ggccgaccgt taccggaaca tgcgggtatt
    36541 ttctttcgac aaaggccgtt ctttggaaac gctgacccgc gccgtcggag gatcacacta
    36601 caacattggc gatgaagaca acacggcttc cggtttgtct ttcgcgccca tgcagcttat
    36661 ccaaaacacg tccgaccgcg cttgggcttt gtcttgggtg gaagcggtta tggaattgaa
    36721 cggcctcaag gttacgcccc aacagcgtaa cgaaattgaa accgccatca aaaacaatgc
    36781 agccgaacag tcctattcgc tttccgatct ttcaaacacc ctgcaagacg tagagatgcg
    36841 cgaagtattg aaagcctaca ccatcgacgg ctcgatgggt tatctgctcg atgccgaaca
    36901 ggacagcttg agcttctccc gcttcacctg ctttgagatc gaagagttaa tgaacctgcc
    36961 ggaaaaatat gcgcttccga ttatgctgta tctgttccgc agaatcgaaa aagccctgac
    37021 cggcgatccc gcagtcctgt tccttgacga agcatggatc atgttggggc atcctgtctt
    37081 ccgggaaaaa atccgcgaat ggctgaaagt gctgcgtaag aaaaactgcc ttgttctgct
    37141 cgcgacccaa agcctcagtg atgcggccaa ctccggcatc ctcgatgtcc tgaacgaatc
    37201 ctgtccgacc aaaatctacc tgcccaacgc ccacgctagg gaagaggacg cttcggaact
    37261 gtacaggcgc atgggcttga atgcgcggca gattgagatt ttggccaccg ccattcccaa
    37321 gcggcagtat tactacacct cgccttacgg ccgcaggctg tttgaactgg ctcttgaccc
    37381 gctgaccttg gcctttgtcg ccgtatccga ccccgacacc ctgaatgcaa tgcggcagac
    37441 ggccgaccaa tacggggaag actggccgat ccgctggctg caaaaccggg gaatcaatat
    37501 ccataactat gtcgatgtgg acaaatacag aaggaaacac tgatgggact gaaagacatt
    37561 tttcgcgtca gggaaaaagc cgaccgcgaa atgaccgaaa acccctattt gaacgccagg
    37621 aaacaatgga acttcatcat gggcgacatg gtttcgtcgc gcctgctgtg gcaatgggcg
    37681 ggcatgaccg gcctgctgat tggtttgggc ggggtagggg gcatgattta cctcggcagc
    37741 cagtcgaaat ttatccctta tatcgtcgaa gtgaacaaat tgggcgaaac cgtggccgtg
    37801 gccagagccg accagcctca ggcaaccgat gagcggattg tccgcgccat gctcggcagc
    37861 ttcatcggca atgcccgtac cgtaaccacc gatatagccc tgcaacgcaa atacgttctc
    37921 aatacctatg ccatgctgaa tccgaaagac gcggccgcag ccaaaatgaa cgaatggctg
    37981 aacggaacgc cggagtcttc gccgttcaaa cgcgcggaaa aagaactggt cagcgtggaa
    38041 atcaagtcgg tgctgagaca aagcgacaac tcttggcagg tggactgggt ggaaaccact
    38101 cgcgcccatg acggcaaatt actcaaaccg cctcagaata tgcgtgcgct gttgaatatc
    38161 tactacgttc cgccgacaac agagcagcag attattgtga accccatcgg catgtttatc
    38221 cgtgatttct cttggtctaa cgaactgtaa caaggtacag caatgaaaag acagatttta
    38281 tccttggcgt taatcaccgc tttgtccact tccgcctttg ccgacagcta cgccgacaaa
    38341 tattttgaag gcagccacaa cccgaagctg aaccgccacg aacaaaaagg cttgggcatc
    38401 gccaaaaact actcttctga cggcaggatg gccgtaccgc ccgttcccgg cgataacggc
    38461 cgcatcacct ttgccttcgg ctctgatccg tctgtcgtct gtgccgtaat gcaggtctgc
    38521 gacattgcct tacagcccgg cgaaatgatt aacgatctgc acgccgggga taccgcacgc
    38581 tggaatatca ctccggcaat ttccggcagc aacggcaacg aaagaatgca catcttggtt
    38641 aaaccactgg atgtcggttt gcaaacgacg cttttcatag ccaccgaccg cagaacctat
    38701 catatccgcc tcaaatcaca ccgtacccgt tatatgcctg ccgtcggttt ttcgtatccc
    38761 gaagacaccg ccgcgcgttg gcgggcgatc cgggcaaacc aagcccggca agccgaagct
    38821 caggttctgc ccaccggaga aaacatccaa aacttggatt tcaactacac catcagcggc
    38881 cacaatccgt catggaaacc tttgcgcgtt tataacgacg gcacccgcac catcatccaa
    38941 atgccgccga ctttatccca aggggaagct ccggccttgc tggtcgtccg taacggcgaa
    39001 aacaccttgg tcaattaccg gcttcacgaa gaccgctaca tcgttgacag cctgtttgac
    39061 gaagccatca tgatcaccgg cgttggccgc catcaaacca aagtcgttat cagacggagc
    39121 cgatcatgaa atcaaatgtc ctgttcgtcg cgctggcttt ggccgttggc ggatgcgcca
    39181 ccgggcaata cggttctttc gtccgttcag acgccgtatc cgcctcagta actcaagatg
    39241 tagtggaaaa actccaagaa gaatttccgg cggccaacac cgttttcttc ctgaaccgca
    39301 gcaaatccga taccttcggt acgcaactgg atcaggcatt gcgtgaaaaa ggctttgcca
    39361 caaccgccca acagggcgaa aacgaaaaat cactgaatta cgtcttggat caggttgatt
    39421 ccgatattta ccgggtaatg atctacattg acggcacgcc ctattcccgt gcctaccgcc
    39481 aaaccgaaaa cggcgccgtg gttccgaccg gttcttggac taaacgtgga gactgaagca
    39541 tgagccaaag caccgaagat ctgctgaacg catcccgttc cccttcgatc gcaccggata
    39601 cttccggccg tcgtcccggt gtgcgccgcc tgaacaacat tccgatctat atcgtcggcg
    39661 gtgttgttct cggattcgcc ctgctgattg cactggtggc agcaactaaa ggccaacagt
    39721 ctgccgggca ggcaacgcag ggagacggta aggatgcaac aagaatggcc gattccctat
    39781 tgaacgaact gcctcaggaa ggcggtctga ttaccgccaa agcatccgaa ccggccatgc
    39841 ccgccctgcc tgacggcatg gcgtccgcgc ctgatttacc ggcatccgcg cccacatctt
    39901 taccgatggt tcccgatatg gccaaccttc cggccacacc tcccgaagtg gatcaggaag
    39961 cggaacaaat ccggcaggca aaagcgcaga tgttccaagc agccctagct gccccaacca
    40021 aagtggatgt cgtcgcatcg acttctcggg gttcgtccgg cctatatact gctgccttat
    40081 cttcttccgg ctcaggtaca cccaaccgta gcgagatgct ggctcaaatc gccgaagtgc
    40141 agcgcatgtc cgagaatgcc ggttccaatc ccgccgaagt ggagcgcgtt tacaaccaac
    40201 aactggccaa tgcccaacag atggccgccg caggtggtca ggacggcgta caaggggcag
    40261 gcggcatgcc cggcctgaac acacctccga ctcccggcaa tatcccgaga caaacggcac
    40321 aaaacggtgg aaacaactgg cggcttgaga gccaagtcga acgtccgaac gcctacatga
    40381 tccgcagcgg tgcggttatc cctgcaaccc tgatcagcgg catcaattcc gatctgcccg
    40441 gcatggttca ggcacagatc tcacaaaacg tgtatgacac cgccaccggc cgtcatctgc
    40501 ttttacccca aggatcgcgc ttggtcggca cttacagcag cggcatcaat cttggccagc
    40561 gtcgcctgtt tatcgcgtgg aaccgcatta ttttccctga cggtaaagcc ttggacatcg
    40621 gcgatatgcc cggagccagc ggcgcaggct acgccggatt cagcgaccgc atcaacaacc
    40681 attacggccg tctgtggggc aatgccctga tgctctccct agtcggagcc agcatcactt
    40741 acggcgtgga caaaaacagt aacaacaata acaacggcaa taacaacggc aacaactcca
    40801 ccgtgcgcgg agccttgtcg gagtccttgg gtcagacctt cggacaggcc gtgtcccaaa
    40861 gtattcagaa aaacatgaat gtttccccga cgttagagat ccgcccgggg taccgcttca
    40921 atgtcattct tactaaagac attgatttct ctaagcccta tcgttccttt gactattaac
    40981 aggagagcat tatgcaaaaa cgtcttttaa gcaaaaccaa gaccgtagcc ctgtctttgg
    41041 ccgtttccgt gtccttgggg ctgtccgcac cagcatacgc aggcatcccg gtaacggatg
    41101 gcggcaacat tgcccaaagt tcggtctccg ccattcaaaa tgtggcgcaa accctgaagc
    41161 agattgagga gtaccgaatc caactgaaac agtacgaaac ccagttgcgg aactccttgg
    41221 ctccggcggc ttatgtttgg gatcaggcca acaaaactat ggccaacatg aaggccgctg
    41281 tcgcccgctt gcgagcctac aaacagcaac tgggtaacat ggaagagtac ctgagcaaat
    41341 tccaagccgt ggacttctac aaaagctctc cgtgctttac cgccaaaggt tgctctgcgg
    41401 ccgaccgcgc caaactcgag caaaactacg cccaattgtc gcgcatgcag aaattatcta
    41461 atgacggcct attaaggagc atcaataacg agatcgactc catcagcaga gacgccaaca
    41521 ccttaaccca actccaacga aacgcccaat cagccaccgg ccagttggag gcactacaat
    41581 acgcgaacca gttccatagc gctcaagcgc atcaactgct tcagttgcgt aatatgatta
    41641 atactgagat gcaggcaaat gctgcttatc gtcaaagtca acttgataga gaggctagat
    41701 atgaagctgc tcgtaaacaa gttaggacgt cggatatttt gaaacaccct tatagaagag
    41761 ggcaataaaa atagggtttc taatgtgtaa taaacattag aaacccactc taaatttgaa
    41821 agattacctc atgagaactt tattttttgt attgctaagt agtttcatgc ttgttgcgtg
    41881 ccaacaaaag gatgagaacc aacaacgcag agaagaacta cgtaaagtag atcaggatgt
    41941 cctaaaacgg ccatacaaaa ggaaagagta gttattttct tatgcaatat ataacccaaa
    42001 ttatg
//
LOCUS       DAXFZR010000005        41628 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000005 DAXFZR010000000
VERSION     DAXFZR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41628
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.005"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..293)
                     /locus_tag="ACFJAS_000192"
     CDS             complement(<1..293)
                     /locus_tag="ACFJAS_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3981554.1"
                     /translation="MIYKNKIKEDGQDERLKEKAAQYRLFENNDTFQTALKPKFTFID
                     LFAGIGGFRIAMQNLGGEYVFSSEWDEKAKLTYEANFGEVPFGDITLEEIKQY"
     gene            complement(295..708)
                     /locus_tag="ACFJAS_000193"
     CDS             complement(295..708)
                     /locus_tag="ACFJAS_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC3981555.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(725..1714)
                     /gene="pheS"
                     /locus_tag="ACFJAS_000194"
     CDS             complement(725..1714)
                     /gene="pheS"
                     /locus_tag="ACFJAS_000194"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC3981556.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(1955..2314)
                     /gene="rplT"
                     /locus_tag="ACFJAS_000195"
     CDS             complement(1955..2314)
                     /gene="rplT"
                     /locus_tag="ACFJAS_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC3981557.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(2327..2524)
                     /gene="rpmI"
                     /locus_tag="ACFJAS_000196"
     CDS             complement(2327..2524)
                     /gene="rpmI"
                     /locus_tag="ACFJAS_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC3981558.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(2671..3192)
                     /gene="infC"
                     /locus_tag="ACFJAS_000197"
     CDS             complement(2671..3192)
                     /gene="infC"
                     /locus_tag="ACFJAS_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC3981559.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(3210..5123)
                     /gene="thrS"
                     /locus_tag="ACFJAS_000198"
     CDS             complement(3210..5123)
                     /gene="thrS"
                     /locus_tag="ACFJAS_000198"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC3981560.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWRTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(5176..5252)
                     /locus_tag="ACFJAS_000199"
     tRNA            complement(5176..5252)
                     /locus_tag="ACFJAS_000199"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5216..5218),aa:Val,seq:gac)
     gene            5431..6546
                     /gene="tgt"
                     /locus_tag="ACFJAS_000200"
     CDS             5431..6546
                     /gene="tgt"
                     /locus_tag="ACFJAS_000200"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC3981561.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPMLPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKPDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAVEQGKFADWQAQFHENRARGVD"
     gene            6556..7638
                     /gene="hemH"
                     /locus_tag="ACFJAS_000201"
     CDS             6556..7638
                     /gene="hemH"
                     /locus_tag="ACFJAS_000201"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC3981562.1"
                     /translation="MPSEAASDSILPPYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALTGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            7829..8296
                     /locus_tag="ACFJAS_000202"
     CDS             7829..8296
                     /locus_tag="ACFJAS_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
                     /protein_id="HFC3981563.1"
                     /translation="MNTTRLPTALVLHFLCAAASAADNSIMTKGQKVYESNCIACHGK
                     KGEGRGTAFPPLFRSDYIMNKPHVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKNQTDKMPSETGNPASDGIQTKPF"
     gene            complement(8293..10014)
                     /locus_tag="ACFJAS_000203"
     CDS             complement(8293..10014)
                     /locus_tag="ACFJAS_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFC3981564.1"
                     /translation="MNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAGEEG
                     VGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATVLPL
                     GLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGANDP
                     MAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNLAEG
                     LYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAWLAQ
                     ATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYISW
                     LGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVAMPN
                     KPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIRNGS
                     RIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAARSG
                     DLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGDGNIGS
                     MGLKVPR"
     gene            10218..10958
                     /locus_tag="ACFJAS_000204"
                     /pseudo
     CDS             10218..10958
                     /locus_tag="ACFJAS_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            11027..12565
                     /gene="lnt"
                     /locus_tag="ACFJAS_000205"
     CDS             11027..12565
                     /gene="lnt"
                     /locus_tag="ACFJAS_000205"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC3981565.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            12829..13692
                     /gene="rpoH"
                     /locus_tag="ACFJAS_000206"
     CDS             12829..13692
                     /gene="rpoH"
                     /locus_tag="ACFJAS_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC3981566.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(13849..14235)
                     /locus_tag="ACFJAS_000207"
     CDS             complement(13849..14235)
                     /locus_tag="ACFJAS_000207"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFC3981567.1"
                     /translation="MIFASRWLQVPIYAGLTVVRAICAYKFLKSLKHLVMNLDVSDEN
                     AIMLAVLNLIDVVMIANLLTMVQIGGYESFVSRLRIDDHPDRPEWLSHVNAPVLKVRL
                     SMSIIGIHPSICSKHLSIPPICRKSS"
     gene            14425..15309
                     /locus_tag="ACFJAS_000208"
     CDS             14425..15309
                     /locus_tag="ACFJAS_000208"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC3981568.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENSIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(15400..15915)
                     /locus_tag="ACFJAS_000209"
     CDS             complement(15400..15915)
                     /locus_tag="ACFJAS_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC3981569.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(15991..16410)
                     /locus_tag="ACFJAS_000210"
     CDS             complement(15991..16410)
                     /locus_tag="ACFJAS_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981570.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(16531..17529)
                     /gene="holA"
                     /locus_tag="ACFJAS_000211"
     CDS             complement(16531..17529)
                     /gene="holA"
                     /locus_tag="ACFJAS_000211"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC3981571.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(17531..18010)
                     /gene="lptE"
                     /locus_tag="ACFJAS_000212"
     CDS             complement(17531..18010)
                     /gene="lptE"
                     /locus_tag="ACFJAS_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC3981572.1"
                     /translation="MNKIFLTAAALVLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(18062..18841)
                     /gene="pgeF"
                     /locus_tag="ACFJAS_000213"
     CDS             complement(18062..18841)
                     /gene="pgeF"
                     /locus_tag="ACFJAS_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC3981573.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILRREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            19295..20269
                     /locus_tag="ACFJAS_000214"
                     /pseudo
     CDS             19295..20269
                     /locus_tag="ACFJAS_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(20396..21520)
                     /gene="rluD"
                     /locus_tag="ACFJAS_000215"
     CDS             complement(20396..21520)
                     /gene="rluD"
                     /locus_tag="ACFJAS_000215"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC3981574.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPPELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MDLDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            21519..22322
                     /locus_tag="ACFJAS_000216"
     CDS             21519..22322
                     /locus_tag="ACFJAS_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC3981575.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(22392..24611)
                     /locus_tag="ACFJAS_000217"
     CDS             complement(22392..24611)
                     /locus_tag="ACFJAS_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC3981576.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGV
                     GTVGADRVLLHDGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRRQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            24643..24918
                     /locus_tag="ACFJAS_000218"
     CDS             24643..24918
                     /locus_tag="ACFJAS_000218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981577.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            24997..29778
                     /locus_tag="ACFJAS_000219"
     CDS             24997..29778
                     /locus_tag="ACFJAS_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFC3981578.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWGASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLDQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADSNQKVQAFSQVGIVSGRGTLVLNSPDQINPNNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTDHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     NYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENEKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLNQNAALVLGKAALWGKIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKKSASGHY
                     QLHVQNKTGEPNQERLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEALR
                     QQAKAEQVKRQQAEAGRKSAELSAKQRAGEEERRQTAQSQPQRRKRRAAPQDYMAVSQ
                     DRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQRAEAEREAQALAARRK
                     AEAEEAKHQAAELAHRQEAKRKAAESAKRKAEEEEHRQTAQSQPQRRKRRAAPQDYMA
                     VSQDRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEGIPLDALEDEDVS
                     ESVDTSDKQPQDNTELHEKVEAVSLQPRAAQPRTQAAAQADAVSTNTNSALSDTMAST
                     QSILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGI
                     DRSLSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGDIGAGSL
                     RSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGDDSVK
                     VSSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAYKADNQQ
                     QYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(29854..31056)
                     /gene="trpB"
                     /locus_tag="ACFJAS_000220"
     CDS             complement(29854..31056)
                     /gene="trpB"
                     /locus_tag="ACFJAS_000220"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC3981579.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEATGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(31139..31768)
                     /locus_tag="ACFJAS_000221"
     CDS             complement(31139..31768)
                     /locus_tag="ACFJAS_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC3981580.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            32044..32823
                     /gene="rsmA"
                     /locus_tag="ACFJAS_000222"
     CDS             32044..32823
                     /gene="rsmA"
                     /locus_tag="ACFJAS_000222"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC3981581.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGISGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            32838..33407
                     /locus_tag="ACFJAS_000223"
     CDS             32838..33407
                     /locus_tag="ACFJAS_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC3981582.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            33518..33973
                     /locus_tag="ACFJAS_000224"
     CDS             33518..33973
                     /locus_tag="ACFJAS_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981583.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            34015..34743
                     /locus_tag="ACFJAS_000225"
     CDS             34015..34743
                     /locus_tag="ACFJAS_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3981584.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            34816..35175
                     /locus_tag="ACFJAS_000226"
     CDS             34816..35175
                     /locus_tag="ACFJAS_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981585.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            35180..35620
                     /locus_tag="ACFJAS_000227"
     CDS             35180..35620
                     /locus_tag="ACFJAS_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC3981586.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            35651..36925
                     /gene="folC"
                     /locus_tag="ACFJAS_000228"
     CDS             35651..36925
                     /gene="folC"
                     /locus_tag="ACFJAS_000228"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC3981587.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            36939..37979
                     /gene="ftsN"
                     /locus_tag="ACFJAS_000229"
     CDS             36939..37979
                     /gene="ftsN"
                     /locus_tag="ACFJAS_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC3981588.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRTAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            37972..38469
                     /locus_tag="ACFJAS_000230"
     CDS             37972..38469
                     /locus_tag="ACFJAS_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC3981589.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            38663..40213
                     /gene="purF"
                     /locus_tag="ACFJAS_000231"
     CDS             38663..40213
                     /gene="purF"
                     /locus_tag="ACFJAS_000231"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC3981590.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYRTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     ISDAGDEE"
     gene            40313..40804
                     /gene="greB"
                     /locus_tag="ACFJAS_000232"
     CDS             40313..40804
                     /gene="greB"
                     /locus_tag="ACFJAS_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC3981591.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            40863..41489
                     /locus_tag="ACFJAS_000233"
     CDS             40863..41489
                     /locus_tag="ACFJAS_000233"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC3981592.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFINESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT        10702 a        11828 c        10116 g         8982 t
ORIGIN      
        1 atgtattgtt tgatttcttc caaagtaata tcgccaaacg gcacttctcc gaaattggct
       61 tcgtacgtca gtttggcttt ttcgtcccac tcgctcgaaa acacgtattc cccgcccaag
      121 ttctgcatcg cgatgcggaa gcccccgata cctgcgaaca agtcgataaa ggtaaatttg
      181 ggtttcaagg ccgtctgaaa agtatcgttg ttttcaaaaa ggcggtattg tgctgctttt
      241 tcttttaacc gttcatcctg cccatcttcc ttaatcttgt ttttgtaaat caattcatct
      301 tgtttggatg atttctttga ctttttcggc acagcaggtt gtatttttga cgacatcgtt
      361 actccaaaaa cgtaaaaccg tccagccctc ggctttcaga cggcctgtta cttctatatc
      421 cctttggata ttgcgctcaa ttttggcaat ccaaaactca cgatttccct ttattaccgc
      481 ttttttctgt tcccaatcct taccgtgcca gaattcccca tcgacaaata cggcaacttt
      541 atatttttta aatgaaaaat caggttttcc aaaaatgctt ccgctatttt tccgatacct
      601 gagccccaaa gcccacattg ccttcgccaa cacaagctcg ggtttcgtcc ctgtactttt
      661 gttggactgc atacattttt tgcgctgctc cggggttaat ttatccattg ggtttatttg
      721 agatttattc aaactgcttc aagaagttca aatcgttatc gaagaacaag cgcaggtcat
      781 tgacgttgta gcgcaacatg gcaaagcggt cgagaccaat accgaaagcg aaaccggtat
      841 atttttcagg gtcgatattg acatttttca acacattagg atgtaccata ccgcaaccgc
      901 ctacttccaa ccatttgccg ttttctccca tgatgtcgat ttcggcagaa ggctcggtaa
      961 atgggaagaa agacggacgg aaacgcactt gtaggtcatc gcgttcaaag aagcgacgga
     1021 taaagtcagt gaacactgct tttaagtcgg caaaagttac gccctcttct acccacaaac
     1081 cctcggcctg atggaacata ggcgagtgcg tggcatcgct gtccacacgg taaacgcggc
     1141 cgggggcgat aatgcggatg ggcggctctt ttttatcgag catatagcgg atttgaatcg
     1201 gggaagtgtg cgtacgcaaa acatcgccgt tttcaacgta aaacgtatcc tgcatcgcac
     1261 gggcaggatg gtttgcaggg atgttcaggg cttggaaatt gtgaaaatcg tcttcgattt
     1321 caggcccgtc cgccacttcg aaacccattc cgtgaaagag ttcgaccaca cgttgcaagg
     1381 tcagggttac gggatgcagg ctgccgcctt cctgagcgcg tccgggcagg gtaatgtcga
     1441 gggcttcggc ggcaagtcgg gcttgcagct tggcttcgtt gagggcatcg cgtttggcat
     1501 taaaagccgt ctgaaaccgg tttttgcatt cattgatatg cgcacctatg gttttgcgct
     1561 cttcaggcga catttgcccc aaagttttca gaagtccggt caactcgccg gttttgccaa
     1621 gataacgggc tttgatttgt tctagagcgt tgaagtcttg cgcagcttct actgcggcaa
     1681 tgccttctgc aacgatacgg tttacatttt ccataatatc aagcctgtca attaatgata
     1741 tgccacctga aacaacgagg ccgcctgtat agcctgacat atcagactct tcagacgacc
     1801 ttattttctt cacggccaat ccattggata aattttctgt attttaacgc aaatttattt
     1861 aaaaaaatag actttaaaga aagaaaatca atcacataga atttccgcaa acaaagaaaa
     1921 aaggaagccg cagcttcctt ttcaattttt tggattaagc agccaaagca gctttggctt
     1981 tttcaaccaa ttgtgcaaaa gcggctttat cgaacacggc caaatcagcc aatactttgc
     2041 ggtcgatttc gatagaggcg cgtttcagac cgttcataaa tttgctgtaa gacaacccgt
     2101 tttcacgcgc acctgcattg atacgaacaa tccacaattg acggaattgg cgtttgcgtt
     2161 ggcggcggtc acggtatgcg tattgaccag ctttcattac cgcctgcttg gcaacgcggt
     2221 aaacgttttt acgacgacca cgatagcctt tggctaacgc gaagattttt tggtgacggg
     2281 cacgggcggt aacaccgcgt tttacgcgtg gcatattcta aactccttaa gcgtagggta
     2341 acattttagc aacagaagcc aaatcgcgat catttaccat agaggtaccg cgcagttggc
     2401 gtttgttttt ggtggtcttt ttagtcaaga tgtggcgttt gaacgcatga gcgcgtttca
     2461 caccgccgtt acccagtact ttaaagcgtt ttttcgcgct agacttggtt ttcattttag
     2521 gcatgggaaa actccattcg ttatcggata aggcattagg gggttttaaa acatcggttt
     2581 caaacacttg aacccacaat gccacggttt tttgccgcaa ttgaaaactt actccgaagc
     2641 ggcaatcgga gtaagcggaa aattatagct ttattttttc ttcggcgcaa tcatcatcac
     2701 catttgacgg ccttccattt tgggaaagga ctcgatttgc gccacttcag ccaaatcttc
     2761 ttttacacgt tccaaaagtt gcgcgccgag ttgctggtga gccatttcac ggccgcggaa
     2821 acgcaatgtc actttgactt tatcgccgtc ggcaaggaag cggttgatgt tgcgcatctt
     2881 gatttgataa tcgccctcat ctgtacccgg acggaatttg atttccttaa tttgcacctg
     2941 cttttggttt ttcttggctt cgtcgcgctt cttggcttgt tggtatttgt atttaccgta
     3001 atccatcagt ttgcacacag gaggtttagc agttggggaa atctctacca aatcgacatc
     3061 ctgcccttcg gccatagcca aagcttcacg aactgaaacg acaccaagct gttcgcctga
     3121 ctcactgatt aaacgcactt ctttggcggt aatttcaccg ttgattcgtg cttcgcgttc
     3181 ttgagcgatg atgaatactc ctataaaaat taatgattga cgagggcatc agtgatttct
     3241 tgctgcaatt gcgcaatgaa atcatccaaa tccaaagaac ccaagtcttc cgctttgcgg
     3301 cgtaccgcaa ctttgttttc ttgtttctct ttatcgccga taacgatttg ataagggaaa
     3361 cggtattggc tgttgtcgcg gattttgtaa ccgatttttt cattacgcaa atccaactcg
     3421 gcgcggaagc ctgccgcctg caatttggca gcaacttccc gacaataatc tgcctgattt
     3481 tcggtgatgt tcataatcac catttgaacc ggtgcaagcc ataacgggaa agagcctgca
     3541 tggttctcaa tcagaatgcc gacaaaccgc tccaaagaac ctaaaatggc gcgatgcaac
     3601 ataacaggcc gcgctcggtc gttgttttct gttacatatt ctgcatccaa gcgttccggc
     3661 aagacaaaat ccagttgtaa tgtaccgcat tgccaagaac gacccaaggc atctttgacg
     3721 tgatattcga ttttaggccc gtaaaacgca ccctcgcccg gcaattcgcc ccattccacg
     3781 ccgcaggcag tcaatgcctc gcgcaaaccc tgctctgcct tatcccacac gtcgtctgaa
     3841 cctgcgcgtt tttcagggcg aagggaaagc ctgacggata catcatggaa accgaactgt
     3901 ttgtagatgc gaaccaacaa ttcattgaac gcacgagcct cgctgacgat ttgatcttcg
     3961 gtacaaaaaa tatgcgcatc atcctgcaca aaaccgcgaa ccctcatcag accgtgcagt
     4021 gcgccgctcg gctcgttgcg gtggcaagaa ccgaattccg ccaaacgcat cggcaaatct
     4081 cgatatgaac gcaaaccgtt gttgaaaatt tgaacatgac ccggacagtt catcggttta
     4141 accgcatatt cgcgtttttc cgaactggtt acgaacatat tgtctttgta gttgtcccag
     4201 tggccggatt tttcccaaaa ggttttatcc atgatttggg gggttttaac ttccttgtaa
     4261 ccggcggcat tcagctcttt acgcatatgc tgctcgatag tccgccacaa agcccaacct
     4321 ttgggatgcc aaaacaccat acccggcgct tcgtcctgca agtggaacaa atccagttgc
     4381 ttgcccaatt tgcggtggtc acgtttttct gcttcttcaa tgcgttggat atagtctttt
     4441 aattcgtctt tcgtcgccca agcagtgcca taaatacgtt gcagcatttc attattgctg
     4501 tcgccgcgcc aatatgcgcc tgccagcttg gtcagcttga agtttttcag gaagcgggtg
     4561 ttcggaacgt gcggaccgcg gcacatatcg acatattcct gatgatgata catacccatt
     4621 gcttcaactt cgggcatatc ttcaatcagg cgcagtttgt attcttcgcc gcgctcttga
     4681 aaaattttaa tcgcctccgc acgcggagtc atgattttga ccacatcata atcttgggca
     4741 atcaattctt tcatacgcgc ttcaatggcg gcaacatctt ccggtgtaaa cggtttttcc
     4801 gtggcgatgt cgtaataaaa gccctcttca atgacggggc cgataaccat ttttgcatta
     4861 ggatagagtt gcttgacggc atgcccgaca agatgtgcgc aggaatggcg gatgatttcg
     4921 atgccttcct gatctttcgg agtaatgatt tgaacagcag aatcctcaac aatcgggtcg
     4981 cacgcatcga ccaatttgcc gtttaccttg cctgccaccg ccgccttcgc caaaccggca
     5041 ccgatagacg cagcaatttg agccacggta acgggggatt cgtattggcg gactgaacag
     5101 tccggcaagg taatattcaa catcaaacgc tccgaaagat taaaaaataa cggcataaat
     5161 gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc accatgtcaa
     5221 ggtggtgctc taaccaactg agccacgtgc cttcaaagaa tttttgtatt ttatcgggtc
     5281 gttttaattt ttgcaagaga gatgtgcatt tctttatgca acattgtggc aagatttggg
     5341 gataccgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa atatatttcg
     5401 acccctgtcg cccaacttac attcaatctt atgcttaaat ttactttaca caaaaaagac
     5461 ggtctcgccc gacgcggcac gctggagttg aaccacggaa aaatcgaaac gccggttttc
     5521 atgccggtcg gtacttacgg ttcggtcaaa gcgatgaacc cgcaaaacct gcacgacatc
     5581 aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg tttggaggtc
     5641 gtcgaacagt ttggagggct tcacggtttt atcggctggg acaaaccgat tctgaccgac
     5701 tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgaccga agaaggctgt
     5761 acgtttaaaa gcccgattaa cggcgacaag ctgtttttat cacctgaaat ttccatgaaa
     5821 atccaaacgg ttttgaactc ggatatcgcg atgcagttgg acgaatgcac gccgggcgaa
     5881 acaacgcgcg aacaggcgcg aaaatccctg caaatgagct tgcgctgggc ggaacggagc
     5941 aaaaaagcct ttgaagattt gaaaaacccg aacgcgctgt tcggcatcgt gcaaggcgcg
     6001 atgtatgagg atttgcgcga agaatctttg cgcggtttgg aggaatttga ttttcccggc
     6061 cttgccgtcg gcggtttgtc cgtcggcgaa cccaagcccg aaatgtaccg tatgctgcac
     6121 gccgtcggcc cgatgctgcc cgaacacaaa ccgcattacc tgatgggcgt aggcacgccg
     6181 gaagacctcg tgtacggcgt ggcgcacggc atcgatatgt tcgactgcgt gatgcccacc
     6241 cgcaatgcgc gcaacggctg gctgtttacc cgtttcggcg atttgaaaat caaaaacgcc
     6301 aaacacaagc ccgacaagcg tccgatagac gaaagctgca cctgctacgc ctgccaaaac
     6361 ttcagccgcg cctacctgca ccatctgcac cgcgccggcg aaatcttggg cgcgcaattg
     6421 aacaccatcc acaacctgca tttctaccaa gtcattatgg cggaaatgcg cgatgccgtc
     6481 gaacaaggca aatttgccga ctggcaggcg caattccacg aaaaccgcgc gcgcggcgtg
     6541 gactgattcc cggcaatgcc gtctgaagcc gcttcagaca gcattttgcc gccttaccct
     6601 acctgcacta tttttcagaa accgatcatg cttccattcc ttccagaacc ctccctttcc
     6661 tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacacc cgacgcaccg
     6721 accgcacagg cggtacgccc ttatctgaaa tcctttctga ccgaccggcg catcgtcgaa
     6781 ctgcccaaat ggctgtggta tcccattctg catggcctgg tactgacgtt ccgccccaaa
     6841 aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgcccct agaagtttac
     6901 acagcacgcc aagctgccgc gcttgccgaa cgtatgcccg acctcatcgt ccgccacgcc
     6961 atgacttacg gcaatccttc gatagcggat gtattggcgg agctgaaatc ccaaggtgta
     7021 ggcagattgc ttgcgattcc cctatatccg caatatgccg cttccagcag cggagcggcg
     7081 gtagataaag tgtgcgaaca actgctgctg caacgcaacc aaatgagcgt ccgcaccatt
     7141 tcgcgctttt atgatgatgc cggctacatt gatgcgatga aaaaccacat cctccgatat
     7201 tgggcggaac acgggcgtgg gaaaaaactg atgttgagtt ttcacggcgt accgcagaag
     7261 cactacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa actgcttgcc
     7321 gaagcactgg aactgaccga agacgaatat accgtatcgt tccaaagcca attcggcagg
     7381 gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa acagggggta
     7441 accgagctgg acgtattctg cccgggcttt ttggcagact gcttggaaac catggaagaa
     7501 atcgccctga cggggcggga acagttttat gaagcgggcg gaaaaaacta ccgctacatc
     7561 ccctgcctca acgacaaccc cgactggata gacgcactcg tcgcacttgc cgaagaaaac
     7621 cttggcggct ggcgttgaaa ctgtttgagc catgtctaaa atgccgtctg aacgccgtgt
     7681 tcagacggca ttttcccgct actttagcgt atgatggcat ctatgccgga cagcacatca
     7741 agtgcaacct tactaccgat tcggcaaaaa aacctttcac ccgctacgca aaatcaacaa
     7801 caaggtttca aacattaagg aaacgccaat gaacacaacc cgactgccga ccgccctcgt
     7861 cttgcacttc ctctgcgccg ccgcttctgc cgccgacaac agcatcatga caaaagggca
     7921 aaaagtgtac gaatccaact gcatcgcctg ccacggcaag aaaggggaag ggcgcggcac
     7981 tgcgtttcct ccgcttttcc ggtcggacta tattatgaac aaaccgcacg tcctgctgca
     8041 cagcatggtc aaaggcatca acggtacaat caaagtcaac ggcaaaacct acaacggatt
     8101 catgcccgca accgccatca gcgatgcgga cattgccgcc gtcgccactt atatcatgaa
     8161 cgcctttgac aacggcggcg gaagcgttac cgaaaaagac gtaaaacagg caaaaggcaa
     8221 aaaaaaccaa acagacaaaa tgccgtccga aaccggcaat ccggcttcag acggcattca
     8281 aaccaaacct ttctaacgcg gtactttcag acccatagac ccgatattgc cgtccccgtc
     8341 aacctccttc gcggtcagca taaagccgcc gatgtcaata cggtcgccct cgaccggctc
     8401 ctgactgtcg gaacgcttat cgaaaagctc ggcaaggctc aaaccctctt cgcccgcttc
     8461 cagcttcaaa ccgtaagcaa gtgccaaatc gcccgaacgt gctgccggcg aaacgacaaa
     8521 ctcaccgaag aaatcaaaat tttcacggac gccgataccc gtctcggtaa agtatttcgc
     8581 cattttatcg accttgccgt cgggcaggat gtaccacgcc aaatcctccg cctgaagcac
     8641 ggtgtcggac tgcatttcga ttcggctgcc gttgcgtatc aatgcgaaac agcgtgcgtc
     8701 aaacgaatcg gaaatcggtt cgaccgtatc gggatggtgt ccctccgctt cagattcggc
     8761 aaccacttta aacgccgaca ttctgacaat ttccttttcc gccagccaaa tatcatgcgt
     8821 atctttcggt tcgggcttgt tgggcatggc aaccttcaac agccgcgcca tcacaggaat
     8881 cgtcgttccc tgaatcagca gcgacagcac caccacggca aacgccacat caaacagcag
     8941 gtgcgaattg ggaacgccca tcaccagcgg catcatcgcc agcgaaatcg gtacggctcc
     9001 tcgcaagccc aaccaactga tatacgcctt ttcacgcagg ctgtaattga atttccacaa
     9061 accgccgaac actgccagcg gacgcgcgac cagcatcagg aacgccgcaa tcgccaaggc
     9121 ttccgccgcc ctgtccaaca cgccggcggg agaaaccagc agaccgagca tgacgaacaa
     9181 agttgcctgc gccagccaag ccaaaccgtc catcacacgc aaaacgtgtt ccgtcgcacg
     9241 gttgcgctgg ttaccgacaa tgatgccggc aaggtaaacc gccaaaaagc cgctgccgcc
     9301 tatggtattg gtaaacgcaa acacaagcag cccgcccgac acaatcatca gcgcgtacag
     9361 accttccgca agattcagac ggcgtaccag ctttgccaat atctttccgc ccgcccaacc
     9421 cgtcagcaga ccgaaaccga tttgcagcgc aagcatccgg acaaacgctg ccgcacccga
     9481 ttccgccggc tgcataatca tggtaatcag tgccgtaacc aaaaaaaccg ccatcgggtc
     9541 gttcgcaccc gattcgattt ccaaagtcgc ctgcacccgt tcgttcaaac gcacgccgct
     9601 gttgcgcaaa aggctgaata ccgcgccggc atcggtcgaa ccgacaatcg ccgccatcag
     9661 cacgccgaac ttccaatcca aaccgagata aaaagttgca aacagtccca gcggaagcac
     9721 agtggcaaac acgccccacg aagcgagtac cgacgcgggc ttcaacgcaa tccgaaaact
     9781 ggaaagctgc gtccgcaaac cgccgtcgag cagaataacc gccaaagcaa gctggctgat
     9841 gaaattcgcc atcacgacat tattgaaggc aatgccgcca acgccttcct cgcccgccag
     9901 catgccgacg gcaagaaaca ccagcagcaa aggcattccc aaacgtgcgg acaaagtggt
     9961 cgaaaccacg ctcaggaaca acagtgcgcc acccagcaga aacaggctgt tcatccaatc
    10021 cacgccaacg gctccccctt aaaattcatg aaattagaat cgaaagattg taacacggca
    10081 ttttcttctc acgaaacttt atttcatgtc cgccaccgcc ttcagacggc attttcctgt
    10141 ttcatgccgc ctgataccgc ccaaacccgc gttctcctat ataatgtcgg actttgcgcc
    10201 gcatttcagg attttttatg gcatttgcct cactatttac tctgccggac tacatcaccg
    10261 ccgttttgga cgatgttgcc ctgatgacca aagccgccgc aaagaaaacc gccggcgtgg
    10321 ccggcgacga tttgacaccc aatgccaacc gggttacagg cgtatcggca gaacgcgagt
    10381 tgccgattgt ttggccggtg gcgaaaggtt cttttgccaa caagccggtt ttggtgtctg
    10441 cggctctgct gctttccgcc ttcctgccgc aactcattac gccgcttttg acggcaggcg
    10501 ggatttattt gtgctttgag ggcgtggaaa agctgctgca caagtttctg caccgacatg
    10561 acgcgcacga tgacggcggc gaaacggcgg ttaacgagca ggctaaaatc aaaggtgcaa
    10621 tccgtaccga ctttatcctg tccgcagaaa tcatcatcat cgcgctgtgc gtggtcgaac
    10681 aatacagcct gatgacgcgc tcgctcgtga tggcggcaat cggcatcggc atgactgttt
    10741 tggtgtacgg aatcgtcgcc gtcatcgtca aactcgacga cttgggcatg ctgctgatgc
    10801 gccgcccaca acctcggcct tttgcacgat ttcctgcacg cgcaacactg gaacgctggc
    10861 tggacagagt attccgccaa cctcgttgtc gggccgcttt ccggttcgat tgcctgcgcc
    10921 ccgccttgtc gctgatgaat cgtttcgtca ggcattgatt cctttttcaa ataccgatgc
    10981 cgtctgaaag acgttcagac ggcatattcc gaacagacag acgaatatgt tttccaaact
    11041 ggacaaatac tggcagcacc ccgccctcta ctggcctttg ctcatccttt ttgccgccgc
    11101 cacccccttc gccttcgcac cctactacca cttttggctg atgcccttga ttttcggcgc
    11161 cttcgtccgc ctcatcgaac tgcgtccgcg ttttgccgcc tcttccgcct acctgttcgg
    11221 tctgaccgca tacacggcac agttctactg gatacacact gccctgcacg acgtttccgg
    11281 cctgcccgac ctctatgccg taccgctgac cttcctgctg cccgcctacc ttgcccttta
    11341 tccggcactg tgtttctggc tgtggaaaaa atttaccctg ccccagggca taaagaccgg
    11401 tctggtactg cccatcctgt ggacgctgac cgagtttgcc cgcgagcgtt tcctgaccgg
    11461 attcggctgg ggcgcaatcg gctactccca aatcaccccg gacagcccgc tggcgggttt
    11521 cgcccccttg ggcggcatcc acatggttac actggcaacc gcctttctcg gtgtctggct
    11581 ggttttggcg atcgacaaca ccgcacgttc gggcaaacgc ctgcttccgg ccattctgat
    11641 tgccgccctg cttgccgcag gctacaccgc ccaacaaacc gacttcaccc gccccgacgg
    11701 cagccgcagc accgtcgccc tgcttcaggg caacatcgcc caaaccctca aatggcgtga
    11761 agaccaagtc atcccgacca tacagaaata ttacgaacag gtcggcaaaa ccactgccga
    11821 catcgtcatc ctgcccgaaa ccgccattcc catcatgcgc caaaacctgc cggaaaacat
    11881 actggcgcaa ttcgccgaac aggcgcaaaa caacggcagc gcgctcgccg tcggcatcag
    11941 ccaatacact tcggacggca acggttacga aaacgccgtc atcaacctga ccggctatca
    12001 ggaaaacaat caggacggca tcccttacta cgccaaaaac cacctcgtcc ccttcggcga
    12061 atacaaaccg ctgcccctcc tgaccacacc gctttacaaa atgatggaca tgcccctttc
    12121 cgacttccgc aaaggcggcg gcaagcaatc cgccatgatg atgaaaaacc aaaaaatcgc
    12181 cttcaacatc tgctacgaag acggattcgg cgacgaactg attgccgccg ccaaagacgc
    12241 cacactgctt gccaatgtca gcaatatggc gtggtatgga aaatccaacg ccatgtacca
    12301 gcacctccaa caatcgcagg cgcgggctat ggaactcgga cgctatatgg tccgcgccac
    12361 caacaccggc gcaaccgcca tcatctcccc caaaggcaac atcatcgccc aagcccaacc
    12421 cgatacggaa accgtattag aaggacacat caaaggctac atcggcgaaa cgccctatat
    12481 gaaaaccggc agttcatggt ggttgatggg catattgacc ctagccgcac tgattctttt
    12541 catcttccga aacaaagaac actgatacct gcacagtccg tccggacggg ctgtcttttt
    12601 tcaaaccggc cgtttacata caaccacgta tgtaatataa ttctaaaatc gaattgagct
    12661 atcaggttta caaaatcaat caaacttgcc ttacaaaatt ttcctgtctt tcaaacctta
    12721 gcgtatatct cctgccaaac atacaaaata caagccaagc agtgatatac tgcatcccaa
    12781 cacaccaaac cgccgatacg cagtttcagc cgaaaggaaa tgccccaaat gaataacgct
    12841 ttcgcattac ccgccatcca aagcggcaac ggcagcctcg aacaatacat ccacaccgtc
    12901 aacagcatcc cgatgctgtc ccaggaagaa gaaacccgcc tcgccgaacg ccggataaag
    12961 ggcgacctca acgccgccaa acaactcatc ctgtcccacc tgcgcgtcgt cgtttccatc
    13021 gcgcgcggct atgacggcta cgggctgaat caggccgacc tgattcagga aggaaatatc
    13081 ggtctgatga aggcggtcaa acgctacgag cccggtcgag gtgcacgcct gttttcattt
    13141 gccgtgcatt ggattaaagc ggaaattcac gaattcatcc tgaggaactg gcgccttgtc
    13201 cgcgttgcca ccaccaaacc gcaacgcaag ctgtttttca acctgcgcag catgcgtaaa
    13261 aaccttaatg tcctgtctcc caaagaggca caagacatcg ccgacgattt gggtgtcaaa
    13321 ctttccgaag ttctggaaat ggaacagcgc atgacgggac acgacatcgc catcatggca
    13381 gacaacagcg acgacgagga cagtttcgcc cccatcgact ggcttgccga ccacgattcc
    13441 gaaccgagcc gacaactatc gaaacaggcg cattacgccc tgcaaaccga aggcctgcaa
    13501 aacgccttgg cgcaattgga cgacaggagc cgccgcattg tggaaagccg ctggcttcaa
    13561 gacgacggcg gactgacgct gcaccagctt gccgccgaat acggcgtatc tgccgagcgc
    13621 atccgccaga ttgaagcgaa agccatgcag aaactgcgcg gtttcctgac cgaagaagcc
    13681 gaagcggttt gaaatataca caataaaata cccgacaaaa caatttgtcg ggtattttat
    13741 ttggtaaaca tcaatgcggt ttgtgcgtcg tgccgtacac gattttatcc gcccaagcca
    13801 tcgcaatcgc caaaatcaaa aagcagacat gcaccagaca ctgccacatc agctgctttt
    13861 ccgacagatt ggcggaattg ataaatgttt ggagcaaatg gatggatgaa taccgataat
    13921 cgacatcgac agccttacct tcaataccgg tgcattcaca tggctcaacc actcgggccg
    13981 gtcaggatgg tcgtcgatac gcaaccggga tacgaacgac tcatacccgc caatctgcac
    14041 catggtcagc aaattcgcaa tcataaccac atcaatcaga tttaaaacag cgagcatgat
    14101 ggcgttttcg tccgacacat ccaaattcat gaccagatgc ttcaacgatt tcaaaaactt
    14161 ataggcacaa atcgcccgta caacagtcag ccccgcataa atcggcactt gcagccaacg
    14221 gcttgcaaaa atcattttgg caaaaaaccc atagcctgtt cgggttttgg agattttgct
    14281 tgatacattt cccttccttg cagcaacaaa agcgcgtgat tccaatctac cgctgcgaaa
    14341 tcttcaattt ttcacacccg aaaacgcttg ccgccataca gatgtgatat attgattcca
    14401 ttttaacgaa cttcggagaa cgacatgagc agcggaaata tccgcatcca cagcatgacc
    14461 ggctttgcca acgcggcggc agagtgcggc agcaaacgca tcaacctcga catccgcgcc
    14521 gtcaaccacc gctttttgga cattcaaatc aggatgcccg acgatttgcg ccatttggaa
    14581 aacagcatcc gcgagcagat ttcatcccat ctggcgcgcg gcaaagtcga atgcaaaatc
    14641 caaattcaag atgcggaaaa cggcaatcag tctttggaac tgaatcgcgt tctggtcgga
    14701 cagcttgccg aaatcaacaa aaacctccgc aaacattatg atttggcaaa actgggcgtt
    14761 gctgatattt tgcgctttcc gggcgtattg gcaagccaaa aggaaaatac ggaagcattg
    14821 gcgaaaacca ttgtcgaact ggccgacaaa gcactaaaag actttaccgc cgcacgtcgg
    14881 cgtgaaggga aaaaattggg cgaacacctg ttacaacgca ttaaaaacat ggaagaaatc
    14941 atcgatgctt tgagcgaaat cttccccgcg ctggtggaag cgcataaaga aaaaatccgc
    15001 acccgccttg ccgaagccgt cggcagcatc gacaacgaca ggctgcaaca ggaatttgcc
    15061 ctctttatcc aaaaatccga catcgacgaa gaattcagcc gcctgcgcac ccacattgcc
    15121 gaagtgcgcc gtatcgttac cgaacacaaa ggcagcagcg gcaaacggtt ggacttcctg
    15181 atgcaggaac tgaaccgcga agccaacact ttgggcagca aatccatcgc taccgaatgc
    15241 acccaagcct cggtcgagct gaaagtcctg atcgaacaga tgcgcgaaca ggtgcagaat
    15301 atcgaatagc cctgcctttc tctccaaaca tgacaatgcc gtctgaaact tttttcagac
    15361 ggcattcttg atcggctgaa aacctctgtt gcaagcaatt caagattgtt ctttcacatc
    15421 caccaacctc gttccggcaa ggaagtcata cagaaactgc cggtcgggat tcagcagggc
    15481 gaacccccac ggcaaaatca gccaaaccaa tgccgcgccg gccgcgccct tgggcggaat
    15541 gccgaggaag tggcgcaatc cggcataagc aagcatagga atgaagacga tgaatatgca
    15601 cgcccaaata aagcgcaggc gcagcaggtg caaagacggc tgtatgccgt taaggtcgca
    15661 caagccgatt ttccatgtcc tcatcgccaa ggtctgcccc tgaccgtgcc agttggcgcg
    15721 gaaataaagc caccatgctc ccattatcag gatactcgtt accaatgcag aaaccgcgat
    15781 agaaacggga ttcagaaaaa tggcggcaat accggcaagc aatgctgcca aacaggtcgc
    15841 cgcaccgacc agcagcatct catacagtat ggcggcaaag cggcgtttga gcggggcggt
    15901 tttcagtatc atcatctttt caatcggaat atgccgtctg aaacgccaac agacttcaga
    15961 cggcatttgt gcaaagatgc cttgcggcgg ttaacggtgc ggacgtttct gaccgcgcga
    16021 ctgttttgcc gccatttgat tgatttcctg acgtgtcggg cgttttttga acatagcgtg
    16081 cgcctcgtcc accgaacatt tttgcgacag gcagatcagg gtaatcgcat ctttctggtg
    16141 ttgtccgccg ctgtggtaca tacggcgcag gtttgcctcg ttgattctgc ctttttcggg
    16201 atcgatgtcg aattttttca gacgttcgat aaactggcgt tgctgtcggc gggatgtcca
    16261 aacaaagccg cccgcagcgg cggcgacgat gacggcggca agcaggatgg caagcggcca
    16321 ggaatcgaca atcaatacga taatgaggct taaaacggcg gtggtcagta aaatcaggcc
    16381 gcccaagcgc agtttggtct tattgtccat agggttgatg ttcctttatt gaggtggtcg
    16441 tgccgtctga aatgttttcg gacggattgg tgaatacttt tcaggattaa cggtatttta
    16501 cagtattttc ggattttggg gattaccgct ttattccgcc agcgacacga caagctgttt
    16561 gaataccgtc catgcgtcgc cgtcttccgc gcctttgatg attcggtcga tttgggcgca
    16621 ggttttgagc gcgtcaagca ggcggacgac ggaaatccgc ttgaccgcaa gcggtgcgag
    16681 cgtctgtttg tcgccccaaa gcctgaggct gttgcggacg gattggatgc tctgcccctg
    16741 tttcagggca gcggcaagcc gaatcagcgt ccgcacgtct tcggcaaccg cccacagaag
    16801 cagcaccggt tcttcgcctt cttcctccaa cccgtccaac agcctgcata cgcgcgggac
    16861 atcgcccttc atccacgcgc ctgccagttg gaacgcgtcg aagcgggcaa cattggcaac
    16921 ggcggtttgc gcctcatcga tattgacggc atgccctttc ggatacagca gcgcgagctt
    16981 gtcgatttcc tgacgtgccg ccaacagatt gccttccacg cgctcggcaa acagtgccaa
    17041 tgcgtctgcc tcgataccca aaccgatttt gtccagccgt ccgcgtatcc attggggcaa
    17101 agccgccgca ccgaccggtt tggcttccca cacttccccc tttgccgcca atgcggcaaa
    17161 ccatttggac tggagccggg ttttctccag tttgggcagc aaaaccagcg ttaccgtatc
    17221 ttccggcagt cgggcggcaa aatcctgcaa tgcctcgccg ccgtttttgc cgggttttcc
    17281 gttggggata tgcagttcca acagtttcaa atcggcaaac agacccgcac tgcctgcggt
    17341 ttgcagcagt tcgttccaat cgaaagacgc gtcggcggta taggcttcgc ggttgagata
    17401 accctgcttc ttcgccgccg ccctcaatgc gtccactgcc tcgatacgca gcagttcttc
    17461 ctcgccgtgg atgacgtaca gaggtttcaa aggcgcgtcc gtatcgatgc gtccgatatg
    17521 tgccgccacg tcattccgcc ttcagaaagg tcaggcggcg gacaatctgt tcggcaacat
    17581 cctgccgcat ttccgcccac agggtttctt cttcttcctg tttgcccaaa atttcgttgt
    17641 cggcataatc caaaatgcgg cggacggaca cggtcatcgg tttgccgacc ggctcgccgc
    17701 gtttcaatac ctgcgcttca accgtcaata tcaaaagata ttcgttgatg actgccgcac
    17761 gggtaacggt ataggtttcc ttgttttggg aaacgctgtc tatacgcagg gtcatctgcg
    17821 cgccggcagc atcgtccacc ctgcccgaag cctgatacag cgcggtttcc aaaggaaact
    17881 gcaatgcctg tccgccttcg atgtgccagc tccggtaggt cagcggcgga gaaatgccgt
    17941 ctgcaccttt caggtggaaa ccgcacgcgc ccagcaccaa ggctgcggca gtaaggaata
    18001 ttttgttcat cgcttgcctt tctcaaaagc ataaagtcgg tagattatat cagcggcgtg
    18061 ttcagacggc attgccgtcc agccaaatca ggctcgccat acgccccgtc gctccgtcgc
    18121 ggcggtagga aaagaaaata tcccgttcca aaaccgtaca atgcgtgccg ccgtacacgc
    18181 cgcccacgcc ttcgcgcctc agaatcaggc gggcgagcgc gtagaggtcg gcaaggaatt
    18241 tgccgccgcc gataggttca aatgcagcag cggcttcggg catggacgtg cagaacgcat
    18301 caaacacgtc ctgcccgact tcaaacgcat ccgcactgat ggcagggccg agatacgcca
    18361 taatttcgac aggcggaacc ttcatcgcgg caatggtgtt ttgcaatacg ccgcccgcca
    18421 aaccgctcca gcccgcgtgt gcggcggcaa ccgccgtacc tgccctgtcg caaaatagaa
    18481 cgggcagaca gtctgcagtc atcacggcac agacggcttt acccgtgtcg tccacggaag
    18541 cgtcggcatc gggcgtgccg tccaatgctt cggcagcatt gacgacaatg gtgctgtgga
    18601 tttgattgag ataggcaacg ggcaatccga cctgctgttg cacgatttcg cggttgcggc
    18661 gcacggtttc agggtcgtcg ccgacgtgcg taccgaggtt caaactctga tacgcacctt
    18721 ggctcacgcc gccgttgcgc gtggtaatca gggttttcac attggcgggc gcgggccaat
    18781 cggcggtcag gaagtttttg cctttcgggg cgagatttag ggtttctgtg atggttttca
    18841 ttggatattg gaataggtgt gcggcggaaa gggttgatta actgccttcg gggcaaatgg
    18901 atgcttgttt tttcgtcatt cccgcacagg cgggaattcg gagattcggg attgttttca
    18961 aacgttcggg tttggattct tgccggtgcg ggaatgatgc ccttatatac tttctccgag
    19021 ctttatatgc ttcaacaggg gacggcacat caagcaccgc actgcgtgtt gcccgaacga
    19081 ggctgccctc cgattagatt ctatcgctat aaacagacgg gtttcaaccg aaaaggaatg
    19141 gggataaagt ccatttccga cacctctcgg gctataccgt ctgaaaacca atctccactt
    19201 tcagacggca ttgtttaggc gcggtcattt tgcaaattaa ggttaaggcg tgtatgcttg
    19261 ccttccctta aaaacaaaca cgcaacgcat tattatgaat atcctcagta aaatcagcag
    19321 ctttatcgga aaaacatttt ccctctgaac ctcgctcttt gccgccgccg cttttttcgc
    19381 gcccgacacc ttcaaatggg cggggcctta tattccttgg ctgttgggca ttattatgtt
    19441 cggtatgggt ttgacgctca aaccttccga cttcgatatt ttgttcaaac atcccaaagc
    19501 cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct ggctgccgtc
    19561 caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg gctgctgccc
    19621 gggcggtacg gcttccaatg tgatgaccta tctggcgcgc ggcaatgtgg ctttgtcggt
    19681 tgccgttacg tctgtttcca ccctgatttc cccattgctg actcccgcca tcttcctgat
    19741 gcttgccggt gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca tcgtcaaaat
    19801 ggttttgctc cccattgttt tgggtttgat tgtccataag gttttgggca gtaaaaccga
    19861 aaagccgacc gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga ttatcggcgc
    19921 ggtagtcggg gcaagcaaag gcaagattat ggaaagcggc ctgctgattt ttgcggttgt
    19981 cgcactccgc aacggcatcg gctacctgct cggcttcttt gccgccaaat ggaccggcct
    20041 gccttatgat gcacaaaaaa cgctggccat cgaagtcggt atgcaaaact cgggcctggc
    20101 cgccgcgctt gccgccgcac actttgccgc cgcgccggtc gttgccgttc cgggcgcatt
    20161 gttcagcgtg tggcacaata tctccggctc gctgctggca acttattggg cggccaaagc
    20221 cggtaaacat aaaaaaaccc taaacgcctt tcatcataat gccgtctgaa cggggcaaac
    20281 gaaaccggcg gtttctgccc ggccccttca gacggccttt gtttgaaatc ggcggggacg
    20341 attgcccggc tgctccggtt cggctgcctg cgttcgcccc gcctttcaag ccgcctcact
    20401 cccttacata aaccacttcg acatcgtagt cgtcttcgtt ccaatcgtca tcgtcgtccg
    20461 cgccgaattt gtcctgccat tcctcttcat tgctcaaaga cgaatccaaa ccggcttcaa
    20521 gacggaggac ggacaacaaa tgatatatgt cgtccggaat cggtgcctca aacgaaacgg
    20581 tttcgccgct ttccggatgg gtaaaactca agcggtaggc gtgcaacgcc tgacgcgcgc
    20641 ccaaactttt aacggcttct ttcaccgtgt cgccgcacgg atggcgcggg ttgccgtaaa
    20701 ccgggtcgcc ggcaagcgga tggttggcct cgcgcatatg gacgcggatt tggtgcgtcc
    20761 tgcccgttcc gagcgagcat tcgatgtagc tgtgggcaag atagcgttcc aacactttga
    20821 cgtgggttac ggcaggcttg ccgccgaatt tgacggctgc cattttcagg cggttgtgcg
    20881 gatcgcgtcc gatttgggtt tcgattttac cgtcaaaggg gacgatgccg ttggcgacgg
    20941 cgcggtagat gcgtttgacc gtgcgttctt gaagctgcct cacgagggaa ttttgcgccg
    21001 gcagggtttt ggcaaccacc atcagcccgc tggtttcttt gtccaaacgg tgtacgatgc
    21061 ccgcgcgcgg aatttgggac agctcggggc aatgcgccaa caggccgttg agcagcgtcc
    21121 ccgtccagtt gcccgccgcc ggatgcacca ccagtccggc cggtttgttg acgacgatga
    21181 cggtatcgtc ttcgtaaaca atatccaaat ccataggctc cggaacaaac gccagatttt
    21241 cctcactcgg acggacggtt acacaaattt gctcgccgcc tatcattttg tctttgggtt
    21301 gcgaaggttt atcgtttaca ataaccgcgc cttctttaat ccatgatgtc aggcggctgc
    21361 gcgaatagtc gggcagaagt ttcgccaaca ccgcatccag ccgcccgccg gcaagctcgg
    21421 gcggaacggt cagaccgata cggttttcag ttccggaagc tgacgcaaag tctgaatcgt
    21481 cgctataatc ggattcatta tcaaaggaag tattctgcat gaaaaaaatt cttttaacgg
    21541 tttcattagg tttggcactg agtgcctgtg ccactcaagg tacggccgat aaagatgccc
    21601 agattaccca agattggagt gtggagaagc tctatgccga agcccaggac gaattgaaca
    21661 gcagcaatta tacgcgggct gtcaagttat acgaaatctt ggaatcgcgc ttccccacca
    21721 gccgccatgc ccggcaatcc caactggata ccgcatacgc ctattataaa gacgatgaaa
    21781 aagacaaggc tctggcggca atcgaacgct tccgccgcct ccatccgcag catccgaaca
    21841 tggattacgc gctgtacctg cgcggcttgg tgctgtttaa cgaagatcag tccttcctga
    21901 acaaactggc ctcgcaagac tggtccgacc gcgacccgaa agccaaccgc gaagcgtacc
    21961 aggcgtttgc ggaactcgtc caacgcttcc cgaacagcaa atacgccgcc gatgcgaccg
    22021 cacgcatggt caaactggtc gatgctttgg gcggcaatga aatgtcggtg gcgcgttact
    22081 acatgaaacg cggcgcatat atcgccgccg ccaaccgcgc caaaaaaatt atcggcagct
    22141 atcaaaatac acgctatgtc gaagaatcac tggccatatt ggaacttgcc tacaagaagt
    22201 tggacaagcc ccagcttgcc gccgatacgc gccgcgtatt ggaaaccaac ttcccgaaaa
    22261 gcccgttttt gacgcacgct tggcagcccg acgatatgcc ctggtggcgt tactggcatt
    22321 aagcccatcc tcgaaatgcc gtctgaagcc ctttcagacg gcattttccg ttccggcacc
    22381 agccttcccg ttcactcaaa cggttttttc tgccaataga atttatacac tctcaaacgt
    22441 tgagccttta cgcctccgcg tcccaagccg aattgcagcg cgcccgacaa atccgtccgc
    22501 aacaacttga tgccgtgtgc gcggacacgg ttctgcaccg cttcggtcgg atgcttgtag
    22561 gcgttggcat aaccgcttga agcaacggca tattcgggcg aaacggcatt gaggaacacg
    22621 cccgacgagg acgtattgct gccgtgatgc cccaacacca acacctggct gtacaggttg
    22681 cctccatact tgccgaccag gctttcctcg cccttcgtat ccaaatcgcc cgttaccagc
    22741 agtgccgcac cgcccgccac aacacgcaaa acacaacttt tcccattatc atcgatattt
    22801 ttgcgttcag acggcctcaa aaactcgaaa tccacgccgt cccattgcca acgctgttcc
    22861 gcacaatgcc gcgccccctc ataaaattcc ggctgtccgg cataaatccc gccgttcggt
    22921 atcttgccga ctgcctgaaa gccgccgtcg tggtcgctgt cgtgatgcga cagaaccagc
    22981 ttgtccaaac ggcggacacc cgccgcattc aaactcggca cgatacccgt ttgtgccgcc
    23041 gctaccgttc ccgtatcgaa aagcagatgg cggtttgccg tccgcacaag caccgacaag
    23101 ccctgccccg catcccaaac cgtaaccgca gcttcgtttt caggtactgc ttcaggacgg
    23161 taggatacaa accctgccaa cagcaacacc gcccacggac gcaaacccaa gccgcgcggc
    23221 agcagcaaca gcaaagcggc acacaccgcc aacaccagca gcggcaaagg tgcggcggca
    23281 acggcaaact caggcgacac atcggcaagc cacaccaaaa accgcaaagt gtattccgcc
    23341 aaaaatgccc ccgcctgctg aagcggcgca aacggcacga ccgaacccag caacgccagc
    23401 ggggtcaaca cccaagaaaa ccacggtatc gacaccgcat tgaccaaagg gctgaccaaa
    23461 ggcagcgagg caaacagata accgagcaaa accagagaca acaccgaagc cgcccactgc
    23521 cggcgcacgg cggtttgccg tttcccctcg tacaaacgcc ctgcacacgc ccatatcagg
    23581 gccgccacca aaccgaaaga cagccaagta ccgacaccca aaaccgccaa agggtcgaac
    23641 agcagcaccg ccgccaacgc ctgccaccac gtcgcccacg ccgacagtct tcccctgcgc
    23701 cacgcccacg cgaacgccgc caacatcaaa acactgcgct gcgtcggcac ggaaaaaccg
    23761 gcaagcagcg cgtaaaacag cgcgcccgca caccccgccg ccaaaaccca cgcgcgcggc
    23821 cgggcgggaa ggcgcgggga acaggcaagc agccgcttcg ccagccacgc aaacagcacc
    23881 gccaccatcg taacgtgcaa acccgagata ctgaccaaat gcgtcagtcc caacggccgg
    23941 aacgcctgcc acaattcggg gcgcaatgcc gactgttcgc ccacgctcaa cgcgcgcatc
    24001 agcccgatgc cgtctgaaag cccgccgtcc gcatccgcct gccgccaatt gcggctgatg
    24061 cggctgcgcc aaaccgcaat cccccaaccg cttccgtcat gcagcaaaac cctgtccgca
    24121 ccgaccgtcc ccacgccgcc tatcccgttg gataatgccc acgcctcacg gttcaaaccg
    24181 cgcaggttca actccccgac gacggggtgc acgcgtgccg ttatccgcca tctgctcccg
    24241 accgcccatt cgcgccgttt gtagtccgac agcagcaaat caaacgtccg accgccgctg
    24301 tccacagcct ttgccgcaaa ctgcacgcgc cgcccgtccg acctcggcat atccgccact
    24361 tccaccgtca aaggcacgcc tgaaaccgcc tccgcccgcc attgcgaaga cagtgccgct
    24421 tccgttctga atacgccgta agccgcgccc gccaacacgc acagcatcag accggcaaac
    24481 gcaaaacgcc ttgcaagcac agccaaaacc gcaaaagccg ccaaccaaaa cggccaatgc
    24541 ggtacaaccg acagtgcgaa cgaaactgcc acgccgacaa cccaacacgg caaaaaccaa
    24601 cgacggaaca cgtctcccca ctcccgatat aaaaaacaaa cgatgataaa gcaaagccat
    24661 aaaaccgtca taacaagatt tgaaaatgat aagaatgcaa acgaaaagcc tcagtattat
    24721 ccgagacaac ggcaaacttt ccatcgtcat tcccgcaaaa gcgggaatcc aaagacaaaa
    24781 agccgcagaa atttatcgga aagaaccgaa ccacaaaaac cggcttccca ccttcgcttg
    24841 aataacagaa caaagaaagc ggaaataaaa acatcgggat tcttttttaa attggcaata
    24901 aaacaccaaa atgaatgagt tacacaaaaa aatactcaac accacccaac cggcgtaaaa
    24961 tgcaaaacat tatcgctatt aaaacggtaa aaccttatga aagccaaacg ttttaaaatt
    25021 aacgccatat ccttatccat ctttcttgcc tatgccctta cgccatactc agaagcggca
    25081 ttggtgagag acgatgtcga ttatcaaata ttccgtgact ttgcagaaaa caaaggcaaa
    25141 ttttttgtcg gcgcaaccga tttatcagtg aaaaacaaac aaggtcaaaa catcggcaac
    25201 gcattatcca acgtaccgat gattgatttt agcgttgcag atgtcaacaa acgcatagca
    25261 acggtagtcg atccccaata tgccgtcagc gtcaaacacg ccaaagcaga agtccatact
    25321 ttttattacg gccaatacaa cggtcataat gatgtagccg acaaagaaaa tgaataccgc
    25381 gttgtcgaac aaaataacta tgaaccccat aaagcttggg gtgcgagcaa tttaggccgc
    25441 ctcgaagatt ataatatggc gcgttttaat aagtttgtaa ccgaagttgc gccgattgca
    25501 ccaactgatg ccggcggcgg attagatact tacaaagata aaaaccgttt ttcctctttt
    25561 gtcagaatcg gtgcgggcag gcaattagtt tatgaaaaag gggtttatca tcaagaagga
    25621 aatgaaaaag gctacgattt gcgcgatctt tcacaagcct atcgttatgc cattgcaggt
    25681 acgccttata aagatattaa tattgaccaa acaatgaata ccgaaggttt gatcggcttt
    25741 ggtaatcata ataaacagta ttcggcagaa gaacttaaac aagcactttc gcaagatgcg
    25801 ttaacaaatt acggcgtgtt gggcgatagc ggctctccgt tatttgcttt tgacaaacaa
    25861 aaaaatcaat gggtcttttt gggaacttac gattattggg caggttacgg aaaaaaatca
    25921 tggcaagaat ggaatatcta taaaaaagaa tttgcagata aaatcaaaca acacgataac
    25981 gccggcaccg tcaaaggtaa tggagaacat cattggaaaa ccacgggtac aaacagccat
    26041 atcggttcga cagcggtaag gcttgccaac aatgaaggag atgcgaacaa cggacaaaat
    26101 gttacctttg aagacaacgg cactttggta ttggatcaaa acatcaacca aggcgcgggc
    26161 ggcctgtttt tcaaaggcga ttacacagtc aaaggcgcaa ataatgacat cacttggtta
    26221 ggtgcgggga ttgatgttgc cgacggcaaa aaagtcgttt ggcaagtcaa aaatccgaat
    26281 ggcgacagat tggcaaaaat cggcaaaggc actttggaaa taaacggcac aggcgttaac
    26341 caagggcaat taaaggtcgg cgacggtacg gttattctga atcaaaaagc cgattcaaac
    26401 caaaaagttc aggctttctc ccaagtcggc attgtcagcg gacgcggtac attggtgctg
    26461 aacagtccgg accagattaa cccgaataat ctgtatttcg gtttccgtgg cggtcgtttg
    26521 gatgccaatg gcaatgactt gacttttgaa cacatccgca acgtggatga aggcgcgcgc
    26581 attgtcaacc acaacacaga ccacgcctcc acaatcacac taacgggtaa atctttaatt
    26641 accaatccga acagcctttc ggtacatagt atacaaaatg actatgacga agataattat
    26701 agttattatt accggccaag gcgacccatc ccacaaggca aagatcttta ttacaaaaac
    26761 tatcgttatt acgccctaaa atccggtggc agtgttaacg caccgatgcc cgaaaacgga
    26821 caaacggaaa ataacgactg gattttaatg ggcagcacac aagaagaagc caagaaaaac
    26881 gcgatgaacc ataaaaacaa tcagcgtatt agcggtttta gcggtttctt tggcgaagaa
    26941 aacgaaaaag gacataacgg ggcattaaac cttaatttca atggcaaaag cgcgcaaaac
    27001 cgtttcttgt taacaggcgg cgcgaattta aacggaaaaa taagcgtaac tcaaggcaat
    27061 gtcttgttat caggtcgtcc aacaccacac gcaagagatt ttgtgaacaa atcttcagcc
    27121 cgaaaagacg cacatttctc caaaaacaat gaagtcgtat ttgaagacga ctggataaac
    27181 cgcacattca aagccgcaga aattgcggtt aaccaatccg catcattctc ttccggaaga
    27241 aatgtatccg acatcaccgc caacatcacc gcaacagaca acgccaaagt aaatttgggt
    27301 tacaaaaacg gcgatgaggt ttgcgtgcgc tcggactata ccggttacgt tacctgcaac
    27361 acaggcaact tatccgataa ggctttaaac agctttgatg cgacacggat taacggcaat
    27421 gtgaatttga atcagaatgc ggcattggtc ttgggcaagg ctgcattatg ggggaaaatt
    27481 caaggacaag gaaacagccg tgtcagccta aaccaacata gcaaatggca tttgaccggc
    27541 gacagccaag tacacaatct gtcattggcg gatagccata ttcatttgaa caacgcttcc
    27601 gatgcgcaaa gtgcaaataa ataccacacg atcaaaatca atcatttatc cggtaacggg
    27661 cattttcatt atctgacgga cttggcgaaa aatcttgggg ataaagtgct tgtgaagaaa
    27721 tccgcatccg gccattatca gctccatgtt caaaataaaa caggcgaacc taatcaggaa
    27781 aggctggatc tctttgatgc atcatccgta caagaccgct cccgcctttt tgtttccttg
    27841 gcaaatcatt acgtcgattt aggcgcattg cgttatacaa tcaaaacaga aaacggtatt
    27901 acccggttgt acaatcctta tgccgggaac cgccgcccag tcaagccggc cccgtctcct
    27961 gccgcaaaca cggcttctca agcacaaaag gcaacacaaa cggacggtgc acaaattgcc
    28021 aagcctcaaa atatcgtcgt cgcaccgccc agcccgcagg caaatcaagc cgaagaagcc
    28081 ctccgccaac aagcaaaagc ggagcaagtg aagcgtcagc aagcagaagc agggcgtaaa
    28141 tcggcagagt tgtcggcaaa acaaagggcc ggggaagagg aacgccgtca aacagcccaa
    28201 tcccagccgc aacgccgcaa acgccgtgcc gcaccgcagg attatatggc agtttcccaa
    28261 gaccgtccga aacgccgcgg acaccgttcg gtacagcaaa acaatgtgga aattgcccaa
    28321 gcccaagcag aacttgcgcg ccgacagcag gaagaacgca aagcggcaga gttgttggca
    28381 aaacaaaggg cagaagccga acgtgaggct caagcattgg ctgcgcgccg aaaagccgaa
    28441 gccgaagagg caaaacacca agctgccgaa cttgcacatc gccaggaagc aaaacgcaaa
    28501 gcggcagaat ccgcgaaacg aaaagctgag gaagaagagc accgtcaaac agcccaatcc
    28561 cagccgcaac gccgcaaacg ccgtgccgca ccgcaggatt atatggcagt ttcccaagac
    28621 cgtccgaaac gtcgcggacg cagatctact ctgccggcac cgccctcgcc atcatttgat
    28681 tcatcagctt acgcagcacc cagggccttg cataatccgg actggtatga aaatgattat
    28741 gaaggaatcc ccttggatgc gttggaagat gaagatgtat ccgaatcggt tgacacatca
    28801 gacaaacagc ctcaagacaa tacggaactt catgaaaaag ttgaggcagt gagtttgcaa
    28861 ccaagagccg cgcagccgcg aacccaagcc gccgcgcaag ccgatgcagt cagcaccaat
    28921 actaactcgg ctttatctga cacaatggca agcacgcaat ctatcttgtt ggatacaggt
    28981 gcttcattaa cacggcacat tgcacaaaaa tcacgcgctg atgccgaaaa aaacagtgtt
    29041 tggatgtcaa acaccggtta tggccgtgat tatgcttccg cacaatatcg ccggtttagt
    29101 tcgaaacgca cgcaaacaca aatcggcatt gaccgcagct tgtccgaaaa tatgcagata
    29161 ggcggagtat tgacttactc tgacagtcag catacttttg atcaggcggg cggcaaaaat
    29221 acttttgtgc aagccaacct ttatggtaag tattatttaa atgatgcttg gtatgtggcc
    29281 ggcgatattg gtgcgggcag cttgagaagc cggttacaaa cgcagcaaaa agcaaacttt
    29341 aaccgaacaa gcatccaaac cggccttact ttgggcaata cgctgaaaat caatcaattc
    29401 gagattgtcc ctagtgcggg tatccgttac agccgcctgt catctgcaga ttacaagttg
    29461 ggtgacgaca gtgttaaagt aagttctatg gcagtgaaaa cactaacggc cggactggat
    29521 tttgcttatc ggtttaaagt cggcaacctt accgtaaaac ccttgttatc tgcagcttac
    29581 tttgccaatt atggcaaagg cggcgtgaat gtgggcggta aatccttcgc ctataaagca
    29641 gataatcaac agcaatattc agcaggcgcc gcgttactgt accgtaatgt tacattaaac
    29701 gtaaatggca gtattacaaa aggaaaacaa ttggaaaaac aaaaatccgg acaaattaaa
    29761 atacagattc gtttctaaaa tactaaattc atagcaaaat aaaatgccgt ctgaactcaa
    29821 gcttcggacg gcatttttat cagactaaca aagctacagc tcaatgcctt tgagtttcgc
    29881 cacggtattg atgtctttgt cgccgcgacc cgacaggttg accaaaatca cttggtcttt
    29941 acccattttc ggcgcgtttt tcaccgccca agcaacggcg tggctggatt cgagcgcggg
    30001 gatgatgccc tcgaatcggc agagcaagtc aaaggcttcg agtgcttcgt catctttggc
    30061 aacggtgtat tcgacgcgct tgatgtcgtg cagatggctg tgttccgggc cgatgccggg
    30121 gtaatccaag cctgcggaaa cagagtgcgt ccccaagact tggccgtttt cgtcctgcat
    30181 cagatagctg cggaagccgt gcagtacgcc aatcggtgcg cccgaagtaa tcggcgcggc
    30241 gtggtcaggc gtatccacgc ccaaaccgcc tgcttctacg ccgaccaaac gtacgttttc
    30301 ttcttcgata tagggataga acaaaccgat ggcgttcgag ccgccgccga cgcaggcaac
    30361 ggcgacgtcg ggctgtctgc cggtggcttc ctgcatctgc gctttggctt cgttgccgat
    30421 gacgcattgg aaatcacgca ccatttcggg atacggcgcg gggccggcgg cggtgccgat
    30481 gatgtagaac gtatcgtcca ctcgggcgac ccattcgcgc atcgcttcgt tcatcgcgtc
    30541 tttcaaagta cggctgccac tgtcaacgcc gatgacattc gcaccaagca gcttcatacg
    30601 gaacacattc ggcatttggc gttgaatatc gtccgcaccc atgtacacgt cgcaagtcat
    30661 gccgaaacgt gcggcgacgg tggcagaagc cacgccgtgc tgacccgcgc cggtttcggc
    30721 gatgacgcgt tttttgccca tacggcgggc aagcaatgcc tgaccgatgg tgttgttgac
    30781 cttgtgcgcg ccggtgtggt tcaagtcttc gcgcttcaac cagatttgcg cgccgcccaa
    30841 atgctcggac aaccgcgcgg cgtggtaaac ggggctgggc ctgccgacat aatgtttcaa
    30901 atcgcggcgg aactccgccc aaaattcagg atcgtttttc gcttcgttgt aggcctgttc
    30961 cagctctttc aaagcgggaa tcagggtttc ggaaacataa agcccgccgt gttcgccgaa
    31021 aaagcccttc tcgtcgggcg cgtggtagtt cttcatcgga ttcttcctta tgcttcaggt
    31081 tttcagacgg catttccgtt ttttcaaaca tgccgtctga acgggaaatt gtcggcaatt
    31141 ataactgctt ttcacacaag cccgccattt cgaaaaacgc ccgatacgcc gctgcctttt
    31201 tcgccaaact caaaggatag gcgcgcgaag tcgaaggcag gcgcgtcagt gtcaaatccc
    31261 gtccggcaaa cggaaacggc acggtttcgc ccgttttcgg cattttgatg ccgccgccct
    31321 gaatatcgag caggatttcc gtcgccttgc cgcctgtcgt acaaatatgg cggcagtctg
    31381 gtattttcgc caacaccgcc gccaaatcga cggtttcaac tacctttaaa aacttgtcgg
    31441 acgcattgcc gtgctccctt gccgccttca acacggtcgg acaggacgca atcccccgtt
    31501 cgtgcaaaaa cgccttgatt ttctccgcgt caaacgcttt ttcagagtgc gcagcctccc
    31561 cgtgggacgg catccggaaa tgcgccgcat cgttaaaaaa caccagcccg taaacgcgcc
    31621 acatatcgtt ttggaaattc ggataatgaa actgcatcgc gcgtttgtct tccttgggcg
    31681 gaaacgtccc catcatcatg accgcagcct tcggcggcaa cacgggatcg aagggatggg
    31741 tttcgatttc aagcagattt tcagacacgt tcaatcctcc aacaggcaaa gcggcagaca
    31801 gccggtttgc accaaaccgc caaacacggc aagccttcaa acagcattat cacgccctgc
    31861 cgttttcggt tacaatgccg acatcaaacg gatactgtaa acacatttat gttccaacac
    31921 acaggacgac acataaagca ccgccctatg tgtcgtcctg atttggaagg ggttacgccc
    31981 cgcccaaaca gagtctgatc ctgccgccct aaagggcggg gtttcaaccg aagaggaaac
    32041 acgatgaaag aacacaaagc ccgcaagcgt ttcgggcaga attttttgca ggacacgcgg
    32101 attatcggcg atattgtcaa cgccgtgcgc ccgcaggcgg atgatgtcgt gattgaaatc
    32161 ggcccgggtt tggcggcaat taccgaacct ttggcgaaaa agctgaaccg cctgcacgtt
    32221 gtcgaaatcg accgcgacat cgtatgccgt ctgaaaacgc tgccgtttgc ggataaactg
    32281 gtgattcacg aaggcgacgt gttgcagttt gatttcaacg gcatctcagg caaaaagaaa
    32341 atcgtcggca acctgccgta taacatctcc acgccgcttt tgttcaaact ggcggaggtg
    32401 gcggacgatg tcgccgatat gcactttatg ttgcaaaaag aagtggtcga gcgcatggtt
    32461 gccgcgccga aaagcaacga ctacggtcgc ttgggtgtga tgctgcaata ttttttcgat
    32521 atggagctgc tgattgacgt gccgcccgaa tcgttcgatc ccgcgccgaa aatcgattcc
    32581 gcagtggtgc gcatgattcc ggtgaaacac cgtatcggca aggcggacga tttcgagcat
    32641 ttcgccaaac tggtgaaact cgccttccgc caacgccgca aaaccatacg caacaatctg
    32701 aaagagcttg ccgacgacga tgatttgcag gcagtcggca tcagtccgca agaccgtgcc
    32761 gaacacatcg cgccggagaa atatgtggcg ttgagcaatt atctggcgga caaggccgtc
    32821 tgaaaaggga cgcggcgatg gaaagcaacc tgcagaaaac cgagcgtttg atccgcgaaa
    32881 tcaaccgcct gcacgcacaa tattcgcagg attattttga aaccggcaaa gtgcggaaaa
    32941 tcaatttgag ccacacgctc aaaaacgttc cgaccgaaca tattttgtct taccgcctta
    33001 atttgcacga agcagttaac gattatttag cctttgccga tacgcgcggc atagactttt
    33061 tctaccgcgt gaaaaccgca gaaagcatac atgataaaat caaccgctat ctggcacgcg
    33121 gcacccaata tccgaccaac aacatcctga acgatatttt cggcgcgcgg ctgatttggc
    33181 cgtctgaaac agtcgccggg attttagaaa aacttgacgg ctggaaaaca gaatacggct
    33241 tgaaaaactg gtatttgcgc gatgccggcg gctatatcgg catacacatt tatttcaaaa
    33301 acagcagcaa tttctactat ccgtgggaat tgcaggtttg ggatgaaaaa gatgccaaag
    33361 ccaatattga aaaccatatg gcgtacaaac ggaatttcgt gcgttgaatt gccggcaagc
    33421 cgccttgaaa ccgtttataa ctgtttataa gagaataaac cataaaatac acaaccgtat
    33481 tcctgacttt tcctttaacc gcccgagtga acaaactatg aaatacacag ccgcactcct
    33541 gacttttctt ttaactgcct gcatgaaccc gaacgatgcg tttttccaaa accggcgtta
    33601 tcaaatgccg gaggcgcaat tgaacggttc aaacgccgtt ttccattacg gttattccca
    33661 aaatcccgat catgatttat tggtcgacca agtccgcgtg ccggacgcgt acagggcttc
    33721 atttgcgcgg gattggatag cgaaggagca aggttaccgc aaaaacgact gcgtactgga
    33781 aaaagcggaa agcgggggtg tgacgtacta cacctgcgat gcggaaaaag gattcaacgg
    33841 cacacgtttc gtcatctatg tcgtgaatat gaaaaacgac aaaggctacg tcaaagttta
    33901 ccggggcatg aataagccga atcaggaaga gttaaccaaa ttggttgccg atttgaataa
    33961 gttttaccct taatcagcca gacggattca gactgcccga aaagaatgga cactatgatc
    34021 aaattcaaaa acgtacacaa acatttcaaa gacctgcacg tcatcaacgg cgtaaacttg
    34081 gaaatcaaaa aaggcgaagt ggtcgtcgtc tgcgggcctt cgggcagcgg caaatccaca
    34141 ctcatccgca ccgtcaacca gcttgaaagc atcgaaagcg gcgagatttg ggtggacggc
    34201 gtcaacgttg ccgaccccaa aaccgatttg aacaaaatcc gcgaagaagt cggctttgtg
    34261 tttcaaagtt tcaacctcta cccgcacctg accgtgttgg acaacatcac cctcgcaccg
    34321 atgaaggtta aggggcaaaa tgcggaacag gcggagaaaa aggcaatgga gcttttggaa
    34381 cgcgtcggac tggcacacaa aaaagacgcc ttcccttccc aactttccgg cggccagcag
    34441 caacgcgtgg cgattgcgcg cgggctggcg atggagccgc gcgtgatgct gtttgacgag
    34501 ccgacttccg cactcgaccc cgaaatggtc ggcgaagtgt tgaaagtgat gaaagacttg
    34561 gcggaaagcg gcatgacgat gatgtgcgta acccacgaaa tggggtttgc ccgcgaagtt
    34621 gccgaccgcg tgattttcgt cgataaaggg caaatccttg aagacgaaac gccggaagcg
    34681 tttttcacca acccgaaaca cgaacgcgcc aagcagttcc tgcaacaagt catgacccat
    34741 tgatgctatg ccgtctgaaa accgaacgtt cagacggcat agcccgacac cgcaagatga
    34801 ttgagaaagc acaccatgta ttttgttgac cgcaccgccg ccgtcctcaa gccgaccgcg
    34861 cgctttttgg aatggctcaa aagcaccgac gaaaatatgc ccgacctgac catagaacag
    34921 cttcgcgccg actgttccgt cttcctcgtt ccgcagttcg acgaaccgga agccgtcgtt
    34981 tcctacttcg acgaacgcta ccggcagatt tttgaggcgg agctggcagg ttgggacatc
    35041 gacaaggaca aatggccgca agatatgggg ctgaaggcgt tttgggaatt tttcgacatt
    35101 gaaatccacg atatggtttt ggatatggaa gaagcggatt tgaacatcac gcccgtgttc
    35161 gacaatatga tgtaacgcca tgcgcgcctt tcagacggca ttgaggccgt cccgcatcct
    35221 gaacatcctg accgtatcac tccactcggc ttccattgcc gtctgcctga cttggtttta
    35281 cggccggatg atgtggttcg gtttggcggc tttggcggca agttacgcat actcgctcag
    35341 gataacgaat ctgaaacacc gccatgccat aaccgccatt accatagacc gcgacggacg
    35401 ggcggaaatc gtatccggca aagacaaaac agcggcaaca cttgcgggca gcagtatggt
    35461 tacgccttat gccttattct tacaatggga cacaggcggc aaaaccgtcc gccaatgcat
    35521 catgcccgac atgacggaca aagaatccta ccggcggctg aaagtctggg tgctttggcg
    35581 gcagccgaaa aaaaccgccg aaaccgatac atcagactga tttcagacgg catttgaaaa
    35641 cgaaacactc atgaaaacat tacaagactg gctctcgcat ttggaaaccg cccacagcgg
    35701 cggtttgatc gacatgggtt tggagcgcgt aagcgaagta aaaaagcgca tgaacctgac
    35761 accgcaatgc cccgtcgtcg tcgtcgcggg aacaaacggc aaaggttcgg tctgcgccta
    35821 cctgacacag atttacaaac aggcaggctt caaaaccggc acgctgacca gcccgcattt
    35881 attgcattac aacgaacgca tcgccatcaa cgccgaaccc gtttcggacg atacgattat
    35941 tgcctctttc gagcgcatcg aagccgcgcg cggcgaaata tcgctgactt attttgaatt
    36001 caatgccttg gcggctgtcg acattttcat acgcgaacaa gtggatgtaa tgatattgga
    36061 agtcggcttg ggcggacgtt tggacgcggt caacgcgttt gacggcgatt gcgcggtggt
    36121 tactagtgtg gatttggacc atcaggcatt tttgggcgat acggtcgaac aggtcggctt
    36181 tgaaaaggca ggcgtgttcc gcagcggcaa acccgccatc tgcggtcaaa accccgcgcc
    36241 cgcatcattg gtcgcacacg ccgaagccat aggcgcgaaa ctgctgatgg tacagcgcga
    36301 tttcgagttt cacgcgatgg aaaacatcca atggaactac cgcttccggc cgcagcattc
    36361 agacggtccc gcacgcaacc gcaatgccct gcccttcccc gcattgcgcg gcgcatacca
    36421 gctttctaac gccgcctgcg cgttgaccgt attggaatgc ctcgacgaca gactgccggt
    36481 ggacattggt gcaatcaaac gcgggctgct gctggttgaa aatcccggac gcttccaagt
    36541 cctgcccggc cggccgctga ccgtattgga tgtcggacac aacccccacg ccgcccgcgc
    36601 cctgcgccgc aacctaatca accttgccta cgcgcaaaag cgcaccgccg tgttcagtat
    36661 gctgtccgac aaagacatag acggcgtgtc ggaaaccgtt aaagaccagt ttgacgagtg
    36721 gtatatcgcg cctttggacg tgccgcgcgg tatgacggcg gatgcactga aagccaaatt
    36781 ggaacaacac cacatcgaaa acatacagac attcgccgcc gtccgcgacg cgtaccgggc
    36841 ggcagcctca aaagcgggcg aagacgacag aatcgtcgta ttcggctcat ttcataccgt
    36901 cgccgacgta atgtcggtac tgtgaaaagg aaacttctat gtccgaaaac aaacaaaacg
    36961 aagtcctgac cggttacgaa cagctgaaac ggcgcaaccg ccgccgcctc gtaacggcaa
    37021 gctccctggt tgccgcctcc tgcatcctgc tggcagccgc actcagttcc gatcctgccg
    37081 acagcaatcc cgcaccgcag gccggcgaaa ccggcgcaac ggaaagccaa acggcaaaca
    37141 cggcacaaac ccctgccttg aaatccgccg ccgaaaacgg ggaaaccgcc gccgacaaac
    37201 cgcaggactt ggcaggcgaa gacaagcctt ctgccgccga cagcgaaatc agcgagcctg
    37261 aaaacgtagg cgcgccgctg gtgctgatta acgaccggct cgaagacagc aacatcaaag
    37321 gtttggaaga atccgagaaa ctgcaacagg cagaaaccgc caaaaccgaa ccgaagcagg
    37381 caaaacaacg cgctgccgaa aaagtgtcgg caactgccga cagtacggat acggtagcgg
    37441 ttgaaaaacc gaaacgcact gccgaaccca aaccgcaaaa agcggaacgc actgccgaag
    37501 ccaagcccaa agccaaagaa accaaaaccg ccgaaaaagt tgccgacaaa ccgaaaactg
    37561 ctgccgaaaa aaccaaaccg gatacggcaa aatccgacag cgcggtaaaa gaagcgaaaa
    37621 aagccgacaa ggctgaaggc aaaaagacag ccgaaaaaga ccgttcggac ggtaaaaaac
    37681 acgaaacggc gcaaaaaacc gacaaagcgg acaaaaccaa aaccgccgag aaggaaaaat
    37741 ccggcaaggc gggcaaaaaa gccgccattc aggcaggtta tgccgaaaaa gaacgcgcct
    37801 tgagcctcca gcgcaaaatg aaggcggcgg gtatcgattc gaccatcacc gaaatcatga
    37861 ccgacaacgg caaagtttac cgcgtcaaat caagcaacta taaaaacgca agggatgccg
    37921 aacgcgattt gaacaaactg cgcgtgcacg gcatcgccgg ccaggtaacg aatgaatagc
    37981 ctccccattg ccgacctcct cgcctccgcc gtcatcgccg cctgcatcgt catttccacg
    38041 atgcgcggcg tgattgcgga agcaggttcg atggtggcat gggtggtttc cttctttttt
    38101 gccaaactct ttgccgcacc cttcgccgac ctcgcctttg cctcgttcca accccgcctg
    38161 tttgcattgg ctctgtcatt catttccctg ttcgtcattg cctgtctgat ccagaaaatg
    38221 ctccgttcgc tgctgaccgg cgcagtttcg gcggtcggtc tgggctttgc caaccgcatt
    38281 ttgggcggtg tattcggtgc attgaaaggc gttttgattg ttaccctgct gatcatgctt
    38341 gcttcaaaaa ccgacctgcc cgataccgaa gaatggcaac agtcctatac cgtaccgttt
    38401 ttcgtatcgc tttccgaagc ggtgttaaac catacggaca acgcacccga atccctcgac
    38461 gacgactaat ccgcatacgt ggcgtttcag acagcatacc tgatttatgc cgtccgaaac
    38521 gccttttctt tccttgacac accccgacag acaaagcgca cctgcctgac tttacacaaa
    38581 tattgtcaac aattcgggca aaaagccgcc gtttgcttta taattccgtt tcacccttaa
    38641 acctcgaatc ggagaagacg atatgtgcgg cgtattaggt ttggtcagtc atgaacccgt
    38701 gaaccagctt ctgtacgacg gtttgcagat gttgcagcac agggggcagg atgcggcggg
    38761 cattgcaacg gcggaaggcg gtaccttcca tatgcacaaa ggcaaaggga tggtgagcga
    38821 agtgttccgc acgcgcaata tgcgcgattt gaccggcaac gccggcatcg cccacgtccg
    38881 ttatcccacg gcgggcaacg ccggcagcag cgcggaggcg cagcctttct acgtcagctc
    38941 gcctttcggc atcgttttgg cgcacaacgg caacctcacc aacactgccg aactgtatga
    39001 aaacgtgtgt aacaaacacc tgcgccacat caacaccggc tccgattccg aagttttgct
    39061 caacgtattc gcgcacgaat tgcgccgcga agtctctaaa aacgccgacc cgcaccggct
    39121 caatgccgac aatattttca acgccgttgc cgaagtccac cgcctggtgc gcggtgcata
    39181 cggcgtggtt gccatgattg cgggctacgg tatgctcgcc ttccgcgacc cttacggcat
    39241 ccgcccgctg gcattgggtt cgcaaaccga cagcgaaggc aggaaatcct atgccgtcgc
    39301 ctccgaatcc gtcgccttca atgcgcttgc ctacgatttg gaacgcgata tccggccggg
    39361 cgaagcggta tttgtcggct ttaacggcac aatcatcgcc cgacaatgca gcgaccgcgc
    39421 caaactcagc ccctgccttt ttgaatatgt ttattttgcc cgccccgact ccgtgattga
    39481 cggcgtgtcc gtttaccagt cgcgcttgga tatgggcgtg tccttggcgg aaaaaatcaa
    39541 acgcgagctg cccgtggacg gcatcgacgt cgtgatgccc attcccgaca ccagccgccc
    39601 cagtgcaatg gaacttgccg tccacctcaa aaagccttac cgcgagggtt tgattaaaaa
    39661 ccgctatatc ggccgcacct ttatcatgcc cggtcagtcg acgcgcaaaa aatccgtgcg
    39721 ccaaaaactc agcccgatgg aaaccgaatt tgcaggcaaa agcgtgttgc tggtggacga
    39781 ctccatcgtg cgcgggacga ccagccgcga aatcgtcgaa atggtacgcg cggcgggcgc
    39841 gcgcaaagtc tatatcgcct ccgccgcgcc cgaagtgcgc tatcccaatg tgtacggcat
    39901 cgatatgccc acgcgcgaag agttgattgc caacgggcgc agcgcggcgg aaatcgccgc
    39961 cgaaatcggc gcggacggca tcgtgtttca agacttgggc gatttggaag ccgtcgtcaa
    40021 agcactcaac ccgaaaatcg aatccttcga ttcgtcctgt ttcaacggca tttatcggac
    40081 cggcgacatc gatgatgcct acctcgaccg cctgtctgcc gagaaatccg gctgcggcgg
    40141 tctgaaaatc cacccgagca ggatggaaca cagcatcagc atcagcatca gcgatgcggg
    40201 cgacgaagaa taaggcttcc caaaccgcga tgccgtctga aatccgaacg ttgggttttc
    40261 agacggcatt tcccatatcg gcatcacatt tcccgtttca tccgcacacg atatgagtac
    40321 cgaaaccaaa aactacatta cgcccgccgg ctggcaggca ttgaaagacg agctttatca
    40381 actggtcaat aaagaacgcc ccgaaatcgt ccaaatcgtc aactgggcgg caggcaacgg
    40441 cgaccgcagc gaaaacggcg actatcttta cggcaaacgc cgtatgcgcg aaatcgaccg
    40501 ccgcatccgt ttcctgacca aacgtttgga ggctgccgtc gtcgtcgatc ccgaattgcg
    40561 cgaagcgacc gaccaagtat ttttcggcgc gaccgtcggt ctgttgcgcg gcgacgggcg
    40621 cgagcaaacc gtcaaaatcg tcggcgtcga tgaaatcgat accgcgcaaa acaaaatttc
    40681 ttggatttcg ccgttggcgc gttgcctgat taaagcgcgc gaaggcgacg aagtggtttt
    40741 gaacacgccg gaaggccgtg aggaaattga aatcctttct gtcgaataca tcaaaatcga
    40801 ctgatcggga atgccgtctg aaacggcaca agcgcaccgt ttcccaaata aaggaatacc
    40861 gaatgaggaa aatccgcacc aaaatctgcg gcatcaccac accggaagac gcactgtatg
    40921 ccgcccacgc cggcgcagac gcattgggac tggtttttta cccccaaagc ccccgcgcta
    40981 tcgacatcat taaagcacaa aaaatcgccg ccgcactgcc gccgtttgtc agcgttgtcg
    41041 cccttttcat caacgaaagc gcgcaaaaca tccgccgcat ccttgccgaa gtgccgatac
    41101 acatcatcca attccacggc gacgaagacg atgcattctg ccggcagttc gaccgcccct
    41161 atattaaagc cattcgtgtt cagacggcat cagacatccg aaacgccgcc acgcgcttcc
    41221 ccaacgctca ggcactgctg ttcgatgcct atcacccttc ggaatacggc ggcaccggac
    41281 accgcttcga ctggacgctg ttggcggaat attcgggcaa gccgtgggtg cttgccggcg
    41341 ggctgacccc tgaaaacgtc ggcgaagccg tccgcatcac cggagcggaa gcggtcgacg
    41401 tatccggcgg cgtggaagcg tctaaaggca aaaaagaccc cgccaaagtc gccgccttta
    41461 tcgcaaccgc caaccgccta tcccgttaaa gcaacaaaaa ttgccgccgg aatgacttat
    41521 agtggattaa caaaaaccag tacggcgttg cctcgccttg ccgtactatt tgtactgtct
    41581 gcggctcgcc gccttgtcct gatttttgtt aatccactat aatctcaa
//
LOCUS       DAXFZR010000006        41608 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000006 DAXFZR010000000
VERSION     DAXFZR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41608
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.006"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..971)
                     /locus_tag="ACFJAS_000234"
     CDS             complement(<1..971)
                     /locus_tag="ACFJAS_000234"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3981593.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGEVLVRTKGTKITAGQMKEM
                     HKDNAPLKIFAGGKTFALVDFDDVPDKDIHREPSIIVKSRGIIEFEYYDKPFSHKNEM
                     WSYHSVNKHIYIKYVYYFLKTQENYFRNIGSKMQMPQIATPDTDNYKIPIPSLETQQK
                     IVKILDKFTELEATLEATLEAELALRKRQYRYYRDLLLDFDNQIGGGIADGYQCRLKN
                     VVWKTLGEVAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSGYVPSEGKMTEYIVN
                     DILIGNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQVLA"
     gene            complement(1138..1989)
                     /gene="dinD"
                     /locus_tag="ACFJAS_000235"
     CDS             complement(1138..1989)
                     /gene="dinD"
                     /locus_tag="ACFJAS_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFC3981594.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLRRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLGKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            complement(2105..3649)
                     /locus_tag="ACFJAS_000236"
     CDS             complement(2105..3649)
                     /locus_tag="ACFJAS_000236"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC3981595.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQGIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSIDWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTCIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREVIDIRQLNAEISETVAKIERLRREIDEV
                     IAEIET"
     gene            complement(3724..4728)
                     /gene="rfaD"
                     /locus_tag="ACFJAS_000237"
     CDS             complement(3724..4728)
                     /gene="rfaD"
                     /locus_tag="ACFJAS_000237"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC3981596.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAAAVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            complement(4792..5754)
                     /gene="hldA"
                     /locus_tag="ACFJAS_000238"
     CDS             complement(4792..5754)
                     /gene="hldA"
                     /locus_tag="ACFJAS_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC3981597.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            complement(5810..6550)
                     /gene="pyrF"
                     /locus_tag="ACFJAS_000239"
     CDS             complement(5810..6550)
                     /gene="pyrF"
                     /locus_tag="ACFJAS_000239"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC3981598.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            complement(7074..7721)
                     /gene="adk"
                     /locus_tag="ACFJAS_000240"
     CDS             complement(7074..7721)
                     /gene="adk"
                     /locus_tag="ACFJAS_000240"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC3981599.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            7936..8775
                     /gene="htpX"
                     /locus_tag="ACFJAS_000241"
     CDS             7936..8775
                     /gene="htpX"
                     /locus_tag="ACFJAS_000241"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC3981600.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(9011..10309)
                     /locus_tag="ACFJAS_000242"
     CDS             complement(9011..10309)
                     /locus_tag="ACFJAS_000242"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC3981601.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(10414..11565)
                     /locus_tag="ACFJAS_000243"
     CDS             complement(10414..11565)
                     /locus_tag="ACFJAS_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC3981602.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSAALLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVSVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            11900..13279
                     /locus_tag="ACFJAS_000244"
     CDS             11900..13279
                     /locus_tag="ACFJAS_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     NorM"
                     /protein_id="HFC3981603.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHKAV"
     gene            13323..14363
                     /gene="murB"
                     /locus_tag="ACFJAS_000245"
     CDS             13323..14363
                     /gene="murB"
                     /locus_tag="ACFJAS_000245"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC3981604.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPASFSL"
     gene            14375..15025
                     /locus_tag="ACFJAS_000246"
     CDS             14375..15025
                     /locus_tag="ACFJAS_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC3981605.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            15080..15856
                     /locus_tag="ACFJAS_000247"
     CDS             15080..15856
                     /locus_tag="ACFJAS_000247"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3981606.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDAFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            15906..16472
                     /locus_tag="ACFJAS_000248"
     CDS             15906..16472
                     /locus_tag="ACFJAS_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC3981607.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIQKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            16492..17382
                     /locus_tag="ACFJAS_000249"
     CDS             16492..17382
                     /locus_tag="ACFJAS_000249"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC3981608.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            17407..18165
                     /locus_tag="ACFJAS_000250"
     CDS             17407..18165
                     /locus_tag="ACFJAS_000250"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC3981609.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(18220..18885)
                     /locus_tag="ACFJAS_000251"
     CDS             complement(18220..18885)
                     /locus_tag="ACFJAS_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC3981610.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKLFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAVEKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALANMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAAIFGYRVQEIVTKARRPLEETV
                     IWA"
     gene            complement(19082..19855)
                     /gene="folE2"
                     /locus_tag="ACFJAS_000252"
     CDS             complement(19082..19855)
                     /gene="folE2"
                     /locus_tag="ACFJAS_000252"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC3981611.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            20059..21216
                     /locus_tag="ACFJAS_000253"
     CDS             20059..21216
                     /locus_tag="ACFJAS_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC3981612.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(21280..22281)
                     /gene="hemB"
                     /locus_tag="ACFJAS_000254"
     CDS             complement(21280..22281)
                     /gene="hemB"
                     /locus_tag="ACFJAS_000254"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC3981613.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(22465..22749)
                     /gene="yhbY"
                     /locus_tag="ACFJAS_000255"
     CDS             complement(22465..22749)
                     /gene="yhbY"
                     /locus_tag="ACFJAS_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC3981614.1"
                     /translation="MADTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            22859..23479
                     /locus_tag="ACFJAS_000256"
     CDS             22859..23479
                     /locus_tag="ACFJAS_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC3981615.1"
                     /translation="MAVRSKSSKAWLHEHINDQYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            23543..25510
                     /gene="ftsH"
                     /locus_tag="ACFJAS_000257"
     CDS             23543..25510
                     /gene="ftsH"
                     /locus_tag="ACFJAS_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC3981616.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIQTLLNKNVRVKVTPEEK
                     PSALTALFYSLLPVLLLIGAWFYFMRMQAGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            25732..26163
                     /locus_tag="ACFJAS_000258"
     CDS             25732..26163
                     /locus_tag="ACFJAS_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC3981617.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFINLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            26156..26434
                     /locus_tag="ACFJAS_000259"
     CDS             26156..26434
                     /locus_tag="ACFJAS_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC3981618.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            26487..27065
                     /gene="pth"
                     /locus_tag="ACFJAS_000260"
     CDS             26487..27065
                     /gene="pth"
                     /locus_tag="ACFJAS_000260"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC3981619.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            27126..27332
                     /locus_tag="ACFJAS_000261"
     CDS             27126..27332
                     /locus_tag="ACFJAS_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC3981620.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            27329..27691
                     /locus_tag="ACFJAS_000262"
     CDS             27329..27691
                     /locus_tag="ACFJAS_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC3981621.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            27920..29335
                     /locus_tag="ACFJAS_000263"
     CDS             27920..29335
                     /locus_tag="ACFJAS_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC3981622.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLDLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            29455..29964
                     /locus_tag="ACFJAS_000264"
     CDS             29455..29964
                     /locus_tag="ACFJAS_000264"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3981623.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            30132..31514
                     /locus_tag="ACFJAS_000265"
     CDS             30132..31514
                     /locus_tag="ACFJAS_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC3981624.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIALGRDGRLSGPELMEHIQRGLTDSGISVLNVGMVTTPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(31570..32325)
                     /locus_tag="ACFJAS_000266"
     CDS             complement(31570..32325)
                     /locus_tag="ACFJAS_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3981625.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDG
                     GVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(32335..33051)
                     /locus_tag="ACFJAS_000267"
     CDS             complement(32335..33051)
                     /locus_tag="ACFJAS_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3981626.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(33041..33868)
                     /locus_tag="ACFJAS_000268"
     CDS             complement(33041..33868)
                     /locus_tag="ACFJAS_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3981627.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVARNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            34153..35061
                     /locus_tag="ACFJAS_000269"
     CDS             34153..35061
                     /locus_tag="ACFJAS_000269"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC3981628.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            35138..38740
                     /gene="recB"
                     /locus_tag="ACFJAS_000270"
     CDS             35138..38740
                     /gene="recB"
                     /locus_tag="ACFJAS_000270"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC3981629.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIARALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRQTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            38779..39138
                     /locus_tag="ACFJAS_000271"
     CDS             38779..39138
                     /locus_tag="ACFJAS_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC3981630.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            39263..39742
                     /locus_tag="ACFJAS_000272"
     CDS             39263..39742
                     /locus_tag="ACFJAS_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC3981631.1"
                     /translation="MKHILPLIAASALCISTASAHPADKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(40017..41396)
                     /gene="radA"
                     /locus_tag="ACFJAS_000273"
     CDS             complement(40017..41396)
                     /gene="radA"
                     /locus_tag="ACFJAS_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC3981632.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
BASE COUNT         9628 a        11975 c        10614 g         9391 t
ORIGIN      
        1 tcggccaata cctgatacag atattttgga ttaacctttt catctgttac ccggataacc
       61 aaaacatcac cgtttgttcc gcccgtgcag tctgcctgcc agattttttt caaatacgga
      121 cggatatttc caatcaaaat gtcattgaca atatattctg tcatttttcc ttcgcttgga
      181 acataaccgg ataacttttt accttctcta ttttgtaaga gattatccac gccaacgtaa
      241 ttatgttcgt tcagtttatc cgaacaaata cggttttttg aatattcggc aacctccccc
      301 aacgtcttcc aaaccacatt tttcagacgg cattgatagc cgtcagctat cccccccccg
      361 atttgattgt caaaatctaa aagtaagtcg cggtaatacc ggtattggcg tttgcgcagg
      421 gctaattccg cttccagcgt agcttccagc gtagcttcca gctcggtgaa tttgtcaagt
      481 atttttacaa ttttttgttg ggtttccaat gatgggatgg ggattttata gttatctgta
      541 tctggtgttg ctatttgtgg catttgcatt tttgatccga tattacgaaa atagttttct
      601 tgtgttttta agaaataata aacatatttt atataaatat gtttattaac tgaatgataa
      661 gaccacattt catttttatg cgaaaatggt ttatcgtaat attcgaattc aataatacct
      721 cttgatttaa cgataataga aggctctcta tggatatcct tatcaggaac atcatcaaag
      781 tcaactaatg caaaagtttt tcctcctgca aaaatcttta acggcgcatt atctttatgc
      841 atttctttca tttgtccagc agttatctta gttccttttg tacgaactaa aacctccccc
      901 aatggcttcc actccaccgg tgccgtctga atcatctcaa tcaatttttt cgctttgctt
      961 tgcatatcca tggttgtttt ccgtttcctt cccgctgcgg cgggatatgg ggcggttgcg
     1021 gggcgggttt cagcccgcta tgtccgtttg ccgttgtttc tgcgggctga agcccgtcct
     1081 gctttcaggc tgcctgaaat cgtgtgcagg ctgctttttc aggtagcctg aatgcggtta
     1141 ttttttgccg ttttgatgct gttcggttgc ggcaagcctt ttctgttctt cgttctcaag
     1201 ctgtttgatg cttttctcgg gtacgggctg gttttcaggc atgatgccgc ccagttcttc
     1261 aatggtttgg cgcacttttt gtccgacttc gaaatgcacc cggtttgcct gtgttttgcc
     1321 ttggatgttt ttgcggcgta gtttttcttc ggtttgcgtg gcgcgaaaca gattggcagc
     1381 cggttcgctg gcgttcatgt ggtctaggat tctctggctt ttcttcaagc ctttgcggct
     1441 gtgtatgccc tgcttgccca gtcctccgta tagcccccga tagccgtggt tttggaagac
     1501 ggcgtactca acgggttttt ctacgcctgc atccttggct gcggcggcaa ggtcggtatt
     1561 gtgttcgcgc agctgcctgc gaagcagcag gcgttgtttg tcttcgccca aactcctgaa
     1621 tgctgcctcg tcttgcagct cttggcggcg ggcttgcacg gcaaagtagg tttgccctgc
     1681 ggcaatgacg cttttggacg gatcgccgtt ttgcacaatc aggtagcagg catagcggga
     1741 aaggcggtaa tccgccactt cgcgctgccc gcctttccca agggctatca ttttgttggt
     1801 ttcaacaaaa tgatgatttt tgtcatttcc tgatgtttcg caagcggtta tggctttatc
     1861 aatggcccgt tggaaattgc gccattcgga atattccaag atttgctgca aggtgcgcgc
     1921 cgaccaatat tcattgcctg tttcgtcgat gtgtttggcg ttttcaaaag cgttattttc
     1981 tgttgtcatg tattttcccc gaaaatttct tgatttgaac ttgtaaggat tgcttacagg
     2041 ttgccgcccg tattttcaga cggcctcttt cccattcagt tgttgcaaaa tatgcaacaa
     2101 ctgcctaggt ttcaatctct gcaatcactt cgtcaatttc acgccgcagc cgttcgattt
     2161 tggcgacggt ttcgctgatt tcggcgttga gctgtctgat gtcgatgacc tcgcgggtgt
     2221 cttcggcttc gacatagctg ctgacggcga ggttgtagcc gttgtctttg acggtttgct
     2281 gggcggcgtt ttgggcgata tgcggcacat cggctttgtc ggcgaagagt ttgacgattt
     2341 cggcaatgtg ttcttcggtt aagacgttgt tgttggtttc ttttttaaag aagccgcctg
     2401 catcgatgaa ttggatgtcg gtattgtctt tgtgtttgga caaaaccagg atattgacgg
     2461 cgatgcaggt gccgtaaaag agattgggcg caagggcaat cacggtttcc acatagttgc
     2521 cctccaccag atattggcgg attttctgct ctgcgccgcc gcgatagaaa atgccgggga
     2581 atgagacgat agcggcgcgg cctctgccgg aaaggtagtt cagtgcgtgc aggatgaagg
     2641 caaaatcggc tttggatttc ggtgcgagta cgcctgcggg ggcaaagcgg tcgtcgttga
     2701 tcaaggtggg gtcgtcgctg cctatccagt cgatggaata gggcggattg gagacgacgg
     2761 catcaaaggg tttgctgtct ttgagtttgg ggttggtcag cgtgtcgccc aattcgatgt
     2821 ggaatttgtt gtaattgacg ttgtgcagaa acatattcat gcgggcgagg ttgtaggtgg
     2881 tgtggttgat ttcctgcccg aagaagcctt cttcgatgat gtgttcgtca aactgttttt
     2941 tcgcctgcaa gagcaggctg cccgagccgc aggcggggtc gtagattttg ttgactttct
     3001 cctgcccgtg caccgccagc cgcgcaatca gcttggagac gctttgcggg gtgaaaaatt
     3061 cgccgccgga tttgcctgcg ttggcggcgt agttggaaat caggtattcg taggcatcac
     3121 cgaaaaggtc gatgcggtgg tcttcaaaat tgccgaaatc gagttccgcc acgcctttaa
     3181 ggacggcggc aaggcgtttg tttttgtcgg caacggtgct gccgagccgg ctgctggtgg
     3241 tgtcgaagtc gtcaaacaag cctttgatgc cttgttcgga cgggtagccg gaggcggagc
     3301 tttcaatcgc ggtaaagatt tctttcagct tggtgttgag ctcttcgttt tgatgggctt
     3361 cggcggcaat attgcaaaaa agctggccgg ggtagatgaa atagcctttg actttgacgg
     3421 catcgtcttt gatttcgggc gtgatgatgc tgtccggcat agcggcgtaa tcaatgctgc
     3481 tgtcgccggc ctgcatatag tcggtgaagt tttcgctgat aaagcggtag aaaagtgtgc
     3541 cgagaacgta ttgtttaaag tcccagccat ccaccgcgcc gcgtacttcg tcggcgattt
     3601 tccaaatttg gcggtgcagt tgggcgcgtt gttgcatttc ggtcatcatc gaaatccata
     3661 tataaagtta aacaaatcaa aatcgcctga tattttcaga cgattttttt acgggcattc
     3721 aaattaagcc aaattttcca gcatccattt gacgtagcgg ttgacacctg ctttgacatc
     3781 gaaaaattct tccttatatc cggcttcgcg caatttggtg atgtcggctt gggtgaagcc
     3841 ctggtatttg cctttgagcg cgtcgggaaa ggggatatag cggataagtt cttcttctac
     3901 caactctttc aagctcaatt cagatttgcc ttcggcggcg cggcaggcgt tgacggcggc
     3961 ggcggcgagt tcgttgaact gttggctgcg gccggtgccg aggttgtaga tgccggaaag
     4021 ttcgggatgg tcgaagaagt agaggttgat tttggcgacg tcttcgacgc tgacgaagtc
     4081 gcgggtttgt tcgccgttgc cgtagccgtc gttgctgccg aacaggttga cgtaaccgtg
     4141 ttcgcggtat tggtggaaat ggtggaaggc gacggatgcc atgcggcctt tgtgttgttc
     4201 gtgttgtccg taaacgttga agtagcggaa gccgacgact tgggcggtga gaccttcttt
     4261 catgcggcgg cgcaatactt ggtcaaacag gaatttggag tagccgtaca cattaagcgg
     4321 tttttcgagt tcgcgctctt cgcggaagat ttcgcctttg ccgtaaaccg ccgcactgga
     4381 ggcataaagg aaggggatgc gttcgtcctg acaccagtcc aacaaatcca gcgtgtactg
     4441 gtagttgttt tccatcatat acaaaccgtc gtggttcatc gtatcggaac acgcgccttg
     4501 atggaaaacg gcttcgatgt tttgataagg taaaatgtgt tccctcactt ggcggataaa
     4561 ttcgtgtttg tcgaggtagt gggcgatttc gcactcggca aggtttttga atttttcgcc
     4621 ttttgtcaga ttatcgacgg cgacgatgtc ggtaatgccg cgttggttga gggctttgac
     4681 gatgttgctg ccgataaagc cggccgcgcc tgttacgatg atggtcatat tcggtttcct
     4741 ttgttttgag ggtttcagac ggcattggat ggtcatgccg tctgaaaaga tttattgccc
     4801 gtccagtgct tcaaccaact ctgcaaacga gcaaaccgcc gtaccgagtt tcgccacgac
     4861 aaccccggcc gcagtattgg caaggtacat ggcttcgggc atggtgcagc ctgccgccag
     4921 ccccaagccc attccggcaa tgacggtgtc gcctgcgccg gacacgtcgt aaacttcttg
     4981 ggcgcgggtg ggctggtaaa tgggttcgcc ttcgctgaac agggtcatgc cctcttcgct
     5041 ccgggtcagc aaaacggcgg tcaagtcgag gtggcggcgc aggttttgcg ctttttcggt
     5101 cagatcgcct tcgtttttcc aactgccaac cacttctttc aattcggcgc agttgggcgt
     5161 aatcagagtt gcgccgacat atttttcgta atcgtcgcct ttggggtcga ttaagacggt
     5221 tttgccgaca tgtttcgccc aatcgatcat atcggagata tgcgacagac cgcctttgcc
     5281 gtagtccgaa aaaatgattg cgtcgtattc gggcaagatt tcgcggtatt tccgcttgat
     5341 ttgttccaac acttcgcggt tgggatgttc ttcaaaatca aggcggataa gctgctggtt
     5401 gcgggcgacg acgcgcagtt tgacggtggt ggcgatttgt ttgtcgcgca tcagatagga
     5461 ggcgacgccg tcctgcacca tcagcgcgtc gagcgcggcg gcggcttcgt cgtcgccggt
     5521 tacggacaac agccctgccc tgccgcccaa cgaagcgatg ttgcgcgcaa cgttcgccgc
     5581 gccgcccgcc cgttggtcga ttcgtccgat ttttgccacc ggcacgggtg cttcgggcga
     5641 aattcgggac acgtcgccaa accaatagcg gtcgagcatc acgtcgccga caacaagaac
     5701 tttggcttgt gcgaaacggg atttgagggt ttcttgttgg aacttggcgg gcatattgcc
     5761 tccgttgtgt tttgttcaga caaccttaac ctgtaagacc gcctgaaaat cagtttgctt
     5821 caaggtttgc cacgcggttc acttcgcgca atacggcgac cggatcggcg gctcgggtaa
     5881 cggggcggcc catcaccaga taagtcgaac ccgccgccaa ggcttcggcg ggtgtcataa
     5941 tgcggcgttg gtcgtcgttg ttgccggcaa cgtccaagcg gatacccggt gtaaccaaga
     6001 caaaatcccg tcccaattcg cggcgcagcg gcgcggcttc ttgggcggaa cagaccacgc
     6061 cgtccaagcc cgaactttgt gccagttttg ccaaacggat gacttgttct tcaggggcgg
     6121 tgttcaaacc gatttccgcc aaatcgcttt gctccatgct ggtcaacacg gttacgccga
     6181 ttaagagcgg tttcgtgccg tatccggcaa cggcttcggc ggcggcttcc atcatacgtc
     6241 tgccgcccga cgcgtgcata tcgaccatcc acacgcccat atcggcggcg actttacacg
     6301 cctgagcgac ggtgtgggga atatcgtggt atttcaaatc aagaaagagt ttgaaaccct
     6361 gatgaatcag gctttccgcc aaactgcgtc ccgtcgcggt aaacagctct ttgccgattt
     6421 tgatttggca caacgccgga tcaaggttgc ggacgaatcc gagcgtgtct ttttcgttgg
     6481 caaaatcaag ggcaacgatg acgggggtac gttgttgcgg agtttggaag tcggtgatta
     6541 aaggattcat ggtgttttcg tttcgggttt tcagacgacc tttatcgtct ttttcgcagt
     6601 atagcggatt aacaaaaacc ggtacgtcgt tgccccgccc cggctcaaag ggaacggttc
     6661 cctaaggtgc ccaagcacca ggcgaaccgg ttccgtacta tccgtactgt ctgcggcccg
     6721 ccgccttgtc ctgatttttg ttaatccgct ataacaacgt aaattcaaac cgtaggccgg
     6781 gcattgatgt ccgacctacg cggactcaag cccccgacaa actgtttcgg ataaatttga
     6841 accggtacat cagccgtcat tcccgcgcgg gcgggaatcc attgataaaa ctcagtttat
     6901 cagatttaaa cagcgattgc ccaaattcag aaatggattc ccgcctgcgc gggaatgacg
     6961 ccgaatgatt atttttcggt tgcaacacgc tgtttatcaa aaacaggtcg tctgaaaacc
     7021 tgattttctg ttttcagagt gcgaagcctg cccgtgggta cgaccttttc gatttatttg
     7081 cccaatgcgc ccaatacttc ggctttcaca gcttctactg cttgggtacc gtcgactttg
     7141 atgtatttcg gcgcgtgttc gccttccagc ttgctgtaaa aatcaaccaa aacttcggtt
     7201 tgctcgtggt aaacggcaag acgttttttc acggtttctt ctttgtcgtc gtcgcgctga
     7261 atcaaatctt cgccggttac gtcgtctttg ccttcggttt taggcggatt gtaggtaacg
     7321 tggtaagtac ggccggaagc caaatgcacg cggcggccgc tcatgcggtc gacaatcacg
     7381 ctgtcggata cgtcgatttc aacgaccgcg tccaaaccca cgcctgcttc caccatcgct
     7441 tcggcttgcg ccaatgtacg cgggaaaccg tcgaacagga aaccgttttt gcagtcgtct
     7501 tgcgcgatgc gttctttgac catgccgata atgatgtcgt cgcgcaccaa accgccttca
     7561 tcgatgattt ttttcgcttc caaacccaag ggcgtgcctg ccttaatcgc ggcacggagc
     7621 atgtcgccgg tagagatttg cggaatgccg aacgcggcgg tgatgaattg cgcctgagtg
     7681 cctttgcccg cgcccggtgc gcctaaaagt aatgctttca tgaagtgatc cttttaaatg
     7741 tgtttattgg aatcaaacag atggtttacg attctacacg gcttggcgca atagttcaaa
     7801 aaccaactgc acaaactgcc atttgcaagc aaatgttttg catacctttg ccgagcctga
     7861 tattcacaag gcggcaactt tttgggacaa tccccaaagt ttcaaaacat cacaccctac
     7921 caaaaaggaa catccgtgaa acgcattttt ctgtttttgg ctaccaatat cgctgttttg
     7981 gtcgtaatca acattgtttt ggcggttctg ggcatcaaca gccggggcgg cgcgggcagc
     8041 ctgttggcgt attccgccgt cgtcggcttc actggttcga ttatttcgct gctgatgtcc
     8101 aaatttatcg ccaaacaatc ggtcggtgcg gaagtcatcg acacgccgcg caccgaagaa
     8161 gaagcctggc ttctgaacac tgtcgaagcc caagcgcggc aatggaacct gaaaacgcca
     8221 gaagtcgcca tctaccactc ccccgaaccc aatgcctttg ccacgggcgc atcgagaaac
     8281 agctccctga tcgccgtcag caccggtttg ctcgaccata tgacgcgcga cgaagtggaa
     8341 gccgtgttgg cgcacgaaat ggcgcacgtc ggcaacggcg acatggttac gctgacgctg
     8401 attcaaggcg tggtcaatac ctttgtcgtg ttcctgtcgc gcattattgc caacctgatt
     8461 gcccgaaaca acgacggcag ccagtcccag ggaacttatt tcctagtcag catggtattc
     8521 caaatcctgt tcggcttcct tgccagcctg attgtcatgt ggttcagccg ccaacgcgaa
     8581 taccgcgccg acgcgggcgc ggcaaaactg gtcggcgcac cgaaaatgat ttccgccctg
     8641 caaaggctta aaggcaaccc ggtcgatttg cccgaagaaa tgaacgcaat gggcatcgcc
     8701 ggagatacgc gcgactccct gctcagcacc cacccttcgc tggacaaccg aatcgcccgc
     8761 ctcaaatcgc tttaaaccga tttgaaacga caaaaaaccg tacgcacaag cagtacggtt
     8821 ttttcgtata tgccgactga aaacatcagg tttccatacc ggcaccaacc gtatccattt
     8881 ccgcaccgga tacgccgcat tgatatatcc ccgacaaacc gtgtccggac agaaaatatc
     8941 agataaaaca gcggtaaaaa tgccgtctga aaccaattag ggcttcagac ggcattttta
     9001 ctgcaaaacc ttatgcgaac ggatgatgca gcacaatcgt ttcttcgcgg tcggggccgg
     9061 tggagacgat ggcgaccggc gcgccgcaga cttcttcaat ccgtttcaaa tatgctttgg
     9121 cattggcagg caatgcgtcg tagcttttca cgccgacagt cgattcgcgc cagccgggca
     9181 tggtttcgta aatcggtttg caggtttcca ccgcatccga accgcaaggc aggatgtcgg
     9241 ttttgccgcc gcctggcaat tcgtagccga cgcagatatt gatggtttca acgccgtcca
     9301 ttacatcgag tttggtaatg cacataccgg aaatgccgtt gatttggatg gagcgtttca
     9361 gggcggcggc atcaaaccag ccgcagcggc gcgcgcggcc ggttaccgaa ccgaattcgt
     9421 gtccgcgctc cgccaaacct gcgcctactt cgtcgaacaa ttcggtcggg aacgggcccg
     9481 aaccgacgcg cgtggtatag gctttgacga tgcccaaaac ataatccagc atttgaggac
     9541 ctacgcccgc gcctgccgaa gccgcgccgg cgagacagtt ggacgaggta acgaaggggt
     9601 aagtgccgta gtcgatgtcc aacaacgcac cttgcgcgcc ttcaaacagc agtttttcgc
     9661 cgtttttgtt tttttcgttc aacacgcggg acacgtcggc aatcatcggc gcaatgcgcg
     9721 gcgcgacttt ttcgataacc gccatcacgt cttccgcttt aaccggtccg gcgttatgca
     9781 ggtattggag ttggacgttg taataggcaa ggacggcatc cagtttttca cgcagttttt
     9841 caggatgcag caaatcggcg gcgcgaatgg cgcggcgtgc cactttgtct tcgtaggcag
     9901 gaccgatgcc gcggccggtc gtgccgattt tgcctttgcc gcgcgatgct tcgcgggctt
     9961 ggtcgagcgc gatgtggtaa ggcaggatca gcgggcaggt cggcgcgatt ttcagacggc
    10021 cttcgacgtt tttcacgcct gccgcgttca actcgtcgat ttcgcccaac agggcttcgg
    10081 gggaaacgac aacgcccgaa ccgatgaagc aatccaatcc ttcgtgcagg ataccgctcg
    10141 gaatcaggcg caaaatggtt tttttgccgc cgacgaccaa ggtatggccc gcattgtggc
    10201 cgccttggaa gcgcacgacg ccgccggctt cttccgccag ccagtcaacg attttacctt
    10261 taccctcgtc gccccactgt gcgccgatta ctacaacatt tttagccata gccatataac
    10321 ctatcgatat taaaaattat tgcggaaaat ccccaaaccg gtgcggcttt gcgccccgcc
    10381 ccggtttgcc tttcatccgc tatgaacggg tatttagcgt ttcacgacct gccaaacgcc
    10441 gtccgtcttt ttcagacggc ctgcaagctc ttccgaaaca ttgtgtccga taccgtagtc
    10501 aatcacgaca cactgccctt gttcacgcaa ggcttcgacc gcttcgcgcg ccgcttctgc
    10561 atcttccgca tcgaccgaca cggcgggctg ccgttcgacg gcgggcaaac gcccgataaa
    10621 gctgcgtaag tcgaaactga atcccgttgc cgggcgcgcc ctgccgaaat atccgcccaa
    10681 tccgtcataa cgcccgccgc gcgcgaccgc gtcgtggaaa tcggcggcat aggcggcata
    10741 cagcaagccc gtgtggtaat tgtcgacgcg cagctcggac aagtcgatat ggatttcatt
    10801 atcggggaat gcgtcgcaca ccgcctgcaa ttcgtccaac gcgccgccga ccgccgacaa
    10861 atccggcaac cgtccgcgcg cgtcggacaa cacttcacgc ccgccgtaca ggcgcggcag
    10921 cagcgagaat gcttttgccc acataccgtc cagcttccaa gccttgacct gcgcttcgac
    10981 cgacccggta tctttatcct gcatcaaggc aagcagcgct gcggactgcc ccgcatccaa
    11041 atgcgccgca tcggacaagg cgcggaatat gccgatatgc cccagcgaaa gcagcacttt
    11101 gcccatatcg gcaattttca tgcttttcaa cataaggtct atcagctcga tgtcgccacg
    11161 gatgtcggca aaaccgtaca tttccgcccc tgcctgcaag ggttcgcgca tattcggcag
    11221 accgtcgggc tgcgcgtgca acaccggacc ggcataacac aaccggttaa tcccttggtt
    11281 ggcggacaaa agatgggcat cgatacgcgc cacctgcggc gtgatgtcgg cgcgtatgcc
    11341 caactgcctg ccgctgagcc tgtccgttac caaaatggtt ttcagtgaaa gccccgcatc
    11401 gatatgcgtc agcagggaat gtgcgtactc catcagcgga ggctgtacca gttcataacc
    11461 gtgtacgcgg aacagtgcca acaactgctc gcgcgcgctt tcaagctgcc gcgcgttcgt
    11521 gggcagtacg tcggcgatat gttccggaag ctgccatgtc tgcatgagaa tctgccttct
    11581 gttttatcct gtaaacacaa aagactgccc gatccgcaat cagacggcat ctttgccgca
    11641 acgcatcacg ccgctgtcaa aacggcgatg cttccttccg attcagaatc cgaacagccc
    11701 ctgtccgacg gctcaatgcc gtcaagtcgt taaaaccaaa ctttaccata aaatacacac
    11761 aatctgaata tacttttata ccccgtatcc gccgtctgac ggcacggtat tttttactat
    11821 ataatgtgca ccattatttc tcatcacttc ccgctgccgt ttttttgttt cagacggcat
    11881 ttttattgac tgaacactta tgctgctcga cctcgaccgc ttttcctttt ccgtcttcct
    11941 gaaagaaatc cgcctgctga ccgcccttgc cctgcccatg ctgttggcgc aggtcgcgca
    12001 ggtgggcatc ggtttcgtcg ataccgtgat ggcgggcggt gcgggcaagg aagatttggc
    12061 ggcggtggct ttgggcagca gcgcgtttgc cacggtttat attaccttta tgggcattat
    12121 ggcggcgctg aacccgatga ttgcccagct ttacggcgcg ggtaaaaccg gtgaagcagg
    12181 cgaaacgggg cggcagggga tttggttcgg gctgattttg gggattttcg gcatgatttt
    12241 gatgtgggcg gcgattacgc cgttccgcaa ctggctgact ttgagcgatt atgtggaagg
    12301 cacaatggcg cagtatatgc tgttcaccag cttggcgatg ccggcggcaa tggtacaccg
    12361 cgcactgcac gcctacgctt ccagcctgaa ccgcccgcgc ctgattatgt tggtcagctt
    12421 tgcggcgttt gtgttgaacg tgccgctgaa ctatattttc gtttacggca aattcggtat
    12481 gcccgctttg ggtggcgcag gttgcggcgt ggcgacaatg gcggtgtttt ggttcagcgc
    12541 gctggcattg tggatttata tcgccaagga aaaattcttc cgcccgttcg gactgacagc
    12601 gaaattcggc aaaccggatt gggcggtgtt caaacagatt tggaaaatcg gcgcgcccat
    12661 cgggctgtct tattttttgg aagccagcgc gttttcgttt atcgtgtttt tgattgcgcc
    12721 tttcggcgag gattatgtgg cggcgcagca ggtcggcatc agtttgtcgg ggattctcta
    12781 tatgattccg caaagcgtcg gctcggcagg gacggtgcgc atcggctttt cgcttgggcg
    12841 gcgcgaattt tcgcgggcgc gttatatttc aggagtgtcg ctggtgtcgg gctgggtgct
    12901 cgccgtgatt accgtgcttt ccttggtatt attccgttcg ccgctggcaa gcatgtacaa
    12961 cgatgatccg gcagttttaa gcatcgcctc caccgtcctg ctgttcgccg gcctgttcca
    13021 accggcagac ttcacccaat gtatcgcgtc ctatgccctg cgcggttaca aagtcaccaa
    13081 ggtgccgatg ttcatccacg ccgccgcctt ctggggctgc ggcctactgc cgggctatct
    13141 gctcgcctac cgtttcgata tgggcattta cggcttctgg acggcattga ttgcctcgct
    13201 caccatcgca gccgtcgcct tggtgtggtg cttggaaaaa tacagtatgg agttggtcaa
    13261 atcacacaag gccgtctgaa aacgcagcac acttcaattc aaatacagtt aaagctcaaa
    13321 ccatgcaacc catccgatac cgaaccgacc tcacccccta caacactttc ggcctgcacg
    13381 cccaagcccg ggcctttatc gcgcttaaac acgccgacga attgcgcgac atcgtccgac
    13441 tgcccgagtt cgaccgcgat actgttttat ggctgggcgg cggcagcaac atccttttga
    13501 tgcaggatta cgacggcttg gtcgtccata tggaaaacaa gggcatacgc gagattgcgc
    13561 gctcagacgg catggttctg attgaagcgc aggcgggcga aatttggcac gatttcgtgt
    13621 tgcacaccgt cgcgctgggt ttgagcggtt tggaaaacct gagcctgatt ccgggtacgg
    13681 tcggcgcgtc gcccgtgcag aacatcggcg catacggcgt ggaggcgaaa gatgtgattc
    13741 acagcgtgcg ctgcttcgat ttggatacgg aaacctttgt aaccctttcc aatgccgact
    13801 gccgcttcgc ctaccgcgaa agcctgttca agcaggaagg caaagggcgt tatgtgattg
    13861 tttcggtcgt atttgcatta aaaacgcatt ttgtgccgaa cttgggctac ggcgatttgg
    13921 cggcgaaggt tgccgaattg agtgcaggta gggaggcgac ggcgaaagac gtttccgatg
    13981 cggtatccgc catccgcaac agcaaattac ccgacccgaa agttttgggc aatgtcggca
    14041 gtttctttaa aaaccccgtc gtcagcgcgg aaaaagccgc caccctgttg cagcggcatc
    14101 ccgatatgcc gcgctatccg cagcccgacg gttcggtcaa actcgccgcc ggctggctga
    14161 tcgaccaatg ccgtctgaag ggcttccaaa tcggcggtgc ggcggtacat gaccggcagg
    14221 ctctggtttt ggcgaacaaa aacaatgcct cttcagacga catctggaaa ctggcgcaac
    14281 acgtctgcaa tacagtattt actcgatttc aggtagaatt acacgccgaa cccaattggc
    14341 tgcccgcttc gttcagcctg taaatccaag gagtatgccc gtgacccgca ttgcaaaaac
    14401 caatacttac acccgcatca tcgacgccag ccttgcgctt ttcaacgagg aaggcgagcg
    14461 caacatcagc accaaccata ttgccgccca cttgggcatc agtccgggca atctctatta
    14521 ccacttccgc aacaaagacg aaatcatcgt ccaactgttc aaacgttaca gcgaagccct
    14581 gctggaatac ctcaacgaag ccgtgttgcc ctctgatgtg gaagactcta tcaactatat
    14641 ggcaggcatt tacgatgtca tgtgggaata ccgcttcctc tttagcgacg taaacaccct
    14701 gcttgcgcgc agtgccgaat tgttgggcga acacaatacc tttacccaag ccaaagtctc
    14761 gccgcttcta gtcaaactgc tgacccagct caacgggctg aacgtcatca acgccgacca
    14821 aaccgctatg aacgacctcg ccgtcaatat gtggatggtt acgaaatact ggttcgactt
    14881 cgacagttcc ctgcgcggcc gtaccaagct gaccgaagac tccaaagcac gcggcgtccg
    14941 tcgtacctta agcctccttc gcccctatct tttgcccgaa caccgcgcgg aatacgaccg
    15001 caaaatcagc aacggcaacc cgtaaaccca taacgtccga atcagccttc agacggcact
    15061 tacccgacgg tatcagcgaa tgcgcgcagt ccccccgccc catatctcca tcacaggcct
    15121 aggttacctc ggcctgccgc tggcacaaaa gttttaccga cacggcagcc gcgtcgccgc
    15181 cataaaacgc agcctgactt cggacgacat caacctgccc atacacctcg acaccttcga
    15241 cctcaaccgt accgatgcct ttcagacggc attgtggcgg catcacgccg acaaacccgt
    15301 ctggttcttc ctgctgccgc cctccttttt ggcgcattac gccgacaccg tcaaacaatg
    15361 ggcagagctt gcccgggcgt gcaacgtgca acacctgatt ttcacaagca gcacaagcgt
    15421 ttacggcgat aaagcgcgag aatgcaacga aaccgccgca cccgatccgc aaaccgagtc
    15481 cgcccgacaa atccttgccg ccgaacaaca cctgctcgac aacggcgttc cgaacatcga
    15541 catcctgcgg ctgggcgggc tttattgcgc cgaacgccat cccgtcagcc gcctcgtgca
    15601 aaagcaaaac atccaaggcg gcaaccggcc tgtcaatatc gtccaccgcg acatcgccgt
    15661 cgaaaacctg tttcagacgg catccaaccc cggcggcagg cggctgaaaa acattatcga
    15721 accgcgccac ccgacacgcc gcgaatttta tacggaagaa gccgccaaac tcggcctgcc
    15781 cgcgccggat ttctcacccg acgacagcgt gggcaaaatc atccgtaccg tttgcgataa
    15841 cggcttaagc ctgtaaaata agcggcaaca gcaacaacag gcattctccg ccccaatcag
    15901 aaaatatgtc cgccctcctc cccatcatca accgcctgat tctgcaaagc ccggacagcc
    15961 gctcggaact tacctccttt gcaggcaaaa cactgaccct gaacattgcc gggctgaaac
    16021 tggcgggacg catcacagaa gacggtttgc tctcggcggg aaacggcttt gcagacaccg
    16081 aaattacctt ccgcaacagc gcgatacaga aaatcctcca aggcggcgaa cccggggctg
    16141 gcgacatcag gctcgaaggc gacctcatcc tcggcatcgc ggtactgtcc ctgctcggca
    16201 gcctgcgttc ccgcgcatcg gacgaattgg cacggatttt cggcacgcag gcaggcatcg
    16261 gcagccgtgc caccgacatc ggacacggca tcaaacaaat cggcaggaac atcgccgaac
    16321 aaatcggcgg attttcccgc gaacccgagt ccgcaaacac cggcaacgaa gcccttgccg
    16381 actgcctcga cgaaataagc agactgcgcg acggcgtgga acgcctcaac gaacgcctcg
    16441 acaggctcga acgcgacatt tggatagact aaccttcaga cggcatccga catgaacagc
    16501 ccttttcaca acatcggtat cgtaacccgc cccaacacgc ccgacatcca agataccgca
    16561 cacacgctga ttaccttttt gaagcagcac ggctttaccg tctatctcga cgaagtcggc
    16621 gtaagggaat gctgcatcta tacccaagac accgacggct gccatatcgt caacaagacc
    16681 gaactggggc aatactgcga cctggtcgcc gtcttaggcg gagacggcac ctttctctct
    16741 gccgcccgcg aaattacccc ccgcgccgtt ccgattatcg gcatcaacca aggtcatttg
    16801 ggctttctta cccaaattcc ccgcgaatat atgacggaca agctattgcc cgttttagaa
    16861 ggaaaatacc ttgccgaaga gcgcatcctg attgaagccg cactgatccg cgaaggcaaa
    16921 accgccgaac gcgccctcgc cctcaacgat gccgtcctct cccgcggcgg tgccggacag
    16981 atgattgaat ttgaagtctt cgtcaatcag gaattcgtct atacacaacg ttcggacggg
    17041 ctgattgtct ccacccccac cggatcgacc gcctattcgc ttgccgccgg cggcccaatc
    17101 atgcaggcag gattacacgc cttcacgctc gtccccatct gcccgcaatc tatgactaac
    17161 cgccccatcg ccattccaga cacgtccgaa atcgaaatcc tcgttaccca aggcggcgac
    17221 gcgcgcgttc atttcgacgg acaatccttc atcgacgtgc aaaacctcga ccgcatcatc
    17281 atccgccgct accacaatcc cttacgcatc ctacacccga ccgactacca atatttcaaa
    17341 accctgcgcc aaaaactgca ctggggcgag caattagtct aagccggcct tcacccgaaa
    17401 gtacccatga attccaaaat ttcctccgac catacagaag ataccatacg cctctccaaa
    17461 cgtatggcac aactggggct ttgttcgcgc cgcgaagccg acggccatat cgaacagggc
    17521 tgggtaacgg tcaacggcaa aaccgccgta ctcggtcaga aagtttcacc ggcagaccgt
    17581 atcgaactga acaagaaagc ccacgaacag caggcggcac gcattaccat cctgctgaac
    17641 aaacccgtcg gctatgtcag cgcacaagcg gaaaaaggct ataaatccgc cgccgaactg
    17701 attacccctg aaaattgctg ggaaggcgac accagccgca tcagtttcga tccgaaacac
    17761 aaaatcggtc ttgcccccgc cggcaggctc gatatcgatt cagtcggatt gctggtgttg
    17821 acgcaagacg gccgtatcgc caagcagctt atcggcgaaa acagcggcag tgaaaaagaa
    17881 tatttggtgc gcgtgcgcgg caaattggac gaaaaaggac ttgccttact gaatcacgga
    17941 ttgagtttgg acggtgataa actgcgtccc gccaaagtag aatggcaaaa tgaagaccaa
    18001 ctgcgcttcg tgttgaaaca gggtaaaaag cggcaaatcc gccgtatgtg cgaactggtc
    18061 ggactgcgcg tcgtcgggct gaaacgcatc cgcatgggca aggtcaaact cggcaggctg
    18121 ccgcccggca aatggcgtta tctcgctccc ggcgaatcgt tttaaataag cattaaaaaa
    18181 tgccgtctga aaatccttgc gttttcagac ggcactgcct tatgcccaaa taacggtttc
    18241 ttccaagggc ctgcgcgctt tcgtgacgat ttcctgaacc cggtagccga atatcgcggc
    18301 aacggacacg ccccattctg ccgcatcgaa caaaccgaac tgcccggaca atacgcgttc
    18361 catatcggca tagttgaaac cttccaccgg gcaggaatcg atacccgcca tcgccgcacc
    18421 cgtcatcatg ttggctaacg cgatataggt ctggcggcag caccagtcaa acaaggcgcg
    18481 cgaatcgtcc aaaatcttga tgtcgtcagc ttgaaacgcc tgatacctcg ccaaagattt
    18541 ttctacggca tccggttcgg taacgccgcg ccgtttgaga ctttccaaca taaacgggct
    18601 gtcgaagcgg gcatttttct tcgccaaaaa caccaccaaa tgactggcgg tatccaaagc
    18661 atccgccata ccccaagaaa acagcttgat tgcctgtcgg atttcggggt tttgaaccac
    18721 aacaaactgc caaggctccg aaccgaccga actgggcgac aaacgcccga gttctaaaat
    18781 aaactgaaaa tcctcggcac tgattttacg tgccgcatcg taatgccggc acgatttgcg
    18841 gtttttaaat gcggatagaa cctgctcttt gcttaatact gtcatcgtgc atccttttat
    18901 tactttgatt gcaaattgat ttcaaaccga tacggcactg ccgcacccta taagccgtcc
    18961 tcgccatcaa gcttcacccg gattttaccc atctctctac ggctgggaaa acaaagcggc
    19021 ggcagattcg ggaataactc cccaatgcct ttcagacggc atttggacat cgcaaacgcg
    19081 cctacgggta ggcgatatag gcataagccg aatggttgtg gatagactcg aaattctcgc
    19141 tctcgacgac gaaactcttg atgcgtttgt cggcaatcag cgaagtagcg acatcgcgca
    19201 ccatatcttc cacgaatttc gggttttcgt aggccttttc ggtaacgtat ttttcatcgg
    19261 ggcgtttgag caggccgtag agttggcagc tcgcctgcgt ttccacataa tcgataactt
    19321 cctcgatacc gacttcggca tcggaagtca ggctgacggt aacgtgcgaa cgctgattat
    19381 gcgcgccata ttgggaaatt tctttggaac acgggcaaag cgaggttacg ggaatcatga
    19441 ccttcatact gtggccgtat gccccgtctt tcatttcacc cgtgaggctg acatcataat
    19501 ccagtaagga ccggataccg gataccggcg cggttttctt gcggaaaaac ggaaaagaaa
    19561 cgctgatttt gccggcgcgg gaatccaaaa gcgcgaccat ttcggcagtc agcctatgca
    19621 attgtgcgaa atccaagact tcggtatgtt gctccatcaa tgcgacaaag cgcgacatat
    19681 gcgtcccttt ctgctcggcg ggcagataaa ccgtcatcgt caggcgagca accgtggatt
    19741 gtgttccttc ggcggttttc agggtaatcg ggaagcgcag gtctttaatg ccgacctgat
    19801 tgatcggcag gttgcgcaaa tctctgctgg attgcacgtc tgcaatggcg ttcatgcgtt
    19861 gtttgtcctt aatattcaga taattattga gatgtgtgta ttgtatggca ggcagatgcc
    19921 gtctgaaaaa acgctgtcgg ccgtcctgcc tgcaaatgcg agattatatc acttgctttt
    19981 ggcggctgca ttgattgttt ctattttcgg gatagtgata aaatcccgac ttatccatat
    20041 cttacaaggc agaaacagat gaaattcccg acccgtgcca tccattccgg ctacgattgc
    20101 gacgaacaca accgcgcgct gatgccgccg atttatcaaa acagtatgtt tgcgctgcac
    20161 gaaatcggcg aaaatgtgcc ttaccgttat tcgcgcctga gcaacccgac ccgtcagatt
    20221 ttggaagaca ccgttgccga tttggaacac ggtgcggcag gttttgcgtt ttccagcggt
    20281 atggcgggga ttgatgccgt gtggcgtacc ttcctgcgcc cgggcgatac catcgtcgcg
    20341 gtcgccgata tttacggcgg cgcatatgac ttattggtcg atgtttatca aaaatggggg
    20401 gtgaacgttg tttttgccga tttgggcaat cccgataatt tggacgaatt gctcaaagcg
    20461 cacaatgtca aactggtttg gctggaaatg ccgtccaatc cgcttttacg cttggtagac
    20521 atcaaagccc ttgcagcgaa agccaaagca gccgacgcgc tggtcggcat cgacaacacc
    20581 tttgccacgc cgtatctgca acagcccttg gatatgggtt gcgattttgc attccattcc
    20641 gctaccaaat atttgtgcgg ccattccgac gtattgatgg gcatcgtcgt tgccaaaacc
    20701 aaagagctgg ctcagccttt gcacgatatg atggtgcata ccggcgcgat tgcaggcccg
    20761 acggactgct ggctggtgtt gcgcggcatc aaaacgcttg ccctgcgtat ggaggcacac
    20821 tgcaaaaacg cactcgaaat tgcgcgccgt ttggaagccc atcctgccat tgaaaaagtg
    20881 ttccatcccg gcctgccgtc tcacgaacat tacgcactcg cccaagcgca aatgcccaaa
    20941 ggcatcggcg gcgtggttac ggtttatctc aaaaacgaca cgcgcgaagc ggcaaacagc
    21001 gtgattaaaa acatgaaact ggtcaaaatg acctccagcc tcggcggcgt ggaaagtctg
    21061 gtcaaccatt gctattccca atctcacagc ggcgtgccgc ataatgtgaa aatggaaatg
    21121 ggcatcaggg tcggactcct gcgcttctcc atcggtattg aagacgtaga tgatatttgg
    21181 aacgatattt ccgccgcact cgatacaact ttgtaaaccg taaaaaatgc cgtctgaaac
    21241 catagtttca gacggcattt caatattgcg aatcaaatat caacgcttca gcatctttgc
    21301 cgcctcaatg gcgtaatagg tcaaaatccc gtccgcaccc gcacgtttga atgccagcag
    21361 gctttccaaa accactttgc cgccgtccag ccagccgttg gcaaccgccg cctgcaacat
    21421 cgcatattcg cccgaaacct gataggcata agtcggtacg ccgaactcgt ccttcacgcg
    21481 gcggacaacg tccaaatacg gcaaaccggg cttcaccatc accatatccg caccttcctg
    21541 aatatcgagc gccacttcat gcagcgcctc atcggtattt gcaggatcca tctgataggt
    21601 ctttttatct gcctttccca aattgcccga actgcctacc gcatcacgga aagggccgta
    21661 gaatgcagaa gcatatttgg cggaatatgc cataatccgc gtatggatat gtccggcatc
    21721 ctccaaagcc tcgcggatgg cgccgatacg cccgtccatc atatcggaag gagcaacgac
    21781 ctgcgtgccc gcctctgcat gacataaagc ctgtttcacc aagacttcta cggtttcatc
    21841 attcatcacg taaccgtttt cgtccgtcag tccgtcctga ccgtgcaccg tataaggatc
    21901 gagcgcgaca tccgtcataa tgcccagttc gggaaacctc tcgcgcaagg ctcggacagt
    21961 tgacggcacg agtccttcgg gattgtacgc ctcctgcgca cgcccggttt tgtttgccgt
    22021 aaccacggga aagagtgcca acatcggaat accgagcttc accgcctctt ccgccgtaaa
    22081 cagcagcctg tccaaactct gacgcttcac gcccggcata gaaggcacat cctcctcgcg
    22141 cgccgccccc tccaatacga acaccggata aatcaaatca tcggcggtca gcatatgctc
    22201 gcgcatcagg cggcgtgaaa aatcatccct gcgcatacgg cgcatacgcg aagccggaac
    22261 attgcggtaa ggaaattgca taagccctcc aatcatcctg tcaacaattc aaatcatacg
    22321 gaagccgccg caacttgcag acggcatcga gcggtaatca aaatccgttt tcaccggaaa
    22381 gggtttcggc acgccgaaat gccgcgccga aggacagcgt gccgcccatt gaaagaacaa
    22441 tagcaaaaca accacttcag gctgtcaggc ctcgatattc ttacgccaca ataccaaaag
    22501 tttcccgata tgccgaacag gttgcgcgtc aaccgcctca cacaaggtat tgcagatttc
    22561 gatacgctcg gcacggtcgt cgccgaatac gcgcacttta atcagctcat gcgccgtcag
    22621 tgccgcatcg gtttccttga tgactgcgtc cgtcagaccc tgctgaccga ccatcacaac
    22681 aggatggaga tggtgcgcgc gcgctttcag ttccaaaatt tctttggtgt tcaatttggt
    22741 atccgccatt ttcctactgt ttgaataaaa aggataatgc agcattgtac gcgatttggc
    22801 acggcaacgg caaaattaac gtacaataca ccgtttacat tccgacccgc gaaccactat
    22861 ggctgtacgt tccaaatcct caaaagcgtg gctgcacgaa cacatcaacg accagtacgt
    22921 ccatatggcg caaaaagacg gctaccgcgc ccgtgccgca tacaaacttt tggaaatcaa
    22981 cgaaaaagac aagataatca aacccggcac ggtacttgcc gacttgggca gcgcgccggg
    23041 aagctggtcg caggttgccg ccaagctgac gggtacttcc ggagcggttt tcgccttgga
    23101 catcctgcct atggaagcca tagggggcgt gtccttcatt cagggcgact tccgcgagaa
    23161 cgacgttttg gcgcaattcg aaaccttgtt ggacaaccgc ccgctcgacc ttgtaatctg
    23221 cgatatggcg cccaatatgt cgggaaacgc cgtaagcgat caggcacgca gcttttatct
    23281 gtgcgaactg gctttggact tcgcctcgca acacctgaaa accggcggca gctttttggt
    23341 caaagtcttt cagggtgcag gctatcagga atacatggca gccatgcgcg aaattttcgg
    23401 cactgtgcag acgcgcaaac ccgaagcctc gcgcaatcgc tccagtgaga tttatttatt
    23461 gggcaaaaat aaacgctgac aatacagacg gcgtgcttta caatcatttc cgttttacac
    23521 ctcaattatg gagccttgct aagtggggaa cacctttaaa tcaatccttg tctgggtcgc
    23581 cttgggtatc ggcctgatgg ctgcgttcaa cgctttggac ggcaaaaaag aagacaacgg
    23641 gcaaatcgaa tattctcagt tcatccgaca ggtcaacaac ggcgaagtat ccggcgtcaa
    23701 catcgaagga tccgtcgtca gcggttacct gattaaaggc gagcgcaccg acaaaagcac
    23761 cttcttcacc aacgcgccct tggatgacaa cctgattcaa acccttttga acaaaaacgt
    23821 ccgcgtaaaa gtaacgccgg aagaaaaacc gagcgcgctg actgccctgt tttacagcct
    23881 gctgcccgtc ctgctgctga ttggcgcatg gttctacttt atgcgtatgc aggcgggcgg
    23941 cggcggaaaa ggcggcgcat tctccttcgg caaaagccgc gcccgcctgc tggacaaaga
    24001 tgccaacaaa gttacctttg ccgatgtcgc cggctgcgac gaagccaaag aagaagtgca
    24061 ggaaatcgtc gattacctca aagcaccgaa ccgctatcaa agcctcggcg gccgtgttcc
    24121 gcgcggcatc ctgctggcgg gcagcccggg aaccggtaaa acactcttgg cgaaagccat
    24181 tgcaggcgag gccggcgtgc cgttcttcag catttccggt tccgattttg tcgaaatgtt
    24241 cgtcggtgtc ggtgcaagcc gcgtccgcga tatgttcgag caggcaaaga aaaacgcccc
    24301 atgcattatc tttatcgacg agattgacgc ggtaggccgc caacgcggcg caggtttggg
    24361 cggcggcaat gatgagcgcg agcaaacatt aaaccaatta ttggttgaaa tggacggttt
    24421 tgagagcaat cagactgtaa ttgtgattgc ggcaaccaac cgccccgacg tactcgatcc
    24481 tgcgctgcaa cgccccggcc gcttcgaccg ccaagtcgtc gtccccctgc cggacatccg
    24541 ggggcgcgaa cagattttga acgtccattc taaaaaagtg cctttggacg aatctgtgga
    24601 tttattgtcc ctcgcgcgcg gcacgcccgg tttttccggc gcggatttgg cgaacctggt
    24661 caacgaagcc gccctgtttg ccggccgccg caacaaagtc aaagtcgatc aaagcgattt
    24721 tgaagacgcc aaagacaaaa tctatatggg tccggaacgc cgcagtatgg tgatgcacga
    24781 agacgaaaaa cgtgcgacgg cgtatcatga gtccggacac gcgattgttg ccgaaagcct
    24841 gccctttacc gaccccgtcc acaaagtaac cattatgccg cgcggacgtg cgctgggtct
    24901 gacttggcag cttccggagc gcgaccgcat cagtatgtat aaagatcaga tgttgagcca
    24961 gctctccatc ctgttcggcg gacggattgc cgaagacatc ttcgtcggac gcatctccac
    25021 cggtgcatca aacgactttg aacgcgcaac ccaaatggca cgcgaaatgg taacgcgcta
    25081 cggcatgagc gacaaaatgg gcgtgatggt ttatgcggaa aacgaaggcg aagtcttctt
    25141 ggggcgcagc gtaacccgtt cgcaaaacat ttccgagaaa acccagcagg acatcgacgc
    25201 ggaaatccgc cggattttgg acgagcagta tcaggttgcc tacaaaatcc tcgatgaaaa
    25261 ccgcgacaag atggaaacga tgtgcaaagc cctgatggaa tgggaaacca tagaccgcga
    25321 tcaggtactg gaaattatgg cgggcaaaca acccagcccg cccaaggatt acagccacaa
    25381 cctgcgcgag aatgcggacg cggcggaaga taacgcgccg cacgctccga ctcgggaaaa
    25441 aaccgaagca cctgccccgg cagacaccgc ctcgacagag tccgggcagc agcctgaaaa
    25501 caaggcttaa ctccccgaac aaacggcagc ccgcaagctg ccgttttccc atcccgtttt
    25561 cagacggcat tttccgctca atgccgtctg aaacgccaaa gccgtatcgg tttttgcgaa
    25621 tctgcgatat ggcggtttaa attgaaatca gaacggggca aggctgtacc ggtttaaaat
    25681 taaaccgcta taataccgcc cttcgaatac acgcccgcat tggtgatgcc cgtgaaaaaa
    25741 gttgaaaaaa acatcttggt gctgcacggc gcggacaaaa tgttcgagct ggtcgacaag
    25801 gttgaggatt acccgcactt tctgccgtgg tacagcaaga ccgaggtcat cgggcgtagc
    25861 ggcaacgaac tgaaggcgcg gctgtttatg gattatatgc gcgttcgcca atcgtttgcc
    25921 acgcacaacc gcaacatccc cggcagggaa atccgtatgg aactgctcga aggcccgttc
    25981 aaaaccttac gtggaacgtg gaaatttatc aatttgggcg acgatatgtg caaaatcgaa
    26041 ttcaatttgg aatacgattt ttccaatgcc gttttgtccg ccttaatttc ccccgtcttc
    26101 aaccaccttt ccgccacgct ggtcgaagcg ttcgtcaaag aggcagaccg ccgttatgct
    26161 tgaaattgag attgtgtacg gtctgcccga ccgacaggtt ttgaaaacca tgcagcttgc
    26221 cgagggaaca accgtccgca ccgccgcact gcaaagcggt ttggacggca tatttgaaaa
    26281 tttaaacctg cattccgcgc ctttgggcat tttcggcaaa gccgtcaaag acgacacgcc
    26341 gctgcgcgac ggcgaccgca tcgaagtgta ccgcccgctg ttgatcgacc ccaaagaagc
    26401 gcgccgcaaa cgcgttcaaa atcaagaaga ataaccatgc cgtctgaagc cttcagacgg
    26461 cattcgacag aaacacggaa aatatcatgt caaacacaat caaaatggtt gtcggcttgg
    26521 gcaacccggg caaagaatac gaacagaccc gccacaatgc gggcttttgg ttcctcgacg
    26581 aactggcgtg gaaatggaag gcttcgttta aagaagaaaa aaaattcttc ggcgaagttg
    26641 cccgcgccgc cctgcccgac ggcgatgttt ggctgctcaa accggccacg ttcatgaacc
    26701 gttccggaca ggcggttgcc gcgcttgcac agttctacaa aatcaaaccc gaagaaatcc
    26761 tcgtcgtcca cgacgaactc gacatccctt gcggacggat caaattcaaa ctcggcggcg
    26821 gcaacggcgg acacaacggc ttgaaagaca ttcaggcaaa actcggcacg gcagactatt
    26881 accgcctgcg cctcggcatc ggccaccccg gcgaccgcaa cctcgtcgtc ggctacgtct
    26941 tgaacaaacc gagcgcggaa caccgccggc aaatcgacga tgccgtcgcc aaatccctgc
    27001 aggccgtacc cgacatcatt tccggcaaat gggaagaggc aacgcgcttc ctgcacagca
    27061 aatgacccga tgccgtctga agccctttca gacggcattt tcccgatttc cgtatccgaa
    27121 cagtcatgac cgaactcaag cagcttatcc aaaccgaatc catccccgtc atcgaagaaa
    27181 ccctcgattt cctgctgtac gaatgcagca tcgacgacgc accgtccgcc gaagaagtgg
    27241 cacaatggcg cgacatactt gccgcacgcg gcggcaaatt cctgcgcctg tccaaaatct
    27301 gccaaacgtg gctggacgag gaggcggcat gaagctgccg cgcaaccgct tcagcctgct
    27361 ttccgcattg tggtttgccg gcggcatcta ttcgctgctc ttcaaagctg ccgacaccgc
    27421 gccgccgccg tttccacatt tcgacaaagc agcacacctt gccctgtttt tcgcacaaat
    27481 cttgcttctg gccaaagcat tcaaaaccgg aaaacttccc atcccctacc gcagcctgat
    27541 tgcgttcgcc ttctgttttg ccgtcggcag cgaatgcgcg caggcatggt ttactgcaac
    27601 gcgaaccggc agtttgggcg atgtccttgc cgacctgacg ggcgcagccc ttgccctctt
    27661 tgccgcgcgt tctgcctgcc gcccggacta aatcggttta tttctcccaa acaggatgaa
    27721 tccccctgat gcatcctgtt tttcccctta catatcaatc ccatctcata aacaaaaccg
    27781 acaaatcgtt tacaatatat ttacactaca ccgaattaca aatatactcg aagcggttgc
    27841 agaaccgacg gtttcgcaaa ccgtttcgga ttgcacgaca caaaagaaaa accgattttg
    27901 ttgccttaaa ggagcattca tgaacctgca tgcaaaggac aaaacccagc atcccgaaaa
    27961 cgtcgagctg ctcagtgcgc agaagccgat taccgacttt aagggtttgc tgaccaccat
    28021 tatttccgcc gtcgtctgtt tcggcattta ccacatcctg ccttacagcc ccgatgccaa
    28081 taaaggtatc gcgctgctga ttttcgttgc cgcactttgg ttcaccgagg ccgtccacat
    28141 cacggtaacc gcattaatgg tgccgattct cgccgtcgta ctcggtttcc ccgacatgga
    28201 catcaaaaag gcgatggctg atttttccaa cccgattatc tacatttttt tcggcggctt
    28261 cgcgcttgcc accgccctgc atatgcagcg gctggaccgt aaaatcgccg tcagcctgtt
    28321 gcgcctgtcg cgcggcaata tgaaagtggc ggttttgatg ttgttcctcg ttactgcctt
    28381 tctgtccatg tggatcagca acaccgccac cgccgcgatg atgctgcctc tagcaatggg
    28441 tatgctgagc cacctcgacc gggaaaaaga acacaaaacc tacgtcttcc tcctgctcgg
    28501 catcgcctat tgcgccagca tcggcggctt gggcacgctc gtcggctcgc cgcccaacct
    28561 gattgccgcc aaagccctaa atctggactt cgtcggctgg atgaagctcg acctgccgat
    28621 gatgctgttg attctgccct tgatgctgct ctccctgtac gtcatcctca aacctaattt
    28681 gaacgaacgc gtggaaatca aagccgaatc catcccttgg acgctgcacc gcgtcatcgc
    28741 gctgttgatt ttccttgcca cagccgccgc gtggatattc ggctccaaaa tcaaaaccgc
    28801 cttcggcatt tccaatcccg acaccgttat cgccctgagt gccgccgtcg ccgtcgtcgt
    28861 cttcggcgtg gcgcaatgga aggaagtcgc ccgcaatacc gactggggcg tgttgatgct
    28921 cttcggcggc ggcatcagcc tgagcacgct gttgaaaaca tccggcgcgt ccgaagcctt
    28981 gggacagcag gttgccgcca ccttttccgg cgcgcccgca tttttagtga tactcatcgt
    29041 cgccgccttc attatttttc tgaccgagtt caccagcaac accgcctccg ccgccctgct
    29101 tgtaccgatt ttctccggca tcgctatgca gatggggctg cccgaacaag tcttggtatt
    29161 cgtcatcggc atcgccgcat cttgcgcctt catgctgccg gttgccacgc cgcctaacgc
    29221 gattgtgttc ggcacgggct taatcaaaca acgagaaatg atgaatgtcg gcatactgct
    29281 gaacatccta tgcgtagtat tggttgctct gtgggcttat gctgtactga tgtaaaccat
    29341 cgacctaaac aacaagaccg tctgaaagaa tatttttcag acgaccttga agtttgtcgt
    29401 tcagacacaa tttgtcgaat aattcaaaac cggattctaa cgaaaggaaa acccatgatt
    29461 atcctgcaca ccaacaaagg cgacatcaaa atcgaactcg atttcgacaa agcccctgtt
    29521 accgccaaaa acttcgagca atacgtcaaa gacggcttct acgacggcgt aatcttccac
    29581 cgcgtcatca aaggcttcat gattcaaggc ggcggcatgg atgaaaacat gaacgaaaaa
    29641 gaaacccgcg atccgattca aaacgaagcg tccaacggcc tgcccaacga taaatacacc
    29701 atcgccatgg cgcgcacttc cgacccccat tccgccggcg cgcaattctt catcaacact
    29761 gccgacaacg ctttcctaaa cttccgttct aaagagctgt acggcaaaac cgtcgtccaa
    29821 gactggggct atgccgtatt cggtaaagtc gttgacggtt ttgacgttgt cgatgccatc
    29881 gaaagcgtat ccaccaaacg ccacggctac cacgacgatg tgccgaccga acctgtcatc
    29941 atcattaaag ccgaagcagt ataaaccggc aatccgaaag cagcctgcat aaaaggctgc
    30001 ttttatttca gacggcatgt tatttgatgt aaccgggcta tgccgtctga aaaccggacg
    30061 caagccttca gacggcatac cgctatgtta aaatacgccc gtttttccta aacggacaca
    30121 aaggaaacct tatggcaagc atcacccgcg acatcttcaa agcctacgac atccgtggca
    30181 tcgtcggcaa aaccctgacc gacgatgccg cttatttcat cggcagggcc atcgccgcca
    30241 aagccgccga aaaaggtatc gcccgcatcg cgctcggacg cgacggacgc ttgagcggcc
    30301 ccgaactgat ggagcacatc caacgcggcc tgaccgacag cggtatcagc gtactcaatg
    30361 tcggcatggt taccactcct atgctctact tcgcagccgt caacgaatgc ggcggcagcg
    30421 gagtgatgat taccggcagc cacaatccgc ccgattacaa cggtttcaaa atgatgctcg
    30481 gcggcgacac gctcgcaggc gaagccattc aagaactttt agctattgtt gagaaagacg
    30541 gttttgttgc cgccgacaaa caaggcagcg taaccgaaaa agacatctcc ggcgcatacc
    30601 acgaccacat cgtcggacac gtcaaactca aacgcccgat aaacatcgcc atcgacgcgg
    30661 gcaacggcgt gggcggcgcg tttgccggca aactctacaa aggtttgggc aacgaagtga
    30721 ccgaactttt ctgcgaagtg gacggcaatt tccctaatca ccaccctgat ccttccaaac
    30781 cggaaaacct gcaagatttg attgccgcgc tgaaaaacgg cgatgccgaa atcggcttgg
    30841 cgtttgacgg cgatgccgac cgcttgggcg tggttaccaa agacggcaac attatttatc
    30901 ccgaccgcca actgatgctg ttcgcccaag acgttttgaa ccgcaatccc ggcgcgaaag
    30961 tcattttcga tgtcaaatcc acacgcctgc ttgccccgtg gattaaagaa cacggcggag
    31021 aagccataat ggaaaaaacc ggccacagct tcatcaaatc cgctatgaaa aaaaccggtg
    31081 cactggttgc cggcgaaatg agcggacacg ttttctttaa agaacgctgg ttcggcttcg
    31141 acgacggcct gtatgccggc gcacgcctct tggaaatcct gtccgcctcc gacaatccgt
    31201 ccgaagtgtt ggacaacctg ccgcaaagca tttccacgcc cgaactcaac atctccctgc
    31261 ccgaaggcag caacgggcat caagttatcg aagaactcgc cgccaaagcc gaatttgaag
    31321 gcgcaaccga aatcatcacc atcgacggcc tgcgcgttga atttcccgac ggcttcggtc
    31381 tgatgcgtgc ttccaatacc acgccgattt tggtgttgcg ttttgaagcg gatacgcaag
    31441 cagccatcga gcgcattcaa aaccgattca aagccgtcat cgaaagcaat ccgcatttaa
    31501 tctggcctct gtaaaaatag aaaaaatgcc gtctgaaact ttcagacggc atttttcaca
    31561 agtagccaat caaatcttgg cagattggat ttggcttaaa aatctccgcg tccgttcgtg
    31621 tttgaggtgg tcgaacaact ctttcgggct gccctgctct acgataacgc cgccgtccat
    31681 cacgacgacg ttcgtggcaa cttccagcgt gaacttgatt tcgtgggtaa cgacgaccat
    31741 cgtccaacct tcccgcgcca attccttcat ggcgtccaac acgtcttgca ccaactcggg
    31801 gtccagcgcg gaagtgggtt cgtcaaacag catcaattca ggctgaatcg ccagtgcgcg
    31861 ggcgatacca acacgctgct gctgaccgcc ggaaagctgg tagggataca aatccacttt
    31921 atcgcccaag ccgacttttt ccagcagttt caaagcctct tcgcgcgctt gggcggcagg
    31981 cttgccctgt acggcaaccg gcccttccat cacgttttcc aacacggttt tatgcgggaa
    32041 gaggttgtat tgttggaata ccattccgga cttgcggcgc agtgccaaaa tatcgtgttt
    32101 gcttgttttt ttggaaaaat caatgcgtaa cggccgcgcg ttgtcgaact cgatttgtcc
    32161 gtcttcgggc atttccaacg cgtttaggca gcgcagaaat gttgttttac ccgagccgga
    32221 aggcccgagg atgacgacca cctgcccttt gcccacatcc aaatcgatgc cgcgcaaaat
    32281 ggtgttttcg ccaaaggtct tatggatatt gcggatttta atcatgacaa ctccttattt
    32341 ggcgacataa cggtcgaaac gtttttccaa acgcgcctga atcaaaaaca gcactttaca
    32401 gaaacaccaa taaaccaatg cagcttcgat atagacaggc aaaaagtcat aagtgcggtt
    32461 tgccgtttcc tgtgcgacac ggaaaagctc cgttaccgtt accacggcgg caagcgaggt
    32521 gtttttgaac aagccgataa actcgttgct caacggcgga acggcgacgc ggaatgcctg
    32581 cggtgcgacg atgcggcgga acgtctgcat ataggtcata ccgatggaga aacctgcttc
    32641 ccactgccct ttcggcacgg acaaaatcgc cgcgcgtatg gtttcggaag cgtatgcgcc
    32701 gacattgagc gaaaagccga tgatggcggc gggaatcgga ttgatataga tgccgacgga
    32761 cggcagcccg taaaacacaa tcacaagctg aaccaacagc ggcgtaccgc gaacgacgga
    32821 aatataaaat tccaccagct tcaacaagca tttttggaaa ataccgccgg aaggcatgat
    32881 tcttaccaaa gcaacggcta cggcaataat catgccgata acgaaagaag cgatcgccaa
    32941 aggcaaagac actgtaaagc cggctttaac catgggccaa aacgcgctga tgagcatatc
    33001 agcgcgtgtt tccgtcataa acggcagaga ggcaaggaaa ttattgaaca ctgatgtctt
    33061 ttccgaagaa ttgttcgccc agttttttca acgtgccgtc ggctttcagc tcgttgattg
    33121 ccgtgctgaa tttcgccacg gcctcgtcat tgcccttgtt gacaatcaga ccggaaccga
    33181 ctttttcatc ggcaggcgcg gaccacacga ttttcacccc cgcattcggg tttttcttca
    33241 gatagtccaa aaccgccaat tcatcgttca acgtcgcatc ggcgcgtttt tgttcaatca
    33301 gggtcagcga ttgcgccaaa ccgtccaccg gcacgagttg cgcacctgcg gctttggctt
    33361 tttcgccgta gttgctggtc agggattgcg cggttttcac gcctttgatg tcggctatgg
    33421 atttaatgtt gctgtcgtta cgcgcaacca aaaccgcacc gctccagctg taaggttcgg
    33481 atttgtcaaa tgtcgcctgg cgttcggggc tggtcaggcc gacttggttt gccaccacgt
    33541 cgaaacgtcc ggctttcaaa cccgccatca tcgaatccca ttgcgtttct ttaaactcga
    33601 cttttacgcc cagtttttcc gccacggcgc gggttacttc cacgtcgtaa ccggtcagtt
    33661 tgccgtcttt gtcgtggtag gtaaacggtg cgtaagtgcc ttccgtgccg acggtaaccg
    33721 tgcctttatt gttgatgcgc tcgattaaag aaccggaaat tgccgattgt gcaggcgcgg
    33781 aagatgctcc gctgccgcct tccgaaccgc cgcaggccgc caaaaccaat gcggcaatgc
    33841 cgccgagtac gaattttttc aacatcattc cctcctgtaa aaagtgtgca agtgcggcat
    33901 tctaagtgaa attgcggcaa acacaaagaa tacttttttt atatggttat tccaaatcgg
    33961 aatgggaaag atatgccgaa gccgccggaa gcggaaataa agggggcggg aaaggcggcg
    34021 ggcggcacga aaccgaaaag cggcatcagg tatgcaaacg gcggattcac gcacgccgtt
    34081 ccggcaaaaa tccgcctgcc gattcggtgc tataatccac ccgaaacaga tgacaccaaa
    34141 aaggaacatc tcatgaacac gccccaatcc gccatcattc ccgaccacgc ccaagccggc
    34201 atctttatcg aagccgactt cgccgccaac cgcctcaacg atattaaagc cgcctgccgc
    34261 gcttcgctcg acgcgttatc cgccttgaaa gcccgctttc cggacgatat tttgggtctg
    34321 accatcgcct tcggcagcaa agcttgggca acattcggac acacggacga aggcagtgaa
    34381 atcaaaccct tccccgaaat gggcaacggg cttgcgccgt ccacgcagca cgatatgtcc
    34441 atccacatcc aatccttccg ccaaaacgca gcctacgcgc ttgcccaatc cgttttgggc
    34501 gcgttcggcg acagcatatg cgtcgcgtcc gaagaacacg gtttgcgcct gtatcaggat
    34561 cgcgggctgg acggtttcgt cgacggcacg gaaaacccgc aaggcgatga aacaatccgc
    34621 gaagtcgcca tcatccccga agggctgccc gatgcaggcg gcagctatgt cctgctgcaa
    34681 aaatacctgc acgatttgaa aaaatgggat gccgtccccg tcgccgaaca ggaggcctcg
    34741 gtcggacgca gcaaggaaac cgacgacgaa ttcagccgcg atgtccgcct gcccgattcg
    34801 cacctcggcc gcgtcaacct gaaagaaaac ggagtcggcc tgaaaatcgt ccgccgcagc
    34861 ctgcccttcg gcaaaatcag cggcgaacac ggtttgatgt ttaccgcata ctgccgcacg
    34921 ctgcataata tcgaagcgca attattgagt atgttcggcg acacggacgg caaaaccgac
    34981 ctgctgctcc gacacctctc cgccgccgtt tcgggcggat actactacgc gccgtccgtc
    35041 gaacgcctgc aaaacctcta agccggacga tgccgtctga aacccttttt cagacggcat
    35101 tttcccgttt taccgtccaa ccgttttcat acacgctatg tccgcaccca tccgagcatt
    35161 cgacccgctg accgccccca tttccggcac caacctgatt gaggcctctg ccggcaccgg
    35221 caaaacctac ggcattgccg ccctgtttac gcgcctgatc gtattggaac aaaaaagcgt
    35281 cgagcgcgta ttggtcgtta ccttcaccaa agccgccact gccgagctga aaacacgcct
    35341 gcgcgcgcgt ttggacgatg tgttgcaggt tttggaaagc aaagaaattg ccgaacttgg
    35401 agacgacacg ctttcagacg gcattgccgc ctactgcgcc gaacaccacg aaggcgacac
    35461 cttcctgccc gcactcttgg aacaggcttt gcaaaaagaa agccggacgc gtctgattgt
    35521 ccgcctcaag gccgccatcg ggcaattcga caacgccgcc atctacacca tccacggctt
    35581 ctgccagcgc atcctgcgcg actacgcctt cctgtgccaa gcaccgttcg atgtcgaact
    35641 gaccgaagaa gacggcgacc gcctgcttgt cccggctcaa gatttttggc gggaacgcgt
    35701 cagcggcgac ccggtgcttg ccgcattggc gtttaaacgc aaagctgtgc cgcaaaccgt
    35761 ccttgcccaa atccgcgcct acctgtcccg cccgtacctg aatttccgcc gtccgcaggc
    35821 ggatttgaaa caggcgcagc gcgacgccga aacctcatgg caaaccgtct gccgcctgct
    35881 gcccgagctg gaagccggct tttggcgcat ccatcccgac ctcaacggca acagttaccg
    35941 caaaaacagc ttcggcaacc tttttaaaga actggcacaa aaatccgccg ccggacaact
    36001 gccctttctg gacaaagaca cgcacgaacg gctcttgaaa ctctcatccg acaaactcga
    36061 agccggactg aaaaaaggca aaacgcccga tgcggcagta tttgccgaat tgcagaaact
    36121 ggcagacttc gggcgcgatt tgaacgcact cgaagaagcg gaagaagcaa caatgatccg
    36181 gctgcaactg gatttaatcg aatatctcaa cagcagcctt gccgaaatga aaaaatcgcg
    36241 ccgcgaacgc ggtttcgacg acctgctgct cgatgtccac accgcgctga ccgacaatcc
    36301 gcacgccgaa acccccgccc gcgccgttgc cgaaaactgg gaaatcgcgc tgatcgacga
    36361 gttccaagac accgacccgc tgcaatacga aatcttccaa aagattttca tcgcccgaaa
    36421 ccgcccgctg ttcctcgtcg gcgaccccaa acaggcgatt tacagctttc gcggtgcgga
    36481 catttacgcc taccttcagg cggcggaaga cgcgcggcac cgctacacgc tcgccaccaa
    36541 ctaccgcagc cacgccgcgc ttatcggcag catcggcgcg ctgttccgcc tcaaagaacg
    36601 cccgttcgtt ttggaaaaca tcggctattc ggaagtcggt gcggcgcgtg ccgaaagcag
    36661 gttgtcgccc aaacgtcctg ccgtacagct ccgttggctg cacgaaaacg acaatgaaaa
    36721 agccaacaaa gacgttttgc gccgccgtgc cgccgactat tgcgccgacg aaatcgcccg
    36781 cgcgctcaac gaagcctccg gaggccgtct gaatttcaaa ggccgcccgt tgcagtcggg
    36841 cgatattgcc gttctggtac gcacgcacaa cgaggcggtg atgatttccg ccgcactgaa
    36901 aaaacggcag gtgcaaagcg tcctgctttc gcgcgaatcc gtgttcgcct cgcccgaagc
    36961 cgccgccctg tccgcgctca tcggcttctg gctcgaaccg cgccgcgccg gaacgctgcg
    37021 ctttgtcctg acaagcggca tattcggtta tgacgcgcag caattgcacg acttcaacca
    37081 aaacgaaagc gagattttgc attgggcgga atctgcccga accgcgctcg acatatggca
    37141 aaaatacggc attttcgccg ctatgcagca gttttcccaa acacacggca tcgaaacgcg
    37201 cctcttaagc cggaacaacg ggcgcagcct gaccaattat ttccaactgc tcgaactgct
    37261 tgccgccgaa gacgcgcaaa accgcaaccc cgccgcactg cacaaatggc tgcgcgatca
    37321 aatcagcctg gccggcaaca acggcggcga caaccgcgcc atccgtttgg aaagcgacga
    37381 agatttggtc aaaatcgtta ccatgcacgc ctcgaaaggt ttgcagtatc cgctggtgta
    37441 ctgcccgttt gcgtgggacg cgcaagatac cggaccgtcc gactggcaaa tcctccacca
    37501 aggcgcaaac cgggccgaac ttttggcaaa ggcgcaactg tcggaaagcg aacagaagca
    37561 atacgccgac gaagaaatgg cggaacgcct gcgcctgctt tatgtcgcgc tgacgcgtgc
    37621 cgaggaacag ctcaacatct acgccgccta ttcttccgat accgccgaca acccccttgc
    37681 ctacctgatc gaaggcttgc cgcaagacag ccgcgaaacc gtccgccgta cctatgcgtg
    37741 tgaaaaagac ggcataacga tgctcaaacg caactggcgg cgcgtggcgg acaatgcccc
    37801 ggccggcacg gatttcgcct tcacagaaga tgcgccgccc cctgccgcct atcgcggcaa
    37861 cgccgatcaa gccgccgaat ttgccgcaaa cagcattccc gaacgcggat tccgatttgt
    37921 ccgccacacc agcttcaccg ccttaagccg ccaaacccaa acgcccgacg gcggcgaaga
    37981 agatgcctgc ccgtccttgg atgccgccga aacctcggtg ccggcgatgc cgtctgaaac
    38041 gccgacggct tcagacggca tatcgataca cgactttccg aaaggcacgc aggcggggct
    38101 gtgcctgcac gaaattcttg aagatttcaa attcggacaa gcagccgccg aacaggaaac
    38161 cctcattgcc gacaagctga aaaaatacgg ttttgaagaa atatggctgc ccgccgttgc
    38221 cgaaatggcg gaagcctgcc gcaaaacgcc gctgactggg gcatacggcc tgtccgacat
    38281 cccgcccggg tgccgccgtc ccgaaatggg ctttaccctc cacaccgaag acttcggcct
    38341 caaacggctg cgcgactggc ttgcccgcga cgacatcagg ctgcccgaag tctgccgtgc
    38401 cgccgccgag acgctcgact tccacaccgt caacggcttt ttaaacggct ttatcgatat
    38461 ggtctgccaa gaccccgacg gcaatatctg catcatcgac tacaaatcca accacctatc
    38521 cgcctacacg cggcaggcga tggacgaagc cgtcgcgcac cagcactatt accttcaggc
    38581 actgatttac gccgttgccg ccgcgcgcta cttcaaactg cgcggacaac cgcccgccgc
    38641 cgtttccgtc cgctacctgt ttttgcgcgg attggatggc aaaggcggcg gcgtttggcg
    38701 ttgggacata gatgccgccg ctttggaaca gataaaataa cttcccgtcc cttaagacga
    38761 caaccgcagg agaaccctat gaatatcaaa caattgatta ccgccgcact cattgcctca
    38821 gccgcctttg ccacgcaggc agccccgcaa aaacccgtat ccgccgccca aaccgcgcaa
    38881 cattcagccg tttggatcga tgtccgttcc gaacaggaat ttagcgaagg tcatttgcac
    38941 aacgcggtca acatccccgt cgaccaaatc gtccgccgca tatacgaagc cgcgcccgac
    39001 aaagacacgc cggtcaacct ctactgccgc agcggacggc gtgccgaagc cgcccttcaa
    39061 gagctgaaaa aagcaggtta tacaaatgtt gccaatcacg gcggttatga agacctgctc
    39121 aaaaaaggga tgaaatgacg caatgccgtc tgaaaggaca ggctttcaga cggcatacgg
    39181 cgttccggca cgcggcacgg catcaggcgc ggcgcgtgcc gccccgtccg aagcccgata
    39241 aacaaaccga taaggaaata cgatgaaaca catactcccc ctgatcgccg catccgcact
    39301 ctgcatttca accgcttcgg cacaccctgc cgacaaaccg cccacccaaa acgaaaccgc
    39361 tatgaccacg cacaccctca cctcgaaata cagttttgac gaaaccgtca gccgccttga
    39421 aaccgccata aaaagcaaag ggatggacat ttttgccgtc atcgaccatc aggaagcggc
    39481 acgccgaaac ggcctgacca tgcagccggc aaaagtcatc gtcttcggca cgcccaaggc
    39541 cggtacgccg ctgatggtca aagaccccgc cttcgccctg caactgcccc tgcgcgtcct
    39601 cgttaccgaa acggacggca aagtacgcac cgcctatacc gatacgcgcg ccctcatcgt
    39661 cggcagccgc atcagtttcg acgaagtggc aaacactttg gcaaacgccg aaaaactgat
    39721 acaaaaaacc gtaggcgaat aagcccgtcg ccgacatatg gcggatggaa atcaaatgca
    39781 cacgcccctc attcccccga agtcgggaat ccaagcggtt gggttaccgt gatttccaat
    39841 cattgccgaa acggacgatt ccggattccc gccttcgcag ggatgacggg cgtatgcgtt
    39901 ttgccatatc acaggcaact ttgccgcaaa tcttatctgt cgccggtatg ccttcttgtt
    39961 tcaatccgcc acaaaccaaa tgccgtctga aacctgattt cagacggcat ttccgtttat
    40021 tccctgctgt cgcggcaaat atcgatggct tcctgcaaac ttgaaacgcc gtggattttc
    40081 aggttcggaa actctttggc attgcgcggc atattggctt tggggacgat ggcgcgtttg
    40141 aagccgagct tttccgcttc tttgagccgc tcttgtccgc gtgcgacggg gcggacttcg
    40201 ccgcttaggc cgatttcacc gaagaccacg gttttttcag gcatagggcg gttgcggaag
    40261 ctggaaagca tcgcgaggat gaccgccaaa tccgccgccg gttcgccgat tttcacgccg
    40321 ccgacggcgt ttaaaaaaac atcctgatcg aaacaggcga tgccgccgtg ccggtttaac
    40381 acggcaagca gcatcgcaag gcggttttgt tccaatccga cgctgaggcg tttgggtgtg
    40441 aagccgtgcg cgtcatcgac caatgcctga atttcgacca aaagcgggcg gctgccttcc
    40501 tgtgtaacca aaacgcacga accgggcgtg tcgtcgcggt agctggcgag gaagatggcg
    40561 gacgggttgg atacgccttt caaaccgttt tcggtcatcg cgaacacgcc caattcgttt
    40621 gccgcgccga aacggttttt gatggcgcgt atcatgcggt agttggaatg ttggtcgccc
    40681 tcgaaataca gcacggtatc gaccatatgt tccagcacgc gcgggccggc aatcgctccg
    40741 tctttggtaa cgtgtccaac cagtatcatg gcgataccca tttgtttcgc catacgcgtc
    40801 agttgggcgg cacattcgcg cacctgcgac acggaaccgg gggcggacgt gatttggtcg
    40861 gaatacatgg tttggatgga atcgataacg acgacttcgg gctgatgctg tttcaaggcc
    40921 gcctgaatcg cttccatgcg gatttcggca agcaggttta cgccttcggc gtgcagttcc
    40981 aaacgttgcg agcgcagggc gacctgttgg gcggattctt cgccggaaac gtacagcact
    41041 ttgcggcttt gcgccatttt ggcgatggtt tgcaacaaca gcgtggattt gccgatgccg
    41101 ggatcgccgc cgagcaggat gaccgcgcca tcgaccaaac cgccgcccaa tacgcggtcg
    41161 agttcgccca taccggtcgg attgcgcggc acttcggcag cggtaacggc ggagagggat
    41221 tggacggtcg aagtgtccgc cgcccaagat tggaatcggg cgtttttcgg ctcgggcgcg
    41281 gcaaagcttt cctgaagcgt gttccactcg ccgcaatgcg gacatttgcc ctgccatttc
    41341 ggggaagtgc cgccgcattc ggtgcattgg taaagggttt taagcgtttt tgccatgatg
    41401 ttttccctat gggatttttg aagtgtaaaa aaatcgtctg aaacttcttt tctaaaaaat
    41461 tttttattat tagtttgtta attttgatta caagaccata tcacgataca aacaagggtg
    41521 gctttctgtt attttgcggc ataatgacgg aaaatacaaa acaggaatcc caaacaatga
    41581 ctttcccatt aaatataatg gtagaatt
//
LOCUS       DAXFZR010000007        40794 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000007 DAXFZR010000000
VERSION     DAXFZR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40794
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.007"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..217)
                     /locus_tag="ACFJAS_000274"
     CDS             complement(<1..217)
                     /locus_tag="ACFJAS_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3981633.1"
                     /translation="MKITHCKLKKEVQKEPLRSFVPEVTARSAADILGIHPDSAALFY
                     RKIRTVTNHRLALAADEVFECPAGPGGS"
     gene            455..1798
                     /locus_tag="ACFJAS_000275"
     CDS             455..1798
                     /locus_tag="ACFJAS_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC3981634.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEKVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1801..3033
                     /locus_tag="ACFJAS_000276"
     CDS             1801..3033
                     /locus_tag="ACFJAS_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC3981635.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            3026..3802
                     /locus_tag="ACFJAS_000277"
     CDS             3026..3802
                     /locus_tag="ACFJAS_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC3981636.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKDAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3802..4428
                     /locus_tag="ACFJAS_000278"
     CDS             3802..4428
                     /locus_tag="ACFJAS_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC3981637.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4432..5025
                     /gene="nqrE"
                     /locus_tag="ACFJAS_000279"
     CDS             4432..5025
                     /gene="nqrE"
                     /locus_tag="ACFJAS_000279"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC3981638.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            5039..6256
                     /gene="nqrF"
                     /locus_tag="ACFJAS_000280"
     CDS             5039..6256
                     /gene="nqrF"
                     /locus_tag="ACFJAS_000280"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC3981639.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVNEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6773..7828
                     /locus_tag="ACFJAS_000281"
     CDS             6773..7828
                     /locus_tag="ACFJAS_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC3981640.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGETEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7854..8069
                     /gene="nqrM"
                     /locus_tag="ACFJAS_000282"
     CDS             7854..8069
                     /gene="nqrM"
                     /locus_tag="ACFJAS_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC3981641.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(8193..8771)
                     /gene="grpE"
                     /locus_tag="ACFJAS_000283"
     CDS             complement(8193..8771)
                     /gene="grpE"
                     /locus_tag="ACFJAS_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC3981642.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVARKEA"
     gene            8954..9772
                     /gene="cysE"
                     /locus_tag="ACFJAS_000284"
     CDS             8954..9772
                     /gene="cysE"
                     /locus_tag="ACFJAS_000284"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC3981643.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9976..11487)
                     /gene="ubiB"
                     /locus_tag="ACFJAS_000285"
     CDS             complement(9976..11487)
                     /gene="ubiB"
                     /locus_tag="ACFJAS_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC3981644.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11522..11979)
                     /locus_tag="ACFJAS_000286"
                     /pseudo
     CDS             complement(11522..11979)
                     /locus_tag="ACFJAS_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(11976..12122)
                     /locus_tag="ACFJAS_000287"
     CDS             complement(11976..12122)
                     /locus_tag="ACFJAS_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981645.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(12139..12477)
                     /gene="erpA"
                     /locus_tag="ACFJAS_000288"
     CDS             complement(12139..12477)
                     /gene="erpA"
                     /locus_tag="ACFJAS_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC3981646.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12610..13296)
                     /locus_tag="ACFJAS_000289"
     CDS             complement(12610..13296)
                     /locus_tag="ACFJAS_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3981647.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTV"
     gene            13483..13755
                     /locus_tag="ACFJAS_000290"
     CDS             13483..13755
                     /locus_tag="ACFJAS_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981648.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDTEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            13963..15891
                     /gene="dnaK"
                     /locus_tag="ACFJAS_000291"
     CDS             13963..15891
                     /gene="dnaK"
                     /locus_tag="ACFJAS_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC3981649.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            16114..18040
                     /locus_tag="ACFJAS_000292"
                     /pseudo
     CDS             16114..18040
                     /locus_tag="ACFJAS_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            18097..19074
                     /locus_tag="ACFJAS_000293"
     CDS             18097..19074
                     /locus_tag="ACFJAS_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFC3981650.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLTAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            19411..19548
                     /locus_tag="ACFJAS_000294"
     CDS             19411..19548
                     /locus_tag="ACFJAS_000294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981651.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            19627..20396
                     /locus_tag="ACFJAS_000295"
                     /pseudo
     CDS             19627..20396
                     /locus_tag="ACFJAS_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20423..21115
                     /locus_tag="ACFJAS_000296"
     CDS             20423..21115
                     /locus_tag="ACFJAS_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3981652.1"
                     /translation="MLCLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            21308..21922
                     /locus_tag="ACFJAS_000297"
     CDS             21308..21922
                     /locus_tag="ACFJAS_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC3981653.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSRWIMAVIYIGLGLFIIIENNTIRTIISII"
     gene            22218..23313
                     /locus_tag="ACFJAS_000298"
                     /pseudo
     CDS             22218..23313
                     /locus_tag="ACFJAS_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(23360..25549)
                     /locus_tag="ACFJAS_000299"
     CDS             complement(23360..25549)
                     /locus_tag="ACFJAS_000299"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC3981654.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTGQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRALLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            25689..26474
                     /locus_tag="ACFJAS_000300"
     CDS             25689..26474
                     /locus_tag="ACFJAS_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC3981655.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(26573..28507)
                     /locus_tag="ACFJAS_000301"
     CDS             complement(26573..28507)
                     /locus_tag="ACFJAS_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC3981656.1"
                     /translation="MSLIECKNINRCFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(28573..29751)
                     /locus_tag="ACFJAS_000302"
     CDS             complement(28573..29751)
                     /locus_tag="ACFJAS_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC3981657.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQAAYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLDMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAVEREIRTGMKDSMNTEVKSGLKEGDKVVISEITAAEQQESGERVMGGPPRR
                     "
     gene            29977..30366
                     /locus_tag="ACFJAS_000303"
     CDS             29977..30366
                     /locus_tag="ACFJAS_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC3981658.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFQTYDKNKLKQNEYFKITLSEVSPDHFTLQAVPNTATNE
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            complement(30460..33945)
                     /gene="smc"
                     /locus_tag="ACFJAS_000304"
     CDS             complement(30460..33945)
                     /gene="smc"
                     /locus_tag="ACFJAS_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC3981659.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            34330..34998
                     /locus_tag="ACFJAS_000305"
     CDS             34330..34998
                     /locus_tag="ACFJAS_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981660.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALGGNSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAVALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            34970..35455
                     /locus_tag="ACFJAS_000306"
     CDS             34970..35455
                     /locus_tag="ACFJAS_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFC3981661.1"
                     /translation="MSPMARKIYDAVNVRQLNRLSKRTNRVGASAAALASLKPAQLGK
                     NDKFAFSLGFGSYKNAQAVAMGAVFKPAENVLLNVAGSFAGPNRAFGAGVSWKFGGKP
                     TPAVAAQNAAHPAKVLQLRQEVAALRARQAETDRKLHKQAEMENELQQLRRALSELKK
                     H"
     gene            35664..36386
                     /gene="lpxH"
                     /locus_tag="ACFJAS_000307"
     CDS             35664..36386
                     /gene="lpxH"
                     /locus_tag="ACFJAS_000307"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC3981662.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            36655..38241
                     /locus_tag="ACFJAS_000308"
     CDS             36655..38241
                     /locus_tag="ACFJAS_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC3981663.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(38407..38610)
                     /locus_tag="ACFJAS_000309"
     CDS             complement(38407..38610)
                     /locus_tag="ACFJAS_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC3981664.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            38726..39223
                     /locus_tag="ACFJAS_000310"
     CDS             38726..39223
                     /locus_tag="ACFJAS_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC3981665.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRHWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            39305..40495
                     /locus_tag="ACFJAS_000311"
     CDS             39305..40495
                     /locus_tag="ACFJAS_000311"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC3981666.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKV"
     gene            complement(40496..>40794)
                     /locus_tag="ACFJAS_000312"
     CDS             complement(40496..>40794)
                     /locus_tag="ACFJAS_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3981667.1"
                     /translation="VCQNTVAGVFFEARFQQCLLPALAQKSVIISDNARFRRMGALRG
                     TAEKLGHKVLPPAPYSPEPNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         9791 a        11220 c        10243 g         9540 t
ORIGIN      
        1 agcttccgcc cggcccggcg gggcactcga aaacctcatc ggcagccaag gccaaacgat
       61 ggttggtaac cgtgcggatt ttacggtaaa acagtgccgc cgaatcggga tggataccca
      121 aaatatcggc tgcagaacgg gcggtaactt ccggtacaaa agaacggagc ggttcttttt
      181 gtacttcttt ctttaatttg cagtgtgtta tcttcatatt tcgagggtaa catatctgct
      241 aatctagtac agacccttat caaatcaata tcccccttcc gacaattaaa aacggcttcc
      301 gcaaggaaaa aagcggcaaa tctgacaaac aggtacaatt cctgataaaa tctaacactt
      361 tgctacatta atttattcaa tgcttttgat gccacgcgac acggtatcaa aaacacgccg
      421 tttttacctt aaccatcaca agtaatgcaa atctatgatt aaaatcaaaa aaggtctaaa
      481 tctgcccatc gcgggcagac cggagcaagt catttatgac ggcccggcca ttaccgaagt
      541 cgcgttgctt ggcgaagaat atgtcggcat gcgcccctcg atgaaaatca aggaaggtga
      601 agccgtcaaa aaaggccaag tgctgtttga agacaaaaag aatccgggcg tagtatttac
      661 tgcgccggct tcaggcaaaa tcgccgctat tcaccgtggc gaaaagcgcg tacttcagtc
      721 agtcgtgatt gccgttgaag gcaacgacga aatcgagttc gaacgctacg tacctgaagc
      781 gctggcaaaa ttgagcagcg aaaaagtgcg ccgcaacctg attcaatcag gcttatggac
      841 tgcgcttcgc acccgtccgt tcagcaaaat ccctgccgta gatgccgagc cgttcgccat
      901 cttcgtcaat gcgatggaca ccaatccgct ggctgccgac cctacggtca tcatcaaaga
      961 agccgccgaa gacttcaaac gcggcctgtt ggtattgagc cgcctgaccg aacgtaaaat
     1021 ccatgtgtgt aaagcagcag gcgcagacgt gccgtctgaa aatgctgcca atatcgaaac
     1081 acatgaattt ggcggcccgc atcctgccgg cttgagtggc acgcacattc atttcatcga
     1141 gccagtcggc gcgaataaaa ccgtgtggac catcaattat caagacgtga ttgccatcgg
     1201 acgtttgttc gtaacaggcc gtctgaatac cgagcgcgtg gttgccttgg gcggcctgca
     1261 agtcaacaaa ccgcgcctct tgcgtaccgt tttgggtgcg aaggtgtctc aacttaccgc
     1321 cggcgaattg gttgacgcgg acaaccgcgt gatttccggt tcggtattga acggtgcgat
     1381 tgcacaaggc gcgcatgatt atttgggacg ctaccacaat cagatttccg ttatcgaaga
     1441 aggccgcagc aaagagctgt tcggctgggt tgcgccgcag ccggacaaat actccatcac
     1501 gcgcaccact ctcggccatt tcctaaaaaa caaactcttc aagttcacga cagccgtcaa
     1561 cggcggcgac cgcgccatgg taccgatcgg cacttatgag cgcgtaatgc cgttggacat
     1621 cctgcctacc ttgcttttgc gcgatttaat cgtcggcgat accgacagcg cgcaggcttt
     1681 gggttgcttg gaattggacg aagaagacct cgctttgtgc agcttcgtct gcccgggcaa
     1741 atacgaatac ggcccgctgt tgcgcaaagt gctggaaacc attgagaagg aaggctgatt
     1801 atgggcttaa aacattttct ggaaaaaatc gaaccgcact tcctgccggg cggcaaacat
     1861 gaaaaatggt atgccctcta tgaagctgcg gcgacgattt tctatacatc cggcgcggta
     1921 acgcgcaaag cggcgcacgt ccgcgacgcg ctcgactcca aacgcatgat gattttggtg
     1981 tggctggctt tgttccccgc catgttttac ggcatgtaca acgtcggcgc acaggcattc
     2041 ggtgccttaa cgcccgattt gctgcaacaa agcatcgcca acgactggca ttacgccctt
     2101 gccaacgctt tgggcatcaa tatgtcgcct gaagcgggcg tgttgggcaa aatgctgttc
     2161 ggcgcgattt acttcctgcc gatttacgcg accgtattta ttgtgggcgg cttctgggaa
     2221 gtcttgttcg catccgtacg caaacacgaa atcaacgaag gtttcttcgt tacttcgatt
     2281 ctgtttgcct taatcgttcc gcccacgctg ccgctgtggc aggcggcttt gggtatttct
     2341 ttcggcgttg tggttgcgaa agaagtattc ggcggtacag gtaaaaactt catgaaccct
     2401 gcgctggcag gccgcgcctt cctgttcttc gcctaccccg ccaacttgag cggcgatgcg
     2461 gtttggacgg cggttgacgg ctattccggc gcaaccgcgc tggcgcaatg ggcggcacac
     2521 ggtgcagacg gcctgaaaaa cgccgtaacc ggtcaaacca tcacttggat ggacgcgttt
     2581 atcggcaaac tgcccggctc catcggcgaa gtctccactt tggcactctt aatcggcggc
     2641 gcgtttatcg tgtttgcccg catcgcttct tggcgcatta ttgccggcgt gatgatcggt
     2701 atgattgcga tgtcttcgct gtttaacttc atcggttctg acaccaacgc tatgtttgct
     2761 atgccttggt actggcactt ggtggtcggc ggcttcgcca tcggtatgct gtttatggcg
     2821 accgaccccg tttccgcttc ctttaccaat gtcggcaaat ggtggtacgg cgcgcttatc
     2881 ggcgtaatgt gcgtgttaat ccgcgtggcc aatccggctt accccgaagg catgatgttg
     2941 gcgattctgt ttgccaacct gtttgccccg attttcgact atttcgtcgc acaagcgaac
     3001 atcaaacgca gaaaggcgcg cagcaatggc taagaaattc gataaagaca gcttcagcgg
     3061 cacgctgatt gtcgtgttgg cggtcagcct gatttgctcg gtcatcgttg ccggtgcggt
     3121 cgtcggcttg aaacccatcc aagaaaaaca aaaactccaa gacaaacaag gctatatttt
     3181 gagcgtggcc ggtttgatgg ataaggacac cgacatcggt aaaacctttg ccgagcgtat
     3241 cgagcaacgc gtggtcgatt tggcgaccgg cgaatatgtg aaagacgcgc cgaaagactt
     3301 cagcgcgcgc atcgcaggca aagaccccgc gcaaagcatc cgcatcaaac ctgaagacga
     3361 tttggcaggc atcaaaagcc gcgccaaata caccgaggtt tatttggtaa aaggcgaaga
     3421 cggcaaaatc gggcaaatca tcctgccgat gcacggcaac ggtttgtggt cggtgatgta
     3481 cggttttgtc gccatccgac ccgacggcaa caccatcaac ggcattacct attacgagca
     3541 aggcgaaacc ccgggcttgg gcggcgaaat cggcaaccct ctgtggcagc aaaaattcgt
     3601 cggcaaaaaa ttgtttgacg gacaaggcaa actcgccctg cacgtgggca aagacgcggg
     3661 ttcggacaaa gaacacggcg tagacgccct ctccggcgcg tcgctgactt ccaaaggcgt
     3721 gcaaggttcg ttcgcctact ggttcggcga aaacggctat atcccctacc tgaacaaatt
     3781 gaaatcagca ggagtgcaat aatggctgat atgaaacgct tgaaacattt gatgttctca
     3841 ccctttatcg acaacaaccc gattgccttg caggttttgg gtatttgttc ggctttggcg
     3901 gttaccacca aacttcagac ggccatcgta atgggtattt ccgtagcttt ggtaaccggt
     3961 ttttccagct tcttcatttc gctggtgcgc aactacatcc ccaacagcat ccgcatcatc
     4021 gtgcaaatgg cgattatcgc gtcgctggtt acgctggtcg accaactgct tcaggcattt
     4081 gcctacgaat tgtccaagca gctttccgta ttcgtcggcc tgattattac caactgtata
     4141 gtgatgggcc gtgccgaagc atttgcgatg aaagagccgc cgctggaaag cctgatcgac
     4201 ggcatcggca acggtgcggg ctacgggatg atgctgcttg tcgtcgccac cgtccgcgaa
     4261 ctgatcggct caggcaaact tttaggttat accgtctttc aaaccgtaca ggacggcggc
     4321 tggtatcaga ccaacggcct cttcctactc gcccccagcg cgttcttcat catcggcttt
     4381 ttgatttggg gactgcgtac ttggaaaccc gaacaggcgg aggaataagc tatggaacac
     4441 tatttgagcc tcttcatcaa atccgtcttc attgaaaata tggcgctatc cttctttttg
     4501 ggtatgtgta cgtttttggc ggtatccaaa aaagtatcca ccgcattcgg tttgggtgtg
     4561 gcggtaattt tcgtactcgg gctgtccgtc cccgccaacc aattggttta ctcgctgctc
     4621 aaagacggcg cgattgtcga aggcgtggat ttgacctttt tgaaattcat cacctttatc
     4681 ggcgtgattg ccgctttggt gcagattttg gaaatgttct tggacaaatt cgtccccgcc
     4741 ctctataacg cgctgggcat ctacctgcct ctgattaccg taaactgcgc gattttcggc
     4801 gccgtatcgt ttatggcgca acgcgaatac gacttcggcg agtccgtcgt gtacggcttc
     4861 ggcgcgggtt tgggctggat gttggcgatt gtcgctttgg cgggcattac cgaaaaaatg
     4921 aaatattcgg acgctcccaa aggcctcaaa ggcttgggca ttacctttat cgccgccggc
     4981 ctgatggcaa tggcgtttat gtcgttctcc ggcatccagt tataagaaag ggaccggtat
     5041 ggagattatt ttaggtatcg tgatgtttac cgtcatcgtc ttagctttgg cactgatgat
     5101 tctgtttgcc aaatccaagt tggtcagcga aggcgacatc accatcaaag tcaatgatga
     5161 aaaagaactg actatgcccg ccggcggcaa actcttgggc gcgcttgcca gccaaggcat
     5221 ctttgtcccc tccgcctgcg gtggcggcgg ttcgtgcgga caatgccgcg ttgtcgtgaa
     5281 aagcggcggc ggcgacattc tgccgaccga gctgtcccac atcagcaaac gcgaagcacg
     5341 cgaaggctgc cgtttgtcct gccaagtcaa tgtcaaaacc gacatggaca tcgaagtacc
     5401 cgaagaagtg ttcggcgtga aaaaatggga atgcaccgtc atttccaacg acaacaaagc
     5461 aacgttcatt aaagaactca agcttgccat tcccgaaggc gaagaagttc ctttccgcgc
     5521 cggcggctac attcaaatcg aagccccgcc gcacaccgtt gcctacaaag acttcgacat
     5581 tcccaaggaa tatcacgaag actgggacaa atacaatctg tggcaatacg tttccaaagt
     5641 gaacgagccg attttgcgcg cctactctat ggcttcgtat cctgaagaaa aaggcatcat
     5701 tatgctgaac gtgcgtatcg ccacgcctcc cccgcgcgta cccgatgcgc ctccgggaca
     5761 aatgtcgtcc tacatctggt cgctcaaacc cggcgacaaa gttacgattt ccggcccgtt
     5821 cggcgaattt ttcgccaaag acaccgatgc cgaaatggta tttatcggcg gcggtgcggg
     5881 tatggccccg atgcgttccc acattttcga ccagttgaaa cgtttgcact ccaaacggaa
     5941 aatcaccttc tggtacggcg cacgctccaa acgcgaaatg ttctatgtcg aagacttcga
     6001 ccaactcgcg gcagaattcc cgaacttcac ttggcacgtc gccctgtccg acccattgcc
     6061 tgaagacaac tgggacggct acacgggctt catccacaac gtggtttacg aaaaccacct
     6121 gaaaaaccac gaagcaccgg aagactgcga attttatatg tgcggcccgc cgattatgaa
     6181 ccagtccgtc atcaaaatgc tcaaagactt gggcgtggaa gacgaaaaca tcctcttgga
     6241 cgatttcggc ggttaaatat tcggcttcaa acacaaaagg aagaaacccc ggtttcttcc
     6301 ttttttatcc gatgccgtcc gaacaccctt cagacggcat ccgcccatac ccgtatcaat
     6361 atcgtttttt taccctgcgg cggatgtgcg gctaaatatt ttgacgaaaa aacaaagaag
     6421 tatacttctt tttagttatg gttgattcca taactaaaaa agtatcgatc aacccatttt
     6481 acgaggtacg gataatgtct gacagctttg aaaacagccc cgaatacgat gattggattg
     6541 aatcgggcgg tcgtgatgaa gattacgaat actactacaa caaatggcaa cgcaggacgc
     6601 gctgaccatt aaaccctgac aaaccggttt gtaaaacacc ggtttgtttt ttattaccgc
     6661 cacccaaacg acgggcgatg ccgcccgaac gcccttcaaa cggcatcggc atagaaacat
     6721 accggccgtt ttgcgatata attcaagcca tttgccaaaa ccgtcaaaca ctatgccgtc
     6781 tgaaacacgc ctgccgaacc ttatccgcgc cttgatattt gccctgggtt tcatcttcct
     6841 gaacgcctgt tcggaacaaa ccgcgcaaac cgttaccctg caaggcgaaa cgatgggtac
     6901 gacctatacc gtcaaatacc tttcaaataa tcgggacaaa ctcccctccc ctgccaaaat
     6961 acaaaagcgc attgatgatg cgcttaaaga agtcaaccgg cagatgtcca cctaccagac
     7021 cgattccgaa atcagccggt tcaaccaaca cacagccggc aagcccctcc gcatttcaag
     7081 cgatttcgca cacgttaccg ccgaagccgt ccgcctgaac cgcctgactc acggcgcact
     7141 ggacgtaacc gtcggccctt tggtcaacct ttgggggttc ggccccgaca aatccgttac
     7201 ccgtgaaccg tcgccggaac aaatcaaaca ggcggcatct tatacgggca tagacaaaat
     7261 cattttgcaa caaggcaaag attacgcttc cttgagcaaa acccacccca aagcctattt
     7321 ggatttatct tcgattgcca aaggcttcgg cgttgataaa gttgcgggcg aactggaaaa
     7381 atacggcatt caaaattatc tggtcgaaat cggcggcgag ttgcacggca aaggcaaaaa
     7441 tgcgcacggc gaaccgtggc gcatcggtat agagcaaccc aatatcatcc aaggcggcaa
     7501 tacgcagatt atcgtcccgc tgaacaaccg ttcgcttgcc acttccggcg attaccgtat
     7561 tttccacgtc gataaaaacg gcaaacgcct ctcccacatc atcaatccca acaacaaacg
     7621 acccatcagc cacaacctcg cctccatcag cgtggtctca gacagtgcaa tgacggcgga
     7681 cggtttatcc acaggattat ttgttttagg cgaaaccgaa gccttaaggc tggcagaaca
     7741 agaaaaactc gctgttttcc taattgtccg ggataaggac ggctaccgca ccgccatgtc
     7801 ttccgaattt gccaagctgc tccgctaaac atacaaaccg attaggaaac accatgaaaa
     7861 ccctgctcct caccttcggc atcttcctga ccgtcatcat cggtatggcg gtcggctata
     7921 ttttctccaa acgcaccatc aaaggcagtt gcggcggcat taccgcttta ggtatgaaaa
     7981 aaatgtgcga ctgcgacaca ccttgcgaca ccctgcaaaa aaagctggat gaagaaaaac
     8041 aggcaggcgg catacgggtt gaccgttaac gccgaaacaa aatgccgcct gaagcccggc
     8101 cggcgggttc agacggcatt gccgctttac ttcgatcggt ttatacgcaa aacgcgcttc
     8161 aggaaataaa tcagattatt ccccagacgc cttcaggctt ccttccgtgc caccgtaacc
     8221 atagccgggc gcaacacacg gtcggacagg gtataaccct tcttcatcac accaaccacg
     8281 gtattgggtt cctgctcact ggccaccgcc tgcatcgcct gatggatatt cggatcgagc
     8341 ttatcgcccg ctttaggatt gatttccttg atttgcgtgg catcaaacgc cttctgcaac
     8401 tcattcaaag tcatctgcac gcccattttc agcgcatcga aattaccgct ctgatccaaa
     8461 agcgccattt ccagataatc cttgaccggc aacatttcca cggcaaactt ctgtccggcg
     8521 aacttgtgcg tatcggcaat ttcctgctgg tggcggcggc gcaggttttg ctcgtttgcc
     8581 aaagcgcgca gttgttcgtc tttcaactgc gcttccaact cggcaatccg cgcctgcaaa
     8641 tcctcataag ccggctcggc ggcagcctgt tcctgtacac cgtccgcatt tcctactgtc
     8701 tcgacggttt ccaccgcctc cacattttca accgcttctt cactgttttg ctgctgtgtc
     8761 tgttcgctca tatcgtatcc ctcaaaacaa attggaaatc aaaatccggt tattacccga
     8821 gcaagataag gacattttca agaaacttca agcaaaggca ggaaatttca catccgccgc
     8881 cgaataccct accgcaaaaa ccatataaac ggtaagatac cgccgacctt atgcttttca
     8941 gacggcatcc attatgaaaa aaaaccattt gaacacaacc ggtttcgacc tctggcacac
     9001 catccgcgaa gaaaccgcgg ccgctgccgc cgccgaaccg atgctggcaa gttttttgca
     9061 ccaaaccgtg ttgcgccacg agtccctcgg ctccgtcctt gcctaccacc tttccagcaa
     9121 actcggcagc ccgattatgg acgtgcgcgc gctgtttgaa atttaccagc aggcattggg
     9181 cagcgacacc caaatcagca aatgtgtcga agcagactta aaagccatct acgaacgcga
     9241 tcccgcctgc gacgaatatt cgctgccgct tttatatttc aaaggcttcc acgcgattca
     9301 ggcacaccgc atcaaccacc ggctgtatct cgacggacgc aaaacgctgg cgtatttcct
     9361 acaaaaccgt atgtccgaag tattcggcgt ggacatccat cccgccgccc gtttaggata
     9421 cgggctgatg ctcgaccacg ccaccggctt tgttgccgga gaaaccgccg tgttgggcaa
     9481 caatatttcg attttgcacg gcgtaacgct cggcggttcg ggcaaagaag gcggcgaccg
     9541 ccaccccaaa atcggcgacg gcgtgatgat cggcgcaaac gcctcgatat tgggcaatat
     9601 ccgcatcggc agcaatgcca aaatcggcgc gggcagcgtc gtggtttcag acgtgccgcc
     9661 gtccatcacg gttgtcggcg tacccgccaa acccgtggcg cgatcgctca aaaccccgtc
     9721 ggcggatatg gatcaaaata tccagtttgc cgaaatcgac tttatgattt gaatatccgc
     9781 accaatgccg tctgaaacgc caaacgggct tcagacggca ttgcccgctt caagcctgcg
     9841 ccttaatatg ccgccgatgc cggcacttat cagtcggaca aaatgccttt tattcgatac
     9901 gcccgattcc cacgcaaccg gaaataaccg gtttccttgt aaacgggcgg agtcgggcga
     9961 tgattcggac aaagcctatt tcaaaagcaa aatcagcagc aaaacaaccg ctatcgctgc
    10021 cagccacagg ctttgccgct gctgcacttt gaccaaatgg atataggcat cgcgcatttc
    10081 ctgccggcgc gcctcatcga ccaaggcatt gattttgcgc ggcagggaag ggatgatttg
    10141 cgcccagtcg ggggcttcgt ttttgaggtt gcgccaaagg gctttgggac cgacctgccc
    10201 gttcatccat ttcaccaaaa acggtttggc ggttttccac aagtccaaat cgggatcgag
    10261 ttggcggccc aagccttcga tgttgagcag cgttttttgc agcaatacca gctgcggctg
    10321 gatttcgaca ttgaagcggc ggctgacttc aaacaggcgc atcagtacca agccgaagga
    10381 aatctgcgaa atcggtttgt tgaacacagg ctcgcacacg gcgcgaaccg ccgcttccaa
    10441 ctcttccgca cgcgtgtcgg caggcaccca gcccgattcg acgtgggcgg tggcgacacg
    10501 tcggtaatcg cggttgaaaa aggcgaggaa attgatggcg agatagcgtt tgtcgtaatc
    10561 ggtcagcgta ccgacgatgc cgaaatcaag ggcgatatag cggttgtcgg cggcaaccaa
    10621 aatattgccg gggtgcatat ccgcgtggaa aaagccgtcg cggaaaactt gcgtgaaaaa
    10681 gatttccacg ccgtaatcgg caagtttgtg caaatcgatg ccgtctgctt tgagtttggc
    10741 gatgtcggaa accggcgtgc cgtccatcca ttcaatcgtc agcacgtcgc ttgtgcagta
    10801 gtcgtaaaac accttgggca caatcagcat attgctgttt tggaaattgc gcccgagttg
    10861 tcctgcattg gcggcttcgc gcatcaaatc caattcgtcg tgcaggtatt tgtcgaactc
    10921 cgccaccact tcgcgcggct tcagacgctt gccgtctgaa aacaagcgtt cgacccaagc
    10981 cgcgccgaaa cgcatcagcg acaagtcttg ttcgataagg ggcaagaggt tggggcgcaa
    11041 aactttgacc gccactcgtt cgcccgaatg caggcgggct ttgtgtactt gggcgatgga
    11101 cgcgctggcg acgggctcgg tttcaaattc cgcatacagc ttttcgatgg gctgacccag
    11161 cgatttttcg atttgttcac gcgaaagccg cgcgtcaaaa ggcggcactt tgtcttgcag
    11221 ctttgccagt tcgacggcgt aatcgtgcgg aatcaaatcg gggcgtgtgg acaaaacctg
    11281 cccgaacttg atgaaaatcg gccccaggct ttccaaggca aggcgcagac ggacggcagg
    11341 cggttcgttt ttcaatttgg acgactgcgg catcattttc agcagagcgc atatccaacc
    11401 gctacccatc agcgaaacgc acagacctgc cagccgatag cggtaaaaag tcccgacaat
    11461 gaccgtcagg cgtttcaacc atttcatata taaaatcccg aatacagcat ttatcggaca
    11521 atcaaaaaat cttaccgagc agatagccca aaaaggcaat cagcaacccg aaaaccagcg
    11581 gtttgatatt gcggggcggc tcgggttgcg aagatgcgga aatatcatct tcccgcccgc
    11641 cgtgaagttc aagatattcg ggcatttccc catccgcgcc tcctccttcg ccctccccct
    11701 cctccggcgg cacgaccgtt tccaactcca tatacgggta ataatatcca aacccaaata
    11761 gctgaaataa tcatgcaact cttcccgttt cgcccaagtg catttttcct caaccagcac
    11821 ttgcggtttg gaatcgagcg attcgatcaa tccttccgcc ttttcttcgc cgagattctc
    11881 cttaaggcac tcccggataa gcccgcgatc cacttggggc gggtaggaaa catatacatc
    11941 gtacaaatcg tttcggtaat ctttactcat ctcaatcata agggaacaaa tacgcctgat
    12001 accgttccgc ccgttccttt cgggaaaccc gattcatctt caagcaatcg gattatatgt
    12061 aaattcatcc cgataatata ttttccaaaa gtaaaaatgc cgtccgaacg ttcagacggc
    12121 ataacacaaa ccagccggtc acacggaaaa cgacgatccg caaccgcagg ttgtttccgc
    12181 attcggattg cggatgacga actgcgaacc ctgcagactt tccgtatagt cgatttccgc
    12241 accgaccaga tattgatagc tcatcggatc gaccaaaaag accaaaccgt ttttctcaat
    12301 ttcaaaatcg tcgtcgtttt tgatttcgtc aaaagtaaat ccgtactgga aacccgaaca
    12361 gccgccgccg ttgacaaaaa cccgcaattt caaatcggga ttgttttctt cggcaatcaa
    12421 atcggcaact ttggcacagc agctgtcagt aaaaataata gggctttcgt ccgacatggt
    12481 gttattcctt aatataatat tttctgcatt ctctctcaac cccccttcaa aggcaagcag
    12541 ggcttcgcct ttgaaaaagg aattgtccca atagataagt ttgaccgcct gcaatttcaa
    12601 gccccaaact caaaccgtcc tgccgaacca ctcgacacgc ccgataatct ccacatcatc
    12661 ggtcaagtcg ttcaaattga tttcaaaagc aggataagcc tcgttggcgg aaatgacatt
    12721 gataatcccg ccggggacga tttgcaggcg tttgaccaac agattttcat taatccgcaa
    12781 cacatacagt ccgtctctcg gcgtattttc cccatgattg accaaaatcg aatctccgtc
    12841 attcaaaacc ccctccatcg aatccccctt gacggaaatt acagacaggt ttttcgtatc
    12901 tcgggtaaca taattctcaa tccaatgccg tctgaacgcc attgtaaata ccggttcctc
    12961 atgaccgaca aactgcccgt atcccgcagc cgcccgaata tcatatctcg gcacaaagac
    13021 aaactcgtcc gtatcaactt catttcccaa ggtatcgtaa gcaagggatt tttttggggc
    13081 ttcatccgga aacggattac cctcccctgt cagcagccaa tcgatactac accccttcaa
    13141 ctgcttaatt tttttcaatg tttcagactt cggcagaccg ccttcattcc atattctgct
    13201 gaagcccgca atcgtcattt caatatcgga tgcgattttt gcctgcctcg cttcactttt
    13261 ccataaaaaa accagtctgt ctttaaaggt ttccataaat atccttccgc tccccttcat
    13321 tcaatcatct aaagtaaaca tgaaatatat tttacattag ctatcccaaa aacggaataa
    13381 ctatgataaa ctaaatttaa attatgattt ttcttatcca atacataatt tttcagattt
    13441 tccataggtt taatcccgaa ttgacagaaa aaggatagaa aaatgaagaa aaccagcaaa
    13501 tatcttatct atactgcggc atttacctca ttctgctttg ccttccaaga aaaccgttct
    13561 gaagccaaac agcccgacat cactttatcc gcatccctgt gcgaacaatt caacatgctg
    13621 aacgccaaag atatggatac agaacaagtc tccctttcca aagaatgcga catcatcgag
    13681 tcttcacacg actgggaaaa agagtacggc aacttgaacg aacaggaaat gctcgccggc
    13741 gtcgtctatg aataaacctg cctgccattt gaaacattat gcttgaatgc attggagcca
    13801 aatgtattaa atcaaatata aaaccaatat attcataaag ttatatactt atagccatgc
    13861 tgtagcttga aacagcccgc cgcccgccct atttacagcc catcgggaca aaatgttcca
    13921 acatatttct caaaataagc aaaatcaaat aggagtatcc acatggcaaa agtaatcggt
    13981 atcgacttag gtacaaccaa ctcttgtttg gccatttccg aaaacggtca aaccaaagtc
    14041 atcgaaaacg cagaaggcgc acgcaccacg ccgtccatta tcgcttattt ggacggcggc
    14101 gaaatcctcg tcggcgcgcc tgccaaacgc caagcggtaa ccaacgccaa aaacactatt
    14161 tacgccgcca aacgtttgat cggtcacaaa tttgaagaca aagaagtcca acgcgacatc
    14221 gaatctatgc ctttcgaaat catcaaagcc gacaacggcg acgcatgggt aaaagcacaa
    14281 ggcaaagagc tgtctcctcc tcaaatttcc gcagaagtcc tgcgtaaaat gaaagaagcc
    14341 gccgaagctt acttgggcga aaaagtaacc gaagccgtga ttaccgtccc tgcctacttc
    14401 aacgacagcc aacgtcaagc caccaaagac gcaggccgta tcgccggttt ggacgtaaaa
    14461 cgcatcatca acgagccgac cgcagccgct ttggcattcg gtatggacaa aggcgacaac
    14521 aaagaccgca aaatagccgt atatgacttg ggcggcggta ccttcgatat ttccatcatc
    14581 gaaatcgcca acctcgacgg cgacaaacaa tttgaagtat tggctaccaa cggcgatact
    14641 ttcttgggcg gtgaagactt cgaccaacgc ttgattgact acatcattga cgagtttaaa
    14701 aaagaacaag gcattgattt gaaacaagac gtaatggctc tgcaacgtct gaaagaagct
    14761 gccgaaaaag ccaaaatcga attgtccagc ggccagcaaa ccgaaatcaa cctgccgtac
    14821 attaccatgg acgcaaccgg cccgaaacac ttggcaatga aaattacccg cgccaaattc
    14881 gagagcctgg ttgaagacct gattgcccgc tctatcgagc cttgccgcac cgcattgaaa
    14941 gatgccggct tgagcaccgg cgacatcgac gacgtgattt tggtcggcgg tcagtcccgt
    15001 atgccgaaag tacaagaagc cgttaaagac ttcttcggca aagaaccgcg caaagacgtg
    15061 aaccctgacg aagccgttgc cgtaggtgca gcgattcaag gcgaagtatt gagcggcggc
    15121 cgcagcgacg tattgctgct ggacgtaacc cctctgtcct tgggtatcga aaccatgggt
    15181 ggtgtgatga ccaagctgat tcaaaagaac actactattc cgactaaagc gtctcaagtg
    15241 ttctctactg ccgaagacaa ccaaagcgca gtaaccatcc acgtactgca aggcgaacgc
    15301 gaacgcgctt ctgccaacaa atctttaggt caattcaact tgggcgacat cgcacccgca
    15361 ccacgcggta tgccgcaaat tgaagttact ttcgacatcg acgccaatgg tatcttgcac
    15421 gtttccgcca aagacaaagg cactggcaaa gcggccaaca ttaccatcca aggttcttca
    15481 ggtttgagcg aagaagaaat cgaacgcatg gtgaaagatg ccgaagccaa tgccgaggaa
    15541 gataaaaagc tgactgaatt ggtcgcttcc cgcaaccaag ccgaagccct gattcactcc
    15601 gtgaaaaaat ctttggcgga ctacggcgac aaactcgacg ctgccgagaa agaaaaaatc
    15661 gaagccgcgc tgaaagaagc cgaagaagcc gtgaaaggcg acgacaaaac cgccatcgat
    15721 gccaaagccg aagcactggg cacagccagc caaaaactgg gcgaaatggt ttacgcgcaa
    15781 gcgcaagccg aagcccaagc cggcgagggc gcacaagcca atgcttctgc aaagaaagac
    15841 gatgatgtcg tagatgccga ctttgaagaa gtaaaagacg acaaaaaata attgatgccg
    15901 tctgaaaaaa acgcgaacca ttcggttcgc gtttttttca attgagataa aagacaatag
    15961 catgacagag attccaaatt catctaccgt gatattccca agcccaagtt ccaactgttg
    16021 catcggttat ctggaaattt ttcatatcat tttcttattt acttaaattt tttaataaga
    16081 taataaataa taattattat catgagctaa gaaatgaaac taaatactct cacatgggct
    16141 ttgatgaccg ttttttccgt tgcgccatct tgggcagaac aaccggcaaa tactgaagaa
    16201 atacaacccg tcaaaacctt ctccccgccc aaaccgattg caccgaccgc cgcacaaggc
    16261 tatttccccg aaaaccaatt cgaccgctcc gaccgcagcg attattactt tgttaccgaa
    16321 aacatagacc aagccttccg tccgctgaag gcaaacagca gtttttacgg caaaagcttt
    16381 tacaactccg ttaccgcgca agcactcggg gcgaaggtat acggcgtagc caacctcaat
    16441 cgcaccaagg caaacggcta caaagatggc ggtgggcgcg acaccgattg gaaatacagc
    16501 cgcttcaatc aggctttggt actcggtttc gtgccgtctg aaaatcaaga ataccgcctc
    16561 acttatctgc acgacgacat caacaacgac cgccagccgc aggtcgtcaa cgacgcattg
    16621 gacaccgaac gccacatctc caaactcaac gtgcgttggg gcaatgccga tttgagcaat
    16681 acggtcagcg cggaagcagg cgtcatcaaa ctcaaacgcc atgccgacaa ttactccttg
    16741 cgaccaaaca acacgccgca gcaagtgttc gtagaacttg accgcaaagt gtatgatttc
    16801 tcgctcaaac acgatgcgga tttcggcaaa ttccacaaca cggcagccgt cagctaccgc
    16861 aacgacagcc aaaacggcga acgcaacacc cacactgcca tgtgcgattt cctcaacggc
    16921 taccgtttcg ccgatgtgca catcgaccgc tggtgcattg ccgatacctt atcttacaag
    16981 tttgacgacc gacacaaact gggcttgggc ttgggcttaa gctacgaact caacgaagcg
    17041 gacattcgca aaaatacggc acagcctgaa aacccaataa aacccggttt ccccttcgcc
    17101 tcatcacaac aaatctggaa aacccattac ggctacgact tcaacggcaa agtgcgccgt
    17161 catgctttgt cgggcgaact caaatacgat ttcacgccgt ctgaaacgca aaaatacagc
    17221 gtttccctcg cgcatttgga acgcatcggc gacaacaccg aacgcttcaa ctcgcttgct
    17281 gccatcgtgc aaaaccgtat gagcggcatg ctgatgaacc aaaatccagc cgccgccatt
    17341 gcaggcaatc cccctgctga aaactgaaaa acacaaccgc atcaggctca ccgccgacag
    17401 ccgcaacgac tactacaacg gctacatgaa ctcgctcgca ggcgcgggct ggaacgtggg
    17461 cggcacgctt gtggcggaca aagtcaaaga cctgattatt tttgaccgcg cacacggaca
    17521 aagcggcaca gcttccaaag acggcggcat catcacacgc aacgtggacg cacgattgtt
    17581 taccgcgcag gcctacgcgc gttacaactt caatccacat tgggcggcag gcatcaaagc
    17641 cgcctacaac tacggacaca acgaaaccga cggcaggccg ccctatcaaa tccgtccgtt
    17701 tgaagccgcc gtccaagccg actacaaaaa ctactttgcc cacggcagct acaacatcgg
    17761 cgcggcaaca cgctttgtcg ccaaacaaac gcgcggtgat tttgatatgg caagcggtct
    17821 gggcatagac aaacgcgaag ccgccaaagg ctttaccgtt gccgacgtgt acgcaggcgt
    17881 aaacatcaaa gacaaatacg gcttgcgctt gggcgtgaac aacgtattca acaaaaaata
    17941 cgtcgaatac atcagcggcg accacgtcct cgccctatcc cccagcgtag tgtatgcacc
    18001 gggcaggaca tattggttga gtttgcatgc ggcattttaa aaccatgccg tctgaaaaat
    18061 acctgcattg gcttattaag aattaggaga taaaaaatgg atatgaaaag acgcgatttc
    18121 ttaaaaatga ccgccgcgct ggcagccgca ggcgtttcgc cttctctgct tactgccggt
    18181 aaagagcaat ttaccgtgta cggcgcaccg gcaatgccca gcgttaccat tgccgtaacg
    18241 gcgttgcaag gtaaactggc gaaacaggcg gacgtgtcgc tgaaaatctg gcgttcgccc
    18301 gaccaactgc gcgcgggcgt ggcaagcggg caatttaaag tgatgatgag tccgagcaat
    18361 gtcggcgtaa acctgcgcaa ccaagggcag aaagtcggca tggtgaatat tttgaccaac
    18421 ggcatcacgc agttggtctg caaaggcagc gcgattgcct cgccgcagga tttggtcggc
    18481 aaaaaaatcc tcgtgccgtt taaaaacgat atgcccgaca tcgtgctgca agccttgttg
    18541 aaaaaactga aaatcgacgc acacaaagtc agcattacct acgctgccac accgcccgaa
    18601 gcagtcggac tatttccaag taaaggttac catgccgtca tcctgcccga accgatggca
    18661 accgccagcc tactgaaagg caaaaccata ggtataaacg tcgtgcacgg ttttgatttg
    18721 gtaaaagcat gggggcaggc gtttggcacc aaaccgctga ttccgatggc aggtatcatc
    18781 gccaacgaag aatatttcca cgcacacaag gcgcagttcg acatcttcca tcaagatttg
    18841 aaaaacgcgc tcaactggat attggccaac cgccaaaacg cggcgaaaat cggcaaaaac
    18901 tacctacccg cccccgaacc cgccctagtc atgggcttgg acggcgcacg gctgacggta
    18961 agcaaaggca gcgaagtgaa aaacgagatt ttgaagtttt acgaaatcct gatgcagttc
    19021 aacccggaac ttttgggcgg caagctgccg gataacgggt tcttcttggc ttaaagggaa
    19081 agaggccgtc tgaaaagggt agggtggttg tgaatacctg atttatcatt tttcagacgg
    19141 cctattaagg cattgtcagc ggcgtgggtc ggattcttga atccgacatt tcggctaaac
    19201 ggggctgtcg gatacaagta tccgacctac tcttttcaga cggcctatca gataatagtt
    19261 cgaacagtac acaaagagcc gtcggattgc ctgaaatgtt gggtcatgac ccaacctacg
    19321 cttactgggg aaacgggatc gaagtgtgag gtatgtggcg gggttgttta ataatgaaat
    19381 tttataacta tttgataggg tggtaatgcc atgaatgaag ataaatatag ggaaaatatg
    19441 acatgggatg aaattgaaaa tgtagtagga actttagttt ctgaagaaag aatgcttaaa
    19501 tccaatagct ttttaagtat taaaaatagt aagaataggt tggcttaatc acaaccataa
    19561 cccaacgaaa atactaaaat tccaagctaa ggctatcctt tccaaaccat ccccatcaac
    19621 caaaccatga ttaaaaccga caaaatccgc aaaccgcagc ctgcgctgtt ttacatcatc
    19681 gactaccttt ggagcgactt tgccggtctg agcgtggcga tggtggtggt ggcgttgtgg
    19741 gcgtggggca gcgccgtgtt cggcgagttt atgctgcctg cgccggtaga ggtgtttcaa
    19801 aaatctttgg atttattgaa acattttcag gaaaatgaaa tcgggatttc gctgtggcgg
    19861 tcggtagtgg gtatttcggt tgctttgata gcaggattgg cggcggggct ggtggcgggc
    19921 agttttaaga cggcgatggc gttgcttaag cctgtgatta cgattttgtt agcaatgccg
    19981 ccgattattt gggtggtgat ggcgttgttt tggttcgggt tcggcaatcc gagcgtgttg
    20041 tttaccatca ttgtgttggt tgcgccgctg acgtttgcga gtgcggcggt cggaatggcg
    20101 agcgtgaaca agcagcatga ggagttgttt gacgcttata aattaggccg tctgaaaaaa
    20161 atccgctatc tgtatatccc gcatctgacg ggctatgtga tttccagcgt cggcgtggcg
    20221 gtggcgatgg gggtgaaggc ggtgatcatg gcggaactct taggcgcgag caaaggcgtg
    20281 ggcgcgcgga ttgcggacgc gagggcaatg ctgtagactt cgacggtaat ggcttatgtg
    20341 gtgttggtca tggtatttgt gtcgctgttt gaatacctga ttccaagcct ttgtaaattt
    20401 tgtttatgcc gtggaggaga tgatgctctg tcttgaaaac gtgcgttttg aaattctccg
    20461 cgaccccatc gtgcgcgatt tcagtttgaa cctgcaacag ggcgaagtga aagccttgtt
    20521 cgggccgagc ggctgtggca agacgacggt tttacggctg attgcgggct tggaaacgcc
    20581 gaaatcgggc acgatacgta atactttcca caaaacgggt tttctgtttc aggaaaaccg
    20641 cctgccggaa aacctgaccg cgatgcagaa tatcgcgatt tttatggaca acccagacga
    20701 aggcgaaatc gtcgcgctgg cggcgaaagt cgggctgact gcgggcgatt tgaacaaata
    20761 tccgaccgaa ttgtccggcg gcatggcgaa acgggtggcg tttttgcgcc tgctgctgtg
    20821 cggctgcgac ctagccttgc tggacgaacc gtttgtcggt ttggaccgcg atttgcgcga
    20881 tattttggtt gctatgctgg tggaaaaaat cgagcggcag ggcatggcgt gtatactggt
    20941 aacgcatgac cgcttcgaag ccgcgcgcct gagccatgaa attatgctgc tttccgctaa
    21001 gggcatgaac gtgcaaaacg tgataaccct gcccacgccg ctgtccgaac gcgattcggc
    21061 ttttgaagaa gtcgtggtgg caagggagtt tcaggggatt cattattatg agtgatgagg
    21121 ggattttgag tgtctgacaa accttacggt tcgttgccct ctccctaacc ctctcccacg
    21181 gggagagggt tagcaagcct caggcttgcc tctttagcga aagatacgaa tctgttatcc
    21241 gaacgcgatt cggtttttga aaaagccgtg gtgccaaggg ggcaggggat tcattatgag
    21301 gtgctttatg ttttcgactg tgattactgc tgctgtttta tatattgcta cagcagtaga
    21361 tttgttggta atactattaa tattttttgc tagagcaaat actagaaaag aatatcgaga
    21421 tatttatatc ggacaatatt taggttctgt aattttaata ttagttagtt tatttctagc
    21481 ttttgttttg aattatgttc cggaaaaatg ggtgttgggt ttattaggtt taataccgat
    21541 ttacttaggt attaaagttg ctatttacga cgattgtgag ggcgaaaaaa gagctaaaaa
    21601 agaattggat gaaaaagggt tgtcaaaatt agtcggtatt gttgctttgg ttacagttgc
    21661 tagttgtggt gcagataata ttggactttt tgttccttac tttgtgactt tagatcttgt
    21721 cgacttatta gttactcttc ttgtattttt aatattgatt tttgttttag tatatacagc
    21781 acaaagattg gctaatattt caggtgttgg tgaaattgta gagaagttta gtcgttggat
    21841 aatggctgtt atttatattg gtttagggtt atttattatt attgaaaata atacaattcg
    21901 aacaataata tcaataatat gaatgacaag ggcatttcag tatctgacaa accttgcggt
    21961 tcgtttccct ctccctaacc ctctcccaca ggagaggggt tcaggttgct gaaaatcaga
    22021 gggcgaagga ttggggatca aatggaaggt aaaacctgaa aatgttcggg tttgacagcc
    22081 cgattccctc tccccgtggg agagggctag ggagagggca agcaagccgc aggcttgcct
    22141 ctttggcgaa aaatacaacc ctgtccgccc aataaatcca tacccgaaag catcttcata
    22201 cagaatcaag ccaagcgatg aataaatttt ttacccaccc tatgcgcccg tttttcgtcg
    22261 gtgcggcagt acttgccata ctcggtgcgt tggtgttttt tcatcaaccc cggcgctatc
    22321 atcctgcacc gccaaatttt cttggaactt atgctgccgg ctgcatacgg cggttttttg
    22381 actaccgctt tgttggaccg gacgggtttt tcaggcaacc tgaaacctgc cgctactttg
    22441 atggcggtgt tgttgcttgt tgcggctgtt ttattgccgt ttttaccgca acttgccgca
    22501 tttttcgtcg ccgcctattg gctggtgttg ctgctgttct gcgcctggct gatttggctc
    22561 gaccgcaaca ccgacaactt cgctctgttg atgttacttg ccgcatttac cgtttttcag
    22621 acggcctatg ccgtcagcgg cgatttgaac ttactgcgcg cgcaagtgca tttgaatatg
    22681 gcggcggtca tgttcgtatc cgtccgcgtc agcgtccttt tgggcacgga aaccctgaaa
    22741 gaatgccgtc tgaaagaccc cgtattcatc cccaacgtta tctataaaaa catcgccatc
    22801 accctgctgc tgcacgccgc cgccgaactt tggctgcccg cgcaaaccgc cggttttact
    22861 gcgcttgccg tcggcttcat cctgctcgcc aagctgcgcg aactgcacca tcacgaactc
    22921 ttacgcaaac actacgtccg cacttattac ctgctccagc tctttgccgc cgcaggttat
    22981 ctgtggacag gcgcggcgaa actgcaaaac ctgcccgcct ccgcgcccct gcacctgatt
    23041 accctcggcg gcatgacggg tggcgtgatg atggtgtggc tgactgccgg actgtggcac
    23101 agcggcttta ccaaactcga ctacccgaaa ctctgccgca tcgccgtctc catccttttc
    23161 gcctccgccg tttcgcgcgc tgttttaatg aacgtgaatc cgatattctt catcaccgtt
    23221 cccgagattc tgaccgccgc cgtgttcatg ctttacctgc tgacgttcgt accgattttt
    23281 cgagcgaacg cgtttacaga cgatccggaa taaaaatgcc gtctgaaacg gttttcagac
    23341 ggcatcggat tgccaatgat caagcctcct gcggatcgac atccaccgac catcggattt
    23401 tgccgtcgcg gttctgctgc aacacctgca cccacaaact cacggcgcgg tgcaaatctt
    23461 gtcgggatgt tgattcgagg aaaacttgcg cacgttcgcg ttcggcaagg cgcaccatca
    23521 gcatcggagc ggcaccgaac cgggagacgc tttcgggcaa gagcggggcg agggtttctt
    23581 tggcggcgtt gagaaattcc atcgcatcgg caacacgcgg cgcatcggca cggacggccg
    23641 tctgaaaacc gaaaggcggc atggcgaaca tttgccgttc gttcaattcg ttttcggcaa
    23701 acacggcgta gtcctgcgct ttgacggcgg cgaagacagg gtgttcgggc agttgggtct
    23761 gtatcagcac cttgccgggt ttgtcggcgc gccccgccct gccggacact tgcatcagct
    23821 cggcaaacag cctttccggc gcacggaaat ccgcgctgta caggctgccg tcggcgttca
    23881 acacgataac gaggttgagc cgcgcgaaat catggccttt ggcaagcatc tgcgtgccga
    23941 ccagaatgtc gatttcgtcg ttggcgatgc ggcggtacaa atctgcccag tcgtttttgt
    24001 gcgccgtact gtccctatcg acgcggacga cggctgcctt gggcagcaag gcgcgcaggg
    24061 tttcttcgac gcgctgcgta ccgtgtccga cggcggtcag gtcttggttg ccgcagtcgg
    24121 gacatttgaa cgggatgggt tcgcggtggt cgcagtggtg gcagcgcagt tggcgggcgc
    24181 gttggtgcag caccattttg gcggagcagt tcgggcagcc gaaggtatgg ccgcagtcgc
    24241 cgcaaaacag cgcaggcgcg aagccgcgcc ggttaaggta caccagcgac ataccgcccg
    24301 cttcaaagtt ctgtttcaaa agctgcaagg cttgcggtga gaagccgtta tcgagtttca
    24361 gacgacctat gttgaggatg tccacttgtg gcagttgcgc ggaagcatgg gcgcgttcgg
    24421 tcagttgcag caggcggtac gcgccgcttt gcgccttgtg ccaactctcc aagctgggcg
    24481 tggcactgcc caacacgacg ggacagccgc cctgcttcgc ccgccacacc gccaaatcgc
    24541 gggcgtggta gcgcaattcg ttgtcttgtt tgaacgagcc gtcgtgttcc tcatcgacca
    24601 caatcagccc gacatcaggc aaaggcgtaa acaccgccag ccgcgtgccg atcaccagct
    24661 tcgcctgccc caacatcgcg cgcagataat cctgcgtgcg cctgcctgcc gccatccggc
    24721 tgtgcaacac ggcggtcggc acgtcggcaa aacggttttc cacccgcttt aaaagctgcg
    24781 gcgtgaggtt gatttcgggc aacagaaaca acacctgtcg cccctgcgcc aacactttgg
    24841 ccatcgcatc gaaatacacc tcggtcttgc cgctgccggt aatgccgtac agcagaaacg
    24901 accggaagcg tcccaatgcc gtctgaattt catcggaagc cttctgctgg ccggtattta
    24961 atacaaattc agagtgcgaa gcctgcccgc ggtacggcct taaaacaggt ttcgccgctt
    25021 ccgtcgtttc aatccagccc tgctccgccc aatcctcaat caatttcgcc gcctgcgcgt
    25081 ttgcctgttt caacgccgcc atcgtcattt cgcccgacag cagcgcgtcc cacaaagccg
    25141 cttttttgtt gaaccgagcc ggcggcggcg tttgcgccct gcccgcttcg ttcaaagcat
    25201 aaaacaacgg cggctgcggc atttccaccg cgcgcgtttc cttcaaaccc tgcggcaatg
    25261 cggcaaatac agcctgccca gtcggatagt ggtaataacg cgacgtaaac gccaacaaat
    25321 cacgccagct ttgagacagc ggcttttctt ccacaaagac cgtctgaacg ctcaaaatcc
    25381 gcgccgcatc catatcgggc gcaatatccg tttcccacac catcccgaca acagttttat
    25441 tgcgaaaagg cacaagcacc cgcgttcccg gaggaagcgg ctcggaatgc gaataagtca
    25501 aaaggccgtc tgaaagcggc acgtttacag cgatgcgatg gtagatcata caggcaggca
    25561 aatcattttt ttgcgtattt taacagccgg aagctatttt cagacgataa attgagcctc
    25621 ggcgcaatgc aggcgcaaca gaggtttgat acaatgccac ttttccaaca cgctcaatca
    25681 tctttgatat gaaaaccaag ttaatcaaaa tcttgacccc ctttaccgtc ctgccgctgc
    25741 tggcttgcgg gcaaacgccc gtttccaatg ccaacgccga atccgccgtc aaagccgaat
    25801 ccgccggcaa atccgttgcc gcttctttga aagcgcgttt ggaaaaaacc tattccgccc
    25861 aagatttgaa agtgttgagc gtcagcgaaa caccggtcaa aggcatttac gaagtcgtcg
    25921 tcagcggcag gcagattatc tacaccgatg ccgaaggcgg ctatatgttc gtcggcgaac
    25981 tcatcaacat cgacacgcgc aaaaacctga ccgaagaacg cgccgccgat ttgaacaaaa
    26041 tcgacttcgc ctccctgcct ttggacaaag ccatcaaaga agtacgcggc aacggcaagc
    26101 tgaaagtcgc cgtcttctcc gaccccgatt gtccgttctg caaacgcttg gaacatgagt
    26161 ttgaaaaaat gaccgacgtg acggtttaca gctttatgat gcccattgcc ggcctgcacc
    26221 cagatgccgc gcgcaaggcg caaatcttat ggtgtcagcc cgaccgtgcc aaagcgtgga
    26281 cggattggat gcgtaaaggc aaattcccgg tcggcggcag catctgcgac aatcccgtcg
    26341 cggaaaccac ttccttgggc gaacagttcg gcttcaacgg cacgccgacc ctcgtcttcc
    26401 ccaacgggcg cacccaaagc ggttacagcc cgatgcccca actggaggaa atcatccgca
    26461 aaaaccagca gtaaacccgc aatgcaaaag gcttggaacc atgttccaag cctttttcaa
    26521 atgccgtctg aagcctgtcg cggcagcttc agacggcatc tttgttcaaa cctcaatcct
    26581 gcgccaatgc atctatcgga ttgagtttgg ctgccttatt ggcaggcata aagccgaacg
    26641 cgatgccgat tccggtcgaa caggcgaccg ccccgataac ggatgccgcc gaaatgtcca
    26701 tcgggaaatc ggttacaaaa tgattgaaca cgaggctgac ggcggcggac aaacctacgc
    26761 cgaccaagcc tccgatgatg cagattaaca ccgcctcaat caaaaactgc tgcaaaatat
    26821 tgccgcgccg cgcgccgatt gccatccgta tgccgatttc tttggtgcgc tcggtaacgg
    26881 acaccagcat aatgttcatc acaccgatgc cgccgactac caatgaaatc agggcgatgg
    26941 aggaaatcag cagcttcatc gtaccggtgg tgctttcgac catctgcctg atgctgtcgc
    27001 tgttgttcat aaagaagtct tccgtgccgt gccgtgcttt gagcagctcg gccagccctt
    27061 tttcggcaac ccgggtattg gcattgtctt tgattttgac ggtgatggag ttggtgtggc
    27121 tctcgcctgt gatttggtgc atcaccgtcg tatagggcga ccaaagcatc agcacgtcgg
    27181 aattgccgaa agcgttttcg tcttttttca tcacgccgat gacggtcaag gggcgtttcc
    27241 tgaacaaaat ggttttaccc aacggatccg agtccgcaaa gagtttgtct ttgacatttt
    27301 ggtcgatgac gacgacttgc gcgtcttctt tcacatcgtt ctcatcaaac agccgccccg
    27361 tttccagctt cagcccgcgc acgtcgaaat attgttcgcc cacgccgtac aaagaagcgg
    27421 tcaggtcggt attgcggtag gtcagcgtgc cgccgctcga agtcatgggc gtggcggagg
    27481 caacgtagct ttgtttggcg atgatttttg cgtcgtctat ggtcagggtt ttgattttgc
    27541 cgctgcgcct gtcgccgaag ccgcgcccgg ggaagatgct gatggtgttc gtccccatcg
    27601 aactgatgtc ttcgaggatt tttttctgcg aaccgttgcc caatgcgacg acggagacaa
    27661 ccgaagcgat accgatgatg atgccgagca tggtcagaag cgaacgcatt ttgtgcgcca
    27721 atactgcttg taccgacatt ctgaaggctt cgacaaactg gtcgtaataa aacgaccacg
    27781 aggctttttc ctgaatcctc ccgacattgc ttgcggggat ttcgggattt ttcgaggtgt
    27841 cggaaatgat ttcgccgtcc cggatttcga tgacgcggtt ggcgttggcg gcaatgccgg
    27901 ggtcgtgcgt gaccataatg acggtatgcc cggcttcgtg cagcctgcgg atgatttcca
    27961 tcacattttt gccgctggcg gtatcgagtg cgccggtcgg ctcgtcggcg aagatgattt
    28021 ctccgccgtt catcagggcg cgggcgatgg agacgcgctg ctgctgtccg cccgagagtt
    28081 cgccgggctt gttgccctct ttacttgcca aacccaaatc ctgcaagagt ttgtccgccc
    28141 gcgcggaacg ctctttgccg cccataccca tatacacggc tggcagcgcg acgttgtccc
    28201 ttgcggtcag cgagcttaag aggttgtagc gttggaagat gaaaccgaag cgttcgcggc
    28261 gcaatgccgc cagttcgtca ggctgcattt tggcagtttc gatgccgtcg attcggtacg
    28321 aaccggaacc ggcggtatcc aaacaaccga gtatgttcat cagcgtggac ttgcccgaac
    28381 cggactgccc gatgatggcg acaaaatcac ccttctctat cgacagactg atgtctttca
    28441 aaatatggac gcggttctcg ccgctgccga agcagcggtt gatgttttta cattcgatca
    28501 agctcataat attccgtaaa tcaataacaa atgccgtctg aaaccgtttc cgtgttcaga
    28561 cggcatattc gtttatcggc gcggcgggcc gcccatgacg cgttcgccgc tttcctgttg
    28621 ctcggcggcg gttatttcgg agatgaccac tttgtccccc tctttcaaac cgcttttcac
    28681 ttcggtattc atactgtctt tcataccggt tcggatttcg cgttccactg ccttgccgtc
    28741 cgcacccaac acgcgtacga acgccttgcc gccgcgattt ttcacggtca gcgacggaat
    28801 aagcaacaca tttttcacac cgtcgatttc aaccgtattc tgcgtcgtca tccccgtggc
    28861 gagtttgccg tccggattcg gcacaaacga acgggcataa taatagaccg cattggaagc
    28921 cgtatccgta ctgctgttgt agccgcccga cgacatcgtg gtcagcccgg ggtcgacgct
    28981 gtcgagcttc gccttaatcg gcgtatccgg ttcggacaaa atcgtaaacg aaatatcctg
    29041 ccccgccttc accttggtaa tatcgccctc ggcaatctgc attttgttca acatcatatc
    29101 cagattcgcc aattggacaa tcgtcggcgt agactgcgcc gcgttcacag tctgcccctc
    29161 ttccacggga atcgccacca ccgtgccgtc catcgtcgcg gtaatgcgcg tgtagcccaa
    29221 atccgactcg gcggtattga tggaaatttt gctctgtctg attaaagcct tcaactcggc
    29281 aacattggct ttggcggcgg caagcgcatc ctgcgcgctt tccaaatctt ctttagaggt
    29341 cgcatcatcc ttccacaacg ccgcctgacg cttatatttc ttctccgcgc tgcccaatgc
    29401 aatctgtgcc gacaccagct tcgcctgata cgtttccaat ttggattttt ccatatcgag
    29461 cgtgttggtc tgcgtggtcg aattgatttc cgcaatcaaa tctccctttt tgacctgttg
    29521 cccgagtttg acataaagtt tcttaatctg ccccgaagcc tgcgcgccga ccgataccag
    29581 attcgacggc gaaatctcgc ccgtcgcgga aaccgtccgg ctgatgccgc cgcgcctgac
    29641 cgtttccgta atataagcag cctgcggttc gggcttcaga taatgccatc cgccccaaac
    29701 cgctgccgcc gcgactgccg caacagccga ccatttcatc attttcgcca tattttcaat
    29761 tctctgtatg cgtcaatccc ataaaccgga agcggattct accgcaagtc cctgccccat
    29821 ccaaggcggc aaaccgccga caataaaaat aaccatttgt tttaaatggc ttatacaatc
    29881 tgctttcccg aaaatttcaa tccgtttaca tattccgcca tttccgatac ggcgttataa
    29941 tgccgcccac aaacaaccaa atggaaacac attgctatga aaaacgttca aaaaggcttt
    30001 acgctgctcg agctgatgat tgccgtcgcc atcctcggca tcctgacgct catcgcctac
    30061 ccttcctata aaacctacat ccggcgcgcc cgcctgtcgg aggtcaaatc caccctgctg
    30121 gtgaacgcgc aaaacttgga gcgttactac cgccaaaaag ggacgtttca aacctacgac
    30181 aaaaacaaac tgaaacaaaa cgaatatttc aagattacct taagcgaagt cagccccgac
    30241 cacttcaccc ttcaggccgt tcccaatacg gcgaccaacg aaggcgaaac ctgcgtcgtt
    30301 acgctcaacg acggcggtac cattgccgct tccggtacaa accaatcctg ccccggcttc
    30361 gactgattgg ctgttgcagt cggcattgtg cctaaaccat ctataaaaat gccgtctgaa
    30421 cgctgttcag acggcatttt tgacatcccg atacgccttt caaacggctt ccgccatttc
    30481 caacgcctgc ttgatgtcca cggcgacaac gcgcgacacg cctttttcct gcatggttac
    30541 gccgaccagc tgctccgcca tttccatggt caggcggttg tgggagatgt agaggaactg
    30601 ggtttgcacc gacatttctt tgaccagctt gcagaaacgc gaggtgttgg catcgtccag
    30661 cggggcatcg acttcgtcca gaaggcagaa gggggcgggg ttgaggctga acagggcgaa
    30721 cacgaggctc atggcggtga gggctttttc gccgccggag aggaggtgga tggtgctgtt
    30781 tttcttgccg ggcggacgcg ccatgatgga cacgccggcg gtcaggaggt cgtcgcctat
    30841 cattttgagg gtggcttcgc cgccgccgaa cagagtcggg aagaaggttt ggactttgcc
    30901 gttgacggcg tcgaaagttt ctttgaaacg cgctttggtt ttgtcgtcga tttgggcgat
    30961 ggcttcttcc aaaagcgcga tggcggcctg cacgtcttcg ctctggctgc ggtagtagcc
    31021 gtcgcgttcg cgcgcttctt cgagttcttg cagggcggcg aggttgacgg cgccgagggc
    31081 ttcgatttgc cgggtaaggc tgccgatgct gctgttcaat actttcgccg attctttcgc
    31141 caacgcttcg agcgcgtcca gatcggcagc gcgttcggtc aggttttgat ggtagcgttt
    31201 ggcgttgatc agggcctcct gctgctgcaa cagggcggtt tgggtggcgg cctgaagctg
    31261 cggcagcttg gtttgcaggg tttgtacgcg ggcgtattgc tccctgccct gctcctgaat
    31321 ctgcgcgagt ttttcctgca cggcgatgta ttcttcgtcc agcgtgtgta cggtttcggt
    31381 caactcgtcg agcttgatgt gctgctcgtc gttttggaac tcggtttcat aggcgagggc
    31441 aagctcttgc cggcgttcct gccagtccag ggtttgctgt tcgagccggg cgatttgctg
    31501 ccggtagttt tgtttttgct ggttgagttt gtggacggcg acttcggcaa gcccgtattg
    31561 gcggttggct tccaacaggg caagctgcgc ctgtttcaga cggccttgct gctcttggcg
    31621 gctgtgcgcg gtggtttgct gctggtgttc gagttcggcg gcggcttcct gcaaagtagc
    31681 gatgtcgtct gaaagcccgt cggacgtgtg ttgcaacacg gtctgctctt ccgccaactg
    31741 tgccagttcg cgctcgatgt gttcgcggcg gatttgccct tggttggtgc gcgccagaag
    31801 ttcggcggtg cgttgctgcg cttgactgta ttggcgcgtg tgttgctgtt gctgctgcat
    31861 cagatttttg tgctgcactt cggacgagcg cacggcagcc tccgcctgtt tgaacgcggc
    31921 ttcggcggcg gaaagtccgg gggcgaggtt ttccagttcg gacgcgatgc cgtcgaggcg
    31981 cgctttttgg gcaatcaggc tttcctgcgc gggcttggca tagagcagga cgctgacttt
    32041 atcgacctga tggccttcgg gtgtgagcca gatttgatgc gtgcccaaat cgctttgatg
    32101 cgcgagggcg tagctcaaat cgggcgcgca caatacgccg tcgagccagt ggtgcaatgc
    32161 cgtctgaaac ggcggctgcg cttggatttg gttcagcaat gcctgtacgg gcagggattt
    32221 tttgatgccg ccggagaggc cgtctgaaag ccatgccgcc tgcccctgcg gcaaaggtgc
    32281 gggcggtacg aaactattcg gcacgctacg ggcgtgcagg cgttcggcaa ggatgacgga
    32341 cagggcgtgc tgccactcgg cgggcgcggt gatgtgttgc cacagttgcg gcgcggcggc
    32401 gtggtcggtt gcctgccaaa agtcggcggc ttcctgctgt tgcgacagga tttgcgacaa
    32461 cgcctgctgc tgcgcctgca aggtaatgtg ttgctgcttc aggctttgga agcggtttga
    32521 ggccgtctga aacgcctcgc gggcggcgtg caaggcttct tcggcggcaa tgatttgttc
    32581 ttcgtaatgc tcttgccggc tttgcaacaa ggcggcggct tcctgagcgg cggcggtttc
    32641 ggcctcgtcg ggcaggttca gggcttggtt ttcctgcttc agacgacctt tgcgctcttc
    32701 gtgcttggca acggtttgtt cggcatgggc aagctgctgc tgcttcaacg ccagttcgcg
    32761 gcggatgcgg tttgcctcgt cctgctgggt ttggaaggca gcgttgagcg tggcttggac
    32821 ttcttccaat tcgggcagac gctcttcgtg ttcggcaacc tgcatcgccc attccgccaa
    32881 ttcggtttgc ttttcttcga cctgcaactc gttttcctca agctgcacgc ggatttgctg
    32941 ctgctcctgc cggatgcgtt gcaactgcgc ctgcgccgcc tgcttgtcgc gttcgatgcg
    33001 ttggtgcagg ttttgccggt ggcggatttg ttcttccaaa cgggcaatct gctcgcgcaa
    33061 cacgccgcgt ttgttgctca attcgtgtac tgcctgctgc tgcgactgtt cggcagtctg
    33121 caaggcgtgt acttcgttat ttaacgcctg aacctgtgcg gcggtttcat cctgctgcgc
    33181 ctgcaaagat tgatgctgcg cggtcgcctt gtcggcggcg gcaagcgatt gctgccattg
    33241 ggcgtaatcg agcaaatcct gttgttgatt caactgcgcg gtcagggatt tgtagcgttc
    33301 ggcggtttcc gcctgttttt ccagcttttc cacctgacgc gccaactcgt tctgcaaatc
    33361 gcccaaacgc tgcaaatgct cgcgcgtgtc tttcagacgg ccttccgtct ccttgcggcg
    33421 ttccttatat ttggacacgc ccgccgcctc ctcgatatag gcgcgcaact cctccggccg
    33481 cgcttcgatg atgcgcgaaa tcatcccctg ctcaatgacg gcataaccgc gcgcgcccac
    33541 gcccgtaccc aagaacaaat cggtaatatc gcggcggcgc acggtctgat tgttgatgaa
    33601 ataagtcgat tccccctgac gcgtcagctg ccgcttgatg ctcacctcgg catactgccc
    33661 ccacgcgccc tgcaaactgt ggtcgctgtt gtcaaatacc agctccaccg aagccctcgg
    33721 cgcaggacgg cgcgtcgccg caccgttaaa aatcacgtcc tgcatactct cgccgcgaag
    33781 ctgcttcgcc gaagcctcgc ccaacaccca gcgcaccgcg tcaatcacat tcgacttgcc
    33841 gcagccgttc ggcccgataa ccgcgacaag ctgccccgga acatgaatcg tggtcgggtc
    33901 ggtaaaagat ttgaagccgg agagtttgat gtgggtcagg cgcataatgg tcggaaaaaa
    33961 taaaaaagaa gcgtatttta acggaaatcc cgccgcacca ccccatatct tgccggcaaa
    34021 accttaccgc atcccgcccc ggtgcggcag gattttgcac atcccggcca tccgtattaa
    34081 aatctttgcg tttcccacac tttcctgaca aatatacgga ggaggattcc atgaaaacaa
    34141 aaacagtcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaaaata gtctaacccg
    34201 cctttcttta atcgctctac ttaatttcca cttcttttta tcctgttcga tcttttgcgg
    34261 ctgcacctca agaaataaac tattcgttat caggcggtaa agtttacgca gataagttca
    34321 gtatcgccat tggggttgag gcaaaagctc agggggataa taccaaactt agcaacctca
    34381 atcggcacat ctgccgaagc aaatgcaccc ggggcattgg ctttaggggg aaattctgaa
    34441 gcatcgaaaa aatttagtat agcagaaggc tatctggcat ctagtgatgg ctatggtgca
    34501 atagcgattg gttctgccgc caagataaag caattggaaa aaggcacaat aaatcatatt
    34561 gtgggaaacg ataataaagg tctttatgtc gatgcagacg gcaatgtaac aaagataacc
    34621 gtaaggactg agtcggaaaa agatattctc tcaagatacg gtcaaactta cggtgcggtg
    34681 gcactagggt ttagatcttc ttcacataat ctttttgcca gttcatttgg agcgttttct
    34741 acagccacag ctattgaaag cttggcagtc ggcgacagca gccaatcaac gggctaccgc
    34801 agtgctactt ttggcagtca cagcagggct ttggcagaag aaagtttggc attaggttat
    34861 gaaactcggg caaatgctta tggttccgtt gctttaggtg cagaatctgt ggcgaatgaa
    34921 gaaaataccg tatcagtggg ttccgataca ttgaaacgga aaatcgtcaa tgtcgccgat
    34981 ggcacggaag atttatgatg cagtaaatgt ccgccagttg aaccgcttaa gcaaacgtac
    35041 aaaccgcgtc ggcgcaagtg ctgcggcgtt ggcttcgtta aaacctgcac aattaggcaa
    35101 aaacgacaaa ttcgcctttt ctttgggctt tggaagctat aaaaatgccc aagcggtggc
    35161 aatgggggcg gtgtttaagc ccgctgaaaa tgtgttgctt aatgtggcgg gcagttttgc
    35221 cggaccgaac cgggctttcg gcgcgggggt ttcttggaaa ttcggcggca aaccgacacc
    35281 tgcggttgcc gcacaaaacg cggcgcatcc tgcaaaagtt ttgcaattgc ggcaggaagt
    35341 ggcggcgttg cgcgcccggc aggcagaaac cgaccgcaaa ctgcacaaac aggccgaaat
    35401 ggaaaacgag ctgcaacagt tgcgccgcgc gttgtccgaa ttgaaaaaac attgaagcaa
    35461 taaacgtaag gcgggacggc ggctctgcct ttttccgatg ccgcacccgc gcgatgccgt
    35521 ctgaagcctt cagacggcat ttttaacgcg cccgaacccc ggttccgcca atgcccgatg
    35581 cttggcgcgg catttgccgt attgccccgc cgccgggcgc gttataatcc gccacgacat
    35641 ccgcccgatt tggaaacctg cctatgcgac cctcctattt catttccgac ctgcatttga
    35701 gcgaaaagca ccccgaactg accgaactgc tgctgcgttt tttacgttct gccgccgccg
    35761 ggcaggcgcg ggcggtttat attttgggcg atttgtttga tttttgggtg ggcgatgacg
    35821 aagtttccga gttgaatact tcggttgcgc gggaaatcag gaaattgtcc gacaaaggcg
    35881 ttgccgtgtt ctttgtcagg ggcaaccgcg atttcctaat cggtcgggat ttttgccggc
    35941 aggcgggtat gacgctgctg ccggattact cggttttgga cttgttcggc agcaacaccc
    36001 tgatttgcca cggcgacaca ttgtgcaccg acgacaaggc atacctgcgt ttccgccgca
    36061 tcgtgcattg caggcggctg caaaaactgt tcctgatgct gcccctgaag tggcgcacgc
    36121 gccttgccgc caaaatcagg cgtgtcagca aaatggaaaa acaggtcaag cccgccgata
    36181 ttatggatgt caatgccgcc tttaccgcgc ggcaggttcg cgcctttaac gcggaaaggc
    36241 tgatacacgg acacacccac cgcgagcata tccatcacga aaacggcttt acccgcatcg
    36301 ttttgggcga ctggcataac gactatgctt caatcctccg cgtggacggg gacggcgcgg
    36361 tattcgtgcc gccggaagaa tgctgaaaat gccgtctgaa gcctttcggg cggctttttc
    36421 tacacgcccc tttcccacac tccctccgcc gcatcatcac catccttaac ccgcattaat
    36481 agccattatc gataaaatgc ttgaccgatt acataagatt acgtaaagtg tgcaaacgca
    36541 taataattgg tcttaccaat ttatctttta accaaaatat cgggtgtgcg ggatttgaac
    36601 gtctttccaa acctcgacgc gccttatgat gcaaatgcct aaaaaggatt cgttatggca
    36661 cttttcctca gcatattccc catcgtcctg ctgatttggc tgatggtgaa aaaaaacagt
    36721 atgccttcct acgtcgcact gccgattacc gcagtgctga tttacgccat caaacttttc
    36781 tacttcggcg atgcgggaat gctgctcaac gccaccgccg cttccggcct cgtcaaaacg
    36841 ctcacgccga ttaccgtgat tttcggcgcg attatgttca accgtatgat ggaaaccacg
    36901 ggctgcatcg atgtcatccg caaatggctg gcgaccatca gcccgaaccc cgtagcgcaa
    36961 ctgatgatta tcggctgggc ttttgccttt atgattgaag gcgcatccgg tttcggcacg
    37021 cctgccgcga ttgccgcgcc gattctgatg agcttgggct tcaatccgtt gaaagtggcg
    37081 attttcactt tggtgatgaa ttccgtgccg gtatcgttcg gcgcggtcgg tacgccgacc
    37141 tggttcggtt tcgcaccgct gaacctgagt gccgaagaca tcctcgccat cggcaggcag
    37201 accggcgtga tgcacttctt cgcaggtttc gtcatccctg tcatcggttt gggcttcatc
    37261 gtgccttggt ctgaaatccg caaaaacttg ggcttcgtcg ccattgccgt cttctcctgc
    37321 accattcctt atgccgcatt ggcgatggtc aacgaagaat tcccgtcgct cgtcgccggc
    37381 gcaatcggcc tgatggtgtc cgtattcgcc gccaaccaag gctggggctt gagcaaagac
    37441 cacgccaaag acccgaatgc cgaaaaagtg ccgttcgccc aagtcgccaa agcactcgcc
    37501 cctttgggta tgctgatcgg catgctggtg gttacccgca tcaaacagct cggaatcaaa
    37561 ggcattttga ccagcaaaga agaatggttc agcttccaac tgccgtttga tttgtccaaa
    37621 attaccgtca gcgactccct gacgattacc ttcggcaata ttttcggaca agatgtcagc
    37681 gcgtcttacc aaacgctgta cgtcccggct tggattccgt tcgtgctgac cgtttggatt
    37741 tgcatcctgc tgtataaaac caaattcaaa gatgcctgga cgatttatgc cgtaaccttc
    37801 aatcaaacca aaaaaccgct gcttgccctg atgggtgcgc tgattatggt tcagctgatg
    37861 ctggtcggcg gcgacaattc gatggtgaaa atcatcggta aggaatttgc cgcaatggcg
    37921 ggcgaacact gggtttattt ttcgccgtat ctgggcgcga tcggtgcgtt cttctccggc
    37981 tccaacaccg tgtccaacct gaccttcggt ccgattcagc agcaaatcgc ccttgatacc
    38041 ggcctgtccg tgaccctgat tctggcgttg cagtccgtcg gcggcgcgat gggcaatatg
    38101 gtgtgcctca acaacatcat cgccgtatgt accgtattgg acgtgaaaaa ttccgaaggt
    38161 gcgattatca agaaaaccgt tatcccgatg gcgatttacg gcgtgattgc cgtcgtcgcg
    38221 gcaatgattt tcttcctcta ggcgaaacgc cgccatccat agcggcagca cgcggggaac
    38281 ggaaaccgcc gctcgtccgg gcggcgcgtc gggcagaata cccgtgccgg aacagcccgc
    38341 cccgccaaac aaatgccgtc tgaaaccgga aaaggcttca gacggcattt ttatcgccgt
    38401 ttgcattcag gcgtagtaat gatgcggcat cttgccgtca aggcggtagc gcgtgccgca
    38461 atacgggcag gcaacgctgc ccgattctcc ttcgcccaaa ggtaaaaaca ccctcggatg
    38521 cccgttccac tgctcgttgt ccggtcccga gcaatacagc ggcagatttt ccggcaacac
    38581 ggaaatttcc tgcggattca gattgtccat ttgattttcc tttgcgtggt tggcgtgcgc
    38641 ccattttacg ccatcggcag gctaaaggat attttcggcg caaagccgca atccgctata
    38701 atccccactt ttcagacggc ataccatgac tgcgcttacc cttcctgaag acatccgcca
    38761 acaagaacca tccgttctgc tctataccct cgtttccgcc tacctcgaac acaccgccca
    38821 aaccggcgac gaatccctct cctgcctgtc cgacgaccag cacacgctga ccgcattctg
    38881 ctacctcgac agccaagtcg aagaaggcgg cttcgtacag cttatcgcct ccggctacgg
    38941 cgaatacatt ttccgcaacc cgcttgccga cagcctgcgc cactggaaaa tcaaagccgt
    39001 gccgaaagtc ttggacaaag ccaaagccct ctacgaacaa cacggcgaaa ccatcgaaac
    39061 gctcgccgac ggaggcgcag acatcgaatc cctgcgcaaa cagttccccg actttgaaga
    39121 atgggacggc gcatactacg aagccgccga acaagacctg cccctgcttg cagaacacat
    39181 cctgtcaaac cgggcggcat tcgcccatat cgggcaggcg tgattgcgtc tgtttccagc
    39241 ccgtgtaaaa cagcgtaaaa tcggcaaacc cgaattatct tccgtcccca tcaaggagca
    39301 agctatgttc ttcaagcaca tcgaagccgc ccccgccgat ccgattctcg gtttgggcga
    39361 agcgttcaaa gccgaaaccc gccccgaaaa agtcaacctc ggcatcggcg tgtacaaaga
    39421 cgcatccggc gcgacaccca ttgtcaaagc cgtcaaagag gctgaaaaac gcctgttgga
    39481 aagcgaaacc accaaaaatt acctgaccat cgacggcgtt gccgactaca acgagcaaac
    39541 ccaaatcctg ctgttcggca aagaccacga aatcatcgcc agccgccgcg ccaaaacagc
    39601 gcaaagcctt ggcggtacag gcgcattgcg tattgcggcc gaatttgcca aacgtcagtt
    39661 gaacgcgcaa accatctgga tttccaatcc gacctggcca aaccacaacg ccattgccaa
    39721 agcggtcggt atccaagaca aaccttatcg ctactatgat gccgccaaac actgcttgga
    39781 ttgggacggc atgattgaag atttgaacca agcgcaaaaa ggcgacatcg tcctgctgca
    39841 cggctgctgc cacaacccta ccggtatcga ccctacgccc gaacaatggg aaactttagc
    39901 aaaactttct gccgaaaaag gctggctgcc attgtttgac tttgcctacc aaggcttcgg
    39961 caacggtttg gaagaagatg cctatggctt gcgcgtgttc ttgaaacaca atacagaatt
    40021 gctgattgcc agctcttatt ccaaaaactt cggcatgtac aacgagcgcg tcggcgcgtt
    40081 cactttggtg gccgaagatg aagaaacagc agcccgcgcc cacagccaag tcaaaaccat
    40141 catccgtacc ttgtattcca acccggcttc acatggtgcg aacaccattg cactggtgtt
    40201 gaaaaatgat gatttgaaag cacaatggat tgccgaactc gatgaaatgc gcggccgcat
    40261 caaagccatg cgccaaaaat ttgtcgagtt gctcaaggcc aaaggttcaa cccaagactt
    40321 tgattttatt attgaacaaa acggtatgtt ctctttcagc ggcttgactc ccgaacaagt
    40381 tgaccgtttg aaaaacgagt ttgccattta tgccgtccgt tccggccgca tcaacgtcgc
    40441 cggcattacc gacgacaaca tcgattatct gtgtgaaagc atcgtgaaag tatagtcaat
    40501 taaaatcaaa ataggacagt agtgcatcgt caaatcgggc gtaatcagac aaaacggttc
    40561 gcagataccg cttaatattc gcccacactt tctcaatcgg gttgggctca ggcgaataag
    40621 gtgcgggagg caatacctta tgtcccaatt tttccgccgt tccccgtaag gcacccatac
    40681 ggcgaaatcg cgcattatct gaaataatca ccgatttttg agccaatgcg ggcagtaggc
    40741 attgctgaaa ccgcgcttca aaaaagaccc cggccaccgt attttgacaa acca
//
LOCUS       DAXFZR010000008        40750 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000008 DAXFZR010000000
VERSION     DAXFZR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40750
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.008"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            17..679
                     /locus_tag="ACFJAS_000313"
     CDS             17..679
                     /locus_tag="ACFJAS_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC3981668.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(764..1615)
                     /gene="lgt"
                     /locus_tag="ACFJAS_000314"
     CDS             complement(764..1615)
                     /gene="lgt"
                     /locus_tag="ACFJAS_000314"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC3981669.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(1689..2819)
                     /locus_tag="ACFJAS_000315"
     CDS             complement(1689..2819)
                     /locus_tag="ACFJAS_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC3981670.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFHCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            2989..3885
                     /gene="argB"
                     /locus_tag="ACFJAS_000316"
     CDS             2989..3885
                     /gene="argB"
                     /locus_tag="ACFJAS_000316"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC3981671.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            complement(4184..4471)
                     /locus_tag="ACFJAS_000317"
     CDS             complement(4184..4471)
                     /locus_tag="ACFJAS_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-activating enzyme"
                     /protein_id="HFC3981672.1"
                     /translation="MPSEARFGVQTALRLKSQKPKATGIHPEKQKTQEFIGKNNNLST
                     VIPTKVGILIRPHGNLYFVIPPIPHHLYRFKSAQKSTSRHSHESGNLGRKI"
     gene            complement(4476..5144)
                     /locus_tag="ACFJAS_000318"
     CDS             complement(4476..5144)
                     /locus_tag="ACFJAS_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC3981673.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(5141..5809)
                     /gene="hda"
                     /locus_tag="ACFJAS_000319"
     CDS             complement(5141..5809)
                     /gene="hda"
                     /locus_tag="ACFJAS_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC3981674.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNNGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(5913..7676)
                     /locus_tag="ACFJAS_000320"
                     /pseudo
     CDS             complement(5913..7676)
                     /locus_tag="ACFJAS_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(8069..8716)
                     /locus_tag="ACFJAS_000321"
     CDS             complement(8069..8716)
                     /locus_tag="ACFJAS_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC3981675.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
     gene            complement(8713..8877)
                     /locus_tag="ACFJAS_000322"
     CDS             complement(8713..8877)
                     /locus_tag="ACFJAS_000322"
                     /inference="COORDINATES: protein motif:HMM:NF027394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC3981676.1"
                     /translation="MNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRLFPESGVFMDF
                     LMKTGKGGKR"
     gene            complement(8879..8956)
                     /locus_tag="ACFJAS_000323"
                     /pseudo
     CDS             complement(8879..8956)
                     /locus_tag="ACFJAS_000323"
                     /inference="COORDINATES: protein motif:HMM:NF027394.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            complement(9090..9761)
                     /locus_tag="ACFJAS_000324"
     CDS             complement(9090..9761)
                     /locus_tag="ACFJAS_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC3981677.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            complement(9927..10646)
                     /locus_tag="ACFJAS_000325"
     CDS             complement(9927..10646)
                     /locus_tag="ACFJAS_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3981678.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCSGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAGSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(10728..12041)
                     /gene="hpnE"
                     /locus_tag="ACFJAS_000326"
     CDS             complement(10728..12041)
                     /gene="hpnE"
                     /locus_tag="ACFJAS_000326"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC3981679.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            complement(12116..12988)
                     /gene="hpnD"
                     /locus_tag="ACFJAS_000327"
     CDS             complement(12116..12988)
                     /gene="hpnD"
                     /locus_tag="ACFJAS_000327"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC3981680.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            13159..15021
                     /gene="hscA"
                     /locus_tag="ACFJAS_000328"
     CDS             13159..15021
                     /gene="hscA"
                     /locus_tag="ACFJAS_000328"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC3981681.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            15207..15855
                     /locus_tag="ACFJAS_000329"
                     /pseudo
     CDS             15207..15855
                     /locus_tag="ACFJAS_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            15891..16643
                     /locus_tag="ACFJAS_000330"
     CDS             15891..16643
                     /locus_tag="ACFJAS_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC3981682.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCVPVG
                     DISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQILPVYEEKEHP
                     ENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLEYGA
                     NPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            16699..17040
                     /gene="fdx"
                     /locus_tag="ACFJAS_000331"
     CDS             16699..17040
                     /gene="fdx"
                     /locus_tag="ACFJAS_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC3981683.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(17100..17640)
                     /locus_tag="ACFJAS_000332"
                     /pseudo
     CDS             complement(17100..17640)
                     /locus_tag="ACFJAS_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            17853..18050
                     /gene="iscX"
                     /locus_tag="ACFJAS_000333"
     CDS             17853..18050
                     /gene="iscX"
                     /locus_tag="ACFJAS_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC3981684.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(18118..18519)
                     /locus_tag="ACFJAS_000334"
     CDS             complement(18118..18519)
                     /locus_tag="ACFJAS_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC3981685.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            18731..19690
                     /locus_tag="ACFJAS_000335"
     CDS             18731..19690
                     /locus_tag="ACFJAS_000335"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC3981686.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            19758..21068
                     /gene="tilS"
                     /locus_tag="ACFJAS_000336"
     CDS             19758..21068
                     /gene="tilS"
                     /locus_tag="ACFJAS_000336"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC3981687.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            21142..21927
                     /locus_tag="ACFJAS_000337"
     CDS             21142..21927
                     /locus_tag="ACFJAS_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC3981688.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADEILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            21937..22134
                     /locus_tag="ACFJAS_000338"
     CDS             21937..22134
                     /locus_tag="ACFJAS_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981689.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            22396..22749
                     /locus_tag="ACFJAS_000339"
     CDS             22396..22749
                     /locus_tag="ACFJAS_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981690.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKYRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEKQ"
     gene            22761..23066
                     /locus_tag="ACFJAS_000340"
     CDS             22761..23066
                     /locus_tag="ACFJAS_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3981691.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            23128..24504
                     /gene="mpl"
                     /locus_tag="ACFJAS_000341"
     CDS             23128..24504
                     /gene="mpl"
                     /locus_tag="ACFJAS_000341"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC3981692.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            24711..25763
                     /gene="bioB"
                     /locus_tag="ACFJAS_000342"
     CDS             24711..25763
                     /gene="bioB"
                     /locus_tag="ACFJAS_000342"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC3981693.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(25859..26632)
                     /locus_tag="ACFJAS_000343"
     CDS             complement(25859..26632)
                     /locus_tag="ACFJAS_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC3981694.1"
                     /translation="MQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHSNGFGAQGIT
                     ATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKFKKLIRK
                     TKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGADVVFGQG
                     AHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNIIPITQVGDK
                     IPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            complement(26670..26972)
                     /locus_tag="ACFJAS_000344"
     CDS             complement(26670..26972)
                     /locus_tag="ACFJAS_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981695.1"
                     /translation="MIINKFTWAMAVFSAILALVIGSGFTALEYVKEPPAAPYAVSAP
                     KTAGVKPRRILPERNPPCCRLTVQTPRPFQTPAMRTDRIRLTERQQIPFPSSGSAA"
     gene            complement(27014..28873)
                     /gene="ilvD"
                     /locus_tag="ACFJAS_000345"
     CDS             complement(27014..28873)
                     /gene="ilvD"
                     /locus_tag="ACFJAS_000345"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC3981696.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEDILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            complement(29046..30817)
                     /gene="cysI"
                     /locus_tag="ACFJAS_000346"
                     /pseudo
     CDS             complement(29046..30817)
                     /gene="cysI"
                     /locus_tag="ACFJAS_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            complement(30844..32637)
                     /locus_tag="ACFJAS_000347"
                     /pseudo
     CDS             complement(30844..32637)
                     /locus_tag="ACFJAS_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
     gene            complement(32634..33716)
                     /locus_tag="ACFJAS_000348"
     CDS             complement(32634..33716)
                     /locus_tag="ACFJAS_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC3981697.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIV
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNSPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVPAKVGQIESVLDVRTLEQEAGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(34145..34801)
                     /locus_tag="ACFJAS_000349"
     CDS             complement(34145..34801)
                     /locus_tag="ACFJAS_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC3981698.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            34866..35756
                     /locus_tag="ACFJAS_000350"
     CDS             34866..35756
                     /locus_tag="ACFJAS_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC3981699.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            35873..37684
                     /gene="typA"
                     /locus_tag="ACFJAS_000351"
     CDS             35873..37684
                     /gene="typA"
                     /locus_tag="ACFJAS_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC3981700.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(38081..38171)
                     /locus_tag="ACFJAS_000352"
     tRNA            complement(38081..38171)
                     /locus_tag="ACFJAS_000352"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38135..38137),aa:Ser,seq:gga)
     gene            complement(38210..38296)
                     /locus_tag="ACFJAS_000353"
     tRNA            complement(38210..38296)
                     /locus_tag="ACFJAS_000353"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38260..38262),aa:Leu,seq:cag)
     gene            38385..>40750
                     /gene="rnr"
                     /locus_tag="ACFJAS_000354"
     CDS             38385..>40750
                     /gene="rnr"
                     /locus_tag="ACFJAS_000354"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC3981701.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEADGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKAAKKIPDHVRKSDLKGRADL
                     CDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVGPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMTVTYAGNVKEYQFYPAVMRSHAR
                     LTYNQVWEWLSDGIGHPFKTQIDTLYKLFKILQKKRLARGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGESGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPIKVKKRKG"
BASE COUNT         9127 a        10475 c        11823 g         9325 t
ORIGIN      
        1 tttgaaaagg aaaatgatgg attcccgaaa atttaccgaa gcatccaaac ggcggttggg
       61 cgaattgttg gatgccaaaa gcgaacaagg caacacgatg cgttgcgacg aggttcaagg
      121 ttttatgacg gcgctgttga gcgggccgga caaattggca ccgctcgact ggctgcccga
      181 agtgttgggc gacgaatcgc aatttaccgc cgccgaacgt tccgaaatcg aacggctggt
      241 tttggcaatg gcgatggaaa caaccgccgc gatgtcggat aaaaagctgc ccgatttgtg
      301 gctctacgac gacggcgagg gcggcagcga tttttacact tggtgcaacg cctatcttta
      361 cggtttggac attgtgccga ccgattggtt tgaagccgcc gatgatgaag cgtttgaaga
      421 gctgttttac cccgtgatgg cattgggcgg catttacgac gaagaggaaa acggcgctat
      481 ccgcctgcaa ttcaccgaag gcgagctggc ggaacttgcg tccgaactgc cttacgcgct
      541 ggcagacatc taccgctact ggcaggcggt catcaacaaa ccgcaaaccg tccgcaggga
      601 aggggaaaaa accggcagga acgatccctg tccgtgcggc agcggcagga aatacaaggc
      661 gtgttgcggt aagaattgaa gcgtttgttt tcatctgaac caaacgtaaa aatgccgtct
      721 gaaaccggat ttccatgttt cagacggtat ttttcacagg cggtcagtgc tgttttttca
      781 tgccgaaccg gacaaagccg acgataccca aaacaatcat cgggacgctc aaccattgcc
      841 ccatcgacag ccccaaggtc agcagcccga gatagtcgtc gggttggcgc gcaaattcgg
      901 caataaagcg gaacacgccg tagccgccga gaaaaagcgc ggcagtctgc ccggtcgggc
      961 gcggtttttt ggaaaacagc caaacgacgg cgaacaggca gatgccttca agggcaaact
     1021 gataaagctg cgagggatga cgcggcagca taccgtattg ttgcagccat tctgcccaaa
     1081 gcggattgtg cgcggcggct tcggcatctt cgtaatgcgc ttgcgggaag cccattgccc
     1141 aaaatgcgtt aatgtcggta atgcgtcccc aaagttcgcc gttgataaag ttgccgatac
     1201 gtcccgaagc gagacccagc ggaacgagcg gcgcgaccgt gtccatcagt ttgaggaagc
     1261 cgatgccgtg cttgcggctg aacaaccata tggcaataac tacacccaaa aagccgccgt
     1321 ggaacgacat tccgccttcc cataccttga aaatatcaag cggatgggcg aggtagtcgg
     1381 agaatttgta aaacaggaca tagcccaagc gtccgcccaa aatcacgccc aaaatgcccc
     1441 atgtcaggaa gtcgtcgagc gattctttgg taaaaacgga caagccttgc gcgatgcgcc
     1501 ttctgccgag aaaggtaaaa agaataaatc cgaggatgta gcttaaggca taccagcgca
     1561 cggcaagcgg gccgatactg atgaggacgg gatcgaattg gtgatggata atcataacgg
     1621 gctttcgttt tcaaatgccg tctgaaagac gcaaaagggc ttgcagacgg cattttcagt
     1681 ggggagtgtc agcgcaaatc gccggcgacg ttcaaaatgg cggaaagcac cgcttcccca
     1741 agccgcaaag aacgctggcc gttccagccg aagtcgtcgt cgggcaggtt ggcgttgtct
     1801 ttgaacggca tttccagcgt gtaggcaagg cagtggaaac ggttgccgac ccagttggtc
     1861 gccaaggtca tattcgcttt gcccggcgcg tctttttcgt aaccgtattc gtcttggaaa
     1921 tcggggctgg cgttcaaaag ggcggttttg aactgcgctt ccaacgcgga aatgcgcgga
     1981 ttgtagttcg gcacaccttc cgttcctgca acaaagacaa agggcagacc ttcatcgccg
     2041 tggatgtcca aaaacaaatc cacgccggtt tccagcattt ttccgcgcac gaagaacact
     2101 tcggggcttt tttccaaagt cgggttttcc cattcgcggt tgaggttcgc accggcggcg
     2161 ttggtacgaa ggttgcccag tgccgaaccg tcgggattca tattggggac gatgtagaac
     2221 gtggcgcggt cgagcagggt gcgtgctgtc gggtcttgcg aatcgagcag cctgccgagc
     2281 agcccttcga caaaccattc cgccatggtt tctcccgggt gctggcgggc ggtaatccag
     2341 attttcatgt cgctttcgac ttggttgcct atggtcagca gattgatgtc gcggccttgc
     2401 acggtgctgc ccaagtcgtc gatgcggcac aggccgctgc cttgtgcgtc gccgaggagg
     2461 ttgaggtgct gctcttcgga atagggttcg aaataggcgt aatacacgct gttggacagc
     2521 ggcgtatgat tgacggtcag tacgccgttt tcgtaggaag tcggtacgcg gaaccagttg
     2581 cggcggtcgt atgaggcaca cgcctgatag ccttcccagc ctttcgggta ggcggcttct
     2641 gccgcgtttt caaaatgcat gatgcagttt tgataggccg cgccttgcag ccggaagtag
     2701 aaccattgtg cgaaatcgga ggcgttgtcg ggacgcaggg cgaggcggat gttggaaggg
     2761 tcggtcaggt ctttgacgac gaccgagccg gcatcgaagc gggtgctgat tttaatcatg
     2821 ggaaagtcct tgctgtcgcc ggtttctcga accggataaa ccgcgatttt accgcccgta
     2881 tcgcaaggct tcaacctgcc cgaaggtgtg ccaaacgccg tctgaagatt gtttcagacg
     2941 gcatttggcg ttaacataag ccgaaattgt caacaatatg gagccgttat ggagtttgaa
     3001 aacattattt ccgccgccga caaggcgcgt atccttgccg aagcactgcc ttacatccgc
     3061 cggttttccg gttcggtcgc cgtcatcaag tatggcggca acgcgatgac cgaacctgcc
     3121 ttgaaagaag ggtttgcccg cgatgtcgtg ctgctgaagc tggtcggcat tcatcccgtc
     3181 atcgttcacg gcggcgggcc gcagatcaat gcgatgcttg aaaaagtcgg caaaaagggc
     3241 gaatttgtcc aaggaatgcg cgttaccgac aaagagacga tggatattgt cgaaatggta
     3301 ttgggcgggc acgtcaacaa ggaaatcgtg tcgatgatta acacatatgg agggcacgcg
     3361 gtcggcgtga gcgggcgcga cgaccatttc attaaggcga agaaactttt ggtcgatacg
     3421 cccgaacaga atagcgtgga catcggacag gtcggtacgg tggaaagcat cgataccggt
     3481 ttggttaaag ggctgataga acgcggctgc attcccgtcg tcgcccccgt cggcgtaggt
     3541 gaaaaaggcg aagcgttcaa catcaacgcc gatttggtgg cagggaaatt ggcggaagaa
     3601 ttgaacgccg aaaaactctt gatgatgacg aatatcgccg gtgtgatgga caaaacgggc
     3661 aatctgctga ccaaactcac gccgaaacgg attgatgggc tgattgccga cggcacgctg
     3721 tatggcggta tgctgccgaa aatcgcttct gcggtcgaag ccgccgccaa cggtgtgaaa
     3781 gccacgcaca tcatcgacgg caggttgccc aacgcgcttt tgctggaaat ctttaccgat
     3841 gccggtatcg ggtcgatgat tttaggcaga ggggaagatg cctgaagcaa agtcggaaaa
     3901 tgccggcttt ggcggaaaac ctgtttgtcc ggtttctgtt tttggggttt cgggcaattt
     3961 ctaagttgtt attcccgagc aaacagaaac cacaacagaa acctaaaatc cgtcattccc
     4021 gccgggaatt tggttttttt gaaatccggt tgttttggat aaattctccg gctttgattt
     4081 tctgtttttc cgataacgcc ataactttga aatttcgtca ttcccgcgca ggcgggaatc
     4141 cagacctgtc ggcacggaaa cttatcggga aaaaaaggtt tctttagatt ttacgtccta
     4201 gattcccact ttcgtgggaa tgacgggatg tggatttttg tgcggatttg aaccggtaaa
     4261 ggtggtgtgg gattggtgga atgacgaaat ataagtttcc gtgcggacgg atcaagattc
     4321 ccactttcgt gggaatgacg gtggaaagat tgttgttttt cccgatgaat tcctgtgttt
     4381 tttgtttttc cggatgaatt cctgtggctt tgggtttttg ggatttcagc ctcaatgccg
     4441 tctgaacgcc gaatcgggct tcagacggca ttgcgtcatt tgaaattcaa aaccggccag
     4501 cctttttctt tggcttcttt ttccagttcg gcatcggggt tgacggcgac gggttcgctg
     4561 acgaggcgca gcagcggcag gtcgtttttg gagtcgctgt aaaaataggt tttgccgtag
     4621 ctttcgagcg tttcgccgcg ttcggcgagc cattggttca gacgggtgat tttgccttct
     4681 ttgaggctgg gcgtgccgat gtaattgccg gtgtagcggc cgtcgggacc ggtttcgagt
     4741 tgtgtgccga tcatgttggc gatgccgaaa aggcggcaga cgggggtgat gatgaactcg
     4801 ttggttgagg aaatcacgag ggtttcgtcg cctgccattt ggtggctctg caccagcata
     4861 cgctgcatag gcgagatgtg ggggatgatg tattccgcca taaattcgcg gtgaaactcc
     4921 gccagctctt ctttgctgta acgggcgagc ggggcgaggt ggagtttgag gaatgcgtcg
     4981 atgtcgaggc agccgttttg gtagtcgcgg tagaattttt cgttttgcgc ttcggtttcg
     5041 gcagcgtcaa ccaacccttt tttgatgagg tattgcggcc aggcgtggtc ggaatcggtg
     5101 ttgatgaggg tgttgtcgag gtcgaagatg gcgaggtttt tcattgggtt tcctgttgtt
     5161 tcaaaagctg gcgaagcagc ggcagggtga tgcgtttgcc catggttacg gcgtagttgt
     5221 ccagcgtgtc gagcatcatc atcaggctgt ccatatcgcg ccgccagtgt ttgagcaggt
     5281 attcgaaaat ttcggaatcg acggttactt ggcgtgccgc cgccatattg gcgagcgcgt
     5341 cgattttttc ttggtcggtt aggggcttga cttcgtaaac gaggcagtat gccatacgcg
     5401 tccgcaaatc ttcgcggatg acgagctgct ggggcgtgta ttccgaaccg agcagcaaaa
     5461 agcctttgcc gttgttgcgg aagcggttga agatggaaaa aagcagggct tgttcttcgt
     5521 tgcccagttt ttcgacttga tcgacggcga ggtattctgc ctcaaacgcg gcatcggtca
     5581 gcggcatgga ggcggcatcg atataggcgg cgtttttgcc ggcttcgagc gcctgtgcga
     5641 cccacgcctg caacagatgg cttttgcccg cgccttcttc gccccagacg tagataaacg
     5701 ggtcgtgttt atgctgtaac acatagacca gttcggcatt ttccgtgccg aggaatttgt
     5761 cgaaactcgg atagtcgtgt gcggcaaagt cgaaaataag ctggttcaca aaggcatacc
     5821 ggatgggttg gaatcgggtt tattgtacgt tgttttcgcg tgtctttcca atttgaacga
     5881 tgccgtctga aaacggcttc agacggcatc gttcagccgc aggcagcgtt gccgacatcg
     5941 aggtgcatat tgcggaacgc gttgagcgta cttcggtgtc cgatactgat gatgctgtcg
     6001 ggcagttttt gctttaaggc gcggtagagc taggcttcgg ccggttcgtc caaggcggcg
     6061 gcttcgtcga gcaggacgat tttgggcttg gagagcaggg cgcggacgaa ggcgacgcgt
     6121 tgcagttcgc cgggggagag tttgtgctgc cagccgtctg ttttatccaa tttgtcaatc
     6181 agataaccca agcggcaggt gttcatggct tcggctaact cgggatgctg tttgtcgata
     6241 tcggggtaac aaaccgcgtc gcgcaggctg ccttgtgccg tgtacgggcg ttgcggcagg
     6301 aagaggatgc cttgatgcgg cggacggctg actttgccgc tgctgccgaa cggccaaagc
     6361 cccgccagcg cgcgcagcag cgaggttttg cctcaaccgc tcgggccgcg tatcagcagg
     6421 gaatcgccgc ttttgagttt gatgttgatg ccgtccaata aaacttcgcc gttgtggcgg
     6481 aacagggcga cgttttccag tgcaatgccg tctgaaactt cgctaatctc gggttgctgc
     6541 gcgccgtgtt gttcttcggt actcagcaaa aagccgtaca gacgctccag tcgggcgtgg
     6601 caggcggtga atttgttgta gaacattcgg aagaaagaca gcgcgttttg cagtcgcgcg
     6661 aaggcttgga cggtctgctg gatgtcgccg attttgattt gtccggcaaa caggcgcgga
     6721 gcttgcaaaa taatcaggaa gagtttgatg ccgttggtga acatatcgtt aaagccgctt
     6781 aagcagacgc tttgccgcgc gatgcgccaa cggttgcgga taatggcttt aaagcggtcg
     6841 gaaagccggc cgtgttcgtg ttgttcgccg ctgtaaaacg ccacgctttc ggcgtggtcg
     6901 cgtacgagga tgagggaata acggtagtcg ccgttgagtt ttttgttttc ataattgtaa
     6961 cgaatcaaag ggttgcctat ccacatggcg ataaagatcg cccaaatcat gaaaataaag
     7021 acaaaccaaa caatgccgtg cggaatgtcg aagccgaaca cggtcaggat gcctgccaag
     7081 ccccacaaaa caacggcaaa ttccagagaa gtaacgaccg aattgaccat gccgcgcaca
     7141 aattcgatgg tcgaagcgat gaattcctgc gcatcctgtt ggatacgctg gtcgatgttg
     7201 tccggcgcgt ggcggcgcat ttgcaggcgg tagtagtttt tgtcggcaag ccagcgcgaa
     7261 gtcaaaactt cattgagccg ttccgaccat ttaatcgcca agccttaatc gagaaagtcg
     7321 ttaacgacgt tgttaaacgc ccgtatcagt accacgccgg cgttcatcgc tgcaaacatc
     7381 caaaatgccg aagcattcaa atcctgcatc gaatcgtaaa gtcctttgga catgaaggta
     7441 ctcaacacat tcaaccgcac ttcggttaac agcagcgtaa ccattgccgt aatcagcagc
     7501 aatactttga ccgtgctttt cggtgtcagg caaaggcgca ggatataggc aaactcccgc
     7561 cccaagcgcg tgttgcgcgc gaaaaacaga atcaccgccg aggcggcggt aatcatcaat
     7621 aaggtctgca acagccaaga cggcgtggaa taaagctcgg tttgccggtt ttgcatggga
     7681 aatctcttac ggtatcaatg ccgtctgaaa aagacgggta cagttgattt tttgatgaag
     7741 tttggggaag ttttgccggt cagggcatat tgcttgttat agtggattaa caaaaatcag
     7801 gacaaggcga cgaagccgca gacagtacag atagtacggc aaggcgaggc aacgccgtac
     7861 cggtttaaat ttaatccact ataatttacc atacaaccac gccggaatta agtttaaatt
     7921 tgaataaaag gttcgggttc tgcaaaatac agaacccgaa ccttgttcgg atattgaaac
     7981 cggtcgcctg attttgggcg gtgcggcttg caagtatcaa gattcgcata tgccgtctga
     8041 agctcggaga ggttcagacg gcatatgctt atttgggctg ctcttcgacg aatctcggac
     8101 ctttcaagat accgttgcgg gaatagggcg acagtaggtt gtatgcggcg gttttggaaa
     8161 cctgataacc gcggtcggtc aggctgttgg caatctgatt gaccactgcg ccgaccaaag
     8221 cccccaacag gccgctgttg ctgttgttgc tgccttcgcg gatgctggcc gaacccgacc
     8281 acaactcttt cccgttgcgg gaatcgacca gccgtgcttt ggcggatacg gtcgtcacgc
     8341 tgtctaaaat ttgatatgaa gtgccgtatt cggtaaccgt aatgtacaaa accgcatcat
     8401 tgccgaaaat ttgatgcagt ttttccggcc ggacggcgtg aatatcggcg gcattggtca
     8461 agccgttttc tttgaaggtt tcctccacga ctgcggcggg gaagacgtaa tagccggctt
     8521 cggaaatcgg cgcggcggtc gaagccagca tcccccaagt gccgttgaca tcaggcgact
     8581 cgttcagcgg cggaaccacc aaaattgaag ccggtttgct ttctttgaat gacgtgtagt
     8641 cgaggtcggg agcttttcga acttggcagg cagacagagc caacacggcg gcaagcccta
     8701 aaatcaaagg tttcatcgct tgcctccttt accggttttc atcaggaagt ccataaatac
     8761 gcccgattcg ggaaacagcc ttttctcttc ttcaaattgg cggaacgcgc cctctttgtc
     8821 tcccgaacgg gaaagcagca gtcccaaatg ggcgtgcgca cccggggcgg cattcatttt
     8881 ttttgttggc ggcttccgca aagtattttt ccatcttttc ggtctgcttg cccaacgaag
     8941 tgtcgtcgtt tttcaaacct tcatagacgg tatcgggata accgccgtaa taatacaggt
     9001 ttttatgccc gccgccggca ggcggtcaga gccaagaccg ccgcacacag cgacaaacgg
     9061 cccaaggttt tcggattcat catttctcct taacggttgg attgccatgc gccgttgtcg
     9121 acagcctgaa ccaagttgtc gacggcttcg cggattgcca agtctaaaac tttgccgttc
     9181 aaagtcgcat cgtagccgga agtgccgccg aaaccgatga tttcgcggtt ggaaagtgcg
     9241 tattcgcccg cgccctgtgt ggaatagacg atttcggaag tattgacgtt gacgatattc
     9301 agagccactt ttgcataggc gatttgcgat ttgccgcgac ccaaaatgcc gaagagctga
     9361 tgatcgccga catctctgcg tccgaattcg gttacatcgc cggtaacgac ataatctgcg
     9421 cctttcaggt tctgcgcttt gccggaaatg ccggattcct gtttcaatgc gctaaggttg
     9481 gtgcggttca gtacgttgaa gcggttggtt tgttgcaggt gtgttaccag gatggttttt
     9541 gcctggctgc ccagacggtc ttcactgtcg gagaaaatgc ctttttggaa gctggagcgg
     9601 ttgtcgaatg ttccgacgga aatcggggtg cgaacaccgt gatattgcgt attgcaggag
     9661 gcgacttttg caacctcgag gctgcgtgag gactcggtcg cacagccggt cagtgaaacg
     9721 gcagcggcgg caaaagcgat ggcggcggaa acggttttca taaaatttac cctaaggtcc
     9781 agttagggaa ataacgggtt atcattattg tccttatgta aatttaagtc aaggtgtttg
     9841 tctgtgcggg acggatgcgc gcggaaggta cggatatttt tcaaaacgga agcgcggcgc
     9901 gggcgggatg cctgcctttt ccggatttag aggtaaacga tttcgccgct ccgccctttg
     9961 ctttcggcac ttgcccacca gacaaatgcg ggcagtacgt ccccgtagct tttgcgttcg
    10021 cttccggctt cgcccggatg ggatttgatc cgttgcgggg aattgatggg gccggggacg
    10081 aggacgttgg cgcgcaggtt gccgaagcgt tcccattcgt cggcggcgac tttgcacagg
    10141 tagttcaacg cggctttgga cgcgccgaag ccgccccagt aggctttggg tgtttcgccg
    10201 tggctttcgc cgacgaagat gacggacgcg tcgggcgact gcttcagcag cgggaacagg
    10261 gcgcgggtca gccccatcgg ggcgacggtg ttgatgcggt attggttgac ccattcggcg
    10321 acggtttgga aatccagcgg cgagagggcg taaaaatagc cggagcagtg gacgatgccg
    10381 tccagtttgc cttgcgtggc ttcggcaatg gtggcggcaa accgttcgaa ttctttttct
    10441 tccgcactca tcaggtcgaa acggatggcg aagggttcgg ggtgtccggc ttcgacaatc
    10501 gcgtcatagg ctttttccag ttttttctga tggcgtgcca ccagaattac ggtcgcccct
    10561 tcggcggcgt aggctttggc gacctgttcg cccaagcctt gcgatgcgcc ggtaactaag
    10621 atggttttgt cggtcagtgt cggcatggtt gtcctttggg tttgacggtt aatgtatttt
    10681 agcgttttgc cgcaccttgt aaagcgtccc gatgccggcc ggcgttttca gacggcatcg
    10741 ttcaggcttt gcaggcaggc ttccgccgac gcgaaacctg attgtacggc ggcttcgagc
    10801 gtggcggggt agtgcgggtg gaggtagtcg ccggcgggga agatacggtg ccggtgcagc
    10861 cacgacaaat ccggcggcgg ggcatcggtt gcggttgtgg cgcgtttttc ggtgatgacg
    10921 cgcacggctt cgggttcgcc caaatgcgga aggatgcgtt tgaggtcggc gtgggctttg
    10981 tccgcccacg cccggtttgc aaacgcgccg acgcggtcgg aaacgctaat gacggcggag
    11041 acttcgtttt cgggcagtcc gagcctgccc cggcaaagca gccattgtgc cgtgccgtcg
    11101 gcaatgccgg tcagcggggc gggcaggcgg acgggttcgg cgtagcgcag atagacggtg
    11161 gtgatggcgt ggtagcgaag gttttgatat gccgtctgaa cgtgttcggg cgtgccttcg
    11221 ggcaggagcg cggcggcgtg gtagggcgcg gtggcaagta tggcggcatc gaaggcttcg
    11281 ccgttgacga ggacttttcc gtccgggagg gtgttcagac ggcatacgcg cgtttcgagg
    11341 cggatgtccg cgccgagccg ttgaagctcc gccaaggcgg gttcggcgac gattgcgccc
    11401 aaatcctgtt tgggtaggag atagtcgctg ccggattttt tcgtcagcac gccgtcggac
    11461 aaaacgttgc acaacacgcg caggcttgcg gtttccaaag gcgtgttgag cgcgccccag
    11521 accaagggct gccaaaactg cattacggcg gcacgcggca cgttccgctg tttcagccat
    11581 tgtgcaactg tcgtgtcggg ctgtccgagg cgtgcggact tctgcaaatc ggacatatcg
    11641 gcaagcagtt tggctttgaa tgcggacggt acgcgccggg caagcagcac gccgcccaaa
    11701 atatgcagcg gcgcgggcag ggggagggcg cggaactgca aaccgccgtg catatgccag
    11761 tgcagcggta cgcgcaaaaa ggcggcacgg gggtctgaac cgatggtttt catcaggcgc
    11821 aacacgcccc ggtatgcgcc gagcaaaatg tgctgcccgt tgtccaaaaa accgaaaccg
    11881 tcggtatttc cggccagtgt gcgcgccctt ccgcccgcct gccggccggc ttcaaacagg
    11941 gtaacgtcgg cgtgccgcgc caaggtgacg gcggcggaca agccggccca gcctgcgccg
    12001 atgacggcga ttttcgggcg cggatgcggc gtgttcatca tttattcctc caatggttga
    12061 aaccccgttt ttcggggcgg cgggggttgg gttttcctga agggcagtat gcgccttatg
    12121 cccgttccgg cgtgccgggg cgcggtttga atccgaataa ccaggttttc agggcaatgc
    12181 gttttttgcg cggcgaaggg agggcgattt tgtatttgag gacgttttgc gcgccgtctc
    12241 ggtcgatttc gttcaacagc gcgtaataaa ccgccgccat aaccagtccg actttttggg
    12301 cttttttatc ggcatcaggc agcagcgata cggcttcgcg gtaggtttca cgggcgcgtt
    12361 tgatttggaa cgccatcaat tcggcaaaat tgcccgtcgg gctgcattgc aaaatcacgc
    12421 ttgcgggtac gtcaaaccgc tgcatttcct ccatcggcag gtaaatccgt cccctgcgcg
    12481 catcttcgcc gacatcgcgg atgatgttgg tcagttgcag cgcaagaccc attttatccg
    12541 cgtattccag cgttttgccg tctgaaaacc ccaaaatccg cgcaatcagg cagccgacca
    12601 cgcccgcgac gcggcggcaa tacagtttca attcctcaaa actgccgtaa cgcgcctgaa
    12661 ccaaatccat ctgcatcccg tcgattaagg cttccagttc atatttcggc agcttgaagg
    12721 tttccttaac ctgccgcaag gcctgattga cggggtgttc cggcatcgcg ccgccgaacg
    12781 cattgtccaa atcgccgcgc caccagttca atgttgcctg tgcaacatcg gggttggaac
    12841 attcgtcaac cacatcgtcc aattcgcggc aaaaggcata cagaaccgtt accgcatcct
    12901 gtttttcctg agtcaggaaa cggaagcccg acaaaaaact ggagcggctt tcctctgctt
    12961 tttggcggca atagtcgagt cctttcacga tttatattcc taatgatggg cgggaaaggc
    13021 ggattttatc ggtatttggc ggtagagggc aatttcggcg gcacggccca atccttagcg
    13081 gtttgctcaa ctatcggcgc aaattctgtt aaaatgccgc cttccttcct ttacacaccg
    13141 caccgacagg caaaatttat ggctcttttg cagatttcag aaccgggtat gtccgccgcc
    13201 ccgcaccggc accgtttggc ggcaggcatc gatttgggta cgaccaacag cttggtcgcc
    13261 accgtccgca gcggcagtgc cgcctgcctg cctgatgccg acgggcgcgt taccctgcct
    13321 tccgtcgtcc gctatctgga aaacggcggc attgaagtcg gcaaaaccgc cctgtccgcc
    13381 caaaaaaccg acccgctgaa caccgtcagc tccgccaaac gccttatcgg gcggactctt
    13441 gccgatctgc atcaaaatac gcattacctg ccttaccgtt tcggcgacaa tcaacgcgtt
    13501 atcgaactgc atacgcggca gggggtgaaa acgcctgtcg aagtgtcggc ggaaatcctc
    13561 aaaaccctca aattgcgtgc cgaagaaacc ttgggcggcg atttggtcgg cgtggtgatt
    13621 accgtccccg cctatttcga cgatgcgcaa cgccaggcca ccaaagatgc cgcgcgtctg
    13681 gcgggtttga acgtattgcg cctgctcaac gaacctaccg ccgccgcaat cgcctacggg
    13741 ctggacaacg cctcggaagg cacgtttgtc gtgtacgatt tgggcggcgg cacattcgac
    13801 gtatccgtat tgcaactgac caaaggactg tttgaagtca aagccaccgg cggcaacagc
    13861 gcgttgggcg gcgacgattt cgaccaccgc ctgttctgcc gcctgctcga acaaaacaga
    13921 ctctcccaac tcaacgaaca agacagccaa ctcctgctct cgctcgtccg cgccgccaaa
    13981 gaacaattga ccacgcaaac cgaagcgcgc attcaggcga cgctttcaga cggtatggca
    14041 atcgacacaa gcatcagtcg cgccgagttc cacaacctga cgcagcattt ggtgatgaaa
    14101 acgctcgagc cggtcaaaca ggcgttgaaa gatgccggtg tcggtaaaaa cgaagtcaaa
    14161 ggcgtggtta tggtcggcgg ttcgacccgt atgccgcacg tccaacaggc agtcgccacc
    14221 tttttcggac aaaccccgct gaacaacctc aatcccgacg aagtcgtcgc gctcggcgca
    14281 gccatacagg caaacgtcct cgcaggcaac aaagccgacg gcgaatggct gctgttggac
    14341 gttaccccct tgtcgctcgg tttggaaacc tacggcggct tggcggaaaa aatcatcccg
    14401 cgcaattcca ccatccccac cgcgcgcgcg caggacttta ccaccttcaa agacggtcag
    14461 accgcgatga cgatacacgt cgtgcaaggc gagcgcgagc tcgtttccga ctgccgcagc
    14521 cttgccaaat ttaccctgcg cggcattccg cctatggcgg cgggtgcggc gcgtatccgc
    14581 gttaccttcc aaatcgatgc cgacggtttg ctgtccgttt ccgcccaaga acaaagcacc
    14641 ggcgtacagg cgcaaatcga agtcaaaccc tcctacggtt tggacgatga caccatcacc
    14701 caaatgctca aagacagcat gggcaacgcc gccgaagata tggcggcccg cgcccgcgcc
    14761 gaagccgtgg tcgaagccga aagcctgacc gatgccgtca acgccgccct cgagttggac
    14821 agcgatttgc tggatgccga agagtttgca caaatccagc gagacatcgc cgatttgcaa
    14881 ggccgtctga aagacggtaa agctgaagac atccgtgcag ctgtcgccaa actcagccgc
    14941 agcaccgaca atttcgccgc caagcgcatg aaccgcaaca tccaacgtgc gctgacaggt
    15001 cagagtgtcg ataatatttg atacttaaac ggtttcagac ggcatagata taacctgatg
    15061 ccgtctgaag gatcgaaaac acttgaaaaa catcgatatg gaaaagtcag gcattgtcta
    15121 ttcgatgaaa accgtcatca agggcgtgta tagtgaatta aatttaaacc ggtacggcgt
    15181 tggctcgcct tgccgtacta tttgtactgt ctgcggctcg ccgccttgtc ctgatttttg
    15241 ttaattcact atataaaatc ggcatttcgg acgtaagcaa ttttgaaggc agaatgcgcc
    15301 atttggaaaa caacggctat gcgaacgttg ccgggttgga acgcctcctc gccgtcaaaa
    15361 ccgacaatta caaagaaaaa aaacctgctc catgaaattt tcagcaaaag caggataggc
    15421 gataccgaat tgttcgccgt ggacgaaaac cttgtgaaac gtttgttttt atcgcttcgc
    15481 ggcgaaatcg tgttcccgaa aaacgaaacg gcggaatcgg aatttgaaaa aagcgtccac
    15541 gaacgccggc aggaagggaa tgccgggtcc ggccgcaaac aactgcttga tttggtacgg
    15601 cgcggacacc gggaataccc ttacgcgctg ccccggcttt tggcggacgc ggcatcctac
    15661 aagccgaaaa aatcgaaaat ccgccttttt aaagaagcat attcccgcaa aagcggcagg
    15721 aggctgaccg acgaaattgc agacggcatc catatttaca cctggttttc gcgggtaaac
    15781 ttagaaaaag cctattccga atatttggaa cttttcaaat ccgaatcgga tgccggaggc
    15841 agaaaaccgc ggtaaggtgc aaacagatac cgtacacgtt gaggagcagt atgatgggcg
    15901 attccgtcat ttattatgta gaacaggcag acgaaccggt aaaccgtgcc ggcgaacgcg
    15961 cccgtaaaac attcaaatac ttttggcgcg agcttttttg ggaacgccgc cgcattattt
    16021 ccgccttgga ttttgccatg gtcaaagtcc cttttttcca agacggcgaa atttgcgaac
    16081 atatgtggat cgacgatatt tattttgacg gtctttacat ttacggtgtg ctgaacaatg
    16141 aacccggcgg actgaccaat gtcgaacaag gcgaaagcgt ttgcgttccg gttggcgaca
    16201 tcagcgactg gatgttcgtg tgcaacggca tcccctacgg cggctttacc gtacaggcaa
    16261 tgcgcggaca gatgacggaa gaggagcgta ccgaacacga taccgcctgg ggaatcgatt
    16321 tcggcgatcc cggacagata ttgccggtgt atgaagaaaa agaacatccc gaaaatctgg
    16381 aagagcatcc gatgtgccgg aactgtattg acgattttcg gcaacagttg tcccaaaatc
    16441 cggattttct gcatgagcag gacgaagacg gctatacgcc gcttcatcat gaagccatgg
    16501 caggaaatgc acttatggtt caagccatgc ttgaatacgg cgcaaatcct gcctcaaaga
    16561 catcggaagg ctataccgcc ctcgattttg cccgcctgac gggctggcaa aatgttgccg
    16621 acctgctcga accgcgacat taggcagaca gttttcctaa aaacggacac aaatactttt
    16681 tacagaaaga caataaaaat gcccaaaatc accgtacttc cacacgcaac attatgcccc
    16741 gagggtgcag tcatcgataa cgcgcccgaa ggcaaaaccg tcctcgacgt gctgctcgac
    16801 cacgacatcg aagtcgatca cgcctgcgaa aaatcctgcg cctgcaccac ctgccacgtg
    16861 attatccgca aaggtttcga cagtttggaa gagccggccg aattggaaga agacctgctc
    16921 gatcaggctt ggggtttgga agccgattcg cgcctgagtt gtcaggcggt tgttgccggc
    16981 gaggatttga ttgtggaaat ccccaaatac accatcaacc acgcgcgcga agaacactga
    17041 aaacaggccg tctgaagccg gcacgcttca gacggcattg ttgcgcggat aaggcgcaat
    17101 taccgaaaaa cagacgtttg tgaagacgga actttcgatt ctatattgat ttttatcggt
    17161 tttttgacgg aacgacccga tacggcatct gaggcggctt gcgtttcttc ctgcccgcct
    17221 gccggatttg cctgtccttg cgcgaaaccg aaagagacgg cggcgcggcg gacaaggtcg
    17281 agatagcgtc cttcaagctc ggggcaggcg gcggacacgc tttctgccgt aaccgtgaag
    17341 ccgcccgacc gactcggaaa ggcgttccgc aatctgcatg cagatgcgcc aacgccgggc
    17401 gcgcaacatt gccgcatcgc aaccgacagc cgctttatcc aaagcaatca gaccgcgcct
    17461 gctccggtag ttaaggcaaa ccgttactct gtagagggta tgttgcccgt ccaatatttg
    17521 atacagtttg cgtatcagca gaatcaggcg gtcaaactcc tccatctccg acagggagtc
    17581 gggatggtcg gaagcggtat aaaccagttc ggacaatttt gcggcaaaca tattgttcat
    17641 caatcttcct tatcggttga aaccccgctc ttcagggcgg tagaatcaga tttgtttggg
    17701 aggggtaact cctcccggat caggacgaca cataaggtgg tgcttgatgt gccgtccggc
    17761 gagttgaaac attcagccat cctcaagggg cggcagtttt gccgaaacat attctacacg
    17821 gcttcaatgc cggacgataa aaggaaattt atatgaaatg gaccgatacc cggcgcatcg
    17881 ccgaagaact ctacgacctg cacggcgaag ccatcgatcc caaaaccgtg cgctttaccc
    17941 aactgcgcga cctgattatg gcattgcccg aatttgacga cgatcccgcc cgttgcggcg
    18001 aacgcatcct tgaagccgtg cagcaggcat ggatagacga ggcggaataa gtttcaggaa
    18061 tgccgtctga aatgcggcgg tacgcggttc gtgcttctgt ttgcagcggg aatggtttta
    18121 ccagtctcct tttttcagcc tgtccagttg gcggcggtcg cgcttggtcg gtctgccgtc
    18181 gggatgggcg gaagtgatgc ggctgaattg gtcgagctgt ttgcactctt ccctcaatgc
    18241 tgccgttttc gcgtcttctt catacagaag ccgcgcctcg ggtgccgggc ggcgttggtg
    18301 gttcaaacct ttaaccttga ttttatgggg aagggaattg agcgtcaggt cgataatatc
    18361 gccgatgtct atggttttac tgtttttgac tttcgagccg tttacttgaa ccctacccag
    18421 ttcgatatgc ttttgcgcaa gggaacgggt cttgaaaaaa cgtgccgccc aaagccattt
    18481 gtccagccgc atggcggaag aatcgtgctt gtctttcata cgattttgtt tgaaataatt
    18541 gaatttgttt cgagtttagc ataagataca tcgccttata actagtatat atgcactaat
    18601 ccgctgtttt ccatgctgtc cgaacacaaa aagagggtgt ggaaaagccg ttttggacaa
    18661 taaattaact gcggaatatg cacaaatagc gtatgatagc ggcagaatct gttgatgaga
    18721 gcttcatttt atgaaacctg tttttttgga ttttgaacaa cccatagccg aactgaccaa
    18781 caaaatcgat gagctgcgtt tcgtccaaga cgagtctgcc gtcgatattt cggacgaaat
    18841 acaccgtttg cagaaaaaaa gcaacgattt gaccaaatcg attttcagca aactcacacc
    18901 cgctcaaatt tcacaggttt cccggcatcc gcagcgtccc tatactttgg attacattga
    18961 cgcgctgttt accgattttg aagagctgca cggcgaccgc cactttgccg acgatcatgc
    19021 gattgtcggc ggattggcgc gtttcaacgg acaaagcgtt gtggtcgtcg ggcatcagaa
    19081 agggcgcgac accaaagaaa aaatccgccg caacttcggt atgccccgtc ctgaaggcta
    19141 ccgcaaagcc ctgcgcctga tgaaaacggc ggaaaaattc ggcttgcccg tcatgacctt
    19201 tatcgatacg ccgggcgcgt atcccggcat cggcgcggaa gaacgcgggc agtcggaagc
    19261 catcggcaaa aacctgtacg aactgacccg actgcgcgtt cccgttttgt gtaccgtcat
    19321 cggcgaaggc ggttcgggcg gtgcgttggc ggtcgccgta ggcgattacg tcaatatgct
    19381 gcaatattcg acctattccg ttatttcccc cgaaggctgt gcttcgattt tgtggaaaac
    19441 cgccgaaaag gcggctgatg cggcacaggc tttgggtatt actgccgacc gcctgcaaaa
    19501 gctggacttg gtcgataccg tcattaaaga gcctttgggt ggtgcgcata gggatttcgg
    19561 gcaaaccatg aaaaacgtaa aagccgtttt ggaaaaacaa ctgcacgaag cgcaaagcat
    19621 cccgcttgcc gatttgcttt cgcgccgttt cgaccgcatt atggcttacg gcaaattttc
    19681 ggaacaataa ttcgggtaga acaagcagca agcagtttgt ctgaaactgc ttgctttttc
    19741 tttatcggga tgaagccgtg ctgactctag atgcgtttga gcaatgcttg aaggattgtt
    19801 ttcctcaagg tctgaatgga aaaaaaacag cagtggcatt aagcggcggc ttggattccg
    19861 tcgttttgct gcatctgctg gtttgtgccg ggaagcgggc aggatttgtg ccggaggcat
    19921 tgcacatcca tcacggcttg agtccccgag ccgacgattg ggcggatttc tgccggaatt
    19981 attgcgatat gctaggggtg gggctggaaa cggttaaggt ctgcgtggaa aaaaacggtt
    20041 tgggcatcga ggcggcggca aggcaaaagc gttatgccga gtttgccgaa aaaggctttg
    20101 acgttttggc gttggcgcac cacagggacg atcaaatcga aacctttatg ctggcagtcg
    20161 cgcgcggcgg aggtttgcgc gctttggcgg ctatgcccgc cgtccgccct ttgggggaaa
    20221 acggcattat ctggcggcct ttgctgcctt tttcgcgcca agacatatgg gattatgccc
    20281 gaaaacacgg tttgccgaat atcgaggatg aaagcaatac cgatacggct tatttgcgaa
    20341 accgcttccg gcaccgtatt ttgcccgaac tttcggcgca gattccccat ttcgggcggc
    20401 atgtgctgaa caatgtccgc gctttgcagg aagatttggc tttgttggaa gaggtcgtcg
    20461 ttcaagactg ccgttgggtt tgcggggccg gttatttcga tacggcgcgg tggctgacgt
    20521 tttccccgcg ccggaaaacc catattttgc ggaattttct aaaggaaaac ggcatccccg
    20581 tgccgaatca gaacgcactt gccgacattg cccgcgtttt gacggaggca aaaaccggac
    20641 gttggaactt gcaaggcttt gaattgcatc attatgcagg caggctgttt gtgtttgcgt
    20701 cggaacagtt ggcaaaaccg gcttttctga aagatgggac gataagcgga aatctcaaaa
    20761 aaatactgac agaacataga tttgtgttga agcggcatcc gttcggactt cctgaagcca
    20821 tgttggaaca ggacgggatt ttgaggacgg tagcggcatc ggatacgttg gctgtcggcg
    20881 gcatccataa aaatgtgaaa aaaatccttc aggggaaacg ggttctgcct ttcctgcgcc
    20941 caatttggcc gctcgttgcc gacagcggaa accgtccatt ggcgttggca aactgttgtg
    21001 cggattttca aatctcggtt tcagacggca ttttgcccgt acatcctgac tttcccattt
    21061 tattttgata atatcgcaaa cagatttcgg cggcgttcag tcgggtattg tccggttgca
    21121 tatttctaaa aggcttgtga agtgaaacac atcagttcga ccaataatga acatatcaga
    21181 cacctgcacc gcctgttgtc gcaaggaaag ttcagacggc agtatgccca aaccgttttg
    21241 gagggcgtgc acctgcttca ggttttcctg caatccggca gaaagccggt cggggtatat
    21301 attcccgaag ctaaaatgcc gtctgaagaa gtccttaaat tgacagcggt tttgccggaa
    21361 gacgggattt tttccgtttc agacggtata ttgaaaaaaa tcagcagctt gagttgtgca
    21421 gacgagatac ttacactgat tgatatccca ttgggcggaa ccttgccgga caaaggcgac
    21481 tgtgcggttt tggacggcgt gcaagatccg ggcaatgtcg gcaccgtctt acggagtgcg
    21541 gcggcggcag gggtaggtac ggtcgttttg ggcaggggtt gtgcggacgc gtggtcgccc
    21601 aaggtattac gcgcgggtat gggcgcgcat ttcttgttgg acatttattc gcaggcggat
    21661 ttggaaatat ggttggcgca ctatgaagac cgtgtgtttg ccaccgcgtt gcgcgaggaa
    21721 aagcaggcgg ttttgtacgg cgaagatttg tgcgaaccga cagcttgggt gttcggcaac
    21781 gaaggtgcgg gtgtcggtaa agcagtttta gacagggcgg acaagtgtgt caggataccg
    21841 atgcacgatg caaccgagtc tttaaatgtc gcgatggcgg cgacaatctg cctgtttgaa
    21901 cagatgcgcc aacgggcggc gtattgagga agagaaatgc cgtctgaaaa aatctattac
    21961 ggcgtattga ttttcttatg tatcgcttct atgctgctgt caccgttttt ttatgcgggt
    22021 gctttgaagc ccaagaaggc ggcattgcgg aaggacgggc agtggaaact catcttatcg
    22081 tccaatgccg tggcggcggc ggttttgttt tggatatggt ggaaatggtt ttgacagata
    22141 ttgctcaaaa tcgtgctaat ggaatccgaa caaataaaga atttggtaaa aaatttgtta
    22201 aatcaacgga ttaaagtttt gtggaaaaca aaacagctct aagcaaatag ggcgtttgtc
    22261 ggtaaatacg gaagagttgc ggcattatcg ggcatcttta acaagtagtg tcggcttggc
    22321 aggcaatcgg tttttatggg cagcttgcaa aatcgcggat ataaaattgc gaatcggtta
    22381 aagtgtgggg acgctatgaa aaattgcgaa tttttttatg atccgacaag ggcaatctat
    22441 gatagcggtg cagattactt aactagggaa aaatatagat tagtcgtaat cgcaaatagc
    22501 gcttgggggc tattgctgaa tttaccttgt tattatgacg aggttttgga aaagcggaaa
    22561 ataccgttcg gcaaacagga aattgatgac gatatggaca aagtgtccgc ccttaagcgg
    22621 aagtttaaag acatttctga aatcaaagta ggggatggtt gggaataccc gttcaattat
    22681 gagcaaggaa tgaaagaatt agatgaagtt ctattgaaat acattccttt ttttgaagag
    22741 aaacaataaa ggagtttaat atgcgcgtat ctaaaatgat tggaagtata ttgcttgttg
    22801 cagcggttca gaccgtattt tcggcaaatg tttacgagtg ccgccataat ggtaaaacca
    22861 gttacagcca aactccggga aaacattgta ccaacgcggg tttggggcgg gatcgggtgt
    22921 acagttcggt caggcctgcc gtgaaagaca gggcggaaga cgcaggagtt ggcgattatt
    22981 cggacacggt gagggacgaa gccgtccaaa atccgaaagg gaatgcacag aaagacggtt
    23041 cggatgccgg catcagaccg cattgattga aaccgaatca accattacgg tgttgaacaa
    23101 tagaatttga acgattaggg aactctgatg aaacacatcc acattatcgg tatcggcggc
    23161 acgtttatgg gcgggattgc cgccattgcc aaagaagccg ggttcaaagt cagcggttgc
    23221 gacgcgaaga tgtatccgcc gatgagcacc cagctcgaag ccttgggcat aggcgtacac
    23281 gagggcttcg atgccgcgca gttggaggaa tttcaagccg atatttacgt catcggcaat
    23341 gtcgccaggc gcgggatgga tgtggtcgag gcgattttga accgtgggct gccttatatt
    23401 tccggcccgc aatggctggc tgaaaacgtg ctgcaccatc attgggtact cggcgtggca
    23461 gggacgcacg gcaaaacgac caccgcgtcc atgctcgcct gggtcttgga atatgccgga
    23521 ctcgcgccgg gcttcctcat cggcggtgta ccggaaaatt tcggcgtttc cgcccgccta
    23581 ccgcaaacgc cgcgtcaaga cccgaacagc aaatcgccgt ttttcgtcat cgaagccgac
    23641 gaatacgaca ccgccttttt cgacaaacgc tccaaattcg tgcattatcg cccgcgtacc
    23701 gccgtgttga acaatctgga attcgaccac gccgacatct tcgccgactt gggcgcgata
    23761 cagacccagt tccaccacct cgtgcgcacc gtaccatccg aaggcctcat cgtctgcaac
    23821 ggacagcagc aaagcctgca agatactttg gacaaaggct gctggacgcc ggtggaaaaa
    23881 ttcggcaccg gacacggctg gcagattggt gaagtcaatg ccgacggctc gttcgacgta
    23941 ttgcttgacg gcaaaaaagc cggacacgtc gcatgggatt tgatgggcgg acacaaccgc
    24001 atgaacgcgc tcgccgtcat cgctgccgca cgccatgccg gagtcgatgt tcagacggcc
    24061 tgcgaagcct tgggtgcgtt taaaaacgtc aaacgccgca tggaaatcaa aggcacggca
    24121 aacggcatca ccgtttacga cgatttcgcc caccacccga ccgccatcga aaccacgatt
    24181 caaggtttgc gccaacgtgt cggcggcgcg cgcatcctcg ccgtcctcga gccgcgttcc
    24241 aacaccatga aactcggcac gatgaagtcc gccctgcccg caagcctcaa agaagccgac
    24301 caagtgttct gctacgccgg cggcgcggac tgggacgttg ccgaagccct cgcgcctttg
    24361 ggctgcaggc tgcgcgtcgg taaagatttc gataccttcg ttgccgaaat tgtgaaaaac
    24421 gcccgaaccg gcgaccatat tttggtgatg agcaacggcg gtttcggcgg aatacacacc
    24481 aaactgctgg acgctttgag atagcccggg caatgccgtc tgaaagccct ttagacggca
    24541 ttgcccggct gcgcggcaca aaggcggaaa aaccgtttgc cccgtatttt caaacgcgtt
    24601 acacttgccg ccgctgtttt cagccatttg attacccgca accgccgtca ttgcgccggc
    24661 ggtttgcctg tcagcgtcat tgcgccgctg taaatacgaa agaacacatt atgaccgtat
    24721 cccccgtcgc cttgcgccgt aagaccgagt gcaagcctca tcccaccgcg cgctattgga
    24781 aaaaatgcga tgtcgaggca cttttcggac tgccgttttt agaacttgtc tatcaggcgg
    24841 cagaagtcca ccgccaaaat ttcaacccgc gcgaaatcca gctttccacg ctgttgtcca
    24901 tcaaaaccgg cggttgtccc gaagattgcg cctactgtcc gcaatcagcg caccacaaca
    24961 ccaatttggg caaagagcag atgatggatg tggatgaaat cgtcgaaaaa gccaaaatcg
    25021 ccaaatcgcg cggcgcaagc cggttttgta tgggcgcggc atggcgcggc cccaaaccca
    25081 aagacgtgga gacggtttcc gcaatcatca aagccgtcaa gggcttaggt atggaaacct
    25141 gcggcacgtt cggtatgctc gaagaaggta tggcggaaga cttgaaagag gcgggcttgg
    25201 attattacaa ccacaacctc gacaccgacc ccgaccgcta caacgacatc atccacacac
    25261 gccggcacga agaccgtatg gataccttgg gcaaagtccg caacgccggt ttgaaagtct
    25321 gctgcggcgg catcgtcggg atgaacgaaa cccgcgccga acgcgccggg ctgattgcca
    25381 gcctcgccaa cctcgacccg cagcccgaaa gcgtgccgat taaccggttg gtcaaagtgg
    25441 aaggcacgcc gcttgccgat gccgaagatt tggattggac cgaatttgtc cgtacggttt
    25501 ccgtagcgcg gattacgatg ccgcaaagtt atgtccgcct ctcggcaggg cgcagcaata
    25561 tgcctgaagc gatgcaggcg atgtgcttca tggcgggtgc gaactcgatt ttttacggcg
    25621 acaagctgtt gaccacaggc aatcctgatg aggacggcga tagaatcctg atggaaaagc
    25681 ttaacctgta tcccttgcag tttgagccgg aaggcgaggt cgccgaagtg gaaaaagcct
    25741 ccgggattaa agcggattat tgacgattga aaaatgccgt ctgaaacttg aatgccgtct
    25801 gaaacctgaa aaaaggcttt cagacggcat ttgttcggac ggaatttcca atatcttttt
    25861 accggcgcgt gatgtcgccg ccgggcgaga catccagtcc gttcccgttg gggaagtcgc
    25921 tatggttcag ataaataatc cgctcaataa cagttttttc agggtcgatt ttggggattt
    25981 tatcgccgac ttgagtgatg gggataatgt tgccggaaac gaaccgcccc tgtttgtcgg
    26041 tgataatttt gaaaatcggc gcaatgccgc tgatgccgta ggtggcaaag ttgccgccgc
    26101 tgtaagagat gaagcggtcg tggtaaagtt cgacggcgcg agtaacgtgc gccccctgcc
    26161 cgaatacgac atccgcgccg gaatcgacgg cgagccgcgc aaactcgacg acgttgcccc
    26221 tgttttcccc atagaagatt tcggtatcga acggcaggtg ttcagcctgt ttcccttccg
    26281 cgccgccgtg gaacatcaca atgacgatgt cggttttttg tttggttttc cgaatcagtt
    26341 ttttgaattt ggcgtaatcg ttcagtttga cggcggcaag gttgggggcg aaggagacga
    26401 agccgtatct cacgccgttt ttcttcagga tggcggtttc aaacctgttt tcgatgcccg
    26461 aatatttgat gttcaattcg tcaaggttgc ctgccgttgc cgtgatgcct tgcgcgccga
    26521 agccgttgct gtggttgttt gcgaggctga ggtagtcgaa tcccgcgtcg gcaaggtatt
    26581 gcccgtatgc ggagggcgtt cggaatgcat agcatatttt ggtggtttgc acattttttc
    26641 ggcgtaccgc cttcgtcaaa cagcgtacct tatgccgccg accccgatga tggaaacggt
    26701 atctgctgcc tttccgtcag gcgtatcctg tccgtccgca tcgccggtgt ttgaaacggg
    26761 cgcggggttt gaaccgtcaa tcggcagcac gggggattcc gttccggcag gatgcgccga
    26821 ggcttgaccc cggcggtttt cggcgcggaa acagcataag gggcggcagg cggctctttt
    26881 acatactcga gcgcggtaaa gccgctgccg ataacgaggg ccaaaattgc ggaaaatacc
    26941 gccatcgccc aggtaaattt gttaataatc atacctttac tgttcagacg gcatttgccg
    27001 cacgttttgg ggcttatctc tcgatttgcg atacgtcgcg caccgcgcct ttgtcggcgg
    27061 aagtcgccat cgcgccgtaa gcccttaatg cggcggagac gtagcggtcg cggttttccg
    27121 gtttccacgc tttgctgccg cgagcttcca tttcggcacg gcgtgcggca agctcttcat
    27181 cggaaatgac aaggcggatg ctgcgtttgg ggatgtcgat ttcaatcgta tcgccttcgt
    27241 gtaccaaacc gattgcgccg ccttccgccg cttcgggcga ggcgtgtccg atggacaaac
    27301 ctgatgtgcc gccggagaag cgtccgtcgg ttaagagggc gcaggctttg cccaagcctt
    27361 tggatttcag gtaggaggtc gggtatagca tttcctgcat acccgggccg cctttcgggc
    27421 cttcgtagcg gatgatgacg atgttgccgg cgacgatttg gttgcccaaa atatcttcga
    27481 cggcggcttc ctggctttca aacacgcgcg cgcggccggt gaatttgagg atgctctcgt
    27541 ccacgcctgc ggtttttacc acgcagccgc gctcggcgat gttgccgaat aagaccgcca
    27601 aaccgccgtc ttgcgagtag gcgtgtgcca cgtcgcggat acagcctttt tcgcggtcaa
    27661 ggtcgagggt tttccaaata cggttttgcg agaacgcctg agtggtgcgc acgccgccgg
    27721 gagccgcttt gaaacgttcg atggcgcggg tgttttcggg attggtgacg tcccattttt
    27781 caatcgcgtc tttcagtgtc ggcgcgtgga tcgtgtacac gtcggtgtgc agtttgcccg
    27841 ctttgtcaag ttctttcaga atggcgaaga tgccgccggc gcggtgcacg tcttccatat
    27901 agtagtcgtg gttgttgggc gcggttttgc agatgcaggg cacgacgcgg cttaagcggt
    27961 cgatgtctgc cattttgaaa tccacgcccg cttcgttggc gacggcgagc aagtgcagga
    28021 tggtattggt gctgcctccc atcgcaatat ccatcgtcat ggcgttttca aacgcttttt
    28081 tggtggcaat gctgcgcggc agcacggttt cgtcgtcttg ctcgtaatag cgtttggtga
    28141 tttcgacaat catacgccct gcttcgagga acaattcttt gcggcctgcg tgggtcgcca
    28201 aatacgaacc gttgccgggc agggacagac cgagcgcttc ggtcaggcag ttcatcgagt
    28261 ttgccgtaaa catacccgaa cacgagccgc aggtcgggca ggcgttttgt tcgacttctt
    28321 cgacttgccg gttgctgaca ttgtcgtccg ccgattcgat catggcgtca atcaagtcca
    28381 aacggcgttc gggttggatg tttgccacgc cgatgacctt gcccgcttcc atcgggccgc
    28441 cggagacgaa gatggtgggg atgttcaggc gcatggcggc aatcagcatt cccggggtga
    28501 ttttgtcgca gttggaaatg cacaccagcg cgtcggcgca gtgggcgttg accatatatt
    28561 cgatggagtc ggcaatcaaa tcgcggctgg gcagggagta caacataccg ctgtgcccca
    28621 tggcgatgcc gtcgtcgatg gcgatggtgt tgaattcttt ggcaattgca cccgcttttt
    28681 caatctcgcg ggcaaccagc tggcccatat tgtgcaggtg gacatggccg ggcacgaatt
    28741 gggtgaagga gttggcaacg gcgatgatgg gcttgccgaa gtcggtttcc atcacgccgg
    28801 tggcgcgcca caatgcgcgc gcgcccgcca tattgcggcc gtgggtggag gttttggagc
    28861 ggtattctgg catagtgtgt ttccttgtgc ctataccgtc tgaaaggcag ggctgtttca
    28921 gacggcatcg ggtacggttt tttagagtgg gaaaaggggg tattttatac caagtatcgg
    28981 aattttgcgg aatggaaacg tgtgcggcaa aaaagaacat ccccgcagga atgcggggga
    29041 cgggttcagg cgcgcgcaat cgggacggct ttggatgcgt cccaaaaatc gacgggcgca
    29101 tccaacacgg gtttgacgat gcccgtccgt atggcgaaat cgccgaaacc ttcgtcggta
    29161 tcgcgtcccg ccgcccattt gccgatcagg tcttccaatt cggaaaggat ttccggcagg
    29221 gtgatgtttt ctttgtagag gcgggggatg cgtacgcctt cgcggtcgcc gccgatgtgg
    29281 aggttgtagc gtcccacggc tttgccgacc agtccgattt ccgccaacat cgcccgtccg
    29341 cagccgttcg ggcagccggt aatgcgggta acgatgtagt cgtccgacgc gccgtgttcc
    29401 gccataatct tatccagctc gccgatgaag tccggcaaca cgcgttcggc ttccgccatt
    29461 gccagcgggc aggtcgggaa ggaaacgcac gacatcgcat tttcgcgcag cttgctgaca
    29521 ccgtcgcgta tcagcccgta tgagcgggcg agctgttcga tccgggcttt gcctccttcg
    29581 ggcacatttg ccacgatgag gttttggttg gcggtgatgc ggaagccgcc tttgtggatt
    29641 ttggcgattt ccaacacgcc ggtcagaagc tgtttcccgc cttcgtcaac caaacgcccg
    29701 ctttcgatga aaagggtcaa atgccagttg ccgtctatgc ctttcacccg gccgatgcgg
    29761 tcgccgcgcc cggtaaactt gaacggccgt atgggtttga acggcatacc catacggcgt
    29821 tcgacttccg tgcggaagtt gtccaagccc atattttgaa tggtgtagcg ggtgcggtcg
    29881 tttttgcggt ggctgcggtt gccgaagttg cgctgcgtgg ttaccaccgc ttcggcggcc
    29941 ttcagtgcgt gttccggagg cacgaaaccc agttccagtg aaatgttcgg ataggttttg
    30001 gtgttgccgt tgccgttgcc gtgttccgtc gaaagcccgc cgcccgccaa aacattgaag
    30061 ccggcaagct gtccgttgcc gtctgaaacg gcgacgaaat ccaaatcgtt accgtagcag
    30121 tccacatcgt tcaagggcgg gatgacgact gcggttttga attttcgcgg cagataggtt
    30181 ttgcccaaaa tcggctcgcc ttcttgaagg aagtcgtcgg aactttgaac ttttttgccg
    30241 tccacccaca tatccagata accgcgcgtg cgcggcagca ggtgttcgga aatctttttc
    30301 gcgtattcgt aagcctgccg gtgcagctcg gactcgatcg ggttggaggt gcaaagcaca
    30361 ttgcggttca tatccgccgc cgtagcgatg gaatccaaac ccaggctgtg caagaggcgg
    30421 ggcatcgtct gcaacttggt tttcggcacg ccgtgaaatt ggaaggtttg ccggttggtt
    30481 agccggatgg agcggtaatg actcttttcc cgggcgaatt tgtccagttc tatccactgg
    30541 gacggtttga tgatcccgcc ggcagccggc agcgcaaaag cataaatttc aagggctcga
    30601 gttttgcctc ggtgcggatg tcgcggccat cctgctcata catactgtgg aagcggatga
    30661 gttggaagtt gtcgcctttg aagccgcccg tgagcgggtc tttcaaatcg tccaaaatcg
    30721 tgccgcgtaa aaaattgctt tcggttttca ggcgttcgtt gtcggatagc ggcttttctt
    30781 gccacgccaa accttttgtc ttggcctgta cggtcatttt gtgttcctcc cgattatatt
    30841 taatcaataa acatcacgct gatagcgttt ttcttcgcgc agcatatcca aatatccttc
    30901 tgcgccgtct tcgtccgaat gccctgcccc gataatcaca tccagcaagg cggcttccac
    30961 ttcttttgcc atttttgccg catcgccgca cacatagata tgcgcgcctt cctgcagcca
    31021 ttgccaaagt ccttccgcct gttcgcggat tttgtcctgc acatagattt tttcttcctg
    31081 atcgcgcgac caggcgaagt catatctgtg caggaagccg tctttggcaa actgctgcca
    31141 ttcggtctga tagaggaagt cggcggcaaa atgcggattg ccgaaaatca gccagtttct
    31201 gccttccgca ttttctgcgg cacgttgttg gacgaaggcg cggaacggtg cgacgccggt
    31261 accggagccg atcatcacaa tcggcttgcg gctgtcttcg ggcagcctga agccgtcgtt
    31321 gcgttccgca aacacgcgca ccgtgccgtc ctcttccagc cggtcggcaa agaaacccga
    31381 tgcgccgccc gccctggcgc gcccttcgtg ttcgaaacgc actgcgccga cggtcaggtg
    31441 cacttcgtcc cccgcttccg cctgcgacga ggaaatcgaa tacaggcgcg gcgcaagcgg
    31501 gcgcagcagg ccggcgaatt gttccgccgt cagttttgcc gggaagcggt gcagcacacc
    31561 ggcaatcggc gtgctttgca caaagccttg caaaacggcg ttgtcggcag caatacggtc
    31621 gagttcgtca tcatcggcga acgtggcata gcctttgaca aaggcggggg tgttttgcgt
    31681 gagttcgaaa tgggataaca gtgcggaggc aaccggcagg gtttttccgc ccgcctgtat
    31741 ttccgttgcc ggatcgatgc cgagcaggtc taggatttcc ccgaccagtg ccggatcgtt
    31801 gtcaaaccaa acgccgagcg cgtcgcccgg gaggtagtgc aaatccgaac cgctcaaatc
    31861 gatttcgatg tggcgcacgt ctttatcgga ttggcgggcg gtgattttct gattggccag
    31921 cagggcggcg ggaaaggggt ctgccttgca gtacctgccc tccgatgccg tctgaaggcc
    31981 ggcgggggga cgttgcctgc ggcgcgggcg ttgcccggtt ttttgcggct tcttctttta
    32041 agcgcgcgac aatgcggcct gtccatccgt ctgcggcggc ggcaaagtcc aaatccgcat
    32101 caacgcgttc gagcaggcgt tttgcgccca attcttcaaa acgtttgtcg aaatctttgc
    32161 ccgcccggca gaaattcgga taggagctgt cgcccaaacc cagtacggca aattggagtt
    32221 tgtccaattt cggggctttt ttgccgttca gcagtttgtg cggcacgacg gcttcttccg
    32281 gcggttcgcc ttcgccctgg gtggacgtaa ccagcagcag gcggcgttcg ccggcgatgt
    32341 ttttcgcctt gtagcctttc agttcggcgc gcctgacttg gatgccggcg gctttcaggc
    32401 tgtccgccgc tttgtcggca acggatttcg cattgccggt ttgcgaggcg gaaaggacgg
    32461 ttacggaaaa aggttctgcc gccggcaatg ccgtctgaag cgcgggcagc cctgcggatg
    32521 ccccgtttcc tgcttttgcc caagcgtagc cggacagccc cgacacagga gctgcgtgat
    32581 ttcgggcggc ataggcggta atggcggatt tgtgttctgc atatcgtgtt cactcataaa
    32641 atcatacctg ccgcaacagt gccgtatgtc gcttcgtcta tcaggataaa cgaaccggcg
    32701 gcggtgtttt ccgcataagg cgttgccgta acgggttttt gaaggttgat gcggactttg
    32761 gcgatgtcgt tcatctttaa ggattccgcg ccggcctctt gttccagcgt gcggacatcc
    32821 aaaacgcttt caatttgccc gacttttgcc ggcacggttt gcgtgccgtg cttgagcagg
    32881 tatttgcgcg cggtgttgag cgggcgttcg tcaaaccagc aaagcgtggc ttccggatgt
    32941 ttttgcgggg cgagcgggga atttttatcg acaaaaagat cgccgcgcga aacatcgatg
    33001 tcgcggtcca gccggatggt tgccgcctcg ccggcaaaag cctgcgccac ttcccctttc
    33061 ggcacgatga tttcggacac ttcggcggtc agcccgttcg gttcgatgcg gacggtttgc
    33121 ccgacggtga ccgaaccgcg ttcgatgcgc ccctgatagc ctcggaaacc gtcggccttg
    33181 tcggcatctt ggcggacgac cagttggacg gggaaataaa aaccggcatc gcgccacctt
    33241 cctgaaattt ccgccatatc gcccaaagac ggcggcagca aggcttccag cctttcacgc
    33301 agcaggcggg cgaggacggg cgcgctgccg gagctggaaa cggcaatctg aacagggtcg
    33361 cggtcgataa ccgacgggaa gatgaagctg caatggtcac ggtcgcccac cacgttgacc
    33421 ggcttttggc agctttcggc aagatggaaa acgcgccggt tgagggcttg gtcgctgctt
    33481 gccgcaatga tgaggaaaac cgtgcggatg tgttcggcac gaaattcttt ggcaagccac
    33541 aggattttgt tttccgccgc caacgcggag agttcggcat tcaggtgttt tgcggcaacc
    33601 ccgacctctg cgcccgcctt cggcagcagg ctgattttgc gtgcggcgac cgcgccgccg
    33661 cctacgacca atacggggcg gccggagagg ttggcgaaaa tgggaaaata attcactggc
    33721 tgactccttt gctgtttgcc cgcaccttgt ttccgatacg gtgcgtcgcg gcatttttgt
    33781 cggaatgcgg gtcattttaa ataataaaaa ggcatttgtt agaagctgaa agctatatgg
    33841 ggggcggctg cggatgcggc ggttttccgt tttatagcgg attaacaaaa accggtacgg
    33901 cgttgccccg ccccggccca aagggaacgg ttccctaagg cgcccaaaca ccgggcgaac
    33961 cggttccgta ccattcgtac tgcctgcggc ccgccgcctt gtcctgattt ttgttaatcc
    34021 gctatataac ggttttggac ggaaaaacgg cctgaagccg tttcgggcat tcagaccgtt
    34081 tgtgcggtgg ggaaaatgcc gtcagaaagg cagaaaaggg cttcagacgg catgatgtcg
    34141 ggtttcagga caggagcagg atggcggctg cggcaagcgc ggcaaccgat aatgcggcgg
    34201 caagcgcggc tttgccggca aagcggattg aggttttgcc ttcgatgtat ttgaagccgg
    34261 ttatcatcgg gcggacgagg tttttctttt tgaatatgcg gtatgcggcg acggcggcga
    34321 tgtggactgc ggaaaaaacg gcgagcagct tgaaaaagtt gaggtggatt ttccgtataa
    34381 ggctgcccgt atgttcggaa accaaatggt tgaggtagcc gttggtgctg aaggtgtttt
    34441 catcggcggc aaaaagcccc gtgccgactt gaaatgagac ggcggccaaa agcgcaacga
    34501 ccatcagtgc gcccaagggg ttgtgtccgg gctggatatg ttcgggaatg ccgtttttca
    34561 gatagccgcg tatacctgcc caacctcgga cgaaacggga gaaacgggcg gtatcgctgc
    34621 cccaaatgcc ccagcagagg cggaatacga gcaggaaaag gacgagcagc ccgacgcgcg
    34681 tgtgccattg cagcatatcg ccgccggctt ttgcgctata ccacataaag ggcagggatg
    34741 cggcaagcag ccagtggaaa aggcgggtgg ggaagtccca gactttggtt ttgtttttca
    34801 taatcggttt ccggcggtag aatcggtttg ttttcgagcc ttaattttaa ccgattggag
    34861 gggaaatgtt tcccgttttc catctttcag gcgaaagccg ccgccggatg cttcagacgg
    34921 cattgcgttt tccccatgtt ttcaaagccc gtgcggaaga ttcgcacaaa gggactttcg
    34981 gcacgctcgc cgtagtcggc ggatcggcag ggatgagcgg cgcgcccgta ttggcggcat
    35041 cggcggcaat gtatctcggc tgcggtaagg tgcgggcagg tttcaatcag gatacgctgc
    35101 cttttgccgt cattgccggt tttcccgaga ttatgttgga cacggcagac ggtttgacca
    35161 agcgtcaagg cataaacgct tggacggcgg gttgcggctt gggtacagat gcggcggcag
    35221 tggaaacggt ggcggcagtg ctggcgcgga atcgagacga agccgtcgtt ttggatgcgg
    35281 acgcgctgaa catattatca accgatgccg aaacccgaaa tctggcgcgc gggtgtaaaa
    35341 acctgatttt aacgccgcac cccgccgaag ccgcgcgcct gcttggaacg acagttgcac
    35401 aggttcaggc ggatcggacg gcggcagtaa ggaagatagg ggcaattctc ggtgcaaccg
    35461 tggttttaaa ggggcacaaa acattggttg ccgcgtccga tacggaaatc tatgtcaacg
    35521 aaagcggcaa tgcgggattg gcaacggcgg gcagtggcga cgtattgggc ggcatcatcg
    35581 gcagtctgct cgcacagggc gtgccggttt ttgaagccgc ctgcgcgggc gcgtggctgc
    35641 acggcgcggc ggcggatgtt ataaaggaat cggcaggcat tgcggctggg ctgtcggcag
    35701 gggaaatcgc cccggcggca aggtggctgc gcaactggat aactgaaagt atgtaagaag
    35761 atgccgtctg aaaggcaagg gcttcagacg gcatcttcat tttccaaata ctgtccggta
    35821 aatcgtggta taatacgcag ttatctctta cagtttttga aaaacattaa ttatgaaaca
    35881 aatccgcaac atcgccatca tcgcacacgt cgaccacggc aaaaccacat tggtcgacca
    35941 actgctgcgc caatccggca cattccgcgc caaccagcag gttgacgagc gcgtgatgga
    36001 cagcaacgac cttgaaaaag aacgcggcat caccatcctc gccaaaaaca ccgccatcga
    36061 ttacgaaggc tgccacatca atatcgtcga cacgccggga cacgccgact tcggcggcga
    36121 agtggagcgc gttttgggga tggtggattg cgtcgtcttg ttggtggacg cacaggaagg
    36181 tccgatgccg caaacccgtt tcgtgaccaa aaaagccttg gctttggggc tgaaaccgat
    36241 tgtcgtcatc aacaaaatcg acaaaccgtc cgcccgtccg agctgggtta tcgaccagac
    36301 tttcgagttg ttcgacaact tgggtgcgac cgacgagcag ttggatttcc cgattgttta
    36361 cgcttcaggt ttgagcggct ttgccaagct ggaagaaacc gacgagagca gcgatatgcg
    36421 cccgctgttc gacaccatcc taaaatacac gcctgcaccg agcggcagcg cggacgagcc
    36481 gctgcaactg caaatttccc aactcgacta cgacaactac accggccgcc tcggtatcgg
    36541 tcgtatcttg aacggacgca tcaaacccgg ccaaaccgtt gccgtgatga accacgagca
    36601 gcaaatcgcc caaggccgca tcaaccagct tttgggtttc aaaggcttgg aacgcgtgcc
    36661 gcttgaagaa gccgaagccg gcgacatcgt gattatttcc ggtatcgaag acatcggcat
    36721 cggcgtaacc atcaccgaca aagacaaccc caaaggcctg ccgatgttga gcgtggacga
    36781 accgacgctg acgatggact ttatggtaaa caccagcccg ctcgcaggta cagaaggcaa
    36841 attcgtgacc agccgccaaa tccgcgaccg cctgcaaaaa gaattgctga ccaacgttgc
    36901 cctgcgcgtg gaagacaccg ccgatgccga cgtgttccgc gtatccgggc gcggcgaact
    36961 gcacctgacg attttgctgg aaaatatgcg ccgcgaaggc tacgaactcg ccgtcggcaa
    37021 gccgcgcgtc gtgtaccgag acatcgacgg tcaaaaatgc gaaccttatg aaaacctgac
    37081 tgtggacgta cccgacgaca accaaggcgc ggtaatggaa gaactcggcc gccgccgtgg
    37141 cgaactgacc aatatggaaa gcgacggcaa cggacgcacc cgcctcgaat accatattcc
    37201 agcgcgcggc ttgatcggtt tccaaggcga attcatgacc ctgacgcgcg gcgtcgggct
    37261 gatgagccac gtgttcgacg actacgcgcc cgtcaaaccc gatatgcccg gccgccacaa
    37321 cggcgtactg gtgtcccaag agcagggcga ggcggttgct tacgccttgt ggaatcttga
    37381 agaccgcggc cgtatgttcg tatcgcccaa cgacaaaatc tacgaaggta tgattatcgg
    37441 catccacagc cgcgacaacg atttggtggt caacccgctc aaaggcaaaa aactcaccaa
    37501 tatccgtgcc agcggtaccg acgaagcggt gcgcctgacc acgccgatca aactgacgct
    37561 ggaaggcgcg gtcgagttta tcgacgatga cgagctggtg gaaatcacgc cgcaatccat
    37621 ccgcctgcgc aagcgttacc tgagcgaatt ggaacgccgc cgtcatttta aaaagctgga
    37681 ttaatgttta aatgatacgc gatgccgtct gaaaaatttc agacggtatt ttttattcgg
    37741 acgggctttg cggcttcttg aagctgtttc agacggagtt tttcctatcc aatcaagaaa
    37801 ctgccgccat ttttccagcg gcatatcgcc ccgtcgcgtt tcggtatcgg gttcggtttc
    37861 ccggcagcat gtcgaacaat attgtttgaa gggatacgcc ttttgagtcc ttacaggctt
    37921 aggaagagga taaaacatac cgcaaaacat accataaaaa aacggtaacg gatagtaatc
    37981 aatggtaacg tagggtaata ggttgtagct gattttacgc aataaaatca atacgtaagt
    38041 aacaaaaaat cccaaactgt aaaagtttgg gatttgtatt tggcagagag gaagggattc
    38101 gaaccctcga tacgctattc acgtatacac gctttccagg cgtgcgactt aaaccactca
    38161 tccacctctc taatggcgga aattatccta atcgggataa tttgttattt ggtgcccggg
    38221 agaagactcg aacttccata cccgtgagga taccagcacc tgaagctggc gcgtctacca
    38281 attccgccac ccgggctttg ctttatttgc tgttttgcgg tacagtgtcc tgccgcaaag
    38341 aagtcgctat tatatagttc ataaagagaa tgtcaacagt caaaatgaat aaaaatatta
    38401 aatctttaaa tttacgggaa aaagacccgt ttttaagtcg tgaaaaacag cgttatgaac
    38461 atcctttgcc cagtcgggaa tggataatcg aattgttgga gcgcaaaggt gtgccttcaa
    38521 aaatcgaatc gcttgcacgc gagctgtcga ttacggaaga cgagtatgtc ttttttgaac
    38581 gccgtctgaa ggcgatggcg cgggacggtc aggttttaat caaccgccga ggcgcagttt
    38641 gcgcggcgga caagctggat ttggtcaaat gccgcgtcga ggcgcataag gacggtttcg
    38701 gcttcgccgt gccgctcatg ccgatggacg aaggggattt cgttttatac gaacgccaga
    38761 tgcgtggtgt catgcacggc gacaccgtta ccgtccgtcc tgcgggtatc gaccgcaggg
    38821 gccgccgcga agggacggtt ctggatattg tcgaacgcgc gcaaagcaaa gttgtcggcc
    38881 gtttttatat ggataggggc gtggcgattt tggagccgga agacaagcgt ctgaaccaaa
    38941 gcatcgtgtt ggaagcggac ggcgtggcgc gtttcaaacc cgaatccggt caggttatcg
    39001 tcggcaaaat tgaggtttat cccgagcaaa accggcctgc agtggcaaaa atcattgaag
    39061 ttttgggtga ttatgccgac agcgggatgg aaatcgaaat tgccgtgcgc aagcatcatt
    39121 tgccgcaccg attcagtgaa gcgtgtgcca aagccgcgaa aaaaattccc gaccatgttc
    39181 gtaaaagcga tttgaaaggc cgcgccgatt tgtgcgacct tcctttggta acgatagacg
    39241 gcgaaacggc gcgtgatttc gacgatgcgg tgtttgccga gaaaatcggg cgcaattacc
    39301 gtttggtcgt ggcgattgcc gatgtcagcc attatgtcgg cccagatgat gcgattgata
    39361 cggacgcgca ggaacgcagc accagcgttt atttcccgcg ccgtgtaatt ccgatgctgc
    39421 cggaaaactt atccaacggc atctgctcgc tcaatccgga tgtcgagcgt ttgtgtatgg
    39481 tgtgcgatat gaccgtcact tacgcgggca atgtcaagga atatcaattt tatcccgccg
    39541 tgatgcgctc ccacgcaagg ctgacttaca accaagtttg ggaatggctt tcagacggta
    39601 tcgggcatcc gttcaaaacc caaatcgaca cgctttacaa gctgtttaaa attttgcaga
    39661 aaaaacgtct ggcgcgcggg gcggtggagt ttgaaagcgt cgaaacccag atgattttcg
    39721 acgacaacgg caaaatcgaa aaaatcgtgc ccgtcgtccg caacgatgcc cacaagctga
    39781 ttgaagaatg tatgctggcg gcgaatgttt gcgcggcgga ttttctgttg aaaaacaaac
    39841 atacggcttt gttccgcaac catttgggcc ccacgcccga aaaactcgcc accctgcgcg
    39901 agcagctcgg tctgttgggg cttcaacttg gcggcggcga caacccgtcg ccgaaagact
    39961 atgccgcgct tgccgaacaa ttcaaaggca ggccggatgc cgaattgctg caagtcatga
    40021 tgttgcgctc catgcagcag gcggtttacg aaccgcattg cgaagggcat ttcggtttgg
    40081 cttatgaagc atacgcccac tttacctcgc ccatccgccg ctatcccgac ctgaccgtcc
    40141 accgtgccat caaagccgta ttgaaccgga aaacctacac gccaaacaaa agctggcagg
    40201 ctttgggcgt gcatacttcg ttttgcgaac gccgtgccga cgatgctggc cgcgatgtgg
    40261 aaaactggct gaaaacttat tatatgcgcg ataaggtcgg tgaaatattt gaaggcaaaa
    40321 tctccggggt ggcaaatttt ggaatatttg tcactttgga cgatatccat atcgacggtc
    40381 tggtacatat cagcgatttg ggcgaagatt atttcaactt ccgccccgaa atcatggcaa
    40441 tcgaaggcga acgcagcggc atccgtttca atatggggga cagggttgcc gtccgggtcg
    40501 cgcgtgccga tttggatgat ggaaaaatcg actttgtcct aattgccgga gaaagcggca
    40561 ggcggcggaa ggtcaaatta tccgcatctg ccaaaccggc aggggcggcg gggaaaggga
    40621 aatcgaaaac caccgccgag aaaaaaacag cccgatgcgg caaagtaagg ggaaggggcg
    40681 tgcctgccgt tgccgaatcg gggaaaaagg caaagaaacc ggttccgatt aaggtcaaaa
    40741 aacggaaagg
//
LOCUS       DAXFZR010000009        38515 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000009 DAXFZR010000000
VERSION     DAXFZR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38515
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.009"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..260
                     /locus_tag="ACFJAS_000355"
     CDS             <1..260
                     /locus_tag="ACFJAS_000355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981702.1"
                     /translation="LLSLICSVLSSYFEGNISANLVQTLVFYSPVSVFCHSRFRFQQT
                     AFKQLKEKGQIAQGSPFNQIRISIPGNFGHLTVSAFLPRRQ"
     gene            complement(342..1127)
                     /locus_tag="ACFJAS_000356"
     CDS             complement(342..1127)
                     /locus_tag="ACFJAS_000356"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC3981703.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            1371..2186
                     /locus_tag="ACFJAS_000357"
     CDS             1371..2186
                     /locus_tag="ACFJAS_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC3981704.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2227..3096
                     /locus_tag="ACFJAS_000358"
     CDS             2227..3096
                     /locus_tag="ACFJAS_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC3981705.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            3132..4388
                     /locus_tag="ACFJAS_000359"
     CDS             3132..4388
                     /locus_tag="ACFJAS_000359"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC3981706.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(4446..4586)
                     /locus_tag="ACFJAS_000360"
     CDS             complement(4446..4586)
                     /locus_tag="ACFJAS_000360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981707.1"
                     /translation="MEYGYATVYFGKEKENCRQARIKSDEARDSAFGVESVESAVFDI
                     FN"
     gene            4734..5162
                     /locus_tag="ACFJAS_000361"
     CDS             4734..5162
                     /locus_tag="ACFJAS_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3981708.1"
                     /translation="MNFALSVITFTLASFLPVPPAGTAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(5211..6554)
                     /locus_tag="ACFJAS_000362"
     CDS             complement(5211..6554)
                     /locus_tag="ACFJAS_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC3981709.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(6559..7212)
                     /locus_tag="ACFJAS_000363"
     CDS             complement(6559..7212)
                     /locus_tag="ACFJAS_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC3981710.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            7362..7652
                     /gene="gatC"
                     /locus_tag="ACFJAS_000364"
     CDS             7362..7652
                     /gene="gatC"
                     /locus_tag="ACFJAS_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC3981711.1"
                     /translation="MALTLADVDKIARLSRLQLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEVALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            7715..9160
                     /gene="gatA"
                     /locus_tag="ACFJAS_000365"
     CDS             7715..9160
                     /gene="gatA"
                     /locus_tag="ACFJAS_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC3981712.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSFEREKEDYTRDLDKPLKGVKIGLPKEYFSEGNSTDVQTA
                     LQNTIDLLKAQGAEPVEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADTSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            9157..10089
                     /locus_tag="ACFJAS_000366"
     CDS             9157..10089
                     /locus_tag="ACFJAS_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC3981713.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDVLC
                     QMEDCRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDDMVAKMKDSLKTEARGISDGIQTEKPLRRTV"
     gene            10132..11562
                     /gene="gatB"
                     /locus_tag="ACFJAS_000367"
     CDS             10132..11562
                     /gene="gatB"
                     /locus_tag="ACFJAS_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC3981714.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGGDVKTINVTRAHMEEDAGKSVHEGLNGTTGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRIDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEALANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            11654..12663
                     /locus_tag="ACFJAS_000368"
                     /pseudo
     CDS             11654..12663
                     /locus_tag="ACFJAS_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
     gene            complement(12793..13425)
                     /gene="pdxH"
                     /locus_tag="ACFJAS_000369"
     CDS             complement(12793..13425)
                     /gene="pdxH"
                     /locus_tag="ACFJAS_000369"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC3981715.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            13485..13637
                     /locus_tag="ACFJAS_000370"
     CDS             13485..13637
                     /locus_tag="ACFJAS_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981716.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            13646..14695
                     /locus_tag="ACFJAS_000371"
     CDS             13646..14695
                     /locus_tag="ACFJAS_000371"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC3981717.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(14754..16295)
                     /locus_tag="ACFJAS_000372"
     CDS             complement(14754..16295)
                     /locus_tag="ACFJAS_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC3981718.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYVGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQSAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(16797..18152)
                     /gene="xseA"
                     /locus_tag="ACFJAS_000373"
     CDS             complement(16797..18152)
                     /gene="xseA"
                     /locus_tag="ACFJAS_000373"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC3981719.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
     gene            18349..19170
                     /gene="nadE"
                     /locus_tag="ACFJAS_000374"
     CDS             18349..19170
                     /gene="nadE"
                     /locus_tag="ACFJAS_000374"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC3981720.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(19241..19714)
                     /gene="dnrN"
                     /locus_tag="ACFJAS_000375"
     CDS             complement(19241..19714)
                     /gene="dnrN"
                     /locus_tag="ACFJAS_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC3981721.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            20012..20344
                     /gene="trxA"
                     /locus_tag="ACFJAS_000376"
     CDS             20012..20344
                     /gene="trxA"
                     /locus_tag="ACFJAS_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC3981722.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(20396..21343)
                     /locus_tag="ACFJAS_000377"
     CDS             complement(20396..21343)
                     /locus_tag="ACFJAS_000377"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3981723.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            21671..23059
                     /locus_tag="ACFJAS_000378"
     CDS             21671..23059
                     /locus_tag="ACFJAS_000378"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3981724.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSASGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            complement(23173..23279)
                     /locus_tag="ACFJAS_000379"
                     /pseudo
     CDS             complement(23173..23279)
                     /locus_tag="ACFJAS_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(23410..23610)
                     /locus_tag="ACFJAS_000380"
     CDS             complement(23410..23610)
                     /locus_tag="ACFJAS_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981725.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            complement(23624..24178)
                     /locus_tag="ACFJAS_000381"
     CDS             complement(23624..24178)
                     /locus_tag="ACFJAS_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981726.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            complement(24263..24764)
                     /locus_tag="ACFJAS_000382"
                     /pseudo
     CDS             complement(24263..24764)
                     /locus_tag="ACFJAS_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25168..26361
                     /locus_tag="ACFJAS_000383"
     CDS             25168..26361
                     /locus_tag="ACFJAS_000383"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC3981727.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(26468..27712)
                     /gene="clpX"
                     /locus_tag="ACFJAS_000384"
     CDS             complement(26468..27712)
                     /gene="clpX"
                     /locus_tag="ACFJAS_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC3981728.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKARHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            27895..28266
                     /gene="rbfA"
                     /locus_tag="ACFJAS_000385"
     CDS             27895..28266
                     /gene="rbfA"
                     /locus_tag="ACFJAS_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC3981729.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            28277..28801
                     /locus_tag="ACFJAS_000386"
     CDS             28277..28801
                     /locus_tag="ACFJAS_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981730.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            28859..29779
                     /gene="truB"
                     /locus_tag="ACFJAS_000387"
     CDS             28859..29779
                     /gene="truB"
                     /locus_tag="ACFJAS_000387"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC3981731.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDNLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASAA"
     gene            29996..30520
                     /locus_tag="ACFJAS_000388"
     CDS             29996..30520
                     /locus_tag="ACFJAS_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC3981732.1"
                     /translation="MKTGIQTELAQALLPHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPASQPASQPASQPASQP
                     IIHQINQKKTRNLF"
     gene            30405..32198
                     /locus_tag="ACFJAS_000389"
     CDS             30405..32198
                     /locus_tag="ACFJAS_000389"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC3981733.1"
                     /translation="MASQPASQPASQPASQPASQPASQLYTKLTRKRQEIFFNQTLAF
                     DEIDRLFDAKAFSKFSRHTADGKQPVGEIKRRSDGTPAENLIIKGNNLIALHSLAKQF
                     KGKVKLIYIDPPYYFVKKKPQDSFGYNTNFKLSTWLTFMKNRLLIAKELLTDDGIIVI
                     SIDDDGNAYLKILLDEIFGFENFIGNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDK
                     STFYEFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVS
                     TITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFILPIADKQWGRWRWGYSIKNK
                     ARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGE
                     KAFKNPKPEELIQDFITITTNENDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIET
                     LAVERMKKVIDGEQGGISKAVNWQGGGEFVYAELSPFNETAKQQILACENSDGIKTLF
                     EGLCERHFLKYNVSVNEFSQIIEEPEFQSLALDEQKQMMLEMLDLNQMYISLSEMDDE
                     QFAGCLNDDDKALSRAFYQSVKHQAEKKDGE"
     gene            32188..34962
                     /locus_tag="ACFJAS_000390"
     CDS             32188..34962
                     /locus_tag="ACFJAS_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC3981734.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVILGDEAHHLNAQTKGKKQGELDLEKEMNE
                     RTSDAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENAEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWIKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(35111..36283)
                     /locus_tag="ACFJAS_000391"
     CDS             complement(35111..36283)
                     /locus_tag="ACFJAS_000391"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC3981735.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(36404..36646)
                     /locus_tag="ACFJAS_000392"
     CDS             complement(36404..36646)
                     /locus_tag="ACFJAS_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981736.1"
                     /translation="MVSLMVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSL
                     ILLYLFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            36633..37079
                     /gene="iscR"
                     /locus_tag="ACFJAS_000393"
     CDS             36633..37079
                     /gene="iscR"
                     /locus_tag="ACFJAS_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC3981737.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            37108..38322
                     /locus_tag="ACFJAS_000394"
     CDS             37108..38322
                     /locus_tag="ACFJAS_000394"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC3981738.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
BASE COUNT        10080 a        10561 c         9399 g         8475 t
ORIGIN      
        1 tacttctttc tttaatttgc agtgtgttat cttcatattt cgagggtaac atatctgcta
       61 atctggtaca gacccttgtt ttttattccc cggtgtctgt tttctgccac agccgattta
      121 gattccagca aaccgccttt aaacaattga aagaaaaggg acaaattgca cagggttctc
      181 cgtttaacca gatacggatt tccattcccg gcaatttcgg gcatttgacc gtttccgcct
      241 tcctgccccg ccgccaataa aaatgccgtc tgaaatatcg gcacgatact tcagacggca
      301 tacccgcccg ttttcccgca tttccgggag ctggctgaaa tttaaacgtg tgcggaaatg
      361 atttttaaca tttgcgccag tactttggga ttcgcagcca caatatcgcc gctttccaac
      421 caagcgtcct cacccgacat atcggtaacg atgccgcctg cttcttggac aatcaatgca
      481 ccggcggcaa tgtcccacgg tttgaggttg aactcgaaaa agccgtcaaa acgtcctgtt
      541 gctacggcgc acaaatccaa agaagccgcg ccttcgcggc ggcctccggc ggtttttgcc
      601 aaaaagtctt tcaaaatcgc cagatatttg tccatcatgc tttgatcgac aacagggaag
      661 ccggtaccga tcaggcagcg gttcaattcg atgcggttgg agacacggat gcggcggtcg
      721 ttaagcaacg cgcctttacc gcgcgaagcc atgtacacgt cgttacgttc gggggcgtac
      781 accaaagctt cctgcaacac gcctttgtgc agcagtgcca tagagatggc gtattgggga
      841 tggccgtgaa ggaaattggt cgtcccgtcg agcggatcga taatccattc gtactcggct
      901 gtagctttgc cgtggaagcc gctttcttcg caagtgattt tgtgatgcgg ataagcttct
      961 ttcaaagcct caaccaggat gatttcggaa ttgcggtcaa catcggaaac aaaatcgttg
     1021 aaggctttgc tgtcggtttt gacggcatcg aggttacctg cggcgcgaat catcatctga
     1081 ccggcacggc gggcggcttt aaaggctgta ttcaaaaacg gattcatcag atttccttaa
     1141 gggtggcata ccgccggttc ggacgtgcag tccttcggag cggcaaaatc ggagtttatc
     1201 tggttggggc aaaccctgcc aaatcgggta aaataccgcc tgacgcgtgt ctgcttcagg
     1261 cgcaacgtta aatttccgac gttgttaaag aacagttcag acggcatttc accgtccgaa
     1321 cgaaaaagac ggcgcattat accctattcc attccgaccg aaaaccgaac atgactgctc
     1381 tcaaacccgc cctgcccgac tacctcggca acatccgcat catcctcaca cgcaccagcc
     1441 accccgccaa catcggctct gccgcacgcg cgatgaaaac gatgggtctg cacaggctga
     1501 ccatcgtcac ccccaatctg atggcaacgc cgatgacgga aaatccgccc gtttttaatc
     1561 ccgatgacgt gcaaagtttt gcactgcctg aagaaagttt catcctcgct tccggcgcgg
     1621 cggacgtatt gcacaacgcc gaaatcgtcg ccacactgga cgaagccctt gccgacacca
     1681 ccatcgcctg cgccctgacc agccgccgcc gcgaaatcac cgcgccgctg caaaccccgc
     1741 gcgatttggt gcccgaatta ctgcaggccg ccaaccgcgg cgagaaagtg gcgctggtct
     1801 tcggcaacga gactttcggc ttgagcatcg aagaagtccg agcgtgcaac cgactgatga
     1861 ccatcaacgg caaccccgac tatttctcgc tcaacctcgc ccaagccgtg caggtcgtgt
     1921 gctacgaaat tttcagccaa accgattcgc ccatgaccca tcttcaacag gaagaccacg
     1981 ccgcaaccca cgagcaaatc aaaggcatgc tcgcccacat ggaaagcgtg atggacgaca
     2041 tcggcttttt caaccgccgc aacggcgagc gtctgatgcg ccgtatgcag agcctgttcg
     2101 gacgcgccaa cacgcaaacc gaagacatcg acatcctgcg cggttttttc aataccgtca
     2161 gccaccgtat ccataaaaaa gactgattaa ggccgtctga aaacattaca agctttttca
     2221 gacggcatga ctgatattcg gataagcatg aattacgccc tagacgcatt atggtggaaa
     2281 ctcaccagcc aacccgtccg cgacctcgcc tcgctgctga ctgcgccgcc tttgtggcaa
     2341 agcggttgcg aattgagcgt gcgcgaacta ctgggggaac gcggtttccg ctacctttta
     2401 gcattggatg ccgatcccgc gccgctgacg gattaccttg cacaacgcgc cccgttcgac
     2461 caccgtctcg gcatttatgc cgaagagctg ctggcttttt ggtttaccaa cgcaccgcac
     2521 accaaactgc acgcatataa tctgaccgtt ttttcagacg gccaaacttt aggcgccgcg
     2581 gattttgtcg tttccctcaa ccaacagccc tatcatatcg agctggcgtg taaatactac
     2641 ggcggagacc aagtgcagaa cctgcgcggt ctcaatccca aagacacgct gacggacaaa
     2701 gccgccaaat tggtgcagca attccagctg gtgcatacgt cgcaaggcaa agcaacctta
     2761 gccgcacaag acctgccgga aaatccactt cctgcttcca tcgtgcgcgg catcggattt
     2821 tttccacaag gtttccatgc ttttgagccg ccgcttaatc catacggctg gcgcggcatc
     2881 tatattcaag attgggcgga atacgggttt gaacgccaag aagcgcgcta tcacctgctc
     2941 gaccgcatgg tctatctcgc gcctgcgcgt gtcgccgaaa ccgaaacatt aaacgcaacc
     3001 gaaatccgcc gcatcgacca aggcttgatt gccgttttgg aatgtcggcc ggacggcttt
     3061 tggcacgaaa tcgaacgtat tatgaaggcc gtctgaaacc ctttaccaac tttaagagaa
     3121 cccattacat tatgaacgcc gcacaactcg accataccgc caaagttttg gctgaaatgc
     3181 tgactttcaa acagcctgcc gatgccgtcc tctccgccta tttccgcaaa cacaaaaagc
     3241 tcggccgcca agaccgccac gaaatcgccg aaaccgcctt tgccgcgttg cgacactatc
     3301 aaaaaatcag taccgccctg cgccgtccgc acgcgcagcc gcgcaaagcc gcactcgccg
     3361 cgctggttct cggcagaagt accaacatca gccaaatcaa agacctgctt gatgaagaag
     3421 aaacagagtt tttcggcaat ttgaaagccc gtaaaaccga gttttcagac ggcctgaata
     3481 ccgccgcaga attgccgcaa tggctggtgg aacaactgca acagcattgg agcgaagaag
     3541 aaatcctcgc tttcggccgc agcatcaacc aagctgcgcc gctcgacatc cgcgtcaata
     3601 ccttgaaagg caaacgcgac aaagtattgc cattgttgca agccgaaagc cccgatgcag
     3661 aggcaacgcc ttattctcct tggggcatcc gcctgaaaaa caaaatcgcg cttaacaaac
     3721 acgaactgtt tttggacggc acgctggaag tccaaggcga aggcagccag ctgcttgcct
     3781 tattggtggg cgcaaaacgc ggcgaaatca ttgtggattt ctgtgccgga gccggcggta
     3841 aaaccttggc tgtcggcgcc caaatggcca acaaaggcag aatctacgcc ttcgacatcg
     3901 ccgaaaaacg ccttgccaac ctcaaaccgc gcatgacccg tgccggactg accaacatcc
     3961 accccgaacg tatcggcagc gaacacgata cccgtatcgc acgactggca ggcaaagccg
     4021 accgcgtgtt ggtcgatgcg ccctgctccg gcttaggcac actgcgccgc aatcccgacc
     4081 tcaaataccg ccaatccgcc gagaccgtcg ccaaactttt ggaacagcaa cacagtatcc
     4141 tcgatgccgc ctccaaactg gtgaaaccgc aaggctgttt ggtttacgcc acctgcagcg
     4201 tgttgccgga agaaaacgaa ctgcaaatca aacgtttcct gtccgaacat cccgaatttg
     4261 aacccgtcaa ctgcgccgaa ctgctgcaaa acctgaaggt cgatttggat accggcaaat
     4321 acctgcgcct cgattccgcc cgacaccaaa ccgacggctt cttcgccgcc gtattgcaac
     4381 gcaaataaac cggtttgaac aaaatgccgt cagaaccctt ttcaaagcgt tcggacggca
     4441 tttcatcaat taaaaatatc aaaaaccgca gattcaaccg attcaacacc aaaggcagaa
     4501 tcacgcgcct catcggattt tatacgcgcc tgcctgcaat tttctttttc tttgccaaaa
     4561 tatacggtag cataaccata ttccaatgat atttttggga atctgtttta ccccaatata
     4621 taaagcacca tattaaggcg gagtgtcttc cctgccttga cccgaacccg gaaaagacac
     4681 cgcccgaacc aatcccgatt ctgcccgggc agtcagccat caaggaaacc ctaatgaact
     4741 ttgctttatc cgtcatcaca tttaccctcg cctctttcct gcccgtcccg cctgccggaa
     4801 ccgccgtctt tacttggaaa gacggcggcg gcaacagcta ttcggatgtg ccgaaacagc
     4861 ttcatcccga ccagagccaa atcctcaacc tgcggacgct ccaaaccaaa ccggcggtca
     4921 agcccaaacc tgccgtcgat acgaatgcgg acagtgcgaa ggaaaacgaa aaggatatcg
     4981 ccgagaaaaa cgggcagctt gaggaagaaa agaaaaaaat tgccgaaacc gaacggcaga
     5041 acaaagaaga aaactgccgg atttcaaaaa tgaacctgaa ggcggtggga aactcaaatg
     5101 cgaaaaacaa ggatgatttg atccgtaaat acaataacgc cgtaaacaaa tactgccgtt
     5161 aatcggctct agcgcaaacc cgatgccgcc tgaagcggca cggggtttgt ttatttctgc
     5221 cagtaggttt tgacgttgac gaactcgtac aacccgaatt cggacagttc gcgcccgtaa
     5281 ccggaatctt tgactccgcc gaaaggcagg cgcaaatcgc tgctggtatg gcggttgata
     5341 aacaccgatc cggcctgtat tttttcggca aaccgccagg cgcgttcagt atcggcggta
     5401 taaatgcagg caccgagccc gaacggggaa tcattggcaa ggcagatggc gtgttcttcg
     5461 ttttcggcgc gcaaaatcag ggcgacgggg ccgaatactt cttctctcca gacgcggcag
     5521 gcaggattta ccctgtctaa aaccgtcgca gggtaaaacc accccgaccc ctgtggaact
     5581 ttgcctccgg tcaggcacac cgcgccgttt gaaacggcat cttcaacctg cccgtgaacc
     5641 cggtcccgca aatcttcgcg gtgcagcggt gcaagcgtgg tatcgggatg tttcgggtcg
     5701 cccattttca atttggcgca ttcggcgaga aacagctgaa taaagcggtc ggctgcggct
     5761 tcggttacga tgatgcgctt ggcggcgtta cacgattgcc ccgcatcgcg gaaacgggaa
     5821 taacaggctt ctgcggcggc gcgttccaaa tcggcatcgg gcatcacgat aaaggcgttg
     5881 ctgccgccga gctccaacac ggttttcttg aggtttgcgc ccgcatgtgc cgcaagaatg
     5941 cgccccgtat gcgtcgaacc ggtaaacgcc atcgcatcgg tatcttcgac tgccttgagc
     6001 gtgtccgcct catccagcca cgcgcctgcc agaggaatac cgtctgaagc caaatcgaac
     6061 aacgcccgac tgacgcgtgc cacgctgggc gcgggtttga cggcgcacgc gttgcccgcg
     6121 cacatagcgg gaacggcaaa acgcaatacc tgccagacgg gatagttcca aggcatgacg
     6181 gcaaacacca cgcccaaagg ctcgaagcgc acctgactca aactcgcctg cgtcgcgatg
     6241 gttttgtggg caagcagttc gggggcaagg cgggcgtaat agcgtatcag ctcgatagac
     6301 ttgacgattt cggcacggca ttcgtgcagg cagcgtccga cttcttcgca caccatttcc
     6361 gcaaaacgct ctttttccgc ctccaaacgg ccggcaaatt tttgcagacg cgcggcacgt
     6421 tcggttacgc ccaattgcgc gaacgccctg ccgtgcattt tcaaatccgc cagccgccgt
     6481 tcaaactccg cataatcttg atcggggcgg cggtaaagcg tttcgcccgt aaatacattg
     6541 acactgtgaa acatcgaatc aacctgccag ttgcgggaat atcgttttca gtcccgacac
     6601 aataatctcc accgataccg ccgccagcat catacccata atgcggttta aaatcgtcag
     6661 ccccgtcgcg cccagcaggc ggctgacctt cccggcaacg attaaaatgg cataacaaat
     6721 cgcactgacc accaaaccgg ccgcgataat cagcgcgata tcgctgtacg ttttggctgc
     6781 cgaagcataa ataatcacag tcgaaatacc gcccggaccg atggtgatcg gtatggcgat
     6841 gggcacgacg gcaatcgccc ctgcattgcg ggcggggcgc gcttgccccg tttccggctg
     6901 cgcgccgaga ttctgcttgg cgggattgtc gttgccgttc atcatcgaaa tggcgatcag
     6961 cagcaccaaa atcccgccgc cgacctgaaa cgaaccgacg ctgatgccca aaaccttcaa
     7021 tagcgcaccg ccgatcagcg caaataccgc aatcacggca aacacggcga cggcggccgt
     7081 ccgcgcgacc ttcctgcgct ccttcgtgct gtgtccgttg gtcaggtcaa ggtaaagcga
     7141 caacgcgcta aacggattga tcagcaccaa aaaagccaca atcagcttgc cgatttccat
     7201 acccaatccc attatttccc ctctttcaaa cccgtgcggc aggcatccga tgctgcaaat
     7261 tgccgccgca acggattttt ccgttataat taaaaatcca agcaatacgc cccatcatac
     7321 ccgaacgacg gtatctttac catcagacaa ggatgctttt catggcactg acacttgccg
     7381 acgtagacaa aatcgcccga ctctcccgac tgcagctgac tgcggaagaa aaagaaaaat
     7441 cgcttcaaga attaaacgac attttcacta tggtcgaaca gatgcagaac atcaacacag
     7501 acggcatcga gccgatggcg cacccgcacg aggtcgccct ccgcctgcgt gaagacgaag
     7561 taaccgaaac cgaccgcgcc gccgaatacc aagccgttgc gccggaagta cgcaaccgcc
     7621 tgtacatcgt accgcaagtt atcgaagaat aatccgaata cgcttcagac ggcatcagca
     7681 ataccgcccg aagcccttta aggatggaag atttatgacc caatacacat tgaaacaggc
     7741 aggcagcctg ttgcagtcca aacagatttc cgccgtcgaa ctggcaagcg cataccttgc
     7801 cgccatcgct gaaaaaaatc ccgccctcaa cggctatatc accatcgacc aagataaaac
     7861 ccttgcagaa gcccgtgccg ccgacgaacg catcgcgcag ggcaacgctt cggcacttac
     7921 cggcgttccc gtcgcctaca aagacatttt ctgccaaacc ggctggcgca gcgcgtgcgc
     7981 ttccaaaatg ctcgacaact tcatctcccc ctacacggcc accgtcgtcc aaaacctgct
     8041 cgacgaaggt atggtaacgc tcggccgcac caatatggac gagttcgcta tgggttcgac
     8101 caatgaaaac tcattctacg gcgcggccaa aaacccgtgg aatccggaac acgtacccgg
     8161 cggttcgtca ggcggttccg ccgccgtcgt tgccgcgcgc ctcgcccctg ccgcgctcgg
     8221 ttcggacacg ggcggctcta tccgccaacc cgcatcgcac tgcggcatta ccggcatcaa
     8281 acctacctac ggcacggttt cccgcttcgg tatggtcgcc tacgcctcca gcttcgatca
     8341 agccggcccg atggcgcaaa ccgccgaaga ctgcgcgatt ctgttgaatg caatggcagg
     8401 tttcgacccc aaagactcca ccagcttcga acgcgaaaaa gaagactaca cccgcgattt
     8461 ggacaaaccg ctcaaaggcg tgaaaatcgg cctgcccaaa gaatacttca gcgaaggcaa
     8521 cagcaccgat gttcagacgg cattgcaaaa caccattgat ttgctgaaag cacaaggcgc
     8581 ggaaccggtc gaagtttccc tgccgcaaac caagctgtcc atccccgcct actacgtcct
     8641 cgcctccgca gaagccggca ccaacctttc acgttacgac ggcgtacgtt acggacaccg
     8701 tgccgcccaa ttcggcgatt tggaagaaat gtacggcaaa acccgcgccg aaggttttgg
     8761 cagcgaagtc aaacgccgca tcatgatcgg cacttacgta ctgtcgcacg gctactacga
     8821 tgcctattat ctcaaagccc aaaaactgcg ccgcctcgtt gccgatgact ttcagacggc
     8881 atttgcacgg tgcgacctca tcctcgcgcc gaccgcaccc tccgccgcac ccaaaatcgg
     8941 agcggatact tctccggttg aaacctactt gagcgacatt tacaccatcg ccgtcaacct
     9001 cgccggactg cccgcattga ccctgcccgc aggcttcagc ggcggcggac tgcccgtcgg
     9061 tgtacagctt gtcggcaact acttcgccga agccaaaatc ctcggtgcgg cgcatcaaat
     9121 ccaactcaac agcgattggc acggcaaacg acccgaatga agcagaaccg cacctttacc
     9181 ttccccgatt ttcgcaccgt ttacagctat gcgcctttat atcggctgca acatttaaaa
     9241 tacacattgc gaaaattttt cggaaaaaaa gaaatttacg ccttcgagca gtttgtcaac
     9301 gcctccccta tccgtcaggg gctgttcctc cactgcccgc aggatgccta tccgctgctg
     9361 cgcgaatttg ttgacaggcg ttttaactgc aaacgccgtt tagatgcgat gacggcagat
     9421 tttctcatgg cggaaaaact cttcggcaca gacgttctgt gccaaatgga agactgccgc
     9481 ttccatctgg tcttggcgca cctttcagac ggcatcagct tgtggctcaa ccgcaacgac
     9541 aactgcgtcg aagaaggcgc gtggtcttta tctttgcgcg atgaagcagg caaccggctg
     9601 tatatggcga ccttcgcctt tgtcggcaca cacctgctga cagcctccgt acaagggccg
     9661 tcaggtgaag aagccaaaga caccgtccgc cgcataacca aacaacttca cggcttacgt
     9721 ccccaacaac tgatggtaac cgcactgcaa tatttcgccg ccgcattaaa attagacggc
     9781 gcgataggca ttgcacaaaa acatcaagtc aaattacgct ggaaacttaa aaagcgcgtc
     9841 aaaatgaatt acgacgcatt ctggcaggaa tacggcgcaa gtttggaacg ggacggctac
     9901 tggcatcttc cccaaacccc cgcccgcaaa gaccttgccg acatcgaaag caaaaagcgt
     9961 tcgatgtacc gcaagcgtta tgaaatgctg gacgatatgg ttgcaaagat gaaggacagt
    10021 ctgaaaacag aagcacgcgg catttcagac ggcatccaaa cggaaaaacc gctccgccgg
    10081 acagtctgat gcgaagacta tcgaattgat attttagaga aagaacctcc tatgacttgg
    10141 gaaactgtaa tcggcttgga aatccacgtc caattgaaca ccaaatccaa aatcttcagc
    10201 ggtgcatcga ccgcattcgg cgcagaaccc aacgcgcacg ccagcgtagt ggaatgcgcg
    10261 ctgccgggcg tactgccggt aatgaaccgc gaagtcgttg aaaaagccat caaattaggt
    10321 ttggctttgg atgcaaaaat caaccggaaa aacgtgttcg accgcaaaaa ctacttctat
    10381 cccgacttgc cgaaaggcta tcaaatcagc cagttggatt tgccgattgt cgaacacggc
    10441 aaattggaaa tcgtggtcgg cggcgatgtg aaaaccatca acgtaacccg cgcccatatg
    10501 gaagaagacg caggcaaatc cgtgcacgag ggcttgaacg gcacgaccgg catcgacctg
    10561 aaccgcgccg gcacgccgct gttggaagtg gtatccgaac ccgaaatgcg ctccgccgcc
    10621 gaagccgttg cctacgccaa agccttacac agcttggtaa cctggctgga catttgcgac
    10681 ggcaatatgg cggaaggttc gttccgcatc gatgccaacg tatccgttcg cccgaaaggc
    10741 caagcggaat tcggcacgcg ccgcgaaatt aaaaacctca attccttccg tttcttggat
    10801 caggcgatta attacgaagc cgaagcgcaa atcgagattt tggaagacgg cggcacagtt
    10861 caacaggcga cgatgctgtt cgacccggaa aaaggcgaaa cccgcgtgat gcgcctgaaa
    10921 gaagacgcgc acgactacgg ctacttcccc gatcccgatt tgctgcccgt catcatttca
    10981 gatgcccaaa tgcaaaaagc caaagcagaa atgcccgagc tgccgaaaga aatggcggcg
    11041 cgtttcgtgg cggattacgg cgtgtccgaa tacgacgcgc gcctgctcac cgccagccgc
    11101 gtgcaggctg cctattttga agaagccgcc aaagagagcg gacaaggcaa gccgactgcc
    11161 aactggatga acggcgaact tgccgctacg ctgaacaaag aaggcatgga acttgccgac
    11221 agcccgatta ccgccccacg ccttgccgcg ctggtgggca aaattgccga tggcacatta
    11281 agcggcaaac tggcgaaaaa agcctttgaa gccatgtggg cggaaccgga aaccagcatt
    11341 gccgaaatca tcgaaaaaca cagtttgcaa cagatgaccg ataccggcgc ggttgaagcg
    11401 atggtggacg aagcgctggc aaacaacgcc aaagccgtgg aacagtttaa atccggcaac
    11461 gaaaaagccc tgaatgcgat tgtggggcaa gtgatgaaga ccagcaaagg caaagccaat
    11521 cccgcgcagg tgcaggagtt gattaaagcc aaacttgcct gataagcaag atgccgtctg
    11581 aaacccaaat ccgacgttca gacggcattt tcacagtctg accacattaa aacagcataa
    11641 aaaacaatat tccatgaacc acaccgttac cctgcccgac caaaccacct ttgccgccgg
    11701 cgacggcgaa accgttttgt ccgctgccgc ccgtcaaaac ctcaatctgc cccattcctg
    11761 caaaaacggt gtctgcggac aatgcaaagc cgaactggcc agcggcgata ttcaaatggg
    11821 cggacactcg gaacaggctt tatccgaagc agaaaaagcg caaggcaaga ttttgatgtg
    11881 ccgcaccacc gcgcaaagcg atatcaacat caacatcccc ggctgcaaag ccgatgccct
    11941 acccgtccgc accctgcccg cacgcatcga aagtatggtt ttcaaacacg atgtcgccct
    12001 cctgaaactt gccctgccca aagccccgcc gtttgccttc tacgccgggc aatacattga
    12061 tttactgctg ccgggcaacg tcagccgcag ctactccatc gccaattcgc ccgaccaaga
    12121 aggcattttg gaactacaca tccgcaggcg cgaaaacggt gtctgctcgg aaatgatttt
    12181 cggcagcgaa cccaaagtca aagaaaaagg catcgtccgc gttaaaggcc cgctcggttc
    12241 gtttaccttg caggaagaca gcggcaaacc cgtcatcctg ccggcaaccg acacaggcta
    12301 cgccccatcc gcagcatcct gctcgacctt atccgccaaa acagcagccg cgctgcccat
    12361 ttctactggg gcgcgcgtca tcaagatgat ttgtatgccc tcgaagaagc acaagggttg
    12421 gcatgccgtc tgaaaaacac ctgcttcacc cccgtattgt cccgtcccgg agagggctgg
    12481 cagggaagaa aaggccacgt acaagacatc gcggcacaag accaccccga cctgtcggaa
    12541 tacgaagtgt ttgcctgcgg ctctccggct atgaccgaac aagcaaaaaa tctgtttgtg
    12601 caacagcata agctgccgga aaacttgttt ttctccgacg cattcacgcc gtccgcatca
    12661 taattcctcg gtataaagag gattcgggct ttccgttcgg aacacaaaaa acttcccgtc
    12721 cgtattttcc ccgtgaaaaa atgccgtctg aaacctgatt ccggttttca gacggcatat
    12781 gttttttcct gttcaaggcg acaaccgctc gcgtatccag ccgccatcca gcaaacggta
    12841 ttggatgcgg tcgtgcagcc tgctcggcct gccctgccaa aactcaatca aatcgggaat
    12901 cacaatatag ccgccccaat gcggcggacg cggcacgtgc aaaggatgtt tgagtccgac
    12961 cgccgccgcc tttgccacca ataccgcctt gttcggaatc acctcgctct gcgcgcttgc
    13021 ccacgcaccc aaacggctct gatacgggcg actctcaaaa tattcgtccg acaacttctc
    13081 cgccagcctt ccgacacgcc cttccacgcg cacctgacgc tccagctccg gccaaaaaaa
    13141 cgtcatcgcc gcaaacggat tgagttccag cgaacgcccc ttgcggctgt ggtaattcgt
    13201 aaagaaaaca aaaccttctg aattaacctc cttcaaaagc accatacggc tgttgggcct
    13261 cccgcgcccg tcgaccgccg ccacattgac cgccgtcggc tcgttgacct cggcgcgtac
    13321 cgcctcgtcc aaccaccgct cgaactgctc gatcggatta tcggcgcaat cggcttccga
    13381 caattcccgt ttgctgtaat cttcccgaat attgtgcaaa tccatttacc gctcctctta
    13441 tcatttgaaa gattctactc ccgcacgcaa accgatttca accgttgcac aaactttgtc
    13501 cccaaagccg cagcgacgat ttcatccgca aaaccgccgc atcaggtaca atatcgaacc
    13561 gtccgaccga ggacggcatt ttatcaaccc gtcctgccgc acacgccgca gaagaaccgc
    13621 cttatcaggc gagttaggaa aaatgatgtc caagcaaccc accagcaaac gccaatggcg
    13681 cgacggcgca gccccgtctg ccaagaaaac cgccaaacct ttcaaaagca aagcccgtcc
    13741 caaagacgaa acgcgcaaaa ccgccgccca agcttacgga caaaaagctt cagacggcat
    13801 caagctgcaa aacgccccca aacagcgcgc cgccaaagcc aaaaaactcg tcgtccgcaa
    13861 tcccaaccaa aaaattatgg aacacgcgcg cgatttgaaa gaacgccgca gcgacctgtc
    13921 gcgtatggaa cccgaacgcc tgcaaaaagt gcttgcagcc tccggcgtcg gctcgcgccg
    13981 cgaaatggaa gaatggatca acaacggctg ggtaacggtc aacggcaaaa ccgcgcaact
    14041 gggcgacaaa gttacccccg acgaccacgt taccgtcaaa ggcagcatca tcaaactcaa
    14101 atgggccgac cgcctgccgc gcatcatcct gtattacaaa caagaaggcg aaatcgtttc
    14161 ccgcgacgac ccgcaaggcc gcgtcagcat attcgaccgc ctgccgcagg ccgccagcag
    14221 ccgctgggtc gccatcggac gcttggacat caacaccagc ggacttctga ttctcactac
    14281 ctccggcgaa ctcgtccaac gtttcgccca ccccagcttc gaagtcgaac gcgaatacgc
    14341 cgtgcgcgta ctgggcgggc tgactaccga acaaatgcgc agtctcaccg aagaaggcgt
    14401 gatgctcgaa gacggcttgg caaaagtcga acgcatccgc gaacaaggcg gcgaaggcgc
    14461 aaacaaatgg tacaacatcg tgattaaaga aggccgcaac cgcgaagtgc gccgcatatt
    14521 cgaaagccaa ggcctcacag tcagccgcct cgtgcgcgtc gccttcggcc ccatcggact
    14581 gcccaaccgc ctcaaacgcg gacaattcta cgaactcaac cccgccgaag tcgccaacat
    14641 cctcaaatgg gcggatatgc tgctgccggg cgaacgccgc cgcaaaaaag cctgaaagta
    14701 aaaatgccgt ctgaaacacc tgctgtttca gacggcattt tattcgggcg ttttcaggag
    14761 aaaaggtcga gtgctttgac aaagaccatc accacgccgt aggcgagcgg tatgacggcc
    14821 aatgcccaac gccaccagac cgatatgccg tcgccggaaa ctttttcgcc cacaaggtaa
    14881 gcgtcggata tggcggtttc gtcgtcgggg ttgccgctgc gtgcggcggt tttgatgtct
    14941 ttttcgtggt gtttttcgtg tacggatttg acggcgaggt tgcacaacaa accgataatc
    15001 agcaggcccg ccataatgta catggttacg ctgtatgcct gtgccgccgg tatgccgctg
    15061 tcgatttggc tttggcgtat gtaattgacc agtaccgggc cgatgacggc ggcggttgac
    15121 caggccagca ggatgcgccc gtgaatcgcg ccgacctgat aggtgccgaa caggtctttc
    15181 aggtaggcgg gaatggcggc aaatccgccg ccgtacatgg aaataatcac gcaaaagccg
    15241 atgatgaaca gggctttgct gccgccttcg ccgatggagg gaacggcgaa atacagcagc
    15301 gaaccgagta cgaagaagat ggtgtaggtg tttttgcgtc cgattttgtc ggaaacgctc
    15361 gaccacaaaa agcgtccgcc catattaaac aggctcagga ggctgacgaa gcctgccgcc
    15421 gcacctgcgc cgactgccga ctgcctgcct gcggaggttt cggaaaagag ttcctgaatc
    15481 atcacggatg cctggcccaa tacgccgatg ccggcggtta cgttcaggca caatacccag
    15541 aacaacagcc aaaactgcgg cgttttcatg gcttgggaca cgttgacatg attgctgctg
    15601 accggcttgt tttgcgtttt cggcacggta tagccttcag gtttccagcc gtcggcaggt
    15661 acgcggatgg taaacgcgcc gaacatcatc agtgcgaggt aaaacaggcc caatacggca
    15721 aaggtttcgg caaccccgac cgaagcggcg tttgaaaagg cgttcatcag tgatacggaa
    15781 agcggcgagg ccagcattgc gccgccgccg aaacccataa tcgccaaacc ggtcgccata
    15841 cccggcttgt cgggaaacca tttcatcagc gtggaaaccg gcccgacgta gcccaaaccc
    15901 aagcctacgc cgccgatgac gccgttgccc aaataaagca ggaagaggtt gtgcgtacgc
    15961 acgccgaatg cagatacgaa gaagcccagg ctgaaacagc aggcggcggc aaatatggct
    16021 ttgcgcggcc ctacccgttc catccatgtg ccgaacaggg cggccgacgc gcccagcatc
    16081 gcgagtgcga tactgaaaat ccaacctacg gtcgtcagct tccaatctcc ggccgccgat
    16141 tcggttatgc cgataagttt ggtcagcggc gcgttgaata cggaataggc gtaaatctgc
    16201 ccgatggcaa ggtgcaccgc taatgctgcg ggcggcacga gccaacggtt gaaacccggc
    16261 ttggcaatgc ttgcctcacg gtctaaaaac ttcataacat cctctttctg tcagttgaaa
    16321 aataaaattt catttgccca atggaaactt attaaaaatt ataaaaaaat atcgggttgg
    16381 gtttttatcc gccccaagat gcgccgtctg aaacatttcg ggtgtgcgga gaggtttctg
    16441 ttttttccga caaattcctg cggctttccg cttccggatt cccgcttttt agggactgga
    16501 ttcccgtttt cgcgggaatg acggcggcag gttgctgttt ttttgtttat aaatttctgt
    16561 aactttgaat ttcagccatc ttcaataaat tatcgaaacg tatcaatcgt cattcccgcg
    16621 caggcgggaa tccgtttttt gagtttcagt catttctgat aaaccccggc ggctttccgc
    16681 ttccggattc ccgcttttgc aggaatgacg aattaaagat tatcttaagg tcaagggact
    16741 gggttcccgc tttcgcggga atgacggcgg agggggagcg gtttttccga ttgggtttaa
    16801 atgcaatcga acaaatcctg ctgcccttgt tctttgctta cgcgcacgtc ggtttcgccg
    16861 tcggagaagg taatgtgcag cttctgcccc tgcttcaaaa catcggcgtt acggatgact
    16921 tgtccgcgtg tatttttgac aacggagaag ccgcgttcca gaatgtgctg cggcgaaacg
    16981 gcttcgagca atgcggcttg ggcggtcagg ctttgacggc gacgggcgag cagccggctg
    17041 aaggcggcgg gcaatgccgt ctgaaggcgg acgatatctt gccggtaaac ggaaacatca
    17101 ggacggcaat gttgcagggc ttgggtttgg cgttcaaaac gggcggtgtg ggcgcggagg
    17161 ttttgcgtca tcgagtaaga cagcgtttgc gccagcttgc cgattgaagc gcgctgctcg
    17221 tcaagttttt ggcgcgggtg gcggatttgc cgcgcgagcc agtcgagttt ttggctggca
    17281 tcgaaatagc gttgctccaa aacggttttc agacggcctt gggcttggac gaggcggtgc
    17341 agtgattctt ggcggttggg gctgaccagt tccgccgcgc cggtcggcgt gggcgcgcgc
    17401 acgtcggcaa cgaaatcggc gagcgtgaaa tcggtttcgt gtcccacgcc gctgacgacg
    17461 ggaatcgtgc aggcttcgat ggcgcgcacg accggttctt cgttaaacgc ccgcaagtct
    17521 tcgatgctgc cgccgccgcg acagacaatc agcacatcgc attcggcgcg ttgcgatgcg
    17581 gttttaatcg cttgggcaat ctgaaacccg ctgcccgcgc cctgaacggc ggccggataa
    17641 acgatgacgg ggatttcggg cgcgcggcgt ttcaaggtgg ttacgacatc gcgcaaagcc
    17701 gccgccgcca gactggtaac gatgccgata cattgcggac ggacgggcaa aggttttttg
    17761 cgttccgccg caaacgcgcc ttccgcctgc aactgcgcct tcaaccgctc gtaggcttcg
    17821 taaagctgcc ccaaaccttt gagccgtact tcgtttacgg taatctgaaa ttcgccccgc
    17881 gcctcgtaaa tactgatttt gcccgccact tcgatatggt cgccttcttt caaaggttgc
    17941 gccaaacgcg ccgccgcacc cttgaacatc gcgcaacgca cctgcgcgcg gctgtctttg
    18001 agtgagaaat aataatgccc gctggcggcg cgggtcagat tggacacttc gcccgcaatc
    18061 cacaggccgg caaggtggtt ttccaaaata cttttggcaa atgcgttcaa ctcggaaacg
    18121 gacaacacgt cagaatgaaa aaaatcagac atcgaatcaa tcaaataata aaaaatatga
    18181 atatgttttg aagcccaagg cggcatcgtg ccgtctaaat tgtcaacaat attataacac
    18241 gcgccatctt gccgccagcc ttttcctata tgactttttt aagcggggaa tgggaaaaat
    18301 attcatcaac ccgccctcaa tctattcaaa ttgcaccgcc ggcaggctat gatgcggata
    18361 ttttcgacag gaggaaaaat ggatacgcag gcagttatca cacatattgt ccgatggttg
    18421 gacgaatacg ccgctcaggc aaatgcaaaa gggttcgtcg taggcgtttc cggcggcatc
    18481 gattccgccg tcgtctccac gctcgccgcc cgcaccggcc gcaccacgct gcttctggat
    18541 atgccgatac gccaacaccc cggccagctc gagcgggcaa ggcggcacat ccgcaatctg
    18601 caagggcaat atgccaatgt aagcgcgcaa acggccaatc tgaccgacac cttccaaacc
    18661 tttgagcaaa ccgtcggcgt tcatcagacg gcatttgcca atcagccgct ttcgctcgcc
    18721 aacgccagaa gccgcctgcg tatgctgacc ctgtactact acgggcagat acacggactg
    18781 ctggttacgg ggacaggtaa taaggttgaa gatttcggcg tgggtttttt taccaaatac
    18841 ggcgacggcg gcgtggacat cagcccgatt gccgacctga ccaaaacgca ggtttaccgg
    18901 cttgccgaag cattgggcgt ggacgaggcg attcaaaaag ccccgccgac cgacggcctg
    18961 tgggatacgg aacgcaccga cgaggaacag atgggcgcaa gctatcccga actggagtgg
    19021 gcaatgggcg tgtacggcac gcgcaaaccc gaagattttg aaggacggcg gcgcgaagtt
    19081 ctggaaatct atacgcggct tcaccgcgcg atgcagcaca aaatcaaccc gattcctgtg
    19141 tgccgcattc cgcccgaatt gctgggctga aacatgaaaa tgccgtctga aacggaaaaa
    19201 ccgtatttca gacggcattg aaatatccga ctcctatccc ttaagaatcg agtacgcggg
    19261 caaacagaat atcgttttcc aaatgaatgt ggtcgttcaa atcgtccgcc atttcttttg
    19321 ccagcgcgta aaggcgcgtc caacttccgc aagccccttc gggtgcgtgg aaattaccgg
    19381 tcagctcttt gagccgtgcg atggcgcggt cgtgttcttc atgttcgtgc atcatcacgc
    19441 tgatgggcat cgccgcaccg cgtccgacac cctgattaat catcggaaac agcatccgct
    19501 cttccttcat catatgcatc agcagctcgt cccgcatatc ggcaagcagc ccggcaattt
    19561 ccgccggaaa ggtatcggca tggacttggg ccactttctg cgccagcggc accaattctt
    19621 caaattgtgc gcggtggaca ttgtggtagc gttgcaggat atgatcaacg gttgcgccaa
    19681 agggagcagc ttcccaaacg gaaaaatcag tcattgcagt gttcctttta tagggtttcg
    19741 ggtttggttt tgaacattca tactttaaga atctatttaa acggagcata caccgcccgc
    19801 gcgtttctgt acagcctcaa aaacattcct tatactttga taataaaagt aattttcaaa
    19861 aataaaatac tgtccgaacc gttttttaga atttgcaaag gcgattgggg cggtacagaa
    19921 aaactattat cccgcccgcc cacttgaaat ttttatgccc aagccctatc ctgcacgcta
    19981 tcgtgcgaat cccaaccgaa aaggaaaaat aatgagcagc gaattaatcg tacacaccgg
    20041 cgatgccgcc ttcgagcaag atgttttgaa atccgacctc cccgtcctgc tggacttttg
    20101 ggctccgtgg tgcggcccct gcaaaatgat tgccccgatt ttggacgaca ttgccgccga
    20161 atttgaaggc cgtctgaaag tagtcaaaat caacatcgac gacaacgaag ccaccccgtc
    20221 ccgtttcggc gtgcgcggca ttccgaccct gatggtgttc aaaaacggcg aagtcgtcgc
    20281 caccaaagtc ggcgcattgg caaaaggtca gctgaccgcc tttgtcgaag cctccatcgc
    20341 ctgataaagc gcaatcgaaa agccgccgga agattccggc ggctttttcg cacccttaag
    20401 atttgtggcg gatttcccaa caccggtgga tttttttgtt gcggaaatct tcgggaacgg
    20461 attgtttgga aatgtctttc acggcgtatt gttccgatac cgaatcgtcc aaaacgaagc
    20521 tgcgcaggtt gttggaaaaa tacaaaatgc cgtctgaagc gagcaggttc accgcgccgt
    20581 ctatgagttt ttgatggtcg cgctggatgt cgaggatgcc gggcattttc ttgctgttgg
    20641 aaaaactggg cgggtccatc acaatgaggt cgaactgcct gccttcccca tatgccgtct
    20701 gaaggtattg gaacacgtcg gcgcggacga ttttgtgccg ttccatatcg atgccgttca
    20761 attcaaaatt gcgcctcgcc caatcgagat aagtgttgga caaatcgacg gtttcgctgg
    20821 atgccgcgcc gccggtggcg gcatagacgg tgaagctgcc ggtgtaggaa aacaggttca
    20881 aaaaacgttt gcccgccgcc gtttcgccga cttttttgcg cgtgttgcgg tgatcgagga
    20941 agaagccggt gtcgaggtat ttgtcgaggt tgacccaaaa cttgcggccg ttttcggcaa
    21001 tgacgaaatc gtcgcccgcc ttgccggttt tctcgtactg ctgcaaacct ttttggcgtt
    21061 cgcggcgttt gagacggatt tgttcgggtt caaaaccggt aacgaaagcg acggcttcca
    21121 atacttcggc aagccacgct tcgtattctt cgggccgcat cagccagccg gtatcgtatt
    21181 cctgaaggtg gattcgatcg ccgtaaacat cggcggcaaa agggaattgg gggatgtcgc
    21241 ggtcgtaaat gcgccaagct tcgatgccgt tgcgtttcgc ccatttcata aggtgtttga
    21301 tgtttttgcc caagcggttg gcaaacggtg tgatgtcggt cattgatttc aggcggaata
    21361 aagtggaaaa cggaaatttt actgtaatta acgcccgatt gcttgacctt ttcgggcaaa
    21421 ccctatacca ttctccgttt atatcgtcat acgaagccat cgccttccaa cctaaaccac
    21481 ccttacgggc gcgtttcttt tgttgctttg atcttgcaaa gcgtatctgt gcaggttgcc
    21541 gtcgatgtaa accacaagca agccgcttgc gacaaccctg taacttcaca ttccccgtat
    21601 cgttaccctt ccctgcttca ggccgtctga acctttcgga cgcgggcgtt gttgtcttcc
    21661 aaggatagcc atgtctatta aatttgccga tttgaacctt gataaaaaca ttttgtccgc
    21721 cgtcagcagc gagggttacg aaagcccgac gccgattcag gcgcaagcta ttccgtttgc
    21781 tttggaaggc tgcgacatca tggcttcggc gcaaaccggc tccggaaaaa ccgcagcctt
    21841 tctgttaccg actttgcagc gactgaccaa acgcagcgaa aaaccgggca aaggcccgcg
    21901 cgcgctggtg ttggcaccga cccgcgaact ggcggcgcaa gtcgagaaaa acgcgctggc
    21961 gtatgccaaa aatatgcgct ggttccgcac cgtcagcatc gtcggcggca catcgttcgg
    22021 ctaccaaacc cgcgccctga gcaaaccggt cgatttgatt gtcgccactc cgggccgtct
    22081 gacggacctg atgcaaagcg gcaaagttga ttttgaacgt ttggaagtgc tgattttgga
    22141 cgaagccgac cgtatgttgg atatgggctt tatcgacgac atcgaaacca tcgtggaagc
    22201 caccccggcc gaccgtcaga ctttattgtt ctccgccact tgggacggcg cagtcggcaa
    22261 actggcgcgc aaactgacca aagactctga aattatcgaa gtcgaacgcg tggacggcca
    22321 aggcaaaatc gaagagcagt tgttgtactg cgacgatatg cgccacaaaa accgcctgct
    22381 cgaccacatc ctgcgcgatg ccaacatcga ccaatgtgtg attttcacgt ccaccaaagc
    22441 gatgaccgaa gtcatcgcgg atgaattgta cgaaaaaggt ttcgccgcca actgcctgca
    22501 cggcgatatg ccgcagggct ggcgcaaccg cacgctgacg gatttgcgta aaggccgctg
    22561 caaaattttg gttgccaccg acgttgccgc acgcggtatt gacgtaccga ccattaccca
    22621 cgtcataaac tacgacctgc cgaaacaggc ggaagactac gtccaccgca tcgggcgcac
    22681 cggccgcgca ggccgcacgg gtattgcgat tacgtttgcc gaagtgaacg aatacgtcaa
    22741 agtccacaaa atcgaaaaat acattaaccg caaactgccc gaactgacca tcgaaggcat
    22801 ggagccgacc cgcaaacgca aatccgcaag cggcaagccg aaaggcaaag gcggctgggg
    22861 cgataggaaa tccggcggcc ggcgcggcga tcataaaccg ggcaaagaag gcttcggcgg
    22921 caaaacgcgc ggcgaaggtt tcaagaaaga aggctttaag aaagacagtt tcaaaaaaac
    22981 cggcgaaggc ttcaaaggca aacgcaaagc cggcgattct tttgcaggca aaagcgaacg
    23041 ccgttacaaa gaccgctaag ccccaacctg ccgcgtaaac caatgccgtc tgaaaccgat
    23101 ttcgagtttc agacggcatt tttgtaatgt ttcagcaccg cccggctttg atacccaaag
    23161 gattaatctg caatatagtg gattaaattt aaaccggtac cgcgttgcct cgccttgccg
    23221 tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactataa
    23281 aaaccctttt ccgctttggc aacgattgaa aatttccgta aattcaaata tctagattcc
    23341 ttcctgcgtg ggaatgacgc agaagggttt cagatgcagg atgggcattc ctgcctaccc
    23401 aatctcgccc tacggcttga cggttcgata tgatgccgtc tgaaaaccca acggcggtat
    23461 gacaatgccg ccctgccaac gcacgtagat cagaattgcc atcccgacat caaacgcttg
    23521 gaaacaaaat gccgtctgaa aatcaaacgg caacataaca atgcccctaa caaatacaaa
    23581 aatgccgtct gaaagccctt cagacggcat tggcgcgccg ggtttaccgc cttctgccga
    23641 aaccgcgcat agcgggacgg cggtaattga cgggcgggcg cgttgtcggg cggtaacgct
    23701 gcgcctgcgc cgcctgttgt tttgcacgga ggctgcgcgt gttcaaatcc ctgctggtgc
    23761 gcgcattagg gcgtgcggac tgatggtagg cagcacgcgc gcgacgggcg acggggctgt
    23821 cttggttgcc cgcccgtgtg aatttgtttg ccagcgcgtt gccgataaac gcgcctgccg
    23881 ccgcgccaat caggctttgc agcagccagc ttcctgtcga ttggtcgtaa atatactgct
    23941 gcccgtcttt gccggtaacg ggctgaccgt tgttgccgtt tgcctgcgcc tgggcaggaa
    24001 tggtgtcttt gactgcttcg ggagtcagtt ggtaaaccgt atcgtctgcc tgctgtgcga
    24061 gctgctgttg cagggcttca atctgtttct gctgccgttc gagccgcgcc tgcgtgtcgt
    24121 cttggcaggc ggtgagtgcg aatgttgcga taagcgcgga ggcgatgatt tttttcatgt
    24181 gtgtcctgtt cgggtggaaa atcggttgta ttgtatcgcc gtcgggaatt ttggcaagca
    24241 ttctgccggc aaatcgtgat gtttacaggg gtagggtgtg cagtttgcgg acaaatgcga
    24301 ggctgttggc gacagggttg cctttgtttt cgacttcgtg ttcggtttct atggtcagca
    24361 ggcggcggtt tttgtgtttg ttcaggctca attcggcttg tgcgggtgtg aaaaacaggc
    24421 ggtgtttttc ggcaaagcgg caggcgcgct tgtttgcggt tttcaaaatc tgaagcagcg
    24481 atacgtccag atggaagcgt cgcacgccca atacgagaat ccgtgcggca aaaaaaatcg
    24541 gcggcatcgg taaactcggc ggggctgctg cggctgaagt cgtgccgttc ggcggctatc
    24601 agggcggcaa cggtgcggat ttgcggaatc atcgattcgc tgatgagcgt gtcgtcccgc
    24661 cctgcatcga gaagcatggg ggaaaaatcc tgcgcatcat cgacaataat gctgcaagtg
    24721 tgcaggtttt cgttttgtac ggcggtttgg ggcattgcat ccatggtcat tttcctgatt
    24781 ctgtcgtgtg ttgccgaatc gggcgacctg tgtgaaggta acaaaaaagc cgccccgttt
    24841 tcgagcggcc tgttttgcgt atgggatgga tttcaagcaa gcgcaaaaaa gcaccgcacg
    24901 tctgtgtggt accaatagca gtaagcggtt gtaaacgttt tgccttgcat gatgaaatgc
    24961 cgtccgaaga taaaaatatt ggggagattc taaatcaaaa cgctgccgcg cctcaagcat
    25021 tatatcgaat tttttttgtt ttttcagcta tccggttgaa aatatttagg tttattttta
    25081 ccactgcccg atattgtcgg caatttccct ttatccgcct tgaaaaacgg tgcataatcc
    25141 cgaacaaaac cgcaatcagg agcaattatg caaaactatc tgacccccaa tttcgccttt
    25201 gccccgatga ttcccgaacg cgcttcaggc agccgcgttt gggatacgga agggcgtgaa
    25261 tatattgatt tttcaggcgg catcgccgtt aacgctctgg ggcactgcca ccctgccctt
    25321 gtcgatgctt taaacgcgca gatgcacaag ctgtggcaca tttccaatat ctatacgacg
    25381 cggccggcgc aggaattggc gcaaaaattg gtgaaacaca gttttgccga caaggttttt
    25441 ttctgcaact cgggcgcgga agcgaatgag gcggcgttaa agctggcaag gaaatatgcg
    25501 cgcgaccgtt tcggcggagg caaaagcgaa atcgtcgcct gtatcaacag tttccacgga
    25561 cgcacgctgt ttaccgtgtc cgtcggcgga cagccgaaat acagtaagga ttatgcaccg
    25621 ctgccgcaag gcattacgca cgttccgttc aacgatattg ccgcgctgga agctgccgtc
    25681 ggcgaacaga cctgcgcggt catcatcgag ccgatacagg gcgaaagcgg catcctgccc
    25741 gccactgcgg aatatttgca ggcggcgcgc cgtctgtgcg accggcacaa tgcgttgttg
    25801 attttggacg aagttcaaac cgggatgggg catacgggca ggctgtttgc ctatgaacat
    25861 tacggcgttg ttcccgatat tttgagttcg gcaaaagcct tgggctgcgg ctttccgatc
    25921 ggcgcgatgc tggcgacaga aaagattgcc gccgccttcc aaccgggcac gcacggctcg
    25981 actttcggcg gcaacccgat ggcgtgtgcg gtcggcagcc gcgccttcga catcatcaac
    26041 gcgccggaaa ccttgcacaa cgtccgcagt caggggcaga aacttcagac ggcattgctg
    26101 gatttgggca ggaaaacagg cttgttctca caggttcgcg ggatggggct gctgctcggc
    26161 tgcgcgttgg acacgcctta tcgcggacgc tcatccgaaa tcgccgctac ctccttgaaa
    26221 cacggcgtga tgatcttagt tgcgggtgcg gacgtattgc gtttcgcgcc ttcgctgctg
    26281 ttgaacgatg aggatattgc ggaaggtttg cggcgtttgg aacacgtgct gacggaattt
    26341 gccgccgcaa accgccccta ggcagaaagc agatgccgtc tgaagacggg aaggcctcag
    26401 acggcatcag aaacaaaaaa ccgcttcgga aacgtgatcc aacgttccga agcggttttg
    26461 tttgccatca ggactcgaac accaattccg gttccctgcc ctcttcgatg actgccttgc
    26521 cgaccaccac ttttttcaag ccttgcaaat cgggcaggcg gtacatcgta tcgagcaggc
    26581 agcgttcgac gatggaacgc aggccgcgcg cgccggtttt gcgttccatt gcctgccgcg
    26641 cgatggaacg caatgcgcct tcttcaaatt ccaacccgac gttttctatg ccgaacaagg
    26701 cttgatactg cttgaccaaa gcgtttttcg gctcggttaa aatattaatc agcgcgtcct
    26761 catccagttc ttctaaagtt gcaatcacgg gcaaacgtcc gattaattcc ggaatcaggc
    26821 cgaatttgat taaatcttcc ggttcgacga tgccgaacag cttggtaatg ccggcatttt
    26881 cgtccttgct gtgaacggac gcgccgaaac cgataccacc tttctcggtg cgctggcgaa
    26941 tcactttttc caagcctgca aacgcgccgc cgcagataaa caggatgttg gcggtatcaa
    27001 cgttgataaa ttcctgattc ggatgcttgc gtccgccttg gggcggaacg cttgccaccg
    27061 taccttcaat cagtttcagc aaggcttgct gcacgccttc gccggacacg tcgcgcgtga
    27121 tggacgggtt gtcgctttta cgcgaaattt tgtcaatttc gtcaatatag acaatgccgt
    27181 gccgggcttt ttcgacatcg aaatcgcatt tgcccaacag tttggtaatg atttgttcga
    27241 catcttcgcc gacatagccg gcttcggtca gcgtggtcgc atccgccatc acgaacggca
    27301 cgtccagttt gcgtgccaaa gattgcgcca acagcgtttt acccgatccg gtcgggccga
    27361 taagcaggat gttggatttc gacaattcga caccgccgtc ggctttcggg tggcgcaggc
    27421 gtttgtaatg gttgtaaacc gcaaccgcca gcgccttttt cgcctgcccc tgcccgatga
    27481 cataatcgtc gaggttggca acgatttcgg cgggcgtggg cagtttgccg gattcttccg
    27541 gctcccctcc ggcattttcc ggaggcgtgc cgccgttgtc gccttcgtac aatatttcga
    27601 ggcagtttga gacgcattcg tcgcagataa aggcgttttc gccctcaatc aaatgtttga
    27661 cgtgtgattt ggattttccg caaaaggaac aggtacggtt ttcgttggac atggctttct
    27721 ttacaatgtg tgcgttgcag aaacggcatg cgccgttcgg gttgccaagt ataataacta
    27781 tatccgtgct tatcaacgta ttaccttaaa atcccgccga ttcggctata atacgcccct
    27841 ttgcaaccgc cccggcggca gaaatgccgt ctgaaaccaa atctgaggat attcatgaga
    27901 aaaccccaac gcggctacgc ccgccaagac cgtgtcaaag aacaaattat gcgcgagctt
    27961 gccgaactcg tccgtaccgg actgaaagac ccgcgcgccg gcttcatcac tatcaacgaa
    28021 gtcgaagtta cccgcgatta cagccacgcc accgtgttct acaccgtttt gaaccaagat
    28081 acgcgcgaaa ttacggaaga agtgctggaa cacgcgcgcg gacacctccg cagcgaattg
    28141 tccaaacgca tcaagctgtt caaaatcccc gaactgcatt tcaaatacga cgaatctttg
    28201 gaacgcggta tgagcctgtc cgcccttatc gatcaagtgg cggcggaaaa accggttgaa
    28261 gactgatact gccagaatga acaatctgat tcccgaacat ttagccgcct atgcacatag
    28321 tgataacctg caaattgaag gcgggcatcg ttgcttttca ttatcctgcc aaggtagaga
    28381 tactttccac atccgttact atggagagcc ttttgatgga ttgattaccg atactgataa
    28441 ggcgccggta aaaattgtgg cggtagaagc tgtaagcggc gatgaaatcg tattgtttga
    28501 tggggcggaa catggctata acgctatgtt ctgcgacaaa tatagccaaa atcaaaagca
    28561 aaacagaacg ttaactgatt tggatgaata tacttaccga gttccgattc atctttatta
    28621 caatatagac tacgaagatg aatatgaaga tttcgtcaat tctgaaggac aagtcccctt
    28681 aattgatggc cgcatcatta gttttgactc attaaaacga aatggctttg atgcaatcag
    28741 cattgatcta attgatgaaa aacactctgt tcgtgaatta ttgaatgaag aattaagctg
    28801 acacgcaggc cgtccgaacc ctttcagacg gtctgaacat taatattccc taattaatat
    28861 gaccaataaa cccgccaaac gcccggtcaa cggcgttctt cttctcgaca aacccgaagg
    28921 cctttccagc aacaccgccc tgcaaaaagc gcggcgtttg tttcatgccg aaaaagcagg
    28981 gcataccggc gtgctcgatc ctttggcaac cggacttttg cccgtctgct tcggcgaagc
    29041 ggccaagttc gcccaatacc tgctggatgc cgataaagcc tacaccgcca cgctgaaact
    29101 cggcgaagcc agcagcacgg gcgatgccga aggagaaatc atcgccgccg cccgcgccga
    29161 tatttcctta gccgaatttc agacggcctg ccaagcactg acaggcaaca tccgccaagt
    29221 gccgccgatg ttttccgccc tcaagcacga aggcaaaccg ttgtacgaat acgcgcgcaa
    29281 aggcattgtt atcgaacgca aaccgcgcga cattaccgtt tactctatcg atattgccga
    29341 gtttgacgcg cccaaagccg tgataagcgt gcgttgcagc aaaggcacct acatccgcac
    29401 cctcagcgaa ggcatcgcca aacacatcgg cacattcgcc cacctgaccg ccctacgccg
    29461 caccgaaacc gccggcttta ccatcgccca aagccacacg ctcgaagcct tggcaaactt
    29521 ggacgaaaca gaacgcgaca acctgctgct gccctgcgac gtattggttt cacactttcc
    29581 ccaaaccgtt ttaaacgatt atgccgtcca tatgctccaa tgcgggcaac ggccgcgttt
    29641 cgaggaagac ctgccttccg atacgccggt acgcgtttac acggaaaacg gccgctttgt
    29701 cggtttggcg gaatatcaaa aggaaatatg ccgtatgaaa gccttgcgcc tgatgaacac
    29761 ggcggcatcc gccgcctgag cggcggttaa aaatacgggc tgtgtttgaa taatatgttg
    29821 atatttccgc aaaatcccga tgcattcgga cacccgcccc gcttatcgca acgttgcgaa
    29881 cgcccccgga aacagcaaag acagcaaaga attgatttta ttataattta tttgtaaagc
    29941 catttgccgt tacacaagaa tggcacatta aaataactga tgaggattta taacgatgaa
    30001 gacaggcatt caaaccgaat tagcccaagc cctgctacca cacgaaaaaa tatgggcgaa
    30061 cgaagaaaaa accattttgg ccaaaaacat cctgttggat ttggtggaaa aaaccgaccc
    30121 gtccattatc ggtttgttat tgaataatga tgagttaaaa cgccatttct ttgtggaagt
    30181 gaatggtgtt ttggtgttta aattgcagga ttttcgtttt ttcttggaca aacacagcat
    30241 caataattcc tacacaaaat acgccaaccg cattggtttg acggacggca accgcttttt
    30301 gaaagacaat tcggatattg tgttggattt tccgtttaaa gattgcgtat tgaatggcgg
    30361 acaaagcacc gaagaaggcg aagaaattta ttttaaacgc aataatagcc agccagccag
    30421 ccagccagcc agccagccag ccagccagcc agccagccag ccagccagcc agccaattat
    30481 acaccaaatt aaccagaaaa agacaagaaa tcttttttaa tcaaacgctt gcttttgatg
    30541 aaattgaccg gctttttgac gcaaaagcat tctcaaaatt ctcccgccac accgcagacg
    30601 gcaaacaacc cgttggcgaa atcaaacgcc gttcagacgg tacgccagcc gaaaatctta
    30661 ttatcaaagg caataatctg attgccctgc attcgcttgc caaacagttt aaaggcaaag
    30721 tgaagctgat ttatattgac ccgccttatt attttgttaa aaagaaacca caagatagtt
    30781 ttggttataa cactaacttt aaattatcta cttggcttac atttatgaaa aatcgtttat
    30841 taattgccaa agaattatta actgatgacg ggattattgt tatttctatt gatgatgatg
    30901 gaaatgctta tctaaaaatt ttgttagatg aaatttttgg atttgaaaat tttatcggaa
    30961 atctgcctac tattatgaat ttaaaaggta ataatgatga atatgctttt gctggcactc
    31021 acgaatacac actagtcttt gctaaaaata aagataaatc tactttttat gaatttccaa
    31081 tagatgaaga taatttttta gagaaatggg aagaagatga aattgggttt tataaaaaag
    31141 gagctccaat gcgagcaaca ggaaccgaag ataaaagaga agatagacct gaaatgttct
    31201 atcctttttt ggtaaaaaat aacacagtta gtacaattac agatgaagaa ttttctcaaa
    31261 tctataataa agatttagaa gtattcaatg atgattttat tcaaaagcta aaagagaaat
    31321 acgaaaatct tggatacaat tttattttac ctattgctga taaacaatgg gggcgttggc
    31381 gttgggggta ttcaataaag aataaggcaa ggctgcagac ggatattatt gttagccaat
    31441 ctaaaaatgg aatttccttg tataaaaaac aaagacctga attagatgat ttacctacaa
    31501 aaaaacctaa aactattttt tataaacctg aatacagtag tggtaatgga actgaacaga
    31561 tgaaaaatct ttttggtgaa aaggcattta aaaatcctaa accagaagaa cttatccaag
    31621 attttattac catcacaacc aacgaaaacg acatcgttct agactaccac ttgggcagtg
    31681 gcacaaccgc cgccgtcgcc cataaaatga accgccaata catcggcatt gaacaaatgg
    31741 attatattga aacgcttgcc gttgaacgca tgaaaaaagt aattgacggc gagcaaggcg
    31801 gtatctcaaa agctgtgaat tggcaaggtg gcggcgagtt tgtttatgcc gaactttccc
    31861 catttaacga aaccgcaaaa caacaaattt tggcgtgcga aaattcagac ggcatcaaaa
    31921 cgctgtttga aggtttatgc gaacgccatt tcttgaaata caacgtcagc gtaaatgaat
    31981 ttagccaaat tattgaagag cctgaatttc aatccttggc attagacgaa caaaaacaaa
    32041 tgatgcttga aatgttggat ttaaatcaaa tgtatatttc attatccgaa atggacgatg
    32101 aacaatttgc aggctgcctg aatgatgatg ataaagcgtt aagccgtgcg ttctatcaat
    32161 cagtaaaaca tcaagcggag aaaaaagatg gcgaataata aaacgttgtt tgattgggtg
    32221 gaagaccgca aatcaatgct ggaagaaatg gaacagacgg attttttcgc gctgcctgaa
    32281 tttgtttcca ataatttaaa atatccgttt tttgaatggc aaaaatcggc tttagaaaac
    32341 tttgtgattt ttgaccgcac ttcaaaatta aaggatttcc ccgacatcaa aaaccgccct
    32401 acccatttgc tgttcaatat ggcaacaggt gcaggcaaaa cgatgatgat ggcggcattg
    32461 attctgtatt attttgagaa agggtatcgg cattttttat ttttcgtcaa taaaaacaat
    32521 atagtatata aaacggaaaa taattttatc gacccgacgc atccaaaatt tttatttacc
    32581 gagaaaattt tgcaaggtga tacggtaatt cccattcgca aagtggagac atttagccaa
    32641 tattcagacg gcattgaaat taaatttacc agcattcaaa aactatataa cgatattcac
    32701 accgagcgag aaaatcaaac cacattggcg gatttgcacg aattaaatct tgtgattctg
    32761 ggcgatgaag cacaccattt aaacgcgcaa accaaaggca aaaaacaagg cgaattagat
    32821 ttagaaaagg aaatgaacga gcgtaccagc gatgccgaaa ttgaacgcaa aggctgggag
    32881 catatggttt tggagttgtt actcaataaa aatggcaatc ccggccaaaa tgtgctgttg
    32941 gaatttaccg ccacactgcc tgaaaatgct gaagtgcaac aaaaatatgc tgataaaatc
    33001 atcacaaaat ttggtttaaa agaatttttg caaaaaggtt ataccaaaga aatcaatttg
    33061 gtatccggta cgctgaataa gaaagagcgg gtgttgcacg ctttattgtt tgcctggtac
    33121 cgccatcaaa tcgcgttgaa atacggcatt gccaatttca agcctgtgat gttgtttaga
    33181 agtaagacga ttgatgaatc aaaagcggat tattcggcgt ttttaaattg ggtagaaaat
    33241 gtgcaagcgg atgatttttc gtttttaact acattttcaa caagcttaaa cgatagcaat
    33301 aacgccaacg aacaaggcaa aacccgtacc gaacaagccc tgaaatttat gcaggaaaat
    33361 aaatttgaat ttgtgcattt ggcaaattgg attaaacaaa attatcaaaa acacaatgtc
    33421 attattacca actccgaaac caacaaaaac aaaactgaaa aaaccgacag cgaaaccgaa
    33481 aaattgctaa acaatttgga agcggctgat aatccgatcc gtgccatttt tacggtggat
    33541 agattaaccg aaggttggga cgttttaaat ttgtttgata ttgtgcgttt gtatgaaggg
    33601 caaaacggtg gcggttcgaa taaaaaatcg ggcaaaacgg ctgccgccac cgtatcggaa
    33661 aagcagttga ttggtcgcgg cgtgcgttat tttccatttg cgtttgaagg taaacagccg
    33721 aataaacgca aatttgacaa cgatatgcaa catgaattgc gtatcttgga agaattgttt
    33781 tattacacgc acgatgagca atcccgctat attacagaac tgaaaaacga gttacgaaaa
    33841 gacggttatt tgcctgaaaa agacgatgat aaggtattgg caacatttaa gctcaaatct
    33901 gaatttgcag ataataagga ttttagagag ttgttaattt gggcgaataa aaaaatcccc
    33961 aacccaaacg ccaaaagcaa taatgcagac agcctgcaag ccaatccgca aacgctttca
    34021 ttccaagttc acggtaatca actgttgcag gaaacgcaat ttacagccga tgaaaatgat
    34081 gaaacagccc gacaaatcgg cacacaaaat aattttactc aaaccataaa aatgagtgaa
    34141 atggagcggc atattttcaa taaagcatta catatcaaag ggaaaaatag tcaatcttta
    34201 ttccatttcg accgcctgca aagcaaactc gacattcaaa atcgcaatga attgcaaaat
    34261 aaattgttga aagactggca gattgaattt ttgggattag agcaagataa acaggttcgc
    34321 ccggatgata aacttgcagg ctgcctgaaa atcttggaaa tggttgaaaa acatctgaat
    34381 gaaagtgata taccgtttat cggtacaaaa gaatttacac ctaaaaaatt gtgggaaatt
    34441 ttcggcacgc caaaacaaaa atgggtcaaa aaagatgata taaaaactgc tattgccacg
    34501 caaaatgatt ggtatgtgat ggataatttt gccggaacgg gtttggaaga agcgttaatt
    34561 caatttattt cagcgcgctt gggcgatttg aaatctcaat atgacgttca tttaatccgc
    34621 aatgaagaag tgtttaaatt gaataacttt gccgatggcg aaggatttat gccggacttt
    34681 gttttattgc tgaaagataa acaaaaatct tcttccgaca gcgtggacga ctttttgcat
    34741 taccaaattt tcattgaacc gaaaggcggg catttagtgg aaaatgattc gtggaaagaa
    34801 gcttttttaa aatcaatcac agtggaatat gggagggata agatcctgca aaaaaataca
    34861 ccgcattatc gtttgatcgg tttgccgttt tttactgatc atcagaaaaa tggacaattt
    34921 acagagttat tccctttagg tgcggcatcg cttgaaaaat agagtagtgc attgcaggca
    34981 gccccgttcg gcaaaacttc ctttacaaaa gggcgttttg tcagatattt aatcaacaca
    35041 ttattaaaat acaaccaaat tttaatgccg tccgaaccat gtattcagac ggcatcgtat
    35101 ttttcagtat ctaaaccgtt tccctgcccc aatccttact cctcaaaatc gaggcatcga
    35161 catcttgaat atcgcggtgt cccgtaaacg ccatcgacac atccatttcc ttgtacaaaa
    35221 tctccagcgc gcgcgttacg ccttcttcgc catacgcacc caaaccatac aggaacgccc
    35281 gacctatcat cgtgcctttc gcacccaaag cccacgcctt caaaatatcc tggccgctgc
    35341 ggataccgct gtccatccaa acttcgatgt cgctgccgac tgcggaaaca atatcgggca
    35401 aggccttgat ggcggacaca gtatcgtcga gctggcggcc gccgtggttg gaaacgacca
    35461 atgcatccgc accgcttttc gccgcctttt ccgcgtcttc gggttccata atgcctttga
    35521 taatcagctt gccgccccac aaatctttaa tccgcgccac gtcgtcccag cttaagcgcg
    35581 ggtcgaactg ttccgccgtc cacgaagaaa gtgaagacaa atcgccgacg tttttggcgt
    35641 gtccgacgat attgcggaac gtgcggcgtt ccgtgttcag cattttcatg caccattcgg
    35701 gcttggtcgc cagattgatt aaattggcga tggtcggttt cggcggcgcg gacaggccgt
    35761 ttttgatgtc tttgtggcgt tgacccaaaa cctgcaaatc ggcagtcaat accaatgccg
    35821 aacatttggc atctttcgca cgcttaatca ggttttccat aaattcgcgg tcgcgcatca
    35881 cataaagctg aaaccaaaac ggcgcgctgg tgttctcggc aacgtcttca atcgagcaga
    35941 tggacatggt ggacaacgta aacggaatac cgaacttctc cgccgcacgc gccgccaaaa
    36001 tttcgccgtc tgcgtgtgcc atgccggtaa aacccgtcgg cgcaatcgcc accggcattt
    36061 tcacatcccc cccgatcatt ttggtttcga ggctgcgtcc ctccatattg accaatacct
    36121 tttgtcggaa gcggatatct ttgaaatccg aagtattctc cctataagtc gtttccgtcc
    36181 acgaacccga atcgatgtaa tcgtaaaaca tacgcggcat tttgcgcttg gcaacgcggc
    36241 gcaagtcttc gatgcaggtc attttgctca aatcacgttt catcttgttt ttccctgttt
    36301 aacagcctga tggaatatgc cgtctgaaac tgcttgagcc ttcaaacggc atttcgggca
    36361 atacccaaat aaatcactaa gatttagata atctatatca atattaaaaa taaatcaaat
    36421 cgttttgaaa ccgcttgccg gcaaaacgcc ccaaccgccg ctcctcaaaa aacataacta
    36481 actgattcaa aaaaccaaac aaataaagca aaatcagact ataccgccat ttaaacaaac
    36541 aatcggcaac agctccccca tacttgactg aaacactcag atattggaca attccgccca
    36601 ctataaaaaa gccgacacgg gcaaccacca ccatgagact gaccaccaaa gggcgtttcg
    36661 ccgttaccgc tatgctggat ttggcgatga acgcgcaaac cggcgccgtc aaactcagtg
    36721 ccatcagcga acgccaaaat atatccctct cctatctcga gcaattgttc ggcaaactcc
    36781 gccgcgccgg actggttgaa agcctgcgcg ggcccggcgg cggctacatc ctcgccgccc
    36841 ctcccgcaca aatcaacatc gcccaaatca tttccgccgc cgaagaccgg ctggacgcga
    36901 cccaatgcgg cagcaaagcc aactgccacc acggcgcgcc ctgcctgacg cacgaccttt
    36961 gggagaattt aaacaaaacc atcaacgact atctcggcag cgttaccctg caaagcatca
    37021 tcgaacagaa aaacaacggc gacggcagcc gcgtcgtcca atttacacac atccattaaa
    37081 taacacccga aaaagaaaga gcaaaccatg accgtcaaaa cccccgttta cctcgactac
    37141 gccgccacca cacccgttga caaacgcgtt gccgaaaaaa tgattcccta tctgactgaa
    37201 accttcggca accccgcctc caacagccac gcattcggct ggacggcaga agaagccgtc
    37261 gaaaaagccc gcgccgacat cgccgccctg attaacgccg accccaaaga aatcgtcttc
    37321 accagcggcg cgaccgagtc cgacaacctc gccatcaaag gtgcggcaaa cttctacaaa
    37381 accaaaggca aacacctcat caccgtcaaa accgaacaca aagccgtgct cgacacgatg
    37441 cgcgaactcg aacgccaagg ctttgaagtt acctacctcg gcgtgcagga aaacggtttg
    37501 attgatttgg aagaactcaa agccgccatc cgcgacgaca ccatcctgat ttccataatg
    37561 tgggtgaaca acgaaatcgg cgtggtgcaa aacattcccg ccatcggcga aatctgccgc
    37621 gaacgcaaaa tcgccttcca cgtcgatgcc gcccaagcct gcggcaaagt gcctgtcgat
    37681 gtcgaagccg ccaaaatcga cttgctctcg atgtccgcgc acaaagtgta cggccccaaa
    37741 ggcatcggcg cgctgtacgt ccgccgcaaa ccgcgcgtcc gcctcgaagc ccaaatgcac
    37801 ggtggcggtc acgagcgcgg tttccgttcc ggcacattgc cgacccatca aatcgtcggc
    37861 atgggcgagg ccttccgcat cgccaaagaa gaattggcac aagacacagc gcactacctg
    37921 aaactgcgcg acatcttcct caaaggcatc gaaggcatcg aagaagtcta tatcaacggc
    37981 gacctcgaac accgcgcccc gaacaacctg aacgtcagct tcaacttcgt cgaaggcgaa
    38041 agcctgatta tggcggtgaa agaactcgcc gtatccagcg gatccgcctg cacctccgcc
    38101 tcgctcgaac ccagctacgt cctgcgcgcg cttggccgca acgacgaact ggcgcactca
    38161 tccctgcgca tcaccttcgg ccgcatgacc accgaagaag aagtgcaatt cgcggcagaa
    38221 ctgatcaaat ccaaaatcgg caaactgcgc gaactgtcgc cgctgtggga aatgttcaaa
    38281 gacggtattg atctgaactc gatcgaatgg gcggcgcatt aagattgatg tcaaacaaag
    38341 gctgtctgag aaagaatacc atgaaagtta aaattattaa tttgccaaat ggttataaac
    38401 gtattattta tggaaaatat tttgaacaat ttgacttaga ttatgaacaa gatttagacg
    38461 tattaaaaaa agatattgaa tttgctttat ctgttattga atacaatcga tctat
//
LOCUS       DAXFZR010000010        37354 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000010 DAXFZR010000000
VERSION     DAXFZR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37354
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.010"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            69..1193
                     /locus_tag="ACFJAS_000395"
     CDS             69..1193
                     /locus_tag="ACFJAS_000395"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3981739.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
     gene            1190..2227
                     /locus_tag="ACFJAS_000396"
     CDS             1190..2227
                     /locus_tag="ACFJAS_000396"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HFC3981740.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            2243..4117
                     /locus_tag="ACFJAS_000397"
     CDS             2243..4117
                     /locus_tag="ACFJAS_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HFC3981741.1"
                     /translation="MNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLGNVQSG
                     KTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEADDRSFM
                     EVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTNSDKPA
                     KDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGEKYIGG
                     NFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKANCNFA
                     LHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIHHFDEI
                     YEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTVYYSRT
                     AKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKNSGGNI
                     QVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKDEIQSD
                     LYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGRKLSRA
                     TGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLVYQSAK
                     "
     gene            complement(4180..5244)
                     /gene="hemE"
                     /locus_tag="ACFJAS_000398"
     CDS             complement(4180..5244)
                     /gene="hemE"
                     /locus_tag="ACFJAS_000398"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC3981742.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFAHA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(5319..6536)
                     /locus_tag="ACFJAS_000399"
     CDS             complement(5319..6536)
                     /locus_tag="ACFJAS_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC3981743.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVFVVVGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPENMRRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            complement(6533..7867)
                     /locus_tag="ACFJAS_000400"
     CDS             complement(6533..7867)
                     /locus_tag="ACFJAS_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC3981744.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            complement(7879..8643)
                     /locus_tag="ACFJAS_000401"
     CDS             complement(7879..8643)
                     /locus_tag="ACFJAS_000401"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC3981745.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPAAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            complement(8661..9083)
                     /locus_tag="ACFJAS_000402"
     CDS             complement(8661..9083)
                     /locus_tag="ACFJAS_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC3981746.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            complement(9346..10314)
                     /locus_tag="ACFJAS_000403"
     CDS             complement(9346..10314)
                     /locus_tag="ACFJAS_000403"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3981747.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKEPLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            complement(10314..11138)
                     /locus_tag="ACFJAS_000404"
     CDS             complement(10314..11138)
                     /locus_tag="ACFJAS_000404"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC3981748.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            complement(11168..11809)
                     /locus_tag="ACFJAS_000405"
                     /pseudo
     CDS             complement(11168..11809)
                     /locus_tag="ACFJAS_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(11978..12298)
                     /locus_tag="ACFJAS_000406"
     CDS             complement(11978..12298)
                     /locus_tag="ACFJAS_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC3981749.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            complement(12321..12947)
                     /gene="upp"
                     /locus_tag="ACFJAS_000407"
     CDS             complement(12321..12947)
                     /gene="upp"
                     /locus_tag="ACFJAS_000407"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC3981750.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(13053..13364)
                     /gene="grxD"
                     /locus_tag="ACFJAS_000408"
     CDS             complement(13053..13364)
                     /gene="grxD"
                     /locus_tag="ACFJAS_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC3981751.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(13465..13680)
                     /locus_tag="ACFJAS_000409"
     CDS             complement(13465..13680)
                     /locus_tag="ACFJAS_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC3981752.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            complement(13706..14485)
                     /locus_tag="ACFJAS_000410"
     CDS             complement(13706..14485)
                     /locus_tag="ACFJAS_000410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC3981753.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(14490..14840)
                     /locus_tag="ACFJAS_000411"
     CDS             complement(14490..14840)
                     /locus_tag="ACFJAS_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC3981754.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            complement(14844..15821)
                     /locus_tag="ACFJAS_000412"
     CDS             complement(14844..15821)
                     /locus_tag="ACFJAS_000412"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC3981755.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            complement(15855..16985)
                     /locus_tag="ACFJAS_000413"
     CDS             complement(15855..16985)
                     /locus_tag="ACFJAS_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC3981756.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            17153..17854
                     /locus_tag="ACFJAS_000414"
     CDS             17153..17854
                     /locus_tag="ACFJAS_000414"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC3981757.1"
                     /translation="MSLKTVAVIGAMEQEIGLLREMMENVKAVSFGRFSAYEGELAGK
                     RIVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            17996..19789
                     /gene="lepA"
                     /locus_tag="ACFJAS_000415"
     CDS             17996..19789
                     /gene="lepA"
                     /locus_tag="ACFJAS_000415"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC3981758.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            19935..20954
                     /gene="lepB"
                     /locus_tag="ACFJAS_000416"
     CDS             19935..20954
                     /gene="lepB"
                     /locus_tag="ACFJAS_000416"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC3981759.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRAPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(21059..21166)
                     /locus_tag="ACFJAS_000417"
                     /pseudo
     CDS             complement(21059..21166)
                     /locus_tag="ACFJAS_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(21390..21539)
                     /locus_tag="ACFJAS_000418"
     CDS             complement(21390..21539)
                     /locus_tag="ACFJAS_000418"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981760.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(21585..22492)
                     /locus_tag="ACFJAS_000419"
                     /pseudo
     CDS             complement(21585..22492)
                     /locus_tag="ACFJAS_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(22801..23733)
                     /gene="cysK"
                     /locus_tag="ACFJAS_000420"
     CDS             complement(22801..23733)
                     /gene="cysK"
                     /locus_tag="ACFJAS_000420"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC3981761.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTEIYDSITKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(23929..24534)
                     /locus_tag="ACFJAS_000421"
     CDS             complement(23929..24534)
                     /locus_tag="ACFJAS_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981762.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            complement(24531..25382)
                     /gene="dapF"
                     /locus_tag="ACFJAS_000422"
     CDS             complement(24531..25382)
                     /gene="dapF"
                     /locus_tag="ACFJAS_000422"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC3981763.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            25519..25833
                     /locus_tag="ACFJAS_000423"
     CDS             25519..25833
                     /locus_tag="ACFJAS_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981764.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNTLVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            25830..26498
                     /locus_tag="ACFJAS_000424"
     CDS             25830..26498
                     /locus_tag="ACFJAS_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC3981765.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(26571..28694)
                     /gene="pnp"
                     /locus_tag="ACFJAS_000425"
     CDS             complement(26571..28694)
                     /gene="pnp"
                     /locus_tag="ACFJAS_000425"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC3981766.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKRLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(28928..29791)
                     /locus_tag="ACFJAS_000426"
     CDS             complement(28928..29791)
                     /locus_tag="ACFJAS_000426"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC3981767.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPI
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYEVGINPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            29984..30847
                     /gene="rfbD"
                     /locus_tag="ACFJAS_000427"
     CDS             29984..30847
                     /gene="rfbD"
                     /locus_tag="ACFJAS_000427"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC3981768.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(31219..31452)
                     /locus_tag="ACFJAS_000428"
     CDS             complement(31219..31452)
                     /locus_tag="ACFJAS_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC3981769.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(31470..31604)
                     /locus_tag="ACFJAS_000429"
     CDS             complement(31470..31604)
                     /locus_tag="ACFJAS_000429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981770.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLEDIVFSQIDHDSAASKALACAN
                     "
     gene            complement(31618..31818)
                     /locus_tag="ACFJAS_000430"
     CDS             complement(31618..31818)
                     /locus_tag="ACFJAS_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC3981771.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(31990..32865)
                     /gene="xerD"
                     /locus_tag="ACFJAS_000431"
     CDS             complement(31990..32865)
                     /gene="xerD"
                     /locus_tag="ACFJAS_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC3981772.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            complement(32917..33357)
                     /locus_tag="ACFJAS_000432"
     CDS             complement(32917..33357)
                     /locus_tag="ACFJAS_000432"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC3981773.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            33678..34616
                     /locus_tag="ACFJAS_000433"
     CDS             33678..34616
                     /locus_tag="ACFJAS_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC3981774.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            34745..35038
                     /gene="hfq"
                     /locus_tag="ACFJAS_000434"
     CDS             34745..35038
                     /gene="hfq"
                     /locus_tag="ACFJAS_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC3981775.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            35123..35689
                     /locus_tag="ACFJAS_000435"
                     /pseudo
     CDS             35123..35689
                     /locus_tag="ACFJAS_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            35743..36117
                     /locus_tag="ACFJAS_000436"
     CDS             35743..36117
                     /locus_tag="ACFJAS_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC3981776.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            36127..36621
                     /gene="folK"
                     /locus_tag="ACFJAS_000437"
     CDS             36127..36621
                     /gene="folK"
                     /locus_tag="ACFJAS_000437"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC3981777.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(36750..37229)
                     /locus_tag="ACFJAS_000438"
     CDS             complement(36750..37229)
                     /locus_tag="ACFJAS_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC3981778.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
BASE COUNT         9471 a         9858 c         8684 g         9341 t
ORIGIN      
        1 agagcatagt tcacctttaa aatccataca aggattttta tcaaatttat gcataggagt
       61 gaaatcctat gctatctaaa caaatctcaa atcttaattc ttctagtaac aaaccaaaaa
      121 tcctatctct attttcagga tgtggcggtt tggatttggg ctttcatcaa gctggttgtg
      181 aaactgtttg ggcgaacgat ttctcccatt gggcttgcga aagtttccgt aaaaatatcg
      241 gcgatgtcat cgtagaaggt gatattgaac aaattaatcc gaatgatcca actattcccg
      301 attgcgacat cattttaggc ggattccctt gtcaagattt ttccatgatt tggaaacagc
      361 cgggcttaga gggtgagcgc ggcaatcttt ataaaagctt tttacgtttt gtaaatgcaa
      421 aaaaaccgaa agtttttgtt gctgagaatg tgaaaggttt attgactgcc aacaagaaaa
      481 aagccatcca gcaaattatt accgactttg aaaattgcgg ttattacgtt caggcgaagc
      541 tgtataactt tgcagaattt ggcgtacctc aatttcgtga acgtgtgctg attgtcggag
      601 tacgtttgga tacaggattt gattttcgcc atccggaacc gacgcacaat gaaactggcg
      661 aaaacggctt aaaaccatat gtaacagcag gtcaggccat atccaatatt ccacaaaatg
      721 ccagtaataa tgaattactg aaaatcagcg ataaaacacg ccgtatgttg gaattaattc
      781 ctgaaggtgg aaattttacc gatattccta aagatcatcc tttatatgtg aaaggtatga
      841 ttagccacgt ttatcgtcgt atgcatcgga acgagccatc aaaaacaatt attgcagcag
      901 gtggcggtgg tacttggggc tatcacttcc ctgaaccgcg tgcttttact aatagagaac
      961 gagcaaggct tcaaagtttt cctgatgatt ttgagtttgt cggatcaaca actgaagtac
     1021 gtcgccagat tggtaatgct gttcctcctc agggcgtggt tgaactggca aaaagcattt
     1081 taccgatttt ttcagacaac tatgagaaag tagatttgca tgagaaatta gtcgaagaaa
     1141 aagaaatttt attccatgac cgactaagca aaattcgagg gggaaaacaa tgaatacagt
     1201 tttttccaat atcgccaatg ccaaaatcac tgaaaaatct cttaatgcgg tttggatgga
     1261 tttatttaaa tcagcggatg aagtgttgat ggcgacaggt tatgtatcta atgacgcagt
     1321 ggttgagtta cataaaattt tggagctaaa cgatcatatt caaaaaatag atttattggt
     1381 cggaatgcat tatttggaag gttttagcca tttacaatat gacagcttat gtaaactaaa
     1441 tgattttttg cgacatgaga aaagaggagc cgtctatgtt tccccctttg tgaaatttca
     1501 cggcaaaatg tactccttta aaaattatca aaaaatcaat ggattaattg gttctgctaa
     1561 cttaacgtgt ttttgggata gcacggaacg tacttatgaa actatgttgc atttgaacgg
     1621 taagcctgca cagattttgc aggcagatat tcaaagtact attcataagc taggcaaaaa
     1681 tatccaagaa gttgaaagac caagtaaatt tattgaacat aatagccatt tagaaaattg
     1741 tttgggtgtt cagaaaattg caccagaaca gattcggcaa ttatttgccc aaacgtcaga
     1801 atatcatttc tcaattccag ctaaaaccga agaaaaaagt aatttgaatg tattttttgg
     1861 agaaggtcga cgagataaac gagggtttgt caaaccacga ccttggtatg aagtagagtt
     1921 gattgtatcc aaagacatca cttcacaaga agggtatccg gttctgaagt cttttacagt
     1981 tattaccgat gatggttggc aatttcaatg caagacatcg ggggattact ccaaaaactt
     2041 ccgctcagaa aatgatttaa agacacttgg taaatggatt aaaggtcgtt tggaaagtca
     2101 tggctgcctg caaaataacg agaagattac gcatgaaacc ctacgcgaat acggtaacga
     2161 tcattttgag ttacgttcaa ccgataatcc tgatgtttgg ctgctctcat ttaaggggaa
     2221 aaactaaatg ttgcgaacct acttgaacca attgacccct cccgaattag ctgattctgt
     2281 taaaaatacc gttgatggtt ttatggaaaa attgtcacaa accgagccga aaatcgccca
     2341 aaatgtttta ttgctgggca atgttcaaag tggcaaaacg gcacaggttt tgggtgtatt
     2401 aagtgcgctg gctgatgatg gcgaccacaa agtttttttg tatcttacta ctgacagcgt
     2461 agatttacag gatcaaacag tcaaacgagc caaagccaat ctgaaaaact ttatcgtatt
     2521 gtctgaagct gacgaccgaa gttttatgga agtaatgaag gcagagaacc ccatcttggt
     2581 cgtgattaaa aagaatgccc gtgtattgaa acgttggcgt aatttgtttg ccagtcaaag
     2641 tagcctgaaa ggctatcctt tggttattgt ggacgatgaa gccgatgctg ccagcctaaa
     2701 tactaattct gataagccgg ctaaagatgc cagtactatc aataaactgc taaacgacat
     2761 caaaaattcc tgttgtcaaa gcctgtttat ccagttgact gccacgcctc aatcgctttt
     2821 attacaacat gaagaatctg attggcaacc tgaatttatc cacttctttg aagcaggcga
     2881 aaaatacatc ggcggcaact ttgtcttttc tgatccacca agctatatag ttcgttttat
     2941 tgatagcgaa ttggatgata tgaaagacga aagtggtgaa attgccgaag gagcaaaaca
     3001 ggcattgctt agttttctaa taacctgtgc agaattcgcg ctatgtgata aggctaactg
     3061 taattttgca ctacatccaa gctataaaat tcaagatcat caagcctttt caaagaagat
     3121 ccaagccttt ttaaatgact tagtgcaagc agtcaataat ggggaagacc ttgcaggtag
     3181 ctttaaagaa agttatttgg atttgcaaaa gaccaagccc gatattcatc actttgatga
     3241 aatatacgaa aaattgacag cacttttaga gaataaacaa atctctactc ttgtcgttaa
     3301 ctcgcagaca gaaacagatt ttgacttaga aaaaggtttc aacatcatta ttggtggtaa
     3361 tgtgattggt cgtggcttga ctattccgaa actacaaacg gtttattata gtcgcactgc
     3421 caaaaaaccg aatgcagata ctttctggca acattcgcgt atttttggat atgaccgcga
     3481 taaatcatta ttacggcttt atattccgtt cgatgtctat tacttctttg tacaactcaa
     3541 tcaagcaaat aatctgatta ttgggcaggc aaaaaattca ggcggcaata ttcaggttat
     3601 ttatccgaaa aatatcaatc caactcgaaa aaatgtattg aaattcgata gtatcaatca
     3661 aattgtgggt ggtgtgaatt attttccact ccacccaaac gaagataatt tatcggagat
     3721 taacaaaatt ttgccttcta ttttgaaaga tgaaattcaa tctgatttat atcaaataga
     3781 tatagaagat ttattcttgg ttctagataa attgggtcgt tatgttccag atgactggaa
     3841 taaggaaaaa tttattgctg gtgtagaagc attaaaagca caacgtccaa gttttaaaac
     3901 ctatgttttg atcaaaaccg gacgtaaact ttctcgagca accggcacaa tgctttcgga
     3961 agacgaccgt aaattaggag aaaaatatcc caatgattta ttccttacgc tttatcaagt
     4021 agtaggaaat aaagacaaag gttggcaggg aaaagatttt tggttaccca atatcaagct
     4081 accacacaat ggcttggtgt accaaagtgc aaaatgaagc atatgccgtc tgaaaacttc
     4141 cgattcttca gacggcatat cgggcggttt cctgccggct tacccgccgt gatactgccg
     4201 cgacagctcg tgtacggtgt cgactaagat tttggcgtgt tcggggtcgg cgtgttggtt
     4261 gatgccgtgt ccgaggttga agacatggcc gctgccgtgt ccgtagccgg tcaggatacg
     4321 tgcgacctcg gtgcggatgg attcgggcgt accgaagagg gcggacgggt cgaagttgcc
     4381 ttgcagggcg acttggttgc cgacgcggcg gcgtgcttcg ccgatgttgc acgtccagtc
     4441 caagcccaat gcgtctgcgc cgatttgggc catgctttcc agccacagcc cgccgccttt
     4501 ggcaaacacg ataacaggca cgcggcgacc ttcgctttca cgtttgagtc cggcaacgat
     4561 ttggcggatg tatttgaggc tgaactcttt aaacgccgcg tcgctcaaca cgccgcccca
     4621 agtgtcgaaa atctgcaccg cctgcgcgcc cgcgtcgatt tgggcgttga ggtaggcggt
     4681 aacggcttgg gcgttggtat cgaggatttt gtacagcaaa tcggggcgcg agtacatcat
     4741 ggttttgatg gtgcggaatt ctttgctgcc gccgccttcg accatataac aggcgagcgt
     4801 gaacgggctg ccggagaagc cgataagcgg tacgcggccg tccaatgctt tacggatgga
     4861 agttaccgcg tcgaaaacgt attgcagttt ttccatgtcg ggaacgtgca gcttggcgat
     4921 gtcggattcg tgttgcaagg cgtgtgcgaa tttcgggcct tcgccttcgg caaaatacag
     4981 ccccaagccc attgcatcgg gaacggtcaa gatgtcggaa aacaaaatcg ccgcgtccaa
     5041 atcgaagcgt tccaaaggct ggatggtgac ttcggtcgcc aatccggtgt ttttgcacaa
     5101 atcgaggaag ctgcccgctt tcgcgcgtgt ggctttgtat tcgggcagat aacgccccgc
     5161 ctggcgcatc atccaaatcg gtgtgtattc gacgggctgt ttgagcaggg cgcggaggaa
     5221 ggtatcgttt tttaaaagag tcatacgggt tttctgtcag tctgtttgtc aagtgaaatg
     5281 gttttcagac ggcatttcaa cgatgccgtc tgaaggtttc aacgggtttc ggcggaaggg
     5341 cggttttccc cggcaacgct tgccaaaacc aagttgcgct gtgcttcggc tttttgcgac
     5401 tgtgcggttt cgtcaaaaac ctttgccaac accaaacgcg ccggaatact cggcttcagt
     5461 gcaatactcg cttcaaggta gccttttgcc ttaccccaaa gtttgcggcc gtaggcgagc
     5521 cggccgagat acatcagcag aagcgcgtta tcgggctgtt ctttcagcca agaatcggca
     5581 aaatcgatgg ctttctgctg ttcgcgctcg cccaaaaagc gcacgctttc gacaaaggct
     5641 tccaaaagct cggggcggcg gttgtgcgga taatgctgtt tgacccattt gaccgcatcg
     5701 gcatacagtc ccaaacgttc gtacttttcc gcaaccgata cgctcaattc cccgtttttg
     5761 aggctgtcgg gaatccgctt caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc
     5821 atctggcggc ggtatgccca attttgatac cgttccattt ccgatttgcc caacgcgccc
     5881 gccttggaaa gtttttcggt ttttgccaga acctgcaacg catcgccccg atcgaaggcg
     5941 taacgaagtt gcagacgcac gaggcgcgta aggttggcat tcatcttcgc cgccgcatga
     6001 agattggctt ccgccgcttc gtaatcgcgc cggttcagtg ccgattccgc cagcagcagg
     6061 tagcgggaaa gctgctgttt ttccggcagt ttggcgattt ccgcaagata acggtcgcgc
     6121 agctcgatgt tttccatctg tcctgccgcg tgcgcgccca gcatcaatgc caaagtccgg
     6181 ttgtctccgg cctctttgtt gcccaacacg cgagaggctt cgagttccgc cttttcaaaa
     6241 cgcccttcga aatacgccaa acctgcctta ttcaaggcaa gcgcggcctt gcggccttta
     6301 cgcgcagaac cggaacgccg catattttcg gggatattga gtacgccgat gatgaattta
     6361 aacaggaaat accacaccac gacggcaatc agcgaaccta acacaaaggc gtgcaggttg
     6421 attctgagca tggtctgccc gacgacgacg aacacgtcgc cggtgtaaat gcccgaagcc
     6481 agcgccagtc cgacggcggc ggcaaacagg acgacaatcc agactaccgt tttcatgcgc
     6541 gttctccttt agcggacggc gaacgccctg ccttttccgg tgcgggctgt ttttttgcag
     6601 gctgttccgg tttgcgttcc gaaggcaggg acggtgcttc tatggccttc acatccgatg
     6661 cggcagccgg ttcggaagct gtgttttcgt ttgccggttc ggaagccgcc tgttccgctt
     6721 cttgattttc cgccgccgtc atgcgcgtac cgtcgcgata ggcgcggacg gcatttaaac
     6781 tggtcttcaa gccgtcatcc gccgtcatgc gtacatccag tgttttcaat tccgccagtt
     6841 ctttcagcca cgactgcgtg gcgggcgact tggcatcgaa atactgtctg acggcggctt
     6901 cggcattgtt caaatcgccc tgatagactt cgccgttgcg ctgcatcaat gcggtgcgcg
     6961 catccaacag gcgcagacgc aggttttcac gcacaaaata tgcctgttcg ggagaaatca
     7021 gcatggcatc gttgttttcc aaacggcgga tttcgaccag ccccttcaat gtgccgaggg
     7081 atttttccca tacgttctgc caccatgaag cggaaacggc ttcgttgcgg gcctgtacgc
     7141 ccggtttcaa tacgccgtcc aaaatcagcg gcagtccgga tacggcggtt tccaaccggt
     7201 cgaggcgcaa tgccgtgccg gaaatatcga catagggacg gtttttcagt tccgccaaat
     7261 cgctgctgac cgcctgtttg atcggcagaa gctctgcctg atcgaaacgg gacaggcggc
     7321 tgtcgatatg ctccaacacg ccgactgccg tttggatatt gccggtcaac accagctgtt
     7381 gcgccgccag attgagtatg gtctcggttt cgtccaccag ccaatcggca cgccctttgg
     7441 tcagctcgcg ataggctttt tgcgtcatca ggatttgttc gccgttggct ttgacaccgc
     7501 tgtccaaacg gtcgagttcg gactgtatgg cactttggcg gttgaggttg tctttgagca
     7561 gggcggcgtt ttccgactcg cccaaggcgg ctttgtcgat tttctggttg aatgccagct
     7621 cttggttttt taagacattc tgtccttgga caaacaaaaa accacttgca cccaaaccca
     7681 atgccgccaa taccaaggcg caggccgcca aagcgttgct gccggactgt ttgacgataa
     7741 aaggagcggc gggaatgtgt acttctgttt cgttttcttt gcgtggggaa gaggtttcag
     7801 acggcatttc ttttgatgcc tgtatctcgc gtacgggttc ggatgatttg ttttcaggtt
     7861 cgcccaccgt tttgctcctt attggtttga ggttccggga aagaccatgc cgtctgaaac
     7921 ggaaatggaa gaataggaaa gcgcggcttc cagcgtaggg acggtttcga ccgaacacac
     7981 gccttcgcgc ttcaatgcct ccgcaatgcg cggatgatgg gtaaagtata gcaaggattt
     8041 gaagaatcgg gaaaattgcg gcggaagctg tgcaaacagc gactgcacaa gttctgtgga
     8101 cgtaatatag gcggcggcaa tattttcggt ttggaaattt tgaaagttca aaggtttatg
     8161 ccgtctgaaa tagacttctg ccacctccgt ccgaaaacct ttctcctgca aggcattcat
     8221 cagaaaatcc cgcccgccgt gtccgcgcac agacaatacg cgcgcacctt cgggcagact
     8281 gttccaaacc ggcaggcgca aaaccgcctc gctgtcgttg ccgtcttcag gtgcaaacac
     8341 gcccgcgccc gcataacgcg ccaaggcacg gcggctgcct tgccctacgg caatgtgcgc
     8401 ctttatgccg tctgaaaggt taaggtacgg gacggcggtt tcaacggcgg ccggactgac
     8461 ccaaaacacg gcatccgcac gggcatacat ttccggcagc cgtttcaggc ttgcttcgtc
     8521 ggtttcgatt tcaatcggac tcaacacttc cgcctgccat ccggcacgac ggcagacttc
     8581 cacatcgtcc ttcgccctgc ccgacgggcg gacaatcagc atgacgggct tcgctgtttc
     8641 catcgcctta tccgccccga ttaccgggaa aaatcgtcat cgttgaaatt cgcctgcgcg
     8701 gttttcggcg tacagcctac ccgtgcgact tccttgcccc tacgctccac aacgacaccg
     8761 ctgctttccg tatgagtcac ggaatcgaag cctgtccata cggtgtacat aaattcgccg
     8821 ttttggaatt tcatcgttgc ccggcgtccg ctgcccatgc cttgccacct gtcggaacgc
     8881 cccaacaggt cagctttgct gttgcgtatg gcaatttctt tttttgccgc actgccgaac
     8941 gaatattcgt aaagattgcg gttgattttt tgaacttcta tgtatttgtt gttgtccgtt
     9001 ttacaggaaa ataccgtatc cgcccacacg gtttgggcgg cagccgacag aagcaatgcg
     9061 gcagaaagga acattttttt catcataatt tccttaatgg ttgaaacccc gccattcgga
     9121 catccgtcct tcgtggcagc agcatcagat tttatttgaa aggggtgtaa cccctttcaa
     9181 attagggcaa cacatagggc ggcgctttat gtgtcgtcct gtgtgttgaa acatcattgc
     9241 ccgtatcctg aaatagaagg ctgtgtttta acaatccgtt gattgcttaa gatgaattca
     9301 cactaaaaaa cagttctcta tttcatctat tacgagggtg gtaatctaca aattgttact
     9361 aaatagcaag gtgtgaatga aacggcagat ataatcaaaa tcttcttgtt ggtcaaaata
     9421 cggtaacata atgcttttta atttttcgcc tttgggttta cgtccataag aaaacagata
     9481 gcggtttgct gtaatacatt tagcgtaata cagttttact tctattggca tatcggactg
     9541 tatgggtgtt aatacggcga catcactatt tgctacaaat tcacaagttg aaacatagct
     9601 ataagtatgg ctaccttcgc cattggtaga agtgaaaagc gtatggcatg gaaaaatatg
     9661 tttttcatca acgctatcac gagcgatata acttcgtagc gttcttgctt gcgtactggc
     9721 aggacggata tagacaaccg tgtctttttg ccgttgttca aattctttta atttggttgc
     9781 ggcaatgccg ttttgaatag taaaaatttc atcaagtcgt tttgattgtc gaaccacagg
     9841 caaatcaagc ggttctttta atttcggttc ggttacaccc gcagatggat ttaaattcac
     9901 gctttcaacc cagcttggga tctcatccaa agatggcaca aacagttgtc gtaacgtgcg
     9961 gttagcttcc ctaccgcaag cagaaaaacg gtaggcattg gcaaaaatag cagcacaata
    10021 aaagagtttt tgttgttctg tcatttctgt tttgggattt aaaacagcaa cattttgagc
    10081 tgtataaaat ggtttgtttt gtaaaaaagt agataaaaca gaaccaccta atgcaacagt
    10141 taataatcct gctggataag gttttgtatt tttcaataaa tccacagatg ctgttacacc
    10201 attatttttg cctgacctgc ctacaaagtt gattgttgga ttgaagctgc tcattttatt
    10261 caaatctaat tttgaaccgt aactcacatc aaaaatctct tgcaattcaa ccattattca
    10321 tcgtctccga agtcgttatt gtcatcgtct tcatagcttt cattatccaa attttcagtc
    10381 ggccctactt ccgctcccag tagttgaaat aaagcaaatt cacgcacgac ttgctcaaaa
    10441 tctgctcggg taattttgga ataatcagtt tccaaatagg cttcggcaac ccattcatcg
    10501 ttatcagtta caaatgctgt taccgattct cctgtgtgaa cttcgttgtt taaataatgt
    10561 tccaaccagc gcgctttaat gccctgccag cggtcgtata aatcaatgcg tcccaagtgt
    10621 ttggtttttt caaaaccgtc atcacgccag tagccaaacc acgttttgcg acaggctttt
    10681 ttcgtgctga tttcttcttg tgtgtccggg tcaatcacga ctgtctgaaa atgcggttta
    10741 tgggcttcaa atacgacaat gcacgttacc gtgccaactg ggtaaaacag ttcgctcggc
    10801 atagacatca cggcttttaa gcgatgatat ttcacaattt cgcttttggc tttgcttggt
    10861 gcaatcacgc aactgactgg aataatggca atgcctgtgc caccttctgc caacatatcc
    10921 aacatttctt tgacaaaata aagttcatgc aattcggcat cgcttttgga ttgggcgtaa
    10981 ggtggattga gaaagccaac atttgggcgt tttaagcctg tttcaggttt gggatttttg
    11041 attaaatcct gaaaatcagt catgaaacaa ctgctttggt gtaaattggc tttaccgtct
    11101 ccgcgtaaaa tcatattact ggcggcaaga gcaaacattt ttgggttgtt ttcaatgcca
    11161 atcagttgat attttttaga gatggcttac ccaaattgct aattctagca acagatttac
    11221 cgtctaaatt gataccacaa ggaacacaaa cttgttcttt ggaaatgcca cgtttcgttg
    11281 ctctcgttcc tcgtttgtga gcaggacgtg gcagattaaa atttttgtgg tgccctttgt
    11341 aagaaatcgt cgaataagtt tcatcagctt gcacaatgcc gtctaactca acttcattca
    11401 tcatgttttg aagtgcgtcc aaaattttgt gccgccaagt aaacgcagta gcaagattga
    11461 ttttgcaaat ttcagcacat ttgcgaagtg ggtatttttc aatcatacaa tgaatgtatt
    11521 tttcccaaac ttcaatgtct ttttgcgtgt tgtagagaat agtgcctgtc tgctcaacaa
    11581 aagacttttt acaatcacga cacaaaaaac gttggttgcc acaatctttg ccatttttga
    11641 cgaaatgtgt agattggcaa tgtgggcact tcgtaacttt tcttggttca atgacttttt
    11701 taacctgttc ttttgagctg acagaagtca aaaaagcctg tttatccgta tcggacaaat
    11761 tttcaaaaag ttgttttaaa atgtctaatt cttttgttac gcccgacatt ataaaatccc
    11821 ttatagttca atttgttata agggatttta tcaaaaatta gtgcaaaaat caacggattg
    11881 ttaaaacaca gccaaataga aaagaaaatg cccgttttac ggtcttcttt ccgacaaaat
    11941 gccgtctgaa acgaatttca gacggcatgg tcgaaaatta ttgcgcggca agcggtttga
    12001 gcaggagggt ttcgccgctt tgcacgacat tttccagcaa atcgatgttc ggatgcttcc
    12061 ccggatgggc gcgcgcgtcg gcaacatgtt cggcaaacca tatcaaaccc tgttctgccg
    12121 cttctttacc caattttcca tcaaaacgtt ccgccaaggc attgtacagc ttgagcgaac
    12181 cgagcttgcc ctgaacggcg ggaatgtggt gaaccgtttt gccttcggca tcctgaacat
    12241 ccaaaccgcc cagatggtcg attgaaggaa atgtggcgag atagtcttgg aaattcatat
    12301 caaatccgaa aatcagtcag ttagcgcgtg ccgaaaatct tgtcgcccgc atcgcccaga
    12361 ccgggaataa tatagccgtt ttcgttcaaa cggctatcca gcgcggcggt ataaatcgta
    12421 acatcgggat gcgcatcatt aacagccttc acgccctcag gggcggcaac caagactaag
    12481 gctttgatgt tgcggcaacc tttttctttc aacaggtcga tggtggccac catcgaaccg
    12541 cctgtcgcca gcataggatc gataatcaaa gccggacgct cgtccatact gtccacaaat
    12601 ttctcaaaat aggaaatagg cttcagcgtt tcctcgtcgc gctgcaaacc gaccacgctg
    12661 attttggcag tcggaatcag gtcgagcacg ccgtcgagca tacccaaacc ggcgcgcagg
    12721 atggggacga cggtcagggt tttgcctttg atgcggtcgc cttcaatctg tccgcaccat
    12781 ccgtcgataa ggtatttttc aatttcaaaa tcacggcttg cctcgtatgc catcaggcgc
    12841 gccaactcgg tggtcagcgt acggaatttg taggtgctgc aatccgcctc cctcatcagg
    12901 gtcagtttgt ggcggacgag cggatggttg ataacgttaa cgttcatcgc ggtgttccag
    12961 tgtgattttg agacgcgtca ttatactttt ttttaatttg ccggaaaaga aaatgccgtc
    13021 tgaaacacgt tcagacggca ttgccgaatc catcaggctt tcagcagctc ttgcagctcg
    13081 cctgcctcat acatttccat caggatgtcc gaaccgccga caaactcgcc gttcacataa
    13141 agttggggga tggtcggcca gtcgctgtat tccttaatgc cttggcgtac ttcgggattt
    13201 tccaatacgt tgacagcaac gtaatcggtg cagcctgccg cgttcaggat ttgcacggcg
    13261 cgggaagaga aaccgcattg cggaaactgc ttcgtacctt tcataaacaa tacgacgcgg
    13321 tgtgtcgtta ctacttcttt aatttggtcg tggatggaag ccatcgctta ttccttttga
    13381 taaagttgac aaaatttgtc aggtagggcg cattatacgc aaaagcggaa acttcccaca
    13441 atatttgccg tatatcgcgc agtatcaatg ttgcgaacgg caatattccc actcctcgac
    13501 aatcctgctg tccggcaaat ggcacagggc atatccgccg ccgctttcgt ctttttttac
    13561 ttcccgtctg ccgcctttgg caatgcagaa tttggaagcg gaatttgcca tgcctgtccg
    13621 aaacgtgttg gcaggcagcc ggcagcagcg cggacatgca tacggatgaa ttgccttcat
    13681 ggttttcctt atcgtcggca cagggtcaga cgacccgcat atcgggtact tttttaaaca
    13741 gccggtaaaa gttttccgtc gtatatgccg caacctgttc caatgtttga ttccgcaatt
    13801 tggcaatatg ttcggcagta tgacgcacaa aagccggttc gttctgcctg cctcgtttgg
    13861 gaacgggggc aaggaacggc gcatcggttt ccactaaaat gcggtcgtcc ggaacgtatt
    13921 tcgccgcctc ctgaaccaag ggggcgtttt taaaggtaac gattcccgag aaagaaatat
    13981 aaagccccaa atccatggca gcccgggcaa aaccgacatc ctcggaaaaa cagtgaatca
    14041 cacccgaatt ggttttacat tcttttaaaa tcgccaaggt gtccgccgcc gcatcgcgcg
    14101 tatggacgat aacgggcagt ccggtttgat tggctgcttc gatgtggtct gcaaagcgtt
    14161 tgtgctgcca agacaaatcg cctttgcacc agtaataatc caaacccgtc tcgccgatgc
    14221 cgaccacttt cggatgggcg gcggcttcga ccatttccgc aatggaaaat tcttcggctt
    14281 ccttgctgtc gggatgtacg cctatggtgc aatagatgtg ttcgtgcgct tcggcgacgg
    14341 caaagacttc ggagaagctt tccctactga cgctgatggc gagtgcctgc cccacgccgt
    14401 ttgcttccat gttggacaaa acttcgggca ggcgttcttt caaaccttca aaattgaggt
    14461 ggcagtgcga atcgatgata tgcatagcgt tacatggtaa acgtaggtct gctgccgctg
    14521 attgtgctgc ccagttcgac ttcgatctgg tctttgactt tcaggcagtt ttcgtgtttt
    14581 gtgaatgcca gccccacccc tttgggtttg gaggaagtac tcgcaggatt aatccaggcg
    14641 accttggtcg gcaggaacag tttggggaag ttgaggattt ctacggcaag cagaatatcg
    14701 tccccgatgg aaaatacgtc gtcggtctgc acaaacagac cgccgtgttc caaaaacggc
    14761 atataggagc tgtacagcag attcatgtct ttcagctgca acgacatcat ttttgccgga
    14821 atattttgtc cgtctgacat aattcacctg ttctctttct tcaattccaa atagttgatg
    14881 agcagatgct cgatctgcat tttgacattt aaagtatgaa acccgtaagg ggcaagctgt
    14941 ttgagcatat cctccgccgc aaatacattg cgcggacgga aacccgatgc cgtctgaagc
    15001 agcctgtctt cataagcggg ataatagacg ggtttcatgt gttgcaggca caagcctaaa
    15061 tcgaccagcc atttctgcat ccacccgaca aatacggcga gcggaagttt ttccttatcg
    15121 aaaagcgcgg cgtaatccaa aatcttcaac aatctcggct ctgccaatat ctccagcagc
    15181 tttgccctca attcccgaag ctcgccttcc tcctgaaaca gcggcgcgcc cgaatggaaa
    15241 gccagacgtt cctcaggctc cgccacaccc ctgtcgcgca gatacgccaa tgcttcccca
    15301 tgcgaaggag cgggcaaaac cttcttccgg cagcggcttt taatggtcgg taaaaccttg
    15361 tccgccgcgt ggcttaccag caaaaagacc acttgtggcg gcggctcttc caacactttc
    15421 aacaaactgt tggcggcttg gacattcata ctttccgcag gatgaatcag gatcacgcgc
    15481 aaaccgcccc gtaccgaagt caggtacaca ttatcgatga tttccctgac ggcatcgatt
    15541 ttgatccgca acagtttgcg tccgttttcg ggttcgtccg ccagaggcgt gatttcgtaa
    15601 aaatcgggat ggcttccccg tccaaacaga tggcaggaca tacattcgcc acagggtttg
    15661 cagcccggtg caggggtttc gcacaacagc gccttcgccg caaagcgggc aaacgcagtc
    15721 ttccccgtcc cctttttgcc aacaaacagc cacgcattag gacggctcgt ccaatgctcg
    15781 gcaatctgcc gccattgttc ctgatgccac ggataaatca tgtcaaactg cccgtccgga
    15841 aatccatacc gccatcagag cagttccaaa tcggggtcgg aactttgtgc cctgcggctg
    15901 cgcaactgta ccgccaaacg caaatcatgt gcggaatcgg catttttcaa ggcatcctgc
    15961 aaggaaatct cgcctttttc atacaattgg taaaggtgtt ggtcgaaggt ctgcataccc
    16021 agggtagtgg attttttcat cacttctttg atttcatgga tgttgccgtt gtgaatcaac
    16081 tccgaaatca ggggcgaatt gagcagtacc tcgactgccg ccaccctgcc cttgccgccg
    16141 tctcgcggaa cgaggcgttg cgaaataaac gcctgaaggt tgagcgacaa atccgtcagc
    16201 aattgttcgc gccgctcctc ggggaagaag ttgatgatgc ggtcgagcgc ctgattggtg
    16261 ctgttggcgt gcagcgtcgc catacacaaa tgccccgttt cggcaaaggc gatggcgtag
    16321 tccattgttt cacggtcgcg gatttcgccg ataaggatca catccggcgc ctgacgcagc
    16381 gtatttttca acgccgccat ccagttttcc gtgtccacgc cgacctcgcg ctgggtaatg
    16441 atgcagtttt tgtgttcgtg gacaaactcg atcggatctt cgatggtgat gatgtgtccg
    16501 aacgaatttt cattgcggta gtcgataagc gaggcgagcg aagtcgattt gcccgagccg
    16561 gtgccgccga caaaaataac cagcccgcgt tttttcagcg caacatcctt caaggccggc
    16621 ggcaggttca ggctttcaaa cttgggaatc ttgctggtaa tcgcgcggaa taccaacgcc
    16681 gtcgcaccgc gctgtatcat cgcattgacg cggaagcggc tggtgtccgg caggctgatg
    16741 gcgaagttgc actcgttggt cgatgaaaat tcttccgcct gcttcgcact cataatcgaa
    16801 aaggcgattt ccatacattt ttccgccgtc agcggttcgt ccgtgatgcg ggtgattttg
    16861 ccgtccagct tcatagcggg cgggaaatgg gtcgtcacaa acaggtcgga acctttgttt
    16921 ttgttcatat ggcgcagcca tgcgaacagt tcttcctttg cagtcatatt ttcatcggta
    16981 aacatcctcg tcccccaatc ttcaaacaat ctgtaccttc ccattctacc aaaaatccgc
    17041 cgtgctgtct gaaaccgttt cagacggcac ggacaaaccg cctccactca atcctgccgt
    17101 gttagcgtgt ataattcggc gttctatccg aaattcacag caggttagca atatgtcttt
    17161 gaaaacagta gccgttatcg gcgcgatgga acaggaaatc gggcttttgc gcgagatgat
    17221 ggaaaatgtc aaagccgtct ctttcggcag attttccgcc tatgaaggcg aattggcggg
    17281 aaaacgcata gtgcttgcat tgagcggcat cggcaaggtc aacgcggcgg ttgcaacggc
    17341 ttggcttatc cgtcaattcg caccggactg cgtcatcaac accggcagcg caggcggttt
    17401 gggcaagggt ttgaaagtcg gcgacgtggt gatcggcacg gaaaccgagc accacgatgt
    17461 cgatgtaacc gcattcggct atgcccgggg gcaagtgccg caactgccgg caaggttcgc
    17521 ttcagacggc atattgattg aaacggcaaa acgggcggcg cggacgtttg aaggcgcgga
    17581 ggtagaacaa ggcctgattg tcagcggcga ccgctttgtc cacagcagcg aaggcgtggc
    17641 ggaaatccgc aagcacttcc ccgaagtcaa agcagtggaa atggaagcgg cggcgattgc
    17701 ccaaacctgt catcagttgg aaacgccgtt cgtcatcatc cgcgctgttt ccgattcggc
    17761 ggacgaaaag gcagacatca gctttgaaga gtttttgaaa acggcggcgg caagttctgc
    17821 aaaaatggtg gcagaaatcg tcaaatcttt ataaaacctt tatcaaatca tgccaaaaca
    17881 gcggttttcc gttagaatat cgggctattt ccacgttgcg cccgatattc ggacgtaccg
    17941 ctgtttgttc agacggcatt tctaaaacct accgttcagc attaaaaaca actcaatgaa
    18001 aaatatccga aatttctcca tcatcgccca catcgaccac ggcaaatcga cgcttgccga
    18061 ccgcttcatc caatattgcg gcggtttgga tttgcgcgaa atgagtacgc aggtgctcga
    18121 ttccatggac atcgaaaaag agcgcggcat caccatcaaa gcgcaaaccg ccgcactcaa
    18181 ctacaaagca cgcgacgggc aggtgtatca gcttaatttg attgacacgc cgggacacgt
    18241 cgacttctct tacgaagttt cccgttcgct gtcggcttgc gaaggcgcgc ttttggtcgt
    18301 tgacgcgtcg cagggcgtgg aagcgcaaac cgtggcgaac tgctataccg cgattgattt
    18361 gggcgtggaa gtcgtgcccg ttttgaacaa aatcgacctg cccgccgccg accccgagcg
    18421 tgtggaacag gaaatcgaag acatcatcgg catcgatgcc gtcggcgcgg tgcagtgttc
    18481 agccaaaagc ggcatcggcg tggaagacgt tttggaagaa atcgttgcca aaattcctgc
    18541 gccgaccggc gacgaaaacg cgccgctgca agccgttatc gtcgattcgt ggtttgacaa
    18601 ttacgtcggc gtggtcatgc tgattcgagt gaaaaacggc accatcaaac tgaaagacaa
    18661 agtgcgcttt atgagcacca aggcggaaac gcaggtcgag cagctgggcg tattcacacc
    18721 gaaatcggtt caaaaacaag aactaaaagc cggcgaagtg ggttttttga ttaccggcgt
    18781 gaaagaattg ggacaggcga aagtcggcga tacggttact ttggttgcca accctgccac
    18841 tgagccgctg cccggtttcc aagaagtaca aagccaagta tttgcgggtc tctatcctgt
    18901 ggaaagccac gattacgaag ccttgcgcga cgctttggaa aaattgcagc tgaacgatgc
    18961 ttcgttgaaa ttcgagcctg aagtttctca agcattgggc ttcggcttcc gctgcggctt
    19021 cttaggcctg ctgcatttag aaatcgtgca ggaacgcttg gaacgcgagt tcgacatgga
    19081 tttgattacc accgcgccga cggtggttta cgaagtcgtg ttgaaaagcg gcgaaaaaat
    19141 cgaagttgaa aacccgtcca aactgcccga catcggcagc atcgaaacca ttctcgagcc
    19201 gattattacc gcgaccatcc ttgtgccgca ggaatacgtc ggcaacgtca tgactttgtg
    19261 taaccaaaag cgcggcgtgc aggtcaatat gcagtatatg ggccgccaag tgatgctgac
    19321 ttacgacttg ccgatgaacg aagtcgtaat ggactttttc gacaaactca aatccacttc
    19381 gcgcggttat gcttcgttgg actaccattt caaagaattc caaccgtctg atttgattaa
    19441 gctcgacatt atggttaacg gcgaaaaagt cgatgcctta agcctgattg tacaccgtca
    19501 aagcgcggtt cacaaaggcc gcgagctggc atcgaaaatg cgcgaactga ttccgcgcca
    19561 aatgttcgac atcgccgtcc aagccgccat cggcagccgg attatcgccc gagaaaacgt
    19621 taaagccctg cgtaaaaacg ttttggcgaa atgttacggc ggcgatatta cgcgtaagaa
    19681 aaaactgctt gaaaaacaaa aagcaggcaa acgccgtatg aaacaagtgg gcaacgtgga
    19741 aattccgcaa agcgcgttct tggcgatttt gcaggtgagc gacaaataat atttcggcac
    19801 atagggcggg cgcgtcggga acacctctca aaccgccctg atttcagcgg cacgcgcccg
    19861 caaacaaatt ttgcaatttg ccgccttaaa acggttgcac ggccgggcgg gtttttaacc
    19921 gttaaggaac aacaatgaac acaatgctaa tgtcgggcgc ggctgccgcg ctgcttgccg
    19981 gcatcatcct ttatttcaaa agcgacaaga aacggcagga aaacggtgaa tggagttccg
    20041 gcctcgaata cgcctatatc ctgacggcgg tcggtgtgtt tgccgctttg tccctgttta
    20101 tgagctttac cgccgttttc ctgattttcg ttgtactgtg cggtacggct tggggggtat
    20161 ataaataccg cctgaagact catcccgaaa tctcggaaag cagccacttc ggcgattatt
    20221 tcggcagttt cttccctacc gttttggtat tgttcctcat ccggtcgttt attgccgaac
    20281 cgttccaaat cccgtccagc tcgatgcgcc cgggattaat caagggcgat ttcattttgg
    20341 tcggcaaatt ttcctacggc ctgcgcgcac ccgttttaaa caatgtattc atccccacgg
    20401 gcaaaatcga acggggcgat gtcgttgttt ttaactatcc tttgcagccg gagatgacct
    20461 acatcaagcg tattgtcggc atcccgggcg acattgtcga atatcgagat aaagttttga
    20521 cggtaaacgg aaaaccggct tccgacattc ctgacggcac ataccgttat cctgacgata
    20581 ccgacccatc cgaaatccac aacacggata tgttccgcag cggtctggac ggcaaatcct
    20641 tcaatattct gaaaaaagaa ggacagcctg ccgtttccct gcccgtattg ggcaaatata
    20701 cctccgatat tatgtctgaa aacggatatt ccatagatca aagcggtctg aaccattgcc
    20761 aatatgccga cgacggcagc ggtttcgtgt gcaaagttcc cgaaggacgc tatttcgcta
    20821 tgggcgacaa ccgcgacaac agtgccgatt cgcgctactg gggctttgtg gatgacaagc
    20881 tggttgtcgg caaggcaatg ttcattttga tgaacttcgg cgatttcggc agggccggca
    20941 cggcaatccg ttaagccgga aaattacgcc ggaacatcaa aaatgccgtc cgaaccctgt
    21001 ttcagacggc atttttatca tcaaactcct tgtttcccta ccggcagcct cggggataat
    21061 atagtggatt aaatctaaac cagtacagcc tggcctcgcc ttgccgtact atttgtactg
    21121 tccgcggctt cgtcgccttg gcctgatttg aatttaatcc actatatgca ccgccttgcc
    21181 ggcattatcg atatgggcgg cacatatgga ttgcctggaa aaatgatgcg ttcgggcggc
    21241 gtatgatttg cctgtgttta aatcatcggt tgattttatc cgtcggcaag tgaaaagccg
    21301 gttatttccg tttgtgtgga aacccatccc attgaataaa gggcatttcg tccgactgac
    21361 ggtgtgggcg gcatatggaa atacggacat tattttagtt gcattgccat tcctgcggag
    21421 gcggcttttt tgattctgcc cgccccgatg ccgtctgaaa gggcttcaga cggcattttc
    21481 cgctccgcgc cgttgtcggc atgtttggtc ttgccggttt tgactgcctg ccagtccaat
    21541 cgtacgcagg tttgccacgg cagaaaatct gatgccgacc ggagtcaaag ctcggacggc
    21601 tgtttcagcg aaccgaggta aatcgccgaa agcgtcagca ccacccctgc ccactgcacg
    21661 ccttcaatcg ttttgcccaa tccgaaataa tcgatgaaca gggcggcgac cggttcggac
    21721 aaaagcaaca gccccgtcag cgacagggaa agcagcggaa tcgcataggc aaccatcgcc
    21781 cacgcaaaac actgcatcac cacgccccgt acacaagcac caaacccgaa tctttccaag
    21841 tggtcggata aagtgcgccg ccgtccatca gcaatgccgg aacaatcggc gatgccgcgc
    21901 cgcccaaact caaaatcatc attgaaggga aaagcgccac ctgttcgact tcatgagtct
    21961 tgcggacgaa caccatagat agggcaagca tcagcccgga caccaaaccg ctggcgaatc
    22021 cccaaaccgc attaccgttg tagccgaatt ccgcaccggc aatcatcgcc acgcccacaa
    22081 ccgccgacat caagcttgcc gcctgcagcc tgcccaaacg ctcgccgaaa aagaaaacac
    22141 cgattgccga caagaaaaag atttgcaggc tgttgagcag ggtggaaata cccggcccga
    22201 ccgcgtgtat gctttcgtgc caaagtgcca aatcgaaagc gagaaacacg cccgccgtca
    22261 gggcatatcg gacggttttt ctgttttttg ggaatttttg tccgaaaaac cgtgccaaaa
    22321 accaaaatac gaataccgaa atcagcaacc gccaaaatgc gattgcatac gggccgacgg
    22381 ggacggatct gacaatcagg ctgcccaatc cgaacaccgc gcaacccagc accagcaatg
    22441 gtgcggcata tttttttccg ttaccgacca caatatcccc tcccttcccc atatggctgc
    22501 cgcgccgccg gcaaaagtgc cgccattccg aaaatatgcc gtctgaagcc caaagccccg
    22561 gctgcgcccg gacaaacggc tgactttacg ggcaggccgg ctattttccg ttggggcaaa
    22621 atccgtcctg ccggataaaa cccgaataaa aaatatttcc ctcaagcaat gcggcggcgc
    22681 caacacctga ttaaaacaca accggcgttt tcggtatata tgcaaaaacg cattttccgg
    22741 cacggcgtta caccgatacc gaaaaatgcg tctgaatgtt tcgacaatcc gattaaagca
    22801 ttatgccaaa tctgcaaaaa gtggcgtaga gagatagcgt tcgccataag aaggcagcag
    22861 cacgactatc agcttgcctt cgttttcagg ctgtttggca agctgcaacg cgctccaaac
    22921 cgccgcaccg gaagaaatgc ccgccaaaat gccttctttt tccgccattg cacgggcggt
    22981 ttcaaaagcc gcttcgttcg gcactttggt aatgctgtcg tagatttcgg tattcaaaac
    23041 ggtcggaata aaacctgcgc cgataccttg aatcgggtgc gggccttttt cgccgccgct
    23101 caatacgggg gaagcctcag gctcgacggc aaccacttta acttcgggtt tgtatttttt
    23161 caatacttcg cccacgccgg taatcgtacc gcccgtgccg acgccggcaa cgaagacatc
    23221 gactttaccg tcggtatcgt tccaaatttc ctcggcggtt gttttgcggt ggacttcggg
    23281 atttgcctca ttgtcgaact ggcgcggcat aaaataagtg tctggatgag cgtccaccaa
    23341 ggattgcgct ttggcaatcg cgcccgccat accttcggcg gcgggggtta gaatcagttc
    23401 cgcgccaaac gtgcgcaaca gcattttgcg ctctttgctc atgctttccg gcatggtaat
    23461 cgccagtttg tagccgcgtg cggcacatac cattgccaaa ccgatacccg tattgccgct
    23521 ggttgcttcg acaatgacgg tgtttttgtt gatttttccc gctttttcgg cggcctcgat
    23581 cattgcttcg gcaatgcggt ctttgacgct gctgcccgga ttaaaaaatt ccagtttcac
    23641 ggcaacctct gccttcaaac ctttggtcaa acggttcagt ttgaccaaag gcgtgttgcc
    23701 gatcaattcg gtgatgctgt ttgcaatttt catcttctgc tcctgaataa taaggtttcg
    23761 acagtaaggc gcaatcccca tcggtatcct gtccgattgc aggggatttg cgccgaacca
    23821 taaacaaatt tcggatatgg ctttatccta aacctatgcc gcctaaaaaa ccaataccca
    23881 aaatttattt ctttataact ttttgttccg atacagacgg catcacaatc atctgccgat
    23941 ttcggcaagc agcatcgccg ttaccgcaac ctgcatcaac atcgccgcca acacgccgag
    24001 tcgcagctgc gaaccgccga acgttgacgg cggctgccgg tctataccgc agcggtggac
    24061 ataggcaacc gccatcaata caaactgcaa cacgaaccaa taaaccggct tcagcgcata
    24121 aagtttgagc aatgccgcaa acacaaacac ttgaaccgtt cccgaaaagc aataatgcac
    24181 caacgcacat attccgacaa aagcaagcgt catcgataca ttactgacgg caaaaagccc
    24241 gagcagcgga tgttcggggt ctgcctgcca tccgtttcca tctgttttcc ggttccaata
    24301 cccgatgaaa aaaaatatgc tgcccaaagt caggtaaaaa tgggggatga acaaaggcgc
    24361 ggcgcgggtc attccccaca tccccggcat cagctttacc cctaaaaccg agatgccgag
    24421 ccacaacgca atactcgccc aaaaccattg caacattcct gcgcggaata tcagaaacag
    24481 aaaaaccgcc gcatacagta aggcagacaa aaaatccaaa tgcagcaaaa tcatgaatac
    24541 gccaactcgc cttcaaacac ggtttccgca gggccggtca tcatcacatc gccgccgcag
    24601 gcccattcga tatataaagt cccgcccggc aaaaccacct ctaccgtttt cccttcatcc
    24661 aacagcccca gacggatacc cgccaccaca gccgcacacg cgcccgtacc gcacgcttgg
    24721 gtttcgccca cgccgcgctc gaacacgcgc aaacggattg ccgtccgtcc gacaacctgc
    24781 ataaagccga cgttgacgcg ttcgggaaac tgcctgtgcg gttcgatacg cgaaccggtt
    24841 tcgcgcaccg gcgcgcattc cacgtcatcg actacaatca cagcatgagg attgcccata
    24901 ttgacgcagc tgacaggctg aatgccggat tcgagatgca ccccgtaaat acaggcatca
    24961 tccccctcgc ccgattcggg gacaaacggt atttcagacg gcataaactt cggtttgccc
    25021 atattgaccg taaccatgcc gttatcggac aatttcggaa aaataatgcc cttggccgtt
    25081 tcaacacaaa tttctttctt atcggtcaaa cccttgtctg caacaaaacg ggcaaaacaa
    25141 cgcgctccgt tgccgcattg cccgacctcg ctaccgtcgg cattgaaaat acggtaacgg
    25201 aaatccacgc cttcggtttc cgaacacccg accaccaaaa gctggtcgaa gcccacgccc
    25261 cggaagcggt ccgcccatgc cgcaatcggc acgtcctcgg gggtaaaatc ctgactgacc
    25321 gcgtcaatca ccataaaatc gttgcccaaa ccgtgcattt tggtaaattt caaagttttc
    25381 atattgttcc gaaatcccgt attgtctgtt gcttccgaat ccgaaatccc cgagcccgaa
    25441 gggaaatgct tccatctccc gccgcctgag gattaaaatg agccattata ctgtttgtta
    25501 ccccttacaa gaaacacaat gtcccgcctg ttcctgctgc ttttcccttg ggcaaccctt
    25561 gccgcctgca cccgaaacat accggcaagc cccgccgttt caaattgcgc gcatcttcac
    25621 aaccgcaccg tcctgtattt tgacgcacac ggcaaacccg ccgacagcac agcaaagggc
    25681 ggattttaca ggaccgtcga aattcatgac acaaaccgca tccttattca agactttaac
    25741 agcacgggga ataccctcgt caaaccctat tggtcggact gccggaaatt gtccgttttc
    25801 cccgacaaac atctgaccat gagccatcca tgaaccacga catcactttc ctcaccctgt
    25861 tcctgctcgg tttcttcggc ggaactcact gcatcggtat gtgcggcgga ttaagcagcg
    25921 cgtttgcgct ccaactcccc ccgcatatca accgcttttg gctgattctg ctgcttaaca
    25981 caggacggat aagcagctat acggcaatcg gcctgatgct cggattaatc ggacaactcg
    26041 gcatttcact cgaccaaacc cgcgtcctgc aaaatatttt atacacagcc tccaacctcc
    26101 tgctgctctt tttaggctta tacttgagcg gtatttcttc cttggcggca aaaatcgaga
    26161 aaatcggcaa accgatatgg cgcaacctga acccgatact caaccggctg ctgcccataa
    26221 aatccatacc cgcctgcctt gctgtcggaa tattatgggg ctggctgccg tgcggactgg
    26281 tttacagcgc atcactttac gcgctgggaa gcggtagtgc gacaaccggc ggactgtata
    26341 tgcttgcctt tgcactgggt acgctgccca atcttttggc aatcggcatt ttttccctgc
    26401 aactgaaaaa aatcatgcaa aaccgatata tccgcctgtg tacaggatta tccgtatcat
    26461 tatgggcatt atggaagctt gccgtcctgt ggctgtaagc cccattcatt cccgataccc
    26521 gtaaaaaata ccgtctgaaa cctgttcaga cggtattttc accctaagcg ttactcggcg
    26581 gcattttctt cacggacagg cgcatccagc agggctttga tggacagacg gacacggcct
    26641 ctgtcatcca cttccaatgc tttcacgttc accacctgac cgacttgcag gtaatcgccg
    26701 acattgcgta cgcgctcgtg tgcgatttgg ctgatgtgta ccaaaccgtc tttgcccggc
    26761 atcacgctga caatcgcgcc gacgttgttg tcgaggattt taaccacagt accttcgtac
    26821 actttgccca cttccacttc ggcagtgatt tcttcgatgc gttttttcgc tgcatcgccc
    26881 gcttcttgag tagtcgcagc gatagtgatg gtaccgtctt cagcgatatt gatttcagta
    26941 ccggtttcag cggtaatcga acgaatggtt tcgccgcctt taccaatcac atcacggatt
    27001 ttgtcttggc tgattttcat ggtgaacaaa cgcggcgcgt gtgcggacag ctcttgcgga
    27061 cccgcaacgg cagctttcat ctgatccaag atgtgcaaac gcgcttcttt ggcctgtgcc
    27121 aaagcaattt gcataatctc tttggtaatg ccttggattt tgatgtccat ttgcagcgcg
    27181 gtaacgcctt cggtcgtacc ggccacttta aagtccatat cgcccaagtg gtcttcgtcg
    27241 cccaaaatgt cggtcaggac ggcaaactta ttgccttcca aaatcagacc catcgcgata
    27301 ccggcaacgt gtgctttcaa aggcacgccg gcagacagca ggctcaaaca gccgccgcag
    27361 acagaagcca tagaggaaga gccgttggat tcggtaattt cggagaccac gcgcatggtg
    27421 tagctgaaat cttcaggttt cggcaatacg gccaacaacg cacgtttagc taaacggccg
    27481 tgaccgattt cacggcgttt cggcgcgccc atgcggccca cttcgccggt agagtacggc
    27541 ggaaagttgt agtgcagcat aaagcggtcg gtatattcgc cggacaacgc gtcgatgatt
    27601 tgttcgtcgc gcgaagtacc caaagtcgct acggcaaggg cttgggtttc gccacgggta
    27661 aacagtgcgg aaccgtgcgt gcgcggcaat acgccggttt ggatgttcag cggacggacg
    27721 gtgcgggtgt cgcggccgtc gatgcgcggt tgtccgtcca aaatttggct gcggacgaca
    27781 tcggcttcca agcgtttgaa aatacctttg atttcgttgg ctgccagcgt atcggtttct
    27841 tcggtaatca aggcttcttt taccgcattc caagcctcgt ccaatttggc ggaacgcgct
    27901 tgtttttgac ggattttgaa cgcttcttta atggtttcgc cggcaatccc gcgtactttg
    27961 gcaaccagtt cttcattggt ttcaggtgct ttccaatccc aaacttccgg attcacttcg
    28021 tcggcaaatt cattgattgc attgatggca acctgcattt gatcgtggcc gtaaaccacc
    28081 gcgcccaaca tcacgtcttc gggcaggatt ttggcttcgg attccaccat caacacggct
    28141 ttggaagtac cggcgaccac caagtccaat tgtgatttcg ccaactcggc tttagtcggg
    28201 ttcaaaacgt acacgccgtt tatataaccc acgcgcgccg cgccaatcgg gccggcgaac
    28261 ggtacgccgc tcaacaccag cgcggcggac gcgcccaaca ttgcaggaat gtcggaatcg
    28321 atttcgggat cgacggacac aaccatcgct acgatttgga tgtcgtggta gaaaccttcg
    28381 gggaacagcg gacgaatcgg gcggtcgatc agacggctgg tcaggatttc tttttcgctt
    28441 tgtttgcctt cgcgtttgaa gaagccgccg gggattttgc ctgcggcata agtgcgttcc
    28501 aaataatcga cggccaacgg gaagaagtcc tgaccttctt tgacctcttt gttggtggta
    28561 acggcaacaa aaacaacagt gtcgcccatc gatactttaa cggcagcggc agcttggcgg
    28621 gcaatttcgc cggtttccaa agtaacggtt tgattaccgt attggaaggt cttaacgtat
    28681 ttattgaaca tcattgttcc tttcaaaata ccgcactgct aaaacactaa taatgcacac
    28741 taaaatccga atgtgcatag ttagggtttc agaccgtgcg gcaggttgta aacaagcttt
    28801 cagacggcat ttcggacgct gaaagcaatt gcggattata aaggcaacca tccttaaaat
    28861 ccagtattga tacaataaaa aggccgtctg aaacatatcc ttttcagacg gccttcagtc
    28921 ttcaaaatca atcttgtgtc agtaaagtca atgcttcctg atatttctcg accgttttcc
    28981 gaattacatc ggcaggcact ttaggcgcag gggctttttt gttccaaccg ctttgttcca
    29041 gccagtcgcg gacgaattgt ttgtcaaaag acggcggatt gatgccgact tcatattgat
    29101 ccgccggcca gaagcggctc gaatcgggag tcaatacctc atccatcagc gtcagcgtac
    29161 cttcttcatc caaaccgaat tcaaatttgg tatcgcaaat gatgatgccg cgcgatttgg
    29221 catattccgc cgcttcggtg taaagccgaa ccgctttgtc gcgcacttct tccgccaatt
    29281 ctttaccgat aatgcgtccg cattcttcaa agctgatgtt ttcatcgtga tcgccgactg
    29341 cggctttagt tgagggcgta aaaatcactt cgggtagttg ttgcgcttcc cgcatacctt
    29401 cgggcagccg aataccgcaa accgagccgg ttttttgata atctttccaa ccgctgcctg
    29461 ccagataacc gcgcacaatg gcctccactt tcaccggagt gagtttttta gctacaacgg
    29521 cgcgtttttc caaagctttg gcttcgtttt caggcaaaac atcgtacacg gtatcgccgg
    29581 tgaaatggtt gggcataata tgcgccagtt ttttaaacca aaaattggaa atctgcgtca
    29641 ggatttcgcc cttacccgga atcggggcgt ccaaaatcac atcaaacgcg gacaggcggt
    29701 cggaagcgac catcagcata cgtttatcgt cgatttcata taaatcgcgc acttttccgg
    29761 aataaatctt taccaaacca atctcactca tttcgcaccc ccctgaaaat atcttgaaaa
    29821 taccgacccg acaggtttga atcgcaaacc gatattctag ccgaagtcgg cgcaaaacaa
    29881 tacccatggc acaaaaagcc aacccgtcaa ccgtcggcaa agttttggca ctataatacc
    29941 gacagcaagt cctacaatac actttcacca aaggaaatac ctcatgagaa tcctattgac
    30001 aggctcgaaa agccaactgg cacactgcct gcgcgaccgt cttcctgaag actgggaaac
    30061 cattgcgacg gattccgcat ccctagacat taccgatgcc gatgccgtct gcaacatggt
    30121 caaaagtttc caacccgacg ctattgtcaa cacggctgcc tatactgccg tcgacaaagc
    30181 ggaaggcgat gcggcagcgg catttgccgt caatgcttcc gccgtttaca accttgcctt
    30241 ggcagcacat cgcgcccatg cccgattcat ccacatctca accgactatg tctttgacgg
    30301 aaaagggaaa ataccctatc aggaaagcga ctttaccaac ccttccaatg tatacggaca
    30361 atccaaaacc gccggcgaac tgctcgcact gtctgccaat cccgacagcc ttatcctgcg
    30421 gacttcttgg ctgtttagcg aatacgggga caactttgtc cgcacgatgc tgaaccttgc
    30481 gcgggaacgt tccccgctgt ccgccgtcca caaccaaatc ggctgcccga cctatgccgg
    30541 cgacttgtcc gccgccatca tccgcctgtt gcagcagtcc aatcccgttc gcggcattta
    30601 ccactacgcc ggcggcaaat ccgtatcctg gtacgaattt gcccggcaca tctttcagac
    30661 ggcattgcaa caagatgcct cttttcccgt tcccgaactg aaagccgttt cggatgaagg
    30721 ctctgcggct gccgccccca ggcccgcata cagcattttg gactgccgca aaatcgaaaa
    30781 cgacttcggc atcaaaccgt cagactggca aaaagccctt gcacaggtcg tttccaagct
    30841 gctctgatgc cgcccgcccc tccgtttcct ccgccaagca ccgccttggc ggttttctta
    30901 taaaatctac tgattacaca aacacatatc atctttacac aatcatgctt ccatcaacag
    30961 caaaacatga tatgattgcc aacaatgaca tctcacaata aattttctaa tttttattga
    31021 aaaaatcaat aaattaagaa ttctcccaac cgacaaataa aataaagaaa gggtcaatat
    31081 gcaacaccgt agaagattgg caatttacca ggcatccaaa cgtgcttcct ttaccggcag
    31141 gtcatcagcc ccacaaaaac gtaaagaacg ttgatttgaa aaaaatgccg tctgaagtcc
    31201 tgcttcagac ggcatttttt accgttcgag aaactgtttc aacctgtcct catccaaaaa
    31261 ccagtggcaa atttcctcat ctcccgccaa caaaacggga atcagctcat tgtatttttc
    31321 ttccaaaaca ggattttcat ccacatcgac cacttccagc ccgaacccgt attcatcctg
    31381 aaaaggtttg agtgcatcgc gcattttgtg gcacaagctg caatattcac gaaacatcaa
    31441 ggtcaatttc atccgcgttt ccttatctgt caatttgcac acgccaaagc cttagacgca
    31501 gcagaatcat ggtctatttg ggaaaaaaca atgtcttcga ggaagatgat actcaagtcc
    31561 tgccaaaaac agcaaaaatg ccgtctgaac agttcagacg gcatttccaa aaccgtttta
    31621 cgcttgaacg ttgatacccg ccgtaaccgt cggttgcgca ttgtgcgccg tcagaaacga
    31681 agcggcaagc tccccgcagc agccgcagca gctcgccacg cccaattgcg actgcaaatc
    31741 gcccattgtg gtcgcgccgg cagcgatggt ttccttgatt tgatggtcgg taacggcatt
    31801 gcagatgcag acaaacattt tgtgctccgt gtgttctcaa actatcgtac cgatagcggc
    31861 ttttattatc gtatgcaaat ataaataaaa acggttcgca ttgcaaggtc ggcatacacg
    31921 gtttgtcttg gtaatttttt atcaagtttg cattttcgat tgatttctta ttaaaccgaa
    31981 gcagaagcct cagtttcggg aatggtgttc cttaaccacg ctatgcagcc gcacattggc
    32041 aacatgggta taaatctgag tcgtgttcaa atcggcgtgt cccaacatat cctgaactac
    32101 gcgcaaatcc aatccgtgcc gcaccagatg cgtggcaaag gcgtggcgca ggctgtgcgg
    32161 gctgatgtgc ccgatgcctg ccctgccggc atattctttg acaatcatcc acgccaactg
    32221 ccgggaaatg cccgtttttt tctgactgac aaacaatgcg tcgcaactcc tgcctttcag
    32281 cagaagcggg cgtgcctccg tataatagcg ttccacccaa tacgctgact cctgtcccat
    32341 cgggaccatc ctctgcttat cgcccttgcc cagcgtggta atacaacccc tgtccaaatc
    32401 cacattgccg aaattcagcc cgaccgcctc gctgacgcgc aagccggtcg cgtacatcaa
    32461 ttcgagcagg gccttatccc gcaaaccgtg cggcgtgtcg gtatccggtg cggcaagcag
    32521 tcgggaaatc tgctgttctg taatcagtgt cgggatgttt ctgtcgattt ttggcggttt
    32581 cagcaggcgg gtgggattgt ccgccctcat cccttcccgt tccatccaca catacaggcg
    32641 tttgcacgcc gacagtgcgc gtgcctgcga actccgttgc tccccgtcaa cataaaccgc
    32701 cgccgccaaa tctgcttcgt ccgcatcctt cagcgttctg ccgcacaaag acagccggcg
    32761 ggcgatcttt tccaaatccc gccggtagct gtccaaagta ttccgactga gccgccgatc
    32821 caaccacaaa gtttccagca gcctgtcaat caaaccgttt tccataacgt tccaaaccaa
    32881 tgccgtctga atcttcttca gacggcatgg ttcacatcat cgggaaagcg tttccaacac
    32941 ttcctgtgca tgacccgcca ctttgacttt ccgccactcc cggacaattt cgccgtcctt
    33001 gttcagaacg aacgtactgc gctcgatgcc taacgactct ttcccataca gttttttcaa
    33061 tttgatgaca tcaaacaggc ggcacaccgt ttcatccttg tcgctcaaca gctcgaaccg
    33121 gaaaccctgc ttggcgcaaa aattctgatg cgcctttacg ccgtcgcggg aaataccgac
    33181 cacggtataa cccaatgcct caaactgttc caaacgcgca ttaaaatcca agccttccgt
    33241 cgtacagccc ggcgtactgt ctttcggata aaaatacacg accaaaggca gatgttctgc
    33301 cgaatgaaaa tccgcaccgc tgctcgaagg cagggtaaat tcatatttca tatccatagt
    33361 cctactcccg atattcccat tattcaaaac ggcacgcaga cgcaccgccg caattgccaa
    33421 accaaccccg attctaccgc cccaaaggac aaggattcaa ccgccggaaa catccaaacc
    33481 gacacacgac ggcatgaaaa atatccatgt caaaccacaa aatatgttcc gatttaaaaa
    33541 cagaatgtta taaaacccaa ctccaaaaac acaacaagac cgcccgccta cgggcagtct
    33601 cctgtcagac gacatacttt acagatggct gttttttcaa caaaataacg ccaatactca
    33661 aaaatacgga accaaaaatg tccatccgca ccctgaaacg cctgccctca tcgctgctgc
    33721 tcggcctctg cctttccctg ccgtcagccc acctttttgc cgacaacgac attttagggc
    33781 aatttttaga acagaacatg cttacctcct ccgatccgat agaaatattc gccgaaagca
    33841 cgatatatcc cgccgatacc caggccatta cgggcgggct gattctgtcc tcccaatccg
    33901 cactggtcgt caacaacaaa acagggcaaa tactgtatca gaaaaacgcc gacaggatta
    33961 tgcccattgc ctccatttcc aagctcatga gcgcgatggt cgttttggat gcaaacttgg
    34021 acatgaacga aaccgttacc atcacacccg acgaaatcga ccgcctcaaa gggaccggca
    34081 gccgtcttgc cataggtacg gcacttacac gtaaagaact gctgcacctt agcctgatga
    34141 gcagcgaaaa ccgcgccacc catgcattgg gcagaaccta ccccggcggc atgggcgcat
    34201 ttgtcgccgc catgaaccgc aaagcccaaa gcctgggcat gtacggcagc cgcttttacg
    34261 aaccgaccgg actcaacttc caaaacgttt ccaccgccaa agacctgagc ctcatggtca
    34321 acgccgccgc ccaatatccg caaatccgca ccaactcgac ttccaactac gcctcggtac
    34381 aaaccaaaaa cgggcagcaa aactataaaa actccaatgc cctggtcagg gaaggcatgt
    34441 ggaacatcga attgcagaaa accggctaca tccgcgaggc aggcaggtct atggttgtca
    34501 aagccaacat tcaaaaccaa cccgttacca tcgtattgct gaactcgccc acatccgcca
    34561 cacgcgtcaa cgacgcccgc aaaatcgaat cgtggatgct gcagcaacgc tcctgacata
    34621 caaatgcccg gcggaaaacc gcccaaacac aaaaaaattg gtaatttttc ctattccagg
    34681 ctacaattcg acacgcacag ggcatctgcc cgatttcaca atcataatag cggagttaaa
    34741 aaatatgaca gctaaaggac aaatgttgca agatcccttc ctgaacgcat tgcgtaaaga
    34801 gcatgttccg gtttcgattt acttagttaa cggtatcaaa ttgcaaggtc aggtcgaatc
    34861 tttcgaccaa tacgtcgttc ttctgagaaa cacttccgta acccaaatgg tttacaaaca
    34921 cgccatttcc accatcgtac cggctcgctc cgtcaacctc caacatgaaa acaaacccca
    34981 agccgcaccg gcttcgaccc ttgtccaagt ggaaaccgtc cagcagcctg ccgaataatc
    35041 cgcacgaagc atgacgtgtc atatctttca ataccttacc ggacaacggt aaggtatttt
    35101 tattttcagg cggcatttaa aaatattatt gaaaaacatc cttccattcg cccattgcct
    35161 tttgcgggag gcacttcccg aaggcggcaa tgcgctggac ggcaccgccg gcaacggaca
    35221 cgacaccctt ttcctcgcac aaaccgcagg caacaggggg aaagtgtggg cattcgacat
    35281 ccagccgcaa gccctgaaca acacccgatg ccgtctgcag gaagcaggtt acagcaatgt
    35341 acggctcatc ttggacggac atgaaaacct gaagcaatat attccggagc cgctggatgc
    35401 agccgttttc aatttcggct ggttgcccgg aggcgacaag aatctgacca ccgacaagga
    35461 aaccagcatt cccgcccttt gagcaaccct ctccctgctg aaagaagacg gtatgcttat
    35521 tgccgtcctc tatccgggac acgaaaacgg caaacaggag gcagaagccg tcggacaatg
    35581 ggcaaaaaac ctgcctcaag aacagtttgc cgttttacgt tacggcttta ccaaccggaa
    35641 gaatagccca ccctatcttt tggcatttga aaaactgcgt caaaaataac tgtttgcggt
    35701 aaaataagcc gattctaacc cttattttga tacgagccga ccatgcagta tctgattgtc
    35761 aaatacagcc atcaaatctt cgttaccatc accattttgg tattcaacat ccgttttttc
    35821 ctactttgga aaaatccaga aaagcccttg gtcggctttt ggaaagcact gccccacctc
    35881 aacgacacga tgctgctgtt tacgggattg tggctgatga agattaccca tttctccccg
    35941 ttcaacgcgc cttggctcgg cacaaaaatc ctgctcctgt tcgcctacat cgcactgggc
    36001 atggtaatga tgcgcgcccg tccgcgttcg accaagttct acaccgttta cctgctcgct
    36061 atgtgttgca tcgcctgcat cgtttacctt gccaaaacca aagtcctgcc attctgaaac
    36121 actgccatga acaacagaca ttttgccgtc atcgccttgg gcagcaacct tgacaacccc
    36181 gcacaacaaa tacgcggcgc attagacgcg ctctcgtccc atcctgacat ccggcttgaa
    36241 caggcttcct cactgtatat gaccgcaccc gtcggttacg acaatcagcc cgatttcatc
    36301 aatgccgtct gcaccgtttc caccaccttg gacggcattg ccctgcttgc cgaactcaac
    36361 cgtatcgaag ccgatttcgg acgcgaacgc agtttccgca atgcaccgcg cacattggat
    36421 ttggacatta tcgactttga cggcatctcc agcgacgacc cccgccttac cctgccgcat
    36481 ccgcgcgcgc acgaacgcag tttcgtcata cgccctttgg cagaaatcct ccctgatttt
    36541 attttgggaa aatacggaaa ggttgtcgaa ttgtcaaaac ggctgggcaa tcaaggcatc
    36601 cgtcttttac cggacaggta attccgcacg cggatgccgt ccgaacaccg gtttcagacg
    36661 gcatttttcc tttgccgcca acacgcgtgc aaaaaaaaat cgccccttga gaaaggggcg
    36721 caaaaggaac acaaaccact accaaaactt taaatctgca acactgcctg ccgcatactg
    36781 tacccgacag gatataaggc cctcactaaa tcgtttcgag aaatccaaac ttcttcatca
    36841 ccgacagaaa atctgccttt ctccggcacc agctccaaca aaaacggctg aaccgccgta
    36901 tgcagcttgt ccttacaccg ccccagcttt ctggacaacg cggacagggc gcgtttgtag
    36961 gcattatcct tgcggtcgag ctccctcgca ctaccgaccc agctcaaacg aaggttgcgg
    37021 ctttcatctt caatcaacaa aatacctgtt tcaacttccc ccacctcggg ttcgcacgaa
    37081 agcgcaatat aatatttgcc gtcaatattg acatatgccg cttcatgcac ctgacagttg
    37141 caacccgagg ttgcgagcag ggaataaaca gaatcacggt gcagcctcaa aatatcggag
    37201 gaaatctgtt tgaaaatact ttccggcatt tttccctcct ttcgtctgct gcgttgttta
    37261 ctggattgac tgcattatac agacatctcc caattttaca aatgataatt gttcctattt
    37321 tttaaaaagt ttataaatta ttgataaata tatg
//
LOCUS       DAXFZR010000011        35100 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000011 DAXFZR010000000
VERSION     DAXFZR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35100
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.011"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..214)
                     /locus_tag="ACFJAS_000439"
     CDS             complement(<1..214)
                     /locus_tag="ACFJAS_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981779.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLV"
     gene            complement(276..2900)
                     /gene="alaS"
                     /locus_tag="ACFJAS_000440"
     CDS             complement(276..2900)
                     /gene="alaS"
                     /locus_tag="ACFJAS_000440"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC3981780.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPEMLASAEGWLCQKLS"
     gene            3104..4234
                     /locus_tag="ACFJAS_000441"
     CDS             3104..4234
                     /locus_tag="ACFJAS_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3981781.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4275..5177)
                     /locus_tag="ACFJAS_000442"
     CDS             complement(4275..5177)
                     /locus_tag="ACFJAS_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC3981782.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5316..5813
                     /locus_tag="ACFJAS_000443"
     CDS             5316..5813
                     /locus_tag="ACFJAS_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFC3981783.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(5875..6780)
                     /gene="mtrA"
                     /locus_tag="ACFJAS_000444"
     CDS             complement(5875..6780)
                     /gene="mtrA"
                     /locus_tag="ACFJAS_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC3981784.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6909..7244
                     /locus_tag="ACFJAS_000445"
     CDS             6909..7244
                     /locus_tag="ACFJAS_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC3981785.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7375..7845
                     /locus_tag="ACFJAS_000446"
     CDS             7375..7845
                     /locus_tag="ACFJAS_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981786.1"
                     /translation="MKYHRLALFAAISCLLLSAVFIAPYLTAFHEQEKIFEYADLTVT
                     APNRSGRAIKLEADGRQYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNG
                     VLLEYRNSGSVYSNKDFSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7951..8514
                     /gene="pgsA"
                     /locus_tag="ACFJAS_000447"
     CDS             7951..8514
                     /gene="pgsA"
                     /locus_tag="ACFJAS_000447"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC3981787.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVATVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            8595..8670
                     /locus_tag="ACFJAS_000448"
     tRNA            8595..8670
                     /locus_tag="ACFJAS_000448"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8628..8630,aa:Gly,seq:gcc)
     gene            8696..8771
                     /locus_tag="ACFJAS_000449"
     tRNA            8696..8771
                     /locus_tag="ACFJAS_000449"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8729..8731,aa:Gly,seq:gcc)
     gene            8797..8872
                     /locus_tag="ACFJAS_000450"
     tRNA            8797..8872
                     /locus_tag="ACFJAS_000450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8830..8832,aa:Gly,seq:gcc)
     gene            8898..8971
                     /locus_tag="ACFJAS_000451"
     tRNA            8898..8971
                     /locus_tag="ACFJAS_000451"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8930..8932,aa:Cys,seq:gca)
     gene            9072..9161
                     /locus_tag="ACFJAS_000452"
     tRNA            9072..9161
                     /locus_tag="ACFJAS_000452"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9106..9108,aa:Leu,seq:taa)
     gene            9265..10317
                     /gene="sohB"
                     /locus_tag="ACFJAS_000453"
     CDS             9265..10317
                     /gene="sohB"
                     /locus_tag="ACFJAS_000453"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC3981788.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLIRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(10368..11234)
                     /locus_tag="ACFJAS_000454"
     CDS             complement(10368..11234)
                     /locus_tag="ACFJAS_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC3981789.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFIRKGDERQLYRHYGLVSRLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(11245..11676)
                     /locus_tag="ACFJAS_000455"
     CDS             complement(11245..11676)
                     /locus_tag="ACFJAS_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC3981790.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(11911..12774)
                     /locus_tag="ACFJAS_000456"
     CDS             complement(11911..12774)
                     /locus_tag="ACFJAS_000456"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC3981791.1"
                     /translation="MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGSTAELVRACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTIVEAIGGSAMDSPMFQTK
                     KSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQD
                     VSGVYLKLAEH"
     gene            complement(12850..13761)
                     /gene="hisB"
                     /locus_tag="ACFJAS_000457"
     CDS             complement(12850..13761)
                     /gene="hisB"
                     /locus_tag="ACFJAS_000457"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC3981792.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSKLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(13778..14857)
                     /gene="hisC"
                     /locus_tag="ACFJAS_000458"
     CDS             complement(13778..14857)
                     /gene="hisC"
                     /locus_tag="ACFJAS_000458"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC3981793.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(14903..16192)
                     /gene="hisD"
                     /locus_tag="ACFJAS_000459"
     CDS             complement(14903..16192)
                     /gene="hisD"
                     /locus_tag="ACFJAS_000459"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC3981794.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(16189..17076)
                     /locus_tag="ACFJAS_000460"
     CDS             complement(16189..17076)
                     /locus_tag="ACFJAS_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC3981795.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(17168..17833)
                     /gene="hisG"
                     /locus_tag="ACFJAS_000461"
     CDS             complement(17168..17833)
                     /gene="hisG"
                     /locus_tag="ACFJAS_000461"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC3981796.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(17912..18565)
                     /locus_tag="ACFJAS_000462"
     CDS             complement(17912..18565)
                     /locus_tag="ACFJAS_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC3981797.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRALL"
     gene            18841..18925
                     /locus_tag="ACFJAS_000463"
     tRNA            18841..18925
                     /locus_tag="ACFJAS_000463"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18875..18877,aa:Leu,seq:caa)
     gene            complement(18988..19095)
                     /locus_tag="ACFJAS_000464"
                     /pseudo
     CDS             complement(18988..19095)
                     /locus_tag="ACFJAS_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            19642..21369
                     /gene="ilvB"
                     /locus_tag="ACFJAS_000465"
     CDS             19642..21369
                     /gene="ilvB"
                     /locus_tag="ACFJAS_000465"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC3981798.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDNKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
     gene            21380..21871
                     /gene="ilvN"
                     /locus_tag="ACFJAS_000466"
     CDS             21380..21871
                     /gene="ilvN"
                     /locus_tag="ACFJAS_000466"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC3981799.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            21938..22231
                     /locus_tag="ACFJAS_000467"
     CDS             21938..22231
                     /locus_tag="ACFJAS_000467"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC3981800.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            22311..23324
                     /gene="ilvC"
                     /locus_tag="ACFJAS_000468"
     CDS             22311..23324
                     /gene="ilvC"
                     /locus_tag="ACFJAS_000468"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC3981801.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(23443..24438)
                     /locus_tag="ACFJAS_000469"
     CDS             complement(23443..24438)
                     /locus_tag="ACFJAS_000469"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC3981802.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(24580..27165)
                     /gene="acnB"
                     /locus_tag="ACFJAS_000470"
     CDS             complement(24580..27165)
                     /gene="acnB"
                     /locus_tag="ACFJAS_000470"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC3981803.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            complement(27480..28550)
                     /gene="lptG"
                     /locus_tag="ACFJAS_000471"
     CDS             complement(27480..28550)
                     /gene="lptG"
                     /locus_tag="ACFJAS_000471"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC3981804.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(28547..29662)
                     /gene="lptF"
                     /locus_tag="ACFJAS_000472"
     CDS             complement(28547..29662)
                     /gene="lptF"
                     /locus_tag="ACFJAS_000472"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC3981805.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            29651..29833
                     /locus_tag="ACFJAS_000473"
     CDS             29651..29833
                     /locus_tag="ACFJAS_000473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981806.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            29836..31242
                     /locus_tag="ACFJAS_000474"
     CDS             29836..31242
                     /locus_tag="ACFJAS_000474"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC3981807.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            31305..31745
                     /locus_tag="ACFJAS_000475"
     CDS             31305..31745
                     /locus_tag="ACFJAS_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC3981808.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            32005..32823
                     /locus_tag="ACFJAS_000476"
     CDS             32005..32823
                     /locus_tag="ACFJAS_000476"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3981809.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGGTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGK
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            32930..33556
                     /gene="purN"
                     /locus_tag="ACFJAS_000477"
     CDS             32930..33556
                     /gene="purN"
                     /locus_tag="ACFJAS_000477"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC3981810.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADVAAGRLIIEGNRVRNSENADAARFLTA"
     gene            33572..34243
                     /locus_tag="ACFJAS_000478"
     CDS             33572..34243
                     /locus_tag="ACFJAS_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC3981811.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            34361..34783
                     /locus_tag="ACFJAS_000479"
     CDS             34361..34783
                     /locus_tag="ACFJAS_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC3981812.1"
                     /translation="MQVTSKWIDGMCFVGTSEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
BASE COUNT         8142 a         8723 c         9667 g         8568 t
ORIGIN      
        1 ataccaaact tgtatcaagg atgggcaatt ctatagttaa acctatttct tttgcgagaa
       61 tatttaattg tttgtcaatt aaccatcgac ggacaatagg agttgcaatg ctacgtacat
      121 atcttggagg aagagcatct acacgaatta gtgtttcttt taaagttgat atatcctctt
      181 ctaaaagttt ttgtaactct gttttagtat tcattctaac aatccaaaat ataagccatt
      241 tgaacaagac tcaaatggct tattaagatt taaatttaac ttaatttctg acaaagccaa
      301 ccttccgcgc ttgccagcat ttcgggcaat ttgtcggcat ccgtaccgcc ggcttgcgcc
      361 aagtcgggac ggccgccgcc tttgccgccg acttgttcgg ctgcaaattt aaccagatct
      421 cctgctttga cttttccggt cagggctttg gatacgccgg cgcacaggga gactttaccc
      481 tcgtttactg ccgctaaaag aatcacggcg ttgtcggatt ttccggtcag gtcggttacg
      541 gtttcgcgca gggcggctgc gtcggcttcg atttgtgcgg caacgagttt ggccgcgccc
      601 aggtcttttg cgtcgtccaa gagtttggcg cctgcgtgga cggcgagttc ggctttggcg
      661 cgtgccaatt ctttttccaa tgctttggcg tgtgccgcgc ctgcttggat tttcgccagt
      721 acgtcttttt cggtttgggc tttggtttcg gcaatgatgt cttttaccaa acgctcttgc
      781 tcttgcgccc atttgagtgc gttcaggccg gtgatggctt cgatacggcg tacgcctgcg
      841 gcaataccgc cttcgctgat gattttgaag aggccgatgt cgccggtgcg tgaaacgtgt
      901 gtgccgccgc acaattcggt agagaaaccg cccatttgca gtacgcgtac ttcgtcgccg
      961 tatttttcgc cgaagagcat catggcgtcg gttttttgcg cgtcttccat gctcataatg
     1021 gctgcgttga cggcaacgtt ggctaaaacg gcttcgttga cgcggcgttc gacttcagcg
     1081 atttcttcgg cggttaccgc ttggggatgg gaaatgtcga aacgggtgga ttcggcggta
     1141 accaaagagc ctttttgttc gacgtgtccg cccaatacgt cgcgcagggc tttgtgcatc
     1201 aggtgggtcg cgctgtggtt gcgcatattg gcattgcgga tttcgtcatc cactttggcg
     1261 gtaacgctgt cgccgacttt cagacggcct gaagtttgta cgccgaattg tccgaatacg
     1321 gccgctttga ttttttgggt atcgcgtact tcaaagcggt tttcgcctgc gaagatatag
     1381 ccgacgtcgc cgacttggcc gccggattct gcatagaacg gggtaaagtc gataacgacg
     1441 gcaccgctgt cgccttcgtt caattcgacg acttgcccgc cgtctttgta gagggcgagg
     1501 actttggatt cggtttggcg ttcgctataa cctttaaact cggtgtcttg accgtcgtaa
     1561 ggcagttggg cgttggcttt gaagctttgg gcggcgcgtg cgcgtgcgcg ttgggcttcc
     1621 atttcgcgct cgaagcctgc ttcgtccggt tcgatattgc gttcgcggca gatgtcggca
     1681 gtcaggtcgt atgggaagcc gtaggtatcg tagagtttga agataatttc gccgccgagt
     1741 gttttgccgc ctttggccag cgcgttttcc aacaaagcca taccggtttc cagagtttgg
     1801 gcaaaacggc tttcttcgtt tttcaaagct tcttcgattt gcgcctgttt ttctttcaat
     1861 tcaggatacg cgccgcccat ctcttgaacc aaatcggcaa cgagtttgtg gaagaacggt
     1921 ttgctttgac ccagtttgta accgtggcgc acggcgcggc ggatgatgcg gcgcaatacg
     1981 tagccgcgtc cttcgttgga aggcaatacg ccgtctgcaa tcaggaatga gcaggagcgg
     2041 atgtggtcgg cgataacttt caggctgggt tcttccatac tgaacggcgc gccggtttcg
     2101 cgggcaacgg ctttgagcag gtcttggaac aggtcgattt cgtagttgct gtggacgtgc
     2161 tgcatcacgg cggccatacg ttccaagccc ataccggtat cgacggacgg cttgggcagt
     2221 ggattcatat tgccttgttc gtcgcggttg aactgcatga atacgcagtt ccaaatttca
     2281 atccagcggt cgccgtcttc ttcggggctg ccgggaatgc cgccccagat ttctttgccg
     2341 tggtcgtaga aaatttcgga gcaggggccg caagggccgg tgtcgcccat ttgccagaag
     2401 ttgtcggacg cgtatttcgc gcctttgttg tcgccgatgc ggacgatgcg ttcagacggc
     2461 ataccgattt cgttcaacca gatgttgtag gcttcgtcgt cttctgcgta aacggtcgcc
     2521 aagagtttgt ctttggggat gttgagccat tcgggggaag tgagaaactc ccaagcgaag
     2581 tggatcgcgt cgcgtttgaa gtaatcgccg aaggagaagt tgcccatcat ttcaaagaag
     2641 gtgtggtggc gggcggtgta gccgacgttt tccaagtcgt tgtgcttgcc gcctgcgcgt
     2701 acacattttt gcgcggtggt ggcgcggctg tacgggcgtt tgtcgaaacc taaaaatacg
     2761 tctttaaact ggttcatgcc tgcgttggta aacagcaggg tcgggtcgtc gtgcggcacg
     2821 aggctggaag agcggacgac ggtgtggcct ttggtttcaa aaaattttag gaatttttgg
     2881 cgcagttcgg aggttttcat aattttttca atgtctctca aatgtcttgt catggtaaaa
     2941 gcagggaaaa cgaacggcgg tatattaccg caaatccctg tttctagcta tggaagcggc
     3001 ggctttcaga cggcattgcg ggattttgaa tgccgtctga agccctgtta ccaatatcgg
     3061 ctataatggc cgctttctcc aacccgatat gcaaggaatg ataatggtca aacatctgcc
     3121 actcgccgtc ctgactgctt tgctgcttgc agcgtgcggc ggttcggaca aaccgcctgc
     3181 cgaaaaaccg gcaccggcgg aaaaccaaaa cgtattgaaa atttataact ggtcggaata
     3241 cgtcgatccg gaaaccgttg ccgattttga aaagaaaaac ggcatcaagg ttacttatga
     3301 tgtgtacgac agtgatgaaa cgctggaaag caaggtgctg accggaaaat ccggttacga
     3361 cattgtcgcg ccgtccaatg cgtttgtggg caggcagatt aaggcaggtg cgtatcagaa
     3421 aatcgataag tcgatgattc ccaattataa acatctcaac cctgaaatga tgaggctgat
     3481 ggacggggtc gatcccgacc acgaatacgc cgtgccgttt tattggggga caaatacctt
     3541 cgccatcaat accgaacgcg tgaaaaaggc tttgggtacg gacaagctgc cggacaacca
     3601 gtgggatttg gtgttcaacc ccgaatacac gttcaaactc aaacaatgcg gcatcagcta
     3661 tttggacagc gcggcggaaa tttatcccat ggtgttgaac tatttgggta aaaacccgaa
     3721 cagcagcaat acggaagaca tcagggaggc aaccgccctg ctcaagaaaa accgccccaa
     3781 tatcaaacgc tttacttcgt ccggctttat cgatgatttg gcgcgcggcg atacctgcgt
     3841 aacaatcggt ttcggcggag atttgaacat cgccaaacgc cgtgccgaag aagcgggcgg
     3901 caaggaaaaa atccgcgtga tgatgccgaa agagggcgtg gggatttggg tggattcttt
     3961 cgtgattccg aaagatgcga aaaacgtcgc caacgcgcac aaatacatca acgacttcct
     4021 cgatccggaa gtgtcggcga aaaacggcaa tttcgttacc tacgcgcctt cgagcaagcc
     4081 ggcgcgcgat ttgatggagg acgaatttaa aaacgacaat acgattttcc cgagcgggga
     4141 agatttgaaa aacagcttta tcatggtgcc tatccggccg gcggcattga agtttatggt
     4201 gcgccagtgg caggatgtga aggcggggaa ataaagcccg atatgccgtc tgaaggatgt
     4261 tcggacggca ttttttatct ttggcggaag agggcttgca gccgctgttt gaaggcaatg
     4321 gggcggatgc tgctcaaaat gccgctgagg ataatgatgc acataccgag tatttcctgc
     4381 cagaaaagct cttcgcccag aaaaaatgcg gcagacaggg cggaaaagac gacggtcata
     4441 taggaaagcg aggcaaccgt gaatttgtcg ccgactttgt aggcgcgcgt catcgacagt
     4501 tgggcaatca gcgcggacac gccgatgccc gacagataaa ccgccgatgg aaaggacagg
     4561 gtgtgccagc cggtcagcgt cgcccaaacc gacgacatcg ccacgccggt tgcggaaagg
     4621 taaaacacga cgcgccagcc gggttcgccc gccaaagaca gttcgcgcac tttcaaatac
     4681 gcccagccgg acatcgcgcc gcccgccagc ccggcgagtg ccgccggttc ctgaccgctg
     4741 cggaacgagg gattaagcag caataccacg ccggcaaaac caaggagcag caccgcctgc
     4801 gtgtaaacgg aaatccgttc tttcaaaatc aggaaggaaa ataccgccaa aaaaatcgac
     4861 gaggtgtaac tcagggtaac gccggttgtc aaaggcagat gcgttaccgc gtaaaacagc
     4921 agcagcatcg cccccgtccc gaccatactg cggtttaagt ggtttttcca atggggcgtg
     4981 cggaaggtgt cgcgccgcaa tacggcggca gcaccgagcg taacggttga aaacagcatg
     5041 cgccaaaaga ccaattcgcc gctgccgagg gcaaattttg ccgatgcctc tttaatcaat
     5101 acgttcataa cggtgaagca ggccgccgcc accagcatcc agcccgatcc taaaatgtct
     5161 ttttttgcgg tatccataaa cggtcgttgc gataaggacg gtcggattgt aaaccttgcg
     5221 gcaaggcttg tggaatgtgt ttttgcctgc ttctgatgcc gaaattttat ttttcttgcc
     5281 gaacaatttg ttttctcaag gcaaacttga ttataatggc gggtatgaaa aaatacctta
     5341 tccctctttc cattgcggca gtcctttccg gctgccagtc tatttatgtg cccacattga
     5401 cggaaatccc cgtgaatcct atcaataccg tcaaaacgga agcacctgca aaaggttttc
     5461 gcctcgcccc ttcgcattgg gcggatgttg ccaaaatcag cgatgaagcg acgcgcttgg
     5521 gctatcaggt gggtatcggt aaaatgacca aggttcaggc ggcgcaatat ctgaacaact
     5581 tcagaaaacg cctggtcgga cgcaatgccg tcgatgacag tatgtatgaa atctacctgc
     5641 gttcggcggt agacagccag cgcggcgaaa tcaatacgga acagtccaag ctgtatatcg
     5701 agaatgcctt gcgcggctgg cagcagcgtt ggaaaaatat ggatgccaaa cccgataatc
     5761 ccgcatttac caactttttg atggaagtga tgaagatgca gcccttgaaa tgacgcggta
     5821 cgcaaatgcc gtctgaaagc tttttcagac ggcatttgcg tttgaagccc cgatttattt
     5881 ttgcccgcct tctttccggt attgccccgg cgaaacgtga tattgccgtt tgaacgcctt
     5941 gccgaaatgc gtttccgact gaaagcccac cgacagcgcg acctccaaaa ccgaatccgg
     6001 ggttttcttc agcagcaatg cgcctttttg caggcggata tggttcacaa aggcgtgcgg
     6061 gctgagtccg acttggcttt tgaagcggcg catcagttgc gcgcgcgaca tattggcggc
     6121 ggcaaccatt ttgtcaatat tccattcgtc ttccggtttg tctatcacct tttggatcaa
     6181 atgtcccaaa cgtttgtcct gccaaccttt caatacgccc gagagttcga catccttatc
     6241 ctgttcgaga taggcgcgca ggataagcac cagcaggacg gacggtaatg cgttgaccac
     6301 ggaaaccgtc cccgtcaaag gtttttcgct ttccagttgc agcattgaaa ccacatactg
     6361 caaacttgga tgggcaatgt tcagaaaaac ggtttccggc agcccgttca tcaaatcggc
     6421 gtgggtgtcg tagcggaaac gggcgcaaaa caggctcata tccagcccgt tgccgcactg
     6481 tttgaccata aatgtgccgt tttgccgtat gtccggttgt aaactttctc cgtattttcc
     6541 gtcgtggctc aacacatgac ccaagccgcg cgggaaaaat acaatatcgc ccgtgccgac
     6601 cggacgcggg gaagtttcgc cgtcgatgca gagataaccg ctgcccgccg taacaatgtg
     6661 taccagccct tcgcattgca aggtttcatg ccgtaccgac cattgtccgc ccaaaaggca
     6721 ctgcacatcc gcactgcccg tcaattgggc gagatcgacc agtttgtcca gaatgtccat
     6781 aaatcttttt gaaccataaa atgagatgat taaacgagaa acacaactga ataattgcaa
     6841 taatacgcac atcaaaaaca gatacgcaag cgcgtatcag ggttaaacgc aagagaaagg
     6901 aaaagaaaat gtttaaagat tggaaagaac ataccgcatt ggttaaaaaa tcattcggcg
     6961 agctgggtaa ggcgcatcct aaaatgctgc aagcctacgg cgcattggaa caggccgccg
     7021 ccgccgaagc actcgatgcc aaaacgcgcg aactgattgc catcgccgtt gccatcacca
     7081 cccgttgcga gagctgcatc agcgttcacg ccgccgccgc cgccaaagcc ggtgcgaccg
     7141 acagcgagat cgcaggtgca ttggcaaccg ccattgccct gaatgcgggt gctgcttaca
     7201 cttacgccct gcgtgcattg gaagcggttg aaacgcaaaa ataatccgtt tcggataaga
     7261 aatgccgtct gaaaatattt cagacggcat tttgcctgtt atgcttcccg atattttgca
     7321 gacaacatat gcagccgttt ttttcagacg gcatttgaga aacatggaac aagtatgaaa
     7381 tatcaccgct tggcattgtt tgccgccata agctgcctgt tgttgtccgc cgtttttatt
     7441 gcaccttacc tgacggcatt tcacgaacaa gaaaaaatat ttgaatatgc ggatttgacc
     7501 gttaccgccc ccaaccgcag cggacgggca atcaaattgg aggcggacgg gcggcagtac
     7561 cggctttcct gctacgggtt cgacagtttg tgcacaggcg gcaatatcgg cagagccatc
     7621 agggcgcggc aggttaagat tgttttaagc gaaaccgtcg gcaaaggttt tttaaacggc
     7681 gttctgttgg aataccgcaa cagtggcagt gtctatagca ataaagattt ttcccgcacg
     7741 gaagaccgcc ttgtcgaagt gttggcacaa cctgccgttt tcagcctgaa accgggtatt
     7801 ttgcttttat tgcccgccat tttcctgcgt ttgaaaaaaa tgtgaaaaca gattggcggg
     7861 gcgggggaat gactatgcgg caattttacg ttttgggtta tgaggataac cgttataatc
     7921 ataattttac agtccgcaca gagaaaaccg atgccttgga acatccccat cttcctcaca
     7981 tggttgaggg tcttgctcat tcccgtactg attgtccttt tctacctgcc gttctcatgg
     8041 ttttcagaag aagcagtcaa tgttgccgcc gccgtcattt ttgccgttgc cgccttgacc
     8101 gactggtttg acggattttt ggcaaggttg tggaaacaga cttccgattt cggcgcattc
     8161 ctcgaccccg tcgccgacaa gctgatggtt gccgtatcgc tgctgctgct ggtcaaactc
     8221 gaccggacct atgttttgtt cgcgatgatt atcatcggca gggaaattac catttccgca
     8281 ttgcgcgaat ggatggcgca aatgggaaaa aggagcagcg ttgccgtcgc caccgtcggc
     8341 aagttcaaaa ccgccgcgca aatgctggcg attttctttt tgctgctgaa ttttcccgat
     8401 ttttacggat ttaatcttgt tgttatcggc aacatattga tgtttatcgc atctttgctg
     8461 acagtctggt cgatgctgta ttatctgaaa atggcgtgga aagaaattgc ctgaaaaaaa
     8521 cataaaaaca gcttgacggt aaaaacataa tctataataa ttgcgtcttc ttcgatgtcg
     8581 gagagtgaaa tcgggcggga atagctcagt tggtagagcg caaccttgcc aaggttgagg
     8641 tcgcgagttc gagactcgtt tcccgctcca aaaatttcat tcttccgtaa tcattgcggg
     8701 aatagctcag ttggtagagc gcaaccttgc caaggttgag gtcgcgagtt cgaggctcgt
     8761 ttcccgctcc aaaaatttca ttcttccgta atcattgcgg gaatagctca gttggtagag
     8821 cgcaaccttg ccaaggttga ggtcgcgagt tcgagactcg tttcccgctc caagttttgt
     8881 agacaagcca gttttagggc gagatagcaa agtggttatg cagcggattg caaatccgtc
     8941 tacgccggtt cgattccgac tctcgcctcc atatatctta cggcggggtg gcagagtgtt
     9001 tatgctatga ggggtgcaac cttcatatag gccggttaaa atccgcgccc ccgcctccac
     9061 ctttcacaaa tgcccgggtg gtgaaatagg tagacacaac ggacttaaaa tccgtcggga
     9121 ctaaacatcc catgccggtt cgattccggc tccgggcacc aagctgaaaa tgacaatgcc
     9181 gctccaagcg gttatttttt tatctgtcgg atggggatgg acaatattga tatgttcatg
     9241 cctgaacaag aggaaatcca atcaatgtgg aaagaaattt tactgaatta cggtattttc
     9301 ctgctcgaac tgcttaccgt gttcggcgca attgcgctga ttgtgttggc tatcgtacag
     9361 agtaagaaac agtcggaaag cggcagtgtc gtactgacag atttttcgga aaattataaa
     9421 aaacagcggc aatcgtttga aacattcttt ttaagcgagg aagagacaaa acatcaggaa
     9481 aaaaaagaaa agaaaaagga aaaggcggaa gccaaagcag agaaaaagcg tttgaaggag
     9541 ggcggggaga aatctgccga aacgcaaaaa tcccgccttt ttgtgttgga ttttgacggc
     9601 gatttgtatg cacacgccgt agaatccttg cgtcatgaga ttacggcggt gcttttgatt
     9661 gccaagcctg aagatgaggt tctgatcaga ttggaaagtc cgggcggcgt ggttcacggt
     9721 tacggtttgg cggcttcgca gcttaggcgt ttgcgcgaac gcaatattcc gctgaccgtc
     9781 gccgtcgata aggtcgcggc aagcggcggc tatatgatgg cgtgtgtggc ggataaaatt
     9841 gtttccgctc cgtttgcgat tatcggttcg gtgggtgtgg tggcggaagt gccgaatatc
     9901 caccgcctgt tgaaaaaaca tgatattgat gtggatgtga tgacggcggg cgaatttaag
     9961 cgcacggtta cttttatggg tgaaaatacg gaaaagggca aacagaaatt ccggcaggaa
    10021 ctggaggaaa cgcatcagtt gttcaagcag tttgtcagtg aaaaccgccc cgggttggat
    10081 attgaaaaaa tagcgacggg cgagcattgg ttcggccggc aggcgttggc gttgaacttg
    10141 attgacgaga tttcgaccag tgatgatttg ttgttgaaag cgtttgaaaa caaacaggtt
    10201 atcgaagtga aatatcagga gaagcgaagc ctgatccagc gcattggttt gcaggcggaa
    10261 gcttccgttg aaaagttgtt tgccaaactt gtcaaccggc gagcggatgt gatgtagttt
    10321 gccgaacgga tggaaatgcc gtctgaagtg tgttcagacg gcatttttca agttccggct
    10381 ttgatgttcc gttccgacca ctctatccgt tgtccgatgt ctttcaaatt ggaatattgt
    10441 tcgaccaatc gcggcacatc gtccaaactc atggcaaaca tccacagata agtgccgacc
    10501 gaataaatat gccccgcgct gccgtagcct ttgagcgtca tcatcacaaa agcaaagccg
    10561 aacaaaatac ccatcgccgc gccgacgcag agatagccga aggcttcgcg gttggaaatc
    10621 agcacacgca ggcgcgaaac cagtccgtaa tggcggtaca gctgccgctc gtcgcctttt
    10681 cggataaagt ggttgtcgcg ttccaagctg ttgttcaggc ggaaatacag gttttcgctg
    10741 atggcggcaa aacgtggcaa aagccataaa aacaacgcaa gtatgcccac cgccgacacg
    10801 ccgacccaaa attccagcac cagcagcatg atgcacgcgc cgaatatgga tacgacggat
    10861 gtcgcggcaa tcggcaggtg ttcttcaaaa aagctgacaa attcacgcga cagggcaacc
    10921 cgtgcagtta ccgctgaatg cgggacttgc cgctgccgtt gttccaacac aaccggcacg
    10981 gcgatttcgg tataaatccg cgtaaacgtg cgcgtatcgg caatccgccg tgccgcaccg
    11041 accagccaca tcaaaaatac aaccaaagcg tacagcaacg cctgccacac cctccccgca
    11101 atcacggcat taatcgccca gccgccaaac accggatacc ccagcatcaa aaggttttcc
    11161 agtccgaccg gggaaaatgt gccaatcaat cgcttgcggt gggttttggc tatgtgtttc
    11221 aacattttcc acatatttgt ccctctattt tttattttcc gagattgttt tttccatcac
    11281 ttccgccagt gcatccaaat cggcaaacag ccgggttgtg cgcttgtcgc cgaatgtgga
    11341 aaatactttg tcgctgaatt cctgcgcgtt ttctgttaaa ggtgcggcat ggactttgcc
    11401 tttttcggtc agcgacagca accgttcgcg cctgtcctgt tcgccttcct gccattcaat
    11461 caacccttgt ccggcaaggg ttttgcatac gcctgaaacg gtctgtttgg gcagactcca
    11521 ctcttctccg atgtgctttt gcgtgcggct gccttcggtt gccagggtat aaagtacggt
    11581 aaagaggttg taattcaaat cctgctgccc gatccatttg tcaaagacat tgcaaatcag
    11641 gttgatacgg attccaagtt ggtcgagtcg gttcataatt ggtcttgata ttgactaaat
    11701 ttgtgcggat tatagtggat taacaaaaac cggtacggcg ttgcctcgcc ttgtcgtact
    11761 atttgtactg tctgcggctt cgccgccttg tcctgatttt tgttaatcca ctatatagtc
    11821 ataatcggga ctgatttcaa gcgtggggcg ataaaaatgc cgtctgaaac gctttctggc
    11881 ttcagacggc atattgtttg gaaagggaag tcagtgttct gccaatttca ggtaaacgcc
    11941 ggaaacgtcc tgttcgccgt agccggcttc aactgctttg cggtagctgg cagcaacggt
    12001 ttcgacggcg ggcagggtgt tgcctgcctg ttcaagctct ttgacggcga ggttaaggtc
    12061 tttggaagcg tgtttgagtg caaaggcagg ggggaactca cggtttgccc atagtgattt
    12121 ttttgtttga aacataggcg agtccattgc cgagccgccg atggcttcga cgatggtatc
    12181 gatgccgaac tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcctaa
    12241 gagcgagttc aagacgagtt tcgcgcccga gcctttgccg acatcgccga aatggaaggt
    12301 ttttttgccg acaagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc
    12361 gaacagaatc agcagtgtgc cgttggtggc gggtccgacc gatccggaaa cgggtgcttc
    12421 ggcaaactgt ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga
    12481 gatggtgctc atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat
    12541 gtcgcacacg gcggcatagt cggaaaccat caggaaaatg acggggcagg cgcggacgag
    12601 ttcggcggtg ctgccgtaaa cttttgctcc tttggcggag atgggggcag ttttgtcggg
    12661 cgagcggttg tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag
    12721 acccatttgc cctaagccta cccagccgat ttgtgtgtat gtttctgcgg acatagtgtt
    12781 tctcctttgt tggggcgctg taatgccgtc tgaaggcttc ctgcgcttca gacggtctgt
    12841 ttggggatgt tatgcggtca gcgtgccttt ggtcgagggg gtttgcccag ccattctcgg
    12901 gtcgtgttcg actgccatac gcagggcgcg cccgaaggct ttgaatacgg tttccgcctg
    12961 atggtgggcg tttttgccgc tgaggttgtc gatgtgcagg gtcatcatgc tgtggttgac
    13021 gatgccgtgg aaaaattctt caaacaaatc gacatcgaaa cgtccgatta gtgcgcgggt
    13081 aaattcgatg ttgtacacga gtccggggcg gccggaaagg tcgatgacga cgcggctgag
    13141 ggcttcgtcg agcgggacgt aggaatgtcc gtaacggcgg atgccttttt tgtcgccgag
    13201 tgcctgccgg attgcttgtc cgagtgtgat gccgatgtct tcggcggtgt ggtggtcgtc
    13261 gatgtgcagg tcgcctttgc agctgatgtc gatgtcaatc atgccgtggc gggcgatttg
    13321 gtcgatcatg tgttcgagga agggaacgcc ggtatccagc ttgcttttgc cgctgccgtc
    13381 gaggttgatg gagacggtga tttgggtttc gcaggtattg cggttgacgg taacgctgcg
    13441 gcagccggca gcagatgcgg tttcggcaat tcgggtttcg gcagttccgg tgctttctgc
    13501 ggcggtttcg gggacagtgc gttcgcggtg ttttctgtcg agccagcctt tgggtttgcc
    13561 ggtgtgtttt tcgagttttg ccatcaggct gggacggatg ccgcgcgcat cggggtcttc
    13621 tgcctgcttt tcaaggcgtt gtttgagttt gtaaagtgcg acgggggtac ggtagccgca
    13681 gagtttggca agcttgggca gcgaacctgc ttcttgggcg agagtcagga agttgttcag
    13741 gtggagttgt gtcttggtca tagggattcc gtttggttta tcggtagagc cggcggataa
    13801 cgtcgaggac ggcatcgttt tgtgcggagc tgcctatggt aatgcgcagg cagtgttcca
    13861 aaagcggatg tgcgccgtgc agttttttga cgaggatgcg gttttgtttg agtgtgtcaa
    13921 acaacaaatc ggcatcgggt acgcgtatgg taatgaagtt tgcctgactt ggaaaggcgt
    13981 tcagacggca tattttgccc aattcggcga acatccgttc gcgttcgttt ttcaggctgt
    14041 cgatgttggc agaaatgatg ccgtagtgtt gcagggcgag tttggcggtg gtcaggctca
    14101 gttggttcat attgtagggc ggcaggattt tttgcagttc gccgatgact tcgggacagc
    14161 ctgtcgcata accgatacgc agtccggcaa aaccgatttt gctgagggtg cgtaagacta
    14221 tcaggttggg aatcctgccc gcctgcggca ggaagctgtc gccgttgaat gcgccgtagg
    14281 cttcgtcgac gacgacgatg ccgtctgaag cttcgatggc ggcttcgatt tcggcacgcg
    14341 tgaaacatac gccggtcggg ttgttcgggt aggcgataaa ggtcagggca gggcggtgct
    14401 tcctgacggc ttcgaggacg gcgggcaggt tgagggtgaa atctccgttc agtggaacgc
    14461 cgacataatc catgccgtac agcgcggcgt tgtggcggta catgacgaaa ccgggttcgg
    14521 ctgccaacat tgccgcgccg ggtttggcgg tcagcatggt gatgaactgt atcagttcgt
    14581 ccgaaccgtt gcctaaggcg acggcggcgc agtcagggat gtcgaacgcc gaacgcaatg
    14641 cttcctgcaa accgcagccg gaggggttgg gatagaggtg gatgggggcg gatgccagtt
    14701 gcgcccgcca ttcccgcatc agggcttcgt gcccctcaaa aggatgggcg gggctttcca
    14761 tcgcatcgag tttggcaaaa ccgggcggaa catcggtgat tttgtatgcg gacatggcga
    14821 ggatgtcgtt gcggatgaag gagcggacgg atttcatcgt gtttccttaa tggttggaat
    14881 atgcctgtac gccgtttcgg cattatttca tacggaactc tgccgcgcgt gcgtgggcgg
    14941 tcaggctttc gccgtgtgcc agcacgctgg cggtttcgcc taatttttgc gcgccctgtt
    15001 ccgaaacctg aatcaggctg gagcgttttt ggaaatcata tgtccccaaa ggcgaggaaa
    15061 agcgggcggt tcggctggtg ggcaacacat ggttcgggcc ggcgcagtag tcgccgaggc
    15121 tttcgccggt gtagcgtccc atgaaaatcg cgccggcatg gcggattttt ttcgcccatt
    15181 cctgcgggtt ttcgactgac agttccaagt gttcggggga aatgtagttg gagatttcgc
    15241 aggcttcgtt caagtcttta acgagtatca tcgcgcccct gttgccgagc gaggcctcga
    15301 tgatgtcgcg gcgcggcatg gtttcgatga gcctgtccat agcggcttct acttcgtcga
    15361 gatacgcttg cgacgtgccg atgaggatgg cttgggcaat ttcgtcgtgt tcggcctggc
    15421 tgaacaaatc catcgccacc caatcggcgg gtgtcgtgcc gtcggcgatg accagtattt
    15481 cagacggccc cgccaccatg tcgatgccga ccacgccgaa cacgcggcgt ttggcggcgg
    15541 cgacgaaggc gttgcccgga ccggtgattt tatcgacttg ggggatggtt tccgtgccgt
    15601 aggcgagggc ggcgatggct tgcgcgccgc cgacggtgaa gactttggtt acgccggcga
    15661 cgtatgcggc ggcaagcacg atgtcgttgc gttcgccttt cggtgtcggt acgaccataa
    15721 tgatttcttt cacgcctgcg acgtgggcgg gcatcgcgtt catgatgacg gaactcggat
    15781 atgccgcctt gccgcctggg acgtaaatgc cgacgcggtc aagcggggta atctgttgcc
    15841 ccagcagcgt gccgtcttca tcggtgtagc tccacgattc cattttttgg cgttggtggt
    15901 agctttcgac acggcgggcg gcggtctgca atgctgtctg aacgtcgttc ggaatgcgct
    15961 cgaacgccgc gttcaaatcg gcttgcgtga gtattaaatc atcgatgctt ttagcgtttg
    16021 tctgatcgaa tttgttggtg tattcaatca aagccgcatc gccgcgcttt tgcacgtcgg
    16081 cgcaaatgtc ggcgacgatg cgttcggttt cggggttttg cgcggtttca aaagccagca
    16141 gggctttgag tccggcttgg aaatcgggcg attgggtgtt gagttttttc atgatttggg
    16201 attctccttt gttattttgg gacttgggat cgtctgaagc gtctcttgct cggttttggg
    16261 tctgatatac gcttttcctc ccccgctacc aaacttcaat tcgggcaggc atttttgttg
    16321 cagaccgagt ttggctaata cctcttcgct acgagaggta gtacggaaac ggtaacgggt
    16381 ttgttttctg ccgtttttgg tttcctcagt cattgtttca aaaccgtcca cttcgtacgc
    16441 actgacaaca gcgttttgca gaccggtgtg aacgccgata acgtatttca cttttgaagc
    16501 aacatcttta ccgataaccc agttgcccaa cgtacgcgat ttgaggttgg catcgtcttg
    16561 gttgtcgaaa aggtagtcta attcttcgtc agtatctaaa tcgaaagcat tgtggatttt
    16621 gatggcgaga atcagcccgt cggggttaat ctcgctaagt gggagagaag agaatccaaa
    16681 gcggcgatct agttcttcta cgctaatacc acccggacca tgcccggcaa ttttattttt
    16741 gagtttttta cccaagacag atttaacaaa atgaattaag gcagattcgg cggcgagcgc
    16801 ttcgacttca gtcagatgat agctgatgat atagcgaccg agtttcttgc atttggagat
    16861 ggctttgagt ttccgatcga taatctcgcc ggagactggg tcttgtgaac gactagcaac
    16921 ccattcatgc tcgaagatac gattaccgca accttttccg atgtagagga ttttgtcttt
    16981 tttcaggtcg gtcagacaat agacataaaa ccgacgctcg ccgttattca aaacggaaag
    17041 ggtcgaatct gagaattttt ttatttttgc aaccatttta acttctccta tacagtttac
    17101 tgccggtgat attttcagac ggcctgtcgg cgggaacgga tagggtcgtc tgaaaacgca
    17161 tctttattca aatgaatgcc cacttcgcct tcactgcgcc gccgaacgac tgaataatcg
    17221 gctccagcag cgcgtatttc gttttcaaag cagccttgtt gaccaccagg tagctggaaa
    17281 tgtcgacgat gtgttcgact gcctccaagc cgtttgcctt caaggtgttt cccgtcgaaa
    17341 ccaagtccac aatcgcatcg ctcaagccga ccagcggcgc aagttccatc gagccgtaca
    17401 gtttgataat gtccacatgg acacccttgc cggcaaaatg agatgccgcg atttcaggat
    17461 acttcgtggc aatcttcaga cggcatccgg gttgcgaagc ggcttcgtaa tcaaaccctt
    17521 tacgcacagc aaccatcatg cggcacttgg caatctccaa atccaaaggt cggtaaagcc
    17581 ccgtgccgcc gtgttcgatc agcacgtctt tgcccgcaat gccgaagtcc gccgcgccgt
    17641 agcggacata agtcggcaca tcggttgcgc ggacaatgac aaggcggatg ttttcatggt
    17701 tcgtcccgat aatcagcttg cgcgattttt cgggctcttc agtcggagca atgccggcag
    17761 cggcaagcag cggcagcgtc tcttcaaaaa tgcgcccttt ggataaggcg atggtcaaag
    17821 cattatcctg catggtgtgg atgtcctttg tcggttgaaa caataatgcc gtctgaacag
    17881 gcaccggcgg tgcagacggc atacggtttt atcagagcag ggcccttaca tcggcagcaa
    17941 tcgtttccgg ctcgcttccg taaggcgaga aaatggcaac ctcaccgttt ttgtcgataa
    18001 gatacgcacc ggaagagtgg tcgaccaaat agttttcgct gtcgtctttt tgattgattt
    18061 tggcagaaac cacgcggtat tgctgcttga tgaccggcag gttttggccg cccgttgccg
    18121 tcagaccgat aaagtccgga ttgaactgtt tggcatactt gccgatgatt tcaggcgtgt
    18181 cgcgttccgg atcgatgctg acgaacacca ctttcacgtc cttagcctgc ccgcccaact
    18241 gcttcaaagt gtcgctgtac gtcaaaagct ctgtcgggca gacatcggga cagtgcgtaa
    18301 agccgaaaga cagaatcacg accttgcctt tcaaatcgct caggctgaaa ggcttgcctt
    18361 cgccgtcggt cagtgtgaaa tcgccgccga tgtcttcctt acgcatatcc gtaccgcgcg
    18421 tttgcggctt tgccgcattt tccgcagccg gcgcggatgc acttgaagaa gcggcttgcg
    18481 ccgcactgtt gtcttgaggt ttgcaggcgg caagcgcggc aagtacgaaa acgcccggca
    18541 aaaaggaacg cggtacggaa aacatgacaa tctcccaaga ataaatagaa aaacctgatt
    18601 cgtacggaat ttgaatattt ccatcttaac gcgtttggca gatttaatgt agtatttttt
    18661 tacgttgtct gtacggcagg aattgccgaa tcatacagaa tctttggagc ggaaagaaca
    18721 gtaagattaa aataataaaa attctattta aaatcaaaaa caaaataaaa acagataaaa
    18781 taatttcaaa aaaaggttta cagaatctca aaagccatta taatggcaat ttctttacca
    18841 gcccaggtgg cgaaattggt agacgcaggg gactcaaaat cccccgccgc aaggtgtgtc
    18901 ggttcgagcc cgaccctggg caccacaacc gcttttggag cggttatttt tttgtctatt
    18961 gctgtttaat gaaatagata agactaaata tagcggatta aatttaaacc ggtacggcgt
    19021 tgcctcgcct tgccgtacca tctgtactgt ctgcggcttc gtcgttttgt cctgatttaa
    19081 atttaatcca ctatacaatc aatatattta agtcgttgtt agccctcttg gttctgtcaa
    19141 tgccgtccaa tacagtccgg tgcaatgagc cattttcagg aagtatggtt gggaatattt
    19201 ttgatatatc aaaaataaaa tcaagttcaa tcaatatccc gagtctttat tgggatgaaa
    19261 ccctgtaaat tttaaaattc ttgatacttt cacatctgcc cccatgcaaa ctgtcaacaa
    19321 tttccatctt tcaaaaaagg tactcctgat tttattcttt taaaatcaat caataatttc
    19381 tccgctttca aaaaaaattt ctccgctttc aaaaaatatt aaaagatgaa agatttttct
    19441 tgattgaact gtcggtttgc gttatcgttt ttactctttt tcacaaactc tgtgttcctt
    19501 tccaattgat tggataatct gtctgttgcc gtgttccccc aatgcgcgta ccctaaatcg
    19561 ctgcttggtg gaattgcatt acaggtgctg tggcaaagcg gtttgtccct attggtttga
    19621 aaccgtataa aagaggtcgt tatgcagtta tcaggcgcgc aaatcatagt gcagagtctc
    19681 aaagccgaag gcgtcgagta cgttttcggt tatcccggcg gtgcggttat cgaaatctac
    19741 gatgccctgt tccaactcaa taaattcaaa cacattctta cccgccatga gcaggcggca
    19801 gtacacgcgg cagatgcgta tgcgcgcgtc agcggtaagg tgggcgtggc attggttaca
    19861 tccggcccgg gcgttaccaa tgcgctgacc ggtattgcta ctgcctatac ggattcgatt
    19921 ccgatggtgg tcatcagcgg acaggtaggc aattccctga ttggtacgga tgcgttccaa
    19981 gaagttgata cggtgggtat tacccgtccg tgcgtcaaac acaatttctt ggttacggac
    20041 atcaatgagt tggtggaaac cattaaaaag gcgttccaaa ttgccgcaag cggccgaccg
    20101 gggcccgtgg tgcttgatgt cccgaaagat gttacgcagg cgatggcgaa attcagctat
    20161 cctcaggaag acatttttat ccgttcgtat caaccggttg ttcaagggca tatcggtcag
    20221 attaaaaagg ccgtgcagat gttggcatct gccaaacgcc cggtcgtcta ttttggcggc
    20281 ggcgtggtgt tgggtaatgc ttctgaagag ctgacccgat ttgtccgaat gacgggtgct
    20341 ccgtgcacgg gtacgctgat gggcttgggc gcttatcctt ccggcgaccg ccaattcctc
    20401 ggtatgctcg gtatgcacgg tacttacgag gcaaaccttg ccatgcagaa tgcggatgtc
    20461 gttcttgccg taggtgcgcg ttttgacgac cgtgtggttt ccgtaccgtc caaatttttc
    20521 gagaaggcga agaaggttat ccatatcgat gtcgatcctt ccagcatcgc caaacgcgtg
    20581 aaggcggaca ttccgattgt cggcgacgtg aaaaacattt tgtccgagat ggttgcgctg
    20641 tggcaaaaac aagagtccgt gccgtctgaa gatgctttgg gcaaatggtg gaaaaccata
    20701 gaggaatggc gttcccgcga ttgcttgtgg tttgacaacg gcagcgaaat tatcaagccc
    20761 caatatgtga ttcagaagct tgccgagatt accggcaatt cggcaatcat cacatcggat
    20821 gtagggcagc atcaaatgtt tgcggctcaa tattatcctt tcgaacgtcc gcgccaatgg
    20881 ctcaactccg gcggtttggg tacgatgggc gtgggtctgc cttatgcgat tggtgcaaaa
    20941 cttgccgccc cggatcaaga cgtattctgt attaccggcg acggctcgat ccagatgaac
    21001 atccaagagt tgtccacctg tttccaatat cggattccgg ttaacgtcat tacgctgaac
    21061 aacggttatc tgggtatggt acgccagtgg caggaaatat attacggcgg tcgagagtcg
    21121 gaaacctatt tcgattcttt gcccgatttc gtcaaacttg ccgaggcata cggccatatc
    21181 ggcatccgcg tggacaataa atctgatgtg gaaggtgcct tgttggaagc attgaaccaa
    21241 aaagacaggc tggtgtttat cgacttcctg accgaccaga aacagaatgt gatgcctatg
    21301 gtcggcaacg gcaaaggttt ggacgaaatg gtacttccgc cgcatatgcg tacggacgga
    21361 aaggcgtaag gagaggcaaa tgcgacatat cttatctgtt ctgatcgaaa acgaatcagg
    21421 tgcgatgagc cgcgtggtcg gtttgttctc tgcacgcgat tacaatatcg attctttggc
    21481 ggttgcgccg accgaagaca aaaccctgtc acggatgacc atcgttaccc acggcgacga
    21541 gcaggttatc gaacaaatca ccaagcaact caataaattg attgaagtga ttaaagtggt
    21601 cgatttgaac gaaagccgtt ttgtcgaacg cgaactgatg ttggtaaaag tccgtgccgc
    21661 cggcaaagac cgcgacgaat ttttacgctt gaccgaaatc taccggggca gcatcatcga
    21721 cgtaaccgac cgcagctata cgattgaaat taccggctcg accgacaagc tggattcctt
    21781 tttggaaacg gtcggacgcg cccaaatttt ggaaaccgta cgtacaggtg cggccggcat
    21841 cggtcgcggt gagcgtattt tgaaaattta acgccgtaac ctttcagacg gcatggtatt
    21901 tgaatgccgt ctgaaaaacg aacggcagga gagatttatg tcgaacatta aaattgtcgc
    21961 actggttacc gtcaaaccgg aatacacgga aacactggca gcacagttta aagaactggt
    22021 caaagccagc cgtgcggaag agggcaacat cagctacaat ctccatcagg aaatcggcaa
    22081 accgaaccgt tttgttttcg tggaaaattg gaaatcccaa gcagctattg acgaacataa
    22141 tgccagcgcg catttccaag ccttcgtcca gtccgtcgac ggcaaaaccg aagcgttgga
    22201 aatcgtattg atgaatgaag ttgccgttta agcggcacac tctgtttaac ccgtccgaag
    22261 ccgctcaaca ttttaaggct tcgacaacca tttaccttaa aggaaatcaa atgcaagtct
    22321 attacgataa agatgccgat ctgtccctga tcaaaggcaa aaccgttgcc atcatcggtt
    22381 acggttcgca aggtcatgcc catgctgcca acctgaaaga ttcgggtgta aacgtggtga
    22441 tcggtctgcg ccacggctct tcttggaaaa aagccgaagc agccggccat gttgttaaaa
    22501 ccgttgctga agcgaccaaa gaagccgatg tcgttatgct gctgctgcct gacgaaacca
    22561 tgcctgccgt ctatcacgcc gaagttgcag ccaatttgaa agaaggcgcg acgctggcat
    22621 ttgcacacgg cttcaacgtg cactacaacc aaatcgttcc gcgtgccgac ttggacgtga
    22681 ttatggttgc ccccaaaggt ccgggtcata ccgtacgcag tgaatacaaa cgcggcggcg
    22741 gcgtgccttc tctgattgcc gtttaccaag acaattccgg caaggccaaa gacatcgccc
    22801 tgtcttatgc ggctgccaac ggcggcacca aaggcggtgt gattgaaacc actttccgcg
    22861 aagaaaccga aaccgatctg ttcggcgaac aagccgtatt gtgcggtggc gtggccgagt
    22921 tgatcaaagc aggttttgaa accctgaccg aagccggtta cgcgcctgaa atggcttact
    22981 tcgaatgcct gcacgaaatg aaactgatcg ttgacctgat tttcgaaggc ggtattgcca
    23041 atatgaacta ctccatttcc aacaatgcgg agtacggcga atacgttacc ggccctgaag
    23101 tggtcaatgc ttccagcaaa gaagccatgc gcaatgccct gaaacgcatc caaaccggcg
    23161 aatacgcaaa aatgtttatc caagagggta atgtcaacta cgcgtctatg actgcccgcc
    23221 gccgtttgaa tgccgaccac caagttgaaa aagtcggcgc acgactgcgt gccatgatgc
    23281 cttggattac ggccaacaaa ttggttgacc aagacaaaaa ctgattgttt tcaaacggga
    23341 ctgcctacac atcgtgtagg cagtttgtta tatggatacc gtctgaacat cgtgttcgga
    23401 cggtatctgt gttgcggatg aatttaaaca ggcacagttc tgtcagtcgc ccagagccgc
    23461 gaccattacc gctttaatcg tgtgcatacg gttttccgcc tgatcgaaca cgatgccggc
    23521 cggactttcg aatacttctt ctgtaacttc cacaccgttc agcccgaagg tttcgtaaat
    23581 ccattcgccg actttggttt cgcggttgtg gaaggcgggc aggcagtgca tgaatttgac
    23641 ttgcggattg cccgatgccg ccatcagttc gggcgtaacg cggtaatctt tcagcaaatc
    23701 gatgcgttcc tgccagactt ctttcggctc gcccatgctg acccatacgt cagtatgaat
    23761 gaaaccgaca cctttgacgg cttcatgcgc gttttcggtc agggtaattt ttgcaccggt
    23821 ttctttggcg gcggcgtgtg cggcggcgat aatgccttca gacggccaca ggctttgagg
    23881 tgcgccgata cgcacgtcca tccccaattt tgcccctaaa atcagcaggg aattgcccat
    23941 gttgtaacgc gcgtcgccga cgtaggcaaa cgcggtttgg ttcaaaggtt tgccgctgtg
    24001 ttcgcgcata gtcagtgcgt cggcaagcat ttgtgtggga tggaactcgt tggtcagccc
    24061 gttgaacacg ggtacgcccg catattttgc caattcttcg acagtttcct gagcgaagcc
    24121 gcgatattcg atggcatcgt acattctgcc taagacgcgc gccgtgtctt tgatgctttc
    24181 tttgtgcccg atttggctgg cggacggttc cagataggtt gcatccgcgc cttggtcgcg
    24241 tgcggcgact tcaaacgcac agcgtgtgcg cgtggatgtt ttttcaaaaa tcagggcgat
    24301 gtttttccct ttcatccgct gaatctcgcg ccctgccttt ttggcgtctt tcaactcggc
    24361 ggcaaggtcg aggtaggcgg tgatttcttc cggcgtgaag tccaaaagtt tcagaaaatg
    24421 gcggtttttc aggttcactg tcgtttcctt tatgtggggg cgtgaacgcc gtatttgcag
    24481 cgtttgcgga aagaagggtt agcggaatgt tgcaatgccg tctgaaggtt gttcagacgg
    24541 cattgcggtc aatcccttac tccatacgga tgacggggat caaacattca cggtctcggc
    24601 tacttcgttg tagctgtcga tttcgttgaa gttcatatag cggtagattt tatcgccctg
    24661 ttcgttgatg atgccgatat tggcttgata ttcttcaacg gtcgggattt tacccagttt
    24721 ggagcaaatc gccgccaact ctgccgagcc gaggtaaacg aaggtgtttt tacccaagcg
    24781 gttcgggaag ttgcgggtcg aagtggacat gacagttgca ccttcgcgta cttgggcttg
    24841 gttacccata cacaacgaac agcccggcat ttccatacgc gcgccggcgc ggccgagtac
    24901 gccgtagtga ccttcgtcgg acaactcttt cgcgtccatt ttggtcggcg gcgctaccca
    24961 caggcggacg gggatgtcgc tcttgccttc caaaagtttg gaggcggcgc ggaagtggcc
    25021 gatgttggtc atacaagaac cgatgaacac ttcgtcgatt ttggtgccgg agcgttcgga
    25081 cataaagcat acgtcgtccg ggtcgttcgg gcaggcgata atcggctctt tgatgtcgtc
    25141 catgttgatt tcaatcacgg cggcgtattc ggcatcttta tccgcttcga gcaactcggg
    25201 atttgccagc catttttcca tagctttgat gcggcgttcc aaagcgcgcg gatcttgata
    25261 gccgttggca atcatatttt tcatcaacac gacgttggat ttcatgtact cgataatcgg
    25321 ctctttattg agcttcacgg tacagccggc ggcggagcgt tcggcggatg cgtcggtcaa
    25381 ttcaaaggct tgttccactt tcaaatcggg caggccttcg atttcgagga tgcggccgga
    25441 gaagatgttt ttcttaccgg ctttggcaac ggtcagcaaa ccttgtttaa tcgcgtaaag
    25501 cgggatggcg ttcaccaaat cgcgcagggt tacgcccggt tgcagcttgc cgctgaaacg
    25561 taccaatacg gactcgggca tatcgagcgg tattacgccc gttgcggcgg caaaagcgac
    25621 caagcctgaa cctgcgggga aggaaatacc gatggggaaa cgggtgtggc tgtcgccgcc
    25681 tgtaccgact gtatcaggca acagcaggcg gttgagccac gagtggatga cgccgtcgcc
    25741 cggacgcagg gacacgccgc cacgggtaga aataaaggcg ggcagttctt tatgggtttt
    25801 tacatcgaca ggtttcggat aggcagcggt gtggcagaaa gactgcatca ccatatcggc
    25861 ggagaagccc aaacaagcca agtctttcaa ctcgtcgcgg gtcatcggcc cggtcgtatc
    25921 ttgcgagccg accgtcgtca tgcgcggttc gcagtaagta cccggacgca cgccttgtcc
    25981 ttcgggcaga ccgcaggcgc gaccgaccat tttttgcgcc aaggtgaaac cgactttgct
    26041 ttcggcaggc gcttgcggca ggcggaatgc agtagaggca ggcagtttca gggcttcgcg
    26101 cgctttggcg gtcagaccgc gaccgataat caggttgata cggccgccgg cttgcacttc
    26161 gtccagcaat acttgtgatt tcaattcaaa ctcggcaaca gtctcgccgt ttttcacgat
    26221 tttaccttca taaggaagga tattgacgac atcgcccatt ttcagcgcgg aaacatcgac
    26281 ttcaatcggc agcgcgccgg agtcttcttg agtgttgaag aaaatcggcg cgattttacc
    26341 gcccaagcac acgccgccga agcgtttgtt cggcacgaac gggatgtctt cgccggtatg
    26401 ccaaatgacg gagttggtcg cggatttgcg tgaagaaccg gtaccgacca cgtcaccgac
    26461 gtaggcaacc ggatggcctt tggctttgag ttcttccaac aatttaatcg gaccgacttc
    26521 gcccggtttg tcgggcgtga tgccgtcgcg cgggtttttc agcatggcca gcgcgtgcag
    26581 cggaatatcg ggacgactcc acgcgtcggg cgcgggagag aggtcgtctg tattggtttc
    26641 gccgtcaact ttgaagacgg taacggtgat tttttcggga actttggcac gggaggcgaa
    26701 ccattcggca tctgcccaag attgcaaaac ttcctgcgcg tatttgttgc ctttttcggc
    26761 tttttcctga acgtcgtgga aggaatcgaa catcagaagc gtatgtttca agcctttggc
    26821 ggcaatgggc gcgagtttgt cgtcgtccaa gagttcgatt aaggcgtgga tattgtaacc
    26881 gccgagcatc gtacctaaga gttcggtcgc atatttgggg gaaaccagcg ggctggatgc
    26941 gctgccttcg gcaacggcag ccaggaatga ggctttgact ttggcggcat cgtccacacc
    27001 gggcggaaca cggtgggcaa gcagctcgac caagaactcg ccttcgcctg cgggcgggtt
    27061 tttcagcagc tcaaccaaat cggcggtttg ctgcgcattc aaagggaggg cgggaatgcc
    27121 gagggcggcg cgctcggcgg cggctttacg ataggcttct aacatctctt tgttcctttt
    27181 tccgtttttc ttttgtcggg ttgcaaatga tctgcgttaa atattgtaaa caacatttgt
    27241 accgctatca tagactagtt ttaacaaaaa tggaactgtt atgacaggta atgtatagtg
    27301 gattaaattt aaatcaggac aaggcggcga agccgcagac agtacaaata gtacggcaag
    27361 gcgaggcaac gccgtaccgg tttaaattta atccactata atagggaacg gctatatgtc
    27421 tgcgggaatg tgtcaatgaa aacaaatgcc gtctgaagag aggttcggac ggcatttgtt
    27481 caacgttttt cctgtttgcg tatcagccaa acagcgagca aggcgaaggc tatggtaggc
    27541 agtgcgccgg cgaggaaggg tggggtgccg tagagttggc tggtaaaccc gaagagcctg
    27601 ccggcaaggt ggaacagcaa tccgagacag atgccgccga agagttttaa gcccatattg
    27661 ccgtggcgcg tggtttgcgg cgtaaaggcg aaggcaacga gcgccatgac ccatgcggcg
    27721 acggggtaaa cgagtttacg ccaccatgcg atggcgtaga tttgggtgtt ttggctgttg
    27781 ttttggaggt ggcggatgta ggtggtcagc tcgccgacgg acatttggtc gggcttgacg
    27841 agcaatacgt ccatcaggtt gcgtctgacg gcaatcggcc aagtttcttc ggcggcggcg
    27901 gatgtttcga ttttgtctgt acccatgatg ctgcggcgga tgtttttcaa ctgccagctg
    27961 ccgtcgctgt tcaaaacggc ggaatcggct tccactgcct ctgccaattc gtttttatcg
    28021 ttgcgcgccc aaattttgat gcccaaaagc gtatggtcgg gcaacattcc gcgcacattg
    28081 ataatgctgt ttttttcttt cagccaaagg ccggtatggc cggtgctgat tttgccgtta
    28141 atggcggcgg ctttgatgtt ttcggctttt tggctcagcg tgggcgcaac ccattcgccg
    28201 agcgcgacgg cggcaatagc aaaaataaaa ccgaactgag acagaatcaa cagcagcttt
    28261 ttggtgctca tgccgctggc tttgatgacg gccagttcgc tgccggcggc aagctggctg
    28321 agagaggcca gtccgccgat gaggacggcg aggggcatga gttcgtaggc gcgggcgggc
    28381 attttgaggg cggtgtagcc cagcatttcc catatgccgt aactgccttt gccgaggttg
    28441 ccggtttcgt acaggatttc aaaaaagctg tacaaagcga ggaaggcaag gagcgcgtaa
    28501 accgccataa ccgccatttg gcggatgatg taacgtgaaa tcaggttcat tttccgcctt
    28561 tcaatgtcag acttttgcca accgcctgcc agaagggctg gctgggcata ctgcggacgc
    28621 gcagaagtac gattgcgatg acgaacatga tgatgtgcat aggcagcagt ccgagccaaa
    28681 aatggatttt gccgtcttcc acggcttcaa aaagcagggt cagcccgttt tggtaaatta
    28741 aaaacaaacc gatggcaatc aagatattgt aggtatgtcc gctgcgcggg ttgaaatagg
    28801 aaagcggcac ggcgagtagg cagagcagga ggacgctgac ggtcagcgag atgcgccaca
    28861 tcaattctgc ctgatgttgc ggattgctgc tgccaatcag ttgggcggtc gaaatggtgc
    28921 ggcggtggga aacggggtcg ataagtttgg gcgtggtgct gataatcagg ttgagttttt
    28981 ggaaggaaac ctgattgtag tcggcgcgtc cgggcgtgcc gctgtaacgg tagccgtggc
    29041 gcaattcgag cgtgcgtttg ttgtcgttca gcgagaagtt accttctttg gcgaagatga
    29101 tgttgtcgcc gccgtttttg tcctgttcgc gcaggaacag gtttttcatg atgccggatt
    29161 cggtgtcaaa ggtttcgacg aaataaaccc tgccgttgcg cttgcccaag ttattgaact
    29221 cgccggcttc caccaaagac aattcctgct tctgtttcaa aatttcggca tattcgcggc
    29281 tgcgcagttc cgcccacggt atcacccaaa gctgcatgac ggcaatcagg atggcaaacg
    29341 gcacggcaaa ctgcatgacg gggcgtatcc actgtttcaa cgccaatccg caggatagcc
    29401 agaccgacat ttcgctgtcg cgccagtagc gggtcaatac ggtcagcgtg ctgatgaatg
    29461 cggtcaacac cagcaaaagc ggggtcatac cgatgaccca gaagccgact aaggccaaca
    29521 cggcatcgat ggcgacacgc ccgtcagctg cgcggccaag caggttgatc gcctgcgtgg
    29581 acaccaacac cgccaagagg acgacgaaaa tgccgacggc ggtaaaagag agttctttga
    29641 tgaggtttct ttgataaatc ataaaatcgc ggtcaggggg gcggtaagcg gttcggcgca
    29701 ggcggattgt ccgcgcaagg tttcagacgg tgcttcaaaa aatggggtac aatcgtgcca
    29761 gccgtcttca gacggtcttt tgagcgaaag gccgtctgaa aactaccgac aaccgtctca
    29821 atcaggagaa taaacgtgga atttagcaca aaaaccgaaa tcttgcagga acagcaggca
    29881 ggcgcgcagt tatttgtctg tgccgacaaa gcacccgagc acaacaccgc cgcacatgcg
    29941 ctcttctctg ctttggaaga gggtcagaat ttttccgaca ccaaaatccc gacggacaac
    30001 ggtttgcagg cagtcgccgt cgtccgcctc gaaaaaaccg accgcgccgc gctgaacaaa
    30061 gccgccgccg aagccgccaa atgggcgcaa aatcaagaaa cggtcaatgt ggacgttcac
    30121 gcctttgatg aggcgcaagc agcagccgtt gccgaagctt ttgccatcgc gttcggcaac
    30181 gctgcatatc gtttcgaccg ctacaaaaaa gaagccaagc ccgccaaatt ttcgcaagcc
    30241 gtgttccaca gcgcacacga agccgccgtc aaagaagccc tgcgcgttgc cgaagcacaa
    30301 gtttacggac aaagcctctg ccgcgactta ggcaatgccg caccgaacga atgcacgcct
    30361 gagttcctcg cgcgtaccgc caaagccgaa gccgagaaac tgggcgcgca cgccaaaatc
    30421 atcgaaaaag actacatcaa agaaaacatg ggttcgtttt ggtcggttgc caaaggcagc
    30481 gtcgaagacc catatttggt tgaactgagc tatttcggtg cggccgacaa agaagccgcg
    30541 cctgtggtat tggtcggcaa aggcattacc ttcgacaccg gcggcatttc cctcaaaccc
    30601 ggcctgaaca tggacgaaat gaagtttgac atgtgcggcg cggcaaccgt catcagcacc
    30661 ttctgcgccg ccgtcaaact gcaactgccg atcaatctga ttgccatcgt cgccacttgt
    30721 gaaaacatgc cttccggtgc ggcaaacaaa ccgggcgatg ttgtgaaaag tatgaaaggg
    30781 ctgacgattg aagtgttgaa caccgatgcc gaaggccgtc tgattttgtg cgacgcgctc
    30841 acttacgccg agcagttcaa acccaaagcc gtcatcgatg tcgccaccct gaccggcgcg
    30901 tgcatcgtcg ccttggggca cgatgtcagc ggcgtgatgg gcaataatca ggatttgatc
    30961 gacagcctgc ttgccgcttc ctacaacgtg gacgacaaag cgtggcaact gccgctcttt
    31021 gaaacctaca aagaccagct caaatccaac tttgccgaca tccccaatat cggcacgccc
    31081 ggcgcgggca cgattaccgc cgcaacattc ctgtcctatt tcaccgaagg ctacccgtgg
    31141 gcgcaccttg acatcgcggg tacggcgtgg aaatccggag cggaaaaagg tgcgaccggc
    31201 cgccccgtac cgctgctgat gaactatctg cggaatcttt aattttgcca caatgccgtc
    31261 tgaagctttt cagacggcat catcctgtca aatgctcaaa caatatgccg aaagttacct
    31321 tttataccca tgttgaccaa atcccccttt ttacctgtcg gttgattgcg cgcgctatcc
    31381 gagacggcgg caggatactg gtgtggtccg actcgttcgg gcggcttcag gaattggaca
    31441 aaatgctttg gcaatacgag gccgagagtt ttattccgca cgaaatttgg gaaacggaag
    31501 aagccatgcc gtctgacaca tccgtcctgc ttgcctgcgg cggcaatctg ccccgaattc
    31561 ccgaaggcat ggccgttttg aacctgtccg acggtttttg gaacaccgct tcggtcttgc
    31621 ccgcgcgcgt tttggaaatc gtcggcaaca gcctggaaga tctcgccgac gcgcgcgaac
    31681 gctttaccgc ctaccgccga agcggttttg ccatcgaaca tcacggcatg gagggcaagg
    31741 catgaaatgc cgtctgaaat gccgcattta caaaatttaa acaggcttga ttctatttga
    31801 ttaaaatttc cttatcggtt gaaccccgcc acttggacat ctgtccttcg gggcggtaga
    31861 atcaaacctt attttggaag gttcaatccc ttccaaaaca gggcaacaca cagattgacg
    31921 ctttatgtgc catcctgtgt gttgaaacat tcaaactcgg ctataatccc gtttttccga
    31981 ctttatcgac agcgaagatc catcatgaac accattttca aaatcagcgc actgaccctt
    32041 tccgccgctt tggcgctttc cgcctgcggc aaaaaagaag ccgcccccgc atctgcatcc
    32101 gaacctgccg ccgcttctgc cgcgcagggc gacacctctt caatcggcgg cacgatgcag
    32161 caggcaagct atgcgatggg cgtggacatc ggacgctccc tgaaacaaat gaaggaacag
    32221 ggcgcggaaa tcgatttgaa agtctttacc gatgccatgc aggcagtgta tgacggcaaa
    32281 gaaatcaaaa tgaccgaaga gcaggcccag gaagtgatga tgaaattcct gcaggagcag
    32341 caggctaaag ccgtagaaaa acacaaggcg gatgcgaagg ccaacaaaga aaaaggcgaa
    32401 gccttcctga aggaaaatgc cgccaaagac ggcgtgaaga ccactgcttc cggtctgcag
    32461 tacaaaatca ccaaacaggg taaaggcaaa cagccgacaa aagacgacat cgttaccgtg
    32521 gaatacgaag gccgcctgat tgacggtacc gtattcgaca gcagcaaagc caacggcggc
    32581 ccggccacct tccctttgag ccaagtgatt ccgggttgga ccgaaggcgt acggcttctg
    32641 aaagaaggcg gcgaagccac gttctacatc ccgtccaacc ttgcctaccg cgaacagggt
    32701 gcgggcgaaa aaatcggtcc gaacgccact ttggtatttg acgtgaaact ggtcaaaatc
    32761 ggcgcacccg aaaacgcgcc cgccaagcag ccggatcaag tcgacatcaa aaaagtaaat
    32821 taagtccgaa tccatgcccg aaacaggttt tcgggcattt ttacggcaaa tgccgtctga
    32881 agccaccaaa cagcggttca gacggcatcc ctttcaaaag cgcaccatta tgaaaaacat
    32941 cgtcatcctg atttccggac gcggcagcaa tatgcaggca atcgtcaatg ccgccattcc
    33001 caacgtccgc attgccgccg tgttgagcaa cagcgaaacg gctgccggtt tgcaatgggc
    33061 ggccgaacgc ggcatcccga ccgacagcct gaatcataaa aactttgaat cacgacttgc
    33121 cttcgatacg gccatgatgg aaaaaatcga cgcatatcaa cccgatttgg tggttttggc
    33181 aggttttatg cggattctga cccctgagtt ctgcgcccat tacgaaggca ggctgatgaa
    33241 catccatccg tccatcctgc cctcgttcac cgggcttcat acgcacgaac gcgctttgga
    33301 agcaggttgc cgcgttgccg gctgtacgat tcatttcgtt acggccgagc tggattgcgg
    33361 cccgattgta tcgcaaggga ttgtgccgat acttgacggc gatacggcag acgatgttgc
    33421 cgcccgggtt ttggctgtcg aacacaaact ctatccgaaa gccgttgccg atgttgccgc
    33481 cggccgcctg attatcgagg gaaaccgtgt cagaaattcg gaaaacgccg atgccgcccg
    33541 tttcctgacg gcgtaaaccg ggcgggagca aatgatgaag acttttaaaa atatattttc
    33601 cgccgccatt ttgtccgccg ccctgccgtg cgcgtatgcg gcaaggctac cccaatccgc
    33661 cgtgctgcac tattccggca gctacggcat tcccgccacg atgacatttg aacgcagcgg
    33721 caatgcttac aaaatcgttt cgacgattaa agtgccgcta tacaatatcc gtttcgaatc
    33781 cggcggtacg gttgtcggca ataccctgca ccctgcctac tataaagaca tacgcagggg
    33841 caaactgtat gcggaagcca aattcgccga cggcagcgta acctacggca aagcgggcga
    33901 gagcaaaacc gagcaaagcc ccaaggctat ggatttgttc acgcttgcct ggcagttggc
    33961 ggcaaatgac gcgaaactcc ccccgggtct gaaaatcacc aacggcaaaa aactttattc
    34021 cgtcggcggc ctgaataagg cgggtacggg aaaatacagc ataggcggcg tggaaaccga
    34081 agtcgtcaaa tatcgggtgc ggcgcggcga cgatacggta acgtatttct tcgcaccgtc
    34141 cctgaacaat attccggcac aaatcggcta taccgacgac ggcaaaacct atacgctgaa
    34201 gctcaaatcg gtgcagatca acggacaggc cgccaaaccg taaatcggtt ttcaggcggt
    34261 tttttgccgt tttgtccata tttctgtata agccgcttga aattgtttac aataacgcca
    34321 tatgcaaagc ggttttaacg ctattttcgg gaatgacacc atgcaggtta catcaaaatg
    34381 gatagacgga atgtgctttg tcggcacgtc ggaaggcggg cacagcgtcg ttatggaggg
    34441 atcggcggca gaaggcgcgg ccaagcgcgg gcccagccct ttggaaatgc tgctgttggg
    34501 ggtggcgggc tgttcgagca tcgatgtggt gatgattgcc gaaaaacagc gtcagaaagt
    34561 gactgactgc cgtgcgacgg ttacggcgaa acgggcggac gatgcgccgc gcgtgtttac
    34621 cgaaatccac atccatttca aggtaatcgg gcatgatttg aaagaatcgg ccattgagcg
    34681 cgccgttcag atgtctgccg aaaaatactg ttcggcttcg attatgttgg gcaaagcggc
    34741 aaagattacc cacagttttg aaattgccgg ggcggagaaa taggcatcgg gcggcattat
    34801 ggggaaacgg ctgatgccgt ctgaagggga aaggtgcggc aggcaatccg gaggcgcata
    34861 gaaaagcaga tatattcgga ctgcacctcc cgaatatatc tgcctgctgt ttcctcttta
    34921 ttcagccttt ataatacttg gacttgtcgg ggtattgtgc aggcttgatt ccggattgtc
    34981 aacaattttc ggtcaaattt taaatgccgc gttttaaaat gatgcccgcc tgattttgcg
    35041 ggcgggcgga aggcgggaat cccgttattt taagccatat attaatttat tgaattaaaa
//
LOCUS       DAXFZR010000012        32471 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000012 DAXFZR010000000
VERSION     DAXFZR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32471
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.012"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(185..982)
                     /gene="truA"
                     /locus_tag="ACFJAS_000480"
     CDS             complement(185..982)
                     /gene="truA"
                     /locus_tag="ACFJAS_000480"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC3981813.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQTA
                     PGFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAALLIGEQDFS
                     SFRAAGCQAKSPVKTIYRAGLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIIRPQIPEWL"
     gene            1673..1798
                     /locus_tag="ACFJAS_000481"
                     /pseudo
     CDS             1673..1798
                     /locus_tag="ACFJAS_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1848..2312)
                     /locus_tag="ACFJAS_000482"
     CDS             complement(1848..2312)
                     /locus_tag="ACFJAS_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981814.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGAGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDGRLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(2389..5226)
                     /locus_tag="ACFJAS_000483"
     CDS             complement(2389..5226)
                     /locus_tag="ACFJAS_000483"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC3981815.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVAEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(5331..6590)
                     /locus_tag="ACFJAS_000484"
     CDS             complement(5331..6590)
                     /locus_tag="ACFJAS_000484"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC3981816.1"
                     /translation="MKVLVLGAGVAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(6669..8060)
                     /locus_tag="ACFJAS_000485"
     CDS             complement(6669..8060)
                     /locus_tag="ACFJAS_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3981817.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGAGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            8260..8406
                     /locus_tag="ACFJAS_000486"
     CDS             8260..8406
                     /locus_tag="ACFJAS_000486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981818.1"
                     /translation="MGMWRAAEIINAADCHTSEIYSGLNLNRYSVASPCRTSCTVCGF
                     VALS"
     gene            complement(8523..9299)
                     /gene="lpxA"
                     /locus_tag="ACFJAS_000487"
     CDS             complement(8523..9299)
                     /gene="lpxA"
                     /locus_tag="ACFJAS_000487"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC3981819.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVRIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(9342..10028)
                     /locus_tag="ACFJAS_000488"
     CDS             complement(9342..10028)
                     /locus_tag="ACFJAS_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC3981820.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFSFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQV
                     IAVCDEIDKL"
     gene            complement(10235..10684)
                     /gene="fabZ"
                     /locus_tag="ACFJAS_000489"
     CDS             complement(10235..10684)
                     /gene="fabZ"
                     /locus_tag="ACFJAS_000489"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC3981821.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(10720..11763)
                     /gene="lpxD"
                     /locus_tag="ACFJAS_000490"
     CDS             complement(10720..11763)
                     /gene="lpxD"
                     /locus_tag="ACFJAS_000490"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC3981822.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSEHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(11796..12296)
                     /locus_tag="ACFJAS_000491"
     CDS             complement(11796..12296)
                     /locus_tag="ACFJAS_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC3981823.1"
                     /translation="MIRLTRAFAATLIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(12362..14740)
                     /gene="bamA"
                     /locus_tag="ACFJAS_000492"
     CDS             complement(12362..14740)
                     /gene="bamA"
                     /locus_tag="ACFJAS_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC3981824.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(14797..16137)
                     /gene="rseP"
                     /locus_tag="ACFJAS_000493"
     CDS             complement(14797..16137)
                     /gene="rseP"
                     /locus_tag="ACFJAS_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC3981825.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(16172..17407)
                     /gene="ispC"
                     /locus_tag="ACFJAS_000494"
     CDS             complement(16172..17407)
                     /gene="ispC"
                     /locus_tag="ACFJAS_000494"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC3981826.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(17412..18209)
                     /locus_tag="ACFJAS_000495"
     CDS             complement(17412..18209)
                     /locus_tag="ACFJAS_000495"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC3981827.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(18212..18958)
                     /locus_tag="ACFJAS_000496"
     CDS             complement(18212..18958)
                     /locus_tag="ACFJAS_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC3981828.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(19014..19571)
                     /gene="frr"
                     /locus_tag="ACFJAS_000497"
     CDS             complement(19014..19571)
                     /gene="frr"
                     /locus_tag="ACFJAS_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC3981829.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(19713..20705)
                     /locus_tag="ACFJAS_000498"
     CDS             complement(19713..20705)
                     /locus_tag="ACFJAS_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3981830.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDGCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(20710..21114)
                     /locus_tag="ACFJAS_000499"
     CDS             complement(20710..21114)
                     /locus_tag="ACFJAS_000499"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC3981831.1"
                     /translation="MVYGIDYCADAECYLKIKNQIKEGIGNIGGIQFAETKELGRVNR
                     IDPLNITYLRVRGMWGIENPWFVFNYIYQRNMEKSFNFMAIINEDKWNSFNNTDKLLA
                     IQDSKLAISDIKIKNPNNPARLRNAKLITYHL"
     gene            complement(21143..21547)
                     /locus_tag="ACFJAS_000500"
     CDS             complement(21143..21547)
                     /locus_tag="ACFJAS_000500"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC3981832.1"
                     /translation="MNIIDAIINLANNPVVGVNSHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYNCRRR"
     gene            complement(21656..22783)
                     /locus_tag="ACFJAS_000501"
     CDS             complement(21656..22783)
                     /locus_tag="ACFJAS_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC3981833.1"
                     /translation="MNSSQRKRLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTIGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(22853..22928)
                     /locus_tag="ACFJAS_000502"
     tRNA            complement(22853..22928)
                     /locus_tag="ACFJAS_000502"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22893..22895),aa:Thr,seq:cgt)
     gene            23035..23514
                     /locus_tag="ACFJAS_000503"
     CDS             23035..23514
                     /locus_tag="ACFJAS_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981834.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SDGGFGRRGGWDGTVWFVFGVLAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            23609..24232
                     /gene="rsmG"
                     /locus_tag="ACFJAS_000504"
     CDS             23609..24232
                     /gene="rsmG"
                     /locus_tag="ACFJAS_000504"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC3981835.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            24332..25105
                     /locus_tag="ACFJAS_000505"
     CDS             24332..25105
                     /locus_tag="ACFJAS_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC3981836.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYGVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(25851..26435)
                     /gene="rnhB"
                     /locus_tag="ACFJAS_000506"
     CDS             complement(25851..26435)
                     /gene="rnhB"
                     /locus_tag="ACFJAS_000506"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC3981837.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAKMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(26503..28398)
                     /gene="mnmG"
                     /locus_tag="ACFJAS_000507"
     CDS             complement(26503..28398)
                     /gene="mnmG"
                     /locus_tag="ACFJAS_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC3981838.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRSQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(28571..29998)
                     /locus_tag="ACFJAS_000508"
     CDS             complement(28571..29998)
                     /locus_tag="ACFJAS_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3981839.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            30157..31149
                     /gene="pdxA"
                     /locus_tag="ACFJAS_000509"
     CDS             30157..31149
                     /gene="pdxA"
                     /locus_tag="ACFJAS_000509"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC3981840.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(31379..>32471)
                     /locus_tag="ACFJAS_000510"
     CDS             complement(31379..>32471)
                     /locus_tag="ACFJAS_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFC3981841.1"
                     /translation="RIFGGSETQAVPAAETSEKRSTANRSGSRANNRRRNPRRSKREG
                     SKIEVREAAGKTAGQKARADKAETRNNGNRRRNERGDRATERANEAEIQSRNVQPAAP
                     VADAAPPETEGQTGKRRRNGSRNERGQTAPETAAVAETAVQTAENTPPEPYTAEDKGS
                     KPKSERNRRERDSRDAKERRERNNQRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRS
                     AAAHVFGETDANAPITVSIADPLIATPVQTASSAVSNGDALIYDAAEKIRRAAADILP
                     EGAAPKAAAQEMPSETATFTAAAEQARETAQTGGLVLIETDPAALKAWAAQPEVQAGR
                     GLRRSEQPKPSEAATVPAEEMIQVETRQG"
BASE COUNT         6476 a         8294 c         9635 g         8066 t
ORIGIN      
        1 cgccccggct caaagggaac gattccctaa ggcgcccaag caccgggcga accggttccg
       61 taccatttgt actgcctgcg gcccgccgcc ttgtcctgat ttttgttaat ccactatagt
      121 gtcaacattg aaaaagattg cctgacagtt tgtccgattt caaaatctcc gcgacaagaa
      181 tgttttaaag ccattcgggg atttgggggc ggatgatgcc gtatgccccg ggatagtcga
      241 cgccggtcag gtaaagcccg tcgggcataa aggtcggtgg ggctttgagg cggctgcgtt
      301 cttgaatcag tgcggcgaag ccttcgacgc tgagcctgcc gctgccgaca taaacgagcg
      361 cgcccatgat gttgcgtacc atgtggtgca aaaaggcgtt gccgtgcaaa tcgaggcgca
      421 cgagtcccgc gctttgggta aggccggcgc ggtagatggt tttgacgggg gattttgcct
      481 ggcacccggc ggcgcggaag ctggagaagt cttgttcgcc gatcaataag gcggcagccc
      541 ggcgcatcgg ttcgatgtcg agtttgaggt gtgtccagcc cgccctgttt ttgagcaggg
      601 gggaacggac gggggcggat tcgagcaggt agcggtagtg ccgtccgtat gcgtcgaagc
      661 gggcgtgaaa tccgggtgcg gtctgccggg cgtgcaaaac ggcaatgcct tcgggcaggt
      721 gggcatttac gccgcgcacc catgcctgtt ggggacgggc ggcagttgtg tcaaagtgga
      781 cgacttgggc ggcagcgtgc acgccggtgt cggtcctgcc ggcaactgtg gtggcgacag
      841 attctcctgc gattcgggcg agtgcggttt ccaatgccgc ctgaacggtc ggtacgccgc
      901 ccgcctgttt ctgccagccg taaaagcggc tgccgtcgta ggatagggtt attgcccagc
      961 gttgtttttg tgcggtatcc atcgggtttg ggattcggat aaatgttcag actgcattgt
     1021 atcgcagatt ttgcagggaa acggcaaacg cccggggcgc gcagcgttgt ttggggagtt
     1081 gttcgggggt tggatgcagt tgctacgaat cgctatcctg tgaattcacc ctgtcaggag
     1141 tgcccgaacc gtcattcccg cgcaggcggg aatctaggac gtaaaaatct aaagaaacca
     1201 ttttatccga taagtttccg caccgacagg tctggattcc cgcttatgcg ggaatgacgg
     1261 cggcgggaat cggcggattc ggcggagttg gtggatttgg cgggctgaag cccgcccttg
     1321 tatatcggaa cttccgtatc atagcgacaa accgcccggc cgccacccgc gcccgcccaa
     1381 ggcagacaac cgttgcgcgg ctcagggagc ggcagggcaa cccatcggca caaccgggca
     1441 gttgccaact gtatatggct gccgcggcag cgacacgttt tgaaccgctt attcgggatt
     1501 tccaccaacg cctgctgtat agcggattaa caaaaaccgg tacggcgttg ccccgccccg
     1561 gctcaaaggg aacggttccc taaggtgatg aagcaccagg cggatcggtt ccgtaccatt
     1621 tgtaccgtct gcggcccgcc gccttgtccc gatttttgtt aatccgccat acaagggtaa
     1681 gccgtataag gttgccgtta cggcatgtat gcgcaaactg ccgacgatat tgaatgcccg
     1741 gatgcgtgat tattttgccg caaacgatac cgccgaaaac ggtattcgga cggcttgatt
     1801 tgagttttgg tatttttgcc cgacggggtg gaaaatacag ttgcttttca ctccactttc
     1861 gtacatgcct cacgggaagt tccgtcttcg gacactaatt tgcctgattt atccagttga
     1921 tacacatcat cttttccggg tttgtccatc tgcgcgacca atccggtgta catcatgctt
     1981 tccaagagtc gtccatcctc cgacttttct aaccacgcca gcgtttcggg gctgtgctgt
     2041 cgtgaaactt caccgtactt gatttggctt tgagttaagg cattgttcgc aaaacgccat
     2101 ttacctgcac cgacaacacg gtatgccaag atattgccgt catcatcgat tttgagtata
     2161 gcatcgccac ggaagctgcc gtcctgaaga tatttgactt ttgtatcact gtgaatgttt
     2221 tcatcagtgc cgatgcaatg ccacgtaccg acaatcggag aagccaaggc ggttgttgcg
     2281 gataaaagca ccggcagaat aaagcgtttc atctatttcc ttttttaaag gaggccgtct
     2341 gaaaaccccg gtttcaggtt ttcagacggc cttgtttatt caatcaaatc agtctttcaa
     2401 cttcgccaac tgattttgca cttttgccat tttgtcttcc aattccgcca aatcggcttt
     2461 gtctttttcc accagatgcg ccggggcttt ttcggtgtag ccgggtttgg agagtttggc
     2521 attgagtttg tccaaggctt tttgcagctt ctcggcttct ttgctcaaac gggcggtttc
     2581 ggcggctttg tcgatttcga ctttcagcat caggcgcgcg ccgttgcaga cggcgacggg
     2641 cgcgtctccg ctttcgggca gggcggcgac ttgccgtgct tcggtcaggc gggtcatcat
     2701 cggcaggtat ttgaggtagt ccgccaagtc gtccgcgctt tcgacaaaca gcggggcttt
     2761 gacgttgggc tggatgcccg tttcgccgcg caggttgcgg actgcgccga tcaaatcctg
     2821 caacacggtc atttgcccga atgccgtctg aacaatctcg ccgccgtcgg tttcggggaa
     2881 gcgggcgagc atgatgctgt cggcggtttt ggcatcgcac atgggggcga cggtttgcca
     2941 cagttcttcg gtgatgaacg ggataatcgg gtgcagcagg cgcagggcgg cttcgagtac
     3001 gcgcaataag gtatggcgtg tggcgcgctg acggctggcg cagccggttt ggagctgcac
     3061 tttggcgagt tccagatacc agtcgcaata atcgttccat acgaagctgt acagggtttc
     3121 cgccgccaaa tcaaagcggt aggtttcgta ggcttgcgta acctgctcga tggtctgatt
     3181 cagacgacct atgatccaca tatcggggaa ggaatggccg cgcggttcgg cagcggttgc
     3241 gccgtagccg cagtcttggt tttcggtgtt catcaagacg aagttggtgg cgttccagat
     3301 tttgttgcag aagttgcggt agccttcggc gcgtttgaag tcgaagttaa ccgaacggcc
     3361 caagctggcg tagctcgcca tggtgaagcg caatgcgtcc gcgcccatgc tggggatacc
     3421 ttcggggaag agttttttcg tggcttcttc cactttcggc gcggtttcgg gtttgcgcag
     3481 gccggtcgta cgtttcatca gcagcttgtc caagccgata ccatcgatca aatccacagg
     3541 gtcgatgacg ttgccttcgg atttggacat ttttttgcct tcgtggtcgc gcacgatgcc
     3601 gtggatgtac acggctttaa acggtacttt gccggtgaag tgggtggtca tcataatcat
     3661 gcgcgccacc cagaagaaga tgatttcata tccggtaacc aagacgttgg acggcaggaa
     3721 ggctttgagc tcgtcggttt cagacggcca gccgagcgtg gagaacggca cgagcgcgga
     3781 ggagaaccat gtgtccaata cgtcttcttc gcgggtcaaa cctgttttgc cggcttgttt
     3841 ttcggcttca gcctgattgc gggcgacgta aacattacct gcttcgtcgt accacgcggg
     3901 gatttgatgc ccccaccaca gttggcgcga aatacaccag tcttggatgt tgttcatcca
     3961 ttggttgtag gtattgaccc agttttcagg gataaagcgc accgcgccgc tgtcgacggc
     4021 ttttttggct ttgtcggcaa ggctcaagcc tttgaattcg ttgtcaggct cgccgccgtt
     4081 cggtgtggcg gacatggcga caaaccattg gctggtcagc atcggctcaa tcactgaacc
     4141 tgtgcggtcg cctttcggcg tcatcagcgt gtgcggtttg atttcgacca agaagccttg
     4201 ctcctgcaaa tcggcaacca tttgtttgcg cgcggcaaag cggtccaagc ctgcgtattt
     4261 ttcaggcagg gaaaagcctg gttgcgcttc gcctttgaag ttgaacactt cggcgtttgc
     4321 cagcactttg gcttctaagt cgaacacgtt aatcaggcgc gtgtcgtggc gtttgccgac
     4381 ttcgtaatcg ttgaaatcgt gcgcaggcgt gattttcacg caaccggtac cgaagtcttt
     4441 ttcaacgtat tcgtcggcga taaccggaat ggtgcgaccg gtcagcggca ggattaattc
     4501 tttgccgatt aagtgggtgt aacgttcatc ttctggattg acggcaacgg ccgcgtcgcc
     4561 cagcaacgtt tcaggacggg tggtcgccac gataacggct tcggtgggat tgtcggccag
     4621 cggatagcgg atgtgccaca tagagccttg ttcttccatg ctttccactt ccaaatccga
     4681 caccgccgtg ccaagcacgg gatcccagtt caccaagcgt ttgccgcggt aaatcaagcc
     4741 ctgttcatac aggcgcacga acacttcggc cacggtttcg gcgcgtacgc cgtccatcgt
     4801 gaaatactcg cgcgtccagt cggcggagca gcccacgcgg cgcatttgct gggtaatcgt
     4861 gccgccggaa acttctttcc actcccacac tttttccaag aatttttcgc ggcccaagtc
     4921 atgacgggac acgttttgcg cggcaagctg acgctcgacc acgatttgcg tggcgatgcc
     4981 cgcgtggtcg gtaccgggaa tccaggcggt gttgcagcct ttcatgcggt agtagcgggt
     5041 caggccgtcc atgatggttt ggttgaaggc atgacccatg tgcagcgtgc cggttacgtt
     5101 gggcggcggc agttggatgg aaaaagacgg tttggtcaaa tccatatcgg gccggaaata
     5161 gccttggctt tcccagtttt gatagtgttt ggattcgatt tcggcggggg agtatttgtc
     5221 taacatatga agccctgaga aataagatga tttgatggtt tgaattatag cgcaaatgcc
     5281 gtctgaaacg ctttcggggt ttcagacggc cttaaattta aatattgcct ttacagtttt
     5341 tgatagcgcg acaggctcaa atcgtcgctg cggatttcgg tgtctttgcc gctcacgata
     5401 tcggcggtca attttgccga acccggcgac atggtccagc ccaaagtacc gtgcccggta
     5461 ttcagaaaca ggttttcaaa gcgggtgcgc ccgattaacg gcgtactgtc gggcgtcatc
     5521 ggcctgaggc cgctccagga caatgcttgg ctcaaatcgc cgccttccgg gaacaagtcg
     5581 ttgacgacca aagccaaggt ttcgcggcgt ttttcgggca gtttggtttc gtagccggac
     5641 aattccgcca ttccgccgac gcggattctg ttgtcaaaac gcgtgatggc gactttgtag
     5701 ctttcatcta aaacagtgga caccggtgcg ccgtcggaat ttgtgaccgg cagggtcaag
     5761 gaatagcctt tgacgggata aatgggcaga ttgagatcca actgcgccaa cacagtcctg
     5821 ctgaagcagc cgagcgcgca gacaacggca tctgtttcaa accgccctgt ttcggtttca
     5881 acggctttga tgcgcagccc gttgtggtcg atgcggctga tggtttggtt gaagtagaac
     5941 cgtacgccct tctcttgaca caatttgtac aggttttcgg taaagaggcg gcagtcgccg
     6001 gtcgcatccg caggcaggtg cagaccgccg acaattttgg cggtaacgcg tgccagcgca
     6061 ggctcgaatt ctgcgcattc ttcgggcttc agacggcggt acggcacgcc gtagcgttcc
     6121 aaaacggcaa tgtcttgttt tgccgcttcg acttcttcgg tttggcggaa aatctgcaac
     6181 gtcccttttt tgcgtccctc aaaattcatg tcggtttgcg cttcaaaacg gcggaacatt
     6241 tcacggctgt attcggaaat cctgaccatg cgctctttat tgatttgata gcgcgttgcc
     6301 gtgcagtttt gcagcatccg ccacagccat tcgatttgat acaggccgcc gtcagggcgg
     6361 aacagtaaag gcggatggct tttaaacagc cgtttcagtg ctttggtcgg aataccgggt
     6421 gcagcccaag gcgtggtata gccgtaagaa agctggcctg cattggcaaa actggtttcc
     6481 atcgccacac cctcggtgcg gtcgatgacc gttacttcat gtccggcctc tgccagatac
     6541 cacacggagg atacgccggc aacacccgca cctaaaacaa gcactttcat gtttccccct
     6601 ccggcttttt caaaacaggc ttaatatgcc gtgccgtctg aatattcgga ttcagacggt
     6661 ctcggatatt aatgcggcaa ttcgccgttt gtgatttttt gtttgaagtc gcgtgtttca
     6721 ttgacgatga ctttcgccat caacaaaagc gcagtcaggt tgggcaatgc catcaagccg
     6781 ttgaatgtat ccgaagccag ccataccaaa tcaaggctca acacggtacc cggcatgacg
     6841 gaagaaacat aaccgacgcg gtacaaagcg gcaaacttct cgccgaagac gtaaaccgcg
     6901 catttctcgc cgtaataaca ccagccgaga atggttgagt aggcaaagaa aatcaggccg
     6961 atggtcacaa tccagccgcc gatgccgggc agcatttttt ggaaagtgac ggttgtcagc
     7021 gccgcgccgc tcaattcagg tttcacaaac tcgccgcctg cgccgagcag acccatgacc
     7081 aacacgatac cggtaatcga gcagacaaca atggtgtcca aaaaagtacc ggtcatggaa
     7141 accaaagcct gacgaacggg gtggtcggtt ttcgcggctg cggcggcaat aggcgcagaa
     7201 cccatacccg cctcattgga gaacacgccg cgcgccacgc cgtagcggat gaccgtaccg
     7261 atagcgccgc ctgctacggc ctgcgcgctg aacgcatcgg agaaaatcag cttgacggca
     7321 ggcatcagtg catcggaatt aatgacgata atggaaagac cgcccaacac ataaaacacc
     7381 gccatagcag gcacgatgaa agaagcggct ttggcgatgc ctttaatacc acctaaaacg
     7441 acaacggcag tcagaacggt caacgtaatg ccggtatagg caggttcgat accgaagctg
     7501 gtttgcacag cctgtgcaac cgagttggac tgcaccgagc tgccgatacc gaaggaagcg
     7561 aatgtgccga acagcgcaaa tgcaacggcc atccatttcc agtttttgcc caagcctttt
     7621 tcgatgtaat acatcgggcc gccggacatt tcgcctttgg aattgttgac gcggtatttc
     7681 accgccaaca cgccttcgcc gtatttggtg gccatgccga aaatagcggt catccacatc
     7741 cagaataccg cgcccgggcc gccggttacc actgcagtcg ccacgccggc gatgttaccc
     7801 gtaccgatgg tggcggacag cgcggtcatc aacgccgcaa aatgggaaat atcgccttcg
     7861 tggcctgcgc cgccttcata cttctttgac ggcacaaacg cctgtttcag cgcatagccc
     7921 aacatagtga actgcaagcc tttcagcata acggtcagca caatgccggt gccgaccagc
     7981 agcatcaaca taacagatcc ccaaacccag ctgctgacgg tttcgaaaaa ggctttgaga
     8041 ttgtctaaaa atacttgcat ggctttctcc tttgtctgtt ttatttttaa aacaccactt
     8101 ttgtagtgtc cggtaatttc agcacagaat attcaataag acaatatgtt cttttgaaaa
     8161 atacttttgg ttttttcacc gaagacaggc cggttcaagc tgcggaaatt gtttgcaatt
     8221 atttaaaagc agaggcggag gtcacaatga aatgtccgaa tggggatgtg gcgggcggca
     8281 gaaatcatca atgctgccga ctgccatact tctgaaatct atagtggttt aaatttaaac
     8341 cggtacagcg ttgcctcgcc ttgccgtact tcttgtactg tctgcggctt cgtcgccttg
     8401 tcctgattta aatttaaacc actatacaaa atgatgcatc gatcaaacaa cataccgctt
     8461 taaaaaaaac cgatgccgtc tgaaacgctt tcggggtttc agacggcatt gaaagggtgc
     8521 ggtcagcgga tgatgccgcg cgtcgattgt gcgaagaagt cttgaaatac ggcaagctcg
     8581 gcttgggttt cggcgcggcg gagaatgtcc gccttggctt cttcaaacgg aatgccgcga
     8641 tggtagaggg ttttgtatac gtctttgacg gcggaaatct gttctgcggt aaaaccgttg
     8701 cggcgcatac cttcgctgtt taggccggcc ggttcggcgc ggtagcccga tgccataaag
     8761 tagggcggca cgtctttgtg tacgcctgcg gcgaaggcgg tcatggcgta gtcgccgatg
     8821 cggcagaatt ggaagaccag cgtgtagccg cccaaaacga cgtagtcgcc gacggtaacg
     8881 tgtccggcaa gcgaggcgtt gttggcgaaa atggtgtggt tgccgaccac gcagtcgtgc
     8941 gcgaggtggc agtacgccat aatccagttg tcgtcgccga tacgggtttc gccgatgccg
     9001 gttaccgtgc ctaaattaaa ggtggtgaat tcgcggatgg tgttgccgtt gccgataatc
     9061 agcttggtcg gctcgtcgcg gtattttttg tcctgcggaa tttcgccgag gctggcaaat
     9121 tggaaaatgc ggttgttttc gccgatggtg gtgtggccgt tgatgacggc gtgcggaccg
     9181 atttctgtat tcgcaccgat tcggacgttg gggccgatga cggtgtacgc gccgactttg
     9241 acgccggagt cgagttcggc tttggggtcg atgacggcgg tcgggtggat gagggtcatg
     9301 ttttgaatac tccgctgctt gttaagacaa ataatagaaa atcacagttt gtcgatttca
     9361 tcgcagaccg caatcacttg atcgctaaaa tggctaaata ctgaagcagt atctacatcc
     9421 atgtaattaa gcagttcaaa aatagattgg taaatatttc tagcgtagcc ggtatctttt
     9481 attgcacctt taaaactaaa acaaatcggt tcgtgatgtg ccaaacgatt acggaaattg
     9541 ttaatattgg aaagttctcg aaaaatccat ttttggttat attgcacact tggtgtagat
     9601 ttgggctttt ttggaaaaac tttcatcaat acttttcctg cagcatcaaa ttgagcatcc
     9661 ttccctcccg caaacagata ccgccaaaaa ccgaatccta gtcctgcaac caattggttg
     9721 tggtcaaact ttcctccgct attattttgt attttagata atgcaacctt gattaattca
     9781 gcagattctt tcgtaccatt tcttaaaaag caaccttgat atttcaatgc aggattcgtt
     9841 tgtggttgta tgctatcata taaccggttt ctgtctttaa actcctgccg aaagcaaata
     9901 tcaattttgt ttcttaaaac gatctcaaat atagaaacaa catgaaaaag ttctcttgat
     9961 aaagataaat tcaataaata caattcagct gctttgtctt tcctaacggc atttttatac
    10021 cgttccattc tgaaatcaga aaaaatacta cagcattcct tataattcac gctaaccctt
    10081 gattctttat ttttaaattg acgtttcaaa ctatatttga tatagttaga taaaactaat
    10141 ccccaccgtg tccctgggtt tgaataaacc taaaccggtg ggttttcttt ttattaggtc
    10201 gtctgaaact ttcagacgac ctttttccga acgctcaaac cacgcgtttg gcgcacataa
    10261 tcacggcttc gacggcgact tgtccgtcca ctttggcaac ggcgttgaat ttgccgatgc
    10321 cgcgccggct ggtcaggagt tcgacttcaa agacgagttg gtcgccgggg atgacttggc
    10381 gtttgaaacg ggcttcgtct atgccggcga agaagaaaaa ttcgttttcc ttgcgcccgc
    10441 cttcgctcaa aatcgccaac gtgccgcacg cctgcgccat cgcttcgatg atgagtacgc
    10501 cgggcataac gggcaggtcg gggaaatggc cttggaattg gggttcgttt atggttacgt
    10561 ttttgatggc ggtcagggtt ttcatcggct cgaaggcggt gatgcggtcg agctggagaa
    10621 acgggtagcg gtgggggatg agtttttgga tgtctttggc ttcgatgggg agttgtacgt
    10681 ccatgtctgt cgtattcctt gaataaagtc ggtttggatt tattgcgtgt ctttgctgtc
    10741 tgaaagctgc tgttccagcg ttttgaggcg tttgttcatt tcgcttaagc ggtggatgta
    10801 aacggcgttg cgtgcccatt ctttatgctc ggacatcggg aagatgccgg caaggtgttt
    10861 gccgctttcg gtaatgctgt gggtgacgga cgtgccgccg ccgatggtgg ttttgtcggc
    10921 gatttcgatg tgtccgaccg taccgacgcc gccgccgatg atgcagtagc tgcctatggt
    10981 tacgctgcct gagatgccgg ttttggcggc gatgacggtg tgcgaaccga ttttgcagtt
    11041 gtgtccgatt tggacttggt tgtcgatttt ggtgccgttg ccgacggtgg tgtcgctcat
    11101 cgcgccgcgg tcgatgttgg tgttcgagcc gatttctacg tcgtcgccca gcgttaccgc
    11161 gccggtttgc gggattttga accacgaatc gccggcgaag gcgagtccga aaccgtccgc
    11221 gccgatgacc gcgccgctgt ggatttcgac gtgtctgccg agtgtgcagc cgtaataaac
    11281 gacggcgttg ggatgcagga cgacttcgtc gcccagtttg caatcgtgtt ggacgacggc
    11341 gtttgccaag atgcggcagc cttcgccgag cacggtgttc gcgccgatgt aggcgttcgc
    11401 gccgatttca cagcttgcgg gaacggtcgc gcccggttcg acgacggcgg tcggatggat
    11461 gccgccgcgc gctttgacga cgggtgaaaa caggcgcgcg actttggcga aatagagata
    11521 gggatcgtcg gcgacaatca ggttgcgccc ttcaaatccg tctgctgctt tggcggaaac
    11581 gatgatcgcg cccgcgctgc tgtcgtggac ttcggctttg tatttcggat tggcgaggaa
    11641 gctgatgtgt tccgcctgcg cgtctgcgag cgggcgcacg gcggtaacgg aaatgtcctc
    11701 gccgcgccat tcgccgccga gccgcgcggt gatttgggac agggtgcagg tggccggaat
    11761 catggttttc ctgtttggta tgccgtctga aagggttagc gggcgttcat ttctttaatg
    11821 acgctgtcgg taacgtcgta ttgggtgttg acgtaaatca cgtcctgcaa aatgacatcg
    11881 taaccttcct gtttggcgat tttgacgatg acgcggttgg cgttttgctg gagggaggca
    11941 aactcttcgt tgcggcggag gttgtagtct tcttcaaact gcgcctgttt tttgcggaac
    12001 gcttcgacca gcccgcgcca tttttcttcg gcttgcgcct tttttgcgtc cttaagtttg
    12061 ccgccggcga gctgcctttc caaatccaag ccttcgcgtt gcagtttttg caattcgtcc
    12121 tgacgggcgg aaaattcgcc gtccagcgtt ttttggatgt tgcgcgcctg cttggattcg
    12181 aggtagatgc gctcggtgtt gataaagccg attttttgga aggtgtcggc gtgcgcgcct
    12241 gcggtgcagc acaaaccgat cagggtggcg gcaaacgcgc gggtcaaacg gatcatggta
    12301 aaactccttc ggatgttgcc gcgaaatgcc gtctgaaggg cttcagacgg catttgcggg
    12361 attagaacgt cgtgccgagc tggaattgga agcgttggat ttcgtcttcc ggttttttct
    12421 tcagcgggta ggcgtagctg aatttcatcg gacccaaagg cgagagccag gtaaccgcgc
    12481 cgccggcgga atagcgcaat tcgttggtaa aggtggattt atgcgcgttt tccgagtaaa
    12541 ccgatttgtt gttaccgttt tcggcggcgg tataggttct gccgtcccac acgctgcctg
    12601 cgtcggcaaa caggctcagg cggacggtgc gtgcgtcttt cgcaccgggc atcgggaaga
    12661 gcagctcggc ggagacgttg gcttttttgt tgccgccgta gctgattttt tcgccgtatt
    12721 cgtcatacac tttcgggccg agcgtgccgc tttcgtagcc gcgcaccgaa cccaggccgc
    12781 cgccgtagaa gttttcaaag aaggggattt ctttggttct gccgtagccg cccgcaatgc
    12841 cgacttcgcc gccgagcatc agcgtgaagg ttttgcttaa ggggaagaac caggtttggt
    12901 tgtgggtggc ggagtagtat tgcagtttgc tgccgggcag ggcgatttcg gcatttacgc
    12961 cggtcaggta gccgcgcgtc ggccataagg cgctgtcggt cttgttgcgc ccccagccga
    13021 cagtgccttt gtacagcagg cctttgaagc tgccgtctgc gccgtcggtt ttgccgtatt
    13081 gtttgataaa gtcggcatag cgtttgggtg ctttgttgta ggtgttgacg gtcaggtgtt
    13141 ccgccgccag cccgaaattg acgcggtcgt attcggtaac ggggataccc atccttacgc
    13201 cgccgccggc ggtggtggtt ttatattgtt tgacgctggt cgatgctttg cgcgggtcga
    13261 aggcttttcc gtaaatatcg tagcccaggc tgaccccgtc tgccgtgaag tacgggtcgg
    13321 taaacgacag cgagccgttg agcgtggttt tgcttcgcga ggcgcgcagg gcggccgact
    13381 tgcccgtacc gaacaggttg tcctgcgata cgccggcgga catgaccaag ccggtatcct
    13441 gaacccagcc cgcgctcaag tcgagcgagc cggtggagcg ttcggtcagg ctcatgttca
    13501 aatcgacttt gtcgggcgtg ccggcaagcg ggacggcatc aaactgtacg ttgtcgaagt
    13561 agcccaaaag ctcgacgcgc tctttggaac gttgcagctt ggaggtgtcg taaggcgcgg
    13621 attccatttg gcgcaattcg cggcggacga cttcgtcgcg ggttttgttg ttgccggtga
    13681 tgtggatttc gttgacgtag atttttctgc ccggttcgat gtgcaggacg aaatcgacgg
    13741 ttttggttcc ggcgttcggc agcggctgta cgctgatttc gctgtatgcg tagcctgccg
    13801 agcccatgcg gttctgaatc tcacccaaaa cggcggtcat ctgctggcgt tcgtaccatt
    13861 tgccgggctt catggtcagc agtttttcca gttcggcctt ggggacttcg ttggtgtcgc
    13921 cttcaatcga cactttgccc cagcggaaac gtccgccttc gtggacggtg attttgatgg
    13981 tctgcctggt tttgtcttcg ttggtttgga tgtcggtatc gaggatacgg aaatcgaagt
    14041 agccgttgtt ctggtagaag tcggttactt tttccatgtc ttgggcgaat ttctggcggt
    14101 cgaaccggtc gcttcgtgtc agccatgtcc aaatgccgcc ttcggtcagc gacatctgcc
    14161 gcatcagttt gcggtcggaa tagacttggt tgccttcaaa ttcgatgtcg gtgattttgg
    14221 cggatttgcc ctcgtcaatc gtgatgtcga tgtcgacgcg gttgcgggcg agtttggtta
    14281 ctttgggcgt gatttggata ttgagtttgc cgcgcccgag gtattcttct ttcaggccgg
    14341 cgactgcctg gttgagtgtc gcctgattaa agtattgcga ctgcgccagc ccgaacgatt
    14401 cgaggttttt cttgatggcg tcgttttgca gcattttggc gccggtgatg ttgagcgagc
    14461 cgatggtggg gcgttcgata acggtcagca gaagctgccc gtccgcagtt tcgactcgta
    14521 cgtcgtcaaa gaaaccggtg gcgtacaggc ttttgatgat ggcactgccg tgtgtgtcgt
    14581 tgtaggtgtc gccgactttg acgggcaggt agttgaatac ggtgctcggc tcggtacgct
    14641 gcaagccttc gacacggatg tcttggatgg tgaagtcggc aaatgccaaa ggcgatatgc
    14701 ccaacatcat cagtgcggag gcaatctgtt tcagtttcat cgtaagttcc ttgtggtgcg
    14761 gaatgcggtt tcagactgca ttccgaaacg taaaatctaa ccgatcagcc gggtaacgtc
    14821 gttgaagaag gcggccgcca tcatcagcat catcagggcg agcccgaagc gcaaaccgat
    14881 gttttggaca cgttcgccca aaggtttgcc gcgtatccat tcgacagtat aaaacacgag
    14941 gtgcccgccg tccaaaacgg ggacgggcag caggttcagc acgccgaggc tgatgctgac
    15001 caacgctaaa aattccaaat aactttgcaa gccgagttcg gcggactgtc cggcaatgtc
    15061 ggcaatggtc agcggcccgg aaatatggct gacagaggcg ttgccgctga ttagtttgcc
    15121 gaaaaatttg agggttgtcc acgagtggga aacggttttt tcccagccca tgccgaatgc
    15181 gcggacaaca gacggacggt agctgcggcg gatttgcgcg tcccacgccc tgtccggctg
    15241 cggacggagg ccgacgcgcc cgatcagggt gtggtcgggc tgttcgacag tatcggggcg
    15301 gatgtcggcg gtatgggttt gtccggcgcg ttcgtaggtc agggtgattt ttttgcccgg
    15361 gctttggcgg gtcaggtttg cccattcctg ccatgaggcg atgggtttgc cgtcggcggc
    15421 agtcagcctg tcgcccggtt tcaggcctgc tttttcggcg gggctgcctt tttccacgcc
    15481 gccggcaacg gttgtgattt taaagggcat cagtccgatg tagccttggt tttttgcgat
    15541 tttaccggct tccggcgtgc ctgcggcatc gatggtgcgg acggtttgcg cgcccgatgc
    15601 cgtctgaacg ccgacggcga ctttgccggc ttcgaggttg aggacgattt cggtttgcgc
    15661 gctgctccag tcttggacgg aaacgccgtt gacggattgt attttgtcgc cgctttggaa
    15721 gccggtgcgg gcggcaatgg tgtcgggttc gactgtgccg acatagggcc gcagttcggt
    15781 tacgccgaag gaaaagctca gtccgtacag caaaaccgcc agtgcgaggt tggtcagcgg
    15841 accggcggcg acgatggcga tgcgcttggc ggggtgttgt ttgtcaaaag cgtagggtaa
    15901 atcggcttct gatacttcgc cttcgcgcgt atcgaccatt ttgacgtagc cgcccaacgg
    15961 aatcggggcg aggcaccatt cggtgtcgcc gcgctttcgg gtgaaaaacg gtttgccgaa
    16021 gccgacggaa aaacgcacaa ccttgacgcc gcacaacctg gcgacgatgt agtgtccgaa
    16081 ttcgtgcagg ctgaccaaaa tcaggatggc gaagataaaa gctagaaggg tttgcaaatg
    16141 gttttccttt gataacggtg ttcggacggt atcagcgcag tgtgccgata aatgcccgcg
    16201 cttgtgcgcg tgtccgggca tcttgcgcca acagtccttc tatatcgccc atgccgtttg
    16261 aaaagtcttg tgcaagacag tgggcgacgg ttttggcaat gtcggtaaac ttaatctgtc
    16321 cgtccaaaaa ggcggcgacg gcggtttcgt tggcggcgtt caatacgcag ggcgcggctc
    16381 cgcctgcgtt tatggtttca taggcgaact tcaggcaggg gaagcggccg aagtcgggct
    16441 tttggaaggt cagcgcggac aatgcgccga aatcgagttt gccgacaccc gaatcgatgc
    16501 gctcgggcaa gcccaaacaa taggcgatgg gcgttcgcat atcgggattg cccagttgcg
    16561 ccagcacgga gccgtcgcgg tagcgcacca tactgtgtat cacggattgg ggatggatga
    16621 cgacttcgag tttgtcgggc ggacagttga acagccaatg cgcttcaatc agttccaagc
    16681 ccttgtttgc catagtggct gaatcgacgg agattttgcg ccccatacgc caattggggt
    16741 gtttgaccgc ctgctcgggc gtaatgctgt cgaacgtgct taaatcggtt gttaaaaacg
    16801 ggccgccgga agcggtcagg ataatcgaat cgatgccgtg ttcgttcaga cggtctgtgt
    16861 aatcgcgcgg caaaacttgg aaaatggcgt tgtgttcgct gtcgacgggc aacactgccg
    16921 cgccgtttgc gcgggcggtt tccataaaca acgcgccgga aaccaccagc gtttctttgt
    16981 ttgccagata aatggttttg cctttttgcg ccgctgcgag cgcggaaggc agccccgccg
    17041 ccccgacgat ggcgcacatg acgccgctga cttcgtcggc agacgcaacg tcaaccaatg
    17101 cctgcgcgcc gtgtaaaacc tgagtcgccg tgccgtcgcg tttcaacagg gcttcaagcc
    17161 gggcggcgtg ttcggcatcg gcaacgacgg catattcggg gcggaacgtt tgacattgag
    17221 ccgccagttt ctcgacctgc ttatgccctg ccagcgcgaa tacgcggaat ttttcgggat
    17281 ggcgggagac aacgtccagc gtgctttcgc ctatgctgcc ggtactgcct aatatggtca
    17341 ggacttgtgg tgtcataatg aggataactt tataccggat gccgtctgaa gcgttttcag
    17401 acggcataga atcaatttaa aaccgacatt atcgccgcat agacgctgat aacggcaatc
    17461 aggctgtcgg tacggtcgaa cacgccgccg tgtccgggca gcaggttgct gctgtctttg
    17521 atgcctgccg cgcgcttgag ccagctttcc aacaggtcgc cgcatacgct gacaacggtc
    17581 aacaccaaac cgattaacac ggtatcgaac cagcctgtat cgaatgccag ccagccggca
    17641 cttcgtacgg cagtcatgta cactgccacg caaaccgcac cgccgattgc gccttcccag
    17701 ctcttgccgg ggctgattgc cggtgcgatt ttgtgtttgc cgagcgcctt gccgctgaaa
    17761 tacgcgcaaa cgtcggcaac ccagaccaaa cccatcacgg cgagcagcgg cagggcatca
    17821 tcgggatgcg ggcgcaggga tacgagcgcg aaccaaaacg gcatgagcaa aagccagccg
    17881 acggcataaa cctgccaacc gccgttgagc ctccatttga atctcaacca caaaggcata
    17941 acggcgagcc aaaatgccaa aacaacatac caaaccaaat taggcagcat ccagccgccc
    18001 gcataggcaa ctacgccgaa aaccaaggtt gcggcgaggt aatggttggt ttcggttttg
    18061 cacaaaccgg ccatacgggc atactcccac aaggcggtca gggcaatcag cccgcaaaat
    18121 gcagcccaca gccattgcgg cgcgtaaaac agcatgccca gcatcagcgg cagcagccac
    18181 atagcggtta ttacccgttg tttcagcata ttcagttcct ttgctgtccg ataggcagtt
    18241 gctcggaagt gcgtccgaac cgccgttcgc gtttttggaa cgaagcgacg gcggcatcta
    18301 aggcggtttc gtcgaaatcg ggccacagga tgtcggtgaa atacagttct gcatacgcca
    18361 tctgccagag caggaaattg ctgatgcgcg tttctccgcc cgtgcggatg aacaaatccg
    18421 gttccggcgc gtcgcccagc atcaagtgtt tggccagcgt gtcttccgta atctcggata
    18481 cgccttcggc aatcagtttg tttgccgcct gcaaaatatc ccagcggccg ccgtaatccg
    18541 cagcaatgct cagggtcaga ccggtattat ttgccgtcaa ggcttccgcc tcttcgatac
    18601 cttgcagaat ctgccgatta aagcgttcgc ggctgcccaa tattttcagg cgcatattgt
    18661 tttcgtgcag gcggcgtacc tgtttttgca aagcctgtaa aaacagcccc atcaggaacg
    18721 aaacttcgtc ttcggggcga cgccagtttt cggttgaaaa ggcaaacacg gtcagatatt
    18781 gcacgcccag tttggcgcaa tgcttcacca tattttccaa cgcgtccaag ccgcgtttgt
    18841 gtcccattat acgcgggaga aaacgttttt tcgcccaacg gccgttgccg tccataatta
    18901 cggcgatgtg cctcgggatg gcggtgtgtt ccaaaatggt ctgcgtgctg ctcttcatat
    18961 ctgcctttcg cggttcggcg ttcaaatgcc gtctgaacgc cgcgccgtga gatttaaacc
    19021 gccatcaggt cttcttcttt ggcagccaaa accttatcgg cttcggcaat gtacttgtcg
    19081 gtcagttttt gaaccgcctc ttcgccgcga cgtgcctcgt cttcggaaat ttctttgtct
    19141 ttgaggagtt ttttgatgtg gtcgttggca tcgcggcgca cgttgcggat agagacgcgt
    19201 ccttcttccg cctcgccgcg cacgacttta atcaggtctt tgcggcgttc ctcggtcagc
    19261 atgggcatcg gcacgcggat caaatcgccg acagctgccg ggttcagtcc caagtttgaa
    19321 tcgcggatgg ctttctcgac tttggccgcc atattgccct caaacggttt cacgccgatg
    19381 gtgcgcgcgt ccaaaagggt tacgttggca acctggctga cggggaccat gctgccccag
    19441 tattcgactt ccacttggtc gagcaggccg gtatgcgcgc ggccggtacg cactttcgcc
    19501 agattttctt tcagtacttc gaccgaacgc tgcatcttgc cttcggctgt tttttgaata
    19561 tcgttgatca tattgttctt tcggtaagat aaggtaggcg ggcagccgtt cgaacgcgct
    19621 ccaagccgtt cagacggcat aaagaccgtt aactgcgaat agtaccgtta ttcgggcatg
    19681 acgacaaggc agatgcctgt aggaagggtg tttcaccttt ctagggtttt tttaattgcc
    19741 gctgcaattt cgtaggcaag gttgacgggg acggcgttgc caatcatttt gtatgcgtcg
    19801 ttgacatttt gatagatgaa tttgaagttg tcgggaaagc cttggattct tgcaacttcg
    19861 cgtaccgtca tcctccgata tagtgtttct ttgccggcag caaaacggta gtcgtttgcc
    19921 ccgtgctttt ccatctttgg ggcttgcggg tgcagttggc actgcctgcc tgaagcttgg
    19981 acggtaaaac cctgttcatc ccacgcttta actcggttcc ggctcataaa aataggggaa
    20041 aaactgccgg taaaatattc gttgttgttg actgcgtcgg ggttggtctt gttttgcggg
    20101 gcggaaggta cggctgtgtc ctgcaaatcc caaataacgt ctttcaatgt aatcttgtct
    20161 ttgtcttcga ccgtcgaacc ttttggaaaa gaaaatttta tttccaagtc tttacggaaa
    20221 ccgatgtaga agaccctttt gcgttcctgt gctacaccgt agtctttggc gttggccata
    20281 gtcaaggtta cgtcgtatcc gcatccgtca aacattttca gcaggttttg tacggctccg
    20341 ttgtggcggt ttgccagcat tccgctgacg ttttccgcta aaaagaattt tggctgtttg
    20401 cttttcaaaa tacggatgta gtcgaaaaac aactgtccgc gcgcatcgtc gatgccgcgc
    20461 aaagctcccg cttcagacca agactggcag ggtgggccgc cgataatccc gtcgatttct
    20521 tcagggaaat cttcttcttt aatcttgcgt atatcgcctt ctatcaaatg ggtctttgga
    20581 tggtttgcct tgaaggttgc ccaaatggtt ttatcgtatt cgttggcggc ggggatttca
    20641 aatcccgctt tttcgaaacc caaatccaaa ccgccgcaac cgctaaacaa actaatgatt
    20701 ttcataggat tataagtggt aggtgatgag ttttgcattt cttaatcgcg ccgggttgtt
    20761 gggattttta attttaatgt cggaaatggc cagtttggaa tcctgtatgg caagcagctt
    20821 gtctgtattg ttaaaactgt tccatttgtc ttcgttgata atggccatga aattgaaact
    20881 tttttccata ttccgttggt aaatatagtt gaagacaaac catggatttt cgattcccca
    20941 cataccccgt actctcagat aagttatgtt gagcggatca atgcggttga ccctgcccag
    21001 ctctttggtt tctgcaaact gtatgccgcc gatgttgccg attccttcct taatttgatt
    21061 ttttatcttc aaatagcatt cggcatcggc gcagtagtcg attccgtaaa ccattgccaa
    21121 gtgtttgagg tttttctttt tgtcaacgac gccgacaatt atagataatg tctttctctt
    21181 cccatttttc cgcatctttg caggcttttg taagcatgct gtcatcaaca gagagctttg
    21241 attttgggtg gctgctattc agcgccagtg cggaatcttt actttcaatt ttcttaactt
    21301 caatggcatc cccgtttcgc aacatggcat caggagggtt ggagttgttg cccaaatatg
    21361 agaatacttt tgcgtgtcgg gctatgcgtt gggtttcatt taggttgaaa ctgccggaaa
    21421 acaagtcttt tacatattct tccaaagcat cacctgcctg attggcacga ttgttgcttt
    21481 gagaatggga gttgaccccg acaacagggt tgttagccaa attgatgatg gcatcgataa
    21541 tgttcatggt ttttccttgg ggttttgcgg cagattatag caaaaccgtc agggtgttta
    21601 tcggaccgat gccgtctgaa gcggcaggcg tttcagacgg catcgtgtcc ggccgtcagc
    21661 cgtgttcccg tgtttcaagc aggctttggc gcaggtgttg gcgttcgtgg gcatccagcc
    21721 atttgcggcg ggtgcgttgc agcaggatga cgagggcgga aatttcctga cgcatattgg
    21781 tgctgagcca gaggaagccc tgccattggt agtggaggtg ttcggcgagg gcttccagtt
    21841 cggggttgat ggcggtgtcg atgcggatgc ggcgggcgtg tctgccgttg atgagggcgg
    21901 cggtttgttg caggtcggtt tggagcagtg tgaagtggcg gtcgagcagc cggatttcgc
    21961 tgccgttgag tttgggagat tgcagcttgg cggcggtggt caggagcagc tcggtggtgt
    22021 tgacgatttt gcggtgggcg tgctgcatgg cttccatcat ggaggggctg atgcggcttt
    22081 cgcccgatgt ggcggcgagg tggctgcggc ttttgaccat gcgtgcgttg atttggcgca
    22141 ttttgaccat attctgctcc aaacgttcgc gcgtcatacg cctgccgttg ctgatttcgg
    22201 caatcatttt gctgcagtcg gccaggttgt cggcaagcat gaaacgccac atcagtgtgg
    22261 atttcagcgg cagcagtttg gcggcggcaa tggcgatggc ggcgccgatg aggacgttca
    22321 tcgcgcgcat caggccgctg tcgagccatt cgctgccgtt gtcgccgatg agcatgcaca
    22381 tcgtcagccc cgccagcata gggacgtagc cgtttttgcc gaccgccgcc cagccggcca
    22441 gtgcgcttgc cgtgccgatg gtcaggtaga agaggaggtt gccgtggaaa taatgctggt
    22501 tcagccataa aacgcccaaa cccgcgccca gcccgatgac cgtaccgagc atacgttcca
    22561 ccgccttgga gtaaatcgcg ccttggaact ggagcatgcc gaggacgacg aagacggtca
    22621 tccctatcca ttcgccgtgt tggaggtgga gtagccgggc gagtgcggtg gcgaacagga
    22681 cggctccgcc gagccgcacg gcatgtatga ggcggcggtg gcggtagcgt tcgtaggagt
    22741 tgagccagcg gccggaaagg cgtttgcgtt gcgaggagtt catctcgtat gccgtcttct
    22801 gcttgaaaaa aaaaaagcac aggcttcccg agggagggtc tgtgcttggt attggtgccg
    22861 gagaagggaa tcgaaccccc gaccttcgcg ttacgaatgc gctgctctac cgactgagct
    22921 acaccggcgt ttttcgttat gatatatata aacggttgtt tgtgcaactt ttcgggcggg
    22981 cggcaaggcg gtgcgcggta taatgcggcc tgcttcggaa gaggggggac ggcgatgttt
    23041 gtgaacgaga aatatcctta tgcgaccctg tttgcggggc tggtgttttt gacgctgccg
    23101 tttgcgttgg cggtgcatga tgcctttgca tgtgcgttcg gacgggcggg gctgctggtg
    23161 tcggtgtcgg acggcggatt cggccggcgt ggcggttggg acggcactgt ttggtttgtg
    23221 ttcggtgtgc ttgcgttttt gaatgtggtt gtgtcggcgg gcctgacgaa actggcgtac
    23281 aaaaagatga tgcggcggca ttcgcgttac gcgctgtttc tgtcgggcgt ggcggcttgc
    23341 gcggcggtgg cttggatttt caagctgttg ttgggcagtg ccgccttggg cgggctgagc
    23401 ggggaggcgg tgtcggaata tgcgtttgcc gtgtggctgg tgtctatgct gacgctgccg
    23461 aaacgcctga cgcgcgcgcc ggtgcagcct gtggtgttcc acaggaaaaa ataggacgga
    23521 acggaaatgc cgtctgaaac ccgatacgcg gtttcagacg gcatgttttt ccgctaacat
    23581 tacgcctgaa tatggacagg aagcagatat ggaacgtaaa gaacgcctgc gtgcaggcat
    23641 tgccgcgatg gggccggata tttcggaaac ggcgcaggac aggcttttgg cctatgtgga
    23701 tttgttgaaa aagtggaaca aaacctacaa tctgaccgcc ctgcgcgacg aggaaaaaat
    23761 gattgtccat catcttttgg acagcctgac gctgctgccc tatatcgaag gcgcacagac
    23821 gatgctggat gtcggttcgg gcggcggcca gcccggcatt ccggcggcgg tgtgccgtcc
    23881 ggatgtgcag ataaccttgt tggatgcgaa tacgaagaaa acggcttttt tgcggcaggc
    23941 ggctatcgag ttggggttgg acaatgtgcg cgtggtgtcg gggcgcgtgg aggcggtttc
    24001 ggacgtgcgt gccgacgtgg ttaccagccg tgcgtttgcg gaactggcgg attttgtgtc
    24061 gtggacggcg cacctgttga aagacggcgg ctactgggcg gcgatgaagg gcgtatatcc
    24121 gcagggggaa atcggccgcc tgccgcagga tgtgtgcgtc gaaaaagtcc aaaggctcga
    24181 cgtgccgggc ttggatgcgg aacgccatat cgtcatcctg agcaagcgtt gagcgcgctt
    24241 cagacggcat gaataccttt tttgtgcgga taaaggtaaa attccgcact gtttttcttt
    24301 ttttcaacat cagacgggac acgggcggga catgagtgcg aacatccttg ccatcgccaa
    24361 tcagaagggc ggtgtgggca aaacgacgac gacggtaaat ttggcggctt cgctggcatc
    24421 gcgcggcaaa cgcgtgctgg tggtcgattt ggatccgcag ggcaatgcga cgacgggcag
    24481 cggcatcgac aaggcgggtt tgcagtccgg cgtttatcag gtcttattgg gcgatgcgga
    24541 cgtgcagtcg gcggcggtac gcagcaaaga gggcggatac ggcgtgttgg gtgcgaaccg
    24601 cgcgctggcc ggtgcggaaa tcgagctggt gcaggaaatc gcccgggaag tgcgtttgaa
    24661 aaacgcgctc aaggcagtgg cggaagatta cgactttatc ctgatcgact gtccgccttc
    24721 gttgacgctg ttgacgctta acggcttggt ggcggcgggc ggcgtgattg tgccgatgtt
    24781 gtgcgaatat tacgcgctgg aagggatttc cgatttgatt gcgaccgtgc gcaaaatccg
    24841 tcaggcggtc aatcccgatt tggacatcac gggcatcgtg cgtacgatgt acgacagccg
    24901 cagcaggctg gttgccgaag tcagcgaaca gttgcgcagc catttcgggg atttgctttt
    24961 tgaaaccgtc atcccgcgca atatccgcct tgcggaagcg ccgagccacg gtatgccggt
    25021 gatggcttac gacgcgcagg caaagggtgc caaggcgtat cttgccttgg cggacgaact
    25081 ggcggcgagg gtgtcgggga aataggtcaa tccaaatcgg gctgcccgtg cctttatgct
    25141 gtttggccgg gtgcgttaat atggcggatt aaaataaaaa tacttaatat cgtcatttat
    25201 agcggattaa caaaaaccgg tacggcgttg ccccgcctta gctcaaagag aacgattccc
    25261 taaggtgctg aagcaccaag cgaaccggtt ccgtactatc cgtactgtct gcggcccgct
    25321 gccttgtcct gatttttgtt aatccgctat atcgtcattc ccgcaaaaac aaaaaaaatc
    25381 aaaaacagaa aactgaaata tcgtcattcc cgcgcaggcg ggaatctagg tctgtcggta
    25441 cggaaactta tcgggaaaaa cggtttttcc aaccctgaga ctccggattc ctgttttcgc
    25501 gggaatccgg ttttttgagt ttcagtcatt tttgataaat tcttgcagct ttgagtttct
    25561 agattcccgc ttttgcggga atggcggttt ggaagttacc tgaaattcaa aaaaaaacgg
    25621 aaaccggacg gattggattc ccgcctgcgc gggaatgacg gattttaggt ttttttgatt
    25681 ttctgttttt cgcgggaatg acggttggga agtttcccga aacttaaaaa caactgaaac
    25741 ccaaaaaact aaattaccgc ctgcggggga atgacggttt gggttctttc tctttgaagt
    25801 tgcgatgctg gaaatgccgt ctgaagttca gacggcattt ttgtgccggt ttaaaacaag
    25861 gcctgctgcg cgagcaggtt tctgacgggg gcgaagtcgc ggcggtgttc gggcagcacg
    25921 ccgtattgtt tgagggcttc cagatgctgc tttgtgccgt aacctttgtg tttgtcaaaa
    25981 ccgtattggg gacggcgttg cgccagtgcg tacatttccg catcgcgtgc ggtctttgcc
    26041 aaaacggatg cggcggagat ttcgatgatt ttgctgtcgc ctttgacgac tgcttcggca
    26101 gggatgccca aatgttcggg aatgcggttg ccgtcgatga atattttttc gggacgcgca
    26161 gccaaaccgt aaacggcacg tttcatggcg agcatggtgg cgtgcaggat gttgaggctt
    26221 gcgatttctt cgggcgtgga ggcggcgacg tgccacgcga ccgcctgttc tttaatcatt
    26281 ttggcaagcg cgtcgcgttt tttctcgctg agttttttgg agtcggtcag tccgggcagg
    26341 tcgaatgttt ccggaaggat gacggcggcg gcaaacacgc tgccgaccaa aggtccgcgt
    26401 cctgcctcgt ccacgccggc ggtcagtatg tgcatgatgt ttcctgtcgg gatggtacga
    26461 tgccgtctga aaatggtttc agacggcatc gtaccgatgt gtttatttcg cgtctttaaa
    26521 cccgcgcttc aaatgcacca tcagcagtgc gacggcggca ggcgtaacgc cggaaatgcg
    26581 gcttgcctgc ccgacggttt cgggtttgtg ctggttgagc ttttgttgca cttcggccga
    26641 caagcctttg actctgccgt aatcgatgcc gtcgggcagc tttaaggttt cgatgtcgcg
    26701 gcggctgtcg atttcttcgt tttggcggtc gatatagcct tggtatttga cttggatttc
    26761 gacttgctcg atgacttcgg cagagaggtt ttcagacggc atcgcgcctt cgagcgtcat
    26821 cagcgcggcg taatcgaggt tcgggcggcg taacaaatcg tgcaggttgg cttcgcggct
    26881 gagtttttgt ccgaacacgc ggatttgttc gtcttcggcg agtttttgcg gcgtgtacca
    26941 cgttgttttc aaacgttgga tttcgcgttc gacggcttcg cgtttttcgt tgaacatgcg
    27001 ccattgcgct tcacccacca agccgatttt gtagccgtct tcggtcaggc gcatatcggc
    27061 gttgtcttcc ctgagttgca ggcggtattc ggcgcggctg gtaaacatcc ggtaaggttc
    27121 gttcaggcct ttggtgatga ggtcgtccac caatacgccg aggtaggctt gttcgcggcg
    27181 cagcaggagc ggatcttgtc cgcggacgta ttgcacggcg ttcgcgcccg ccagcaatcc
    27241 ttgcgcggcg gcttcttcat agcctgtcgt gccgttgatt tgcccggcga aaaacagtcc
    27301 ctcaatggtt ttggtttcga ggcttgcttt gaggttgcgc ggatcgaagt agtcgtattc
    27361 gatggcgtag ccggggcgca ggatatgggc gttttcaaga cccttcatac tgcgcacgag
    27421 cgcgatttgg atgtcgaacg gcaggctggt ggagataccg ttggggtagt attcgtgcgt
    27481 ggtcagacct tcgggttcga ggaaaatctg gtggctgtct ttgtcggcga agcggttgat
    27541 tttgtcttcg atagacggac aataacgcgg acccacgcct tcgattttgc cggtaaacat
    27601 cgggctgcgg tcgaagcctg agcggatgat gtcgtgggtt tgcgtgttgg tatgcgtaat
    27661 ccagcaggac acttggcgcg ggtgcatttc ggcgttgccg cgcacggaca tgacgggaac
    27721 gggcgtgtcg ccgggctgtt cggtaagttg ggaaaagtca atcgtgcgtc cgtcaatacg
    27781 cggcggcgtg ccggttttca gacggccttg cggcagcttc aattcgcgca aacgtccgcc
    27841 gagcgatttg gcggctgggt cgccggcgcg gccgccttcg tagttttcca aaccgatgtg
    27901 gattttgccg gacaagaacg tgcctgcggt cagcaccacg gcgcgtgctt taaactccgc
    27961 gcccatcgcg gtgatcacgc cgctgatgcg ttcgccctcg agtgttacgt cttcgacggc
    28021 ttgttggaaa aggtcgaggt tttcttggtt ttccaacatt tcgcggatgg aggctttata
    28081 caggatgcgg tctgcctgcg agcgcgtggc tcgtactgcc gcgcctttgc tggcgttcag
    28141 gcggcggaat tggatacctg atttgtcggt cgccaacgcc atcgcgccgc ccaatgcgtc
    28201 gagttcgcgt accaaatgcc ctttgccgat gccgccgata gaggggttgc acgacatttg
    28261 tccgagcgtt tcgatattgt gtgtgagcaa aagcgtctgc gcgcccatac gggcggcggc
    28321 aagcgcggct tccgtgccgg cgtgtccgcc gccgacgacg atgacatcgt aggttttggg
    28381 gtaaatcatg tgggtcatgt gtgtattgcc tatcggtttt tcagacggca ttccaagcag
    28441 gatgccgtct gaaaaacgaa acggattcaa aagtaaaggg ttgggattgt acgcctgttc
    28501 gccctgtttt tacagtgcgc ggaaagggaa aagccgcttc gcggggaagc ggctccggta
    28561 agggcgggat ttaccaaaca tcggatttga tgcggcgttt caggcccgga tgttcggaaa
    28621 gtttgaactc ggggtctttg cccattttca gcttggcggt gtaatcgcgc agcagcataa
    28681 acgccaatgg cgagagcagc aggatggcga cgaggttgat ccacgccatg atgcccatcg
    28741 ccatatccgc catatcccag accaaaggca cattggcaac cgcgccgaaa tagacccacg
    28801 ccaaaaccag catacggaaa acggcggtaa tcagccaatg gcttttgatg aattggacgt
    28861 tggactcggc ataggcatag ttgccgataa cggtggaaaa ggcaaacata aacaggatga
    28921 cggcgaggaa acccgcgccc cattgcccca cttggctgac aatcgccgcc tgcgtcagcg
    28981 ccgcaccgct caaatcgcca taaggctgtt ggtaaatcaa gatgatgaag gcggtgcaag
    29041 aacaaacgat gatggtatcg acaaacacgc ccagcatttg aatcatacct tgcgaaacag
    29101 ggtgtttcac ttcggcggcg gcggcggcgt tcggcgcgga acccataccc gcctcgttgg
    29161 aatacaggcc gcgtttgatg cccatcatca tcgtttgcga aatcagaccg ccgagtaagc
    29221 cgcctgctgc cgcgtcgaat ttgaacgcgc ccgaaaaaat ctgaccgaac acgtccggaa
    29281 tcatcggaat attggtcaaa atgatgaaaa gcgcgataaa gaggtacaaa accgccatca
    29341 ggggcacgac gatttccgcc gctttggata tgcgcctgat gccgccgaag ataatcggcg
    29401 cggttaaaat caccagggcg acgccgacat aatgaggctc ccagccccac gccgctttga
    29461 cggtatcggc gatggtattg gtctgaaccg cttcaaacac aaaaccgaaa cagaaaatca
    29521 ggctcagggc gaacaacacg cccagccatt tctgccccag accgtgcgtg atgtagtagg
    29581 cagggccgcc ccggaaatgg tggttgtcgc agtcgcggac tttaaagagc tgcgccagcg
    29641 aagattcgac aaacgccgaa ctcatgccga ttaaggcggt tacccacatc caaaacaccg
    29701 cgcccggccc gccgactttg atggcgattg ccacgcccgc gatattgccc acgcccacgc
    29761 ggctggcaag gccggttaca aatgcctgaa acggcgtgat gccgtgaggg tcgtccccct
    29821 gtttgcggcc gccgagcatt tctttgatgc tgcgcccgaa caggcggaat tggacaaagc
    29881 ccgtggttac ggtgaagaaa agccccgtac ccaaaagcat ataaaccaag tatgaccaca
    29941 tcggatcgtt gatggcgccg acccagccgt gcagccattc ggtaaagttc tcgttcatat
    30001 cgcttcctta aagttgaaac ccgcacatat ggcggtatgc aagcagggtt taaattttgt
    30061 aaacgcccat tctagcagat tgtcaacaaa atcagaaaaa tttacatcgc cgcgcgcctg
    30121 tggcgttaga atcgcgtttt gtttggagca aacacgatga aacagcctgt ttttgccgtt
    30181 acttccggcg agcctgccgg catcggcccc gatatttgtt tggacttggc gtttgcacgc
    30241 ctgccctgcc gctgcgcggt attgggcgac aaacacctgt tgcgcgcgcg cgccgaagcc
    30301 ttgggcaaaa gcgtcgtcct gcgcgacttc gatccggaat caggcggcgc gcacggagaa
    30361 ggcgggctgg aagtgctgca catccctgcc gccgaagcgg ttgaggcagg gtggctcaat
    30421 cccgccaacg ccgcctatgt gctgcaactt ttggacaccg cgctcgcagg catttcagac
    30481 ggcattttcg acggcatcgt taccgcgccg ctgcacaaag gcatcatcaa cgccgcgcgc
    30541 gcaagcacag gttttttcag cggacacacc gaatatctgg cggaaaaaag cggcacgggg
    30601 caggtcgtga tgatgcttgc cggcaaaggc ctgcgcgtcg ccctcgtaac gacccacctg
    30661 ccgctgaaag acgttgccgc cgccatcacg caaccgctga ttgaaagcgt cgcacgcatt
    30721 ttgcatcacg acttaaaaca caaattcggc atcaaaaatc ccaaaatcct agtcgccggg
    30781 ctcaatcccc acgccggcga aggcggacac ctcggacacg aagaaaccga caccattatc
    30841 cccgcgctcg aaaacctgcg ccgcgaaggg ataaacctcg ccggcccgta tccggcggac
    30901 acattgttcc aaccgtttat gctcgaaggt gcggatgccg tattggcgat gtaccacgac
    30961 caagggctgc ccgtgttgaa ataccacagc ttcgggcagg gcgtgaacat cacgctgggg
    31021 ctgcccttta tccgcacctc cgtcgatcac ggcaccgcgc tcgatttggc ggcaaccggc
    31081 agggcggact ccggcagcct gataactgcc gtggagaccg ccgtcgagat ggcgcgcggc
    31141 agcctttaaa gatgataaaa gacccgtcat tcccgcgcag gcgggaatcc ggaccttcgg
    31201 gcggcggcaa tattcaaagg ttatccgaaa gtttgaggtt ctagattccc gttttcacgg
    31261 gaatgacgaa aggtggcggg aatcacggga atggcgaagt ttcagacggt attgcaggta
    31321 tccgaaacca tgcaaaaaga ggttctgcgg aacagaacct ctttttgccg ccgtcggttc
    31381 agccttgccg ggtttcgact tggatcattt cttcggcagg gacggttgcg gcttcagacg
    31441 gttttggctg ttcggaacgg cgcaaaccgc gtccggcttg gacttcgggt tgtgccgccc
    31501 atgccttcaa tgcggcaggg tcggtttcga tcaggacgag tccgccggtt tgtgcggttt
    31561 cccgtgcctg ttccgccgca gccgtaaagg ttgcggtttc agacggcatt tcctgtgctg
    31621 cggctttcgg tgccgcgcct tcgggcagga tgtcggcggc ggcacggcgg attttttccg
    31681 ccgcatcata aatcagtgcg tcgccgtttg aaacggcgga agatgccgtc tgaacgggtg
    31741 tggcgatgag cggatcggca atgctgacgg taatcggcgc gtttgcgtcg gtttcgccga
    31801 aaacgtgcgc ggcggcggaa cggactttgt cggcggtgtc gtgaatattc aggtactgct
    31861 cgattttggc ggcagacggg atattgcgtt ttttgccgtt ttgacggcgg tcgcgctgat
    31921 tgttgcgttc gcggcgttct ttggcatcgc ggctgtcgcg ttcgcggcgg ttgcgttcgg
    31981 atttgggctt gctgcctttg tcttctgcgg tatacggttc gggcggcgtg ttttccgctg
    32041 tctgaacggc tgtttcggcg acggcggcgg tttccggtgc ggtttggcca cgttcgttgc
    32101 ggctgccgtt gcggcggcgt tttccggttt gaccttcggt ttcgggcggt gcggcatctg
    32161 caacgggtgc ggcaggctgt acgttgcggc tttggatttc cgcttcgttg gcgcgttcgg
    32221 tggcacggtc gccgcgttcg ttgcggcggc ggttgccgtt gttgcgcgtt tcggctttgt
    32281 cggcgcgcgc tttctgtccg gcagttttgc ccgccgcttc gcggacttct attttgctgc
    32341 cttcgcgttt gctgcggcgc gggtttcggc ggcggttgtt ggcgcggctg ccgctgcggt
    32401 ttgccgtgct gcgtttttcg gaggtttcgg cagcgggtac ggcttgggtt tcgctgccgc
    32461 cgaaaatgcg t
//
LOCUS       DAXFZR010000013        31494 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000013 DAXFZR010000000
VERSION     DAXFZR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31494
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.013"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            249..1623
                     /locus_tag="ACFJAS_000511"
                     /pseudo
     CDS             249..1623
                     /locus_tag="ACFJAS_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
     gene            complement(1685..2437)
                     /gene="rlmB"
                     /locus_tag="ACFJAS_000512"
     CDS             complement(1685..2437)
                     /gene="rlmB"
                     /locus_tag="ACFJAS_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC3981842.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEGILENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGDADLYHCNLPDSTAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(2503..2991)
                     /locus_tag="ACFJAS_000513"
     CDS             complement(2503..2991)
                     /locus_tag="ACFJAS_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC3981843.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(2996..3715)
                     /gene="tadA"
                     /locus_tag="ACFJAS_000514"
     CDS             complement(2996..3715)
                     /gene="tadA"
                     /locus_tag="ACFJAS_000514"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC3981844.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIITSAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(3719..4660)
                     /gene="miaA"
                     /locus_tag="ACFJAS_000515"
     CDS             complement(3719..4660)
                     /gene="miaA"
                     /locus_tag="ACFJAS_000515"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC3981845.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGRPMSAHLNGQPEHTLPFELYTAALIPEDRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            5011..5289
                     /locus_tag="ACFJAS_000516"
     CDS             5011..5289
                     /locus_tag="ACFJAS_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981846.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            5308..6459
                     /gene="earP"
                     /locus_tag="ACFJAS_000517"
     CDS             5308..6459
                     /gene="earP"
                     /locus_tag="ACFJAS_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC3981847.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHLLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            6502..7062
                     /gene="efp"
                     /locus_tag="ACFJAS_000518"
     CDS             6502..7062
                     /gene="efp"
                     /locus_tag="ACFJAS_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC3981848.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(7242..8039)
                     /locus_tag="ACFJAS_000519"
                     /pseudo
     CDS             complement(7242..8039)
                     /locus_tag="ACFJAS_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
     gene            complement(8156..8737)
                     /gene="metW"
                     /locus_tag="ACFJAS_000520"
     CDS             complement(8156..8737)
                     /gene="metW"
                     /locus_tag="ACFJAS_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC3981849.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            complement(8734..9873)
                     /locus_tag="ACFJAS_000521"
     CDS             complement(8734..9873)
                     /locus_tag="ACFJAS_000521"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC3981850.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSAQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            10016..10234
                     /locus_tag="ACFJAS_000522"
     CDS             10016..10234
                     /locus_tag="ACFJAS_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981851.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKNAV"
     gene            complement(10320..10445)
                     /gene="ykgO"
                     /locus_tag="ACFJAS_000523"
     CDS             complement(10320..10445)
                     /gene="ykgO"
                     /locus_tag="ACFJAS_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC3981852.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(10445..10720)
                     /locus_tag="ACFJAS_000524"
     CDS             complement(10445..10720)
                     /locus_tag="ACFJAS_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC3981853.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            10919..11797
                     /gene="metF"
                     /locus_tag="ACFJAS_000525"
     CDS             10919..11797
                     /gene="metF"
                     /locus_tag="ACFJAS_000525"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC3981854.1"
                     /translation="MNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGDRDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGARP"
     gene            11933..14209
                     /gene="metE"
                     /locus_tag="ACFJAS_000526"
     CDS             11933..14209
                     /gene="metE"
                     /locus_tag="ACFJAS_000526"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC3981855.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSIEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNAGQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            14488..15225
                     /locus_tag="ACFJAS_000527"
     CDS             14488..15225
                     /locus_tag="ACFJAS_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC3981856.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            15474..16877
                     /locus_tag="ACFJAS_000528"
     CDS             15474..16877
                     /locus_tag="ACFJAS_000528"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3981857.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQTDEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            17164..17541
                     /gene="sdhC"
                     /locus_tag="ACFJAS_000529"
     CDS             17164..17541
                     /gene="sdhC"
                     /locus_tag="ACFJAS_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC3981858.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            17535..17876
                     /gene="sdhD"
                     /locus_tag="ACFJAS_000530"
     CDS             17535..17876
                     /gene="sdhD"
                     /locus_tag="ACFJAS_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC3981859.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            17879..19642
                     /gene="sdhA"
                     /locus_tag="ACFJAS_000531"
     CDS             17879..19642
                     /gene="sdhA"
                     /locus_tag="ACFJAS_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC3981860.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            19762..20469
                     /locus_tag="ACFJAS_000532"
     CDS             19762..20469
                     /locus_tag="ACFJAS_000532"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC3981861.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            20473..20721
                     /locus_tag="ACFJAS_000533"
     CDS             20473..20721
                     /locus_tag="ACFJAS_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC3981862.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            20833..22116
                     /gene="gltA"
                     /locus_tag="ACFJAS_000534"
     CDS             20833..22116
                     /gene="gltA"
                     /locus_tag="ACFJAS_000534"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC3981863.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            22321..25149
                     /locus_tag="ACFJAS_000535"
     CDS             22321..25149
                     /locus_tag="ACFJAS_000535"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC3981864.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            25249..26430
                     /gene="odhB"
                     /locus_tag="ACFJAS_000536"
     CDS             25249..26430
                     /gene="odhB"
                     /locus_tag="ACFJAS_000536"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC3981865.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVTALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            26691..28124
                     /gene="lpdA"
                     /locus_tag="ACFJAS_000537"
     CDS             26691..28124
                     /gene="lpdA"
                     /locus_tag="ACFJAS_000537"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3981866.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAVDKRALHG"
     gene            28244..28531
                     /locus_tag="ACFJAS_000538"
     CDS             28244..28531
                     /locus_tag="ACFJAS_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981867.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQIYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            28549..29769
                     /gene="sucC"
                     /locus_tag="ACFJAS_000539"
     CDS             28549..29769
                     /gene="sucC"
                     /locus_tag="ACFJAS_000539"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC3981868.1"
                     /translation="MGLAAGRFLKPTHLKEKSMNLHEYQAKELLASYGLPVQGGILAH
                     NGEEAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVNSREKAKEVAESLIGTNLVTY
                     QTDANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETP
                     EKIFKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVN
                     PLAVRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEG
                     NIGCMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVL
                     INIFGGIVRCDMIAEAIVAAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADG
                     LNDAAEKIVAAVNA"
     gene            29780..30670
                     /gene="sucD"
                     /locus_tag="ACFJAS_000540"
     CDS             29780..30670
                     /gene="sucD"
                     /locus_tag="ACFJAS_000540"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC3981869.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTNLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(31053..31439)
                     /locus_tag="ACFJAS_000541"
                     /pseudo
     CDS             complement(31053..31439)
                     /locus_tag="ACFJAS_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7559 a         7901 c         8316 g         7718 t
ORIGIN      
        1 atttttttta taatcatgaa attattgggt ttaagcagtg gatgcgccgt tgaggattaa
       61 aatgtgttaa gcgcatgaag gctctctgga aatgaaggac tgtccggtgc ggaaattcaa
      121 agaaatttga attcggacaa ccggtggtgt ttttgcacat gaggatttga attgataagg
      181 aatctttgat tcgttacaat ttcgttctta aaatttttta acgtttggca acggatagaa
      241 agactccaat ggctgaaaca atgaaaaaac aggcggattc gcctgatttg gtgtacggtt
      301 tggaagacag gccgccgttc ggtaatgcgc tcttgagcgc ggttacccat cttttggcga
      361 ttttcgtgcc gatgattacg cccgcgctga ttgtgggcgg cgcgctggaa ctaccggtgg
      421 aaatgacggc gtatctcgtg tcgatggcga tggttgcgtc gggtgtcggc acttatttgc
      481 aggtcaaccg cttcgggccg gtcggttcgg ggatgctgtc catccagcgt taccgtcatg
      541 attgcgctcg gcgcggggat gaaagagggc ggtttgagtg agggggcgat ggtttcgacg
      601 ctcttgggcg tatcgtttgc cggtgcgttt ttggtgtgtt tctcggcgtg gcttctgccg
      661 tatttgaaaa aagtgattac gccgacggtc agcggcgtgg ttgtgatgct gattggtctg
      721 agtttggtac acgtcggtat tgccgatttc ggcggcggct tcggcgcgaa ggcggacggc
      781 acgttcggct cgatggaaaa cttggggctg gcttcgctgg tgctgctgat tgtgctggtg
      841 ttcaactgca tgaaaaatcc gctgctgcgg atgagcggca ttgcggtcgg tctgattgcc
      901 ggctacattg tcgcgctgtt tttgggcaag gtggattttt ccgcgctgca aaacccgccg
      961 ccggttacgc tgcccgtacc gtttaaatat ggttttgcgt tcgactggca cgcatttatt
     1021 gcggcgggtg cgattttctt gttgggcgtg tttgaggcgg tcggcgattt gacggcaacg
     1081 gcaatggtgt ccgaccagcc gattgaaggc gaagaataca ccaaacgcct gcgcggcggc
     1141 gtgttggctg acggcttggt atcggtgatt gcgacggctt tgggttcgct gccgctgacg
     1201 acgtttgcgc aaaacaacgg cgtgattcag atgaccggag tggcttcgcg ccacgtgggc
     1261 aaatatattg ccgtgatttt ggtgctgttg ggtctgttcc ccgttgtcgg acgcgcgttt
     1321 acgacgattc cgagtccggt gttgggcggc gcgatggttt tgatgttcgg cctgattgcg
     1381 attgcgggcg tgcggatttt ggtcggccac ggcatccgca ggcgcgaggc ggtgattgcg
     1441 gcaacgtcgg tcggtttggg cttgggcgtg gggtttgagc cggaagtgtt taaaaacctg
     1501 cccgtcttgt tccaaaactc tatttccggc ggcggcatta cggcagtctt gctgaatttg
     1561 gtcttgccgg aagataaaac cgaggcggcg gtcaagtttg ataccgacca tttggaacac
     1621 tgattttgaa aacgaatgcc gtctgaaacg gaatccctgt ttcagacggc attgtttttg
     1681 aggcttacgc ttttttgttt ttcaatacgc gttgccggcg ggtttcgctt aataccattc
     1741 ctgcggacac ggagacgttc atgctttcga ccgtgccgaa catgggtatg gacaccagca
     1801 tgtcgcaatg ttcgcgtgtc aggcggcgca taccgtcgcc ttcgttgccc atcacccacg
     1861 ccgtgctgtc gggcagattg caatggtaaa ggtcggcgtc gccgcccatg tcggtgccga
     1921 tgatccaaat gccgtattct ttcaattcgc gcagggtgcg ggcgaggttg gttacggtga
     1981 tgtaggggac ggtttccgcc gcgccgcagg cgactttgct gacggtggcg ttcagccccg
     2041 cgcttttgtc tttcggcgcg atgacggcgt gtacgcccat tgcgtcggcg gtacgcaggc
     2101 acgcgccgag gttgtgcgga tcggtgatgc cgtcgagtat cagcagcagc ggcggttcgc
     2161 tcaagttttc caatatgcct tcgaggtgga cgtggttttt ggaggcatcg ataaatccga
     2221 ccacgccctg atggcgcgcg cctttgctga tggcgttgag gcggtcggca tcggcaaaat
     2281 atacgcggat gttttcgttt gccgcctttt ccaacacttc gcgcgtgcgt gcgtcggatt
     2341 tgccttcttg gatatagagt tcggtaatcg atttggggtt ttgccacaat cgggcgttga
     2401 cggcgtggaa gccgtagatg ggtctttggt ttgccataat ggtgtttgtc aaaaggtttc
     2461 agacggcatt atagcaattt gccggtatgc cgtctgaagg ggttaaaaca ggtatgcgat
     2521 gtatttcacc aacaggataa acaagatgga tacggcgcag ccgattttga acgccgtgcc
     2581 gacgacaagc cccaacagcg tacccaagcc cgctttacct gcctgaagca tattgcgccg
     2641 atcgatcagt tcgcctgccg ccgcgccgat aaaggggccg agtattagtc cgggaaggga
     2701 gaaaaatatg ccgatgatgc tgccggccaa tgcacctcgg acggcgagtt tgcctgcccc
     2761 agtgtatttt acccccaaca tgcctgccat atagtccgcc agtatgccgc caaggctgat
     2821 gagtccgacc gtccacaaga tgcctgcgcc gtagatttga tagccgccgg cataggcaag
     2881 cagccatgtt ccggcaaaca tcaatgccaa gccgggcagg gcgggataga cgatgcctgc
     2941 cgtgccgacg gctatcaggg cgagggcgag gatgacgagt agggcggtca tgggttcaac
     3001 cttttctttt gttttggaaa aaacggctta atacggcgcg gcattcttct tgcaggattc
     3061 cgccccgtat ggcggtgtgc gtattgaggc gtttgtcggc aaacaggttg acgatgctgc
     3121 ctgccgcgcc ggttttgggt tctgccgcac cgtagatcac gcgcctgatt cgtgcctgta
     3181 tcagtgcgga cgcgcacatg gcgcagggtt cgagggtgat gtagatgtcg catccgtcaa
     3241 ggcggtagtt ttgcatttcg ctgcctgcct gtgccaaggc gttgatttcg gcgtgtcggc
     3301 tgacattgca gtcggcaatg caggtgttgt gtgccgatgt gatgattttg ccgtctgaaa
     3361 cgatgactgc cccgacgggt atttcgccgt cggcggcgga ttgttctgct tggcgcagtg
     3421 cttcgcgcat gaagtatccc atttcttcct gcggcggaaa ggcggcgacg ggcggatggt
     3481 ttttcaactc ggcaagcagg cagtctttat gcgcttggga catttcttgc ggcggcgtgc
     3541 cgttcagcag cgactcgagt tgccacagcg tgcttttcgt gagggtcaaa cccgatgctt
     3601 tgagcagcag aaaggctttg accgaaccgt tttgccgcag ttcttcgagc gtacggatac
     3661 cgagcctgtg cagggcggcg acggttttgg gggcgagcgg cggcgtggcc agcatggttt
     3721 attcgccgaa aaaccgtttt gccgcctcaa tcaggcgcgt gcctgaagtg ccgtctgaaa
     3781 acgggtcggc aacgcagtct aaaggtgttt tgcgtaacca agtcagttgg cgtttggcaa
     3841 gttggcgcgt ggcggcaatg cctttttcga tgaaggtttg tctgtcggtt gcaccgtcga
     3901 ggtgttccca cgcctgacgg tagccgacgc agcggatggc gggggagtcg gcggtcaggc
     3961 cgggatagcg gcggcgcagg ttttctactt cgccgataaa gccctgttca agcatcaggt
     4021 ggaaacgcag ggcgatgttt tcgtgcagtc gggcgcggtc ttcgggaatc agggcggcgg
     4081 tgtagagttc gaagggcagg gtgtgttcgg gctgtccgtt caggtgcgcg ctcatcggcc
     4141 tgccggtcag gtaatagact tcaagggcgc gtccgatgcg ctggctgtcg ttcggtttca
     4201 gacggcatgc tgtttcaggg tcgacttttt gcagggtgcg gtagaggaaa tccaaaccgt
     4261 acatctgttt ttgttcgtcc aagtcggcac gcaggcaggc atcggcttcg ggcaaatcgt
     4321 tcaaaccttg ggtcagggcg cggaaataca tcattgtgcc gccgacaatc agcggacatt
     4381 tcccacgcgc cgtgatttcg ccggtcagac gcgtgcagtc ttcgacaaaa cgggcggcac
     4441 tgtatgtctg aacgggcgta atgatgtcga taaggtggtg cgggacaaag gcgcgttcgg
     4501 aggcggacgg tttcgccgtg ccgatgtcca taccggtata gagcagtgca gaatcgaggc
     4561 tgatgatttc gaccggaagg gtttcggcaa ttttgagggc gagcgcggtt ttgccgcagg
     4621 cggtcggccc gagcagggta aaggctttcg gggtcggcat aatgtttcag gtttggaaaa
     4681 atgcggatta tagcggaaag cgcgccgccc ttatattttg ctttgcggaa gcacgccgac
     4741 ggcaaggggc gggtttgccg tacgccttta taaaatgcgt tcaaaccgat gcgtcgggct
     4801 ttcaatatgc ccgcgcccga tgccgccttg tccgcaggca tcggcggcag cgtccgattt
     4861 ctcggggaac gcccgtcccg ggtatattta aaggttcggc ggtgcggcgt tttccggcgg
     4921 caaggcttca gacggcatct ccggcgcgtc cgttagacaa ggtgcgttct tgaggcgata
     4981 atggcgtttt gcttttttga aagccttgca atgtcccgaa acctgcttgt ccgctggctc
     5041 gccgtctgcc tcatcccctt ggcgacgctt gccgttttcg ccgccaatcc gcccgaagac
     5101 aaaccccagc atctgatcaa cggcatcatc cttgcctgcg aagcgacgtt tttgtttaaa
     5161 ttcgtcctct ttgaaaccat caagcatcat cttaaacaag ggtttgattt gaaacgtcaa
     5221 accatgtttc tgtttattcc gattgttttg ctggttgtgt atttgttcca ctatttcggc
     5281 gcgttttagc ttgttttccc gatggttatg aatacatacg cttttcctgt ctgttggatt
     5341 ttttgcaagg tcatcgacaa tttcggcgac atcggcgttt cgtggcggct cgcccgtgtt
     5401 ttgcaccgcg aactcggttg gcaggtgcat ttgtggacgg acgacgtgtc cgccttgcgc
     5461 gcgctttgtc ccgatttgcc cgatgttccc ttcgttcatc aggatattca tgtccgcact
     5521 tggcattccg atgcggcaga cattgatacc gcgcccgttc ccgatgccgt tatcgaaact
     5581 tttgcctgcg acctgcccga aaatgtgctg aacatcatcc gccgacacaa accgctttgg
     5641 ctgaattggg aatatttgag cgcggaggaa agcaatgaaa ggctgcacct gatgccttcg
     5701 ccgcaggagg gcgttcaaaa atatttttgg tttatgggtt tcagcgaaaa aagcggcggg
     5761 ttgatacgcg aacgcgatta ccgcgaagcc gtccgtttcg ataccgaagc cctgcgccgg
     5821 cggctggtgc tgcccgaaaa aaacgccccc gaatggctgc ttttcggcta tcggggcgat
     5881 gtttgggcaa agtggctgga catgtggcaa caggcaggca gcctgatgac cctactgctg
     5941 gcgggggcgc aaattatcga cagcctcaaa caaagcggcg ttattccgca aaacgccctg
     6001 caaaatgacg gcggtgtctt tcagacggca tccgtccgcc ttgtcaaaat cccgttcgtg
     6061 ccgcaacagg acttcgacaa attgctgcac ctcgccgact gcgccgtgat acgcggcgaa
     6121 gacagtttcg tgcgtaccca gcttgccgga aaaccctttt tttggcacat ctacccgcaa
     6181 gacgagaatg tccatctcga caaactccac gccttttggg ataaggcata cggcttctac
     6241 acgcccgaaa ccgcatcggt gcacctcctc ctttcggacg acctcaacgg cggagaggct
     6301 ttatccgcaa cacaacgcct cgaatgttgg caaaccctgc aacaacatca aaacggctgg
     6361 cggcaaggcg cggaggattg gagccgttat cttttcgggc agccttccgc atccgaaaaa
     6421 ctcgccgcct ttgtttcaaa gcatcaaaaa atacgctaga atagcgcgtt ttacgacaac
     6481 cgatttgatt ggaaaatcac aatgaaaaca gcacaagaac tgcgcgccgg caatgtattt
     6541 atggtcggca acgatcctat ggtcgttcaa aaaaccgaat acatcaaagg cggccgctct
     6601 tccgccaaag tcagcatgaa actgaaaaac ctgctgaccg gcgctgcttc cgaaaccatt
     6661 tacaaagccg acgacaaatt cgacgtggtc atcctgtccc gcaaaaactg tacgtacagc
     6721 tattttgccg acccgatgta cgtctttatg gacgaagaat tcaaccaata cgaaatcgaa
     6781 gccgacaaca tcggcgacgc gttgaaattc atcgttgacg gtatggaaga ccaatgcgaa
     6841 gttaccttct atgaaggcaa tcccatttct gtcgaactgc ccaccatcat cgtgcgcgaa
     6901 gtcgactaca ccgagcctgc cgtcaaaggc gatacttccg gcaaagtgat gaaaaccgcg
     6961 cgtctggtcg gcggcaccga aatccaagtg atgtcttaca tcgaaaacgg cgacaaagtc
     7021 gaaatcgata cccgtaccgg cgaattccgc aaacgcgcct gatatcctac ttgaaaaatg
     7081 ccgtctgaaa acgtttcaga cggcattttt ttatattcgc cccgtgtttg gattgaagta
     7141 tagtggatta aatttaaacc agtacagcgt tgcctcgcct tgccgtacta tctgtactgt
     7201 cttcggcttc gtcgccttgt cctgattttt gttaatccac tatagatatt tttttcgtaa
     7261 acgacaatac gcgtgatttt gccattttcg tcaaaatgga tgtctaaaaa cggtttgtcg
     7321 ggattgcgtt cacggttgga cagccggaag agtgattggt tttcaagcat ttctccgtgt
     7381 attttcagat agccgggcaa ttggccgatg tattggaaat tgccgccgag tccgttgttg
     7441 tgccggttca aataggcaga tgttgccgaa acttcaaaat aacgtgcgcg tttcggttcc
     7501 tcgccttgtt ctcggctttc ttgactgccc tgtgtcagca tcagcgtcgc ttctcggagg
     7561 cggcgttcct gttccattat ggcatcttgt gcctgccggc tgatagtgcg cgttccgtaa
     7621 cgcaagtctt cgattgccag catgattttc tgatggtagt cgggatcttc agggcggatg
     7681 tcgggaaaca gcagccgctg tatgttgtca tagccgccgc cgggtaagcc gaaacgggtt
     7741 tccagttcgt tatgggaaag ggcaaacaga cagtccgagt cgatgtgttc ggcgatttgc
     7801 tgtatgagat cgccaatgcc ggcggcgggg tgcaggccgg tgcaatttca cagtggcgcg
     7861 ctttctgcct gcttgacgtt atcaggggcg gatgcggccg gttgcaaatc ggattgggac
     7921 gcggaagacg aagcggcgga tgcgccgttt tctttcgttt tatcttcttt gctcggggaa
     7981 cgcgcggcca gcatgattgc ggcaagccat aagggaagtg atgacgtttt gtttttcatt
     8041 ctattgtttc caatattgaa aaggccgtct gaaaacctgc cgtttcattt ttcagacgga
     8101 ctgttgttaa tagaaccgag aaacccgtta ttgccgacaa ggttttcaac ctgtcttacc
     8161 cgacgcggta aaacgcaagg ctgcccaaaa ggttggggaa atgtttgacc tgtctgttgc
     8221 ccgtcatcac ggcgcgttcg aggacgcgga tattgttttt ggcgcacaac aaatcaaagt
     8281 ctttgagcgt gcaccaatgg atattgggcg tgtcgtacca atggtagggc atacgttcgg
     8341 aaaccggcat atgtccgccg agtgcgattt ggacgcggtt gcgccagtag ccgaaattcg
     8401 ggaaactgac gattgcctgt ttggcgaggc gcatcaggca gcgcaggatt ttttcggtat
     8461 tctgcatcgc ttggatggtt tggctcaaaa caatcacatc aaaactttga tcgttgaatg
     8521 cggtcaaccc ttcttccaaa tcggcttgga taacatttac gccgcgcgac atcgcagcaa
     8581 tcacgttgtc ggtgtcgatt tcgatgccgt agccgctgca ttttttgtgt tccgccaaag
     8641 ccgccagcaa ttcgccgtcg ccgcagccca agtctaagac gcggctgcct tcgggtatcc
     8701 ggtcgtaaat cagttgcaaa tcatcgcgca ggttcataat cggcaatcct tgtcaacatt
     8761 gttcatataa gccgttacgg cgcgcatata ggcttcgtct tccattaaaa aggcatcgtg
     8821 cccgtgtgcg gacttgactt cgatatactg cacggatttt tgggcggcaa tcagtgcctt
     8881 gaccagttcg tgcgaacgtt cgggcgcgaa acgccagtcg gtgctgaagc tggcgacaaa
     8941 gaattttgcc tgcacatcct gcacggcgcg agtcaggctg ttgccgaaat ccgccgccgg
     9001 atcgaaatag tccaaagctt tggtcatcag caaatatgta ttagcatcaa accgcccgac
     9061 gaatttgtcg ccttgatagc ggagatagga ttctacttca aattcaacgc tatagccgta
     9121 ttgatagccg ttggaacgca aatcgcgtcc gaattttttg cccaaaccgt cttcggcaag
     9181 atacgtaatg tgtcccatca tacgggcaat ccgcaaaccg cgcgcgggaa cggtgttgtg
     9241 gctgcggtaa tgtccttcat tgaaatcggg gtcggtcaaa atcgcctgac gtgctacatc
     9301 attaaacgcg atattttgcg cggacagttt cggcgcagac gcaatcacca aggcgtggcg
     9361 cacacgttcg ggataggaaa tcgcccactg caaagcctgc atgccgccca agctgccgcc
     9421 gacaaccgcc gcccattgtt cgatgccgag ataatcggca agcgcggctt gtgattttac
     9481 ccagtccttc accgtaacca tcggaaaatc cgcgccgtat tccctgcccg tttcaggatt
     9541 gatcgacaaa ggcccgctgc tgccgtcgca gccgcccaga ttgttcaacc cgaccacgaa
     9601 aaaacgttcc gtatcaatcg gttttccggg accgaccata ttgtcccacc agcccgtata
     9661 tttatcctcc gccgaatgcc tgcccgcaac gtgatggttg cccgacagcg cgtggcagat
     9721 taaaaccgca ttgttttttt cagcattcag ctcgccgtag gtttcaatca tcagatcgaa
     9781 acgcggcaaa gttttaccgt tttccaaaac cagcggcatt tcaaacggaa ttttttgggg
     9841 cgttacaatg cccaccgagg tattttgact catatcctgt tccaacaaat gcggcgaaaa
     9901 gcgttattat atcgcaaacg gcatgacttt ttgacacggt cggacaagca gcctgacgcg
     9961 ctgaaacatc ctttcccatg ccgccgcgcc cgaaccatac ttttcgcttc ccgacatgat
    10021 aggcagactt ttccgtattt ttttcttttt cgcacttgcc gcgctgatta tcaaccgcct
    10081 ctttagccgc aggcagaaac gtgtcctgcg cgaagtcgcc gaaatcagcg catgggtact
    10141 gctcggtgca gccgccgcga cgctgttttg gtatctgttt atgctgtatt tcaaacacat
    10201 tccggattcg tatcgacgga aaaatgccgt ctgaaacgca tttttctgtt tcagacggca
    10261 tatttgaaaa gggcttgcgg taggaggggc tttacaaccg aagcaggaag ggcagggggt
    10321 cagcgttggc gcgatttaaa acgcggattg cttttgcaga tgacgtacac tttgcccctg
    10381 cgtctgacga tttggcagtc gcggtggcgt tgtttggcgg ttttgagcga agacagaacc
    10441 tgcattattt gtcctttcta aacgatgaca tgacggattg gaaacgttgg ttgaacttgc
    10501 tggcacggcc ttcggtattg acgttacgct gtttgccggt atagacggga tgtgaggaag
    10561 aggaagtatc taaggaaaag agcggatatt ctttgccgtc tgtccaaacc attgtttttc
    10621 cgtgcgttcc ggcgcaagaa cgaatcagcc agccttcatt ggcactgctg tcgaaaaaaa
    10681 ggacggttcg ataattgtca ggatgaatat tcggtttcat atattgcctt gcttgaagtg
    10741 ttataacata acaaacttta tcataattta gaaggcttgt acaagaaaag cgtatagttt
    10801 ttgctaaata ttaaaaattt tcatcataaa cctgaaaatt ttgaaattga ctcatattcg
    10861 gtgcaacctt atcataccgc ctgaaacatc atgtaaaaga taacgaaggt atgcagatat
    10921 gaattatgca aaagaaatca atgcgttaaa caacagcctt tccgatttga aaggcaacat
    10981 caacgtttcg tttgaatttt tcccgccgaa aaacgaacaa atggaaacga tgctgtggga
    11041 ttccatccac cgtctgcaaa ccctgcatcc caagttcgta tccgtaactt acggcgcaaa
    11101 ctccggcgac cgcgaccgca cgcacggcat cgtcaaacgc atcaaacagg aaaccggttt
    11161 ggaagcagct ccgcacctga ccggcatcga tgcatcccac gacgaattgc gccaaatcgc
    11221 caaagactat tgggacagcg gtatccgccg catcgttgcc ctgcgtggcg acgagccggc
    11281 cggttatgag aaaaaaccgt tttacgccga agacttggtt aagctattac gctccgtcgc
    11341 cgacttcgac atctctgtag cagcataccc cgaagtacat cccgaagcga aatccgcaca
    11401 agccgacctg attaatctga agcgcaaaat cgatgcgggc gcgaaccacg tcatcaccca
    11461 attcttcttc gatgtggaac gctacctgcg cttccgcgac cgctgcgtga tgttgggcat
    11521 cgacgtcgaa atcgttcccg gcatcctgcc tgttaccaac ttcaagcaac tcggcaaaat
    11581 ggcacaagta accaacgtca aaatcccgaa atggctgtcg caaatgtatg aaggcttgga
    11641 cgacgaccaa ggcacgcgca acctcgtcgc cgccagtatc gccatcgata tggttaaagt
    11701 cctgtcccgc gaaggcgtga aagatttcca cttctacacg ctcaaccgca gcgagctgac
    11761 ttacgccatc tgccatattt taggcgcgcg cccttaaagc cgaaaacggc ttcagacagc
    11821 atccgaggcc gtctgaaaag caaaacgccg ccctatccga gccattctga tttacaatac
    11881 cggccgattc ggattgaacc ggtccttaca aaatccaact ggagagttca acatgacaac
    11941 attacatttc tcaggcttcc cgcgtgtcgg cgccttccgt gaattgaaat tcgcccaaga
    12001 aaaatactgg cgcaaagaga tcagtgaaca agagctgctc gacgttgcca aagacctgcg
    12061 cgagaaaaac tggaaacacc aagctgctgc caacgctgat tacgttgctg taggtgattt
    12121 cactttctac gatcacatcc tcgacctgca agttgccacc ggcgcgattc ccgcccgttt
    12181 cggcttcgat agccaaaacc tgcctttgga acaattcttc caactggcgc gcggcaacaa
    12241 agaccaattc gccatcgaaa tgaccaaatg gttcgacacc aactaccact acctggtgcc
    12301 tgaattccat gccgataccg aattcaaagc caacgccaaa cactacgttc aacaactgca
    12361 agaagcccaa actttgggct tgaaagccaa accgaccgtt gtcggtcctt tgactttcct
    12421 gtgggtgggt aaagaaaaag gctccgttga attcgaccgt ctgagcctgt tgcctaaact
    12481 gttgcctgtt tacgttgaaa tcctgactgc tttggttgaa gccggtgccg agtggattca
    12541 aatcgacgag cctgctttgg ctgtcgatct gcctaaagaa tgggtagaag catacaaaga
    12601 cgtttacgct actttgaaca aagtaagcgc caaaatcctg ttgggcactt acttcggttc
    12661 tgttgccgaa cacgccgcat tgttgaaatc cctgcctgtt gacggcctgc acatcgactt
    12721 ggtacgcgca cccgagcaac tggacgcgtt cgccggctac gacaaagtcc tgtctgccgg
    12781 cgtgattgac ggccgcaaca tttggcgcgc caacctgaac aaagttttgg aaaccgtcgg
    12841 gcctctgcaa gccaaactgg gcgagcgttt gtggatttcc agctcttgct cgctgctgca
    12901 cactccgttt gacttgtcaa tcgaagaaaa actgaaagcc aacaaacccg acctgtactc
    12961 ttggttggca ttcactctgc aaaaaaccca agaattgcgt gttctgaaag ccgcattgaa
    13021 tgaaggccgt gattctgttg ccgaagaact ggccgccagc caagctgctg ccgactcccg
    13081 tgccaacagc agtgaaatcc accgtgcaga cgttgccaaa cgcttggccg acctgcctgt
    13141 caacgcaggc caacgcaaat caccgtttgc cgaccgtatc aaagcgcaac aagcatggtt
    13201 gaacctgcct ctgctgccga cgaccaacat tggttctttc ccgcaaacta ccgaaatccg
    13261 ccaagcacgc gcagccttca aaaaaggcga actgtctgcc tccgattacg aagccgcaat
    13321 gaaaaaagaa atcgccttgg tggttgaaga gcaagaaaaa ctggacttgg acgtactggt
    13381 gcacggcgaa gccgagcgta acgacatggt cgaatacttc ggcgaactgt tgagcggttt
    13441 tgcattcacc caatacggct gggtgcaaag ctacggctca cgctgcgtta aaccacctat
    13501 catcttcggt gacgtaagcc gtcctgaagc tatgactgtc gcttggtcta cttacgcaca
    13561 aaacctgacc aaacgcccga tgaaaggtat gttgaccggt cctgtaacca ttctgcaatg
    13621 gtctttcgtc cgcaacgata ttccgcgcgc taccgtgtgc aaacaaatcg cactggctct
    13681 gaacgacgaa gtattggatc tggaaaaagc cggcattaaa gtcatccaaa ttgacgaacc
    13741 tgccatccgc gaaggtcttc ctttgaaacg tgccgattgg gatgcctacc tgaactgggc
    13801 gggcgaatcc ttccgcctat cctctgcagg ttgcgaagac agcactcaaa tccacactca
    13861 tatgtgctac tctgagttca acgacatcct gcctgcgatt gctgcaatgg atgcggacgt
    13921 gatcaccatt gagacttcac gttccgacat ggaactcttg gctgcgttcg gcgaattcaa
    13981 atacccgaac gacatcggcc cgggcgttta cgacatccac agcccgcgcg taccgacaga
    14041 agccgaagtg gaacacctgt tgcgcaaagc cattgaggtt gttccggttg agcgtctgtg
    14101 ggttaacccg gactgcggcc tgaaaacacg cggctggaaa gaaactctgg aacagcttca
    14161 agtaatgatg aacgtaaccc gcaaactgcg tgccgaattg gcgaaataag ccgagaccgt
    14221 atgaacaaat gccgtctgaa agcctttcag acggcatttt gtcctgagtt gcggcgcagg
    14281 ggagcagttg ccggaaaatc ttttcattgc agcttgtttt tctctaattc ggctttatat
    14341 gttgggaaca ggcaaatcgg agttgtattt gatagtttta aataatttat attatttaaa
    14401 ctataaatta tacaaatcat tttgcatggg gtagaatgcg cggcgattca caattatttc
    14461 tcaaaccaat ctattaagga gctcgaaatg gctttacaag atcgtaccgg tcaaaaagta
    14521 ccttccgtag tattccgcac ccgcgtcggc gacacttgga aagatgtgtc taccgatgat
    14581 ttgttcaaag gcaaaaaagt agtcgtattc tccctgcccg gtgcatttac cccgacttgt
    14641 tcttcttcac acctgccgcg ttacaacgaa ttgttcggcg cgttcaaaga aaacggcgtt
    14701 gacgcaatct gctgcgtatc tgtaaacgat actttcgtaa tgaacgcttg ggctgccgaa
    14761 gaagaatcag acaacatcta catgattcct gacggcaacg gcgaatttac cgaaggtatg
    14821 ggtatgctgg tcggtaaaga agacttgggc ttcggcaaac gctcttggcg ttactccatg
    14881 ctggttaacg acggcgtggt tgaaaaaatg ttcatcgaac ctgaagaacc gggcgatcct
    14941 ttcaaagtat ccgatgcaga tactatgctg aaattcgttg ctcccgattg gaaggctcaa
    15001 gagtctgtgg caattttcac taaaccaggt tgccaattct gtgccaaagt caaacaagct
    15061 ttgcaagaca aaggtttgtc ttacgaagaa atcgtattgg gcaaagatgc aaccgttact
    15121 tccgttcgcg ctattaccgg caagatgact gcccctcaag tcttcatcgg cggcaaatac
    15181 atcggcggca gcgaagattt ggaagcttac ttggctaaaa actgatagct gtttgcttaa
    15241 ggcagtttga ttaaactgtc tgatataccg gatagagtta ttcgggcggt tctatactgc
    15301 cgctccgaat aactctatat ttacaagaag atttggatat tgtcgcactc aatcgaaatt
    15361 ttgtttttat ttatctgaat aatgtttttg attgggaaga tatttaaatg ctgtctgaaa
    15421 ccgatatgtt ctgtgtcggc aatgtttcag acgaaaacgg aaggacaaag attatgaaaa
    15481 aaattcaagc ggatatcgtc gtaatcggcg gcggtactgc cggtatgggt gcgtttcgca
    15541 atgcccgttt acattcggat aatgtttacc tgattgaaaa caatgtgttc ggcacaacct
    15601 gcgcgcgcgt gggctgtatg ccttccaaac tcttgattgc cgccgcagaa gcgcgtcacc
    15661 acgcattgca taccgacccc ttcggcgtgc atttggacaa agacagcatc gtcgtcaacg
    15721 gagaagaggt tatgcggcgc gtcaaatccg agcgcgaccg ttttgtcggc tttgtcgtta
    15781 ccgatgtgga agaatggcct gccgacaagc gcatcatggg ctcggctaaa ttcatcgacg
    15841 agcataccgt ccaaatcgac gaccatatcc aaattgcggc aaaaagtttc gtgattgcta
    15901 ccggttcgcg tcccgtcatc ctgccgcagt ggcagtcttt gggcgaccgt ttgattatca
    15961 acgacgacgt tttctcgtgg gatacgctgc ctaagcgcgt tgccgtgttc gggccgggcg
    16021 ttatcggttt ggaactgggt caggcattgc accgtttggg tgtgaaagtt gaaattttcg
    16081 gtttgggcgg aatcatcggc ggcatttccg accccgttgt ttcagacgag gcgaaagccg
    16141 tgttcggaga agaattgaaa ctgcatctgg atgctaaaac cgaggtcaaa ctcgacgcag
    16201 acggcaatgt agaagtccat tgggagcagg atggcgaaaa aggcgtattt gttgccgaat
    16261 atatgttggc agccgtaggt cgccgtccga acgttgacaa tatcggtttg gaaaacatca
    16321 atatcgacaa agacgcgcgc ggcgtacccg ttgccgatcc gctgaccatg cagaccagca
    16381 ttccgcatat ctttattgcg ggcgacgcgt ccaaccaact gcccctgctg catgaagccg
    16441 ccgaccaagg taagattgcc ggcgataacg cgggccgcta cccgaatatc ggcagcggtt
    16501 tgcgccgcag caccattggc gtggtgttta ccagtccgca aatcggcttt gtcggtttga
    16561 aatacgcgca ggttgccgcg caataccaaa ccgacgaatt tgtcatcggc gaagtatcgt
    16621 tcaaaaatca aggtcgcagc cgcgtgatgc tggtaaacaa aggccatatg cgcctgtatg
    16681 ccgaaaaagc caccggccgc tttatcggcg cggaaatcgt aggccctgcc gccgaacatt
    16741 tggctcactt gttggcatgg gcgcatcaaa tgaagatgac cgttccgcaa atgctggata
    16801 tgccgttcta ccatcctgtt atcgaggaag gtctgcgtac cgcgttgcgc gatgccgatg
    16861 cgaaattgaa agcctgaccg atatggcaaa acaatgccgc ctgaaatttt tttcagacgg
    16921 cattttattt ttggggatgg ggttgggggt cggatgctga taccgtgtcg ggaagggagc
    16981 ggcaaaacta aaaatctttc ttttaatctg ctgtttccac gcgtgtttgt caaaatctat
    17041 cagtttgttt ttaaaataca ctgttcaaaa tgggataaaa caggtaaatt aacgttgatg
    17101 taacccagtg tagcaatggg tttacggttt ttgagtcgat atataactac agaggaattg
    17161 actatgtctg tcaaactgcg tcctgtttat ctggatttgc cgaacatccg tctgccgata
    17221 cccgggatag tttccatcct tcaccgcatt agcggggtcg ggctgtttat tatgctgcct
    17281 ttcctgctgt attttctgtc cggtaccctg agtcaagagt ctgcatttga aacttaccgt
    17341 gccattgttt cccatccttt ggtcaagctg gttttaatcg gtatattgtg ggcttatctg
    17401 caccattctc tcgccggtat ccgcttttta tttttggatg cgcacaaagg ccttgagctg
    17461 aatactgcgc gcaataccgc taaagccgta tttgcttctg cattggtttt gactgtcgtt
    17521 ttgggagcgt tgttatggta gaacgtaaat tgaccggtgc ccattacggt ttgcgcgatt
    17581 gggtaatgca gcgtgcgact gcggttatta tgttgattta taccgttgca cttttagtgg
    17641 ttctatttgc cctgcctaaa gaatatccgg catggcaggc attttttagt caagcttggg
    17701 taaaagtatt tacccaagtg agctttatcg ccgtattctt gcacgcttgg gtgggtatcc
    17761 gcgatttgtg gatggactat atcaaaccct tcggcgtgcg tttgtttttg caggttgcca
    17821 ccattgtctg gctggtcggc tgcctcgtgt attcagttaa agtgatttgg gggtaagtat
    17881 gggttttcct gttcgcaagt ttgatgccgt gattgtcggc ggtggcggtg caggtttacg
    17941 tgcagccctc caattatcca aatccggttt gaattgtgcc gttttgtcta aagtgttccc
    18001 gacccgctcg cataccgtag cggcgcaggg cggtatttcc gcctctctgg gtaatgtgca
    18061 ggaggaccgt tgggactggc acatgtacga taccgtgaaa ggttccgact ggctgggcga
    18121 ccaagatgcg attgagttta tgtgtcgcgc tgcgcctgaa gcggtgattg agttggaaca
    18181 catgggtatg ccttttgacc gcgttgaaag cggcaaaatt tatcagcgtc ctttcggcgg
    18241 acatactgcc gaacatggta aacgtgcggt agaacgtgca tgtgcggttg ccgaccgtac
    18301 cggtcatgcg atgttgcata ctttgtacca acaaaacgtc cgtgccaata cacaattctt
    18361 tgtggaatgg acggcgcaag atttgattcg tgatgaaaac ggcgatgtcg tcggcgtaac
    18421 cgccatggaa atggaaacgg gcgaagttta tattttccac gccaaggccg tgatgtttgc
    18481 taccggtggc ggcggtcgta tttatgcttc ttctaccaat gcttatatga ataccggtga
    18541 cggtttgggc atttgcgccc gtgcgggcat tccgttggaa gatatggaat tctggcaatt
    18601 ccacccgacc ggcgtggcgg gtgcgggcgt gttgattacc gaaggcgtac gcggcgaggg
    18661 cggtattctg ttgaacgccg acggcgaacg ctttatggaa cgctatgcgc cgaccgtaaa
    18721 agacttggct tctcgcgacg tggtttcacg cgcgatggcg atggaaatct atgaaggtcg
    18781 cggctgtggt aaaaacaaag accacgtctt actgaaaatc gaccatatcg gtgcagaaaa
    18841 aattatggaa aaactgccgg gcatccgcga gatttccatt cagtttgccg gtatcgatcc
    18901 gattaaagac ccgattccgg ttgtgccgac tacccactat atgatgggcg gcattccgac
    18961 caattatcac ggtgaagttg ttgttccgca aggcgacgag tacgaagtac ctgtaaaagg
    19021 cctgtatgcc gcaggtgagt gcgcctgtgc ttccgtacac ggtgcgaacc gtttgggtac
    19081 gaactccctg ctggacttgg tggtgttcgg caaagctgcc ggtgacagca tgattaaatt
    19141 catcaaagag caaagcgatt ggaaacctct gcctgctaat gccggtgagt tgacccgcca
    19201 acgtatcgag cgtttggaca gtcaaaccga tggtgaaaac gttgatgcat tgcgtcgcga
    19261 actgcaacgc tccgtacaac tgcacgccgg cgtgttccgt accgatgaga ttctgagcaa
    19321 aggcgttcaa gaaatcatgg cgattgccga gcgtgtgaaa cgtaccgaaa tcaaagacaa
    19381 gagcaaagtg tggaataccg cgcgtatcga agctttggaa ttggataacc tgattgaagt
    19441 ggcgaaagcg actttggtgt ctgccgaagc acgtaaagaa tcacgcggtg cgcacgcttc
    19501 agacgaccat cctgagcgcg atgacgaaaa ttggatgaaa cacaccctgt atcattcgga
    19561 caccaatacc ttgtcttaca aaccggtaca caccaagcct ttgagcgtgg aatacatcaa
    19621 accggccaaa cgcgtttatt gatgcgtttt cagacggtct tcgcctcaaa ggtcgtctga
    19681 aacctaacca tacccacatt gaactgcttg aatttataat acaaaatcat tgggcagttg
    19741 atgagaaaag gaacacttct catggaaaaa atgagttttg aaatttaccg ttacaacccg
    19801 gacgttgatg ccaagcctta tatgcagcgt tacgagttgg aattggaacc gaccgacgtg
    19861 aaacttttgg acgctttggt acgcctgaaa gcacaagacg ataccttgtc tttccgccgc
    19921 tcctgccgcg aagggatttg cggatcggac ggtatgaaca tcaacggcaa aaacggcttg
    19981 gcgtgtctga ccgatttacg cagcttgaaa cagcctgtca aaatccgccc gctgcccggt
    20041 ctgcccgtca tccgcgacct gattgtggat atgacccagt tcttcaaaca ataccattcc
    20101 gtcaaacctt atgttgttaa cgacaatccg attgatgcgg acaaagagcg tctgcaaact
    20161 caggaagagc gcaaagagtt ggatggtttg tacgaatgta ttttgtgcgc ctgctgttcg
    20221 accgcctgtc cgtcattctg gtggaatccc gacaaattcg tcggtccgtc cggcttgctg
    20281 aacgcctacc gcttcattgc ggacagccga gataccatca ctaatgagcg tttggataat
    20341 ttgaacgatc cgtaccgttt gttccgctgc cacaccatta tgaactgcgt ggacgtatgt
    20401 cccaaacact tgaatccgac ccgggccatc ggtaagatta aagagattat gttgaaacgg
    20461 gttgtttaag aaatgatggt ttttgacgac attgccaaac ggaaaatccg ttttcaaacc
    20521 cgccggggat tgttggaact agatttaatc ttcggcaggt ttatggaaaa agaattcgag
    20581 catttgagcg ataaagagct gtccgagttt tccgaaatcc ttgaatttca agatcaagaa
    20641 ttgcttgcct tgattcacgg gcattcggaa acggacaaag ggcaccttat cccgatgctt
    20701 gaaaaaatca gacgggcatg aggcatctga aatttgtctg agggcagatt tcaaaatgca
    20761 aaggccgtct gaaagcaaag aacgtgctgc ggatgcagta acgtgggtta taacttgcaa
    20821 aggagcgata atatgtccaa atcaatcaaa ctcaacgtac cgggtcgggc aggtttggag
    20881 ctgccggtat tggaagccag catcgggcat gatgtggttg acattcgggg gctgacaaaa
    20941 aatacaggtt tgtttgcctt cgaccccgga tttgtttcaa ccgcaagctg tgagtctaaa
    21001 attacttaca tcgacggcga tcaaggcttg ctttattatc gcggataccc catcgagcag
    21061 ctggccgaaa agtccgatta tttggaagtc tgctacctgt tgatttacgg cgaactgccg
    21121 actcccgagc aaaaggcaga atttgacaat acagtccgcc gccacacgat ggtgcatgaa
    21181 cagctgactt ggttcttccg gggtttccgc cgcgacgcgc atccgatggc gatgatggtc
    21241 ggcgtggtcg gcgcgctgtc tgcgttctac caagacagct tggacattac taatcccgaa
    21301 caccgcaaaa tcgcgattta ccgccttatt tccaaaattc cgaccattgc ggcaatgtgt
    21361 taccgctatt caaacggtct gccgttcaat tatccgaaga ataatctttc ttattcagaa
    21421 aacttccttc atatgatgtt tgccacaccg tgtgaagact acaaacctaa tcccgttttg
    21481 gcacgcgcgc tcgaccgcat ctttattttg catgccgacc acgagcaaaa cgcctcaact
    21541 tcaaccgtcc gtctggcagg ctcttcgggt gcgaacccgt ttgcctgtat tgctgccggt
    21601 atcgcctgcc tgtggggtgc ttcgcacggc ggtgcgaacg aagccgtgtt gaaaatgctg
    21661 gatgaaatcg gcgatgtgtc caatgttgcc gcatacatgg aaggcgtgaa acagcgcaaa
    21721 taccgcctga tgggcttcgg acaccgggta taccgcaata tggatccgcg tgccagcatt
    21781 atgcgcgaaa cctgctatga agttttgaag gaattgggct tggaagacag tccgaaattc
    21841 aaactggcga tggaattgga acagattgcg ctgaaagacc cgttctttat cgaacgcaaa
    21901 ctgtatccca atgtcgattt ctattccggc atcgtcctgt ccgcgctggg catcccgacc
    21961 gaaatgttta ccgtcatctt cgccctgtcg cgcagcgtgg gctggatttc gcactggcac
    22021 gagatgatta gcgatccttc gctgaaaatc ggccgcccgc gccagcttta taccggttcg
    22081 gaacgccgcg attatgtgcc ggcaggcgag aggtaacata cattaatata ttgtcaaaca
    22141 ggcaatatca gagaaccgga ttgtttcctg aatccgtctg attgtagtcg gatgaaatca
    22201 agacaagcaa tccggtttaa aatagggtag aataaaatgt cttttcagac ggcatcagtt
    22261 cagccgtcag gacgcggact tctacccttt gtttatattt taaagaaaag agcgcacgcc
    22321 atgatggacg aaaaactcaa tttctcttat ctgttcggtt cgaacgcacc ctacattgag
    22381 gaattgtacg aggctttttt ggaaaacccc gatgcggttg atgaaaaatg gaagcagtat
    22441 ttcaccgatt tgagcaaaca gccggggacg gttgctgtcg atgtcgcaca cacaccgatt
    22501 cgcgaatcat ttgttacttt ggcgaaaaag aaaattgcat ctgccgttgc gggcggtgcg
    22561 gatgaggcaa tgctgaaaaa gcaagtcagc gttttacggc tgatttctgc ctatcgtatc
    22621 caaggcgtgg gtgcggccca acttgatccg ctcaaacgta tccctccgcg cgatattgaa
    22681 gccctcgatc cgaaattcca cggtctgtca gatgccgata tggcgcttcg attcaatatg
    22741 ggcgagggtg attttgccaa tcgcggcaaa ctgcccttgt cccaaatcat cagcaacctc
    22801 aaacaaacct actgcggcca catcgcattg gaatatatct atattcccaa taccgaagag
    22861 cgccgttggg tacgcaacta ttttgaaagc gtattgtcca caccgcatta caatgccgat
    22921 caaaaacgcc gtatcttgaa agagatgacc gctgccgaga ctttggaacg ttatctgcat
    22981 accaaatatg tcggtcagaa acgtttcggt gtcgaaggcg gcgaaagcgc gattgccggt
    23041 ttgaactacc tgattcaaaa cgccggtaag gacggcgtgg aagaagtcat catcggtatg
    23101 gcgcaccgtg gccgtctgaa tgttttggtg aacattttgg gcaaaaaacc cggcgatttg
    23161 tttgccgaat ttgaaggccg tgccgaaatc aaactgccta gtggcgacgt gaaataccat
    23221 atgggcttca gctccgatat tgccacccct cacggcccga tgcacgtttc tttggcgttc
    23281 aacccgtcac acttggaaat cgtcaatccg gtagtggaag gttctgcacg cgccaaacaa
    23341 aaacgtttgg gcgaaaacgg ccgcgataaa gtcttgccgg tattgattca cggcgattcc
    23401 gcatttatcg gtttgggtgt caaccaagca acattcaacc tgtctaaaac gcgcggttat
    23461 accacaggcg gtacggtcca tatcgtcatc aacaaccaaa tcggctttac cacttccgat
    23521 atccgcgata cccgttcaac cgtacactgt accgatatcg caaaaatggt ttccgctccg
    23581 gttatccatg tgaacggcga tgatcccgaa cgcgtttgct ttgccattca agccgctttg
    23641 gattaccgca aaaaattcca taaagacatc gtaatcgacg ttgtctgcta ccgtaaatgg
    23701 ggtcacaacg agggcgatga tccgactttg acccaaccga tgatgtacaa aaaagtatcg
    23761 caacatccgg gtgcgcgtgc tttgtacacc gagcaactga ttgccgaagg cgtggtaact
    23821 caagttgagg ccgacggtta catccaagcc taccgtgatg ctttggacaa aggcgaacat
    23881 gttgagcaaa caacgttgag caacttccaa cgcacacaaa tcgactggag caaataccaa
    23941 ggtaaagatt ggcgcgaaaa aatcgaaacc ggtttgcctg ccgccgacat tgagcgtctt
    24001 actgagaaat tcaccgctgt gccggaaggc tttgccctgc atccgactgc aaaacgtgtg
    24061 atcgaagcgc gtaaagccat ggcatccggc aaacaagcca tcgactgggg tatggccgaa
    24121 accctcgcat acgcaagcct gctgaccaaa ggtcatggcg tgcgtatctc cggtgaggac
    24181 tccggccgcg gtacattctc acaccgtcat gccgttctgc acgatcaaaa acgcgaaaaa
    24241 tgggacgacg gtacttatgt tcctcttcgc aacatgggcg aaggcttggg cgagttcctg
    24301 gttatcgact ctatcttgaa cgaagaagcc gtgatggcgt tcgagtacgg ctttgcctgc
    24361 tccgctcctg acaagctgac catttgggaa gctcaattcg gtgacttcgc caacggcgcg
    24421 caagtaacta ttgatcaatt cctgtcttca ggcgaaacca agtggggtcg cttgtgcggt
    24481 ctgaccacca tcctgccgca cggctacgac ggtcaaggcc ctgaacactc ttctgcacgc
    24541 gtagaacgtt ggttgcaact gtgttctgag aacaatatgc aagtcatcat gccgtctgaa
    24601 gcgtcgcaaa tgttccatct cttgcaacgc caagtcttgg gttcataccg caaaccgctg
    24661 gtgattttca tgtccaaacg cctgttgcgc ttcaaaggtg caatgagccc gctggaaaac
    24721 ttcaccgaag gttcgacttt ccgtccggtt attggtgata ccgccgaacg cgcaagcaac
    24781 gacagcgtga aacgcgtggt attgtgtgcc ggtcaggttt actatgactt ggaagccggt
    24841 cgagccgaac gtaaactgga agatgatgtc gctatcgtcc gcgttgagca gctgtatccg
    24901 ttcccatacg acgaggttaa agccgaactg gcgaaatatc cgaacgcaaa atctgtggtt
    24961 tgggcgcaag aagagccgaa aaaccaaggc gcgttctacc aaatccgcca ccgcatcgaa
    25021 gacgttatca gcgaagagca aaaactgtct tatgccggcc gtccaagcag cgcatcgcct
    25081 gcagtgggct actcaagcaa acacattgct caattgaaac aattggttga agacgctttg
    25141 gcgttataaa ccaagtagca ttccgtctga gtctgctcag gcggaatgcc catatgcaga
    25201 attaaaaaca cacaacaggc cgtctgaaag ggccattgga gacacaaaat gattattgat
    25261 gtaaaagtac ctatgctgtc tgaaagcgta tctgaaggca cactcttgga atggaagaaa
    25321 aaagttggcg aagcagttgc ccgtgacgaa atcctgatcg atatcgaaac ggacaaagtg
    25381 gttttggaag taccttctcc acaagccggc gtattggttg aaatcgtagc gcaagacggt
    25441 gaaaccgttg ttgccgacca agttttggcc cgtatcgata cagctgctac tgtcgctgct
    25501 gaagcacctg cagccgctcc tgccgaagct gccccggctg ccgttcctgc tgctgcacaa
    25561 aacaacgccg ccatgcctgc cgctgcaaaa ctggctgccg agaccggtgt tgacgtgaac
    25621 gtattgcaag gttccggccg tgacggtcgc gtattgaaag aagacgtaca aaatgccgct
    25681 gccaaacctg ccgcagccgt ggcccctgcc gttgcacttc ctgccggcgc acgtcctgaa
    25741 gaacgcgtac caatgagccg cctgcgtgcc cgtgttgcag aacgcctctt ggcttctcaa
    25801 caagaaaacg ccattctgac tacattcaac gaagtcaaca tgaaaccgat catggacttg
    25861 cgtgcgaagt acaaagaaaa attcgagaaa gaacatggcg taaaactggg ctttatgtcc
    25921 ttcttcgtta aagccgctgt tacagccctg aaaaaatacc cggttgtgaa tgcttctgtt
    25981 gacggcaaag acattgtgta ccacggctac ttcgacatcg gtatcgcaat tggcagccca
    26041 cgcggtttgg ttgtaccaat cctgcgcgat gccgaccaaa tgagcattgc cgacatcgaa
    26101 caagcaattg ttgattacgc gaaaaaagcc aaagacggca aaatcgctat cgaagacctg
    26161 accggcggta cattcagtat taccaacggc ggtactttcg gttctatgat gtccaccccg
    26221 atcatcaacc cgcctcaatc tgcgattttg ggtatgcatg ccactaaaga gcgcgctgtg
    26281 gttgaaaacg gccaagttgt tgtccgtcca atgatgtatc tggctctgtc ttacgaccac
    26341 cgtatcattg acggccgcga agctgtattg accttggtag ccattaaaga cgcgttggaa
    26401 gacccggtcc gcctgttgtt ggatctgtaa tcgtttcaga cggcttttta tttgttaatg
    26461 aaaaggccgt ctgaattttt atttagatgt agcgtaatgt agtatcgtgc tacaataggc
    26521 tcaacgaacg attgaggccg tctgaaacat ttgattcgaa tgaatcggca gatatggact
    26581 ttcagacggc tttttcttaa aaccatcaaa acgcagtcat tcaaaataaa aaaagaaacg
    26641 aaaagtatcg tttttatttt gagatactgc taaaagcaaa ggatgacacg atgtctcaat
    26701 atgatgtagt agtgattggt gcaggtccgg gcggatacgt tgccgccatc cgtgccgcac
    26761 aactgggttt caaaactgcc tgtgtcgatg caggcgttaa caaagcaggc aatgcccctg
    26821 cattgggcgg tacttgcttg aacgtaggct gtatcccttc taaagccctg ttgcaatcca
    26881 gcgaacattt ccacgctgcg caacacgatt ttgccgaaca cggtatcact gtcggcgacg
    26941 taaaattcga cgcggccaaa atgattgagc gcaaagatgc catcgtgacc aaactgaccg
    27001 gcggcgtgaa attcctgttc caaaaaaaca aagtaaccag cctgttcggt actgcttcct
    27061 ttgccggtaa aaatggcgat gcttaccaaa ttgaagtcga taacaaaggc gagaaaaccg
    27121 ttatcgaagc caaacacgtc atcgtagcaa ccggttccgt accgcgtccg ttgccgcaag
    27181 tcgctatcga caacgtgaac gtattggaca acgaaggcgc attgaacctg accgaagtac
    27241 ctgccaaact cggcgtaatc ggttccggtg tgattggttt ggaaatgggt tccgtatgga
    27301 accgcgtggg tgcggaagtt accattcttg aagccgcgcc gaccttcctg gctgccgccg
    27361 accaacaaat cgccaaagaa gccttcaaat acttcaccaa agagcaaggt ctgagcatcg
    27421 aattgggtgt gaaaatcggc gacatcaagt ctgaaggcaa aggtgtttcc gttgcttacg
    27481 aaactgctgc cggcgaagcc aaaaccgaag tattcgacaa actgatcgtt gccatcggcc
    27541 gtattccaaa caccaaaggc ctgaacgctg aagccgtagg cttggaaaaa gacgagcgcg
    27601 gctttatcaa agtagatggc gaatgccgta ccaacctgcc taacgtatgg gcaatcggcg
    27661 acgtggttcg cggcccgatg ttggcacaca aagccagcga cgaaggcgtt gccgttgccg
    27721 agcgcattgc cggtcaaaaa ccgcatatcg acttcaacaa cgtaccgttt gtgatttaca
    27781 ccgaccctga aatcgcttgg gtgggtaaaa ccgaagagca gctcaaagcc gaaggcgtgg
    27841 agtacaaaaa aggtacttca ggtttcggtg caaacggccg tgcattggca atgggcaaag
    27901 ccaaaggtac ggttaaagtg ttggcagatg ccaaaaccga ccgtatcttg ggcgtacaca
    27961 tgatcggccc ggttgtcagc gaattggtta ccgaaggcgt gactgcgctc gaattcttcg
    28021 ccagcagcga agatatcgcc cgtattatcc atgcccaccc aaccttgtcc gaagtggttc
    28081 acgaagctgc attggcggtc gacaaacgcg ctttgcacgg ttgatagaca ttaaggccgt
    28141 ctgaaatttt cagacggcct taaggcctcc ggcaaattga atgttccgag agcttcgttt
    28201 tctgatttat aattccgtca gacaaacagc atttacattc attatgaaca aagaaatagt
    28261 cggtattttc tttataccga tgggcatcat cagcatgtgt atggccgcat tgtggcagat
    28321 ttatgtgatg atgaccgaaa cttatacgct caaccgtttc aaagataaag aattggtttg
    28381 gcgcgtggca ttgttgttta tcagtttcag ccttgccgtt tatctgctct gtccgaattc
    28441 gcgtaaaaaa ggcatcgtct tttttattct cgggggaggc ggtgcagtca tgtatctgct
    28501 ggcgcggatg tggttgccct tcagtaaata gtaggaccgt ctgaaaatat gggattggcc
    28561 gcagggcgat tcctaaaacc cactcacctt aaggagaaat ccatgaattt acacgagtat
    28621 caggctaaag aactgctggc tagctacggt ttgcccgtac aaggcggtat tttggcgcac
    28681 aacggcgaag aagccgctgc agcttacgac aaattgggcg gcaaattcgc tgttgtcaaa
    28741 gcacaggtac acgccggcgg ccgcggtaaa gcgggcggcg taaaagtcgt taacagccgc
    28801 gaaaaagcca aagaagtggc tgaaagcctg attggcacca acttggtaac ttaccaaacc
    28861 gatgccaacg gccaacctgt caacagcgtt ttggtttgcg aagacatgta ccctgttcaa
    28921 accgagctgt acttgggcgc agtggttgac cgttctaccc gccgcgttac attcatggct
    28981 tctaccgaag gcggcgtgga aatcgaaaaa gttgctgctg aaactccaga aaaaatcttc
    29041 aaagtaaccg ttgatccgct ggtcggcctg caaccttgcc aagctcgcga agttgccttc
    29101 caactgggct tgaaagacaa acaaatcaac gagttcgcca aactgatgac cggcgcgtac
    29161 aaagcgtttg tcgaaaacga cttcgccctg tttgaagtca acccgctggc agttcgcgaa
    29221 aacggcgcac tggcttgcgt ggacggcaaa atcggcatcg acagcaacgc gctctaccgc
    29281 ctgccaaaaa tcgctgaatt gcgcgacaaa tctcaagaaa acgaacgtga actgaaagct
    29341 tctgaattcg acctgaacta tgttgccctg gaaggtaata tcggctgtat ggttaacggt
    29401 gccggtttgg cgatggccac catggacatc atcaaactca aaggcggcca acctgccaac
    29461 ttcttggacg ttggcggcgg cgcaaccaaa gaccgcgtgg ttgaagcgtt caaactgatt
    29521 ttggaagaca aatccgttaa aggcgtattg atcaacatct tcggcggtat cgtacgttgc
    29581 gacatgattg cggaagccat cgtggcagcc gttaaagaaa tcaacgtcaa cgttcctgtc
    29641 gttgttcgtt tggaaggcaa caacgccgaa ctcggcgcga aaatcctgaa cgaatcaggt
    29701 ctgaaactga cttctgcaga cggtctgaat gacgcagccg aaaaaattgt tgcagccgta
    29761 aacgcctaag gagaaaagaa tgagcgtatt gattaataaa gacactaaag tattggttca
    29821 aggtttcacc ggtaaaaacg gtactttcca ctccgaacaa gctctggctt acggcactaa
    29881 agttgtcggc ggcgttaccc caggcaaagg cggtcaaacc cacctagacc tgcctgtatt
    29941 caacaccatg aaagaagccg ttaaagaaac cggcgctgac gcatccgtga tttacgttcc
    30001 cgctccgttt gtgttggatt ctatcgttga agccgttgat tcaggcgtag gcttggtcgt
    30061 tgtgattacc gaaggcgttc caactctgga catgctcaaa gccaaacgtt acttggaaac
    30121 caacggcaac ggtacccgct tggtcggccc taactgcccg ggcgtgatta ctccgggcga
    30181 gtgcaaaatc ggcattatgc cgggccacat ccacactccg ggccgcatcg gtattatttc
    30241 ccgttccggt acattgactt acgaagccgt ggcacaaacc accaacctgg gcttgggtca
    30301 atcaacctgt atcggtatcg gcggcgaccc gattcctggt atgaaccaaa tcgacgcact
    30361 gaaactcttc caagaagacc cagataccga tgccatcatc atgattggtg aaatcggtgg
    30421 tactgcggaa gaagaagcag ccgaatacat ccaatccaac gtaaccaaac ctgttgtcgg
    30481 ttacatcgcc ggtgttaccg ctcctaaagg caaacgcatg ggccacgccg gtgcgattat
    30541 ctccggcggt aaaggtactg ccgaagaaaa attcgccgcg tttgaaaaag ccggtatcgc
    30601 ctacacccgc agccctgccg agttgggcac gacgatgctg gaagtgttga aagcaaaagg
    30661 tttggcataa tcaggtttga caactgattg aacatcaaat gccgtctgaa accggaaatc
    30721 gggtttcaga cggcattttg tttgtcattt caaaaagagg cagcctcaac atactcacat
    30781 tatttttgcc ctttgaggca gtcagaatat agtggattaa aaacaaaata gtacaacact
    30841 cgacgttgaa ggtttaacca tggcatactc tgcggactta agaaacaaag ctttaaacca
    30901 tagcggatta acaaaaatca ggacaaggcg gcgggccgca gacggtacaa atggtacgga
    30961 accgatccgc ccggtgctcc atcaccttag ggaatcgttc cctttgggcc ggggcggggc
    31021 aacgccgtac cggtttttgt taattcgcta tacagcaggc gttggtggaa atcccgaata
    31081 agcggttcaa aacgtgtcgc tgccgcggca gccatataca gcgccttacg caccgcagac
    31141 cttccgccga agcagcggct tttgaatttg gtttccccgc tctccctcgg gtgcggggca
    31201 atgccgacca aacccgctat ccgtttgtgc gacagccgcc ccaattcggg cagcatcgcc
    31261 atcagcgtag ccgtcgttat cgaaccgatg cctttgattt gctccgccac ttgggctttg
    31321 ccgtcaaaat gcgtgcgggt gtggttgtcg atttgtttgt ccgattcgtc aatcagccgg
    31381 tcaaaacggg caatcagttg tttgacgctt tcgacttgcg tttcgtgaac ctgatgcaga
    31441 cattttgtaa tagctgccat tacgcctact aggatatata ccttctatat atag
//
LOCUS       DAXFZR010000014        29004 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000014 DAXFZR010000000
VERSION     DAXFZR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29004
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.014"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            413..780
                     /locus_tag="ACFJAS_000542"
                     /pseudo
     CDS             413..780
                     /locus_tag="ACFJAS_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            777..1088
                     /locus_tag="ACFJAS_000543"
     CDS             777..1088
                     /locus_tag="ACFJAS_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3981870.1"
                     /translation="MKYKSGIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPGGAALKLLTIVKNKGIEAI
                     A"
     gene            1199..1411
                     /locus_tag="ACFJAS_000544"
     CDS             1199..1411
                     /locus_tag="ACFJAS_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981871.1"
                     /translation="MPDRIKPSRNGGGTDGIARQQGTAQTGAKPETSPTARHGDFLRP
                     LQGGIGFKRRGLEGKTGGAQNCLNAV"
     gene            complement(1861..2416)
                     /locus_tag="ACFJAS_000545"
                     /pseudo
     CDS             complement(1861..2416)
                     /locus_tag="ACFJAS_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2413..3220)
                     /locus_tag="ACFJAS_000546"
                     /pseudo
     CDS             complement(2413..3220)
                     /locus_tag="ACFJAS_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(3213..3383)
                     /locus_tag="ACFJAS_000547"
                     /pseudo
     CDS             complement(3213..3383)
                     /locus_tag="ACFJAS_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(3350..4141)
                     /locus_tag="ACFJAS_000548"
     CDS             complement(3350..4141)
                     /locus_tag="ACFJAS_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC3981872.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(4370..7000)
                     /gene="leuS"
                     /locus_tag="ACFJAS_000549"
     CDS             complement(4370..7000)
                     /gene="leuS"
                     /locus_tag="ACFJAS_000549"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC3981873.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVSDNAFDANRWQEWYGNKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            7267..7783
                     /locus_tag="ACFJAS_000550"
                     /pseudo
     CDS             7267..7783
                     /locus_tag="ACFJAS_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            7790..8233
                     /locus_tag="ACFJAS_000551"
     CDS             7790..8233
                     /locus_tag="ACFJAS_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC3981874.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            8402..10459
                     /gene="ppk1"
                     /locus_tag="ACFJAS_000552"
     CDS             8402..10459
                     /gene="ppk1"
                     /locus_tag="ACFJAS_000552"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC3981875.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKTPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(10608..11711)
                     /gene="dnaN"
                     /locus_tag="ACFJAS_000553"
     CDS             complement(10608..11711)
                     /gene="dnaN"
                     /locus_tag="ACFJAS_000553"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC3981876.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(11791..13347)
                     /gene="dnaA"
                     /locus_tag="ACFJAS_000554"
     CDS             complement(11791..13347)
                     /gene="dnaA"
                     /locus_tag="ACFJAS_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC3981877.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            13639..13773
                     /gene="rpmH"
                     /locus_tag="ACFJAS_000555"
     CDS             13639..13773
                     /gene="rpmH"
                     /locus_tag="ACFJAS_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC3981878.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            13776..14141
                     /gene="rnpA"
                     /locus_tag="ACFJAS_000556"
     CDS             13776..14141
                     /gene="rnpA"
                     /locus_tag="ACFJAS_000556"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC3981879.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            14206..14427
                     /gene="yidD"
                     /locus_tag="ACFJAS_000557"
     CDS             14206..14427
                     /gene="yidD"
                     /locus_tag="ACFJAS_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC3981880.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPVP"
     gene            14600..16237
                     /gene="yidC"
                     /locus_tag="ACFJAS_000558"
     CDS             14600..16237
                     /gene="yidC"
                     /locus_tag="ACFJAS_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC3981881.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(16318..17028)
                     /locus_tag="ACFJAS_000559"
     CDS             complement(16318..17028)
                     /locus_tag="ACFJAS_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC3981882.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(17090..17680)
                     /locus_tag="ACFJAS_000560"
     CDS             complement(17090..17680)
                     /locus_tag="ACFJAS_000560"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC3981883.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            17828..18328
                     /locus_tag="ACFJAS_000561"
     CDS             17828..18328
                     /locus_tag="ACFJAS_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC3981884.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIILFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            18362..18541
                     /gene="rpmF"
                     /locus_tag="ACFJAS_000562"
     CDS             18362..18541
                     /gene="rpmF"
                     /locus_tag="ACFJAS_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC3981885.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            18716..19243
                     /locus_tag="ACFJAS_000563"
     CDS             18716..19243
                     /locus_tag="ACFJAS_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC3981886.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDSEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            19333..20379
                     /gene="plsX"
                     /locus_tag="ACFJAS_000564"
     CDS             19333..20379
                     /gene="plsX"
                     /locus_tag="ACFJAS_000564"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC3981887.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            20517..20768
                     /locus_tag="ACFJAS_000565"
     CDS             20517..20768
                     /locus_tag="ACFJAS_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC3981888.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            20795..21013
                     /locus_tag="ACFJAS_000566"
                     /pseudo
     CDS             20795..21013
                     /locus_tag="ACFJAS_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21164..21355
                     /locus_tag="ACFJAS_000567"
                     /pseudo
     CDS             21164..21355
                     /locus_tag="ACFJAS_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21385..21588
                     /locus_tag="ACFJAS_000568"
     CDS             21385..21588
                     /locus_tag="ACFJAS_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981889.1"
                     /translation="MPSEAAWAFRRHFLHLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            21615..22577
                     /locus_tag="ACFJAS_000569"
     CDS             21615..22577
                     /locus_tag="ACFJAS_000569"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC3981890.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            22624..22995
                     /locus_tag="ACFJAS_000570"
     CDS             22624..22995
                     /locus_tag="ACFJAS_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC3981891.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            23021..23947
                     /gene="fabD"
                     /locus_tag="ACFJAS_000571"
     CDS             23021..23947
                     /gene="fabD"
                     /locus_tag="ACFJAS_000571"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC3981892.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            24086..25954
                     /gene="msbA"
                     /locus_tag="ACFJAS_000572"
     CDS             24086..25954
                     /gene="msbA"
                     /locus_tag="ACFJAS_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC3981893.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPDLSAAAGILSTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            26054..27619
                     /gene="guaA"
                     /locus_tag="ACFJAS_000573"
     CDS             26054..27619
                     /gene="guaA"
                     /locus_tag="ACFJAS_000573"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC3981894.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYYYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            27681..28427
                     /gene="fabG"
                     /locus_tag="ACFJAS_000574"
     CDS             27681..28427
                     /gene="fabG"
                     /locus_tag="ACFJAS_000574"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC3981895.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            28492..28977
                     /locus_tag="ACFJAS_000575"
     CDS             28492..28977
                     /locus_tag="ACFJAS_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC3981896.1"
                     /translation="MNTHHHLPCPCGSGTSYAGCCRPLHLRQILPPTAEALMRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPTVPLPTMKQPCICGSSKKFKACCGKYLQPV
                     A"
BASE COUNT         7127 a         8425 c         7117 g         6335 t
ORIGIN      
        1 ccgtaccggt ttaaatttaa ttcactataa aaataagaaa aagataaaaa attggtaaca
       61 aatgcggtaa caaatggtaa cgaatcggta acaacttttg gggttttccg gttttttacc
      121 gtcttggcag tgggagcgta gcggaatgaa aagccaaaac gcacggaacc gcgcctattt
      181 tgagcaggaa tggcggttaa accggttggt tatattgcgg ggcataggaa gacagcgaaa
      241 cgcgctaaaa acgcaatttg agccattaaa agcgattgat taaaaaataa tcaaattagc
      301 cgccggtgtt tcaggcggca taaacggaga aattgcaggg tataaaaagg cggcttgccg
      361 tgttgcctgt ctttggtata atccccagta tcactaagta aacggttgca caatgcggat
      421 attcaaaaac caatggatag tgaaatttgc caagaagcac aaaatcagtg attcagagct
      481 gatagaggcg gtagagcggg cagataacgg gctgatagat gcagatttgg gcggcgcgtg
      541 attaagcagc gtatagcaag gcaagggcaa ggcagaagcg gcggttatcg cagtctgata
      601 tttttcaggc atggagaacg ggcgtttttc atgaccgcct ttgccaagaa cgaccgcgaa
      661 aacatcactg ataaagaact tacagaatta aagaaaaccg ccgccatcat cttggcaatg
      721 acagaaaccg acatagagca agccaagtca aacggcactt tcacggagat acagccatga
      781 aatataaaag cgggattttc gcagcaatac acgaaaccat gcagggattg cacgacatcg
      841 gcgcaatcga caaaaagact atgcgcggct ttgacaagtc ctgcctgacc gaaatcaagc
      901 cgttgagcgg cggggacatc aaggcaatca gggagaagga ggcactatcg caagccgctt
      961 tcgccatcta tctcaacgtg gggaaaaatc acgtttcggc ttgggagcgg ggcgttaaaa
     1021 agcccggcgg cgcagcgttg aagctgctga ccatcgtcaa aaacaagggc atcgaagcca
     1081 ttgcgtagcc gacttggcaa acggcaaaat cagcaagttc acatatagac gcgctgctga
     1141 atatgcctgc caagacaggc aagaccgccg aactgaatat cagggcgtag ccgcacaaat
     1201 gcccgaccgc atcaaaccaa gccgaaacgg cggcggtaca gacggcatag cccgacagca
     1261 aggcacggcg cagacgggcg cgaaacccga aacatccccg accgcgaggc acggggattt
     1321 tttgcgcccg ttgcaggggg ggattggatt taagcggcgc gggcttgaag gcaaaacggg
     1381 cggggcgcag aactgtttaa atgccgtctg aatctcaaac gatttcagac ggcattttga
     1441 aacaacggct caaattctcg atccccttcc cttaacgccg acgtttttta ttaacgcgcc
     1501 ccttatttct gacactttgc tcataaaccg gcataacggt cggcaacaac cgttttagat
     1561 tttctatacg ggcattgttt acagcatcag cataagacac cgtactctgg aataccgcct
     1621 aaaccctgcg gatagttttg gatgtggtat cgacattgtc ggcagacttg tctaactcga
     1681 ccgttttcaa ataatctttg gcagatgctg cattcagtgt cgcggaatcc gcgtattttg
     1741 aacaaaaggt cgggctactt tatcacaata gcagaaagat ggaaaacggc ggggattggt
     1801 gaaaggtcgt ctgaaatgat attcagacga ccttgaacgg ttgtgtttta aatatggtat
     1861 ttattcattt gtttcaccat caggcagtcc aaaccacctt aagggaatcg tgttaaattg
     1921 gtaatggcag ttaacactga taatataatc taaaacatct ttaatattcc ggttttgtaa
     1981 accaatcatt caaaacatag acatatccaa cccgccaacc taaagagtga actaactttg
     2041 tatattgttt tctcttaaaa tcgcaagttt gtaatttctc atctactgaa ccgggagttt
     2101 gttggaattt gatctcaata ataaataagg tatcacggat gatgataaat aagccattat
     2161 cgggcagtaa tcttttagac agatggtctt tccaattaat attgtattcc agtctttcca
     2221 aaaatcggta aaactcatat tttttgaaac aataagcctt caattctcca ttaaaccaaa
     2281 cttcataacc cgcattatca tctgtttttc ttaagtcgta cccgggaatt tcttcaaaca
     2341 actttcgtaa gtctgtacgt tcttcgaatc tgagtcctgt ttgtgtcctt gcaccgccga
     2401 ttccgccaat aatcattttt ctaacctttt ctttgctaat tcaaaaaatc ttcttccagt
     2461 tcgcaaccgc aaaatctccg accatgtttt aaggcggcaa cgcctgttgt gccgctgccc
     2521 ataaaagggt caaaaattaa atctccgata tttgaagccg atagtatgca gcgttcaagt
     2581 aaggagagtg gtttttgtgt cggatgtttg ccgaatgttt tttcggtttt atttggtgga
     2641 ggaaatgtcc aaacacattt catctgtttg ccattatttt gtgctttcat catttcataa
     2701 tgaaacgtat gtttggcttt tttatttttc tttgcccata agattgtttc tgtcgaatgg
     2761 gtaaagaaac ggcaggacaa attaggggga ggattgggtt tttcccaagt aatattgttc
     2821 aaaatatggt agccgacggt ttgcatcagg tagccgatta aatagatatt atgaaatgtg
     2881 ccgcaaaccc aaattgtgcc gtttggtttt aataaggcgt aacacagtcg gagccattct
     2941 tcgtaaaatt ccaaatctgc cgccattcct ttggatttat cccagttgcc cttattgacg
     3001 gaaaccattt gcccgttttg acaactgaaa ccgtcattgg aaagaaagta aggcgggtct
     3061 gcgaaaatca tatcaaaaca gccgtttgga tgtttttcca atatcttccg catgacgtta
     3121 agggaatttt cgttgtagag gacaaggtcg tctgaaacga ttttttcaaa cgactttgat
     3181 aaatggcagc ttcttcagcc ggtttgtcaa aatcaatcat aaattgccaa taattctccg
     3241 acttttttac gactgctgcc ctttgcggca atattgcgtt gtacttcaac cgtttttaga
     3301 atggcagaag aataaatatt tctcgtaaat tcagtatcat gatttgaaat caaaacgcct
     3361 tgggagttgg gtgcaatttg tcgatttttt tgtaaagtcc gcgaccaatg aattcgatca
     3421 tattttgatc acgcaaaatt tgcaactgtt ggcggatttt gtctttgata tggttgtttt
     3481 ggggaaattg gatggatagt ttgttttcaa attcatacat ttgcgacaat gtgaattctt
     3541 cggtgagttg gtcgatacat ttcataacag ccaaaagcca gtctttgcgc tccgcatttt
     3601 ggttgcgtaa aaacaggttg gattgccatt ttttcagaac ggtttcgggt tcgataatgc
     3661 gggaatcgtc tattaagaat atttttccgc tttcaggcaa aggggcgaga ttgatggaac
     3721 acataatgtg gtgcggtcgg tttttaatgc ctttatttct gggaataatc atatccggcg
     3781 tgatgaaatg tttgggtaca agcaccaatt gccgtatgga gtaatccgct tttttatatg
     3841 caagaaagaa aaagttgggg ttggtatctg accggatgcg ctccaacata gtgcgatatg
     3901 caccgtcagg cacgctgttg cctatggttt tttgattttt actctttaat tcatattgct
     3961 cgtggcaatt tgggcaaaag aggtctgcaa caggtttgtt attggcaaat ctctgcatcg
     4021 gcttgcttcc gcaacagggg caatagccgt ttttttccag ccaagcctcg ctcattacac
     4081 ggattttgtg ggttgcttta ttttgttgct ttcccaattg ggtatcgaaa aataaattca
     4141 tattttggat tttgagatta atttttagta gattaacaaa aatcaggaca aggagacgaa
     4201 gccgcagaca gcagggcaag gcggggcaac gccgtaccgg tttaaagtta atccgctata
     4261 tttgaggtct gtcttgcaga caacccaatc tgccttaaaa aggccgtctg aaaccctgtt
     4321 tccaagtttc agacggcctt tatccgtatg gctaaacctt aaaagcggtt tagacgacga
     4381 tgttcaccag tctgcccggt acgacgatga ttttcttcgc aggcttgcct tccatgaatt
     4441 tcactgcgcc ttcgttggcg agtgcggcgg cttcgaggtc ggctttggag gcgtcggcgg
     4501 cgacggtgat tttgccgcgc agtttgccgt tgacttgaac catcacttcg atttcggatt
     4561 tgaccagggc ggcttcgtcg actgtcggcc agcctgcttc ccacagtttc gcgccgttca
     4621 attcgctcca cagggtttcg cagatgtgcg gcacgatggg ccacaacagg cgtacggcgg
     4681 cttccaatac ttcttgggcg acggcgcggc cttgttcgct gccggtgtcg gttttgtcgt
     4741 attggttgag cagttccatc acggcggcga tggcggtgtt gaactgctgg cggcggccgt
     4801 agtcgtcgct gactttggcg gtggtggaat gcagtttgtg gcgcaggtct ttgagttctt
     4861 tagacaaacc gtcttggttg cctgcaaatg ctttgaccgc gccgccttgc ttcaggtatt
     4921 cgtaaacggt acgccacaga cggcgcagga agcggtgtgc accttcgacg ccgctgtcgc
     4981 tccattcgag ggactgttcg ggcggtgcgg cgaacatcat gaacaggcgg gcggtgtccg
     5041 cgccgtaggc gttaatcagt tcttgcggat cgacgccgtt gtttttggat ttggacattt
     5101 tttccgtgcc gctgatgacg acgggcagtc cgtcggcttt gaggacggcg gaaacggggc
     5161 ggcctttgtc atcgaaagtc agctcgacat cggcggggtt gatccagtct ttgccgcctt
     5221 tgtcgttttc gcggtagtag gtttcgcaga cgaccatacc ttgcgtgagc aggcgttcaa
     5281 acggttcgtc aacattgacc aaaccttcgt cgcgcatcag tttggtgaag aagcgcgcgt
     5341 acaggaggtg caaaatcgcg tgttcgatgc cgccgatgta ttggtcgacc gcgccccagt
     5401 atttcgcgga ttctgccgat accatgccgt ctgaaaactt gggcgacata tagcggaaga
     5461 agtaccagct cgactccatg aaggtgtcca tggtgtcggt ttcgcgtttc gccgcgccgc
     5521 cgcagcacgg gcaggaagtt tcgtaaaact cgggcatttt tgccagcggc gaacccatac
     5581 cgtcgggtac gacgttttca ggcaggacga cgggcagttg gtcggcaggg acgggaacgt
     5641 ctccgcattt ttcgcaatgg acgatgggaa tcgggcagcc ccagtagcgt tggcgcgaaa
     5701 tgccccagtc gcgcaggcgg tattgggttt tcggttcgcc cgcaccttgg ctttgcagct
     5761 tggcggcaac ggcatcaaat gccgtctgaa aatccaagcc gtccaagtcg ccgctgttga
     5821 ccaatacgcc gttttctttg ttgccgtacc attcttgcca tcggtttgcg tcgaatgcgt
     5881 tgtcgctgac ggcaatgact tgttttttcg gcagattgta tttggcggca aactcgaaat
     5941 cgcgttcgtc gtgcgccgga acagccatca ccgcgccgtc gccgtagccc cacaagacat
     6001 agttggcaat ccacacttcc agcttgtcgc cgttgagcgg gttgacgacg tagcggccgg
     6061 tcggcacgcc ttttttctcc atcgtcgcca tatcggcttc ggcaaccgaa ccggctttgc
     6121 attcggcgat aaatgcctgc aattcgggtt tgtcggcggc tgcggctgtt gccagcggat
     6181 gttcggcggc aacagcgaca taagtcgcac ccatcagcgt gtcggggcgg gtggtataaa
     6241 cttgcaggaa tttcgcgtaa tcgccttcca aaccttgctt gctgtcgtct gaaacggcga
     6301 agcgcacggt catgccgcga gatttgccga tccagttgcg ctgcatggtt ttgacttgtt
     6361 ccggccagtg ttccagcttg tccaaatcgt tgagcagctc ttcggcgtaa tccgtgattt
     6421 tgaagtaata catcgggatt tcgcgttttt cgatcaacgc gcccgaacgc cagccgcgtc
     6481 cgtcgatgac ttgctcgttg gcaaggacgg tttggtcgac cgggtcccag ttcaccgtgc
     6541 cgtttttgcg atagacgatg cctttttcaa acagcttggt aaacagccat tgttcccagc
     6601 ggtagtattc gggtttgcag gtggcgactt cgcgttccca gtcaaccgca aaacccaggc
     6661 ttttgagctg ggttttcatg tattcgatgt tgtcgtaggt ccaagcggcg ggggcgacgt
     6721 tgtttttcat cgccgcattt tccgccggca tgccgaacgc gtcccaaccc ataggctgca
     6781 taacgttgaa gccgtttaaa agtttgaagc ggctcaatac gtcgccgatg gtgtagttgc
     6841 gtacatgccc catatgcagc ttgccgctag ggtaagggaa catcgaaagg caataatatt
     6901 tgggtttgga agcgtcttcg gagacgttgg aaatacgggc gtcgtcccat tttttctgcg
     6961 ccgcaggctc gatggcggcg ggctggtaat gttcttgcat ggtcattttg tttttcgctt
     7021 gaaaacgtta ggaatttaaa ggaggaatta taacaggttg ccgatgtcgg cgaatcggca
     7081 aattgccggc aggatgcgta aattcgcacg cgcatcattc cgtatgccgt acaaatacac
     7141 cgcgtttact gatacgcacg ttttttatgc taatattacg aaccaaaatc aaacgtttaa
     7201 aactctcctg atgcggctct tccgaacaaa aggcagacgg gcatcgggta aaaaaggatt
     7261 ctgcatatga aaatcaaaca aatcgtcaaa ccgagcttgg cagtattggc ggcaggcatt
     7321 ctgtctgcct gcgcaaccat aaagcaacgt caaagccgac ggcacgaccg acaatccggt
     7381 tttcccggaa ccctattccg taacgctcga caacaagcgc ggcacattcc cgacttatga
     7441 cgaactggat cagatgcgcc ccggtctgac caaagacgac atctacaaaa tcccgggccg
     7501 cccgcattac gacgaaggta tgtacggcgt gcgcgaatgg gattacctgt tccacttcca
     7561 caccccgggc gtgggtatcg accctgaaaa cacttccggc gtggaaggcg ttactacctg
     7621 ccaatacaaa gtgattttcg ataaagacaa atttgcccgc agcttctact ggaaccccgt
     7681 cttcccgaaa gatgccgtct gcccgccgcc cgcacccaaa gccgagccgc aagtcatcat
     7741 ccgcgaaatc gtgccggcaa aaccgaaacg tatccgccaa taagccgcta tgccgccccg
     7801 cctgctttca gggatattgt gctgcctgct gacgactgcc cccgtattcg cacaggggca
     7861 gccggatgcg gtcagtgcct atattcaaaa gaaaaaagtc atcgtcgaca catccaaggc
     7921 ggaactctgt tttgccgacg accgtcagtg ccaccccgtc ctcatcggca ctgccacacc
     7981 caaggggacg ttcggactga cgctgaacag taccgacaag cccggttacg gcggcgaagt
     8041 catcggtttc aagcaggagg gcgattttct tttcgccctg caccgcgttt ggaatcagat
     8101 accgtccgaa aggcgaaacg aacgcatcgc ctccccgtcc gtgtccgaca ggattatgac
     8161 caacggctgc atcaacgtca gcgatgcggt gtacgaaaaa ctgcgtcatt attttgtgtt
     8221 ggaagtgatt tgaaacagac ggataccgca cgtgccggta tccgttttca cattgccccg
     8281 atgcctgaaa cagaccgtcc gtcacgtcat gccgcctgaa accggcgcag atgccgccaa
     8341 gccttcagac ggcattgtcc gcccgctccg accgaacaac aaccatcttt gggagaacct
     8401 tatgaccgaa caaaaccgca tcctctgccg cgaactgagc ctgctggcat tcaaccgccg
     8461 cgtgttggca caggcggaag acaaaaacgt cccccttttg gaacgcctgc gcttcctgtg
     8521 catcgtgtcg tccaatcttg acgagttttt tgaagtccgc atggcgtggc tcaagcgtga
     8581 aaacaagctg cacccccggc gcaggccgga caacggcaaa acgccgtctg aaaccatcgc
     8641 cgacgttacc gaagcggcgc gctccctgat acggcaccag tacgacctgt tcaacaacgt
     8701 ccttcagccc gagttggcgc gggaaagcat ccatttttac cgccgccgca attggacggg
     8761 cacgcagaaa aaatggatcg aagactattt cgaccgcgaa ctgctgccga tcctgacccc
     8821 catcggactc gacccttccc accctttccc gcgcccgctg aacaaatcgc tcaacttcgc
     8881 cgtagaactc gacggcacgg acgcgttcgg caggccttcg ggtatggcga ttgtccaagc
     8941 accgcgcatc ctgccgcgcg ttgttcccct gccgtccgaa ctgtgtggcg gcggacacgg
     9001 cttcgtcttc ctgtcgtcca tcctccacgc ccacgtcggc aaactcttcc cgggcatgaa
     9061 cgtcaaaggc tgccaccagt tccgcctcac gcgcgacagc gacttgaccg ttgacgaaga
     9121 agacgtacaa aacctccgcg ccgccattca aaacgaactg cacgaccgcg aatacggcga
     9181 cggcgtgcgg ctcgaagtcg ccgacacctg tcccgcctac atccgcgact ttctgctcgc
     9241 gcaattcagg ctgaccgacg ccgaactcta tcaggtcaaa ggcccggtca acctcgtccg
     9301 cctcaacgcc gtccccgacc tagtcaaccg ccccgatttg aaatttccgc cgcacacgcc
     9361 gggccgtctg aaagccttgg gcaaaaacag cccgatcttc gatttggtgc gccaatcgcc
     9421 catcctgctg caccacccct accaatcttt cgatcccgtg gtcgatatga tacgcgaagc
     9481 cgccgccgat cccgccgttc ttgccgtcaa aatgacgatt taccgcaccg gcacgcgctc
     9541 cgaactcgtc cccgccctga tgaaggcggc actcgccggc aaacaagtaa ccgtcgtcgt
     9601 cgaactgatg gcgcgttttg acgaagccaa caacgtcaac tgggcgaagc agctcgaaga
     9661 ggcgggagcg cacgtcgtgt acggcgtgtt cggctacaaa gtccacgcca aaatggcact
     9721 ggtcatccgc cgcgaagacg gcgtgctcaa gcgttacgcc cacctcggca cgggcaacta
     9781 ccaccaaggc acatcgcgca tctacaccga cttcggcctc attaccgccg acgaacaaat
     9841 caccgccgac gtgaacacat tatttatgga aatcacaggc ttgggcaaac ccgggcggct
     9901 gaacaaactc taccaaagtc cgtttaccct gcacaaaatg gttatcggcc gcatcgctcg
     9961 cgaaaccgaa cacgcaaaag ccggcaaacc ggcgcggatt accgccaaga tgaattcgct
    10021 catcgaaccg actgtcatag aagccctgta tcgggcaagc gcggcaggcg tgcaaatcga
    10081 tttgattgtg cgcggtatgt gtaccttgcg cccgggtgta aaaggcttgt ccgaaaacat
    10141 ccgcgtccgc tccatcgtcg gcaggcagct cgaacacgcg cgcgtatatt gtttccacaa
    10201 caacggcgcg gacgacacct ttatttccag cgcggattgg atggggcgca atttcttccg
    10261 ccgcatcgaa accgccaccc cgattaccgc gcccgaactc aaaaaacgcg tcatccgcga
    10321 aggtttggaa atggcactgg cagacaatac ccacgcgtgg ctgatgcagc ccgacggcgg
    10381 ctatatccgc gccgcgcccg ccgaaggcga aagcgaagcc gacctgcaaa acgatttatg
    10441 ggatttgctc cgaggctgac ccgcacaagc acttccccca tacgctccca accccaatcc
    10501 gccgcattcc agacggcatt gcctttccga cagccgaaca aaaccccgaa tccgtctgca
    10561 cggttcgggg catatctcca atacgggcat cgtgttccgg aaaaccgtca aatccgcatc
    10621 ggcatcacaa tatatttgaa attcggattg ttcggcacgg taaacagcgt cgagcggttg
    10681 gcgtcgccga aggcaagctg catatcgtcg gaatggatgt tgcgcaacac gtccatcaaa
    10741 tagccgatat tgaaaccgac ttcgagttcg ccgccctgat aggcgatttc gatttcttcg
    10801 cgcgcttctt cctgctcgtt gttgctgcac acgacgctca acaggccggg ttgcaggaac
    10861 aggcgcgcgc cacggaattt ttcgttggca agaatggaca cacgttccag cgcgcccaaa
    10921 agttcggcac gggacaacac gaaaatcttg tcgttgtcca aaggaatcac gcggttgaaa
    10981 tcggggaatt tgccgtcgat gaccttgctg acgatggtcg tgccgttgca ttggaaacgc
    11041 acctgcttgt ccagcagctc gatttgaatc ggatcgtcgg ggttgttcaa cagtttgaac
    11101 agttccagca ccgttttgcg cggcaaaatc acttcggcgc gcggcaaatc cgcatcaatc
    11161 gcgcaggctg cataggcaag gcggtgtccg tcggtcgcca caaggcgcaa ctggctgccc
    11221 tcaacctgca tcagcagacc gttgagataa tagcggatgt cctgcaccgc catgctgtac
    11281 tgcacttgcg acagcatggt tttgaaacgc tcctgcccca gcgagaaagt cgcgctgatg
    11341 tcctcgccga cattcatcat cggaaaatcg gcggcaggca gggtttgcag ggcaaaacgc
    11401 gatttgcccg ccttcagcgt cagacggttg tcgtcccaat ccagcgacac cagcgcaccg
    11461 gcaggcagcg cgcgcaaaat gtcctgaaat ttcttggcat tggtagtgat gcggaagtcg
    11521 cccgcacctc cctcgggacc cgcggtgtcg atttggattt ccaaatcggt tgccaagagt
    11581 ttggtctgac cgcccctgcc ctcaatcagc acattggaca ggatgggcag ggtgtgtcgg
    11641 cgttcgacga tgccggtaac ggcttgcagc ggcttgagca ggctgtcgcg ctcggcttgt
    11701 aaaatcaaca ttttcgctcc ttaaatcggt tggatttcct acccttcgga cggcatggtc
    11761 aatgtcatgc cgtctgaaag gcgtgtccgg tcagttttga atcagaatca gcagtttctc
    11821 gtaatcctgc gccaactcgg ggtcttcctc gcgcagtttc gccaccgccc tgatgccgtg
    11881 catgacggtc gtatggtcgc gtccgccgaa cgaatcgccg atagacggca ggctcaaagt
    11941 ggtcaattct ttggtcaggc tcatggcaac ctgacgcgga cgggcaatgt tgcgcgtgcg
    12001 ttttttgccg agtacgtcgc tgattttgat gcggtaatat ttcgccaccg catcgatgat
    12061 gatgtcggcg gtgatgactt tgtgcttctc ggcaataatg tcctgcaaag cggtacgcgc
    12121 caaatcgatg tcgatgacgg gacggttcat aaagcggctg ctcgctccga cacggttgaa
    12181 cgcgccttcc agttcgcgca cgttggaacg gatcagattg gcaatgaaca gcgcggcttc
    12241 gtcttcgata ctgatgcccg ccgcttccgc ctttttctgc aaaatggcga tacgcatttc
    12301 caattcgggc ggctcgagtt ccaaagtcag cccccacgaa aagcgggatt tgaggcggtc
    12361 gtccatacct tcgattttgg cgggtaaaac atcgcaagtg aggatgagct gttttttctc
    12421 attgtgaaaa tggttgtaca gatagaaaaa ttcttccatc gtacggtctt ttcctttgat
    12481 gaactgaata tcgtcgataa tcagcaggtc gtattgcttg tattgctgct tgaacacgtc
    12541 gtaggtattg ttgcgaaccg ccttcataaa gctgcggatg tagtcgtccg aatgcatata
    12601 gcgcactttg gcatcgggac ggtttttcaa cagctcgttg ccgaccgcct gcacaaggtg
    12661 ggttttgccc aaacccgtgc tgccgtacag gaagaacggg ttgtaactct gccccgggtt
    12721 ttccgcaatc gcctgcgccg cagccgccgc aaggcggttg cccttacctt ctaccaacgt
    12781 atcaaacgtg taatccggag acaggttggt ttgttcgtaa cgcgcttctt ccgcatcacg
    12841 ctgcgcatcc gtccgcgctt tggcaactgc cgccgattcc ggccgggaag caggcccggc
    12901 agcctgacgc ggctcgtgcg gcaggttttt catacgttcc gccaaaatat ccgccgccgt
    12961 tttcgacgca gcgggtttga caggcttttc agacggcagc tcgtccaaca gaacctcctg
    13021 cacgggcatc tcctccgacc ccgcgtgcaa ggacggctcg gcaggttcga cagcaccttc
    13081 aaccgccgcc atctcataac gcacgccttc tcccggtttg aatacgaagg cgggacggcc
    13141 ggcagccaac tcttccctca ccgcttctat ttttccggca aactggctct tgagcatatt
    13201 gcaggcaaac tggttcttgc cgtacaccac ccatacgcca ccctcctcac caaccgtaag
    13261 gggcgcaatc cattgcgcaa actgcccgtg aggcaacata tcgtgaagac ggcggaggca
    13321 cagcggccaa aactctgcta atgtcatgga taggctcgaa tcggtaaaaa tgaaatcgaa
    13381 aacaaagaaa atataatatt ttcaaaaaga aaacaaatct gttgaatacg cgtcggttca
    13441 aaacgcagcg aaccgattat accgactcgc cgatatttta tccacaaccc gtgcaaaaat
    13501 ttatccacag aaaggcggca gaaatccgca ggcaatcggg caatcctcct gcaaagtttc
    13561 tatattgatt gacaaaagcg gcaaattgga gtgtaattca cggtttaatt atctacccat
    13621 tctattttag gaaccatcat gaaacgcact tatcaacctt ccgttaccaa acgcaaacgc
    13681 acccacggct tcctcgtgcg ctccaaaaca cgcggcggac gcgcagtatt ggccgcacgc
    13741 cgtgccaaag gccgcaaacg cctggcagtg taattttgga ctaccgcttc ggaaggcagt
    13801 accgcttgtt aaaaacggat gatttttcat ccgtttttgc attcagaaac cgccgcagcc
    13861 gcgacctgct gcaagtttcg cgctcaaacg gcaacgggct ggaccatccc cgcatcggtc
    13921 tggtggtcgg caaaaaaacc gccaaacgcg ccaacgagcg gaactatatg aaacgcgtca
    13981 tccgcgactg gttcagattg aacaaaaacc ggctgccgcc tcaggatttc gtcgtgcgcg
    14041 tccgccggaa attcgacagg gctaccgcaa aacaggcaag ggcggaactg gcacaactca
    14101 tgttcggcaa ccccgcaacc ggatgcggga aacaggtatg atcagaacgg taccctgcag
    14161 gcaaggttca gacggcaacg ggtttcccat acaaggaaca tcccgatgaa cttcctattg
    14221 tccaaactgc tgctggggct gataaggttt taccaatact gcatcagccc gctgattccg
    14281 ccgcgctgcc gttatacgcc gacctgttcg caatacgcgg tcgaagcggt caaaaaatac
    14341 ggcgcattca aaggcctccg gctcgccatc aagcgcattg cacgctgcca ccctttcggc
    14401 ggacacggac acgaccccgt tccctgaccc gacgcaatat tcaaattgca cgctttcctt
    14461 ttatttccca tcggtttcta tataatgccg tctgaacctt cgggctggcg gcacggccgt
    14521 cgggtatgaa gcccgccctt attccccgtc tatcggaaca cgcaacctgc ggcatttccg
    14581 accattcagg aaactctata tggattttaa aagactcacg gcgtttttcg ccatcgcgct
    14641 ggtgattatg atcggctggg aaaaaatgtt ccccaccccg aaacccgtcc ccgcgcccca
    14701 acaggcggca caaaaacagg cagcaaccgc ttccgccgaa gccgcgctcg cgcccgcaac
    14761 gccgattacc gtaacgaccg acacggttca agccgttatt gatgaaaaaa gtggcgacct
    14821 gcgccggctg accctgctca aatacaaagc aaccggcgac gaaaacaaac cgttcgtcct
    14881 gtttggcgac ggcaaagaat acacctacgt cgcccaatcc gaacttttgg acgcgcaggg
    14941 caacaacatt ctgaaaggca tcggctttag cgcaccgaaa aaacagtaca ccctcaacgg
    15001 cgacacagtc gaagtccgcc tgagcgcgcc cgaaaccaac ggactgaaaa tcgacaaagt
    15061 ctataccttt accaaagaca gctatctggt caacgtccgc ttcgacatcg ccaacggcag
    15121 cggtcaaacc gccaacctga gcgcggacta ccgcatcgtc cgcgaccaca gcgaacccga
    15181 gggtcaaggc tactttaccc actcttacgt cggccctgtt gtttataccc ctgaaggcaa
    15241 cttccaaaaa gtcagcttct ccgacttgga cgacgatgcg aaatccggca aatccgaggc
    15301 cgaatacatc cgcaaaaccc cgaccggttg gctcggcatg attgaacacc acttcatgtc
    15361 cacctggatc ctccaaccta aaggcggcca aagcgtttgc gccgcaggcg actgccgtat
    15421 cgacatcaaa cgccgcagcg acaagctgta cagcgcaagc gtcagcgtgc ctttagccgc
    15481 tatccaagcc ggcgccaaag ccgaaactgc cgtcaacctg tatgccggtc cgcaaaccac
    15541 atccgttatc gcaaacatcg ccgacaacct gcaactggca aaagactacg gtaaagtaca
    15601 ctggttcgca tcgccgctct tctggctcct gaaccaactg cacaacatta tcggcaactg
    15661 gggctgggca atcgtcgttt tgaccatcat cgtcaaagcc gtactgtatc cattgaccaa
    15721 cgcctcctac cgttcgatgg cgaaaatgcg tgccgccgca cccaaactgc agaccatcaa
    15781 agaaaaatac ggcgacgacc gtatggcgca acagcaagcg atgatgcagc tttacaaaga
    15841 cgagaaaatc aacccgctgg gcggctgtct gcctatgctg ttgcaaatcc ccgtcttcat
    15901 cggcttgtac tgggcattgt tcgcctccgt agaattgcgc caggcacctt ggctgggctg
    15961 gattaccgac ctcagccgcg ccgaccccta ctacatcctg cccatcatta tggcggcaac
    16021 gatgttcgcc caaacctatc tgaacccgcc gccgaccgac ccgatgcagg cgaaaatgat
    16081 gaaaatcatg ccgttggttt tctccgtcat gttcttcttc ttccctgccg gtttggttct
    16141 ctactgggtg gtcaacaacc tcctgaccat cgcccagcag tggcacatca accgcagcat
    16201 cgaaaaacaa cgcgcccaag gcgaagtcgt ttcctaaatg ccgcagcatg aaaaatgccg
    16261 tctgaaacct gttcagacgg catttttatt gttcaccccc tatcggggcg gaaatcttca
    16321 acccgcatac atcacaaaaa tcgtcgggcg ttttttcaga ttgggcattt ccttcatcct
    16381 gcgccaatcc gcaaccgttt tactgaccac caactgagtc ggcaacgtca aatccgtagc
    16441 cgtacacaag cgcgtttcag gatgcaggtt ttccaccgca tcggcaagca gcgcatcatt
    16501 gcggtaaggc gtttcaataa aaatctgcgt ctcgccgcac tgccgcgaac gttgctccaa
    16561 agcattcaaa ctctgaatgc gctcattttt ttcagacggc agataaccat taaacgcaaa
    16621 gttctgccca ttcgcacccg aagccatcag cgccagcagc aggctggacg ggccgaccaa
    16681 aggccgtact tcaaaaccgt gtttatgcgc caatgccacc aaattcgcgc ccggatcggc
    16741 aacagccggg caacccgcct cgctgacaat ccccatactg cgcccttctt gcaaaggttt
    16801 cagcaattcc ggcaaagtct tcaaatccgt gtgttcattc aatgtttgca gattcagttc
    16861 gcgtatcggc gtcgttatcc ccaaatgctt caaatgcgca cgcgccgttt tttccgcttc
    16921 cacgacaaaa tccgtcagcc cgacaaccgc ccgttgttca tgctgcaaca ggcacggcgt
    16981 gtcaggcgta cccaaaggcg taggaatcaa atacaaaaca ggagacatca ttccctcact
    17041 catcggttaa aaatgccgtc cgaacccttc agacggcata aacgggcagt tacaaaacat
    17101 ccacgccctc gtttttcaaa aaatcgacca gacggaaaac cggcagacca atcaaggcat
    17161 tcggatcggt actttcaatc cgttcgatca acagcgcgcc caagccctcg cttttcgccg
    17221 cacacgaaca ataaaccgca tccggctcgc gctccaaata gcggaggatg tgcagctcgt
    17281 ccaactgcct catcacgacc accgtcttat cgatatgccg atgcatcctg cccgtaaccg
    17341 tatttagcag aacgaccgca ctgtaaaact caatctccct gccgctcaaa tgcatcaaca
    17401 tcttttgcgc gttggcaagg ttcatcggct tgccccactg cctgccgtcg caccacgcca
    17461 cctggtccgc accgacaatc aacgccccgg ggaaacgccc ggtcaacgac cgcgccttac
    17521 cctcggcaag gcgcaatgcc gtctgagggg cggattcccc caacatcggc gtttcgtcaa
    17581 aatcggggga tgccgcctga aaggcaatgc cgagcctttc catctgttcg cggcggaaaa
    17641 ccgaactcgt acccaaaacc aaaggcagtt ccaaacccat cccatcctcc ttaccgttga
    17701 aaacacgccc gaaggggcgg taaaatccag ccatgcgccg aaacacggac gcccgccttc
    17761 ggcgtaccgc aacatttttc ttaaaaatat tgacgttaga acatctaaat tatatcatac
    17821 cccgtttatg tcagacccta atttgattga cccggaaatt tttgccgccg aaaggcagaa
    17881 cctgcaaggc agttttctgc tggaagaatt ggacgagcga gtcagtttgc acgattatcc
    17941 cgccgacagg cggaccaaaa tatcgtttac actgaccggc ggtcgcgacc ggctgcaacg
    18001 cctgttcctc gacctgaacg tcaaagccga tatgcccctg atttgccaga gatgtatcaa
    18061 acccatgccg ttcatgctcg atgaaagcag ccgtatcatc ctgttttccg acgaagagtc
    18121 cttggacgaa tccatgcttg ccgacgaaga actcgaaggc atactgattg aaaaagaact
    18181 cgacgtgcgc gcattggtag aagaccaaat cctgatgtcc ctgccctttt cgccgcgaca
    18241 cggacactgc ggcaataccc ttccggaatc cgccaaccaa gacaaaccca acccctttgc
    18301 tgttttggcg ggtttgaaaa gcagttaatt aggacacagt ttatttatct aggagcttga
    18361 aatggccgtt caacaaaaca aaaaatcccc ttccaaacgc ggtatgcacc gttcgcacga
    18421 cgcactgacc gcgcccgcac tgtttgtcga cagcacaacc ggcgaagtac accgcccgca
    18481 ccacatctcc cccaacggta tgtaccgcgg ccgcaaagtg gtcaaagcca aaggcgaata
    18541 atccctattc gactgactga aaaagccaga acattgccat gcaattactg gctttttttg
    18601 cattggacgc accatccgtc caaactttcg ccatacgtca acacacaggg gcaaagcgtt
    18661 ccgtataata ccccgtgaaa atattccaaa agccccaacc accaaggaaa ttccgatgaa
    18721 acagaaaatc tggtacacct acgatgacat ccaccgcgtc atcaaagcat tggcagaaaa
    18781 aatccggaac gccggcgtca aatacgatgc catgatcgct atcggcggcg gcggctttat
    18841 tccggcacgc atgctgcgct gttttctgga aattccgatt tatgccgtaa ccaccgccta
    18901 ttacgacagc gacagcgaag gacaagttac cgaagaagtc aaaaaagtcc aatggctaga
    18961 ccccgtcccc gaagtcctgc ggggcaaaaa cgtgctcgtc gtcgatgaag tggacgacag
    19021 ccgcgtaacc atggagttct gcctgaaaga actgctcaag gaagacttcg acactgtcgg
    19081 cgtcgccgtc ctgcacgaaa aaatcaaagc caaagcaggc aaaatccccg aaggcattcc
    19141 ctatttcagc ggcattaccg tagaagactg gtggatcaac tatccgtggg acgcactcga
    19201 catcgacgaa cacaaccgcc ttgccgaagc cggccgaggc taaccctttc agacggcata
    19261 ttttccgaac cgatgccgtc tgaagcccgc acgacccctg ccgcagaccg aaaatctacc
    19321 ggagaaaccc ctatgattac attggccgta gatgccatgg gcggcgacca aggacttgcc
    19381 gttaccgtac ccggcgcaac cgcattcctc caagcacacc ccgatgtccg cctgattatg
    19441 accggcgacg aaacgcagct ccgccaagcc ctgaacgcgg caggcgcgcc gatggaacgc
    19501 atcgacatct gccataccac ccaagttgtc ggcatggatg aatcccctca gtccgcccta
    19561 aagaacaaaa aatactcctc catgcgcgtt gccatcaacc aagttaaaga aggcaaagcc
    19621 caagccgccg tatccgcagg caacacgggc gcgctcatgg caaccgcacg tttcgtcctc
    19681 aagaccatac ccggcatcga acgccccgcc atcgccaaat tccttccttc cgacaccgac
    19741 cacgttaccc tcgccctcga cctcggcgca aacgtcgact gcacacccga acagcttgcc
    19801 caatttgccg tcatcggcag cgaactcgtc cacgcactcc atcctcaaaa aggacagccg
    19861 cgcgtcgggc tggtcaacgt cggcacagaa gacatcaaag gtacggatac cgtcaaacaa
    19921 acctacaaac tgctgcaaaa cagcaaactc aactttatcg gcaacatcga aagcaacggc
    19981 atcctgtacg gcgaagcaga tgtcgtcgtc gccgacggct ttgtcggcaa cgtcatgctc
    20041 aaaaccatcg aaggcgcagt caaattcatg agcggagcca tccgccgcga attccagagc
    20101 aacctgttca acaaacttgc cgccgttgcc gccctacccg ccctcaaagg cttgaaaaac
    20161 aaactcgatc cgcgcaaatt caacggggcc atcctgctcg ggctgcgcgg catcgtcatc
    20221 aaaagccacg gcggcacaga caaaaccggt ttccgctacg cactcgaaga agcctaccac
    20281 gaagccaagt ccgccggcct ttccaaaatc gaacaaggcg ttgccgaaca actcgccgca
    20341 ctggaagccg cccaaaacga aaccgccgcc agtctgtaac acgcacgatg ccgtctgaac
    20401 gcccccgccc ctttcagacg gcatccggca cccgtccaaa cctgcgggcg cggacggcga
    20461 tgcgcctgtc cggcacttcc caaatacttc cctgtaaaat aaggagtatt tgaaaaatga
    20521 agacattaga aaaacggatg aaagctctag acaaacggat gatgaagttc ggaaaatccc
    20581 ttgaaggcag gcttgatgcc cgtctgattg aatccgcatt ggattatatt cattattcgg
    20641 aacgtttttt ggcttttgac atcctgtgta cttatatcga agatttcgat atccggctga
    20701 cggaacaaga atcccgggaa atttctttta tcaacaagga atttgagata gaaagcacgt
    20761 ccgattaacc aataaagcca atgatttgat aagaatgaaa acatcgacgg tcgtttttgg
    20821 cggatttttt atggcagaca acggggagcg aatccaaatc cccgttttgg aaaatcctga
    20881 cattagggaa atcaatcgct ttttttccgt atcaaatttt gagaaaaaag ccggcgtcct
    20941 tgttttcaga atcatccccg agccggaatt tggcaatacc gaattaactg tctattttga
    21001 aaaaggatat tatagtggat taacaaaaac cggtacggcg ttgccccgcc ttggctcaaa
    21061 gagaacgatt ccctaaggtg cccaagcacc aagcgaaccg gttccgtact atccgtactg
    21121 tccgcggctt cgccgccttg tcctgatttt tgttaatcca ctatatttgc caataattca
    21181 aacaatttta gaaggcgggg atattaaagt aaaaaacctt aaaacagaaa attacagcgg
    21241 gaaggcaagg gaaatcttgg gagattccta ccctattgaa catatctcta aaaatatttc
    21301 tacaattcaa aatatcattt ccgaatttat cagacaaaaa caaacaccgg caccaaataa
    21361 tttgatggaa acccaacaat caagatgccg tctgaagcag cttgggcttt cagacggcat
    21421 ttccttcact taaaacagcg tatcggcaac cccgccccgc ccgtccacgg caatccgcat
    21481 ctgaaaacca tctgtatgcc aaaccacacc cccatccctg tttccgtcat ttgcaccctg
    21541 cccgtattgg gcaatcatcg gtttttcgct tacaatagcc gaatctgaac caactctcta
    21601 aaaaggccgt tcccatgcag tatgcaaaaa tttccggcac gggcagctat cttcctgcca
    21661 accgcgtcag caatgacgac cttgcccaaa aggtagatac ctctgacgag tggattaccg
    21721 cgcgtacggg catcaaattc cgccatattg cagccgaaaa cgaaaaaacc agcgatcttg
    21781 cagccgaagc ggcgcggcgc gctttggcag atgccaaatt aaatataaac gacattgatt
    21841 tgattatcgt ggcgacggca acgccggata tgcagtttcc ttctaccgcc accatcgtgc
    21901 agcaaaaatt gggcatcacc aacggctgcc ccgcgtttga cgtgcaggcg gtgtgcgcag
    21961 gctttatgta cgcgctgact acggcaaatg cctacattaa aagcggtatg gcgaaaaacg
    22021 cgctggtcat cggcgcggaa accttcagcc gcatcgtcga ttggaacgac cgcacaacct
    22081 gcgtgctgtt cggcgacggc gcgggcgcgg tggttttaag cgcgtccgac aaaccgggca
    22141 tcatccacag caaactcaaa gccgacggca attatctgaa actcttaaac gtccccgggc
    22201 aaatcgcctg cggcaaagtt tccggttcgc cgtacatttc gatggacggt cccggcgtgt
    22261 tcaagtttgc cgtcaaaatg ctgtccaaaa tcgccgatga cgttatcgaa gaagcaggtt
    22321 acaccgccga acaaatcgac tggattgttc cccatcaggc aaaccgccgc attatcgaat
    22381 cgaccgcgaa acatttgggt ttgagtatgg acaaagtcgt cctgaccgtc caagaccacg
    22441 gcaacacgtc cgccgcatcc attccgctgg ctttggacgc gggcatccgc agcggacaaa
    22501 tcaaacgcgg tcaaaacctg ctgctcgaag gcataggcgg cggtttcgcg tggggcgcgg
    22561 tgctgttgca atattgaaac cggtgccgtc tgaaacaggc tttcagacgg catttcccat
    22621 atcatgaagc ggcaggcttt cttcaaaccg atggcgtgtg cggcatttct gtccgccgtt
    22681 tcgctgcgcc tccccgtatt gggcgcgtgt tacgcaatat tgtccctcta tgcgtttgca
    22741 ctttacggca tcgacaaacg gcgtgccgtg cggggaaaac gccgcattcc cgaacaccgc
    22801 ctgctcctgc ctgccttgtt cggcggttgg acgggcgcat acttgggtag taggatgttc
    22861 aggcataaaa cggcgaaaaa gcgttttgtt gtgctgttcc gtctgactgt ttcgggcaat
    22921 gtcctggcga cctgcatcct gattgattat ttcgttccgc ccgaactttt tgtaaaactc
    22981 gggcaacatc tctgacaatc caacccaaca aaggaatact atgtctttcg cattcttctt
    23041 ccccggacaa ggttcccaaa gcctcggcat gatgaacggc tttgccgaac acgccatcgt
    23101 caaaaacaca tttgacgaag cctccgccat attggggcag gacttgtggg cgatgattaa
    23161 cggcagcgat gccgaaatca tcggtcaaac agtcaacacc cagcccatta tgctcgccgc
    23221 cggcgttgcc gtttaccgcg cctatttgga agtgggcggc aaaacgcctg ccgccgttgc
    23281 cggacacagc ctcggcgaat acaccgcgct tgtcgccgcc gaagcattgg attttgccga
    23341 tgccgtcaaa ctcgtccgcc tgcgcgccga actgatgcag tccgccgtac cgcaaggcgt
    23401 aggcgcaatg gcggccattt tgggtttgga agatgaacag gtcaggcaga tttgtgccga
    23461 atccgcacag ggcgaagtgg tcgaagccgt caacttcaac tcgcccggac aagtcgtgat
    23521 tgcaggcaac gccgccgccg tcgggcgcgc catggctgcc gccaaagaag ccggagccaa
    23581 acgcgccctg ccgctgccgg tatccgttcc ttcccattgc agcctgatga aacccgccgc
    23641 cgacaaactt gccgaagcac tcaaaaccgt tgaaatcaaa cagccgcaaa tccgcgtcat
    23701 ccacaacgcc gacgttgccg cctacgatga tgccggcaaa atcaaagacg cgctcgtccg
    23761 ccagctttac agccccgtgc gctggacgga aaccgtcaac gccctcgttt cagacggcat
    23821 tgccgaatcc gccgaatgcg gcccgggcaa agtgttggcg ggcttggcaa aacgcatcaa
    23881 caaggccgcc gcgtgcagcg cactgaccga tgccggacag attaccgcct ttatcgaagc
    23941 gcactgactt cgttccgcaa aaagcagcct gccctcttca ggctgctttt cacgtccgaa
    24001 cggcaaacag ccgcatattt gcgctataat ccatccctac caaaccaccg acagcggctg
    24061 ccgttgcagt tcccgcccta ccgatatgat agaaaaactg actttcggac tgtttaaaaa
    24121 agaagacgcg cgcagcttta tgcgcctgat ggcgtacgtc cgcccctaca aaatccgcat
    24181 cgttgccgcc ctgattgcca ttttcggcgt tgccgccacc gaaagctatc ttgccgcctt
    24241 catcgccccc ctgatcaacc acggcttttc cgcgcccgcc gcgccgcccg atctgtccgc
    24301 cgccgccggc atcctttcca ccctgcaaaa ctggcgcgaa cagtttacct atatggtttg
    24361 ggggacggaa aacaaaatct ggaccgtccc gctcttcctc atcatcctcg tcgtcatccg
    24421 tggcatctgc cgctttacca gcacctatct gatgacttgg gtctccgtga tgaccatcag
    24481 caaaatccgc aaagatatgt ttgccaaaat gctgaccctt tcctcccgct accatcagga
    24541 aacgccgtcc ggcaccgtac tgatgaatat gctcaacctg accgaacagt cggtcagcaa
    24601 cgccagcgac atcttcaccg tcctcacgcg cgacacgatg atcgttaccg gcctgaccat
    24661 cgtcctgctt tacctcaact ggcagctcag cctcatcgtc gtcctgatgt tccccctgct
    24721 ctccctgctc tcgcgctact accgcgaccg tctgaaacac gtcatttccg actcgcaaaa
    24781 aagcataggc acgatgaaca acgtgattgc cgaaacccat cagggacacc gcgtcgtcaa
    24841 gctgttcaac gggcaggcgc aggcggcaaa ccggttcgac gcggtcaacc gcaccatcgt
    24901 ccgcctcagc aaaaaaatca cgcaggcaac ggcggcacat tccccgttca gcgaactgat
    24961 tgcctcgatc gccctcgccg tcgtcatctt catcgccctg tggcaaagcc aaaacggcta
    25021 caccaccatc ggcgaattta tggcattcat cgtcgcgatg ctgcaaatgt acgcccccat
    25081 caaaagcctt gccaacatca gcatccctat gcagacgatg ttcctcgccg ccgacggcgt
    25141 atgtgcattt ctcgacaccc cgcccgaaca ggacaaaggc acgctcgcgc cgcagcgtgt
    25201 cgaagggcgc atcagcttcc gcaacgtcga tgtcgaatac cgttcagacg gcatcaaagc
    25261 cctcgacaac ttcaacctcg acatcagaca aggcgaacgc gtcgccctgg tcggacgttc
    25321 cggcagcggc aaatccaccg tcgtcaacct tctgccccgc tttgtcgaac cgtctgccgg
    25381 caacatctgc atagacggta tcgacatcgc cgacatcaaa ctcgactgcc tgcgcgccca
    25441 attcgccctc gtctcccaag acgtattcct gtttgacgac accctgtttg aaaacgtccg
    25501 atacagccgt cccgacgcgg gcgaagccga agtcctgtcc gccctccaag ccgccaacct
    25561 gcaaagcctg atcgacgcat ccccgctcgg actgcaccag cccatcggat cgaacggcag
    25621 caacttgtcc ggcggacagc ggcaacgcgt cgccatcgcc cgcgccattt tgaaagacgc
    25681 gccgatatta ttattggacg aagccaccag cgcattggac aacgaatccg aacgcctcgt
    25741 ccaacaagcg ctcgaacgcc tgatggaaaa ccgcaccggc atcatcgtcg cccaccgcct
    25801 gaccaccgtc gaatccgccg accgcatcat cgtcatggat ggcggcaaaa tcatcgaaca
    25861 aggcacacac gatcaactca tgttccaaaa cggctactac accatgctga ggaacatctc
    25921 cggaaaagat accgccgccg ttcagacggc ataaacaaaa tgccgtctga aatggtacaa
    25981 tcatccaaat cccttcagac ggcactatat ccgccgaccc atccgattat cttcaaccac
    26041 cgtaaaaccc attatgaccc aagacaaaat cctcatcctc gacttcggtt cgcaagttac
    26101 ccggctgatc gcccgccgcg tgcgcgaagc acacgtttac tgcgagctgc attccttcga
    26161 tatgcctttg gacgaaatca aagccttcaa ccccaaaggc atcatccttt ccggcggccc
    26221 taattctgtt tacgaatccg actatcaagc cgataccggt atttttgatt tgggcattcc
    26281 ggttttgggc atctgctacg gcatgcagtt tatggcgcac cacttgggtg gcgaagtgca
    26341 gcccggcaac cagcgcgaat tcggttacgc gcaagtcaaa accatcgaca gcggactgac
    26401 acgcggcatt caagacgacg cgcccaacac actcgacgta tggatgagcc acggcgacaa
    26461 agtgtccaaa ctgcccgacg gtttcgccgt catcggcgac actccgtcct gcccgattgc
    26521 catgatggaa aacaccgaaa aacaattcta tggcatccag ttccaccccg aagttaccca
    26581 caccaaacaa ggccgcgccc tgttgaaccg ctttgtcttg gatatttgcg gcgcgcaacc
    26641 gggctggacg atgcccaact acatcgaaga agccgttgcc aaaatccgcg aacaagtcgg
    26701 cagcgacgaa gtgattttag gtctgtccgg cggcgtggac tcttccgtag ccgccgcgct
    26761 gattcaccgc gccatcggcg accaactgac ctgcgtgttc gtcgatcacg gtttgttgcg
    26821 cctgaacgaa ggcaaaatgg tgatggatat gttcgcccgc aacttgggtg tgaaagtgat
    26881 acacgtcgat gccgaagggc agtttatggc gaaactcgcc ggcgtgaccg accctgagaa
    26941 aaaacgcaaa atcatcggcg cggaatttat cgaagtattt gatgccgaag agaaaaaact
    27001 caccaacgcc aaatggctgg cgcaaggcac gatttacccc gacgtaatcg aatccgccgg
    27061 tgcgaaaacc aaaaaagccc acgccatcaa atcccaccac aacgtcggcg gcctgcctga
    27121 aaatatgaag ctcaaactgc ttgagccctt gcgcgacttg ttcaaagacg aagtgcgcga
    27181 gttgggcgtg gctttgggcc tgccgcgcga aatggtgtac cgccacccct tcccgggccc
    27241 cggtttgggt gtgcgcatct tgggcgaagt gaaaaaagaa tacgccgact tgctgcgtca
    27301 ggcggacgat attttcatcc aagaattacg caatactacc gacgaaaacg gcacgtcttg
    27361 gtatgacctg accagccagg catttgccgt gttcctgccc gtcaaatccg tcggcgtgat
    27421 gggcgacggc cgcacttact actacgtcgt cgcactgcgc gcagtcatca ccagcgactt
    27481 tatgactgca cactgggcag agctgccata ctcactgctc ggccgcgtgt ccaaccgcat
    27541 catcaacgaa gtcaaaggca tcaaccgcgt ggtgtacgat gtcagcggca aaccgcccgc
    27601 caccatcgag tgggaataaa cagcaaacag ggctgcaccg tccggcgcag tccttcgatt
    27661 atcggaaaaa aggaaaaaat atgagcacac aagatttaag cggcaaaatc gctttggtaa
    27721 ccggcgcatc gcgcggcatc ggcgcggcaa ttgccgacac actggcggca gccggtgcca
    27781 aaatcatcgg tacggcaacc ggcgagagcg gtgcggcggc gattagcaaa cgtctggcac
    27841 aatggggcgg cgaaggccgc gtattaaatt ccgccgaacc cgaaaccgtc gaaaacctga
    27901 ttgccgacat cgaaaaaacg ttcggcaaac tcgacattct ggtcaacaac gccggcatca
    27961 cccgcgacaa cctgttgatg cgtatgaaag aagaagagtg ggacgacatc atgcaggtca
    28021 acctcaaatc cgtgttccgc gcttctaaag ccgtcttgcg cggcatgatg aagcagcgcg
    28081 ccggccgcat catcaacatc acctccgtcg tcggcgtgat gggcaatgcc gggcaaacca
    28141 attatgccgc cgcaaaagcg ggcttgatcg gttttgccaa atctatggcg cgcgaagtcg
    28201 gcagccgggg cattaccgtc aactgcgtcg ccccgggctt tatcgatacc gatatgaccc
    28261 gcgccctgcc cgaagaaacc cgccaaacat ttaccgccca aaccgccttg ggcagattcg
    28321 gcgacgcgca agacatcgcc gatgcggttc tgttcctcgc ttccgatcag gcgaaataca
    28381 tcaccggtca aacgctgcac gtcaacggcg gtatgctgat gccctaacgg acaggctttt
    28441 caaccatgcc gtctgaagcc ctttcagacg gcatttgcat tcccaaacaa aatgaacact
    28501 caccaccacc tgccctgccc ctgcggttca ggcacaagct atgccggctg ctgccgaccg
    28561 cttcatctgc gccaaatcct gccgcccacc gccgaagccc tcatgcgttc gcggtacggc
    28621 gcgtatgtgc tgcaccttat cgactacatc atcgctacta ccgttccggc gcaacaaaca
    28681 ttcctcgatg ccgccgaact gatgcagtgg agcagagaag ccgaatggct gggcttgaat
    28741 gtcatcgcac accgcaacct cggcaaacag cacgcccaag tcgaatttga agcctacttc
    28801 caagacggcg gaaaccgaac agtgcatcac gagctgtcgg cattcgtgaa aatagcggag
    28861 caatggtatt tcatcgatcc gactgttccg ctgcctacca tgaaacagcc ctgcatttgc
    28921 ggatcgagca aaaaattcaa agcctgctgc ggcaaatatc tgcaacctgt cgcataaaaa
    28981 tgccgtctga acgttcagac ggca
//
LOCUS       DAXFZR010000015        28193 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000015 DAXFZR010000000
VERSION     DAXFZR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28193
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.015"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(115..294)
                     /locus_tag="ACFJAS_000576"
     CDS             complement(115..294)
                     /locus_tag="ACFJAS_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC3981897.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            complement(300..887)
                     /locus_tag="ACFJAS_000577"
     CDS             complement(300..887)
                     /locus_tag="ACFJAS_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC3981898.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(909..1655)
                     /locus_tag="ACFJAS_000578"
     CDS             complement(909..1655)
                     /locus_tag="ACFJAS_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC3981899.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSPPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            complement(1645..2487)
                     /locus_tag="ACFJAS_000579"
     CDS             complement(1645..2487)
                     /locus_tag="ACFJAS_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC3981900.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELAYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            complement(2606..2914)
                     /locus_tag="ACFJAS_000580"
     CDS             complement(2606..2914)
                     /locus_tag="ACFJAS_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981901.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEDKCGEGKCGSK
                     "
     gene            complement(2988..3437)
                     /locus_tag="ACFJAS_000581"
     CDS             complement(2988..3437)
                     /locus_tag="ACFJAS_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC3981902.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKLFCPKCRLKQD"
     gene            3779..4672
                     /locus_tag="ACFJAS_000582"
     CDS             3779..4672
                     /locus_tag="ACFJAS_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC3981903.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYFDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            4775..5878
                     /gene="prfB"
                     /locus_tag="ACFJAS_000583"
     CDS             4775..5878
                     /gene="prfB"
                     /locus_tag="ACFJAS_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC3981904.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            5957..6316
                     /gene="rnpB"
                     /locus_tag="ACFJAS_000584"
     ncRNA           5957..6316
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFJAS_000584"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6646..8103)
                     /locus_tag="ACFJAS_000585"
     CDS             complement(6646..8103)
                     /locus_tag="ACFJAS_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3981905.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(8884..13053)
                     /locus_tag="ACFJAS_000586"
     CDS             complement(8884..13053)
                     /locus_tag="ACFJAS_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC3981906.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     AVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPGTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AEHVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEAFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(13108..14955)
                     /locus_tag="ACFJAS_000587"
     CDS             complement(13108..14955)
                     /locus_tag="ACFJAS_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC3981907.1"
                     /translation="MHDTRTMMIKPTSLLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRTGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            15326..16555
                     /gene="sstT"
                     /locus_tag="ACFJAS_000588"
     CDS             15326..16555
                     /gene="sstT"
                     /locus_tag="ACFJAS_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC3981908.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILAAATIAQHQKGNKVHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            16706..17986
                     /locus_tag="ACFJAS_000589"
     CDS             16706..17986
                     /locus_tag="ACFJAS_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC3981909.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHMENGRPYP
                     SGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKDRD"
     gene            17986..18189
                     /locus_tag="ACFJAS_000590"
     CDS             17986..18189
                     /locus_tag="ACFJAS_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC3981910.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            18249..18497
                     /locus_tag="ACFJAS_000591"
     CDS             18249..18497
                     /locus_tag="ACFJAS_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981911.1"
                     /translation="MFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKGIAFPNRGGAT
                     GCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(18490..18705)
                     /locus_tag="ACFJAS_000592"
     CDS             complement(18490..18705)
                     /locus_tag="ACFJAS_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981912.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(18698..18913)
                     /locus_tag="ACFJAS_000593"
     CDS             complement(18698..18913)
                     /locus_tag="ACFJAS_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC3981913.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(19116..20459)
                     /gene="argG"
                     /locus_tag="ACFJAS_000594"
     CDS             complement(19116..20459)
                     /gene="argG"
                     /locus_tag="ACFJAS_000594"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC3981914.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            20584..21384
                     /locus_tag="ACFJAS_000595"
     CDS             20584..21384
                     /locus_tag="ACFJAS_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC3981915.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAKLKNRVN"
     gene            21424..22410
                     /locus_tag="ACFJAS_000596"
     CDS             21424..22410
                     /locus_tag="ACFJAS_000596"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC3981916.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            23071..23322
                     /locus_tag="ACFJAS_000597"
                     /pseudo
     CDS             23071..23322
                     /locus_tag="ACFJAS_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(23317..23513)
                     /locus_tag="ACFJAS_000598"
                     /pseudo
     CDS             complement(23317..23513)
                     /locus_tag="ACFJAS_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            23615..23998
                     /locus_tag="ACFJAS_000599"
                     /pseudo
     CDS             23615..23998
                     /locus_tag="ACFJAS_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(24036..24410)
                     /locus_tag="ACFJAS_000600"
     CDS             complement(24036..24410)
                     /locus_tag="ACFJAS_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC3981917.1"
                     /translation="MIIQNEFNLYPSNMLPEGFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(24584..24940)
                     /locus_tag="ACFJAS_000601"
     CDS             complement(24584..24940)
                     /locus_tag="ACFJAS_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC3981918.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(24937..25785)
                     /gene="mafB"
                     /locus_tag="ACFJAS_000602"
                     /pseudo
     CDS             complement(24937..25785)
                     /gene="mafB"
                     /locus_tag="ACFJAS_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(25890..26105)
                     /locus_tag="ACFJAS_000603"
     CDS             complement(25890..26105)
                     /locus_tag="ACFJAS_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981919.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQLVQELEQHLIEAYNLMDTSN"
     gene            complement(26229..26588)
                     /locus_tag="ACFJAS_000604"
     CDS             complement(26229..26588)
                     /locus_tag="ACFJAS_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC3981920.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            complement(26585..27337)
                     /locus_tag="ACFJAS_000605"
                     /pseudo
     CDS             complement(26585..27337)
                     /locus_tag="ACFJAS_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(27577..27783)
                     /locus_tag="ACFJAS_000606"
     CDS             complement(27577..27783)
                     /locus_tag="ACFJAS_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981921.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(27793..>28193)
                     /gene="mafB"
                     /locus_tag="ACFJAS_000607"
     CDS             complement(27793..>28193)
                     /gene="mafB"
                     /locus_tag="ACFJAS_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3981922.1"
                     /translation="TPAVRTMHTLDGEMAGGNRPPKSITSNSKADASTQPSLQAQLIG
                     EQISSGHAYNKHVIRQQEFMDLNINSPADFARHIENIVSHPTNMKELPRGRTAYWDDK
                     TGTIVIRDKNSDDGGTAFRPTSGKKYYDDL"
BASE COUNT         6570 a         6792 c         7672 g         7159 t
ORIGIN      
        1 atggaggaat atgctgcttt tgaagagtgt attatggatg aactgccgat aacagtaaat
       61 cggtttgatt tgtttccgtc agcttatgcc gtctgaagcc ctttcagacg gcatttattt
      121 tgaagttctg gttgtttttg ccagtgatat tttgatggtt tgaagctgtc ttttatattc
      181 ccggcaatgc gggcagaaca gcaggtgcat atatatggaa atcttctcgc ccggggtggt
      241 ttcccggtcc tgatgtttgg aaagaagcag ggcaatatcg cggcattttt tcataacgtt
      301 tacttcgggt tttcttggtt gaaccatttg atttgcaggc attggcgcaa tgattctcgg
      361 gcgcggtgca taatggtgtg gtagttggac gtgctgatac cgcacatttg ttgtatttcg
      421 ttggatgaaa aaccgagtat ttccttcagg gtgaataccc gtgcggtgtt ttcaggcagg
      481 ttgtataggc agctttgcag aattttttgg aattcgttgt tatttaatga tttttccgga
      541 gtgttccaat gttgcggctg cccttccggc gtccaatgcc cgttttggga aaaatggctt
      601 tcaaatgctt catccagcag ctcgtcatcc agtgcggtaa agactttcct ctgcctcccg
      661 atttgacgta atgcgtcaat aattttgttt ttcaatatgg caaaaagcca gctgttgaca
      721 agtgccctgc cttgaaaact gtcgcctgcg ctgtatgcgg acagcaatgt ttcctgcact
      781 aaatcttcag ccaaatcagg gtggtcgggc aactggagcc gcgcaaaatg cagaagcagt
      841 ttgcgcgact ccattaattc ggcatcggtc aggtcgggta gcggcatagt atcgggaaca
      901 aggtaatttt atgcggatag atggttttgg ctcggggaat tgccttccat attttcggat
      961 atggcggaca aggaggggat gataatcctt tgttcaatcc agcctgacca tttcccaagc
     1021 aaaatatttt tccaattatc ggctttaggc ataaattcga caagggtttg ggcgagggtg
     1081 tcaaacgaaa gcgcggagga acccattatc tccagcagca tcatatcgaa gccgtccaat
     1141 gtttggtaca tcacatcatc ttcggcgttt cgccatatta acaaggctgt ttccgcttcc
     1201 tgcaaatcat gggtaacatc gtatcgatat tgccggataa aggccgcagg ggagggcgtg
     1261 tatttgctgt catttgaata atgaatgtcg ggaatatccg gaatttgtgc aacttctgcc
     1321 agcaattggg tatattcaaa atccatcagc gccaaaatgc cgtctgaaag cggcgggctt
     1381 tggcaatatt gtaggaactc gccggggatt tcttgaaaat agggcgtttg ggcacgcgcg
     1441 tcgcggacga aaccttcttt cagacggctc cattctttgc tgtcaaagta ttgccgcgtt
     1501 tcggtatagc agcggtcgat aaagctgtgg atattgttgc gtatcaggcg gatatagacg
     1561 ttcaatcggt cttgcggcag accgtctgcg gcttcgcccc cgcgtatggc ttgggagaaa
     1621 cggtgctggt attgggcgga ggtttcaggc tgcacggcgg tattcctttc cggcacgcgt
     1681 ttgataatcg gcaattttgg caacttcggc ttcgagttcg gcaaaaggtg ggaaattgaa
     1741 atcgcgttcc aacagggtgg gagggatggt gggaagcttg gtgtaggcga gttcgagcaa
     1801 gtcccaaacg gtcggtaaaa ccgccgcgcc gtgggtatca atcaaaagct cgggggtttc
     1861 ggcatcgtgt ccggcgatgt ggatgtagca cacgcgccct gcgtccacat tctctaaaaa
     1921 ggcctcaggc gacagcaggc cgtgattgac ggcgttgacg taaatattgt tcacatccaa
     1981 atgaataccg cagtcggctt cacgggcaac cgcgttgagg aactcgacct cgttcatctc
     2041 ggcaagcgga gaatgcaggt agtaggacgt gttttccacg gcgatgcggc agcccaaacg
     2101 gtcttgcact tcgcggatac gccgcgccgt atgatgcacc atttcttcag tgaaaggcag
     2161 cggcaacaaa tcgtaaagat gaccgccgtc gtggcagtag ctcaaatgat cggagaaaaa
     2221 cgtgcagtca taacggcaca tcatttcttt gatgccgtct atcaaatcgg tatccagcgg
     2281 ggcttgtccg cccagcgaca tagataatcc gtgcaacgcc agcggcagcc gttccgccac
     2341 acggtcaaac tgtttgcgcg ccctgccgcc cattttcagc cagttttccg gtgcggcttc
     2401 gataaagcat atcgggctgt tttcggaaag cgagagaaag tcttccgcca aatcgcggcg
     2461 gtagcccaag cctgcgtgtt gaatcatatt tttacccggt tcggatggca caaaagaatg
     2521 atgtgcttgt taaaaaaaaa tctttgtttg ccgttaaaaa agccgcattt tactgaaaaa
     2581 cgcggcttgg tttgaagggg cgggattatt tagaaccgca tttgccttcg ccgcatttgt
     2641 cttcggcaga tttggctttg gctttagatg ctttggtgtg tttgtgggct ttttttacag
     2701 ttgcaccgca tttgccctcg ccgcatttgc cttcgcctgc tttagaagca gccgcgccgc
     2761 acgaaccttc ggcagatttg gatgcgccgc aagagccttg ggcggatttt tgaacgcctg
     2821 ttgcgttgct tgccggtttg tgggcggcaa cggcgccggc ggccagagac agggataaag
     2881 caccggcgag tgcggcagca atatttttgt tcatttctat ttccttttca gaatatcaag
     2941 ttaacgagat aaggttggaa tgtttaaagt cggattccac gactgaatca atcttgtttc
     3001 agacggcatt tggggcaaaa cagttttttc agcagcgtat ccagcgacca tccgcccgca
     3061 ccctggaaaa gcagcgggat taataccacg atataaatta aagccatttt ataaccgttg
     3121 tcgcagacat tgtaacccga accggcgtga accgcagccc aagcgacggc ggtaacgacc
     3181 atcagcccta atgccgacag acgcgttgcc aaacccaaaa gcaacagtac gggcagtaaa
     3241 agctccgcat acatagcgag attccagttt aacgcgtccg gcagcaagtt gaacgggaat
     3301 gggaattgat cgttgatttc agaaaaccaa ttctccccgt tccatttttg caaacccgat
     3361 tccaaaaatt catatgcggc aaacagacgc aaaaccagca tagcggatgc cgtctgccaa
     3421 gttgttccga ttttcataag tgttcctgtt ttggttctgt tgatacccca ttagacggat
     3481 tttgtcggaa aaactgacac ccggcagcca atgatttaac aagttaatga atataaaaag
     3541 aaagtatttt gatggaaaga aagggaaaaa ggcgggatat ttggataacg gcggtgttgt
     3601 gagataattc ccgccttgga tagtatgcct tcaaatataa tttccgtaaa ggaaaaaaag
     3661 aaatattaat tttgatgatg acttaacaaa gcccccattt gcttggctgt caaagggggt
     3721 tgttaagaaa agcaatccct ctttttgacg gggtgcgata tataaaggaa tggaaaatat
     3781 ggtaacgttt tcaaaaatca gaccgctttt ggcaatcgcc gccgccgcgt tgcttgccgc
     3841 ctgcggcacg gcgggaaata atgccgcccg caagccggtg caaaccgcca aacccgccgc
     3901 agtggtcggt ttggcactcg gtggcggcgc atctaaagga tttgcccatg taggtattat
     3961 taaggttttg aaagaaaacg gtattcctgt gaaggtggtt accggcacat cggcaggttc
     4021 gattgtcggc agcctttttg catcgggtat gtcgcccgac cgcctcgaat tggaagccga
     4081 aattttaggc aaaaccgatt tagtcgattt aaccttgtcc accagtggtt ttatcaaagg
     4141 cgaaaagctg caaaattaca tcaaccgaaa agtcggcggc aggcggattc agcagtttcc
     4201 catcaaattt gccgctgttg ctactgattt tgaaaccggc aaggccgtcg ctttcaatca
     4261 ggggaatgcc gggcaggctg tgcgcgcttc cgccgccatt cccaatgtgt tccaacccgt
     4321 tatcatcggc aggcatacat atgttgacgg tggtctgtcg cagcccgtgc ccgtcagtgc
     4381 cgcccggcgg cagggggcga atttcgtgat tgccgtcgat atttccgccc gtccgggcaa
     4441 aaacatcagc caaggtttct tctcttattt cgatcagacg ctgaacgtaa tgagcgtttc
     4501 cgcgttgcaa aatgagttgg ggcaggcgga tgtagttatc aaaccgcagg ttttggattt
     4561 gggtgcagtc ggcggattcg atcagaaaaa acgcgccatc cggttgggtg aggaggcagc
     4621 acgtgccgca ttgcctgaaa tcaaacgcaa actggcggca taccgttatt gacgctatgc
     4681 cgtccgaacg tgtttccaga aggctttatc catagaaaaa aatacccgaa tctcgtacaa
     4741 tacgccccta aaattttgaa agaacaaaag aatcatggaa gccgaagtaa tcaaccagct
     4801 caacaatacc ctgaatgatt tggaaaagcg cagcgaagac atccgcgttt atatggatta
     4861 tcagggcaag aaagaccgat tggaagaagt catcgggctt tccgaagacc ccgagctttg
     4921 gaacgacccg aaacgtgccc aagaaatcgg caaagagagc aaaatccttg aaggcatcgt
     4981 gctgacgctc gacaacatcg cttcgggtat cgaagacaac cgtatgctga ttgaaatggc
     5041 cgtcgaagaa aacgacgaag aaggttttgc cgccgtgaag gaagacgtgg cggggctgga
     5101 aaaacagatg gcggatttgg agttcaaacg gatgttcaac cagcccgccg acccgaacaa
     5161 ctgctttatc gacatcaccg ccggcgcggg cggtaccgaa gcggaagact gggcggggat
     5221 gctgttccgt atgtacagcc gctatgccga gcgcaaaggc ttcaaaatcg aaatccttga
     5281 ggaagacgac ggcgaaatcg caggcatcaa ccgcgccacc atccgagtgg aaggcgaata
     5341 cgcttatggt ttgctgcgta ccgaaaccgg cgttcaccgt ctggtgcgct actcgccgtt
     5401 tgattcgaac aacaaacgcc atacctcgtt tgcctccgtg ttcgtttacc ctgaaatcga
     5461 cgattccatc gaaatcgaaa tcaaccccgc cgatttgcgc atcgacacct accgtgcatc
     5521 gggcgcgggc ggtcagcaca tcaacaaaac cgactccgcc gtgcgtatta cccacgagcc
     5581 gacggggatt gtggtgcaat gtcaaaacga ccgttcgcaa cacgccaaca aagcggccgc
     5641 gatggaaatg ttgaaatcca aactgtatga attggaaatg cgcaaacgca acgaagagaa
     5701 acaggcattg gaagaaggta aatccgatgt gggttggggc agccaaatcc gttcgtatgt
     5761 tttggattcc tcacgtatca aagacttgcg tacaggctac gaagtcggca acaccaaagc
     5821 cgtattggac ggcgacttgg acggctttat cgaagccagc ctgaaacagg gtgtgtagtt
     5881 taggctgtat gccgtctgaa acctgtaccg tttcagacgg catttttgcg tttcgtgtta
     5941 taatccgcgc cgcacacggg acgggcagac agtcgccgcg tatcgcgtaa ggcatacggg
     6001 gaggaaagtc cgggctccgc agggtagaat gccggttaac ggccgggcgc ggtaacgcga
     6061 cggaaagtgg aacagaaagc aaaaccgccg atggctgctt tggcagcaca ggcaagggtg
     6121 aaaaggtgcg gtaagggcgc accgcgcatt tggtaacaat atgcggcagg ccaaacccca
     6181 ttcggagcaa gaccaaacag aacgcaatga cgctgcccgc cgagcgttcg ggtaggttgc
     6241 ttgagcatac cggcaacggt atgcctagag gaatgactgt ccgagacaga acccggctta
     6301 ccgcctgtcc ggtgtgcatc cgacacgcaa atgccgtctg aaacctgaaa tacggttcgg
     6361 acggcatttt tatttaggct gtgtttttat attccgttga tgcagccctg atgagtcgga
     6421 caaaatgcct tttactcaat aggaccggtt tccgcccaag cggaaataaa cggctatttc
     6481 ccttgcaaac agacggaatc aggcgggtat ttaaatgaca atcggcaggc tgccttccgg
     6541 ttgggggggg aaagcctgtc ctgtcgggtg gaaacggttt ggtttcatta tgcggaaaaa
     6601 tgccgtctga agggttcaga cggtatcggc gcatcggggc aggacttagt ctgtgccttc
     6661 gagcattttg ttcaatttgg ggacgagcag gagcagcagg aagcctgtgg ctgcgccgat
     6721 gtagaacagc agccggtaga agccgatttc gtcgcccgcc tgatagcctt tttcaaacaa
     6781 tacgccgccc aaagtgaagc ctaatgaaaa ggcaaggaaa ttaagggcga ccatttgggt
     6841 tttgaataaa ggcggtgcga ttttggtgga gatggacagc gcaatcgggg aaatcatcag
     6901 ttcgcctatc gtgatggcga ggacgatcag tgcgaaaacc gcaataggca tcggcgttcc
     6961 ggaggaaata aagggaacga atcccaaaaa cgacgcgccg gtaacaaata ccgccatagc
     7021 gaatttcagc ggggttttgg gttgtttgcg ccccattttt gtccacattg ccgccatgag
     7081 tccggaaaac aggatgaccc acaggctttg catagaatct ttccaggcga cgggtacagt
     7141 aaacgaaccg atggtgcggt tgacggtttc gtcgaaatag acggttgcca cggtgtaaat
     7201 ctgaaaccag acggcccaaa acatacagat ggtcaggaaa agcgggatgt aggcgatgat
     7261 gtgccgtttg ttgtcggaac tgacgcgggg gttggtcagc aggcgggcga aataggcgat
     7321 gacggcaagg atgacggtag ataagaggat gccggagaaa ttgtcgaggt tgacaagccc
     7381 ggttttgatg gcggttgcaa gtgcggcgat gagggtgatg ccgacggcgg ccgcagtttt
     7441 gccctgtcct tttgaaagcg gatgggggac ggtggggtgg ggcaggtttt tacgtcccaa
     7501 ggaataacgc cacaagccga atgccatacc gaccgccgcc gcgccgaaac cataatggaa
     7561 accgatgttt tcttgaagca gtccggtcaa taaggggcct aagaagccgc cgatattgat
     7621 ggcgatgtag aaaatggaaa atcccgcatc gcgcagcggg cgcatttcgt cctgttcgta
     7681 taatgcgccc accatagaac tggccgtaga tttcacgccg ccgctgccca atgcgatgaa
     7741 tatcagcccg attagaaggc cgtacaggcc cggtgcggcg gcaaggacga tgtgcccgag
     7801 cattacgacg atgcccgaga ggaagagggt tttttccgca ccccatacgc ggtcggcaaa
     7861 ccacgcgccc aaaatggtgg acaggtacac gctgccgctg tatgcgccga caatgccgcc
     7921 tgcgagggtt ttgtctatgc ccaagccgcc tttgtcggcg gtgtagtaga ggtaaatcag
     7981 caggatgccc tgcattccgt aaaatgaaaa acgttcccac aattcgatat ggaagagggt
     8041 ggaaagctgg aaggggtggc cgaaaaatgt tttttctccg gtgcgggcgg gatgccggga
     8101 cataaaggtt ctcctaaaag attttctctc aataccgtct gaaacggatg cggacggcgg
     8161 cggtgttttt tgtatgaaaa tatcggtttt taaccgatat tttcattctt tgtcaaacga
     8221 cgcgctgccg tttttcgcgg gcggatgttt ttatatttgt ttcaattcaa tggattgtat
     8281 tttagaggac gtgttccgat acggcgcggg taaatccttt tctgtcaatg gcttatccga
     8341 tagggcggtt tttacttaga tggaaaatca cttccttata tatccgccac gccgaagggc
     8401 gcattatggt ggattaactt taaaccggta cggcgttgcc ccgccccggc tcaaagggaa
     8461 cgattcccta aggcgcccaa gcaccgggcg aaccggttcc gtaccatttg tactgcctgc
     8521 ggcccgccgc cttgtcctga tttttgttaa tccgctatat ttttcttaac catcccttcc
     8581 aacagccgtg cgaaggtttt tttatatcag tgggaaatgc aatattctat tgtttttatt
     8641 gtagaatttt aaaaacagat tgttgtgttc cgcgtttttg ccggtttgga agcggtgggg
     8701 cgcatcagcc ttttataaag gcatatcgga agcctgtata aggtttttga acatatcgat
     8761 cctgttccct tgcaggcgga cggcagcggc ggatggcgaa aggcgggttt tcaaatgtcc
     8821 ggtttggcgg cgcgtctttc agacggcatg gcggcggagc gtgccgcgcc gtctgaaaac
     8881 cgtttatttc cctttgccgt ttcctgcgga gtcttttttg tccgaaccga agaggcggtc
     8941 gaaacgtatg gtgtatgtca gctcgccgcc cgacgaacgg ctgccgatac gggcaaccgc
     9001 ctgtatggcg cgggtcagcc ggtaaatcag tttgacggac tgttccgcgc tggagatgcc
     9061 gtattcgtag ccgatgtaga gtttgccggt cagttgtttg ccgacggtca gcacctgttc
     9121 ggcggggttg agttcgccgg tttgcgcgtt gcggctgcgc ttgctggtaa agcccaaatc
     9181 atccaccagc ccgatgcggt cgttgatttg cccggcaagc agcgcgcctg cggctgcgga
     9241 cagggcggca ttgtcgccgc tgctgccgct gccggcacgg ttgaggatga gccaggagag
     9301 cttgtctttt tcactcatcg gttcgtttgc cgtcagcgta atgcgcgggc tgttgaggct
     9361 gcccaatatt tccacgcccg caccgacggg ggaaaggcgg cgttcggcgc ggatgttcag
     9421 gttggggtcg ttgagcgggc cgacaaagga gactgtgcct ttggtaatgt ctaaatcctg
     9481 cccgtatgct ttgtaacgcc ctttgatgac gcggaccgtg cccaccccac gcacatttcc
     9541 gcccggttgc gcggtcaggg tcagtttgcc gcctatggta acgtccgcgc cgtagccgga
     9601 gaagcggatg ccgtcattga ggtctaaagt caggttcata ttgacgggga gcgatgccgc
     9661 cgcctctttc ttgacttcgc ccaatacgac gacatcgtcg ccgacggacg gcatcgagga
     9721 tttttgcgaa ccgaacagcc cctgatcagt tttaatcata ccggtaacgg atatgccttt
     9781 ttgcggcgaa tagcgcaggc gggtgttgcc ggaaaccgtc aggcggcggt tggggcggga
     9841 caggatgcgg tatttgtcga acaccgcgcc gatatcgaca tcgggcacgc tgttttccat
     9901 gctgaccgtg ccggagagtt ccgccgtccc ttcgtgccgg aatttcaggc tgtcgattac
     9961 ccatttcctg cctgcaatat gcgaacgcag cgagccgttg tccaagatga tgccttgggt
    10021 ttggttgcgg taatagagct tgtcgccgtt gatactgccg cccaagtgcg ggtcggcgat
    10081 gctgccgccg agggttacgg cggcattcag gctgcctttg acggtttgtc cgaccggtag
    10141 gaagttgcgg aatgcttcgg catcggcaac ggtcaggttg agcctgccgc ccaaaggtgc
    10201 ggtatcgaag gagcggcttt gcccgacggt gatattgccg ttgattttcc cgtagttgct
    10261 gctaccgttg acggcggcat tgacggacgg agagcctacc cgtccgccga tttgcgcgga
    10321 ggcattcagg ctgccggtaa tgttttgcgc ggcggcgggc agaaagggct tcaatgcgcc
    10381 caagtcggga agggaggctg taatcctgcc gccgagcggt gtatttgcca tattgccgcc
    10441 gaaggcgttg ccgatgccca aatcggcgtt aatccgtccg aaacgcgcgc cgccgtcaag
    10501 caggattccg atgcggtcgt tttgaaagcg cgttttcagg gaaaatgcgt tcaaacccaa
    10561 agcctgcccg ccgggcaata cggcatcgcc gctttgccgg ctgatattga ggtagccgcg
    10621 cgcgttgtgc ccgtaggcga catcccagtc gccgtttaaa accagattgt gttcgaaggg
    10681 cggtttgaag aaattgtgca actcggcgat gtgcaggccg cgtgcgccgc cttttgccga
    10741 tatgccggtt ttcctgtccc aagaaaagtg ttgcaggttg aggctgccgc ccattgcctg
    10801 ccaatttgcc gcacttgccg ccacgtgttc cgcaccggct tcgagcgtca tacggttttg
    10861 cagcttgagg ttgaatgcgc cgccgatgtc gaggatgccg atgctgcctt tccatcgggt
    10921 aagttccctg ttgatgccgc ctgaagcgtc caaatcgagt ttgaacggtt tgccgtccag
    10981 cgtcatggcg gcgtgtgtgc ggatgcggtg ctgcgcgccc gtaccttcca gcgtcaggcc
    11041 ggcggtatcg acaaccgccg cgccgcccga cagggaaagg cggccgccct tgatatcggc
    11101 gcgcatcggg cggcttgtgc cgggtgagcc tttgagggta aaatcgagcg aacggatgtc
    11161 tgccgctttg ccgatgtgta agttgcgcgc cgtgccggaa aggtcggttt caaaggttcg
    11221 gatgccgccg tccaaatcgc cggaaaggtg tccgcgtaca tttaaagacc ccgcgagtcc
    11281 gaaaccgaaa cgggataaat cgggtgcggt gatattgagg ttaagccggt cgcctttttt
    11341 gccgaagccg ccgtctgttt tgacgatgtt ccgccccaac cgcaaatcga cggcggcacg
    11401 cggaaggtgg cgggactcgt aaacaatgtc ggcactgccg gcaatcggca cgccgttgaa
    11461 cgtaccgggc aaaaaacgca ttttgcccgt aaatttctct tttgccagtt caccggcaag
    11521 atgaatcgaa ccgttgatat tgcctgccgg aaattgcgga tcgatgcgcg aagggtcgaa
    11581 tgcgcgggaa cggatgtcca gcttgagcag gcggtcttta aacagctcga gatagccttg
    11641 cgcggtcagg ctgccttccc cggcggagat gttgacggtg tcgaacacca gtttccgctg
    11701 ttcgtttgcg gggtcgcttg cgatggcgag gctgccgtcc gtgcgtgccg tgccggtgcc
    11761 gagttgccaa gagattttgg gcgaggcggt cgtgccgccg atgccgatgc tgccgtccaa
    11821 cctgcctttg aacgcggttt gcagcacgtc ttccgcgccg acggagttga tgcctatatt
    11881 taaatcaagg atgtcttttt cggtgtcgat tttgcccgac agcctgatgc cgccccgtcc
    11941 gagcagggcg gcggacgtat tgccgatatg caccgtgccg tcctgccgga tgacaaagcc
    12001 gcccaaaacc tgacggacgg ggatgccgtt gcggtcggca aagccggctt tggtgttttc
    12061 caaatcgagc gagccttcca gtgcgatgcc gtctgaaaac gacgggatgg cggtcaggtc
    12121 gaaattcagc ccggcatcgg gcagggaagg cacgaaggcg gccgggttga tgttgaatcc
    12181 tttgaccagt acttcttcca atgttttatc caatgattcg gcaaacgggt ggatgacgga
    12241 ttttcccgag aggcggatat tgccgccgtc gatcgtcagt tcggcgcgca catccttcag
    12301 gctgccgctc agccgcgccg tactgtgtat ggtttcgcct tcgaatccgc ctttggtgta
    12361 aatggcggta tcgagggcaa acggtttttt caagccgacc gaggctgacc ccgacgaact
    12421 gctccacggc gtgtcggcgg ccttcaggtc gaggcggtgc cctttacggt cgtaacggta
    12481 tgccgcgttg aggcgttcga gatagacggt ttgtttgtca aaggttttgc ccatgctgat
    12541 tttgcccgtc tcgaagcggt cgagatagac ggcggcgggc aggtctatgc tgtcgggcag
    12601 gccttgaggc gggcgttctt ctttaggcgg agtcggtttg gttacgatgg cgatgtcgcc
    12661 ggcggagatg tcggtgatgt gcaggctgcg gcgcatcagt tcggacggtt tccacgcgaa
    12721 gcggaagcgg ctgattttaa ggtctgcccc ctcggtttct atcgaccagt tgtcgccgtc
    12781 gaagccgtcg agcagtgtgc ctttgaggtt ttgggaggaa atgtttacgc cgaaccagga
    12841 cgggatttgg tacagcccga agcgcaaacc tgcttccgta ccggcgatcc agccgaggaa
    12901 acatactgcc aaaatcagga cagacagcag tgccgccgac agcttcagca gcgggcggcg
    12961 tttttttgcc ggcggggcgg ggcggtgttc agacggcatt ttgcgcgtgc cgttttcggt
    13021 cggatcggta tctgtcggtg cggtatcggt cataatgtct gtttcaaaat ggtttgctgc
    13081 aatgccgtct gaagcggccg tatcggttta gaaacgcgtt cccaagctga tgtgccagcg
    13141 gatttttttg tcgctgtgtc cgtaggcgat gtcgaaggaa aacggcgcga gcgggctgaa
    13201 ccagcgcacg cccagtcccg aaccgtgttt cagcttcata cgtttgaaat tggcggcggc
    13261 atcgcccata tcgtggaata ccgcgccgga cagggtgcgc gtaaacggca gttggtattc
    13321 caagctgccc accaagaggg cgcgttcggg caggaccgat ccgttcggac cggcaagccc
    13381 gatgctgtca agttcgtaac cgcgcacgga agacgcgccg ccgctgcgga acatcagccc
    13441 cgaggggaca tcggcattgt cgcgtgcaac ggtgtaaccc gcttgtccgc gtatgatgaa
    13501 cgtgccgagt tttttgtttt cgggcgtgaa gaaataacct gcgcgggcag aggtgcggat
    13561 tagcgcggtg gaggacagga atgtgcccaa agtcgtcccg attttgccgt cgaggtaatg
    13621 gccgttttcg gggtgcagca cgttgttgag cagctggcgt ttccaagagg cggtcagcat
    13681 cgtggcgtgg ctgttgccca aatcgacatc cgatccgggg attttccggc cttctgcgag
    13741 aaattccgcc cccagcctgg catcgatgcc cgtgcggtcg cgcacatacc agatgccgcc
    13801 ggagaaggcg cgtttttcga ggttttgggt ggtcgaacgg ttgtaggaaa cgttgcttgt
    13861 ccagtagttg ccccgatagt tgcgcggctg gctgatgccg gcggcaagcg tggtttcgta
    13921 tttgtccata tcccagacga ccgagccgat atagcctttg ttgaagaggt tgtaatagtc
    13981 gtaggcgatt ttgccgccca aaccgtattc cgaatcgagg cggatgccgg tttcgagttt
    14041 gtggcgtttg acctcggtta cgctgacttt gacggggacg cggtcgcctt ggaggcggtc
    14101 gaagtcggct tgtacggacg cgccggaata atgtccgttt tgttcgagcg cctgttggaa
    14161 gtcgagcagc aggtcgaggt cgtagggcgt gcccggttgg aagcgcgcca ggccggagac
    14221 ggtttgttcg gggtaacgct gtgtgccggt gatttcaaag tcgccgaagg cgatggggcg
    14281 gccgctgtcc acgacgacgt tcaaatcggc ggtggcggta tcggggttga cggccgcccg
    14341 ggtgttgccg agcttggcaa gcgggtagcc tttgcgcgtt accgcgccga ggacggaagt
    14401 tttgctgttt tcccaactgt cctgatcgaa atcgctgcct accggctgct gccagttttc
    14461 cagcgcgttg cggtagtatt cggcgaggtt gccgtctgaa aggatgtcgc cgaggatggc
    14521 gacgccgacg ttggcgattt tggtgcgcgg gcccggtgtg atgtgcaccg tataagctcc
    14581 gtctttttcc gtcaggctga ccttgctgct gaaatagcct ttgctgcgga gcattgtttt
    14641 aacgttgtcc ggtgcttctt cggcaaggaa tcccgtctgt tccttatcca aaacctcttc
    14701 ctgctgctgc gtgatgagcg gcaggtgttc ttcgaccata tctttgattt cactgtcctg
    14761 cgtgtcgatg cggacgggga atttgggttt taatttgact gattcggtgt cggggctttt
    14821 gcttttgaac aatgcgaaac ccgccgcctt gttttcggaa aggtcggcgg caggcgcgta
    14881 tgcgtgcgga aagaaaaata aagccggcag gagcagggag gtcggtttga tcatcatggt
    14941 acgggtgtcg tgcatcggtg tggcggattt cgattctaac actgttcgga cggggcggga
    15001 cgcttcagac ggcataccgc cgccggtatt tcggaggttg cggcccgccg gaaatgtatc
    15061 tgcttgtttt aaggcatttt gtttccaatc cgcatatgga aaacaggctg cgtttcggaa
    15121 tagtgtcgaa aaataggcag gattaaataa atttattctc gttcaataaa ttaaactgtt
    15181 gcgccgaatg ataaaacaaa gcctgtccct gcatagggca aatcaacgca cctcaaaaca
    15241 cagcttgaaa aataaaaaaa cacagagaat gagttttctt ttaaaataca acatttttta
    15301 acatatcaca acataaggaa gaattatggc gttcggcaaa tccttatttc atgcgatcgg
    15361 ccgcgtcagc ctggtcaggc agattgccgc cggtttggcg ttgggcatcg taatcggttc
    15421 ggtttcaccg caactgggct tggcggcagg cttgttcggc agcctgtttg tcggcgcgct
    15481 caaagcggtc gcgccggttt tggtatttat tttggcggcg gccacaatcg cgcagcacca
    15541 aaaaggcaac aaggtgcata tcaggccgat tatcgtcctt tacctcatcg gcacgttttc
    15601 cgcagccctg accgccgtca tcgccggtat ggttttcccg acgcacattg ttttggcggg
    15661 cgcgggcgat gtgtccgccg cgccgccttc cggcattgtg gaagtgttga aatcgctgct
    15721 gatgaacctg gtcgccaacc cgattaacgc gattgccaat gccaactata tcggcatttt
    15781 ggcttgggct ttggttttgg gcgcggcgtt gcggaatcac ggttcggacg ttacgcggca
    15841 ggtcgttgcc gatttggcgg aagcggtttc caccgtcgtg aaatggatta tccgttttgc
    15901 ccctttgggt attttcgggc tggtgtcgtc cacaatcgcg gagacgggtt tcggcgcgct
    15961 ggcgggttat gcgaagctgc ttgcggtgct gttgggctgt atggcgttta tcgcgctggc
    16021 ggtcaatccc gccatcgtgt ggtggaaaat ccgccgcaac ccttatccgc tggtgtttac
    16081 ctgcctgcgc gaaagcggcg tgtatgcctt ctttacccgt tcttccgccg ccaatatccc
    16141 cgtgaatatg gctttggcga aaaagctggg gctgcacgaa gacacttatt cgatttccat
    16201 cccgttgggg gcgaccgtca atatgggcgg cgcggcgatt acgattaccg ttttggctat
    16261 ggcggcggcg cacacccaag gcatacaggt tgattttgcc accgcgctgc tgttgagcct
    16321 ggtggcgacg gtcagcgcgt gcggcgcgtc cggcgtggcc ggcggttcgc tgctgctgat
    16381 tccgctggcg tgcagcctgt tcggcatcag caacgatgtc gccatgcagg tggtcgccgt
    16441 cggcttcatc atcggcgtga ttcaggattc ggcggaaacg gcgttgaatt cttcaaccga
    16501 cgttttgttt accgctgccg ccgatttggg ccgacagaga aaccgtgcgg aataaaccaa
    16561 gctatgccgt ctgaagcccg tttggcgttc agacggcata tttttacaat tttatgggca
    16621 tatggcggaa acaataccga ctgccttgaa tatgtttcaa cacacaggtc gatacataaa
    16681 gcgtcgccct atgaaggaga tgatgatgtt taaacgcagt gtgattgcaa tggcttgtat
    16741 ttttcccctt tcagcctgtg ggggcggcgg tggcggatcg cccgatgtca agtcggcgga
    16801 cacgccgtca aaaccggccg cccccgttgt tgctgaaaat gccggggaag gggtgctgcc
    16861 gaaagaaaag aaagatgagg aggcagcggg cggtgcgccg caagccgata cgcaggacgc
    16921 aaccgccgga gaaggcagcc aagatatggc ggcagtttcg gcagaaaata caggcaatgg
    16981 cggtgcggca acaacggaca accccaaaaa tgaagacgcg ggggcgcaaa atgatatgcc
    17041 gcaaaatgcc gccgaatccg caaatcaaac agggaacaac caacccgccg gttcttcaga
    17101 ttccgccccc gcgtcaaacc ctgcccctgc gaatggcggt agcgattttg gaaggacgaa
    17161 cgtgggcaat tctgttgtga ttgacggacc gtcgcaaaat ataacgttga cccactgtaa
    17221 aggcgatcct tgtaatggtg ataatttatt ggatgaagaa gcaccgccaa aatcagaatt
    17281 tgaatcgtta agtgatgaag agaaaattaa gaaatataaa aaagatggag aaaagtttac
    17341 tggtttggtt gctataaaag ttgaaaataa tgggttgaat aaatatacca ttatttatca
    17401 agctcagcct actcgttctg cacggtcgag gaggtcgctt ccggccgaga ttccgctgat
    17461 tcccgtcaat caggccgata cgctgattgt ggatggggaa gcggtcagcc tgacggggca
    17521 ttccggcaat atcttcgcgc ccgaagggaa ttaccggtat ctgacttacg gggcggaaaa
    17581 attgcccggc ggatcgtatg ccctccgtgt gcaaggcgaa ccggcaaaag gcgaaatgct
    17641 tgcgggcgcg gccgtgtaca acggcgaagt gctgcatttc catatggaaa acggccgtcc
    17701 gtacccgtcc ggaggcaggt ttgccgcaaa agtcgatttc ggcagcaaat ctgtggacgg
    17761 cattatcgac agcggcgatg atttgcatat gggtacgcaa aaattcaaag ccgccatcga
    17821 tggaaacggc tttaagggga cttggacgga aaatggcggc ggggatgttt ccggaaggtt
    17881 ttacggcccg gccggcgagg aagtggcggg aaaatacagc tatcgcccga cagatgctga
    17941 aaagggcgga ttcggcgtgt ttgccggcaa aaaagatcgg gattgatgtt gatgccgccc
    18001 gaagcgcagg tattcattac ggcgggacga cgcgttcaaa tcatcaaaca agcccccgaa
    18061 aaaggcggca tcccaaaatc cggcactgaa attgccgcgc gtatcggcgg cattccgcag
    18121 cctgccgggg aaaccgtcgg cagcagccgg aatgtcgggt atttccgccc gcctttttcg
    18181 ggctgttgaa atatgaaaat aaagcatagg ccgtctgaat gttcagacgg cctgtttttt
    18241 taacggctgt gtttcaacac tccgccggtt ccaaccggca gcagcaggga gataatgcct
    18301 tgaaccccgc tcttcggggc ggcagggttc gggccggttt ggggaaaggc atcgcctttc
    18361 cgaatcgggg cggtgcaacg gggtgcgcct tatgtgccga aagggtttgc ggcaacggta
    18421 tgccgtctga agcgggtgcg ttcagacggc attgtgatgt ttggggcgcg ggtccggttc
    18481 aaatccgcat cagttgataa acccgttgtc tttcattttc ttttcagaaa aatactggcc
    18541 gcataccagc agggcggcga ggatgatgac aaacagccct gccatagtca gtgtgatttt
    18601 gaagaacaat tcggcttcga gaaaagaaaa ccaaagttgc acaatcagca gcagcaccca
    18661 gccggcaaac agtccgagac aggcataaac gccgagctta aacacggtcc cgctccaaaa
    18721 tgacgacgta ggcttcgcgt ttgaaaaaca acagcatacg gcgcaaatct ttttccatcg
    18781 ttacgacccg ccagccttcg ggggtttgtt tgttgaggaa cgcgctgaaa ttgacggggt
    18841 tgacctttgc cgcgccgaaa aacaggctgg acaacaggct ttcctgataa atgacgactt
    18901 tgtattcttt catcgtattt ccttttcggt tgaaaccccg ccctttgggg cggtaggatc
    18961 agactttatt tgggagggaa ccccttccaa atcaggacga tacatagggc ggtgctttat
    19021 gtatcgtcct gtgtgttgaa acataggatt cctttaatca agggattgcc ttttcaggca
    19081 atcccttaaa ttgcagcgat gcaatgcggc gaaatttatt gcttattgcc caactgcggc
    19141 aataccgagc cttcgcccag cgagagcaaa ccgctttgcg aataaatacc cagtttggcg
    19201 cgggtgtcgg tgatgtcgag gtttcgcatc gtgagctgtc cgatgcggtc gagcggtgtg
    19261 aacgccgcac cttcgacttt ttccatactc aggcgttcgg gttggtaggt caggttgggc
    19321 gattcggtgt tcagaatcga gtagtcgttg ccgcgccgca gttcgagggt aacttcgccg
    19381 gtaacggctt tggcgaccca gcgttgggcg gtttcgcgca acatcaaggc ttggctgtcg
    19441 aaccagcggc cttggtagag caaacgtccg aggcgcaggc cgttgatgcg gtattgttcg
    19501 atggtgtctt cgttgtggat gccggtaacc aagcgttcgt aggcgatgtg gaacaatgcc
    19561 atgcccgggg cttcgtagat gccgcgcgat ttggcttcga tgatgcggtt ttcgatttgg
    19621 tcgctcatac ccaagccgtg gcggccgccg atgcggttgg cttcgaggaa gagttcgacg
    19681 gggtcggcgt attctttgcc gttcaatgca accggcacgc cttcttcaaa gcgcacgctg
    19741 acttcttcgg gttcaatttc gacgttttcg tcccagaagg caacgcccat gatgggctta
    19801 acgattttga tgcccgagtt caaaaattcc aagtctttgg cttcgtgggt cgcgccgagc
    19861 atattggaat cggtggagta ggctttttca accgacattt tgtagttgaa gccgttggca
    19921 atcagaaatt cgctcatttc gtgacggccg ccgagttcgt cgataaattg ctggtcgagc
    19981 cagggtttgt agattttcag cgcgggattg gtgagcaagc cgtagcggta gaaacgctcg
    20041 atgtcgttgc ctttgtaggt gctgccgtcg ccccagatat tcacatcgtc ttctttcatg
    20101 gcggaaacga gcatggtgcc ggtcacggcg cggcccagag gcgtggtgtt gaaataggcg
    20161 atgccgccgg tggaaacgtg gaacgcgccg cattggatgg cggcgatgcc ttcgtgtgcc
    20221 agttgtgcgc ggcagtcgat caggcgggcg ttttccgcac cgtattccat tgcttttttg
    20281 ggaatggcgt tgtaatcgtc ttcgtcgggc tggccaaggt tggcagtgta ggcataaggc
    20341 agcgcgcctt tgagtttcat ccacaacagc gcggcagagg tatcaagacc gccggagaag
    20401 gcgatgccga ctttctgacc gacggggagg ttttgtaaaa tagtatggtt ttggttcatg
    20461 aaaaaatcct cgatagcttg aaaaatcaaa gccattatct tataaataga aaatattgtc
    20521 agccatcatg ccgtctgaac gggtattccg ccgtttcagg cggtatttaa aaccgaaaac
    20581 attatgaacg cgttcaacct catcatcatc ggagacgaaa tcttacacgg cagccgccaa
    20641 gacaagcatt tcgccttttt taaatccctg ctcgaatcca aagggctgaa gctcaatcag
    20701 gtgcaatatc tgcccgacga acccgatttg ctggtcaggc aactgcgccg cagcttttca
    20761 gacggcatac cgacgttcgt taccggcggc atcggcgcca cgcccgacga ccgcacgcgc
    20821 caaaccgcag cggctgcttt ggatttgccc gtcgtccgcc atcctgaagc cgccaagttt
    20881 atcgaaggca taacccggaa acgcggcaag ccgctcgatt cgcccgaaca cgcccaacgc
    20941 ctgaaaatgg cggatttccc cgagggcgca gagcttgtgc ccaacccgtt caacaacatc
    21001 gccggatttt ccatccgcga gcattatttc ttccccggct ttcctgtgat ggcgcacccg
    21061 atggcggaat gggttttgga aacttattat gccggccgct tcaaccaaac cgaacgcggc
    21121 agccgcagcg tgtatgtgtt tgagcagccc gaatcgcgca ttacgccgat tatagagcat
    21181 atcgagcaaa cttatcccgg cgtgcgttcg tacagcctgc ccagcgtcgg ttggacgcat
    21241 tcagacggca cgcaggtcaa accgcacatt gagttcggca tcaaagccga aggagaagcc
    21301 gtcaaccttt tggacgcggc gtgggcggaa gtgttgcaca gcttggacgg attgggagca
    21361 aagctgaaaa atcgggtaaa ctgaaaactg tttgcccaaa tcggacgata tttaaggaaa
    21421 accatgcaat accgaatcag aagagaaaac gaagcacccg aagcaaaaaa tgcgggggaa
    21481 accttatggg agcgcgacat tatgcgcgaa gtgctgctgt ccgcctatcg ggacaggcgc
    21541 agggagcgga tgtggaaaaa catctggcgc gccgtcagca ccctgattct ggttgccctg
    21601 attgcaggca ttttccgaaa agacgaagcc gcattgcagt tggcgggcaa cacgccgcat
    21661 accgcagtcg tcaacctgta cggcaaaatc ggcaacggcg tagaagacca ggtcaaaaaa
    21721 ctcaaagacg ggatggaggc cgcctacaaa aacccgcagg caaaagccat cgtcatccgc
    21781 gccaacagcc ccggcggttc gcccgtcgtc tccaacaccg cttttgaaga aatacgccgt
    21841 ctgaaggcgc agcaccccgg catccccgtt tatctcgtgg cagaggatat gtgcgcgtcc
    21901 ggctgctact acatcgcggc ggcggcggac aaaatctatg ccgacccgtc cagcatcgtc
    21961 ggcagcatcg gcgttatcgg cagcagtttc gacgcgaccg gcctgatgga aaaaatcggc
    22021 gtaaaacgca gggttaaaat tgcgggcagc aacaaaggca tgggcgatcc gttttcgccc
    22081 gaaacgcccg aacagtcgaa aatctgggaa gaaatgctga ccggcataca cggcgaattt
    22141 atcaaagccg tcaaaaccgg acgcggcggc cgcctcaaat tccgacaata tcccgacgtg
    22201 ttcagcggtc gcgtgtacac aggcgcggac gcgctgaaag tcgggctggt ggacggactc
    22261 ggcaatattt acagcgtggc gcgagacgtg gtcaaagctc ccgatgtggt ggattacact
    22321 ccgaaggacg atttcggcag aatcttgggc agacgcttcg gagcagaact caaagcaagc
    22381 gtttcggaag ctctgcaggc cgtccggtag cgttgtttac agtatttcta agggaatacc
    22441 agaataaaaa tatacacaaa aacactcacc tccccagtat gctgccacta agtcagccgc
    22501 gttactattt accgtgcact taaagccgca aaaggcaggt tgtccccggg aggttttgtg
    22561 catcctgata ccgatcggtt gctgttgctg ttgctgttgc tcaaaggacg gaaggccgcc
    22621 gataaacgag attacctgtt tgtcgctatt gacgattttt atactctgcc attttgccgg
    22681 acaaaaccgc agacagtgct gccaagtttc tgaccgaaca tctggctgac ccctgcttgt
    22741 acctggttga gtacgtttac tctgacaatg ataggtaata taaagagccg tccaacatgc
    22801 tttcggtgca gcttgttatg ataatgggat tggttggagg cttgcccgat ttgcttgtcc
    22861 gcagaccaac ggtaaggcgg agcgggttat ccgtaccttg atggagatgt ggcatgagga
    22921 acagtcgttt gacagtccgg aacatcagca aaaggagttg tgctgcttta ttaatttagg
    22981 cgctgtccta gataactagg gaaattcaaa ttaagttaga attatcccta tgagaaaagc
    23041 cgtctaagcc ggtataaaca aaataaactc attgggctat ttgtcgcagg tgtaactgca
    23101 agaacagcgg cagagttggt aggcgttaat aaaaataccg cagcctatga ttttcatcgt
    23161 ttacgatgac tgatttatca aaacggtccg catttagaaa tgtttgatgg cgaagtagaa
    23221 gcagatgaaa gttattttgg cggacaacgc aaaggtaaat gcggtcgcgg tgctgccgga
    23281 aaagtcgcag tattcaggct tctcaaacga aatggtaagg tttcctttgc cgtccgttat
    23341 gtacgtttcg taacattcga agacttcctg aaccctgccc gccgtttcgg tttccgtacc
    23401 cccctgttag ataagggggg aagattcgaa gcggttgccg gcttcctgcc gtccgccggc
    23461 gcctccgtca tcacgccggc aaccgccttt gtcatccctt gcttatctcc catgtgcgaa
    23521 tcctcaaaaa cgggcaaaaa aaaagccctg ttacttgtag aaagtaaagg gcgttaattt
    23581 ttgttaatcg ccccttctta gggacgcaat atataaggtt tatacggtta ctgtagcgaa
    23641 tactcaaacc gctactttat ttcctattat ccgcgaacaa gtgaaacctg acagcattgt
    23701 ttatacggat tgttatcgtc gctatgatgt attggatgcg ggcgaattta gccattttag
    23761 cttcgctgaa acttcgtttt cgtatcaatc acagcacaca ttttgccgaa cgacaaaacc
    23821 atattaatgg aattgggaac ttttggaacc gggcaaaacg tcatttacgc aagtttgacg
    23881 gcattcccaa agcgcatttt gagctgtatt taaaggagtg cgaacggcgt tttaacaaca
    23941 gtgagataaa agttcttgtt ccattttaaa acaattagta aaatcgagtt tatcctagtt
    24001 gtccaggacg gcctcatctc tttatagata gctgcttacc aagtatcctt aattgctaat
    24061 tcaagccact cttcaaaact attaaaaaat tcatggtttt ctatattatc taaattaata
    24121 acaataaccc taggatttcc tgttacatca ttaccatcaa aataagcttc ccattctcca
    24181 ttactagcga atgggataag atttaaatca ggcaggatgg aatttctaaa tatataagct
    24241 acttctgccc cttctgttcc ataattctca aaccaccagt gaaaattatg tggctgaata
    24301 taaggtatta aagatgtatc gttagagata cgaacatact tttcaggata acaaaaccct
    24361 tcaggaagca tattactagg atataaatta aattcatttt gaataatcat ttcgttacct
    24421 tagtatcata aatactacca ttaggaaact gggtagacca atttggtggt gctagtccat
    24481 tttcactttg tttttttaat tctaaattga atagcttctc cccatgtttg cggcatctaa
    24541 tgctgcttgc tctgcatcaa tattactaaa aattagatta gtttcaaaaa tgaaaaatta
    24601 ctgttttttc attttttata gcatattcaa gtacatgaat aatatttttc actaagggct
    24661 ctgtagaaaa atctgaattt tgtaattttt cttttacagc caaaatactt tccttatcta
    24721 ttagccaatc cccctcactt ggtccaaata accaattatc ggtaatttca gaaacaagcc
    24781 cccataatcc acgattttga tattcaataa tttgattttc cgtaatttct acaactgttt
    24841 cagattgaat gttttcataa gcccaatcaa ccatttcagt atattcacta attgaaacat
    24901 tgtcttttaa acagaaaatt aacaggttgt gttcattcat ttgtctaagt tcctgatatg
    24961 agtttgttgt tgtaaataat cttttgcttg cttaccttgt aaattaccgg ttttcacagc
    25021 attaccattt gaatcaagat actgttttcg tgaattatca tggattctaa aatagttgtt
    25081 ttcgttatct tttataattg taatttttcc atctaaacta gtgtatcgag ttttaacttt
    25141 atcaggactt aaaataccag gtgcattggg agttagtgtt ttgtgcactg aatcaaaact
    25201 tgctgaactc cagttactat taaacttctc aaaattttgt tttctttttt cagcggcgct
    25261 gtttggtaaa gatttaatag gtttattccc acctgccatc tccccatcta aagtctgcat
    25321 atggcgggca gcaggttttt tatagccggt atttttaacc caatcccatt tggtcgggtt
    25381 aagttctact tttttacctc tccaaaccgt agttgcggct tctgctacgg aaagggcagt
    25441 ttgggctgtt gcagttatat tcggatgggc atcagcccat tgtctggcgg aattgatgcc
    25501 gtcttttacc gcaaaggcac tgtcctgtaa taggctcgcg gcagcaagtt gtgcttccgg
    25561 acttaaattt cctaaattgt gaataccctg taaagttttc cgtgctgccg cataggttac
    25621 cgggtttacc gcactgtctg taattgcccc aactcccaat ccctgaaaac ccgttaaaaa
    25681 aggattaacc gcaccttgga cgatgccgcg aatatcatcc atacggttag cccaccaatt
    25741 tttatcgggg tcgttttccc atatcagttt tcctgcttca tccgcacagt tagggttggc
    25801 attgatgccc acccagtttc agacagcctg aaaaggattt ttaagatacg gctaggtggg
    25861 ctgcgagaac ccacactctg cataaccatt tagttagatg tatccattag attatatgct
    25921 tcaattaaat gttgttctag ctcttgcact aattgaggat tgcgaattcc tgacacaatc
    25981 tcaaattctt ctgcatctat gcaaacacct gcataaattt caataacaag ggaacgcaat
    26041 aattgaagct cttctcttgt taaagaaata ataatgtcat cacctgtgta attgattata
    26101 ttcataataa ttttattttt gcttgtcaaa gtaagtttta cctaaggttg gtctaaatgc
    26161 agtcccacca tcttttgaat ttgggtctct aattacaatt gctccagact tatcatccca
    26221 aattgctctt atgtgtttgg attgtaatct tcgaattccc aagaaaaaaa tcgtaataag
    26281 tttgaaagtg tcaaatccca agtttctttt gagcaatatt ctaatatttt atcaatttca
    26341 cttttaataa tcttatgatc aaactgttct aatattaatg cattagacca aaaaaaacct
    26401 tctttattac aatgatggga aatccattta ggagaacaaa tgcaaagtga aaaaatagat
    26461 gagccttgtt ctccttcgat tccgatatcc aaatctatcc atctatgaaa attatctgga
    26521 atttcggggg taaatttttc aaaatcaata tcatataaat ttatgctttt taagtccaat
    26581 ttaatcattt tatattcaaa tgtccattag ttaaggtttt tccatcaaca cttctagtaa
    26641 caaaattagc ttgcactccc gttgggttaa gtgattcagg ggtgtttgat tttgtagttg
    26701 gaggacgata tagccttgtg ccatctgcac ttaacataca tccttgacaa gatggactac
    26761 taactggctt agccccgtct ccaacccaaa tttttcctaa ctgttcagcc tcttgacgag
    26821 ttgcagaacc aataccaaaa ttaatttccc cctttttatt tggttgtatt gttttccaat
    26881 cttttacagc acttgctaac cttgaatctt gagccgcaat gttatttaag ttttgctcat
    26941 ttagctgatt aaccaactta ggataggttg cagcattagc ttttcctttc gacgttatag
    27001 atttaggcgg tctattccca ccggccatct ccccatccaa agtatgcatg gtgcgaacag
    27061 caggtgtttt atagccggta tttttaaccc aatcccattt ggccgggtta agttctactt
    27121 ttttaccgcc ccaaaccgta cctgcggcct ctgctacggc aagggcagtt tgggctgttg
    27181 cagttatatt cggatgggca tcagcccatt gtctggcgga attgatgccg tcttttaccg
    27241 caaaggcact gtcttgtaat gcggttgcag ccgcaagttg tgctgcggga cttaaatttc
    27301 ctaaatcgtg aataccctgt aaagtttgct gcgcagaaat gtaggtcgga tatttatttg
    27361 tatccgacaa aaatatttga cgcgcctatc gtttccgaga tgccgctgtt ggaaaatgtc
    27421 ggattcgaga atccgatcta cggctgctag tgggtgctcc atcaggcata ttaacaacat
    27481 caaccctgtc gccaacccat gatttttttc tttcatttcg gctacaatag aatcatagtt
    27541 gtaatttgca ctaggctctg acaaaaattt gggttgttaa gttgtcattt ttttataaat
    27601 tccagcaaac tgttgttgta aattatctac ttcatgtttt ctaacaccag atacattttc
    27661 aaattctctt gaatctacac atacgcccgc atatatctca ttcaagatag ctcgtaaaac
    27721 aaaaacttca tcttgagtta gtgaaatagt tgagttttga ttatttatgg atagtatatt
    27781 catggctttt tcctataaat catcataata ttttttacct gatgttggtc taaatgctgt
    27841 acctccatcg tcagaatttt tatctcggat aactattgtc cctgttttat catcccaata
    27901 cgcagttcta ccgcgaggta actctttcat atttgttgga tggctaacaa tattttcaat
    27961 atgccgagca aaatctgctg gtgaattgat atttaaatcc ataaattctt gttgtcttat
    28021 gacatgcttg ttataagcat gcccactact aatttgttct ccaattagtt gcgcttgtaa
    28081 agacggttgt gtggaagcat ctgctttgct gttggacgtt atagatttag gcggtctatt
    28141 cccaccggcc atttccccat ccaaagtatg catggtgcga acagcaggtg ttt
//
LOCUS       DAXFZR010000016        27802 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000016 DAXFZR010000000
VERSION     DAXFZR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27802
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.016"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            489..959
                     /locus_tag="ACFJAS_000608"
     CDS             489..959
                     /locus_tag="ACFJAS_000608"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981923.1"
                     /translation="MKYHKIYSKILFSPPLMHKLGYTLCQYAEKKTFAPTPFHPHKFQ
                     THFRLKYPQHSRSQRHRQPFGKEKRDVVRTTSKLSTKPIPHEKQSTGMHKGNPPYRHP
                     FKTAIQMILKIKKSYPQKIPHINIIIVLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            1039..2487
                     /gene="gnd"
                     /locus_tag="ACFJAS_000609"
     CDS             1039..2487
                     /gene="gnd"
                     /locus_tag="ACFJAS_000609"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC3981924.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGRLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2550..3821
                     /gene="waaA"
                     /locus_tag="ACFJAS_000610"
     CDS             2550..3821
                     /gene="waaA"
                     /locus_tag="ACFJAS_000610"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC3981925.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWVGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3862..4311
                     /locus_tag="ACFJAS_000611"
     CDS             3862..4311
                     /locus_tag="ACFJAS_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC3981926.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMVRSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4344..5312
                     /locus_tag="ACFJAS_000612"
     CDS             4344..5312
                     /locus_tag="ACFJAS_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC3981927.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKTA"
     gene            5388..5463
                     /locus_tag="ACFJAS_000613"
     tRNA            5388..5463
                     /locus_tag="ACFJAS_000613"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5421..5423,aa:Lys,seq:ttt)
     gene            5684..6937
                     /gene="murA"
                     /locus_tag="ACFJAS_000614"
     CDS             5684..6937
                     /gene="murA"
                     /locus_tag="ACFJAS_000614"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC3981928.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            7018..8211
                     /locus_tag="ACFJAS_000615"
     CDS             7018..8211
                     /locus_tag="ACFJAS_000615"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC3981929.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGEVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(8262..8510)
                     /locus_tag="ACFJAS_000616"
     CDS             complement(8262..8510)
                     /locus_tag="ACFJAS_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC3981930.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8603..9520)
                     /gene="ftsX"
                     /locus_tag="ACFJAS_000617"
     CDS             complement(8603..9520)
                     /gene="ftsX"
                     /locus_tag="ACFJAS_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC3981931.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(9517..10167)
                     /locus_tag="ACFJAS_000618"
     CDS             complement(9517..10167)
                     /locus_tag="ACFJAS_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC3981932.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10361..10843)
                     /locus_tag="ACFJAS_000619"
     CDS             complement(10361..10843)
                     /locus_tag="ACFJAS_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC3981933.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10967..11320
                     /gene="arsC"
                     /locus_tag="ACFJAS_000620"
     CDS             10967..11320
                     /gene="arsC"
                     /locus_tag="ACFJAS_000620"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC3981934.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11317..11973
                     /locus_tag="ACFJAS_000621"
     CDS             11317..11973
                     /locus_tag="ACFJAS_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC3981935.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            12089..13483
                     /gene="gltX"
                     /locus_tag="ACFJAS_000622"
     CDS             12089..13483
                     /gene="gltX"
                     /locus_tag="ACFJAS_000622"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC3981936.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILGGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            13504..13910
                     /locus_tag="ACFJAS_000623"
                     /pseudo
     CDS             13504..13910
                     /locus_tag="ACFJAS_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13914..14405
                     /locus_tag="ACFJAS_000624"
     CDS             13914..14405
                     /locus_tag="ACFJAS_000624"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3981937.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(14681..17275)
                     /gene="mutS"
                     /locus_tag="ACFJAS_000625"
     CDS             complement(14681..17275)
                     /gene="mutS"
                     /locus_tag="ACFJAS_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC3981938.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17706..18710
                     /gene="gap"
                     /locus_tag="ACFJAS_000626"
     CDS             17706..18710
                     /gene="gap"
                     /locus_tag="ACFJAS_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC3981939.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18862..19542)
                     /gene="tsaA"
                     /locus_tag="ACFJAS_000627"
     CDS             complement(18862..19542)
                     /gene="tsaA"
                     /locus_tag="ACFJAS_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC3981940.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            20002..20637
                     /locus_tag="ACFJAS_000628"
     CDS             20002..20637
                     /locus_tag="ACFJAS_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC3981941.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVDNPVDMLKPTGLSDADLTWVAHAMVGTCGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNK"
     gene            20674..21642
                     /gene="waaC"
                     /locus_tag="ACFJAS_000629"
     CDS             20674..21642
                     /gene="waaC"
                     /locus_tag="ACFJAS_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC3981942.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            21868..22617
                     /locus_tag="ACFJAS_000630"
     CDS             21868..22617
                     /locus_tag="ACFJAS_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC3981943.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22628..23563
                     /locus_tag="ACFJAS_000631"
     CDS             22628..23563
                     /locus_tag="ACFJAS_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC3981944.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            23626..24237
                     /locus_tag="ACFJAS_000632"
     CDS             23626..24237
                     /locus_tag="ACFJAS_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC3981945.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(24289..24627)
                     /locus_tag="ACFJAS_000633"
     CDS             complement(24289..24627)
                     /locus_tag="ACFJAS_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC3981946.1"
                     /translation="MKKEQANTQKRIDEIQSKYREWCHLLLQLEENIRRWKYAVALIR
                     DMDNFYTHEYQVRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSV
                     DTLDRYTQEN"
     gene            24764..26035
                     /gene="purD"
                     /locus_tag="ACFJAS_000634"
     CDS             24764..26035
                     /gene="purD"
                     /locus_tag="ACFJAS_000634"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC3981947.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            26069..26671
                     /locus_tag="ACFJAS_000635"
     CDS             26069..26671
                     /locus_tag="ACFJAS_000635"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC3981948.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPFENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            26686..27201
                     /locus_tag="ACFJAS_000636"
     CDS             26686..27201
                     /locus_tag="ACFJAS_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981949.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(27353..27793)
                     /locus_tag="ACFJAS_000637"
     CDS             complement(27353..27793)
                     /locus_tag="ACFJAS_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC3981950.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     YTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         6658 a         6389 c         7846 g         6909 t
ORIGIN      
        1 ttttattcat attttattcc ttaagtttgc cttcgcggga cggcgcgccg cgaacgccgg
       61 ggttcggtaa accgcccgat tccgcgcccg ccgaattgct gattgaaaag cttacctccc
      121 cattttaact ttgcgcactt atcacggtaa agcgtttttt agcaaaccgc cgcagatgcc
      181 cgaccggtcc ggattcccgc ctgcgcggga atgacggcat cggtttgacg gtatttaatt
      241 gaattgcgga aattgacgga ttcagtgaga ttggcgggat gaagcctacc ctacagcccg
      301 ccttttatag cggattaaca aaaaccggta cgggttgccc cgccccggct caaagggaac
      361 ggttccctaa ggcgcccaag caccgggcga accggttccg taccattcgt actgcctgcg
      421 gcccgccgcc ttgtcctgat ttttgttaat ccgctatgct tatacacaac cagccaaaag
      481 ccatcttatt gaaatatcat aagatttact caaaaatctt attttctcca ccacttatgc
      541 acaaactcgg atacacactc tgccaatatg cagaaaaaaa gacattcgcg ccgacacctt
      601 ttcatcccca caagttccaa acacatttcc ggcttaaata tccacagcat tcccgttctc
      661 aaaggcaccg gcagccattc ggtaaagaaa agcgggatgt cgtccgcaca acaagcaaac
      721 tatccacaaa acctattccc catgaaaaac aatccacagg tatgcacaaa gggaatcctc
      781 cttaccgaca cccttttaaa acggcaattc agatgatttt aaaaataaaa aaaagttacc
      841 cacagaaaat tccccatatc aatataatca tcgttttatt atttaaacct tatattttat
      901 ttataaccct tcagggaaac ggcacggcaa agcgtaaagg catcttttca aaaaattaaa
      961 aagttaagga ttgttaaaca agtgtaaatc aggtagagtt gcagccgata aattaaatct
     1021 ataacggagc ttcctgcaat gaaaggcgat attggcgtaa tcggtttggc ggttatgggt
     1081 caaaacctga ttttgaatat gaacgattgc ggatttaagg ttgtcgccta caaccggaca
     1141 atcggtaaag tggacgaatt tttaaacggt gcggcaaaag agacgggcat tatcggcgcg
     1201 tattccctgc aagacttggt cgataagctg gcaaaaccga gaaaaatcat gatgatggtt
     1261 cgcgcaggtt cggtggttga cgactttgtc gaacaactgc ttccgctgtt ggaagaaggc
     1321 gacatcatta tcgacggcgg caatgccaat taccccgaca cgacacggcg gacacattac
     1381 cttgccggaa aagggatttt gtttgtcggc gcgggcgtat ccggcggcga agaaggcgcg
     1441 cggcgcgggc cgtccatcat gccgggcggg gataagcgcg cttgggaagc ggtcaagccg
     1501 attttccagg cgattgccgc caaaacgccg cagggcgaac cgtgttgcga ctgggtcggc
     1561 aaggatggcg cgggacattt tgtcaaaatg gtgcacaacg gcatcgaata cggcgatatg
     1621 cagctgattt gcgaagcgta ccagtttatg aaagacggtt tggggctgtc ctacgacgaa
     1681 atgcaccgcg tgtttgccga gtggaacaaa accgagctgg attcttatct gattgaaatc
     1741 acagcggcaa ttttgggcta taaggacgaa ggcggcgaac cgctggccga gaaaatcctc
     1801 gatacggcag ggcaaaaagg cacgggcaaa tggacgggca tcaatgcctt ggatttgggc
     1861 attccgctga cgctgatttc cgaagccgta ttcgcccgct gtgtttcgtc gttcaaagaa
     1921 cagcgcgtgc agaccggcag gctgttcgcc cgaaccgcca cgcccgtcga aggcggcaaa
     1981 caagaatggg tcgaggcgtt gagacaggct ttgcttgcct ccaaaatcat ttcctacgca
     2041 caaggcttta tgttgatccg cgaagcgggc gaaagttacg gctgggattt ggactacggc
     2101 aacactgcgc tgctgtggcg cgaaggctgc atcatccgca gcgcgttttt gggcaatatc
     2161 cgcgacgcgt atgaggccaa tcccgatttg gtgttcttgg gtgcggatcc gtatttcaaa
     2221 aatattttgg aaaactgcct gccggcatgg cgcaaggtgg ttgccaaggc ggtcgaatgc
     2281 ggcatcccta tgccctgcat ggcttcggcg attacgttct tggacggcta tacgaccgaa
     2341 cgcctgcctg ccaacctctt gcaggcacag cgcgactact tcggcgcgca cacttacgag
     2401 cgcaccgaca aaccgcgcgg cgaatttttc cataccaact ggacgggcaa gggcggagat
     2461 accgcttcga caacctacga tatttgattc cgttgccgaa atgccgtccg aaccgttcgg
     2521 acggcatttg ttttgcagga aatagaaaga tgttccaatg gctttatgat gtattgtggc
     2581 tgcttgcgcc gatatggata cggcgttatt tggacaaacg ctccggaagt gccccggcat
     2641 accgggcgca tagggacgag cgtttcggca agccgcatcc gaatcccgtt accggcgcgg
     2701 tttggataca cgccgtttcg gtcggagaaa cgcgtgccgc ccagcccttg atacgcgagt
     2761 tgcggcggcg ttttcccgat gcgccgctgc tgatgacgca gatgaccccg acgggacggg
     2821 aaaccgcgca agttctgttt cccgatgcgc aatgccgcta tcttccgtat gacaaaaaaa
     2881 cgtgggtacg gcagtttttg cgcgaacacc gcccgatgtt cggcattttg atggaaaccg
     2941 aaatctggcc caatctgatg aaggaatgcc ggcgagcggg tgtgccgctg tttttggcga
     3001 atgcgcggct gtcggaaaaa tcgctgaacg gttatctgaa agtccgccgc ctgatccgtc
     3061 ccgcagccgc ttcgctgacg ggctgtctgg cgcagacgga ggcggatgct gcgcggttgg
     3121 cgaaattggg cgcggcatcc gtgcaggtgt gcggcaatac caaatacgac ctgatgccgt
     3181 ctgaagacat gaaaacgctg gcggggcagt ttgaaaaacg tatcggaggc cggccggttg
     3241 ccgtgtgcgg cagcacgcgc gtttatcggg gcgaagacga ggcggaaaaa ctgctggcgg
     3301 cgtggcaaca atatcgcggc gatgccctgc tggccgtcgt gccgcgccat cccgagcatt
     3361 ttcagacgac atttgaaacg gcgaaacgct tcgggtttaa ggttcagcgg cgcagcgacg
     3421 gtttgccggt cgaacccgat acgcaggtgt gggtaggcga cagtatgggc gagctgtatg
     3481 cgtattacct gtgcgccgat gtcgcttttg tcggcggcag tctggtcggt tcgggctgtc
     3541 agaacatcat cgaaccgctt tcctgcggcg ttacgacgat attcggcttt tcgacctaca
     3601 atttttccga agcctgccgg cacgccttgg catcgggtgc ggcggttcaa gtcgaatcgg
     3661 cggacgcgtg gcgtgaagcc gttgaaaaaa ccttatctgg cgaggggggc ggaatgcaga
     3721 tgcaggcgcg cgtggacggc tttatcgcac aacatcgcgg agcgggcgcg agaatcgccg
     3781 aggcggtgcg ggaagcggta tgcggacatc gggggcgata gtgatacaat ccgtatccga
     3841 gttttccggt tggagtaccg tatgagtatt tatgccgtcg cgcacatcat ccacctgtat
     3901 tgcgccaccg cctttgtcgg cggcgtgttt tttgaagtgc tggttttgtc cgtcctgcat
     3961 acgggacggg tgtcgcgcga ggcgcggcgc gaagtggaaa aggcaatgtc ttaccgcgcc
     4021 gtcagggtga tgccgtttgc ggtcggactg ctgttcgcca gcggcatcgt gatggcggca
     4081 aaccgctacc ttcctatatt gggcgaaccg tttgccactt ccttcggcac aatgctgacg
     4141 ctgaaaatcc tgttggcgtt cagcgtgttg gcgcacttcg ccatcgccgt cgtcaaaatg
     4201 gtgcgttcca cgctgacggt cggctggtcg aaatacatac acgccgtcgt ctttacccat
     4261 atgctgctga ttgtcttttt ggcaaaagcg atgttttata tcagctggta acacaactac
     4321 atttcaaatc aggagattga accatgacag aatatcccgg catcgggtcg ccgttgtttt
     4381 acggcgtttt ttttgcggca gtgctggtca tgattgcctt ggatatgttt tcgctgaaga
     4441 aaaacggcag ccataaagtc ggcatcaaag aaaccttggc atggagcggc ttgtgggttg
     4501 ccgtatcctg cctgttcgca ggctggctgt atttcgaact cgccggcaat cccggctacg
     4561 gcgcggcagc cgcaaaagaa aaagtattgg aattctttac cggctacatt ttggaaaaat
     4621 cgttggcagt cgacaatatt ttcgtgttcc tgatgatatt cggctacttc aaagtcgcac
     4681 cgcagtttca acaccgcgtg ctgctgtacg gcgtattggg cgcactggta ttgcgcaccg
     4741 tcatgatttt cgtcggcgcg gcactggttc ggcagttcga gtggattctg tatctgttcg
     4801 gcgcgttcct gctctatacc ggcatacaca tgatgaagcc cgaaggagat gaggaagagg
     4861 atttggcaaa cagcaggctg ctgaatgccg tcaagaaagt cgttccggtc ggcacggcgt
     4921 ttcacggcga gaaatttttt accgtcgaga atggcaaaaa aatcgccacg ccgctgtttt
     4981 tggtgctgat tatgattgaa ttgagcgatg tcgtttttgc cgtggacagt atccccgccg
     5041 tctttgccgt taccaccgat ccgtttatcg tgctgacttc caatattttc gctattttgg
     5101 gtttgcgggc gatgtatttc ctgctggcgg atgtggcgga acgctttatc ttcctgaaat
     5161 acggcttggc attcgtgttg ggttttatcg gtgtgaaaat gctggtgatg cattgggtac
     5221 acatcccgat ttccgtttcg ctgtcggtcg tgttcggcgc gttgggtgca tcgatactga
     5281 cctcgttaat ttatacgaaa aaaacagcct gataaataaa acgccgtctg aacgccgaca
     5341 aaatggttga cagcatcagg cttgatgtat aaactttcaa atttttcggg tcgttagctc
     5401 agtcggtaga gcagcggact tttaatccgt tggtcgagcg ttcgaatcgc tcacgaccca
     5461 ccaggtaaag caaaaaagct gagttttcaa aactcggctt ttttttgttt tccgacaatc
     5521 cttgttcgga aagcgcggca tttcgtcaaa ttctttggtg cggcttccgt atttttcccg
     5581 aaaatccctt ataattccgc ctctgcaacc ttcatcgggt gatgctttat gcattgtccc
     5641 gagtattgaa atattagcaa gtacccacca ggagggaaca aacgtggaca aactgaaaat
     5701 ctccgcaaac ggcccgctca acggggaaat aacggtctcg ggcgcgaaaa acgcggcatt
     5761 gccgctgatg tgcgcgggtt tgctgacgtc gggtacgttg cgcctgaaaa acgtccctat
     5821 gctggcagat gtcgcaacca cgcaaaagct gcttcagggc atgggcgcgc gcgttctgac
     5881 cgacaatatc agcgaatttg aaatcaacgg cggtacggtc aacaatacct gcgcccctta
     5941 cgaattggtt cggacgatgc gcgcttcgat tttggtgctt ggcccgacgc tggcgcgttt
     6001 cggcgaggcg caagtcagcc tgccgggcgg ctgcgccatc ggttcgcgcc ccgtcaatca
     6061 gcatttgaaa ggcttggaag cgatgggggc ggaaatcgcc atcgaacacg gttacgtcaa
     6121 agccaaaggc aaactcaaag gtgcgcgcgt ggcgatggat gtcgttaccg tcggcggtac
     6181 ggaaaacctg ctgatggcgg cgacgctggc ggaaggtacg acggttttgg aaaactgcgc
     6241 catcgagcct gaagtggtcg atttggcgga atgcctggtc aaaatgggcg cgaaaatcag
     6301 cggcatcgga acatcgacca tgattgtgga aggtgcgggc gagctgtacg gctgcgaaca
     6361 cagcgtcgtc cccgaccgga ttgaagcggg gacgttcctg tgtgcggtgg cgataaccgg
     6421 cggcagggtg gttttgcgga atgccgcccc gaaaacgatg gaagtggtgt tggacaaact
     6481 ggtcgaggca ggtgcggtga ttgaagcggg cgacgattgg atcgccatcg atatgcggca
     6541 gcgtccgaag gcggtggaca tccgcacggt cgtccatccc ggcttcccca cggatatgca
     6601 ggcgcagttt atggcattga atgccgtggc agagggaagc tgccgcgtgg tggaaacgat
     6661 ttttgaaaac cgctttatgc acgttccgga gttgaaccgg atgggggcga acattacaac
     6721 cgagggcaat acggcatttg tgcagggtgt ggaacggctt tccggcgcgg tcgtcaaggc
     6781 gacggatttg cgcgcgtccg ccagcctcgt tatcgccggt ttggcggcgc gcggcgaaac
     6841 cgtggtcgaa cggatttacc acttggatcg cggttatgaa aatattgaaa aaaaactcgg
     6901 cagcgtcggt gcgaacatcg agcgcgtatc cggctgatcg ggttgcgatg ccgtctgaag
     6961 gcttcaggcg gcatcaattg tttctgtatc gcaaacactg attgaaaagg actgattatg
     7021 gcatttttaa aactgaccga acaaaacgtg cggggcaaaa ccgtcctcat ccgcgccgat
     7081 atgaacgtgc cgttcaaagg cggcaaaatc agcgacgaca cccgtatccg cgcctcgctc
     7141 gcgtccgtca aatactgctt ggacaacggc gcgtccgtta tcgtgatgac ccatttgggc
     7201 cgcccgaccg aaggcgagtt ccaccccgaa gacgatgtcg cccccgttgc cgcgcacttg
     7261 ggcggcctgt tgggcaaaga cgtgaaagta ttgaacgact ggcgcgaaaa caaacccgcc
     7321 ctaaacgcgg gcgaagtcgt gatgctgcaa aacgtgcgta tcaacaaagg cgagaagaaa
     7381 aacgatttag aactgggcaa agcctacgcc gccttgtgcg acgtgttcgt caatgatgcg
     7441 ttcggcaccg cacaccgcgc ccaagcttcg accgaagccg ttgcccaagc cgcgcccgtc
     7501 gcctgcgccg gcgtattgat ggcgggcgaa ctcgacgctt tgggcaaagc cctgaaacag
     7561 cccgcgcgtc cgatggtggc gattgttgcc ggcagcaaag tgtccaccaa gctgaccatc
     7621 ctcgaatcgt tggcggacaa agtcgatcag cttatcgtcg gcggcggcat cgccaacaca
     7681 ttcctgttgg cggaaggcaa agccatcggt aaatcgctgg cggaacatga tttggtggaa
     7741 gaatccaaaa aaattatggc gaaaatggcg gccaaaggcg gttctgtgcc gctgccgacc
     7801 gatgtcgtcg ttgccaaagc ctttgccgcc gatgccgaag cggtggtaaa agacatcgcc
     7861 gacgttgccg aagacgagat gattttggac atcggcccga aatccgccgc cgcgcttgcc
     7921 gatttgctta aagccgccgg tacggtcgtt tggaacgggc cggtcggcgt gttcgagttc
     7981 gatcaattcg caggcggcac gaaagccctt gccgaagcca tcgcccaaag caaagcgttc
     8041 tcgattgcgg gcggcggcga cacgctggcg gcgattgcca aattcggcgt taccgaccaa
     8101 atcggctaca tctccaccgg cggcggcgcg ttccttgagt tcttggaagg caaagagctg
     8161 cctgccgtag ccgctttgga aaaaacgcgg cgagtaaacg gtttgatata aaaaatgccg
     8221 tctgaaacga aatggtttca gacggcattt ttgtgtggcg gttattgtgc tttggcagcc
     8281 cattccgccg gagtggcggc aaccgaaatg gaaagtgcgt gcagttcgtt gctttccagt
     8341 tgggctttga gtccgtcttt aatcaggcgg tggcgcgcga ggcgtgcctt gccttcaaat
     8401 tctgatgaaa cgatgacggc gaaaaaatgg tgtccgtcgc cttctacttc gatgtgttcg
     8461 cattttgcca cgcctgcaat cagggccttg acttgttcgg gtgtcagcat agtggggagt
     8521 ccttattggg gaaatgcgcc attatactgg caaatcgaaa ttccgtctga aaacggcttc
     8581 agacggaatt tttgacggtg ttttattttt tggctttgaa gcagagcagg tgttgtgtgg
     8641 tggcaagcca cgcgccgaat acgcccaacg cgatgacgaa gccgaacacc agcccgagtt
     8701 cgccgacgta gaagaaacgc cagccgatgt tcaagccgta gggtttgaag atggcatcga
     8761 ccaaggggcg cacggcagag agcagccaac cgcaaagccc taagctgacg gcggcggaga
     8821 agatgctctg ccacatagcc tgataaagga aggggcggcg gataaacgac gcgggcgcac
     8881 ccaggagttt ggtgatttcg atttcttctt tgcggctcag gatttgcagt cggatggtgt
     8941 tgtgtgcgac aaggacgaaa gccataccca gcgtcaggga aagaaaccat aagattttgc
     9001 ggatgaactc gttgatttgg tacagcgttt gcacccattc ggtatccata gacgcggatt
     9061 cgaccatagg cagcttggta atgtcccggt agattgcctg catttgggcg ggcgtggttg
     9121 ccgggtcggg ggtaacgata aagacatccg gcagggggtt gccgtcaagc atggaaatca
     9181 gattttggtc gaggttggac tgtaattccg ccaaaccgtc ttccttgccg atgaagcgga
     9241 tgttgtcgag ccgtttatcg cgcgtcagca ggctgcgtac ggtatcgctg tcgctttgtg
     9301 cggcggcggt ttccatatag acggtgattt gcggcgactc gttgagtttg cccaacacgc
     9361 tttgcccgct ttggatgccc agatacataa acagcggcag ggtcatcgcg acggcaagca
     9421 ttatgagggt aagcagtgtg ccgaagggtt ggcgcagaag ctgtttgagt gcggagcgcg
     9481 cggattcgac gtgcagcgag aagtagtgga tgatgctcat gcgagtcgtc ctttcgagag
     9541 gcgcaggatg cggtgtccgt agtccgccat cagggtttcg tcatgtgcgg cgacgatgac
     9601 ggtggttccc gcttcgtgga aggttttgaa caattccata atatcgagcg cgtaggcacg
     9661 gtcgaggttg gcggagggtt cgtcggcaat cagcaggccg ggctggtgga cgacggcgcg
     9721 ggcgatgcac aggcgttgtt gttcaccgcc cgagagggtt acgggatcgt ccaattctcg
     9781 tcctttcaag ccgacttttt cgatggcgat gcgggcgcgt tcttcggctt tgcgcggcgg
     9841 atagccgata atccgcaggg gcaggatgac gttttgcagg acattgcggt cgttgaggat
     9901 tttgtggtct tggaacacga tgccgatgtg ttggcgcata aagccgattt ggttgtcgga
     9961 caatgtgccg aggtcctgcc cgttaaacag gactttgccc atgctcggct tggtgatgcc
    10021 cgaaatcagc ttgaggacgg tggatttgcc cgaaccggag tgtcccgcga taaaaatcat
    10081 ctcgcctttg ttgatttgga agctgacgtt tttcagggct tcaaaaccgc cgggataggt
    10141 tttggaaact tgttcgaaac ggatcatagg aggtcctgta aggaagggcg gcatatcgtt
    10201 tcgggcggtt gcgtccgcag ggatatgccg tctgaacaat aggtaagaca gtaccgatta
    10261 taaagattcc gcctgttttt agatagtgga taagtttggc ggataccgaa ggccgggcgg
    10321 gaaaatgccg tctgaagcgg cttcagacag catccggcgt ttaacggcat tttgaatggg
    10381 cgagtttgac ggcttcggtc aggcttttct cgttcacctc tcctgtaatg gtctgcctgt
    10441 atccgcattt cggggcttcg acgacggtaa agggcagtac gccgacattg tttccgtagg
    10501 atttcataaa gtttcggctg ttcgccccgg tgtaacgcca aatcgggtag ctgaccgggg
    10561 tctgcttgag aaagttgccg atattgtcgg atgtgtcgag cgcgatgccg accatatcga
    10621 cgctgccttt tttctgcgct ttgtaccatt tggacatggc gggcatctct tttcgacacg
    10681 gaccgcacca agtcgcccat aggttggcga tgcggacggg ggctttgagc gattgcaggt
    10741 tttgcggggt attgtctttc catccggcca gttcgtctgc cgaggcggta tgtgcggcaa
    10801 atgtggcggc aagggcgatt gcggcgagat tcaagcgttt catcgttttg tcctttgtat
    10861 gtcgattcgg gtattgtaaa ccgtttggta cggttggagg cggggtattg agtaaaaccc
    10921 tgcgtaagcc gataataagg ggattgtcga gaagtaggga aatgtcatgt ctgaaatcaa
    10981 aattttccac aatccgcgtt gcagcaaatc gcgtgccgcc gtgtccctat tggaagaacg
    11041 cggcattgct gccgaagtgg tcaaatattt ggatacgccg cccgacttgt ccgaattgaa
    11101 ggatattttc aacaaattgg gcttggaatc ggcgcgcggg atgatgcgcg tgaaagatga
    11161 tttgtacaag gaattgggtt tggacaaccc cgatttggac aatgacgcgc tgctgcgtgc
    11221 catcgccgat catcccgccc tgttggagcg tccgattgtt ttggcaaacg gtaaggcggc
    11281 tgtcggcaga ccgttggaaa atatcgaagc ggtattgtga acaaacggct gttttgcagc
    11341 cgcaacggct tgcgctatta cctgttgggc ggtttctgtt tgagtgtttt tccgcttttg
    11401 ctggtgtttg cctcatccgt ttgggcggtt tatcggacgg gcgggcaggt tttgccgccg
    11461 tatgtccggg cggatgccgc gctggtgttg ggcgcagccg cgtgggacaa acgcccttcc
    11521 ccggtttttc gggaacgcat caaccacgcc atcgcgcttt accaaagccg cagggtaggc
    11581 aaaatcgtgt ttacgggagg gcgaaccaaa aaaggctata tgaccgaagc cgaggtcggg
    11641 cggcggtacg cgctcaaaca gggggttccg gcgcgcaaca tcctgtttga aaacacttcg
    11701 cgcaacactt atgaaaacct gaacaacatc cgtccggttt tgcgtgccaa cggtattgcc
    11761 agcgtggtga ttgtcagcga tccctaccat ttggcgcggg cggcggaaat ggcggaggat
    11821 ttgggtgtcc gcgtttatat gtctgccacg ccgaccacgc ggtttgatgc gggaaataag
    11881 aaaaaaatct ttatgctgca agaggggtat gcgttatccc tctaccgttt ggaaaaatgg
    11941 ggcagccgct ttttgggatg gctgtctgat tgaggcggta gaaaatgccg tctgaaactt
    12001 atccgcaggc agggttcgga cggcattttt gatgcctaag cacggtaaaa acagtaaaat
    12061 aaacgctttc aaagatttcg gagccattat gaccgtcaaa acccgtttcg cccccagccc
    12121 caccggttac ctgcacatcg gcggcgttcg caccgccttg ttttcgtggg cgtttgcccg
    12181 ccatcataaa ggcgaatttt tattgcgtat cgaagacacc gacttggcac gttccaccgc
    12241 cgaatccgtc aacatcatcc ttggcggcat gaaatgggtc ggtctcgatt acgacaacgc
    12301 cgacaacgtg gtgtaccaaa cccgccgttt cgaccgttat aaagaagtca ttgccgaact
    12361 cttggcaaaa ggcgatgcct actactgcta ttgcagcaaa gaagaattgg aagcgatgcg
    12421 cgagaaagcc gaaaaagaag gcacggcgac ttacgaccgc cgctggcggc cggaagcagg
    12481 caaaaccctg cccgaaattc ctgcaggcgt gcaacccgtc gtccgcttca aaacgccttt
    12541 ggacggcgtt accaaatgga cagacttggt caaaggcgaa atctccatcc ccaacgaagc
    12601 cctagacgac ctgattattg cccgcgccga cggtacgccg acctacaact tctgcgccgt
    12661 ggtagacgac tacgacatgg gcgttaccca cattatccgc ggggacgacc atgtgaacaa
    12721 caccccgaaa caaatcaaca tcttgaaagc catcggcgcg aacctgcccg aatacgggca
    12781 tctgccgatg attctcaacg aacaaggcaa aaaaatctcc aaacgcagcg gcgataccgt
    12841 cgccattacc gatttcggcg caatgggcat tctgcccgaa gcgatgctca actatttggc
    12901 gcgtttgggc tgggcgcacg gcgacgatga gtttttcacg acggaacaat tcatcgaatg
    12961 gtttgatttg aaagacgttt ccccgtcccc aagccgtatg gatttgaaaa agctctattg
    13021 gatcaacggc gaacacatca aaatcacacc cgacggcaaa cttaccgaac tcgtcaaacc
    13081 gcgccttgcg ctgcgcgata ttcatgaaac cgaaaaacct gcgttggaag acgtgttggc
    13141 actggtcaaa gaccgcgccc aagatttgaa cgccttggca gacgaatgcc tgtatttcta
    13201 caaaaaacaa gtccctgccg aagccgatgt tgccaaacac tgggacgacg aagccgccgc
    13261 ccgtatgctg cgcttcgccg aacgcctcga agggctggaa gactggaacg ccaaagccat
    13321 tcacgacctc ttcaaacctt tctgcgacga agaaggcatc aaaatgggca aactcggtat
    13381 gcccctgcgc cttgctgtct gcggtacggc aaaaaccccg agcgtcgatg ccgtgttggc
    13441 attaatcagc aaagaggaag tgttgaaacg catccgtgct taagttatcc acaggtgcgg
    13501 gcaatgccgt ctgatgtcgg aatgcggctt cagacggcat tcagacggca ttcagacgga
    13561 agttgaacat gaaaaaaata ttgtattttc tgatgtttgt tttttctaca agcgtatggg
    13621 cagggggtgc ggaagacaat ttgctcagca tccaatccgg ttaccgtgct ttactgcaaa
    13681 agcaaaacaa tctggacgga aaaatcatcg ggatgcagtc ggatttggaa gatgcgcgcc
    13741 ggcgtttgca ggcggctcag gcagacatcg cccgtttgga agcggaaatt cctgcagcaa
    13801 tggcccaaaa agcccggcag gctgaagaga tgaggcaaat cggagtgcgt ttggaccatg
    13861 cttggaatgc ggtttacggc gcaggggaac gaaagcgtcg ggggaattga ccgatgaatt
    13921 ccctctttgt ggacaacact gtttttatta cacggctgaa agccgggcat atcggcaggt
    13981 tggttcaggc gttgtttgag gagtggcacg gatttgaacc gtggtcttct gtggataaga
    14041 ttcgtgccta ttacggcaga tgtttgaagg atgacgaact gccgctggct tttgcggctg
    14101 tggatgattc cggaaccctg ttgggttcgg ctgcagtcaa gcggcatgat atggaaaatt
    14161 ttccacagta tgaatattgg ttgggcgatg tctttgtttt acctgaatat cgcggaaaag
    14221 gcattggcag gaggctggtc gcccactgca taggcgcagc gcgttcgctg gggataaagt
    14281 tcttgtatct ttatacgcct gatgtgcaaa tattttatga atcattcggc tgggcggttg
    14341 tcgggcgaca tttccataac ggggaatggg ttacggttat gcgtttggat gtggataagg
    14401 tttaagccga atgggcagat gccgtctgag ctgttcggac ggcatttttt atgtttggaa
    14461 atttcctgtc agatatagtg gattaacaaa aaccggtacg gcgttgcccc gccccggctc
    14521 aaagggaacg gttccctaag gcgcccaagc accgggcgaa ccggttccgt accatttgta
    14581 ctgcccgcgg cccgccgcct tgtcctgatt tttgttaatc cgccataaag tttatccaca
    14641 gatttttcca tatggtttga tgctgctccg ccaacggaaa ttaagatacg gatttgcaca
    14701 aatctttcag acggtacagt tctgacaatg cttcgcgcgg ggtcaggctg tcgggatcga
    14761 ggttttccag ggctgctgcc aatatacctt caaaatgttt ttcctctgct ttatccacaa
    14821 agcagtccac attcggttca tctccttttt cagacggcat ggtactgaaa atatccagtt
    14881 ggggacggtt tgcggcggct tggttttcca gtccgttcaa atgcttttgg gcggctttca
    14941 atgcgcgcac aggcaggccg gcgagtttgg cgacggcgat gccgtagctt tttccggcgg
    15001 gaccgggttg gatttggtgc aggaacacga tgtcccgtcc ctgttcgagc gcggaaaggt
    15061 gcatattgac ggcggcggcg tgggcttcgg gcaggtaggt cagctcgaaa tagtgggtgg
    15121 caaacaggct gaaggatttg tttttttgca gcaggtgttc ggcgattgcg tgcgcgaggg
    15181 cgaggccgtc gaaagtggaa gtaccacgtc cgacttcgtc cattaaaaca atgctttgtt
    15241 cggtggcgtg atgcaggatg taggcggttt cgctcatttc gaccatgaag gtggagcggt
    15301 tggaggcgag gtcgtccgat gcgccgatgc gggtgaagat ttgatcgaca ggcccgattg
    15361 tggcggcatc ggcaggcacg aaacagccgg tgtgtgccaa taaaacaatc agcgcgactt
    15421 ggcgcatata ggtggatttg ccgcccatat tggggccggt gagcagcatg aggcggtgtt
    15481 tgtggtcgag gtcggtgtgg ttggctgtga agtggcgtac ctgctgttcg acaacgggat
    15541 ggcggccgtt ttcgatgtgg acaaccggat agtcggcaaa ctcgggacgg acaaagttcc
    15601 gctcttttgc caaggctgaa aatgtggaca acacgtccag cgcggcggcg gctttggcgg
    15661 ctttttgaag ctgcggcaat gccgtccgaa ggtttttcaa tacgccgtca aagagttgtt
    15721 tttctaaggc gagggcttgg tcttgagcag tcagcacttt gtcttcaaag gctttcagtt
    15781 ccggcgtgat gaagcgttcg gcgtttttga gggtttgccg gcgttggtag tcggcaggtg
    15841 cttgttcggc ttgggttttg gacaattcaa tgtaaaagcc gtgaacgcgg ttgaactcga
    15901 ctttgagtgt ggacaaaccg gtacgttcgc gttccttggc ttccaaatcc agcaaaaatt
    15961 cgtcgccatg gttttgaatg cggcgcaatt cgtccagttc gggatgaaaa ccgtggttga
    16021 tgacattgcc gtctttcagc cagacggaag gttcgggcat cacggcggct ttgagggttt
    16081 cggcaactgg cagggtttcg gggaaaacgg ctttaagggt ttccaacagg ctgctgccgg
    16141 ttgcggacaa gtcgatttgt gccaactcga acagactgtc gcgtaaagat gccaaatcgc
    16201 gcgggcgggc attgccgacg gcgatgcggg cggcgatacg ttcgatgtcg gcaatgctct
    16261 tcagatggca ttggaggggt tcgtattggc tttccagcgc ggttacggct tcttggcgcg
    16321 ctcggatgtg ggcgcggttg cgtaaagggt ggtgaagcca caatgccaac aggcggctgc
    16381 ccatgtgggt ggcgcagccg tcgagtatgg aaaacagggt cggcgttttt ttaccggaga
    16441 gggtttgcgt gatttcgaga ttgcggcgcg tggcggcatc catgccgata tattggctgt
    16501 cggtttcgag cgacaggccg tccaaatgtt gcggcatcag gttttgcgtc aggcggatat
    16561 agttcaacag cgcacctgcc gcgccaatcg aaacggcgtg ttctttgctg tccaaaccga
    16621 agccgcgcaa atcttggcag ccgaaatatt ccgtcagcag tttttccccc gcgtcggcgg
    16681 caaactgcca cgcgttcagg cgtgtaacac ccgatgccgt ctgaagttgc ggtgcgtttt
    16741 tactgtccgg cagcaaaatt tccgccgcct gcaggcgcgc cagttcgtcg ttcaatttat
    16801 ccgcagttgt cagcttggtt ttgaattcgc cgctttgcag cgatgcccac gccaaaccga
    16861 tgtatttttt gtcgggagac acggcaacga tgcggttggt ttccttgtct tccagtaatg
    16921 cggaatcggt tagcgtgccg ggcgttacga tgcgcacgac tttgcgctcc accggccctt
    16981 tgcccgcgcc gacttcgccg acctgttcgc aaatcgccac gcttttgccc aacttgacca
    17041 ggcgcgccag atattgttcc gccgcgtgaa acggaacgcc cgccatttta atcggcacgc
    17101 cgtccatctg cccgcgtgtg gtcagggtga tgtccaacaa tttcgccgct tccaccgcgt
    17161 catccaaaaa cagctcgtaa aaatcaccca tacggtaaaa cactagtttg tcggtatgtt
    17221 gcgcttttat gccgaggtat tgctgcatca ttggggaaac ggcggatttg gacatggggc
    17281 tgccttatta ttaggaaaac ggtattgtag caaaaggttt tgtcttcgac ggaatgagac
    17341 tgaccgtaag cttgtcaata aagttgtgga gacaaatagg aaaacggcaa agttatccat
    17401 aagaatatta ttaagtcaaa aaacatccac gcttatctgt cttatacggc aagccttaaa
    17461 ctcaaattta tttaacaaaa aggtaatgaa aatattggat atttgctact tatacacaag
    17521 caggtcggca atcatcaaaa tcatcattaa aattttaaaa tcaaatattg aatgcgcgtt
    17581 tttgaaatag cgaaattcac ctgcgccaaa caaaacgcaa gtaattttat gtaatagtgg
    17641 aatgtagttt tatttcagaa tacaatacag gcatcggact ttatttcaca accgggagac
    17701 aaaatatgag catcaaagta gcgattaacg gtttcggccg catcggccgc ctcgcattgc
    17761 gtcaaattga aaaagcccac ggcatcgaag tcgccgccgt caacgacctg acccctgccg
    17821 aaatgctgct gcaccttttc aaatacgaca gcacacaagg ccgtttccaa ggtactgccg
    17881 aattaaaaga cgatgccatc gtggtaaacg gcagagaaat caaagttttc gccaatccga
    17941 atcccgaaga attgccttgg ggcgagttgg gtgtggatgt cgtcctcgaa tgtaccggtt
    18001 tctttaccaa caaaaccaaa gccgaagcac atatccgtgc cggcgcgcgc aaagtcgtga
    18061 tttccgcacc cggcggcaat gatgtgaaaa ccgtcgtata tggcgtaaac caagacatct
    18121 tggacggtag cgaaactgtc atttccgccg cttcctgtac caccaactgc cttgccccga
    18181 tggcggcagt cctgcaaaaa gagttcggcg tagtcgaagg cctgatgacc accatccacg
    18241 cctacaccgg cgaccaaaac acccttgacg cgccgcaccg caaaggcgat ttgcgccgcg
    18301 cccgcgccgc cgcgctcaac atcgtgccca acagcaccgg cgccgccaaa gccatcggtc
    18361 tggttatccc cgaattgaac ggcaaactcg acggctccgc ccaacgcgtc cctgttgcca
    18421 ccggctcgct gaccgaattg gtttccgtcc tcgaacgccc tgcaaccaaa gaagaaatca
    18481 acgccgcgat gaaagccgcc tccagcgaat cttacggcta caacgaagat caaatcgttt
    18541 cttccgacgt tgtcggcatc gaatacggct cgctctttga cgcaacccaa acccgcgtga
    18601 tgaccgtggg cggcaaacaa ctggtgaaaa ccgttgcctg gtacgacaac gaaatgtcct
    18661 acacctgcca actcgtccgt actttggaat acttcgcagg caaaatctaa ggcttgaaca
    18721 aacctgtgga taagtaaatg ccgtctgaac acattgcgtt cagacggcat tgtttatctg
    18781 taacttgata ttgcggctta tggcgaacaa acaacagccg tttgttcaac tgctatgcgg
    18841 caaaaccgga ttttgcccga tttaaagcgg ggttggggaa agattaataa ccgtcgcgcg
    18901 gttttcctcg atttgaaacc tgacttcgta atccgcaata ttcatcacat aaatccgttc
    18961 gggaatattc tgataggcgg ggcgcggatc ttgggcaatg ctttggctga taaggttttt
    19021 ggtgtttgca gataaatttt ccgcgccgat gttttcctgc caaacgactt ccaactctac
    19081 gggtttgccg ctgacgaaac cggatgcggc atcgggtttg gattcgataa aggggatata
    19141 aggcttgatg tccacaatcg gcgtgccgtc cagcaggtct gagccgctgc aatagaggcg
    19201 gacgggtttg ccggtttcga tgcgttcgag tttaaggaga gagagtccga gatggttggg
    19261 gcggtggggg ctgcgcgtgg cgaacacgcc cattttttgt ttgccgccga gccgtggcgg
    19321 gcgcaccatt tgcgcccaac cttcatccaa tacgccgtgg aagataaaac ttatccacac
    19381 ataatcgaaa tcttccaacc cgcgcacgct gtctgcggta aattcgggat tcagctcgat
    19441 gcaggcttct gcggcggaga ccaaaccggg ctggcgggcg atgccgaatt tctgtttgta
    19501 gggcgagcgg gcggtgccga tgggggtaat ggtataagtc ataagattgt ggataagttt
    19561 tttggaaaaa ccttatctta tcacagccgg ttttaccgct ttcgggtagt aatttgttat
    19621 aatacagggc atttaaatcg cgttgaaata aagtagcaca gaatccttgt gtccaagacg
    19681 ctcttaaaaa ggcattggag tattgataaa aaagaagcat aacggttcgt tataacgaag
    19741 agggaatccc gtatattaca cccaagtcag gttgttatct ttccttttgg atctcactta
    19801 caggaggtga gatctgaaat tttgggtaat aaaatcggat tacagcttct agcaaggagt
    19861 accttaggta tagctaaaat ggagggggtg gatatgacat atcatatcca cctagatgga
    19921 ttgtgctcct taaataaaga aggtaagagt tttgttattc gtaaaataga gaatgacgat
    19981 cttaagtgaa gaagtaatga gatgattgtt tccattgatg ttgatgcaca aaaaacgttt
    20041 acgccactgt gtcctgacga actgcccgtg aacgaggggc atttgattgt cgaggagtta
    20101 aatgcccaag ccgctttggc ggatttgcgt gtgatgacga aagatgcgca tcatatggcg
    20161 gcgaaatggc ttgtggataa ccctgttgat atgttgaagc cgacaggttt gtccgatgcg
    20221 gatttgactt gggtggctca tgcgatggtc ggtacgtgcg gctatgaatt gttggacggt
    20281 ctgccttccg ccaaggaata cgattattgc gtttggaaag gtgtcgatcc cgaattgcat
    20341 ccttacggcg cgtgttttca cgatattgag gaaaaactga gcactggatt gattgagtgg
    20401 ctgcgttgtc aaaatacgaa tatggtcatt gtcggcggct tggctacgga ttattgtgtt
    20461 aaaacaacgg ttttgcagtt acttaaaggc ggtcgttggc aggttatcgt gaatgaagcg
    20521 gcttgtcggg gtattgcgcc cgataccatt gaagcagcgt ggcaggaaat gcgttcttcg
    20581 ggtgcgataa ttttgaaaaa cgccgaaaaa attaaaaaat atattaataa taaataactt
    20641 ataattgttt cacgtgaaac accctgtcta catatgaaaa ttttacttgt ccgtttgtcc
    20701 agtatgggtg atttaattca cactttgccc gcaatcgaag atttggcgcg acaatgctct
    20761 gatgtagaac tgcattggct atgtgaagcc gggtttgcgg gtattgcacg gttgcatccg
    20821 tttgtaaaaa aggtccatgt aatgaaatgg cggcaatggc gcaaacatct ctttcaggct
    20881 gaaacttggc gggagatggg tcgtttgaaa caggctttgc tgcaggaagc atttgatttt
    20941 gtattggaca gtcagggtct gattaaaagc gcgtgtttcg ccaaaatggc aaaatcccct
    21001 atttatggtt tggataaaca cagtgcgcgt gagggggtgg ctgctttggc gtatgataaa
    21061 aagtatgtcg taccgaaagg tagaaatgcc gtttggcgca accgtgatct gtttgcccag
    21121 gtgtttgggt atgcaatgcc tgagacgcag gtatttggtt tgtccgttcc tgaagcaagc
    21181 cgtctgaaaa atttagcgca gccgtattat gcggctttgc acgcgaccag tcgggacagt
    21241 aagttatggc ctatggaaaa ttggcgggag ttgctgcaga aactgaatca aaaacaacaa
    21301 tgcaatgttt atctgccttg gggaaacgag gctgaaaaag tgcgtgcgga acagattgca
    21361 gacgggctgc cgtttaccat agtgtgcgct aaaatgaatt tattacaggc ggcgtatctg
    21421 ttgaaacacg cggttggaat agttggtgta gataccggtt tgctgcattt ggcaaatgcc
    21481 ttggaaaaac ctgtggtcgg catttatacc gataccgatc cgattaaaac aggcgttcaa
    21541 gtttcggcta ttgcaaaaaa tgtgggcaat atcgggcaga ttccgaccgc agatttggtt
    21601 tatcaaacgc tgatggattg tgtttcagca gataaaggct aaagggtcgt ctaaaactga
    21661 tatggcggca tgatattttc caatttatta gatacaggaa tgtatatccg ccagcctgag
    21721 taaatacttt aaaaatggta taatcggtaa aattatccga aagattgcta aataaattcg
    21781 tttttaagga aaacggattt gattttataa agaagaaggt ttggtttcgc tggatggtga
    21841 aagcagcgtt ctttttagga gaattcaatg aaagcactgg tcgcagtaaa gcgcgtagtg
    21901 gactacaacg tcaaagtccg tgtgaaagcc gatggttcgg atgtggacat cggcaatgtc
    21961 aaaatgtcga tgaacccgtt tgatgaaatc gctgtggaag aagccgtccg tttgaaagaa
    22021 gccgggaaag taagcgaaat tgtagcggtt tctttgggtg aaaagaaatg cgaagaaacc
    22081 ttgcgtaccg ctttggcaat gggtgccgac cgtgccattc atattgaaac cgatgtgaaa
    22141 ttagagccgc tggcagttgc caaactgctg aaagctgttg cggacaaaga aaatccgcaa
    22201 attttctttt tgggcaaaca agcgattgat gacgatgcca atcaagtggc gcaaatgctg
    22261 gcagctttgt tgaatgcggc gcaaggtaca tttgtttcaa aagtacaaat tgaaggtgat
    22321 gaagtacaga ttgtgcgcga aattgatggc ggcgaagaaa cgattgcgtt gaaactgcct
    22381 gcagtcatca gcgcagattt gcgtttgaac gagccgcgct ttgtcaaact ccccaatatt
    22441 atggcggcaa agaaaaaacc tctggaaaaa ctggattctg ccgatttggc taccgatatt
    22501 tcgcctcgtc tgaaaacggt gaaatttgcc gaacctaagg cgcgtcaggc aggcgtaaaa
    22561 gtagcaagcg ttgccgaatt ggttgaaaaa ttgaaaaacg aagccaaagt gatttgagga
    22621 ggctgaaatg agcgtattga ttattgccga acacgacaac aaacagttga atcctgccac
    22681 tttgcatgct gttgccgctg ccgccaaact gggcaaggtc gatttattgg ttgtcggaaa
    22741 cggcgcatcg tccgtcgtgg aattcgcgaa gcaagtagcg ggagtggaaa aagttttggt
    22801 tgcagatgct gctcattatg ctgaaggttt ggctgaagag ttggctccgc tggttgtcaa
    22861 attggcggcg gattaccgct atgttgcagc aacggcaacc acattcggta aaaacctttt
    22921 gccccgcgta gccgccttat tagacgtacc gcaaatttct gatttgaccg agattgtaga
    22981 taacacgact tttgtacgtc cgatttatgc aggtaatgca tttgaaaccg tgcaagccga
    23041 ttcagaaaaa ttggtgctga ccttccgtgt gacggctttt gacgcagtgg cggcgcaagg
    23101 tggtaatgct gaggtaatta atgttaaaac aacccctgcc caaaacctga gtcgttttgt
    23161 taaccgtcaa ctttcccatt ccgatcgtcc cgaactgact caggcaaaag tgattgtttc
    23221 cggtggtcgt gcgttgggta gtgtggaaaa attcagtgaa gtgctgacac cgttggcgga
    23281 tgttttaggt gcggcaatcg gtgcatcccg tgcggcggtt gatgccgagt atgcaccaaa
    23341 cgatgctcaa gtcggacaaa ccggtaaggt ggttgcgccg cagctctatt ttgcaatcgg
    23401 tatttcaggt gcgattcaac acgttgccgg tatgcaggac agtaaggtga ttgttgcaat
    23461 caacaaagat gccgatgcgc cgattttcaa tgtagccgat tacggattgg ttggcgattt
    23521 atttgaaatt gttccacaat taatagaatt attgaaaaat tgatgtttca cgtgaaactt
    23581 tcaggcgact ttgttattaa tgaggtcgtc taaaaacatt tcagcatgat tacgacttat
    23641 aaaaccatta cttccccgac gcaggctgag tttaaagata aaggcagccg ttttattgca
    23701 tttgcctatc cgattcggac attggctgat gtgaaaaaat acctcaatct gttaaaggaa
    23761 gagcattata aagcgcggca ttggtgctat gcctatcgtt taggtgtgaa cgggatgcag
    23821 tttcgcggca acgatgatgg agagccacca ggaagtgctg ggcggccaat tttgggacag
    23881 attgattcgg tgggtatcac tgatgttttg gtcgtggtcg tccgctattt cggtggtgct
    23941 ttattgggcg ttcccggcct gattcacgca tacaaagagg caacggctca ggcgttggcg
    24001 atcgcggaag tggttgaaaa gaatattgaa aaaaccgttt ggctgaaatg tgagtatccg
    24061 tttttgaatg aagcaatatg cattgccaaa caatatcagg cggatatatt ggagcaggat
    24121 ttgcagttgg attgccgatt gacggtaagt ttgtcgttgg caaattatga ggcctgtgtt
    24181 gcggcttgga agaatacacg gcaggttgaa gtaaatacgg aaaagccttt tgaatgaaag
    24241 gttgtatgaa atgctttgta atggtatttc aggcaacctc ttcgctgatt aattttcttg
    24301 tgtatatctg tctaaggtat cgacgctgga gcataaatat agccaagcca attgatggaa
    24361 ttcgcccggc gcgttccata atgcatcttc actcataatg ctatattcgc cttctgtttg
    24421 caaattcagt tctaccccgt cttcaatagt ctgatggcgc acctgatact cgtgggtata
    24481 gaaattgtcc atatcgcgaa ttaaagcgac ggcatatttc caacggcgga tgttttcttc
    24541 cagttgcagt agtaaatgac accattcgcg gtatttgctt tggatttcat caatacgttt
    24601 ttgcgtgttt gcttgttctt ttttaatttg tgaataagtt gaataggttg cagagtggaa
    24661 tgaagtggtc ttttgtttga ttctactaaa atttcttcag atgggacaaa agttcctttg
    24721 tatcgagttt tcgttaaaag tacatatttg tcaaggagat ggcatgaaac tgctggttat
    24781 cggtaatggc ggtcgcgaac acgcgctggc ttggaagttg gcgcaatcgc ccaaagtgga
    24841 aacagtattt gttgcgcccg gtaatgccgg tacggcgatt gaatccaagc tccaaaacat
    24901 tgccttgacc gcgtatcagg atttgattga attctgccgt aaagaaaata ttgtttttac
    24961 cgtcgtcggc cctgaagcgc ctttggcggc aggtattgtg gatgattttc gtgccgcagg
    25021 gctgaaaata tttggtccga cacaatatgc ggcgcagttg gaaagttcta aagatttcgc
    25081 caaagcattt atggtgaaat acaatattcc gaccgcgcaa taccaaacct ttgaaaatgc
    25141 cgatgccgca catgattacg tcaatcagaa aggtgcgcct atcgttatca aagccgatgg
    25201 tttggtggca ggtaaaggcg tgattgtggc aatgactttg gatgaagcgc atgctgcgat
    25261 tgacgatatg ttgttgggca ataaaatggg caatgccggc gaacgcgttg tgattgaaga
    25321 ttttctgcaa ggcgaagaag cgagctttat cgttatggtt gatggcaatc atgtattacc
    25381 tatggcgacc agtcgagacc acaaacgtct tttagacgga gataaaggcc cgaatacggg
    25441 cggtatgggt gcgtacagtc ccgcgcctgt ggtaacgcct gctgtgtacg agcgtgcgat
    25501 gaacgagatt attttgccga ccgtagcggg tatgaaagcg gaggggcacg agttcaccgg
    25561 ctttttgtac gcaggtttga tgatcgatca aagcggtgca ccctatacga ttgagtttaa
    25621 ctgccgtttc ggcgatcccg aaacccagcc gattatgagc cgtctaaaca gcgatttggc
    25681 ggatttggtt gaagcggcaa tagacggcag actggacagc gtcaaagcgg aatggaatcc
    25741 gcaaactgcc gtgggtgtgg tactggcggc gcaaaattac cccgaaacac ccaagaaagg
    25801 cgatgttatt tccggtttgg atgacgtcaa ccgaatcggc aaagtattcc atgccggcac
    25861 aacggtaaac gagaaaggcg acgtgttgac caacggcggg cgcatattgt gtgttgtagg
    25921 attgggcgac gatgtggcgc aagcgaaagc caaggcgtat ggcgcgttgg aaaaaatcag
    25981 cttcgacggg atgcaatacc gtaaagacat tgccgataaa gcgattaacc gttaattaga
    26041 aagaggatga tgccgtctga agccaagtat gactgatgcg cttcagacgg cattttatat
    26101 tatgttattt tcgagaatta tcgcagcatc agcgcaacgt aagttaagcg tttatttgaa
    26161 aaaaggcggt ttggttgctt atccgaccga atcttgttat ggcttggggt gtcttccgac
    26221 attagccaaa gcactcggta agttggcgca tttgaaaaaa aggccgcaac acaagggtat
    26281 gattgtcatc gggaatcaac ttgaacaatt gcagccttta cttcaaatgc cgtttgaaaa
    26341 cattcaaacc atgcttagaa acgaatggcc tgctcctaag actttcctat tgttggctaa
    26401 atctggcgta ttacctgcgt tgcgcgggaa gaggcgcagc aagcttgcgg tcagagtgcc
    26461 cgatcataca ggtgcaaggc gtttgtgtca ggcattgggt atgcctttgg tttccacttc
    26521 atgtaatcgg gcgggtaaac gcgcgtgtcg gacggagagg gaagtcagaa ggcagttcgg
    26581 acgcgatgtg tggattgtcg gtgggagaat cggcaggcag aaatcgccga gtcagattat
    26641 cgatggggaa acaggtaaac gtttgcgtta gatatggatg cttgagtgaa aacttttttg
    26701 catacacaaa gaaaaaattt tttggatggt atttctctac atgattgtta tcgcacggcg
    26761 ataaaggtag aaggacgcaa cgtctgcttt gattttgagg acggttttgt cgttctggac
    26821 aataatccga acaatcaagc gggcaagcat ctgaaaacag atttttcccg tgttgtttta
    26881 acccatgaaa atagaaacgc cgaagacgac tatttggtcg atatttttga agacatcgtc
    26941 tttttaggga aacgtttgtt tacagtcaga aaatttcttg atttttctga attgcttgaa
    27001 atgatcaatg cacaaggcta ttttcttgaa tttctgtatc tgtatgaatg tatcggtgtc
    27061 aaaacaagcg attatttttt agaggctgtg ttggttacgg gacggagccg ggaatgtaaa
    27121 aaatgcttta tcaaaattat gagggcaagc agttttgtgt atcaatggaa caatttacgt
    27181 ttgaatagtg agattgtata gcgggtttat atttcggatg gaagccggtt taaaaaaata
    27241 atgtggataa tttcagggaa tggcaaaatg tcgtctgaaa tgctgatatt tcagtattcc
    27301 agacggcatt tttataataa ggcttatgga taaatttgtg tataagttga aatcagagtt
    27361 ttttcccgca tacggtttca tattgttttt cagttaatgt cccggacagg atgctcatcg
    27421 ggcggaggga agaggctgtg tagttttgtg tggaaatttc cgtgtttttt gtatcaaata
    27481 actgtagcga acttaagcgg tatgttttgt tgttgcagtg gatttcccac tcggcaatgg
    27541 cagtcttgta tgcgggggtg ttggcaaaac gttcttgttt cagattggta acaacttttt
    27601 tatcttggaa aatcatcaga tttccgtttt ttctcacact gtctttatta atatatgtat
    27661 aaatattgcc gtttgagatt gtgccgatat tttcccatcg ggcagacttg tccggtgtgc
    27721 tgcaggcggt tatcaaaata gggagtaaaa cgaaagatag gaagattggg cgcattaggg
    27781 tttccttaat cattgaaatc cg
//
LOCUS       DAXFZR010000017        26930 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000017 DAXFZR010000000
VERSION     DAXFZR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26930
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.017"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            185..994
                     /locus_tag="ACFJAS_000638"
     CDS             185..994
                     /locus_tag="ACFJAS_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC3981951.1"
                     /translation="MHTTHFLMMTATIATALPTFAEPPPPINYSQMRFNDLAQQCSKD
                     VHPNTLQAVARVESRFNPYAIGVVRGSLKRQPRNFAEAVATAKSLHAQGKNFSMGLMQ
                     VNRYNLAAYGLNYETVFEPCKNINAGAQILKDCFDRAGGNGQEALQKAFSCYYSGNFR
                     FGFKSDFKGQPPYVTKIILAAMDNSPYQTIGFSGSLKTSGRRSGQSSLLRTSYVPQPS
                     NSRQPETYSEPPAEQQVAQPNSVTEKPVSVSSAETQTVAKATAAWDVFADF"
     gene            1056..1385
                     /locus_tag="ACFJAS_000639"
     CDS             1056..1385
                     /locus_tag="ACFJAS_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFC3981952.1"
                     /translation="MKTNTFMRISQSKTTKFMAMVALGLVAQSALAAGGFANEAKTMV
                     ENIRNGIYIVVGVLATVALLWQFAQGFMGRKTWGDILESSMWIVGAGAAIVLGTWLFT
                     KGGSMSF"
     gene            1396..1824
                     /locus_tag="ACFJAS_000640"
     CDS             1396..1824
                     /locus_tag="ACFJAS_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFC3981953.1"
                     /translation="MSNYEDINTEYATYNALGRQAMIFGVPVVGLAASGFVLVMATML
                     AMPFLNGRALLILALLIPIFIGLRTVCETDDQALRIYAYEIKWFFRKRNAKIFNNTTT
                     IIPTKYGRQNSDFQRFFQQDVQAAARTFRLTAANLPTRYQ"
     gene            1733..4138
                     /locus_tag="ACFJAS_000641"
     CDS             1733..4138
                     /locus_tag="ACFJAS_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC3981954.1"
                     /translation="MIFSDFFSKTFRQQPAHSDLLPPICQHVTNNIVWYETGHVGFVI
                     RMNGLPFDGVEDNYLYTQFVGLKNMLAGMGKTLGNRLAVWTTLQRKKINFNREYSFKT
                     PFCQQFADKYLQRFHVENYFENVFHITVLIKSSDIDSGIKEAEELMASMMSSLTPYEP
                     TLLAAYQNQNGILFSDVYRFFGSLINGQDEPVPLSFMDTYQTLGSATLHFGSDIGEIR
                     PMHGQRKFVTCYDLKDFGISKPKILTDILTLPCEFTLTQSLIYINSHTMQANIRKQLN
                     NLKSVGDQAIEQQEELYSGMGKLTAGELMFGDYHAALVVYGDTPQEAAGNGSKAYAAF
                     MNSGGYRFTKAGASALSTYYSQVPCSRERPRSFPKTTTNLATTFGIHNYSCGKSKGNP
                     IGDGSAVMPLQTKSRTLYDFNFHFSNPQEDNVGEKIAGHTLILGATGTGKTTLQTSLM
                     TFVERFDPYIFALDLDRGMEIFIRVLGGQYFALEAGVPTGLNPFQLPDTPSNREFLYT
                     LVGLCGTNHNGTLTAEEEKQIQVAVDTLMGLDHELRNFSHLLQNIPMVNQENSLRERL
                     GRWCRSENGRFAWCLDNDANLFDAEQFYRVGFDLTDILKDDYPPTAPVLAYMFHLRGI
                     MMKGVAEKNGLLASIIEEFWWAARFPTLQELMLKILKTDRKLGGWLILVSQSPEDAIS
                     CPIFPAIVQQTPTKIFLPNPDAEYKNSYERCGITEKEYRELVELSVDSRTFLVKQSTQ
                     SAFAKLDLQGFSNEMAVLSGSSDNVELLHRVIADCGSEAPNVWYEPFIQAVHKRRAIK
                     KRV"
     gene            4220..4867
                     /locus_tag="ACFJAS_000642"
     CDS             4220..4867
                     /locus_tag="ACFJAS_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFC3981955.1"
                     /translation="MKTKLTKMAAVMLSGCLMCSSVSAGGIPVFDDASVAQAIKQGIQ
                     LKQQIENQISQIQEMRNQLAAIKGSRGMGNLARRAIDYDLNIPSEWADLHKLTSTQQQ
                     ARLKGSQYDAAQYDNLLNQNLTMLSKFTADNQQRLNTIDRLINQINAATDIKASSDLR
                     NRLSAEQLKFQNQQTMLDQMARMYDLQEKVQARQYSNYEACLARHMKSRDYSACK"
     gene            4896..5894
                     /locus_tag="ACFJAS_000643"
     CDS             4896..5894
                     /locus_tag="ACFJAS_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFC3981956.1"
                     /translation="MADVICRKYKAIGYIMFDNIFESLLKGMGSDLFAKTANIITAIS
                     PLFAAGFGIYIVIVALNAYNRGFDDNLIDLAKRAMGWLIIIACAFNAAQYLKIANMLY
                     ELPEYMSNLINPGAYNAAVLDNNIDKLKDSMGKVWELKSEIPPLAIPSHLAFMFMWVF
                     IFILVALFLTAIAAFYLVAKISLAMVILIGPIFIGAMLFPATRQWGMNWIGQIMNYAV
                     TVTFYVLLGTIQITFFEAHMLSMIETDLIKVADKGSISLVVFIPIAFMFILSTIVFFV
                     VALSIPSIASALTGGATIGGYSGIGRTISSASNSLTKRSGSFKSNNPNNQIGYNKS"
     gene            5909..6490
                     /locus_tag="ACFJAS_000644"
     CDS             5909..6490
                     /locus_tag="ACFJAS_000644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981957.1"
                     /translation="MASKIIWQNSYCYLTLFEGQMADLHQRSETVITGGGHHIGSYTN
                     TLTQIFIIDKQGNQYTFGTMNWDIPMRVGNDLKVITICRNNWANGEIVLAQNISLNQI
                     SWSSERLKSAIKRVYFPFLKWGLVTLFVIPFILYFVLFVFIQPEPNSFIDTIFSLLMN
                     VCQLGGFGSIIYGYHFCWRKAYSQANKSLAINL"
     gene            6555..6689
                     /locus_tag="ACFJAS_000645"
     CDS             6555..6689
                     /locus_tag="ACFJAS_000645"
                     /inference="COORDINATES: protein motif:HMM:NF019749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC3981958.1"
                     /translation="MKKLLITLTATALLAACSHNPPKPYGKPFPINPQPSITTEKANP
                     "
     gene            6686..7405
                     /locus_tag="ACFJAS_000646"
     CDS             6686..7405
                     /locus_tag="ACFJAS_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFC3981959.1"
                     /translation="MKTNSNKTPKAPNPQQQGLKFIQEAKEFHKSEIDRVRKNSRIAW
                     RISGVCLVITGLAVGAVAGLTPLKTTQPFLVRVDNNTGVTDVVTTLKTAEKTYGEVVD
                     KYWLAQYVRYRESYDWQTIQATYDAAMLLSDPTIQAEFAKLYNNPTAAPHKILRDQYK
                     VIVKVNAISFLGNDVAQIRFEKQTIPTNSVNQPLPPQRFIATVGYAYKNEPQEEKDRL
                     INPLGFQMTSYRVDPENTPNQ"
     gene            7415..8230
                     /gene="virB9"
                     /locus_tag="ACFJAS_000647"
     CDS             7415..8230
                     /gene="virB9"
                     /locus_tag="ACFJAS_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HFC3981960.1"
                     /translation="MKNKLIYFTIAALMLPFSVQAASIPKGSPKDRRIQTAVYTPDDV
                     FVVRTKVGTATIIQLESDERIIGESSAMGIGDAEAWKMTARGQNIFFKPTAERPDTNL
                     LITTNKRTYAFDLKSTNGTPPPTYILRFHYPDTENDKRRIETQKRIRAAEVFGKVQAA
                     PPLGYNNDYWGRGNKNLAPTSIYDNGRFTYFRFNNGRDLPSIYKVLDDSSEALLNTHM
                     DGDTVVIHETAQHFILRLGNQVLGIENRGFSSNGQFNQTGTDDNSSIRLIKGE"
     gene            8232..9566
                     /gene="virB10"
                     /locus_tag="ACFJAS_000648"
     CDS             8232..9566
                     /gene="virB10"
                     /locus_tag="ACFJAS_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HFC3981961.1"
                     /translation="MLKNIFNRKKQESNLPEPDYEQPENAENSIETDEPKPLSEQIND
                     VNVEAGLPLDSSGGKNNMTKVAVLAVALVGVGIAVAGMIAFSSGGDDDEAVAAQQQEL
                     ERVQKTQAKDFSTEKLDFGQEEIAASEPVAASQAGTAEAQTVPPIETTEAPPPTETAP
                     PPATQPETAQTAEPKETPRDRKLRGDVLIGEKSSDMDSTAQASDTEQGAFQATSFSEK
                     PSATQENSQSQSSFASRLNPSVTPSMKAQRRADSTYLLAKGTNIPCTLDTQIITTHAG
                     ITRCLVTKDVYSANGKVLLLERGTKIIGEQNSAMLHGQARVFVLWNEAETPKGVKVSL
                     ASGGAGQLGASGHGARVKYHFWQRFGGAVLISLIGDLGDYYSNRSQSNGQNISFENSS
                     ESAQELATEALKNSINIPPTGFINHGTLINVMVARDVDFGGVYEVVKPYEIF"
     gene            9617..10714
                     /locus_tag="ACFJAS_000649"
     CDS             9617..10714
                     /locus_tag="ACFJAS_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFC3981962.1"
                     /translation="MQPTQTIQADTSARNALDRLGISDYLNRDGVTEVIINRPNEIFL
                     ESSSGWQCFQDERLNLKALEHLANTLCVFNSKHISTANPIHSVTLPDGERGHIMIPPS
                     CEDKTIVYAFRKPSKTRFSVDDYINTGRLSGFRDSAQFGVSGSLKTEIDGRHFRDMAD
                     KMNLPPDVPLADWQYEMLTHKANRDLNAFFRLAIEHKLNICMVGGTGSGKTTFTKAIS
                     DLIDPTTRIITIEDTHELDLPKHPNHVHLFYKEHITAKQIIAACMRLKPDRIFLTELR
                     GDEAWDYLSALNTGHAGGLTSVHANDAQSVFYRIAQLAKESETGRTMDYEFILNTVRS
                     TIDIVCFFDRTRMTELYFDPVAKFRALSGRS"
     gene            complement(10943..11260)
                     /locus_tag="ACFJAS_000650"
     CDS             complement(10943..11260)
                     /locus_tag="ACFJAS_000650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981963.1"
                     /translation="MVKKTGLDNFVLEYKKLSEEVALLREQQKQQSRFFENLALWLVK
                     VGIHNQSTEQIIELVEIVDNFYLNGQTDFGCDDFGFWQKLDKLIKDNSVDDEPVLSES
                     PSE"
     gene            11284..11658
                     /locus_tag="ACFJAS_000651"
     CDS             11284..11658
                     /locus_tag="ACFJAS_000651"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC3981964.1"
                     /translation="MMFYNRIQIMGNLGKDPEIRYFADGTPSAKLSVAYTEKWRDKQT
                     GEPRERTEWFTVLLTKNQAETAAKYLHKGDTVQVAGKMQSRHYTDKDGAERTVYEIHT
                     NELLIIKTRKSNDLEQADAPEN"
     gene            11679..14066
                     /locus_tag="ACFJAS_000652"
     CDS             11679..14066
                     /locus_tag="ACFJAS_000652"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFC3981965.1"
                     /translation="MSKTLIIAEKPDMAKSIAAALGIPKAKGCYENDRFIVSNCIGHL
                     IELDVPKWRDKNASLPVIPQTFSLKIKETTAQHYQFLRGLLHRADVSMLVNACDADRA
                     GEHIFRLVYQHSGCLKPVKRMWIQSTTQQGLRQAFENLKNGADYDHLAAAEQCRAEAD
                     WLLGMNGSRALGNQFGRVMTPTLAMVVDAYLANQNFKAENYWEISVQFSIKSGSYNAK
                     LLDENDKIARFADDKTAQAACEQIRNQNTFAVNDVAETEFSHAPYLFDANELQKIANK
                     KLGFAADKTLSIMQSLYEKHKVLTYPRSDFNALPDDYLPTLTATISGLGALAEYGNIV
                     NDIQAHDLIKHNPRVFDDKRIGSHYAIVPTGFIATADGEHALSDCKPDVLKNTLSADE
                     YAVFHLVALRTLAALYPAAEYGVTTRTTVSGSLKFRTTGRVLLKQGWLSVYAGIDDNN
                     GSSENVPSEKLPPLSSDEMAQPDTISSKKLTTKAPPLMTESRLIQAMKTAGRDLDDKH
                     LADLMKEIKGIGTSATRAPTIKKLKEPTAQGDTAYLSLDKNKLIPSQRALDAIAHIRR
                     FFPETADPILTAEWEDKLHQIEHGQFPPNQFRTEIHQFVRDFVATVLPHRPSHHSTEI
                     LCACPNCDSGSLKEQKFSWNCECGFKISKEQRGRAMSASELRQLISTGQTDVLQNFVS
                     KEKGTKYAAMLKLERDDSGSLKIAVAFPERKTEVCPCCSGSLNDKGKLYECACGFKLW
                     KTQFGKTLTEKQLKSLIDNGETGLIKGFHKKDGGTFDAVLCVDKANKKIGFPPRK"
     gene            14125..14310
                     /locus_tag="ACFJAS_000653"
     CDS             14125..14310
                     /locus_tag="ACFJAS_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HFC3981966.1"
                     /translation="MDKDNLSDMTRNIIGSWRLENLEISTETIADMEALAHGNLTPEM
                     GIERVRQRILQDNGHEK"
     gene            14300..16312
                     /locus_tag="ACFJAS_000654"
     CDS             14300..16312
                     /locus_tag="ACFJAS_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFC3981967.1"
                     /translation="MKNKILAPILFILFTLPLAGAGGGFVGSMLFVRWQKLNSKPSVL
                     LLYEYAQHMDKMPAKLLMPFKVSVTISLLISILPALIILAAVFAKPKRELHGSSRFAN
                     AVEVQQAGLLKPKYDPKTDKPDLLIGKYKNKYLRWAGNEFLYLAAPTRSGKGVGVVIP
                     NCLHYRDSMVVYDPKLENFIITGGFRQQKLGQQVFLFNPGGRMPEHERNPMLPLVSHR
                     WNPMTYIRRDARYSYKDLSNMAAILLPKPAKDNGSGTFFVESARKLFVGLGLYMVETE
                     RERDLTDYTQRTTLANLFRLTAPTDGKPLNEWLLAEIAERDNDPEKTPLSSQCKTLLL
                     GFANGSPKTGADILASLTAPLGIFLDPVCEAATSGDDFRLDELRKKRMTIYIGVVPTE
                     TETFSRLTNLLFSQIIDVNVQQGLPQDNKDLLKYQCLLLMDEFTALGVIPAVQHGVSY
                     IAGYELRLLIIIQAPSQVEAIYGRENMETFFTNFTCRIFYTPRVQKDAEEYSKSIGYE
                     TFKARSTSRSQGKGGSRSTSISDQQRAVMNPDEIKMMPNSDCIINLGSSRAIYAQKIV
                     YWQDEVFKVRANLPRPEVPNLEIKTHRSRPKSTAAQTTKTEPVPDEKLPETDFKTCSN
                     ADDLFMAMVQSLVRPDSPPEFIKEIVEAMAQQWGKDSEPILAKMLT"
     gene            16401..20111
                     /locus_tag="ACFJAS_000655"
     CDS             16401..20111
                     /locus_tag="ACFJAS_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFC3981968.1"
                     /translation="MSDYNEIRNALNHLDANDRDFWVKMGAAVKDELGEDGFELWDEW
                     SRHSENYKSNDAKAVWKSLKQGHIHIGTLFYHARENGYHPNKPYTPPSAEELAKRAAE
                     TQEKQRAEAERIAQAHAKAEKAAYGIWKNASPADPNHAYAQTKGLGLNTDVMSGIRQN
                     EYQGKKQLIIPLYSKNKLVNVQTIDEDGHKKFLNGGQKQGAYTVIGDLKQSQNGIFLA
                     EGFATASSVYQATKKPVVVAFDAGNLKAVSEKLINVLPENVPVYFAADNDPSHTGKHK
                     AQAAAAIWGERAKIILPEFTPKQIHEFKEDYGTEKIPTDFNDLHKLAGIEAVRETFDI
                     EPEPYIIETETDIEFNRQPESNPQEIRTAWNDFPPVISNGKLGDLKNEPEYEAAKAGD
                     VMQSALLVDKLLKDETIQEIKNLIGDSKPIIVPIQSEEASGKNRIPQAMAFALSEKLG
                     LQVDTDIVQSNTAKRTNAKIDHRFVSPPEFTGTVKAGQPYLIVDDTLTVGGTIAALKG
                     YIENNGGKVIGSTVMTAHEQKADIVIKPNMLQSLQQKQGLNEYWRNEFGYSVDKLTQQ
                     EAGHLRKPTLEQIQERIEEAKIAKQASNISENKELHHERTTETQFAETRNSEVSNVFD
                     RAGSERIESIRQGDRKISQSVFQQEQTTEQIVNQPAQSATSEKDVADFSFKETKMDNL
                     QEHGKPDVPREELLKNNYWRVQNGYDPLSENDQKPEQTTTLGQTTYSADNPYAQWAEH
                     DDEVRHNADSWEMRTPLTNERLAHFNQQAEQYGFTTYIENRHAELGVFQADKWTNETY
                     HVEIDGKFKNHGSMAAYEDARDFTMSFHENDEIVHYVYTNHLDDALQQANDFLQNPAE
                     WIKEQDLKMQAEAMQPENAPPEYDYVPEYSSDDNEEPQPEMFNGIEYGGFQREIKQPE
                     KQPEKQPESQPDVEEPELPRSEALEHRPILDLTYERPPKHLQSRYIVAKNGQYLSADN
                     HTTVLFEDKGNALKTAKSDQQTIQDMLEVAKAKGWNSIKISGSPEFKSQMYVAAESQG
                     IKTTGYKPTPADLAMVEKLREERSLNGIQSTHKPELDPKQPENAPAHQSDAATLSAAD
                     KLKTQSEMPDERQINSSPIADTEAAPKVDSLGNKTIPSEVLRSTHEMTAAALSPEVET
                     SKSTYDQKAKQLSEPNQARLKAYELNTMDALRNVQGDARNHALRNFYEHTAKVMNGTE
                     LNLPQPMQNRAPTHHQAHEPQQSNSQDFEMER"
     gene            20178..20741
                     /locus_tag="ACFJAS_000656"
     CDS             20178..20741
                     /locus_tag="ACFJAS_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFC3981969.1"
                     /translation="MMKQQEIRVFGLSHDVINQLQLMALERYGKASASLMLRKLAEKQ
                     IRQPENIPTENYQENKQNQRLTLRLPPQQQHYLNQKAQLQYSSLNDVVRDIIAEYITQ
                     NPVLSNDEVQALYQSNYQLLRLGRNINQIARQLNSITPNSLTSQQLTELSAFLKQHAE
                     TVGKVLRKQNKPFKYRPIENIQNETDR"
     gene            20725..21768
                     /locus_tag="ACFJAS_000657"
     CDS             20725..21768
                     /locus_tag="ACFJAS_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traS protein"
                     /protein_id="HFC3981970.1"
                     /translation="MKQIDRRIDNWFLGYTRSVHTKSDSLNFGSRHSSKPLKTGTGLT
                     NLRVAAQKKPEVMVKIPKRHSKNSKGMKGIRNHADYVSRNGEIALENQDGEIFHGKRA
                     VKEFLNDWQSHCGIADETRHKEALNIVLSMPKETPPDALLQAARDFAAEQFQGHQYFL
                     GLHHNSHDPDEPEHPHVHLCVLMRDERGQRLNPRKNDLFEWRVRFAEKLRENGVDCAA
                     TKRVHRGKTQKLENSTIRAMRERGQSSKAELQQKLAISQAILSGKRPEHPFLNQADKT
                     RQKIVAEYKMIARELYMNGYKTEAKAMSKFAQEVSEKGFTTQAQQEFDKVQIQIRQPE
                     KSPDRSHDNEMER"
     gene            21863..22315
                     /locus_tag="ACFJAS_000658"
     CDS             21863..22315
                     /locus_tag="ACFJAS_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981971.1"
                     /translation="MKRITLSVLVLMLAACGGKNTDDYVGVWQRDDAKFLQFVEIKKD
                     GGNYLFSQGEQNYTFSEKNGELVLNTVFGEIPFKLSDDRKTLSANLYSSGSNRFFKVE
                     DESCKQLIAELQTAQEKLPLNAWSNEFKQASANIKAISEKYISQCSKK"
     gene            22465..22881
                     /locus_tag="ACFJAS_000659"
     CDS             22465..22881
                     /locus_tag="ACFJAS_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981972.1"
                     /translation="MVALNIRSPQGTGDWHSAATFASALPERVFVYGDNQPYNTNQLL
                     GAKGVIDGTKRLNSMGIFPENSPVFIADHPRACFDLLYTVVLQKGAINSVILDDWFPA
                     PEDKRAVYALIQAAEPYLNHQQKERLATWKQKNPIN"
     gene            23513..23911
                     /locus_tag="ACFJAS_000660"
     CDS             23513..23911
                     /locus_tag="ACFJAS_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981973.1"
                     /translation="MNDIETFLNQRNHAAKSDKVSVLEPYKDEIFQLKNMGYAEKIIV
                     DFLSEMKGVNVSRQAVNRFIRSRSAQMHNTAQNKPIMATDSVTMAQTAQAAETAKNQA
                     ENTPARRGLRKPTPTKKFNWEEKPDPETLK"
     gene            complement(24006..24284)
                     /locus_tag="ACFJAS_000661"
     CDS             complement(24006..24284)
                     /locus_tag="ACFJAS_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFC3981974.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRVQGGL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(24284..24478)
                     /locus_tag="ACFJAS_000662"
     CDS             complement(24284..24478)
                     /locus_tag="ACFJAS_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 domain-containing protein"
                     /protein_id="HFC3981975.1"
                     /translation="MQTTANTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELP
                     ESGEHTQYKLSHIVQDPKAA"
     gene            complement(24849..25121)
                     /locus_tag="ACFJAS_000663"
     CDS             complement(24849..25121)
                     /locus_tag="ACFJAS_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HFC3981976.1"
                     /translation="MQINWSTEANEDLNEITDFIADDSPVAAVNLSEHLFETAEKIGQ
                     MPYMGRKGRVAGTREFVAHPNYILVYQVKLDEIRILRVLHGSRKYP"
     gene            complement(25111..25308)
                     /locus_tag="ACFJAS_000664"
     CDS             complement(25111..25308)
                     /locus_tag="ACFJAS_000664"
                     /inference="COORDINATES: protein motif:HMM:NF045321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981977.1"
                     /translation="MNTALKLHDDPLVYEFSNKTDAADYDEWFRAKVNAALNSDKPAI
                     PHDEVVAKMRAKMAERLRNAN"
     gene            complement(25911..26693)
                     /locus_tag="ACFJAS_000665"
     CDS             complement(25911..26693)
                     /locus_tag="ACFJAS_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3981978.1"
                     /translation="MMLTADIRQHLTPNFTAIYVEFFALCGQNSEAALVLANLFGWSE
                     WLDNHRPKKQGWITKTAEDFANELKLTRHKYEKARECLLEMGLVEYKRAGVFGKMAYR
                     LCREKLLELIYTKVRGMSKPEFQSAFHTDKDGYHIPRFIPLHEWNEFLKMRESKSKSK
                     QSLVAEQKKQLIRQLTDLRKQKFDLKNVMQKSLISGWAGFYRPDKSAPSHAPAPNSSA
                     VEYEVFMKQQEAERKRQQSKKPRDLNTVGRLNLLKNLGLSKK"
     gene            complement(26706..>26930)
                     /locus_tag="ACFJAS_000666"
     CDS             complement(26706..>26930)
                     /locus_tag="ACFJAS_000666"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC3981979.1"
                     /translation="KWTTRIFKMRNAVKLAAEFAGSQAALARVLGIQRSTVNSWIQGR
                     NKIPPETAIKIEHLTHSQITRKDLRPDLFD"
BASE COUNT         8237 a         5951 c         5765 g         6977 t
ORIGIN      
        1 aagatacaat ttaataaact gatttatttg taaaatttta aagtcgatgc ctgtacacac
       61 cgccacacac agccataaat aaatggcatt ctgacacagt ttggacacgg aggaaacata
      121 cttaaaacag ccatccatgc ggctatttta agtgtgtttt tttactttga taaaaggaaa
      181 atctatgcac acaacacatt ttttgatgat gacggcaacc attgcaaccg cgctgccaac
      241 ttttgctgag cctccaccac caatcaatta ttcacaaatg cgatttaacg atttggcaca
      301 acaatgcagt aaagatgtcc accccaacac attgcaagca gtcgcccgtg tagaaagtcg
      361 gtttaatccc tacgcaatcg gtgtagttcg gggcagcctg aaacgccaac cgcgtaattt
      421 tgccgaagcg gtcgccaccg ccaaatcgct tcacgcacaa ggcaaaaatt tcagcatggg
      481 tttaatgcaa gtgaaccgct acaacttggc tgcttacggt ttgaattatg aaaccgtgtt
      541 tgagccgtgt aaaaacatca acgcaggcgc acaaattttg aaagattgtt ttgaccgtgc
      601 aggtggtaac ggtcaagaag ccttgcaaaa ggcattctct tgttattatt caggaaattt
      661 cagattcggt tttaaaagtg attttaaagg gcagccaccc tacgttacta aaattatttt
      721 agcggctatg gataattcgc cgtatcaaac aatcggattt tcaggcagcc tgaaaaccag
      781 tggtcgccgc tcggggcaat catctttact acgaaccagc tatgtaccac aacctagcaa
      841 tagtaggcag cctgaaactt attccgaacc accggcagaa cagcaagtgg ctcaacccaa
      901 ttcggttact gaaaaaccag tttccgtctc gtcagcagaa acgcaaacgg tcgcaaaggc
      961 taccgccgct tgggacgtgt tcgctgattt ctaacggcat caatccacac cgccaaatgg
     1021 cggttttttt tatttttaat ttaaggaatt tttctatgaa aacgaatact tttatgcgta
     1081 tttcacaaag caaaaccact aaattcatgg caatggttgc attgggtttg gtcgcacaat
     1141 ccgcactggc ggcaggtggc tttgcaaatg aagctaaaac aatggtggag aatatccgca
     1201 atggcatcta cattgtggtg ggtgttttgg ctacggtagc cttgttatgg caatttgcac
     1261 aaggttttat gggacgtaaa acatggggcg acattttgga atcatctatg tggattgtcg
     1321 gcgcaggtgc tgcaatcgtc ttgggtacat ggctgtttac caaaggcggc tcaatgagct
     1381 tctaggattg aaatcatgag caattacgaa gacattaaca cagagtatgc aacttacaac
     1441 gcattgggca gacaagccat gatttttggt gtgcctgttg tcggtttagc agcaagtggt
     1501 tttgttttgg taatggcaac gatgttggca atgcctttcc tgaacggtcg ggcattgctg
     1561 atattagcct tactgatacc gatttttatt ggtttgcgaa cggtgtgtga aactgacgac
     1621 caagcattac gcatttatgc ttatgaaata aaatggtttt tcagaaaaag aaacgccaaa
     1681 atattcaaca ataccaccac cattattcca accaaatatg ggagacaaaa cagtgatttt
     1741 cagcgatttt ttcagcaaga cgttcaggca gcagcccgca cattccgact tactgccgcc
     1801 aatttgccaa cacgttacca ataatattgt gtggtatgaa acaggtcatg ttggctttgt
     1861 gattagaatg aacggtttgc cttttgacgg tgtggaagat aactatttgt acacacaatt
     1921 tgttggcttg aaaaatatgc tggcaggtat gggtaaaacg ctgggtaacc gtttagccgt
     1981 atggacaaca ttacagcgca aaaaaattaa tttcaaccgt gaatactcat ttaaaacgcc
     2041 attctgccag caatttgccg ataaatattt acagcgtttt catgttgaaa attattttga
     2101 aaatgtgttc catatcacag tgttaatcaa aagcagcgat attgatagcg gcatcaaaga
     2161 agccgaggag ttaatggcaa gcatgatgtc atcattgaca ccttacgagc caacgctact
     2221 tgctgcttat caaaatcaaa atggcatact tttttctgat gtgtatcgct tttttggcag
     2281 cctgattaac ggtcaagacg agccagtacc gttatcgttt atggacacgt atcaaacact
     2341 tggcagcgcg actttgcatt ttggtagtga tattggcgaa attcgcccga tgcacggtca
     2401 aagaaaattt gtaacttgct atgatttgaa agattttggt attagcaaac ccaaaatttt
     2461 gactgacatt ctcacgctgc cctgtgaatt tacgctcaca caaagcctga tttatatcaa
     2521 ttcgcacacc atgcaagcca atatccgcaa acaactgaat aacctgaaat cagttggaga
     2581 ccaagcgatt gagcagcaag aagaattgta cagtggtatg ggcaaattaa ccgcagggga
     2641 attgatgttt ggcgactatc acgctgcttt ggttgtgtat ggcgatacgc ctcaagaagc
     2701 cgccggtaat ggttcaaaag cgtatgctgc attcatgaac tcaggtggtt atcggtttac
     2761 caaagccggt gcatcggcgt tatccactta ttacagccaa gtaccgtgca gccgcgaacg
     2821 tccgcgctca ttccccaaaa ccacaaccaa tttagccacc acatttggca ttcacaatta
     2881 ttcatgcggc aagagcaagg gcaatccgat tggggacggc tcagcagtca tgccattgca
     2941 aaccaaatca cggactttgt acgatttcaa ttttcacttt tccaatccgc aagaagacaa
     3001 tgtaggcgaa aaaatcgcag ggcatacact gattttaggt gcaacaggta caggtaaaac
     3061 cacactacaa acatcactca tgacgtttgt agagcggttt gacccctaca tttttgcatt
     3121 ggatttggac aggggtatgg aaatttttat ccgtgtactg ggtgggcaat atttcgcgct
     3181 ggaagcaggt gtgccgactg gtttaaatcc atttcaactg cctgatacgc cgtctaatcg
     3241 ggaatttttg tacacattag tcggtctttg tggcacaaat cacaacggta cattaaccgc
     3301 cgaagaagaa aagcaaattc aagttgctgt cgatacatta atgggactag accacgaatt
     3361 acgcaatttc agccacttgt tgcaaaacat accaatggtt aatcaggaaa atagcttgcg
     3421 tgaacgcttg gggcgttggt gcagaagtga aaatggacgg tttgcatggt gcttggataa
     3481 tgatgccaat ctgtttgatg ccgaacagtt ttatcgtgtt ggctttgatt taaccgatat
     3541 tctgaaagat gactatccgc cgacggctcc tgttttggcg tatatgttcc atttacgcgg
     3601 cattatgatg aaaggcgtgg cggaaaaaaa tgggctactc gccagtatca ttgaagaatt
     3661 ttggtgggcg gctcgtttcc caaccttgca ggaattgatg ttaaaaatct tgaaaaccga
     3721 ccgcaaattg ggtggttggc tgattttggt ctcgcaatcg cccgaagatg ccatttcatg
     3781 tccgattttc ccggcgatcg tgcaacaaac acccaccaag atttttctac ccaaccctga
     3841 tgctgaatac aaaaacagct atgaacgctg cggtatcact gaaaaggaat accgtgaatt
     3901 ggtagaatta tcggtggata gccgcacgtt tttggttaag caatccacgc aatcggcatt
     3961 tgctaaattg gatttacagg gcttttctaa tgaaatggct gtgttatcgg gttcgtccga
     4021 taacgtggaa ttgttgcatc gcgtgattgc agattgtggc agcgaagccc cgaatgtttg
     4081 gtacgagcca tttattcagg ctgtccataa acgtagagct ataaaaaaac gtgtttaaaa
     4141 attgctgttg tcgtgatgat aatagctgta aaccaaccgc ttaatgcggt tttttctttt
     4201 tgagaaggat ttttcaacca tgaaaacaaa attaaccaaa atggcggcgg ttatgctttc
     4261 aggctgccta atgtgtagca gcgtatctgc cggcggcata cctgtttttg acgatgcatc
     4321 ggttgctcaa gctattaaac agggtattca attaaaacaa caaattgaaa accaaatttc
     4381 tcaaattcaa gaaatgagaa accaattggc tgcaattaaa ggctcgcgtg gtatgggtaa
     4441 tttggcacgt cgagcgattg actacgattt aaacataccg agtgaatggg ccgatttgca
     4501 caaattaacc agtacacaac aacaggcacg tttgaaaggc agtcaatacg atgcagccca
     4561 gtatgacaat ttgctgaacc aaaatttaac tatgctgtct aaatttacgg cagataatca
     4621 gcagcgtttg aacacgattg accgcttgat taaccaaatc aacgccgcaa ccgacatcaa
     4681 agcatctagt gatttgcgta accgtttatc ggcagaacaa ctgaaatttc agaatcagca
     4741 aacgatgtta gaccaaatgg cgcgtatgta tgatttgcaa gaaaaagttc aagctcggca
     4801 atattcaaac tatgaagcgt gtttagccag gcacatgaaa tcacgtgatt attcggcctg
     4861 taaataatca tttttcttga tacatattcc gacaaatggc tgatgtcatt tgtcggaagt
     4921 ataaagcaat tggatatatc atgtttgata atatatttga aagcctgtta aaaggcatgg
     4981 gcagtgattt atttgccaaa actgccaaca ttattactgc catttctccg ttatttgcag
     5041 caggttttgg tatatatatc gtaattgttg ccttaaatgc ttataatcgt ggctttgatg
     5101 acaatcttat agatttagcc aaacgcgcaa tgggctggct tatcatcatt gcttgtgcgt
     5161 tcaatgccgc acaatacttg aaaattgcca atatgttgta tgaattgcct gaatatatga
     5221 gcaatttaat caatccgggt gcatataatg cggccgttct agacaacaac atagacaaac
     5281 ttaaagatag tatggggaaa gtttgggaat taaaaagcga aattccaccc ctagctattc
     5341 cttctcactt ggcatttatg ttcatgtggg tattcatttt tatattggtc gctttgtttt
     5401 taacagcaat agccgctttt tatttggtgg ctaaaatatc gttggcaatg gttattttaa
     5461 tcggtccaat atttattgga gcaatgttgt ttccggccac acgacaatgg gggatgaatt
     5521 ggattgggca aattatgaat tacgcagtaa cagttacttt ttatgtgtta cttggtacaa
     5581 ttcaaatcac attctttgaa gcgcatatgc tatccatgat agaaaccgac ctgataaaag
     5641 tggctgataa aggttcaatc agtcttgttg ttttcattcc aatagcattt atgtttattt
     5701 tatcaactat tgttttcttt gttgtagcgt tatctattcc gtctattgct tctgctttaa
     5761 ctggcggagc tacgattggt ggatatagcg gtattggtcg aaccatttca tctgcttcta
     5821 attcccttac caagcgatca ggttcattca aatctaataa tccaaataat caaataggat
     5881 acaacaaatc ttaaaataag gaataattat ggcttctaaa attatctggc aaaattcata
     5941 ctgttatttg actttatttg aaggtcaaat ggctgatttg catcagcgtt ccgaaactgt
     6001 tatcactggt gggggacatc atattggaag ctataccaac acgctaacac aaatttttat
     6061 tattgataaa caaggtaatc aatatacatt tggcacaatg aattgggata ttccaatgag
     6121 ggtcggcaat gatttaaaag tgataacaat ttgtcggaat aattgggcaa atggagaaat
     6181 cgttttagcg caaaatatta gcctaaatca gatttcatgg agttctgaac ggcttaaatc
     6241 agctatcaaa agagtttatt ttccattttt gaaatggggc ttggttactc tttttgtcat
     6301 tccatttatt ttgtactttg ttttatttgt gtttattcaa ccagaaccaa atagttttat
     6361 tgatacgata ttttcattac taatgaatgt gtgtcaatta ggcggattcg gcagcattat
     6421 ttacggctat catttttgct ggaggaaagc ctattcacaa gcaaataaat cacttgctat
     6481 taacttgtaa aaattttcaa tcaacaggca gtctgaaaac ttttcaggct gcctttttct
     6541 atgggacttt tatcatgaaa aaattgctca tcacactcac ggcaactgct ctactcgctg
     6601 cttgctcaca caatccaccc aagccttacg gcaaaccttt tccgattaac ccacagccct
     6661 ccataaccac agaaaaggct aatccatgaa aaccaattct aacaaaacac ccaaagcccc
     6721 taatccacaa caacagggac tgaaatttat tcaagaagcc aaagaatttc acaagtcaga
     6781 aattgaccgc gtacgtaaaa atagccgcat tgcatggcgt atttcaggcg tttgccttgt
     6841 gattacaggg ttggcagttg gcgcagtcgc tggcttgact ccactcaaaa ccacgcagcc
     6901 attcttggtt cgtgtggaca acaatactgg cgtaaccgat gtggttacca cactcaaaac
     6961 ggctgaaaaa acctacggcg aagtggtgga taaatattgg ttggcgcaat atgtgcgtta
     7021 ccgtgaaagc tatgattggc aaaccattca ggctacctac gatgcagcca tgttgttgag
     7081 cgacccgact attcaagcag aatttgccaa actctacaat aatccaacag ccgcgccaca
     7141 taaaattttg cgcgaccagt ataaagtgat tgtcaaagtg aatgccatta gcttcttggg
     7201 aaatgacgtt gctcaaattc gttttgaaaa acaaaccatt ccaaccaaca gcgtgaatca
     7261 gccattacca ccacagcgtt tcatcgctac cgttggttat gcctacaaaa atgaaccgca
     7321 agaagaaaaa gaccgtttaa tcaatccatt gggttttcag atgaccagct accgcgtaga
     7381 ccctgaaaac accccaaacc aataggataa catcatgaaa aataagttga tttatttcac
     7441 aatagcagcc ttgatgctgc cattttccgt tcaggctgcc agtattccca aaggttcgcc
     7501 caaagacaga cggattcaaa ccgccgttta tacccctgat gacgtttttg tggtgcgtac
     7561 caaagtcggt acagccacca ttattcaact ggaatctgat gagcggatta ttggcgaaag
     7621 cagcgcaatg gggattggcg atgccgaagc atggaaaatg accgctcgcg gacaaaacat
     7681 tttcttcaaa cctacggcag aacgccctga tactaacttg ctgattacca ccaacaagcg
     7741 aacttatgcg tttgatttga aatctaccaa tggcacaccg ccacctactt acattttgcg
     7801 gtttcattac cctgatactg aaaatgacaa acggcggata gaaactcaaa aacgtattcg
     7861 cgccgcagaa gtgtttggga aagttcaggc tgccccacca ttgggttata acaatgatta
     7921 ctggggacgt ggcaacaaaa atcttgcgcc aaccagtatt tatgataatg gacgatttac
     7981 ttatttccgc tttaataacg gtcgtgattt acccagcatc tacaaagtgt tagatgacag
     8041 cagcgaagct ctgttgaata cgcatatgga cggcgatacc gttgtaattc acgaaacagc
     8101 acaacatttt atcttgcgtt tgggcaatca agttttgggt attgaaaatc gtggtttcag
     8161 cagcaacggt caattcaatc aaaccggcac agatgacaat tcttccattc gtttaatcaa
     8221 aggggaataa ggtgttaaag aacattttta accgcaaaaa acaagaatcc aatttgcccg
     8281 agcctgatta tgagcagcct gaaaatgctg aaaacagcat tgaaactgat gaacccaagc
     8341 cattgagcga acaaatcaat gacgtgaatg tggaagctgg gttgccgttg gattcatcgg
     8401 gcggtaaaaa caatatgacc aaagtagcgg ttttggctgt tgcattggtc ggtgtgggca
     8461 ttgctgttgc aggtatgata gccttttcca gtggtggaga tgacgatgaa gcagttgccg
     8521 cgcaacaaca agaattggag cgcgtacaga aaacccaagc caaagatttt tctactgaaa
     8581 aactggattt tggacaagaa gaaattgccg catcagaacc tgtggctgca tcacaagctg
     8641 gaactgccga agcgcaaact gtaccgccga ttgaaaccac cgaagcacca ccacctacgg
     8701 aaaccgcgcc gccaccagct acgcagcctg aaaccgcaca aactgccgaa cccaaagaaa
     8761 caccacgcga ccgcaaatta cgcggcgatg tgttgattgg cgaaaaatca agcgatatgg
     8821 atagtacagc acaagcaagc gatacagaac aaggggcatt ccaagccaca tctttttctg
     8881 aaaagccgtc agcaacacaa gaaaattcgc aatcgcaaag cagttttgca tcacggttaa
     8941 atcccagcgt aacgccgagc atgaaagcgc aacgccgcgc tgattctacc tatttgctgg
     9001 caaaaggcac gaacattcca tgtacattgg acacgcaaat catcacaacc cacgcaggga
     9061 ttacgcgctg tttggttacc aaagacgtgt attcagccaa tggcaaagtg ctgttgttgg
     9121 aacgcggcac aaaaatcatc ggcgaacaaa acagcgcgat gttgcacggt caggcgcgtg
     9181 tgttcgtgtt gtggaatgaa gcagaaacgc ccaaaggcgt gaaagtttca ttggcaagcg
     9241 gtggcgcagg tcagttgggc gcatcggggc atggtgcgcg tgtgaaatat catttttggc
     9301 agcgttttgg cggcgcggtg ctgattagtt taatcggaga tttgggcgat tattacagca
     9361 accgttcaca aagcaacgga caaaatattt cttttgaaaa ttcgtccgaa tccgcacagg
     9421 aattggcaac cgaagcactc aaaaacagca tcaatattcc gccgaccggt ttcatcaatc
     9481 acggtacgct catcaatgtg atggtggcgc gcgatgtgga ttttggcggt gtatatgaag
     9541 ttgtcaaacc gtatgaaatc ttttaatgat gacaggcggc ctgtgttttc aggctgcttt
     9601 tttatggaga aaaatcatgc aacccaccca aaccattcaa gcggacacaa gcgcacgaaa
     9661 cgcgctagac cgcttgggta ttagcgatta tttgaatcgg gacggcgtta ccgaagtcat
     9721 catcaaccga cccaacgaaa tatttttaga aagttcatca ggttggcaat gtttccaaga
     9781 tgagcgatta aatttgaaag cattggaaca tctcgccaac acgctctgcg tattcaacag
     9841 taaacacatt tctaccgcca acccgattca ttccgtaacg ctgcctgacg gcgagcgcgg
     9901 acacatcatg attccgccgt cttgcgaaga caaaaccatt gtgtacgcct ttcgcaaacc
     9961 gtccaaaacc cgtttttctg ttgatgatta catcaataca gggagattga gcggctttcg
    10021 tgatagcgca cagtttggcg tttcaggcag cctgaaaacg gaaattgacg gccgccattt
    10081 tcgggatatg gcggacaaaa tgaatttacc gcccgatgta ccattggctg actggcaata
    10141 cgaaatgctt actcacaaag ctaatcgtga tttgaatgcg ttttttcggc tcgccattga
    10201 gcataaattg aatatttgca tggtgggcgg tacaggttca ggcaaaacga cttttaccaa
    10261 agcgatttcg gatttaattg acccgaccac gcgcatcatc acaattgaag acacgcacga
    10321 attggatttg cccaagcacc caaatcatgt ccacctgttt tataaagaac acatcacggc
    10381 aaaacaaatc atcgcagcct gtatgcgctt gaaacctgac cgcatttttt tgacggagct
    10441 gcgcggcgat gaagcgtggg attatctatc cgcgctcaat accggacacg ccggcggttt
    10501 gacttcggtt cacgccaatg acgcgcaatc ggtgttttac cgaatcgcgc aactggcgaa
    10561 agaatccgaa acagggcgca caatggacta tgaatttatt ctgaataccg tccgttctac
    10621 cattgatatt gtgtgctttt ttgaccgaac acgcatgact gaactgtatt ttgaccctgt
    10681 ggcaaaattc cgcgcattaa gcggaaggtc gtgattttta acgattcagg cagcctgaaa
    10741 atcatgtttc aggctgccta tttattttgg aagcacatga tgaataatcc aagtcgtatc
    10801 catgtattac agaaattaaa catcactaat ccacccacgc aatcaactcc taatatgtcg
    10861 gatttatcta accaagaagc aagattagcc gtattagcat gggctgaacg tacaaaaaca
    10921 ttggagtctc aacccaaacc ccttactcac ttggcgattc agacaatact ggctcgtcat
    10981 caacagaatt atccttaatc aatttatcca gtttttgcca aaaaccaaag tcatcacaac
    11041 caaaatcagt ctgaccgttg agataaaaat tatcaactat ttcaaccaat tcaataattt
    11101 gctcagtaga ttgattgtgt atgccgactt taactagcca taaagccaaa ttttcaaaaa
    11161 agcgtgattg ctgcttttgc tgctcacgca ataaagccac ttcttctgat aattttttgt
    11221 attccaagac aaaattatcc aaacctgttt ttttaaccat tactgtctcc atagaaaggt
    11281 aagatgatgt tttacaaccg cattcaaatc atgggcaatt tgggcaaaga ccccgaaatt
    11341 cgttattttg ctgacggcac accgtctgcc aaactgtccg tagcctatac cgaaaaatgg
    11401 cgcgacaaac aaactggcga accacgcgag cgcacagaat ggtttaccgt gctgctaacc
    11461 aaaaatcaag ccgaaaccgc cgccaaatat ctgcacaaag gcgacaccgt gcaagtggct
    11521 ggcaaaatgc aatcacgcca ctataccgac aaagacggtg cagagcgtac cgtttacgaa
    11581 atccacacca acgaattgct gataattaaa acgcgcaaat caaacgattt ggaacaggct
    11641 gatgcccctg aaaattaacg tgaaataagg agaatcctat gtccaaaacc ctaatcatcg
    11701 ccgaaaaacc cgatatggca aaatccattg ccgccgcgct cggtattccc aaagccaaag
    11761 gttgctacga aaacgaccgt ttcattgtca gtaactgtat cggacatttg attgagcttg
    11821 atgttcccaa atggcgcgac aagaacgcat cattgcctgt cattccgcaa actttttccc
    11881 tgaaaatcaa agaaaccacc gcgcaacact atcagttttt gcgtggttta ttgcatcgtg
    11941 cggacgtgtc tatgctcgtg aatgcctgtg atgccgaccg tgcaggagaa catattttcc
    12001 gattggttta tcagcattca ggctgcctga aacctgtgaa acgtatgtgg attcaatcca
    12061 ctacgcaaca aggcttacgc caagcgtttg aaaacctgaa aaacggcgca gattacgacc
    12121 atctcgctgc tgccgaacaa tgccgcgccg aagccgattg gctgctcggc atgaacggtt
    12181 ctcgcgcatt gggcaatcag tttggacgcg taatgacccc cacgctggca atggtagttg
    12241 atgcgtatct tgccaatcaa aacttcaaag cggaaaacta ctgggaaata tcggtacaat
    12301 tttctattaa atcaggaagt tataatgcaa aattattgga cgaaaacgac aaaatcgccc
    12361 gttttgccga tgacaaaacc gcgcaggctg cctgtgaaca aatccgcaac caaaacacat
    12421 ttgccgtgaa tgatgttgcc gaaactgaat tttctcacgc tccatatttg tttgatgcca
    12481 atgaattgca aaaaatcgcc aacaaaaaac tcggttttgc tgccgataaa accttgtcta
    12541 tcatgcaaag tttgtacgaa aagcacaaag ttttgactta tccgcgctct gatttcaatg
    12601 ctttgcctga tgattacttg ccgactttaa ccgccacgat ttcgggatta ggcgcgttgg
    12661 cggaatacgg taacatcgtg aacgatatac aagcccatga tttgattaaa cacaatccgc
    12721 gtgtttttga cgacaagcgc atcggctcac actatgcgat tgtgccaact ggatttatcg
    12781 ccaccgccga cggcgaacac gctttatccg attgtaagcc ggatgtactg aaaaataccc
    12841 tttcggctga tgaatatgcc gtgtttcatt tggtggcttt gcggacattg gcagcgttat
    12901 atcctgcggc agaatatggt gttaccacgc gcaccaccgt ttcgggcagc ctgaaatttc
    12961 gtaccacagg gcgcgtactg ttgaaacaag gctggttaag cgtgtatgcc ggcattgatg
    13021 ataataacgg gtcgtctgaa aacgtgccgt ctgaaaaatt accacctttg tcatctgatg
    13081 aaatggcgca accggacaca atttcatcaa aaaaattgac aaccaaagca ccgccgttga
    13141 tgacagaaag ccgtttaatc caagccatga aaaccgcagg gcgcgacctt gacgacaaac
    13201 atttagctga tttaatgaaa gaaatcaaag gaattggcac atcagccaca cgcgccccga
    13261 ccattaaaaa gctgaaagag ccgaccgcgc aaggcgatac cgcttatttg tctttggata
    13321 aaaataagct cattcccagc cagcgtgcgc ttgatgcgat tgctcatatt cgccgctttt
    13381 ttcctgaaac tgccgacccg attttaaccg ccgaatggga agataaattg caccaaatag
    13441 aacacggtca atttccgcct aatcaatttc gcaccgaaat tcatcaattc gtgcgtgatt
    13501 ttgtggcaac ggttttgccg catcgcccca gtcatcacag cacagaaatc ctgtgcgcgt
    13561 gtccaaattg tgattcaggc agcctgaaag aacaaaaatt ttcgtggaat tgtgaatgcg
    13621 gtttcaaaat cagcaaagaa cagcgcggtc gggcgatgtc ggctagtgaa ttgcgccaac
    13681 tcatcagcac aggtcaaact gatgtcttgc aaaattttgt ttccaaagaa aaagggacaa
    13741 aatatgccgc catgctgaaa ttggaacgcg atgattcagg cagcctgaaa atcgccgttg
    13801 cctttcccga gcgcaaaacg gaagtctgtc cctgttgttc aggcagcctg aacgataagg
    13861 gcaaactcta cgaatgcgcg tgtgggttca aattgtggaa aacgcaattt ggcaaaacac
    13921 tgacggaaaa gcagcttaaa tctttgattg ataatggtga aacgggttta attaaaggtt
    13981 ttcacaaaaa agacggtggc acgtttgatg ccgttttgtg cgtggacaag gcgaacaaga
    14041 agattggttt tccgccgcgt aaataatcag ttttcaggca gcttggattt tcaggctgcc
    14101 tgaatttttt ataggaatca tcaaatggat aaggacaatt taagtgatat gacacgcaac
    14161 attatagggt cgtggcggct agaaaattta gaaatcagca cagagaccat tgctgatatg
    14221 gaagcgttag cacacggcaa tttaacgcct gaaatgggta ttgagcgtgt gagacaacgt
    14281 attttacagg ataacggtca tgaaaaataa gatattagcc cctattctat ttattttgtt
    14341 taccctgccg ctcgctgggg ctggcggtgg atttgtcggc agtatgctgt ttgtacgctg
    14401 gcaaaaattg aatagcaaac cgtccgtatt gttgctctat gaatacgcac aacacatgga
    14461 caaaatgcca gctaaattac tgatgccatt caaggttagc gttacgattt ctttactcat
    14521 cagcatttta cctgcgctga ttattttggc tgcggtattt gccaaaccga aacgcgaact
    14581 gcacggctct tcacgctttg ccaacgcggt tgaagtgcaa caagctgggc tgctgaaacc
    14641 gaaatacgac cccaaaaccg acaaacccga tttgttgatt ggcaaataca aaaacaaata
    14701 tttacgctgg gccggtaatg aatttctgta tttggccgcg ccgacacgct cgggcaaagg
    14761 cgtaggtgtc gtgattccaa actgcttgca ttatcgtgat tctatggtgg tgtacgatcc
    14821 aaaattggaa aacttcatca tcacaggcgg tttcaggcag caaaaattgg gacagcaggt
    14881 tttcctattc aatcccggcg gacgtatgcc cgaacatgaa cgcaatccga tgttgccgct
    14941 cgttagccac cgttggaatc cgatgaccta cattcgccgt gatgcacgat acagctacaa
    15001 agatttgtcc aatatggcag ccattttgtt gcctaaacct gccaaagaca acggcagcgg
    15061 cacatttttt gtggaaagtg cccgaaaatt gtttgtcggt ttgggtttgt acatggttga
    15121 aaccgaacgt gaacgcgatt tgacggatta cacgcaacgc accacattgg caaatctatt
    15181 ccgtttaact gccccgactg acggcaaacc gctcaatgaa tggctgctgg ctgaaatcgc
    15241 tgaacgcgat aacgaccctg aaaaaacgcc gttatccagt caatgcaaaa cgctgttgct
    15301 cggttttgcc aacggcagcc cgaaaacagg cgcggacatt ttggcatcgc tgaccgcgcc
    15361 gcttggcatt ttccttgacc ctgtttgtga agcggcaact tccggcgatg acttccgtct
    15421 tgatgaactc cgcaaaaagc gcatgacgat ttacatcggc gttgtgccga ctgaaacgga
    15481 aacattcagc cgcttaacca atttgctgtt ctcgcaaatt attgacgtga acgtgcaaca
    15541 aggtttgccg caggacaaca aagatttgct gaaatatcaa tgcttgctgc ttatggacga
    15601 atttaccgca ttgggcgtga ttccggccgt tcaacatggc gtgtcgtaca ttgcaggcta
    15661 tgaattgcgc ttgctcatca ttattcaagc tccgtcccaa gtagaagcga tttacggacg
    15721 tgaaaacatg gaaacctttt tcacgaactt tacttgtcgg atcttctaca cgccgcgcgt
    15781 ccaaaaagat gccgaagaat acagtaaatc aatcggttat gaaacattta aagcacgttc
    15841 tacatcgcgt tcgcaaggca aaggcggcag ccgcagtacc agcatcagcg accagcaacg
    15901 tgccgtgatg aatcccgatg aaatcaagat gatgccgaac tcggactgca tcatcaattt
    15961 gggcagcagt cgtgccattt acgcgcaaaa aatcgtgtat tggcaagatg aggtgttcaa
    16021 agtccgtgcc aatttgccgc gccccgaagt acccaatctt gaaatcaaaa cccaccgcag
    16081 ccggccgaaa tctacggccg cccaaactac caaaaccgag cctgttcccg atgaaaagct
    16141 gcctgaaacc gatttcaaaa cctgttccaa cgctgatgat ttattcatgg caatggttca
    16201 atcattggtg cgccccgata gtccgcctga atttatcaag gaaattgtgg aagctatggc
    16261 gcaacaatgg ggcaaagatt cggaacccat tttggcaaaa atgttgactt aaaccaatct
    16321 aaaacaaggc agtctgaaag caacggcttc tgcgaagcta aaagattttc aggctgccta
    16381 aattttgggg agacaaaaaa atgagtgatt acaatgaaat tcgcaatgcg ctaaatcatc
    16441 ttgatgcaaa tgaccgtgat ttttgggtca aaatgggtgc tgccgtcaaa gatgaattag
    16501 gagaagacgg ttttgaattg tgggacgaat ggtcgcgcca ttcagaaaac tacaaatcca
    16561 atgatgccaa agccgtttgg aaatccttga aacaaggaca tattcacatt ggcacattgt
    16621 tttaccatgc gcgtgaaaat ggctatcacc ccaacaaacc ctacacgcca cccagtgccg
    16681 aagaattagc caaacgcgct gccgaaaccc aagaaaaaca acgtgccgaa gccgagcgca
    16741 tcgcccaagc tcacgccaaa gcggaaaaag cagcgtatgg catttggaaa aatgccagtc
    16801 ctgccgaccc taatcatgcc tacgcgcaaa ccaaaggatt agggctgaat acggacgtga
    16861 tgagcggtat tcgtcaaaat gaatatcaag gtaaaaagca gttgattatc cctttgtatt
    16921 caaaaaataa attggtcaat gtacaaacca ttgatgaaga cggtcataag aaatttttga
    16981 atggcgggca gaaacaaggt gcgtacaccg ttattggcga tttaaaacag agccaaaacg
    17041 gtatcttttt ggcagaagga tttgcaaccg catctagcgt gtatcaagcc actaagaaac
    17101 ctgtggtagt cgcatttgat gcaggtaatt tgaaagccgt ttcagaaaaa ttaataaatg
    17161 ttctgcctga aaacgtgcct gtttattttg ctgccgataa cgacccaagc cacacaggca
    17221 aacacaaagc gcaagctgcc gccgcaatat ggggcgaacg tgcaaaaatc atcttgcctg
    17281 aatttacacc gaaacaaatt catgaattta aagaggatta tggaacagaa aaaataccca
    17341 ccgattttaa cgatttgcac aaactggccg gtattgaagc agtgcgtgaa acattcgaca
    17401 ttgagcctga accttatatc attgaaacag aaacagatat tgaatttaat aggcagcctg
    17461 aaagtaatcc gcaggaaatt cgtactgctt ggaatgattt tccaccggtt attagcaacg
    17521 gcaaattagg cgatttaaaa aatgaacctg aatatgaggc agcgaaagcc ggcgatgtga
    17581 tgcagtccgc gctattggta gataaactgc tgaaagatga aaccattcag gaaattaaaa
    17641 acttgattgg agatagcaag cctattatcg taccgattca atccgaagaa gcaagcggaa
    17701 aaaaccgtat tccacaagct atggcttttg cgttatcaga aaaattaggt ttacaagtag
    17761 ataccgatat tgtgcaaagt aatacagcaa agcgaaccaa tgcaaaaatt gaccatcgtt
    17821 ttgtttcgcc acctgaattt acaggcacag tcaaggccgg acaaccttat ttgattgttg
    17881 atgatacttt aaccgtaggc ggtacgattg ctgcgctgaa aggttatatt gaaaataacg
    17941 ggggtaaggt tatcggctct acggttatga ctgctcatga acaaaaagca gatattgtga
    18001 ttaagccaaa tatgttacaa tctttgcaac aaaaacaagg attaaacgaa tattggcgca
    18061 atgaattcgg ctacagtgtg gacaaattaa cccaacagga agccggacat ttacgcaaac
    18121 cgacattaga gcaaattcaa gaacggattg aagaagccaa aattgccaaa caagcatcaa
    18181 acatttcaga aaacaaggaa ttacatcatg aaagaacaac agaaacccaa tttgctgaaa
    18241 cccgaaattc agaagtatca aacgtatttg accgagcagg aagtgaacga attgagagca
    18301 tacggcaagg agaccggaaa atttctcaaa gcgtttttca gcaagaacaa acaactgagc
    18361 aaatcgtaaa tcaacccgcg caatcagcca catcagaaaa agatgtggct gatttttcat
    18421 ttaaggagac gaaaatggat aacttacaag aacacggcaa acccgatgtg ccacgcgaag
    18481 aactactgaa aaacaattac tggcgtgtcc aaaatggcta tgaccctttg tctgaaaatg
    18541 accaaaagcc cgagcaaaca acaacactcg ggcaaaccac ctactctgct gacaatccct
    18601 atgcacaatg ggcagaacat gatgatgaag ttcgtcataa tgcagacagt tgggaaatgc
    18661 ggacaccttt aaccaatgaa agattggctc acttcaatca acaagccgag caatacggtt
    18721 ttactacata tattgaaaac cgtcatgccg agttaggcgt ttttcaggcg gataaatgga
    18781 ccaatgaaac ctatcatgta gaaattgacg gcaaattcaa aaatcacggc tcaatggctg
    18841 cctatgaaga tgctcgtgat tttacgatgt cttttcatga aaatgatgaa attgtccact
    18901 atgtttacac caatcatctt gatgatgcac ttcaacaagc caatgacttt ttacaaaatc
    18961 cagcagaatg gattaaagag caagatttga aaatgcaagc agaagcaatg cagcctgaaa
    19021 atgcaccgcc tgaatacgat tatgtgcctg aatattcatc tgatgataac gaagaacctc
    19081 agcctgaaat gttcaacggc attgaatacg gtggtttcca acgtgaaatt aagcaacctg
    19141 aaaaacagcc tgaaaaacag cccgaatcac aacccgatgt agaagagcct gaattaccac
    19201 gctccgaagc attggaacat cggcccattt tggatttgac ttacgaacgt ccacccaaac
    19261 atctgcaatc gcgctacatc gttgcgaaaa atgggcaata tttgtctgcc gacaatcaca
    19321 ccaccgtttt gtttgaagac aaaggcaacg cacttaaaac cgccaaatct gaccaacaaa
    19381 ccattcagga tatgttggaa gttgccaaag ccaaaggttg gaacagcatc aaaatttcag
    19441 gcagccctga atttaaatcg caaatgtacg ttgccgctga aagtcagggc atcaaaacca
    19501 caggttacaa acccactcct gcggatttgg caatggtaga aaaattgcgt gaagagcgtt
    19561 ccttgaatgg tattcaaagc actcataaac ccgaacttga ccctaagcag cctgaaaatg
    19621 cgcctgctca tcaatccgat gccgccacgt tatcggctgc tgacaagctc aaaacacaaa
    19681 gcgagatgcc cgatgaacgc caaatcaatt catcgccgat tgcagatacc gaagccgcgc
    19741 ccaaagtgga tagtttgggc aacaaaacca ttccgtccga agtgttacgc agtacgcatg
    19801 aaatgactgc cgccgcgtta tcgcccgaag tagaaacctc caaatcgact tatgaccaaa
    19861 aagccaagca gttaagcgag ccgaaccaag cacggctcaa agcctacgag ctcaacacga
    19921 tggacgcatt gcgtaatgtt cagggcgatg cgcgtaacca tgctttgcgg aatttctacg
    19981 aacacaccgc caaagtaatg aacggcacgg aattgaactt gccacaacca atgcaaaacc
    20041 gcgcccccac tcatcatcag gcacatgaac cgcaacagtc caacagccaa gattttgaaa
    20101 tggaacgctg atttttttca acaaaaagcc ctgtttagca ttgcgctaaa cagggcttta
    20161 ttttgataaa aggaataatg atgaaacaac aagaaatccg tgtttttggt ttaagtcatg
    20221 atgtcatcaa tcagcttcaa ctaatggcat tagaacgcta tggtaaagcc agcgcatctt
    20281 taatgttacg aaaactggca gagaaacaaa tcaggcagcc tgaaaatatt cctactgaaa
    20341 attatcaaga aaacaagcaa aatcaacgct taactttacg cctgccacca cagcagcaac
    20401 attacctaaa ccaaaaagcc caactgcaat acagtagttt aaacgatgta gtacgcgata
    20461 ttatcgctga gtacattacg caaaatcctg tattgagtaa tgacgaagta caggctctat
    20521 atcagtctaa ttaccaactt ttgcgtttgg ggcgcaacat caatcaaatt gcgcgacaac
    20581 tcaatagcat tacacctaat tctttgacaa gtcagcaatt aactgaatta tcagcatttt
    20641 taaagcaaca cgctgaaaca gtcggtaaag ttttacgcaa gcaaaataaa ccgttcaaat
    20701 atcgtcctat tgaaaatatt caaaatgaaa cagatagata gaagaattga taactggttt
    20761 cttggctaca cacgcagcgt tcacaccaaa tcagacagcc tgaattttgg tagtcgccac
    20821 tcgtccaaac cactcaaaac aggtacaggt ttaaccaatc tacgtgtcgc cgcacaaaaa
    20881 aagcccgaag tcatggtaaa aattcccaaa cgccacagca aaaattccaa aggcatgaaa
    20941 gggatacgca accatgcgga ttatgtttca cgcaatggcg aaattgcact ggaaaaccaa
    21001 gacggcgaaa tttttcatgg taaacgagcg gtaaaagaat ttttgaacga ctggcaaagc
    21061 cattgcggta tcgccgatga gacacgacac aaagaagccc tgaatattgt cttgtccatg
    21121 cccaaagaaa cgccacccga tgccctatta caggcagcgc gtgattttgc cgcagagcaa
    21181 tttcaaggac accaatattt tctaggcttg caccacaaca gccatgaccc tgacgaaccc
    21241 gaacacccac acgttcatct atgcgtactg atgcgagatg aacgcggtca gcgactcaat
    21301 ccacgcaaaa atgatttgtt tgagtggcgt gtccgttttg ccgaaaagtt gcgtgaaaat
    21361 ggcgttgatt gcgccgccac caagcgcgta catcgtggta aaacccaaaa gcttgaaaac
    21421 agcacaatcc gcgccatgcg tgaacgtgga caaagcagca aagcagagtt gcagcagaaa
    21481 ctagccattt cacaagctat tttgtcaggg aaacgccctg aacacccatt tttgaatcaa
    21541 gcggacaaaa cgcgccaaaa gattgttgca gaatacaaaa tgattgcgcg tgaactgtac
    21601 atgaatggct acaaaaccga agcgaaagcc atgagcaaat ttgcccaaga agtgtctgaa
    21661 aaaggattta ccacccaagc gcaacaggaa tttgacaagg ttcagataca aatcaggcag
    21721 cctgaaaaat cgcccgaccg ctcacacgat aatgaaatgg aacgctgaaa tattttcagg
    21781 ctgcctgttt caatttgaca acgcaccaag aaaaacccta tgctgatagc ctattttttc
    21841 aaccctatac ggagtttcaa aaatgaaacg catcacttta tcggttcttg ttttgatgtt
    21901 ggcagcgtgt ggcggtaaaa acacagatga ttatgtcggt gtttggcaac gtgatgatgc
    21961 taaatttttg caatttgtag aaatcaaaaa agacggtggc aattacctat tttcacaagg
    22021 agaacaaaac tatacttttt ccgaaaagaa tggggaattg gtgctgaata cagtctttgg
    22081 agaaatcccg ttcaagctgt ctgacgatag aaaaacgttg tcagctaatt tgtattcaag
    22141 cggcagcaac cgttttttta aagtggaaga tgaatcttgt aaacagttga ttgcagaatt
    22201 gcaaactgca caagaaaaat taccactgaa cgcatggagt aatgaattta aacaagcaag
    22261 cgcaaatatc aaagcaatta gcgaaaaata catcagtcaa tgcagtaaaa aataaacgca
    22321 tcaaaatgaa aaaacgccta tggctgcctg aaaaaattca ggtggtcgta ggcgtttttg
    22381 gtacaattta ataaactgtt gaaaaaggaa taatcatgcc tttacatatc ccccccaacc
    22441 agcaaacagc gttacatcag tgatatggtc gcgctcaata ttcgcagtcc acaaggtaca
    22501 ggcgattggc attcagccgc aacattcgcc agtgcgctac ctgaacgtgt ttttgtttat
    22561 ggcgataatc aaccatacaa taccaatcaa ctattaggcg caaaaggtgt tattgacggt
    22621 acaaaacgat tgaatagcat gggtattttt cctgaaaata gcccagtttt cattgccgac
    22681 cacccacgcg cctgttttga tttgctttac accgttgttt tgcaaaaagg cgcaatcaat
    22741 agcgtaattt tagatgactg gtttccagca cccgaagaca agcgagccgt atacgcgctg
    22801 attcaggctg ccgaacctta tttaaatcat caacaaaaag aaagattagc cacatggaaa
    22861 cagaaaaatc caatcaactg atttcataag aagagcggaa tgccgcccat gaaaagttaa
    22921 tggttgccat tctgcaaaat ctttctgata ctcctttaaa tcaatcagag acaatcccaa
    22981 catttttctc atgatgaacc cgaaatagaa cgctaaaaag cctacggctg cctgaaaaat
    23041 atcaggtagc cgtaggtttt ttttacgcct gaaaattgcc cctactctct ctttgataaa
    23101 ttattcttta tgaaaaaaat agccgcatcg cggcgtttcc gcacgagcgg aatgtaaaaa
    23161 aacggcgatt tagtccgttt tttggcacac cgtgcctatc ctgcgttcta gtttttaaat
    23221 aggtttaagc ggtttcatag ggggtaaaaa cctaaaacaa gcaaaaatat tgtctgttat
    23281 ttgctaatat gttgcttacc agttgcatta tgttgcttac tggttacaat atattgctta
    23341 ttatttgtga atgcgttgct tgattgtttc aatatattgt ttactacttg ccaattcttt
    23401 tcctacaagt tgcaatatgt tactttctgg ttacaatacg ttgcttaata gttgcaatat
    23461 attgtttact ggttgcataa tcattttttt caagaaaaag gatattctcg ccatgaacga
    23521 cattgaaact tttttaaatc agcgaaacca cgccgcaaaa tcggacaagg tttccgttct
    23581 cgaaccgtac aaagatgaaa tttttcagtt gaagaatatg ggctatgcag aaaaaatcat
    23641 cgttgatttt ttatctgaaa tgaaaggcgt aaatgtcagc cgtcaagcgg ttaatcgatt
    23701 catccgctca cgttcggctc aaatgcacaa cacggcgcaa aacaagccga ttatggcaac
    23761 agatagcgtt acaatggctc aaaccgctca agcggctgaa acagccaaaa atcaagcgga
    23821 aaatacacct gcacggcgtg gtttgcgcaa acccacaccc actaagaaat ttaactggga
    23881 agagaaaccc gaccccgaaa cactcaaata attctttctt gaaaaacaaa acagcctgaa
    23941 aaccacatgg attttcaggc tgaaattatt tggattgttt aatagtggaa tagacggtaa
    24001 ggcagttaag acttcacaag acttgtgaaa tcagaccact gctcaatgcg gaacgcccga
    24061 atatcgcgga cagaagacgg aaaccaaggc agagctttta gctcgttgat ggctgaaaac
    24121 aggttagcca tatcttcgtt ttggcaggtg tacaaacctc cctgaacgcg ggtaaagccg
    24181 aatttccgca gggtgtatcc aatatccgcg taggcttggg aaatgccttt cggatggttt
    24241 tgagcggtat cggcaaccac caaatcaaaa gaaatcgcgt acattaggca gcctttgggt
    24301 cttgaacgat gtgggacagt ttgtattgcg tgtgttcgcc gctttccggc agttcaatat
    24361 ttacgagcca atcgccgtcc ggaagctgtt cggcaggcgt gccgacgatg tacatcacgc
    24421 cgtaatcgcc aaaggtgcgg attgaaccgg tggggatggt atttgctgtg gtctgcatgg
    24481 ttagtcctcc tgttgcgttt cagacggcat aggtttgccg cgatactacc aagtggcgcg
    24541 ggaattaaat aagtacaaaa ataagtacgt attttagagt gaaccatgcc gtcatgcaag
    24601 gtagcccgaa atcattaagt tcaacacttt ttcttgataa atcaaataat taaattcaaa
    24661 cgcatatctc atttcgcaac agccccaaac cccaattcaa aaaaaatcaa aaagacgcga
    24721 ggaaggcgta tctgagcaat attgccaagg tgttaggggt taccctaccc gaagccctaa
    24781 aacgcaaaaa acgccgcagc gcgccaaaaa ctggcatcga tgcggcggat gttggagagc
    24841 aaaggcggtt aagggtattt cctgcttcca tgaagcaccc tcaaaatcct aatttcgtcc
    24901 aatttgacct gatagaccaa aatgtagttt gggtgagcta caaattcccg tgttcctgct
    24961 acacgacctt ttcgccccat gtatggcatc tgtccgattt tttcggcagt ttcaaacaaa
    25021 tgctcagaca aattaactgc tgcaacgggg ctatcgtctg cgatgaaatc cgttatctca
    25081 ttcaaatctt cattggcttc tgttgaccaa ttaatttgca tttctcagcc tttcagccat
    25141 ttttgcccgc attttggcga cgacttcatc atgtggaata gctggcttgt cgctgtttag
    25201 ggctgcgttc actttagcgc gaaaccattc atcataatcg gcggcatctg ttttgtttga
    25261 aaactcgtaa acaagcgggt cgtcatgaag ttttaatgcg gtgttcatgc aatcttctcc
    25321 tgttggttgg ttgtttcaga cggcataggt ttgccacgat actaccaagt ggcgcgggaa
    25381 ttaaataagt acaaaaataa gtacgtattt tagagtgaac catgccgtca tgcaaggtag
    25441 cccgaaatca ttaagttcaa cacttcttct tgataaatca aataattaaa ttcaaacgca
    25501 tatctcattt cgcaacagcc ccaaacccca attcaaaaaa atcaaaaaaa cacactgctc
    25561 ggcgcagccg aataaaattt ctttgagagt aatctttgtt ttgtaatctt tgtatatagc
    25621 gtgtcgattt ggcgcaatct aatctgcggc ttgtcagcaa agtagtctgc caaaaatcag
    25681 caaacataag tttttaaata acttttttct tgataaaaag gccgcatgaa accgcgctgc
    25741 aacgccaaat tctgactaaa aaacaggcaa atctaattat cgggcgaaaa aaaacacccc
    25801 aaagggtgca aatccatttt cagacggcat gaaagcccga aaaatgcgcc aaaatcgcgc
    25861 aaaatttaat gtatagacgt gtttaagcac agcaagctgt ccacttgctg ttatttttta
    25921 ctcaacccta gattttttag caggtttaaa cgccctactg tgtttaagtc tcgcggcttt
    25981 ttagattgtt gccgttttcg ctcggcttcc tgctgcttca taaacacctc gtattcgact
    26041 gcggaactgt ttggagcggg ggcgtggctc ggagcggatt tatcggggcg gtaaaagccg
    26101 gcccagcctg aaatcagcga tttttgcatc acgtttttca aatcaaattt ttgtttgcgc
    26161 aaatcggtta gctgccgaat aagctgtttc ttttgctctg cgacaagtga ttgtttagat
    26221 ttacttttgc tttcgcgcat tttgagaaat tcattccatt catgcaacgg gatgaatcgg
    26281 gggatgtgat aaccgtcttt atcggtatgg aatgccgact gaaattcagg cttcgacata
    26341 ccgcgaactt tcgtataaat cagttcaagc agtttttcac ggcaaagacg gtacgccatt
    26401 ttgccaaaca cgccggcgcg tttgtactca accaagccca tttccaatag gcattcacgc
    26461 gctttttcat atttgtggcg tgttaatttg agttcgttgg cgaaatcttc ggcggttttg
    26521 gtaatccagc cttgtttttt gggacggtgg ttgtccagcc attcagacca gccgaacagg
    26581 tttgccagca caagcgcggc ttcgctgttt tgtccgcaca acgcaaaaaa ctccacatat
    26641 attgctgtga agttgggcgt gagatgttgg cgaatgtcgg cggttagcat catgatttta
    26701 gctttttaat caaataaatc aggacgtaaa tctttacgcg tgatttgtga atgagttaga
    26761 tgttctattt taattgcggt ttcaggcgga attttatttc tgccttgaat ccagctatta
    26821 acggttgagc gttgtatgcc aagtacgcga gctaatgctg cctgactgcc agcaaattca
    26881 gccgctaatt tgactgcatt tcgcattttg aaaatccttg tagtccattt
//
LOCUS       DAXFZR010000018        26410 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000018 DAXFZR010000000
VERSION     DAXFZR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26410
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.018"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(134..625)
                     /gene="dtd"
                     /locus_tag="ACFJAS_000667"
     CDS             complement(134..625)
                     /gene="dtd"
                     /locus_tag="ACFJAS_000667"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC3981980.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(699..1457)
                     /locus_tag="ACFJAS_000668"
     CDS             complement(699..1457)
                     /locus_tag="ACFJAS_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3981981.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(1527..2393)
                     /locus_tag="ACFJAS_000669"
     CDS             complement(1527..2393)
                     /locus_tag="ACFJAS_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC3981982.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(2442..2720)
                     /locus_tag="ACFJAS_000670"
     CDS             complement(2442..2720)
                     /locus_tag="ACFJAS_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC3981983.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(2720..3010)
                     /locus_tag="ACFJAS_000671"
     CDS             complement(2720..3010)
                     /locus_tag="ACFJAS_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC3981984.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(3013..3543)
                     /locus_tag="ACFJAS_000672"
     CDS             complement(3013..3543)
                     /locus_tag="ACFJAS_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC3981985.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            3851..6167
                     /gene="tspA"
                     /locus_tag="ACFJAS_000673"
                     /pseudo
     CDS             3851..6167
                     /gene="tspA"
                     /locus_tag="ACFJAS_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
     gene            complement(6340..6757)
                     /gene="gloA"
                     /locus_tag="ACFJAS_000674"
                     /pseudo
     CDS             complement(6340..6757)
                     /gene="gloA"
                     /locus_tag="ACFJAS_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
     gene            complement(6782..7951)
                     /gene="lapB"
                     /locus_tag="ACFJAS_000675"
     CDS             complement(6782..7951)
                     /gene="lapB"
                     /locus_tag="ACFJAS_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC3981986.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(7962..8276)
                     /locus_tag="ACFJAS_000676"
     CDS             complement(7962..8276)
                     /locus_tag="ACFJAS_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC3981987.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSANLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            8586..9584
                     /gene="ilvE"
                     /locus_tag="ACFJAS_000677"
     CDS             8586..9584
                     /gene="ilvE"
                     /locus_tag="ACFJAS_000677"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC3981988.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(9830..10615)
                     /gene="fabI"
                     /locus_tag="ACFJAS_000678"
     CDS             complement(9830..10615)
                     /gene="fabI"
                     /locus_tag="ACFJAS_000678"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC3981989.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(10770..11591)
                     /gene="dapD"
                     /locus_tag="ACFJAS_000679"
     CDS             complement(10770..11591)
                     /gene="dapD"
                     /locus_tag="ACFJAS_000679"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC3981990.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(11801..13444)
                     /gene="pgi"
                     /locus_tag="ACFJAS_000680"
     CDS             complement(11801..13444)
                     /gene="pgi"
                     /locus_tag="ACFJAS_000680"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC3981991.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            13954..15186
                     /gene="pilG"
                     /locus_tag="ACFJAS_000681"
     CDS             13954..15186
                     /gene="pilG"
                     /locus_tag="ACFJAS_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC3981992.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            15260..16120
                     /locus_tag="ACFJAS_000682"
     CDS             15260..16120
                     /locus_tag="ACFJAS_000682"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC3981993.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            16117..16749
                     /gene="coaE"
                     /locus_tag="ACFJAS_000683"
     CDS             16117..16749
                     /gene="coaE"
                     /locus_tag="ACFJAS_000683"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC3981994.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLILSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            16742..16951
                     /locus_tag="ACFJAS_000684"
     CDS             16742..16951
                     /locus_tag="ACFJAS_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC3981995.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(17037..18713)
                     /gene="pilB"
                     /locus_tag="ACFJAS_000685"
     CDS             complement(17037..18713)
                     /gene="pilB"
                     /locus_tag="ACFJAS_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC3981996.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(18959..19030)
                     /locus_tag="ACFJAS_000686"
     tRNA            complement(18959..19030)
                     /locus_tag="ACFJAS_000686"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18995..18997),aa:Arg,seq:cct)
     gene            complement(19051..19497)
                     /locus_tag="ACFJAS_000687"
     CDS             complement(19051..19497)
                     /locus_tag="ACFJAS_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC3981997.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(19501..20475)
                     /locus_tag="ACFJAS_000688"
     CDS             complement(19501..20475)
                     /locus_tag="ACFJAS_000688"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC3981998.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMMQLAKESLVRVS"
     gene            20699..21007
                     /gene="rplU"
                     /locus_tag="ACFJAS_000689"
     CDS             20699..21007
                     /gene="rplU"
                     /locus_tag="ACFJAS_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC3981999.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            21032..21304
                     /gene="rpmA"
                     /locus_tag="ACFJAS_000690"
     CDS             21032..21304
                     /gene="rpmA"
                     /locus_tag="ACFJAS_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC3982000.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            21539..22723
                     /gene="ubiM"
                     /locus_tag="ACFJAS_000691"
     CDS             21539..22723
                     /gene="ubiM"
                     /locus_tag="ACFJAS_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC3982001.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(22912..23067)
                     /gene="rpmG"
                     /locus_tag="ACFJAS_000692"
     CDS             complement(22912..23067)
                     /gene="rpmG"
                     /locus_tag="ACFJAS_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC3982002.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(23099..23332)
                     /gene="rpmB"
                     /locus_tag="ACFJAS_000693"
     CDS             complement(23099..23332)
                     /gene="rpmB"
                     /locus_tag="ACFJAS_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC3982003.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(23582..25105)
                     /gene="farB"
                     /locus_tag="ACFJAS_000694"
     CDS             complement(23582..25105)
                     /gene="farB"
                     /locus_tag="ACFJAS_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC3982004.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(25129..26283)
                     /gene="farA"
                     /locus_tag="ACFJAS_000695"
     CDS             complement(25129..26283)
                     /gene="farA"
                     /locus_tag="ACFJAS_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC3982005.1"
                     /translation="MEAHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRYPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         6419 a         7478 c         6574 g         5939 t
ORIGIN      
        1 tcaaatattt gatttatata ttaatttaat taaatattac atgaccctgc ataggggcga
       61 cggaaaggcg gcgcggggga agggtgtgtg tattgtcgtt cattgtcgat atttcatcat
      121 tgcggactca atttcaatcc ggaacaacct tcatttttgg ggaaatccgc gtcatgaaag
      181 agtccagcag tatggttacc ggcccgtcgt tgcagagcga cacctgcata tgcgtgcgga
      241 aacgccctgt ttcgacatga atcccgtgtc cgcgtaacag ctccgccgtt cgcaggtaaa
      301 gccgttgcgc ctgttctgcc ggcgcggctt gggaaaacga aggccgccgc ccgcttgccg
      361 cgtcggcata aagcgtaaac tgcgacacca gcagcaccgc gccgccgaca tctttcaaag
      421 acaggttcag cttgcccgct tcgtcttcaa acacgcgcaa atgtgcgatt ttgtcggcga
      481 tatagcgtgc atctttttct gtgtcgctat gcgttacacc gagtaacacg acaaacccgc
      541 cgtcgatttt gccgcaggtt tccgtgccgg cttcggacac gacatccacc tttgcaccta
      601 ccgttttctg tatgaccgca cgcataagcc gtcctcctta cctgaacgtg caatgccgtc
      661 tgaaaaggct tcagacggca tcggtatcgg aaatgccatt acggtttgac accgttttct
      721 tccaaaaggg catcgatttt caagcgggct ttttcctgcc tcaaaccttg ttccaactgg
      781 tttctgacca actcgaaagg ctgcgcgtcg gggtttttcc cgaccgcgcc gagtttgaac
      841 aggtaatagc gttcgcccaa tttgaccgga ttgcgggtaa cgtcgccacg gttcataccg
      901 gcaaactgcg aagccagcgg ctcgggaagc tgctgcgcca taatgaaacc gtcgaacgcc
      961 tgctcgtcgt tcggataacg cttcatcagc ccttcaaaag acagcccttt gagcaggagc
     1021 tgctgcgcct gacgcgcctc ctcttcggtt gcgaagctga cctgctgcaa tttgatcatg
     1081 cggatttgcc gctcataaaa ctgacgcagt gcgctttcgg aaaccgtttc cgaacgttcc
     1141 agaaaacgga cgtactcctc ggcataaaaa gacgcttcgg cgattttaaa gcggttttgg
     1201 acatccttat ccttatccaa accttccttc aatgccctgt ttttcaaaac ttccaaagtt
     1261 tgcagccggc ggacggcatc gtttcggatt gcctgcccgt ccggtctttg ggactgctcc
     1321 gcatgccggt ctgcctgctg catgatctgc gccaccagcg tatccaccaa agccgggtcg
     1381 atttcgggtg ctttggctgc cgcaaaacct gccaacattg cagcaataac tgcggcagcg
     1441 gtctttttct gtttcatggt attccctgtc ctgcgcggcg gcaaaacgcc tgtatgaacg
     1501 tcttgccgcc gtatcccgtt ttcagattat tttgcaggtt tgatgtttgc cttacccaac
     1561 agcgcaccga cggcacggtc aatccgttcc gcctgaaggt tgccggcaat ctgtcctttc
     1621 atttcgtcaa aagaaggcac tttcacctcg cggctgtcgt tgacataata aacgccgtag
     1681 aaatcgccgt ttttcagcgg cgttgccgta aattcgcctt ttttcaagtc cttaattgcc
     1741 tgataaagcg gcggaacacc ctgttccaaa tctttcagcg gcacatatcc gtccggcgcg
     1801 ccggtccgtt tggtgcggtc gttgagcgag tattgtttca aaacggcatc aaaacctttt
     1861 ttcgccttca aatcggcaac cgcttttttc gcattttctt ccttgtcggt caggatttcg
     1921 cccaactgga cttcctgcgt gcctttataa aaaccgctga tattgtcgta aacggctttt
     1981 acttcctgct cggaaaccgg ttgggttttg gcgatatgca gtgcgtatgc ctcgccgttc
     2041 aagccatatt ttaccgcctg ccaaacggtt ttgaaggacg gtttcttgtc gtcgcccgac
     2101 tttttcgctt cggcacgcaa tttggcaagc gcatctttaa actctgccga ccggtcgagt
     2161 ttcaggcgtt tcacttcctg tgcgaccacg gtgttgacca cttcgttttc cagcagggat
     2221 tggcgcagtt gcggcgtgtc ttcggcacgg ctgttttccg cacggaatgc ggcaacctgc
     2281 gcatcgatga cggaactgtc gattttctga ccgttaacgg ttgccagcgt ttgggcaaac
     2341 aggctgccgg aacaggcaag cagcgcaacg gaagtcagga tttttgcttt cataatcttc
     2401 tctctttaaa ttttaaattg aaatattcag gcagacttca ttcagtccgc cgtatggaaa
     2461 tactcgtcag gggtagccgc ccggatgccg agcgcgtgaa tcatgccgcc tgaaaataaa
     2521 tctttgagca gcgatttgac cgttttctga cggttcagac ggcttaaacc ttcaaaacgg
     2581 ccgctgacga ccaaaacggc ataatgtccg ccgccggtat tgcccgcgtg tcctttgtgc
     2641 agatggctct catcgccgat ttccaacacc agaggatcga gcgtctgcag gcgttcgcgg
     2701 atcaaatcga cagccggcat tatttgaaca ccgctttaaa cggcttgatc agcacttcgc
     2761 tgtacacgcc ggcatgaaca tagggatcgt cttcagccca agcctgcgcc gcatccaaag
     2821 actcgaactg tgccacaatc aagctgcccg aaacacgttc gggattgtcc ggcagcaggt
     2881 ttgggcctgc cgtcagcaga cggccttccg atttcagcgt ttccagccgt ttaaagtgtt
     2941 cgggacgtgc cgccatacgc gcttcatgca catcctcccc gtctgttgcc agcaacataa
     3001 aatattccac agtcaatcct cctttttcag ataggtactc agataaatac cctgaataat
     3061 aaagaaaaaa agcatcagcg cagtcgaacc gaacatctta tagttaaccc attgcgcttc
     3121 aaacctagta aacacaaacc agttggcaat acccataaaa atcagaaaac cgacccacat
     3181 atatgtcaat tttccccata cggcatccgg aagctgaatc tccctgccga tactcgcttt
     3241 caagccgttt ttacccgcaa ggtggctgcc cagcaggaat aacgccccgc accagaacaa
     3301 tactgtcggc ttccacataa tgaagcggct gtcgcccaaa acaatggttg cgccgccgaa
     3361 tacgacaatc agcaccagtc cgacccactg catcgtatcc agccttttat gcttccaata
     3421 caggaaagcc gcctgaacca cgcctgcaac caaggcaacc gccgcagcgg caatcatatt
     3481 tttggtaacg gtataagtag caaaaaacaa gatgacggac aaaaggtcgc tgacaaattt
     3541 catatccaaa tgagaaagca aatcaaataa acgctaatca tacaggcaga accctatctt
     3601 tgcacctgca aaaccaacaa attttgtttt gattaatgca aatatgcaca gatgtttttg
     3661 aaaaaagatg gaaatatgtc ataatttata aaattggctt taaaatacag cttcggtcgg
     3721 aacctctgcc tatccgcacc ctatcgtccg tattgcctat gcctgatgac gaaatttaca
     3781 gactgtacgc ggtcaaaccg tattcagccg ccaacccaca ggggatacat cttgcaaaaa
     3841 caacagacaa atcaaactga ttgccgcctc catcgcagtt gccgcatcct ttcaggcaca
     3901 tgcgcgactg ggcggactga atatccggtc caaccttgac gaaccctttt ccggcagcat
     3961 taccgtaacc ggcgaagaag ccaaagccct gctaggcggc ggcagcgtta ccgtttccga
     4021 aaaaggcctg accgccaaag tccacaagtt ggacgataaa gccgtcattg ccgtttcttc
     4081 cgcacaggca gtccgcgatc ccgtcttggt gttccgcatc ggtgcaggcg cgcaggtacg
     4141 cgaatacacc gccatcctcg atcctgtcgg ctgctcgccc aaaaccaaat ctgcactttc
     4201 agacggcaag acacaccgca aaaccgctcc gaaagcagag tcccaagaaa atcaaaacgc
     4261 caaagccctc cgcaaaaccg ataaaaaaga cagcgcaaac tcagccgtca aaccggcgca
     4321 taacggcaaa acccataccg tccgcaaagg cgaaacgctc aaacagattg ccgccgccat
     4381 ccgtccgaaa cacctgacgc tcgaacaggt tgccgatgcg ctgctgaagg caaacccaaa
     4441 tgtttccgca cacggcagac tgcgtgcggg cagcgtgctt cacattccga atctgaacag
     4501 gatcaaagcg gcagcaccca aaaaaatcaa agcggaacaa cccaaaccgc aaacggcgaa
     4561 acccaaagcc gaaactgcat ccatgccgtc tgaaccgtcc aaacaggcaa cggtagagaa
     4621 accgatagag aaacctgtcg aaaaaccggt tgaaaaacct gaagcaaaag ttgccgcgcc
     4681 cgaagcaaaa gcggaaaaac cggccgttcg acccgaaccc aaacccgcag tgtccgaaac
     4741 tccggcttcg gcgacagaac gccaacccgg acctgtaccc gctgcaaata ctgccgcatc
     4801 ggaaaccgct gccgaatccg cccccccaag aagccgccgc ttctgccatc gacacgccga
     4861 ccgacgaaac cggtaacacc gtttccgaac ctgttgcgga aacagccagt gaagttgccg
     4921 ccgaacccgt tgccgaacct gtcgaacagg tttctgccga agaagaaacc gaaagcggac
     4981 tgtttgacgg tctgttcggc ggttcgtaca ccttgctgct tgccggcgga ggcacggcat
     5041 tgatcgccct gctgctgctt ttacgccttg cccaatccaa acgcgcgcgc cgtaccgaag
     5101 aatccgtccc tgaagaagag cctgaccttg acgacgcggc agacgacggc ataaaaatca
     5161 cctttgccga agtcgaaact ccggcaacgc ccgaacccgc tccgaaaaac gatgtaaacg
     5221 acacacttgc cttaggtggg gaatctgaag aagagttatc ggcaaaacaa acgttcgatg
     5281 tcgaaaccga tacgccttcc aaccgcatcg acttggattt cgacagcctg gcagccgcgc
     5341 aaaacggcat tttgtccggc gcacttacgc aggatgaaga aacccaaaaa cgcgcggatg
     5401 ccgattggaa cgccatcgaa tccacagaca gcgtgtacga gcctgagacc ttcaacccgt
     5461 acaaccctgt cgaaatcgtc atcgacacgc ccgaaccgga atctgtcgcc caaactgccg
     5521 aaaacaaacc ggaaaccgtc gataccgatt tctacaacaa cctgttctca aacaaccata
     5581 tcggcacaga agaaacagct tccgcaaaac ctgccgcacc ctccggactg gcaggcttcc
     5641 tgaaggcttc ctcgcccgaa accatcttgg aaaaaacagt tgccgaagtc caaacaccgg
     5701 aagagttgca cgatttcctg aaagtgtacg aaaccggtgc cgtcgcggaa actgcgcctg
     5761 aaacgcccga tttcaacgcc gccgcagacg atttgtccgc attgcttcaa cctgccgaag
     5821 caccggccgt tgaggaaaat gcagcggaaa tcactttgga aacgcctgat tccaacacct
     5881 ctgaggcaga cgctttgccc gacttcctga aagacggcga ggaggaaacg gtagattgga
     5941 gcatctacct ctcggaagaa aatatcccaa ataatgcaga taccggtttc ccttcggaat
     6001 ctgtaggttc tgacgcgcct tccgaagcga aatacgacct tgccgaaatg tatctcgaaa
     6061 tcggcgaccg cgatgccgct gccgagacag tgcagaaatt gctggaagaa gcggaaggcg
     6121 acgtactcaa acgtgcccaa gcattggcgc aggaattggg tatttgattc ccaactgccc
     6181 tttcgcagat caaggatgcc gtttcagacg gcatcttttt ttgccttatc ggtgtaacgg
     6241 ataaagtttg aaccggcaca ggctcaaaca gcaggccgtc ggcaacaaaa tgccgtccga
     6301 aacccctaaa ggcttcagac ggcattggcg gcggcggtat caggtattcg cataggcaac
     6361 cgaatcgtcg ccgcttttct tttgaacgaa ctcgattttg catccgtcgg ggtcttctac
     6421 gaaggctatc acggttgtgc cgtgtttcat caggccggct tcgcggacga cgtttccgcc
     6481 ctgccgcttc acgcgttcgc aggcttcgta ggcatcgtcc acttcaaccg cgatgtgtcc
     6541 gtaggcgtcg cccaagtcgt atcgttccgt atcccagttg tgcgtcagtt ccaaaaccgt
     6601 gctgtcggtt tcgtcgccgt aaccgacgaa ggcaagggta aatctgcctt cgggatagtc
     6661 ttttcggcgg agcagtttca tacccaaaac gttttggtag gaatcgaggg atttttcgag
     6721 attgcccacg cggagctata gtatggagta agcgcatttt tcgtgttcct ctcggtggtg
     6781 gttaaacttc gattttattc ggcgtaaacg tctgccattt gttgcaggcg ggacagtgcc
     6841 agaaaaagac ttgggatttg aagtggcagt tgcggcaacg gtacatcacg ctgcgctgga
     6901 gctgccgtcc gataaccgaa cgcatcatgt cggcatcggc tttccaagcc ggatccaaat
     6961 cgctgagttt caaaccgagc aggcggtaca cgccgttaag gtcgggcttg cggcggacaa
     7021 gctcgacggc ggtttgcgcg gcttctttct cgcccttaag cagcagggat ttctcgtaca
     7081 cgacattgat caggtcaagt tcgggaaacg tctgcatata tcctgtcaga cggttcaagc
     7141 cttcttcagg ttttccctgc gcggcatagg cttcgtaaag cttctcgccg accatgctca
     7201 agtatgcatg gttttgctgc tcgatggcgg cataggcttc gacggcggca gggaaattgc
     7261 cttgtcggtg ttcaatgtcg cccaaaatca tgttggcgcg ggtgcatttt ttgttggctt
     7321 cgagtgcctt gccgacattg aaacgcgcgg catcgaaatt ggacttgaac agcgcggctt
     7381 gggcaagttc gcaataaaac tgtgcaatct caaactgata tgtctgttcg tcgtgactaa
     7441 gaagttgggc ggtttcaacc gctttttccc aatccctgtc ctgctggtag atattgagca
     7501 ggtgctgtct ggcttcacgc gccatttcac cgtcttgcag ccccaaaaaa atctgttcgg
     7561 cacgatcgac caaacccgca ctttggtagt tttgcgccaa ttcaaacagg acgcgcgcgc
     7621 gcttttcgcc gaccgtatcg ggagaatcga gcattgtccg gtgtatgttg atggctttgt
     7681 cgttttcgcc acgctgacgg taaagtttgc cgagggtgag gttcaaatca tacgattgcg
     7741 gccggccgtc gacgacttcc gccaactccc ttgccgcgcg cccgctgttg cggtcgacca
     7801 aagcgtccag gcttttataa aatcccgaag ggatgctttt tgcctgcttc aatacggttt
     7861 tcatatccac gcgggcggca aaccagccca tcgtgaagaa gacgggcaaa aggataatcg
     7921 gcagcaggat aatccacaat tcgttgtcca tatcggcttt cttaaggctg tttggcagat
     7981 tcggcagcat tttgtatcgg cggtgcagtc aatttctgtc cgctcaagcg cgcacttttc
     8041 ttcacttccg cacgcaggcg gctgttttcg ccgcgcaagg acagcagccg cccgaacagg
     8101 gcaaacattc cgaacacgat gccgacgaca aacgcgccga acaatacgac aatcagcggc
     8161 agattggcac tctgccccgg aagataggaa aaggtaacgg catccatatt aatgacggca
     8221 agcagcagga agagcagcag gataatgatt ttgatgacgg tatagataag tttcatgttt
     8281 gctccgtaaa taaaaacgcc gcacggacag cttcgcactc tgaaccaaga ttgcgttagt
     8341 ttaaacgatt tgcacggttt cggatagaat gcggcagcgt gttcggacgg catacggagt
     8401 atgcgattgc cgacaatttt accaaataca ccgctccttt ccatttgaaa aataacggat
     8461 tggacaccgc atcgacagaa aaacccgccg cgcacttgtc aaaaccctgt ttgcaggcgt
     8521 atctttacaa ccttcaaatt caaaccgttc attgaaacat atcagaataa gaaaggcttt
     8581 acatcatgag cagacccgta cccgccgtat tcggcagcgt ttttcacagt caaatgcccg
     8641 tcctcgccta ccgcgaaggc aaatggcagc cgaccgaatg gcaatcttcc caagacctta
     8701 cccttgcacc cggcgcgcat gccctgcatt acggcagcga atgctttgaa ggtttgaagg
     8761 ccttccgtca ggcagacggt aaaatcgtgc tgttccgtcc gaccgccaat atcgcgcgta
     8821 tgcggcaaag tgcggacatt ttgcacctgc cgcgccccga aacccaagct tatctcgatg
     8881 ctttggtcga actggtcaaa cgtgccgccg acgaaattcc cgatgcgcct gccgcgctgt
     8941 acctgcgccc gaccttaatc ggtaccgatc ccgttatcgg caaggccggt tctccttccg
     9001 aaaccgccct gctgtatatt ttggcttccc ccgtcggcga ctatttcaaa gtcggctcgc
     9061 ccgtgaaaat cttggtggaa accgaacaca tccgctgcgc cccgcatatg ggccgcgtca
     9121 aatgcggcgg caactacgct tccgccatgc actggttgct gaaggcgaaa gccgaatacg
     9181 gcgcaaatca agtcctgttc tgcccgaacg gcgacgttca ggaaaccggc gcatccaact
     9241 ttatcctgat taacggcgat gaaatcatta ccaaaccgtt gacagacgaa ttcctgcacg
     9301 gcgttacccg cgattccgtg ctgactgtcg ccaaagattt gggctatacc gtcagcgaac
     9361 gtaatttcac ggttgacgaa ctgaaagctg ctgtggaaaa cggtgcggaa gcgattttga
     9421 ccggtacggc cgccgtcatc tcgcccgtta cttccttcgt catcggcggc aaagaaatcg
     9481 aagtgaaaaa ccaagaacgc ggctatgcca tccgtaaggc gattaccgac atccagtacg
     9541 gtttggcgga agacaaatac ggctggctgg tcgaagtgtg ctgatgcttt aaataaaaaa
     9601 aatgccgtct gaaacccgtt tggcgtttcg gacggcattt tcgcgttcgg accgtttccg
     9661 ccgcacctgc agcaagtcag tacaaaggca atcggttaaa acaagcgtcc gcatttccca
     9721 tcccgcctgc cgcaagtcgg gcatttccct ggaaatacgc ttcaaacggc aaaccgccgc
     9781 ccgaaaccga tactcggcac ggacggcgca cggatttgaa aacggcggat tatccctcgg
     9841 tgctcaaggc attgatgctg taaccgccgt caacataagt gatttcgccg gtaatgccgg
     9901 acgacaggtc ggacagcagg aaggcggcag tattcccgac ttcttcgatg gtaacgttgc
     9961 ggcgcagcgg gttgtgggcg gcgacgtgtc ccaagagttt gccgaaatcg gcgatgccgg
    10021 aggcggcgag cgttttgatc gggccggcgg aaatgccgtt gcagcggatg ccctctttac
    10081 ccagacaggc ggcggtaaag cggatgcctg cctcaaggct ggcttttgcc atacccatca
    10141 cgttgtaatt cggaatcgcg cgtaccgcgc ccaagtagct aagggcgacg atggcggagt
    10201 ttctgccgcg catcatcgga cgggcggctt ttgccaatgc gggcaggctg tatgcggaaa
    10261 tttcgtgtgc ggtgttgaac gcttcgcggc tgatgctgtc gaggaagtcg ccgctcaagg
    10321 cttctttcgg cgcgaagccg atggaatgca ccaaaccgtc caagccatcc caatgtttgc
    10381 ctaagtcggc gaacacttgg ttgatttcgt cgtcgctggc gacatcgcag cggaatacga
    10441 gttcggaatc taattccgcc gccattttgc ggacgcgctc ttccagtttg tccacaacgt
    10501 aggtaaacgc cagttccgcg ccctgttcgc ggcaggcttt ggcgatgccg taagcgatgg
    10561 aacgctcgga aatcatgccg gtaatcagaa tttttttgcc ttgcagaaaa cccattttgt
    10621 atccttcaaa tagtgttcgg ttcgttctaa accgttgatt atagcaaatt gtccctgttt
    10681 ctgtgttttc acgttgcagc gtgcagacgg caatgccgtc cgaagcggat ttcagacggc
    10741 attggacgtt tcaaatacgg tttaaggcat cagatgccgc gcaagagttc gttcacgctg
    10801 gttttggcgc gggtttgcgc gtccacgcgt ttgacgatga cggcacagta aaggctgtgg
    10861 ctgccgtctt tggaaggcag gctgccggac acgacgaccg aacctgccgg tacgcggcct
    10921 tggtagattt cgcctgtggt gcggtcgaag attttggtgg attgaccgat gaacacgccc
    10981 atcgaaatca cgctgccttc ttcgacaatc gcgccttcga cgatttcgga acgcgcgccg
    11041 atgaagcagt tgtcttcaat gatggtgggt gcggcctgca ggggctcgag tacgccgccg
    11101 atgccgacac cgccgctcaa gtggacgttt ttgccgattt gcgcgcaaga gccgacggtc
    11161 gcccaagtat cgaccatcgc gccttcgtcg acgtatgcgc cgatgttgac gtaagacggc
    11221 atcagcacgg cgtttttggc aacaaagctg ccgcgtcggg caaccgcacc cggaactgcg
    11281 cggaagcctg cgtttttgaa ctcgtcttca gaccagtcgg caaatttggt cggcactttg
    11341 tcgaagtatt tgttcacgcc gtcgttgagg acttcgttgt cttggatgcg gaaggacagc
    11401 aacacggctt ttttcgccca ttcgttgact ttccactcgc ccacgcccaa gcgttcggcg
    11461 acgcgcagtt tgcctgaatc gagttggcgg atggtttcca acacggcttc tttgacttcg
    11521 ggggcaacgg tggtcggagt gatgtccgcg cggttttcaa aggcggtttc gataatgttt
    11581 tgcaaagaca taatgtttcc ttggttcgag atatttcggg acgcttgccg gtattgttca
    11641 gacggcatgg cggttggaac aaacgtgcta ttgtgccata caaacgcccg cccgtcagcc
    11701 gcgccgcagg cttgccggat gccggcgttt cggaatcggg cgggcggcag gcatatgtct
    11761 ttttccgcgc ccgaatcaat acagaaaggc ggcggtactt ttatgccgcg ccgccgcctt
    11821 tcagacggca ttcgcggtaa aacgccatca gcccttcggt cgaggcatcg tgtacggacg
    11881 tgccgccttc caattcgccg atgatggttt ttgccaactg tttgccgtac tcaacccccc
    11941 actgatcgaa ggggttgacg ttccatatcg cgccttggac gaaggttttg tgttcgtaag
    12001 ccgccatcag catacccaga ttgtagggcg tgaggcggtc gagcaggatg ctgttgctgg
    12061 ggcggttgcc ggggaactct ttgtgcggcg cgaggcgttc gcgttccgct tcgggcaaat
    12121 ctgccagttc ggcgcgtgct tcgtctaagg ttttaccctt catcagggct tccgcttggg
    12181 caaaggcgtt ggcaacggta aaacggctgc gtccgtcctc cgcgccctgc gccgtcatcg
    12241 ggacgataaa gtcgcaggga atcaggcgcg tgccttggtg gagcagttgg aaataggcgt
    12301 gctggcagtt gaccccttca ccgccgaaca cgatgccgcc cgttttgcac gcggcgggac
    12361 tgccgtctga agcgcggctt ttgcccaaac tctccatatc gagctggttc agccacgccg
    12421 gcagcaggcg caggttgtgg ctgtacggaa cggcggtctg cccgtccgcg tgctggaaat
    12481 tgttatacca cacggcaatc agtgccatca gaacgggaat gttgcgacgc ggcggcgtgt
    12541 ggaaaaaatg gctgtccatc gcgtgcgccc ccgctagcag ttcgcggaaa cgcgccccgc
    12601 caaccgcaac catcacgggc aaaccgacgg gcgaccagac ggaatagcgt ccgcccaccc
    12661 agtcgtacat cgcaaacacg cgttccgccg caatgccgaa actttgcgcc gcttccgtat
    12721 cggcagacac cgcgcaaaaa tggcgcgccg tttcggattc cgagaacccc gcaccgcgat
    12781 accacgcctt gaccgcctct gcattgagca gggtttccgg tgttttaaag gacttgctgg
    12841 caacgcaaaa cattgttgtt tcggggttca gacggcataa gacctcatcc aggcaggcag
    12901 ggtcggcatt agaaacaaaa tggacggaaa tctgccgtct gaacggctcg agcgcctgca
    12961 cgcacattgc cggcccgagg tcggatccgc ctatgccgat gtggacaaaa tccgcaatcc
    13021 gttttccggt tatcccctga tataaaccgt cgtccaaact gtgtgcaaac ttcaacgcac
    13081 gatttaactc gcggcggatt tcgggcaaca cgtccctgcc gtccgcataa acggcatccg
    13141 caccgtcggg caggcgcaaa gccgtatgca gcgcggcacg cccctcgctg ccgttgactt
    13201 tcgcacccgt ccgcaaagca cgcattttcc cttccaaatc cgccgtctcc gcaagacggc
    13261 agagcagttg cagcgtatct tcgccaaaac ggtttttgct gtaatcgaac aacatcccgt
    13321 ccaaacgctc atgcatacgc tcaaaacggt cgggttcggc agcaaagcgg tcgcgcaaaa
    13381 ggatgtggca cgtatcctga taatggcgtt cgagcgcata ccatgcacgg gtaaaggcat
    13441 ccatctgttt tccttgattt ttcagaaccg gattaaaata tagcagaatg tagtctaaca
    13501 aacggcagac actttagcga atctcaggaa caccgcccgc cgcgtccaat aacaccttat
    13561 ttccatatgg cgaaaatata gtgaattaac aaaaatcagg acaaggcggc gggccgcagg
    13621 cagtacggat agtacggaac cggttcgctt ggtgcttcat taccttaggg aatcgtcctc
    13681 tttgagccgg ggcgggccaa cgccgtaccg gtttttgtta attcactata ccctccggcc
    13741 ggcatttgac ccgcaacaat atcctgcaag atttattgtg tgcgcataaa tgccggacag
    13801 ccgcctaaat taaggtataa tcgcatccga ttctgtcccg tttgccgccc gcaggcggga
    13861 tgtcggcggt tttcatctta ccgggaacac gccgatactg ttttttaacg atgtttctta
    13921 cattttattc caattacttt acggggctag aatatggcta aaaatggggg attttctttg
    13981 ttcgcaaaga aagaaaaacg ctttatcttt gaaggcaggc attctgcctc cgacaaactg
    14041 gtcaacggcg aagtatccgc gtttaccgaa gaagaggcac gcaaaaaact ggcaaaacgc
    14101 ggcatccgcc cgttgcagat tacccgtgtg aaaacaagct ccaagcgcaa aatcacacaa
    14161 gaagacatta ccgttttcac ccgccagctt tccacgatga ttaaagcggg cctgccgctg
    14221 atgcaggcat ttgaaatcgt ggcgcgcgga cacggcaacc cgtctatgac ggaaatgctg
    14281 atggaaatcc gaggccaagt ggaacagggc agctcgttga gccgcgcatt ctcaaaccac
    14341 ccgaaatatt tcgaccgctt ctactgcaac ctggttgcgg cgggcgaaac gggcggtgta
    14401 ttggaaagcc tgctggacaa attggcaatt tacaaggaaa aaacccaggc catccgcaaa
    14461 aaggtaaaaa ccgcgctaac ctatccggta tccgtcatcg ccgtcgccat cggtttggta
    14521 ttcgtgatga tgattttcgt actgcccgcc tttaaagaag tttacgccaa tatgggcgcg
    14581 gagcttcccc cactgaccca aacagtgatg gatatgtccg actttttcgt ctcatacggc
    14641 tggatggtgc tgatcgcact gggctttgcc atatacggct tccttaaatt gaaagcgcgt
    14701 tcgattaaaa tccaacggcg tatggatgcc atactgctgc gtatgccgat tttcggagac
    14761 attgtccgca aagggacgat tgcccgctgg ggcaggacga cggcaacgct gtttgccgcc
    14821 ggcgtgcctt tggtcgatgt attggactcc actgccggcg cggcgggcaa tttaatctat
    14881 gaagaagcca cccgggaaat ccgtacgcgc gtcatccaag gtctgtctat gacttcgggg
    14941 atgcgtgcga cggaactgtt ccccaatatg atgttgcaga tgtcctccat cggcgaggaa
    15001 tcgggttctt tggacgatat gctcaacaaa gccgccgaat tttacgaaga cgaggtggac
    15061 aatgcggtcg gcaggctgtc cgctatgatg gagccgatta ttattgtgat tttgggcttg
    15121 gtcatcggta cgcttctggt cgctatgtat ctgccgctgt tcaacttggg caacgtggtc
    15181 gcctgatttg ccgcacagat ccggcgcgga ttggttctgc gccggtttgt ttttgctttg
    15241 aatatatcaa ggacaaaata tgtctgattt gtctgtattg tcgccgtttg ccgtgccttt
    15301 ggcagcagtt ttggggctgc tggtcggcag cttcctgaat gtcgtcattt accgcgtacc
    15361 cgttatgatg gaacgcggct ggacggtatt tgccaaagaa catttaaacc tgccgctgac
    15421 cgacgatgaa agccgtacct tcaacctgat gaagccggat tcctgctgtc ccaaatgccg
    15481 tgtgccgata cgcgcgtggc agaacatccc gattgtcagt tacctgctcc tgcgcggcaa
    15541 atgcgcttcc tgccaaacca aaatcagcat acgttatccc ttaatcgagc tgctgaccgg
    15601 cgtattgttc gggctggtcg cctggcaata cggctggtct tggattacgc tgggcggttt
    15661 gatactgacc gcgtttctga tttccctgac ctttatcgat gcggacaccc aatacctgcc
    15721 cgactcgatg acattaccct tgatctggct ggggctgata tttaatttgg acggcggctt
    15781 cgtgcctttg cagtctgccg ttttaggtgc ggttgccggc tatagttcat tatggctctt
    15841 atgtgcagtg tataaactgc tcacaggaaa aaccggtatg ggcaacggag atttcaaact
    15901 gattgccgca ttgggcgcgt ggctcggcat atccgcattg cccgtgctga tttttgtttc
    15961 ctctctgatc ggtttggtcg cggcaatcgt tatgcgcgtc gccaaggggc ggcattttgc
    16021 cttcggcccc gcactgacag tttcgggctg gataattttt acggcaaacg attccgtatg
    16081 gcgggcggtc aactggtggc tgacccatcc ggtgagatga cagcatgggt cggactgacc
    16141 ggcggaatcg gcagcggcaa atcggcagcc gcgcaatatt ttgccgattt gggcgtgccg
    16201 cgcatcgatg cggacgcggc ggcgcactcg ctgacggctt cagacggcat cgccctgccg
    16261 gaaatcaggc ggctgttcgg cgacaccgtt ttcgacacac agggtttgtt gcggcgcgac
    16321 atattgcgta aagaaatctt tgcctcccca tcgcgcaaag ccttgctcga atccgtgatg
    16381 ttgccgctga ttctctcaga aatcaaaaaa cagcaagaaa cctttaccga tgcggtttac
    16441 ggcattgtcg aaattccgct gctgacggag aagcgtcaat ttatcagcct gatacggcgt
    16501 gtcctgacca taagcgcacc tttggaaaaa cgtatcggca gggtaatggc acgcagcggg
    16561 ctgacgcgcg gcgaggtggc ggacatcatc agccatcagg catccgaatc cgaacgcctg
    16621 ctgcttgcag acgatgtact gctcaatgac ggcagcctca aaagcctacg tgagaaaaca
    16681 atgctcctgc acgcgtttta ttcagggatt ttcgcctcaa aaccaacaca aggaaaacac
    16741 aatggctgaa tcgcggcaaa cacgccttca agtcaaatgt ccgacctgtc aaacggcagt
    16801 agtatggaaa ccggaaaacg cattccgccc gttctgttcg caacgctgca aactgatcga
    16861 cttgggcgga tgggcagacg ggaaatatac ggtttccggc caaacggaaa gtttgccgga
    16921 aatatccgaa cccgacgggg cataccgctg accgccccgc cttcccggca aacaccctga
    16981 aaggtcaaat gccgtctgaa acaaacacgc ttcagacggc attttcattc tcaaacctaa
    17041 tcgttggtat ttgccgttac ctcttccaat gaagtaatgc cctgcataat tttcaaaata
    17101 ccggcccggc gcaaatccac cataccctcc ttataggcaa cgtccaaaat acccacttcc
    17161 gtaccgttgt tcataatcac acgctgcatt tcttcgctga tgggcataac ctcatacacg
    17221 cccgcacgcc ccttataacc ctgcccccgg caacggtcgc aaccgacggc gcggtaaagt
    17281 ttccaatctt ttgcaagatc ctcatcggtg aaaccgactt ccttcaaagc agaggcagac
    17341 gggcgttcca cttcctgttt gcagctcgaa cacagcctgc gtaaaagacg ctgcgccata
    17401 atcaggctga ccgaactggc aatattaaac ggcgcgacac ccatattcag catacgcgac
    17461 aacgtcgccg gcgcattatt cgtgtgcagt gtggaaaaca ccatatgccc tgtttgtgcc
    17521 gccttaatcg caatatcggc agtttccaaa tcacgaatct caccgaccat aatgatgtcc
    17581 gggtcctgac gcaggaaaga cttcaaagca gcggcaaaag tcagaccctg cttatcattg
    17641 acgttaacct gattgatgcc cggcaggtta atctcggcag ggtcttccgc cgttgcaata
    17701 tttaccgact ccgtattcaa aatattcaaa caggtataga gcgacaccgt cttacccgaa
    17761 cccgtcgggc cggttaccag caccatcccg taaggacggt gaatcgcttc caacaacaat
    17821 tttttctgga acggctcaaa accgagctgg tcgatgttca aagacgcggc atcggaattc
    17881 aaaatccgca tcacgacctt ttcgccaaac agcgtcggca atgtgctgac acggaaatcg
    17941 acaggcttgc cgcccttttg aaaggtcagc tgcatcctac cgtcctgcgg tatccgtttt
    18001 tcggaaatgt ccaaacgcga cattacctta atccgggaag caagctgccc ccttaccgca
    18061 atgggcggct gaaccacctc gcggagctgc ccgtccacac ggaaacggat acgcgcattg
    18121 tgttcgtaaa actcgaaatg gatgtcggat gccccgctac gcaaggcatc cgacaaagtt
    18181 ttatggataa acctcggaac agggccgtct tctgcctcct cgttgtcgat atacagggtg
    18241 tggctttcct cttcctcttg cccctcccca agctcctgaa gcagcgatgt cgaacgcgaa
    18301 cccacccaat cgagcaaacc cgccaactgg tcatcctcga caatgaccaa ctcaaccgca
    18361 atccctgcgg cagaaacggt tttctgaatt tgcggcatct gggtcggatc ggaaaccgca
    18421 aaaaatactt tgtcgccccg acggaaaacc ggcacacagt ggaactccac catctgctcc
    18481 tccgtcaaca cccccatcag caccctgtgg cgcggataat gacgcaaatc aagaatcgaa
    18541 taactgaaca ccctcgcaat caatgccgca agcgacttgg gcgaaatgac accgtctgaa
    18601 aacagcatcg gcaacacttc cttacccgcc tgcgactcat tgtagtaatg ctcggcctgc
    18661 tcaacagtaa ccacctggtt ttgaaccaga atcctcagca aacctacgct catacgacct
    18721 tatccccaaa tttattcatt gttatacctg tacagatttt atcataaaac aacagatttc
    18781 caagcctgaa catcttttac gaagcctgaa aatcatttca cgattctgcc gtcctaaagg
    18841 tcggtttttc aagcaggaag aaaaagtttt cagatggcaa aaaagccctc cagcactgaa
    18901 aggccttata tcggaaactt cccgcaacac gggaaacaga caaatgcaaa ccgtcaaacc
    18961 tcgccaacag gaatcgaacc tgtattttac gcttaggagg catacgttct atccgttgaa
    19021 ctatggcgag ccgaaatgaa aagggagatt ttaaaccttt ccggcaacaa aagacaagat
    19081 gtcggccgca atcagcggcc cgacagggat accgcccata aatgccaccc cgatgaccgt
    19141 accgatcagc aagcccgtta ccaataccgg ctgccgtccc atcaaaggca cgccgcatcc
    19201 cgccagccaa gccacgaaaa taccgataaa aacggcggat atcattttaa aattcaaaaa
    19261 ttcggcaaca ggaggaacct gcgcctttcc tgaaaccaac ggactcaaaa cccctatggt
    19321 caaaagaatg ataccgaggt tcaacccgtg cttctcgacc aacgggacaa actgtaccaa
    19381 tgccgtctgc tgcatcagca gcaatatggt tgccgagacg gtaatcgaat tgttgttgct
    19441 gaccaccccc aacagaatca gcgtaaccag aaacagggga acaaaactga aattcatcgg
    19501 ttacgacacc ctgaccaaag attccttcgc cagctgcatc atagcatcct tcacttcgct
    19561 gtcggacagg gcatccaacg cggcaacggc acaatcgact gctttgcgcg cctcgcctat
    19621 cgaatatgcc aaagcatccg aacggacgac ataatcgcgg attttttcaa aatagccgcg
    19681 atctgcattt tccaaagcag tacgcacatc gttcgcaacc tgttcggaac cctgacgcat
    19741 cagataaatc aaaggcaggg tcggttttcc ttccgccaaa tcgtcgccga cgtttttacc
    19801 tgtttcttcg gtttcccccg aataatccag cacatcgtca ataatctgga atgccgtacc
    19861 gacgtacata ccgtagtctt tcaaggcctg ttcgtgcccg ggggaagctt tgcccaaaat
    19921 tgcgccgact tgagcggcag cttcaaacaa ttttgccgtt ttatattgga tgacttggat
    19981 atattgttct tcggtaatgt ccgtattgcc gatgttcatc agctgcatga cctctccctc
    20041 ggcaatgatg ttggtcgcat ccgccatcac ttccaaaacg cgcatactgc ccgaggcaac
    20101 catcagttga aatgcacggg tatataaaaa gtcgccaacc aacactgccg ccgcattgcc
    20161 gaacagattg tttgccgttg cccgtccacg gcgcaaatcg ctttcatcga cgacatcgtc
    20221 gtgcaggagt gtggaagtgt ggataaactc gaccatcgct gccagcgaat acagtttctc
    20281 gtcatcataa ccgaccgcct tacccgccaa aatcgtcata atcggacgca ggcgtttgcc
    20341 gcccgcgctg atgatatatg taccgatttg cgaaatcagt gcgacatcgg attgcaccgc
    20401 acggttgatg acttcattga ctttggcaag gtcttcaggc agatgtcgct ggaaataggg
    20461 cagattctcg agcatggcag actctcaggt ttacgtttcc gtcaaacggc agataccgct
    20521 tgaaagctga ttcaaatagc gggcaattat atcagcaaag ccatcagggt acatcttttc
    20581 agacggcata tgattgcccg aaccgcgcaa actttgacaa aacaggcaaa taacaataca
    20641 atgcccaatt tcgcacaatg gtgtgcggat attaaccata acccttatgg agttgagtat
    20701 gtacgcggtc gtaaaaaccg gcggcaaaca gtataaagtt tccgtcggcg aaaaattgaa
    20761 agtagaacag ataccagccc aactcgacag ccaaatcgaa ctgaccgaag ttttgatgat
    20821 tgctgacggc gaatctgtaa aagttggcgc accctttatc gaaggtgcaa aagtaacggc
    20881 taaagtagtg gcacacggtc gtggcgaaaa agtccgcatc ttcaaaatgc gccgccgcaa
    20941 acactaccaa aaacgccaag gccaccgcca aaatttcacc caaatcgaaa tcgtggcaat
    21001 cgcctaacct taaaacttag gagcattaca aatggcaagt aaaaaagcag gcggcagcac
    21061 ccgcaacggt cgcgattcag aagccaaacg cttgggcgtt aaagcctacg gcaacgagct
    21121 gattcccgca ggttccatca tcgtacgcca acgcggtacc aaattccacg caggcgacaa
    21181 cgtaggtatg ggcaaagacc acactttgtt cgccaaaatt gacggttatg tcgaattcaa
    21241 aaccaaaggc gcgctgaacc gtaaaactgt cagcatccgt ccttacaccg gttctgaaga
    21301 ataattcaac cgatacctga agccgcatct tttcacgatg cggcttttta cataccccat
    21361 acccgatttg atacacatta agctgaaagt aaaaatccgc atacaccctc ccctgcatat
    21421 ttcttcaaca acgggttttg atataatcgc ctatctgtta cagatagttc aaacggcatt
    21481 caaaccctta caaatgccgt ctgaaatcat cggcatccgc ctatgcaaag gatattttat
    21541 gagtttacac agcgacatcc tcgtcgtcgg cgcaggcccg gcgggtttaa gttttgcagc
    21601 cgaacttgcc ggcagcggtt tgaaagttac tctgatcgaa cgcagcccac tgactgttct
    21661 gcaaaatcct ccctatgacg gacgcgaaat cgccctgacc catttttccc gcgaaatcat
    21721 gcagcgtttg ggtatgtggg acaaaattcc cgaaaacgaa atctacccct tgcgcgatgc
    21781 caaagtgctg aacggacgtt ccgactacca gctccacttt ccccaaccga ctgaagcgcg
    21841 cggcgagcct gccgactgtt tgggctatct gatttccaac cacaatatcc gccgcgccgc
    21901 ctatgaagtc gtatcacaac tcgacaatgt cagcattctg accgatacgg ccgttaaaga
    21961 agtcaaaaca tctgataatg aagcgcaagt ctttttggaa aatggagaca ttctaaccgc
    22021 gcgtctcctt ttggcagcag acagccgttt ctcacaaacc cgccgacaac tcggtatttc
    22081 ttcagatatg cacgactaca gccgaaccat gttcgtctgc cgcatgaaac ataccctttc
    22141 caaccaacat accgcttacg aatgcttcca ttacggacgc accatcgcac tgctgccact
    22201 ggaaaagcgc ctgaccaata ccgtcattac agtcgatacc gataaaatca acagcgttca
    22261 aaacctttcg cccgaagaat tggcggcaag cgtaaaagaa cagctcaagg gcaggctcgg
    22321 cgacatggaa ctggtcagca gcatccacca ttaccccctt gtcggcatga ttgccaaacg
    22381 tttctacggc aaacgcagcg cgctgatcgg cgatgcggct gtcggtatgc accccgtaac
    22441 ggcacacggc ttcaacctcg ggctttccag cgcggatatt ctggcaaaac tgatactcga
    22501 agccgaacag cgcggtcaag acatcggcgc ggcttccttg ttggaaaaat acagcagcaa
    22561 acatatgctg cacgcccatc cgctttatca cggcaccaat atgatgctga aactgtttac
    22621 caacgaaacc gcgccggcaa aactgttgcg cggtttggta ttacgagccg gcaacaactt
    22681 cccgccgctg aaaaagctga ttaccaaaca actgaccggt tgaaaactgc cgaaatgccg
    22741 tctgaaacgt ttcagacggc atttttgtat tatgaaccgt tttgcggttt ccatatcctt
    22801 ggcagacggt ttgcagcaga ttcgcatacg gcagatgttt caagcagaaa ggaaacacaa
    22861 taaaaataaa aaagcctccg aacagtcgga ggctttgctt tcaaactgaa attatttcag
    22921 tttggtttct ttgtacacta cgtgtttgcg ggcaaccgga tcgaattttt tgatttccag
    22981 tttgccgggc atagtgcgtt tatttttggt agtggtgtag aagtggccag tacctgcacc
    23041 ggattccagt ttgattttat cgcgcattgc agtaatcctt aattaaatag tgtttaaatt
    23101 aagcttcgcc gcgagcacgc aaatcagcca atacgacatc aatgcctact ttgtcgatgg
    23161 tacgcaatgc agcgttggaa acgcgcaggc gaacccagcg gttttcactt tctacccaaa
    23221 aacgacgtga ttgcaagttg ggcaaaaaac ggcgtttggt tttgttgttg gcgtgcgata
    23281 cgttgttgcc ggacatcggg cgtttaccgg tcactttgca aactcgtgcc ataatcagtc
    23341 tccaaacttc taaaatagta aaacgcgatt tataccacga aaaacaatgc cgtttcaagt
    23401 gttttaaaga aaaacagatg ttttgcgcgg gtaagccgcc cgagtgccgc atctgcggaa
    23461 cgtttgcagg tgcgaaacag atggggcgcg attatagcgg atttcgggag cttctgcatt
    23521 caaaatgccg tccgaacata tttccagtat tttcagacgg catttcaagt tttcaaatcc
    23581 ctcaatgtcc accgccgccg ctgtggaacg gcggtttcgc cagccagaca atgggaatca
    23641 ggacgataaa caaaatgctg ccggcgagga atatttcgtt cgatccgatg atgaagccct
    23701 gttgcgtaat ggtattgttg atgatgccta gagtttgacc gtcggaaatg ccttgttggg
    23761 acaaatgcgc cgccgtttcg tgcaatgttg cggaataggg cgtgatgtgt tcggcaaaac
    23821 gcgtgtggtg caacgcttcg cgccgttccc acagggtgct gacgacggat acgccgacac
    23881 cgcccatcag cacgcgcagg aaattcgaca ggctgccggc ggcggcaatc tgtccgccct
    23941 tcatgtgcga cagtgtgatg gtggtcagcg gcaggaagaa catggcaacg cctaccccct
    24001 gccaaaactg cggccagatg acgttgccga tatccatgtc ggcataaaaa tcagtgcgcc
    24061 aataaaaagt gaaggcaaag gtcaggaagc tggcggttac gagcaggcgc atatcgattt
    24121 tattgccgaa cctgccgatt aacggagata ggaagacggg gaggatgccg acgggcgcgg
    24181 cagcaagccc tgcccacgcg gaggtatagc ccaagttggt ctgcaacact aacggcagca
    24241 gggtcagcgt ccccatatac accatgaaac ccaacgaggt ggcaattacg ccgacggtaa
    24301 aattccgatc tttaaacagc gataaatcga caatcggata tttttctccc aattcccaaa
    24361 caataaaata cgacaagcac accagtgcga ctacgcccaa ggtaatgatt tctccagagg
    24421 cgaaccagtc aagttcctta cccctgtcca gcatcatctg taacgcgccg ataccgacca
    24481 tcatcagcgt cagcccgaca tagtcggtcg gcgttctgac ggttgccgtt tcgcggtgtt
    24541 tcaaatgttt ccatgtaatc catgccgata tgatcccgat ggggatatta atgaagaaaa
    24601 tccaacccca atgccagttt ccggaaatcc aaccgccgag tatcggcccg aggacagggg
    24661 cgacaacgac ggtcattgcc cacaatgcca gtgccagcat ccgttttgcg ggcggatagg
    24721 atgccattaa caggctttgc gacaggggaa tcaacggccc ggcgataaag ccctgcaaaa
    24781 tgcggaaaac aaccagcgac tgaaggttgg gcgcgatgcc gcacagccac gatgcgatga
    24841 cgaaaccggc ggcggcggcg gtaaacaatt tgacctcgcc gatacgtttt gccaaaaagc
    24901 ccgtcagcgg cacggaaacg gcgtttgcca cggaaaaaga agtgatgacc cacgtcccct
    24961 gcgtggttgc cgcaccgagg ttgccggcaa tgacggggac ggcgacgttg gcgatggtcg
    25021 tatctaaaac ctccataaat acggcaagcc ccaaagacag cgtaacccac gccaatgccg
    25081 cacccttaag cggtggataa tccattgtgt cctccgtttc agacggcatc agggcgcgga
    25141 ttgcccgagg atttcatcga ccgtccgatc gacttccgac cagtcggtac tttccatttc
    25201 cggcaatgcc gcacccggcg tttttgaaac aggcgcgcct gcggcggaag tatccacttt
    25261 aaccgtcatc gacaaaccga tacgcaacgg atacctgtcc acatcttcgc ggttcagcac
    25321 gatacggacg gggacgcgct gcaccacttt aatccagttg cccgttgcgt tttgcgccgg
    25381 aatcagcgaa aacgcgctgc ccgtacctgc cgaaaaccct gccaccctgc cgcgataaac
    25441 aatttgtttg ccgtacaaat cggacaccag ctcggcaggc tgtccgattt tcatatgccg
    25501 caactgcgtc tctttaaaat tggcatccac ccacacatcc gacagcggca ccaccgccat
    25561 cagcggcgcg cctgccgcca cctgctgccc gacctgcacc gaacgcttcg ccacctgacc
    25621 gtccgccggc gcgcggactt gcgtccgccg aaggttcagc cacgcatctt tcaacctgcc
    25681 gattgccgtc tgaacctccg gctgttcgcg caaagaaaca tctccgccca aagccgcgcg
    25741 tgccgaagat tcttccgcca aagccgcctt aaccgccgcc tgcgcctgag acactgccgt
    25801 acgggcgtgt gccagctctt cggcggacac cgcacccgat tccgccaaag cggaccggcg
    25861 gcgcaaatca tcctgcgcgc gcgccaaatc cgcacggcgc aaggcaacct gcgcccccgc
    25921 ctgcgaagtg gcggcatttt gccggcggtt ttggcgcacc gcctgaacca actcgttttt
    25981 tgcccgctcg taagccagca catcattatc gtcatccaac accgccagca cgtcgccttt
    26041 tttcacggca tccgtatcgt cgtgcaaaac cttccgcacc gtgccgccct tttgcggcgt
    26101 aacctgaacc acgcgtccgg caacataagc gtcttccgtt tcctcttcgt gctgccacca
    26161 taaaaaaaac gccgatccgg cggctgcggc ggaaagcgcg aacagcagcg tcaatgccgt
    26221 caggcggcgt ttgcgtttga cttgcgtgtt ttgaagtttt gtttcgtctg tgtgcgcttc
    26281 cataagattg ggttccgaat ttcctgattt catataaaca atattctttc aataggaaat
    26341 agattttata tccgcgcaat aacgcaatca agtcagtatt ttagatgcaa tattcaaaaa
    26401 acaacgcttc
//
LOCUS       DAXFZR010000019        25505 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000019 DAXFZR010000000
VERSION     DAXFZR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25505
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.019"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..155)
                     /locus_tag="ACFJAS_000696"
     CDS             complement(<1..155)
                     /locus_tag="ACFJAS_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC3982006.1"
                     /translation="MSTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYLNRQKTR
                     YLRDRRGR"
     gene            complement(295..937)
                     /gene="pgmB"
                     /locus_tag="ACFJAS_000697"
                     /pseudo
     CDS             complement(295..937)
                     /gene="pgmB"
                     /locus_tag="ACFJAS_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
     gene            complement(950..3208)
                     /locus_tag="ACFJAS_000698"
                     /pseudo
     CDS             complement(950..3208)
                     /locus_tag="ACFJAS_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            complement(3391..4352)
                     /locus_tag="ACFJAS_000699"
                     /pseudo
     CDS             complement(3391..4352)
                     /locus_tag="ACFJAS_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(4356..5710)
                     /locus_tag="ACFJAS_000700"
                     /pseudo
     CDS             complement(4356..5710)
                     /locus_tag="ACFJAS_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
     gene            5946..7622
                     /gene="ettA"
                     /locus_tag="ACFJAS_000701"
     CDS             5946..7622
                     /gene="ettA"
                     /locus_tag="ACFJAS_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC3982007.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            complement(7682..8167)
                     /locus_tag="ACFJAS_000702"
     CDS             complement(7682..8167)
                     /locus_tag="ACFJAS_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC3982008.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(8160..9116)
                     /gene="thiL"
                     /locus_tag="ACFJAS_000703"
     CDS             complement(8160..9116)
                     /gene="thiL"
                     /locus_tag="ACFJAS_000703"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC3982009.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            complement(9891..10601)
                     /gene="rmpM"
                     /locus_tag="ACFJAS_000704"
     CDS             complement(9891..10601)
                     /gene="rmpM"
                     /locus_tag="ACFJAS_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC3982010.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPTRNHHQH"
     gene            10965..11915
                     /locus_tag="ACFJAS_000705"
     CDS             10965..11915
                     /locus_tag="ACFJAS_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC3982011.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(12028..12762)
                     /gene="fnr"
                     /locus_tag="ACFJAS_000706"
     CDS             complement(12028..12762)
                     /gene="fnr"
                     /locus_tag="ACFJAS_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC3982012.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            12897..14318
                     /gene="hemN"
                     /locus_tag="ACFJAS_000707"
     CDS             12897..14318
                     /gene="hemN"
                     /locus_tag="ACFJAS_000707"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC3982013.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPGDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(14356..14544)
                     /locus_tag="ACFJAS_000708"
     CDS             complement(14356..14544)
                     /locus_tag="ACFJAS_000708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982014.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(14558..16132)
                     /locus_tag="ACFJAS_000709"
     CDS             complement(14558..16132)
                     /locus_tag="ACFJAS_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC3982015.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            16457..17557
                     /locus_tag="ACFJAS_000710"
     CDS             16457..17557
                     /locus_tag="ACFJAS_000710"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC3982016.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            17676..18617
                     /gene="mafA"
                     /locus_tag="ACFJAS_000711"
     CDS             17676..18617
                     /gene="mafA"
                     /locus_tag="ACFJAS_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC3982017.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPDSATRYSYPA
                     YDTTATTKSDALSGVTTSTSVLNAPAAALTKNNGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            18621..20438
                     /gene="mafB"
                     /locus_tag="ACFJAS_000712"
     CDS             18621..20438
                     /gene="mafB"
                     /locus_tag="ACFJAS_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC3982018.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYNVNGTARSIKLNPTDTRDVRQRISDNYSNLGSNFSDRADEANRKMFEHNAKL
                     DRRGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AAIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKA
                     AVSGDFSKSYTCSFHGSTLVKTADGYKAIAHIQAGDRVLSKDEASGKTGYKPVTAQYG
                     NPYQETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSESGKTQTVR
                     NTVVKPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPTKLKPTERYNRQTHYGG
                     SQTDGARAQAARQAGEGKPCPTCGRIQIFGTKTAPSPQHEPPLVKHYYEHGGHSMSNA
                     DRAKHARESIKGTQCLTCQRKEGAMMSRYSREQAKKHGL"
     gene            20428..20976
                     /locus_tag="ACFJAS_000713"
     CDS             20428..20976
                     /locus_tag="ACFJAS_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC3982019.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            21273..21626
                     /locus_tag="ACFJAS_000714"
     CDS             21273..21626
                     /locus_tag="ACFJAS_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982020.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            21616..21939
                     /locus_tag="ACFJAS_000715"
     CDS             21616..21939
                     /locus_tag="ACFJAS_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC3982021.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIDGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            21998..22225
                     /locus_tag="ACFJAS_000716"
     CDS             21998..22225
                     /locus_tag="ACFJAS_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982022.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            22352..22895
                     /locus_tag="ACFJAS_000717"
                     /pseudo
     CDS             22352..22895
                     /locus_tag="ACFJAS_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            23030..23761
                     /locus_tag="ACFJAS_000718"
     CDS             23030..23761
                     /locus_tag="ACFJAS_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC3982023.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYR
                     ETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSESGRTQTVRNTVV
                     KPKPLKAYNLTVADWHTYFVKGNRAETEGVWVHNECPYGKGNQRYKDAPYHGKNDNSV
                     KSRAPTNGQAVLDNSVQVKSTSSQRVGVDKTNNEIVVLNQTRIFNDGSAEYHGHVRNW
                     KNLHTDQQNALKKAGLVNSKGKIKK"
     gene            23758..24093
                     /locus_tag="ACFJAS_000719"
     CDS             23758..24093
                     /locus_tag="ACFJAS_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982024.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            24141..24665
                     /gene="mafB"
                     /locus_tag="ACFJAS_000720"
                     /pseudo
     CDS             24141..24665
                     /gene="mafB"
                     /locus_tag="ACFJAS_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
     gene            24643..24924
                     /locus_tag="ACFJAS_000721"
     CDS             24643..24924
                     /locus_tag="ACFJAS_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982025.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            24980..25505
                     /gene="mafB"
                     /locus_tag="ACFJAS_000722"
                     /pseudo
     CDS             24980..25505
                     /gene="mafB"
                     /locus_tag="ACFJAS_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
BASE COUNT         6764 a         6727 c         6319 g         5695 t
ORIGIN      
        1 cggccgcggc ggtctcggag gtaacgggtt ttttgacggt tcaggtattg ttcgatcggc
       61 tgccaatcaa tcacctggtc caactccgat agcgggaagc ggccgatgtg tttggcagtc
      121 atggcttgtg cggtttgccg gaagaaggtg ctcatgggaa atcccctaaa tgccttggtg
      181 ggaatttagg ggattttggg gatttttgca aaggtctcgg gttatcattc tgttctaaaa
      241 gcagaaaggc cgtctgaaac actgttcaga cggccttttt acttccttag aattttatct
      301 gcccgactgt tcccacacat tttgcaagta ggcgtaggtc agctcggcgg tgtcggacac
      361 cagcgcgatg tcgctgccca agtcttcggt tttgcccacg ccgatgggca aagcgccaga
      421 ggctttgatg gcggcgacgc cggcagcggc gtatgtccgc acccacgccc tcggcggcgg
      481 caaggaagat gtcgggggag ggtttggaat gcgcgacggc ggcagggtcg gcgacggtgt
      541 cgaagaagtg ggtcagcccc atgcgttcga gcaagaacgg gccgttttta ctggcggacg
      601 cgagggcgat ttttttgccg tttgccctca atgcttccag cagcggcaaa atgccggggt
      661 acacgtcttc gggtttgact gcctgaatca tctccaagta gttgtcgttt ttgcggcagg
      721 tcagttcggc gaactcggct tcgccgacgg ttttgccgcc gtgcgcgagg atgcgtttga
      781 gcgaatcgtc gcgcgacacg cctttgagct gctcgttaaa cttgcggtcg atgccgatgc
      841 ccagttcttc ggcgagcttt ttccatgcga ggtagtggta ttcggcggta tcggtaatca
      901 cgccgtcgag atcgaagagc actgcggtaa aagtcatttt gcgccctcct tatttttcca
      961 atgcaacggt gtggctgccg ttgagcgtga tatctttgcc gtacacctgc aattcgagcg
     1021 gatcgccttt gagcagggtg aagacgacgt ttttttgccg acggcgactt taatcagacg
     1081 gccgcggtag ttgatgtgga aggcatagcc tgtccacgca ctcggcagga acggtgcgaa
     1141 gctgagtttg ccgccccaag ttttcatttg ggcgaaacct tggacgatgg cgagccacga
     1201 gccggtcata gaagtaatat gcaggccgtc ttcggtgtcg ttgttgtagt cgtccaagtc
     1261 caggcgggcg gtgcgccggt acatttccac ggctttttct tctttgccca gttcggcagc
     1321 gaggatggag tggatacatg gagacaacga gctttcatgc acggtcatcg gttcgtagaa
     1381 gtcgaagttg cggcgttttt cgtccatgtc gaaacggtcg ccgaagaagt agatgccttg
     1441 caatacgtcc gcctgtttga tgaagggcga acgcaggatt ttgtcccacg accatttctg
     1501 gttgagcggc aaatcgtcgg gcgaaagcgc ggatacgggg cggatgtctt tgtcaaggaa
     1561 gccgtcgtgc tgcacgaata cgccgagttc ttcgtcatgc ggacggtaca tattcgcgct
     1621 gatgtccgcc catttttcca actcggcggc gctcacgttc gaacccggac gcgggtattt
     1681 cgccaaggct tcgcgggtgt agcccaatac ccatgcggcg agggtgttgg tttaccagtt
     1741 gttgttgatg ttgttttcgt attcgttcgg accggttacg ccttgaatca tgtatttgcc
     1801 gttgcgtttg gagaagtgga cgcggtccgc ccggaagcgg gacacttcga ccaagacttc
     1861 caagccttct ttggcaagat agcactcgtc accggtgtag ttggtgtagt tgtagatggc
     1921 gtaaggaatc gcgccgttac ggtggatttc ctcgaaggtg atttcccatt cgttgtggca
     1981 ctcgatgccc gtaaacgtta ccatcggata gagtgcgccc gccaagccct gttcgcgtgc
     2041 gttatgctgc acctgcggca atttggttgc ggcggtattg cagcaggttg cgggtaactt
     2101 cgggttccgc cagtgcaagg tagagcggca cggcgtaggc ttcggtgtcc caataggtcg
     2161 cgccgccgta tttttcgccg gtaaagcctt tggggccgat gttcagacgc gcgtcttcgc
     2221 cgtagtaggt ggagaacagt tggaacaggt tgaagcgaat gccctgctgt gcttcgtcgc
     2281 tgccttcgat gaccacgtcg gcgatttccc aacgatgctg ccagcctgct ttgtgcgcat
     2341 ccagcaaggt ttcaaacgca acacctgcga ttttttcaga taaggcgcgg ccggcggctt
     2401 tcactgcttc caagccctga taatcgcggc tggtggtaac aatcacgcgt ttttcaaagg
     2461 tttcgggcgt gccgccgact tcggcttcaa aagaattaga gacctgccaa tcggtttggc
     2521 tgccgccgaa ggctttgaag ctgccggcga aggtttgccc ggcgttgacg ataaattgtt
     2581 ccacaccgaa agggttggcg acggtttggg cggcaatgta ggagcggtcg tctgaaacgc
     2641 ctttgtccaa tacctgccag aatttttctt cgtagttgga gtcttcgttt ttcacgtcgg
     2701 catcgatgat ggaatcgatg cggacttggt gggttttgcc gtcaacggac acggcttccc
     2761 agcgggtgag agccagctct ttttgcgcga ccgacaggaa tttgcacaca tcgaaacgca
     2821 caccgaatac ggtgaacgag cggcgcaaca cgccgtgttg catatccagt tcgacggaga
     2881 agccggcaac gtcgtttttc gccaagtcca cctcttgccc gtcgacaaag attttgactt
     2941 tgctgaaatt gagcgcgttg atggctttgc cgaaatattt gggatagccg tttttccacc
     3001 agccgacgcg ggttttgtcg ggaaaccaca cgccggcgat gtaggtgccc aagtggctgt
     3061 cggcggagta ggtttcctca aagctgccgc gcatacccat atagccgttg cccaagctgg
     3121 tcaggctctc ttgcagccgt ttgtgttctt tttccagttt tgccgaacgc agcgtccaag
     3181 ggctgatttc cataattctt gtgtacatcg taaagctcct gtttggattg atttgaggga
     3241 atggtgaaat cttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
     3301 tacagatagt acggcaaggc gaggcaacgc cgtactggtt tttgttaatc cactataaaa
     3361 aggtcgtctg aaaccggtgt taggaagctc ctaagaaagg gattcgatgc cgtaagcaat
     3421 cgtcgcctcc ctagtatcac ccttgttcag acggatattg ccgaattcgg gccaatgcag
     3481 gctgtcgggc agcgtctgcg cctcggtcgc cagcgcgtcg taaacgcccg catcgtgccg
     3541 cgcgaaatcc tgcggggcgg cggtaaagat gaccaagccg ttgcggtcgc tgtatatgct
     3601 gatacgacgg cggcgtccgg cttgcaacac ggcggcggga cggcctatat cggacggcac
     3661 gcggtaagcg tcgtcaaaac cggcccgttt cgcggcgcag ggcggcaacg gcggcatccg
     3721 gcggcttgga ccggctgaaa tcaaatactt cgaggccgtc tgaaaccgtt aagacgggca
     3781 gtttttcggc atcggccgga atatgtccgc cctgcggaat atgcagaacc gcatcgtgca
     3841 ggcccgcgtc cagccgccag taaatgtgca gcgtcgggtc gaacaccgtg tcgccgagcg
     3901 cggtggcgcg ataggtaacg gtaagccggc cgtcctcgtc caagcggtag gaaatatcca
     3961 aatcgttggg ataaccgtcg gccgtctgcc gccaccgcgt tgaaacgggt aacggccagc
     4021 ccgtgcgaac cgccgtgcag cgcgttcctg ccttcgttgg cctccacgcg gtaagtccta
     4081 ccgttgatgt cgaacgccgc accgcggatg cgtccggcca cgcgccctat ctgcttgtta
     4141 atctgaaacg gattgtccgc ataggaagcc gcatcgtcga acgacaccac ggggttttcg
     4201 cgcacgccgt ctgccaaaac ggaaaattcc tgcacaatcc cgcccaagtc cagcacgaag
     4261 acgcacgtac cacgccggtt ggacagcaca tagccggtta cggcccgccc gtcgatcagg
     4321 ccgaaatcgc gggtagcggg ggtatcgctc atcgctcaaa ccccgccgtg ggtctcttta
     4381 atcagacaga ctgagaaggc tcccagcagc aagactgcgc ctgcaaccaa gaacatggtt
     4441 gcctgatggc cgcccagcat cgggaaaagc acgaaactca acagcgaagc gacgatttgc
     4501 ggcatacaga cagagccgtt aaacaggccc aaataagtat ccatgtgttt gcccgacaaa
     4561 gcgttggcca caatcgtcag cggataagtg ataatgcccg cccaagcgat gccgattaag
     4621 atataagaca ggatgagtgc gtattgattg tagatgaaga agatagagaa gaaaccgagc
     4681 gcgcccaaag ccaaacagcc gaaataaccc gccttatggt atttattcgg tacttttgcc
     4741 agaataaacg aacaaatcac cgccgcaacc gactacaccg ccgccaaaac gccgtaccgg
     4801 ttgcccgcct cctgatggcc tacggaagac gcatcggtag tgtgccagac gttttctgca
     4861 atcgcgcctg ccgagtaagt ccacatatac cggaaggcga accagcagaa aaactgtacc
     4921 ggagtaaccg tccaaaacac tttaggcgcg gtttttaaga gttcgaacca gttggctttt
     4981 tcctgattcg cggcgacatc gatgccgtgg taacgggcgt aggtttccgg gtcgtattct
     5041 ttgactttgg agattgtgaa cgcactggta ataatcagta acgccgcacc cacatagaat
     5101 gctacgacca cggtttgtgg cacaacgcct ttctcggcag tgttcgccaa accgatatac
     5161 gcgaacacaa acggcagaat cgctgccaca accgcgtccg tattcgctaa gaaactttga
     5221 atcccgtagg cgtagctttt ctgctcctcg ttgaccatat cgccgaccat catcttaaac
     5281 ggctgcatcg ccatattcga cgacacgtcc aacagcgcaa tcatcagcgc gccgaacgac
     5341 aaggccgcca gcgacgcata gccgaaaccg aagctgcccg agttcggcat caaaatcatc
     5401 acgatgaccg caatcagcgt gccgtaaagc agatacggca ggcggcggcc gcccaagcgc
     5461 ggcttccaag tgcggtctga gtagtagcca ctatcggctg aaccagcatc cccgccagcg
     5521 gcggcaggat gaaaaaccag cccaaattgt gcgggtctgc gcctagcgtt tgaaaaatgc
     5581 ggctcatctg cgagctttgc agggtaaagg ccgtctgaac gccgagatag ccgaagctca
     5641 acatccaaat cgtgcttttt gccggcgcgg gcaaaccttg ttttgctgtt tgaggcgtat
     5701 attccgacat aaagtaaatc cttttttttg atttgaaaag tatttgcttt ggaaaatccg
     5761 aaatggttgc cggcgcggcg atcccctatc attattattt ttttgtctgt ataatttcaa
     5821 agggataggc ggattttatg aatcctgccc gattttggca ataccggttc gcggataaac
     5881 tggcttaaat caaattatcg gttaaaatgg ccgtctgaaa tttgtttgac cgaatcgaga
     5941 aaactatgtc ccaacaatac gtctattcta tgctgcgcgt gagcaaggtt gtgccgccgc
     6001 agaaaaccat cattaaagat atttcccttt ctttcttccc cggcgcgaaa atcggcctct
     6061 tgggcttgaa cggcacgggc aagtccaccg tgctgcggat tatggcgggc gtggataaag
     6121 aatttgaggg cgaagccgtg ccgatgggcg gcatcaaaat cggctatctg ccgcaagagc
     6181 cggagcttga tccggaaaaa accgtgcgcg aggaagtgga aagcggtttg ggcgaagtgg
     6241 ctgccgcaca gaaacgtttg gaagaagtgt atgccgagta cgccaatccc gatgcggatt
     6301 ttgacgcgct ggcggaagaa caaggccgct tggaagcgat tatcgcggcg ggttcgtcaa
     6361 ctggcggcgg tgcggaacac gaattggaaa tcgctgccga cgcgctgcgc ctgcctgatt
     6421 gggatgccaa aatcggcaat ctttcgggcg gtgaaaaacg ccgcgtcgct ttatgcaaac
     6481 ttttgttgag caagcccgat atgctgctct tggacgagcc gaccaaccac ttggatgcgg
     6541 aatcggtgga atggctggag caattcctcg tgcgcttccc cggcacagtg gtcgcggtaa
     6601 cgcacgaccg ctacttcctc gacaacgccg ccgaatggat tttggaactc gaccgcggac
     6661 acggcattcc gtggaaaggc aattactcgt cttggctgga gcagaaagaa aaacgcttgg
     6721 aaaacgaggc gaaatccgaa gccgcgcgcg tgaaggcgat gaagcaggaa ttggaatggg
     6781 tgcgccaaaa tgccaaaggc cgccaagcca agcccaaagc gcgtttggcg cgttttgaag
     6841 aaatgagcaa ctacgaatac caaaaacgca acgaaactca ggaaatcttt atccctgttg
     6901 ccgagcgttt gggtaacgaa gtgattgaat ttgtgaatgt ttccaaatcg ttcggcgata
     6961 aagtgctgat tgacgatttg agcttcaaag tgccggcggg cgcgattgtc ggcatcatcg
     7021 gcccgaacgg cgcgggtaaa tcgacgctgt tcaaaatgat tgcgggcaaa gagcagcccg
     7081 attcgggcga agtgaaaatc gggcaaaccg tgaaaatgag cttgattgac caaagccgcg
     7141 aaggtttgca aaacgacaaa accgtgttcg acaacattgc cgaaggtcgc gatattttgc
     7201 aggtcggaca gtttgaaatc cccgcccgcc aatatttggg acgcttcaac tttaaaggca
     7261 gcgaccaaag caaaatcgca aggcagcttt ccggcggcga acgcggccgt ctgcacttgg
     7321 caaaaacctt gttgggcggc ggcaatgtgt tgctgctgga cgaaccgtcc aacgatctcg
     7381 acgtggaaac cctgcgcgcg ttggaagacg cattgttgga atttgccggc agcgtgatgg
     7441 tgatttcgca cgaccgctgg tttctcgacc gcatagccac gcatatcttg gcgtgtgaag
     7501 gcgactccaa atgggtgttc ttcgacggca actatcaaga atacgaagcc gacaagaaac
     7561 gccgactcgg cgaagaaggc gcgaaaccga aacgcatcaa atacaaaccg gtaacgcgtt
     7621 aacctccgaa acaatgccgt ctgaaaggct ttcagacggc atttttacaa ggcagccccg
     7681 tttaaaacag cattgcaatc ctcaagacaa tcaaggtcat caccgcagcc gccatatcgt
     7741 ccgccataat gcccaaaccg ccgtgcagat tcttgtcaaa ccaaccgacg gggggcggtt
     7801 tgagcgcatc aaacagacgg aacagaacaa atgccgccag ccaccacgtc cacctgaacg
     7861 gcacaaacgc cagcacaaac agcatggcga caatctcgtc ccaaacaatc ccaccgtggt
     7921 cactgacacc cgtttcacgt tccgcacacg cacaaatacg tatgccccac ataaacagca
     7981 cgatacacac aaaagccagc agcagcccgt ctatgccgag caaaatcagc acaaacgcca
     8041 aaggcagtgc cgccaaagtg ccgaatgcgc ccggcgcaaa cggagccagc ccgctgccga
     8101 aaccgaaagc caaaaaacac aacggccgtt tcaacagcca cgcaaagtca ggtttaaaat
     8161 cagccaaaat gatcgaatcc taaagaatgt agttccaatt ccctgccgcc ggcatctaaa
     8221 accttcagac ggcatcctcc gttgattttg ccgatgcacg ttaccggcac gccgcaccgt
     8281 tccgccgcat caagcacgcg gctgcggcaa ctttccggag cggtaaaaac cagctcgtaa
     8341 tcgtcgccgc ccgccaaagt ataagacagc cattgcgctc ggggcaaaat atctttcaat
     8401 acagataaag atggcagcga atcggcccaa atttccgccc ccacgccgga agcggtcagg
     8461 atatgcccca aatcttgcgc aaggccgtct gaaacatcct gtgccgcccg ggcaaacggc
     8521 aacagcgcaa gccccagccc aacccttggc tcgggacgga gcagcttttg ttcgcattcg
     8581 gcgaacacat catccggcaa cacacaccgt ttcagacggc agttcaaagc cgccgccgcc
     8641 ataccgacac gccccgacac ccaaatatcg tcgcccgcaa ccgccgcatc acgccgcaac
     8701 gccctaccct tcggcaattc gccgataatg gttacattga acgccatatc gcccttggtc
     8761 gtatcgccgc cgattaacgt tacgccaaac ttttttgcca aaccgaaaaa gctgccgcaa
     8821 aaccgttcca gccatacttc atccaattcg ggcaacgccg cgctcagcag cacccaacgc
     8881 ggtatcgcac ccatcgccgc catatctgaa atattgacgg ccaaaacctt ccaagccaag
     8941 ccttcaggtt tgacatctgc aaaaaaatgc ctgtccttca aaagcatatc cgcactgaaa
     9001 cacaaatcga agccttcgcg cgggcggaca atcgccgcat cgtcgcctat gcccaatacg
     9061 acatccttat ccgtacctgt ttgcaaatac cgtttgatga agtcgaattc tttcatatcc
     9121 caaaatatcc ggcgtgcgaa tccaatttaa atcatagcaa agcagcaaag gtcgtacaaa
     9181 caataaagcg gctcattcag tgtttcagcg tatttcactt ttagctttag ccataccgta
     9241 cccgtatgca ccgaccaccc tgtttcaaca cacaaacggc atatcaaagc accgcactgc
     9301 acgccgcccc gaatgcaaag cggcaacttc cgccaccgat taggctcaat cgctgcaact
     9361 ggcggggttt caaccgaaaa gggatgatga taaaaccata tccggcatac agtgccgcac
     9421 acatcgggca cgattccatc catttcccaa tgagggggaa aggcgtgaat ttgtatgaat
     9481 tatccctttc ggggcttgat acagaaagga ttcccttgct atgggcaaac ccacatccta
     9541 ccgccttaaa atcagagttt caaccgatat agtggattaa atttaaaccg gtacggcgtt
     9601 gcctcgcctt gccgtactat tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
     9661 taatccacta taaagcgcgg caatgggaca caaacatgaa tctgaatccg aatccgaacc
     9721 tgcaccccga ttctgacgat agccgtttca gaaaaccacc caaaaaaaca aaccccgcaa
     9781 aagcggggtt tgtttcacat catcgatacc ggccgacgtt tcacgaatat tgcaacagta
     9841 cccggtacaa aatccgctaa cctcatgcat cggcaagata ttacctagcc ttagtgttgg
     9901 tgatgattgc gtgtcggcac aacctgacgg gttacgatgc tgcggatttt cacatctacg
     9961 cggcggtcag gttcgataca tgcaatcaga gcctcacgtt ttttggcttt agaggctttc
    10021 gcacccagtt tggcaacttc ggcttgacaa acttgagtca tttgcgcttg agattcgccc
    10081 aagccgacag cagaaattct agaagcaggt acgccgttgc tgaccaggtt gtttgccact
    10141 acgtatgcgc ggcgttcgga cagagcctga ttgtattttt cagaacccat aaagtcggta
    10201 tggccttcga cgcggacaga ttggacattg gttcgactca ggcgttgcgc caatactttc
    10261 aggttgtctt gagcttcggc gcgcaatgaa tccttatcga aaccgaacag ggttttggca
    10321 gacagggaaa tggtttcatc aacatattga ggagcctgct ccacaacggc gacaggcgcg
    10381 ggttcgggct cggggacggc aaccgcatcg ccgcattcta cgcgaccttg gcttgcttta
    10441 tcaaagtagg cgtttttcca gcattctcca tagttgttgc gtacgatttc gttcgattgg
    10501 ccgcttacgg tgtaaccctg aacggacgcc tcgcccgcaa cagcagtgcc ggaagcgagc
    10561 aatgcaacga ataatgcgct taatttcagc tgtttggtca ttttattccc tcattagatt
    10621 tgtacagcag atacagaagc aactgctgaa ttgccaacat acaggccgct gtagaaacat
    10681 ggcggcaaat cggatatttc agtctccact ataaagaaac agggggcgga ctgtcaatac
    10741 tcaacgcctg aaaacaaccg taaaacagcc tgtttcctga tagtctatca tgccgaaaaa
    10801 tcaattccgt gtcattactt tagggggatt ttttccatat cgcacgattg ccgttgcaca
    10861 aaaacaacaa acacaacggc aaagccccat accgtaccgg caagaaaata tgataaatta
    10921 taaacaatgt tcagccaccc gacacagacc cacaccgacc cgccatgaaa ttacaacaat
    10981 tgaaatacgc cttagaagtt taccggcaca acctgaacgt ttccgaagcg gccgaagcct
    11041 tattcacatc acaacccggc atctccaaac aaatcaaatt gctggaagaa gaaatcggca
    11101 ttcagatttt tatccgcagc ggcaagcgcg tggtttcggt ctcgcagccg ggcaaggtgg
    11161 ttttggatat tgcggaacgt attttgcgcg atgtacagaa cattaaaaat atcggcagcg
    11221 agtttaccgg acaggacagc ggttcgctga cggttgccac gacgcatacg caggcgcgct
    11281 atgccctacc gttgattgtt gccgattttg tgaaacgcta tccgaaagtc aatctgacca
    11341 tcaaacaggg aagccctgcc gccatcgccc aaatggttac ttcgggagaa tctgatttgg
    11401 cgattgttac ggaacggata gacgaccacc ccgagttggg aaggctttcc tgctacgact
    11461 ggacacacgc ggtgattgtg ccgaacgacc atccgctgct cgaatgcagg aatcccctcc
    11521 gtattgaaga tttggcgagg tttccgctga ttacttatga atttgcattc aatgcgggca
    11581 gcagcatcgc gcgggcattc gccaaagccc gtttggaacg gccggatgtc gcattggcgg
    11641 cggcggatac ggatgtattg aagacttatg tgcgcttggg tttgggcgtg ggactgatgg
    11701 cgaaaatggc atacaacccg gatacggacg gcgatttgca gcttgtggat gcggcacacc
    11761 tgttcgagcc gtcgccgacg tggatagctt tgcgcagcga tacttatttg cgcggatatg
    11821 cctacgactt tatccaagcg tttgcgccgc acctgacacg cgagaaggtg gataggattc
    11881 tgtacacgcc catcagcgag gatttttcga tttaggcggc tgccggtttt caggcggcac
    11941 tttgcggcag atacaacaaa cagggcagat gttttcgtct gccctgtgtt tattgagaat
    12001 gccgtctgaa atgttcgtac aggttaatca aatggcgtgc gaacaaccgg ataccatttt
    12061 tttcagcacc tgcagattga gaatcttgat gtgtttgtgt tcgacggaaa tcaatccttc
    12121 ctgatggaac ttggataatg tgcggctgac ggtttcaagt ttcagcccga gataactgcc
    12181 gatttcttcg cgggacattc ttaagatgaa gtcgttggcg gcgaaacctc gggaataaag
    12241 gcgttgggaa aggttcaaca ggaaggcggc aatccgctct tcggcgcgca tattgcccaa
    12301 caacagcata acaccttgat cgcgcacaat ttcgcggctt atcatgcgga aaaagtgtgt
    12361 acgcaggctg gggatattct gaccgagctc ttcgatatgg gtaaacggca actcgcatac
    12421 ctcgctgttt tccaaggcga ccgcgtcgca actgtgcaca taggaacaga tgccgtccat
    12481 gccgatgagt tcgcccgaca taaagaaacc cgttacctga tcgcggccgt cctgactggc
    12541 gacggttgtt ttgaagaagc ccgaacggat ggcaaagagc gaggtaaagg ctccgccggc
    12601 acggaacagg tattcgcctt ttttcaggcg gcggctttgg cggatgacgg catcgagttg
    12661 gctgaactcg ttgggcagca gcccgacagg caggcagagt tcccgcaaag aacaggaaga
    12721 acacagcgtt ttcatctgat gtgtagtatt atgcgaagcc ataccgtacc ttttcgtgtg
    12781 ttttgcccca tcccgattat gtgcttgttg acacatatca agacaggttt atcatactgt
    12841 ggcattctac caaactatcc aaaatttgac taacatcata aaaccgcaca cacaccatga
    12901 aaatcattca gatacagaac aatcacaatg taaacgatga ccgccccgag tttgaccgcg
    12961 cgctgattgc cagcctgccc gccagcggcc cgcgctatac ttcctaccct accgccgacc
    13021 gtttccatga cggtttccgc gaaggcgaat atatcaaagt tttacatttg cgcggtatgg
    13081 gcgcgttaaa caaaccgctt tccctttaca ttcacattcc gttttgcaac accatctgct
    13141 actactgcgg ctgcaacaaa atcatcacca aagacaaaag ccgcgccgat acctacatcg
    13201 aatatcttga aaaagaaatg gaactgctcg ctccacatct gaacggacgg caccagcttg
    13261 cccaactgca cttcggcggc ggcacgccga cctttttgag cgacgaacag atcgaacgtg
    13321 tcttccgcat gatccgcaaa catttcgaac tgataccgtc cggcgaatat tccatcgaaa
    13381 tcgacccgcg caaagtcagc cgcgacaccg tcctcatgct cggcagactc ggcttcaacc
    13441 gcatgagcgt cggcattcag gatttcgacc ccaaagtgca ggcggcagtc aaccgcatcc
    13501 aaagctacga agaaaccaaa gaagtcatcg atgccgcccg tgaggcaggg ttcaaatccg
    13561 tcagcgtcga tttgatttac ggcttgccgc atcagacttc cgaaagcatc aaaaccacca
    13621 tagataccgt tttgtcgctc gatcccgacc gcctcgccct ttaccactac gcccacctgc
    13681 cgcacgtgtt caaaccgcaa cgccgcatcg ataccgccgc cgtccccgac agcgaagaga
    13741 agctcgatat gctgcaatac tgcgtccaaa ccctgacaga gcgcggctac gtcttcatcg
    13801 gcatggacca tttcgccaaa cctggcgacg aactctccat cgccctcaaa gaaggcttcc
    13861 tccaacgcaa cttccaaggc tattcgacct acgcggattg cgatttggtc gccatcggcg
    13921 tgtcgtccat cggcaaaatc ggcagcacct attcccaaaa cgaacgcgac atcgatgcct
    13981 actatgccgc catcgacgaa ggcagactgc ccatcatgcg cggctaccag ctcaatcagg
    14041 acgacatcct gcgccgcaac atcattcagg atttaatgtg ccgcttcgcg ctcgactaca
    14101 ggatttacga aagtatgttc ggcatcccgt tcgaccgcta cttcaaagac gaactggcgg
    14161 atttggaaaa actcgccggt ttgggattgg tgcgcctgaa cagccacggg ctgaccgtta
    14221 caccgaaagg acgcttcctc atccgcaaca tcgccatggt cttcgactac cacctgcgcc
    14281 acaaagaaac caaagcgaaa tattcgcaaa cggtgtgatt gcggctaacg tacaaaagcc
    14341 gtctgaaagg ctttttcaga cggcattttg ctgccgagga taagtgtttt caagaacagg
    14401 cggcggcata tcataacctt tgtgtccgac cgttccgaaa ccaagatacc aaacagcata
    14461 tcccgacaga acagattgtg cataaggcac aagcccgcac attccatcaa caaaatgccg
    14521 tctgaacacg ggttcagacg gcatcagtat tttacaatca gaacactgcc tgcaaaatca
    14581 aatagctggc catcgacaat acagcggcgg caggcagggt aatgacccac gccaaaccga
    14641 tgggtttcat cagtttccag ttggcattgc ggttgaccag accgataccg agtaccgcgc
    14701 cgaccaagat atgcgtactg gacacgggca gccccatcag cgacgcgccc atcacgacgg
    14761 atgcggcgga cagttcggcg gtaaaacccg aagcaggatg catttccgcc aaactcgtac
    14821 cgacggtttt aatcacctct ttaccgacaa accacaaacc gacaatcagc gcgatgccga
    14881 aagtcagcat cgcaatcgga gggacggcgc tttgcgcggc aacgctgttc gtccgcaaaa
    14941 catccataat cgcggcaaac gggccgatgg cgttggcgat atcgttcgca ccgtggctga
    15001 atgcgaagcc gcaggcggta aagacctgca tccatgaaaa catctgaaag gtcgatttgc
    15061 ccaagtcttt acgcttgagg cttttggcaa acacaaacgt ccccatccac accgccgcgc
    15121 ctatcataaa gatggtcagg aagctgttga cgttgctcat ccccaaatgc aggtttttca
    15181 agcccttgaa aatcagcata gcggaaatca tcatcgtgcc gaacgaagcg ataaagggaa
    15241 tccaagaatg cagcgccttg taggaatcga cattgttttt acggttgtcg aacgcataaa
    15301 gaccgcggta atactccgat tgcagctctt gaggatcgaa ttcgggttcg tcgtaaattt
    15361 gcgcgtcgtg cgccattttg gtggcgtact cgactttttc ggcttcggac aaaccctcga
    15421 aaaacaggcg gtgccgttct ttataggcct ttttttcctg cttgatgccc ttgagcgttc
    15481 cttccgccca agcgttgtaa tctaagacgt ttttcttgac gcgcgaaaac aggaaatagg
    15541 acaccgcgcc gcccaacacg ggcgacaata cccaagacat cccgatttcg cccagcttgc
    15601 cccaccgtat caacgccacc ccgtccgcat tatgcgtaaa cgccatacac aacgcactgc
    15661 cgacgatgcc gccgataatc gcgtgcgtcg tcgaaaccgg cagacctttg cgggacgcga
    15721 acagcagcca caacgccgcc gccaaaagcg cggacatcat aataaacaca aactgtatgg
    15781 gttcgaaatc aacacccttc aaatcgacga tgcctttgcg tatggtatcg gttacctcgc
    15841 cgcccgcgat gaccgcgccg ctgacctcaa ataccgccgc aatcagcaaa gcctgcggga
    15901 tggtcagcgt gcctgcaccg acgctggtgc cgaaagaatt ggcaacatcg ttgccgccga
    15961 tgttgaacgc cataaacaca ccgaacatcg tggcaataat gaataaggcg gcgtaattgt
    16021 aatgggtata acccaagccc caataaataa aatagccgac tgtacctacc agcatggcgg
    16081 caaagacggc gttgatggtt ttaacatttg cgctcatttg gatttgagcc atggcaatct
    16141 tcctttaatg tggtggaaat catgagtgtt gccgacactg tattgctgcc tccggtcgaa
    16201 ctgccgtttc gggatatggc agacgcatcc ttccttctga accgcatccg aataccccca
    16261 aacctgaccg aatgcaaaac tgctgccgga aacggtttgt atgacacttc tactactgga
    16321 tgttgcgggc gcattataat atttttccac ccgtcttaaa acatttattt acactttatt
    16381 tacactgcgg cggcaaatcg gtatacgagc gtcaatacac gttaaaatgg cgttttgcac
    16441 cagtttggga gtgatgatgg aaacacagct ttacatcggc attatgtcgg gaaccagtat
    16501 ggacggggcg gatgccgtgc tggtacggat ggacggcggc aaatggctgg gcgcggaagg
    16561 gcacgccttt accccctacc ctgaccggtt gcgccgcaaa ttgctggatt tgcaggacac
    16621 aggcacagac gaactgcacc gcagcaggat gttgtcgcaa gaactcagcc gcctgtacgc
    16681 gcaaaccgcc gccgaactgc tgtgcagtca aaacctcgct ccgtgcgaca ttaccgccct
    16741 cggctgccac gggcaaaccg tccgacacgc gccggaacac ggttacagca tacagcttgc
    16801 cgatttgccg ctgctggcgg aactgacgcg gatttttacc gtcggcgact tccgcagccg
    16861 cgaccttgcc gccggcggac aaggtgcgcc gctcgtcccc gcctttcacg aagccctgtt
    16921 ccgcgatgac agggaaacac gcgtggtact gaacatcggc gggattgcca acatcagcgt
    16981 actccccccc ggcgcacccg ccttcggctt cgacacaggg ccgggcaata tgctgatgga
    17041 cgcgtggacg caggcacact ggcagctgcc ttacgacaaa aacggtgcaa aggcggcaca
    17101 aggcaacata ttgccgcaac tgctcggcag gctgctcgcc cacccgtatt tctcacaacc
    17161 ccaccccaaa agcacggggc gcgaactgtt tgccctaaat tggctcgaaa cctaccttga
    17221 cggcggcgaa aaccgatacg acgtattgcg gacgctttcc cgattcaccg cgcaaaccgt
    17281 ttgcgacgcc gtctcacacg cagcggcaga tgcccgtcaa atgtacattt gcggcggcgg
    17341 catccgcaat cctgttttaa tggcggattt ggcagaatgt ttcggcacac gcgtttccct
    17401 gcacagcacc gccgaactga acctcgatcc tcaatgggtg gaggcagccg catttgcgtg
    17461 gttggcggcg tgttggatta accgcattcc cggtagtccg cacaaagcga ccggcgcatc
    17521 caaaccgtgt attctgggcg cgggatatta ttattgagcg caaataccgt ctgaaagcca
    17581 tttccgccgt tcagacggca tttccacccc tttcgtttac aaacccttaa aatccattta
    17641 cactcaaacc cgttcaaaac aaaacaaacc ctgccatgaa aaccctgctc ctcctcatcc
    17701 ccctcgtact caccgcctgc ggcacactga ccggtatacc cgcccacggc ggcggcaaac
    17761 gctttgccgt cgaacaagaa ctcgtcgccg catcgtcccg cgccgccgtc aaagaaatgg
    17821 acttgtccgc cctgaaagga cgcaaagccg ccctttacgt ctccgttatg ggcgaccaag
    17881 gttcgggcaa cataagcggc ggacgctact ccatcgacgc actgatacgc ggcggctacc
    17941 acaacaaccc cgacagcgcc acccgataca gctaccccgc ctatgacact accgccacca
    18001 ccaaatccga cgcgctctcc ggcgtaacca cttccacatc ggtcttaaac gcccccgccg
    18061 ccgccctgac gaaaaacaac ggacgcaaag gcgaacgctc cgccggactg tccgtcaacg
    18121 gcacgggcga ctaccgcaac gaaaccctgc tcgccaaccc ccgcgacgtt tccttcctga
    18181 ccaacctcat ccaaaccgtc ttctacctgc gcggcatcga agtcgtaccg cccgaatacg
    18241 ccgacaccga cgtattcgta accgtcgacg tattcggcac cgtccgcagc cgtaccgaac
    18301 tgcacctcta caacgccgaa acccttaaag cccaaaccaa gctcgaatat ttcgccgtcg
    18361 accgcgacag ccggaaactg ctgattgccc ctaaaaccgc cgcctacgaa tcccaatacc
    18421 aagaacaata cgccctctgg atgggacctt acagcgtcgg caaaaccgtc aaagcctcag
    18481 accgcctgat ggtcgatttc tccgacatca ccccctacgg cgacacaacc gcccaaaacc
    18541 gtcccgactt caaacaaaac aacggtaaaa accccgatgt cggcaacgaa gtcatccgcc
    18601 gccgcaaagg aggataaacc gtgaaaccgc tgcgaagact gacaaacctc cttgccgcct
    18661 gtgccgtagc ggcggccgca ctcatacagc ccgccctcgc ggcggacttg gcgcaagacc
    18721 cgttcattac cgataacacc caacggcagc actacgaacc cggcggcaaa taccacctct
    18781 tcggcgaccc gcgcggcagc gtttccgacc gcaccggcaa aatcaacgtc atccaagact
    18841 atacccacca gatgggcaac ctgcttatcc aacaggcaaa catcaacggc acaatcggct
    18901 accacacccg cttttccgga cacggacacg aagaacacgc ccccttcgac aaccacgccg
    18961 ccgacagcgc aagcgaagaa aaaggcaacg ttgacgacgg ctttaccgtg taccggctca
    19021 actgggaagg acacgaacat catcccgccg atgcctacga cggcccgaag ggcggcaatt
    19081 accccaaacc tacgggagca cgcgacgaat acacctataa cgttaacggc acagcccgca
    19141 gcatcaaact caatccgacc gacacccgcg acgtcagaca acgcatatcc gacaattaca
    19201 gcaacctcgg cagcaatttc tccgaccgcg ccgatgaagc caacagaaaa atgttcgagc
    19261 acaatgccaa gctcgaccgc cggggcaaca gcatggagtt tatcaacggc gtcgccgccg
    19321 gcgcgctcaa cccctttatc agcgcgggcg aagccttggg cataggcgac atactgtacg
    19381 gaacgcgcta tgccatagac aaagccgcga tgcgcaacat cgcccccttg cccgccgagg
    19441 gcaaattcgc cgccatcggc ggcttgggca gcgtggcggg ctttgaaaaa aatacgcgcg
    19501 aagccgttga ccggtggata caggaaaacc ccaatgccgc cgaaaccgtc gaagccctgg
    19561 tcaacgtcct gccgtttgcc aaagtcaaaa acctgacaaa ggcggcaaaa ccggggaagg
    19621 ctgcggttag tggggatttt tctaaatcct acacctgctc cttccacggc agcaccttgg
    19681 tcaaaacggc agacggctac aaagccattg cccatattca agccggagac cgcgtccttt
    19741 ccaaggacga ggcaagcggg aaaacaggat acaaacccgt taccgcccaa tacggtaatc
    19801 cgtatcaaga aaccgtttac attaaagttt cagacggcat cggcaacagc caaaccctga
    19861 tttccaaccg catccatccg ttttattcgg atgggaaatg gattaaagcg gaagatttga
    19921 aagcgggaag ccggctgtta tccgaaagcg gcaaaaccca aaccgtccgc aacaccgttg
    19981 tcaaaccaaa accgctcaaa gcctacaatc tgaccgttgc cgattggcat acctactttg
    20041 tcaaaggcga taaggcggaa acggaagggg tttgggttca taatgattgt ccaaccaaac
    20101 taaaaccgac agaacgttat aatcgtcaaa cgcattatgg aggttctcaa acagatggag
    20161 caagggctca ggcagcaaga caggcaggag aaggtaaacc ttgtccaact tgtggtagaa
    20221 ttcagatatt tggtacgaaa accgcaccaa gtccgcaaca tgaaccccca ttagtaaaac
    20281 attactatga acatggtggt cactctatga gcaatgctga tcgagcgaaa catgccaggg
    20341 aaagcataaa aggaactcaa tgtttaacat gtcaaaggaa agagggtgcg atgatgtcaa
    20401 gatattcgag agaacaggct aaaaagcatg gactataacc aaactgtttt atctcacttg
    20461 caaaaatttt ggaaacatca tgatattaaa ggatttactt ggactttagg aagaattgtt
    20521 gaagaacttc ccgatttcca agttttccaa gtgattccta atcatgaaga tgagccttgg
    20581 gtttatgtca gctctgggat aggtcaattt ttagggcagg agttttttat catcagcccg
    20641 tttgaaacac ctgaacatat tgagaccttg gcaatgctgg catctgcaag tatgcattat
    20701 cctgatcaat ttcaattagg gaaaaccgtc aatatcggta gaccgtgggt cgagcaatca
    20761 tccttccggc attttctgat ttctctgcct tatccttatg ggcaagagtt ggaatatatg
    20821 gataatgtcc gatttttctg gttattgcca attacccaga cagaaagact tttcttgaat
    20881 actcattcgg tagaagaatt ggaaacgaaa tttgatgaag cgggtattga ttatttagat
    20941 ataaatcgtg cgagtactgt ctggcaggca ggataaaaat tcggcaatca aaatgccgtc
    21001 tgaaacaagt ttggactttc agacggtatt ctgatgaatg gaacaggata ctgggaactg
    21061 agttcttcaa aaatcctatc tccgtccctt atacgtcggt gctttagtca aaacggcaga
    21121 cggatgaaaa attattgccc gtatccttgc caaagatgaa gcaagatgaa gccaggtaat
    21181 tgaatcggta caagcgcaat gtccgacttg ttctgcaaaa cagggaggga gattaagcca
    21241 atattcaaaa gaacagaaaa agaaactgag taatgggaat aaaaaatgaa caaataataa
    21301 tttgtaaaaa atataataca gaaatatatc ctgtttccga tgtttcaaaa atcggtgttg
    21361 ctgaaaatgt caaacagaca ggcctgtatc cgattaacgg gttaaggcac cgtcccaaag
    21421 gtgatactaa cggttggtat atctgggccg gtgaaaactt ctcctatgat aaaaattttt
    21481 tcctgccctt gcatacgttc catttgcaaa tatggcgacc cgaaatcatc ccctttttga
    21541 cacttccgcc cggttaccgt tttttaattg gggaaaacgg ttatgaagac gtatggtttg
    21601 atgagttgct attaaatgat aattgacgaa aaatctactt cagatgtact gaaacaatat
    21661 caagaactag ggtttgattt gacaaaacct atgataatag agttttttat agatggttct
    21721 caaaagaatc tgcaatcaat agaaagccaa ctcatttctt atcggcaaga ttttcaaatc
    21781 agtattgaac aagatgaatt cggagaaatg tggacttgct attgttccgt caatatcgtc
    21841 ccttcccttg aaaacatttt agctattgaa actacgttat ttggtattgc acaaaaaaat
    21901 gattgtaatt atgaaggttt tggttcgtat gggaactaac aaaaagtgta ggttgggttg
    21961 aaaaaaccat attcccatta aagattaggg gatgtctatg gaaataaaat tcaatacttt
    22021 gggggttatc ctgaatggag tcaatcctga agaaaaattc ataaaaatta ttgatgacca
    22081 ggaaaatacc ggtggatttc tgatactgtt atcttcaaat gacaaattct catcatttga
    22141 cagttacgat gactgggtcg aaaacttaga aatattaaaa gaatacctgc aagaatctca
    22201 ttggataata aagtgggtcg gatagtcggc aaacgtagtc cgtttgaaac gcttttactt
    22261 ttcagacgga ctttctacca atgggaacaa ggcgttttgt ctcaatccgg tttatgatta
    22321 ttcgatacag tgatgggaag ctaagaaaaa tatgattaat catattgaaa aatatatagg
    22381 cactatttct catggctcga aaagcgattc gggaagtcag tacaaattaa atatcgcagc
    22441 tattccctct tcccctaaca gggatttaaa aacttatatt actttagggt taagcaaaca
    22501 tgatctgaat tataaaagca gatttgaaat actttttgta tgttctttaa aatatgatga
    22561 aaatcagatt ttcccatttt taagatggtt agcagaaacc atcattgaga ataaaaaaaa
    22621 tccttcttcg ggggcaggtt gtttatttgc ctagaagcat tgttaattca acaaaaatgg
    22681 acgcactgta tgtttccgcc ccattttatt tcgacgatga tttccaagtc tgttatggcg
    22741 aacactacaa tattgttttc cctttgcttg tccccttgta taaacaggaa gccgaattgg
    22801 tggaaaaaaa gggttggaat gcttttgagc agttcttgtt ggataatgaa gttggcaacc
    22861 tttcggatat gaataggaaa ccgtttgttt ggtaagggct ttcttaaatt tgattaaagg
    22921 tcgtctgaaa ttaagcttgt ttttcagaca acctttcctt catttgaaac agaatattga
    22981 aaactaagtt cttcaaaaat cctacacctg ctccttccac ggcagcacct tggtcaaaac
    23041 ggcagacggc tacaaagcca tcgcccatat tcaagccgga gaccgcgtcc tttccaagga
    23101 cgaggcaagc ggagaaacgg gatacaaacc cgttaccgcc cgatacggta atccgtatcg
    23161 ggaaaccgtt tacattaaag tttcagacgg catcggtaac agccaaaccc tgatttccaa
    23221 ccgcatccat ccgttttatt cggacggcaa atggattaaa gcggaagatt taaaggcggg
    23281 aagcagactg ttatccgaaa gcggcagaac ccaaaccgtc cgcaacaccg ttgtcaaacc
    23341 aaaaccgctc aaagcctaca atctgaccgt tgccgactgg catacttact tcgtcaaggg
    23401 caatcgggcg gaaacggaag gggtttgggt tcataatgag tgtccgtatg gaaaaggcaa
    23461 ccaacgatat aaagatgctc cttatcatgg taaaaatgat aattctgtga aaagtagagc
    23521 gccaacaaat gggcaagctg tccttgataa ctctgttcaa gttaaatcga catcttctca
    23581 aagagttgga gttgataaaa ccaataatga aatcgttgta ttaaaccaaa ctagaatttt
    23641 taatgacggt tctgctgagt atcatgggca tgtaaggaac tggaaaaatt tacatactga
    23701 tcaacaaaat gctttaaaga aagcaggatt ggttaattca aaaggaaaaa tcaaaaaatg
    23761 atggctgata aggaacaagt agaaaagatg atttattctg aaaataaaga ttgtgttatt
    23821 gatggtatgc tcggtatgac cttcagctcg gaggaggatg aaatcccttg gatttccgag
    23881 aaattgacgg agttgtcgaa gcataaagac ttggatattg ccagattgtc attaacctgt
    23941 ttcggacatc tcgccaggat gcatgaaaat atcggcgatt gcgataaagt gattgcttta
    24001 ttgcgctcaa aacaaggtaa tccggatttt caaggttttg ccgaagatgc gttagacgat
    24061 atttccttct ttatatttaa aaagcgtccg taagcgcgga ttaaacccgg aaatccgatg
    24121 cgtctgaaag tacattccgg ctttcagacg gcatcggtaa cagccaaacc ctgatttcca
    24181 accgcatcca tccgttttat tcggacggca aatggattaa agcggaagat ttgaaagcgg
    24241 gaagcaggct gtttgctgaa aacggtgcag agcagaccgt tcaaagcgtt accgtcaaac
    24301 cggaaccgct caaagcctac aatctgaccg tcgccgactg gcatacttat ttcgtcaagg
    24361 gtagtcaggc ggaaacggaa ggggtttggg ttcataatga ttgtccgcct aaaccaaaac
    24421 caaccaatca tgcccaacaa agaaaagaag aagctaaaaa cgattctcat cgaagtgtgg
    24481 gagattccaa tcgtgtcgtt cgcgaaggaa agcaatattt agattccgac acaggaaacc
    24541 atgtttatgt aaaaggagat aaagtggtta ttctaactcc tgatggaaga caggtaactc
    24601 aatttaagaa ctcgaaagcc aatacgtcaa aaagggtaaa aaatgggaaa tggacaccaa
    24661 aataatcaat cttccttaga aaaaagaatt ttttatctgg agcattcggg gcagtatctg
    24721 atgatttgcg ccctatcgga ttactcccaa aataaacata ctgtcgttat ggcgaatttc
    24781 ctctatccaa atgaaaaaat ggattggaga aatttagatg atttgttcaa tgagttggtt
    24841 ttagaggaat tacagtcttc atttatggat tggtatccaa ctattgaaaa ggcaattagc
    24901 catcatttag aagacttttc gtaacaagca taaatcaaaa agtcgtctga aactgctttt
    24961 aattttcaga cgacctttcc ccatatcggc ggattatgaa tttacagcta tcgggaaatt
    25021 gagttatctc tctgatagag aaaattccat acgcgaagcc gttgaccggt ggatacagga
    25081 aaatcccaat gccgccgaaa ccgtcgaagc cgtcttcaac gttgccgcag cagccaaagt
    25141 cgcgaagttg gcaaaggcgg caaaaccagg gaaggctgcg gttagcgggg attttgctga
    25201 ttcttataaa aagaaattgg ctttgtctga tagtgcgaga cagttatatc aaaatgcaaa
    25261 gtatagagaa gctctagata tacattatga agatttaatt agaagaaaaa ccgatggtac
    25321 atcaaaactt gttaacggta gagaaattga cgctgttacg aatgatgctt taatacaagc
    25381 caaaagaaca atttcagcaa tagataaacc taaaaatttc ttaaatcaaa aaaatagaaa
    25441 gcaaattaaa gcaaccatcg aagcagcaaa ccaacaggga aaacgtgcag aattttggtt
    25501 taaat
//
LOCUS       DAXFZR010000020        24629 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000020 DAXFZR010000000
VERSION     DAXFZR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24629
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.020"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(436..521)
                     /locus_tag="ACFJAS_000723"
     tRNA            complement(436..521)
                     /locus_tag="ACFJAS_000723"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(485..487),aa:Leu,seq:gag)
     gene            complement(535..885)
                     /gene="secG"
                     /locus_tag="ACFJAS_000724"
     CDS             complement(535..885)
                     /gene="secG"
                     /locus_tag="ACFJAS_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC3982026.1"
                     /translation="MEAFKTLIWIINIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNIRQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(892..1665)
                     /gene="tpiA"
                     /locus_tag="ACFJAS_000725"
     CDS             complement(892..1665)
                     /gene="tpiA"
                     /locus_tag="ACFJAS_000725"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC3982027.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEERETGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            1848..2351
                     /locus_tag="ACFJAS_000726"
     CDS             1848..2351
                     /locus_tag="ACFJAS_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC3982028.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADEADWVANLANTAAVLK
                     EAFGWLWVGFYFVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGETVVVKDVNA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDEADALYLGELAKILERRF
                     EASSQAA"
     gene            2454..3110
                     /locus_tag="ACFJAS_000727"
     CDS             2454..3110
                     /locus_tag="ACFJAS_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC3982029.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFADESLQG
                     LAYADMELPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            3191..3514
                     /locus_tag="ACFJAS_000728"
     CDS             3191..3514
                     /locus_tag="ACFJAS_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC3982030.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(3777..5954)
                     /gene="tdfF"
                     /locus_tag="ACFJAS_000729"
     CDS             complement(3777..5954)
                     /gene="tdfF"
                     /locus_tag="ACFJAS_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
                     /protein_id="HFC3982031.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPFGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     PDGTGEPSATVNLVRKHPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQLERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWSNSRNRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHSTAATDLIPGYWHADPRTHSASMSLTGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYDTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRAGSYNSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQLQ
                     KDEHGSYLKPVTGNNLEADIKGEWLEGRLNASAAVYRARKNNLATAAGRDQSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKPRDQDGSRPNPDSVPERSFKLFTAYH
                     LAPEAPSGRTIGAGVRRQGETHTDPAALRIPNPAAKARAVANSRQKAYAVADIMARYR
                     FNPRTELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
     gene            complement(6217..6540)
                     /locus_tag="ACFJAS_000730"
     CDS             complement(6217..6540)
                     /locus_tag="ACFJAS_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC3982032.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
     gene            complement(6573..7544)
                     /locus_tag="ACFJAS_000731"
     CDS             complement(6573..7544)
                     /locus_tag="ACFJAS_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3982033.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            7801..8757
                     /locus_tag="ACFJAS_000732"
     CDS             7801..8757
                     /locus_tag="ACFJAS_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3982034.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFDLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            complement(8870..10885)
                     /locus_tag="ACFJAS_000733"
     CDS             complement(8870..10885)
                     /locus_tag="ACFJAS_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC3982035.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(10981..12291)
                     /gene="argA"
                     /locus_tag="ACFJAS_000734"
     CDS             complement(10981..12291)
                     /gene="argA"
                     /locus_tag="ACFJAS_000734"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC3982036.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            complement(12288..12716)
                     /locus_tag="ACFJAS_000735"
     CDS             complement(12288..12716)
                     /locus_tag="ACFJAS_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC3982037.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            complement(12790..13431)
                     /gene="pyrE"
                     /locus_tag="ACFJAS_000736"
     CDS             complement(12790..13431)
                     /gene="pyrE"
                     /locus_tag="ACFJAS_000736"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC3982038.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            complement(13493..14230)
                     /locus_tag="ACFJAS_000737"
     CDS             complement(13493..14230)
                     /locus_tag="ACFJAS_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC3982039.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARRQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            complement(14224..14664)
                     /gene="rimI"
                     /locus_tag="ACFJAS_000738"
     CDS             complement(14224..14664)
                     /gene="rimI"
                     /locus_tag="ACFJAS_000738"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC3982040.1"
                     /translation="MNIRPALPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCTADGQTEDAVLMEKIC"
     gene            complement(14661..15338)
                     /gene="tsaB"
                     /locus_tag="ACFJAS_000739"
     CDS             complement(14661..15338)
                     /gene="tsaB"
                     /locus_tag="ACFJAS_000739"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC3982041.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGGYPATGAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            complement(15477..16334)
                     /locus_tag="ACFJAS_000740"
     CDS             complement(15477..16334)
                     /locus_tag="ACFJAS_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC3982042.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGDRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            complement(16473..17537)
                     /gene="fba"
                     /locus_tag="ACFJAS_000741"
     CDS             complement(16473..17537)
                     /gene="fba"
                     /locus_tag="ACFJAS_000741"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC3982043.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            17731..18648
                     /gene="xerC"
                     /locus_tag="ACFJAS_000742"
     CDS             17731..18648
                     /gene="xerC"
                     /locus_tag="ACFJAS_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC3982044.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            18733..20646
                     /gene="dxs"
                     /locus_tag="ACFJAS_000743"
     CDS             18733..20646
                     /gene="dxs"
                     /locus_tag="ACFJAS_000743"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC3982045.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVATALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(20711..20875)
                     /locus_tag="ACFJAS_000744"
     CDS             complement(20711..20875)
                     /locus_tag="ACFJAS_000744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982046.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(20893..22221)
                     /gene="miaB"
                     /locus_tag="ACFJAS_000745"
     CDS             complement(20893..22221)
                     /gene="miaB"
                     /locus_tag="ACFJAS_000745"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC3982047.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLAEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            22424..22504
                     /locus_tag="ACFJAS_000746"
                     /pseudo
     CDS             22424..22504
                     /locus_tag="ACFJAS_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            22558..22941
                     /locus_tag="ACFJAS_000747"
     CDS             22558..22941
                     /locus_tag="ACFJAS_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC3982048.1"
                     /translation="MYCLRLRRLVLIFVNPLYQTEEGIAQTVVKGKTTRAEVEARFGK
                     RNPFGCYAYHEVSLPIYNFLPTNFIYMKSERRHWEWCADYDGEGVVRDYRFTHKVEKD
                     ERSVIRDTVGVIRKEAGKSLSQPEK"
     gene            23005..24285
                     /gene="hemL"
                     /locus_tag="ACFJAS_000748"
     CDS             23005..24285
                     /gene="hemL"
                     /locus_tag="ACFJAS_000748"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC3982049.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(24509..>24629)
                     /locus_tag="ACFJAS_000749"
     CDS             complement(24509..>24629)
                     /locus_tag="ACFJAS_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC3982050.1"
                     /translation="SPEPNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         4768 a         6443 c         7682 g         5736 t
ORIGIN      
        1 gccgccttgt cccgatttaa atttaattca ctatatttca tatacttatt tatctaactt
       61 ttccccggta acaaggtaac aactaagggt atcgcttcct agtataggtt ttttccaaaa
      121 acccaaaaaa accgctctgc atcttggacg gtttccccct cgttccctac ggttcgtcag
      181 tattccccgc aagctctttt ccgcttggca ttcctgattt ggtggtcgtt cagctttatt
      241 tttgcggtat ttttcggata ccgtaaaaaa tcaggttagt tcaattaggg cggattggac
      301 gggattgcac agtcaggaag gggacggcag gataacaatc agcctgaaat ccttgtattg
      361 attgggattg gttgatggca tcagacagga ttaaacgaaa aaaaacgcct agaatttcta
      421 agcgtttttg tgtgttggtg ccgacagcga gatttgaact cgcacagcct acggccacta
      481 ccccctcaag atagcgtgtc taccaatttc accatgtcgg catttgaaaa actgttattt
      541 ctgctgctga ggaacagggg cagaaggttc ggtattgctt acgggtttgg gtgcttgctg
      601 agtctgtcgt atgttgctga agtccaaacc gtgttttgtc gtgtgggtgt gaatatacac
      661 catagccatg caggttgcga agaaaaatgt tgctgcaacg gcggtcgagc ggctgaggaa
      721 gttggcgttg ccggcagagc cgaatacgcc ttgcgcgctg ccgcttcccg atccgaaggt
      781 cgcgccggca tccgcgcctt tgccgtgttg gagcaatact aacacgatga cggccaaagc
      841 ggaaataata ttaataatcc aaattagggt tttgaaggct tccatatttt tctacgcatt
      901 ttgtgcggca ctgatgatgg cggtaaagga gtcgtacgac aatgacgcgc cgccgacgag
      961 tgcgccgtcc acataaggta ctgcgaagat gtcggccgcg ttgtccgctt tcacacttcc
     1021 gccgtaaagg acgcggattt taacatcgct tccgcacaaa gacaagattt ctttgtagat
     1081 gaatgcgtgc atatcggcaa tctgttcgac ggtggcgact ttgcctgtgc cgattgccca
     1141 gacgggttcg taggcgacgg cgatgttttt ggtatccagc ccttgcagga tggaaagctg
     1201 atgggcgatg acttcgtgtt ctttgccggt ttcgcgctct tcgaggcttt cgccgacgca
     1261 caataatggg atgagtccga cgttgaggac gttttccatt ttgcggcgtt ggatttcgtt
     1321 tttttcgccg aaataaaggc tgcgttcgga gtgtccgatg aggacgatgt ctgtgccggt
     1381 gtcggcgagc atttcggcgg acacttcgcc ggtgtacgcg ccgttgtcgg ggaagcggct
     1441 cacgtcttgg gcgcaggtga ggatgcggtt gtttaagacg atttgcatgg cgttgtgcag
     1501 ttgcagcagg taaacggtcg gggcggcgag tccgatgagg acgcgttcgg cggtggggag
     1561 gatgcggaag cggtgcatca gtgcgttgtt gttttggagc cggccgttca ttttccagtt
     1621 gccgatgacc catttttgat cccacattcc gatttggcga tacatctttt ttgctccgtg
     1681 tcgtgttttt ctgtctgccg cgtgtggcgc gttgcaatgt gaagtttagt ggatatgcgg
     1741 cgggttcgca acttggggcg ggcggctgcg ggggcggttt ggaatgttgt ttcgggcagg
     1801 atgttttata atggctgcct gatatgtatg caaatatggg agatatgatg cacgcgcttc
     1861 atttttcggc ttcggacaag gccgcgcttt atcgtgaggt gttgccgcag attgagtctg
     1921 tggtggcgga cgaggcggat tgggtggcga atttggcgaa cacggcggcg gttttgaagg
     1981 aggcgttcgg ctggttgtgg gtgggttttt attttgtcga tacgcgttcg gacgaattgg
     2041 ttttggcacc gtttcaggga cctttggcgt gtacgcggat tccgttcggg cgcggggtgt
     2101 gcggtcaggc ttgggcgaag ggtgaaacgg tggttgttaa ggatgtgaac gcgcatcccg
     2161 accatattgc ctgttcgtct ttgtcgcgtt cggagattgt cgtgccgctg ttttcagacg
     2221 gccgctgtat cggcgtgttg gacgcggaca gcgaacattt ggcgcagttt gatgaggcag
     2281 atgctttgta tttgggcgaa ctggcgaaga ttttggagag gcggtttgag gcttcgagtc
     2341 aggcggcttg agactggcaa aacgggcggg cttcgcgtgc cgaagttggc gcggcggtag
     2401 tgtggtttta taatgcctgc cattgataaa acaattattt gacggagcac taaatggatt
     2461 ttgaaaaagc gcggttcaat atggtcgaac agcagatccg tccttgggat gtattggatt
     2521 ttgacgtgtt ggacgctttg gaggagattc cgcgcgagct tttcgcggat gagtctttgc
     2581 agggtttggc gtatgcggat atggagctgc cgcttgccaa cggtcataag atgctcgagc
     2641 cgaaagtcgt ggcgcggctg gcgcagggct tgaagctgac gaaaaacgat acggttttgg
     2701 aaatcggcac gggttcgggc tatgcgacgg ctctgttggc aaaactggct ggccgtgtgg
     2761 tttcggacga catcgatgcc gaacggcaaa agcgcgccaa agcggttttg gacggcttgt
     2821 ctttggaaaa catcgattat gtgcaaaata acgggttgac cgaactttcc gcaggtgcgc
     2881 cgtttgatgc ggtttacgtc ggcggcgcgg taacccttgt gcctgaagtg ttaaaggaac
     2941 agttgaagga cggcgggcgt atggcggtta ttgtgggacg caggccggtg cagcgcgcgc
     3001 ttttgattac gcgcaggggc gatgtgtttg aagagaaggt gctgttcgat actttggtgg
     3061 cgcatttgga cgacaaggat gcccatcctt tcgacagttt taatttttga tgttcggatt
     3121 gtgatgccgt ctgaaagcgg gtttggggct tcagacggca ttttgcttgg tttttttcgg
     3181 ggggtttgtg atggatattg tgcaacttcc gtcggcggca ttgaaggcgt ggatggacga
     3241 agggcggatg ttttgtttgt tggacgtgcg tacggatgaa gaagcggcgg tttgttcgct
     3301 gccaaatgcg ctgcatatcc cgatgaatct gattccgctg cggcaaaacg agttgccgga
     3361 cgatgtgccg cttgtggtgt attgccatca cggtatccgc agcctgcata cggcgatgta
     3421 tttggcggag gcgggttttg aaaacctgta caaccttcag ggcggcatcg acgcgtgggc
     3481 ggttgaggtt gacgcggaaa tggcgcggta ttgaaggcgg cttcagacgg cattccttaa
     3541 atgcgtgtac ctctggtgtt ccataaaggt cgtctgaaag tgcagcttct gcgaagctaa
     3601 agcgcagtat caacgaagtt aaaatttgcc tgaaccttaa aggcagcctg caccccaatt
     3661 ccctcgcccc gtgggagaag gctagggaga gggcggcaaa ctgcaggttt gcttgggcgg
     3721 cattttcaat gtgcaggctg cttttagctt cgcagaaact ccgttttcag acgaccttat
     3781 ttaaaccgat aggtaaacgc cgcgttcact gtccgcagtg cgccgtagct gtggcggtcg
     3841 ggctgggtgc ggtagtgttt gttgaacagg ttgtccacgt tcagcgacag ttcggtgcgc
     3901 ggattgaagc ggtaacgcgc catgatgtcg gcgacggcgt aggctttctg gcggctgttg
     3961 gcgacggcgc gggctttggc ggcggggttg gggatgcgga gcgcggctgg gtcggtgtgg
     4021 gtttcgccct gccggcgcac acccgcaccg atggtccggc cgctgggggc ttcgggggct
     4081 aagtggtagg cggtgaagag tttgaagctg cgttcgggta cgctgtcggg gttcgggcgg
     4141 ctgccgtctt ggtcgcgggg tttgctttgg ctgtagcctg cctgtatctg ccattcgggc
     4201 gtgatgcggc cgccgacttc gatttcccag ccgtgggttt tggcttggtt ggcggcgcga
     4261 tagtaggtgt tgccgctctg gtcgcgtcct gctgcggtgg cgaggttgtt tttacgggcg
     4321 cggtacacgg cggcggatgc gttcagacgc ccttcaagcc attcgccttt gatgtcggct
     4381 tccagattgt tgccggttac gggtttcagg tagctgccgt gttcgtcttt ttgcaattgc
     4441 gggacgaaca ggctgctgta cgagccgtaa agcgacaggt tgccggtcag atcgaacacg
     4501 atgcctgtgt agggggtgaa acggttggcg gacacatagg tcatgccttg tgtgcggctg
     4561 ttgtagctgc ctgcgcggta gcggctgtat ctgccgccga gtatcagcga aaggttgtcg
     4621 gcggcgcgga aacgggtggc gagatagccg ccgatttgcc gcctggtgtc gtattgcggg
     4681 atggtttggg caaacgatga tggctgcgga taggcgcccg tgcgggaaaa ttcgtaggcg
     4741 ttgggaatgg cgttgggaat gatgctgcgt tcgccgtatt tgttgctggc gtatttgtag
     4801 ccgttgatac ccgcgattaa atcgtgctcg cggccgaaca ggcggtattt gccggtcaat
     4861 gacatgctgg cgctgtgggt gcgcggatcg gcgtgccaat aaccgggaat caggtcggtg
     4921 gcggcagtgc tgtggtcgat ggaaagtacg cctgccacac cgtagggctg gcggaagcgg
     4981 ctacgggtgt agtcgtattc ggctttgagt ttccagtctt ggttgaagcg gtgttctatg
     5041 ccggcgaaca ggttgagcgc acggttgcgg ctgttcgacc aatttgtggc ggggttgtct
     5101 ttcgggccga aggcggtggc ataaccttgg ctgtcgtaca cggcgtagct gagcggcgcg
     5161 tctgcggttt ctttcgcctg ctggtagtcc atgcctgcgt ggacgcgggt ttgcggtgcg
     5221 atgtcgtatt ccaaaatgcc gtagagttcg gcatcgcggc tgcgttcgag ctgccgccac
     5281 gagtcgccgc gtccgaaggt ggaaaccagg cggccgcgca gcgtgccttc ggcgttcagg
     5341 ctgcccgata cgtccgcgcc cagcccgaaa tgtttgcggt tgccggcttc ggcgcggact
     5401 tcaaacaatg gcttgcgggt cgggtgtttg cgtaccagat tgacggtggc agaaggctcg
     5461 cccgtgccgt ccggcagccc cgccacgccg cgcacgactt ccacgcgctc ataggcggcg
     5521 gtgttggcat tgcccgtatc ggccagcgcg tcggcaacgg ggatgccgtt gatttggtag
     5581 ttggcgatgc ggctgccgcg cgcgaacagg tagttgtagc ccgcgcggtc ggagccgtaa
     5641 atctggcggc tggtgccggt cgcctgcaac agggcgcggt cgagcgtttt gatgttttgg
     5701 tcgcgcattt gttgcgatgt gatgacgctg acgctctgcg ggatttcgcg cagggtcatg
     5761 ggcagcccga acggggtgtg cgtgccggaa acggtgtagc cgtcgttgga actcgcggtg
     5821 cggtcggcgg taacggtgat ggtcggcaat tcggtgcttt cctgcggttt ggggtcgtct
     5881 gaaacagaaa catcggcctg cgcgtacacg ggtagcaggg cggcaaaaag cagggagtat
     5941 ttgaagcgtg tcatggggtt tcctttgtgt gttggtgtgc ggtggggttg tatagcggat
     6001 taactttaaa ccggtacggc gttgccccgc cccggctcaa agggaacggt tccctaaggc
     6061 gcccaagcac cgggcgaacc ggttccgtac cattcgtact gcctgcggcc cgccgccttg
     6121 tcctgatttt tgttaatcct ctatgctttt ggcatattga aggtgtaggc tgcttttagc
     6181 ttcgcagaaa ctcagcttcc ttcggaaact tcgttttcag gcggcctcgg acgggtcggg
     6241 cagatcaatc agcaccaccg agcagccgtt ttccgaacgc gggcggtgcg acaccgactt
     6301 cggcacgacc gccatctcgc cctcgcgtat cgtcatgttg ccgccgtcgg cgaagtccac
     6361 cgccatgtcg ccctccacgg caaacagcac tttgtcgctg tatccgtgcg tgtgccagcc
     6421 gtaatcgccc aaaagtttga ccaggcggaa ttggaacccg tggcggttga tgatttcggg
     6481 ctgccagtat tctttgatgg cggcaaggtg ctgtttcagg ttgatggttt cgttttgcat
     6541 ggggttctcc ttgtggtttg ttttgaataa aactactgcg ccgcaacggg ttctgccttt
     6601 tcaaacgccg ccttcaactg ttccgccgcc tgtatcaact gccgcgcgcc gcccgcgaca
     6661 atgtagttcg cggcaggcat gacgatgatt tgcttgcgct tccaagcgtt cgtgccgcat
     6721 accagcgcgt tatccaacac ttccacggca gccggccctt cctgcccgat ggcggcggtg
     6781 cggtcgatga tgaaaatcca gccggggttt ttctctttga tgtattcgaa ggaaacgggc
     6841 tgcccgtgcc cttcgttgcg taaagattcg tccacgggcg gcaggccgat gtcgccgtgt
     6901 atccaacttg ccaaccgcga ttgcgtgccg aaggcggaca ccttgttgcc tgtaaccgac
     6961 agcaccagcc cgcgtccttt gcctttggcg gcttcgcgct tttgggcgaa cagcgcgtca
     7021 atctgcgcat tcaattccgc cacgcgcgct tccttaccga aaatccgcga cagggtctcc
     7081 atctgcttct cgccgctggt gcggatattg ccgttgtcca ccgtcaaatc tatggtggtc
     7141 gcgtttttcg ccaactgttc atacgcttcc gcacccggcc cgccggtaat gacaaactgc
     7201 ggattgtggc ggtgcaggga ttcgcaatcg ggctcaaaca gcgtccccac cgttgccgcc
     7261 ttgtcaaatg caggctgcaa atagtccacg cgcaccggcg cggtggttgc gcccacattc
     7321 acgcccggct cggtcagcgt atccaacgcc gcccagtcgt acacggcgac gcgttcggga
     7381 ttcttcggca caacggcatc gccccgcgcg gtcggcacgg tcagcgtggc gacaggtgtg
     7441 gatgcggctt gggatgcggc ggatacagtt ttttcggcgg caggttcggg cgaacaggcg
     7501 gccggcagga cggcgcaggc tgcccaataa aaacgcggtt tcacggtgtg cggttccttt
     7561 gcatacgaat aagaatgttt attattaagc aaacaacctg cagaaaccac gccgcaaaac
     7621 gcaaaattcc cgatacggca aaacaaattc ccgatacggc aaaacaaatt cccgaaacgg
     7681 aaaaggtcgt ctgaaacacc ctttcagacg accttttccg ttccaaccaa tccctgtgct
     7741 tgattattgg taataattcc tatttaattc atttgttaga caactcgttc ctatccaatc
     7801 atgaacaccg ccgccatcta ccgccagtac caaacctatg tccgctccga taaatccggc
     7861 tgggcgttgg acggctgttc cgacagcgca ctcattgcgc aggcaaaaca gcccggtttg
     7921 catctgaaaa tgtgcatcaa ccgcttcgac tcgggcatca ccttatcacg gatgcgcggc
     7981 ggcggaacgg gtgcgtttcc caccgaaatc cacaatttca gccacaactg cgccttgttc
     8041 gtcatggtat cggggcagaa ccggttacaa atgggcggca gggaataccg cccgtctgcc
     8101 ggcgaaatct ggctggtacg cggcgaactg gcggacgtat ccgaaaccct gctgccagac
     8161 agcggcggca tgtgcgcgct gcatttggat ttttcgctgg aaaaactgcg ccgctggcac
     8221 gacgaaggtt tactggacga acgcctgttt tcgccgcaaa cgataggccg attcgccctg
     8281 caacggctgg cgcaaaacgc ggggacgctg acggcggcgg cctgccccct gctgcaacgc
     8341 ccgttcgaat cggacggctt cgacctgctc gccgacgaag ccgacgcgct ggaactctcc
     8401 gcccgattat tacgcttcac cttccgccgc cacgacaacg gctaccgccg ccggcgcata
     8461 gacgaagccg ccgatatttt gaacagcgaa ttcgcccgcc cgctgaccat cgccgaaatc
     8521 gcccgccgcg tcgggctgaa cgaatgctac ctgaaacgct atttcaaagc gcaaaccggc
     8581 gaaaccgtcg ccggacgcct gcgccgcctg cggctggaac acgcgctcgc cctcatcgaa
     8641 tcgggcagca ccatccaagc cgcgatgcac ttttgcggct accgccacgc gggacggttc
     8701 aacgaagcgt tcaggcggca ttacggattt ttgccttcgg atgtgaaaaa gtgctgatgg
     8761 gccgacggca tagatatttt gatttgaaac aaccattaaa aaagatcgtc tgaaaaagca
     8821 atttcagacg atcttttttc attcgcggcg gcagtgtcgc cccctgccct tatcccaaaa
     8881 attctttcaa aaacagcaac acgcaggcga gtttgtcggc ggattcgcgt tgggtgccgt
     8941 tgccggtatg gccgccgccg tcaggcgagt agagccaaga ttgcggcgag gtttcgcgca
     9001 gtttggcgta gaatttgagc gcgtgggcgg gatggacgcg gtcgtcgctg aggctggtgg
     9061 taatgagtgc gggcggataa tcgatgccgt ctgaaagatt gtgatacggc gacaattcgc
     9121 ccagccggcg tttgcaggct tcgtatttct gcggattgcc gtattcgtcc gtccaacttg
     9181 aaccggcgga cagcagcgga taacggatca tatcggtcag cggtacttcg cacaccagcg
     9241 caccgatgct ttgcggttcg cgcacgaagg cggcggcggt aatcaggccg ccgttgctgc
     9301 cgccctgcaa gccgatgtgt ttgggcgaac tcatgccgcg ttcggacaaa tcacgcacga
     9361 ctgccaacaa atcatcaacg cttttgtgtt tgctgattcc ctgcgccgcc tgatgccagc
     9421 gcgggccgaa ttctccgccg ccgcggatgt ttgccaatac aaaggcattg ccctcttcca
     9481 gccaatattt gccgacgctg cccagataat gcggcaattc aggaatgccg aaacctccgt
     9541 aagcatagac taaggtcggc gtgtcgggcg cggcgttttt gccgacgtgg aaataaggaa
     9601 tgcgttcgcc gtcggacgac accgcccaaa actgccgcac ttcgatgccg tctgaaacaa
     9661 actgctgcgg ctggaggcgc atgacggtca gttccatcac gttcaaatcc agcgcaaaca
     9721 gcgtcagcgg cgtggtgaaa tcgctggcgg caagataaac cacgtcgccg ccccacggtt
     9781 ggtcggtcat ttccaacgcg cccgagggca ggtgcggcaa ctcggcttcc tgccatttgc
     9841 tgtcggcaaa ccgccacgct ttcagacggc cttgtacatt ctccagcagg cttgccacca
     9901 caaaacgctt ggtcgtttcc acgctttcca atgcctgcgt ttcatcgggc gcaaacaaaa
     9961 gctgcgccgc cccgagttcg ccccgattca gtttcaccgc caccaacgcg ccactcggat
    10021 agctttggtt cgcgcggtgc cagtccttgc gcagcgtcag caaaagatgt cccgccagat
    10081 agccgaccac atcgcaatca ttaggcaggt tcaacggttt cgccccgcct tcggacgaca
    10141 cctgcaaata cgtcttggtg taaaaaccgt ccgacgcttc aatcaaatca atcggcgaac
    10201 cctgcggatc gaggtaacgc cacgcgttta ccatcatcgc gcctttatcg atttggtacg
    10261 ccggcaggct ttcctcgaaa ctcttgccgc gttccaccag ccacacttcg cgcggatagc
    10321 ccgattcggt caactggcgt tcgtcccaag ccggacacac ccacacgctg ttttcatcgc
    10381 gccacgacac atggtttttg cctgccggaa agtgaaaacc gccctctacc aattcccctg
    10441 cttccaaatc cacttccagc gtatacgccg tatcgccgcc cgatttgttc aaagtcagca
    10501 gcgcgcggtt gggctgctcc accaagtgcg acacgccgcc caaatacaca tcgtcgccga
    10561 gcaactcatc gaaatccgcc accgaaaaca ggattttcca ctcgggatag ccggaacggt
    10621 aggtcgccgc cgtacacacg cggtacacgc ccttcggata ttccgcattc tgatggaaat
    10681 ggtacatccg cgcgcggtgt tcctgacaaa acggaatctg ccgcgtgtcc tgcatttgat
    10741 tcaaaatgcc gtctgaaagt gcgcgcgcct tgtcgttgtt taaaaaacgc gcgcgcgttt
    10801 cggcattcgc ttcagcagcg aagttttgcg tttcggcgga atcgaggttt tcaaaatggc
    10861 ggtaggggtc ggggtaggat ttcatcgggg tccttgaggg tcgggcaggt ctttataatc
    10921 ggggaaatgc cgtctgaaac ggggttcagg cggcatttct gcggcggctt tccgttgcgg
    10981 tcagcggtgc aggcgacgca ccagaatatg cgagtttcgt ccgttgctgc ggtagtcttt
    11041 gcgccgcgtt tcgggcagct cgtcttccga tgccgtctga aagccgcgtt cggcaaacca
    11101 ttcgccggta tttgtggaca gtgcgaacag cctgcttatg cctatgccgc gcgccttatc
    11161 gataatgtgg gcaagcaggc gttcgccgta gccgccgtcc tgtgcctgcg gcgagacggc
    11221 aaggcaggcg atttcgccgc aatcggcttc ggcaaaggtt ttgagtgcgg cacagccgta
    11281 caggttgccg tcgtgttcga ggatggaaaa ttcggaaatg tggttttcga ggtattcgcg
    11341 gctgcggtgc aataggatgc cctgttcttc cagcgggcgg atgagggcgg cgatgtgcgg
    11401 gatgtcgccg ctgtgcgcct gccggatgga gacgaaggct tctttggcaa tggacgtgcc
    11461 gatgccgttg cgggtgaaga gttcttgcag caggctgccg tcggcggccc cgttgaggat
    11521 ttggacgcga tgcacgccgc cttcgagcgc ggcaacggcg gacgaaatca gtcgtcgggt
    11581 ttcgctggcg gcgtgttccg ccagcgattg cgcttcctgt gccgagaggg tttcggcgag
    11641 cgtgccgtcg gggcgggaaa tgccgtctga aagggtcagg taaacgagtt tttcggcctg
    11701 aagcgagacg gcgacggaag cggcggcctg caccatatcg agattgaagg ttttgccgcc
    11761 gtaggaatgc ccgagcggcg gcatccagac gatattgccc gcgtcgagtt ggaaacggag
    11821 ggcggcggtg tcggttttgc ggataacgcc cgcgtattcc atatcggttc cgtcaatcac
    11881 gcctatcgga cgggcggtca ggaagttgcc cgatacgagc gggacggaag gcgcgcgcgc
    11941 gaatcctgaa acgctgccgc acaatgcggc ttcaaaacgg ctgcggacgg tgccggcaaa
    12001 ctgctgcgcc tgtccgagcg aggtttcgtc ggtaacgcgc aaaccccggc aataatgcgg
    12061 cgtgcggcct tgcgcggcgg cgaggcggtc gaggaagtgg tacgcgccgt ggatgaggac
    12121 gagtcggatg cccagttgcg acaacagccc gatgtcggcg gcgagcttat ttaaggtgcc
    12181 gccttcgagc aggcggccgt ctatgccggc gaccagtgtc gtgccgcgca tttggcggat
    12241 gtagggggcg gcttcgcgga agtgggcgac aaagctgtcg ggcgcgttca taggatgaag
    12301 aggtaggaaa gctgcataat gaggacgata agtgcgaaca gggttgcaat agtccagttg
    12361 agcccttcct gacgggcggg cggtacggaa acctgcggtg cggcggcagg tgcggcagcg
    12421 gcggttgcgg gcggcgtatc gtgcagggtt gtaccgccgt tgaggatatc ggcgatttcg
    12481 tcgcgggaaa tctgtttctt gctaatggcg tgcgtgccga tgcggtggac gagtttgaca
    12541 tccgaaacag cttcgggcaa atcgttgaat ataggttctt tcgtgcttgc cagatggtct
    12601 ttggctttaa acagcccttc gcatttttgg cagacgacga agccttgggc gacgttgagc
    12661 tgggtttctt tgacccagag gcgggttttg cagtgcggac agaaacaggc gggcatgatg
    12721 ttttctcggt gtgtgtcggt ttgatgccgt ctgaagcggc aggtggttcg gacggcatat
    12781 gctttatttt tattctacgc cgtactgccg gcggtaggtg cggacgggtt cgaggaactg
    12841 tccgaattcg gggttgtttt gcaacaggat aaacaaatcg ttcaggctgg cgatgggggc
    12901 gacgggcagg ccgtattgtt tttccacttc ctgaaccgcg ctcaattcac ccgtgccttt
    12961 ttccatgcgg tcgagcgcga tggcgacacc ggcgggggtt gcaccctccg cttcaatcag
    13021 tttgattgat tcgcgtacgg atgtgccggc ggaaatcacg tcgtcgataa tcagcacgcg
    13081 ccctttaagc ggcgcgccga ccaacacgcc gccttcgccg cggtctttgg cttctttgcg
    13141 gttgtaggca aacgggacgt tcacgccttt ttccgccagc atcatcgcgg ttgccgccgc
    13201 caaaataatg cctttgtagg cggggccgaa cagcatatcg aatcggatgc cgctttcaat
    13261 gatggattgt gcatagaatt ttgccagttg cagcgtggac gcgccgtcgt tgaagaggcc
    13321 ggcattgaag aaatagggcg accgccgtcc ggctttggtg gtaaattcgc cgaatttcaa
    13381 aacattttgg gcgagggaga atttgaggaa atcttggcgg aaatcagtca ttttgtgctt
    13441 tctgtcagat attgggacgc agttgcgatt ctaccgcccc gtgcggcgcg cttcaactgc
    13501 cgccgccttg ccgcaaggcg cgtttcaact gtttcaacac ctgccagttt cgggctttga
    13561 gttcgggttg gcgcaacagc cgggcgggat ggtcgatgat gaagaaggga cggctgccgc
    13621 acaaagtttc aatcatcgcc tgccgttcca ggttgacaaa cgcctgtccg aggaatagga
    13681 cggcgggggc gcggcagccg tcgagttccc cggcgatttg acccagcgca tccgcgacgg
    13741 cctgttcaga cggcatcggg ttaccgacgg cggcggtttt cacccaacag gttttgtgga
    13801 cataggcggc atccagtcct gcggctttga ggatgttgtc gagcaggatg cccgctttgc
    13861 cgtggaacag ttgcccgtaa accgcatcct cgatcggcgg acacaggctg acgacggcaa
    13921 gcttggtgat gcccgaagcg gcgggaacgg gggcgatgcc gtctgaaagg ccgggcgggg
    13981 gcgtttcggt ttcaggcgcg ggtttgcgcg tatgtacggc ggcggtttcc aacgctttca
    14041 tcgtttcgag ccgcgcctga acgttatggg gttgggaagg gcggatcgtg gcggcgcgga
    14101 cggtttggcg acgtgcctgt gccggggttg cgggtgtgtt tttgggcggc aggacggcgg
    14161 cggcctgttt cagccacatc ggacccaagc ccaaagcctc gtgcaggtgg aggtagcgcg
    14221 cgcttaacat attttctcca ttaagacggc atcttcggtt tgcccgtcgg cggtgcagta
    14281 atagtttttc cgcctgcccg caatgctgaa gccgtgtttg gtatacagtg cctgtgcggc
    14341 ggcgttgcct gcgcggactt cgagcagcag gcgttgcgtg ccttcgggca gatgtgcgta
    14401 ccaatattcg agcagggcgg acgcaacgcc ccgtcggcgg cattcgggcg cggtggcaat
    14461 caggtgcagt tcggattcgt cgggcaggtt ttgccaaacg ataaaggcgg caatcctgcc
    14521 gtctttttcc gcaaggaaaa cctgttcgga cggcgaaaca agcgcggact caaattggcg
    14581 ctgcgtccac gcggacgggt tgcagacggt atcgagcgcg gccagtgcgg cgcagtcgga
    14641 cggtaaggct gggcggatgt tcatgttcgt gccttccgtt ccgcctgttc tttggcagtc
    14701 agggcgattt tgttgcggac gtagagcagt tcggcgtgtg ccgcgccagt tgcgggatag
    14761 ccgccgccga gggcgagcgc gagaaaatcg gcggcggtcg gcatatcggg tttgcctgag
    14821 aagggcggac ggttttccag tgcgaacgca ctgccgatgc cgtctgaaaa gacgtacccc
    14881 tcggggaggg caatgtctgc cgccctaccg acttgataat cgctcaaacg gcggcggttc
    14941 agcgtgtcga accacgcata aaacacttcg cccatacgcg cgtccgcagc ggcgagtatg
    15001 cagctttgcg gcggcggcag cgaggcggcg gcatcgagcg tggggatgcc gattaaaggc
    15061 gtgtcgaacg gcgttgccaa accttgcgcc acgccgatgc cgatacgcag tccggtaaac
    15121 gcgccggggc ctttcgcata aacaatcgcc cccaaatcgg cggcggtaat gcccgcattt
    15181 cggaataggg tgcggatttc cggcaggatc agttcggact ggcggatgcc gacttcctga
    15241 tggaacagac ggatttcgcc gtcggcgcgc agcgcgagcg acaaatagga agttccggta
    15301 tcgacggcga ggacggggcg gttgaaatcg gcttgcatgg tgtggttctc gttggttcag
    15361 acggcattat atagtgaaat cggcttgcct gccgtgccgt cgtgtcctag ggcggtatgg
    15421 cgcaaaaatg ccgtccgaac ggtaaattat cgtgttcgga cggcattttt caaatactac
    15481 tgtttgtcgg cgatgccgat ttcgtgaacc ttttccccta tcttcacggt tgccgagcca
    15541 gcgatttctt gggcgcggtc gccgaaaagg gcgaggcggt aagtgccttt ctcttcgccg
    15601 ccgtagcgcg tgtcgcccaa aatgacggcg tgtgattttt catctgcttt gagttcggcg
    15661 gaggcaagct caacattctg ctcgggtgtt ttcaggtgtt cgattttgcc gtgtccctgt
    15721 ttggcggcga aatctatggt ataggtcagt tttccgccgg catcgtcgga gctgaatgct
    15781 ttgccgtgat actcggcttt gccgtcaggc agttggttga aggcggtatg ttctccgccc
    15841 aaatcgctga caaggaagga gcgttggttt atcaggctgt cgattttgtc ggggttgttg
    15901 attttttcaa tccgtagggc aacgacggcg gagtgatcct gtttgtatat ttgaaattcg
    15961 ccgcttgcca gtgtgatggt ttgtccgtcc acttcgattt tttgcacgaa gtcgaagcgg
    16021 ctgattttgt cgttcttcag tttgcccgtg ttgaggctgt tgtctttgcc gccggctttg
    16081 aaagtttttt ccgcaccttg tgccgacagg gtcagtgttc cgttttgggg aatggaggct
    16141 tccaatgtta gggatttcaa acctttgtct ttatggtcga gcggcgcggt taatgcatcg
    16201 gcaagccccg tgccgatgtc ggcggcgaca ccaccgcctc cgcccctccg ctgctgcagg
    16261 cggtcagaat cagggccggc ggtcaaagaa aggcagcaga aggtagttcg gttcacaggt
    16321 ttactcctag tcatacacag aatagataat atataaacgt tttggttatg gtatcttttt
    16381 ttgcatactg catcaatgag gcaggtcaga gaagcaaaaa tcaaatgccg tccgaacggc
    16441 gttcagacgg cattttgttt acaggcaacc tgttatttga cgatttggtt caattcgccc
    16501 ttggcataac ggcttgccat tttttccaac gaaacaggtt tgattttgcc tgcctgacct
    16561 tcgcaaccga acgcaagata acggtcgagg cagatttgct tcatcgcttc aatggttttg
    16621 cccaagtatt tgcgcggatc aaagtcggac gggttttcgg caaggtagcg gcgtaccgcg
    16681 ccggtggaag cgaggcgcag gtcggtatcg atgttgactt tgcgcacgcc gtgtttgatg
    16741 ccttcgacga tttcttcaac cggcacgccg taggtttcgc cgatattgcc gccgtattcg
    16801 ttgatgactt tcagccattc ttgcggaacg gagctggagc cgtgcatcac gatgtgtgta
    16861 ttgggcaggg cttggtggat ttccttgatg cggtcgatac gcaatacgtc gcctgtgggc
    16921 ggacgggtga atttgtatgc gccgtggctg gtgccgacgg caatcgccaa tgcgtcaacg
    16981 ccggtatctt taacgaaacg cacggcatct tcaacgctgg tgagcatttg gtcgtgtgag
    17041 agtttgcctg ccgcgcccac tccgtcttct tcgcctgctt cgccggtttc gaggttgccc
    17101 aatacgccga tttcgccttc gacggacacg ccgcaggcgt gggagaagtt gacgacggta
    17161 cgggtggcgt tgacgttgta ttcgtaagaa gaaggggttt tgccgtcttc gagcaaagag
    17221 ccgtccatca tcacggagga gaagcccagt tggatggagc gttggcacac gtcgggcgat
    17281 gcgccgtggt cttggtgcat cacgacgggg atgtgcggaa attcttcgac tgccgccaga
    17341 atcaggtggc gcaaaaacgg cgcgcccgcg tatttgcgcg cacctgcgct cgcctgtacg
    17401 atgacgggcg cgttgacttg gtcggcggct tccataatgg cgcgcatttg ttcgaggttg
    17461 ttgacgttga acgcgggcag gccgtagctg ttttcggcgg cgtggtcaag cagttggcgc
    17521 atggatacga gtgccatttg tgtctccttg ggcaataggt aaataaggcg gattataatg
    17581 ttttttacgg caaaaaacca taaacggctc attgatttta tattaacgat aacggcgggc
    17641 agatggggcg gattttgggc ggttcgggat tttggtgtgt tgtttatgat aatgttttca
    17701 ctggtttttt gatatttgtg tgggacggtt atggttttgg acgggtttgc ggcgtatttt
    17761 gacgcttatt tggaaaacat cgtgcgcgag ggcaagtcgg agcacacggt tgcggcatac
    17821 cggcgcgatt tggaagaact gtttgcactg ttggcacaaa tgccgtctga agatgcaggc
    17881 ggcgtgccgc aggacttgtc gcggcgcgat tttacggcgg cgttgcggcg gctgtcgcag
    17941 cgcggtttgg acggtcggac gctggcgcgc aagctgtcgg cgtggcggca gtattgcgcc
    18001 tggctggtta aacgcgggct gatgcgcgcc gacccgaccg ccgacatcaa accgccgaag
    18061 cagcccgagc gcgtacccaa agccctgccg caggaatggc tgaaccggat gttggatttg
    18121 cccgtggacg gcggcgaccc gctggcggtg cgcgaccacg cgctgttcga gctgatgtac
    18181 ggcagcggtt tgcgcgtgag cgagatacac ggcttgaatg cagatgatgt atatttggac
    18241 gaagcgtggg tacacgttac cggcaaaggg cgcaagcagc gtcaggttcc gctgaccggc
    18301 aaaagcgtgg aagccttgaa aaactatctg ccgctgcgtc agacggcatc ggacggcaaa
    18361 gccctgttta ccggcaggaa cggcacgcgc ctgagccaac gccaaatcca aaaacgcctc
    18421 gaatcgtggg cggcgcaata cggcgacggc aggcacgttt cgccgcatat gatgcgccac
    18481 agctacgccg gccacctgtt gcaggcttcg cgcgacatca gggcggtgca ggagctgctc
    18541 ggacacagca gcctttcgac cacgcagatt tataccaagc tcgatttcga ccacatcgcc
    18601 cgcctctatg acgaagccca cccgcgcgcc aagcggcagg acgaatgacg tacggcaaaa
    18661 tcagccgtca gccgcacgct cttgatatat aattgaccgt tgcacccgga cgacacataa
    18721 aaaagacaca ccatgaaccc aagcccccta ctcgacctga ttgacagccc gcaagatttg
    18781 cgccgcctgg acaaaaaaca gctgccgcgc cttgccggcg agttgcgcgc ctttctgctg
    18841 gaatctgtcg ggcagaccgg cgggcatttc gccagcaatc tgggtgcggt cgaactgacc
    18901 atcgccctgc actatgtgta cgacacgccc gaagacaagc tggtgtggga tgtcggacac
    18961 caaagctacc cgcacaaaat cctgacaggc aggaaaaacc agatgcacac catgcgccaa
    19021 tacggcggtt tggcgggttt tccgaaacgt tgcgagtccg agtacgacgc gttcggcgtg
    19081 gggcattcct ccacctccat cggcgcggct ttgggcatgg cggcggcgga caaactcttg
    19141 ggcggcgacc gccgcagcgt cgccatcatc ggcgacggcg cgatgacggc ggggcaggcg
    19201 tttgaagcct tgaattgcgc gggcgatatg gatgtggatt tgctggtcgt cctcaacgac
    19261 aacgaaatgt cgatttcccc caacgtcggc gcgttgccca aatatcttgc cagcaacgtc
    19321 gtgcgcgata tgcacggact gttgagtacc gtcaaagcgc aaacgggcaa ggtattagac
    19381 aaaatacccg gcgcgatgga gtttgcccaa aaagtcgaac acaaaatcaa aacccttgcc
    19441 gaagaagccg aacacgccaa acagtcgctg tcgctgtttg aaaatttcgg cttccgctac
    19501 accggccccg tggacggaca caacgtcgag aatctggtgg acgtattgaa agacttgcgc
    19561 agccgcaaag gccctcagtt gctgcacgtc atcaccaaaa agggcaacgg ctacaaactc
    19621 gccgaaaacg accccgtcaa ataccacgcc gtcgccaacc tgcctaaaga aggcggggcg
    19681 caaatgccgt ctgaaaaaga acccaagccc gccgccaaac cgacctatac ccaagtattc
    19741 ggcaaatggc tgtgcgaccg ggcggcggca gattcccgac tggttgcgat tacccccgcc
    19801 atgcgcgagg gcagcggact ggtggagttt gaacaacgat tccccgaccg ctatttcgat
    19861 gtcggcatcg ccgagcagca cgccgttacc tttgccggcg gtttggcgtg cgaaggcatg
    19921 aagcccgtcg tggcgattta ttccaccttt ttacaacgcg cctacgacca actggtgcac
    19981 gacatcgccc tgcaaaacct gcccgttttg tttgccgtcg accgtgcggg catcgtcggc
    20041 gcggacggtc cgacccatgc cggcttgtac gatttgagct tcttgcgctg tgtgccgaac
    20101 atgattgttg ccgcgccgag cgatgaaaac gaatgccgcc tgctgctttc gacctgctat
    20161 caggcggatg cgcccgccgc cgtccgctat ccgcgcggca cgggtacggg cgcgccggtt
    20221 tcagacggca tggaaaccgt ggaaatcggc aagggcatta tccgccgcga aggtgagaaa
    20281 accgccttca ttgccttcgg cagtatggtc gccaccgcat tggcggttgc cgaaaaactg
    20341 aacgccaccg tcgccgatat gcgcttcgtc aaaccgatag acgaagagtt gattgtccgc
    20401 cttgcccgaa gccacgaccg catcgttacc cttgaagaaa acgccgaaca gggcggcgca
    20461 ggcggcgcgg tcttggaagt gttggcgaaa cacggcatct gcaaacccgt tttgcttttg
    20521 ggcgttgccg ataccgtaac cgaacacggc gatccgaaaa aacttttgga cgatttgggt
    20581 ttgagtgccg aagcggtgga acgccgggtg cgcgagtggc tgccggaccg tgatgcggca
    20641 aattaaaccg cttgaccgcg ccgtcgttat cgggcggcgt ttttaaacgg cgtttgtttc
    20701 tgcggttttt ttattgaaac cccgcaggcg gcaggaaggg ttcgggcggc ggctttcggg
    20761 cggtgcttgg tgtgccgttg cgcgtttgga aatttattcc gcttgtccgt ataacggcgg
    20821 cggtgccgtc tgccgataca aggcaaaatg ccgtctgaaa cgcttcaggc ggcatttttc
    20881 ggcgtgaggg ttttaggctt cgacaatttt gccgcgcagg gaaaaggtgt aggcttcggt
    20941 gatttccaaa tcgatcattt ggttgatcat gtcgggcgtg ccggtaaagt tgacgacgcg
    21001 gttgttggcg gtacgggctt ggagctggtc ggggtctttt ttggagatgc cttcgaccag
    21061 gcagcgttga accgtgccga tcatggtttg gttgatgcgg gcggtttcgg cttcgatgac
    21121 ttcgttcaag gcttcgaggc ggcgcacttt ttcttcgtgc ggcgtgtcgt ccggcaggtt
    21181 ggcggcaggc gtgccggggc gcgggctgta aataaacacg aagctcaagt cgaaggcaat
    21241 gtctttcacc agtttcaagg tttgctcgaa ctcgcgttcg gtctcgccgg ggaaaccgac
    21301 gatgaagtcg ctgctcaggc acaaatcagg acggatggcg cgcagtttgc ggatgatgga
    21361 tttgtattcc aaagcggtgt agccgcgttt catcgcgctc aatacgcggt cggaaccgct
    21421 ttgaatcggc aggtgcaggt gggaaaccag tttgggcagg tcgcggtagc actcgataat
    21481 cgagtcggta aactcgcgcg ggtggctggt ggtgaagcgc atacgttcga tgccggggat
    21541 ttcgtggacg atacgcagca gggtggcgaa gtcgcagatt tcgccgtcgt ccatttcgcc
    21601 gcgataggca ttgacgtttt gtcccaagag gttgatttct ttcacgcctt gctgggcaag
    21661 gttggcgatt tcggccaata cgtcgttgag cgggcgggag aattcttcgc cgcgcgtgta
    21721 ggggacgacg cagaaggagc agtatttgga acagccttcc ataatcgaca caaatgccgc
    21781 gccgccttcg acgcgggcgg gcggcaggtg gtcgaatttt tcgatttcgg ggaaggaaat
    21841 atcgacttgc gacagcccgc tggtttcttt gtccacaatc attttgggca ggcggtgcag
    21901 cgtttgcgga ccgaaaacca cgtcaacata aggcgcgcgt ttgatgatgt tttcgccttc
    21961 ttgcgaggcg acgcagccgg caacgccgat gatgaggccg gggttttttt ctttgagcgg
    22021 acggacgcgc cccaagtcgg agaacacttt ttcctgtgct ttttcgcgca cggaacaggt
    22081 gttgaacaag atgatgtcgg cttcgtcggc ttgggtaacc tgttcgatgc cgccgtgttc
    22141 ttcggcaagg acggacagca ttttttcgct gtcgtactcg ttcatctggc agccgaaggt
    22201 gcggataaat acttttttca tggtttgtgt ctttctcagg cagccgtaat cgcggggctg
    22261 atggttgttg gaatgaaaaa atttcagacg gcacgacgat gccgtctgaa aatcggtgcg
    22321 gattatagca cgatgtgggt ttgggaggca aaatattgtt ttaaaatatg aatttaatcg
    22381 gtcggaacgg ctgtataatg tttggcttta atgggaggtg tgtatgaaac cggttgtatg
    22441 ggcggcattg ctgctgtgtg cgtgtaccag caatttcggc gacagggaac atcagttcct
    22501 gcgttatagt ggattaaatt taaaccagta cagcgttgcc tcgccttgcc gtactatttg
    22561 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata tcagacggaa
    22621 gagggaatcg cacaaacggt tgttaaaggc aaaacgaccc gggcggaggt agaggcacgt
    22681 ttcgggaagc gcaacccttt cggctgttat gcctatcacg aggtcagcct gccgatttat
    22741 aattttttgc cgaccaattt catctatatg aaatcggagc ggcggcattg ggaatggtgc
    22801 gcggattacg acggggaggg agtcgtccgg gactaccgct ttacacataa agtggaaaaa
    22861 gacgagcgtt ccgtcatccg ggatacggtt ggcgtaatcc gcaaagaagc gggcaaatcc
    22921 ttgtcgcaac ctgaaaaatg ataaaatggg gctttctgct tccaagcccg aaacctgccg
    22981 ttcagacggc atttgaggat aaatatgaac cgtaacgaaa ttttattcga ccgcgccaag
    23041 gcggtcattc ccggcggcgt gaattcgccc gtgcgcgcat tcggcagcgt cggcggcgtg
    23101 ccgcgcttca tcaaaaaagc cgaaggcgcg tatgtttggg acgaaaacgg cacgcgctac
    23161 accgattatg tcggctcttg ggggcctgcg attgtcggac acgcgcatcc cgaagtcgtc
    23221 gaagccgtgc gcgaagctgc gttgggcggt ttgtcgttcg gcgcgcccac cgaaggcgaa
    23281 atcgccattg ccgaacaaat tgccgaaatt atgccgtctg tcgaacggct gcgcctcgtc
    23341 agctccggca cggaagcgac gatgactgcc atccgtctgg cacgcggttt taccggccgc
    23401 gacaaaatca tcaaatttga aggctgctac cacggccatt ccgacagcct gttggtgaaa
    23461 gcaggcagcg gtctgcttac cttcggcaat ccttcttccg ccggtgtgcc tgccgacttt
    23521 accaaacata ctttggtact cgaatacaac aacatcgccc aactcgaaga agcctttgcc
    23581 caaagcggcg acgaaatcgc ctgcgtgatt gtcgaaccct tcgtcggcaa tatgaacctc
    23641 gtccgcccga ccgaagcctt tgtcaaagcc ttgcgcggat tgaccgaaaa acacggcgcg
    23701 gtgttgattt acgacgaagt gatgaccggt ttccgcgtcg cgctcggcgg cgcgcagtcg
    23761 ctgcacggca tcacgcccga cctgaccacg atgggcaaag tcatcggcgg cggtatgccg
    23821 cttgccgcgt tcggcggacg caaagacatc atggaatgta tttccccgtt gggcggcgtg
    23881 tatcaggcag gtacattatc aggcaacccg attgccgtcg ccgccggctt gaaaacgctg
    23941 gaaatcatcc agcgcgaagg cttctatgaa aacctgaccg ccttgacaca acgccttgcc
    24001 aacggtattg ccgccgccaa agcgcacggt atcgagtttg ccgccgacag cgtgggcggt
    24061 atgttcggtc tgtatttcgc cgcacacgtg ccgcgaaact atgccgatat ggcgcgctcc
    24121 aatatcgacg ctttcaaacg cttcttccac ggcatgctcg accgcggcat tgccttcggc
    24181 ccgtccgctt atgaagcagg tttcgtttcc gccgcgcata cgcccgagct gattgacgaa
    24241 acggttgcgg ttgcggttga agtgttcaag gcgatggctg catgatgttt tgacggacag
    24301 agtttctctg ttcgatttgt ttggcagatt gaagtaagaa tgcacaccgc cgtcatttcc
    24361 gcgcaggcgg gaatccggac ctttcagttt ctgtaatgat tgaaaataac ggcaagcccg
    24421 accttccgga ttcccgcctg cgcgggaatg acgggcgtgt acatttttga tttcaatcta
    24481 ctgtaaaaat gccgtctgaa atatatagtc aattaaaatc aaaataggac agtagtgcat
    24541 cgtcaaatcg ggcgtaatca gacaaaacgg ttcgcagata ccgcttaata ttcgcccaca
    24601 ctttctcaat cgggttgggc tcgggcgaa
//
LOCUS       DAXFZR010000021        24498 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000021 DAXFZR010000000
VERSION     DAXFZR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24498
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.021"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            447..848
                     /locus_tag="ACFJAS_000750"
     CDS             447..848
                     /locus_tag="ACFJAS_000750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982051.1"
                     /translation="MNFSNEQKLFNLYAQLGQIAFTEIRHLAFGRTSLHQNTPAFPKS
                     VPTEALLQNLFVAGQLAEAMHNLPCNLHDGTYQQYLTLRNLWQFIEKNPNYADCFFPT
                     IAKIIQLNHQELAEIDADFVATIAGKYRRVD"
     gene            986..1537
                     /locus_tag="ACFJAS_000751"
     CDS             986..1537
                     /locus_tag="ACFJAS_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009116112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFC3982052.1"
                     /translation="MKVKPFLAVSALTAALLAPMTASADDNLLTGDVRLACEAVLCLS
                     SGNRPSECAPSIKRYFSINHKKLSDTINARRSFLNLCPSDNAQGMPQLIDAIANGAGR
                     CDAAALNRRGHYTGSSRDGTRRFIVNTQKPDYCKAYENHAWTRVATTKLEPIYCNYTT
                     GDTPWRKKRVEKYQCGQKWVDVQ"
     gene            1663..2409
                     /locus_tag="ACFJAS_000752"
     CDS             1663..2409
                     /locus_tag="ACFJAS_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobQ"
                     /protein_id="HFC3982053.1"
                     /translation="MKEVHFTMQGKGGAGKSFITSILAQYLKKRIGDKNVTCFDTDPI
                     NPTLSRYAALNPTIIHLLEDGKINSRHFDELMEIIETQDGIGVVDTGAGTFVPLMYYF
                     SENHIAEYLFDCDVNLVIHVPIVGGQAFNDCLVVLDQIMSSMGCDVVIWLNHFQGKIT
                     NFTETKVYQENKEHIIGIMEIQNKDSDTFGKDLETMTKMNLTFDEVQNSTVFKGMERN
                     RLKHFSREMFEALDNIPFIATLKDDDEPSS"
     gene            2393..2854
                     /locus_tag="ACFJAS_000753"
     CDS             2393..2854
                     /locus_tag="ACFJAS_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC3982054.1"
                     /translation="MSQVHETEIRQLIADVMRDTGIRIDADDPIVAMLFAQKRELAQF
                     LQQSGDEQNAQRERFLADFQTHSESIITAAAELQSQKQELLASLLQANAQDRAEMEQK
                     LFGSISRRVQTQFQAQATELAKHISGSLKMGVMMWAAVQMLIFAVMIFLLK"
     gene            2894..3235
                     /locus_tag="ACFJAS_000754"
     CDS             2894..3235
                     /locus_tag="ACFJAS_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003759548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3982055.1"
                     /translation="MKTYTVNEAAEYCRCHPETLREYIRAGKLAAGKVGRAYCIRQTK
                     LDEFLAQLENDAVQASLIKRSGQKCQKIQMDCTNATTYGTWTSERRAASELDALLAHK
                     TNKKPKNYAPS"
     gene            3145..4152
                     /locus_tag="ACFJAS_000755"
     CDS             3145..4152
                     /locus_tag="ACFJAS_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3982056.1"
                     /translation="MDIRTPGGERVRRSTGTQDKQKAQELRAKLEHEFWQQSRLGVKP
                     KKLWDEAALQWLKEKEYDKKSIQDDISRLRNLPRLRGIFLDELNRETIMAVVEKLPCG
                     NSTKNRYLALIRAILYKARDEWLWLDEVPKLKLYKEPGNRIRWLKPDEAHRLIDALPD
                     NFWRQMAIFSLCTGLRQSNVFGLKWEQVDLDKRIAWIHPDEAKAARAIGVPLNQTAVQ
                     VLSERAGIHTTYVFTNSVNNPICGLDSRVWERSLKKAHIKNFRWHDLRHTWASWLVQS
                     GVPLLALKEMGGWEKLDMVMRYAHLATEHLQSHADILDTMHTLDTKWTHFKTKGQKEK
                     A"
     gene            complement(4177..4252)
                     /locus_tag="ACFJAS_000756"
     tRNA            complement(4177..4252)
                     /locus_tag="ACFJAS_000756"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4217..4219),aa:Asn,seq:gtt)
     gene            complement(4344..4703)
                     /locus_tag="ACFJAS_000757"
     CDS             complement(4344..4703)
                     /locus_tag="ACFJAS_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC3982057.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSVYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(4724..5366)
                     /locus_tag="ACFJAS_000758"
                     /pseudo
     CDS             complement(4724..5366)
                     /locus_tag="ACFJAS_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            complement(5368..5903)
                     /locus_tag="ACFJAS_000759"
                     /pseudo
     CDS             complement(5368..5903)
                     /locus_tag="ACFJAS_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            complement(5915..7013)
                     /locus_tag="ACFJAS_000760"
                     /pseudo
     CDS             complement(5915..7013)
                     /locus_tag="ACFJAS_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(7016..8453)
                     /locus_tag="ACFJAS_000761"
                     /pseudo
     CDS             complement(7016..8453)
                     /locus_tag="ACFJAS_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(8674..9456)
                     /gene="bioC"
                     /locus_tag="ACFJAS_000762"
     CDS             complement(8674..9456)
                     /gene="bioC"
                     /locus_tag="ACFJAS_000762"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC3982058.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            complement(9444..10091)
                     /locus_tag="ACFJAS_000763"
     CDS             complement(9444..10091)
                     /locus_tag="ACFJAS_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC3982059.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            complement(10101..11243)
                     /gene="bioF"
                     /locus_tag="ACFJAS_000764"
     CDS             complement(10101..11243)
                     /gene="bioF"
                     /locus_tag="ACFJAS_000764"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC3982060.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            complement(11265..11402)
                     /locus_tag="ACFJAS_000765"
     CDS             complement(11265..11402)
                     /locus_tag="ACFJAS_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC3982061.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGCFR
                     L"
     gene            11466..12542
                     /locus_tag="ACFJAS_000766"
     CDS             11466..12542
                     /locus_tag="ACFJAS_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFC3982062.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(12751..14217)
                     /locus_tag="ACFJAS_000767"
     CDS             complement(12751..14217)
                     /locus_tag="ACFJAS_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC3982063.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(14415..15338)
                     /gene="speB"
                     /locus_tag="ACFJAS_000768"
     CDS             complement(14415..15338)
                     /gene="speB"
                     /locus_tag="ACFJAS_000768"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC3982064.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(15437..17329)
                     /gene="speA"
                     /locus_tag="ACFJAS_000769"
     CDS             complement(15437..17329)
                     /gene="speA"
                     /locus_tag="ACFJAS_000769"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC3982065.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(17496..17780)
                     /locus_tag="ACFJAS_000770"
     CDS             complement(17496..17780)
                     /locus_tag="ACFJAS_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC3982066.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(17930..19738)
                     /gene="aspS"
                     /locus_tag="ACFJAS_000771"
     CDS             complement(17930..19738)
                     /gene="aspS"
                     /locus_tag="ACFJAS_000771"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC3982067.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGIAGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVATGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGGFPRMPYSEA
                     VFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            complement(19796..20497)
                     /locus_tag="ACFJAS_000772"
     CDS             complement(19796..20497)
                     /locus_tag="ACFJAS_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC3982068.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            complement(20639..21787)
                     /locus_tag="ACFJAS_000773"
     CDS             complement(20639..21787)
                     /locus_tag="ACFJAS_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC3982069.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            complement(21947..22210)
                     /gene="rpsT"
                     /locus_tag="ACFJAS_000774"
     CDS             complement(21947..22210)
                     /gene="rpsT"
                     /locus_tag="ACFJAS_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC3982070.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            22512..23654
                     /locus_tag="ACFJAS_000775"
     CDS             22512..23654
                     /locus_tag="ACFJAS_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC3982071.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSADAKKLSVSLWQKIKVGTN"
BASE COUNT         6450 a         6124 c         5741 g         6183 t
ORIGIN      
        1 acaaatttgt ctattttaga tatactaaaa atcaacaaga gtctattatt gaatgacaac
       61 agttctttaa caatctagta gcgcagcaac ccactagcct gattggtttc aggcgagcaa
      121 gtcgttaagc ggttgcaggc ggcgttcaat ccgcccaaat acaaatgtcg tgcatacggg
      181 tagcgcgttg tgcgtccgtg aacaggcggc aatttgaacg tatcttgtta atcggggcgt
      241 atccccgatg ctgggattat ctacggacaa atcctaacgc catttgttgc accaagtggc
      301 gaatacacgt tttcaggcag cgttgagatt actccgttgc ctgtgatgtg ttttgtgtgt
      361 tttcacaatc aagcctgttc tgctcggcaa aatcaaatag cggagcaggt ttctttgtga
      421 gaattatttt cttgaaaggg tgtgcaatga atttttcaaa tgaacaaaag ctgtttaatt
      481 tatatgctca attaggacaa attgcattca cagaaatccg tcatttggca tttgggcgaa
      541 ccagtttgca tcaaaacacg cctgcatttc ccaaaagcgt accgaccgaa gcgttattgc
      601 aaaatctgtt tgtggcaggg cagttggcgg aagcgatgca caatctgcct tgtaatttgc
      661 atgatggcac ttatcagcaa tatttaacct tacgcaattt gtggcaattc attgaaaaaa
      721 atccaaatta tgcagattgc tttttcccta caattgcgaa aattatccaa ttaaatcatc
      781 aggaacttgc ggaaatagat gctgattttg ttgccaccat tgctggtaaa tatcggcgcg
      841 tggattgatt ttctgctcgc gcttgaccgc gattatttgc aaatggcgat tgaatacgtc
      901 aatgaattag aaatttagtt tcaggcagcc tgaatccatt ttcagactgc ctttttttct
      961 gaaaaaaaca atgggagttt caaaaatgaa agttaaacca tttttagcgg tttccgcgct
     1021 gactgccgcg ctgcttgcgc ctatgaccgc cagcgcagac gataatttgc tgacaggcga
     1081 tgttcgcttg gcgtgtgaag cggtactgtg tttgtctagt ggtaatcgcc caagtgaatg
     1141 tgcgccgtct atcaaacgct atttctctat caatcataag aaattgagcg ataccatcaa
     1201 tgctcgccgc agttttttaa atttatgtcc atcggataat gctcaaggaa tgccacaact
     1261 gattgatgcg attgccaatg gtgcagggcg ttgtgatgcg gctgctctca atcgtagggg
     1321 gcattacaca ggcagtagcc gtgatggtac acgccgtttt attgtcaata cgcaaaaacc
     1381 tgattattgc aaagcgtatg aaaatcatgc gtggacgcgt gttgcgacta ctaaattaga
     1441 accgatttac tgtaattaca ccacaggcga cacaccttgg cggaaaaaac gtgtggaaaa
     1501 ataccaatgt gggcaaaaat gggttgatgt ccaatagttt cactttgctt gatggcagtt
     1561 ttaccgttcg cattggacgg tttttttatg cgtttcaggc agcctgaacg gaatatttca
     1621 agctacctga atagttaaat ctcattgaaa agaaagatga ttatgaaaga agtacatttt
     1681 accatgcaag gcaaaggcgg tgctggcaag tcgtttatca cttctatttt ggcgcaatat
     1741 ctgaaaaaac gcatcggcga taaaaacgtt acttgttttg acaccgaccc gattaacccg
     1801 acattgagcc gctatgccgc tctcaatccc accattattc atttgttgga agacggcaaa
     1861 attaacagcc gccattttga tgaactcatg gaaatcattg aaacacaaga cggcatcggc
     1921 gtagtagata caggcgcagg gacattcgta ccattgatgt attatttttc tgaaaaccat
     1981 attgcagaat atctgtttga ttgtgatgtg aatttggtta ttcatgtacc gattgttggc
     2041 ggtcaggcgt ttaatgattg cttggtggtg ctagaccaaa tcatgagcag catgggttgt
     2101 gatgtcgtca tttggctgaa ccattttcaa ggcaaaatca ctaatttcac agaaacaaag
     2161 gtttatcaag aaaacaaaga acacatcatc ggcattatgg aaatccaaaa taaagattcc
     2221 gacacgttcg gcaaagattt ggaaaccatg accaaaatga acttaacctt tgatgaagtg
     2281 caaaattcca ccgtattcaa aggtatggaa cgcaaccgct tgaaacactt ttcgcgtgaa
     2341 atgtttgaag cattggacaa tattccattc attgccactc tgaaagatga tgatgagcca
     2401 agttcatgaa acagaaatcc gccaactgat tgccgatgtg atgcgcgata caggcatccg
     2461 tattgatgcc gatgacccga ttgttgccat gctgtttgcc caaaagcgcg aattggcgca
     2521 atttttgcag caatccggcg atgagcagaa cgcgcaacgt gaacgatttt tagcagattt
     2581 tcaaacgcat tccgaaagca tcatcacggc agctgcagaa ttgcagagtc aaaagcagga
     2641 attgctcgca tcactattgc aagccaacgc ccaagaccgc gctgaaatgg agcagaaact
     2701 gtttggcagc atcagccggc gtgttcaaac gcaatttcag gcgcaagcaa ccgagttggc
     2761 aaaacacatt tcaggcagcc tgaaaatggg cgtgatgatg tgggcggcgg tgcaaatgct
     2821 gatttttgcc gtaatgattt ttttattgaa atgacaacag gcagcttgtt gcaggctgtc
     2881 tgaaaggaaa accatgaaaa cctacaccgt aaacgaagcc gccgaatatt gccgctgcca
     2941 ccctgaaact ttgcgcgaat atatccgggc agggaaactg gccgccggca aagtaggacg
     3001 cgcttactgt attcgccaaa caaaacttga cgaattttta gcacaacttg aaaatgatgc
     3061 cgtacaggca tcactcatca aacggagtgg acaaaaatgt caaaaaattc aaatggattg
     3121 taccaacgca acaacatatg gtacttggac atcagaacgc cgggcggcga gcgagttaga
     3181 cgctctactg gcacacaaga caaacaaaaa gcccaagaat tacgcgccaa gttagaacac
     3241 gaattttggc aacaaagccg tttgggcgtg aaacccaaaa aactgtggga cgaagccgcg
     3301 ttgcaatggc tcaaagaaaa agagtatgac aaaaaaagca ttcaagacga catcagccgt
     3361 ttacgcaatt taccccggct tcggggtatt tttttggacg agttgaaccg tgaaaccatt
     3421 atggcagttg tggaaaaatt accgtgcggc aacagcacta aaaaccgcta tctagcactc
     3481 attcgggcga ttttgtacaa agcgcgtgat gaatggctgt ggttggacga agtgccaaag
     3541 ttaaaattgt ataaagaacc cggcaaccgc attcgttggc tgaaacccga tgaagcacat
     3601 cgtttgattg atgcgctgcc tgacaatttt tggcggcaaa tggcgatttt cagcttgtgt
     3661 acaggattgc gccaaagcaa tgtgttcggg ctgaaatggg aacaagtgga tttagacaaa
     3721 cgcattgcgt ggattcatcc cgatgaagcc aaagcagccc gagcgatcgg cgtaccgctc
     3781 aatcaaacgg cggtacaagt tttgtcagaa agagcgggta ttcacacaac ctacgttttt
     3841 accaatagcg tgaataatcc aatctgcgga ctggatagcc gcgtatggga acgcagtctg
     3901 aaaaaagcgc acatcaaaaa tttcagatgg catgatttgc gccatacttg ggcaagttgg
     3961 ttggtgcaaa gcggcgttcc gcttttggct ttgaaagaaa tgggcggttg ggaaaagctg
     4021 gatatggtga tgcgatacgc gcatttagcc accgaacatt tgcaaagtca tgcggatatt
     4081 ttggacacaa tgcacacttt ggacacaaaa tggacacact ttaaaacaaa ggggcaaaag
     4141 gaaaaagcct aaacaattgt ttaggctttg ataatttggc tccccgacct gggctcgaac
     4201 cagggacctg cggattaaca gtccgtcgct ctaccgactg agctatcggg gagtaaacgc
     4261 gaataataaa acagaaatac cgaacgtgtc aatataaatt gacaatcgat tgcacttatc
     4321 tgcaagcccg caatctttat atgttatatg gcttccgcaa tatgccttgc cgcacgtttg
     4381 gcatccaata ggattaaacc aagtctgccg gtttcttttg ccaccagcac caacacggca
     4441 tctttacccg cctgacttaa aaggatatag actgattttc ctttaatcat cacttgttcc
     4501 aattccccgc aggcgagttc ctgcaccgag cgactcccca aagcaagcaa agtggcagaa
     4561 atcgccccta ccctgtccga attcaaatgt gaaggaagca ttgtcgccat cggcaatccg
     4621 tcggttgaga taacggcaga cgcgataata tccgtagatg tattatttaa atcttcaagg
     4681 attgaaatca ataattgctg cataatccct cctgtcccaa gttttacacg cggttgctgt
     4741 aacggcggta taaaatcctt accaaagtaa caaatgcctc tttgctcaaa tcgggaatgc
     4801 cggcgataac caaaataaat ttggttgaac cgatatacaa tgggaaaaat gtcaattcgc
     4861 tctgaccgga aggatcgcaa acgccccaag cgttattgtt gatatacagg ttgttcctaa
     4921 tcagcagccg gtatttcttt tccatctgtg cgacttctgc cgccaacaac cccaactctt
     4981 ccgccgactc atgatggaaa ttggcgttgg caagatacag accgttccga tcgaccaata
     5041 atgccttacc gctgccggac aattgttcca tcagcaacgg caattgctcg tccgacaaat
     5101 tgatgccgtc tgaatgaccg ttttcatcgc catagaggaa ttcgagtttt tgcaaacggt
     5161 acaacaggtt caaagcggta tcgatgtcgg cggtgtccgc ccgcgagcag cttctcactg
     5221 ctgaccactt cgtccgcatc cgctttcaac aggctgcgca acaaagtttt accggcactg
     5281 ggggcatcgc tggatacggc ataaaatgca ccggcaggag tcaggcaggg atataaattc
     5341 gcttgtagtg aaagtgttga ttccatatta aacctccagt cccggatcaa tagaaaataa
     5401 cattgcgcta accaattgtt ttacgtcatc ttccttacgg gcatcaattt caaaaaccgg
     5461 aacattaaga ttatgttttg caagatattt gtgatacacg tcgataccgg gctgagagcg
     5521 tatatccatc ttggtaatac cgacaacgac ggatgccttc tccagcagcc ctcgaaacga
     5581 atgtaaaaag aattccaaat ctttcaacga gttggttcgg gcattatcta aaagcaatac
     5641 caaacccata ctgccttggc ttaagatttc ccacataaag ttgaaccgtt cctgaccggg
     5701 cgtactatat aaatggactt tggtatcctc atccaagctg atggcccgta gtccatcgcc
     5761 actgtcgtat tccttttcct atccaaagtc atgtcggatg cggaagcatc ggtctggacg
     5821 agtgcttcgt ccgaaatagc cgcaatggca gtggttttcc ctacgccgac aggtcctgtg
     5881 aaaataattt tattttctct catctctccg cctcttagct gcccaacagt tttttcatca
     5941 gtctttgcag aaggccgcgc gactgggatt gggatggtgt cgtgattttt tccgcttttt
     6001 tcatcatttc actatcagaa gcagaatcgg ccccgatatt tattttatcc gccatatcgg
     6061 agtatgcctg ttgtgaaacc gttttcaaat ctaccgacaa aaacccggtt gtataggttg
     6121 ccgcaagata attcagaata tcattgaggt ttaaaggcat cactttatac aacacgttaa
     6181 ggttgacgga tgccttggtc aaaaatgccg acaagcgtat cgaccccggc acatttgcct
     6241 accgggtcag gtttggccaa gatttcaacg taaacggagt atcgggaaaa atcggataaa
     6301 tcaacctgtc ctgcgccgtc caaatggaaa actgccacat acaggacata atgcctactt
     6361 tagccttttc gcgccattgc gagttatcgg gaacagtctt gcaactgacc tgcaaatttt
     6421 cgtctttgca caattcttcg agtttttaca cactttctgt cagcaaaacc cgttgtatcg
     6481 aggggaaaac aattaaggac cggcttattt ccatgcaaga tagcgatgtc ctgcctgttc
     6541 ttttccgcaa accgcaacgc ccccaataat cctttattcg ggttaaactg gcgtatcgta
     6601 accgtacgct gcacattccc gttgtttttc gccgaccggt ctgcgggtgc aataaacgat
     6661 ttcccataaa cattctcgcc ctgcaataac ttgcggagca taggaaacaa tgtttcaaac
     6721 cgaatcggtt tgggcaggta gggaacttca gaatcgggaa ctttctccga acagacggcg
     6781 acgggtatag ccttataacg cccggcaagc tctttccaaa gttcaaaacc gccctcggca
     6841 tcggtatccg ccaaaaccaa atcgggcacg gcactgccgt ctgaagggga tactgtttca
     6901 taacgggtgg tattgtgcat tttgaatgcc attttgaaaa cggattcctg ctgcgccgtc
     6961 atccccgcca acattacgcg tactgtttta attttcggca gttgaacttc catttttatt
     7021 ttccgtaccg ttttattttt ttaatatttt gattcatacg ctgcagcagc cggctcatca
     7081 gcataacgac ctcttcagga agcctgtccg cacgttccct caataccctt aaaaactgcc
     7141 ccaacctatc ccaatcttca gtacgttcat aaatatcgat caacgtaata taaagctggg
     7201 attcgtcggg atatttcaat accgcctgct ccaacacatc cattgccgct tcaatctgac
     7261 catacatcag caacgactct acttctttaa ccgcatcgtc tgccggagac gaaccagtgt
     7321 tgatcaacga agaatcttga agcaccaaat cccgatgttg cgctttgaat ttctgtatat
     7381 ttttcggcag atacccgtgc ctcataccga tgtctttgat ttgccgatcg ttcggcccct
     7441 tttccaaatc atcgaaaact tcatggtaac ccaggctgta cccctaaccc agcatccgct
     7501 ctttaacctg cctgccgtag ttgcccaacg tttggtaaag tttccacaaa tgtccggcaa
     7561 aacggtctat gtccgcgtgt tggtaatcga gtttcaacgc atcgataatc aggtttgcag
     7621 gtttttcgga agtttggatg gcacggtcgt attgtttcga tgccgtttcg tggctgactt
     7681 tgtctttaag gattttcgca ccttgatcgg cgcggaccag gccggcaatc gcaccgattt
     7741 tctcttggct gattccggac acgtcttttt tgccccgcac aatcgggatg cgcttgattt
     7801 cttcggctcc ataagcctta ccgccgacat ccggcggggg cgatgcggaa gctgccgtcg
     7861 cagaaccgcc ttccgcacgt ttttcaatct cctgagtgcc ctatcccaaa ccttcttccg
     7921 ccaagacgcg gatacgcaaa tggtttgaat ctcgctgtaa tgcctgttcg atatattttg
     7981 ccaaaagttc gaaaggaatc agtctgccgt atttttgcag attgtctgcc aaaacatcga
     8041 catcccccac ttcgagattg atatcgatca gctcgcggat aaggttctca ggtttcgctt
     8101 caccatccgg aatgccgtcc agataggcag ccaaagattc ggcagccttg ccctgataac
     8161 cgaattgctt ataaacctga tactccgtaa gcggatcgac ttcttgcgcg gatacggcgg
     8221 cggatgcggt ctcggcactt tcgttccaag accagtcgag ttgcgtgccg tctgaaaccg
     8281 attgaccaag tctgatcgac ccagtccgaa tcattgcttc cgggcggctg cccctgcgta
     8341 ttgccgacac cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc
     8401 aaaagcatca gcaataccgc cagtgccaga ccgataatta atgagttttc caaaggaaac
     8461 ccccgatacc gtaacgagcg gatagctgac cgcccgcaac ggccaattgt ccgcttttat
     8521 tattaacgat ttactgaaat agacttgtaa attttaaatc ataccataat ttaacgtttg
     8581 acaatatgcc ttctgcataa ggctcgccat attacctttt acccacatca gtatcaatac
     8641 ccgatataaa aataactttg cccataagct gccttattgc ctgcccgccg cagagtggcg
     8701 cgcgataaaa aataacggat gataagtcag gcgcactttg cccgacggca taccgaacgc
     8761 cgacaagtaa tcgccaataa atccattgag attttttctt gtccaatttt tttggttcgt
     8821 ggcggtaacg cctgtatatt taaggtgttt gagtacatct aacggcgtat caaagtctag
     8881 tattacctta aaatcctcac accataaaag ctcaaaatct ttggctaacc aagcctgcca
     8941 ttgggataaa gtcgggtaat ttaagcctat atttgtgatt tggcggactt cttttaaatt
     9001 gtctttgcca aaggtcgcaa ccgccaataa tccgtttgtt ttcaagcctg ttttgcaatg
     9061 ggcgataaaa gcgtcgggtt ggtgaaacca ttgcacggca gatgcgcttg cgattaagtc
     9121 aaattgtcgt tgaaaaggaa agttttccgc atcgccgcaa taaaaatcaa aggattgagg
     9181 caatttttca gccagtcgac ttcgtacatc gcacaaatca ttaaataacc aataattcgc
     9241 tgaaatctgt ttttgcagca aggcactcaa cattcctgag ccgcagccca attccaacac
     9301 gttttccaat ggcatatccg gcaaataatc ttgcaaatgc gcgattagat taatcgtcat
     9361 tttttgttgg attaaggcgt gcctgtcata atcgcttaat gctttttgga aagcctgccg
     9421 aatgcgcggt ttatttatgg cagtcagtga ttccatagtg ccgaccaatg ggtaaatctt
     9481 gaaaacaggt aatgcccgcc gtctgtttct tgaacggtgc aacgcggtgt ccaatatcgg
     9541 tgctgattgg caggaataaa aattttatcg cccgatccga acaaggcgtt tgtccagcgg
     9601 ataagatctg tacgcctatc ttgcccgatc atcgcaaaaa gtgcggtaag ttcttgatga
     9661 atttcgtcaa acggacgggc gggaaattgt tggtaatctt caaaagatgc tttatcgcca
     9721 cagattctac gttcaaactt tgaacgagta ttttctgtga gattctccaa tgtgccttta
     9781 aaaacggcgc aagggatacc gaaattatca tcgcaaggca aacctgtgcc attcactgcc
     9841 gttgcggatt ttaatcttat tccttgcaat gccctctctg ccgcccaaac gcccattgac
     9901 cacgccacca aacggatgtg ccgataggcg gaaaaatcaa aatccaaatt taaatcttga
     9961 taatcatagc aaatcaataa atcgtgattt tccggcaaaa tcaaatgatt tacagcatcg
    10021 ggcggcattc cccaacctgc aaaatacagg attaaatgtt cgccttgatg attgtaaaat
    10081 tttgttttca tatcatatcc ttacaggcac gccgcaaact gccgcacttc atccgttgtc
    10141 atatcggcag ttaaagacag gcggattctg gatgtgtttt ttggtactgt cggcggtctg
    10201 atgggcaggc aataataacc ctgaccttgc agatattccg ctttggcaag ggtggcttca
    10261 ttcccgccta aaatataggg gacgatacag gtttcgctcg gcattatttg cgtccgatgc
    10321 gccacttccc gccgtaaaaa tgcgcttaac tgctcaagat gacttctttc ttttgagaat
    10381 tgcggcaatc gttcaaaaat aaaataagtc caagccacat taaacggcgg caatgcggtt
    10441 gaaaaaatca atgggcgcat ttgattaatc aaacattctt tcaatacttg gttgcagacg
    10501 gcatacgccc ccaccgaggc taaggcttta ccgaaagtgc cgaccaataa atcaatctcg
    10561 gcaatcagat tatcccgttc ggcaatcccc aatccgtttt gcccataaac accgattgcg
    10621 tgggcttcat ccacataaag ataagtattg ggaaactgtt tttttaattg gacaagctgt
    10681 ttcaaatccg ccacatcgcc gtccatactg aaaacagatt cggtaacgat aaaagtgcgg
    10741 tcaaattttc cgacgttttt ttcaagcaga tttttcaaat gttcataatc attatgacga
    10801 taacggaaaa acgcacaccg gctcaaacgg atgccgtcaa tcatactggc gtgaacgaat
    10861 ttatctgcca aaatcaaact tttcgtcgtc gtcaaagcag gcaaaatacc gagattggcg
    10921 tgatagccgc tgttgaacaa taacgcgctt tcccgttgga aacgttgtgc gacaagctct
    10981 tccaaatcgg tataaatagg aaagttgccc gttaataaac gtgatgaaga actggtaaaa
    11041 gagggaaaat taccgccgta ttgctgcaaa aaagaccggc gtaagttttc atctgatgcc
    11101 aaccccaaat aatcattaga cgacatattc agcattttgc ggttttcccg cgtaatatac
    11161 cgcccttgat gaatcaaatc cggaatcgaa cgatattgat tttgcgcgcc gagttgttca
    11221 agctgttgtc taaaaacttt cattatgatg taaatattcc tgagttaaag cctgaagcaa
    11281 cccagcatca aacaatcaag taatgcccct ttaatgaaaa tcgttttgcc gccgaccttt
    11341 gccacacccc tgccttcata atccaaggcg gaaatttccg cgatatttgt ttccgttgcc
    11401 attacccaat ccgtccgcac tgtaccgaag cgcatatttt cgcacaaatt ggtacggttt
    11461 caggtatgat ttgccgaaaa cctatcggac acacaccata ttttgatatg aataaaacaa
    11521 tctgccgcac tgccgcacta ttgataagcg gtttttcata cgcaaacacc gtcattcccg
    11581 atgtctcccc cgtcgcacaa ggtcaacatg tcttcattaa catccctcag caacgcctgt
    11641 tcctctacac cgacggcaaa ctgaccaagg tttatcccgt tgcagtaggt cgggcgatga
    11701 cacaaaccaa tctaggcgaa cataaaatcg gggcgaaagc ctataaccct gtttggcaca
    11761 tcccaaaatc catacagaaa gaacgcggcg acggcgtaaa aaccatagcc gccggcccgg
    11821 acaacccttt ggggccggtt tttgtccgct taggagaccc gaaactcggt ttgggcatac
    11881 acggaaccaa tgcaccggcc agcgtcccgg gcatccggag tcacggctgc gtccgcatga
    11941 aatcgcccga cgcgctcgag tttgccaaaa ccatcgccac cggctcgccc gcctccgtca
    12001 tctatcaaat ggcgggcctc aatgaagatg cggatcgcaa cctgtggctt gccgccttcc
    12061 gcgaccctta cggtaagaac aaccttgaca ccgcctccct gaaaaaaagc atcgggcaat
    12121 gggcaaaaac acagggcaaa accatcgcgc ccgagaaagt cgatgccgta ctcaaagacc
    12181 gcaccggatc ggccgtctgt ctgacctgcg gcaaaaacgg caagatgaag atgccgctca
    12241 aatcgctggc gtggatacag ggttcttcct catacagcca agctgaagtc attgaacaaa
    12301 ctgaggaaac caactctgcc gaagtatccg agacacgcac gcccgaagtg cctgatgtac
    12361 acacgcccga ggcacaagcg catttaaata cccaatccga cggcacgccg actgcctata
    12421 ccgaaccggc tgccgattca tcgccgcaag tagaatcacc tgatcaggct gcttccgagc
    12481 cggttgatgt attattttca atagatgtga tacggcaggg gaatttgcgt ttaggtaatt
    12541 gaataacctt ctgattattt aatacttatg tttatcagaa gttgacaggc ggtttggttt
    12601 ttgtttggct tttcctacac cgccgccctg ccgctttctg caacatccaa gcgcacaaat
    12661 atgccgtctg aaggctttag acggcatatt tcacgataat agtcgcccaa tcaaataaac
    12721 acagattgga aatttatttt atttttccct ttaccaatat cccagaactt tccaccaaat
    12781 gctgccgata acggaaaaaa tcagaaaatt gactacgctc atgataaaac ccgccttcca
    12841 ccattctccc attgtggtgt agcccgagcc gaaaatcaca ggtgaagtac cggtcgcata
    12901 atgagtgagg gtcatcataa tgttggatgc ggccgccatc atcagcgcgg tcggcatcgc
    12961 cggggcattc agtgaaacgg cagcagcgag aaatgcgccg aacatagcgg taatatgtgc
    13021 agtagtactg gcaaacatat aatgcgcata catataagca agcacgagga ttacgcccgc
    13081 agccgtgccg ctaacgccca aaccgccgac actttccgcc aacactccgg agaaccattt
    13141 aatcagtccg agtttattta aaaatgcggc catcataatc aatgcgccaa accaaataat
    13201 cgtatcccac gcgctttttt ctttcaaaac atcgtcccaa gtcaatacac cggaaagcaa
    13261 aagcaggctt aatccgataa atgcggtggc ggtggcgttg atactaaaag cgtgattgcc
    13321 ggtaataagg gcgggaacat ctgcccacaa cagcagcaag ataccgaaaa tgaccgccat
    13381 aatgatttcg tctgccgaca ttttacccat ctcgctcaga cggtctttgg caaattgaac
    13441 agcattgggc gtttctttaa tttcaggcgg atacaaaaaa tataaaatca aaggcataac
    13501 gaaaaaggcg ataacgccgg gaacagccat tgcccacgcc cacgcccccc aagaaagacg
    13561 gaaactactg cctaaatttt cggcaatcaa gttgacgatt aaagggttgg gggcagttgc
    13621 agtaataaac atagccgacg aaatgggatt ggaatgataa ttgaccaaag ccaaatattt
    13681 acccatcttg ccttctgtgc cttttgcggg attggagccg taactgccgg caatcgactg
    13741 cataatcgga tgtataatgc cgccgccgcg cgcggtattg gaaggggtaa cgggagccag
    13801 cagcagttcg gaaagagcga gactgtaacc gatgcccagc gtttttcttc caaaaacggc
    13861 gataaacaaa tatccgatac gcatccccag ccctgttttg agcaaaccgc gcgaaatcat
    13921 aactgcgatg gcaatcagcc aaatcaacgg attggcgaac gcactcaacg catcgctcat
    13981 cgccgcgccc ggtttgtcgg cggttacgcc ggttactgcg accaacccga cggcaataat
    14041 cgacagcgcg cccaacggca taaccttgcc gataatggcg gcaatcacac cgacaaacat
    14101 agccagcagc gtccaagcct gaggcttgac cccgtcgggt acgggcagtg ccaaaaccag
    14161 ggcgcacaat actgcggcaa tggcgagggg tatcggtttg aaacccaatt tcatcatatt
    14221 gacctccgta aaaaagaccg tcccgaaaaa tcggaaaaat aatatttaac taattgtttt
    14281 ataagttata ttacgatatt tcaccgtctt tccgatatgc ggctccgggc aacttttgtt
    14341 tcagtatttg aattttcatt agactgaata cgccgtttga acggcacggc gaaaacccgg
    14401 gggatgccgg acgttcagtc ctttttcgca ccttgaaggt aaagcatttc caaggcaatt
    14461 gtggcgccgg ccaaagcggt aatgtcggat tggtcgtaag agggggcaac ttctacaaca
    14521 tccataccga cgatgtcgag atccgtcagc ccacgtagga tttttaatgc cctgtcgctg
    14581 ctcaagccgc cgcatacggg cgtaccggtc ccgggggcga acgacgggtc gagacagtct
    14641 atgtcgaaag tcaggtaaac gggcatattg ccgacggttt ctttgatttt acggacggtc
    14701 tcttcaacac tgtcttcatt gactttgggg gcggacaaca cagtaaaagg caatttttta
    14761 ctgtgttcgg tgcgtatgcc gatttgtacg gaacgggacg ggtcgatgag gccttccttg
    14821 ggggcggtat aaaacatcgt gccgtggtcg tattcgctgc cgttgtcgta ggtgtcggtg
    14881 tgcgcgtcaa aatgaatcag tgcgagtttg ccgaaatagc gggcgtgggc gcgcaacaac
    14941 gggagggtaa tgaaatggtc gccgcccaaa ctcaaacagc gtttgccgaa agaaagtaat
    15001 ttgccggcgt gcgcttccat tttttcgaca aaatccctgc tgtcgccaaa agaaaaaacc
    15061 aagtcgccgc aatcaataat gttcaggcgt tcgcgcacat caaatgtcca cggaaacctg
    15121 cggtgctccc aagcgaggtt gacggaggcg cgccggatgg cttcaggacc gaaacgcgcg
    15181 ccggaacgcc ctgaaaccgc catatcataa ggcacgccgg taataaccca atcggcatgg
    15241 ctttcatacg gcataaaatt aagcggcagg cgcaaaaacc cgaaattatt ggaaacgagg
    15301 gagttgtcgg tttgtcctgc cagtgtgctg tattgcatcg taatgattcc ttgtaattgg
    15361 tttcaatcgg tcgtgatgat tggtgtttga gtaagaaaat cgggcttcag acgacatatc
    15421 cgataccttg atgcgtctat tcgtcttcca aataggtata accattaagc cccgcttcga
    15481 gttcttttaa gaaagacata gcctgcgagg caggaaggtc tgaatgttcg atttgttcgc
    15541 gatagcgttt catcagctct ttcggatctt gataaacgta ttcgagcata tcggcaacgg
    15601 tgtttccttc atcgtaatcg atgacggtaa attgtccgtc ttcccttaca acaacatcag
    15661 cagtggcagt gtcgccgaaa agattgtgca tattgccgag tatttcctga tatgctccca
    15721 ccataaaaaa gcctaaaaac ggcggctctt cttcgggata atcaggcata ggcatcgtac
    15781 cggcgatgcc gtcgccgtcg atgtagtggt caatcgtacc gtctgaatcg caggtaatgt
    15841 ccaacaacac ggcgcggcgc gcaatcggtt cattcaaacc ggtaatagga caaacaggga
    15901 aaagttgatc tatgccccaa gcatcgggca aagattggaa gagtgagaaa ttgacataca
    15961 gcttatcggc aaaacgttct tgcaattcgt caataatggt tcggtgagac cggtgttttt
    16021 cattaaacaa ttcgccgact tcatgacaga tatttaaata cagttgctcc gcccacgcac
    16081 gttgcgccaa actcaacagc ccgacattat actgattatg cacatcggca agatcaaact
    16141 gcccttcgtg tatccagctg cgtaaggaac gtttttcccg cgaggcggaa atatccgtcc
    16201 aagtttccca catactgtgc aacacacgcg gtgcttcggg cgatggcgca tccagccgac
    16261 gcggtttgta acgttcaacg cctataacat tagcaaccaa aacggcgtga tgtgcggtaa
    16321 tgccgcgccc gctctcggta atgattgtcg gatgcggcag cccgtgttcg agacaagcct
    16381 gactgatgcc ccatacgact gtggcggcat attcgttcag gctgtaatta acggaacaat
    16441 ccgattgtgt gcggtttcct tcgtaatcca cgccaagccc gccacctaca tcaaaacagc
    16501 ggatatttac ccccagtttg tgcaactcaa cataaaaccg agccgattcg tgtacacctg
    16561 tggcaacatc acggatgttc ccaagctgcg agcccaaatg gaaatgcaaa agctgcaggc
    16621 agtccagcct gtttttttgt ttcaaaatat cgaccagttg caaaacttgg gaagccgaca
    16681 agccgaattt tgatttttcc ccacccgaag actgccattt tcccgaacct tgggaagcca
    16741 gtctggcgcg cacacccaaa cggggcttga tgccgagttt ttccgcctct tccaatacca
    16801 tttgtatttc ggacagcttc tcaatcacca aataaacctg atgccccagt ttttcgccca
    16861 tcaaggcgaa acggatatat tcacggtctt tatagccgtt gcagacgatt aatgtttgcc
    16921 gggtgccggc gtgtgccaaa accgccatca gttcggcttt agaaccggct tccaaaccat
    16981 gcggttgtcc gcttgacata agcgactcga tgacgcggcg gtgttggttg accttgatag
    17041 ggtaaaccaa acaataaccg cccttatagc cgcactcttc ccgtgccgtc tgaaaggcgc
    17101 ggttaatgtc gcggaggcgg tgttcgagga tttgcggaaa acaaaacaaa acaggcaggc
    17161 gcgcctgatg tttttgttgc acggcttcag tcagtttttg cagtgaaaca gtttgattgt
    17221 gttgcgaggg attggggcgg acgatgattt cgccggaatc gtcaacatca taataaccta
    17281 tgctccaatg attaatgttg cacacttcac ggatggtaag gataggcata ataaacctgc
    17341 tccgtctgtc gtgttgaaaa ggaatgatta taacaaatca gcgtgaaatg tcattttttt
    17401 aataagaaaa gcctgcctca tacctgatga ggaacaggca aaatgccgtc tgaacgcttc
    17461 agacggcatt ttggttcatc tttccatcaa aggagtcagc gatcgagctg ctctttgatg
    17521 attttcaggt cggggtaaga caacacgatg tcgtattcgc ggccgtcttt ataggcttcc
    17581 acttccaaaa caggtttgcc ccagtagtcg tcggcatcga catcgtaaac ctgataaccg
    17641 cgctgttcca acattttcac ggcttttgtg cggttttgtt caaaatgggg atcgccgtaa
    17701 atctgacgct cggcagagtc gccggcaaat gcggttgcgg catttaggga aacaacggcg
    17761 gccaataaca gttttttcat tttcagtcct ttttttatcg gttgattgaa caagatgtgt
    17821 ttttcaatac cgccattaaa acacagcaaa attaggtttg aattagaggg aagtcaagaa
    17881 gcgtaaatgc cgtctgaaaa aacagagccg tcaaacggct ctgttttcct tatgcttctt
    17941 ttgtttcaac tgctttttga cgcagacgca ggctcaattc acgcaactgc ttatcgtcca
    18001 cgctgttggg cgcatcggtc agcaggcatt gggcgcgttg tgttttcggg aaggcaatca
    18061 cgtcgcggat ggattcggca ccggtcatca gcgttaccag acggtcgagg ccgaatgcaa
    18121 ggccgccgtg aggaggcgca ccgaatttca ggttgtccaa gaggaagccg aatttctctt
    18181 gttgctcttc agggctgatt ttcagcgcgg caaacacttt ctcttgtacg tctgcacggt
    18241 ggatacggat agagcctccg ccgatttccc agccgttcaa taccatatcg taggcacggg
    18301 ccaggcagtt tgcaggatcg gaaaccatca ggtcttcatg accttcttta ggagcggtaa
    18361 acggatggtg tacggcaacg tagcggtcgg cttcttcgtc gtattcgaac atcgggaaat
    18421 cgacaaccca caaaggtttc cattcgtctg tgaagtagcc gttgtctttg ccgtgttcca
    18481 agccgacttt gatacgcaat gcgccgacgg cttcgttcac gactttggct ttgtccgcac
    18541 cgaagaagat gatgtcgccg ttttgcgcgg cggtacgctc gatgatttct ttcagggcgt
    18601 tttcagacaa gaattttacg attggagatt gcaggccgct gtcttcgccg ttggagaggt
    18661 tgcccgcatc gtttactttg atgtatgcca gacctttcgc gccgtagatg ccgacaaatt
    18721 tagtgtattc gtcgatttct ttgcggctga atttggcgcc gttcggcacg cgcagagcga
    18781 ctacgcggcc gcctttcatg tcggctgcgc cacggaagac tttgaattct tccgttttca
    18841 tcaggtcggt caactcggtg aattttaagt tgatgcgcat gtccggtttg tcagagccgt
    18901 agtagaacac ggcttcagaa taaggcatgc gtgggaagcc gcccaaatct acgttcaaag
    18961 catctttgaa gacttgtttg gccatgcctt cagtgatgtc catgatttca tcctcgttca
    19021 ggaacgaggt ttccaagtcg atttgagtga attcaggttg gcggtcggcg cgcaagtctt
    19081 cgtcacggaa gcacttggtg atttggtagt aacggtcgaa acccgccacc atcaacagtt
    19141 gtttgaataa ttgcggcgat tgcggcagcg cgaaaaactc gcccggatga acgcggctcg
    19201 gtacgaggta gtcgcgcgcg ccttccggag tggagcgggt cagcatcggc gtttcgatgt
    19261 cgatgaagcc ttgcgcgtcc agataacggc gaacgcccgt agcgacttgg taacgcaggc
    19321 gcaggttgcg ctgcatcaca gggcggcgca ggtcgataac gcggttggtc aggcgcacgt
    19381 tttcgctgat gttttcgtcg tcgatttgga acggcggcgt agcggcggcg ttcaacactt
    19441 cgatttcttt ggcaaggatt tcgattttgc cggaaatcat tttgtcgttg gtcgtgcctt
    19501 cgggacggtt gcgtacgcgg ccggcaatgc ccaaaacgta ttcgttacgg gcggaatcgg
    19561 cagcggcaaa cgcttcgggc gtgtcgggat cgatcacgac ttggacgatg ccttcgcggt
    19621 cgcgcaggtc gataaaaatc acaccgccgt ggtcgcgccg gcggtgtacc cagcctttga
    19681 cggtaacggt ttggcctaag tattgctcgc tgatcaggcc gcaatagttg gtacgcataa
    19741 aatcaccttt tattgattta aactgaaaac agaaaatgcc gtctgaacgg cggctttatt
    19801 gttgttcggg caactccgcc ttttcaggcg gcataggtcc tgccaatgtt ttgacgggca
    19861 ggtcgtcagg gatgaccata cccagcgaaa tcacatattt caacgcttcg tccacgctca
    19921 tatcgagttc gcgcacatcg cttttcttta ccataatata gtaaccgccg gtcgggttgg
    19981 gcgtggtcgg gacatacacg gaaagataat cgccatcctg cggcaatgcg gccttaaccg
    20041 cattcgacac ctgaccggac acgaatgcga ttgtccaaat acccgattgg ggaaacggca
    20101 cgagtaccgg cgttttaaac gaacggctgc tgtcggacag cagcgattcg gatacttttt
    20161 tcacactcga atagatggat ttgacaaccg gaatccgccc caacaggctg tcccacgcgg
    20221 caagaatctg ccggcccaac acgtttgcgg caaataatcc ggttacaaac aatacggcaa
    20281 tggcaacaat aacgccgagc ccggggatat taaaccccaa aacatattgc ggccgccatt
    20341 gcttcggcag caggttgaca agctggtcgg acgcggaaac gatataggaa accacccaaa
    20401 ccgttaccgc aatcggcagc cagaccaaaa tgcctgtaat cagatatttt tttaacgcct
    20461 tggcagcttt gccgccttcg gccgcaggtt ccgtcatctt gcttgattcc gacaaagtcc
    20521 gtacaaaccg cacattatac gcgtttgccc ggattcaaac gaatttttta tcccgccccg
    20581 ccaaaccgcc ggcggcttca gacggcacgg caacttgata tgccgtttga aacggtattc
    20641 agatgccgtc ccagtcgttg aacatcaacc cgataccgat accgttctgc ttgtggttgt
    20701 agtcgatcag gctctcgccg taaccgtgga atccgcgtac cacgcctttg agtttgccct
    20761 taatcggaaa cgtgtaggcg gcttcaatcg cgccgtagcc cgttttgggg ttgtagcgca
    20821 atacggaata cacattctgc ctgtcgttca ggcggtactg cagcttcacg tcgccatacc
    20881 ccatatagtc ggcaatatcg ggattgtcgt ttttatcgcc gctctgatcg aacgcacgca
    20941 cccacacgcg cggaatcacc gtcaatttgc cccattccat gcctgccatg gcataaatcc
    21001 tgttccacga acgcgattcg ggacggctct gtccgttgga ctggtggaca aaacccgcac
    21061 cgagcatacg cagcctgccg ccgaacggca aatccgcctt cacaggctgg gtcaggaaaa
    21121 tttcaggttt gtaatccgta ttgcggaacg gcgcggattt cctgccttgg ttgtaaatct
    21181 gccaatcgga tctttgggtg tagccgaacc acagatccgc gcgggtttta aacaaatttt
    21241 cggcaatttt gcttttgaac gaaacctgca atttggtttc cgcacgtttc tgctgtccga
    21301 atttttcctg tacagtcgta ccgcgcgtcg gcgaactcgg ggcatagttg ggcgaattgt
    21361 tataccaaaa cggcataagg tacatcggat tgtgttcgcg tacgcccaac agcccgcgca
    21421 aatcgttttt gtccaagtcg tacatcaggc tcaaaggcgt atagatatcg gcggtttcgc
    21481 ccgcactgtc ggcaggaagc gcatccccgc ctttttcaac aacaatgacc gcctcgccct
    21541 tatccaagct gctgcggacg gtttccgtca gattgagtac ggctttcgac tcctgccctt
    21601 cctgccctgc cgaagacgga agctgtgccg caaaaatcct gtcgtaacac gccaaacgcg
    21661 taacattgtc cgtcaaagcg gcgcattgca gcgcggtctc tccaaaagcg gatgccgtcg
    21721 gcaacagtcc tgtcaaaaga atatagcgca tatttcgtgt attcatctcc gcccccattg
    21781 tcggcatatt ggttttcaaa cggcatttta tagcggattc ggataaaaaa ccgcaccctt
    21841 tccgccattt cgaactttac ccccgaaata cagaaaaccc cgaaaccgtc gggcttcagg
    21901 gttttccgct tatcgcgtat caaccgcctt ggcggttttg caaaaatcaa gccagtgctt
    21961 ttacttttgc agacagacgg cttttgtggc gagccgcttt gtttttatga aacacgcctt
    22021 tgtcggcgat gcggtcgatg actttgacgg actcttggta aaccgcttgt gcggcagctt
    22081 tatcgcctgc ttcgactgct ttcaatactt ttttcactgc ggtgcggaat gcggtacgca
    22141 ggctagcgtt gtgggcgcgt tgtttgacgg actggcgggc acgtttgcgt gcttgtgcgc
    22201 tgtttgccat attggaatct cctgaaagaa aaataaattc tgtaaacgcg caattttaaa
    22261 gggggtttac attatatgca agcctttttt ctctatccgc ccgtcggaca ccctgacggc
    22321 gcaaaaaaac ggcaaaatcg ggcggccgtt cccccccttt ttggaaacag ggtaattttt
    22381 taggaaaatc aggccaatat cgctttgagt ttgcacaaat ttactttaca atgcccaaac
    22441 cttgcgccgg ggtgtttatg gggcgcgggg ttttcttttt ttccaacctc tttcatcagg
    22501 aatctgcccg tatgaaaaaa tccgtattag ccgttttggc cgcgctgtct ctggcagcct
    22561 gcggcggcag cgaaaaaaac gccgtgcagc ctcaagcagg cagcgcacct gctgccaatg
    22621 ccgaagccgc tgccaccgat actctgaaca tctacaactg gtcgaattat gttgacgaga
    22681 gtacggtcga agactttaaa aaagccaaca atctgaagct gacttacgac ttgtacgaaa
    22741 acaacgaaac gctggaagcc aaaatgctga ccggcaaatc cggttacgat ttggtcgtgc
    22801 cgggcatcgc cttcctgccg cgccaaatcg aggcgggcgc gtatcaaaaa gtcaacaaag
    22861 acctgattcc caactataaa aacatcgatc ccgaactctt gaaaatgctg gaaaccgccg
    22921 acccgggcaa ccagtatgcc gtcccctatt tctccggcgt gaacacggtt gcgattacgg
    22981 cgaagggcaa agagcttttg ggcggcaagc tgcccgaaaa cggctgggat ttgctgttca
    23041 aacccgaata cacccgcaag ctgaaatcct gcggcatcgc cttgtgggac acccccagcg
    23101 aaatgttccc gattttgctg aactacttgg gcaaagaccc caaaggttcg aatcctgaag
    23161 atttgaaggc ggcggcggaa gtgttgaagt ctatccgtcc ggacgtcaaa cgtttcagcc
    23221 cgtccatcat cgacgagctg gcgcgcggcg acatctgcct ggcggcaggc aacggcggcg
    23281 atttgaactt ggcgaaagcg cgttccgaag aagtgaaaaa caacgtcggc atcgaagtgc
    23341 tgacaccgaa aggtatgggc ttctggattg agtcttggct gattcccgcc gatgcgaaaa
    23401 acgtcgccaa cgcccacaaa tacatcaact acacgctcga ccccgaaatc gcggcgaaaa
    23461 acggcatcgc cgtaaccttc gcccccgcca gcaaaccggc gcgcgaaaaa atgcctgccg
    23521 agttggtgaa cacccgttcc atcttcccga acgagcagga tatgaaagac ggtttcgtca
    23581 tgccgcaaat gagcgcggat gcgaaaaaac tgtcggtcag cctgtggcag aaaatcaaag
    23641 tcggcaccaa ctgattcgaa gcattaaaaa tgccgtccga acgatgttcg gacggcattt
    23701 tatattggat ggaaatagaa aatatagtgg attaacaaaa atcaggacaa ggcggcgaag
    23761 ccgcagacag tacaaatagt acggaaccga ttcacttggt gcttcagcac cttagaaaat
    23821 cgttctcttt gagctaaggc gggccaacgc cgtaccggtt tttgttaatc cactatactt
    23881 ataccgttat gcccgcgtcc ggaatttcag attgcggaca tttgtcagga aaaacaaaaa
    23941 accttccgcc gctgcgcagg cgaaaatcct atccgttgga atccggttgt tttaaataaa
    24001 tccttgcagc tttggttttc tgtttttccg ataacaccgt aactttgaaa cgcgtgaagc
    24061 gggaatccga tccgttggga ttttgcaact tcaaatcaat ccgcaaaccg gaatcccgtc
    24121 attcccgcga aagcgggaat ctagaacgca tccgcacgga aacccgcatc ccgtcattcc
    24181 cacggaagtg ggaatctaga atgtcaaatc tcaagaaacc gttttatccg ataagttcct
    24241 atgccgacgg acctggattc ccgcctgcgc gggaatgacg gaggggaacg gcgcacggac
    24301 atttgccgga atcttttatg tttccattat catactcttt tcaatgcgct attccaggaa
    24361 aaagaaaata gcttgtgcag gctgcttttt gtttttcaga cgacctgtaa ttaacggctg
    24421 cttatccaat ctgcttttca aactacctgc aaccgcaaaa tctgctgcaa cccgctccct
    24481 cccctgtggg ggagggtt
//
LOCUS       DAXFZR010000022        24440 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000022 DAXFZR010000000
VERSION     DAXFZR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24440
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.022"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(148..540)
                     /gene="rpsI"
                     /locus_tag="ACFJAS_000776"
     CDS             complement(148..540)
                     /gene="rpsI"
                     /locus_tag="ACFJAS_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC3982072.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(553..984)
                     /gene="rplM"
                     /locus_tag="ACFJAS_000777"
     CDS             complement(553..984)
                     /gene="rplM"
                     /locus_tag="ACFJAS_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC3982073.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1162..1341)
                     /gene="ssrS"
                     /locus_tag="ACFJAS_000778"
     ncRNA           complement(1162..1341)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFJAS_000778"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1347..1652)
                     /locus_tag="ACFJAS_000779"
     CDS             complement(1347..1652)
                     /locus_tag="ACFJAS_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC3982074.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            complement(1663..1992)
                     /locus_tag="ACFJAS_000780"
     CDS             complement(1663..1992)
                     /locus_tag="ACFJAS_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982075.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(2046..3035)
                     /locus_tag="ACFJAS_000781"
     CDS             complement(2046..3035)
                     /locus_tag="ACFJAS_000781"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC3982076.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3158..5860)
                     /gene="ppc"
                     /locus_tag="ACFJAS_000782"
     CDS             complement(3158..5860)
                     /gene="ppc"
                     /locus_tag="ACFJAS_000782"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC3982077.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELGHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            5903..6796
                     /locus_tag="ACFJAS_000783"
     CDS             5903..6796
                     /locus_tag="ACFJAS_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC3982078.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            complement(7196..7420)
                     /locus_tag="ACFJAS_000784"
     CDS             complement(7196..7420)
                     /locus_tag="ACFJAS_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC3982079.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(7421..8809)
                     /locus_tag="ACFJAS_000785"
     CDS             complement(7421..8809)
                     /locus_tag="ACFJAS_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC3982080.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(8895..9716)
                     /gene="prmC"
                     /locus_tag="ACFJAS_000786"
     CDS             complement(8895..9716)
                     /gene="prmC"
                     /locus_tag="ACFJAS_000786"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC3982081.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            complement(10133..10987)
                     /locus_tag="ACFJAS_000787"
     CDS             complement(10133..10987)
                     /locus_tag="ACFJAS_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3982082.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTAADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            complement(11100..11831)
                     /locus_tag="ACFJAS_000788"
     CDS             complement(11100..11831)
                     /locus_tag="ACFJAS_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC3982083.1"
                     /translation="MALLSIRKLHKQYGGVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            complement(11833..12501)
                     /locus_tag="ACFJAS_000789"
     CDS             complement(11833..12501)
                     /locus_tag="ACFJAS_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3982084.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            complement(12491..13150)
                     /locus_tag="ACFJAS_000790"
     CDS             complement(12491..13150)
                     /locus_tag="ACFJAS_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3982085.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFGLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            complement(13294..14778)
                     /gene="tldD"
                     /locus_tag="ACFJAS_000791"
     CDS             complement(13294..14778)
                     /gene="tldD"
                     /locus_tag="ACFJAS_000791"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC3982086.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      14853..14957
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15023..16249
                     /gene="cytX"
                     /locus_tag="ACFJAS_000792"
     CDS             15023..16249
                     /gene="cytX"
                     /locus_tag="ACFJAS_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC3982087.1"
                     /translation="MSGNASSPSSSAAIGLVWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVELSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAEIPVAVGVTLIGTVLAVMLPVTEYKNFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     gene            16246..17346
                     /locus_tag="ACFJAS_000793"
     CDS             16246..17346
                     /locus_tag="ACFJAS_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC3982088.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            17368..17985
                     /gene="thiE"
                     /locus_tag="ACFJAS_000794"
     CDS             17368..17985
                     /gene="thiE"
                     /locus_tag="ACFJAS_000794"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC3982089.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFPTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            18407..18601
                     /gene="thiS"
                     /locus_tag="ACFJAS_000795"
     CDS             18407..18601
                     /gene="thiS"
                     /locus_tag="ACFJAS_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC3982090.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     TETVLHENDKIDIVRPVVGG"
     gene            18680..19468
                     /locus_tag="ACFJAS_000796"
     CDS             18680..19468
                     /locus_tag="ACFJAS_000796"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC3982091.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            19483..19695
                     /locus_tag="ACFJAS_000797"
     CDS             19483..19695
                     /locus_tag="ACFJAS_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFC3982092.1"
                     /translation="MPSEGFRRHRGPKRANRHSPASRLCREEQGNRPENLAARPDTAT
                     NETPGLPGAQAAAQALSTAIEGSFVV"
     gene            complement(20202..21026)
                     /locus_tag="ACFJAS_000798"
     CDS             complement(20202..21026)
                     /locus_tag="ACFJAS_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC3982093.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPVPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(21139..22917)
                     /locus_tag="ACFJAS_000799"
     CDS             complement(21139..22917)
                     /locus_tag="ACFJAS_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC3982094.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRSVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            complement(22914..23420)
                     /gene="rfaE2"
                     /locus_tag="ACFJAS_000800"
     CDS             complement(22914..23420)
                     /gene="rfaE2"
                     /locus_tag="ACFJAS_000800"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC3982095.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            complement(23632..23707)
                     /locus_tag="ACFJAS_000801"
     tRNA            complement(23632..23707)
                     /locus_tag="ACFJAS_000801"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23672..23674),aa:His,seq:gtg)
     gene            complement(23735..23811)
                     /locus_tag="ACFJAS_000802"
     tRNA            complement(23735..23811)
                     /locus_tag="ACFJAS_000802"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23775..23777),aa:Arg,seq:tct)
     gene            complement(23836..23913)
                     /locus_tag="ACFJAS_000803"
     tRNA            complement(23836..23913)
                     /locus_tag="ACFJAS_000803"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23876..23878),aa:Pro,seq:tgg)
     gene            24136..>24440
                     /locus_tag="ACFJAS_000804"
     CDS             24136..>24440
                     /locus_tag="ACFJAS_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HFC3982096.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     "
BASE COUNT         5184 a         7035 c         6784 g         5437 t
ORIGIN      
        1 catccctgac ggtcagttcg tccgtgtagt cgatactggt gctgtttttg atttttgccc
       61 aatccgtaat ctttagatta ccaatgggaa accgcctgtt acaaataaaa aaccctgcga
      121 taagcagggt tttttgaatt tccaaaatca acgtttggag aattgttttg cacggcgtgc
      181 tttgcgcaga cccggttttt tacgttcgac ttcgcgcgca tcgcgggtaa cgaaaccggc
      241 ttgagacaag gcgggtttca aagcagcgtc aaaatcaatc agggcacggg taataccgtg
      301 gcggattgcg ccggactggc cggtttcgcc gccgccgaca acatttactt tgatgtcgag
      361 agattcggcg ttttcagtca gaaccagagg ttggcggaca accatacggc tggtttcgcg
      421 tgcgaagaat tcgtcaacgg gacggccgtt tacgatgatt tgacccgtac ctttagtcag
      481 gaatacacga gccactgaac ttttgcggcg gcctgtgccg tagtagtatt taccgttcat
      541 gtcgtgtcct tattatttca gttccaaaac tttgggttgt tgcgcagcat gggcatgctc
      601 cgcaccagcg tacactttca gttttttaat catggcgtaa cccagcggac ctttgggcag
      661 catacctttt acagcctgtt ccaaagcgcg gcccgggaat tgatcttgca tttcgcggaa
      721 agtgcgctcg tagatgccgc ctggaaaacc ggaatggcgg aagtattttt tatcttcgaa
      781 tttggcaccg gttacacgca gtttgtccgc attgatgacg atgatgtaat cgccggtatc
      841 gacgtggggg gtgtattcag gtttgtgttt gccacgcaga cggctggcga cttcggttgc
      901 aacgcgaccc aagactttgt cttgggcatc gatgacgaac cattcgcgct tcacctcgtg
      961 aggtttcgct gaaaaggttt tcatagtgga aatccagata gatatagaaa gttatggatt
     1021 ttaaagacag gatttgattt tgtcaatcgc attaccgcgt tacggaagga ttttccggat
     1081 ttcggcagac tgcatactgc tttttcgggc ggggcggcgg ccaatgtgaa aaaccgcatc
     1141 gttgcgatgc ggttttgaat gggaatcccc gcgagagccg tttcggccga atccgcttga
     1201 accttgctga caaggcggct gccccgggta gtttcgggtg cgtccgcaaa aggacgctcg
     1261 cgcccactac tgctcccggc aaccttaagc gaacttattg gttcaaagga atatatgcct
     1321 tcgcggacac cgcagggaaa aaggggttat tcctgcccca agcgggatag tgccttttgg
     1381 caggcgttgt ccatatcggt tattttacgc gcaaaatcgc cgattgccaa atcgccgccg
     1441 ttcagggagg ttttcaatag gtcgtggacg acgttgagcg cggccataat gacgattttt
     1501 tcgctgtccg cgacgcggcc gccttcgcgg atggcttcgg ctttgccgtt gagcattccg
     1561 actgcctgca acagtgtgtc tttttcttct gccggcgtgt tgacggtcag ccgggcgtgc
     1621 atgacttcga tgtagacttg ttcgatgttc atcctttaat ccttattgct gcgtttcctg
     1681 ccgttggggg aggcgcgctg ccagtgcgct gattttctcc ctgctctgtt cgagcagggc
     1741 gcggtatcgt gcattttctt ccgtcaggct gtcgattttg ttttgcaggt cttctttgag
     1801 tttgccgact tggacgagca gggcttcgct gagttcgtcg acggcggtct cgtgttcgag
     1861 tttttgccgc tcgtgcgccc gttcgagttc ggcgacggtt tctttgaggc ggcggttttc
     1921 gctgacgagg gtttcgaatt tttgtaccaa cgtataaacg ctgctttcga gtttttcgat
     1981 attttgtttc atgaccttac ctgtctgtat gccgtctggg ggttcagacg gcatctgttt
     2041 gttgtttatt cgaaacgcgc gctgcgttcc atcagccttt cgacgacctg ctgaggggtc
     2101 atttctttgc ggatgagttg cagcagggtt tgggtaatcg gcatatcgat ttggtatttg
     2161 caggcggtat tgaagacttc ttctatggtg ctgacccctt cggaaacgtg cccgatttcg
     2221 accagcacct gatgcagttc cttgccttct gccaaaccca agccgacgcg gcggttgcgc
     2281 gaaagtgcac cggtacaggt caagatgaga tcgccgatgc cggcaagccc catcatggtt
     2341 ttgggctgcg cgcccattgc ggaggcaagg cgggtgattt cggccagtcc gcgcgtaacc
     2401 agtgcggcac gggcgttgag tccgtattcg agtccgtcgg agagtccggt agcgattgcc
     2461 atgacgtttt tgactgaacc gcctactgcc acgccgataa cgtcggtact gccgtaaagc
     2521 ctcatgacgg tcgtgttgag ctgcggtacg agttcttcaa tccactcttg gttttcggag
     2581 gcgaggacga cggcgcaggg cagttgtttg gcgagttcct gtgcaaaact cggaccggaa
     2641 agtacgccga ttttcttatt gtcgggcaat acttctttca agacttggaa ggtcagcagc
     2701 cctgtatcct gctcgaagcc tttgcaggcg gcgaggacgg ggaggtgtcc cgcgccgtac
     2761 tgtttgagca gctctgcgct gcttctcaat ccggcaacgg aggttacgat aaggacaagt
     2821 ccgctgtctt tgagcgcctc tgccaaatcc gcacacactt ccaaggtttc gggaaaggaa
     2881 aagccgggca gtccgcgttt gttttcacgc gcttcctgca tttggcggac ttggtctgcg
     2941 ttgcgcgtcc acagggatac gcggttgccg tgttgggaaa aatgcagggc gagcgccgta
     3001 ccccacgaac ctgcgccgat aacggtaatt ttcattggtc gtctttcaac atatcactgc
     3061 cgttcacttt aaaacaatcg gtgtttctct gcaagtgcgg tcaggtaaat gccgtctgaa
     3121 aggcgttcag acggcatttt gccccgatgc ggcactatca gccggtattg cgcaaacctt
     3181 gcgccacgcc gttgatggtc aggtgcacca tcagcagggc gtgcggattg tcgggttctt
     3241 tgcgcaggcg tttgagcatg gcgacttgca aaccgttgag cgcgttcagg tatggaatcc
     3301 tcaaagcgag cgaacgggcg aggctgcggt tgtcgcgcaa aagttcttcg gtttgcagca
     3361 ggtcgagcag ggctttgcgg ctgcgtcggt attcttcctt aatcatgccg aagatgactt
     3421 ttgccttttc gggtgattcg ctcaaaccgg cgtagttttc cgccaaggtg atgtcggttt
     3481 tcgccatcac ttgttccata ttggagagca tcgcttggaa gaaggggttg ttttgggcgt
     3541 gtccgcgcag ggcggcgagg gtttcggggc tgccttcgca caaggtttcc accgcgctgc
     3601 cgaaaccgta ccaagccggc agcatgaggc ggttctgcat ccaggagaat acccacggaa
     3661 tcgcgcgtaa atcctgaatt cgcgccaggg ttttgcggct ggcgggacgg ctgccgaggt
     3721 tgagggtggc gatttcctga atcgggctgg tttgcaggaa gtagtcgatg aagtcggggt
     3781 gggtaatcag ttcgcggtag tatttgaacg atacgtccga caatgcctgc atcagtttgg
     3841 catcagggtc ttttttatcc ggcaggatgc tggcttccaa agtcgcggca accaaggttt
     3901 ccaagttgcg ttgggcgttg ccggggtcgg cgtatttggc ggtgatgact tcgccttgct
     3961 cggtaatgcg gatttgtccg gcaacgctgc ctgcgggttg cgccaagatg gcttggtaag
     4021 acgggccgcc gccgcgtccg acgctgccgc ctcgaccgtg gaacaggcgc atacggacat
     4081 cgtatttttt gaagagttcg accaagccca attccgcctg atgcagacac catgagctgg
     4141 taacgtagcc gccgtctttg ttggagtcgg aatagccgag catgatttct tggatgttgc
     4201 cgcggctttc gagcagggca tcataccagt cgaggcggaa catggtttcc atgaccggac
     4261 aggcattttc gagcgcttcg atggtttcaa acagaggcac gatgttgatg cggctgtgcg
     4321 gtttgccgtt ttccaccgcc aacaggccgc tttctttcag cagcaatgcc aaggcgagca
     4381 ggtcgccggg ttgttcgcag ttggaaataa tgctttgtgt tacggcatct tcgccgaatt
     4441 cgtctttgat tttgcgcgct tcgttgaaaa tcgccaattc gcggcgggta tggtcgctgt
     4501 atgtgatgaa cgggctgtac agcggacgtt ggtggcccaa ttcgcgcaac aaggcggctt
     4561 gtttttgctc ttcgttcagg ctgttgtagt cttccaagcc tgcgtgttgg aaaagctcgg
     4621 caaccacatc ggcgtgtttg cctgcgtgtt ggcgcaggtc gagcggcatc atgtggaagc
     4681 cgaacacgga cacggaacgg atgaggtctg ccaaacggcc ttcggcaagc aggcggctgc
     4741 cgttgtcgat aagggaacgt tgcaattttt tcaaatcatc gagaaatttt tgggccgaag
     4801 cataaggctc gagaaagccg aatttgcagc ccatgcccaa accgagcgag cgcgctttgc
     4861 ccatagcgcg cgccataatg taggcaatgg cgcggcggta aggttcttcg gtgcgggcga
     4921 tttcttcgtc aggcgagagg gctgccagtg ccattacgtc gtcgttgact ttgacgcggc
     4981 ggatggaaag cggcagttcg cggtaaagtt tgtcgagttc gctgcggtaa aaacggaaca
     5041 cggcatcggc gtggcggcgg aaggcaaagc gcagggtttc gccagaaaca aacggattgc
     5101 cgtcgcggtc gccgccgatc cagccgccga ttttaaggat gttcggaacg cggacatcgg
     5161 gataggccgt ctgaaagtcg tgttccatct tgcggtagag tttgggcagg gcttcaaaaa
     5221 agctcatcgg gaagatggac acgccgttgt tgatttcgtc gttgacgctg agtttgtggc
     5281 ggcgcgtttc gctggtctgc cacaagccca gaagcacggt gtcgatttcg cggcgcagcc
     5341 gtgccagcgc gtcggcattg gtgcagcgtt cgcgttgcgg cagcagcgcg cggatgcggc
     5401 ggttgaaatt caaaacggtt tggcgttgca cttcggtcgg gtgcgcggtc aaaacggcgg
     5461 taacggacgt attgtccaac tgccgctgca ccgatttgcc gtcggctttc cccgctttga
     5521 gcctgcggac ggtttccgtc aggctgcctt ctgctgcgtt gtggccggca tcttcgtgga
     5581 tttggcggcg gcgttcgtgg tgcacgtctt cggcgatatt cagaatctgg gcgaacagcc
     5641 cgcaggcaag cgtcagatcg taggtctgcc gttcgtccaa ttgcggcaat actttttcaa
     5701 tcaatgccgc gctgtcgtcg gaagtggaca agagtttgac cgtttcgaca accaacggcg
     5761 aggcttcttc gtgcaggagg ttgaacaggg actgtttcaa aaattccgcg tccgccgcca
     5821 aagccgcgtc cttcggattg ttcaggatat gcagttgcat gattttcctc tcattgccgt
     5881 aaatactgta aatgtacctc aaatgccgca tccgtgccaa accgttcaca ctttaaccac
     5941 tcatgtcccg aaatgccgtc tgaagttgaa cgccgcccga cggcggcgtt acaatcgccc
     6001 gcaactgttt tttccgaaca tcatcatgac cgcgaccgaa cacgacaacg acgacgcact
     6061 cctgctgcgg tacagccgcc acatcctctt ggacgaaatc ggcatcgaag ggcagcagaa
     6121 gctttccgcc gcgcatattt tggtcgtcgg ctgcggcgga ttgggcgccg ccgccctgcc
     6181 ctatctcgcc gcctcgggcg tcggcacgct gaccatagcc gattccgaca cggtcgagct
     6241 gcacaacctg caacgccaag tcgcatttga cgagggcgat gtcggcaagc ctaaagccga
     6301 aaccttggca ggccgcctga aacgcatcaa ccatactgtc gatgtccgcg ccgtcaacga
     6361 aaaactcgac ggctgccgcc tgaccggttt ggttcaaacc gccgacatcg ttttagactg
     6421 ctgcgacaac tacgccacgc ggcaggcggt caaccgtgcc tgcgtgcaaa cgaaaacacc
     6481 gctggtttca ggggcggcgg tacgttttga aggacagctt gccgcatacc gtcccgactt
     6541 gcccgactcg ccgtgttacg cctgcctgtt cgacggcgga tcggcttcag acggcatctg
     6601 ttccctcttc ggcgtgctct cgccgctggt cggcatcgtc ggctgcacac aggcggcgga
     6661 ggctctgaaa atcctgctgg aagcgggcga accgtcgcac ggcaggctgg cggtttaccg
     6721 tgccttggaa gggggctggc aatatttcga cctgccgcgc aaccccgaat gcccggtttg
     6781 cggcgcggca cggtaaaacc ctgccgccgt ttcagacggc atccgaacgg atgcggcgga
     6841 acggttttaa aaatttaaaa atttacattt ctttgcaaaa aaataaactt accttataat
     6901 tgcagttgtt ttagcaatgt ctgtttcgca gactcattga gtaagacgtt ttccccgtaa
     6961 tgtgtttggc cgtctgtccc ctttgggttc ggacggcttt ttttggctgt gtttgaatac
     7021 ccggttggtt ttatctgttt gcagcggggg aagccgctca tcgccgttcg gacaagaacc
     7081 ggtttcatcg gataaaaggc attttgtccg actgattaag gttacatctt agggttgcac
     7141 cggcggaata ttcaaacaca gcctttattt aaggaaatcc ggatacggcg gcgcatcaat
     7201 aatgcggcgg aatctcgtcg cgcagggaat acggctcttg cgcgtcggga ttcctgtcct
     7261 gcattttttg atacagcagc ctcaactgag cctgctgcaa atccaacgtc tgccgcaatt
     7321 ccgccaccat cgcgttcaga ccggagatta cgtcctcctg aagcgcggtt tggatttcca
     7381 gttcgacaat acggcgttcc aactcttgaa ccgcgtccat ttacagcacc atcgcggcaa
     7441 tccagccggc aatcagaagc gggatgttgt agtggatgaa ggtcgggatg acggaatcgc
     7501 ggatatggtc gtgccgcccg tcggcgttca gccccatcgt cgggcccagc gtggaatcgg
     7561 acgcgggcga accggcatcg cccaacgccc ccgccgtgcc gacaatggcg gcggtggcaa
     7621 gcggcgaaaa gcccaaaccg gtacacagcg gcacataaat cgcggcaata atcggcaaag
     7681 tggaaaagga cgaaccgatg cccatcgtta ccaaaagccc caccaccagc atcgccaacg
     7741 ccgccatacc tttgctgttg ccgaatatcg ccatactgct ttccaccagc ggctgaatat
     7801 gcccggtcgc attcatcacg gcggcaaaac cctgcgcggc aatcataatg aagccgacca
     7861 tcgccatcat cttgatgcct tcgccgaata cgtcgtttgc cttgtcgcgg ttaatgaccc
     7921 ccaacatcat aaatacggcg aaaccgagca tcgcgcccaa caccagcgaa tcttcataca
     7981 tcaactgtat ggcaaagcat acggcgatgg cggcggcggc aaccaggctg cggtaggcgg
     8041 acggctgcgg acggtttgcc gcatcggcgt tgcccgccgt atcggcattg ttgctttggt
     8101 acagtcgcgg tttgcggtag tggacaaacg ccagcaggag tccggccagc attcccaatg
     8161 cgggaatcgc cattgccgcc atcacgttga tgtttttcac atcaagctgc ggcgcggcgg
     8221 aatggatgtt gcccaacagg atttcgttca aaaaaatcgc gccgaagccg taaggcagga
     8281 acatataagt cgtaaccagc ccgaaagtga tgacgcacgc aatcaggcgg cggtcgattt
     8341 tcaggcggtt aaacaccaaa agcagcggcg gaacaatcat cgggataaag gcgatgtgga
     8401 tgggtatgac gttctgactc ataatgccca tcacaaggat gatggaaagc agcagccatt
     8461 tgaccgcgcc ctcgcccgaa cgcacgctgt cgggcatacc gccccggttc agcttgcgga
     8521 cgaccgcgcc ggcaagctgc tgcggcaggc cggaatgggt aatcgccatc gcaaacgcac
     8581 caagcatcgc ataagaaagc gcaatcttcg cgccgccttc caaacctttg ttgaacacgg
     8641 ggataatccc cgcctgactg acctgtcccg ccgcatcggc aatgttttgc agcggcatac
     8701 ccgccaccgc gccgccgaca aacgcgccga ccgtcaggct caataccacg tgcacgcgcg
     8761 acagcgacag caccagcata acgattacgg caactacgac tgcattcatt gtttatcgct
     8821 ccaaacctat aaatgtttac atatcgaaac acatcataac ccaataacgg aaaatccgcc
     8881 aattttgcaa acaattattt caaatgcttc atatacttcc ccagcgtaac cctgtccaaa
     8941 ccggccaaat ccggcaggat ttccactccc gaaaaaccat tctccgccaa cacgccgcgc
     9001 accgccgcgc cctgatcgaa accgtgttcc aacaacaaaa aaccgccttc cgccaaacgg
     9061 tcgggcgcgc cttgcgccaa ggtgcggatg cagcttaggc cgtctgaaaa gtcggtcagc
     9121 gcgatttgcg gctcaaaccg caaatcgcct tgcgacaaat gtttatcgcc gttttcgata
     9181 tagggcgggt tggacacgat gatgtcccat tgcctttcag acggcatatc ggtgtcgaac
     9241 cacgaaccgt gtgcaaattc gacccgcgcg cccaaatccg ccgcattttt ccgcgccgtt
     9301 tcaagggcgg gcgtgctgat gtcggatgcg cgcacaaacg catcggggcg ttcgagcgcg
     9361 acggttacgg caactgcgcc gctgcccgtc cccaaatccc acacgcgccc gttttcgggc
     9421 aggcgcgcca atacggcttc gaccaagtgt tcggtttcgg ggcgcggaat cagcacgttc
     9481 gggttgactg taaagcgtct gccataaaat tcgcgcacac ctaaaatata ggcaaccggc
     9541 tcgccgttca gacggcgttg cgccagcctg tccgcccgct gtcggatttc gtccggcatt
     9601 tcttccccgc cccgcgtcaa caactgcacg cgcgtatatt ccgaaacata ttgcagcagc
     9661 atccttgctt caattttagg cagttttgac aagcccaacc attcgtcaaa cgtcattttt
     9721 cgtcccgtct gccgccgatg cggcttgaac attttttccg gctttgcggc acgcttgttc
     9781 aaaaaatatc attttgcatt gaaattatat aataaaggaa aacaccttat cgccgtttca
     9841 ataccgtagg aatttatccg aataacagaa acgctccgcc gtcatcccgc gaaaacggtc
     9901 atccggaaac ataaggctga agcgttttat ctggaatgac gatttggaag tttcccgaaa
     9961 ttcaaaaaca ggcaaaaccg gacgaacttg atttccgcac gcgcaagaat aataaaataa
    10021 ataaaacgat gagatagttg atttttcctt tgaatttgca atattttcgg cggctgaaaa
    10081 aaaatccgca cccgccttca aatgcggata cggattttct tgtcaaacaa acttattcgg
    10141 ccaacaatgc ttccggtttg actttctcgc catacacggg caagagggtt ttttcatagg
    10201 cggctttcag acggccgtct tttttcatgg cggcgatttc gccgttgacc cagttcagga
    10261 ggtcggcgtt gcctttttga acggcggggg cgataaattc ggcaggaccg agattgccga
    10321 tggcgacttc aaagttcggg ttttctttcg cccacgccca cagcagggcg ttgtcgtgcg
    10381 cgagtgctac gccgcgaccg tctttcagcg cgtcgaaggt ttcagtgttt tggtcgaatt
    10441 tcaacagctt gacttcggga tggcttttgg tgaagaaagc gtcggcggcg gtgcctttgt
    10501 tgaccagcag ggtttggtct ttcaattgcg ccatgtcggt aatcggtttg tttttggggg
    10561 aaaccacgcc caacgccact ttcatgtaag gatcggcgaa atcgacggct tcggcgcgtt
    10621 cgggggtttg ggtaaagttg gcgaggatga ggtcgacttt gccggaacgg acgtattcga
    10681 cgcggtttgc ggcctcggtc aggacgaatt cgactttgtc ggggctgccg agcaggtctt
    10741 tggccaggtc tttggcgatt tcaacgtcaa agccttggtt tttgccgttt gcgtcaacat
    10801 agccgaacgg gggtttgtcg ccgaatacgc cgatgcggat aacgcctttt tctttgatgg
    10861 cggcaacggt tgccgcaccg ctgctttgtg aagattgggc atcgccggag ccgcccccgc
    10921 aggcggtcag accgacggcg atggcggcgg aagccaaaag ggctttgagt ttggcgttca
    10981 atttcattgg aaatctcctt tttgaatgtt tgcttgggat gtttcaacac acaggacgac
    11041 acataaagca ctgccctatg aaatgccgtc tgaaaagggt tcggacggtt tgcggtaaat
    11101 cagtagtcca tacctgccag aaattggcgg gcgcgttcgc tttttggtgc ggaaaaaaag
    11161 gtttcggggt cggacgattc gacgatgccg cctttgtcca taaagacgat gcggtcggca
    11221 actttgcgtg cgaaccccat ttcgtgggtt acgatgagca tactcatccc ttcgcgggcg
    11281 agttccaaaa ccacttccaa gacttcgcgc accatttcgg ggtcaagtgc ggcggtgatt
    11341 tcgtccagca ggatgacttc cggattcagg cacagggcgc ggacaatggc gatgcgctgt
    11401 ttctgaccgc cggaaagttc gcgcggatag gcgtttttgc ggtctagcag tccgacgcgt
    11461 tccaacagtt tgccggcttg cgcctctgct tcggcacggt cgcggttttg tacctttacc
    11521 gggcctaaga ggatgttttc gatgacggtc atgtgggcaa acagttcgta gctttgaaag
    11581 accataccga ctttttgccg ggcggtttgc caggaaacgt ctttgccgaa ttcgccgaca
    11641 ccgtccatca cgatgctgcc gccttggtgc ggctccaaac cgttgacgca gcgcaggagg
    11701 gtggatttgc cgcagccgga cgggcccagc agtacgatga cttcgccttt ttccaagtcc
    11761 aagtctaagg attggatggc ggttacgccg ccgtattgtt tgtgcagctt gcggatgctc
    11821 agtaaagcca tgtcagtgtt cccatttttg ttcaagtttt gccgccagta gagatagcgg
    11881 ccagcaacag aagaaataca gcataaaaat caggccgtaa acccaaaatg aagcattggg
    11941 ctgcgtcagc aacgagtttt caataatttg ctgcccgact ttgacgactt cgatcacgcc
    12001 gataagccag gcgagcgagc tggttttgat catgcgcgtg aacaggttga ccgcgccggg
    12061 caatacgcgg cggatgcttt gcggcagctc gatgcagcgg aatacctgcc cccggctcaa
    12121 gccgggcgca agacccgatt cgacctggtg tttttcaatc gattccagtg cgccgcgcac
    12181 caagtcgccc atttcggcaa cgccccacag ggaaaatacc cagacgcaga cccaaaatcc
    12241 gccgatgtgg atgcctgtcc agacggatag gccgaaatac aggccgaaca gccacaccaa
    12301 aatcggcacg attcggatgg tttcgagata aaaccgtccg acaaagcgga caagccggtt
    12361 gcgcgaacgc aaaaccaagc cgaacagcgt gcccaatacg caagaagccg caacagagat
    12421 taaagaaatt tgtgccgtta agagcaggcc ttcgcccaac cgtgccgcat tttttccttc
    12481 aaacagccat tcaaacgcca tatttcgcac tccgtacgcg gttttcaatc cgccgcgcca
    12541 acaaagaaac aggcagcagg ataatcaaat aagcggcaaa cagcaggaac aaggcttcat
    12601 tggttttgta gtccataccg atgacgtctt tggtaacaaa taacaattcc gcaatgccga
    12661 ccgtgctgac gacggatgtt tctttcatta aaaacaatat gtttgcgccg atggcgggaa
    12721 cggcgaccgc ccaaacctgc ggcaattcga catagcggaa cacttgaaag cggctcaaac
    12781 cgattgcctt gcccgctccg acctgccctt tggggacggc gaggatgccg gcgcggacag
    12841 cttccgccat atagcttgcg cccaagaaaa ccagcgcgat tacgccgcag gtgaaaccgt
    12901 cccatttgat gcccattttc ggcagaccgt aatataggaa aaacaattgg atcagcaggg
    12961 gcgtattgcg cgacagctcg atataggcgc gcgccaatgc gtagaagggg cggatgcggt
    13021 atgccgttac cacggcgacg ggcaggccga acagcaacga caaaacaacg ccgtaaacgg
    13081 acaattccag cgtcagcttt gccgcatcgg cgaatttggg tacggcatcg attaaataag
    13141 gccagttcat cagaaatcag cttaacgaaa ggcggtattt taatgaactt gtaagataat
    13201 gttaaagaat gcttaggaat atcaaacatc ggaaaaaaga ataaggcgat gccgtctgaa
    13261 aacctttcag acggcatcgt gccggattcc gcgtcagatt gcgctgccgc cgacggtcag
    13321 cccggcatcg atacgcaggg tcggctgccc cacgccgacg gggacgctct gcccttcttt
    13381 gccgcacacg ccgacaccgc tgtcgagcgc ggtatcgttg cctatcatcg aaacgtgttt
    13441 cagcacttcg gggccgttgc cgataatggt cgcgcctttg acggggtatt gcagcctgcc
    13501 gccttccacc caccacgctt cggacgcgcc gaagacaaat ttcccgctgg tgatgtccac
    13561 ctgtccgccg ccgaagttga cggcgtaaat gcccttgtcg atggacgcga tgatttcttc
    13621 cggctcgtag ctgccgtttt ccataaaggt gttggtcata cgcggcatcg ggacggaggc
    13681 gtagctttgg cggcggccgt tgccggtcga ttgcgtaccc gtcaggcggg cgttggtttc
    13741 gtcctgcata tagccgacca agatgccgtc ttcaatcaat acggtgcggc gtgtttcgtt
    13801 gccttcgtcg tcgatgttga gcgaaccgcg ccggccggca atatcgccct gatcgacgac
    13861 ggtaacgcct ttggcggcga cgcgttcgcc gatccggccg gaaaagacgc tggtcccctt
    13921 gcggttgaaa tcgccttcca aaccgtgtcc gaccgcttcg tgcagcaaca cgcccggcca
    13981 accgttgccc aaaacgacgg tcatctcgcc ggcaggcgca ggacgggatt cgagattggt
    14041 cagggcttgt ttgacggcgg catcgacaaa ctgccgcacc aaggtttcat cgaaataagc
    14101 caagtcgtag cgtcctccgc cgcccgcgcc gccctgttcg cggcgttcgc cctgtttggc
    14161 gatgacggta acgttcaggc gcaccatcgg gcggatgtcg gcggcgtgtt tgccgtccag
    14221 acgggcgagg taaaccatat cgtattcgca ggtcaaaccg gccatcactt gcacgatgcg
    14281 cggatcggcg gctttggcga ttgcttccac tttgttcaac agcgcgactt tggcggcgga
    14341 atcgaggccg gcaatggggt cggatgcgga acaaaccggc ttgccgcgcg tttcagacgg
    14401 cattttggcg gacaccttgc cgcctgccgc cccaatcgcg cggacggcgc gggcggaacg
    14461 gtttatcgaa tcgatgcaca ggctgtcggc gtaggcaaag gcggttttgt cgcccgaaac
    14521 ggcgcgcacg cccacaccct gatcgatttg gaagctgccc gatttgacga tgccctcttc
    14581 caaatgccag ctttcataag cggtgcgctg gcagtagata tcggcataat cgacgtggtg
    14641 cgcaccgatg atgcacaggc ttttggcgag cagttcgggg gaaaggcggt tggcttcgag
    14701 cagccgcgcc tgtacggcgg aataggtcgg atgcatagtg tcggcgcata aaaaatcagg
    14761 ggcttgatta tacggcattt gttatggaaa tgcgccgtgc cgtcgaaaat gtaagaattt
    14821 tgccaacgca cgttgctttg tgtaaactta aagccttctt gtcggagtgc cgcccgccgg
    14881 gcggctgaga ttgcgaaagc aaaatccgta gaacctgtcg gggtaatgcc tgcgtaggaa
    14941 acaaaacctc cccgcccttt cgggcttccg ttcccttttc cgcacttccc cgaccacctt
    15001 ccttcttttt aaaggacgtc atatgtcggg caatgcctcc tctccttcat cttccgccgc
    15061 catcgggctg gtttggttcg gcgcggcggt atcgattgcc gaaatcagca cgggtacgct
    15121 gctcgccccc ttgggctggc agcgcggtct ggcggccctg cttttgggtc atgccgtcgg
    15181 cggcgcgctg ttttttgcgg cggcgtatat cggcgcactg accggacgca gctcgatgga
    15241 aagtgtgcgc ctgtcgttcg gcaaatgcgg ttcagtgctg ttttccgtgg cgaatatgct
    15301 gcaactggcc ggctggacgg cggtgatgat ttacgtcggc gcaacggtca gctccgcttt
    15361 gggcaaagtg ttgtgggacg gcgaatcctt tgtctggtgg gcattggcaa acggcgcact
    15421 gatcgtgctg tggctggttt tcggcgcacg cagaacgggc gggctgaaaa ccgtttcgat
    15481 gctgctgatg ctgcttgccg tgttgtggtt gagcgtcgaa gtgttcgctt cgtccggcac
    15541 aaacgccgcg cccgccgttt cagacggcat gaccttcgga acggcagtcg aactgtccgc
    15601 cgtcatgccg ctttcctggc tgccgctggc cgccgactac acgcgccaag cacgccgccc
    15661 gtttgcggca accctgacgg caacgctcgc ctatacgctg acgggctgct ggatgtatgc
    15721 cttgggtttg gcggcggctc tgtttaccgg agaaaccgac gtggcgaaaa tcctgttggg
    15781 cgcgggcttg ggcataacgg gcattctggc agtcgtcctc tccaccgtta ccacaacgtt
    15841 tctcgatacc tattccgccg gcgcgagtgc gaacaacatt tccgcgcgtt ttgcggaaat
    15901 acccgtcgct gtcggcgtta ccctgatcgg cacggtgctt gccgtcatgc tgcccgttac
    15961 cgaatataaa aacttcctgc tgcttatcgg ctcggtattt gcgccgatgg cggcggtttt
    16021 gattgccgac tttttcgtct taaaacggcg tgaggagatt gaaggctttg actttgccgg
    16081 actggttctg tggctggcag gcttcatcct ctaccgcttc ctgctctcgt ccggttggga
    16141 aagcagcatc ggtctgaccg cccccgtaat gtctgccgtt gccattgcca ccgtatcggt
    16201 acgccttttc tttaaaaaaa cccaatcttt acaaaggaac ccgtcatgac ccgtatcgcc
    16261 gtcctcggag gcggcctttc cggaaggctg accgcattgc agcttgcaga acaaggttat
    16321 cagattgaac ttttcgacaa gggcacccgc caaggcgaac acgccgccgc ctatgttgcc
    16381 gccgcgatgc tcgcgcctgc ggcggaagcg gtcgaggcaa cgcccgaagt catcaggctg
    16441 ggcaggcaga gcattccgct ttggcgcggc atccgatgcc gtctgaacac gcacacgatg
    16501 atgcaggaaa acggcagcct gattgtgtgg cacgggcagg acaagccatt atccagcgag
    16561 ttcgtccgcc atctcaaacg cggcggcgta gcggatgacg aaatcgtccg ttggcgcgcc
    16621 gatgaaatcg ccgaacgcga accgcaactc ggcggacgtt tttcagacgg catctacctg
    16681 ccgaccgaag gccagctcga cgggcggcaa atattgtctg cacttgccga cgctttggac
    16741 gaactgaacg tcccttgcca ttgggaacac gaatgcgccc cccaagacct gcaagcccaa
    16801 tacgactggg taatcgactg ccggggctac ggcgcgaaaa ccgcgtggaa ccaatccccc
    16861 gagcacacca gcaccttgcg cggcatacgc ggcgaagtgg cgcgggttta cacgcccgaa
    16921 atcacgctca accgccccgt gcgcctgctg cacccgcgct atccgctcta catcgccccg
    16981 aaagaaaacc acgtcttcgt catcggcgcg acccaaatcg aaagcgaaag ccaagccccc
    17041 gccagcgtac gttccgggct ggaactctta tccgcgctct atgccgtcca ccccgccttc
    17101 ggcgaagccg acatcctcga aatcgccgcc ggcctgcgcc ccacgctcaa ccaccacaac
    17161 cccgaaatcc gctacagccg cgaacgccgc ctcatcgaaa tcaacggcct tttccggcac
    17221 ggctttatga tttcccccgc cgtaaccgcc gccgccgtca gattggcagt ggcactgttt
    17281 gacggaaaag acgcgcccga acgtgatgaa gaaagcggtt tggcgtatat cggaagacaa
    17341 gattaaagcc gcccgaaagg acaccctatg accttcccgc ccctaaaatc cctacttaaa
    17401 ttctacgccg tcgttcccac cgccgattgg gtggggcgca tggtcaaagc aggtgccgac
    17461 acggtgcaac tgcgctgtaa aaccttgcac ggcaacgaat tgaaacgcga aatcgcccgc
    17521 tgcgtcgccg cctgtcaagg cagccgcaca cagcttttca tcaacgacca ctggcgcgag
    17581 gcaatcgaag cgggcgcata cggcgtacat ctcgggcagg aagacatgga caccgccgac
    17641 cttgccgcca tcgccgccgc cggtttgcgt ttgggtttga gtacgcactc cgtcgccgaa
    17701 ctcgaccgcg ccctgtccgt acaccccggc tacatcgcca gcggcgcgat tttcccgacc
    17761 acgaccaaac aaatgcccac cgccccgcaa ggcttggaca aattgcgcga atatgtggaa
    17821 caggctcgcg gcacgcccgt cgtcgccatc ggcggcatcg atttgaacaa cgcccgagcc
    17881 gtgctggcca ccggagtttc ctcactcgcc gccgtccgcg ccgtaaccga agcggcaaat
    17941 cccgaagcgg tggttaaagc gtttcaggct ttgtgggatg gataaaaccg aaagaagaaa
    18001 attcaattgc cgtgtaggca aaacttagcc cgttatcgca aatatgctta actttaaatg
    18061 tggcatatca tcaaattccg tcattcccgc tcaggcggga atccgccgta aaacttgaga
    18121 aaccatcatt tgaaaaacag tttccgaatt tcaaaaatgg attcccgcct gcgcgggaat
    18181 gacggcaacc ggccggttgc gtatcaaaaa ataaagtaat tcggctagat atagtggatt
    18241 aacaaaaatc aggacaaggc ggcgaagccg cagacagtac aaatagtacg gaaccggttc
    18301 gcccggtgct tgggcacctt agggaaccgt tccctttgag ccggggcggg gcaacgccgt
    18361 accggttttt gttaatccgc tataaataca gagacatcga gaaaccatga acatcatctt
    18421 aaacggcgga cccgccgaac ttcacggcac aagcgttgcc gacctcatcg cccaaaccgc
    18481 gccgcaaaaa ccctttgccg tggcggtcaa caccgttttc atccccaaag gcgcgtatac
    18541 ggaaaccgtt ttacacgaaa acgacaaaat cgacatcgtc cgccccgtcg tcggcggctg
    18601 aatgccgtct gaaaccccaa aggcacgccg ccggctttca gacggcatcg cgtccgacaa
    18661 ccataccaaa gaatccatca tgctcaccct gtacggcgaa actttccctt cgcggctgct
    18721 gctcggcacg gccgcctacc cgacccctga aatcctcaaa caatccgtcc gaaccgcccg
    18781 gcccgcgatg attaccgtct cgctgcgccg cacgggatgc ggcggcgagg cgcacggtca
    18841 ggggttttgg tcgctgcttc aagaaaccgg cgttcccgtc ctgccgaaca cggcaggctg
    18901 ccaaagcgtg caggaagcgg taacgacggc gcaaatggcg cgcgaagtgt ttgaaaccga
    18961 ttggataaaa ttggaactca tcggcgacga cgacaccttg cagccggacg tgttccaact
    19021 cgtcgaagcg gcggaaatcc tgattaaaga cggcttcaaa gtgctgcctt attgcaccga
    19081 agacctgatt gcctgccgcc gcctgctcga tgcgggctgt caggcgttga tgccgtgggc
    19141 ggctcccatc ggcacgggtt tgggggcggt tcacgcctat gcgctcaaaa tcctgcgcga
    19201 acgcctgccc gacacgccgc tgattatcga cgcgggcttg ggtttgcctt cccaagcggc
    19261 acaagtgatg gaatggggtt ttgacggcgt attgttaaac accgccgttt cccgcagcgg
    19321 cgaccccgtc aacatggcgc gcgccttcgc actcgccgtc gaatccggac ggctggcatt
    19381 tgaagccggg ccggtcgaag cgcgaaccaa agcccaagcc agcacgccga cagtcggaca
    19441 accgttttgg cattcggcgg aatattgaaa aaggcggcaa aaatgccgtc tgaaggcttc
    19501 agacggcatc gcggccccaa acgggcaaac cgccattccc cggcatcacg gctttgtcgt
    19561 gaggaacagg gaaaccggcc ggaaaacctt gccgcccgcc ccgacaccgc aaccaacgaa
    19621 acacccggac tccccggcgc gcaggctgct gcgcaagccc taagtacggc aatagaaggg
    19681 agttttgtcg tttgaataca tttaaaaata cctcggggca tccgtaagcc ttcatccgca
    19741 acggtttcac cgcttttgca tccccgaatc cgcgctcaaa cacccccgaa tggcaaccct
    19801 gtccgcacca aatcggacgg ttgttcaaac acgggcaacc ttatttccgt caggcacgaa
    19861 gcccccgtcc atgcctgccg accccgattt gtccggcgca atgaaagttt gccgacccaa
    19921 atcacaaaca tcggcggaca gattaatttg tttatttttc atcgcattac aaaaaatccg
    19981 catttatttt taaattttta ttgataatta ttattattag cgtataatca aaaccactcg
    20041 gaagccgtcc gttccgaacc attaaacact gtattccctc acaattcact ttcacacttg
    20101 gagtcggcat atacgagaca tacattccct ttttaatcag atactcaaaa ccgaaacgcc
    20161 aaacccaccc tagcggtggg tttggcgttt ttggtctgca cttatttgca agacttgagg
    20221 ttcagtttgc cgtataggga cgtgatttta cggatttcgt ccgcatcggc ggcattcacg
    20281 cccgtaaaca aaaccgtcat acgcgacacg ctcaaagaat cgtcctgcct gtcggcttcg
    20341 gcaaagtgtt cgacaatatg cgccccgccg aatccggcat cggcaagccg gttttgcagg
    20401 gcttgggcgt tttcccggct gttgccgaca cccaaactca atgctccgtc aaacggtacg
    20461 gggttgaaac ctttggcaga cagctccgcc gcctgatttt cggcatcggc ggaaacgggc
    20521 aggacgacgc ggtaggtttt gtcggcaggt ttggctttgg cggtgcgttt ttcgacgctc
    20581 ctgctggcaa cgtgcgacca tttgcccaaa agccctttga tgcggtggta gtcgtcttcg
    20641 tccatcgtga ggcttgcctg cgcggcgcat aaggcatccg ccgcgccgtt ttcacgttcc
    20701 gcctgcgcct tttcggcggc gagtttttcg cggcgggctt tttcttcacg ctgttttttc
    20761 tccctcagtt ttttctgttc cgcttctttt ttcaagcgca actgctccgc ctgttcttcg
    20821 ctcaggatgt cgcccggttt gagcagtgcc gccgtatcct ccacggatgc cgccgcattg
    20881 ccggcggcgg gcgttgcggg gggcgtgttt tcgacggccc ggttttccgg cacgccgccc
    20941 gccgctttaa cggccaattt atagcccacc gtgccgccga atacggcaat attgagtacc
    21001 gccaaaaggg tgaagagcca tttcattaga atttcctcaa tgtttgaaac cccgcccgcc
    21061 cgggcggcag tctgtcgggc ggcagaatcc ggacagattc ggacggatgc cgccccgtgt
    21121 tgaaaaaagg catatttttt aagcgtgttc cgattccccg ccttcggcgg caatcaggtt
    21181 cagcagcccg tggatgacga ggttgtccgc cacgcgcacg gtattttccg ccaaaaatgc
    21241 aggcggcagg gcttcggcga ctttcgccgc gccgccgccg gtaatgatga catcgacagg
    21301 cttgcccgcc ccggtttttt ctttcagacg cccgtgcatc atcataaccg agccgcaaac
    21361 cgcgtccatc atgccgcttg cgacggcgtt gcccgttgtg gtcgggaaag ggtaacgttt
    21421 gccggcgggg cggttgaggt tggcggttcg gacggcgagt gattctttca tcaggtggaa
    21481 gccgggcatg atggttccgc cgagataatg tccgtcatcg gtgagcgcgt caaccgttac
    21541 cgccgtgccg caactgacga cgacgcaggc gttgcggctg aagcggcggc tgcccaaggc
    21601 gttgaaccaa cggtcggaac cgtgttcttc ggggtggcgg tagtggttgc gtatgcccaa
    21661 agcctgtgcg gaagacggca gccactcgat ttttcgggcg agctgttcct tcacttgtgc
    21721 ctttttggat tctccgcaca cggcgcaacc gacgatgcgg acatttccat ccgccttttc
    21781 cgcccactcc gcgcccaaag gcgacaaatc gcggtacggc gcgctgccga cggttgcgaa
    21841 cgtgccgttt tccacccacg cccacttgag ccggctgttc ccgccgtcca acagcaaaaa
    21901 acgttccgaa tcccgccgct tcggcacgga aaccgacctg tcgtcgggcc gcaggctgat
    21961 ttcgccgctg acgaccgtct gttcgccctc tgccgtttcc aagtgcagaa cgcctcgtcc
    22021 gtccacgcct ttaaccgtgc cttcgcacac ggtttcgccg tcgcgcaaca gcaataccgc
    22081 cttgccgtgg tcgcggttgg ccgtttcata ctcatttaaa aatggcgcga acccttcttc
    22141 cgcatattgt tccaacaccg cgcccagttc cgcaagcaat gtttccagca atacggcggc
    22201 atcggcattg ccccgccgcg atgccgtctg aaacagcgac tgcacggaag cggcgttttc
    22261 cacttccttg ggcagcacga aattgatgcc gataccgacc acggcaaccg ttttaccgcc
    22321 cgccctgact gtttcaatca gaatgccgcc caatttgtcg cgtccgacga ccaaatcgtt
    22381 tggccacttg atttgcgttt ccaaacccaa acaccccaaa gcgcgccggc acgcaagtgc
    22441 cgcaacaggc gacagcgaac ccaactcata ctgcggccgg tcaaacgccc agccgaaact
    22501 gaacatcagg cactcgccca aacggtgcga ccacttccgc ccctgccgcc ccctgccctt
    22561 actttgcagg tgggtcacgc atatggtttt gtgcgccttg tccggcgcaa tccgcgccaa
    22621 ttccagtatc tcgtcgttgc tggacgcgca ctcgtgcttc aatgccgtct gaaaacccga
    22681 cctttccccc agatcgcgca aaccttcggc atcgaaaacc gccaaggggc gcaccagccg
    22741 ccaatagccg tcgtgttggc gcaacagccc gcgtatatgc gccggcatct gctgccaaaa
    22801 accgttgagc tgctgcggct tcatgtccgc ctcacgcgcc aattgcgata cgtgttgcgg
    22861 caaaccgtcg gcaagctccg ccaacacccg ccagtgcgaa ggcttcaaaa ccgtcatttt
    22921 ccgccctctg ccgcacggat ttttgccaaa gtcttcgttg tcgaagtctg atgcagaaac
    22981 ggaattgaaa acacctgacc gccgcgtgcc agcgtttccg ccgcaccgac aatcttatcc
    23041 acgacccaat cgccgccctt gaccaacacc tccggcttga ccgcctcaat caacgccgcc
    23101 ggcgtatcct cgtcaaacca agtaaccaaa tccacacttt ccaacgcggc ggcaacggcg
    23161 gcacggttct ccaaaggatt aaccgggcgg tcgccgccct tgcccaaacg ccgcaccgaa
    23221 gcatcggtat tcaacgccag caccaacgcc gcccctgccg aacgcgcctg cgccagataa
    23281 gtaacgtgcc ccctgtggag gatgtcgaaa cagccgttgg taaacaccag cgggcgcggc
    23341 aacaacgcca aacgcgccgc caacgcctcg ggcggacaga ttttcgattc aaaatcaggg
    23401 acagaccaag cgtcaaccat caaagcctcc gacaaaaacc ataaaagaca gaaaaaccca
    23461 catgatacag aagcatatgc gaaaggcaaa gccggcggcg caaatgccgt atgaagcggt
    23521 ttccgtatga ccggcccggc tttcagacgg cataccgccg caccggtacg acggcgcgga
    23581 cagtacgcgc aaacaggaaa agacccgtac cgaaaagtac gggcctttat ctggggtggc
    23641 tgatggggct cgaacccacg acaaccggaa tcacaatccg gggctctacc aactgagcta
    23701 cagccaccat aaaaacggtt ttcaatcaaa ttcttggcac gcccgacagg aatcgaacct
    23761 gtaacccccg acttagaagg tcggtgctct atccggttga gctacgggcg ctcatgccga
    23821 ttcgtgctga ttgattggtc ggggcggtgg gattcgaact cacgaccctc tgctcccaaa
    23881 gcagatgcgc taaccgggct gcgctacgcc ccgacttgaa gaagcggaat atacagttca
    23941 gggaaagatg cgtcaacatt tattttcaaa acaccaagat gaaaaatata gttttttgat
    24001 ttgaaaaaat atttaatccg tccaaacagc cgtattttat ttcggggcaa atttattttc
    24061 ggcattctgc tgtaaaaaca aacggaaaat gcgataattt tcagcatttt ctacctgttt
    24121 aacaaaagga cggatatgtc ggcacaactg atcaacggta aagaagtttc gcaaaaacac
    24181 ctgcaggcga ttgccgaagc ggtggcgcaa cgccaacagg acaatctgca cacgccttgc
    24241 ctggccgtgg ttttggtcgg aggcgaccct gccggcgcag tttatgtccg caacaagaaa
    24301 accgcctgcc aaaaatgcgg catcaagtcg ctgtcgtacg aactgcccga atcaacatcg
    24361 caggaagaac tgctggcatt ggtcgaccgt ctgaatgccg attccgaagt ggacggtatt
    24421 ctggttcagc ttccactgcc
//
LOCUS       DAXFZR010000023        24251 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000023 DAXFZR010000000
VERSION     DAXFZR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24251
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.023"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..494
                     /locus_tag="ACFJAS_000805"
     CDS             <1..494
                     /locus_tag="ACFJAS_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3982097.1"
                     /translation="SKYSVSIKELLRNENKNSGNHLNIKTQKTEHQENGTFHAASSLG
                     LSTIYDFDTGSRFKPYAGVRVAYGHVRHQVRSVQQETEIVTTYPESGAPSSVPGAAVG
                     KPAYHESRSISSLGFGAVAGVGIDITPKLTLDAGYRYHNWGRLENTRFKTHEASLGVR
                     YRF"
     gene            complement(468..677)
                     /locus_tag="ACFJAS_000806"
     CDS             complement(468..677)
                     /locus_tag="ACFJAS_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982098.1"
                     /translation="MPSERLSDGIFPCILNRVYETPDAFGGNVGCCAMFGGDQRTKCA
                     GKDLMPSEGSDGIGIGESEAVAHAQ"
     gene            complement(695..1252)
                     /locus_tag="ACFJAS_000807"
     CDS             complement(695..1252)
                     /locus_tag="ACFJAS_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC3982099.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFVPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1327..2961)
                     /locus_tag="ACFJAS_000808"
     CDS             complement(1327..2961)
                     /locus_tag="ACFJAS_000808"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC3982100.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYIRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3024..3362)
                     /locus_tag="ACFJAS_000809"
     CDS             complement(3024..3362)
                     /locus_tag="ACFJAS_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982101.1"
                     /translation="MIFKCRPNAFQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVML
                     FVPDCPVGGAAGFFQYEMLCPLFRQGRLQTGSNFAYDVFMSAGRLNGGRKPPRRLLVF
                     LPCFAPLPYN"
     gene            complement(3359..4465)
                     /gene="serC"
                     /locus_tag="ACFJAS_000810"
     CDS             complement(3359..4465)
                     /gene="serC"
                     /locus_tag="ACFJAS_000810"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC3982102.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4831..5262
                     /gene="rimP"
                     /locus_tag="ACFJAS_000811"
     CDS             4831..5262
                     /gene="rimP"
                     /locus_tag="ACFJAS_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC3982103.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5290..6792
                     /gene="nusA"
                     /locus_tag="ACFJAS_000812"
     CDS             5290..6792
                     /gene="nusA"
                     /locus_tag="ACFJAS_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC3982104.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6804..9635
                     /gene="infB"
                     /locus_tag="ACFJAS_000813"
     CDS             6804..9635
                     /gene="infB"
                     /locus_tag="ACFJAS_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC3982105.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9795..11135)
                     /locus_tag="ACFJAS_000814"
     CDS             complement(9795..11135)
                     /locus_tag="ACFJAS_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC3982106.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(11209..12549)
                     /locus_tag="ACFJAS_000815"
     CDS             complement(11209..12549)
                     /locus_tag="ACFJAS_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC3982107.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRDRMLRDTLERVRAGSFWLWVVVASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12692..13318
                     /locus_tag="ACFJAS_000816"
     CDS             12692..13318
                     /locus_tag="ACFJAS_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC3982108.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13383..14801)
                     /locus_tag="ACFJAS_000817"
     CDS             complement(13383..14801)
                     /locus_tag="ACFJAS_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC3982109.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     MQKKAGQDLSYQYEFHEFHDKQ"
     gene            15128..15856
                     /locus_tag="ACFJAS_000818"
     CDS             15128..15856
                     /locus_tag="ACFJAS_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC3982110.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRTQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15915..16403)
                     /locus_tag="ACFJAS_000819"
     CDS             complement(15915..16403)
                     /locus_tag="ACFJAS_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC3982111.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17425..17883)
                     /locus_tag="ACFJAS_000820"
     CDS             complement(17425..17883)
                     /locus_tag="ACFJAS_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC3982112.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLD"
     gene            18105..19163
                     /gene="alr"
                     /locus_tag="ACFJAS_000821"
     CDS             18105..19163
                     /gene="alr"
                     /locus_tag="ACFJAS_000821"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC3982113.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(19705..21060)
                     /locus_tag="ACFJAS_000822"
     CDS             complement(19705..21060)
                     /locus_tag="ACFJAS_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC3982114.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(21075..21347)
                     /locus_tag="ACFJAS_000823"
     CDS             complement(21075..21347)
                     /locus_tag="ACFJAS_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC3982115.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(21472..22209)
                     /locus_tag="ACFJAS_000824"
     CDS             complement(21472..22209)
                     /locus_tag="ACFJAS_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC3982116.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(22347..23246)
                     /gene="prmB"
                     /locus_tag="ACFJAS_000825"
     CDS             complement(22347..23246)
                     /gene="prmB"
                     /locus_tag="ACFJAS_000825"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC3982117.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            23390..23797
                     /locus_tag="ACFJAS_000826"
     CDS             23390..23797
                     /locus_tag="ACFJAS_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982118.1"
                     /translation="MADGMGNLIGWEETGLVEGKQWITAKDDKVSDVCNANGGMGVIG
                     LHEPFSHGALTIPEHPNGRCAVVPVVGRTGKLAKGSRAMETGDGRQVRLFPYRAANWG
                     NLLKQRSFVKRLCSMRVITLSAKAMSMKAAGMN"
     gene            23731..24063
                     /locus_tag="ACFJAS_000827"
     CDS             23731..24063
                     /locus_tag="ACFJAS_000827"
                     /inference="COORDINATES: protein motif:HMM:NF037911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982119.1"
                     /translation="MQYARDNFVGKSYVNESSGHELKVTWQGVKHAASKSNSIELSLM
                     VALDKVLMNAKYEGSFPNKLGRDHIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHY
                     DHFILKDK"
BASE COUNT         6429 a         6612 c         5963 g         5247 t
ORIGIN      
        1 acagtaaata ttccgtgagc ataaaagagt tgctaagaaa cgagaataaa aattctggca
       61 accatcttaa cataaaaacc caaaagacgg aacatcagga aaacggcaca ttccacgccg
      121 cctcttctct cggcttgtca accatttacg atttcgatac cggttcccgc ttcaaaccct
      181 atgcaggcgt gcgcgtcgcc tacggacacg tcagacatca ggttcgttcg gttcaacaag
      241 aaactgagat tgttaccact tacccagaaa gcggtgcgcc aagttctgtc ccaggagctg
      301 cggtcggaaa acctgcctat cacgaaagcc gcagcatcag cagcttgggc ttcggcgcag
      361 tggcaggcgt aggcatcgac atcacgccca agctgaccct ggacgccgga taccgctacc
      421 acaactgggg acgcttggaa aacacccgct tcaaaaccca cgaagcctca ttgggcgtgc
      481 gctaccgctt ctgattcccc gataccgatg ccgtctgaac cttcagacgg catgaggtct
      541 ttgcctgcgc acttggtgcg ctggtcgcct ccgaacatgg cgcaacaccc gacatttccg
      601 ccgaacgcat cgggcgtttc ataaacccgg tttaaaatgc atggaaaaat gccgtctgaa
      661 agcctttcag acggcatttt gtttgagatt ccgtttacca atggctgaca aacgcttcca
      721 aatcggtatt cttgggctta tgcacttcct ctgtcggcgt gccgaccatc atcagcccga
      781 tgattttatc cttatcctcg caaccgaaag cctcccgcaa caatgggctg ttgacccaca
      841 tccccgtaat ccagacattg tcgaatccct gagccgttgc cgccagttgc agcgcatacg
      901 ccgcacaacc cgccgtcagc atctgctccc attccggttt cggcttaggc acatcgcggt
      961 tcggcacaaa cgttaccccg atgaccatcg gcgccatatt gcccactttt tccgcctttt
     1021 tcatcgcatc gtcgccgaaa ttcagttcgg caaccgtttg cttcaacaca tcgcgaaaac
     1081 gttgcaatcc tacctcgcct tgaatcacgg taaaacggaa ggggcgcatg ttgccgtgat
     1141 cggaaacttg ggttgccgcc tgcaatattt gttccaactc cgccgcatcg ggggcggggt
     1201 gcttcagctt tttggaagat cggcggttcg tcaataattt taaagcatcc atatcgttat
     1261 actccggtca tcggtcgggt gtttcggaca catatgccgt gaaggcttca gacggcatat
     1321 ccggcatcag cgcggacggc ggcaggctgc caatatatcc atttccttcc gataggtttg
     1381 gctgttggaa atgtccatca gccccaatac cgtgctgaaa tagtggtcgt gcgaatattc
     1441 gttttccgcc gctttttgtt tgaggcattg gaaatctatg cccccgtgtt ggcggaaggc
     1501 tttggaaaac cacataacca tcgggatatg cgtctgtccg gaaggcgcga tggcgtaagg
     1561 cgcggcgtgc aggtacatcc cgttttcgcc caaactttcg ccgtggtcgg aaacataata
     1621 caccgcgctt tccaaatcgt cgcggttttc aagtttgcgg ataaccttgt cgataaactg
     1681 atccacatac aaaaccgtat tgtcgtaagt gttgaccaac gtggcgcggg cgcatttgtc
     1741 gatttcgttg gtatcgcatg tcggcgtgaa tttgcgctcg gcttcggtat agcgttcgta
     1801 atacgtcggc ccgtggctgc cgatggtatg caggattaaa accgcgtctt tatcgttttt
     1861 gttgagggct tcgtcgaact tggtcaacag gatattgtcg aggcactcgc cgttgcggca
     1921 gtattcgggc aggttgagcg aggtaacgtc ggtattcggc actttgccgc acacgccctt
     1981 gcagccggaa tcgttttcca accaagtaac ttccacgccg gcgcgctgta cgatgtccag
     2041 caggttgtct tggtgttcgg ctttgatttc gtcataatcc gtgcggtcga aggttgagaa
     2101 catacacggc agggagtgcg cggtcgatgt gccgcagctt ctgacctgcg ggaaattgac
     2161 aatttcatcg ccgcgcgcgg caagcagcgg cgtagtttgg cggatgtaac cgttcaaacc
     2221 ccagttggcg gcacgcgtag tctcgcccac gaccagcacc acgaaacggc gcaggctgcc
     2281 ggccggccgg ttttgcacga ccgccatatc caattgcgta taaggaatat tggaacgctt
     2341 ccaatctttg tatttcgaca cgcccgcgcc gatgaaatta gacggcacaa tcagatgggt
     2401 tactgattta ttgttgcgga aaaacgaggc gtaatcctga tattgcaaca tcgcgatgcc
     2461 caacgcgcac aaaaaggaaa cggcggcaag cacaaggcgc gtcaaaagct ccttatacca
     2521 aacgcggtat ttgaccttga cggcgatata tgccagcgcg ggcaatacgc ccaaacatac
     2581 aatccacagc acatagcccg gcgtaatcag gcgcgcgctt tccgccgccg tggtttgcaa
     2641 gacattattc aacatcgact tgttgaaata gatattgaaa aatatttctt ggtaagacac
     2701 cgccgcactg ataaccaata tcaatggaat caatacttta tgcacaaaag gcagggcaat
     2761 gacgtgaaaa acgaaattac ttaaaaaaaa cagcaccacc ggcatcgtat agaggaagat
     2821 gtccgccccg gtgtcgttaa aagggcgaag ttcgacaact ttggcaaaaa aggcgtaatt
     2881 caataccagc gaagaataca gggaaagaaa ggcaatcagc gcggaagagc cgagcttcgg
     2941 cctcaggttc ggttttatca tttggaatgt gtcggataag ggttggaaaa ggcatccgga
     3001 atccggatta ttgaaaaaga ttcttaatta taaggcaacg gagcaaagca gggcaagaaa
     3061 acgagtaggc ggcgcggggg tttccgcccg ccattcaaac gtccggcaga cataaaaaca
     3121 tcgtaagcaa aattcgaacc ggtctgcaac cgcccctgcc ggaaaagcgg gcaaagcatt
     3181 tcatattgga aaaacccggc cgcgccgccg acgggacagt ccggcacaaa cagcatcacg
     3241 cccggattga aaaggacgga tagccgccgg cagcacaatc ttaccacctc caaaacgccg
     3301 ccggagaacg ccgaaacagc cgatgccgtc tgaaacgcat tcgggcggca tttgaaaatc
     3361 aaccgtaacg ccgttggaaa tcgcgcataa aatctgccaa agcccgcacg ccttcaagcg
     3421 gcatcgcatt ataaatgctg gcgcgcatcc cgccgacaga cttatagccc ttcaacaggc
     3481 acaagccctg caactcggct tccaacacaa aacggcggtc aagctcctca tcccccgttt
     3541 ggaacacgac attcatttta gaacgcgcat tcggacggat acggttgata tagaaaccgc
     3601 cgctgccatc tatcgtctca tacaaggttt gcgccttcag ccgattgacc gcttcaattt
     3661 ttttcacacc gccctgcgcc tgcagccagc ggaacaccag ccccgacata taaatcgcgt
     3721 aagttgacgg cgtgttgtac ataccgtcgc ggttcaaatg agaacggtag ttgaacacat
     3781 cgggaatatc gttcggacaa cgctcgagca aatcctcacg cacaatcacc accgtaactc
     3841 ctgccggccc gatgtttttc tgtgcgcctg cgtaaatcag tccgtagtcg gcaacatcaa
     3901 actcacgcga cagaatctcg ctggacatat cgcacaccaa cggcggcatg ccgtctgaaa
     3961 ggcgcggcac ttcacggtat tgcaggccgt tgacagtttc attgacggca aaatggacaa
     4021 acgccgaatc gggtgcaaca tcccacgttt ccacaggcgg caggtcaaga tagtcgaact
     4081 gctcgccccc gtgcgccgcc aaacggattt ccgtatcggt caaacggctc atctgttcat
     4141 aggcgatacg gctccagttg cccgttacca ccgcgtcggc agtgcggaaa ccgtgtgcca
     4201 gattcatggc tgccatatta aattgggttg ttgctccgcc ctgaagaaac aatatcttat
     4261 agttgtcagg cactttcaaa agctgcctca aatcctgttc cgcatgatgc aggatgctca
     4321 aaaacatttc cgaacggtgg ctcatttcca tcacggaaaa acccgtaccg ttgtagtccg
     4381 acatttcctg ctgcgccgtc ctcaacacag cttcaggcaa cacggcaggg ccggcggaaa
     4441 aattgtaaat cggataaaga gacatgatgc agccctggtt ctgaacaata acccgcccga
     4501 ttttaggctt gcagcaggct ttgtgcaagg atgggaaata cctgccctcc gcccgattcc
     4561 atgccgcccg aacacggaaa ataatatcaa tatattgatt tacaaacata aaaatcatgc
     4621 tagcgacaaa tagatacatt tgttttgtca acaatattca cgatttccca ttacaaacct
     4681 cccgtacacc cgcttttttc cgtcccaaaa acacaaaata aatcaacact ttcatttctc
     4741 cgcaaaagcg gttataatca cgccgatttt tcaacttttg acgaaaaatg gccacggggc
     4801 cattttttta ttatgtacat agggagcagc atggatattc aaaccatcct cgaaaaaacc
     4861 ctgcccggcc tgggatacga actggtcgat ttcgaactgg ccgcgcaagg aacattgcgc
     4921 gtgttcatcg acaaagaagg cggcattacc gtcgaagact gcgccaccgt cagcaaccac
     4981 ttgagccgcg tcttcatggt tgaagacatc ggctacaaaa acctggaaat ttccagcccc
     5041 ggactcgacc gccccttgaa aaaagccgcc gacttcgtgc gctttgccgg tcagaatgcc
     5101 aaaatcaaaa cccgcctgcc gataggcggt cagaaaaact ttatcggtaa aatcgaaggc
     5161 tgcgaaaacg acactgttac cgtttccttc gacggcaaaa ccgtgcaaat cgaattgggc
     5221 aacatcgaca aagcccgcct gcgccccgaa ttcaaattct aaaacacagc aatattggag
     5281 atgttcaaaa tgagtcgtga aatgttacag ctggcagaag cactggcaag cgaaaaaaac
     5341 gttgatgcgg aagtcgtctt ccaagcactg gaattcgcac tctctaccgc cgccaagaaa
     5401 aaggcagacc gcgaacacat ggacgtgcgc gtccaaatca accgcgatac cggcgaatac
     5461 caaaccttcc gccgctggct gattgtggcc gatgaagact atacctatcc cgatgtcgaa
     5521 aaaaccatcg aggaaatcca agaggaaatc cccgatacga ccatccaaat cggcgaatac
     5581 tacgaagagc agctgcccaa cgaaggcttc ggccgccaag cggcgcaaac tgccaaacaa
     5641 atcatcctgc aacgcatccg cgatgccgag cgcgagcaga atctgaacga gttcctcgcc
     5701 gtcaaagaag acatcgtgtc cggcacggtc aaacgcgtcg aacgccacgg catcatcgtc
     5761 gaagtcgttg ccggcaaact ggacgcgctg attccgcgcg accaaatgat tccgcgcgaa
     5821 aacttccgca gcggcgaccg catccgcgcc ctcttcctgc gcgtcgaaga aatcggcaac
     5881 accggccgca aacaagtcat cctgagccgt acttccggcg atttcctcgt caaactgtac
     5941 gccaatgaag tacctgaaat tgcaggcggc atgcttgaaa tccgcgctgt cgcccgcgac
     6001 ccgggccagc gtgccaaagt cgccgtcaaa gccaacgacc agcgcatcga tccgcaaggc
     6061 acctgtatcg gcgttcgcgg ttcgcgtgtc aatgccgtca gcaatgaatt gtccggcgag
     6121 cgcatcgacg ttgtcctttg gtcgcccgag cctgcccaat tcgtcatgag cgcgctctca
     6181 cccgccgaag tcagccgcat cgtcatcgac gaagacaaac acgccgtcga tgtcatcgtt
     6241 gccgaggacc ggctcgcgct cgccatcgga cgcggcggtc aaaacgtgcg ccttgcttcc
     6301 gacctgaccg gctggcagct caacatcatg acttccgccg aagcagacga acgcaatgcg
     6361 gcagaagatg ccgccatccg ccgcctgttt atgaatcact tgaatgtaga tgaagaaacc
     6421 gccgacgtac tggttcaaga aggttttgca accttggaag aagtcgccta tgttcctgcc
     6481 gccgaactgc ttgccattga aggatttgac gaagaaatcg tcgatatgct ccgcaaccgt
     6541 gcccgcgatg ccatcctgac catggcgatt gctgccgaag aaaaactggg cgaagtgtcc
     6601 gacgatatgc gtaacctcga aggcgtagat gccgatatgc tcctcagcct tgccgaagca
     6661 ggcattacca cccgcgacga cttggcagag cttgccgtgg acgaactgat tgaaatcacc
     6721 ggtgtaaacg aagaaactgc aaaagccgtc atcctgaccg cacgcgaaca ctggtttacc
     6781 gaagacaaat aaagggggta cagatgagta acacaaccgt agaacaattt gccgccgagc
     6841 tgaaacgccc cgtcgaagac ctgttgaaac agttgaaaga agccggcgtc agcaaaaaca
     6901 gcggcagcga ttccctgacg ctggacgaca aacagcttct gaacgcctac ctgaccaaga
     6961 aaaacggcag caacggcggc accatcagca tccgccgcac caaaaccgaa gtcagcaccg
     7021 ttgacggcgt aaaagtcgaa acacgcaaac gcggacgcac tgtcaacatt ccttctgccg
     7081 aagaattggc agcacaagta aaagccgccc aaacccaagc cgcacctgtt cagccggagc
     7141 agacggcaga agacgcggta aaagcccgag ccgaagctgc cgcacgcgca gaagcccgtg
     7201 ccaaggcaga agcggaagcg gcaaaactga aagcggcaaa agcaggcaac aaagccaaac
     7261 ctgccgcgca gaaacctacc gaagcaaaag ccgaaaccgc acccgttgcg gcggaaacca
     7321 aacccgccga gcccaaagaa aaagccgtca agccgaaaca cgagcgaaac ggcaaaggca
     7381 aagatgccaa aaaaccggcg aaacctgccg cacctgccgt gccgcaaccc gtggtcagcg
     7441 cggaagaaca ggcgcaacgc gacgaagagg cgcgccgcgc cgccgcattg cgcgcccacc
     7501 aggaagccct gttgaaagag aaacaggaac gccaggcacg tcgcgaagcc atgaaacaac
     7561 aggcagaaca acaggcaaaa gccgcacagg aagccaaaac cggcagacag cgtcccgcca
     7621 aacctgccga gaaaccgcag gcagccgcac cggccgtcga aaacaaacct gtcaatccgg
     7681 caaaagcgaa aaaagaagac cgccgcaacc gcgatgacga aggtcaaggc cgcaacgcca
     7741 aaggcaaagg tgcaaaaggc gggcgtgacc gcaacaatgc ccgcaacggc ggcgacgagc
     7801 gcgtacgcgg cggcaagaaa ggcaaaaaac tcaaactcga gccgaaccaa cacgccttcc
     7861 aagcaccgac cgaacccgtc gtacacgagg ttttggttcc cgaaaccatt accgttgccg
     7921 atttggcgca caaaatggcg gtcaaaggcg tggaagtggt caaagccctg atgaagatgg
     7981 gcatgatggt taccatcaac caatccatcg accaagacac cgccctgatt gtcgtcgaag
     8041 aactcggaca catcggcaaa cccgccgccg ccgacgatcc cgaagccttc ttgggcgagg
     8101 gcgcggaagc ggtggaagcc gaagcattgc cgcgtccgcc cgtcgttacc gtcatgggcc
     8161 acgtcgacca cggcaaaacc tcgctgctgg actacatccg ccgcgccaaa gtggtacagg
     8221 gcgaagcggg cggcattacg cagcacatcg gcgcgtacca cgtcaaaacc ccgcgcggcg
     8281 tgattacctt cttggacacc ccgggccacg aagcctttac cgctatgcgc gcgcgcggcg
     8341 cgaaagcaac cgatatcgtg attcttgtgg tcgccgccga cgacggcgtg atgccgcaaa
     8401 ccatcgaggc aattgcccac gctaaagcgg caggcgttcc gattgtggtt gccgtcaata
     8461 aaatcgataa agacactgcc aaccccgaac gcatccgtca ggaactgacc caacacgaag
     8521 tcatccccga cgattggggc ggcacggttc aattcatcga cgtttccgcg aaaaaaggaa
     8581 cgaacatcga cgcgctgctc gaagccgtat tgctcgaagc cgaagtattg gaactgaccg
     8641 cacctgtcga tgcacccgcc aaaggcatca tcgtcgaggc gcgtttggac aaagggcgcg
     8701 gcgcggttgc cacattgctg gttcaaaacg gtacgctgaa aaaaggcgac atgctgctgg
     8761 ccggtacggc attcggcaaa atccgcgcga tggtcgatga aaacggcaaa tccattaccg
     8821 aagccggccc gtccatcccc gtcgaaatcc tcggcttgtc cgacgtaccg aatgcgggtg
     8881 aagacgcgat ggtattggcg gacgagaaaa aagcgcgcga aatcgccctc ttccgccaag
     8941 gcaaataccg cgacgtgcgc cttgccaaac agcaggcggc gaagctggaa aatatgttca
     9001 acaatatggg cgaaacccag gcccaatctt tgtcggtcat catcaaggca gacgttcagg
     9061 gctcttacga ggctttggcg ggcagcctga aaaaactgtc cgccgacgag gtgaaagtga
     9121 acgtgttgca cagcggcgtg ggcggcatta ccgaatcgga tgtcaacctt gccatcgctt
     9181 cgggcgcgtt cattatcggc tttaacgtgc gtgcagatgc ctcttcgcgc aaacttgccg
     9241 aaaatgaaaa cgtggaaatc cgctactaca acatcatcta cgatgccatc gacgacgtga
     9301 aggcggcgat gagcggcatg ctttctcccg aaaagaaaga acaggttacc ggtacggtcg
     9361 aaatccgtca ggtcatctcc gtttccaagg tcggcaacat tgcaggctgt atggtcaccg
     9421 acggcgtggt caaacgcgat tcccatatcc gcctcatccg caacaacgtg gtcatccaca
     9481 cgggcgaact ggcttcgttg aaacgctata aagacgacgt aaaagaagtc cgcatgggct
     9541 tcgagtgcgg tctgatgctc aaaggctaca acgaaatcat ggaaggcgac caactggaat
     9601 gcttcgacat cgtcgaagtt gcccgcaccc tgtaattcct ttgcaaataa aatgccgtct
     9661 gaagcgttca gacggcaacg aaacgggttc tgtatcatac agaacccgtt ttttgtcgca
     9721 aatcggcttc agcgaccctc ctgccttatt ccgatttgaa tctgacttgc catacaaaca
     9781 ggcttcagac ggcattattt gcccgccaaa cgtatcccga tcttctccgc atattccctg
     9841 cgttcggcgc ggctggtttc cgggcggtgc gtattgagcg acgaccattt ccaatgactg
     9901 cgggctttgt tgagttcggg cgggagtttg gcggcatccc acggggcttt gcggctgtgc
     9961 agctcgatat ccgactgtgc cgcgtgtccg cgcgtttgca ggacgtggag cagatcgagg
    10021 gcgcgggcgg cgagcagggt cagggtttca gggtcggtgt gcagggtttg acggccggcg
    10081 agtttgtcgg aaatggtgcg ggtattgggc aggatgccgc ccaaaaagcc gccgattgcc
    10141 gtacccaatc cgagcgagcc gccgagtgtg gcgatgtcca gccccaagcc gatgagcgcg
    10201 ccggttgccg cgcccgtgcc ggtgcggatg ccgtattgtt tgagcaattc gctgtcgaac
    10261 gggtcttggc ggaaggcttg cggcatccag tcgccgccgt cgatttcgct gtggtagaaa
    10321 cggtaaaggg caaacagccg ctgctgcatc tgccgttcga gttggcgtat ttccgcctgc
    10381 atggtttgca gcacggtggc ggtatcctcg ttttcgtcca cttcctgcct gaaggcggcg
    10441 gcatcgagca ggaaatcggc gatttcgcgg cgcgcttcgc cgtccagccg ctgccattcg
    10501 cgccggcgca tggctgtcag gcggtcaagt gtgctgcgtt cgggcaacat ggtggcgagg
    10561 ttttcccaca ggcgcaattc gccttcaaaa tcaaaggcga cggtgtcgaa ccctgcgaaa
    10621 acgtgcaggt ttctcctcgc cagcatggtt gtccacgatt cgggaagctg tccgccggta
    10681 aagttgaaca cgggcataac cggtttggca caccatgaaa ggatggtcaa ttcatcccga
    10741 tatttgtcga ggacgggttc gcgcgcgtcg atgacgtaca ttgccatatc gctttgcaaa
    10801 acctgccgca gcactttggc ttcttggttg aaatcgtgat gcgcgccgtg gctgccgaga
    10861 aactgttgca gccgctcgat gccgtctgaa cgattgtccg tatggttttc cagccattcc
    10921 aaaacaccgc ccgcgtcttc gagtccgggc gtgtcataca ggaaaaccaa catgtccgcg
    10981 ccgtcgctga tggcggcttc ttcgacatgg cgtgtggtgg acggtgcgtt tctgacttcg
    11041 ccgaagccgc tgtcgcgcaa cagggtgcgc aggagcgagg ttttgccggt gttggtgtgt
    11101 cccacgacgg cgagggaaag gggttgtttg ttcatgatgt ttttgaagaa tggattttca
    11161 gacggtcttt tttcagaatg gcggcttaac agaacatttc aagtgagttt attggtcttt
    11221 caaacggcct tcctgcgcca ccctgtcagg ctcaagccac gccgcgccgc attcggtcag
    11281 cgcgttacgc caatgttcca gcttttccga aaggtcgtct gaaagcccct gttccgccaa
    11341 aagctgcacc accgcgccgc cctgcgccgc ttccgaaagc cgcacaatct gccgcagcac
    11401 gccccggtcc ggcacagttt gggcgcgtac gccgataagc agttgcgccg gtttctgctt
    11461 cagctctgtc tccagcgcgg caacctgttc ccgattggcg gcaacgccct tatccagcca
    11521 ttcctgcgcc agcctgccct cgaaccattg gccgtcctgc cactcggtct ccagcatgag
    11581 cgcccatttc ggcgcatcgt tcaagacgat tttcggcgaa acggcggaca cggtttcccg
    11641 acgcgtatcc gcatcggtga ttttgttctg ccagcggcgg atgaccgcct gataataggt
    11701 tttttccaaa tccaatccgt tttcgcttgt tttcaaaagg attttacaca ctacccaagc
    11761 caagaggcgc ggcaggatgc cgtagcagac gatactgccg accagcagcc ccgaccaagc
    11821 ccgcgcatcg gcaatattgc cgttcagacg accttcgatg accgcccgcg catcggggac
    11881 agggaaaccg agtttcgacg gcagccatgc caacatttcc accgcgcgta ccgaagcggc
    11941 attgctcaac agcgtgcttt cccagttgaa cgtatattgc cgcaccaaaa gcagcagcaa
    12001 taccgacacc agcattccga gcagcgtgca gagccacaag ctgtgcgccg ttgcgcctat
    12061 tttccatcgt accgaaggtt gccgccactg gtccgcatac agccgcaaca ccgcctgatt
    12121 tacagggcct ttgccccgaa accacgtcgc cggactgctg aaaaaccgcc ccactttcac
    12181 gcgcaggaac aacgttgcca accatactgc cagcatcagc gtattcatgc ccaacactcc
    12241 cgccaaaact aaaaagaaat tcagcccctg attgtccatc agaagataag tgcctgaaaa
    12301 tccggcggta aacatcatcg atgccaccac cacccataac cagaacgacc ccgcacgcac
    12361 acgttccaac gtgtcccgca gcatacggtc cctgtcgatc atctccgccc gacggatgat
    12421 tttttcctcc gtactgccgt ccacgcggcg caaagcctcc gtcgcctgca cgggatcgcc
    12481 gctgaaaata aaaccgcctt catccaaaat acggaccagc tcaaccagtt ttcgggatgg
    12541 attcaacata aaatgccgtc tgaaaataaa aaacaaattt taacacacgc attttcaaga
    12601 atattcacag tgtaggcaaa gagtaaatcc cacacagagg caaaagtatc ggcgtaaact
    12661 gactgcctct actttcccga aagattgtgc gatgtatgca ggcgaacgct tcaatactta
    12721 cagccatttg agcggtttga ttctggcggc ggcaggtttg atgctgatgc tgctgaaaac
    12781 cataggacac ggggacggct accgtatctt cagcgtatcg gtttacggca tcagccttct
    12841 tctgctctat ttgagttcct cgctgtacca cggaattgca gccggaaaac tgaaaagcat
    12901 tttgaaaaaa accgaccact gcatgattta tgtgctgatt gccggaagct acacaccgtt
    12961 tgcactggtt tctttgagaa acgggccggg ctggacggta ttttcactgt cctggctgct
    13021 ggcggctgca ggaatcgcac aagaactcac catcggacgg aaaagcgaaa aacgtctgct
    13081 gtctattgcg atttatatcg taatgggctg gatggtcttg gcggtaatga aatccctgac
    13141 agcctcactc ccgccggcag gactggcttg gctggcggca ggcggtatgc tgtacagcgt
    13201 cggcatttac tggtttgtaa acgatgaaaa aatccgacac gggcacggaa tctggcatct
    13261 gttcgtattg ggcggcagca taacccaatt tgtcagcgtg tacggttatg taatctgaat
    13321 gccgtctgaa acacaaaaac ctcccaaacc tgaaggcagg gaggttttct ctttgccggg
    13381 cattattgtt tatcgtggaa ctcgtggaat tcatactgat aggataaatc ctgtcccgcc
    13441 tttttctgca tcaggtagtc atcataaatg gcgcggattt ccttacgcag caagaataag
    13501 gcaatcaggt tggggataac cataaagccg ttgaacatat cggacagatt ccaaaccaaa
    13561 tcgactttgc cgagcgtacc caaaacgata gcaagcagaa ccaatgcgcg atagatgccc
    13621 aagtgtcttc ccctgaaaag aaaacggatg ttggactcgc cgaaataata ccagccgata
    13681 atggtcgtaa aggcgaagaa ggtcagacac acggcaagca attgcgaacc gaagcccgga
    13741 aatgccttgt taaaggcaaa ttgagtaacc gccgcgccct gttcgcccga aaggttggca
    13801 tcggtcagca ggataatcaa tgccgtagcc gtacatacca aaatcgtatc gataaataca
    13861 ccgacaaatg ccgtcatacc ttgctgaaca ggatgcttca catccgcagt cgcgtgggcg
    13921 tgcggagtcg aacccatacc tgcttcgttg gaaaacagac cgcgcgccac gccgaaacgt
    13981 atcgcttcac gcataccgat acccgcagca ccgcccaaaa cggcctcggg attgaaggcg
    14041 gcggtaaaga tgtggttgaa catcggcaca atatggtcgg aaaattcaaa caggataacg
    14101 acggcgcaca aaatataaac aaccgccata aacggcacga caaaccgggc aatattggca
    14161 atacggttca cgccgccaat cacaaccatg cccgcaagga cggcaaacac aataccgact
    14221 gccaaagaag gcacatcaaa tgcaatggta acggcagaag cgatggaatt tgcctgagtc
    14281 gcattgccga taaagcccaa cgcgacaatc agcgcgatgg agaaaaagcc cgacaggaaa
    14341 cgcgccgcgc ccctgccgat tttcggagtc agaccgtgcg taatataaaa cgccggcccg
    14401 ccgatgtatt tgccgtggct gacgacgcgg tatttctgcg ccagcagtgc ttccgcaaaa
    14461 atcgtggaca tccccaaaac ggcagaaagc cacatccaaa aaatcgcacc cggcccgcct
    14521 gcggtaatgg cggtcgccac accggcgacg ttgcccgtac cgatttgcgc ggatatagca
    14581 accgccaacg cctgaaactg cgataaggac ttatcgtctt tatcaccttt ggcaaacaag
    14641 ccgccgaata cggatttgaa tcccgcgccc agcttggtaa tctgcggcgc accaagatac
    14701 agcgtaaaaa acaggccgat acccaaaagc gcataaatca gcaggtagcc ccaaagaaac
    14761 cgattgaccg tacccaccag aacagacaat atattttcca taaaataaac cttatcttac
    14821 gattaaaatg actgttttcc aaaagacatt ccaataagga aacacggcga gcagaccgta
    14881 tttgccgcaa cagatgcctt aaattgtcaa caatccgaga gaagctgtgc cagcataccg
    14941 taaaatatcg ttaattaaaa tatttcttta tttttaagcg gaaagcggag gaaatcgttt
    15001 tcagacggca tcgacaacgg cacggcataa aacaggatat tttgggtact tgcaacttat
    15061 gttaaaatgc cgaccgtaaa aaatctgaca aaaacagatt aattatttga aataagaaag
    15121 gaaatttatg gcaggccata gcaagtgggc gaatatccag cataaaaaag cccgtcagga
    15181 tgccaaacgc ggcaaaatct tcacccgttt aatcaaagaa atcaccgttg cggcgcgtat
    15241 gggcggcggc gatcccggcg caaatccgcg cctgcgtctg gctttggaaa aagcagccga
    15301 aaacaatatg cccaaagaca atgtgcaacg cgccatcgac aaaggtacgg gtaacttgga
    15361 aggcgtggaa tacatcgagt tgcgctacga aggctacggc atcggcggcg cagctttgat
    15421 ggtggactgc ctgaccgaca acaaaacccg caccgttgcg gacgtacgcc acgcatttac
    15481 caaaaacggc ggcaacttgg gtaccgacgg ctgcgtggcg ttcaacttcg tgcatcaggg
    15541 ctatttggta ttcgaacccg gcgttgacga agacgagctg atggaagcgg ctttggaagc
    15601 cggtgcggaa gacgtggtta ccaacgacga cggttccatc gaagtcatta ccgcgccaaa
    15661 tgattgggcg ggcgtaaaat ccgctttgga ggcggcaggt tacaaatccg ttgacggcga
    15721 cgttacgatg cgcacccaaa acgaaaccga actctccggc gacgatgccg tcaaaatgca
    15781 aaaactgatt gacgcgctgg aagacttgga cgacgtgcaa gacgtttaca cttccgccgt
    15841 attgaatctg gactgatacg cagcacagca gacatacaac aaaatgccgt ctgaaccttt
    15901 cagacggcat tgatttattt agcccttgcc cacgcccacc aaaccgtcag gacaaccgcc
    15961 aaaaaatacg ccgcgctcca aacaggcaaa gcaaccccta acagataatc cggttcggca
    16021 caatttccga acccgcgcac aacaggctcg aaccaatcaa acaaaggcca gcccttcaat
    16081 cgaaacgtcc acggcgcgcc gcacgaagga gccgtacccg gcggcagcga ctgcaaccac
    16141 aactgatatg ccgcaacaga aatacccgta acggcgggaa tactgataaa cactgcgcct
    16201 gacagtccgc ccaccctgcc cttgggctta cacgccagca caactgccgc acacaatgcg
    16261 gttgccaaaa cacataaccg ctgactgatg cacaaaacgc aaggctccat acccaaaaca
    16321 tactgtgccg ccaaagaacc ggcaaatgca cagaccgaaa cggcaaacag cagccaaacg
    16381 gcttttctaa ataacggggt cattttctca acacaccaat caaaataccg atatgccgat
    16441 tttgctggat atatcccgag atggcaaggg acgaatcgac accggcacag gtgcggacga
    16501 tttcggtttt cccgatatgc acaaaacctg acaaaggaca aaacggcgat ttgtccgcac
    16561 aatgcccgaa aaaatacatc cggaggattt gaatttgaat ttcggcaaat ttagcgaatc
    16621 aaaagcaaca gccgataaaa aaatcaaaag cttatttcaa tgtttcaaca cacaggacga
    16681 cacataaagc accgccctat gtgccgtcct gatttggaag gggttacacc cctcccaaat
    16741 atagtggatt aacaaaaacc ggtacggcgt tgcctcgcct tgccgtacta tttgtactgt
    16801 ctgcggcttc gccgccttgt cctgattttt gttaatccac tataaagtct gattctaccg
    16861 ccctaaaggg cagggtttca accgaaaagg aaacacgatg aaagcatatg attttttgaa
    16921 taatcggatt gacgggtttt tgagggaatt tgttaccgga tagccatcgg gcaagttttt
    16981 tgcaaaattt gaatcggtcg ggtaaatttt caaaaaataa ttgacagcag ataagaaacg
    17041 gcggataatt accgccgttg agttgcttga tgcagcttga tttttctcct ctacttctcc
    17101 tttgtagact tggcacacat tcaaccggat gtgtgcattt tttatctccg ctttttttga
    17161 aatttaatta cttttattgt ttgaaattct attctttaag aaatttaaca agaaaaacgc
    17221 ttgctttttg accggaaaag ctgcataatt cgttccgtta gctggttcga gtagtcagtt
    17281 aatagtttct cctctatttc tcctttgtag acttggcaca cattctattg aatgtgtgca
    17341 ttttttatct gaagcaacaa gcctctgtgc gtgatgttgt tatgtttcat ttagatgtca
    17401 aaccgcatac ccggtctgaa atattcaatc caaaaccgga ttttctttga cttcctccat
    17461 cacaacataa ctcctactct ccgaagcggc aggcagttgc aataggatat tgccgagcat
    17521 atcccgatag gcagacatat cgggcaaacg tactttaatc aggtagtcgt attcgcccga
    17581 caccagatgg cactccataa tttgcggaat tttcagcact tcttttttga aatcttcgaa
    17641 aatattgccc gatttggatt gcagcttcag ctcgacaaaa accaatggcg gttttcccaa
    17701 cagatgggga ttgagatggg cgtgatagcc ggaaatataa tgttcccgct ccaaacggca
    17761 taccctctct gtaacgggcg tgctggacaa ccctaccttc tcggcaagct ccgtcatcgg
    17821 gatacgggca ttttgctgaa gaattttgag gatgcggaaa tcgattttat ctagttcttt
    17881 catttggatt accttgtatt tattattgat tttaacaaat agagtataaa tttgggaaag
    17941 caattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctc tggcttgaag
    18001 acacaaggtt ctttaatatt ttaaaagcct tgccgttgga ttataattcc ccaattgatt
    18061 tcttaatttt gctaataaac acttgtttgg taaggaatga atttatgcgg cctttgaacg
    18121 tgcagatcag gttgggcaac cttaggcaca attatcggat tttgaaggaa atgcacggag
    18181 gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc agatgtgctt
    18241 tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgacgag ggaatcaggc
    18301 tgcgggagag cggcattacc catccgattg tccttttaga aggcgtattt gaagcatcag
    18361 agtacgaagc ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa tggcagcttg
    18421 aggctttgct ttcccgccat tggaaaaaac ctgtcaaggt ctggttgaaa atggattcgg
    18481 gaatgcaccg taccggattt ttccctcatg attacgcttc ggcatatgcg gcattgaaac
    18541 agtcggaata tgtggacagt attgtcaagt tctcgcattt ctcctgtgcg gacgaacctg
    18601 aaagcggtat gacggaaata cagatggaag cattcgattt gggtacgaaa gggctggaag
    18661 gcgaagaaag ccttgccaat tcggcagcta ttttgaatat ccccgaagca cgcagggatt
    18721 gggggcgcgc gggcttggcg ttatacggca tttccccgtt cggaggcagc gatgacaggc
    18781 tgaaacccgt gatgaggctt tcaacccgta ttttcggcga acgcgtttta cagccgcact
    18841 cccctatcgg ttatggcgca acattttata ccagtaaatc tacgcgcgtc ggtctgattg
    18901 cctgcggtta tgcggacggt tatccgcgcc gcgctccaag caattccccc gtcgccgtcg
    18961 acggtaaatt gacccgggtc atcggcagga tctctatgga tatgatgact atcgagctgg
    19021 acgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg gtcaatatca
    19081 ataccgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat atcaaacgtg
    19141 caaaattcac ttatatcgag taatcgagtc caaacgaaaa tcccgtctga agcttttcag
    19201 acggcataaa tgcgttttga caaaatcagt aaaagacatt gccaaataga aaatactgat
    19261 aatgcccaca atctgaaacc ccatccttcc cgcaaaaacg gaaaatcaaa atcagaaacc
    19321 taaaatttcg tcattcccgc gcaggcggga atccggtttt ttgagtttcc gtcatttctg
    19381 ataaattctc gtcgtttttc atttctagat tcccgcatgc gcgggaatga caaaattaaa
    19441 gtttcagaat ttatttgaac aacaaactag attcccgctt tcgtggggat gacggcggat
    19501 aggttttgtt gtttcggata aattcctgtt gttttttggt ttccagattc ccacctttaa
    19561 aagaatgacg taagaaagca gaaataagaa cacggggatt ttttaaattt gcaatattct
    19621 tttctaaaga cattttaccc aatgcccata aacaaaaatg ccgcctgaag cctttcagac
    19681 ggcattttcc catcaaaacc gcaatcagtt tttcatcgat tgaaccggtg cgggaatcct
    19741 gccgccccgg ttgacgaaca cttcgcacga accttctttt gccggcatta caggcgcgta
    19801 gcccaacaga ccgccgaact cgacgctgtc gccgacggtt ttgcccgtta ccggaataat
    19861 gcgcacggcg gtggttttgc tgttgatcat gccgatggcg gcttcgtcgg cgatgatgcc
    19921 ggaaatggtg tgcgcgggcg tgtcgccggg aacggcaatc atgtccaaac caacggagca
    19981 gacggcggtc atggcttcga gtttgtccag cgtcaacacg cctgcctcgg cggcggcaat
    20041 cataccttcg tcttcggaaa cggggataaa cgcgccgctc aaaccgccga ccgcgctgga
    20101 agccatcatg ccgccctttt tcacggcatc gttcagcaat gccaaagctg ctgttgtgcc
    20161 gtgcgtaccg cagacgctca agcccatttc ttcaagaatg cgcgccaccg agtcgccgac
    20221 ggcgggggtc ggtgccagcg acaaatcgag aatgccgaac gggatattca gcatttttga
    20281 ggcttcgcgg ccgatgagtt cgcccacgcg ggtgattttg aaagcggttt tcttcacgac
    20341 ttcggcgacc tcggtcaggc tgaccgcgtc cgaattttcc agcgcggctt tgaccacgcc
    20401 tggaccggat acgccgacat taatcacagc atccgcttcg cccgagccgt ggaacgcacc
    20461 cgccataaac ggattgtctt ccaccgcgtt gcagaacacg acgattttgg cgcagccgaa
    20521 accttcgggc gtgatttcag ccgtgcgttt gatggtttcg cctgccagct tgaccgcatc
    20581 catattgata ccggcacgcg tgctgccgat attgatggag ctgcacacga tatcggtagt
    20641 tttcatcgct tcgggaacgg aacggatcaa cacctcatcc gaaggcgaca tacctttttg
    20701 caccagcgcg gaaaagccgc cgataaagga cacgccgatg gctttggctg ccttgtccaa
    20761 agtctgcgcc acgctgacat aactgtcggc tttggtcgcc gccgcgattt gggcaatcgg
    20821 cgtaacggaa atgcgctgat tcacaatcgg cacgccgtat ttggcggaaa ggtgtttcgc
    20881 cgttgccacc aagtctttgc cgaccgtggt gattttgttg taaatgtttt ggtttaacac
    20941 gtcgatgtcg gtgctgatgc agtcgtgcaa atcaatgccg atggtaatgg tgcggacatc
    21001 aaaattctgg tcggcaacca ttttgacggt ttctaaaatt tcgccggatt ggatactcat
    21061 cacattcctc cgactcaaat gcggtgcatc gcttggaaga tttcttcgtt ttgcatacgg
    21121 atgtcaagcg cgagtttttt gctctcttcc gcaaacaaat ccaaaatctc ctgccgcgat
    21181 ttagggcatt tcgaggtatc caccaagata atcatagtaa aaaaatcgtc catcagctgt
    21241 tggctgatat tgaggatgtt gattcggttt tccgccaaaa ttttggaaac atcgtacacg
    21301 atgccgacgc ggtctttacc gatgacggtg atgactgaat tgttcacagg cttactcctt
    21361 gcagatatcc gttaaaatcc gaaattatac caccgtcgga ttttgaagaa tattgtcaac
    21421 aatatataca tacaaaatgc cgtctgaaac tatttcagac agcatcaaga ttcagggttc
    21481 gattaaataa ccatccttat cccattgggt tttcctgacc aacttgtcat cctgataaac
    21541 agcctcgctc tttttagaac catcttcata ccactccaaa accaccccgt tgcgtttatg
    21601 gtggcggata gacagttccg aaagtaatcg gccgctttca tcccaagtca gaattttggc
    21661 aggctcatcg ttgaccataa ccatttccgt cttgatactt ccatcggcat accattgctt
    21721 ccatacgccg ttcgccttat tttgcttaaa ctggatttcg ctttccttgc cgccgttacg
    21781 gtaataacgg tatcccgtac cctcactcaa gccattttta taaggcataa ccgcagattt
    21841 tttaccgttc ggataccagt tgacccattc cccgtccggc ttacccttgc tgaagccccc
    21901 cgccattttt ttctgaccat taaaatgcca caaaatcaac ataccgtttt gcagggtagg
    21961 cacaaaagat ttgatttgcg ttgaagcaac gatataaggt tcggaatatt tcttcatcga
    22021 cggataataa aaatcctgcg cgtgtgcgat acccgccgcc acactatatt gcctgatata
    22081 agcggcagaa gacatcgtcg ccgtcagttt cccgttctga ttaaaataaa cagaataggt
    22141 ctgcgccggc aaagcggccg aaaaacccaa cagtacgatt gaaaatacaa tccgagataa
    22201 tttcttcatt atgatagcga tataaaaaac aattaactta taaacaatat ggtaaggatt
    22261 tctcaaccaa gcgtcaaacc cgaaacaatg tatcgtaaaa atgccgtctg aaaacaaatc
    22321 gtcttcagac ggcatttccc tccaactcac tcttcgccca ataactgctc gcgcgtcaag
    22381 aggaaaacaa aaccgtcgcc cccgctggtt tccaaccaag taaaaggcaa ctccggatac
    22441 gctgcttcca atacatccct gttatgcccg atttccacca gcaatacacc tttaggattc
    22501 agaaactttg ccgcattcag aagaatctgc ctggtggcat ccaacccgtc cgccccgctg
    22561 cccaacgcca attccggttc gtgcaaatat tcctcaggca aagccccaac cgactctgca
    22621 tccacataag gcggattgga aacaatcaaa tcataagtgc cttccagtcc ctcaaacaaa
    22681 tcggtatgaa taagctgaat acgctcttcc agaccataat cttcaatatt gatccctgca
    22741 acttccaaag catccaaact cacatcaacc gcatcaattt gggcatcagg ataatggtgc
    22801 gccatctgaa tggcaaggca accgcttccg gtgcaaagat ccaaagcatt atgcaccagc
    22861 tcatcgtatt ctatccaagg tcgaagtccg tcacccagca attcataaat aaaagagcgc
    22921 ggtacaatca cgcgttcatc tacataaaaa tcaaactccc cctgccaggc ctggtgtgtc
    22981 aaataagcgg caggaatgtg ttcgacagca cgacgctcaa taaccgccag cacttcctct
    23041 ttttcagctt ccaagagttt tgcatcaaga tatggggcaa gcatatccaa aggcaaattc
    23101 aaagtatgca gaatcaaata aaccgcttca tcatgcgcat tatctgttcc atgaccaaaa
    23161 aagagccctg cctcattaaa acggctgact gcaaaacgta aaacatcacg gatagtcgtc
    23221 aattcttgtg cggcctgatt aaacataata tgaaccattc tgcgtataga tgcttttaat
    23281 tataacagaa acaacaagca aaccttttca tatcgccaaa taaccactca atctacccat
    23341 acaactacat aaatgccgcg cgaagaccat tgcccgaacg gaaacggcga tggcggacgg
    23401 tatgggcaac ctgataggct gggaagaaac ggggcttgtt gagggcaagc agtggataac
    23461 cgcaaaagac gacaaggtgt ccgatgtctg caatgccaac ggcgggatgg gcgtaatcgg
    23521 tttgcacgag cctttctccc acggtgcgtt gacgataccc gagcatccaa atggcaggtg
    23581 cgcggttgtt cccgttgtcg ggcggacagg caagctggcg aaaggcagcc gcgcgatgga
    23641 aacgggcgat ggacggcagg tgaggttgtt tccgtatcgg gcggcgaatt gggggaattt
    23701 gctgaaacaa aggagcttcg taaagcggct atgcagtatg cgcgtgataa ctttgtcggc
    23761 aaaagctatg tcaatgaaag cagcgggcat gaactgaagg taacttggca aggtgtgaaa
    23821 cacgctgcgt caaaatcgaa cagcatcgaa ctttccctta tggtcgcgct tgataaagtt
    23881 ttgatgaatg caaaatatga aggaagtttt cctaacaaat tagggaggga tcatattatt
    23941 gcggtacaca aatactcaac cggtgtcaga tttggcaata aagtcttaaa aatcaggata
    24001 atagcccgtg agaaatttga tgggataaaa cattacgatc attttatttt gaaagacaaa
    24061 tgaaaaaacc ctgtccagta tatctgggat agcttatgga agcatttaac ccagcctcgg
    24121 ataaggcttt aaatccatca tgccatttat acggtaaaaa caagggtctg tactagatta
    24181 gcagatatgt taccctcgaa atatgaagat aacacactgc aaattaaaga aagaagtaca
    24241 gaaagaaccg c
//
LOCUS       DAXFZR010000024        24190 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000024 DAXFZR010000000
VERSION     DAXFZR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24190
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.024"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(69..2348)
                     /gene="nrdA"
                     /locus_tag="ACFJAS_000828"
     CDS             complement(69..2348)
                     /gene="nrdA"
                     /locus_tag="ACFJAS_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC3982120.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEVRLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            2879..3190
                     /locus_tag="ACFJAS_000829"
     CDS             2879..3190
                     /locus_tag="ACFJAS_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC3982121.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
     gene            complement(3240..3350)
                     /locus_tag="ACFJAS_000830"
                     /pseudo
     CDS             complement(3240..3350)
                     /locus_tag="ACFJAS_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3453..4220)
                     /locus_tag="ACFJAS_000831"
     CDS             complement(3453..4220)
                     /locus_tag="ACFJAS_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC3982122.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTFSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(4308..5135)
                     /gene="mutM"
                     /locus_tag="ACFJAS_000832"
     CDS             complement(4308..5135)
                     /gene="mutM"
                     /locus_tag="ACFJAS_000832"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC3982123.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(5188..5853)
                     /locus_tag="ACFJAS_000833"
     CDS             complement(5188..5853)
                     /locus_tag="ACFJAS_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC3982124.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            6032..8008
                     /locus_tag="ACFJAS_000834"
     CDS             6032..8008
                     /locus_tag="ACFJAS_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC3982125.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVIPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            8076..8768
                     /locus_tag="ACFJAS_000835"
     CDS             8076..8768
                     /locus_tag="ACFJAS_000835"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC3982126.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRAQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            9243..10613
                     /locus_tag="ACFJAS_000836"
     CDS             9243..10613
                     /locus_tag="ACFJAS_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3982127.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITTIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            10846..11502
                     /gene="cmk"
                     /locus_tag="ACFJAS_000837"
     CDS             10846..11502
                     /gene="cmk"
                     /locus_tag="ACFJAS_000837"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC3982128.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFAFERILSDIETRDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            11655..13340
                     /gene="rpsA"
                     /locus_tag="ACFJAS_000838"
     CDS             11655..13340
                     /gene="rpsA"
                     /locus_tag="ACFJAS_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC3982129.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            13351..13665
                     /locus_tag="ACFJAS_000839"
     CDS             13351..13665
                     /locus_tag="ACFJAS_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC3982130.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(13861..14268)
                     /locus_tag="ACFJAS_000840"
     CDS             complement(13861..14268)
                     /locus_tag="ACFJAS_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC3982131.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            14418..15528
                     /locus_tag="ACFJAS_000841"
                     /pseudo
     CDS             14418..15528
                     /locus_tag="ACFJAS_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            15537..16364
                     /gene="fghA"
                     /locus_tag="ACFJAS_000842"
     CDS             15537..16364
                     /gene="fghA"
                     /locus_tag="ACFJAS_000842"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC3982132.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNRENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            16894..18045
                     /gene="zapE"
                     /locus_tag="ACFJAS_000843"
     CDS             16894..18045
                     /gene="zapE"
                     /locus_tag="ACFJAS_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC3982133.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            18104..18529
                     /gene="ndk"
                     /locus_tag="ACFJAS_000844"
     CDS             18104..18529
                     /gene="ndk"
                     /locus_tag="ACFJAS_000844"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC3982134.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            18673..19767
                     /gene="rlmN"
                     /locus_tag="ACFJAS_000845"
     CDS             18673..19767
                     /gene="rlmN"
                     /locus_tag="ACFJAS_000845"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC3982135.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            19770..20531
                     /gene="pilW"
                     /locus_tag="ACFJAS_000846"
     CDS             19770..20531
                     /gene="pilW"
                     /locus_tag="ACFJAS_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC3982136.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            20547..21812
                     /gene="ispG"
                     /locus_tag="ACFJAS_000847"
                     /gene_synonym="gcpE"
     CDS             20547..21812
                     /gene="ispG"
                     /locus_tag="ACFJAS_000847"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC3982137.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(21866..22480)
                     /gene="clpP"
                     /locus_tag="ACFJAS_000848"
     CDS             complement(21866..22480)
                     /gene="clpP"
                     /locus_tag="ACFJAS_000848"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC3982138.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(22576..23886)
                     /gene="tig"
                     /locus_tag="ACFJAS_000849"
     CDS             complement(22576..23886)
                     /gene="tig"
                     /locus_tag="ACFJAS_000849"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC3982139.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
BASE COUNT         6264 a         6471 c         5645 g         5810 t
ORIGIN      
        1 atttaagaaa atccttgaag cctcctacta tctagaagcc ttggatatat ttcaaaaatt
       61 aacctgtttt aaatcttaca agccccacca gcgcagccgt catcttgaat atcggtctgc
      121 gtgtcgtccg cgccgtcgcg ggtgttgtgg tagtacaggg ttttgacgcc gtatttgtag
      181 gcagtcagca ggtctttgag catttgtttc atagaaactt tgttaccttc gaatttgccc
      241 gggtcgtaag cagtgttggc ggaaatcgcc tgatcgacga atttttgcat tacgccgaca
      301 agtttcaggt agccttcgtt gcccggaagc tgccacaggg tttcgtaggc atttttcaag
      361 gtttcaaact ccggcacgac ttgtttcaaa atgccgtctt tcgatgcttt gaccgttacc
      421 aatccgcgcg gcggctcgat gccgttggtt gcgttggcga tttgcgagct ggtttcagac
      481 ggcatgagcg cggtcagagt agagttgcgc aggccgtatt tgacgatgtc ggcacgcagg
      541 ctttcccagt cgtaatgcaa aggctcgccg cagacggcat ccaaatcttt tttgtaggtg
      601 tcgatgggca gtttgccttg cgaataaacg gtttggttaa agagcgtgca cgctccgtat
      661 tctttggcaa ggtttgccga tgctttgagc aggtaatact gtatggcttc aaaggtacgg
      721 tgggtcagac cgagcgcgga accgtcgctg tagcggacac cgtttttcgc cagatagtag
      781 gcgtagttga tgacaccgat gccgagcgaa cgccggtcca tagtagaggt acgcgcggct
      841 tctaccggat atccctgata atctaaaagt gcatcgagtg cgcgcacggt caaatcggca
      901 agcccttcca attcgtccaa gctgtttaat gcacccaagt tgaaggcgga cagtgtacac
      961 agggctattt cgccgtcagg gtcgttgatg ttgtccagcg gtttggtcgg cagggcgatt
     1021 tccatacaca agttggactg atgaacaggc gcaacgcgcg gatcgaacgg gctgtgcgta
     1081 ttgcagtgat cgacgttttg aatgtagatg cgcccggtcc cggcacgctc ctgcatcagc
     1141 gtggaaaaca ggtcggcagc cggaatgata cgcttgcgta tgttcgggtc ttgctcgtat
     1201 ttcgtataga gccgctcaaa ttcgtcttgg tcggcaaaaa acgcttcgta caaccccgaa
     1261 acctcgttgg gcgaaaacag cgtaatgttg ccgcccttaa tcaggcgggt gtacagcagg
     1321 cggttgattt gcacgccata atcaagctgg cggatacggt tgtcttccac gccccggttg
     1381 tttttcaaca ccagcaggct ttcggcttca atatgccaca aggggtagaa caaggttgcc
     1441 gcgccgccgc gcacgccacc ttgcgaacaa gatttgaccg ccgcctgaaa catcttaaag
     1501 aagggaatgc agccggtatg ccgggcttca ccgccccgga tttcgctgtc cagcccgcgg
     1561 atacgtccgg cattgatgcc gatgcccgcg cgttgggaaa cgtatttcac aatcgcgctg
     1621 gtagtggcat tgatggaatc caaactatcg tcgcattcaa tcagcacaca gcttgagaac
     1681 tggcgcgtag gcgtacgcac gccgctcata atcggcgtgg gcaacgatac tttgaatgtg
     1741 gaaacggcat cgtaaaaccg tttgacgtaa cccaagcgca cctctttcgg gtatttgctg
     1801 aaaaggcaca tcgccaccaa aacatataaa aactgcggcg tttcgtaaat ctggcgggta
     1861 acgcggttct gtaccagata tttgccttcg agctgtttga cggcggcata ggaaaaggac
     1921 atatcgcgtt cgtggtcgat ataggcgttc agttcgtcaa attcttcgcg gctgtaatcc
     1981 tcaaggatat gcctgtcgta ttttccggca tcggtgagct ttttaacatg gttgtaaagg
     2041 tgcggcggct cgtattcgcc gtaggctatt ttacgaagat ggaaaatcgc caaacgtgcg
     2101 gcaaggtatt ggtagtccgg ggtatcttcc gaaattaaat ctgcagcggc tttgatgatg
     2161 gtttcgtgga tgtcgtcggt gcggatgccg ttgtagaact ggatgtgcga tttcaactcg
     2221 acctgcgaca cggaaacatt ttccaatcct tccgccgccc aagtaacgac acggtgaatc
     2281 ttatccaaat caatggcttc caatcttccg tctcgtttgg ttacttttaa atcagtcggt
     2341 gtattcatcg cttcctcttc cactcttgat attcaagaca cggtcttttc aaataaatta
     2401 aggcagacaa tatagtggat ttttagcatt ttctccagtc ttgacaacga ttgcattttt
     2461 cagtttcagg caatgcgcga taaaacccgc ctgtcgtatt tttcctataa catttgtttt
     2521 atctgataat tctttaccga taaaaaacgg gtaaattttt tgccttttga ccggctccgg
     2581 ctacaaggcg atgaaataag gattttccga cgaaaaagga aaacttccgg ttttccgccc
     2641 ctgcgaattg ttaaattttg caaagtatga tttttgccac gccgccgccg acaaattcca
     2701 ttttcttacc gattggaatt tattattgag attaatatgt tatttgaatt tgcatatcaa
     2761 acggcaagtt ttgtcggctg aatgagtctg aaactgccga cagtttgccc gttccatcct
     2821 tccatcctat actggaagga attacaaact gaaaggatcg tcatgtccgt caataaagat
     2881 gcttgccata ctgttgtctg caatactggg actggtatca acaactgccg ctgccggtac
     2941 gtcagaaccc gcccaccgac ataccaaaca tatcagcaag gcaaacaagc agatgctgca
     3001 ccccgaatgc aggaaatatt tggaacgccg tgccgcgtgg taccgatcgc aaggcaacgt
     3061 gcaggaattg cgcgaaaaca aaaaggcgcg caaagcattc cgcaccctgc cttatgcgga
     3121 acagaaaatc caatgccggg cggcttatga ggctttcgat gatttcgacg gcggcaggtt
     3181 ccgccgttaa tcccatataa aatatgctgt ctgaacacaa gttaagatgg cattttccat
     3241 agatatagtg gattaaattt agaccggtac ggcgttgcct cgccttgccg tgctatttgt
     3301 actgtctgcg gcttcgccgc cttgtcctga tttaaattta atccactata caacaaacta
     3361 tccttacggc atttcttttg aggtactccc gttaccgcag ccttaaggca tccgcaaaag
     3421 cgaatcatac cgccacattc cgacaaccgc tttcagacgg caacatccgc atcgcctgcg
     3481 acaacggcac ggatgctttc ttcgatttta tcttttaaag cataacggtc ttcgctttcc
     3541 gccgcgtccg ccacgcaaac gaaatcgact ttgatagtca gttttttcat agacacgatt
     3601 cgccacaggc aagtcggcaa accgacatcg gcatacgacg gacgggccgt cctttttccc
     3661 gtttcgtcat aataacgcag tgcgaccgcc aaaacctttg cccccgcatc gatggcggat
     3721 tggaacagcg cagccttgaa cggcaggagc cccagtccgg agaaagttcg cgcctcaggg
     3781 aaaaaactga cgttttgacc gcgttgcaag gtttcgcaga cggcgcggtt aatcggttcg
     3841 atgtcgcgcc gcgaattgcg gttgatgaac accgttcccg cgttctgccc catcttgccc
     3901 aataccggcc agcttttgat ttcctgcttg gcgataaagc tactcggata aaccgcgctc
     3961 atcgcgaaaa tatccagcca ggacacgtgg ttggcggcaa ccaagacacc gttcggatgt
     4021 tcgggtgcgg gtctgcccac ctccaatccg atatccaaag ccgccaaagc gcccttgccc
     4081 aacgctataa ctgcccgatt gcgcgacttg gggcagccgc cgtcaatacc gcgcaggttt
     4141 ttgccggttt tgaacagcca gaccgtcaag cggcacaagc ggcgcagacg tgtaaaaaat
     4201 gaagctttat ttgaagacat gggcaattct ttcccgataa tcggtgtttg ttgttgaaac
     4261 gttcggacgg cataccgata aaatgccgtc agaaaccgtt ttccgcccta tttctgacag
     4321 ttcgtgcaat aaaacgtgcc gcgctgcccc aaagtttctt tcacaaccaa accgccgcac
     4381 cggaggcacg gctgattgtg ccgcccgtac accgtatatt cctgttgaaa gtagccgctt
     4441 ttgccgtcgc tgtccacaaa atccctcagc gtactgccgc ccgtttcaat ggcgcgctgc
     4501 aacaccgctt tgacggtttc aaccaaaacc gcgcactctt tcttttttag gcggttggca
     4561 ggacggtggg gcgaaatgcc cgccctgaac agactctcgt tggcataaat attgcccacg
     4621 ccgaccacga cggtattgtc catcagagcg agtttgaccg cgcgtttctg cgccttcagc
     4681 cccgcataca gataatccgt gcaaaatgcc tccgacaaag gctccggccc cagtttttcc
     4741 aacagcggat gacgttcttc gattccctca taccaaagta tcgcgccgaa ctttctcgga
     4801 tcgcggtaac gcatgaccgt gccgtctgaa aacacaatat cgacgtgatc gtgtctgtcc
     4861 ggcctgccga tacgtccgtc cgacggcgta aagatccgca agctgcccga catccccaag
     4921 tgaatcagca aaatccccgt ttggaaacgg acaatcagat atttggcacg cctgccgcag
     4981 gacaaaacct gccggccgga caaaatctcc cccaaatcgg gattgatctg ccagcgcagc
     5041 ttcagttggc gcaatatcac ggcttccacc gtttttcctt caatatgcgg cgcgatgccg
     5101 cgcaacgtcg tttccacttc cggcaattcc ggcataaccc ctcccgacat ttcttctgac
     5161 agatgccgtc tgaaagacgg ccgtccctta atccgcaacc cttgccgcac ccgccgcaag
     5221 ggctttgccg ccccaatacc cgtcccacgc gggcaggggc gttacgcccg ccgccctttt
     5281 gaccaaaacc aaaccgtaca ctgcggcaca ttgcggccac caacggtcgc ccgccttttc
     5341 cataaaccgc caaaagcgta tttgcccgag cgacgaaacg ggaggcagat acaccatgaa
     5401 tttcccaaat tcaatatcga aaccggcatc ggcaagctgc cttttcaact cgggcagcgg
     5461 cagacaaaac cgtttttccg gcagacggac accgtcaaac caacggcaga atccccaaag
     5521 cgaatacgga ttgaaacccg tcagcatcaa acgtccggac ggtttcaata tccggtgcgc
     5581 ttccgacagg atttgcgaag gtacgccgca ttccagcgta tgcggcaaaa gcagcatatc
     5641 cgcaaaaaca tccgccaaag ccatattctc cgccgacatc gacatatctc gcggcacaca
     5701 gacaatgtct tcagacaggc tcagccactg accgcccacc tgaaccgcac acattcccga
     5761 aaaacggtat gacgccacat accgattaaa gaagtcctgt tccaattttg caacataccg
     5821 ccccatcgcc gtatcttcaa accatgcatc catattgcgt ccgtttcaaa cagattgcct
     5881 gccgatattt tattccaaac aggatttctg tcaaaaaaca catcggccgc ccatttccga
     5941 atccgcataa agttcctgta aaacttgacg ctttttcagt caaacagtac catcggacga
     6001 taaaatatgt ttattccgcc gagtataaat catgtccaaa ctcaaaacca tcgccctgac
     6061 cgcatcaggt ctgtccgttt gtccgggttt cctatatgcc caaaacacct catcacacca
     6121 agtcggttta gcgattatgc ggttaaactc ttcaatactc gacctgccac cgacaaaaca
     6181 atatttccaa tccggcagcc tgtgggacga gctgcgccaa ggcttccgga tgggcgaagt
     6241 caatcccgaa ctggtacgcc gccacgaaag caaattcatc gcaagccgca gctatttcga
     6301 cagggtcgtc aaccggagcc gaccctatat gtaccatatc gccaacgaag tcaaaaaacg
     6361 caatatgccc gccgaagccg ccctgcttcc cttcatcgaa agcgcgttcg tcaccaaagc
     6421 caaatcacac gtcggcgcat cgggcctgtg gcagttcatg cccgctaccg gcaggcatta
     6481 cggcttggaa aaaacaccgg tttacgacgg caggcacgac gtttacgcag ccaccgatgc
     6541 cgcactcaac tatctgcaat atctctatgg actgttcggc gactggccgc tcgcctttgc
     6601 cgcctacaac tggggtgaag gcaacgtcgg acgcgccgtc aaccgcgccc gcgaccaagg
     6661 gctcgaaccg acctacgaaa acctgcgtat gcccaacgaa acgcgcaact atgtccccaa
     6721 gctgctcgcc gtgcgcaaca ttattgccac cccccaatct ttcggcatga atatcagcga
     6781 catagacaac aaaccctatt ttcaggcagt cgaaccgggc cgtccgctcg acaacgaagc
     6841 catcgcccgg cttgccggca tcacgcaaag cgagctgctc gccctgaatc ctgcattcaa
     6901 cgtccccgcg ttcatcccca aaaacaaacg caaactgctg cttcctgtcg cgtccgtcca
     6961 aaccttccaa agcaactacc tcaacgccgc acccgacagc ctgttttcat gggaagtcta
     7021 tacgcctgcc gccaaaacca gcctgtccga catctcgacg gcaaccggca tgagcattgc
     7081 cgacatcaaa cgcctcaaca acctgaacgg caaccttgtc aacgcaggac gcagcatcct
     7141 tgtcgccaag aacggcaaga cccttcatac ggcatcggaa tccgtcgttt ccatcgacat
     7201 cgacaatacg cccgacacct accgttccaa tatgccggca ggcacggtga acgtcagcat
     7261 tgcccgaatc caacccgccg ccgcacagac agcggacatt accgtcgcac ctttgccgca
     7321 agaaaccgtc cgtacggaac ccgatcccct tgtccgcatt gccgaacccg cccttgcgac
     7381 agccgcagcg caacctcaaa ccgaaaaaca gactgccatg ccgtctgaaa cccaaaccgc
     7441 aacactcgcg caggtcatcc ccccaaacga catgcaggcg gcagacgaac tcatgcagct
     7501 tgttgcccga aacaacctgc gccggcaggc tgaagaaacc atctccgccg tcatcggcac
     7561 gcctgacaca gttgccgaac acaacatttc ctcatctccg caacataccg ttgccgccga
     7621 cggcaaacgc cgggcacgtt tggaaacgcg cgtagccaaa gctgccgacg gcgaagccga
     7681 aacctccccg ctccatgcca gcatccaccg cgttgtagaa ggcgacaccc tgttcaacat
     7741 tgccaaacgc tacaacgtca gcgtagccga cctgattgtc gccaacaaca tcaaaggcaa
     7801 caccatccaa aaaggacagg tactccgcct ggcgcaggca gcccctgccc aaacccgtat
     7861 tgaaaaagta tcctacaccg cgcgcaaagg cgacaccttc aaaagtatcg ccgcgcgctt
     7921 caatatccat atcgacgaca tccgccggct caatcccaac ctgaacacca tcaatccggg
     7981 acagagggtc aaactgattg gaagctgatt cggatacggc acatgacagg actttctcag
     8041 tcctgtcttt ttcatcccac atttcccata ccatcatgaa actcatcaaa tacctgcaat
     8101 atcaaggcat aggaagccgc aagcagtgcc aatggctgat taggggcggc tacgtctcca
     8161 tcaacggaac ctgcatggac gacaccgatg cagacatcga ttcctcatac gtcgaaacgt
     8221 tggatattga cggggaagca gtaaccgtcg ttcccgaacc ctatttctac atcctgctca
     8281 acaagcctga agattacgaa acttcgcaca aaccgaaaca ctaccgcagc gtattcagtc
     8341 tgttccccga caatatgcgg aacatcgata tgcaggcggt cggcaggctg gatgcggata
     8401 cgaccggcgt attgctgatt accaacgacg gcaaactgaa ccacaacctg acttcgccga
     8461 gcagaaaaat tcccaagcta tacgaagtaa cgctcaaaca ccctacagga gaaacgctct
     8521 gcgaaacttt gaaaaacggc gtgccgctcc acgacgaaaa cgaaaccgtt tgtgccgccg
     8581 atgccgtttt ggaaaatccg accaccctgc tgctgaccat taccgaagga aaataccacc
     8641 aagtcaaacg catggttgct gccgccggca accgcgcgca acaccttcac cgccggcgat
     8701 tcgcacattt ggaaacagaa aacctcaaac ccggggaatg gaaatttatc gaatgtccga
     8761 aattctgaaa caaaattcca taatttccct atattcaacc atatgcttat taaataagca
     8821 gttttacaaa aattaactca aaaaaacagt tagtttacaa tataatccgc ttgtctaagt
     8881 aacgcctgtt caagcaggat tatgagcaag acgttttccc cgtaatgtgt tttgccatct
     8941 atacttctcc ccttgtatag atggttttct atttcaaatt ccctctcttt catcatcatt
     9001 gaaatgctat cattaatatg attaacaaaa tttcacttcc gtacaccgat gtagaaaaat
     9061 aacagattaa tctgttcata aactccgatt cttcctcatc ggtttatact catcagaaat
     9121 attcactgaa atatatgcat tttacatgga aaatcactag gcagttgaaa aacattcagt
     9181 caatttctgt taagaatcac gtgtaaaatt tccgctttat ctcaatcgga cacggtttca
     9241 atatgtcttc cccttcaaat accaaccgtc aaacctggtc cagccgtttg acttatatcc
     9301 ttaccgttgc cggcgcgact gtcggtttcg gcgcgacgtg gcgtttcccg tatttggtcg
     9361 gtgaaaacgg cggcggcgcg tatgtgtttt tattctgtat cgcgatgctg gttatcggca
     9421 tcccgatgat tttggtggaa aacgtcatcg gacggcgcaa aggcgtgaac gcgctggatg
     9481 cgttcggcgg cccgatgaac ggcaaacctg ttgccaaaat ttggaaactg gtcggccgga
     9541 tggggctgct cggcgcgttc ggcatcatgg cttattatat ggtactcggt ggctgggtaa
     9601 tcagctatat cgttaatatt attggaggaa atttgaatat ttccagcccc gtcgacggtg
     9661 tggttacaaa aggcttcttt accgaacaca ttgaaaacag cccttgggaa attgcgtttt
     9721 atacgctgct ttttgtcgcc gtgaaccaat ggattttggt caaaggcgtt atcggcggca
     9781 ttgaaaaaac ggcgaaatac ctgatgcctc tgctgttttt gttcctaatc gcgatggtcg
     9841 tccgcaacgt tacccttccg ggcgcaatgg aaggggttgc tttctatctg aaacctgatt
     9901 tcagcaagat taccgccgaa ctgttcgtct tcgttttggg gcaggtattt tttgccctga
     9961 gcctgggttt cggcgtgatg attaccttat ccagctatct ggataaaaac gaaaatctgg
    10021 ttcagacggc agttatcacg gcaattacca ataccatcat cgctgtactt gcgggcttta
    10081 tgattttccc gtcgctcttc agcttcggcg tcgcgcccaa ttccggcccg actttggtgt
    10141 tccaaagttt accgattgtg ttctcacata tgtgggcagg acctgtgttc gccgtcattt
    10201 tcttctctct gctcctgatt gccgcgttga caacttcgct gacgatttat gaagtgttga
    10261 ttacgaccat tcaggaaaaa accaaaatcc gccgtaccgc cgcaatcacg attgtattgt
    10321 ccgtcatctt cgttttcggc aacatcccgt ctattttgag ctatggcccg tggaaagacg
    10381 tttccgtgtt cggcaaaaat attttcgatg ccttcgacta catcagcggc aacatcttgt
    10441 ttatgctgac cgcgctcggt tccgcattgt ttgtcggttt tgtgatgaag gacgaagcga
    10501 aggaagaatt gctttataaa ggcaaccata cgacggtcaa tatttggttt gcctatgtga
    10561 aataccttgt gccgctggtg attctgctga ttttcgtcag caacctgttc taatccgcag
    10621 caatcaatgc cgtctgaagg tcatacctct ttcagacggc attgcattga tgcccgccac
    10681 gccggaatcc gaaccggctt gccggcttct gtcggacgct ttccgggcag gcggcgttcc
    10741 gtctgcttag acaatcgtcc tttaaaacag gtagaatccg ccccaacggg aaacacaccc
    10801 ttcagacggc aaaacccata ccccaaacca tcaggaatcc cccttatgaa cagacaaaaa
    10861 gtcatcgcca tcgacggtcc gggcgcatcg ggcaaaggca cggtcgccgc ccgcgttgcc
    10921 gccgcattgg gatacgatta tctcgatacc ggcgcgctct accgcctgac cgccctatat
    10981 gcacaaaaac aaggcgtgga atggcacgat gaagaaaacg tttccgcact tgccaaaaaa
    11041 ctgcccgccg tattttcagg caaccgcatc ctacttgatg gcgaagacgt ttcagacggc
    11101 atccggacag aagccatcgg tatgggcgca tccgcagttg cgcaatggcc caaagtccgc
    11161 gccgcactgc tgcaacgcca acgcgatttt ctgaccgaaa aaggactggt tgccgacgga
    11221 cgggacaccg gatcggtcgt cttcccccaa gccgaactca aaatttttct gacagccgaa
    11281 tctaaaatcc gtgccgaacg ccgcgccaaa caaatcggca ttccctgcga aggtttcgca
    11341 ttcgagcgca tcctgtccga catcgagacc agagacgaag cagaccgaaa ccgcaaggtt
    11401 gcccccctga aacaacagcc cgatgccctg cttttggata cgagccgcct aactatagaa
    11461 gaaactgtaa aaaaagtgct tgattggtat cgtaaagttt aaattttcag gtataatcgc
    11521 ccgcattgcg cttcagacag catgaatttc catgtgccgt ctgaaacctt atttacccgc
    11581 ctgccgtgcc aaggactgca gacacccacc aacccaaccc gcaccccttg gcggtgtacc
    11641 gaaaagagtt atatatgtct atggaaaatt ttgctcagct gttggaagaa agctttaccc
    11701 tgcaagaaat gaacccgggt gaggtgatta ccgctgaagt agtggcaatc gaccaaaact
    11761 tcgttaccgt aaacgcaggt ctgaaatcag aatccctgat cgatgtagct gaattcaaaa
    11821 acgctcaagg cgaaattgaa gttaaagtcg gcgacttcgt taccgttacc atcgaatccg
    11881 tcgaaaacgg cttcggcgaa accaaactgt cccgcgaaaa agccaaacgc gcagccgatt
    11941 ggatcgcttt ggaagaagcc atggaaaacg gcaacatcct gtccggcatc atcaacggta
    12001 aagtcaaagg cggcctgacc gttatgatca gcagcatccg cgcattcctg ccgggttctt
    12061 tggtcgacgt acgtcccgtt aaagacactt cccattttga aggcaaagag atcgaattca
    12121 aagtgatcaa actggacaaa aaacgcaaca acgtcgttgt ttcccgccgc gccgttttgg
    12181 aagccacttt gggtgaagaa cgcaaagccc tgctggaaaa cctgcaagaa ggctccgtca
    12241 tcaaaggcat cgtcaaaaat atcaccgact acggcgcatt cgttgacctg ggcggcatcg
    12301 acggcctgct gcacatcacc gatttggcat ggcgtcgcgt gaaacacccg agcgaagtct
    12361 tggaagtcgg tcaggaagtt gaagccaaag tattgaaatt cgaccaagaa aaacaacgtg
    12421 tttccttggg tatgaaacaa ctgggcgaag atccttggag cggtctgacc cgccgttatc
    12481 cgcaagccac ccgcctgttc ggcaaagtat ccaacctgac cgactacggc gcattcgtcg
    12541 aaatcgaaca aggcatcgaa ggtttggtac acgtctccga aatggactgg accaacaaaa
    12601 acgtacaccc gagcaaagtc gtacaactgg gtgacgaagt cgaagtcatg attttggaaa
    12661 tcgacgaagg ccgccgccgt atctctttgg gtatgaaaca atgccaagcc aatccttggg
    12721 aagaatttgc cgccaaccac aacaaaggag acaaaatctc cggtgcggtt aaatccatta
    12781 ccgatttcgg cgtattcgtc ggcctgcccg gcggcatcga cggtctggtt cacctgtccg
    12841 acctgtcttg gaccgaatcc ggcgaagaag ccgtacgcaa atacaaaaaa ggagaagaag
    12901 tcgaagccgt cgtattggca atcgatgtgg aaaaagaacg catctccttg ggtatcaaac
    12961 aactggaagg cgatcctttc ggcaacttca tcagcgtgaa cgacaaaggt tctttggtta
    13021 aaggttccgt gaaatctgtt gatgccaaag gtgctgttat cgccctgtct gacgaagtag
    13081 aaggctacct gcctgcttcc gaatttgcag ccgaccgcgt tgaagacttg accaccaaac
    13141 tgaaagaagg cgacgaagtt gaagccgtca tcgttaccgt tgaccgcaaa aaccgcagca
    13201 tcaaactttc cgttaaagcc aaagatgcca aagaaagccg cgaagcactg aactccgtca
    13261 atgccgccgc caatgcgaat gccggtacca ccagcttggg cgacctgctg aaagccaaac
    13321 tctccggcga acaagaataa ggttgcagac atgacaaagt ctgagttaat ggttcgcttg
    13381 gcagaagtat ttgccgccaa aaacggcacg catcttctgg caaaagacgt agagtacagc
    13441 gtaaaagtct tggttgacac catgacccga tcgcttgccc gaggtcaacg catcgaaatt
    13501 cgcggtttcg gcagcttcga tttgaaccat cgtcctgccc gcatcggtcg caatcccaaa
    13561 accggcgagc gcgtggaagt acctgaaaaa catgtacccc acttcaagcc tggtaaagaa
    13621 ttgcgcgagc gggtcgactt ggctttaaaa gaaaatgcca attaaacctt agcatcaaaa
    13681 cgccgctggt acgcggcgtt ttttctttgg tttaacttca tccgctgctt caatacctta
    13741 agccaagcaa gcaacggatt agagcgtgga ttttttacca ttgcttacaa tccgcttttt
    13801 aaacaacaaa ttgttgattt ctattacgaa caggacaaaa tcctgcttat tgcactaaaa
    13861 ctaagccgtt tcaggaattt gcggcaaatt tacagctttt accgagccta atgctttcgc
    13921 tttttggtaa aacgccaatt tgtattcaag caaatctaaa taacgtttta attcggcaat
    13981 ttgacacttc acattttcta tttgattttc aaacaaggaa aggcgttctt caatggtatc
    14041 gtcgccaatg acggtacatt ccgcaaagcg tttgatgtct tttaagctca ttcccgtatt
    14101 tttcaagcat tgcaataagt acaaccattg caaatcgtta tcggtaaaac aacggttacc
    14161 gtattcatca cgtccgatat taggcaacaa gccttctttg tcgtaaaagc gtaaagtgtg
    14221 ggcggagatg cctatttttt cggcagcttt ggcagtggta taagtcattt tccctccctc
    14281 taaacaaaaa acggtaaaaa aacacttgcc ttagagtgaa ctctaaaatg taaactgttg
    14341 ctatgttgct caggcaaggc tattttgtta atgaattaag aggaaagaca atggaaatga
    14401 aacaaaccga ttcaaccatc aaatctcgtg cggcggtggc attcgcccct aaccaaccct
    14461 tacaaattgt ggaaatcgac gtggaaatgc cgcgtaaaga cgaggtgtta attcgtaaca
    14521 cccacactgg cgtgtgccat actgatgcat ttaccttatc aggaagcgat cctgaaggcg
    14581 tattccctgt ggtgcttgga cacgaaggtg cgggtgtggt cgttgctgtg ggcgagggtg
    14641 tgtcaagcgt aaaaccgggt aatcacgtca ttccgcttta caccgcagaa tgtggcgaat
    14701 gtgagttttg cctctcgggt aaaaccaatt tatgcgtctc agtgcgtgat acacaaggta
    14761 aaggcttaat gccagacggc acgacgcgtt tttcttatca aggtcagccg atttatcact
    14821 atatgggctg ttcgactttc agtgaatatt ctgttgttgc cgaagtttca ctggcgaaaa
    14881 tcaacccgaa agccaaccac gaacaagtat gtttgctcgg ctgcggcgtt accactggta
    14941 ttggtgcggt acataacacg gcaaaagtac aagaaggcga ttctgttgcc gtgtttggct
    15001 tgggagcgat tggtttggct gtggtgcaag gtgcgcgtca agccaaagcc ggtcgcatta
    15061 tcgccattga taccaatcct gcaaaattcg agttggcaaa acagtgttgg tgcaactgac
    15121 tgtttgagcc cgaaagatta cgataaacca atcaaagatg tgttgttaga catcaacaaa
    15181 tggggcattg accacacctt tgaatgtatc ggcaatgtaa acgtaatgcg tcaggcatta
    15241 gaaagtgcac accgaggttg gggacaatcc atcatcatcg gcgtagcgga tgcaggacaa
    15301 gaaatttcaa cgcgtccgtt ccaatcggta acaggccgtg tttggaaagg ctcggcattt
    15361 ggtggtgtta aaggccgctc cgagcttccg aaaatggtgg aggattcaat gaaaggcgat
    15421 atccaattag aaccatttgt gacccatgca atgacccttg atcaaatcaa tgaagccttt
    15481 gagttaatgc acgaaggtaa atcgatccgc gctgttattc attactaagg tccacgatga
    15541 aactgattga acaacatcaa atttttggtg gttcgcaaca agtttgggcg catcatgccc
    15601 aaacgcttca atgcgaaatg aaatttgccg tctatttgcc ggataatcgg gaaaatcaac
    15661 cgcttggtgt gatttattgg ctttccggct tgacgtgtac ggaacaaaat ttcattacca
    15721 aatcgagctt ccggcgttat gcggcagaac atcaagtggt tgtggtcgcc cccgataccg
    15781 gccctcgcgg agagcaagtg ccgaacggtg ccgcttatga tttaggacag ggggcaggct
    15841 tttatttgaa tgcgaccgaa cagccttggg cggcgaatta tcaaatgtat gattacattt
    15901 tgaacgagtt gccccgtctg attgaggagc attttcctac caacggcaaa cgttccatta
    15961 tggggcattc gatggacgga cacggcgcat tggtattggc actacggaat cgggaacatt
    16021 atcaaagtgt ttctgccttt tcgcctattt tatcgccaag cctcgtaccg tggggagaaa
    16081 aagcctttac tgcttattta gggaaagacc gtgaaaaatg gcagcaatat gatgctaact
    16141 cactcattca acaaggctat aaagtgcaag gtatgcgtat cgatcagggc ttggaagatg
    16201 agtttttgcc gacacaattg cgtaccaaag attttatcga aacctgtcgt gcggcaaatc
    16261 agccaatcga tgtgcgtttc cataaaggat acgatcacag ctattacttc atcgccagtt
    16321 ttattggtga gcatatcgct tatcacgccg catttttgaa gtaagtcaaa gagcggtcag
    16381 ttttcaaagc agtttggaat agccggcacg agggcggtaa gaagtgccgg cataaacgta
    16441 tgccgcctga accggaagga gccggcccta cggattacca aaccctgccc ttgatttgat
    16501 tagtatgatt tttaagctta aaccctgatt gatcaaacat taaaatcgcc cgtatttcct
    16561 acttcacttt cgggcttcgt attattcgtt gccgaatttg cataagcaac ctgttcctgt
    16621 tgtgttttcg ccccctccaa gctgacggtg gcgtaacagc ttcgcgattc accatctgtt
    16681 tcatttgtcc tccttcaata ttaaaacgca gcctgaaata aaaggcattt tggctcatat
    16741 ctgcaccata ttaaaacgcc gcctttgctt ataccccctt tgtgcgcgtc attattcttt
    16801 tccacgggaa acgccaagtt tgaaggaaat catttataat accagcggta agcattttct
    16861 ttcttagccg caagaagtat aacaaggtta aatatgaata atagagacca actttttaaa
    16921 gccccgccgt ttgaaaacca cagcccgctg acctggtatc aggctgcctc acaactgccc
    16981 aacttcatcc gcgacgacgc acaggcggcc gccatcgaac accttgaccg gctttggacc
    17041 gaattgatga tgttcaaacg caaaagaaac cgttttttag gcaggagttt gcgttccccg
    17101 caagttccca aagggcttta tttctatggc ggagtcggac gcggcaagag ctttctgatg
    17161 gacgcttttt tcggctgcct cccttaccgc cgcaaacgcc gcgtccactt tcatgccttt
    17221 atggcggaaa tccaccggcg gctgaaagcc ctgaaaagcg aaagcaaccc gttgaaatcc
    17281 gttgccgccg agattaccaa agaaacccga gtattgtgtt ttgacgaatt tcatgtcagc
    17341 gatattgcgg atgcaatgat tttaggccgt ctgctggaaa acctgcttaa cgagggcgtt
    17401 gttttggtgg cgacttcaaa ctacgcgcct tccgaactct acccgcaagg tcaaaaccgg
    17461 agcggttttc ttcccacaat cgcgctcatc gagtccagtc tgaccgtctt aaacgttgac
    17521 ggaggggaag actaccggct gcgtaccctc cgccccgccg agattttctt tacgcctgcc
    17581 aatgaagaaa atgaggcaaa actggcaaaa ctgttcaaag aaatgacagg cattaccgat
    17641 ttgaaccccg gcatcagcac catccacggc cgggaaattc cccacaaagc cgagtccggt
    17701 cgtaccatat ggtttgattt ccgcgcactg tgcttcagtc cccgctcaca gtccgactat
    17761 ctgtatttgg ccgaacatta tgaaatggtt tttatttcag gtttggaaca actctcaccg
    17821 caagaaaagg cggaggcgcg acggctgact tggctgattg acgtactcta cgatttccgg
    17881 gtcaaactgt gtgccaccgg cgcggtagat gtcaaccata tctacacgga aggcgatttt
    17941 gccgaagaat ttacccgcac cgccagccgg atggtcgaaa tgcagtccga agtttatttg
    18001 gaacagccgc acctgacctt atctcccaag gcttcaggcg gataagttat ttttttggta
    18061 gaataccgat ttgattcttc ttaagtaaaa ataaggatat agcatggcga ttgaacgtac
    18121 catatccatc atcaaacccg atgccgtcgg caaaaacgtt atcggcaaaa tatacagccg
    18181 ctttgaggag aacggtctga aaatcgttgc cgccaaaatg aagcagctta ccctcaaaga
    18241 agcccaagaa ttttatgcgg ttcataaaga ccgccccttc tatgccggat tggttgaatt
    18301 tatgaccggc ggtccggtta tgattcaagt actggaaggc gaaaacgccg ttctgaaaaa
    18361 ccgcgaactg atgggggcga ccaatcccac cgaagccgca gaaggcacga tacgcgcgga
    18421 ctttgccact tcggtcagca ttaacgccgt acacggttcc gacagtgtgg aaaatgccgc
    18481 tttggaaatt gcctactttt tcagccaaac cgaaatctgc ccccgttgat acaatacacc
    18541 gcccaattcc tattcagacg gcataaaata tatccatgcc gtctgaaaac tctgttgcaa
    18601 aagacttcaa atcaaacttg cctgccctgc aattttttat ttgaagcctt aatttaagaa
    18661 aaacacaaat acatgaaaac caatctgctc aactacgacc ttcaagggct aacccgacat
    18721 tttgccgata tgggtgaaaa accattccgc gccaaacagg ttatgcgttg gatgcaccaa
    18781 tccggcgcgc aaaattttga cgaaatgacc gatttggcaa aatcgttgcg ccataaactg
    18841 aacgaacagg caagcatcga aattcccaag ctgatgatgt cccaagaatc ttcagacggc
    18901 actcgaaaat ggcttttgga tgtcggtacg gggaacggcg tggaaaccgt cttcatcccc
    18961 gaatcggatc gcggcacgct ctgcatttcc tcgcaagtcg gctgcgcttt ggaatgtaca
    19021 ttttgttcga ccggccggca gggcttcaac cgcaatttaa ccgctgccga aatcatcgga
    19081 cagttgtggt gggcaaacaa agcgatgggc gttacaccga aaaacgagcg tgtgatttcc
    19141 aacgtcgtca tgatgggcat gggcgagccg atggcgaact tcgacaatgt cgttaccgcc
    19201 ttgagcatca tgctggacga ccacggctac ggtttgagcc gccgccgcgt aaccgtttcc
    19261 acttcgggta tggttcccca aatggacagg ttgcgcgatg tcatgccggt ggccttggcg
    19321 gtttccctcc acgcttccaa tgacgaagtc cgcaaccaaa tcgtaccgtt gaacaaaaaa
    19381 tatcccttga aagaattgat ggccgcatgc caacgctatc tggtcaaagc gcccagggat
    19441 ttcatcactt tcgagtatgt gatgctggac ggtgtcaacg ataaggcaca acatgcatac
    19501 gaacttattg aactggtcaa agatgttccc tgcaaattca atttaatccc gtttaatcct
    19561 ttccctaatt cgggatatga acgttcaagt aacgaaaaca tccgtatttt cagagatatc
    19621 ctgcaacaag ccgaatttgt cgttaccgtt cgaaaaactc gtggcgacga tatcgatgcc
    19681 gcctgcggac agttggcagg tcaggttcag gataaaacgc gccgccaaca aaaatggcag
    19741 cagattttaa tcggacaaca ggggtaatta tgccttttaa gccatccaaa cgaatctctt
    19801 tattacttgt tcttgccttg ggcgcgtgca gcacttccta ccgcccctcg cgggcagaaa
    19861 aagccaatca ggtttccaat atcaaaaccc agttggcgat ggaatatatg cgcggtcagg
    19921 actaccgtca ggcaacggca agtattgaag atgccttgaa atcgaaccct aaaaacgagc
    19981 ttgcctggct ggtccgtgcc gaaatctatc aatacctgaa agttaacgac aaggcgcagg
    20041 aaagtttccg gcaagccctc tccatcaaac ccgacagtgc cgaaatcaac aacaactacg
    20101 gctggttcct gtgcggcagg ctcaaccgcc ctgccgaatc tatggcatat ttcgacaaag
    20161 ccctggccga ccccacctac ccgacccctt atattgccaa cctgaataaa ggtatatgca
    20221 gcgcaaaaca ggggcaattc ggattggcgg aagcctattt gaaacgttcc ctcgccgccc
    20281 agccgcagtt cccacccgca tttaaagaac tggcgcgcac caaaatgctg gccgggcagt
    20341 tgggcgatgc cgattactac tttaaaaaat accaaagcag ggtagaagtc cttcaggccg
    20401 atgatttgct gctaggctgg aaaattgcca aagccctcgg caacgtgcag gcggcatacg
    20461 aatatgaagc acaattgcag gcaaatttcc cctactcgga agaattgcaa accgtcctca
    20521 ccggtcaata aacagattca aactatatga acacactcca acgccgcaag acgcatcaag
    20581 tcctcatcga tcatattacc gtcggttcag aagcacccgt cgttatccaa tctatgacca
    20641 acaccgacac tgccgatgca aaagccaccg cattgcagat taaggaattg agcgatgccg
    20701 gctcggaaat ggtgcggatt accgtcaaca gccccgaagc cgcgtccaaa gttgccgaaa
    20761 tccgccgccg cttggacgat atgggctatg ccacaccact gattggcgat ttccatttta
    20821 acggcgaacg cctgttggcg gaatttcccg aatgcggcaa agcattgtcc aaataccgca
    20881 tcaatcccgg caatgtcggc aaaggcgtaa aaggcgatga aaaatttgcc tttatgattc
    20941 ggactgctgc tgaaaacgat aaagccgtcc gtatcggcgt aaactggggt tctttggatc
    21001 agagcctcgc caaacgtatg atggatgcca acctcgtttc ttccgcgccg aaaccgcccg
    21061 aagaagtgat gaaggaagca ctgattgtct ccgctttgga atctgccgaa aaagccgttc
    21121 tattgggact gcccgaagac aaaatcatcc tgtcgtgcaa agtcagcgcg gttcatgatt
    21181 tgattcaggt ttaccgcgaa ctgggcagcc gttgcgtcta tccgctgcat ttgggtttga
    21241 ccgaagccgg tatgggcagc aaaggcattg tcgcatcaac ggcggcatta tctgttttgc
    21301 ttcaagaagg aattggcgac accatccgca tttcactcac tccggaaccg ggcagcccgc
    21361 gtactcagga ggtcgtcgtc gggcaagaga ttttacagac tatgggtttg cgctcgttta
    21421 cgccgatggt taccgcctgc ccgggatgcg ggcgtaccac cagtaccgta tttcaagagc
    21481 tggcacaaga tgttcaaaat tacctgcgcc aaaaaatgtc tatatggcgt accctttatc
    21541 ccggggttga atccctgaac gttgccgtaa tgggctgcgt tgtcaatggc cccggagaaa
    21601 gcaaattggc cgacatcgga atcagccttc ccggtacggg ggaaacaccc gtcgcacctg
    21661 tttatgtaga tggggagcgc aaagtcacac tgaaaggtaa taatattgca agcgaattcc
    21721 tagctattgt agaggaatat gttaaaacca attatggcaa aaacagttct aaacgcaata
    21781 aaggaaaagt catcccgata cagtctctat aaaacaaatg ccgtctgaac gtttcagacg
    21841 gcatttgttt ctctcagatt tcttattaaa gccgcaaaga agcgcggttt tccaaaactt
    21901 ggtcgatcaa accatattct tttgcttctt cggcagacat gaaattatca cggtcggtgt
    21961 cgcgctccaa atctgccaaa tcgcggccgc aatgtttcgc catcaggcgg ttgagttttt
    22021 ctttgatttt caacaactcg cgtgcgtgga tttcaatgtc ggatgcctga ccgcccaagc
    22081 cgccgctgat taaaggctgg tgaatcataa tccggctgtt gggcagggcg aaacgtttgc
    22141 ctttctcgcc tgccgacaat aagaacgcgc ccatacttgc cgcctgcccc aagcacaaag
    22201 tcgatacatc gggcttgatg aaattcatcg tgtcgtaaat cgacataccg gccgttaccg
    22261 agccgccggg ggaattgatg tagaagaaaa tatccttatc cggattctca ctttccaaaa
    22321 acaacagttg ggcgaccacc agattagcag actcatcggt taccgggcca accagaaata
    22381 cgatgcgctc tttcaaaagc cgggaataga tatcgaatgc acgctcaccg cgaccgctct
    22441 gctcgataac ggtagggaca agatggttat caaaagacat ttcgtctcct ttcatgatgg
    22501 aaaagcacca aagcgggctt taaaaacggc ttcggtgctt tcaaaaactg ccttcagaca
    22561 gcattttcag aacaatcagg cttgcgcgcc catcacttcg tcaaaagaca aagctttttt
    22621 gtttactttg gctttgccca aaacgaaatc aacaacgttg ctttctactg ccaaagaagt
    22681 cggggcttgc aggcgggaag tatctgcgta gtaccagtca atcacttctt gaggatcttc
    22741 gtagctttct gcgaagttgg cgacaacggc tttgatttgc tcttcagtcg gttccagttt
    22801 gttttcgtca accagtttgg ccaaaatcag acccaaagat acgcgacgtt cggcttgttc
    22861 tttgaacata tccaaaggca aatccaagtt cgcggcatcg gtcatacctt ggttaacgaa
    22921 gttttgtttc atttcgtttg ccaggcgggc ggcttcttca ttgaccaaag caaccggaac
    22981 tttcaactct acggctttaa tcagcgcgtt cattacggat tctttggttt gttcgttcac
    23041 gcggcgttcc acttcgcggc ttacgttttt cttcacttct tcacgcattt tggcaacatc
    23101 gccatccgca atacccaagg cttttgcaaa atctgcatcg acttcaggca gagtgggctc
    23161 ggaaacattg ttcagcgtaa tggtgaacac ggcagtttta ccggcaacat ctttgccgtg
    23221 gtattcttca gggaagttga cggtaacgtc tttactttcg cccgctttca tgccgactac
    23281 gccggcttca aattcaggca gcatttgacc tgcgcccaat acgaaggcgt agtttttgga
    23341 tgcgccgccg gcaaaaggtt cgccgtcgat tttgccttca aagtcgatga tgacgcggtc
    23401 gccgtttcgg gcttcgcggt caacatggtt gaagcgggta cgttgtttac gcaggatttc
    23461 tacagtttgg tcgacttcgg catcgccgac ggaagcggtt actttttcga cctcttgtgc
    23521 agacaaatcg ccgataacga cttcggggaa cacttcaaaa atggcggcaa ctttgaaaga
    23581 ctctttatcg tcttgttctt caacgccttc aaaacgtgga tagcctgcca ctttcaactc
    23641 ttgggcaacg gcaacatcgt agaagcggcg ttgcaccagc tcgttgatca cgtcgttttg
    23701 cgcgctcgca ccgtacattt gggcaatcat ttttaaaggt gctttacccg gacggaaacc
    23761 gtcgattttt gcacggcgtt gggtttgttt cagtttttta tcggtttctg cgttgatttc
    23821 ggaccaaggc agggacaaca ctactttgcg ttccagattt tctaaaattt caacagttac
    23881 gctcatcata agcccttaaa tttgttgtgt tgataaaatg ataaaccttc ttccctacat
    23941 ggggaagcaa acagcgcaac ggtacgatat ttgaaccgca ttgccgcaaa ggggaaattt
    24001 tagctggcaa gtatatcaca atgtttcgcc tgaaacataa tatgccgaga cctttgcaaa
    24061 attcccctaa aatcccctaa attcccacca aggcatttag gggatttccc atgagcacct
    24121 tcttccggca aaccgcccaa gccatgactg ccaaacacat cggccgcttc ccgctatcgg
    24181 agttggacca
//
LOCUS       DAXFZR010000025        23824 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000025 DAXFZR010000000
VERSION     DAXFZR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23824
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.025"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..158
                     /locus_tag="ACFJAS_000850"
                     /pseudo
     CDS             1..158
                     /locus_tag="ACFJAS_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(200..370)
                     /locus_tag="ACFJAS_000851"
     CDS             complement(200..370)
                     /locus_tag="ACFJAS_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC3982140.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            complement(503..1594)
                     /locus_tag="ACFJAS_000852"
     CDS             complement(503..1594)
                     /locus_tag="ACFJAS_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC3982141.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIGRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLAGIEKHNLQAECVDGGYKVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQREGVSLQNCPEFCALEGTHTFSLNFKDVFIPDEDIIAAPE
                     QFAGYIQSIKAGFILLQIGIGAGVIDGSLGIIRLVNVVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVGEAMFVAIVTPAIKHLCKEIAAIEAAK"
     gene            complement(1729..2277)
                     /locus_tag="ACFJAS_000853"
     CDS             complement(1729..2277)
                     /locus_tag="ACFJAS_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC3982142.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVLKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAVRTNQCSFCVAGHTKPATLKKLLSEQSVKAA
                     RALDAGAFDDAKLGALAAFTQAVMAKKGAVSDDELNAFLEAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPKLQAYA"
     gene            complement(2417..2788)
                     /locus_tag="ACFJAS_000854"
     CDS             complement(2417..2788)
                     /locus_tag="ACFJAS_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC3982143.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAMFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            complement(2973..4664)
                     /gene="dld"
                     /locus_tag="ACFJAS_000855"
     CDS             complement(2973..4664)
                     /gene="dld"
                     /locus_tag="ACFJAS_000855"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC3982144.1"
                     /translation="MSASQLLSSLTQITGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNGCMALEHEM
                     LHLLEQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            complement(4839..4935)
                     /gene="ffs"
                     /locus_tag="ACFJAS_000856"
     ncRNA           complement(4839..4935)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFJAS_000856"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5196..9029
                     /locus_tag="ACFJAS_000857"
     CDS             5196..9029
                     /locus_tag="ACFJAS_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC3982145.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWHILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQADVLIAAAEKAVERFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRSVEYVELAGLYGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTAATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            9106..10106
                     /locus_tag="ACFJAS_000858"
                     /pseudo
     CDS             9106..10106
                     /locus_tag="ACFJAS_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
     gene            complement(10167..10349)
                     /locus_tag="ACFJAS_000859"
     CDS             complement(10167..10349)
                     /locus_tag="ACFJAS_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982146.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(10522..10653)
                     /locus_tag="ACFJAS_000860"
     CDS             complement(10522..10653)
                     /locus_tag="ACFJAS_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982147.1"
                     /translation="MSETAKIKLNDRPENAEKPNEKVELPIVDNDKKGGHGEGGCCG"
     gene            complement(10912..12056)
                     /locus_tag="ACFJAS_000861"
                     /pseudo
     CDS             complement(10912..12056)
                     /locus_tag="ACFJAS_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(12380..12856)
                     /gene="greA"
                     /locus_tag="ACFJAS_000862"
     CDS             complement(12380..12856)
                     /gene="greA"
                     /locus_tag="ACFJAS_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC3982148.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            complement(13120..13248)
                     /locus_tag="ACFJAS_000863"
     CDS             complement(13120..13248)
                     /locus_tag="ACFJAS_000863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982149.1"
                     /translation="MAKRKYKDKNSLHYTGANAVWKISGLAFSVLKYVNLVFYAEN"
     gene            13247..14548
                     /gene="aroA"
                     /locus_tag="ACFJAS_000864"
     CDS             13247..14548
                     /gene="aroA"
                     /locus_tag="ACFJAS_000864"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC3982150.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHIGKRQDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(14626..15159)
                     /locus_tag="ACFJAS_000865"
     CDS             complement(14626..15159)
                     /locus_tag="ACFJAS_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC3982151.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(15216..16742)
                     /locus_tag="ACFJAS_000866"
     CDS             complement(15216..16742)
                     /locus_tag="ACFJAS_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC3982152.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(16854..18395)
                     /locus_tag="ACFJAS_000867"
     CDS             complement(16854..18395)
                     /locus_tag="ACFJAS_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFC3982153.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSGYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            18543..19322
                     /locus_tag="ACFJAS_000868"
     CDS             18543..19322
                     /locus_tag="ACFJAS_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC3982154.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            19319..20020
                     /locus_tag="ACFJAS_000869"
     CDS             19319..20020
                     /locus_tag="ACFJAS_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC3982155.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            20017..21471
                     /locus_tag="ACFJAS_000870"
     CDS             20017..21471
                     /locus_tag="ACFJAS_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC3982156.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIVITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(21620..22039)
                     /gene="fitB"
                     /locus_tag="ACFJAS_000871"
     CDS             complement(21620..22039)
                     /gene="fitB"
                     /locus_tag="ACFJAS_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFC3982157.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(22039..22275)
                     /gene="fitA"
                     /locus_tag="ACFJAS_000872"
     CDS             complement(22039..22275)
                     /gene="fitA"
                     /locus_tag="ACFJAS_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFC3982158.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
     gene            complement(22480..22758)
                     /locus_tag="ACFJAS_000873"
     CDS             complement(22480..22758)
                     /locus_tag="ACFJAS_000873"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3982159.1"
                     /translation="MAISDVFYQKQLKGNGLVQSLSRKGNCLDNAAMESFFGTLKSEC
                     FHTCKYDSVTESEAALHEYIRYYNNDRIKLKLKGLSPVQYRIQSLKAA"
     gene            complement(22722..23300)
                     /locus_tag="ACFJAS_000874"
     CDS             complement(22722..23300)
                     /locus_tag="ACFJAS_000874"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC3982160.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAQNI
                     LQRCFHSEKPNEKWVTDVTEFDVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPGPSEKPMLHSDQGWQYQMFFIKSS"
     gene            complement(23305..23706)
                     /locus_tag="ACFJAS_000875"
     CDS             complement(23305..23706)
                     /locus_tag="ACFJAS_000875"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3982161.1"
                     /translation="MARYRLHGESGIKRRKHTTKYSVEYKLEAIRPVTGQGMSQKAAA
                     DQLNLPECSVLPQWLRLYRLNGINGLKPKPKGRKPVKKQYPPQTKKADYLKTKEELFA
                     ELAYLKAEAAVLKKLDALKEVRQKERNLSQG"
BASE COUNT         5490 a         6367 c         6448 g         5519 t
ORIGIN      
        1 cgcattattt gacacggccg gccggatttt ccgactgcca acgcgtttgt ccggatgaaa
       61 caggattcga ccgccgcctg ttccgcccct atgcccgcag cctgaaaggg caaatggcga
      121 aagcgcggat aagtgggaaa agataccggc gcttatctgc ggaacacgcc gagggatttt
      181 ccaaggggtg cggctgccgc tatatcacga aatccaacag gtagaaatct tctttgccca
      241 cgccgcattc ggggcatttc cagtcgtcgg ggatgtcttc aaactttgtc ccgggggcga
      301 tgccgtgttc cgggtcgccg agttcttcat cgtaaatcca gccgcagggg ccgcacatat
      361 attgagccat ttgtgtttcc ttgtttgtac cgtgcttttt tgcagcgggc cgccggggtt
      421 tggttcggat gccgtctgaa gcgtgccgcc gcatctgtcc cgccttgggc ggtttgtgct
      481 tcagacggca ttttccgcct gtttattttg cggcttcgat tgctgctatt tctttgcaga
      541 ggtgtttgat tgcgggggtt acgatggcga caaacatcgc ttcgccgacg cgcctttggg
      601 cgggactccg catcaggtag cctttcgcgc cggaatgcag ggcggcggat tgcgcagcgg
      661 caagggccag tacggcggcg gcttcgcgca gcttgagggt ggcgaggttg tcgggcgtgt
      721 tgtcccacgc caatccggcg agccgttcgg tttctgccca cgcgccgtcc agccttgctt
      781 tgaggctgtc gtagccgtcg tcgagatagc tgttgacttc tgcattgacg acgttgacga
      841 ggcggatgat gccgaggctg ccgtcaatca cgcccgcgcc gataccgatt tgcaggagga
      901 tgaagcctgc tttgatgctt tggatgtagc cggcaaactg ttcgggcgcg gcgatgatgt
      961 cttcgtcggg aataaatacg tctttgaaat tcaggctgaa ggtgtgcgtg ccttcgaggg
     1021 cgcaaaattc ggggcagttt tgcaggctta cgccttccct ttgcccgcct gtgatgaaca
     1081 tgacgtagcc gccgccgatt tgggcggtat tcgcccaaat gtggtcttcg ccgatgttgg
     1141 acacccacgg cagcgcgccg ttgactttgt aaccaccgtc cacgcactcg gcttgaaggt
     1201 tgtgtttttc aatgccggca aggtgtttga cggtattgga cattccggtg cccgccaata
     1261 ctttgccttg caggatgtcg gcgaggtatt tgtctttgac ggcttggttg ggcgtttggt
     1321 gcagatacca cgcgcaagcc gcctgacacc acgcgctgaa ggaggtcgcg ccgcattctt
     1381 tgccgatttc gcgcaagacg gcgattcgtg ttgccaagcc taagccgttg ccgccttcgg
     1441 cttctgtacc gactgcgccg aatccgccga ttgcgccgag ttcgcgcata aatgcttcgg
     1501 ggtagtatcc tttgcggccg atgtcgtcca ctatgggttt gagcttggtt ttgacgagtt
     1561 cggcaacgtt ggcaatcagg gtttgggcgt tcatctttgt tccttaaggt ttacggggga
     1621 atcgggggcg cgcctgatgc gcggctgctt ttcctgccgc ttggcggctt gtttttgttt
     1681 gagggggtgt ttttgaaaaa ccgcctgcat tcaggcggtt ttgtttgctt aggcgtatgc
     1741 ctgcaatttg gggttgattt cggtttgggc gaggttgttg gcgtagttgc acaaagttgc
     1801 caaggctacg cccatcacga cttcgaccgc ctgctgccgg ttgtagcccg cttcgaggaa
     1861 ggcgttgagt tcgtcgtcgg atactgcgcc ttttttcgcc atcacggctt gggtgaaggc
     1921 ggcaagcgcg ccgagtttgg catcgtcaaa cgctccggca tcgagcgcgc gggcggcttt
     1981 gacggattgt tcggatagga gttttttcag ggttgccggt ttggtgtgtc ctgccacgca
     2041 gaagctgcat tggttggtgc ggacggcgat gatccggatc acttcgactt cgccggcggt
     2101 caggctgttg gcggcgttga gcttgccgac ttcttggtaa aacgccaaag cttcgggggc
     2161 gtttgccaat acgccgataa ggttggggat aaagccgttg tttttgagta cggcctctac
     2221 gcgcggtttg gcggcttcgg gggcggtttc gagggtgtgt acggttaaac gtgccatttt
     2281 ctttccttgt tcgcaaatat tggggacggg cgcatggtac gcatttcgga acggaatagg
     2341 aaagactgtg ccacaaacaa aaagttataa gaaatgccgc ctgaagcctt tcagacggca
     2401 ttgccgggaa acacgcctac ccgcccccgc gtccgaaata ttgcgccaat gcggcttggg
     2461 ggttggcggc ttcgactgtt tcgggcaatt cggtatagcc gcgcgataaa aggtggcgtg
     2521 cataaaggct gttgcccttt gcgccgaacc agacggaaac cgccaagccc aatacgttaa
     2581 acgccgagtc tgtggcgggc gtgtcgccgt aaaccagttg ggcaaacacc gccaacacga
     2641 acatcgcgcc cgtcatcccc agccccaaac cccacattct tttgaaacac gcccacagcg
     2701 tgccgaacaa aagacccggc cacgaccagc cttgtttgac ggcttggggc ggcagggcgg
     2761 gatgggtgta gattttgtat ggtttcatcg tgtttccttt tcggttgaaa ccccgccctt
     2821 tggggcggta ggatcagact ttatttgtga gacgcgcgcc cgtgtgccga catacggctt
     2881 gaacatgccc gtcggggaag acagatccga caatgccgtc tgaacggtgt tttgccttca
     2941 gacggcattg cctgccttca aagcggacgc gcttattccg cccagttttt ctttttgctg
     3001 gttttgccga tacccgggtt gaagctgttg gtcgggtcga gtttgcggta aaactgtttg
     3061 agcgcgggct tggcttcata caaatggccg acgttgtgtt cggcgggata ttgcgcgccg
     3121 cgttgctcta agagatgtag catttcgtgt tccaacgcca tacagccgtt gccttttttg
     3181 atgatgtaat cctgatggaa aacgtggcac ataaaatgcc cgtaatacaa tttatggatg
     3241 attttattgt cgatttccgg cggcagtttt tcaaaccagt cgcggtcgtc gcggcgcagg
     3301 gcgatatcca gcgcgaccaa gtcctccact tcgtcgtcgt gcacggaacg gtagcggatg
     3361 gcggcggaag cgacggcgaa acggtgcagc atcgcggctt gggtttcttc ggcgttgcac
     3421 tcgaaaaacg cgccgccgtg gtgtgaaaaa tattctttta agaacgcgcg cgcctcatcc
     3481 acgccttttc cgcccatttt cagaatcagg tggtgttcgt atttgtcgcg gtaatcgcgc
     3541 atggatttgg gcaggtggtc gggcaggaat ttgctgacga actgcattgc cttgtcggaa
     3601 aaatgtttgg gcaggaagct gacttttttg ccgaacctgt ccacgcgcgc cttcaagtca
     3661 aataatttcg gcagttggtg cgtaccgaat tttttgatga cgtagaacgt gtctttgccg
     3721 tacacgtcgg caatgtcgaa agcgtggcgg tggatgtatt cgccggaaac gggcaggctt
     3781 tcaaattcgc ccaaggcggc gcggcggatg tcggtcagct cgttgatgtc gttcgtgccg
     3841 atgtagaaca cggcggtttg tttttcttgc gggaaggtgt ccaaacggac ggcaaaaacc
     3901 atcagcttgc ccgcgcagcc cgaggcttcg taatggcggg cggggtcggc attgaaacgc
     3961 gcggcggtcg gttcgtccac ttggcggacg tgttcgcaat aggcgtggtc atgtcctttg
     4021 cccgcgtctt gtttgatgtc ttttttctga taatgatgac cttgcaggtt ggtcaggatt
     4081 tcttcgggcg tgtcgcccaa gtctatgccc aagtggttga ccagttccaa cctgccctct
     4141 tcgttgattt gggcgaacaa cgccatttcg gtgtaggccg ggccgcgctg taccaacgcg
     4201 ccgccggagt tgttgcacac gccgcctaaa acggacgcgc cgatgcaaga agagccgatg
     4261 accgaatgcg gttctcgtcc caaaggtttc agcagcagtt caagctggtt cagggtcgag
     4321 ccgggcaggc agacgacttg ttcgttgttg ttgatggttt ggatgatgtt catccgcatg
     4381 gtgttcacaa tcacgatgtc gcggtcgtaa tcgttgccgt cgggggtcga accgccggtc
     4441 agtccggtat tcgccgcctg cgtaatcaca atcacgtccg cttcgacgca ggcctgcaga
     4501 attttccaca tttccaaaat gcttcccggg cgcaccaccg ccaacgcctt gccttcgccg
     4561 aaacggtagc cttggcggta ttgttcggtt ttcgcggggt cggtgatgat gtatttttcg
     4621 cctgtgattt gggtcaggct tgacagtaat tgtgatgcgc tcatggcagt ttccttaaaa
     4681 ttgtcggcag gtgcatatgc acattggaat tgttttcaca ttgtagttat acgttatggc
     4741 aaagtaaaga aaatgccgtc tgaaagggcg ttatcgtata cgctgcggcg acataaagga
     4801 aaacgccgga attttcagaa ttccggcgtt tcaaataagg cggcgggcca aacccccggc
     4861 actggcgcat cggagtgggc tgctggcttc cgcccctgac ccggtgttcc gatttgccat
     4921 gcggggagac ccgcctcaga gaaacggcat tataacgggt tttctgaaaa actcaaccgt
     4981 tttgatacgg tcatacgccg gaaacaccgc ctaaaattta tatttgataa tattgtcaac
     5041 aatttctcaa agcgttattt tgtttctata agggtatttc ctgtttcggc attgaaaaat
     5101 atcaaaaatt gaactacatt atcgcctttt caaacccggc tgaaaccgac ttttcaaacg
     5161 gcattcaaat aaaaactgcc aaacacggac acaccatgac cacgactacc gccccgcagc
     5221 gtattcggga aatcccctac aactatactt cctacaccga ccgcgaaatc gtcatccgcc
     5281 tgctgggcga cgaggcgtgg cacattctgc aggacttgcg cggacagcgc aaaaccgggc
     5341 gttcggcacg gatgctgttt gaagtgttgg gcgatatttg ggtggtcgta cgcaatccgt
     5401 atctggtcga tgatttgctg gagcatccga aacgccgcgc cgcgctggtg cgggaaatgc
     5461 gccacaggtt gaacgaaatc cgcaaacgcc gcgacgataa tcagcaagcg gatgtgttga
     5521 ttgcggcggc ggaaaaggca gtggagcgtt ttgacggcag ctttgacgaa acgcgccaaa
     5581 agcggcggca gattttggag cgtttgagca aaattaccaa gccgcacaat attatgtttg
     5641 acgggctggc gcgggtaaca cacgttaccg atgcgaccga ctggcgcgtc gaatatccgt
     5701 ttgtcgtcgt caatcccgac acggaggcgg aagtcgcgcc tttggtgcgc gccttaatcg
     5761 agctggattt ggtcattatc ccgcgcggcg gcggtacggg ttataccggc ggcgcggtac
     5821 ctttggacgc aaacagcgca gtcatcaata ccgaaaaact cgacaagcat cgcagcgtcg
     5881 aatacgttga gctggcaggc ttgtacggca gacatccgat tatccggtgc ggcgcgggcg
     5941 tggttacgcg gcgggtggaa gaaaccgcgc atcaggcggg cttggtgttc gccgtcgatc
     6001 cgacttccgc cgacgcgtcc tgcgtgggtg gtaatgtggc gatgaatgcg ggcggtaaaa
     6061 aagccgtatt gtggggtact gccttggaca acctcgccta ctggaacatg gttaaccctc
     6121 aaggcgaatg gctgcgtatc gaacgcgtgc gccacaattt cggcaaaatc cacgacgaag
     6181 aaactgccgt gttcgacgtg cacacgctgg attcagacgg catcaacatc gttaaaaccg
     6241 aacgcttgga aatccccgga cacaaattcc gcaaagtcgg tttgggcaaa gacgttaccg
     6301 acaaattttt gagcggactg cccggcgtgc agaaagaagg tacggacggc atcattaccg
     6361 gcgttgcctt cgtattgcac aaaatgccga aatacacgcg caccgtgtgt atggagtttt
     6421 tcggcacggc cgccaccgcc acgccttcta tcgtcgaaat ccgcgatttt ctgctcgcac
     6481 acgacagcgt gcggctggcg ggtttggagc atttggactg gcgttatgtc cgcgccgtcg
     6541 gctacgccac caaagcggcg ggcaaaggac gaccgaaaat ggtcttgctg gcggacgtgg
     6601 tttcagacga cgaagccgcc gtagaggcag ccgccgaaca catctgcgaa cttgcccgcg
     6661 cccgtgacgg cgaaggcttt atcgccgtat cgcccgaagc gcgcaaaacc ttttggctcg
     6721 accgcagccg caccgccgcc atcgccaaac acaccaacgc ctttaaaatc aacgaagacg
     6781 tggtgattcc gctggagcgg ctgggcgagt attcggacgg catcgaacgc atcaacatcg
     6841 agctttccat ccaaaacaaa ctcaaactct gtgccgcttt ggagcaatat ctttcgggca
     6901 aactccccat cgacaaaatg ggcaccgacc tgccgaccgc cgaactgttg ggcgaacgcg
     6961 gcaaacacgc cctcgcccac gttgccgccg tcaaagcgcg ttgggactgg ctgctcgccc
     7021 atttggatgc gccgcttgcc gactacaaat cacgctacgg tacagccgtc cacgccgcgc
     7081 ccgaagccaa agacgatgaa agctgcttca ccgccttccg cgatttccgc ctgcgcgtgt
     7141 ctgtcaaagc agacgtaatg aagccgcttg ccgaaatctt cagcggcaaa accgatacca
     7201 aaattatcca aggcttgggc aaaatccacg ccaaaaccgt acgcagccgc gtctttgtcg
     7261 ccctgcatat gcatgccggc gacggtaacg tccacaccaa tattccggtc aactcagacg
     7321 atgccgaaat gcttcagacg gcctaccgtt ctgtggaacg cattatgaaa atcgcccgtt
     7381 cgctcggcgg cgtgatttcc ggcgaacacg gcatcggcat caccaagctc gaattcctca
     7441 ccgatgaaga tttgcaaccg ttttggaact acaaaaacca agtcgacccc aagcacacct
     7501 tcaaccgcca caaactgatg aaaggttcgg acttacgcaa cgcctacacg ccgtctttcg
     7561 agctgttggg cgcggaatcg ctgattatgg aaaaatcaga cctcggcacg attgccgatt
     7621 ccgtcaaaga ctgcctgcgc tgcggcaaat gtaaacccgt ctgctccacc cacgttccgc
     7681 gcgccaacct gctgtacagc ccgcgcaaca aaatcctcgg cgtgggtttg ctgaccgagg
     7741 ccttcttata cgaagaacaa acccggcgcg gcgtttccgt caaacatttt gaagaactga
     7801 tggacatcgg cgaccactgc accgtgtgcc accgctgcgt caaaccctgc cccgtcaaca
     7861 tcgacttcgg cgacgttacc gtagccgtcc gcaactacct tgccgattcc ggccacaaac
     7921 gctttgcgcc tgcggcttca atgggtatgg cgtttttgaa cgccaccggt ccgaaaacca
     7981 tcaaagccct gcgcgccgcg atgatacaga ccggcttccc cgcgcagaac tttgcctaca
     8041 aaatcggcaa gcttcttccg atcggcacga aaaagcaaaa agccgaaccc aaggcaaccg
     8101 tcggcaaagc cccgattaaa gaacaggtta tccatttcat caaccgccca ctgcccaaaa
     8161 gcgtacccgc caaaacaccg cgctccttat tgggcatcga agacggcaaa agcatcccca
     8221 tcatccacaa ccccgccgcg cccgaagatg ccgaagccgt gttctacttc cccggctgcg
     8281 gttcggagcg tttgttcagc caaatcggac tcgccgtcca agccatgctc tggcacgtcg
     8341 gcgtacaaac cgtcctgccg cccggctata tgtgttgcgg ctatccgcaa gacgctggcg
     8401 gcaacaaggc aaaagccgaa gaaatgagca ccaacaaccg cgtcgccttc caccgtatgg
     8461 cgaacaccct caactacctc gacatcaaaa ccgtcgtcgt cagttgcggc acttgttacg
     8521 accagcttga aaaataccgc ttcgaggaaa tcttccccgg ctgccgcatc atcgacatcc
     8581 acgaatacct gctcgaaaaa ggcgtgaaac tcgacggcgt gaaaggtcag caatacctct
     8641 accacgaccc ctgccacacc ccgattaaaa ccatgaacgc cacccaaatg gccagcagcc
     8701 tgatggggca gaaagtcgtt ttaagcgacc gctgctgcgg cgaatccggt atgttcgccg
     8761 tcaaacgccc cgacatcgcc acacaggtca agttccgcaa acaagaggaa atcgagaaaa
     8821 acctcaaaga gctgccgcag ggcgaacccg tcaaaatgct gacctcctgc cccgcctgcc
     8881 tgcaaggact gagccgctat tccgacgaca acaatatgcc tgccgactac atcgtcattg
     8941 aaatggcgaa acacatcctc ggcgaaaact ggctggatga gtttgtaaaa aaagccaaca
     9001 acggcggcgt agagaaagtg ttgctataac aacggacacg gaaatgccgt ctgaacgccg
     9061 aaagccttca gacggcattg tttgaatcaa atacctaccc gaatcatgat tgacggacaa
     9121 acaaccgaac ccaaacaaaa aaccagaatc ataccagccc ccatgcgggg gctggtagac
     9181 gatgtgatgc gcgacctgct gacacgtatc ggcggctacg acgaatgcgt cagcgaattt
     9241 gtacgcatta cccataccgt gcattcacga tccatatggt taaaatatgt ccccgaaatc
     9301 gccaacggaa acaaaacgtt ttccggcacg ccctgcaccg tccaacttct gggcagcgat
     9361 gcggacaata tggcggcaaa cgcattggaa gccgtccgat tcggcgcgga caaaatcgat
     9421 ttgaacttcg gctgccccgc gcccaccgtc aacaaacaca aaggcggcgc aattcttctg
     9481 aaagagccgg aactgatatt ccacatcgtc aaaacgctgc gcggacgttt gcctgcacat
     9541 attccgctga cggggaaatg cggctaggct atgaagacaa aagccctgct ttggaatgcg
     9601 cctgtgcgat tgccgaaggg ggcgcgtgcg gactgaccgt gcacgcgcgc accaaagccg
     9661 agggctacga acccccggcg cattgggaat ggataaggaa aatccacgat accgtcaata
     9721 ttcccgttac cgccaacggc gacgttttca gcctgcagga ctatatcggc atcaaaacaa
     9781 tcagcggctg caacagcgtg atgctcggtc gcggtgcggt catccgcccc gatttggcgc
     9841 gccaaatcaa gcaatacgag aacggcggac cggtcaaaga cacggatttt gccgaagttt
     9901 ccacatggat agggcagttt ttcgagctgt gcctgacaaa agaggcaaac aacaaatacc
     9961 cgatcgcccg tcttaagcaa tggctgggca tgatgaaaaa aacgttcgac ccggcgcaaa
    10021 cgctgttcga ccgcgtccga acggttaaag atgcggatga agtgcgccga atcctgaacg
    10081 catttgagca tgagatggat gtttgatgtc tcccgcgctt caaagcaaat gccgtctgaa
    10141 aacctttcag acggcatttg ttatctttat tgccgttttt cccctgcttt cggtgtattt
    10201 ttgtttttca gtttcgcccc caaacccaaa cgtttttcat aatccgattg cggggtatcg
    10261 tcttcctgca taccgaacgc gccggcattg acccacagcg acagcagcgc gacgacaaag
    10321 gcgcaaaagc cgattacata ccaaaacatt gcccctccaa atttgttaaa atcatatcaa
    10381 atacagtgcc gaatttatca caagcgcacg agcaaataaa tcttctgacc gcagtatttc
    10441 attcatttac cttgaaaaca aaaaagggca cctgaaaatt ttgcgaaact tttcagatgc
    10501 cctgctttgc cgaaaaacgt atcagccgca gcaaccgcct tcgccgtgtc cgcctttttt
    10561 gtcgttatcg acaatgggca gttcgacttt ttcgtttggt ttttctgcat tttcgggccg
    10621 gtcgttgagt ttgattttcg cggtttcgct catttcctta cctcatttgg aaaacggttt
    10681 gaaagattca taaatatagc atgtttaatc cgaatctgaa aagggaagcc gtatgcgaaa
    10741 tcccgtctga agcccaatca gggcttcaga cgggatttgc tatctttatt gccgcttttc
    10801 ttccgtatcc ggatttttgt ttggggctga agcagattgg cagtcagatt gcaatcgaag
    10861 aatgaaggca agccatcaaa aacaaagcta tccgcttcac cgccccgata tttagaattt
    10921 gtggcgcaga ccaacggagg cggcattaat ttgagtgtag ttgccgatgc cggtatttcg
    10981 tttcagccaa gcggcagaca tgatggcgga agtgcgcttg gaaaaatcgt aatcgacacc
    11041 ggcgatgatt tgatcatagc tggtatgttc gcgtttctga ctgcgttcga caaagtcgaa
    11101 accatgggca tagctgatgc gcgggactgt attaccgaag cggtaggaag cagtggcggc
    11161 aatttcggtc gtactgtttt tggttttgtc ggcattttca gacaaatcca actgagccgc
    11221 caaggcgaga ttcaagccgc cttccccata gccgcccgtc aggcggtgta cctgatggtt
    11281 tttcaatgga tcggtatcac tcgctctgcc gagcaagaac aaaaagaaag catcatgccc
    11341 ctcattggcg tgtttcgcat atttaagggc ataatttccg aaaaagccgc catttttgta
    11401 attcagaccg gcataataca catccgaccc cggcttgccg acaacagccg gaactggttt
    11461 catctgatta ctttccagcg tgaaagtagc aggcgtatag gcggacttgc tgttttgact
    11521 cggaacaaat tgaatgctgc cgctgaaacc ggaaaatccg ggggaatcgt aacgcacgga
    11581 aaccggcata ccgtcgtggc gtttgaaaat acccaattgc gaagccacat tattattgct
    11641 gtcccaagga tcaatggctt tgctggcatc gccaaacgga ttcgcaacgc gaccggcgag
    11701 cgccgtgccg aattcgcctg ccaagccgat aaaggattcc ctgttacccc aacgggtcgc
    11761 gccgccgccg gcaacggata cgtcttgctc gagctgccaa acagccttca gcccgccgcc
    11821 caaatcccca cccccttaaa gccgataaac gagccgaaat cactgacttt cgtcctgatg
    11881 cggcttttgg ccttagtaac tgtattgccc gtttgacctt ctgagggtgg ctcggtcaac
    11941 tgcagccgga tgttcctgcc ttccacacca gctttgactt cgccgtacag gccgacatcg
    12001 gcaactgccg caaacggcag tgcggacaat acgagggcgg taagtttttt tcgcatatcg
    12061 gcttcctttt gtaaatttgc taaaaacatc gggcaaacac ccaatcagtc tccaattacc
    12121 ccccgaaaaa aaaccatttt tcggaacaaa tttcgggtaa attttggaat ttttattttg
    12181 aaaaataata tttcaatata aaacaataaa ttaacttttt tgaaaatgtt gctttcccgc
    12241 aacatgattt aaatacagac cttgtcaaag ccgatgcggc gcagacccgg atgccgtctc
    12301 gttttttaaa acaccataaa aaaacacacg cggtacttta atttcctgcg tgtgtcgagt
    12361 gtatcgaaat caagccgaat caaatatacc ggacttcgat aatatcgtat tcgcgtacgc
    12421 cgcccggtgc ctgaacttcc gccgtatccc cttcttcctt gccgatcagg gcgcgggcaa
    12481 tcggagagcc gacatagatt ttgccctgtt tgatgtcggc ttcgtcttcg ccgacaattt
    12541 gataggtaac gtgttcttcc gtttccaaat cttccagcgt aaccgtcgta ccgaacacga
    12601 ttttgccttc ggcgtggatt tcggccggat tgatgatgtg ggcaacggaa agtttgtgtt
    12661 ccaactcgga aatgcggccc tcgataaaac cttggcgttc tttggcggct tcgtattcgg
    12721 cgttttcgga caaatcgccg tgcgatctgg cttcggcaat cgcttcgatc acttcgggac
    12781 gcgccacgct tttgagctgc tgcaattcct gtttcagcaa ttccgcaccg cgtacggtca
    12841 gggggatttt ttgcatcggt ctttttctcc atattccggc acaccggttt gcggcggcaa
    12901 gcataccgcg taccgtcttg ttttgtgcgt ccggatatta aaataaaaat acaagccgcc
    12961 cggaaaatcg gcggcttgtc tgtcgttgaa cagcggctat tctaccaaat tctatgaaat
    13021 tggcaatcgt gccgtgccgc cggcagacgc gctatgtccg caacaaaagc cgaaaatatg
    13081 ccggcaaaaa aattttagaa acaaaaaatt taaaaaaaat caattttcgg cataaaaaac
    13141 caaattcacg tactttaaaa ccgaaaatgc caaacctgag attttccaga cggcatttgc
    13201 accagtataa tgcaggctgt ttttatcttt atatttacgt tttgccatga ccgaatccgt
    13261 ccgcctcccc gccgcctcgc tcaaaccttc caccgtcgcc ctgcccggct ccaaaagcat
    13321 cagcaaccgc accctgctgc tcgccgccct gtccgacaat gtttgcgaaa tccattccct
    13381 gctcaaatcc gacgataccg accgtatgct cgaagcactc gataaactcg gcgttcaaat
    13441 cgaacatctt gccgaaggcc gtctgaaagt acacggcacg ggcggacgct tccccaaccg
    13501 ctctgccgat ttgtttttgg gcaacgcggg cacggcgttc cgtccgctga ccgccgcgct
    13561 ggccgttttg ggcggcgatt atcatctgca cggcgtgcct cgtatgcacg aacgccccat
    13621 cggcgatttg gtcgatgcgt tgcggattgc cggcgccgat gtcgaatatc tcggcaacga
    13681 acactatccg ccgcttcata tcggcaaacg tcaagactgc ggcgagcgcg tgattcctat
    13741 taaaggcaat gtgtccagcc agtttctgac cgccctcttg atggcgctgc cgctgaccgg
    13801 gcaggcgttt gaaatccgta tggtcggcga gttgatttcc aagccctata tcgacattac
    13861 tttgaaactg atggcgcaat tcggcgtaca ggttgccaat gaaggctacc gcgttttcaa
    13921 aatccctgcc gatgcgcact atcacgcacc cgaacacttg cacgtcgaag gcgatgcctc
    13981 cggcgcgtcc tacttcctcg cagccggttt gattgccgcc acgcccgtcc gcgttaccgg
    14041 catcggcgca aacagcatac agggcgatgt cgcctttgcc cgcgaactgg aaaaaatcgg
    14101 tgcggacgtg gtttggggcg aaaacttcgt cgaagtctcg cgcccgaaag gacgcgccgt
    14161 ccaagccttt gatttggatg cgaaccatat ccccgatgcc gcgatgaccc tcgccatcgt
    14221 cgcgcttgcc acaaggcaaa cctgcacgct tcgcaacatc ggttcgtggc gcgttaagga
    14281 aaccgaccgc atcgccgcaa tggcgaacga gttgcgcaaa ctcggtgcaa aagtcgtcga
    14341 agaagccgaa gcgattcaca ttaccccgcc cgaaacgccg acacccgatg ccgtcatcga
    14401 cacttacgac gaccaccgca tggcgatgtg tttctcgctg atttcgctgt tgggagtacc
    14461 cgtcgtcatc aacgacccga aatgcaccca taaaaccttc ccgacttatt ttgaagtgtt
    14521 ctcatcactg accgaaacag cggaataagg cggaaaaatg ccgtctgaaa cccgaaaaca
    14581 gggcttcaga cggcattttg ccgcgattcc ggcgcggcgg gcggctcatt ctgtaaaaaa
    14641 cgtatgcgct ccaaggtagt ttttggcgta aaacggtgtg gagagttttt cggttttgat
    14701 ggttttgccg ctgccggggg catggatgaa ttcgccgttg ccgatgtaga gtccgacgtg
    14761 tgagtagcgg tgtgcgccgc cggtgttgaa gaatacgagg tcgccggcct tgaggcggct
    14821 gtcggggatt ttgcggcttg ccgccgccat gtcgcgggcg gtgcgcggca gcttgacgtt
    14881 gagggcgttt ttgtaaacga attgaatcat gccgctgcag tcgaagccgg ttgcggtgct
    14941 gctgccgccc catttgtagg gcgtgccgat gagtccgagg ctgtggagca tgagttcctg
    15001 cgagccttgt gtgcggccga tgtggctgat gcggacggct tggatttgcc ggactgtctg
    15061 tttgggtttc ggttggcggt gtttgccgga ggtcgtgccg catgaggcga ggagcagtgc
    15121 gccgagacag aggaaaaggg ttttgtcggg ggaaaacatg gttttttcct ttgcgggttc
    15181 ggatatgccg tctgaaggtg tttcagacgg catttctata ataaaccttc gatgggcagc
    15241 agggatagga tttttgcggc gatgcgtttc caaagtttgg cttcgggttc gttcgggtag
    15301 gtttttcggg tggcgggatc gtgccattgc aggcggttgt gtttgtcgag ggtaacgcgg
    15361 taggcgtatt cgggtgtggt atcggcgagg gtgcgctcca tctgttctgc gattttgggg
    15421 ctttcgatga cgacgcccat ttcggtattg agccgtgcgg aacgggggtc gaggttgaat
    15481 gagccgatga agatgcgttt gccgtccaca atgaaggttt tggcatgcag gctggttacg
    15541 gagctgccgg tcaggccttt gtcttttgtg gcggggacgg catggttggg ttgcagctcg
    15601 tagagtttga tgccggcttt gagcagcggt tttcggtatt tgacgtagcc ggaatggacg
    15661 gcggcaacgt cggtcgcctg tagcgagttg gtcaggacgg taacgtctat gccgtcctgc
    15721 accagttttg ccagtgcgtc tgtgccggat tttgtaggga cgaaataggg tgaaaccaga
    15781 tagacgcttt tttcgggctg tttgagcgcg tcttgcagcc tcccggcaat cggcggtttg
    15841 cggcggtcgc ggtcgagtcc ttttgcaggg ttgtcgctga tcaggcgggt ttggacgctc
    15901 tgccagtcga tgcgtcccgt ctgtattttt tggtagaggg gcgactgttc gacggtttcg
    15961 cggtagcgca ggagcgcgtg tctggatgtt tcgtcgttgt atccgagtgc ttgaagaccc
    16021 ttgccgatgt tgccgctgcg gatgatgcgc gtggcgttgt gggcggaatg gcttgcccag
    16081 tagcggtcga agtcgtgcga tacttcgccg acgacgctgc cggtggcgag gatgtccagg
    16141 tcggcgaaaa cggtgtcctc accgactttg aagtattcgt cgccgatatt gcgtccgccg
    16201 agtatggtgg cgcggttgtc ggcggtaaag gatttgttgt gcatgcggcg gttgaggcgg
    16261 gggaagtcgg tcaggtagcc gagtgcgcgc catttgcgta ggacgaaggg gttgaacagg
    16321 cgcacttcga tattgggatg gctgtcgagg gcgagcagga gatcgtccaa cccgcgcgtg
    16381 ttgttgtcgt ccaacagcag gcgtacgcgc acgccgcgtt ctgcggcaag gtacatgagg
    16441 ttgaacagca gcctgccgga aatgtcgttg cgccaaatgt agtattgcaa atcgaggctg
    16501 tgttcggcag attcgataag ggcggcgcgg gcggcaaagg cttcgtgggg gtcgtcgagc
    16561 aggtagatgt cggatagccc gttgttatga ggggtgtgcc ggatttgcag gatgttgtcc
    16621 aggaggacag gtttggaagt attgaaatga cggctttccg tccgttcttc cagtgggggc
    16681 aaccatgaag aacatgaaca gagaaggagg cataaaaggg aaatgaggct gcgtgttttc
    16741 atcggggata tagtttcaga cggcattgcc tgtattttgg ggtctgtatg cattgaaatg
    16801 aagcatcata atctaaaggt tggaatgggt aaaagttttg tcgggatgcg gggttaaggc
    16861 cgcgtgcctc ttgtcaggtt tgcaccgtct gaaacgtgca gacgcttaaa aaccaacgac
    16921 gaggcaaggg tgatgccgcc gatactgagc agggtcatac ggaaggcgaa atgcaggcct
    16981 gaagaagccg gtatcagaaa tgtccagttt ttaaggatta atgcgccggc aacaatgccc
    17041 acgctgatgg caagctgttg gttgaccgcc atcaggctgt tgccgctgcc ggtctgttgc
    17101 gggcgcaaat cggcgagggt cagtgtgttc atggcagaaa actgtaggga gttgcatgcg
    17161 ccgatcgcca gcgagaggaa aatccaaatc cacagcggcg agtttccgtc aggcagggcg
    17221 agcagcatga tgaaggcggc aagcagcttg gtgttccaaa gcagtaccgt gcggtagccg
    17281 aaacgtttca tgagcggtgc aatcagcggt ttgaccagca gcgaagacag ggcgacgggt
    17341 gcgaccagcc agcccgacag gcttgcgccg aagccgaaag cgacttgaaa catcaggggc
    17401 atcagaaaag gaatcgagct gatgccgaga cggctgaaca gattgcccgc cagtcccaaa
    17461 cggaaggtgc gtatcagaaa caggtcggcg gaataaatcg gtttggacgc ggttttcata
    17521 tgtcggaaat aacggcgtgc aaacagcagt ccgccgcaca gcggtaacag tgcaaaatac
    17581 ggaggcagcg cgtgcgacag gctttctgcc gcaagcagca gcaggcaggc ggcaacggaa
    17641 aaggtcagat agcctttgaa atccagagcg gtatcgttgc ctttgacatc gggcatgatg
    17701 ttgcgtccca atatgaaacc cagcagaccg atgggcaggt tgagcaggaa aatccagtgc
    17761 cacgaagcgt attcgaccag ataaccgccc gccagaggtc ccaacgcagg gccgatcagt
    17821 gcaggcatga ccgcataatt gatggcgttg agcagcttcg atttttcata tacacgcaag
    17881 atggtcagac gcggtatcgg aaccagcatc gaaccgccga tcccctgaac gacacgggaa
    17941 agcgtcaatt caaacagcga acccgatgcg gcgcacaatg ccgatccgag cataaaaacg
    18001 gcaatcgaac cgaaaaagac ttttttcgtt ccgaacttgt ccgccaaata accgctcaaa
    18061 ggaatcagca gggcaaccgt cagcgtatag gcaacgactg cctgttgcat atccggaggc
    18121 gattcgttca ggtcggcggc aatttcgggc agtgcggtat ttaaaatggt cgcatccaac
    18181 atctgcataa aaatggcaat tgccagcaga agcggcagcc aaggggatgg tgcgcgggcg
    18241 gacagggtgt ttttttccat agggtgattg taccccatcc ttgtgccgtt attgttttca
    18301 gatgctgtct gaatgccggc agagtcggca tcctgaatgt tcacaagcaa acgaatcggc
    18361 attgcattgt aatgataatt attatcgaaa atcatcagag taaggtacag taagcgttat
    18421 ggggcagttt gtaagaaaaa ccggattatt ttttaaaatt agacttgacc cgcaacggtc
    18481 aattacttaa agtaaacgct tacctttcta cagaaaaaac gggtttcccg ttatcaaaaa
    18541 atatgagcgc aaccattccc ccaaaaatca tccgatacga cagcaatccg acagatgtct
    18601 attttttcgg cacttgcgtc cttgatcttt ttatgcccga agcaggcatg gatgccattg
    18661 ccctaatcga gcagcagggc atacgcgtcc atttcccgat ggcgcaaagc tgctgcggtc
    18721 agcctgccta ttcatccggc catccgaccg aagccttcga tgtcgccaaa gcgcaactcg
    18781 accttttccc cgaaaactgg ccgattgtcg taccgtccgg ctcatgcggc ggcatgatga
    18841 aacatcactg gcctaccctg tttaaaaata cagaatacga atccaaagcg gttgattgtg
    18901 ccaaccgtat cattgagttt actcatttct tgcttgccat cggctacaag cctgaagaca
    18961 aaggcgagcc agtcaaagtc gccgttcaca cttcctgtgc cgcccgccga gaaatgaatg
    19021 tccatctttc aggctggcag ttgattgacg gtatggaaaa cgtcgaacgc atcgtccacg
    19081 accacgaaag cgagtgttgc ggcttcggcg gcacattctc cgtcaaacaa gccgatattt
    19141 ccggcgcgat ggtaaccgac aaagttgccg cgctgaaaga aaccggcgca accgaaatca
    19201 tcagcgcgga ctgcggctgt atgatgaaca tcggaggcaa aatcgccaag gacgagccgg
    19261 atatgccgcg tccgaagcat atcgcatcct tcttgttgga acgcaccgga ggcaaagtat
    19321 gagcgcgcgc gaaaatattt tggcaaaact gaaaaaagcc ggcgcattgc cgatggaaga
    19381 gcctgcggtt tttgattatt accgcgaaaa gggcgtttct tgggacagcg aagccgagcg
    19441 tctgaaacat tgggctgccg ccatgcgcgc ggtcaaaacc gaaatctatt gggtgacgaa
    19501 aagcaattgg atgcaggttt tccgcgaagc ggcagaaggc aagggtttaa aaaacatcct
    19561 gctgcccttg gcgaccgaac acggacaaat tgcccgtgcc gcattggcgg gcagcaatat
    19621 cgacccgatt gccttcgagc gcgaaatcga tacttggaaa accgagtttt tcacgaacat
    19681 cgatgcaggc ttcagcggcg cgcaatgcgg catcgcccgc accggcacgc tgatgctgtt
    19741 ttccagccct gaagaaccgc gtactttgag cctcgttccg cccgtgcatt tctgcctgtt
    19801 cgatacgtcc aagatgtaca acgagtttca caacgccgtc gaaggcgaaa aactggtgga
    19861 aaacggtatg ccgaccaatg tattcctgat ttccggcccg tccaaaaccg cagacatcca
    19921 gttgacgctc gcttacggtg cgcacggtcc gcgtgatttg gtcatcctcg ccattttgcc
    19981 cgaccacatt tcccctgccg atttggagga aaacgcatga ctacgcaaac catcaagttc
    20041 cacatgaagc cggaaacttt caaacaaaac gccgcaattt cccttcaaga caagcccttg
    20101 cgtaaaagcc tgcgtaccgc gatggatatg ctgatgacca agcgcaaagc cgttttgacc
    20161 gacgaagaag agttgcaaag cctgcgcgat ttgtgcgaac acatccgtca gcgttccctc
    20221 tccaaattac ccgcgctgct ggagcagttg gaagaaaacc tgactaagtt gggcgtgaaa
    20281 gtacactggg cggaaacccc tgccgaagcc tgccaaatta tccacgacat catcacagcc
    20341 aaaaacggca agctgatggt caaaggcaaa tccatggtca gcgaagaaat cgagctgaac
    20401 cattatcttg aagcaaaagg cattaaggcg gtggaaagcg acttgggcga gtttatcgtc
    20461 caaatggcag gcgaaaaacc gacccatatc gtgatgcccg caatccataa aaccaaagaa
    20521 caggtcagcg aactgttcca ccaaaacctc ggcacaccgc ttacagacga cgtggaccaa
    20581 ctgaccggat tcgcccgcaa agccctgcgc gatatttaca gcactgccga tgtcggcttg
    20641 agcggcgtaa acttcgccgt tgccgaaacg ggcacgctgt gtctggtcga aaacgaaggc
    20701 aacggccgct tgagtactac cgttccgccc gtacatatcg tcattacggg tattgaaaaa
    20761 gttgtcgcca agctgtctga tgttccgcct ttgtacagtc tgctgccgcg ttccgccatc
    20821 ggtcagaaca ttaccactta tttcaatatg attaccggcc cgcgccgcag tgaagaatta
    20881 gacggtccgc aggaaatgca cttggttttg ctcgacaacg gccgcagcca agcttatgcc
    20941 gaagaccaaa tgcgccgcac cctgcaatgt atccgttgcg gcgcgtgtat gaaccattgc
    21001 ccggtttata cacgcatcgg cggcgcggca tacggcacaa cctatcccgg tccgattggc
    21061 gagattattt ccccgcacct gttgggtctt gatgccaccc gcgacctgcc gactgcctgc
    21121 acgatgtgcg gcgcgtgcgt ggaagtttgt ccggtacgca tcccgattac cgaacaaatg
    21181 caacgtttgc gcgttgaagc acaacgttcg ccgaccgaaa ccgtgccgca ccccatccga
    21241 gggcaaggcg catcgcatac cttcggcgaa caaatggcgt ggcgcacatt caacggtatt
    21301 ttcagcggta gcaaaaccta ccgcgccttc ggttgggcag ccaccaagtt ccgcaatctg
    21361 accccgcgca aacagttggg ttggacgcaa aaccgcgtac cgatgaaacc ggcgaagaaa
    21421 accctgcacg aactgatggc ggaaaaaatg cgccaaaaag aacaggcata aaaagttgtt
    21481 cgcaaaaata ccgtctgaaa cccgaaaaca gggcttcaga cggcctgaaa agcagcctga
    21541 acgcgcaaaa ataaataatg aaacggaatg ctaagacgct ttcaggcagc ctgacattaa
    21601 ggctgcttaa aagcgtcttt taatcgtgcc acggattgaa caccgcgaca tcggccgcaa
    21661 aaaatgagcc ggtatcacgc gtagcaactg tcaaactgtg ctgttttgca gtggcggcaa
    21721 tatagccgtc tgcggcagca atctctttgc catgtgtttt ggcataggaa cgaatttgcg
    21781 catagattgc ggcaaccggt tcatcaaaag gcagaatccg ccccgcaaat aaaggcaaaa
    21841 tggattgttc caaacgttcg tgcagcacat tctttttctt gccattgagc aacaacgcca
    21901 cacccaaacg caattctgca acagtaatgg cagacaaata cacatcttcc aatatcaaac
    21961 tatccaacca tgccaccaca cgttcattgg gttgtgggcg caaaggttcg gaaatcacat
    22021 tcgtgtccag caaaatcatc acaaagaaac ctcgttatca gtattacgac cgcgtacgtc
    22081 ttccagctca acacctccga tttcctgccc tattgatgcc aacattgacc ccaaacgtac
    22141 agtttgttgt gctttggcga tgttatccaa aattaagcgg atttctgctt cggtactgcg
    22201 ccctgcggct cgcgcacgga atttgattgc gttgtgcgtg gcctcggata aatttctaat
    22261 cacaacagaa gccattgcct ttccttcctg aaaaaagttt ttcaaatgct atcaaaataa
    22321 aaaaatgata gcaatctatt ttttaacgcc tgcggatgcc tgaaaattca ggtggccgca
    22381 ggcgtttttt tacatctaaa aaaacgtctc ccctccctct ttctgataaa taaaaaacag
    22441 tcgctcttga actgcacccc aaaaagtcgg acagtttaat caagcggctt tcagggactg
    22501 aattctgtac tgaacagggc tcagtccttt taatttcaac ttgattctat cgttgttgta
    22561 gtaacggata tattcgtgca gtgccgcttc cgattcggta acggaatcat atttgcacgt
    22621 atggaaacat tccgatttca acgttccgaa gaaactttcc attgccgcat tgtccaagca
    22681 gtttcccttg cgggacagac tctgaaccag accgttgcct ttcaactgct tttgataaaa
    22741 aacatctgat attgccaacc ttgatccgaa tgcagcatcg gcttttcaga cggccccggt
    22801 ttctccggcg cacctttcag tatctcgccg gccaaatcga aagtcgggcg ggtttgaata
    22861 cggtaactga cgatttcccc gttaaacaaa tccataatcg gagaaaggta tatcttttct
    22921 ccgcctacat cgaactcggt aacgtccgtt acccatttct cattcggctt ttctgaatgg
    22981 aaacagcgtt gcaggatgtt ctgcgcaact ttgccgactt ctcctttgaa cgagcggtat
    23041 ttgcgccgcc gtatcactgc cttcagcccc gtcttcgcca tcagacggct gactttcttg
    23101 tgattgaccg gtattcctgc gtgacggatg gctgccgcaa tcctccggta gccgtatctt
    23161 cccttatatc gttgataaat atcatggata tgtcgtttca aatcggcata tttgccttct
    23221 gccgattgga cggccaattg gtaatagaag gtgctgcgtg gcagtccgac aatccccaac
    23281 agcagttcaa cggatggcat ttccttaacc ctgcgacaag ttgcgttctt tctgccgcac
    23341 ttctttcaag gcatcgagct tttttaggac agccgcttcc gctttaaggt aagccaattc
    23401 cgcaaacagt tcttccttgg ttttcagata gtcggctttt ttcgtttgcg gcggatactg
    23461 ttttttcacg ggctttcttc ctttgggttt gggctttaaa ccgttaatac cattcaaacg
    23521 gtagaggcgc aaccattgcg gcaaaacgga gcattcgggc agattcagtt ggtctgcggc
    23581 agctttttgg gacattccct gccccgtcac cgggcggatt gcctcaagtt tgtattcgac
    23641 cgaatatttt gtcgtatgct ttctgcgttt gatgccgctt tcaccgtgta atctgtatct
    23701 tgccacccat ttgcgtacca atgaatcgga aatggaaaaa tatagtgaat taacaaaaat
    23761 caggacaagg cggcggaccg caggcagtac aaatggtacg gaaccggttc gcccggtgct
    23821 tcat
//
LOCUS       DAXFZR010000026        23682 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000026 DAXFZR010000000
VERSION     DAXFZR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23682
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.026"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            198..422
                     /locus_tag="ACFJAS_000876"
     CDS             198..422
                     /locus_tag="ACFJAS_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982162.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQGSIGKTEEPSYFLMFMFL
                     NSLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            478..1134
                     /locus_tag="ACFJAS_000877"
     CDS             478..1134
                     /locus_tag="ACFJAS_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC3982163.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVLEKLGKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGVDGNTVLLADPSPGHVSM
                     SRAQFLEAWQTREGNLAGKILAVVPKKAEAISNKLFFTHHPKRQTEFAVGQIRQGRAE
                     "
     gene            1262..2557
                     /gene="hisS"
                     /locus_tag="ACFJAS_000878"
     CDS             1262..2557
                     /gene="hisS"
                     /locus_tag="ACFJAS_000878"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC3982164.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            2558..3187
                     /locus_tag="ACFJAS_000879"
     CDS             2558..3187
                     /locus_tag="ACFJAS_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC3982165.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALMILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            3337..4794
                     /gene="der"
                     /locus_tag="ACFJAS_000880"
     CDS             3337..4794
                     /gene="der"
                     /locus_tag="ACFJAS_000880"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC3982166.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            4825..5724
                     /locus_tag="ACFJAS_000881"
     CDS             4825..5724
                     /locus_tag="ACFJAS_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC3982167.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            5776..6193
                     /locus_tag="ACFJAS_000882"
                     /pseudo
     CDS             5776..6193
                     /locus_tag="ACFJAS_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6260..6826
                     /gene="dcd"
                     /locus_tag="ACFJAS_000883"
     CDS             6260..6826
                     /gene="dcd"
                     /locus_tag="ACFJAS_000883"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC3982168.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(6892..7596)
                     /locus_tag="ACFJAS_000884"
     CDS             complement(6892..7596)
                     /locus_tag="ACFJAS_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC3982169.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGTVQF"
     gene            7740..8935
                     /locus_tag="ACFJAS_000885"
                     /pseudo
     CDS             7740..8935
                     /locus_tag="ACFJAS_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            8961..9914
                     /locus_tag="ACFJAS_000886"
     CDS             8961..9914
                     /locus_tag="ACFJAS_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC3982170.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEITPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(10019..10342)
                     /locus_tag="ACFJAS_000887"
     CDS             complement(10019..10342)
                     /locus_tag="ACFJAS_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982171.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(10515..11873)
                     /locus_tag="ACFJAS_000888"
     CDS             complement(10515..11873)
                     /locus_tag="ACFJAS_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC3982172.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQEKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(12231..13931)
                     /gene="recJ"
                     /locus_tag="ACFJAS_000889"
     CDS             complement(12231..13931)
                     /gene="recJ"
                     /locus_tag="ACFJAS_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC3982173.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(14000..14713)
                     /locus_tag="ACFJAS_000890"
     CDS             complement(14000..14713)
                     /locus_tag="ACFJAS_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982174.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     TAVLVLPMALIAAANSSDK"
     gene            complement(14760..15266)
                     /gene="yjgA"
                     /locus_tag="ACFJAS_000891"
     CDS             complement(14760..15266)
                     /gene="yjgA"
                     /locus_tag="ACFJAS_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC3982175.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTRLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     QARVRLLADEGALTQFMSDFPNAGAGRLRTLVRNTKKEQEQDKPPKNFRALFQELKTV
                     MENGGAEI"
     gene            15420..16751
                     /gene="pmbA"
                     /locus_tag="ACFJAS_000892"
     CDS             15420..16751
                     /gene="pmbA"
                     /locus_tag="ACFJAS_000892"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC3982176.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRNIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(17050..17253)
                     /locus_tag="ACFJAS_000893"
     CDS             complement(17050..17253)
                     /locus_tag="ACFJAS_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC3982177.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            17498..17800
                     /gene="clpS"
                     /locus_tag="ACFJAS_000894"
     CDS             17498..17800
                     /gene="clpS"
                     /locus_tag="ACFJAS_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC3982178.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            17802..20093
                     /gene="clpA"
                     /locus_tag="ACFJAS_000895"
     CDS             17802..20093
                     /gene="clpA"
                     /locus_tag="ACFJAS_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC3982179.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINGRFLPDKAIDVMDEAGAAQRILPKSKRKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(20133..23231)
                     /locus_tag="ACFJAS_000896"
     CDS             complement(20133..23231)
                     /locus_tag="ACFJAS_000896"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC3982180.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSGNSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKHNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILNRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKGKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(23462..>23682)
                     /locus_tag="ACFJAS_000897"
     CDS             complement(23462..>23682)
                     /locus_tag="ACFJAS_000897"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3982181.1"
                     /translation="KGAKMPRGSKAAIMQYKIPIPPLPEQEKIVAILGKFDTLTHSVS
                     EGLPHEIALRRKQYEYYREQLLAFPKAA"
BASE COUNT         5691 a         6655 c         5924 g         5412 t
ORIGIN      
        1 agccgcagac agtacaaata gtacggaacc gattcactcg gtgcttcagc accttagaga
       61 atcgttctct ttgagctaag gcgaggtaat gccgtaccgg tttaaattta atccactata
      121 tatttatccg atatatcaac atctctctat cttattccga attttattgt tgaaacttgc
      181 atgaaaaacc atacaagatg atgaagaaca aatatatttt aataaaagca gcaatcggca
      241 taaccgccat atccatattt tatcttctga ctcaaggaag catcggcaaa actgaagaac
      301 catcatattt cttgatgttc atgtttctca actcgctttg gtttgaagaa aataaaacag
      361 ttatggctgc cgttacggca atgattgccg cccactttat atttgtcgca ctatccgatt
      421 aataagcaaa caaaaaggct gcttggaaat ttcaggcagc ctttcaggag gattttgatg
      481 gaacaaaaaa ggcggtttgc cgcttccctg ctcctcgccg ccgccctgcc gctttgtgca
      541 cattcttttc cctttgcgga agaaaacccc atagcctacg gcaaagtcaa aatacaaagc
      601 tggaaagcgc ggcgggattt caatattgta aagcaggatt tggatttttc ctgcggggcg
      661 gcttcggtgg cgacgctttt gaacaatttt tacgggcaaa agctgacgga agaagaagtg
      721 ttggaaaaac tgggtaagga acagatgcgc gcgtcgtttg aggatatgcg gcgcattatg
      781 cctgatttgg gttttgaggc gaagggctat gccctgtctt tcgagcagct cgcgcagttg
      841 aaaatccccg tcatcgtgta tctgaaatac cgcaaagacg accatttttc ggtattgcgc
      901 ggagtggatg gcaatacggt tttgcttgcc gacccgtcgc cgggtcatgt ttcgatgagc
      961 agggcgcagt ttttggaggc ttggcaaacc cgtgagggaa atttggcagg caagattttg
     1021 gcggtcgtgc cgaaaaaagc ggaggcgatt tcaaataaat tgtttttcac acatcatccc
     1081 aagcggcaga cggagtttgc agtcggacaa atcaggcaag ggcgcgcaga gtaagtcaaa
     1141 tgccgtctga aaactgtttc agacggcatt tttacccgtc cgcaaaccat ctccatcctc
     1201 acaagccctt aaaaatccgc tataatcgcc cgcaatttga tttcagcacc ccctttttga
     1261 aatggcacaa aaaatccaat ccgtcaaagg catgaacgac cttctgcctg tcgggcaaaa
     1321 agatttcaaa ctcacggctg tgttttggca ggcgtttgag gatacggtaa accgttggac
     1381 gcgagcttac ggttatcagc aaatccgtac gccgattgtc gagcaaaccg gtttgtttgt
     1441 ccgctccatc ggcgaggaaa ccgatgtagt cggcaaggaa atgtacacct tctccgattc
     1501 aaacgattct ttgagtttga gcctgcgtcc cgaaggtacg gcctcttgtc tgcgtgcggt
     1561 ggtcgaacac aaccttctgt acaacagccc gcaaaagctg tggtatatgg ggccgatgtt
     1621 ccgccgcgag cgtccgcaaa aaggacgcta ccgccaattt catcaggtcg gtatcgaggc
     1681 tttgggtttt gaagggccgg atattgatgc ggaaatcatc gcaatgtctg ccgacttgtg
     1741 ggaaaaattg ggtattcgcg aatacctgac tttggaaatc aacagcttgg gcaaccgtga
     1801 ggaacgcgcg gcacaccgtg cggcattggt tgaatatctg acccgctatg aagcgcaact
     1861 ggacgaagac agcaaacgcc gtctgaaaac caatcctttg cgcgttttgg atacgaaaaa
     1921 ccctgatttg caggaaatct gcaatgccgc gccgcgtttg gtagattact tgggcgaggc
     1981 ttcgcaaaac cactatgcac gcttcaaggc gatgttggac ggtttgggta ttcaatatat
     2041 tgaaaattcg cgcttggttc gcggtttgga ttattacaat cagacggttt ttgagtggac
     2101 gaccgacaaa ctcggcgcac aggcgactgt gtgcggcggc ggacgttacg acggtttgat
     2161 tgaagaactc ggcggcaagc ctgcgccgtc catcggcttt gcgatgggca tcgaacggct
     2221 gctgcttttg gtgagcgaat acggttcgct ggaagtgaac gccgcgcctg atgtctatgc
     2281 aatgcaccaa ggcgaagggg cggacttgca ggtgatgaaa tacgcacaag ccttgcgcgc
     2341 gcaaggtttc aatgtaatgc agcattccgg ctatcaaagc ctgaaggcgc aaatgaaaaa
     2401 agccgacaac agcggcgcgc gctttgccct gattgtcgcg caagacgaac tggcggacgg
     2461 taccgttacg ctcaaagaca tgaacggcgc acacggtcag caaaccgttt ccgccaccga
     2521 cttgacagat actttacaac aatggaagaa cgcataaatg gcagcccatc tcgaagaaca
     2581 acaagagtta gacaacttta aatatttttg gaaaaccacg ggcaaatggc tgtttgccct
     2641 gatgattttg gcggcactcg gctacttggg atacacggtt taccaaaacc gtgcggcttc
     2701 ccaaaatcag gaagcggcgg cggtgctggc aaacatcgtg gaaaaggcgc aaaacaaagc
     2761 cccgcaaagc gaaatcaatg ccgaactgtc caaactccaa caaagctacc cccattccat
     2821 ttccgccgcc caagccacgc tgatggcggc ggcaaccgaa tttgacgcgc agcgttacga
     2881 tgttgccgaa ggtcatttga aatgggtgtt gtccaaccaa aaagacagcc tgattcaggc
     2941 gttggcggcg cagcgtctgg gcgttgtgtt gttgcaacaa aaaaaatacg atgccgcgct
     3001 tgccgcactc gacacgccgg ttgaggcgga cttcgccccc ctgctgatgg aaactaaagg
     3061 cgatgtttat gccgcacagg aaaaaagcca ggaagcctta aaaaactacg gacaggcttt
     3121 ggaaaaaatg cctcaagatt ctgtcggtcg cgaattgctt caaatgaaac tcgattcgct
     3181 gaaataaata ccgtctgaaa tgccgctctc tcttcagacg gcatttcaga caaccccatc
     3241 ctacggcaat tcccgtaaaa ccatccgccg tgtcccaacg gacacctgct cgtgttcagt
     3301 tctaccgaac ggcacttgac acagaaagac ccaatcatga aaccaaccat cgcgcttatc
     3361 ggccgcccca acgtcggcaa atctaccttg ttcaaccgtt tgacgcgcac caaagacgcg
     3421 ctcgtgcatg acctgccggg tctgacccgc gaccgccatt acggacacgg caaagtcggc
     3481 agcaaacctt attttgtcat cgataccggc ggtttcgagc cggttgtgga cagcggcatt
     3541 ttgcacgaaa tggcaaaaca aaccttgcag gctgtcgatg aagccgatgc ggttgtgttt
     3601 ttggtggacg gccgtaccgg tttaacaccg caagacaaga ttattgccga ccgtttgcgc
     3661 caaagtccgc gtcccgttta tttggccgtg aataaaggcg agggcggcga cagggccgta
     3721 cttgccgccg agttttacga gctggcattg ggcgagccgc acgttatttc cggcgcgcac
     3781 ggcgacggcg tgtattacct gattgaagaa attttagaaa acttccccga gcctgaagcc
     3841 gaagaagccg atgcaaaaca tcccgttttt gccgttatcg gtcgtccgaa cgtcggcaaa
     3901 tctacgctgg ttaacgccat tctcggcgaa aaacgcgtca tcgccttcga tatggcaggc
     3961 acaacgcgcg acagcatcca catcgatttc gagcgcgaag gcaaaccgtt taccatcatc
     4021 gataccgcag gcgtgcgccg tcgcggcaaa gtggatgaag cggtggaaaa gttctccgtt
     4081 atcaaagcca tgcaggcggt tgaagcggca aacgttgctg ttttggtatt ggacgcgcaa
     4141 caggacattg ccgaccaaga tgcgacgatt gccggttttg ctttggaagc gggacgcgcg
     4201 ctggtagttg ccgtcaacaa atgggacggc atcagcgaag agcggcgcga acaagtgaaa
     4261 cgcgacattt cccgcaaact gtatttcctc gattttgcca agttccactt tatttccgcg
     4321 ttgaaagagc gcggtataga cggtttgttt gaaagcattc aagctgccta caacgcggcg
     4381 atgattaaga tgccgacgcc gaaaatcacg cgcgtattgc aaaccgccgt cggacgccaa
     4441 cagccgccgc gtgccggctt ggtgcgtccg aaaatgcgtt atgcccacca aggcggcatg
     4501 aatccgccgg tgattgtggt tcacggcaat tcgctgcacg cgatttccga cagctatacg
     4561 cgctatctga cccagacctt ccgcaaagcc ttcaacctgc aaggcacgcc gttgcggatt
     4621 caatacaatg tttcggaaaa cccgtatgaa aatgcggaag acaaaccgaa gaaaaaaccg
     4681 ctgcgccgcg tcagcctgag caaccgtatt gagaaacgcg aaggccgaaa ggaagagaaa
     4741 aaccgcttca agaagaaaac caaagtcagt gtgaaaaaac aattcagcaa ataattcccg
     4801 acatttcaaa caataggaaa cattatgtgg ttcaagcaga ttagttttta tccgctcaac
     4861 aaagaaaagc tgcctgaggc ggacgtactt gccgacaaac ttgctgaagc tgaatttacc
     4921 cattgccaag gtttggactg gttcagcgaa ggctttaccg caccggtttc tttttcgccc
     4981 gaactcgttt tccctgccga ctttaccttg cgcgtcgccc tgaaaaaaga ggaaaaagtc
     5041 ctgcctgccg gcgtcatccg cgatattttg gaagagaagg tagcggaaat ccaaaacaat
     5101 gaagcccgca atgtcggtcg caaagaaaaa caagagctta aagagcaaat tacagacgac
     5161 ctgctgcccc gagcgtttac ccgcagcagc cgtacagaag cggtgtttaa cacccgccac
     5221 ggctacctgc tcgtcaataa cgcggcttcc gccaaagcgg aaaacatcct gaccaagctg
     5281 cgcgaagctt tgggcggttt ggaagcctcg ctgccgaata ccaaacaatc gccctcttcc
     5341 ctgatgaccg gctggctgtt gcaagggcat tgcgaaggcg gttttgaatt agacagcgat
     5401 tgcgaactca aaggtacggg cgatattgtt cccgtcgtca aagtatccaa acaagactta
     5461 accgccgacg aagtggttca acacgtcaaa aacggtaaaa ccgttacccg gctcggtttg
     5521 gtttggcgcg aacaaatcgc ctttatcctc actcaagact tcacactcaa gcgcatccaa
     5581 tacctcgacg tattgcagga agaagccgaa agcaacggcg acgatgccgc cggccttgcc
     5641 ttcgcctcgc aaatcctgat ggcggaatcc gtcagcacca tgttggaaga gctggtttcc
     5701 tatttgggcg gctggcaaga ttgatttttc agacggcttt tataccgtca gccaaaatcc
     5761 aatggaataa cccttatgct taaacatctc gcattcctac tgcccgccat gatgttcgcc
     5821 ctccccgccc agaccgccgt cctaagcccc tatcaggaaa ccggctgcac ctacgaaggc
     5881 gggatcggaa aagacgggct tccttcaggc aaaggcatat ggcgttgccg ggatgggcgc
     5941 ggttataccg gttcattcaa aaacggcaaa ttcgacgggc aaggcgttta taccgttgcc
     6001 gccggccgcg aagtatttct cgagccgttc aattccgaca gtaccaaatt ccgcaatatg
     6061 gcattgtcgg gcacgttcaa acaaggcttg gcacacggca ggttcgccgc ctcgcaaaac
     6121 ggcgaaaccc tcttttatta tgaaatgcga acacggcatg attaaagaag tgaaactgcc
     6181 caaaaacaaa taaccccccg tttttcaaac agcatccccc aatgctttat ccgaacaccg
     6241 caaaaaacaa ggaaacccca tgagcatcaa gtccgacaaa tggatacgcc gaatgagcga
     6301 agaattcggc atgattgatc ccttcgagcc gaaccaaatc aaagaagccg acggacagcg
     6361 catcatctcc tacggtacgt ccagctacgg ctacgatatc cgctgcgcca atgaatttaa
     6421 aatttttacc aacatcaaca gcaccatcgt cgatcccaaa aacttcgacc cgaaaaattt
     6481 cgttaccgtc gaagacgact gctgcatcat cccgcccaat tccttcgcac tggcgcgcac
     6541 ggtcgaatat ttccgcattc cgcgcaacgt cctgaccgtc tgtttgggca aatccaccta
     6601 cgcccgctgc ggcatcatcg tcaacgttac cccgttcgag ccggaatggg aaggctacgt
     6661 taccctcgag ttttccaaca ccacccccct gcccgccaaa atctacgcag gcgaaggcgt
     6721 ggcgcaagtc ctcttcttcg agagcgacga agtgtgtgaa acttcataca aagaccgcaa
     6781 cggcaaatac atgggacaaa ccggcgtaac cctgcccaaa gcctgaaata caaaatgccg
     6841 tctgaaccct tatcgtcggg tttcagacgg catctaccta tttatccgtg gttagaactg
     6901 aaccgtccca ttgatgctga tgctgatttc ctccacaccc ggtgcggctg aatccgtacc
     6961 cttcatattg acgcttgccg ccatcggcat cgcgcgcagc atttttgccc gaacagcccc
     7021 atcgcccgca atatggctgc cgatttgccc aaaattcaat ttgacgattt tataaccgga
     7081 cgcacccaga acgcccgcca gtttttcggc acgcgccttg aaacgcaaaa cggcatcctt
     7141 gctgacctga tcgatgacct cgtttcggcg ttcgcgcgac acgctgaaat cggtatcttc
     7201 aagcgaagca tccgtctgaa catcagcaat aaaacggttt aaagcatcaa aatccctgcc
     7261 ctccgcctta aattccgcac gctcctccca gcctgtttga atgcgtctgc cgttggtata
     7321 ttgatagcgc ggcatcgcac tgcgcgatac caattcggtt ttaaagctac catttttcga
     7381 ttttctggta aaattgttga attttttaac aaactcggca ttgacggcat ttttgtcccg
     7441 tccttccgcc gccacctgga aacgcgcgga cattgtatcc tgagccacct cgatacccgc
     7501 cgattcggaa aattcgacaa tattgtaatt caatgcctca gccgccgccg gaaaagatac
     7561 cgccagcagg gaagccgcca aaacagaacg cagcatatcc gtatccttac ttgaaaaacc
     7621 gccaatctaa catataggta cgctaaattg cgttagcata agacaacaat ttaaatattc
     7681 gatcggtctg ccttataata accgtctttc cgatactgaa tcatattatg tccgaaccgt
     7741 tgcacgtcct cgtcatcccc ttatggtatc cacgatccga acaggatgtg gacggaatat
     7801 ttttccaaaa tcaagcacag gccctccaac gcaaaggcat caaaaccgcc atcctcgccc
     7861 ccatgttccg ttatctgcgg aaagaaacag caagcatcct gaccggtcct tacggttttg
     7921 cccgataccg gcaaagcggt ttggacatct acgcatggcg cagcatgtat ttcttccccg
     7981 ctttccgttt atcgacatcg accgcatccg ctgggtgcgc gccggtttga aagccttcaa
     8041 acactacatc cgcgaaaacg gactgcccga cctgattcac gcccactgca tgaactatgc
     8101 aggcatactt gcccaaaaaa tttccgaaaa atacggcatt ccctacgttt taaccgaaca
     8161 cagcagcacc atcacgcgcg gtctgatccg tcaccaccaa tggcagccga tggaaaaagc
     8221 agcggcgccc gcctccgcac gtctcgccgt cagccgccat ttcgcacacg tcctgcaaca
     8281 caaatacggc tgcgaatggc agtacctccc caacataccg ggtggaatat tcaagcaaac
     8341 atttgaataa aaagaaaaaa ataacaaacc gcatttcgtg ttctgcaccg tctcgcacct
     8401 tcgccgcctc aaaggacacg atgtcctgct cactgctttt gcccgggcgt tggcacaatg
     8461 cccgcaactg cgcctgaaca tcggcggcag cggacaggaa gaacagcggc tgaaacagca
     8521 ggcggcagac ttgggcatcg cccatgccgt tacattttta ggcgcactgc aacccgaagc
     8581 aatcttggat ttgatgagga acagcgacgc cttcatcctc gccagccgca ccgaaacctt
     8641 cggcgtggtc tatatcgaag ccctctcgca agggttgccc gtcatcgcca tacattgcgg
     8701 cggcgcggaa tccatcgttt cagacggcaa cggatacctc gtttccgttg acgatgccga
     8761 tgtcctagcc tttctcaaaa tatatgaaca ccatgccgat ttttccgccg aacagctcag
     8821 aaccgactgc ctcgcaacat tcggcgaaga tgccgttatc ggcagactga ttgccgtatt
     8881 ccgtcaggcg gcgcggaaca cggcgcgaaa acgcccgaaa aatagattaa aatgacgcaa
     8941 aacaaccttc ggattcaaat atgacccata ccgtccacct gcacttggaa gaaaccgaca
     9001 acccgacgct gcaacgcctg tgcggttctt tcgacagcaa cctcgattcg ctcgccaaag
     9061 cactcgacat ccatatcagc cgccgtttcg agcattttac cttcaacggt gcatttgcac
     9121 acgccggcaa acgcgcactg ctcaaactct tggaaacggc gcagacgcgc gacctaaacg
     9181 acagcatcat ccgccttgcc gccgtcgaaa cccaaaccga agatgccggg catcaggaaa
     9241 aaaatcacga acacgcctat tatttccgaa ccaagcgcgg cagcataggc ggcagaacgc
     9301 cacggcagaa cggctatatc cgcgccctgc tcgaccacga catcgtattc ggtctcgggc
     9361 cggcaggtac gggcaaaacc tatctcgccg tcgccgccgc cgtcgatgcg atggaaaaac
     9421 accaagtcga acgcatcatt ttagtgcggc ctgccgtcga agccggtgag aaactgggct
     9481 tcctgcccgg agacctgacc caaaaagtcg atccctacct ccgtccgctt tatgatgccc
     9541 tctatgacct gatgggcttt gaccgtgtaa ccaagctgat tgaaaaaggc ctgattgaaa
     9601 tcaccccgct cgcctatatg cgcggcagga cgctcaacgg cgcatacatc atcctcgacg
     9661 aagcgcaaaa caccacgccc gaacaaatga aaatgttcct gacccgcatc ggcttcggcg
     9721 cgaaagccgt cattaccggc gacaccagcc aaatcgacct gcccaaaaac atcaaatcgg
     9781 gattgaaaga cgcacgtgag aaactgcacg gcgtggcagg actgtatttc cataccttca
     9841 ccggcgaaga cgttgtccgg catcctctgg tgcaaaaaat cgtcgaagcc tacgaatcgg
     9901 cagaacacga ctgacactcc aatgccgtct gaaacccgat gcatggaaaa tcgggcagag
     9961 aaaaacagag acggaagcca agctccgtat ccgtttcttt atccgtccaa ccgatacatc
    10021 aatcgaattt tctgcatcgg attgcctcat ctccgcaaaa cattgccgaa gtttcgcggt
    10081 tgcacgcctg acgcactttc tgccgcgcca cgccttcatt ccgatcgctt gcctcaaacg
    10141 tcttctgaaa aggcgtaacg ctgcatacat acatggtgga atttgaaccg aaaaggtcga
    10201 ttttcacggt attgccgccg ctttccaata cggcgacacg gtgttccaac tgcgccagac
    10261 gtgcttccaa atcgctgatt ctgtcggcaa atgcaggcat ggcggcacac gcggtcaaaa
    10321 tgcccaaaat acattttttc atcaattttt ccttgttgct tgaaccctgc ccacccggac
    10381 atcctttttc cgggcggcag ggtcagactt tatttgcagg gatgaagccc cttacaaatc
    10441 agaataatac atgaatcggc actttatgct tcatcctatg tgttgaaact tcattgctcc
    10501 ttgtctgttg accgttattc ccaatccgtt cccacaacct tcggcttggg tttgcgcctg
    10561 cgacggcggc gttttttctc ctgtccttcg tttttttcgt gccgggcggc ggcctcgttt
    10621 ttggtcattt ccgtccgctg ttccgccgat gccgtctgaa acgccgtcca ccactcggca
    10681 agtgcgcggt ccgcattgcc ggtttcggcg cgcaagagca ggaaatcata ggcggcacgg
    10741 aaacgcgcct gtgcaaacag tttgtgcgga cgtgcgcctt tgcggttttc aaactgcggc
    10801 tggaacatcc aaatttcacg catcgtggcg gaaaagcgtt gcggtacgcc ccaaccgcgt
    10861 tcgacggttt cgcgcatcgt attgattgca tcggacaggg cgggcgtagg tttcaaaccc
    10921 tgttgcagat tgcttttcca atggcggtcc aactctggcc acatcagggc cgccagtacg
    10981 aaaccgaccg aaaccgattt gtcggcacgc agccgctcat cggtattttt cagggcaagc
    11041 gccgtcattt ttccggcgat gccgtctgaa acgcgcaaag cattgagcag cggatggatg
    11101 tccggtatgt caaatccgtt caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc
    11161 agtttcatga tttcgtcgaa cagccttgcc acaggttcgt gcttcagacg gcatatcgat
    11221 tcggcaatcg gtgcggcggt ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg
    11281 atggcgcgca aaatcctgac agggtcttcc tgatagcgtt cggcggcatt accaatcata
    11341 accagcctgc gggcggcaac atcggcaatc ccgttgtgga aatccaaaat ctcttctttt
    11401 tcaggatcgt aatacaaggc attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg
    11461 ctgccgtagg tgttgtcctt catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg
    11521 ccgcgaaacg tcgttacttc gataatctcc gcaccgttca tcacatggac aatctgaaaa
    11581 cgcctgccga tgatgcggct gcggcggaag agtttgtgca cctgttcggg cgtggcatca
    11641 gttgcgacat cgaaatcttt gggttcgatg ccgagcagca ggtctctgac cgcaccgccg
    11701 accacataag cctgaaaccc cgcccctttc aggcggcgta tgacgttttc ggcggcaaag
    11761 ctcaacattt cggcacggat gttgtgtctt tcggcaggaa tgaccgtttt actttccgct
    11821 tttttactgc tccgaccgga aggcagcatc ttattcaacc attttttcag cataaatatt
    11881 ctgacccgaa gagcggcatc tccacgggca accgtgttca gactgcatca ggcggtacga
    11941 ttccacaaga tgccgaaaac cataagcaaa aggcgtagaa actacgccgt ccgaatcacg
    12001 ccgccctcgc ggcggcaacg cgtcattata acagattgcc ccttaaaaaa tcagaccctg
    12061 cttttgtggc ggagtctgaa atttgatgca ccttatatat tgcgtcccta agaagggatg
    12121 attaacaaaa attaacaccc tttactttct tttccgattg ggaacattcc cgtcgtacaa
    12181 acaagcaaat gaaggaacag aaatcacatt caaacggcgt tccgccgcct ctacgccgct
    12241 tcccagtagt cgatataaag ctgcaattcg aggttgtcgc gccattcgtt ggcaacgggg
    12301 cggtacaccg tgcggatgta ttcgggaatg tcttcgctgc aacgccagaa catcgcttca
    12361 aattcgcagc cgtctttttg caaccacgct tttttgtgtt tgccttccgc gcccaaaggc
    12421 tgctggcgaa cgacgtggaa ctcgtcggta aagctcggcg gcgcaaagcc ctgcccccaa
    12481 acgtggcagg cgaggttttg cgcctgttcc aacgtgatgt cgcaggcggg caggctgccg
    12541 tcagtaatat aggtttgcga caaatcgtct tcgcacacca tctcacgcac ggcttcttca
    12601 aaggccgtct gaaacgcggg aatattgtgt tcaagtatgc ttaaacccgc cgccatcgcg
    12661 tgtccgccga atttcaaaat caaatcggga tggcgtttgg acaccaagtc caaagcatcg
    12721 cgcaagtgca gattgggaat ggagcgtccc gaaccgcgca cttcgccgtt gtccgccggc
    12781 gcaaacacga tggtcggacg gtaaaaacgg tctttgaggc ggctggcaac aatgccgacc
    12841 acgccttgat ggaagtcgtc gcgatacgcc accaaagtcg tctgacctga aggcagggtt
    12901 tcgggaaagg cgttcaacgc gtctcgcagc atagactgct cgatttcgcg gcgttcgata
    12961 ttgaggttgt ttaaccgagc cgccagttcc tgcgcttcgg aatcatctcg cgccaacagg
    13021 caggcgatgc cgaccgacat atcgtccagc cgtccggcgg cgttgatgcg cgggcccaac
    13081 gcaaagccca tatcaaacgg ctgagccttg cgccaatcgc gccgcgccac ttcaaacaag
    13141 gcgcggatgc cggggcgcat tttgcccgaa cgcatccgtt tcaaaccttg cgacacgagg
    13201 atgcggttgt tgtggtcgag ggggacgaca tcggcgacgg taccgagtgc gaccaaatcc
    13261 aaaaggccgc ccagattcgg ctctttgatg ccgtctgaaa aataattgcg gcggcgcagt
    13321 tcggcacgca acgccgtcaa cacataaaaa atcacgccca cgcccgccaa gcttttgctt
    13381 ggaaaaccgc agcctttttg gttcggattg acgatgatgc agtcgggtac ggtatcggct
    13441 ggcaaatggt ggtcggtaac gatgacatcc aaacccagag cctgcgcgcg cgccacgcct
    13501 gcaatactgg caatgccgtt atccaccgta atcagcaaat ccacgccttg cgcggcggcg
    13561 atttcggcga gttcgggcgt taagccgtag ccgtgctcaa agcggttggg cacgaggaaa
    13621 tccactttcg cgcccatcgc cgccaaaccg tccaaaccga cggcacacgc ggtcgcgccg
    13681 tcggcatcat agtcggcgac aatcaggatt ttttccttgc gttcaaccgc atccgccaaa
    13741 cggccggcgg cggcttcgca attcgtcagc gattgataag gcaggaggga agcgagtttg
    13801 tcgtccaact cggcaggact ttgcacaccg cgcgaggcac aaagccgggc gattaaagga
    13861 tcggcgccgg cggcgagcaa atgattgaaa acgtcggtat tgacggggcg ggtttggatt
    13921 ttgactgaca tagcgcgggg ttttacagtg cgggtgtgct tgaaatgccg tctgaaggct
    13981 tttcagacgg cattgccgtt catttgtctg aggaattcgc ggctgcaatc agagccatag
    14041 gcaagaccag caccgcggtc gccgcatcca atatcaacaa ggggggcgta tataagatat
    14101 ttccaaacag cttggatttg tcggtatgtt tttcagtaac cgtataataa atatcggcgg
    14161 gcacactttg ctcaaaatga taatcggcgt tcagtttttg cggcgtggcg tagtatttgc
    14221 ctttggcgga tacgcagcgc gtgtaaatgg tccgattgtc gagtttgacc gctttaaact
    14281 caagctgttt cagcttggcg atgtcgtcag gtctgccggt atcatagcgc aggcaaagac
    14341 ctccggtact gaaattctgg ctgccgggcg cttcgaattt gaccggcagg gcttggtggc
    14401 gggcatagct cggggtatcc tcaactattt ggaagggctt gtccaacccg gccttcaaaa
    14461 ggcccgtcag cttcgccgaa tcttcgggat tgacggcgaa ccagtatttc ccgcccatca
    14521 tcaccaggct gcccttttcc aattgggcat tgtcttcggc aaccacaccg aaggcgcgga
    14581 tttggtcttt gtcaacgtgt ttgcgggtga ttgtttggct gaccgggttg ttcatccccc
    14641 gcaacatcat cgtacagccg ttcagtatca aggttgccgc caaaacggcg gcggtcgttt
    14701 tacggaacaa cattttatct tccaatctaa ataacggcgg atgccgtctg aaaatatgcc
    14761 taaatttccg cgcccccgtt ttccatcacg gttttcaatt cttgaaacag ggcgcggaag
    14821 tttttcggcg gtttgtcctg ttcctgttct tttttggtgt tgcggacgag cgtcctcagc
    14881 ctgcccgcgc cggcgtttgg aaaatcggac ataaactgcg tcagcgcgcc ctcgtctgcc
    14941 aacaaccgta cacgcgcctg ttccacgcgt tgcaaaaagg cgttgtgcgc cgcatcgtcg
    15001 ccgcgcagct tggcaaggaa cgcctcgatg ggcgcgggat cggtatcgcg catcagacgt
    15061 ccgataaatt gcgcctgacg tttgagcgcg ccgttggacg taattttttt ataggtaacg
    15121 acggcctcgt acaaatcttc atccaaaccg atttttttca gcgtgtcgtt tgagagcctg
    15181 gtcagttcca tacccaaatc ctgcaaaccg ttcatctgct ttttcatttg ggttttgctg
    15241 atccactcgt cttcttgttc aaacatcttg attctcgaat tttttccaat agcgtatttt
    15301 aacagaatgc cgccgcgttc agacggcttt ttgcaccgca cgccctgcaa gcggcacgct
    15361 tttgcccctg ccgcccgaaa cggttaaaat gccgcaacgc tccaccaaga aagattgtta
    15421 tgctgttcaa ccacaccgct tccgaactcc tcgacctctg ccgccgcacg ctcgacttgg
    15481 caaaagcagc gggcgcgacc gccgccgaag ccgacttcag cgaatcgctc ggacaaagcg
    15541 tcagcgtgcg cttgggcgaa atcgaacaaa tcgagttcca gcaggacaaa tcgctggaca
    15601 ttaccgttta cgtcggcaaa cgcaaaggcc gcgccggcac cgccgacttc tccgaacaag
    15661 ccctgcgcga caccgtccgc gccgccgccg acatcgcccg ccacaccgcc gaagacggtt
    15721 gcgccggact tgccgacccc gagttgatgg cgcaacacat cggcgacccc gacctttacc
    15781 acgaatggga tttggatacg gaagccgccg tcggcttggc aaaacaatgc gaacaggcag
    15841 cgttggacac ggacagccgc atcgaaaact ccgaaggcgc ggcggtgcaa accggacact
    15901 accaatacgt ttacggcaac acccacggtt ttgccgcaca ccggcaaagc acgcaccaca
    15961 gcatttcctg cagcgtcgtt gccgccgacg aaaacggaat gcagcgcgac tactggtacg
    16021 attccgcctg ccgccatccg gatatggaca gccccgaaac catcggtcaa accgccgccc
    16081 gccgcacttt gcgccgactc ggcagccgca gcatcccgac aggcagctac cccgtcctct
    16141 tcgataccac cgtttcgggc gggctgatcg gacacctcgt cggcacactc tccggcggcg
    16201 cgctctaccg ccaaagcagt ttcctgatcg acagcatcgg caaaaaagtc ctgcccgatt
    16261 tcctcaacct gcgcgaagaa ccgcacatcc cccgctcttt ccgcagcagc tattttgatg
    16321 cggaaggtgt tgccaccgcg ccgcgcttcg tgattcaaaa cggcattgtc gaaggctatt
    16381 tcctcggcag ttacagcgcg cgcaaactcg gaatgcagac cacgggcaac gccggcggcg
    16441 cgcacaacct gtatttgaac catacgcacg aaacgcaatc cgacctgctg aaagaaatgg
    16501 gcacgggatt gctggtaacc gaattgatgg gacagggtgc gaacaccatc accggcgact
    16561 attcgcgcgg cgcggcgggt ttttgggtgg aaaacggcgt gattgcctac cccgtccacg
    16621 agattaccgt tgccggacgc ttgcaggata tgtaccgcaa catcgtcggc gtggcggatg
    16681 acgctttgcg gcgttcgtcc aacaaaatcg gttcgattct gattgcgggg atgacggttg
    16741 ccggaagctg aacgccctgc cgatgccgtc tgaagacgaa ggcggcgcgt tttgccccgt
    16801 gcctgcccgt ttgccgtttt atgccgcacc gccttttacc ggcgacattg cacatttttt
    16861 tcatcagcac tgattttccc ttatccgtca aaacactaag ccacttgccc acaacagggt
    16921 acacacaagt tcaggataag gcgggcgggg attggggact tttccgccat cggcaaaacg
    16981 gcagacaaaa acggcgggac attgcctgtc ccgccgtttt caaatataca tttgaccgta
    17041 catcaggaat taagctgcct gaatgttggc ggcctgtttg cctttagggc cggtggttac
    17101 gtcgaaagag acgcgttggc cttctttcag ggttttgaaa ccttccatat tgatcgctga
    17161 gaagtgtgcg aacaaatctt cgccgccttc gtcaggagtg atgaaaccaa aacctttagc
    17221 gtcgttaaac cattttacga taccggttgc cattagaaac ttcctatact taaaaattaa
    17281 caaaatcagc aaaataaggc atacagcaaa cttaaacgtt cagcgtttct acccagcttt
    17341 atatacttgg tcgagtgccg acttttgctt aaccgtcttt ttacatttgt taagacaaaa
    17401 cgtcaagcta tgttttcaaa aaagtgggaa aataggcgaa agtaccgaaa atacaacaaa
    17461 aatgccgtct gaacgcggct ttcaaatggg acacgctatg accgcgcaac accaatccga
    17521 cacgcttttg caccgcctga acaccctgcc gccgaaacgt tacggcgtat ttttattgaa
    17581 cgacgattac accacgatgg aatttgtcgt tgaaatcctg accgaaatct ttatgctcgg
    17641 gcaggaacag gcggtagcgg taatgctctc ggttcatcac gaaggcaaag gcctgtgcgg
    17701 cacttacacg cgcgatatcg cccaaaccaa acagcaacaa gtcatgcagc gggcaaaagc
    17761 cgaaggacat ccgctgcaat gtattgtcga ggagatttaa tatgcttgcc cctgaattgg
    17821 aacagatttt gcagcagctt taccgcgagg cgcgtaaggc tcattatgaa tttatcagcc
    17881 tcgagcatct gcttttggta ctcatcgaag aagatgccgc cgtgccgaac gtcttaaaac
    17941 tctgcggtgc ggatttaaaa gcggtgtccg aacaactcgc cgccagcgtt gccgaaaaca
    18001 cccccctgat tcccgaccac cttttagaca cggtcgaaac ccggcccacg ctcggcttcc
    18061 aacgcgtcat ccagcgggcg atggtgcata cccaatcggc aggcaaaggc ttggccgagc
    18121 ctttggacgt tttggtcgcg ctgatgagcg aaaccgacag ccacgccgtc tatttcctca
    18181 agctgcaatc ggttacgcgt tttgaagttt tgcgctgtat tgcccacggc tctcccgatg
    18241 aagatgaaga cgatggcaac tatttttcag acggcatgga cgacgataat gagaaccgca
    18301 ccaaaccggg caaaaaccct ttatcggcgt acaccgtcaa cctcaacgcc gaagtcaaag
    18361 ccggccgtat cgatcctttg attggtcgca aacacgaaat ggaacggctg gtgcaaatcc
    18421 tgtgccgccg ccgcaaaaac aatccgcttt tggtcggcga agcaggcgtg ggcaaaaccg
    18481 cgctggcgga cggtttggca catcaaatcg tcaacgacga cattcccgac gcgctcaaag
    18541 aggccgaagt gtacgcgctg gatatgggtt cgcttttggc gggcacaaaa taccgcggcg
    18601 actttgaagc gcgggtcaaa tccgtcttga aacagctcga aaaaatcccg cacgccattt
    18661 tattcatcga cgaaatccac accatcatcg gcgcgggcag caccggcggc ggcacgatgg
    18721 acgcgtccaa cctgctcaaa cccgcattgg cgaaaggttc attgcgctgt atcggcgcaa
    18781 ccacttacga cgaataccgt actatttttg acaaagacca cgccctaagc cgccgtttcc
    18841 agaaaatcga cgtggtcgaa cccaccgttg ccgaaaccgt tcaaatcctg cgcggcttga
    18901 aaccgatgtt cgaagccttc caccaagtcc gctacacgca aggcgcactc gaagccgccg
    18961 ccgaactctc cgcccgctac atcaacgggc gcttcctgcc cgacaaagcc atcgacgtaa
    19021 tggacgaagc aggcgcggcg caacggattc tgcccaaatc caaacggaaa aaagtcatcg
    19081 gcaaagcgca aatcgaaacc gtcatcgcca aagtcgcgcg gattcccgaa aagaccgtgt
    19141 cgcacgacga caaacaagtg ttgcaattcc tcggccgcga tttgaaaaac atggtttacg
    19201 gtcaggaaaa tgccatcgat gcgttggttg ccgccgtcaa aatgtcgcgc tccggccttg
    19261 ccctgcccga caaaccgata ggcagcttcc tcttctccgg ccctaccggc gtcggcaaaa
    19321 ccgaagccgc caaacagctt gcctactcaa tgggcgtacc gctgcaacgc tttgatatgt
    19381 ccgaatacat ggaacgccac gccgtatcgc gactcatcgg cgcgccaccg ggctacgtcg
    19441 gctttgaaca aggcggcctt ttgaccgaag ccgtcaacaa gcagcctcat tgcgtgttgc
    19501 tcttggacga aatcgaaaaa gcccatccag atattttcaa cgtcctcctg caagtcatgg
    19561 acgcaggcaa actgaccgat aacaacggta agagcgccga tttccgcaac gtcatcctga
    19621 tcatgaccac caacgcaggc gcagaaagcc tcagccgacc cagcctaggc tttaccgcca
    19681 aacgcgagcg cggcgacgaa atgcaggcaa tcaacaaact cttcacgccc gagttccgca
    19741 accgcttgga cgcgatcatc ccgtttgcac ctttgtccga acccatcatc gtcaaagtcg
    19801 tggacaaatt cctgctccag ctcgaacacc ggctcctcga caaaaaagtc gaagccgaat
    19861 tcacaccggc attgcgtaaa tacttggcag aaaaaggttt cgacccgcaa atgggcgcgc
    19921 gcccgatgca ccgcctgatt caggaaaaaa tccgcaaacc gctcgccgac gaactcctgt
    19981 tcggcaaact cgccgacggc ggctttgtgc ggatagactg ggatgcggcg aaagaagaag
    20041 ccgtattgaa gtttaagaaa agcaaggtca aaataaaaac cgcctccgca taaaatcaaa
    20101 aatgccgtct gaaaggttca gacggcattt tgtcaaatat cggcccctat tccggcaaac
    20161 ttctccacaa acgccgcgat tttttggaag acgctttgtt tcttcgtcag atattgcggg
    20221 ttgagcggac tcattttcgg cagggtttcg gtcagttccg tgccgttttc gctggcatag
    20281 ccgcgtttga gcgagccggt caaatagcgt ttggcggcgg tttcgttcag gccttcggcg
    20341 gcaatcaatc ctgccgcttc gtgccgcatc acctcttgcg cgtaggtgta aaaggtttcc
    20401 agtatggcgg gaacgtcggg cactttatcc aaatccgtat cgttgatgaa atccacaatc
    20461 aggccctctt tggcgcggtg gccgatgctg gcgcggatga tgcggcggat ttcttccacc
    20521 agctccgctt tgcctttgat ttttttgtga tgctcgaaaa ccagttgcag gatgtaatcc
    20581 agattgattt cctgcgattt gagcaaatcc acctcaaaaa ccacatcgtc ccagtcgatt
    20641 tttgactgtt ctttctgttc gcctgctttt tggcggcgca gccagtctcg gatgtcgtta
    20701 taggcggaac ggtagtcctg caccgccctt tcagacggca tcggcacttt ccgcatttcc
    20761 tgcacgtctt cgtcgctcag gtagtatttt tcctgaaacg ccttcatcgc atcttcgtcc
    20821 gccgcgtcca cattctgcaa ttcgcgcagc gcggcaaatt catcgtagtt ctgcaatacg
    20881 ttttccgccc gcaggtattc gccgaagagt ttggcaaaat cttttttgcc tttttccgtt
    20941 tcgattttgt cgggatcggg gaaacgctcg tgcaattctt ttgctacatc cagataaccg
    21001 cgccgtgctt cgccggtctg gctgtcggta tagccgttca tgtattcttc gtagcttttt
    21061 tccagcacta catttttggt gtttttgtcg ccgaagagcg tgatcgcatc aatggttgcc
    21121 tgctccaagt cgcggaagca gacaatgttg ccgaaggttt tggtagcatc gtaaatgcgg
    21181 ttggtgcgag aaaacgcctg catcaggccg tgatagcgca ggtttttatc gacgaacagc
    21241 gtattcagcg tcggtgcgtc aaaacccgtc aaaaacatgc cgaccacaat cagcaagtcc
    21301 acttcccggt ttttcacccg ttttgccaaa tcgcggtagt agttctgaaa ggctttgctg
    21361 tccgtgccga aattggtttt gaaacaggcg ttgtaatcgt tgatggcagc ctgcaaaaat
    21421 tctttcgcgc tgctgtccat cgcttccggt tcaaaggtct cgtcaacaat ttcaccgacg
    21481 gcgttttgct cttcgttggc ggcaaaggaa aaaatggtgg ctgttttcaa cgggtgcagg
    21541 ctgcctgcct gttgtgtttt gaacgcttcg taatagcact tcgccgcatc cacgctgctg
    21601 acggcaaaca tggcgttgaa gcctttgccg cccgcattca gccggtgcgt tttctgcctg
    21661 aaccggttca ggatatattg cgtgatttcg cggatgcgtt cggggtgcag cagggcttgg
    21721 cggttttcgg cggcactcag ttccttctcg tcctgttccg cttccacggc tttgaactgc
    21781 gggcgcacat cgttgtaatc cactttgaat ttcaatactt tttcatcgcg gatggcatcg
    21841 gtaatcacat aagaatgcag ctcccgcccg aacacgcccg ccgtggtttc cgcgcccaaa
    21901 gcgttttcgg gaaaaatcgg cgtgccggta aagccgaact ggcagaattt tttaaatttc
    21961 tttttcaggt ttttttgcgc ttcgccgaat tgcgagcggt ggcattcgtc gaaaataaat
    22021 acaacctgcc ggtgataaac cggcagatta tcttcacttt tcatcaggtt gttcagcttt
    22081 tggatggtgg taacgatgat tttgttgtcg tctttttcca aattgtgttt caagcccgcc
    22141 gtactttccg agccgtttac gctgtcgggc gaaaaacgtt ggtattcctt catcgtctga
    22201 tagtccaaat ccttcctgtc caccacgaag aaaaccttgt cgataaacgc cgattccgtc
    22261 gccagacgcg ccgccttaaa gccggtcagc gttttgccgc tgcccgtggt atgccaaaca
    22321 tagccgccgc tttccggccc gctccaattc ttcgcctgcg ccgagctgtt gattttccac
    22381 aaaatgcgtt cggcggcggc aatctggtac ggccgcataa tcagcagcgt atcattcgca
    22441 tcgaacacgc tgtaatgcag caaaacgctc agcaatacgc ttttctgcag gaacgtggcg
    22501 gtaaagtctt tcaaatcctt aatcggatga ttgtccgacc gcgcccaatt catcgtgaaa
    22561 tcgaagctgt ttttgtcgcg cttggtggtg ttggcgaaat agcgcgtgtc cgtgccgttg
    22621 gaaatcacga agatttgcag gaatttgaac agcgaatttc cgctgttgaa gctctctttg
    22681 ctgtaacggt gcacctgatt gaatgcctcg cgcaccgcca caccgcgctt tttcaattca
    22741 atctgcacca gcggcaggcc gtttaccaac acggtaacgt cataacggtt tgcatgcgtg
    22801 cccgtctgct caaactggtt gataagctgc acatggttgc gggcaaggtt tttcttgtcc
    22861 agcagataaa tgtttttcag acggccgtca tcaaaagcga aatcgtaaat atggtcgtcg
    22921 tggattttgc gggttttatc ggtaatgttt tcagccggcc tgtccagata ttccgtcaaa
    22981 aaccgcgccc attcgccgtc tgaaaacgcc acatcgttca gccgctgcaa ctgggcgcgc
    23041 aggttttcca gcagcctgct ttggctgttc aaatccttgc ggtattcgta cccctgattt
    23101 tgcaaatcgg cgattaactc cgcttccaac cggttttccg attggtagct gcccgactgt
    23161 tcgatttttt catattggtc gagcacgatg aaattcggcg tttcggcgat gggtttggtt
    23221 tcgaggttca tgggtcgtcc tttgcggtgt cgggttttat agtgaattaa caaaaaccgg
    23281 tactgcgttg gctcgcctta gctcaaagag aacgattccc taaggcaccc aagcaccaag
    23341 tgaatcggtt ccgtactatt tgtactgtct gcggctcgcc gccttgtcct gatttttgtt
    23401 aattcactat aaacggtttg atgttcggat tgggctgcct gaaaaagtgc aggctgcttt
    23461 ttcaggcagc cttggggaag gcaagcagct gttcgcggta atattcgtat tgtttgcggc
    23521 gcagggcgat ttcgtgcggc aggccttcgc tgacggagtg ggtcagggta tcgaatttgc
    23581 ctaggatggc gacgattttt tcctgttcgg ggagcggggg gatggggatt ttatattgca
    23641 taatcgctgc tttgctgcca cgcggcattt ttgcaccttt gg
//
LOCUS       DAXFZR010000027        23305 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000027 DAXFZR010000000
VERSION     DAXFZR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23305
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.027"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..173
                     /locus_tag="ACFJAS_000898"
     CDS             <1..173
                     /locus_tag="ACFJAS_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFC3982182.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1456
                     /gene="cas8c"
                     /locus_tag="ACFJAS_000899"
                     /pseudo
     CDS             170..1456
                     /gene="cas8c"
                     /locus_tag="ACFJAS_000899"
                     /inference="COORDINATES: protein motif:HMM:NF021243.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFJAS_000900"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFJAS_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="ACFJAS_000901"
     CDS             2263..2631
                     /locus_tag="ACFJAS_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982183.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHGPYDKDEWIEKIFCL"
     gene            2638..3111
                     /gene="cas4"
                     /locus_tag="ACFJAS_000902"
                     /pseudo
     CDS             2638..3111
                     /gene="cas4"
                     /locus_tag="ACFJAS_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
     gene            3289..3474
                     /locus_tag="ACFJAS_000903"
                     /pseudo
     CDS             3289..3474
                     /locus_tag="ACFJAS_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(3514..5442)
                     /gene="rpoD"
                     /locus_tag="ACFJAS_000904"
     CDS             complement(3514..5442)
                     /gene="rpoD"
                     /locus_tag="ACFJAS_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC3982184.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETRAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(5629..7401)
                     /gene="dnaG"
                     /locus_tag="ACFJAS_000905"
     CDS             complement(5629..7401)
                     /gene="dnaG"
                     /locus_tag="ACFJAS_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC3982185.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(7546..10296)
                     /gene="secA"
                     /locus_tag="ACFJAS_000906"
     CDS             complement(7546..10296)
                     /gene="secA"
                     /locus_tag="ACFJAS_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC3982186.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            10428..10850
                     /locus_tag="ACFJAS_000907"
     CDS             10428..10850
                     /locus_tag="ACFJAS_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC3982187.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            11133..11684
                     /gene="azu"
                     /locus_tag="ACFJAS_000908"
     CDS             11133..11684
                     /gene="azu"
                     /locus_tag="ACFJAS_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC3982188.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            11779..12288
                     /locus_tag="ACFJAS_000909"
     CDS             11779..12288
                     /locus_tag="ACFJAS_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC3982189.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     EIMAARRCK"
     gene            12322..12942
                     /locus_tag="ACFJAS_000910"
     CDS             12322..12942
                     /locus_tag="ACFJAS_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HFC3982190.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            13055..14200
                     /gene="dapE"
                     /locus_tag="ACFJAS_000911"
     CDS             13055..14200
                     /gene="dapE"
                     /locus_tag="ACFJAS_000911"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC3982191.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGSLTVKGKQGHIAYPHLAVNPIHTFAPALLELTQ
                     EIWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAEPCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            14620..15435
                     /locus_tag="ACFJAS_000912"
     CDS             14620..15435
                     /locus_tag="ACFJAS_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3982192.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTFDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADMAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(15413..15970)
                     /locus_tag="ACFJAS_000913"
                     /pseudo
     CDS             complement(15413..15970)
                     /locus_tag="ACFJAS_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            16320..17072
                     /locus_tag="ACFJAS_000914"
     CDS             16320..17072
                     /locus_tag="ACFJAS_000914"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC3982193.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            17263..17338
                     /locus_tag="ACFJAS_000915"
     tRNA            17263..17338
                     /locus_tag="ACFJAS_000915"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17296..17298,aa:Asn,seq:gtt)
     gene            17469..18479
                     /gene="waaF"
                     /locus_tag="ACFJAS_000916"
     CDS             17469..18479
                     /gene="waaF"
                     /locus_tag="ACFJAS_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC3982194.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKKLHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISIETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            18534..18980
                     /gene="smpB"
                     /locus_tag="ACFJAS_000917"
     CDS             18534..18980
                     /gene="smpB"
                     /locus_tag="ACFJAS_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC3982195.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            19917..20624
                     /locus_tag="ACFJAS_000918"
     CDS             19917..20624
                     /locus_tag="ACFJAS_000918"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3982196.1"
                     /translation="MASLRPTLNQLSSWQEHLYSDVDPFHYIEVTDNSHVIGQTISLD
                     EFHLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDLKDKV
                     PETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIY
                     KLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWL
                     RVIDGHSNQWVQGKQHL"
     gene            20642..21382
                     /locus_tag="ACFJAS_000919"
     CDS             20642..21382
                     /locus_tag="ACFJAS_000919"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3982197.1"
                     /translation="MQLGGEVFTWQNESNQLSIGLMGGQAEQRSTFRNPDTDNLTTGN
                     VKGFGAGVYATWHQLQDKQTGAYVDSWMQYQRFRHRINTEYATERFTSKGITASIEAG
                     YNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAQVNLLGSRQLQSRVGVQ
                     AKAQFAFTNGVTFQPFVAVNSIYQQKPFGVEIDGDRRVINNKTVIETQLGVAAKIKSH
                     LTLQASFNRQTSKHHHAKQGALNLQWTF"
     gene            complement(21365..21670)
                     /locus_tag="ACFJAS_000920"
     CDS             complement(21365..21670)
                     /locus_tag="ACFJAS_000920"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982198.1"
                     /translation="MDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKA
                     VVINAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(21711..23078)
                     /locus_tag="ACFJAS_000921"
     CDS             complement(21711..23078)
                     /locus_tag="ACFJAS_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC3982199.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
BASE COUNT         5477 a         5569 c         6374 g         5885 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgccgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatgcctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcgacga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtaccgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aagcactcgg cattgagtct gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 cattaccgcg tggatgtgta aaaacttctt cgatatccga accttcggcg ctgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacacgcatg gcggtaacca acgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgtgg tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctcagccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacaccg taaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catggtccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgcgcaggcg
     2761 tgggcggaga actgtttgac cgcgcagggc aaggcactgc atgagcgggt ggattcgggc
     2821 gagcccgaaa cgcacaaggg cgtgcgtttt gagcggacgg tacacgtttc ggcggaaaaa
     2881 ccgggcatca gcggcatatt ggatttggtg gaagcggata caaaaacagg ccgtctgaaa
     2941 cctgtggaat acaaacgagg caagcccaaa cctgacccgg gggatgaaat ccagctttgc
     3001 gcccaaggtt tgtgtttgga agaaatgacg gggcaaaccg tttcagaggg cgcgctgtgg
     3061 tatatgcaaa cccgccaccg tgtccccgtc gtgttttcag acggcctgag acctttgcaa
     3121 taacataggt tactaaaatt ttatgctcaa tctcattttc aaaatgcaaa acttttctga
     3181 tttttcctac tttttgctca atattaggaa ggttttaggc aattgaaaat tttttggcgc
     3241 atttttatgc gtcaaatttc gttaacagac tatttttgca aaggtctcgg cctacgcgcc
     3301 caaaccctcg caactatagc cgccgtccgc gaactcttaa acagcggaca aaccccgccg
     3361 cccgactacg gcaaacgctg caaagcctgc tctctggtgg aaatctgtca gccggagttg
     3421 ttggcgaaac gggatgggag tgtgggatat gtggaagcgt tgtttattgt gtaagaaaaa
     3481 accgcaggta tttgaaacct gcggtttttt ggtttacagc ttgctgtctt cgctgtccaa
     3541 gaaacttctc aaacggtcgc ttcttgtcgg atgccgcagc ttgcggagtg cttttgcctc
     3601 gatttgacgg atgcgttcgc gcgttacgtc aaactgtctg ccgacttctt ccagcgtgtg
     3661 gtcggtgttc atatcgatgc cgaaacgcat acgcaggact tttgcctcac gcggtgtcag
     3721 gctttcgagg atttctttgg ttacttcgtg caggctggtg tacattgccg catcggccgg
     3781 cgcaacattg ttggcatctt cgatgaagtc gcccaagtgc gaatcgtcgt cgtcgccgat
     3841 gggggtttcc atcgaaatcg gctctttggc gattttcatg attttgcgga ttttgtcttc
     3901 gggcatctgc atcagttcgg caagtttggc ggaatcgggt tcttcgccgg tttcttgaag
     3961 gtgttggcgc gagatgcggt tcatcttgtt gatggtttca atcatatgta ccggaatacg
     4021 gatggtacgc gcctgatcgg caatcgagcg ggtaattgcc tggcggatcc accaggttgc
     4081 gtaggtggag aatttgtagc cccggcggta ttcgaacttg tcgaccgcct tcatcaggcc
     4141 gatgttgcct tcctgaatca aatcaaggaa ctgcaagcca cggttggtgt attttttggc
     4201 aatggaaatg acgaggcgca agtttgcctg aatcatttcc tgtttggcgg ctgcggtttc
     4261 tttttcgctc gacaccatat ttttgttgat ttctttcaac tcttcgatgg aaatgcgggt
     4321 ttctttttcc atatccgcca actcggtttg tttttcgagg atggcgtggc ggaagcggtc
     4381 gagtgcgttg ctccaaaccc tgcctttggc gatttcttct tcaatccatt gcagattggt
     4441 gatttcgggc aggaagtttt ggatgaagta gtcgcgttcc atatggacgc ggtcgaggca
     4501 gatgtcgcgg atttcgcgtt cgagtttgcg gatgttttct acttttccgc gcaggctgct
     4561 gctgaggctg tcgatttgcc gggtggcgaa acggacttcc agcagtttgt tggcaatcgc
     4621 gtcgcggtag gcgagatagt ctttgtgccg gctgtggtgt ttttccaaac agccgatcat
     4681 ttttttgtag tctttttcga tttgggcaaa gtggccgatg actttttgtt tcaattcggc
     4741 gagattggct gccgagactt catccgcatc ttcttccgat tcttcgtcgt cttcgttttc
     4801 gtccgaattg tcgttggaag gtttctcggg tgctctggtt tccaagtgcc ccaagcccaa
     4861 ttcgttgagc aatacttcat tcgggtcgat aatggcttct acgacttcgt cgacgcggat
     4921 ttcgtctttg cagatttttt cgatgagttc taagatttcc gcaatggaac ccgggcaggc
     4981 ggagatggcc tgaaccatat ttttcagggc gttttcgatt tttttggcga tgatgatttc
     5041 gtcttcgcgg gtcagcaggt cgacctgtcc catttcgcgc atatacatac ggacggggtc
     5101 ggtcgtcctg ccgaactcgg aatctgcgct ggaaagggcg gcctcggctt cttcgacggc
     5161 atcatcgtcg gtaacggcgg cattgtcgct taacaatatg tcttccgcat caggggcgtg
     5221 ttcggtaact tggataccca aaccggaaat catactgacg atgttgtcga tttgatcggc
     5281 atcggacata tcgtcgggca gtgcgtcgtt gatttcggag taggtgatgt agccgcgttc
     5341 tttgcccata atgatgagtt ggcgcaggcg cgcacgttgt tcctcaatcg tcagggggcg
     5401 ggcatcgtct tggtattctt cgtgattttg gtttttggac attgtctgct ttctgttggc
     5461 aagatgccga cggtttgcgt ttggacgcac ctgaaggcgt ggcaaagtag ggtactctga
     5521 tgccgtctga aggctttttg ggatgggtgt tctgcaggtg tcgttgttgc agccggggtg
     5581 aatgcttttc agacggcata acggtttaca gatggcggaa tccgccggtc aattttgttt
     5641 tgcggtcagc agcgacagca aaagtttttt ctcgctttca tttaagccgg attgcaggct
     5701 tttttgtttt aatgtttcaa tttggctgta ttttaactca ttgagcagtt ttttgatgcc
     5761 gatttggaag ttttcgcaat cttcttcgcc gccgccttcc atttcttccg attgaagggc
     5821 cgaccggaag atgcggttga ttgtttcttc gtagggcgaa ccgcgcatat gttccaaaac
     5881 ctgtgcggtt gccggcacgg aaggatggtt tttaatggtt tcggcgaggt tggcaaggca
     5941 ggcgaaatcg ccgtccaacg ccagataatc gggcaggtct atatatgcag cccaatccgg
     6001 atttatcaag aggctgcgga tttgccgttg caccaatgtc ggcataacgg gctgtttgac
     6061 ggaaatcgga ggcagtttgt agtttttttg tttgacgtgc cgctttggcg cttcctgtcc
     6121 gagcagttgc gcgaggttgt cggggtcgat gccgactagc tcgctaagcc gttgttttaa
     6181 caaataagcc aatgccggtg cggtaatctg caccaaaagc ggcgaactgg ttttcaccaa
     6241 ttccgccttg ccttcctgcg tattgagatg aatgccgtct gaaaggtgtt cccagaaata
     6301 ttccgacaaa ggcttgcttt gattcagaag cgcgtcttca aattgcgctt tgccgtaggc
     6361 gcggatgtag ctgtcggggt cgtgttcttc cggcaggaat aaaaaatgca gcgatttgtc
     6421 gtctttcaac tgcggcagcg cgttttccag cgcgcgccaa gccgcttttc gccccgcgct
     6481 gtcgccgtcg aaacagaaat aaatactgtc cgcctgccgc atcaggattt tgacgtgttc
     6541 cgccgtcgtc gccgtgccca aggccgccac accgtagccc acgccgaact gtgccagcgc
     6601 gaccacgtcc atatagcctt cgaccaccaa aatccgttct gcctctttta cagcagcacg
     6661 cccctcatac aagccgtaaa ggtttttccc cttatcgaac aaaggcgtat cgggcgaatt
     6721 caaatatttc ggtttcgagt cgtccagcac cctgccgccg aaaccgatga cctgcccgcg
     6781 cggattgcgg atggggaaca taatccgatg gcggaagcgg tcgtaatgcc gcccctcatt
     6841 gtcaatcacc atccccgtat ccaccaacgc ggtattcgga tacggttgga acacttgcgc
     6901 caaaggctgc cagccgtcgg gcgcgtagcc caaaccataa tgcgcgatga cttccgcgct
     6961 caagccgcgt ttgtccaaat aagctttcgc agccggattg aatttcagct gctgcgcgta
     7021 aaaatcagct gccgccgccg tcgtttcttc cagcgtctgc tgttttttct tgcgctcggc
     7081 acggacttcg ggattatcgt tctgcccgcg cactttaggc acaatcatac ctacgcggtc
     7141 ggcaaggaac tgaaccgcct ccggaaacga cagtccctga tgttccatca caaaaccaat
     7201 cgccgaacca tgcgccccgc aactgaaaca atgataaaac tgcttggtcg gactgaccga
     7261 aaacgacggt gttttttcct tgtggaacgg gcaacacgcc atatagttcg ccccgccttt
     7321 tttcagcgga acctgctcgt cgatgatgcc gacaatatcg gttttggcca gaagctcgtc
     7381 aatgaagtcg gatggaatca tgatgtgggg ggacgggatg tctgttttcg gtacggacgg
     7441 tacgaaatcg ggatttttcg gaatggcggt cggatgtggc ggttcaggca aaatgccgtc
     7501 tgaagccgga aaccggatgt tcagacggca tttgcgttca aacgcttaag ccaatttgcc
     7561 gtggcattgt ttgtatttca aaccgctgcc gcaggggcag gggtcgttgc ggtggacgat
     7621 ttgtccccgc gcttccaagg cttcggggct gaaatccgtc ccatcgggat taaaggcttc
     7681 ggtaaccaga tcggtttgcg actgacccaa aagttcttct atatcgggcg attcggaatg
     7741 gatggactgg atgttgccga cgggttgctc ttcaaccgcc gcgacagggt tttgttcgat
     7801 ttgaaccgaa gtaagcaggg aggcgatatg gaatttgatg ccgttccaca ggtcttggaa
     7861 catggtaaag gcttcgcgtt tatattcctg cttcggattt ttctgggcat agctgcgcag
     7921 gtgtatgcct tgtcgcaggt agtccatggc ggcaagatgt tcgcgccatt ggttgtcgat
     7981 ggcctgcaac atcacgttgc gctcgaaatc agccattgcc tgcttgccga ccagttcggt
     8041 tttggcggca tattcgtttt cgatacgttc gatcaggcgc tctttgatgt cttgaccgtc
     8101 aatcgcattg tccgccttca gccaggattg gatgtcttcc tgaagcctga attcggcagc
     8161 caggcggttt tccagtgtcg ggatgtccca ttgttcttcc atgctgtcgg gcggcatata
     8221 ggtatccacg aggtcgctga cggcatcaga acggatttcc tgcatcaggt cgccgatgtc
     8281 tttgctggtc agaatttcgt tgcgctggct gtaaatgact ttgcgctgtt cgttggcaac
     8341 gtcgtcgtat tccaaaacct gtttgcgcat atcgaagttt ctgccttcga ctttgcgttg
     8401 cgccccttcg atttggcgcg tcagcaggtt gtgctcgatg gcaacgccgc gttcgggggc
     8461 gaggcggttg aggatggcgg cggcgcggtc gagtgcgaag aggcgcaaca gcgggtcttc
     8521 aaacgagagg tagaaacggc tggatccggg gtcgccctga cggccggaac gtccgcgcaa
     8581 ctggttgtcg atacggcggc tttcgtggcg ttccgtaccg atgatgtgca aaccgcctgc
     8641 ttccatcact ttatcgtgtt ctgcctgcca gccgttttcg agtgcggcga tttgtgcctg
     8701 tttctcttcg tcgctcaagg tttcgtcggc gcggatggca tcggtttggt gcttcaggtt
     8761 gccgcctaaa acgatgtccg taccgcgtcc cgccatattg gtggcgacgg taattgcgcc
     8821 gactttgccg gcttgggcga caatcagggc ttcgcgttcg tgttctttgg cgttgaggac
     8881 gttgtgcggc agtccggctt tttgcagaag gcgggagacc agttcggagt tttcgatgct
     8941 ggtcgtaccg acgaggacgg gctgcccgcg tttgtggcat tcctcgatgt ctttgacgac
     9001 ggcttcgaat ttttcttcgg cggaacggaa aatctggtcg ttgaagtctt tgcgctgtac
     9061 ggggcggttg gtcgggatga tgacggtttc gaggttgtag atgctttgga actcgaaagc
     9121 ctcggtatct gccgtaccgg tcataccgga gagcttggtg tacaggcgga aatagttttg
     9181 gaaggtgatg gaggcgaggg tttggttttc gcgtttgatt tccacgcctt ctttggcttc
     9241 tacggcttga tgcaggcctt cggaccagcg gcggccggac atcagacggc ctgtaaattc
     9301 gtcaacgatg acgatttcgc cgtcttggat aacgtaatgt tggtctttgt ggaacagcga
     9361 gtgcgcgcgt aatgctgcca taaggtggtg catcagggag atatttgccg ccgagtacag
     9421 cgagtcgttt tctgccagca gccccatttg ggtcaggatt tgttcggcgt gttcgtgacc
     9481 tgtttcgctc aggatgacct gatgtgcctt ttcgtcgacc caatagtcgc cttcgccttc
     9541 ttctgtctct tgacggacga ggtggggcgg aacggtgttc atgatttggt agagctggat
     9601 gttgtcgtct gcctgaccgg agataatcag cggggtgcgc gcttcgtcaa tcaagatgga
     9661 gtccacttca tcgacaacgg caaaattcaa ttcgcgctgc actttgtcgt attggtcggt
     9721 taccatattg tcgcgcaggt agtcgaagcc gaattcgttg ttcgtgccgt aggtaatgtc
     9781 ggcagcgtag gcgttttgac ggtcgaacgg ctgcatatct gaaataatca cgccgacggt
     9841 cagaccgagg aaattgtaga gcggctccat aatgcctgcg tcgcgcgagg cgaggtagtc
     9901 gttgacggta acgacgtgca cgcctttgcc ggccagcgcg ttgagataga cggcgagggt
     9961 ggcgaccagg gttttgcctt cgccggtacg catttcggcg attttgccgt cgtgcagcac
    10021 cataccgccg ataagctgca cgtcgaagtg gcgcataccg agtacgcggc gggacgcttc
    10081 gcggcagacg gcgaaggctt cgggcaaaat gccgtccaaa gtctgaccgt cggcgaggcg
    10141 ttgtttgaat tcggcagttt tggcttgcag atcagcatcg cttagggctt gcatctgttc
    10201 ttcgagcgcg ttgattctgg caacggattt acggtattgt ttcagcaagc ggtcgttgcg
    10261 gctgccgaag attttcttgg caatgtttgt cagcatgaat aattcctgcg cctgatataa
    10321 tagtggggcg aaaacgcgat tttaacacaa gcgtttgtgc aaagtgctaa tgccgtctga
    10381 aataaaggcg cgtttgcggt atttcaatct tttggggtgt atgttttatg aatttggaac
    10441 agttgggcag gcgggacgcg ctgctttccg gacttttgaa acaggcggga cagtggcggc
    10501 ggttggatgc tgccgtgaaa aaactgctgc ctgccaacct gcacccccac tttcagacgg
    10561 catgtatcga agacggcagg cttgtccttt tggcggcaaa caatatggcg gcatcgcgtt
    10621 tgaagatgat tgccccgtcg gtattgccgc agctggcagg gcttgatgct tcgatacggt
    10681 ctgtttcggt caggctggtt ccaaaaccgg aaaaaccgcc gaaaaccaat accctgcatt
    10741 tgagtaaggc tgcgttggag agtttcgatt cggcggcggc aaagttggaa gaacggcatc
    10801 ccgaattggc ggaggcactg gaggagctgg tcaggaagta cggggcataa ggcggccggc
    10861 agggatgttg taaatatccg ttaatatttg cgcggcgggg gacggctgcc ctaatattaa
    10921 agaaataggt tcggggtaag attgcccttt ttttgggtaa acgattgtaa acttgcaaac
    10981 aggctttgat ttcaaatgaa gtttgtagca aaatgccgcc ccgaaacatc tgtttgtgca
    11041 acacggcgga atctttttca aggttttgtt aatggcggtt gcactttgat ttccgtaaaa
    11101 ccgaatatta ttttatcgat tggagattta ccatgaaagc ttatctggct ctgatttctg
    11161 ccgccgttat cggtttggct gcctgctctc aagaacctgc cgcgcctgct gccgaggcaa
    11221 ctcctgccgg tgaagcaccc gcttccgaag cgcctgccgc cgaagctgct cctgcagatg
    11281 ctgccgaagc ccctgctgcc ggcaactgtg cggcaactgt cgaatccaac gacaatatgc
    11341 agttcaacac caaagacatc caagtcagca aagcatgtaa agagttcacc atcactctga
    11401 aacataccgg tacgcaaccc aaagccagca tgggtcacaa ccttgtgatt gccaaagctg
    11461 aagacatgga cggcgtattt aaagacggcg taggtgctgc cgataccgac tatgtcaaac
    11521 ctgacgatgc gcgcgttgtt gcccacacca aactgatcgg cggcggcgaa gagtcttccc
    11581 tgactttgga tcctgccaaa ttggctgacg gcgactacaa atttgcctgc accttcccgg
    11641 gtcacggtgc tttgatgaac ggcaaagtga ctttggtcga ttaatccgct taaagtttca
    11701 aaagacagac agcctgcttt gtgcaggctg ttttattata aaatgactgc ttgaaaagtc
    11761 cccgttgaga acgaaaacat gaatccgttt gaaacccaaa gcgttacctt tgccgaaccg
    11821 attgaaatgc tgtatgcctg ccacggcaaa gtgcgccgtt tttgcggcca gattgctatg
    11881 ttgtcgggct atatcgccga aaacggctgc aatcagcttg ttttgcaaac catccgccaa
    11941 atctcccggt atttcaacgt tgccgcgccg ctgcaccatg aggacgaaga agaaaacttt
    12001 ttcccgctgc tgctgcaata cgcgccgcaa gcccgtgaag gcgtggacga gctgttgcgc
    12061 caacatatcg ggctgtacga caactgggcg gctgtttccg ccgaatttgc caaactcgaa
    12121 gcagacaacg cttatatccc cgatgcggaa gcgttcaaac gctttgtgga gggatatgat
    12181 gtgcatctgg cgattgaaga gcctttgttc gacatgggca aaacgtttat ccccgaagag
    12241 aagctgaccg aaatcggcga aatcatggcg gcgcgccggt gcaaataatg ccgtctgaaa
    12301 caatcgtctc aaggatatat tatgctgacg cccaaaagct gcgatttgtt caatatcccg
    12361 tttttccagt ttgcccagct caaaaaatac cagcccgaga gcattccgca aatcaaagcc
    12421 gactacaagg aaaactggca gatatggcag cagttgatac agcaggtcgc cgcagatttg
    12481 agcgaacctt tcgccccgcc gcatatcgaa cgctggtgta acggctggca ggttcgcgcc
    12541 catttcttcg cctacttcaa atacgcccaa tacaaaaatt ccgccgccat cctgtcgata
    12601 ctgctcaacc gccgccgcct gagcgtcagc ttggactggc attgctacaa agccgacgtg
    12661 tcgccgattg cgctgcccga gtacaaccgc tggctggatg atttcgatac cgaaaaatac
    12721 gccgcattcg atatgtggca cggcgcggaa agcgaatatg acgactaccg caccgtcgca
    12781 caacaaagcg aatccgaccg aaggttgcaa aacgacgaag actttttctg catcggcaaa
    12841 cacattgagc gcgacgattt gggcaagcag gatgtagcaa aatggatagc cgaaacggtg
    12901 gaagatttac tgccgcttta cgaagcctgt cacggcaaat gattcagaaa actgacgggt
    12961 acgaatgccc gtcttataca ccaatccaac ccggaggtcg tctgaaaatc agcttcagga
    13021 catttcagac gacctgattt ccaaagtgaa caaaatgacc gaaacccaat ccctagaact
    13081 cgccaaagcg ttgatttccc gcccgtccgt tacccccgac gaccgagatt gccaaaaact
    13141 gcttgccgaa cgcctgcaca aaatcggttt tgcggctgaa gaactccatt tcggcgacac
    13201 caaaaacatc tggttgcgac gcggcacgaa agctcccgtc gtctgttttg cagggcatac
    13261 cgacgttgtg ccgacaggtc ctgttgaaaa atgggattcg cccccgttcg aaccgaccga
    13321 gcgcgacgga agattatacg ggcgcggcgc ggcggacatg aaaaccagca tcgcctgttt
    13381 cgttaccgcc tgcgaacgct ttgttgccga acatcccgac catcaaggca gcattgcact
    13441 cttgattact tccgacgaag agggcgatgc actggacggc acgaccaaag tcgtcgatgt
    13501 gttgaaagca cgcggagagt tgattgacta ctgcatcgtc ggcgaaccga ccgccgtgga
    13561 caaattgggc gatatgatta aaaacggccg gcgcggctcg ctgtcgggca gcctgaccgt
    13621 caaaggcaag caagggcata ttgcttatcc tcatttggcg gtgaatccca tacatacttt
    13681 tgccccggcc ttgttagagc tgacgcagga aatttgggac gaaggcaacg aatacttccc
    13741 gccgaccagc tttcaaattt ccaatatcaa cggcggcaca ggcgcgacca acgtcattcc
    13801 gggcgagttg aacgtcaaat ttaatttccg cttctccaca gagtccaccg aaacagggct
    13861 gaaacaacgc gtccacgcca ttttggacaa acacggcgtg caatacgatt tgcagtggtc
    13921 gtgttcggga cagcccttcc tgacccacgc gggcaaactg accgacgtgg cacgcaccgc
    13981 cattgccgaa ccctgcggcg ttgaggccga attgtccacc accggcggca cttcggacgg
    14041 acgcttcatt aaagccattg cgaaagaact catcgaatta ggcccgtcca atgccaccat
    14101 ccaccaaatc aacgaaaatg tgcggctgga cgatattccg aagctgtcgg cggtgtatga
    14161 gaggatattg gcgcggttgt tggctgaaaa ggccgtctga aaagaagatg ggctggttgt
    14221 cggatatgga tgtccgacaa tctgattaga cccaacatga gtttgagata acgcattgat
    14281 ttttatgtag agcgatttat ttgaacggta atccattccc tctcccgtgg gagagggtta
    14341 gggagagggc attgggacgg caacggaaat attgtctgta tctgccacgt tttaccctcc
    14401 ctccgaccct ccccacaggg gagggagcta agttgcaggc acaccgacgg aatatggttg
    14461 aaaccctgcc cttcagggca gtagaatttt ggtttatttt ttgggaagta accccttcca
    14521 aatcagggta aacagggctg cgccttatgt gcggccctgt gtgttgaaac attgaggaat
    14581 ataaccgtgc cgtcaaatta ctttcatacg cgtgaacgat tgggtgcttt aggcggcatc
    14641 gtgcaacatc ataccgcctt gtaccgcagc gtggcggtat atgaaccgac cgtggttatc
    14701 gtgcggcgcg gatgcaagaa gctgcggtgg gcggggcgcg aattgaggat tgcggcgggt
    14761 gaggctgtcg gcttggcagg cgggcagacg tttgacgtca tcaatattcc cgattcagac
    14821 ggcctctatc aggcgcaatg gattgctttc gaacaggaaa ccgtagaaag gttcgccgca
    14881 caatacggga cggcgcaggc agtgtgcgat gcggtgaagc tgccgcaccc cgggcgtatg
    14941 ggcgcggcgt ttgattatgc ggctgcggta ctggcggatg aagaagtgcc gcacaatgcg
    15001 gcggaggccg cgctttgcgg cgtgttggcg tggttgcagc atgacggcat cggttttgcc
    15061 gtgtacggag gcgtcaacct gatgcggcaa atccgcaaac tgattactgc cgatatggct
    15121 gcggactggt cgtcggcaat gctggcgcag cggcttaatt gcagcgaggc cgcgttgcgg
    15181 cggcggttgg cgcggcagga cacgaatttc cgtacgctgc tgacggatgt acgcatgatg
    15241 cgcgcgctga cgctgttgca ggttacgcaa tggtcggtag cgcagattgc cggcgcggtt
    15301 ggctatgact gcccgtcccg tttcagcgcg cgcttcaaag agctgttcgg ttgcgtaccg
    15361 tcggtggtcc gctcggaagc ggagccggcg gcatatcggc gcacggggca ggtcagcgtc
    15421 ggtgttcgac cgtgaatttc gcttcgccca agctgtttgc cttagtggag aaaccgacca
    15481 gcacgggtgt ggattcggcg gtgatgttcg gcagcttggc gactttcagc gcggtaagcg
    15541 tgaattcgta gcggtgtttc ctgccttcgg gaggacacgc gccgccgtag cccggcgcgc
    15601 cgaagtccgt gcgggtttgc agcgcgcctt tgggcggcct gcctcattgt gcggtaatgc
    15661 ctgcgggcag gcggcggaca tcggcgggaa tgtcggcgac cacccagtgc atccggtcca
    15721 gaccgatcgg cgcgtcttta tcgtaaacgg tcaggacgaa actttttgtc cctgcgggcg
    15781 ggtttttcca cgacagcgcg ggcgaagcat tgccgcccga acagccgaag ccgtaaggcg
    15841 cgctcaaaag ctggttttgc gtgaagccgc cgtcttcgga cgggttgtcg aattgcagcg
    15901 tgaatgcgcc cccggcaaag gcagcggttg aaaaagccag cagtacggcg gagaggatgc
    15961 gggtttgcat agatgattcc tttgcttgat gaataagcgg attcattata ggctgtcaac
    16021 ggcggcagga atgtgccgaa acggttggat gatgtgccgg aatgcgcaaa tgcaaaaatc
    16081 gtggttttag acgacccttt gaaggccgtc tgaaattttt catagggcga cgctttatgt
    16141 gtcgtcctgt gtgttgaaac attcctttcc ctgatgcttt ggaaatattg tatatttagg
    16201 cattgccgca accagaccct caaggtttcc gccgcagcct gttcctgtac cggcgtgcgg
    16261 gaatggcgga aatcggggat ttcgccgtca aatttatgat agggatacgg agcaaacgaa
    16321 tgattaccct gccttcctta aacaatctgc cgtcaaaatg ggatgaaatc cgccattgcc
    16381 tcgaaacccg ggtttttgag tgcggcgtta tgccgtatcc caatctgacg gaatgcgaag
    16441 ccgggcagtt cgagcgggat ttttgggaca atatcggctg cgcgcctgaa gaatatgtcc
    16501 gtatccgccg cgccatccgt ctgttggaat tacgttatcc cgacagcttg aacgagctgg
    16561 tgtgtgccgc cattgccacg cctttgggcg agatgttggc ggtgttcggc gttaagggat
    16621 tgtgcctgct ggagtttgtc ggacagaagt atttggaaca ggaaattgcc gccgtccaaa
    16681 aagccttgcg cggacggttt gtgtttcggg aagacgggcg gatgcaattt ttgcgtcagg
    16741 aattggattt gtatttccaa tgccgtctga aaacctttgc cacgcccttg gaaatgattg
    16801 gtacggcgtt tcaaaaacag gcgtgggacg cgcttttggc gattccttac ggcgaaacgc
    16861 gcagttataa ggagcaggcg cagcgtttgg gcaaccccaa agccgtccgc gccgttgccg
    16921 ccgccaacgg gcagaacaag gtgtctgtca tgattccctg ccaccgcgtc atcggcagcg
    16981 acggcaaact gaccggctac gcgggcgggt tgaaccgcaa acagttttta ctggctttgg
    17041 aacgcggcga agttcagacg gcattgtttt aagcggggaa atgcagaacg gctcatcggg
    17101 cttaaatatg gtgtgatgac ggctgtgaaa attcatacaa cggcaatcct tttaaggcat
    17161 cggtcttaat gtctttctga ttcatatgct tgtcgttttg tcgcccattt ttccaaaatt
    17221 aaggattgac acattttttc cggacgctat aatacgcccc attccccgat agctcagtcg
    17281 gtagagcgac ggactgttaa tccgcaggtc cctggttcga gcccaggtcg gggagccaga
    17341 attttgaagc ccgaacttga atgttcgggc tttttcacat ttgcacgtcc ggcgttcgga
    17401 atgcggcgag ataggttttc caacggtttc gcggtaaaat tctgcctgtc tgatttcggt
    17461 ttcgttttat gtctatcaaa atcctgatta tttctcccag ttggataggc gactgcgtga
    17521 tgacccagcc cttgttccgc cgtttgaaga aacttcatcc cggttgcacg attgatgtgt
    17581 tcgcaccgaa gtggtcgatg gcggtgttcg agcgtatgcc ggaagtgaat gagattcttg
    17641 aaaatccgtt cggacacggt gcgttggagc tgaaacgccg ttggcgggtc ggcagggagt
    17701 tgggacggcg cggatacgac agggttatcg tgttgccggg ttctttgaag tcggcagtca
    17761 tcgcgctggc aacgggcatc ggaaaaagaa cgggttatgt cggcgaaagc cgttattttc
    17821 tgttgaacga tatacgcagg ctggataagg aacgtctgcc tttgatggtg gaccgatata
    17881 cggctctcgc gcatcagagt caggaagatt ttgacgggca ttcgggattc cccgagtttt
    17941 ccattgatga acggcggcgg gaaatttcta ttgaaacttt cggtttgaat cttggaaagc
    18001 ctgttctggc tttttgcccg ggtgcggaat tcgggccggc aaagcgttgg ccggcaaggc
    18061 attttgccga gttgggcaaa cactattcgg aggcgggttg gcaggtttgg ttgttcggtt
    18121 cgcaaaaaga tgatgaaatc gccgaagaaa tcaactgcct ttcagacggc atgtgtgtca
    18181 atttgtgcgg caaaaccgat ttgtcgcagg caatggattt gctgtcgttg gcggacacgg
    18241 tcgtgtgcaa cgacagcggg ctgatgcatt tggcggctgc tttgggcagg aaggtagtgg
    18301 cggtttacgg ttcttccagc ccgacgcata cgccgccttt gagcgacagg gcgaaaatcg
    18361 tcagcctgca cttggaatgt tcgccctgtt tcaaacgtga atgcccgttg gggcataccg
    18421 actgcctcaa caggctgtat cccgagaaga ttgtgcaggc ggttgaagag gcggtatgat
    18481 ggcttctgtt ttcggattta taccgtctga cggcggatat tgataggatg tttatggcta
    18541 ttgcgaacaa taaaaaagca tttcacgatt ttttcattga agaccggatt gaagccggtt
    18601 tggtcttgga aggctgggaa gtcaaagcaa tccgcgccgc gcgcgtacag cttaaagaga
    18661 gttatatcta ttggaaaaaa gatgcgtttt atctggtcgg ctgccatatt acggctttgc
    18721 ctacggcttc gacacacatc aaaccggatg ccgtacgtcc gcgcaaactg ttattaaaac
    18781 agtcggaaat caataagtta atcggaaaaa ccgaacgtgc aggttacacc atcgtgccgc
    18841 tggatttgca tttttcacgc ggaaaaatca agatggaaat cggtttggca aaaggtaaga
    18901 aacagcacga taaacgacaa agtatgaagg aagccgactg gaaacgcgag aaacagcggt
    18961 tgattaagca tacgcgctga ttgtggaaac aatgcttcag acggtacggg caaggctgcc
    19021 gcctgatgcg taaacgttct acacctaaac cagcatccgc tttaatcagc acggcatgat
    19081 gccccaaacc ttcgtcaaag gtttgagcaa tttgatgata aattggcatt agccaagggt
    19141 aaagtgcggt catttttcct gaaatttttg taaataacga gggcaattat acacattttc
    19201 aaaccggctt gacaaaatag caaaaagcaa acgtttactt ttaataaata agaaataaat
    19261 agagaaaatt tgaactaaat gacatttttt gttcagtttt atgctaaaat ccacgcccta
    19321 tttattttta cttgacaaag gaaaatttat tatgaaactc gaagcaagca agcagaagtt
    19381 taaaaaatca tttattataa gtctattttt ttctattctt taatacctct ccgcttttgg
    19441 ctgttgatta tgtttacgac aaaaccaagc tcactgatga tgaaattatc cgcttaaaaa
    19501 aaactccgcg atagaaatag tgaatattgg aaagaagaaa cttatcacat aaaaagtaac
    19561 gggcgaactt atccaaacat tcccgcatta ttccctaaac atcctttcga tccattcgaa
    19621 aacatcaaca actcaaaaaa gatttctttt tatgacaagg aatacactga agattacctt
    19681 gttggcttcg cccgaggttt cggggttgaa aaaagaaatg gggaagaaga aaaaccactc
    19741 cggcaatatt ttaaggactg tgtaaatact gagaattcca ataatgataa ttgtaaaatt
    19801 tcatccttcg gaaactatgg cccaatatta ataaagagcg atattttcgc cttagcatca
    19861 caaataaaaa atagccatat caacagcgaa attttatctg taggtaatta tatcgaatgg
    19921 cttcgcttcg cccaacactt aatcagcttt ctagttggca agagcatctc tattctgatg
    19981 tagatccatt ccactatatt gaggttacag acaactcgca cgttatcggg caaacgatta
    20041 gtttggatga attccactta gaaaattctc tctgggaacc ccgttgggat tctgatgttg
    20101 gggagttaaa aacaacgaat gcagacatcc gttttaatac taaaagtgaa agtttactcg
    20161 taaaagaaga ttatgctggc ggagctcgtt ttcgttttgc ttacgatcta aaagataaag
    20221 tccctgaaac accagtttta acttttgaaa aaaatataac tggcacttcc gacatcatct
    20281 tcgaaaatcc aatagacgat ttaaaaagcc tcgacgggca tcaaattatc aaagtaaatg
    20341 gcacagcaga taaacacgca ttccgtcttt ctggcaaaca tcaaaaggga atttataagc
    20401 tctctttaca acaacgccca gagggctttt ttaccaaagt gcaagaacgc gatgatattg
    20461 cgatttatgc acaacaggct caagccgcca ataccttatt cgccttgcgt ttgaacgaca
    20521 aaaacagtga tattttcgac cgcactttac cacgcaaagg cttgtggttg cgtgtgattg
    20581 acggacattc caaccaatgg gtgcaaggta aacagcacct gtagaaggct atcgaaaagg
    20641 tgtgcaactc ggcggcgagg tgttcacatg gcaaaacgaa agtaatcaac tttctattgg
    20701 cttaatgggc ggacaagcag aacaacgcag cactttccgc aatccggata cagacaatct
    20761 tacaacggga aatgtgaaag gctttggtgc gggcgtttac gccacttggc atcagcttca
    20821 ggacaaacag acaggtgcgt atgttgatag ctggatgcaa tatcaacgtt tccgccaccg
    20881 cattaacact gaatatgcta cagaacgttt tacttcaaaa ggtattactg cctcaattga
    20941 agcgggttac aatgcgttat tggcggaaca cttcactaaa aagggcaaca gccttcgtgt
    21001 ttacctacaa ccacaggcgc aattgactta tttgggggta aacggaaaat tcagcgatag
    21061 cgaaaatgcc caagtgaatt tgcttggctc ccgccaatta caaagccgag tgggcgttca
    21121 agctaaagcg caatttgctt tcactaatgg cgtcactttc caaccatttg ttgctgtcaa
    21181 ttcaatctac caacaaaaac ctttcggtgt ggaaatagac ggggatcgtc gagtgataaa
    21241 caataagacg gtaattgaaa ctcaattagg tgtagcagcg aaaatcaaat ctcacttaac
    21301 cttgcaagcc tcattcaacc gccaaacaag caaacatcat cacgctaaac aaggtgcgtt
    21361 gaatttacag tggacgtttt aagttaaatt gtaacaaaat aaaatcggca gggaaattca
    21421 ctgccgattt tattatttta aaagtgcggt catttttcgt gtaatttcca ccaattttta
    21481 ccgcactttc aatttcagct tgaacaagtt caatagtctg ctctgcatta attacgactg
    21541 cttttggatt atcttttact aattctaaat agcgtgctcg agtgcgatgg aaaaaatcta
    21601 aatccatttg ctcaatacgg tctaactcgc cacgtccacg agctcgcgct aaaccgacaa
    21661 tcggatctat tgtccttttc tttgttgtgt atgcaaatca gaaatgccgg ttacggaagc
    21721 agtttgccag gattcataat gttatatgga tcaaggtgtt gtttgatgct tttcatcagg
    21781 gcaatttcag agggcgtgcg gacagcgggc agccattgtt ttttgatgat acctatgccg
    21841 tgttcggcgg caatcgtgcc gttgcgggca aggacgttgc gatagactgt gctgttgatg
    21901 tcgttttcgt aacggtaaac ttcattgctg aggatttcgg gcgggaaagt attgtagtgc
    21961 aggctgccgt cgcccagatg tccgaagcag acgatttgta tgcctttgaa attctgttcc
    22021 aaatctttgg cgcaccggcg gacaaagtcg gcaacgcgcc cgataggaac ggcaatatcg
    22081 tgtttgatgc tggtgcccag tttgcgttgg gaggcggaga tgttttcgcg caacgcccac
    22141 atatggatac gttcttgttc gctttgcgcc aatacgctgt cggtaaatcc ttttttataa
    22201 agaaattcga caagccgatc atcaagattg ctgtcgggta atgagtcggt caactcaagt
    22261 aaaatatgcc attctgaatg tgtcggcagg gggagtttgc tgaattcgga agacaactcg
    22321 gcggcaaaac ggccgatcag ttcaaaactg cataagcgtt cggcaaagtg cgcttgggtt
    22381 tcggtcagca ggcggacggc ggattcgatg tcgggaatgc cgacccatgc cgttgccttg
    22441 tccgagggac gggcaaaaag cttgagcgtg gcggcagtga tgatgcccag tgtgccttcg
    22501 ctgccgataa acagatggcg caggtcgtag ccggtggtgt ttttatgcag gggatggaga
    22561 tgggaaacca gttcgccgtt ggggaggacg acttccaaac cgataaccag gtcgcgcatc
    22621 gtgccgtaac gcaatacgtt caaacctccg gcattgcagg cgatgttgcc gccgatttgg
    22681 cacgagcctt cgctggcgag actgagtggg aacagcctgt ttgaggcttc ggctgcctgt
    22741 tggacggttt ggagtacgga acctgcttcg acggttatgc agttgtctga caaattgatg
    22801 ctgcggatgc ggttgagttt ggaaaggttc agcaatacgc cgttttccga tactgccgcg
    22861 ccgcacaaac cggtattgcc gccttgcggc gtaaccggaa tgcggtgttc gtaacaaaaa
    22921 cgcataatgg tttgcacgct ttcaacgctg cgcggctgca aaatgatgtc tggtgcagac
    22981 gtaaagcggt tgcgctgatc ttttaaaagc gtcggagaag gttcggcaat ttcatctgcc
    23041 ggcaggagtc gggaaaattc ggtatgaagg tcaggcatgt ttgtcggaag ggtagggaga
    23101 agattgagga agggaatctg atgccgtctg aaaaatcaaa aaagcaggat actgaacgta
    23161 tcctgctttt ttgcaagcgg aaaatgctta ttttgcagct tcggcagcag gagcttcagc
    23221 ggcaggtgct tcggtagcag cagcttcggc ggcaggtgct tcggtagcag cagcttcggc
    23281 ggcaggtgct tcggtagcag cagct
//
LOCUS       DAXFZR010000028        23212 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000028 DAXFZR010000000
VERSION     DAXFZR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23212
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.028"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(148..1365)
                     /locus_tag="ACFJAS_000922"
     CDS             complement(148..1365)
                     /locus_tag="ACFJAS_000922"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC3982200.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(1567..2295)
                     /gene="rph"
                     /locus_tag="ACFJAS_000923"
     CDS             complement(1567..2295)
                     /gene="rph"
                     /locus_tag="ACFJAS_000923"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC3982201.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(2377..2841)
                     /locus_tag="ACFJAS_000924"
     CDS             complement(2377..2841)
                     /locus_tag="ACFJAS_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC3982202.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3060..3836
                     /locus_tag="ACFJAS_000925"
     CDS             3060..3836
                     /locus_tag="ACFJAS_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC3982203.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            4081..4935
                     /locus_tag="ACFJAS_000926"
     CDS             4081..4935
                     /locus_tag="ACFJAS_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC3982204.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            4976..6184
                     /gene="pncB"
                     /locus_tag="ACFJAS_000927"
     CDS             4976..6184
                     /gene="pncB"
                     /locus_tag="ACFJAS_000927"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC3982205.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            6276..7994
                     /gene="argS"
                     /locus_tag="ACFJAS_000928"
     CDS             6276..7994
                     /gene="argS"
                     /locus_tag="ACFJAS_000928"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC3982206.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            8546..9964
                     /locus_tag="ACFJAS_000929"
     CDS             8546..9964
                     /locus_tag="ACFJAS_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC3982207.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLVPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWPAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            complement(10417..11163)
                     /locus_tag="ACFJAS_000930"
     CDS             complement(10417..11163)
                     /locus_tag="ACFJAS_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC3982208.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(11147..11326)
                     /locus_tag="ACFJAS_000931"
     CDS             complement(11147..11326)
                     /locus_tag="ACFJAS_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982209.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(11342..12013)
                     /locus_tag="ACFJAS_000932"
     CDS             complement(11342..12013)
                     /locus_tag="ACFJAS_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC3982210.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(12082..12753)
                     /gene="rpiA"
                     /locus_tag="ACFJAS_000933"
     CDS             complement(12082..12753)
                     /gene="rpiA"
                     /locus_tag="ACFJAS_000933"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC3982211.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(12830..13312)
                     /gene="ispF"
                     /locus_tag="ACFJAS_000934"
     CDS             complement(12830..13312)
                     /gene="ispF"
                     /locus_tag="ACFJAS_000934"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC3982212.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDMTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(13344..14033)
                     /gene="ispD"
                     /locus_tag="ACFJAS_000935"
     CDS             complement(13344..14033)
                     /gene="ispD"
                     /locus_tag="ACFJAS_000935"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC3982213.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTIKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLGGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(14030..14764)
                     /gene="dnaQ"
                     /locus_tag="ACFJAS_000936"
     CDS             complement(14030..14764)
                     /gene="dnaQ"
                     /locus_tag="ACFJAS_000936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC3982214.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(14920..16230)
                     /locus_tag="ACFJAS_000937"
     CDS             complement(14920..16230)
                     /locus_tag="ACFJAS_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3982215.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIRLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(16220..16405)
                     /gene="ccoS"
                     /locus_tag="ACFJAS_000938"
     CDS             complement(16220..16405)
                     /gene="ccoS"
                     /locus_tag="ACFJAS_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC3982216.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(16405..16704)
                     /locus_tag="ACFJAS_000939"
     CDS             complement(16405..16704)
                     /locus_tag="ACFJAS_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982217.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            17055..18251
                     /locus_tag="ACFJAS_000940"
     CDS             17055..18251
                     /locus_tag="ACFJAS_000940"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC3982218.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(18301..18423)
                     /locus_tag="ACFJAS_000941"
                     /pseudo
     CDS             complement(18301..18423)
                     /locus_tag="ACFJAS_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18765..20606)
                     /locus_tag="ACFJAS_000942"
     CDS             complement(18765..20606)
                     /locus_tag="ACFJAS_000942"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC3982219.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
     gene            20697..21167
                     /locus_tag="ACFJAS_000943"
     CDS             20697..21167
                     /locus_tag="ACFJAS_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC3982220.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(21240..22052)
                     /gene="hpnC"
                     /locus_tag="ACFJAS_000944"
     CDS             complement(21240..22052)
                     /gene="hpnC"
                     /locus_tag="ACFJAS_000944"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC3982221.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(22139..22621)
                     /locus_tag="ACFJAS_000945"
     CDS             complement(22139..22621)
                     /locus_tag="ACFJAS_000945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3982222.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
BASE COUNT         5537 a         6858 c         5663 g         5154 t
ORIGIN      
        1 tttcaatttg tcatatatag ttaatttttt tagggtattt tattttttgt tccaaagtca
       61 aaatatgccg tccgaacgtt cgggcggcag acaaaacggc actgcccgat aaaggcagtg
      121 ccgttgtccg ttttaaaccg tgaaacatca gcccaaatca aaggctttat gcaataccct
      181 ggttgccagt tccatgtatt tttcgtcaat caatacggaa actttgattt cggaggtgga
      241 aatcatttgg atgttgatgc cctcttcggc gagcgtgcgg aagattttgg cggctacgcc
      301 gacgtgcgaa cgcataccca aaccgactgc ggagactttg cacacggtgt cgtcgccgtc
      361 gatagaagct gcgccgatac tgtctttacg ttccgacagg atttccaaag tctgtttgta
      421 atcgccgcgc ggtacggtga aagaaaaatc ggttgtgcct tcgctgccga cattttggat
      481 aatcatatcg acttcgatgt tggcatctgc gaccgcgccc aaaatctgat aggcgacgcc
      541 gggcttatcg ggcacaccgc gcacgttgat gcgggcttgg tttttatcga atgcgatacc
      601 ggttacggca gctctttcca tgttgtcgtc ctcttcaaag gtaattaagg tgccgttgcc
      661 gccgtcttgc aggctgctca gtacgcgcag gcgcactttg tattttccgg cgaattctac
      721 tgaacggatt tgcaaaactt tcgaaccgag gctggccagt tcgatcattt cttcaaatgt
      781 aaccgtatcc atacggcgcg cttcgggtac gacgcggggg tcggtggtgt aaacgccgtc
      841 tacgtcggta tagatttggc attcgtccgc tttgagggct gcggcaagcg caacggcgga
      901 agtgtcggaa ccgccgcgtc ctagcgtgga aatattgcct tcactgctga tgccttggaa
      961 gccggcaacg atgacgactt tgccggcggc gaggtcggcg cgcatttttt cgtcatcaat
     1021 gctttctatg cgggctttgg tgtgggcggt atcggttttg agggagacct gccagcctgt
     1081 gtagcttttg gcatccacgc cgatatcctt caatgccatt gccaaaaggc cgatggttac
     1141 ttgttcgccg gtagctaaga cgacgtccag ctcgcgcgga tcgggatgct cctgcatttc
     1201 gtgcgccaac gcaaccagac ggttggtttc gccgctcatg gcggatacga cgacgacgat
     1261 atcgtgtcct tcggcgcggg ctttagcgac acgtttggcc acgtttttaa tgcgctcggg
     1321 cgagcctacg gatgtgccgc cgtatttatg tacgattaac gccatgtttc atgctttctt
     1381 gtgtgggatt ggtcgggcag catggtttgc tggaaaagcg gctattatta ctatttttta
     1441 catgaagttc aagaacggac tgcgctttcc cgcctgccgt ttgacagcgg tcagcgaaaa
     1501 acctgttctt tcagattttt gacaaaatgc cgtctgaacg gttttcagac ggcatacgga
     1561 cagtaattaa gcaacggaca acgcattttg ctgataacgc agcagttcgc ctatgccttt
     1621 ttgcgccagt gcaaccagtt tgcccaactc gtccaaactg aatggcgcgc cttccgccgt
     1681 tccctgtatt tcgatgattt tccccgatgc ggtcatgacg atgttcacat cactgtcgca
     1741 gccggagtct tcgggataat ccaaatccaa aagcggcacg ccgttcacta cgcccgctga
     1801 tacggcggca acagcttcga ggatggggtt ttcactcaaa atgccgtctg aaaccagttt
     1861 gccgacggcg atttgcagcg cgacaaacgc accggtaatc gaagccgtac gcgtgccgcc
     1921 gtccgcctga atcacatcgc aatcaatcag gatttgtcgt tcgccgagtt tttccatatc
     1981 cacgaccgcg cgcagcgaac gcccgatcaa gcgttggatt tcttgtgtgc gtcccgactg
     2041 tttgcccgcc gaagcttcgc ggcgcatacg caaagcggtt gaggcaggca gcataccgta
     2101 ttccgccgtt acccagcctt ggtttttgcc gtgcagaaac ggtggggcat tttcgtccac
     2161 ggaagcggta caaatcactt tggtattacc gcattcaata aggcacgaac cgtccgtatg
     2221 cggcaggaaa tgaggggtga ttttgatatc gcgcaggctg tcggcggcgc gtgaggtgcg
     2281 gatataatca ggcatactgc cctcccgtta aaaacagata aattaaaaag ccttaaatat
     2341 gaaaaatcac atttaaggcc ttcaaactga aaatttctac gcctcttcgg ctttgctacg
     2401 gataatcaaa agcggcaggt ggctttggcg cattaccgtt tcggcgaaac tgcccatcaa
     2461 aaggtgcatc agcccggtgc gcccgtgcgt gcccaacacc agcaggccgg caccgttttc
     2521 atcggcataa tcgaccaaat cctgcgccat ttcgcgcgca cccttattgg caaccagcag
     2581 gtgtttgacg gtatttccca cacccagttc ccgggcggtg cgctcggcgg catccaaaac
     2641 ctcgttgcct tgcgcgacgg cggcggcctc gtaactttcg tgttgcaaaa attcgggggc
     2701 gagcgccata tattcggcag ggttggcaac gtgtaccaaa gtcagacgcg caccgttgac
     2761 cccggcaagc tcggcggcgt gtttcagggc attgatggac gtttcactgc cgtcaacggc
     2821 aacgaccaga tgtttgtaca tatcgtattc tccttttgca ccgtctcacg gtgccgtctc
     2881 ttcaggtttg cgcaaaggca gtctgcgccg tttcattata gacccgccgt cgggctttat
     2941 acaacagccg aacagcctga ctgctttcca gtataatatg ccgctttcgt gcagtcaggc
     3001 attttttttg ccggctttcg ttcacttttt gatttgacgc aatcttgcag gattcgacca
     3061 tgtccgacaa cgctttgacc tcttcgcgac gcttcggcgg catcgccaga ctctacggag
     3121 actctgcctt gacgcacttt tcacaggcac acgtctgcgt ggtcggcgtg ggcggggtcg
     3181 gctcgtgggc ggtcgaggct ttggcgcgca cgggcatcgg gcgtttgact ttgattgatt
     3241 tggacaacgt tgccgaatcg aatgtcaacc gccagctgca cgccctgacc ggcgacttcg
     3301 gcaaagcaaa agttaccgcc ttgcgcgaac gtattacaca aatcaatccg caatgcgaag
     3361 tatttgaaat tgaagatttc gttaccgaag acaatttgcc ggaatacttc ggaaaaggtt
     3421 ttgatttcgt catcgacgcg atcgaccaag tgcgcgtcaa agcggcaatg gcggcttatt
     3481 ttgtggaacg caaacaaccg tttgtcctca gcggcggcgc gggcggacag aaaaatcccg
     3541 ccctgattca aaccgccgat ttaagccgcg taacccacga cccgctgctt gccaacctgc
     3601 gctacacctt gcggaaacgc tacggcttca gccgcgatac taaggaaaaa atgcgcgtgc
     3661 cgtgcgtgta ttcgaccgaa aatatcatgc cgccgcaatc gggcgcagcg tgttctgccg
     3721 atgccgcgcc gcagggcttg tcatgcgccg gctatggcgc aagtatgctc gttaccgctt
     3781 cgttcggact gtattgcgca caggcggcgg tggaacacat cgcgggcaaa aaataagcaa
     3841 tgccgtctga aacaggattc agacggcatt tgaacaaacg cggcaatcaa gctgtcggtt
     3901 cagaaaaacc aacatcggca cgcctaaaac cgtattcaca atctacccct tttcaaaata
     3961 tttgcattta aaagccgtta taatgccgtc cgaacatctg cccgaccaca ttatgcgtga
     4021 atgccggcag attgttttct tttgtaaact tatattaaaa ttcacttacc aattcacgcc
     4081 atgccgccca tccctgccct atccgcacca tccgagcaca ctgtcgcatg ggtattcggc
     4141 caacccgtta ccgatttgcc gcaggatttg tttatcccgc ccgacgcgct gaaagtcgta
     4201 ttgggcagtt ttcaaggccc tttggatctg ctgctctacc ttatccgcaa gcagaacatc
     4261 gatgttctcg atattccgat ggtggaaatt accgggcagt atctgcacta tattgcccaa
     4321 atggaagcct atcagtttga tttggcggcg gaatatcttt tgatggcggc aatgctgatt
     4381 gaaatcaaat cgcgcctgct gctgccgcgt accgaagccg tcgaagacga agaggccgac
     4441 ccgcgtgccg agttggtgcg ccgtctgctt gcctacgagc aaatgaaact ggcggcgcag
     4501 ggtttggacg cgctgccgcg tgcgggacgg gatttcgcgt gggcttacct gccgctggaa
     4561 attgcagccg agacgaagct gcccgaggtt tacatcgccg atttgatgca ggcatggttg
     4621 ggcattcttt ctcgggcaaa acatacgcgc agccacgaag taatccaaga aaccctttcc
     4681 gtgcgcgcgc aaatgacggc aatcctgcgc cgtttgaacg aacacgggat atgcaggttt
     4741 cacgacctgt tcaatcccga acagggcgcg gcttacgtga tcgtcaactt catcgccctg
     4801 ttggagcttg ccaaagaagg attggtcgga atcgtacagg aagacggttt cggagaaatc
     4861 cgaatcagcc tcaatcatga gggggcgcat tcagacggca ttttcggcac acggggcggg
     4921 cgcgatgtgt tctaatacgc cccaagccgc caccagaaat cgggagacac gccatatgac
     4981 cggcatcata cattcgctgc tcgacactga cctctacaaa ttcactatgc tgcaagtagt
     5041 cctgcaccag tttccgcaga cgcacagcct ttacgaattc cgctgccgca acgtctcgac
     5101 cgtctatccg cttgccgaca tcagggagga cttggaagcc gaactcgacg cgctctgccg
     5161 gctgcgcttc acccacgacg aactcggcta tctgcgctcc ctgcgtttca tcaaaagcga
     5221 ctttgtcgat tatctcgaac tcttccagct ccaacgccgc tttgtcgaag tcggcacaga
     5281 cgataaaggc cgtctgaaca tccgtatcga aggcccgatg atacaggcga tgttttttga
     5341 aatcttcatc ctcgccattg tcaacgaact ttacttccgc cggctggaaa cccctgccgt
     5401 tatcgaagaa ggcgaacgcc ggcttcaagc caaagccgcg cgcctgaaag aaatcgccgc
     5461 cgcacagaat ccggacgaac cgcccttcct gatttccgac ttcggcacgc gccgccgcta
     5521 caagctcgcg tggcaggaac acgtcatccg caccctgctc gaagccgccc caagcatcgt
     5581 gcgcggcaca agcaacgttt acctcgccaa aaaactcggc atcaccccca tcggcaccat
     5641 ggcgcacgaa ttcctccaag ccttccaagc cctcgacgtg cgcctgcgga atttccaaaa
     5701 ggccgcgctc gaaagctggg tgcacgaata ccggggcgat ttgggcgttg ccctgaccga
     5761 cgtggtcggt atggatgcct tcctgcgcga tttcgacctc tatttcgcca aactcttcga
     5821 cgggctgcgc cacgacagcg gcgaccctta cgtttggggc gacaaagcct acgcccacta
     5881 tcaaaagctc aaaatcgaca gccgcaccaa aatgctgacc ttctccgacg ggctggacat
     5941 cgaacgctct tgggcattgc accaatattt caaaggccgc ttcaaaaccg gcttcggcat
     6001 cggcaccaac ctcaccaacg atatggggca tacgcccttg aatatcgtgt tgaaactggt
     6061 cgaatgcaac gggcagtccg tcgccaagct ctccgactcg ccgggcaaaa ccatgaccaa
     6121 caacagcacc ttcctcgcct acctgcgcca agtgttcggc atacccgaac ctagaacgcc
     6181 gtaaaccggc agaaaaagcg tacaattcct gtttccgccg cataaaatct tttaaaatac
     6241 cgcctgattt gaatttaacc gaaagaccga acttcatgaa cctacatcaa accgtcgaac
     6301 acgaagccgc cgccgccttt gccgccgcag gcatcgccgg cagccccgtt gtcttgcagc
     6361 cgaccaaaaa tgccgaacac ggcgatttcc aaatcaacgg cgtgatgggt gcggcgaaaa
     6421 aagccaaaca aaatccgcgc gaactggcgc aaaaggtcgc cgacgcattg gcgggcaacg
     6481 ccgtcatcga aagcgcggaa gtggccggac ccggctttat caacctgcgc ctgcgccacg
     6541 aatttctcgc tcaaaacatt catgcggctt tgaacgacgc gcgtttcggc gtagcgaaaa
     6601 ctgcccaacc gcaaaccgtc gttatcgact attcttcgcc taatctggcg aaggaaatgc
     6661 acgtcggcca tctgcgctcc agcatcattg gcgacagcat ttcacgcgtg ttggaattta
     6721 cgggcaacac cgtcatccgc caaaaccatg tcggcgactg gggtacgcag ttcggcatgt
     6781 tggttgctta tttggtcgag cagcagaaag acaatgccgc gttcgagctg gcggatttgg
     6841 agcagtttta ccgcgccgcc aaagtgcgct ttgacgaaga ccctgccttt gccgacaccg
     6901 cacgcgaata cgttgtgaag ctgcaaggcg gcgatgaaac cgtgttggcg ttgtggaaac
     6961 agtttgtcga tatttcgctc tcgcacgccc aagccgttta cgacacgctg ggcttgaagc
     7021 tgcgccccga agatgtggcg ggcgaatcga aatacaacga cgatttgcag cccgtagccg
     7081 atgatttggt tcaaaaaggc ctggcggttg aggacgacgg cgcgaaagtc gtgttcttgg
     7141 acgagttcaa aaacaaagag ggcgaacctg ccgcatttat cgtgcaaaaa caaggcggcg
     7201 gcttccttta tgcttccacc gatttggcgt gcctgcgcta ccgcataggc cgtctgaaag
     7261 ccggccgcct gctgtacgtc gtcgaccacc gccaagccct gcacttcgag caactcttta
     7321 ccacttcccg caaagcaggc tatctgcctg aaaatgcaaa agccgagttt atcggcttcg
     7381 gcaccatgat gggcaaagac ggcaaaccgt tcaaaacgcg cagcggcgac accgtaaaac
     7441 tggttgacct gctgaccgaa gccgtcgagc gcgccaccgc tttggtgaaa gaaaaaaatc
     7501 ccgaattggg cgcggatgaa gccgccaaaa tcggcaagac cgtcggcatc ggcgcagtca
     7561 aatacgccga tttaagcaaa aaccgcacca gcgattatgt gttcgactgg gacgccatgc
     7621 tctcatttga aggcaacacc gccccctacc tgcaatacgc ctacacccgc gtgcaaagcg
     7681 tgttccgcaa agccggagaa tgggacgcaa ccgcaccgac cgttttgacc gaaccactgg
     7741 aaaaacagct tgccgccgag cttctgaaat ttgaaaacgt gctgcaaagc gtggcggaca
     7801 ccgcgtatcc gcactacctc gccgcctacc tttaccaagc cgccaccctg ttcagccgct
     7861 tctacgaagc ttgcccgatt ttgaaagccg aaggcgcaag ccgcaacagc cgcctgcaac
     7921 tggcaaaact taccggcaac acgctgaaac aaggcttgga tttgctgggc atcgatgtgt
     7981 tggacgtaat gtaaacaccg caccgcccgc catcctcatc cgaatctgaa aaaagcggcg
     8041 cgatacaccg tatccgccgc ctttcccaaa atgcgaaaca aacaaacgcc aagcaagcaa
     8101 gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aaaaattata
     8161 gcccctttcc tgccggcgca cacactttcc gcgcggcaca tccccctttt tccgcccctc
     8221 aaacccgcct tttcttcagg cagggtttca gcccgcctcc tttccctgtt ttcctttccc
     8281 cgacacgcgt gcgcgcccct gccgcaccgt gccgcgcttt tgcgcccgga cggcatcgtt
     8341 ccgccatctg gttttctgtt ttaaataccg ctgtttcaga aagaaatgca gatgtttcga
     8401 cacacaggac gacatataaa gtaccgccct atgtgtcgtc ctgatttgga aggggttaca
     8461 cccctcccaa ccagataaag tctgatcctg ccgccccgaa ggacggatgt ccaagtggcg
     8521 gggtttcaac cgaaaaggaa atacgatgaa tattcacgcc ctgctctcca aacaatggac
     8581 gctgccgcca ttcctgccga aacggctgct gctgtccctg ctgatactgc tggtccccaa
     8641 tgcggtgttt tgggttttgg cactgctgac cgccaccgcc cgcccgattg tcaatttgga
     8701 ctaccttccc gccgcgctgc tgatcgccct gccttggcgt ttcgtcaaaa ttgccggcgt
     8761 attggcgttt tggccggcgg ttttgtttga cgggctgatg atggtgatcc aactcttccc
     8821 ttttatggac ctcatcggcg ccatcaacct cgtccccttc atcctgaccg cccccgcccc
     8881 ttatcagata atgaccgggc tgttgctgct gtatatgctg gcgatgccgt ttgtgttgca
     8941 aaaagccgcc gtcaaaaccg acttccgaca cattgccgtc tgtgccgccg ttgtggcggc
     9001 agccggctat ttcaccggcc atttgagtta ctacgaccgg gggcggatgg ccaatatctt
     9061 cggcgcaaac aacttctatt acgccaaaag tcaggcgatg ctctacaccg tcagccagaa
     9121 tgccgacttt attaccgccg gcctggtcga ccccgtcttc ctccccttgg gcaatcagca
     9181 gcgtgccgcc acgcggctga gtgagccgaa atctcaaaaa atcctcttta tcgtcgccga
     9241 atcttggggg ctgccgggca atcccgagct tcaaaacgcc acttttgcca aactgctggc
     9301 gcaaaaagac cgtttttcgg tttgggaaag cggcagtttt cccttcatcg gcgcgacggt
     9361 cgaaggcgaa atgcgcgaat tgtgcgccta cggcggtttg cgcgggttcg cactgcgccg
     9421 cgcacccgac gaaaaatttg cccgctgcct ccccaaccgt ttgaaacaag aaggttacgc
     9481 cacctttgcg atgcacggcg cgggtagttc gctttacgac cgcttcagct ggtatccgag
     9541 ggcgggcttt caaaaaatca aaaccgccga aaacctgatc ggtaaaaaaa cctgcgccat
     9601 tttcggcggc gtgtgcgaca gcgagctgtt cggcgaagtg tcggcatttt tcaaaaaaca
     9661 cgacaaggga ctgttttact ggatgacgct gaccagccac gccgactatc ccgaatccga
     9721 cattttcaac cacaggctca aatgcaccga atacggcctg cccgccgaaa ccgacctctg
     9781 ccgcaatttc agcctgcaca cccaattctt cgaccaactg gcggatttga tccgacgccc
     9841 cgaaatgaaa ggcacggaag tcatcatcgt cggcgaccat ccgccgcccg tcggcaacct
     9901 caatgaaacc ttccgctacc tcaaacaggg acacgtcgcc tggctgcact tcaaaatcaa
     9961 ataacaacaa tgccgtctga acgcaccaac agccttcaga cggcattttg cagacagacc
    10021 gacctcctca agcccacttt tttcatcatc tccgataaat tgctttgtaa gaatccaaaa
    10081 atccaaaatc acaggaagtt atcaaaaaaa cagaaaccat ccgccgtcgt tcccgcgaaa
    10141 gcgggaatcc agacccggaa accgcagaaa tttatcggaa aaaccgaaac tcgaaaacaa
    10201 cggacagaat tcccgcctgc gcaggaatga cggctcatac atcacccaaa aaaaattgaa
    10261 accaaacagc tgggattccc tcttgcgtga ggctgacaga tgccgtctga aagactttca
    10321 gacggcatag ggcgttttct ctttgaattt aaattttgcc acaaaaaagc cgccccaaat
    10381 gaatacccgg gcagcttttt gttgatatga ctccaatcag cggtgttgcg gattgtaacg
    10441 tttttccaaa cgcaggaata tccagcctaa gaaagtcgtc atcaacagat aaatcagggc
    10501 ggcggtgtaa agcggttctt cataaaccga ataccggccc gtaatcgtat tctgaacata
    10561 cgccaactcc gccacagcaa tgaccgacag cagcgagctg tctttcaaga gcgtgatgaa
    10621 ctcgctcgcc aaaggcggca gcatacggcg caatgcctgc ggcagaatca catagcgcat
    10681 cgcctgcgga taggtcagtc ccaaagaaca cgccgcctcc atctgtcctt tgtctataga
    10741 ctggatgccc gcgcggaaaa tctcacagat atacgccccc gagttggcga tcagtgccaa
    10801 agaaccggca atcagcggcc cgtatccgcg acgcagcgcg attgccgcct cgccgctgac
    10861 caaaatgccg tctgaaggat ggacgaaaaa cggaaaccac acatacgccc aaatcacaat
    10921 ctgcacaaac agcggcgtac cccggaacag ggtaacgtac agcagcgaaa ccttacgcaa
    10981 cgcccacgcc agcacgcgca tcggcgcacc ggctttttcc aagtgaatca ggcgcgccaa
    11041 cgccaacaac agacccaata ccgaaccgcc cgccgtcgcc acgaccgtca gccccaaggt
    11101 cgtcagtgcg ccgtaaagaa acatccagcg gtattcgtaa ataatgtcaa aacgaaaatc
    11161 cataaaccgt ccgtatcaaa aaccggcgga actgccgccg ttgcaaaata atccgccatt
    11221 ttaccgtaaa aaccgccgcc tgaacttttt tatcgcggca gacggcggtt gcgcgtctcc
    11281 gcaaaaatgc agggcgcgcg gttttcagac ggcatttgcc gttcaaggcc gtgcggtgtc
    11341 tttaccaaat gcccaaccat tcgcccacgg aatccatcca atccttattg cccccgccgc
    11401 tcctgcctgc ccggcggtac gcccacggcg cttgcggatt tttagctttc cacaatcctt
    11461 tgcgttccct ttccgcctga atttgagcgt cggcatagtc ggcaaaatcc gccttatcct
    11521 gctgttcttt agcataactt ttataatgcc acgccgcccc gtcctgcacc tgcatcaggt
    11581 tcaaatcggt tttgccggcg gatacctgcg ccacttcgcg ctgatagcgg tcggtttcaa
    11641 acacacgtac actgactttc ctaccctccg ccgccgcgcg caggttgtcg cgcgaacgtg
    11701 taccgtaagc ctgtttcatc tccggtgcgt cgatatacgc catccgaatt ttatgtttcg
    11761 cgccgtcgcc gtcgatgacg tgaagggtat cgccgtcata gactttggac accgtgcctg
    11821 tgtagctgtg gccggatttc gccgatgccc gtcggcgaac gggcgcgtcg gaacccacgt
    11881 cccctgccgt gccgagtacg tcgagtacgg caaccgccgt ccgcaccgcc tcactgtcat
    11941 atcccgtata acccaacgcg cccaaaagcg acagggcgac gggaagccat ttcatgattt
    12001 ttttaatctg catatttttc aaatgccgat gccgtctgaa catctctgaa ccggatttca
    12061 gacggcatct taacgtcagg attacccttg gcagggatag atgactttcg caccctcttc
    12121 cgtccccaaa atcaacacat cggcggcatc gcgggcgaat atgccgtttt cgagtacgcc
    12181 ggtgattttg ttgatttcat cttccatcgt cagcggctga tcgatattca agccgtggac
    12241 atcgacgatt tggttgccgt aaaacgtggt gtagccgata cgcagttcgg gctgtccgcc
    12301 catggcaagc agtttgcgcg aaacaaggga gcgcgcgctt tcgacggctt ccacgggcag
    12361 tgggaatttg cccagccggg aaacatattt gctttcgtcc gcaatgcaga cgaatttttc
    12421 tgatgcgctg gcgacgattt tttcgttgag gtgcgcgccg ccgccgccct taatcatttg
    12481 cagggcgtgg ttcacttcgt ccgcaccgtc gatatagacc gccaaaccgg acacttcgtt
    12541 cagggaaacg acgggaatgt cgtaccgggc aagcagttcg ccggattttt tggaggttga
    12601 aaccgcgcct ttgatttttt taccgctttt gcccaaggct tcgatgaaga aattgatggt
    12661 cgaaccggta ccgatgccga tgtattcgtt ttcgggtacg aattcgactg ctttttcggc
    12721 ggcgatgcgc ttgagttcgt cttgtgtcgt catattttta tcctttggga aaccgtatca
    12781 acaaacagcc gccatcttaa catttttttg cacgtcctgc ccgccgcgtt caaatccgta
    12841 ccagcaatac cgccgcctgc gcctctatgc cttccatccg cccgagatag ccgagttttt
    12901 cgttggtttt gcctttgata ttgacgcacg aaatatcgat acccaaatcg gcggcgatgt
    12961 tggcacgcat ttgcggaatg tgcggtgcga gtttgggttt ctgtgcaatc acggtcatat
    13021 cgacattgac cgcctgccaa ccctgcgcct gaacgctttg atacgccgca cgcaaaagga
    13081 cgcggctgtc cgcatctttg aactctgcgg cggtgtcggg aaaatggctg ccgatgtcgc
    13141 ccaaacctgc cgcaccgagc agcgcgtcgg taacggcgtg cagcagcgcg tcggcatcgg
    13201 aatgcccgag cagccctttt tcaaatggga tttcaactcc gccaagtatc agctttctgc
    13261 cttcggtcag ttggtggaca tcgtagccct gtccgatacg gatattcgtc atcgtttgtg
    13321 ttcctgatgt tttgaattga atttcagacg gcattgagca gcagcctgac gatgtatgcg
    13381 tcctgcggct gcgtcagttt caaattgcgc gcgtcgccct gtatcagtag cggacgcaca
    13441 cccagttttt ccacggcgga cgcttcatcg gtaatgccgc ccaagttttc cgcagccaat
    13501 gcgcggtgca gcaaacccgc ttgaaaaagc tgcggcgttt gcgcctgcca aagccccgaa
    13561 cggtcgacag ttgcactgat ttgtccgctt tctgcgcgct tgatcgtatc ggcaacggga
    13621 actgccaaaa tcccgccttc ggcggcgttg cccgcctgtt ctatcaaccg cgtcagagct
    13681 tcagacggca ggcagcagcg ggcggcatcg tgtaccagaa tattgtcggt ttccgccgcc
    13741 aaaccggttt ccaacagttt tgccacaccg ttgcggacag tttcggcgcg ggtctgtcca
    13801 ccgtttttcc acacccgaac ctgtggaaat gccgtctgaa ccttatcggc aaacgtgtct
    13861 tcgggcgaga cgacaacgac ggtcaaatcg acggcctcat gccgttcaaa aatcccaagt
    13921 acatgttcta aaacggtttt gcttccgatt tcgacatatt gcttgggttt gtccgcaccg
    13981 aaacgcaccc cgatgccggc ggcgggaatc agcgcgatat ttttgcgttt catgcgtttt
    14041 ccccgccgtt ttcagacggc acggcttcct tacgccaaat acaggcttcg cccaagccgt
    14101 cgagatattc ttcgtgcgcc gccagttcgg cttcatccgc cttaatcact ttcagtttgg
    14161 caccgagttt catttcggca gatgcaacag gtttggctgt cggttcttcc tcctcggtct
    14221 cccccatcaa gtcgaattgc cgccgcgtca tcgccagata gacttcgccc aaaagttcgc
    14281 agtcgatcaa tgcgccgtgc aggacgcgct tgctgcggtc gacggaaaaa cggttgcaca
    14341 gggcatccag actggctttc tgcccgggga acatttcgcg cgccatcgcc agggtatcgg
    14401 taacggtgca gccgagttcc tcgacggtcg gcagccccat ccggcggaac tccatattga
    14461 ggaagcccac atcgaatttg gcattgtgga taatcagttc cgcaccgcgc agaaaatcgg
    14521 caatctgcct gccgacctct gcaaacggcg gcgcgttttt ctcttccaaa acctgtatcg
    14581 tcaagccgtg gacgcgtgcc gcctcttcgg gcatatcgcg ctcggggtgg acatagaggt
    14641 gcaggttttt gtcggtcatt tggcggttga ccatttccag accggcaaac tcgaccagac
    14701 ggtcgccgcc gtcggcatac aggccggtgg tttcggtatc gaggatgatt tggcgtgtca
    14761 tcatatcggt gtctttcttc tgtcttcgta aattgattat tttttaagca atgtattttt
    14821 ctgttttcat ttcaatgcac aaacccactt attcacagtg tgttcacaac attgggcagg
    14881 cggattgtgt attttgggga caatttttcc agacggcatt caaggttttt tcctgattgc
    14941 cgccgcgcct aaaaaccgcc tttcgcgctt aatcaaaaat accgccaacg gaatattgcc
    15001 caaggcgaca atcagataca gcagggaaat gctgtcaaac aaaaacagca ataccgcgct
    15061 caaaacggcg gcggaaacca taaagatgcc gttaacgata ttgttggcgg caacggcgcg
    15121 ggcgcggaaa gtctcgctgc tggcggtttg cagccaggta tagagcggaa cggagaaaaa
    15181 tccgccgaaa aagccgatca gcgtcatcac cgccatcacg gggtatgccc atccttgcga
    15241 taaaaaccaa aaaatgccgt tcagcccttc aaaacggtgt ccgtgcgtca gccacaccaa
    15301 aaccaggccg caaaccgtca aacccaacgc accaaccgtt acccaagcca acatcagccg
    15361 ttccctgccg aacttggcac acagtaccga accggcggca ataccgatgg aaaacaaagc
    15421 aagcatcagg ttaaaaacat tatcgttgcc gcccaaacgg atttgggtaa aggtcggcag
    15481 ttgcgtggta taaaccgcgc cgacaaacca aaaccacgag atgccgataa tggcggtaaa
    15541 aacgggattg tgccgcaccg tttcacgcag cagggatttt gtaccacgga caatattcca
    15601 ctcgatttgg gtatcggcag ccttggcggg tacggacggc ataaacaggc tgccggccgt
    15661 tcctccgacg gcgaccagca aaaccagtat cccgacaata taaggcggcg cgccggcaac
    15721 cgccgttccc aaaatctgac caaacaggat ggcgacaaac gtaccggatt caatcaggct
    15781 gttgcccatc atcagctcgt tgtcgtcgag ataatcgggc agaatggcat atttaagcgg
    15841 gccgaacagc gtcgattgcg cgcccatgca aaacaggcag agcaagagca gcggggctga
    15901 ccggatataa aacccgtatg ccgccaccgc cataatgatg atttccagca ccttgaccca
    15961 acgcgccaaa acggccttgt cgaatttgtt acccaactgc cccgacagcg cggaaaacag
    16021 gaaatacggc aaaataaaca gcagtgcgcc caagttcaac atctgtccgg caggcagaac
    16081 gccgttttgc cccaaaccgt aaaacccgat catcacaaac agcgcggttt tgaacacatt
    16141 gtcattgaac gcgccgagaa actgcgtagc gaaaagaggc ccgaaacggc ggcttttaac
    16201 cagtcccaaa ccgccttttt tagcgtccat cgttttcctt ctctttttca atcagtttac
    16261 ttgtcgaatc atcatccatc aaaatgcggt gcgccggtcc ttccaaatcg tcaaactgtc
    16321 cgtttttgcc cgaccaccag aaaaaccagc cgatgacaaa tgccaaaata atgctgatgg
    16381 gcaccagaat aaacatactt tccatcacat attccctatc aaatcgttca aaacaaaagt
    16441 ctgccctgac acggtcagat attcgttacg caaagttccg acgggggctt cgtcaaaaaa
    16501 cagctcgata cggtctttga ccacgcgcca atattgggga atttccgtct gaccgaacgg
    16561 cgacaggaca tggttgcccg tcccatcttc caatttaacg gcaaaacgcc cgctctgcag
    16621 ccgtgcttcc gattcgtcgt cggcaagaag gatgaaaaag cctatatgcc gtcccgattg
    16681 gtcatgaata ctgaaataat gcataaattc cccacccgcc ttttttcaga cggcatcaac
    16741 taaaaacagg gcgaatgtac cagtttggac gggaaaaatg caaagaaatt ctccctcccc
    16801 cagccgaaaa caccggcaaa ccgcatatcc cccttttttc cgtcaaaata cccgacttcc
    16861 gccattttca cgcaaacgcc cgattaagcc aagcaattgc aaagattttt tgctagaata
    16921 gcctgcttct tttatcaacc gtttcagacg gctccactac tttcccgtca aggaaagcaa
    16981 agcggattcg gcacgaatcc gattagtatc cgtgtccgat tccaatgccg tctgaaactt
    17041 tccggagtaa gaaaatgtcc caaaaattga tcttggtttt gaactgcggc agctcgtccc
    17101 tcaaaggcgc ggtcctggat aacggcagcg gcgaagtcct gctgagctgc cttgccgaaa
    17161 aactcaacct gcccgatgcc tacattacat tcaaagtaaa cggcgaaaaa cacaaagtcg
    17221 atctgtccgc acaccccgac cacaccggcg cggtcgaagc cctgatggaa gaactcgaag
    17281 cccacggcct cgacagccgc atcggcgcca tcggccaccg cgtcgtcagc ggcggcgaac
    17341 tgtacaacga atccatcctc gttgacgacg aagtcatcgc cggcatcgaa aaatgcatcc
    17401 cgctggctcc tctgcacaat cccgcccacc tcttgggcct gcgtgccgcg caaagcattt
    17461 tcaaaggcct gcccaacgtc gtcgtattcg atacctcctt ccaccaaacc atgcccgaag
    17521 tcgcctacaa atacgccgtt ccgcaggagt tgtatgaaaa atacggcctg cgccgctacg
    17581 gcgcgcacgg taccagctac cgcttcgttg ccgacgaaac cgcgcacttc ctcggcaaag
    17641 acaaaaaaga cctgcgtatg gtcattgccc acttgggcaa cggcgcatcc atcaccgccg
    17701 tcgccaacgg agaatcgcgc gacaccagta tgggcctgac cccgctggaa gggctggtaa
    17761 tgggtacgcg cagcggcgac atcgatcctt ccgtattcgg cttcctcgcc gaaaacgcca
    17821 atatgaccat tgcccaaatc accgacatgc tgaacaaaaa atccggcctg ctcggcattt
    17881 ccggcctgtc caacgactgc cgcaccattg aagaagaagc cgccaagggg cataaaggcg
    17941 cgaaattggc cttggatatg tttatctacc gccttgccaa atacatcggc agtatggcgg
    18001 ttgccgcagg cggcttggac gcactggtct ttaccggcgg tatcggcgaa aactccgaca
    18061 tcatccgcga acgcgtaatc ggctacttgg gcttcctcgg tctgaacatc gaccaagaag
    18121 ccaacctgaa agcccgcttc ggcaacgccg gcgtgattac cactgccgac agcaaagccg
    18181 ttgccgtggt cattccgacc aacgaagagc tgatgattgc ccacgatacc gcccgtttga
    18241 gcggtctgta aggttttatc ctcacgcgaa ctgcctccgg aaacggaggc agttttttta
    18301 tagtggatta aatttaaacc ggtacagcgt tgcctcgcct tgccgtacta tttgtactct
    18361 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatccggc tttccatgct
    18421 taaacagcac tacctctttt cagacattga cggttgcagc cgcctacctg aacattaatg
    18481 attgactatt ttttaatcat gttattattt tccataaaat acatggcatt aagatgtttt
    18541 tccacaaaag atacacacac cggcaaacac cggctgtgtt tatcttttct tatgcctatt
    18601 ttttaatcat cgtattttta tcttttaatt tcaatacgca aaccgactta tacacacggt
    18661 tttcacatct ttggactgct tccgtgtgta tagcggatat tgccgttttc ctgtctgaca
    18721 aaaatgccgt ctgagaactt cagacggcat ttgaaacatc ggaatcagcg gttttgttca
    18781 taccactcga taaacttgtc tgctttgaca aaacccagca gcggctcgct gcggctgccg
    18841 tcggcgcgga cgacaaacac gcccggcggc ccgaacagac cgtattcttt caacaacgcc
    18901 tgatgttcgg gcgtgttggc ggttacgtcg atctggaaaa agcgttccat atcgactgcc
    18961 tgatgcactt ccggctgatt gagcgtgtaa accgccattt ctttgcagga aatgcaccag
    19021 tcggcataaa aatccaaaac gacaggtttg tcgggatgtt ctttcaacgc cgtatccatc
    19081 gctgccttca gcgcggcagt atcggcaaac attttgccgt gttccgaaaa tttgcctgct
    19141 tcggccggcg gattgagggt caagaaatgg tgcagcgcgg tcgttttgcc gtttgcgccc
    19201 tgccagccga accacgcgcc gcctatcagc aatatgctgc ccaatgcgaa tgccacagct
    19261 ttcggacggc gtttctgcct gcgtccgttg accagcagca taaaggcagg aaccagcatc
    19321 agcagcgtgt acagcgcgac gacgagataa tagggcaaat gcggcgtggc gaggtaaacg
    19381 gcgacggcaa gcaggataaa gccgaaagcg tatttgacgg cattcatcca atcgcccgcc
    19441 ttaggcagga tatgtcctcc gaacgtgccg atggcaatca gcggcacgcc ggtgcccaac
    19501 gccaaagtgt aaagtgccaa accgcccaaa accgcatcgc ccgtctgacc gatatagccc
    19561 aaggcaaacg ccagcggcgg ggcgacgcac ggtccgacaa tcagtgcgga cagaatgccc
    19621 ataataaaga cggaaacgat tttaccgccg gagagcctgc tgctctggtt ttggaaatat
    19681 gactgtacgg cgttgggaag ctggatgttg aacagcccga acatagacag cgccaagacg
    19741 accatcaaag ccgatgccgc caacacgacc caagcctgct gcaaccatac ggtcagcagc
    19801 gcgcccgtca gtccggcaac aatgccgacc agcgtataag tcagagccaa accctgaaca
    19861 taaacgacgg acagcacaaa cgcccgcgcc ttgcccgcct ttttgtcacc gaccacgata
    19921 ctggacacaa tcggcagcag gggatacata caggcggtaa aactcaggcc caaaccggcg
    19981 agaaaaaacg ccaaaagatt ggcgttgagc gtatcccaag acagcttgaa acggccgtcg
    20041 ccgccctcgt cccccttcgg gggcggcaac gccccgctgc cgttttgaga ggaaggctgc
    20101 aaaaaacggt ctttggcgga tgccggttcg tcggtttgcg gatggtaagt gccgttgccg
    20161 gaaatatcaa actcggtatc cacgggcgga tagcacacgc cggcttcggc acagccctga
    20221 taggtcaaaa ccaatttata cggttcgccg acagcctttg cataaggaaa ggcaacctgc
    20281 gcctcgtgat ggtgaaccgt ctgcctgccg aaaaactcgt cttccttctc ttcgccctta
    20341 ctgaaagaag gctgtcccaa caaatccgcc ggatcggtct tgccgacgat tttcgcctga
    20401 tacatatagt atccgtcggc aatcctgaaa cggacgttca cgccgtcgtc ggcaacagta
    20461 agctccggca cgaatgcctt ttccggcggc agcagatcgt tcgcatccag cgcgaaagcc
    20521 cgtccgcaca acatcaaaaa tacggcgaac aggcaaatca gttttttcat aatcgaatcc
    20581 gtttcagaca aataatttgt ccgcattata aatggtaagg ttgacggtag gatttaattt
    20641 atgtaaaacc cgccattatc cgaacctatt tccataaaca tcttatcgga cccgccatgt
    20701 acgatgtcaa tacccacgat gtccgccgct ttttcgcccg cgtgtggcag cagcggctca
    20761 atccgctgca attgggcgcg ttggaacaga aagccctccg cattgtcgaa gcccatcccg
    20821 aataccaccg ttatctcgaa cgcatcgaag accatctgga caccgactgg ctgcccgaaa
    20881 acggcgaaag caaccccttc ctgcatatgt cgctgcattt gtccgtccaa gaacaggcgg
    20941 gcatagacca gccgcacggc atacgcgcca tccacgacac cctgtgcgcc aaacgcggct
    21001 ggccggaagc cgaacacgaa atgatggagg cactggcgga aacactgtgg acggcgcaac
    21061 gctacggcac cggtttggat gtcaatttct acatgacccg actgcgcaaa ctcatcggct
    21121 tgggtgcaga ggatcaagcc aggttaaacc cgcatgaaat cgcctgacca taccgaccgc
    21181 ctgcaaaatg ccgtctgaag cggaacaacc cctttcagac ggcattcatt ttcccccaat
    21241 catttccaca acgcgcgttt caacataatc aaccaatcct tcttatccaa aacggggcgt
    21301 tgtgcaaaca catcgtatcg gcacgcgtcc agtttctgca aaatcaactg cgcccccaac
    21361 acaatcatac ggagttccaa accgatacgc cctttcaatt cgcgcgccaa aggcgaaccc
    21421 gccttcagca tacggaatgc acgccggcac tcatacgcca tcagccgctg aaacgccgca
    21481 tccgcccgtc ctgctgcgat ctgttcctca gaaacaccga atttcaacaa atcgtcctgc
    21541 gggatataaa ccctgccctt ttgccaatcc acagctacat cctgccaaaa attcaccagt
    21601 tgcaaagccg tacaaatacc gtcgctttgc gctacgcaca ccgcatccgt tttcccgtat
    21661 aaagccagca taatgcgtcc gacagggttg gcggaacgcc ggcaataatc ggtcagatcg
    21721 ccgaaatgcg cgtaccgcgt tttaaccaca tcctgcgaaa acgccgagag cagatcataa
    21781 aacggctgca aatccaaacc gaacggcaca accgcctcgg catccaatcg tgcaatcaaa
    21841 ggatgcgccg accggccgcc cgatgccaac acgtccaact cgcgccgcaa accctccaac
    21901 cccgacaacc tggcttcaga cggcatactg ccctcgtccg ccatatcgtc cgccgtccgt
    21961 gcaaacgcat acaccgcatg aaccggcttc ctcaacctgc gcggcaaaac cagcgaaccg
    22021 acgggaaaat tctcataatg cccaaccgac ataccttctc catccatcaa acaaaatgcc
    22081 gtctgaaacg gaacaaaccc ttttcagacg gcatcagata cctccaagct gccggcaatc
    22141 agtggtggta atgaccgtgc gaaccgtgga catgaccgtg tgcgatttcc tcatcggatg
    22201 catcgcgcac gctttcaacc gtagctttaa agcggatttt catgcctgcc aaaggatggt
    22261 tgccgtccac caccgccttg ccgtcggcaa catcggttac acgatagacg acaacatcgc
    22321 cggtttcagg atcgtcggct tcaaacatca tgccgacttc gacttcaaca gggaacgcgc
    22381 ccgcatcttc aatacggacc aactccgaat cctgctcgcc gaacgcatcg tcggacgaaa
    22441 gcgccacatc gaccgtatcg ccggcatcct taccgtgcaa cgcctcttcc accaaaggga
    22501 aaatgccgtc gtaaccgccg tgcagatacg caatcggttc ttcggtttta tccaaaagct
    22561 gattgttggc atcatacatc tcataatgca gcgaaaccac ggaatttttt gcaatagcca
    22621 tatttgtcct ttcaggaaca gcagattgat tacaggcgca ttctaacaca accgccgcgc
    22681 cggccgatta ccgttaacct gttcataaac tgtacggcac atatttcaat gtaaattttt
    22741 gttattttat tgcagtgtaa attttttaca acactctcaa aaaccattcc gacccatctg
    22801 ccgacttccc caatctgcct taataaatca tacaagatac tgaattatat taatctctat
    22861 aatatttatc cctatcggat ttttaacagc aaaaccgttt tacaggattt atcaatccgc
    22921 ccgccagaaa acttttcatt caaacctttt tcccatctgt acgacattgc aatcccttat
    22981 tccatggtgc ataattacgc aaattcagcg atgaatttcc aacccggttt gtagcatggt
    23041 cgaaaaagac ctatttgttt caataattta aattgatttt aaaggttact aaaatgaaaa
    23101 aatccctgtt tgccgctgct ttgttgtctt tggctttggc tgcctgcggc ggagaaaaag
    23161 ccgctgaagc tcctgctgct gaggcttcct ctaccgaggc acctgctgcc ga
//
LOCUS       DAXFZR010000029        22795 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000029 DAXFZR010000000
VERSION     DAXFZR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22795
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.029"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(194..319)
                     /locus_tag="ACFJAS_000946"
     CDS             complement(194..319)
                     /locus_tag="ACFJAS_000946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982223.1"
                     /translation="MPPETDFAKRRRVTGVEMETERVKPDVSVLRKPAIKGLAGV"
     gene            359..595
                     /locus_tag="ACFJAS_000947"
     CDS             359..595
                     /locus_tag="ACFJAS_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC3982224.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            595..942
                     /locus_tag="ACFJAS_000948"
     CDS             595..942
                     /locus_tag="ACFJAS_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC3982225.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            complement(997..1149)
                     /locus_tag="ACFJAS_000949"
     CDS             complement(997..1149)
                     /locus_tag="ACFJAS_000949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982226.1"
                     /translation="MKFSINREFRKMLKLFAEYAVLRRMFYAVLGAAPLTALIWKPAD
                     ILNALK"
     gene            complement(1310..1642)
                     /locus_tag="ACFJAS_000950"
     CDS             complement(1310..1642)
                     /locus_tag="ACFJAS_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982227.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(1643..2113)
                     /locus_tag="ACFJAS_000951"
     CDS             complement(1643..2113)
                     /locus_tag="ACFJAS_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982228.1"
                     /translation="MLRVLLNRRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(2184..2489)
                     /locus_tag="ACFJAS_000952"
     CDS             complement(2184..2489)
                     /locus_tag="ACFJAS_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982229.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEHIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(2601..6746)
                     /locus_tag="ACFJAS_000953"
     CDS             complement(2601..6746)
                     /locus_tag="ACFJAS_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC3982230.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLIW
                     YGDFVTIEHKTTTRRGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRVWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVRMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLRQAFFTCQKPDEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKISGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNKVQAKLLLNGGVLA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRARGGGDYTEQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQVYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     QVG"
     gene            6986..7264
                     /locus_tag="ACFJAS_000954"
     CDS             6986..7264
                     /locus_tag="ACFJAS_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC3982231.1"
                     /translation="MESQTFASVFDALCDTPAEAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPA"
     gene            complement(7292..7723)
                     /locus_tag="ACFJAS_000955"
     CDS             complement(7292..7723)
                     /locus_tag="ACFJAS_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFC3982232.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(7725..8579)
                     /locus_tag="ACFJAS_000956"
     CDS             complement(7725..8579)
                     /locus_tag="ACFJAS_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFC3982233.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVKIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(8576..9175)
                     /locus_tag="ACFJAS_000957"
     CDS             complement(8576..9175)
                     /locus_tag="ACFJAS_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HFC3982234.1"
                     /translation="MGNAVFPEFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(9175..9441)
                     /locus_tag="ACFJAS_000958"
     CDS             complement(9175..9441)
                     /locus_tag="ACFJAS_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982235.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGGELSEDEFDALLKAKGII"
     gene            complement(9453..9782)
                     /locus_tag="ACFJAS_000959"
     CDS             complement(9453..9782)
                     /locus_tag="ACFJAS_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982236.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRREQEAAQA
                     GEARAAD"
     gene            complement(9843..10616)
                     /locus_tag="ACFJAS_000960"
     CDS             complement(9843..10616)
                     /locus_tag="ACFJAS_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982237.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDGDNYSYLQAAAPGGGKFAEDAGVMGQDGTAYIKVASSPKQGQYTVSESGVYAFN
                     SSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESVT
                     SGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(10642..11070)
                     /locus_tag="ACFJAS_000961"
     CDS             complement(10642..11070)
                     /locus_tag="ACFJAS_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982238.1"
                     /translation="MNREAVYSALWSKLDALGGFVTKSRKLLHWNDVKRYDQPALFMA
                     QGDMQALTLTGQETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(11067..11549)
                     /locus_tag="ACFJAS_000962"
     CDS             complement(11067..11549)
                     /locus_tag="ACFJAS_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFC3982239.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQSEVML
                     NRLSGQVLRVRTDNLRRSVHQRVNVSGNVVSGEVNTNVRYGIAHEYGFAGNVNVKASL
                     RQVRQAFGRPLKPPRYVHVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(11549..12079)
                     /locus_tag="ACFJAS_000963"
     CDS             complement(11549..12079)
                     /locus_tag="ACFJAS_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFC3982240.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(12082..12444)
                     /locus_tag="ACFJAS_000964"
     CDS             complement(12082..12444)
                     /locus_tag="ACFJAS_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982241.1"
                     /translation="MPSENQTAFSFGDFEMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAE
                     AAEPAEAEAEPAEAEKAE"
     gene            complement(12451..13950)
                     /locus_tag="ACFJAS_000965"
     CDS             complement(12451..13950)
                     /locus_tag="ACFJAS_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982242.1"
                     /translation="MNVNQLTQETIELMKSAQAGGGPPDKGFTQPAGFTAGLQTYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRTIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKISVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIEAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(13992..15149)
                     /locus_tag="ACFJAS_000966"
     CDS             complement(13992..15149)
                     /locus_tag="ACFJAS_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC3982243.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGRVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVELSESEIAALKAVLAKA
                     DKPKGGPAAKSMYQVKSPADVLMSLKWLVEDASYDNIDEAVTAQIKESAAGLAESLKA
                     LAASEADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
     gene            complement(15217..17364)
                     /locus_tag="ACFJAS_000967"
     CDS             complement(15217..17364)
                     /locus_tag="ACFJAS_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFC3982244.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPPAPAAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIEARKDQMECLKWTIQ
                     KRDVESTEDDESQRKDRKVDEAVAFFRSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGGLYALEVMDGATIKRVLDNTGRMPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQVHALEYYTAGSVPDALVGVPETWSADDIRRFQ
                     EYWDLLLSGETAQRRKMRFVPGELSRNFRETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMGSLKNWVKALIDDVLARYMDMAAYEFVWKGEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPGQGQPEPDKQDGRKPEEPPNQGAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIEGAAVDWQAGDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAGRLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEETGLVAGKQWITAKDDKVSDVCNANGGMGVIG
                     LHEPFSHGALTIPGHPNCRCAVVPVLAGDMPES"
     gene            complement(17361..18782)
                     /gene="terL"
                     /locus_tag="ACFJAS_000968"
     CDS             complement(17361..18782)
                     /gene="terL"
                     /locus_tag="ACFJAS_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC3982245.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGR
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQEDGTALWPEKHDIETLRRMEQAAPYVFAGQYLQR
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANGGDYTAGGRLGVTEDGRYII
                     ANVVRGRYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     GPESGDKVTRAGPFAAQVNIGNVMVLDDGTWDTDALIAEMRMFPNGRHDDQIDCLGRA
                     FGELLDTRTGMIDFLRSQVEAVK"
     gene            complement(18844..19293)
                     /locus_tag="ACFJAS_000969"
     CDS             complement(18844..19293)
                     /locus_tag="ACFJAS_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982246.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKTELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(19586..20455)
                     /locus_tag="ACFJAS_000970"
     CDS             complement(19586..20455)
                     /locus_tag="ACFJAS_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC3982247.1"
                     /translation="MNVIQSFNFNTTSPVRAFEKDGLTWFVAADVCKALEIQNPTQAL
                     EKLDDDERSMFNIGRSEIHGGGGNVNIINESGLYILILRSRKAMEQGSTAWKFRKWVT
                     SEVLPTIRKTGGYQVGQKTTADDRTGLRQAVAALVGRKGIDYSSAYSMIHQRFNVESV
                     EGIPAGKLPEAVAYVHALTLHTGLTGEVLDREPLPAPQPALPISGNALYDLAVAVSYG
                     ARAIQMGRDVSLPLKQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAEKI
                     RPILPEIRNLSAV"
     gene            complement(20736..21896)
                     /locus_tag="ACFJAS_000971"
     CDS             complement(20736..21896)
                     /locus_tag="ACFJAS_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC3982248.1"
                     /translation="MNAAAVSAVFKERIASHAEHVKKVAHICTTEETTKQALILPLLD
                     ILGFSAFDPNKVRAEYQADFPGAKSGERVDYALFCNGAPVMFIEAKSYTENLSNHCPQ
                     LSRYFNATPEIAICAITNGREWRFFTDLSNKNIMDSEPFLTVDVTMLNENDMAQLYQF
                     RHDKFQPDALRSLAEESIYLTAFTESITESLKEVDLDFVRYVAGRANIQRQFTQRYLE
                     SIRHIVKQAVQNTVSSMVVSGLSAPKVQEEAAPVEKEQEDPTAPIIDPENNKIVTTYS
                     ERRLFDLVKSILPDDASIEAKDTESYFGVLVDGKSNRWILRYFDNKQRPSVIFPIELE
                     ESDISNIERCGLEVSGNQVIIDTPENLLRVAWLVIDSYRFCCDDENFKRKPK"
     gene            complement(21987..22394)
                     /locus_tag="ACFJAS_000972"
     CDS             complement(21987..22394)
                     /locus_tag="ACFJAS_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982249.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GPLHLQTDLGLAAWYRAIRQAAGNDFDLLFEKV"
     gene            complement(22661..>22795)
                     /locus_tag="ACFJAS_000973"
     CDS             complement(22661..>22795)
                     /locus_tag="ACFJAS_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFC3982250.1"
                     /translation="VALDALQGVAYHNDRQVRRIAADYADEPVTGGGLAVEVGELDEK
                     "
BASE COUNT         4838 a         7057 c         5715 g         5185 t
ORIGIN      
        1 gtatctgttt ctttccacca cgctttctgc tcctgattaa aatttcccat ttcttgcagc
       61 atcgctaaaa gcacagggtt tgacttgcca ttcacttttt cgtgagcgcc aaggtattta
      121 cgggcttctt caatccattt caattctttc atttcttttc cttccgataa aagacgagat
      181 gtaaactttc ggtttacacc ccggccaagc ctttgattgc aggtttccgc aacaccgata
      241 catccggttt cacacgttcc gtttccatct ccactcctgt tacacgccgc cgcttggcaa
      301 aatccgtttc aggcggcata atgtatcgac aacgtatcta caacacgaaa gcaaaaccat
      361 gttgcgactg caaaaatggg gcaacagcgc ggcagtccgt ctgcccgccc gcctcctgac
      421 cgccctcaac gccaaaatcg gcgacgcgct ggaaacagaa atccgcaacg gcgagctgct
      481 catccgcccc gtcaaacgct accgcctgtc cgaccttttg gcggaaacgg aagccgcccc
      541 gccgcgcgcg gaaggctggg aagaaatgcc cgatgtcgga caggagggcg tttgatgtat
      601 attcccgagc gaggcgacat tttccatctt gccttcgacc ccgccgccgg tacggaaatg
      661 aaaggcggcc attacgccat agccctgtcg cccagagctt acaaccgcgc aaccggcttg
      721 gtttacgcct gcccgatttc acaaggcagg gcggcggcgg caagaagcgg cggtatgatt
      781 tccacactgc tcggcgcggg aacggcaaca caaggcaatg tccactgcca ccggatgaag
      841 gcattggatt ggaaaatccg ccgcgccgcc ttccgcgaaa ccgtccccga ttacgtgatt
      901 gaagacgtat tggcacgcat cggcgcagta ctgttcgact gacttcagag tgcgaagcct
      961 gtccgcaggg ggaagcatcc gtttccatac ccgcccctat ttcaaagcat tcaaaatatc
     1021 cgccggcttc caaatcaacg ccgtcaaagg cgcggcgccc aaaaccgcat aaaacatacg
     1081 gcgcaacacc gcatactcgg caaacagctt caacatcttg cgaaactccc tgtttatgct
     1141 aaacttcaaa cagatttctc cttacctcgg ataaggttaa attcagaaac gccgcaggga
     1201 ttccgcccct cgcggcgttt cgccgttttc aaacctgccg cgtaatgtcg tgcgtcagcc
     1261 ggacgcgccc ggaacacagc gtcttcacgc gccgcgatac cgctcgaaat caatcttttc
     1321 catccgaaga ccctccgtct ttcacacccg taagcttttc cgccaagccg cccagataac
     1381 cgcgcaaaac cttgggcgcg agcgcgcgca ccgtatcgag cgcgtgcccc gtcatcatcc
     1441 ccgcgaacac ccccgccgcc gcgcacgtcc acgcctgatc cagcggcacg aaccgctccg
     1501 ccgcggccgc cgccgcgatt gcagaaataa aagcctccag cacagcctgc accgggcggc
     1561 cgcgttcctg aaggcttgcc cacaccgcgc ccagcacgcc gccggccgcc gcaaacagca
     1621 gtccgaactg caaaaacccc attcacgacc cctcatccgc caaaccccgc gacttttgcg
     1681 ccgaaaacaa aaacctcaac gcattattcc ccgccagcgc gcagaaaaac gccaacagcg
     1741 gcggaacgac catacccgtg ttcaaaggcg ggtacgcccc ccaaaacgcc accgaaacca
     1801 aaaaccacac aaagcccgaa aacaaaagca ggtaggccga agccacacaa gccctcacgc
     1861 cccgcgcaaa cagcaatgcc gtctgaagca gccccgccgc cgccagcacc cccaccgtcc
     1921 acgcctccgg aatatcctgg aatttgtaat aaatccgcca cgcgtaaatc tcgtcgggcg
     1981 caagcgcgaa caccgccgcg taacccgaca agcccaaacc gctgaccgcc tccagcaccc
     2041 gcgtcgccgt gccgaacaac cacgcctgaa acccggcggg caaaaaccgc cgattcaaca
     2101 aaaccctcaa catacccgtc acacaccccc cggcaaaaca ccgcgaagcc gaagccccgc
     2161 agtgtccgac gccgtctgaa aacttattcc gcgtaaaccg gttgcgcgtc tttggggatg
     2221 cccgccgcac cttccaacgc cttgcggtac acccaaccgg ggtcgggcgc ggttatgtcc
     2281 gtttcctgaa aatccaatgt gcgcgccaac aggtagcgtt tgcccgattt aaacgcattt
     2341 tccgacacat agccgttcaa cgtcgccgta acataaccgt tgcggtaatc cgcgccgata
     2401 tgttcgacga cgtggtaggc cgcactcgcg ccggtcatac cgtcctcgac tgcaaaattc
     2461 aaggcaacaa tctttttatc ttcaatcatt tcaaatctcc aaaagcccgc taggggcggg
     2521 aacgaaaatg ccgtccgagg tcttcagagg gcattgcccg gacaaaacag gaaaaaccca
     2581 aacggcggca tacgggggcg tcagccaacc tgccaaacct cgttttcgcg cgattccgga
     2641 atcagcaccc tgatattgcg cagctcgata tactgccacc agtgttccac aaaaaacagc
     2701 aggttggatt tcggaacggc agtattaaac tcctcctgaa acagagaata ctgttggggc
     2761 gcatgacccc ggtatttctt aaccactaca aacttatccg acctgtcgaa cgcaagcatc
     2821 gtactccagt cggcattggt aaaaggccgg tactcgaacg atatgccgta gatatcgtcc
     2881 ggcaactgaa gcagcccgcc gtaagcattc caatgtttat acatctgatt ttggtaatcg
     2941 tgtacacgcc ggtcgccgcc caaataaacc tgcttcttac tgtcgtaccg gaaactttcc
     3001 gcaaaggtgc gccaaaccat cctcccgagc agctgcttgt attcgtagtc ggattgcgcc
     3061 aaatacgaaa ccgacacgaa cggcgaaaac accgattcgt gggaagcgag cgacaacttc
     3121 aggctgacgg gtttgcccgc ggggatgact tgaatctggt actcgaggcg ggttctgtac
     3181 cttgagccgg attgttcggt ataatccccg cccccccttg cccggggggg caggtcttcg
     3241 tacctcccga aagttcggcc gcgccaaata taattggtat agttctcggt ttccctgact
     3301 tccctcggcg caagcacacc cccgttcaac agcagctttg cctgcacctt gttgttgccg
     3361 aacgtttccg aaaccacata aaaattcgga atcaaaaccc tcggcagcgg gtctttatcc
     3421 aaatccaaca cccaaacgtt accctccgtc caacgcagcc tgtgcgccct caacacgtcg
     3481 ccctcgacgg acgaagccct gaccgtgccg ttgaaataac ccgaatcggc ctcaatccgg
     3541 ccgcgtatta ccgcattgtc ggccgtcagg atgccgtctg aagacaccgt aaaattaccc
     3601 ccgccgatat tcaggctgcc gcccgtgatg cttccgagat tggaagatac cgcgctcaaa
     3661 tcggcaacct gaatcttatc ggcagtcacc gcgtttgccc cgattttatc ggcggtaacg
     3721 gcggcagccc cgatttcccg cgccgtaacg cttccggctt tcaggcggtt tgcgtccagc
     3781 gtgtccgccg cgattttgcc gccgtggata tccccggcgt tcaacttatc gacaaccgcc
     3841 ttgccgttga ccaccaattc gccgttgatg cccacgcggt ttttctgcgt gtccaccacg
     3901 aacgggaaca catccgcctt cccggccgca ccgatgccga agcggtcggc attcacgata
     3961 aacttgcttt cgggcgtgcc gtttttcggc gtagtcgcca agccgaagcc cgccactttg
     4021 ccgtttgcat caaccttgac cgtgtattgc gcctccaagc cgttgatgct tcgggcgtgt
     4081 gtctgaaccg ccgccgcatt gccgtccgcc gtcgatcgga ctgcctcgac gcgctcgttg
     4141 acgctgcccg cgccgttgcc gtcaatcagg ttgattttgt cgcgcaaagc cttgttcaga
     4201 ctgctttccg acaggtcggt tgccgatacg tcgtaaacgg taaacgatat gctgccgctg
     4261 attttcaggt tatctttgcc gaaactgtcg taacccgccg ccctcaagta ataggtcttt
     4321 cctttctcca gcggtttgcc gccgcatttg gcgatggtta taaacgtttc cgcgccgtca
     4381 taggctttgt ttgcgtccgc ggccgggcag gctgcgtttt cgcaaaccca aacgataatc
     4441 cctgcaaagt cttcttcgtc aggtttttgg caggtaaaaa acgcctggcg caggccgctg
     4501 tcgatagaga tgccctgcaa tgcctgcaat tgcggatttt gcgccgcgat ttgcgcccaa
     4561 ttgcccgttt taccggtaac ggcacgcccg cgaaccttga agacaatacc gcgtacctgg
     4621 ccgccgtccg ccttcatatc cgcctgagtg taggtgtagc tgttgtcaac gatgccgtca
     4681 accgcacgca gacggcgtcg gctgccgcct gcgtagattt ccacgtcgta ggtatccgcg
     4741 ccgtccaatt tatcccaagc gatgacggct tctttgccgt atgcccacga tgatgtcagg
     4801 cgcaggtttt ggatttgacc gagcggcgcg ccttcgatgg tgtaggaata cgccggcact
     4861 tccgccaaat cctgaatgcc gccgccgaaa acgttgtacg aaaccagttt gacccaaacc
     4921 gtgcggccaa tccagttgcg cggcacggcg tatttgaaca atgcttcgtc gatgcgcgca
     4981 aaccggctgc ccgccgcgtg tgcgttgacg gcggagccgt acgcgccgcg cgtcaggttg
     5041 cccaatgtgt agcgtcccac gcccttcagt tcggcgtcgg cgtatgccag aaattcgccg
     5101 tcgacgtagc acaatgtcag caaatcgcgg ctgtcctgct ccgtgccgcc cgtcagctgc
     5161 cccgccgaaa tttccacgcc caacgtattt gtgcggtcga aaaccgcgcc gtcgggcaaa
     5221 tcggcggtca gcgcgccgaa acgcgccttg cggttgaccg cgccgacgcg ggtgtagctg
     5281 tcgccgtccg ccgataccca cacttcggcg ccgccccaca taccgccccc ggcggttgcc
     5341 agccagattt gcggttcgcc gcccgtcagt tgcaacggcg cttcgaaaat taccggcaca
     5401 tgggcgttgc ccggcgaaac gttgtagtcg gccgaataac ccgaagacgg ctgcgtcgga
     5461 tattccgaca cggtataaac accggccggg cagtcttcgg ccttgacgga taaaacccct
     5521 tctccgtctt cttcgatttc cgtgattcgg acgggcgttt tattcaggcc gagccccgcg
     5581 tcggtcaggg ttacgatgtc catcggctca agcaggcagt atttccaacc cagcttaaac
     5641 tcatattcgt tgcgcacgta cagggcgcgt tgcagcagtt gttgcgccac tttttgcgcc
     5701 accttcgcat cgcaaatgcc gtgcatcctg accgcgtctt tcgggcgcaa tccgtactgc
     5761 tcgatattcg cctggtcttt cgcttcggcg atggcgacgt tgtagtcgtt gtccctgtca
     5821 aggtactcga cctgaatctg attaaacgca tcggcattgg ttttgcgccc ggcctttaca
     5881 gggtcttgcg cgcccgaaac gatgaaatcg tcatcggtca ggtcgtaggc ggccttattg
     5941 tcggcaacgt acaccgcgcc gttccccgaa tggctgccgt ccccgtaggg gatgattttc
     6001 aggcggcctt gcgaaaatac cgccgcgctg ttggtctgct ccagcagttc ggaaatattc
     6061 cgttgcgcct ccccctgttc cgtgtaggca gggcttagga aaatcccgac ggcgcggcaa
     6121 taattgctgt accggtcggt atcgccgatg ttttgggacg ggaaaccgca gccgtagcgt
     6181 tggttcgtca gcaaatccaa tacgatttcg cgcgggtttg catcggggat gttgccggaa
     6241 tagcccgatt tcccgatgac ctcgaaattg tgctgatata tttgcgccga tttcgtcagt
     6301 tcgtagttcg ggctgcacaa ataagccgtg cccgaataat tcaaggcttg gccttggtgc
     6361 ttcgcctgtt gcaggtgcgt ccacaacggc tgctcgtcgc cgccgcgcat aagcgtcagg
     6421 cgcaactgtg ccggcgaatc gaatttctcc ttgtcgcgcc aaacccgccc cacgccctga
     6481 atctcgccct cgcacaaagc aagcatgacg gcggcttcgt aggtatagga aataccgacc
     6541 tgtttcacac cgccgccgcc cttgccgccc cggcgcgtcg tagttttatg ctcgatggtg
     6601 acgaaatcgc cgtaccaaat caaattgccc gccacgcgcg tcctgccgta gatgacgggc
     6661 agggtcagcc cctgtgatga ccgctgaacc tgtaacgata aaatccgctc ttcggcagat
     6721 gtaacggttg acgatttacc acccataaaa ccacctcgaa acttgcaaaa attatcaaaa
     6781 tcgatataat ttacttatat caaaacagat ataaaatatg aaaccgttaa atttttcagg
     6841 cggttcattg gattgcctgc ggcaattccc cgaaaacgcc aaacaggcgg caggttatca
     6901 acttcaagaa aagccggaaa accgcgggcg ccgattcgga atcggcggaa aaacgttaca
     6961 acaaattgat tcgggaaaag aaataatgga aagccaaact ttcgcctccg tatttgacgc
     7021 actgtgcgac acgcctgccg aagccgccaa tatgcggttg cgcgccggcc tgatgatgca
     7081 catcgccgat accgtccgcg aaaacggttg gacgcaaaaa caggccgcag aacattgcgg
     7141 cctgacccgg ccgcgcatca acgacctgct gaacgggaaa atcgacaaat tttcattgga
     7201 tgcgctcgta aacatcaatg ccggactcgg gcagtgcatt tccttatcct tcgcccccgc
     7261 gtaaagcccg tcctttcaga cggccgcaaa atcaaaatga aaatgtaaaa aaccgaaccc
     7321 cgcgcccgat aagttcggct tggccgatgt cgtccgacac cacgccgcgc ccgatgtagc
     7381 tgtgaataac cttgccgccg cccgccaata tgccgccgtg cgaaaacgac cgcccgaaac
     7441 gccatactgc aatgtcgccc gcctgcggcg attccgtttc acggcaaaat tgcgtgacga
     7501 accccaaata acgctcgcaa tcgcggtgca gatgccaatc ttgagggtaa gggcgcgggt
     7561 cgaacccttc gggaagcagt ccgaccgccc cgtagacggc gaccaggagc atcgcgcaat
     7621 caacacccgc gcccttgacc attgcatgat gatggtacgg cgtgccaagc cacgaccgcg
     7681 cctcttcgac gatccgcgcc ctcaaatccg tttcggacgg catatcacac caccgtatct
     7741 gccgaaggga tgaacgggaa gccgcgaaaa tgcacgatat tgtcaaactt atccttgcag
     7801 gtatcgcgcc gcttgtcgca gcccggataa accttgaacg catcgcccgc ctgcggcggg
     7861 tagggcaggc gaagggcaag ttcgaacgtg ttgccgccgt gtgccttgac cgtcctgctc
     7921 aaacccgcgt tgcgcccgcc cgcaaactta atcacgccct gcgaaaacca gccgtcaggc
     7981 tgcgtcagat tgtgcttcag cacggttccc gtccggctgt tttcggttac gcgcccgttt
     8041 accgtgaatt tctcacggtt gaccttgcag ccgtcgtcat agagcgtcct catgcagccc
     8101 gcctgataaa tgttgcgcgg gctggaaacg ttcaaaagct cgatgtcgga tttcacgtca
     8161 accttcacgg acgacctgcc gcccgatacg tccgacacgc gccctgaaaa aatattcacc
     8221 gcaccgacgg ggcgcagtcc ggcatcaaaa aacacacggt ctattttgac ccgcgcgccg
     8281 tccaatacgc cgcccagggc ggcctccgcc cattgcaggc cctcaagcct gtaatcgggc
     8341 gcggctgaaa tctgcagggt gttggaatcc acttccaatc cgacggcggt acgggttgcg
     8401 ccgcgcttga taaccagctc gtgcgccccg taggcctgac cgtcccaaac gacgggcata
     8461 tccgcgccgg tatgccgcag cacccggccg cccgaaagcg taatggtgta caaatccgcc
     8521 atctgaaact cgtcgccgcc gtgcagcaaa tcaatcagtt cttttgtcgc cgccttcata
     8581 gtttcacact cgtaaactca atctttttgg ccgcccacaa gctgcccaaa acgttttcaa
     8641 aatccaccgt gtcagacgta aacctcacgc ggaaataaaa gccgcccgtc catgtgatcg
     8701 ggcggcccgg cggttgcggc gtgttgaaaa ccaaaacgcc cttgtcggta acggtgtaat
     8761 cgcgcccgta cgccaacgcc gtgccgccga ccttgacggc gggccgttcc ttgaccgccg
     8821 acacaggctc gataaatccg cccatcgaac ggacaagctg ataacgcgca acgccctgca
     8881 ccgtgtttcc gacaggctgg tcggttacgg cgttgtcggc cgggtcttcg taaaggaaac
     8941 tttcaaagct gcctttgcgg gcgttgaaga atcccgccag tttttccaac tcgtttacgg
     9001 acgcctttgt ccgcaatacc tcgaacgaca gcgaaaaccg ccattgcggg taggtgtagt
     9061 aggcggttcg gaactcacgg ccgcttgccg atttctgcgt ccccgtactc cataccgccg
     9121 ttttcttccg cccccacttc aagccgggga actcggggaa aaccgcattg cccatcagat
     9181 gattcctttc gctttgagca gggcgtcaaa ttcgtcttcg gacagttcgc cgccgccaag
     9241 cataccgacg gcttcggctt cgtccgcttc gctttgcaca ctgcccgacg acggcttgac
     9301 gcccatatac gacgctgcca agatatgcac gggcggatgt tcgcgccaat actcgttcag
     9361 gtgtttgata cgcggcaaat ccaagttgtc ggcgacgtag tcccacgtcc agccggtaga
     9421 ggcgcaaacg tgggcaatca ccgcgccgaa acttaatccg ccgccctggc ctcccccgct
     9481 tgtgcggctt cctgctccct gcgtttcaag cccgaaacgt ccatcacggc ggcaaacact
     9541 tcgttcatgt tgccgatatc gatcaaatcg gccgcttctt cgcgcgttaa atccgggtaa
     9601 ttgcgcttca gggcggcgtg ggcgcaatcg ataacggtag agatttgtcc ggcatccgcc
     9661 gcattgccgt caaatgagcc gatgcgctcc tgcaactgct ccaacgcgcc cagcgcaata
     9721 ggcggaatca cataatccgc gccgttcagt tctacggtta cgcctttaat ccgtacggtc
     9781 atttttattt ccttgacttt ggttgaataa aaaaagccgc cttttcagac ggcatataaa
     9841 cattactcct gaatccacag cgtaccgatt ttaaagcccg cctcgtcggt tgaggcggta
     9901 aagtcgattt cggggacgga gaagtcgtcg tttttagtcg agaacaagcc caatttgccg
     9961 ctggttacgc tttccagttc caacagggct tttttgcctt tgaactgcgt caggtacctc
    10021 atcttaaacg tcggcgtgtt gcccgtcgcc atattggtca gctcgatttt cttcgccggg
    10081 ggcatcgttt gcgtataggt aaagctcgga taaacggttt tgcctttatc cgagctgtta
    10141 aaggcgtaca cgcccgattc cgaaaccgta tattggcctt gtttcgggga ggatgctact
    10201 ttgatataag ccgtgccgtc ctggcccatc acgcccgcgt cctcagcgaa cttgccgccg
    10261 ccgggcgcgg ctgcctgaag gtaagaataa ttgtcgccat cgaggacttt gcccgtagtt
    10321 tccgcccaaa gcgccttcat cgtgccggtt gcatattccg taccgaaaaa cagggtattg
    10381 agggtcaggc cgttaatcaa cgcgcctttc attttgcccg acaccttgac cttgccttgc
    10441 gccacagcca aaggataacg gttttgaccg tagaactctt tcaattccgc cgacaaatcg
    10501 acggacattt cctgcaagcc cataatccgc acgggcgttg cgttctgtac acggttgccg
    10561 taagcatccg taatcatttg ggcgaacact tcgccgctgc caaacgtcaa ctgcatgaca
    10621 tccctttcaa aaaaagccgc attacgcggc gcaaatcaca atcggaataa tacaaaccgc
    10681 ctgttcgcca agcgtcccct cgtcggtttc caccgtaccc tcgacgcggc aatactcgat
    10741 atccgcgccg tccgccgtta aatccgtctt gcccgtaacg gggtggacgg cgttcacggc
    10801 attgcacacc gcgtcaatca gcggattcat gacgggcgcg ggcggttggc ccgccgtctg
    10861 gacgtacagg taaacatcgg cgcgcaaaat ccacttggtt tcctgccccg tcaatgtcaa
    10921 cgcctgcata tcgccctgtg ccataaataa cgcgggctgg tcgtagcgtt tcacatcgtt
    10981 ccagtgcagt aatttacggc tcttggtaac aaaaccgcct aatgcgtcca gtttcgacca
    11041 aagcgcggaa taaaccgcct cacgattcat ttcagcacct ttccaatcga tttctgcagg
    11101 tcatccgcga acttcggcgt caaatcgcgc aatgccgaac ggaggaacga ccgctccggc
    11161 aacttcacat cacgggtatg ggcgcggacg tgaacatatc gcggcggttt cagcggcctt
    11221 ccgaacgcct gcctgacctg gcgcagcgaa gccttcacat tgacattgcc cgcgaaacca
    11281 tactcatgcg cgatgccgta acggacgttc gtgttgactt cgcccgaaac gacattgccg
    11341 gaaacgttca cgcgctggtg tacggagcgt ctcaggttgt ccgtccttac cctcaacacc
    11401 tgacccgata accggttcag cataacttcg ctttgcaggc gcaatgccga cctgcccact
    11461 gatttgacga ccgcatcttg aacatcggcg gcgtatgcct taaagaccgc cgccagaata
    11521 ccgccgccga taaattccat cttcagcatc acacgccctt tcgtttgtac tcattgagta
    11581 tcgcaaacgc cgacggcggc ataccgccgg aattaccgaa cgtagaaaaa gcgacggttt
    11641 cgcctgcaag cgtcttgctc cgtacgccct tgccctcgat ttcgttcacg cgctgcaccg
    11701 cgataatcat caccgcttcg cgtatgtcgg cgggcatgcg ctcataaccg gcgcggtacg
    11761 atacctcgac atttttcagc ccgcgcgcga aaccttcggg gcgcatcagc agccagttgt
    11821 caaactccca gccgccggca tccgcgccgt tgattttgac ggacgacacc gacaggacgg
    11881 gataattgtc caacacgatg cggtctttgc cgttgccgtc gtaccgcccg acataatcgg
    11941 cggccaggag gctgcgcccg atataggctt ccgccgccgc cgacacgccg tcaatgacgc
    12001 tgaggaagaa atcgtcccgc cggtcatgtt caacgccgat acgctgcttg aactcctcaa
    12061 gcgataccgg ggcggccatc gttattcagc cttttcggct tcggcaggtt cggcttctgc
    12121 ttcggcaggt tcggcagctt cggcttcggc ttcggcaggt tcggcagctt cggcaggttc
    12181 ggattgtcct gccggctgcc cggttttagg tttacgcccg cgcttggctt tttcaggccc
    12241 ttcggcaggc tcggaggcaa cgttgccaaa accgaactga tacaggaatt ccgccgcctc
    12301 cgacggcact tccacgatac cgttttcatc tgccgcgtag ctttggctgc cgaaggaaac
    12361 gtcggtaaac ccttcgggcg cttttaattt gactgtcatc tcaaaatctc caaaagaaaa
    12421 agccgtctga ttttcagacg gcatatcggc ttaacccacg ttggtaatca taccgaaggc
    12481 aggcatgaac ataccttgca gcacctcgtc cgcatagacg ccgtattcgt acatacgggt
    12541 gcgcagcggc cattcgattt ggtaatactc ttggcgcgta cgcacttgca gcagattgcc
    12601 gacgccctga acgtaggcgg gcaggcggct tgagtagaac aggtaagtac cggcaggcag
    12661 gttcgggtgt accacgatgt tcaggtcgtc gcccgtgatc ttgttcatat acgaaccgac
    12721 aacgacaccg gcgcggatgt tcgccgtatt gttcacgtca acgttcaact taatcaacgg
    12781 cgcgccgctg tttccgataa tcagcttggt cagcgccgcc aaatcgcggg cgttgacgta
    12841 gacggtatcg ggggacaggc ggtatcgggt aaagaaatgc gcgaacgcct cttcaaattc
    12901 atacacgccg cccgcgttgt cggaagtcag gccgccgcct ttgttgtccg accagaacgc
    12961 gcccgaatca ggcagggcga tttgggtcaa caggccgtca aattccaaaa tagaagtgga
    13021 attgtcttcg gacggcaggg aagcggcggt ctgggtacct tcggcatcag ccgaaatttc
    13081 cactttggcg gcagtggtaa ccgcgcccag tttttcagaa ccggccgcac cccagtacca
    13141 agcataggca accgcgccgc gaacagccgg aatcacggcg gttacttttt tgcctgcctc
    13201 aataccggaa acggaagccg ccgcagattt tcgggcggaa ccgccgccga acgtatcggt
    13261 tgtaccatcc gcgtttcggc gtgtgatttt ggcagggact tgggcagttt taatgttcgg
    13321 gctttgtccg gttgcaccgt tgttcgcgcc tgccacatcc cagtatgcct gcaagcccaa
    13381 agccacacag attacagaca aggtgctgac gctgattttc cccgccgcgt cggcggaaac
    13441 gacggcggtc ggtgtaggtg taacgcctgc cttcaggctg gtgttgccgc ccagcaaaat
    13501 catttcttcg gcaaccatag tagcctgaag ggtttgggcg accgccaacg ctttcacgtc
    13561 ctcgaaaccg cgcgcggcgt aatccgcttc aaaggttact tggttttcca agccgatggc
    13621 gcggaattgc gcgttgcgtt ccaccgtttc gtgattgatg acgccgccgc gcctgccttc
    13681 gccgataccg gcgcgttggt tgccgacgtt gatattcgtg acggctttcc agttcgagcc
    13741 gatggtgcgg ccgccgccca cgcgcgggat acggttgcgc aacggggtca ataccgggta
    13801 gagtttttga gacggcgcgg aaaggtcata ggtttgcagg ccggcggtaa agccggccgg
    13861 ctgggtaaaa cctttgtcag gcggcccgcc gcccgcttgt gccgacttca tcagttcaat
    13921 cgtttcttgt gtgagttggt tcacgttcat ttatcgctcc tgataataaa aaaaaccgcc
    13981 tgtaagcggt gttacagacg gcctgtttgt tttgccttaa tgagtgtcgc cacgtcatcc
    14041 agcgtgccgt cattctttac aatcggctga aaacctttta acgggtcttc gccgttatct
    14101 tctgccttac tgatagcttt cgtactgcct ttcggcggta ctgcctgttt cttcaggctt
    14161 tcgatttccg cctgtgcttt ggcaagggcg tcattcgatt ttttcagcgc gtcttgtgcc
    14221 tttgccagtt cgtcagccga ttccgctttg gcaagatcgc ctgatttgcc ggctttggcc
    14281 gccaaaccgt cggccggctt atcggcttcg cttgccgcca acgctttcag cgattcggca
    14341 aggccggccg ccgattcttt gatttgcgcg gtaacggctt catcgatgtt gtcgtaggat
    14401 gcgtcctcaa ccagccattt cagcgacatc aatacatcag ccggtgattt gacttggtac
    14461 attgatttgg cggccggccc gcctttcggc ttgtcggctt tagccaatac cgctttcaag
    14521 gcggcgattt cagattcgga caattcgacg cttgccgatt ttccggcttc gtcctttttg
    14581 ccggcgtcct tatcgccttt atcttcggtt ttaggccctt tatcgccgtc tttaggattt
    14641 tcatcttcct cttcggttgg tttgtcagac ggcttgccgt ctttatccgc cgcccctgcc
    14701 tcatctttcg gtttgtccgc cttaaagcag gtaaacaccg cgtcgggatt ggcagggcgg
    14761 tcgacaaggc tgatttccgt cagcttcaag cccgtgattt gcgacttgtt caaatcatcg
    14821 cgggcggtaa ccctgccgcc gatggaaaag cctttgtaaa cgcctgtctt gactttcgtc
    14881 acggcaacag ggtcaacgat atgcgccccg aaaaatgtgc gcccgtcgtc ttccacgttg
    14941 atttcaatcg ccgttcccgc cgcgtttgag ccgtgcatct cgcgcaccgc gccgaacttc
    15001 atataatcgg gaatcgccgc cttcattgct tccgccgcga cgacttcgcc gtccgaatcg
    15061 accgcttcgc tcgaggcata cccccaaact ttgaccgtgc cgtcgtcctg cgcctccgtc
    15121 ttggcgattt gcgcgtataa cttcgtcatt ggtttgctcc aaaaaaaagc cgccccgcta
    15181 aggggcggca aaacacaccc gcctgacccg aaggaatcaa gattcaggca tatcccctgc
    15241 caaaacaggc acaaccgcgc accggcagtt cggatgaccg ggtatcgtca acgcaccgtg
    15301 ggagaaaggc tcgtgcaaac cgattacgcc catcccgccg ttggcattgc agacatcgga
    15361 caccttgtcg tcttttgcgg ttatccactg cttgccggca accagccccg tttcttccca
    15421 gcctatcagg ttgcccatac cgtccgccat cgccgtttcc gttcgggcaa tggttcgggc
    15481 gcgtgcgttg ccgaaagcat gggattcttt caggcggcct gccaattcct gcacactgtc
    15541 gccgtttcgc atggcttcgg ctacttgggc gcgtatcatt tcgcgcgtcc cttcggtgat
    15601 ttgccactcg gcggcaggat ttcggacaag cccgccgccc gcccgcttca tgccgaccat
    15661 ttcggcggcg cgttcatgcg cccacttgac ggcgcggctg cgaatgttcg taaccatacc
    15721 ggcggcaggt tcaggcatta cgcgcaacaa ggcggcaacc gccccgtctt ccgcaacacg
    15781 cctgattatc ggctcgacca catcggacaa tcccgaccaa tcgccaaaat ccaagccgtt
    15841 tgcaacaacc cctgccgcgc ggctcaattc cgccgccaaa tccccggcct gccaatcgac
    15901 ggccgcaccc tcaatcagcg ccgcgatttg ttcggccaag ccgtcaatgc gtgtcagcaa
    15961 ataagcctca ataagcgcgg cagattcgtc ttcgctcatc gggctttccg actttcccaa
    16021 cttttcagcc ccttggttcg gcggctcttc gggctttcgg ccgtcttgct tatccggttc
    16081 aggctgcccc tgccccggta acggctcctt gcccagttcg gcgcggattt cgtcggcggt
    16141 caagatgcct gcgtttttgt agatggcgta gatttcagcc tgttctttcg ggttgagcga
    16201 ttcctccccc ttccagacaa actcatacgc cgccatatcc atgtaacggg caagcacgtc
    16261 gtcaatcagg gcttttaccc agtttttcag gctgcccatg ccgtctgaaa gcgactgctc
    16321 gcggctcgtc tctgccacgc tgcggtttac ctgcgccacg aacggcgtag gctcgacact
    16381 aaacgcaaag cagacgacac gcgccagcca ttcgtcgtaa acgtccttca acggcggctg
    16441 cttcgtctcg cggaagtttc gggacaactc gcccggcacg aaacgcattt tgcgccgctg
    16501 cgccgtttcg cccgacagca gcaaatccca gtattcttga aaccgcctga tgtcgtccgc
    16561 cgaccacgtt tcaggcacgc cgactaaagc atcgggcacg ctgcccgccg tgtagtattc
    16621 cagcgcgtga acctgccgtt ttagggcgat attcacggtc atgatgattt gctcgacggg
    16681 cgaatagccg taaaccttgt aacttcggtt attccgcgaa cggtaaatca actcgtcagc
    16741 cgtgtaatcg accgccgcca tgccgtgcag gatttgctga tacgccgtat cgggcggtaa
    16801 cggcatacgc cccgtattgt ccaaaacgcg cttaatcgtc gccccgtcca tcacttcgag
    16861 ggcgtacaag ccgccgccca gtgttttgcg cgggtagatg cacggcgcgt caataacaaa
    16921 caggtcttcc agcaagatgc gcagccagtc cgcccacgta tgttctttat cgggcgaccg
    16981 gaagaacgca acggcttcat cgacctttcg gtctttccgt tgcgattcgt cgtcttcggt
    17041 tgattcgacg tcgcgctttt ggattgtcca cttaaggcac tccatttggt ctttacgcgc
    17101 ctcgataacc aaacgcaata catcgtagtt gtcggcaagg gcgcgtaatt gcgcaaagcc
    17161 taccgcttca cgttcgcgcg gcttggaatg cccgacgttg tagaacggct cgtaatcaaa
    17221 ccgccgcccc tctgcctgct gcgcggcagg ggccggaggc tcgcccgcgt cgaaccaccc
    17281 gtccgcgttg ccggtaaagg cgtaacggac gccggcggca acgcgggcaa taaagccttg
    17341 cgataaaggt gtctttttac tcatttcaca gcctcgacct gcgatcgcag gaaatcaatc
    17401 atgcccgtcc gggtatccag cagctcgcca aacgcacggc ccaaacagtc tatttggtcg
    17461 tcatgccggc cgttcgggaa catgcgcatt tccgcaatca gcgcgtccgt gtcccatgtg
    17521 ccgtcgtcca gcaccatcac attgccgatg ttgacctgtg ccgcgaacgg tccggcgcgt
    17581 gtaaccttgt cgcccgattc ggggccggcg gatacggaaa aacccgccaa ctggcgggtc
    17641 aggtatagtg tttgggattt gcctgcctga ccggggtctt gcgggatgga tattttcgtt
    17701 ttcacgccgt ctttttgcgc cgtgttgcgt aatatcctgt cccgctcgtc cgcgccgtac
    17761 cggccgcgca cgacgttggc gatgatatac cgcccgtctt ccgtaacgcc aagcctgccg
    17821 cctgccgtgt agtcgccgcc gtttgcggtt gaagccaagt cccacgcgcg tacccatctg
    17881 atattcccgg caggcaacgc cttgacaaat tgcaggttgt caggcttgaa cgtaccgccg
    17941 tcaggcgggg cggggcgttg caaatactgc ccggcaaaca catacggcgc ggcctgctcc
    18001 attcggcgca gtgtttcaat atcatgcttt tcaggccaca acgccgtgcc gtcttcctga
    18061 atggcaggca ggcacaaatg ctcccactct tcgccgttgc cgccgtcaag cagccagccc
    18121 gccaagtctt tctcgtgcag gcgttgcata atcaggataa tcggcgtgtc agggctgttc
    18181 ttccgggatt cgaccgtgtt ttgaaaccag tcgatgacgt tctgccgcct gacctcgctt
    18241 cgcgcttcat ctgctttgtg cgggtcgtca atgatgatgc agccgccgaa tccctcccga
    18301 tgcctgcccg caccgaaacc tgtaatcgta ccgcccgcac ctgttgcgta catcacgccg
    18361 cctgcggttg ttttccagtg atggccgctt tcgcctgcca gtgccaaatc aggaaaaatc
    18421 gcccgatact cttcgtgttg caccaagttc cgaatctgta cggagttatt gaccgccagc
    18481 gccgccgaat agctcgcgtg gataaactcg caatcaggca cgcgccccat cgcccacgcg
    18541 ataaagttca cgaccgcgat ttccgttttc gagtatcgcg gcggaatatt gataatcagg
    18601 cgtttcgttt cgccgttgaa aacacgctcg agcgcgttgc agattggcgc atggtgattc
    18661 gcccgctgcc aaacatagcc gcgcctttga taaaacatcc ggcgcgtgaa catatacagg
    18721 cttgcagaac ttaaactgcg aattactgat gtttcaacat cgtcgaattg ccctagtgcc
    18781 atttcattct aacttccttt ggagaaacgg ccatacagcc cctcttatga ttagcgtttt
    18841 atgctaaacc ctacgcaaca catcttccgc tatcttgcga tattcctcag catcaagccg
    18901 tacagccgcc ctcatgctcc catcgcccga tttaacgtca agctccgtct tatcgccgta
    18961 tttcttcggc gcaatcttgg aagccgccca ctttcgggca tctatctgca atttcgcctt
    19021 tgaaaccgcc gcgctctctg cttgtgcact atcggcaatc tcgatgattt cttcagcgaa
    19081 atagtccgcc tgcttttctc tcgcgcgcgc gtattggtgg cgaaattctt cattagcttc
    19141 caaccaacgg taaatagttt tcattggcgg cacaccatct tcggcacata ttgaacgtag
    19201 gcttctgcca tttgcgattt tttcgcatat cttatcggcc atgtctttcg tgtaatctgt
    19261 cggacggcct aatttgcgtt tcgtgccgct cataaaccct cctcaaaaaa gaaaccgtct
    19321 aactctgacc cctctcagaa ttagacggca aacacacact cgccacatag gaaaaacgga
    19381 acgccgctac ctgtacaggc agaagctcaa attcggacag cctaaaacgc aaaaacccgc
    19441 acattgttat gtacgggctt aaaaattcat atccttcggg cgtgcgaaaa tccccgcaag
    19501 ggtaacgatt tgaattatac acctattgcc ggaaaaaaca acaggccgtc tgaagattca
    19561 gacggcattt gtattcccta ctgcatcaaa ccgccgacag gttgcggatt tcgggcagta
    19621 tcgggcggat tttttctgcg tgttccgcgt cggcgtgtgc gcttaaggct tcgagggcgt
    19681 ttgcggcggc tttgaggcgg ctgcgcgttt ccgcccagac cgtccacatc gtaaccgcct
    19741 gcctgcagcc gagctgcttc agcggcaggg aaacgtctct gcccatttgg atggcccgcg
    19801 cgccgtagct gacggcgacg gcgaggtcgt acagggcgtt gccgcttatg ggcagggcgg
    19861 gttgcggcgc gggtaacggt tcgcggtcga ggacttcgcc cgtcaagccc gtgtgcagtg
    19921 tcaatgcgtg gacgtaggcg acggcttcgg gcagcttccc ggcggggatg ccttcgacgg
    19981 attcgacgtt gaagcgttgg tgtatcatac tgtacgcgga ggagtagtct atgcctttgc
    20041 gtccgacaag cgcggcaaca gcttggcgca atccggtacg gtcgtcggcg gtggtttttt
    20101 gtccgacttg gtagccgcct gttttgcgga tggtgggcag gacttcggat gttacccatt
    20161 tgcggaattt ccaagcggtt gagccttgtt ccattgcttt acggctgcga agaatcaaaa
    20221 tatataaacc gctttcgttg atgatgttca cgttaccgcc gcctccgtga atttcagacc
    20281 gccctatgtt aaacatagac cgctcgtcat catctaattt ttcaagtgct tgcgttgggt
    20341 tctggatttc taaagcctta catacatcgg cggcaacaaa ccaagtcaag ccgtcttttt
    20401 caaaggcacg aacgggagaa gtagtattga aattaaatga ttgaattacg ttcataatga
    20461 agtttcctag tgagttttct taatgcccgt tagggcggac gcgtggttaa gaaccctcac
    20521 tagatgggcg gacttattcc ccttacgggt attgtattcg tcgcccacgc gtccataaga
    20581 aacttcggtt gtgccatcga aacaaacaac acgaaaggaa acttacagat atgaaaaaat
    20641 cacattgacg gagtgattgc cgctagtgtg tggttcttac gccacgaaca ggaatataaa
    20701 acaaaccccc tgcacatgca aggggttttg ttaccttatt tgggtttgcg tttaaaattc
    20761 tcgtcatcac aacagaatcg gtaagaatca ataaccaacc aagcaacgcg aagcagattt
    20821 tcaggcgtat caataataac ttgattgcct gatacctcaa gtccgcatcg ttcgatattc
    20881 gaaatatctg actcttccaa ttcgataggg aagattacag acggcctttg tttgttgtca
    20941 aaataccgta atatccacct gttactttta ccatcaacca atacgccgaa atagctttcc
    21001 gtatcttttg cctcaatact cgcatcatca ggaagaattg atttaaccaa atcaaacaac
    21061 cttctttctg aataagtggt aacgatttta ttgttttcgg ggtcgataat cggcgcggtc
    21121 gggtcttcct gctccttctc aacaggggcg gcctcctcct gcactttcgg cgcactcagt
    21181 cccgatacaa ccattgaact aaccgtattt tgaacagcct gtttgacgat atgacggatg
    21241 ctttccaaat atcgttgggt aaactgccgt tgaatattcg cccttcccgc cacataacgg
    21301 acgaaatcca aatccacctc tttcaagctt tctgtaattg attctgtaaa tgccgtcagg
    21361 tatatgcttt cttccgcaag gcttcgcaat gcatcaggtt gaaacttgtc gtgcctgaac
    21421 tgatacagtt gcgccatatc attttcattc aacatcgtaa catcaacggt caaaaacggc
    21481 tccgaatcca taatattctt gttggacaaa tcggtaaaaa atcgccattc ccggccgttg
    21541 gtaatcgcgc aaatggctat ttcaggcgtg gcattgaagt aacgcgacaa ctgcggacaa
    21601 tgattggaca aattttcagt atatgatttt gcttcgataa acataaccgg tgcgccgttg
    21661 caaaacaatg cgtaatcgac ccgctcccct gattttgcac ctgggaaatc cgcctggtat
    21721 tccgcccgaa ccttattcgg atcgaatgcc gaaaaaccta gaatatccaa taaaggcaaa
    21781 atcaaagcct gttttgtcgt ctcctcggta gtacagatat gtgcaacctt cttgacatgt
    21841 tccgcatggg aagcaatccg ttctttaaat accgcactta cagccgccgc attcatgtta
    21901 atctccttgg ttaaatttga ttgggcaaat tcccacccca ttaagaagtg ttaattttcg
    21961 caatcatagc gcaaccgaaa agaaaatcaa accttctcaa acagcaaatc aaaatcattc
    22021 cctgccgcct gccgtatcgc cctataccac gcggccaagc ccaaatccgt ctgcaaatgc
    22081 aggggcccct cgccgcgccg cctgatttca gcctgcaaac gttcctcgta ggctgcctgc
    22141 gacttcgcac cgatgccgaa cgaaatacgg acggcctctt gttgcggcgc atccaccttc
    22201 gcccatgcct gcaaagtaag aaacatggca tcttcgccgt atctcaagcc gatttcaggc
    22261 ttatgcgggc aagcttcctc ccccatataa cggccttcga tactcaaaca tctatttagg
    22321 ctgcgcgtat cacggtaacg acgctcaaaa gcacgcgcca agtcgttcat aaattcaaat
    22381 tcttgttgat tcatagcttg attaaccctt tttcatgcaa caaaaccaaa gtccgcatta
    22441 caccttccgc gaaggccgtc tgaagttccc cttccgcaca atccgccaag cggttctata
    22501 catcaaaact cccaaatgat tccaaattct accgcagccc gcgcctgcac cgtttcggat
    22561 atggcggacc tcggcaggaa cgccgaaccg cccgcaattg cggcaaacaa tacaacctat
    22621 atccgccact ttttgcagat gtgatttttc cgcttcggtt ttacttttca tccaactccc
    22681 ccacctccac cgccaaaccg ccgcccgtta ccggctcgtc ggcgtaatcg gcggcaatgc
    22741 gccgcacctg cctgtcgttg tgataggcaa cgccttgaag cgcgtccagc gcaac
//
LOCUS       DAXFZR010000030        21933 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000030 DAXFZR010000000
VERSION     DAXFZR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21933
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.030"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..454)
                     /locus_tag="ACFJAS_000974"
     CDS             complement(<1..454)
                     /locus_tag="ACFJAS_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC3982251.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHK"
     gene            706..3168
                     /gene="lon"
                     /locus_tag="ACFJAS_000975"
     CDS             706..3168
                     /gene="lon"
                     /locus_tag="ACFJAS_000975"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC3982252.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            3350..3619
                     /locus_tag="ACFJAS_000976"
     CDS             3350..3619
                     /locus_tag="ACFJAS_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC3982253.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(3796..4230)
                     /locus_tag="ACFJAS_000977"
     CDS             complement(3796..4230)
                     /locus_tag="ACFJAS_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982254.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(4223..5530)
                     /locus_tag="ACFJAS_000978"
     CDS             complement(4223..5530)
                     /locus_tag="ACFJAS_000978"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC3982255.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            5726..6097
                     /locus_tag="ACFJAS_000979"
     CDS             5726..6097
                     /locus_tag="ACFJAS_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC3982256.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            6174..8096
                     /locus_tag="ACFJAS_000980"
     CDS             6174..8096
                     /locus_tag="ACFJAS_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3982257.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFDFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            8083..8478
                     /locus_tag="ACFJAS_000981"
     CDS             8083..8478
                     /locus_tag="ACFJAS_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC3982258.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGLL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            8569..9081
                     /locus_tag="ACFJAS_000982"
     CDS             8569..9081
                     /locus_tag="ACFJAS_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982259.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(9457..9639)
                     /locus_tag="ACFJAS_000983"
     CDS             complement(9457..9639)
                     /locus_tag="ACFJAS_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982260.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            9639..9974
                     /locus_tag="ACFJAS_000984"
     CDS             9639..9974
                     /locus_tag="ACFJAS_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982261.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKAVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            complement(10011..10670)
                     /gene="ung"
                     /locus_tag="ACFJAS_000985"
     CDS             complement(10011..10670)
                     /gene="ung"
                     /locus_tag="ACFJAS_000985"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC3982262.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            10751..11158
                     /locus_tag="ACFJAS_000986"
     CDS             10751..11158
                     /locus_tag="ACFJAS_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC3982263.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDIGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            11173..12120
                     /locus_tag="ACFJAS_000987"
     CDS             11173..12120
                     /locus_tag="ACFJAS_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC3982264.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(12227..13297)
                     /locus_tag="ACFJAS_000988"
     CDS             complement(12227..13297)
                     /locus_tag="ACFJAS_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC3982265.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            13482..13757
                     /locus_tag="ACFJAS_000989"
     CDS             13482..13757
                     /locus_tag="ACFJAS_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC3982266.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            13759..14382
                     /gene="lipB"
                     /locus_tag="ACFJAS_000990"
     CDS             13759..14382
                     /gene="lipB"
                     /locus_tag="ACFJAS_000990"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC3982267.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            14375..15358
                     /gene="lipA"
                     /locus_tag="ACFJAS_000991"
     CDS             14375..15358
                     /gene="lipA"
                     /locus_tag="ACFJAS_000991"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC3982268.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            15833..16297
                     /gene="bfr"
                     /locus_tag="ACFJAS_000992"
     CDS             15833..16297
                     /gene="bfr"
                     /locus_tag="ACFJAS_000992"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC3982269.1"
                     /translation="MQGNQAVVDYMNELLSGELAARNQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            16325..16798
                     /gene="bfr"
                     /locus_tag="ACFJAS_000993"
     CDS             16325..16798
                     /gene="bfr"
                     /locus_tag="ACFJAS_000993"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC3982270.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            17124..17444
                     /locus_tag="ACFJAS_000994"
     CDS             17124..17444
                     /locus_tag="ACFJAS_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3982271.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            17493..20051
                     /gene="glnD"
                     /locus_tag="ACFJAS_000995"
     CDS             17493..20051
                     /gene="glnD"
                     /locus_tag="ACFJAS_000995"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC3982272.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPAPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPVRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            20198..21661
                     /gene="guaB"
                     /locus_tag="ACFJAS_000996"
     CDS             20198..21661
                     /gene="guaB"
                     /locus_tag="ACFJAS_000996"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC3982273.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAVGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(21733..>21933)
                     /locus_tag="ACFJAS_000997"
     CDS             complement(21733..>21933)
                     /locus_tag="ACFJAS_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982274.1"
                     /translation="IWRPAHHFRPIETPVLKDCVYFHRRFKGRRYSADGVQTAFRRHF
                     HSFGVSVRMALPVRAISPCGLP"
BASE COUNT         5828 a         6067 c         5345 g         4693 t
ORIGIN      
        1 tcttatgcca ctcatgcaca cccataaacc ccagcagcag attgagcggc agaaaaaacg
       61 gtttgccgtc cgcctctccg tacacgtcga gcaaagcggc gcgtatatcg ggcgcgcgtt
      121 tgttgtccaa cacaaaaccg gggcggattt caacggcaag catattcaga atacggctct
      181 gttgcgcctg cttcatacgg tgggcgtgga aaagggcggc gatatccgca tcggaacgga
      241 cggcggcagg tttgcggacc ttcttcttca ttgccgaaag cacctgtttg gcattgtgga
      301 aatcgccctg ccagacagtt gcaatatttt gataggtagc cttcaaaatg ccgtctgcac
      361 cgctttcacg gacataatac caacctttgg gcggcttttg cagactttcg ttgcgccatt
      421 cgaacccgtt atcgggaaaa ataaaagaag acatgggata cctgcgtcat gttttgaaaa
      481 tagggcggca gaaccgcaaa ccatacggat ggtacagcaa ggagcggcaa cacagaactg
      541 tttttttgtt cccgccttgt ctttccaagc ccatgccgtc tgaagccgga atgtttcaga
      601 cggcatggca tcaaactcca taaataaacc gcgtatgctt gaaataatac tttcaacccc
      661 aatttacgcg gcaatccgta tcctgtcaga cacaagagag tatccatgac acaaaaagaa
      721 aagcattttg aggaatatgc tgctttggcg acccttcctt tacgggatgt cgtcgtttac
      781 ccgcatatgg ttctgccgct gttcgtcggc aggccgaaat ccatcgccgc actggaaaac
      841 gccattaccc gcgaggaacc ggttttcctg ttggcgcaaa ccgatgcggc ggtagaagaa
      901 ccggttgccg ccgacctgta tcagaccggt accgtcgcac aagtcctgca agtgttgaaa
      961 ctgcccgacg gcacggtaaa agtgctggtc gaagggctgt atcgcggacg tgttttgacc
     1021 atcgaagaca cgggcgggct gttcgtttct catatagagg cggtcgtgga agaagacacg
     1081 ggcggcaata ccgacctcga agccgtgcgc cgcaccctgt tggcgcagtt tgaacaatac
     1141 gccaaactca ataaaaaaat ccctgccgaa attatcggca gcatcaacgg cattgccgaa
     1201 aacagccggc taaccgatac ggtcgcagcg catttgcagt tgaaactggc gcaacgccaa
     1261 cagattttgg agattcccga aatcggcaaa cggatggaat tcctgctggc aaagctggaa
     1321 tccgaactcg acattatgca ggccgaaaaa cgcatacgcg gacgcgtcaa acgccaaatg
     1381 gaaaaatccc aacgcgaata ttatctgaac gaacagatta aagcgataca caaagaattg
     1441 ggcgaagaag acgaaaacgg cgaactggat gccttggagg caggcatcaa aaaggcgggt
     1501 atgaccaaag aagcggaaga aaaatgcctg tccgaactga aaaaactcaa aatgatgccg
     1561 ccgatgtctg ccgaatccac cgtcgtacgc aactacatcg acaccctgct cggactgccg
     1621 tggaagaaaa aatcccgcgt cagcaaagac atcgccaaag ccggactggt gctggatgcc
     1681 gaccactacg gcctggaaaa agtcaaagaa cggattttgg aatacctcgc cgtccaaaaa
     1741 cgtatggaca agctcaaagg tccgattctg tgtttggtcg gccctccggg cgtgggcaaa
     1801 acctctttgg gcgaatccat cgccaaagca acggggcgga aatatgtccg tatggccttg
     1861 ggcggcgtgc gcgacgaaag cgaaatccgc gggcaccgcc gtacctatat cggctctatg
     1921 cccggcaaga ttttgcagaa tatggcaaaa gcaggcgtaa gaaacccgtt gttcctgctc
     1981 gacgaaatcg acaaattggg cagcgacttt cggggcgatc ccgccagcgc gttgcttgaa
     2041 gtgctcgatc acgaacaaaa caacaagttc gccgatcatt atgccgaagt ggattacgat
     2101 ttgagcgacg tgatgtttat tgcgacttca aacagcctga atatcccgac cccgctgctt
     2161 gaccgtatgg aaatcatccg tctgtccggc tataccgaag acgagaaaat caatatcgcg
     2221 atgcagtatc tcgtaccgaa gcaaatgaaa cgcaacggcg tgaaagaagg cgaattggtg
     2281 gtcgaagaaa gcgcggtacg cgatattatc cgttattaca cccgagaggc gggcgtgcgt
     2341 tcgctcgacc gcgaaattgc caaaatctgc cgtaaggtgg tgatgcagat taccttgaac
     2401 gaagataaga agaggttgtc tgaaaccaag aaaaccagca aagccaagcc tagggcggtt
     2461 aaagtcaatg agaaaaacct acatgactac ttgggcgtgc gccgcttcga ttacggcgtt
     2521 gccgaaagtg aaaaccgcat cggacaggtt accggtttgg cttggacgga agtcggcggc
     2581 gaattgctga ctgtcgaagc cgcagcatta ccgggtaaag gcatgatcca gtgtaccggc
     2641 cagttgggcg atgtgatgaa ggaatctgta tccgctgcat ggtctgttgt ccgttcccgt
     2701 gcggaatcag taggtttggc tcctgatttt tacgagaaaa aagacattca tgtccatgtt
     2761 cccgaaggtg caacgccgaa agacggtccg agcgcaggca ttgctatgac tttggcgatg
     2821 gtgtccgcct ttaccaaaat tccggtgcgt gccgatgttg ccatgacggg cgaaattacc
     2881 ctgcgcggcg aagtcctgcc gatcggcggt ctgaaggaaa aactgttggc tgcgcttcgc
     2941 ggcggcatca aacacgtcct gattccgaaa gacaatgtca aagacctgga agaaatccct
     3001 gaaaacgtga aaaccggatt aaccatccat ccggtcaaat ggatagacga ggtattggct
     3061 ttgggtttgg aaagccggcc tgagtcttgg gcagaacctt ctgccgcgga agcggcggcg
     3121 gaatccgctt caaaaccgaa accccgcagc agggcaatca aacattgaaa cgcaggaaat
     3181 gtgttgtaaa aatgcggttt cgtcctgaaa gcctgtaaaa tagggcgatt ccgtattttt
     3241 tgcttgacac ggcaatttca gaattgctat aaagcgaaag ttgctcaagc agtacaaacc
     3301 ccgagtattc gggcatttca tcatttttta agacaggaag ggactgattg tgaacaagtc
     3361 tgaattgatc gaagcgattg ctcaagaagc cgatatttcc aaagccgccg cacaaaaagc
     3421 tttggatgcc actaccaacg cagtaaccaa cgccctgaaa caaggcgaca ccgttactct
     3481 ggtcggtttc ggtactttct acgtcggcga acgtgcggaa cgccaaggcc gcaaccccaa
     3541 aaccggcgag cctctgacca ttgccgccgc caaaacgctt aaattccgtg caggtaaagc
     3601 attgaaagac gcactgtaaa ctgtttttca gcaaaagccg attccttgaa gaatcggctt
     3661 ttttgttttc cccgccgaat caggataggg gtttgcccgt cggattcgga tgaagggctg
     3721 cccgtcaaac ccggatggaa aaatgccatc tgaaacgctt ttccccgcgt tcagacggca
     3781 ttccagacct gaggttcaat cggctttcaa atccgcgaca cattgtttgt ctcgagtttc
     3841 aaacgcgttt gcgccgccga gcaggtcaag ctgttcttgt gcaccgagtt tgccgaagga
     3901 gctgatctgt ttctcgctca atccgtccaa aggctgctcc cacatacatt tgcagtagtc
     3961 gacggcgaga cgggtattgt ccgcatccaa gccgcgcgct ttcaaatcgt tctgccattt
     4021 ttcagcaaac ggaatatttt tcatgcaaga ctcgacaatt ttctgttttg cctgcggttt
     4081 ggacatcgcg cattgggaga gcagggcggt tgaagcgagc aacgccaaaa tgacccacgc
     4141 ccaaatgcgg atggtgcgga ttttggcttt tgcttttttg cgcgcggcaa cctgctcttc
     4201 cgtcggcgtt tcgtgtttcg gctcagtcat gcaggctttc catgcggatc atggtaatcg
     4261 gtttttccac acaatccagt gcttcgatgg ctgcgattgc cgacttgatg tgtttttcga
     4321 ccgtgctgtg ggtcagaatc acgatttcgg cagtggtctg atcgataacg cctttttgga
     4381 ttaaggcttc gatggacacg ttttcttgtg ccaacagcgc ggcgatttgc cccagcgtgc
     4441 ccggttcgtc tttggcttgg acgcgcaggt agtagctgct ggtaatttcg tccataggca
     4501 ggatggtttg cgcttggact tgggcgggtt ggaacgccag atgcggtacg cggtgggcgg
     4561 tgtcggcttc aaccaggcgg gcgatgtcga tgatatcggc aaccacggcg gaagcggtcg
     4621 gcaatgcgcc cgcgcccgcg ccgtaatata aggtttcgcc gaccatatcg gcgttgacgc
     4681 gcacggcgtt catcacgccg tcgacgtttg ccaagaggcg gctttcggga atcagggtcg
     4741 gatggacgcg caattcgatg cctttgccgg ttttgcgggt gatgcccaac agtttgatgc
     4801 ggtagccgag ttcttcggcg tatttgatgt cgcggctgtc gagtttgctg atgccttcga
     4861 gatagcaggc ggagaagttc atcggcgtac cgaatgccag cgcgctcatg atggtgattt
     4921 tatggcctgc gtcgttgcct tcgatgtcga aggtcgggtc ggcttcggca taacccaatg
     4981 cctgcgcttc tttcaatacg tcggcaaacg cgctgccttt ttcgcgcatt tcggagagga
     5041 tgaagttgct ggtgccgtta ataatgccgg cgatggattt aatcctgttt gccgccaaac
     5101 cttcgcgcag ggctttgatg attgggatac cgcccgctac tgccgcttca aattggacga
     5161 tgacgttttg tttttccgcc agcgggaaga tttcgttgcc gtattcggcg agcagttttt
     5221 tgttggcggt aacgatgtgt ttgccgtttt caatggcttt caacaccgct tctttggcaa
     5281 tgcccgtgcc gccgaacaat tcgacgacga catcgacgtc tttacgcgcg accagttcga
     5341 acggatcttt gacaaaggcg gcggacgggc agatttggcg ggctttttct tcgcttaagt
     5401 cgcacatggc agaaatacgg atttcgcgcc ccaagcggcg ggaaatttcc tctgcgttgt
     5461 cccgcaacac ggcagccgta ccgccgccga ccgtacctaa gcctaaaaga ccgatgttta
     5521 ctggcttcat tgtgtctcct tgtaagccga ctgaaatgta aatattgaaa gacgaaaata
     5581 tccgctgccg atataattgt gccgcacttt gaatcaaatg ccgtctgaaa tcggcaggcg
     5641 cgtcagatga aatctgccaa tcctacatta attcgcctga ttttgcaccc ctttcggtgc
     5701 agatgatgca gcaacggggt aaaaaatgtt gtttgaagaa aatccgatag acggacagtt
     5761 tgcagaatat gaatgcggcg cgggcggaat ccggctggcg gggcaaagtt tccataaacc
     5821 cgtgcttgta cataaggatt cggtctgcct gccgcaatgc cgaaccttgt ccgacctgac
     5881 tccggaaaac ctgttgtccg acatcaaacc tgttgactat ccggaaatat tgattatcgg
     5941 gacgggcgcg gctcaggagt ttatccatcc caaaatcatg gcggattttt cccgaatcgg
     6001 aatcagcgtg gaatgcatga ataccgattc ggcattcagg acattggttt tcctgcactc
     6061 ggaagggcgc agggcttggg cttggcttca gccgtaaatt tcccgtttca gacggcatcg
     6121 gcactgactt tcaggtaaaa tacgggcttt tcccgcccga cgatgtttcc gttatgattg
     6181 aaatcaaaaa cctcaccctg caacgcggtt tgaaagtcct gctcgacaag gcttccgctg
     6241 ccgtcaatcc cggtcagcgc gtcggtttga tcggtaagaa cgggacgggc aaatccagcc
     6301 tgtttgcctt aatcaagggc gaaatcactc aggacggcgg cgatatctcg attccgaaaa
     6361 actggcggct cgcttccgtt tcccaagaaa cgcccgattt ggatatttcc gctttggatt
     6421 acgttttgca gggcgatgcc gagttgcagg cttttcagac ggcattgagg caggcggaag
     6481 tgcaaaatga cggcatgaag caggcggaat atcatgccaa attggaagaa atcgacgcct
     6541 ataccgcgcc ggcccgcgcg gcaaaattgt tgaacgggct gggtttttcg caagaagaac
     6601 acagccgtcc cgtcaaatcc ttttccggcg gctggcgtat gcgcctgaat cttgcgcaag
     6661 ccctgatttg ccgcgctgat ttgctcttgc ttgacgaacc gaccaaccat ttggatttgg
     6721 aaactgtctt gtggttggaa aaccaccttg cctctttacc ctgcacgcaa atcatcattt
     6781 cccacgaccg tgatttcctc aatgcggcca ccacccaaac cattgaattg tcgcagcaaa
     6841 agctcacgca atacagcggc aattacgatt tttaccaaac cgaacgcgcg cagcgtctgg
     6901 cgcaacaaca agccgcatat gtcaaacagc aggcgcaaat caaacatctg caatccttta
     6961 tcgaccgctt caaagccaaa gccactaaag ccgttcaagc tcaaagccgc atgaaggcgt
     7021 tggcgaagct cgaacgcatc gctcccgcac atctggacag cgagttttcc tttgagtttt
     7081 acaatcccga ccatctgccc aatcctttgc tgaagctgga gcacgctgat ttgggttacg
     7141 aaggtaaaac cgtcctgcat gacattaccc tgtcgctgga aagcggcgca cgctacgggc
     7201 tattgggtgt caacggcagc ggtaaatcta cgtttatcaa agctttggca ggaaaaatcg
     7261 atttactctc cggcagcatc gtccattccg aaaaacttaa tatcggctat ttcgcccaac
     7321 accagcttga caccatccgc gccgaccaaa gccctgtttg gcatatacag cagctttctc
     7381 ccgaagtacg cgaacaagaa atccgaaatt tcctcggcgg cttcgatttt gtcggcgata
     7441 tggcgttaca gaaaaccgaa cctttttccg gcggagaaaa agcccgcctt gcccttgcca
     7501 tgattatctg gcaaaagccg aacctgctgc tgcttgacga accgaccaac catctggatt
     7561 tggatatgcg tcatgcctta acgctcgcgt tgcaaagttt ccaaggtgca ttaatcgtcg
     7621 tatcgcacga ccgcagcctg cttgaagcca cgaccgacag tttcctcttg atagacaaag
     7681 gtcgtctgaa aaactttgac ggcgatttga acgattaccg ccaatggcgc ttggcacagg
     7741 aaaacgccgc aaccgcgcca gccgcttcca ctcaaagcca aaaccgcaag gataccaagc
     7801 gtatcgaagc gcaaatccgt caagaaaaag cccgacgcag taagccgata caacagaaaa
     7861 tcgacaaagc cgaaaaagaa atggtgcagc tttctgaaat tcagacggga tgcgaagcat
     7921 ttttaacaca agaagacgct taccttgaag caaacaaaga aaaattgcaa aataccttat
     7981 ccgagctggc aaaagtcaaa acacagcttg cccaaatcga agaaacttgg ctggcttgtc
     8041 aagaagaatt ggaacggatt gaaactgaaa tcgagaaaca gtttgccgag cgataaagaa
     8101 gcgcgggcag ggtgcttatc tttgcctgcc aataacggta taattcggac gttaccaccg
     8161 cctttttact ttaccggtat aaacatcaga ctttttgccg ccttgggtct cctgtccctt
     8221 tccggcgcgg cggcgcaagc ctccatatac cattgcaact caaatggcaa aagcgtatat
     8281 acatcagacc cgtcaggatc gtgtgccgat gctgatttac ctaaaatcag cagccatcag
     8341 ggaggcggat accgcctgaa aattaaaaaa cttagtcaag aagccaaaat acacacagga
     8401 aacaaaaaga aaaacaaaaa acatgccggg aaaaagaaca gacaggctgc caaagccccg
     8461 aaggaaaatc aaaaataaac aaccgaaaag aaaagcccat aaaacgccaa gaaaacctta
     8521 caaaaaatcc tcaaaaaatc aaattattat ccgaatatca aacacattat gaaatcaaaa
     8581 ctacccttaa tcctaatcaa cctttccctg atttcaagcc cattgggtgc gaatgcggcc
     8641 aaaatctata cctgcacaat caacggagaa accgtttaca ccaccaagcc gtctaaaagc
     8701 tgccactcaa ccgatttgcc cccaatcggc aactacagca gcgaacgcta tatcccgccc
     8761 caaactcccg aaccggcacc atcaccgtca aacggcggac aggctgtcaa atataaagcc
     8821 ccggtcaaaa cagtatccaa gccggcaaaa tccaatacgc cgcctcaaca agcacctgta
     8881 aataacagca gacgctccat tctcgaagca gaattaagca atgaacgcaa agccctgact
     8941 gaagcccaaa aaatgttatc acaagcacgt ctggcaaaag gcggcaacat caaccatcaa
     9001 aaaatcaacg cattgcaaag caatgttttg gacagacagc aaaatatcca agcactgcaa
     9061 agagaattgg gacgtatgta aggccgtgtt ttcaaatcga ccgttccaag gatttgacag
     9121 aagaaatgat gaaaaagcag gagaattttt gggataagtt gggcgattta ctgtttgcgc
     9181 ccgttgatat aatgttttgg attaaaaaag tatgggcggc atatcctgtg tgtcggctgc
     9241 ctgtaatcgt attgaaggtc aacgtattcc ccaataccgg ctacacgtac aactgtttcc
     9301 gatagttcgc ataatgtata ttatgttaaa tttataatgg attgaataga tatttttaca
     9361 atctcattca aaactcagac acttaaaaaa tttaatcaga acaaataaaa aagcaaacaa
     9421 aaatatccat ctttcaaact catattttgc aataacctag ataagcaaga acaggggcag
     9481 aacggattcg cctttgatgt ggtggtaggc aaccattcca atatgtccca gcgacaaaaa
     9541 cagcaatgaa aatgccagcg tgccgtgaat tcctccgcca ttgccgattt gacgggcaat
     9601 gcctgcggcg gaacagcgag catcagggca tacgcccaat gcccaatttg gtgacggcgg
     9661 tgttggcggg gcggttttta cgttggcgga ttccctaccc tacgcaccaa acagacaata
     9721 tcagcagcag gattctgact gcacggatat gttggtatga atcgtccgcc caagcggaaa
     9781 aggctgtcgc gcccaaaaca gtggcaagaa taaattgctt gaatttcatg atgttgcttc
     9841 ttttgcacaa agcgttcgga atagtgaagc ccgcagccta tggctttgcg gcagtcgcag
     9901 ctttacaagc ggtattgcag gaaaccttaa aacacgatca ttgcatcaag agctgttcga
     9961 attccgccaa atgaacctga cacgcaaact ttgacgcttg aatacggcat tcatagcttc
    10021 cagtttatcg gttcgatacc gtgttgactc aaatagctgt ttgcctgtga aaaatggcgg
    10081 cagccaaaaa aaccgcgata tgccgacaga ggagacggat gaggcgcggt cagtatcaaa
    10141 tgattttgac tgtctatcag cctccctttt tgttgtgcat acccacccca caacatgaag
    10201 acaaggtgct tgcggtgtgt cgcaagctgc ctgatgacgg tatcggtaaa gcgttcccag
    10261 cctaaaaggg cgtgcgaatg cgcctgtcct gcacgcaccg tcaaaaccgt gttcagaagc
    10321 aatatgccct gctccgccca cgctgtcagg cagccgtgcg cgggaatgga aaagccttcg
    10381 atgtcggttt ccaattcttt gtaaatattg agtaaagacg gcggtatgcg gacaccctgc
    10441 cggacggaaa atgccaaacc gtgcgcctgc cctaccccgt gatacggatc ttgtccgaga
    10501 atgacgactt tgacccggtc gaacgctgtc agccggaatg cgttgaacac atcggcctcc
    10561 ggcggataga taatttgccc cgacaaacgt tcctgcctga ccgcatttaa aatttcctga
    10621 aaatacggct gctgtttttc gccgccgagt gcatcgtgcc aagtgtccat atctgcccta
    10681 tcctttcaaa ttcggctata ctgctgatat tgcgttattt tgtcaaacga cgcgctttgt
    10741 ggggaaatgt atgactgtat ggtttgttgc cgctgttgcc gtcttaatca tcgaattatt
    10801 gacgggaacg gtttatcttt tggttgtcag cgcggctttg gcgggttcgg gcattgccta
    10861 cgggctgact ggcagcacgc ctgccgccgt cttgaccgcc gcactgcttt ccgcgctggg
    10921 catttggttc gtacatgcca aaaccgccgt gggaaaagtt gaaacggatt catatcagga
    10981 tttggatatc ggaaaatatg ccgaaatcct ccgatacaca ggcggcaacc gttacgaagt
    11041 tttttatcgc ggtacgcact ggcaggcgca aaatacgggg caggaagtgt ttgaaccggg
    11101 aacgcgcgcc ctcatcgtcc gcaaagaagg taaccttctt atcatcgcaa acccttaaca
    11161 ctcggaggaa ttatggaatt tttcattatc ttgttggcag ccgtcgccgt tttcggcttc
    11221 aaatcctttg tcgtcatccc ccagcaggaa gtccacgttg tcgaaaggct cgggcgtttc
    11281 catcgcgccc tgacggccgg tttgaatatt ttgattccct ttatcgaccg cgtcgcctac
    11341 cgccattcgc tgaaagaaat ccctttagac gtacccagcc aggtctgcat cacgcgcgat
    11401 aatacgcaat tgactgttga cggcatcatc tatttccaag taaccgatcc caaactcgcc
    11461 tcatacggtt cgagcaacta cattatggca attacccagc ttgcccaaac gacgctgcgt
    11521 tccgttatcg ggcgtatgga gttggacaaa acgtttgaag aacgcgacga aatcaacagt
    11581 accgtcgtct ccgccctcga tgaagccgcc ggggcttggg gtgtgaaagt cctccgttac
    11641 gaaatcaagg atttggttcc gccgcaagaa atccttcgcg caatgcaggc acaaattacc
    11701 gccgaacgcg aaaaacgcgc ccgtattgcc gaatccgaag gtcgtaaaat cgaacaaatc
    11761 aaccttgcca gtggtcagcg tgaagccgaa atccaacaat ccgaaggcga ggctcaggct
    11821 gcggtcaatg cgtccaatgc cgagaaaatc gcccgcatca accgcgccaa aggcgaagcg
    11881 gaatccctgc gccttgttgc cgaagccaat gccgaagcca tccgtcaaat tgccgccgcc
    11941 cttcaaaccc aaggcggggc ggatgcggtc aatctgaaga ttgcggaaca atacgtagcc
    12001 gcgttcaaca atcttgccaa agaaagcaat acgctgatta tgcccgccaa tgttgccgac
    12061 atcggcagcc tgatttctgc cggcatgaaa attatcgaca gcagcaaaac cgccaaataa
    12121 gccgtgatga aaataccgtc tgaagctctg ttttcgggtt tcagacggta tttttattta
    12181 aacccgaaca gggttgtatt ccgcttcaaa tatgtttcgg aaccgtctac ctgccccggt
    12241 aaaaactgcc ggcaaaatat ttctgcgcgc cctcgcgaag caagaccaag gttacggcgg
    12301 ccaaaggcaa tccggccaac attccgacaa agcccatcag ctctccgaac gccatcagcg
    12361 aaaagataac ccaaaacggc gacaggccga tacggtctcc tacaattttc ggcgtaatga
    12421 aaaaactttc gagaaactga ccgacggcaa aaaccgccca aacagccaag attccgttcc
    12481 acgaaccgaa ctggagcaag gctgcaacag tggcaagcag caatcccgta aacgcaccca
    12541 aataggggac aaacaccaaa ataccggcaa ccataccgat ggcaaatccc gaatccagtc
    12601 cgactagcat caatcccaaa ccgtaaacca agcccataat cagcatcacc agaagctgac
    12661 cgcgcaaaaa ttcgcccaat acctcgttca aattacccgt aatgcgcgta taagcaccgg
    12721 caaaacgcct cggaaccagt ttggcgatgc cgcacgacca ccgctgccaa tccagcagga
    12781 aatagtaaag cagcaagggc ggcagcagca ggttgccgat actgctgaca atattgccgc
    12841 cctgtttcat caaaacggga aaccacgcct taagcgcgtt gctcaactcg cccgtatgcg
    12901 cctgaagcca cgcaataata gatgcctgat cgatttccac atatccgccg attgtatttt
    12961 tcaaccacgg cagcagcgtg ttctgcataa aaccgattaa ttggggcagg cgagatgcca
    13021 aattattgaa ctgcccgacc agcataggga caataatcaa caataatgcc aacaacaaaa
    13081 tcaaggaaaa caccatcaca gacatcgaag cggatgcacg gttcaaaccc tttttctgca
    13141 accattcgac caaagggtcc aacacatacg ccagcaccgc cgcaaccgca aacggagtca
    13201 aagtatcgcc gagcgcgtaa accagccaga ccaaggcggc aaacgccgcg ccggcaccca
    13261 tccacggctt gatgccccgt ccttttctcc gatacataaa atccaccctt ccgaaaataa
    13321 tattgcgcgc cagtatagca gaaccgccgc gccgtcaaaa aagcctccgc cttcccgata
    13381 gcctgttatc gtggtttagg ctaaaatact gcacatccga tgccctctga aaggcaaacc
    13441 cgccttcaga cggcataccg tacacgataa ggcagcacat catgaccgaa caaaaaaaca
    13501 aagcatccct catcgaattt ccctgcgcct tcccattgaa agtgatgggc gcggtgcatc
    13561 ccgagttcga gcaggcggta ttggacaccg tccgccttca cgcccccgac acgcaggcgc
    13621 accacatcac cacgcgtccg agcagcaaag gcaactatac cggtgccacc gttcaggtaa
    13681 aggttgaaaa ccaagaacaa ttggacaaca tctaccgcgc gctgacttcg cacgaactgg
    13741 tcaaagtggt actttgagat gaaaatcata cacaaaggtt tggtcgaata tctgccgact
    13801 tttgaagcga tgaaaacctt taatgccgga cggaatgcag acaccgaaga cgaattgtgg
    13861 gtcgtcgaac acccgcccgt ctttacgcaa ggactggcgg gaaaacccga gcacctgctg
    13921 attagggacg acatccccgt cgtccaaatc gaccggggcg ggcagattac ctatcacggg
    13981 cccgggcaat tggtcgttta tacgatgatt gatttcaaac ggcgcaaaac cagcgtccgc
    14041 aacatcgttt ccgcgcttga aaacagcatc atcgccacat tggcggaata cggcatcgaa
    14101 gcggcggcag atcccaaacg ccccggcatt tacgtcggcg aacgcaaaat cgcctcactg
    14161 gggctgcgta tcaaaaacgg ttccgtctat cacggactcg cactcaacgt caatatggat
    14221 ttaagcccgt ttacccaaat caacccctgc ggctacgccg gtatggaaat gacgcaaatc
    14281 gcggattttg tccaaccctg ccccgcgccg gacgaagtcg cctccaaact caccgcacac
    14341 cttgaaacac aactcacacc gaaagcagac aacaatgagt gaaatcaaaa ccgacgaccc
    14401 caaacgcggc atcaaactca gaggcgcgga caaaaccgcc cgcatcccca tcaaagtcgt
    14461 cccccttcag gaaaaactga aaaagcccga atggatacgc gccaaactcc catcgcgcaa
    14521 attctttgaa atcaaagaca ttttgcgcga acaaaagatg cacaccgttt gcgaggaagc
    14581 ctcctgcccc aacatcggcg aatgcttcag caaaggcacg gcgaccttca tgattatggg
    14641 cgacatctgc acccgccgct gcccgttctg cgacgtggga cacggccgtc cgaatatgct
    14701 cgaccccgac gagccgaaaa acctcgcaga atccgtcaaa gccatgaacc tgcgttacgt
    14761 cgtcatcacc tcggttgacc gcgacgacct gcgcgacggc ggcgcccaac acttcgccga
    14821 ctgcatcaaa gccatccgcg aaaccagccc gaacaccaaa atcgaaatcc tcgtccccga
    14881 cttccgaggc cgcttggaca tcgcactgaa aatccttgcc gaaaccccgc ccgacgtgat
    14941 gaaccacaac ttggaaaccc atccgagcct gtacagaaaa gcccgtcccg gtgccaatta
    15001 tcaacattct ttagacttat taaaacgtta taaagaaatg atgccgcata tcccgaccaa
    15061 atccggcatt atggtaggct tgggcgaaac agacgaagac gtgcgtgaaa ttatgcgcga
    15121 tatgcgggcg cacaatatcg agatgattac catcggacag tacctccagc cttcagacgg
    15181 acacttgcct gtcttgcgct acgttacacc cgaacaattc aaaatatttg aaaaagaagc
    15241 atacgaactg ggctttacca atgccgccat cggcgcgatg gtacgttcaa gttaccatgc
    15301 cgacgaacag gcggcggaag ccttacgcga aagccacggc ggttgcgggc atcattaaac
    15361 tcggaacacc gttccgaagt aaaaaataat ttcctacgct gctgtctgcc gccttgtttg
    15421 atgtaaacaa catcaatttg aattgtccat gccgtctgaa agtccttcag acggcataat
    15481 ttattcccaa aatccgataa tctcaaaaga atctgtcggt taatgagaaa gattcattat
    15541 aaattttgat aaaaaaccgt gctctatttg ttttttgcta tggttaacaa gtgtaaatgt
    15601 tctttattga tgagatggat tcttagtttt agcagcaaaa aaaaccgata agaaacagca
    15661 tactgatttg gaaggcatcg gggaatacgc cctatttatt ttgataattg tttgtatttt
    15721 aaatagaaaa tttgtcatgc cgacagcttt tgcatacaat gcacgcatcc cgaacagcgt
    15781 tacagaacgc cgtttaaccg cacgaaaccc aaattaagaa aagagagata ttatgcaagg
    15841 caatcaagct gttgttgatt acatgaacga attgctgtcc ggcgagctgg cggcacgcaa
    15901 ccaatacttc atccactccc gcctctactc cgaatggggc tacaccaaac tttttgaacg
    15961 tctcaatcac gagatggaag aagaaaccac acacgccgaa gacttcatcc gacgcatcct
    16021 gatgctgggc ggtacgccga aaatggcacg cgccgaactg aatatcggca cggacgtggt
    16081 ttcctgcctc aaagccgacc tgcaaaccga atacgaagtg cgcgacgctt tgaaaaaagg
    16141 catcaaactg tgcgaagagg ctcaagacta tgttacgcgc gacctgatgg ttgcccaact
    16201 gaaagacacc gaagaagacc acgcacactg gttggaacag cagctgcgcc tgatcgagct
    16261 gattggcgaa ggcaactact accaaagcca actgtaatcc gtattaggaa taaaaggagt
    16321 ccgtatgaaa ggcgaccgtt tggttatccg cgagctgaac aaaaacttag gcttgctgct
    16381 ggtaaccatt aaccaatatt tccttcacgc ccgtattttg aaaaactggg gctttgaaga
    16441 actgggcgaa catttcttca aacaatcaat cgtagaaatg aaagctgccg acgatttgat
    16501 tgaacgcatc ctcttccttg agggattgcc caatttgcaa gagctgggca agcttctgat
    16561 cggtgaatct accgaagaaa tcatcgcctg cgatttgacc aaagaacagg aaaaacacga
    16621 agccctgctt gccgccatcg ccacagcaga agcgcaacag gattatgtca gccgtgattt
    16681 gttggaaaaa caaaaagata ctaacgaaga acacatcgac tggctcgaaa cccagcagga
    16741 actgatcggc aaaatcggtt tgccgaacta cctgcaaaca gcggcgcaag aggactaaaa
    16801 cacaaaccac tgccaaatac agcagtcccc tgcatcatca gacggcatat ccgtataaac
    16861 gggtatgccg tttttctttg ccggtttacg aataaaatct aaattgattt gaatcaatac
    16921 tcgataacgg tttttgtttg ataataccga tatgaaatct agattgtttg tttgctgttt
    16981 cccaatccgt cttgatttta cctctttcct cttgatgtgt gtgtgtttgg gtgtggctgc
    17041 cgccacccct tttttttggc ttttatgtga agtaaaatcc gtaacagcaa atacttaact
    17101 tttaaccatt tatccggaaa caaatgatgg gcaacaaatt gactctgcct gccgaattgc
    17161 ctgacgaaca ggatttgcgg gcggtattgg catacaatat gcggcttttc cgcgtgaaca
    17221 agggttggtc gcaagaggaa ttggcgcggc aatgcggttt ggacaggact tatgtgtcgg
    17281 cagtcgagcg caaacgctgg aacattgccc tgtcgaatat cgaaaaaatg gcggcggctt
    17341 tgggcgtggc ggcgtatcag ttgctgctgc cgccgcagga acggttgaac ctgatgacca
    17401 attccgccga tacccgacaa gtgccgtctg aaagcggtat ttgaccgccg aaccatccgc
    17461 ctcccttttt cccgccaatc gaaagaataa aaatgcctga aaacctgtct tcagcactgg
    17521 aaaccttcaa acagcagcgc aatgccgccg aagcgcatta tttgaaagcc aaccgcgtgt
    17581 cggtattttt cagagaatac acggcggcag tcgaaacctt gctggcggca ttgtgggcag
    17641 aacattttca aaacagcgcg ttatgcctga tggcggtagg cggcttcggg cgcggcgaac
    17701 cgtatccctg ttcggatgtg gatttggcgg ttgtctcccc tgccccgctt tcagacggca
    17761 ttcaggaaca gattgcccga tttatccaaa ctctgtggga ttgcaaacta atgccgtctg
    17821 taaaaagcgg cagcgttgac gaactgtgcg aaagcgtgcg cgacgatatt acgggggaca
    17881 cagcgttttt agaggctagg tttttgtttg gcaaccgcca aacagcagac gaattggcgg
    17941 aaaaaatgaa cgtgcaacgc aatgtagcgg catttatcga agcaaaattg gtggagatgg
    18001 aacaccgcca cgccaaatcg caaggttcgg gggcggtgtt ggagccgaat atcaaaagct
    18061 gtccgggcgg tctgcgcgac atccacaccc tgctttggat agcgaaggcg caaggcttgg
    18121 cggccaacct gcccgacctg ctcaaacagc ggattttgac acgtgccgaa gccggtatgc
    18181 tttcgcacgg ctaccgccgc ctcgcccaca tccgcatcca tctgcattta aacgccaagc
    18241 gcgccgaaga ccgcctgctt ttcgatttgc agccgcaagt tgccgaaagt atgggttatc
    18301 aagatgaaaa ccgccgccgt caaagcgaag aactgatgcg cgtgttttac cgtgcggtca
    18361 aaaccgtcaa acaactgggc ggcatcctca cgcccatgct gcgaagccgc gtttcctcca
    18421 cgccggtgcg cgttaccctg cggattgacg acgactacat ccaagtcaac aaccaaatcg
    18481 ccgcgcggca caccgatatt tttttcagac ggcccgaaca cattttcaaa atcgtcgaaa
    18541 tcatgcagca gcgcaacgac attactgcgc tcgaaccgca aaccctgcgc gcatggtggg
    18601 gggcgacgcg caaaatcaac cgcagcttct accaaaattc tgaaaaccgt cgccgcttcg
    18661 ccggtttttt ccgcagcggc aacggactga cccaaaccct gcgctttctc aacctctacg
    18721 gcgtgttggg ccgctatctg cccgcgtggg aaaaaatcgt cggcctgctt caacacgact
    18781 tgttccacat ctatcccgtg gacgaccaca tccttgccgt cgtccgcaac gtccgccgcc
    18841 ttgccctaga tatgcacagc catgagctgc cctacgcctc cgcgctgatg cagtcctttg
    18901 aaaaacaaga catcctctac cttgccgcct tcttccacga catcgccaaa ggacgcggcg
    18961 gcgaccatgc cgtacaaggc atcgcagacg cgcgccaatt tgccgccgac cacttcctga
    19021 ccgaagaaga aagcgacctg ctcgcctggc tggttgaaaa ccacctcctt atgtctgccg
    19081 tcgcccaaaa agaagacatc caagaccccg gcgtactcga tgccttctgc aaacgcgtcc
    19141 aaacccacga acgcctcagc gcgctctacc tcctgaccat ttccgacata cgcggcacca
    19201 atcccaagct gtggaacgcc tggcgcgcca gcctgctgga aagcctgttc cacgccgccg
    19261 gacgctgcct cgcaggcaac gacggcaacc cgcacgccct cttcggccgc cgccggcagg
    19321 aagccgccga cttacttacc cgcgccgccg tccccgaaaa acagcagaaa aaactatgga
    19381 acgcgctcgg ttccgcctat ttcgcccgcc atcagtcccg cgaaatcctg tggcacgccg
    19441 ccaacctcgt acacgacttt gaacccccca tcgtccgcag ccgcatccta ccccaaagcg
    19501 acagctttca agtgatggtc tttatgccca acggcccgcg cctgttcgcc cgcctctgcc
    19561 gcatcttcag ccgccacggc ttcgatatcc tcgccgcccg cgcctttatc accgaacacg
    19621 actacatcct cgacaccttc atcgtgcaaa tcccctcgca gcacgccccc gaagactacc
    19681 ccgacatcca aagcgcgctc gaagccgaac tcaacagctt tatccacgga cacaccgttg
    19741 ccgaaaccca aagctgcaac cgccgcatca gccgccgcag ccgctatatg cctattgcgc
    19801 caagcatcac catcaccccc gaagaagact atccggaccg gtattccgtc gaaatcaccg
    19861 ccgtcaaccg ccccttcctg ctcgccgaca tggcggaagt cttcttcgcc cacaacgtca
    19921 gcctgcgcta cgccaaaatc tccacgttgg acgaacgcgt cgaagacagc tttaccgtct
    19981 tcagccccga cttgaaaaac ccaaaaatcc agtcctcatt gaaacaggct ttgctggaac
    20041 agttggcata agcaccctgc cggttttcaa acggcatccc gccgccacgc cgtatgccgt
    20101 ctgaaggcgg gggatattgc aaacaatata aactgtcaag ccgccggcaa cgtgctataa
    20161 tgccgtgcat cttgcacaaa ccgaaagacc gagcattatg cgtatcgtag aaaaagccta
    20221 tactttcgac gatgttttgt tggttcccgc acattcgacc gtgctgccgc gagacgttaa
    20281 acttcaaacc aagcttaccc gcgaaatcac actcaacctc cccctgcttt ccgccgcgat
    20341 ggataccgtt accgaggcgc gcctcgccat ttcgatggca caggaaggcg gcatcggcat
    20401 catccataaa aacatgccgc ccgaaatgca ggcgcgcgcc gtttccaaag taaaacgcca
    20461 cgaaagcggc gtggtcaaag accccgtaac cgttgcgccg acaacgctca tccgcgaagt
    20521 cttggaaatg cgcgcgcagc gcaaacgcaa aatgtccggc ctgcccgtcg ttgaaaacgg
    20581 caaagtcgtc ggcatcgtaa ccaaccgcga cctgcgtttt gaaaaccgcg tcgatttgcc
    20641 cgtttccgcc attatgaccc cgcgcgaacg tctggttacc gtccccgaag gcacaagcat
    20701 agacgaagcg cgcgaactga tgcacacgca caaagtcgaa cgcgttttgg tcctgaacga
    20761 aaaagacgag cttaaaggct tgattaccgt taaagatatt ttaaaaacca ccgagtttcc
    20821 caatgccaac aaagactccg aaggccgtct gcgcgtcggt gcggcagtcg gcaccggcgg
    20881 cgacaccgac gagcgcgtca aagccttggt cgaagccggc gcggacgtga ttgtcgtcga
    20941 tactgcccac ggtcatagcc aaggcgtgat cgaccgcgtg cgttgggtca aagaaaccta
    21001 tccgcacatc caagtcatcg gcggcaacat cgccactgcc aaagccgcat tggatttggt
    21061 tgccgtcggc gcggatgccg tcaaagtcgg tatcggtccg ggatcgattt gcaccacccg
    21121 tatcgtggca ggcgtgggcg tgccgcaact gaccgccatt cacaacgttg ccgaagccct
    21181 caaaggcacg ggcgttccgc tgattgccga cggcggcatc cgcttctccg gcgacatcgc
    21241 caaagccctc gccgcaggcg cgtacagcgt catgctcggc ggtatgtttg ccggcacgga
    21301 agaagccccg ggcgaaatcg aactctacca aggccgctcg tacaaatcct atcgcggtat
    21361 gggttccttg ggcgcgatga gccaaggttc tgccgaccgc tacttccaag acaaaaccga
    21421 cagcaccgac aaatacgtcc ccgaaggcat cgaaggccgc gttccctaca aaggtccgat
    21481 tgtgaacatc atccaccaac tgaccggcgg actgcgttcc agtatgggtt atttgggttg
    21541 cgccaatatt gccgaaatgc acgaaaaagc cgaatttgtg gaaatcactt ccgcaggtat
    21601 gagcgaatcg cacgttcacg acgttcaaat taccaaagaa gcgccgaatt accaccgctg
    21661 atttgaacag cctttcagga caaatgccgt ctgaaaccta atttccgagt ttcagacggc
    21721 atttttgttc gttcaaggca agccgcacgg gctgattgcg cggacgggca aagccattct
    21781 gacggacacg ccgaaagaat gaaaatgccg tctgaacgcc gtttgcaccc cgtctgccga
    21841 ataccgtcgg cctttaaaac ggcggtggaa ataaacgcaa tctttcaata cgggcgtttc
    21901 gattggtcgg aaatgatgcg ccggccgcca tat
//
LOCUS       DAXFZR010000031        21722 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000031 DAXFZR010000000
VERSION     DAXFZR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21722
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.031"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..189
                     /locus_tag="ACFJAS_000998"
     CDS             <1..189
                     /locus_tag="ACFJAS_000998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982275.1"
                     /translation="GSVTLYCTRRAVVVIFYLIVFKQNKNFRYVNEFSCPDLTECLKF
                     LIVSIGGELWGKVSLLPL"
     gene            215..670
                     /gene="mraZ"
                     /locus_tag="ACFJAS_000999"
     CDS             215..670
                     /gene="mraZ"
                     /locus_tag="ACFJAS_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC3982276.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            667..1623
                     /gene="rsmH"
                     /locus_tag="ACFJAS_001000"
     CDS             667..1623
                     /gene="rsmH"
                     /locus_tag="ACFJAS_001000"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC3982277.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            1650..1913
                     /gene="ftsL"
                     /locus_tag="ACFJAS_001001"
     CDS             1650..1913
                     /gene="ftsL"
                     /locus_tag="ACFJAS_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC3982278.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            1974..3722
                     /locus_tag="ACFJAS_001002"
     CDS             1974..3722
                     /locus_tag="ACFJAS_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC3982279.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGLSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSF
                     EKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNG
                     RYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTAHSYYGGVVAGPPFKKIMGGSLNILG
                     ISPTKPLTAAAVKTPS"
     gene            3747..5225
                     /locus_tag="ACFJAS_001003"
     CDS             3747..5225
                     /locus_tag="ACFJAS_001003"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC3982280.1"
                     /translation="MFSKLSPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGRFAWNPEWKVPNQGIKDLKHRAGMLAAQVYDN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGMEAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            5248..6894
                     /locus_tag="ACFJAS_001004"
     CDS             5248..6894
                     /locus_tag="ACFJAS_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC3982281.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            7019..8377
                     /gene="murF"
                     /locus_tag="ACFJAS_001005"
     CDS             7019..8377
                     /gene="murF"
                     /locus_tag="ACFJAS_001005"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC3982282.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            8380..8556
                     /locus_tag="ACFJAS_001006"
     CDS             8380..8556
                     /locus_tag="ACFJAS_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982283.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            8801..9883
                     /gene="mraY"
                     /locus_tag="ACFJAS_001007"
     CDS             8801..9883
                     /gene="mraY"
                     /locus_tag="ACFJAS_001007"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC3982284.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            10008..10388
                     /locus_tag="ACFJAS_001008"
     CDS             10008..10388
                     /locus_tag="ACFJAS_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC3982285.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            10473..11810
                     /gene="murD"
                     /locus_tag="ACFJAS_001009"
     CDS             10473..11810
                     /gene="murD"
                     /locus_tag="ACFJAS_001009"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC3982286.1"
                     /translation="MTFQNKKTLVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            11989..13260
                     /locus_tag="ACFJAS_001010"
     CDS             11989..13260
                     /locus_tag="ACFJAS_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC3982287.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            13264..14331
                     /gene="murG"
                     /locus_tag="ACFJAS_001011"
     CDS             13264..14331
                     /gene="murG"
                     /locus_tag="ACFJAS_001011"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC3982288.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            14486..15895
                     /gene="murC"
                     /locus_tag="ACFJAS_001012"
     CDS             14486..15895
                     /gene="murC"
                     /locus_tag="ACFJAS_001012"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC3982289.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKILNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            16007..16921
                     /locus_tag="ACFJAS_001013"
     CDS             16007..16921
                     /locus_tag="ACFJAS_001013"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC3982290.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            16911..17639
                     /locus_tag="ACFJAS_001014"
     CDS             16911..17639
                     /locus_tag="ACFJAS_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC3982291.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            17725..18969
                     /gene="ftsA"
                     /locus_tag="ACFJAS_001015"
     CDS             17725..18969
                     /gene="ftsA"
                     /locus_tag="ACFJAS_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC3982292.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            19088..20266
                     /gene="ftsZ"
                     /locus_tag="ACFJAS_001016"
     CDS             19088..20266
                     /gene="ftsZ"
                     /locus_tag="ACFJAS_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC3982293.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLREHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            20402..21712
                     /locus_tag="ACFJAS_001017"
     CDS             20402..21712
                     /locus_tag="ACFJAS_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC3982294.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
BASE COUNT         4956 a         5441 c         6247 g         5078 t
ORIGIN      
        1 ggttcggtca ctctgtactg cacccgccgt gccgttgtcg tcatttttta tcttattgtt
       61 tttaaacaga ataaaaattt cagatatgtt aatgagtttt catgccctga tttgaccgag
      121 tgtttaaaat ttcttatagt gtcgattggt ggggaattgt ggggcaaagt gtctctttta
      181 cccttgtgat tttgatttcg gcttggggca tgtcatgttc ggcggcgcac acgaattaag
      241 catcgacagt aaggggcggt tggctgttcc tgccaaattc cgcgacattc tgtcgcgcct
      301 ctatacgcct gccgtagtgg caacgctcga gtcgaaacat aagctgttga tgtaccctgt
      361 tgcggagtgg gaaaaggttg cggcgcaact tttaaactta aaagtggcgg ataaccctgt
      421 tttgcggcgg tttcaaaatc ttttgctgca taacgcggaa attttggaat gggacagcgc
      481 cggcagggtg ctggttcctg ccggactgag gaaaagggtg gatttcgacc gtgaagttgt
      541 tttggtcggc cgtgccaacc gtttggagct ttggggtcgc gagcagtggg aggctgagat
      601 ggttcaggct ttggatgacg atcctgacga acttgccttc cagttgagtc agacggattt
      661 gcaattgtga gtggagcaga aagttaccgg catatcacgg tcttgctgaa tgaggcggtg
      721 gatgcgcttg ccgtgcgcga agacggtgtc tatgtggacg gtacgttcgg caggggaggg
      781 cattcccggc tgattttgtc gcgtttgggc gatgcggggc ggttgattgt tttcgacaaa
      841 gacccgcagg cgattgctgt ggcagaagag ctggcgcgtt cggacaaacg ggtcggtgtc
      901 gtgcatggcg gttttgcttc gtttcagacg gcattggacg gtttgggtat cggcaaggtg
      961 gacggtgcgc tgtttgattt ggggatttcg tctccgcaaa tcgatgacgg cagccgcggt
     1021 ttcagcttcc gtttcgatgc ccctttggat atgcgtatgg atacgacgcg cggtatgtct
     1081 gccgcagagt ggatagcggt tgcgtcggaa caggatttgc acgaggtaat caagaattat
     1141 ggtgaagagc ggtttagccg ccagattgtg cgcgccattg tcgcgcaacg ggcggaaagt
     1201 ccaatcgata caacccgcaa gctggcgcag atcgtggcac aaaacgtccg tactcgcgag
     1261 cgggggcagg atcctgcgac gcgcaccttc caggcaatcc gcatctttat taaccgcgag
     1321 cttgaagaag taggggcggt attgccgcag gtcatgtgcc gtctgaaaga gggcgggcgt
     1381 ttggcggtca ttgctttcca ttcgttggaa gaccgcattg tgaagcagtt tgtcaaaaaa
     1441 tattcgcaac acgagcccct gccgagctgg gcggcggtca gagaagcgga tttgcccgag
     1501 ccgcccctga aaatcgtggg cagggcatta aagccgggtg aagcggaaat tgccgccaac
     1561 ccgagggcga ggagcgcagt tttgcgtgtg gcggagcgga ctgccggtcc gataccggaa
     1621 taataacaga gaaaaacgtc tgaacggcaa tgaacaaatc gaatttcttt ctgctgcttg
     1681 cggtgtgcgt ttccgctttt tccgttgtga tgcagcaaaa ccagtacagg ctcaacttca
     1741 cagctttgga caaggcgaaa aaacaggaaa tcgccttgga gcaggattat gcgcaaatga
     1801 gggtgcaaca ggcgcgtttg gcgaatcacg aagcgatcag ggcggcggca gaaaaacaaa
     1861 acctccatcc gccggtttcg ggcaatacct ttatggtgga acatcaaaga tagaagcagc
     1921 ctgtgtgccg gaatcggatt cctgcatcag gataataata acgagaagta aaaatgttga
     1981 ttaaaagcga atataagccc cggatgctgc ccaaagaaga gcaggtcaaa aagccgatga
     2041 ccagtaacgg acggattagc ttcgtcctga tggcaatggc ggtcttgttt gcctgtctga
     2101 ttgcccgcgg gctgtatctg cagacggtaa cgtataactt tttgaaagaa cagggcgaca
     2161 accggattgt gcggactcaa gcattgccgg ctacacgcgg tacggtttcg gaccggaacg
     2221 gtgcggtttt ggcgttgagc gcgccgacgg agtccctgtt tgccgtgcct aaagatatga
     2281 aggaaatgcc gtctgccgcc caattggaac gcctgtccga gcttgtcgat gtgccggtcg
     2341 atgttttgag gaacaaactc gaacagaaag gcaagtcgtt tatttggatc aagcggcagc
     2401 tcgatcccaa ggttgccgaa gaggtcaaag ccttgggttt ggaaaacttt gtatttgaaa
     2461 aagaattaaa acgccattac ccgatgggca acctgtttgc acacgtcatc ggatttaccg
     2521 atattgacgg caaaggtcag gaaggtttgg aactttcgct tgaagacagc ctgtatggcg
     2581 aagacggcgc ggaagttgtt ttgcgggacc ggcagggcaa tattgtggac agcttggact
     2641 ccccgcgcaa taaagcaccg caaaacggca aagacatcat cctttccctc gatcagagga
     2701 ttcagacctt ggcctatgaa gagttgaaca aggcggtcga ataccatcag gcaaaagccg
     2761 gaacggtggt ggttttggat gcccgcacgg gggaaatcct cgccttggcc aatacgcccg
     2821 cctacgatcc caacagaccc ggccgggcag acagcgaaca gcggcgcaac cgtgccgtaa
     2881 ccgatatgat cgaacctggt tcggcaatca aaccgttcgt gattgcgaag gcattggatg
     2941 cgggcaaaac cgatttgaac gaacggctga atacgcagcc ttataaaatc ggactgtctc
     3001 ccgtgcgcga cgatacccat gtttacccct ctttggatgt gcgcggcatt atgcagaaat
     3061 cgtccaacgt cggcacaagc aaactgtctg cgcgtttcgg cgccgaagaa atgtatgact
     3121 tctatcatga attgggcatc ggtgtgcgta tgcactcggg ctttccgggg gaaactgcag
     3181 gtttgttgag aaattggcgc aggtggcggc ccatcgaaca ggcgacgatg tctttcggtt
     3241 acggcctgca attgagcctg ctgcaattgg cgcgcgccta taccgcactg acgcacgacg
     3301 gcgttttgct gccgctcagc tttgagaagc aggcggttgc gccgcaaggc aaacgcatat
     3361 tcaaagaatc gaccgcgcgc gaggtacgca atctgatggt ttccgtaacc gagccgggcg
     3421 gcaccggtac ggcgggtgcg gtggacggtt tcgatgtcgg cgctaaaacc ggtacggcgc
     3481 gcaaactggt caatggccgc tatgtggaca acaaacacgt cggtacgttt atcggttttg
     3541 cccccgccaa aaacccccgt gtgattgtgg cggtaaccat cgacgaaccg actgcccaca
     3601 gctattacgg cggcgtagtg gcagggccgc ccttcaaaaa aattatgggc ggcagcctga
     3661 acatcttggg catttccccg accaagccac tgaccgccgc agccgtcaaa acaccgtctt
     3721 aatccgagta tcaacgagat tgttttatgt tcagcaagtt aagccctttg gctgaaaccg
     3781 gcatcccgac cctgtcgtgt gcaaacgcgg cagggcgttt gttgcattca gacagccgcc
     3841 aaatcaaaca aggcgatatt ttcgttgcct gtccgggcga atatgccgac ggccgcagtt
     3901 atatccccgc cgccgttgcc aacggcgcgg cttttgtttt ttgggacgac gacggcagat
     3961 tcgcgtggaa tcccgaatgg aaagtcccca atcaaggcat caaagatttg aaacaccgtg
     4021 ccggcatgtt ggcggcgcaa gtgtacgaca acgtttcaga cggcctcaaa gtctggggcg
     4081 taaccggcac caacggcaaa acctccatca cacaatggct ggcgcaggct gccgatttat
     4141 tgggcgaaaa aaccgccatt atcggcacgg tcggcaacgg cttttggggc gcattggaag
     4201 aaaccacgca caccaccccc gcccccgtcg atgtccaaac cctgctctac cgtttccgcc
     4261 aacagggcgc aacagccgcc gcgatggaag tctccagcca cggtcttgac cagtcgcgcg
     4321 tcaacggcgt gccattccgc agcgcaatct ttaccaacct cacccgagac cacctcgact
     4381 accacggcac gatggaagcc tacggtgcta tcaagtcgcg cctgttttac tggcacggct
     4441 tgaaacacgc cgttatcaat accgatgacg gatacggcgc ggaactcgca ggtcgtctga
     4501 aaaaagactg tcccgattta gccgtttaca gctatggttt cagcgaacac gccgacatcc
     4561 gcattaccga ctttaccgcc tcttcagacg gcatggaagc agtattccaa accccgtggg
     4621 gcgaaggaaa atgccgcacc cgcctgctcg gacggttcaa cgcgcaaaac ctcgccgcct
     4681 gcatcgcctt gctttgtgcc aacggttatc cgcttgataa ggtattggat gtgctggcaa
     4741 aaatccgtcc cgcttcaggg cgcatggact gcatcatgaa cagcggcaag cccttggtcg
     4801 tcgtcgatta cgcccacacg cccgacgcat tggaaaaagc actctccact ttgcaggaaa
     4861 tcaaaccgca gggtgcggcc ttatggtgcg tattcggttg cggcggcaac cgcgattgcg
     4921 gcaaacgccc attgatgggc gcggcagccg tacagggcgc ggataaagtc gtcgtaacca
     4981 gcgacaaccc gcgtttggaa aatccgcaag acatcatcaa cgacatcctg cccgccgtgc
     5041 ccgcgcccga atgcgtcgaa gccgaccgtg ccgccgccat ccgttatgcg gttgagcagg
     5101 ccgccgcaaa cgacatcatc ctgattgccg gcaaaggaca cgaaaactat caggatgtac
     5161 agggcgtgaa gcaccgtttt tccgatcttg aaatcgtcgg gcaggctttg ttaactcgta
     5221 aataatggga tattcggacg gcatcgtatg aaacaatccg cccgaataaa aaatatggat
     5281 cagacattaa aaaatacatt gggcatttgc gcgcttttag ccttttgttt tggcgcggcc
     5341 atcgcatcag gttatcactt ggaatatgaa tacggctacc gttattctgc cgtgggcgct
     5401 ttggcttcgg ttgtattttt attattattg gcacgcggct tcccgcgcgt ttcttcagtt
     5461 gttttactga tttacgtcgg cacaaccgcc ctatatttgc cggtcggctg gctgtatggt
     5521 gcgccttctt atcagatagt cggttcgata ttggaaagca atcctgccga ggcgcgtgaa
     5581 tttgtcggca atcttcccgg gtcgctttat tttgtgcagg cattattttt catttttggc
     5641 ttgacagttt ggaaatattg tgtatctgtg ggggtatttg ctgacgtaaa aaactataaa
     5701 cgccgcagca aaatatggct gaccatatta ttgactttga ttttatcctg cgcggtgatg
     5761 gagaaaatcg ccggcgataa agattggcga gaacctgatg ccggcctgtt gttgaatatt
     5821 ttcgacctgt attacgattt ggcttccgcg ccggcacaat atgccgccaa gcgcgcccac
     5881 attttggaag cagcaaaaaa agcgtcaaca tggcatatcc gtcatgttgc gcccaagtat
     5941 aaaaattatg ttgtggttat cggtgagagc gcgcgttcgg attatatgaa tgtttacggt
     6001 ttcccattgc ccgatacgcc ttttttgagt cggaccaaag ggctgttgat aaacggttac
     6061 caatcgaccg cccacgcgac gaatctttcg ctgccgcaga ctttggggct gccgggagaa
     6121 ccgaacaata acattgtcag cttggcgaag caggctggtt ttcggacggc gtggctgtct
     6181 aatcaaggaa tgttggggca ttttgccaac gaaatttcca cctatgccct acgcagcgat
     6241 tatccgtggt ttacccaaag gggtgattat ggcaaaagcg cgggattgag cgaccgcctt
     6301 ttgttgccgg cgttcaaacg ggttttgaca ggaaatgcag gcacgaagcc tcggctgatt
     6361 gtgatgcacc tgatgggttc gcacagtgat ttttgcacac gtttggataa ggatgcgcgg
     6421 cggtttcagt atcaaactga aaaaatatcc tgctatgttt ccaccatcgc gcaaaccgat
     6481 aaatttttag aagatacggt taagatattg aatgaaaata aagaaagctg gtctttggtt
     6541 tacttttccg accacggttt gatgcatgtc ggcaaaggcg gcgagcgaac gttgacacac
     6601 ggcgagtgga agcgtcaaag ctacggcgtg ccgctggtta aaatttcgtc cgatgacacg
     6661 cggcgcgaaa tgattaaagt gaggcgcagc gcgtttaatt ttttacgcgg attcggcagt
     6721 tggacgggta tcgaaaccga cgagttgccc gatgacggct atgatttttg ggggaatgtc
     6781 cccgatgtgc cgggcgaagg caataacctt gcatttatcg acagacagtc cgacgacccc
     6841 gcgccgtggt atgcgggaaa aggcaaatcg gctaaaaata cgtctaaaaa atgatacgta
     6901 cagaaaaaat gccgaatgag aatgggaaaa taatctgtgt tttaccacag caaaacaggc
     6961 gataaaaaaa tcagccgcta ccgatgtgtc cgccgcccga atattaacga aagcaaatat
     7021 gaaaccattg gacctaaatt tcatctgcca agccctcaag cttccgatgc cgtctgaaaa
     7081 caaacccgtg tcgcgcatcg taaccgacag ccgcgatatt cgggaaggcg atgtgttttt
     7141 cgcattggcg ggcgggcggt ttgacgcgca tgattttgtt ggaggcgtat tgtctgcggg
     7201 cgcggcggcg gttgtggttt cgcgcgaaga ttgcgcggct ttgggcggcg cgttgaaagt
     7261 cgatgacacg cttgccgcgt tgcaaacgtt ggcgaaggcg tggcgcgata atgtgaaccc
     7321 gtttgtgttc ggcattaccg gttcgggcgg caagacgacg gtgaaggaga tgctggctgc
     7381 ggtattgcgc cgccgtttcg gcgatgatgc cgtttcggcg acggcaggca acttcaacaa
     7441 ccacatcgga ttgccgctga ctttattgaa attaaacgaa aaacaccgct atgccgtgat
     7501 tgaaatgggc atgaaccatt ttggcgaact ggcggtttta acgcaaatcg ccaaacccga
     7561 tgccgctttg gtcaacaacg ccctgcgcgc ccatgtcgga tgcggtttcg acggagtggg
     7621 cgatattgcc aaagcgaaaa gcgagattta tgcaggctta tgttcagacg gcatggcact
     7681 gattcctcaa gaagatgcca atatggctgt cttcaaaacg gcaacgttta atttgaatac
     7741 gtgcactttc ggcgtcgata gcggcgatgt ccgcgcggaa aatatcgtgc tgaaaccttt
     7801 gtcgtgcgaa tttgatttgg tgtgcggcga cgagcgcact gccgtggtgc tgcctgttcc
     7861 cggccgccac aatgtccaca acgccgccgc tgccgccgcg ctggctttgg ctgccggttt
     7921 gagtttgaac gatgtggcgg aaggtttgca aggcttcagc aacatcaaag gccgtctgaa
     7981 cgtcaaagcc ggcatcaagg gcgcaaccct gattgacgat acttataatg cgaatcccga
     8041 cagtatgaaa gccgcggttg acgtgttggc gcgtatgcct gcgccgcgca ttttcgtgat
     8101 gggcgatatg ggcgaactgg gcgaggacga agccgccgcc atgcacgccg aagtcggcgc
     8161 gtacgcccgc gaccaaggca tcgaagcggc ttattttgtc ggcgacaaca gcgtcgaagc
     8221 ggcggaaaaa tttggcgcgg acggtttgtg gttcgccgcc aaagacccgt tgattcaagt
     8281 gttgagccac gatttgcccg aacgcgccac cgtgttggtg aaaggttcgc gctttatgca
     8341 gatggaagaa gtggtcgagg cattggagga taagtgaaaa tgaaaagccg acgttttttt
     8401 aaagccttat tgctgattgc cgcgctggtc ggcgcgtttt atgccggaat gcggacgcag
     8461 gcgtatcttt atgaagattt atgtttagac ttgggcggcg gcaaaaatcc ggggagctac
     8521 ccgatttgcg tgattgggaa agtccctgca cgttaatctg caaaagccgt ccgaaacctt
     8581 gccggacagc aagcaaacct caagcgggcg caagcccgat gtatagcgga ttaacaaaaa
     8641 tcaggacaag gcggcgggcc gcagacagta cggatagtac ggaaccgatc cgcccggtgc
     8701 ttgggcgcct tagggaaccg ttccctttga gccggggcgg ggcaacgacg taccggtttt
     8761 tgttaatccg ctataacgac aaaacaaaaa aggaagcccc atgtttttat ggctcgcaca
     8821 tttcagcaac tggttaaccg gtctgaatat ttttcaatac accacattcc gcgccgttat
     8881 ggcggcgttg accgccttgg cgttttccct gatgttcggc ccgtggacga tacgcaggct
     8941 gaccgcgctc aaatgcgggc aggcagtgcg taccgacggc ccgcaaaccc acctcgtcaa
     9001 aaacggcacg ccgacgatgg gcggttcgct gattctgacc gccattaccg tgtccaccct
     9061 gttgtggggc aactgggcga acccgtatat ctggattctc ttgggcgtac tgcttgccac
     9121 cggtgcgctc ggtttttacg acgactggcg caaagtcgtt tataaagacc ccaacggcgt
     9181 gtccgccaaa ttcaaaatgg tgtggcagtc aagcgttgcc gttatcgccg gtttggcatt
     9241 gttttacctt gccgccaatt ccgccaacaa tattttgatt gtcccgtttt tcaaacaaat
     9301 cgccctgccg ctgggcgtgg tcggcttttt ggtgttgtct tacctgacca tcgtcggcac
     9361 atccaacgcc gtcaacctca ccgacggctt ggacggcctt gccgccttcc ccgtcgtcct
     9421 cgttgccgcc gggctcgcca ttttcgccta cgtcagcgga cactaccaat tttcccaata
     9481 cctccagctt ccctatgtcg ccggcgcgaa cgaagtcgct atattctgca ccgccatgtg
     9541 cggcgcgtgc ctcggatttt tgtggttcaa cgcctatccc gcgcaagtct ttatgggcga
     9601 tgtcggcgcg ctggcattgg gtgccgcgct cggtaccgtt gccgtcatcg ttcgccaaga
     9661 atttgtcctc gtcattatgg gcggtctgtt cgtcgtagaa gccgtgtccg ttatgcttca
     9721 ggtcggctgg tacaagaaaa ccaaaaaacg catcttcctg atggcgccga ttcatcacca
     9781 ttacgaacaa aaaggctgga aagaaaccca agtcgtcgtc cgtttctgga ttattaccat
     9841 cgtcttggta ttgatcggtt tgagtaccct caaaattcgc tgaacctatg ccgtccgaac
     9901 acctttcaga cggcatttga acgcgcaata aacctgcggc ggcaatccgc cccgtcctat
     9961 cgttaacggc ggctgaaacc cgccttatac taaaacagga gaaaaccatg aaacagacag
    10021 tcaaatggct tgccgccgcc ctgattgcct tgggcttgaa ccaagcggtg tgggcgggtg
    10081 acgtatcgga ttttcgggaa aacttgcagg cggcagaaca gggaaatgca gcagcccaat
    10141 tcaatttggg cgtgatgtat gaaaatggac aaggagttcg tcaagattat gtacaggcag
    10201 tgcagtggta tcgcaaggct tcagaacaag gggatgccca agcccaatac aatttgggct
    10261 tgatgtatta cgatggacgc ggcgtgcgcc aagaccttgc gctcgctcaa caatggcttg
    10321 gcaaggcttg tcaaaacgga gaccaaaaca gctgcgacaa tgaccaacgc ctgaaggcgg
    10381 gttattaatc ggctcgcgat gccgtctgaa agcggcttgg gcaagggcgg acgtctcctg
    10441 cccaatatga tttgttttag gacaaaacca aaatgacttt ccaaaacaaa aaaaccctcg
    10501 tcgccggact tggcggcacg ggtatttcca tgattgccta tctgcgcaaa aacggcgcgg
    10561 aggttgccgc ttatgatgcg gagctgaaag ccgagcgcgt ggcgcaaatc ggtaagatgt
    10621 ttgacgggct ggtgttttac acgggtcgtc tgaaagatgc gttggacaac ggtttcgata
    10681 ttctggcgct cagtcccggc atcagcgagc ggcagccgga tatcgaggcg ttcaagcaaa
    10741 acggcgggcg cgtgctaggc gacatcgaat tgctggcgga cattgtgaac cgccgcggcg
    10801 acaaggtgat tgcgattacc ggcagcaacg gcaaaaccac ggtaacgagc ctggtcggct
    10861 atctctgcat caagtgcggg ctggataccg tcattgcggg caatatcggt acgccggttt
    10921 tggaggcaga attgcagcgc gaaggcaaaa aggcggacgt gtgggtgttg gagctttcca
    10981 gcttccaact ggaaaacacc gaaagcctgc gcccgactgc ggcgacggtg atgaacattt
    11041 ccgaagacca tctcgaccgc tacgacgatt tgctcgacta cgcgcatacc aaagccaaga
    11101 ttttccgtgg cgatggagtg caggttttga atgcggacga cgtgttctgc cgcgccatga
    11161 aacgggcagg gcgtgaagtg aaacggtttt ccttggaaca cgaagccgat ttttggttgg
    11221 aacgcgggac gggctgttta aaacaaggca atgaagattt gatttctacg caagacatcc
    11281 cgctgcaagg tttgcacaac gccgccaacg ttatggctgc cgtggctttg tgcgaggccg
    11341 tcggtttgcc gcgcgaagca ttgctggaac acgtcaaaac cttccaaggc ttgccgcacc
    11401 gtgtggaaaa aatcggcgag aaaaacggcg tggtattcat cgacgacagc aaaggcacga
    11461 atgtcggcgc gaccgccgcc gcgattgccg gtttgcaaaa cccgctcttc gtgattttgg
    11521 gcggcatggg caaagggcag gacttcacgc ccctgcgcga cgcgttgaaa gataaggcaa
    11581 aaggcgtgtt cctgatcggc gtcgatgcgc cgcaaatccg ccgcgatttg gacggctgcg
    11641 gcttgaacct gaccgactgc gtcactttgg aagaggcggt tcagacggca tacgcccaag
    11701 ccgaagcggg cgatattgtc ttgctcagcc ccgcctgcgc gagtttcgat atgtttaaag
    11761 gctacgcgca ccgttcggaa gtgtttatcg aagcgtttaa ggctttgtga tgccgtccga
    11821 aacacaaacg ccgtcattgt tgggcggcaa gtaaagattt agaataccga tttgggatgt
    11881 atcgtatgtt cggacggcat tgtctgccgt ctgaaatttt tgccctttgc ggcaggtgca
    11941 aacagactgg caggtggttt tttgaagatt tcggaagtat tggtaaaagt gggcgacggt
    12001 gtccacactc tgctgctcga caggccgatt gtgcgcgacg gcaggaaatt cgacgcgccg
    12061 cttttgtgga tggtggtgct gatgacggcg ttcggcctgc tgatgattta ttcggcttct
    12121 gtgtatttgg catcgaagga aggcggcgat cagtttttct atttgaccag gcaggcgggg
    12181 ttcgtcgttg ccggccttat agcgagcggt tttttatggt ttctttgcag gatgaggaca
    12241 tggcggcggc ttgtgccgtg gatttttgcc ttatccggcc tgttgctggt agccgtattg
    12301 attgccgggc gcgaaatcaa tggcgcgacc cgttggatac ctttgggtcc gttgaatttc
    12361 cagccgaccg agctgttcaa gctggcagtc atcctttatt tggcaagcct gttcacgcgc
    12421 cgtgaagaag tgttgcgcag catggaaagt ttgggttggc agtcgatttg gcgggggacg
    12481 gccaacctga ttatgtccgc caccaatccg caggcacgtc gtgaaacatt agaaatgtac
    12541 ggccgtttcc gggcgatcat cctgccgatt atgctggtgg cgttcggttt ggtgctgata
    12601 atggtacagc cggatttcgg ttcgtttgtc gtcattaccg tcattaccgt tggaatgctg
    12661 tttctggcag gattgccgtg gaaatatttt tttgtcctgg taggcagcgt cttgggtggg
    12721 atggtgctga tgattaccgc cgctccctac cgtgtgcagc gggtagtggc atttttggac
    12781 ccgtggaaag acccgcaggg tgccggctac cagcttaccc actctctgat ggcaatcggg
    12841 cgcggagagt ggttcggtat gggtttgggt gcgagtttga gcaaacgcgg ctttctgccg
    12901 gaagcgcata ccgattttat ttttgccatc atcgctgaag aattcggctt cttcgggatg
    12961 tgcgtgctga tattctgtta cggctggctg gtggtgcggg cgttttccat cggcaagcag
    13021 tcgcgcgatt tgggtttgac tttcaacgcc tatatcgctt cgggtatcgg catttggatc
    13081 ggtatccaaa gtttcttcaa tatcggtgtg aacatcggtg ctttgccgac caaaggtctg
    13141 acgctgccgt tgatgtccta tggcggttcg tcagtctttt tcatgctgat cagcatgatg
    13201 ctgctgttgc gtatcgatta tgaaaaccgc cggaaaatgc gcggttaccg ggtggagtaa
    13261 atcatgggcg gtaaaacctt tatgctgatg gcgggcggaa cgggcggaca cattttccca
    13321 gctctggctg tggcggattc attgcgcgtg cgcggtcatc atgtaatttg gctgggcagc
    13381 aaggattcga tggaagagcg catcgtgccg caatacggca tacgcttgga aacgctggcg
    13441 attaaaggaa tacgcggcaa cggcatcaaa cgcaagctga tgcttccgtt tactctgtac
    13501 aaaaccgtcc gcgaagcgca gcggattatc cgcaaacacc gtgtcgagtg cgtcatcggc
    13561 ttcggcggtt ttgttacctt tcccggcggt ctggcggcga aactcttggg cgtgccgatt
    13621 gtgattcacg agcaaaacgc cgtggcaggc ttgtccaacc gccacctgtc gcgctgggcg
    13681 aaacgggtgt tgtacgcttt tccgaaagcg ttcagccacg aaggcggttt ggtcggcaac
    13741 cccgtccgcg ccgatattag caacctgccc gtgcctgccg aacgcttcca agggcgcgaa
    13801 ggccgtctga aaattttggt ggtcggcggc agtttgggtg cggacgtttt gaacaaaacc
    13861 gtaccgcagg cgttggcact gctgcctgaa gaggtgcgcc cgcagatgta ccaccagtcg
    13921 gggcgtaaca agctgggcaa tcttcaggcg gattatgacg cgttgggcgt gaaagcggaa
    13981 tgcgtggaat ttattaccga catggtgtcc gcctaccgtg atgccgattt ggtgatttgc
    14041 cgtgccggcg cgctgacgat tgccgagttg acggcggcgg ggctgggcgc gttgttagtg
    14101 ccgtatcctc acgccgttga tgaccatcaa accgccaacg cgcgtttcat ggtgcaggca
    14161 gaagcggggc tgctgttgcc gcaaacccag ttgacggcgg aaaaactcgc cgaaatcctc
    14221 ggcagcctca accgcgaaaa atgcctcaaa tgggcggaaa acgcccgtac gttggcattg
    14281 ccgcacagcg cggatgacgt tgccgaagcc gcgattgcgt gtgcggcgta aactgccgaa
    14341 ccatgccgtc tgaaaagccg ttcagacggc atggatgttt tttatttcaa tccgctatat
    14401 atttgtcaga aaactatggt gcgcaaacgg tcagaccttt aaaataacgc ctttacgcac
    14461 taaaaaccaa ccggaacgca acattatgat gaaaaatcga gtaagcaaca tccattttgt
    14521 cggtatcggc ggcgtcggca tgagcggtat cgccgaagtc ttgcacaatt tgggctttaa
    14581 agtttccggt tcggatcagg cgcgaaatgc cgctaccgag catttgagca gcctgggcat
    14641 tcaagtttat cccggccata ccgcagaaca cgttaacggt gcggatgtcg tcgttgcctc
    14701 taccgccgtc aagaaagaaa atcccgaagt tgtcgctgcg ttggagcggc aaattcccgt
    14761 tattccgcgc gccttgatgc tggcagagct gatgcgcttc cgtgacggca tcgccattgc
    14821 cggtacgcac ggcaaaacca cgaccaccag cctgaccgcc tccatcctcg gcgcggcagg
    14881 actcgacccc actttcgtta tcggcggcaa actcaacgcc gcaggcacca acgcccgctt
    14941 gggcaaaggc gaatacatcg ttgccgaagc cgacgaatcc gatgcctctt tcctacatct
    15001 gaccccgatt atgtccgtcg ttaccaatat cgacgaagac catatggata cctacgggca
    15061 cagcgtcgaa aaactgcatc aggcgtttat cgatttcatc caccgtatgc ccttctacgg
    15121 caaagccttt ttgtgtgttg acagcgaaca cgtccgcgcg attttgccca aagtgagcaa
    15181 accttatgct acttacggtt tggacgatac cgccgacatc tacgccaccg acatcgaaaa
    15241 cgtcggcgcg caaatgaaat tcaccgtcca tgttcaaatg aaaggacatg agcaggggtc
    15301 gtttgaagtc gtgctgaata tgcccggcag acacaacgtg ctgaacgcat tggcagccat
    15361 cggcgtggcg ctggaagtcg gcgcatcggt tgaagcgatc caaaaaggct tgctcggctt
    15421 tgaaggcgtc ggccgccgct tccaaaaata cggcgacatc aagttgccaa acggcgggac
    15481 cgctttgctg gtggacgatt acggacacca ccccgtcgaa atggcggcaa cccttgccgc
    15541 tgcacgcggc gcgtatccgg aaaaacgttt ggtgctcgcc ttccagccgc accgctatac
    15601 ccgcacgcgc gatttgtttg aagactttac caaaatcctc aataccgttg atgcgctggt
    15661 actgaccgaa gtttatgccg ccggcgaaga gccggttgcc gccgccgact cccgcgcctt
    15721 ggcgcgtgct atccgcgtat tgggcaaact tgagccgatt tactgcgaaa acgtcgccga
    15781 cctgccgcaa atgctgatga atgttttaca ggatggcgat gttgtgttga atatgggtgc
    15841 gggaagcatc aaccgcgttc cctccgcgct gttggaattg tcgaaacaga tttgagatac
    15901 acccgcctga cagacggaac atcatataaa ggtcgtctga aaccgcaatc aggtttcaga
    15961 cgacctctga caacaagcat aaagcaatta ggaaagaaca aaaacaatgc agaattttgg
    16021 caaagtggcc gtattgatgg gtggtttttc cagcgaacga gaaatctcgc tggacagcgg
    16081 taccgccatt ttgaacgcct taaaaagcaa aggcatagac gcatacgcct tcgaccctaa
    16141 ggaaacgccg ttatccgaac tgaaggagcg gggctttcag acggcattca acatccttca
    16201 cggtacttac ggcgaagacg gggctgttca gggtgcattg gaactgttgg gcattcccta
    16261 taccggcagc ggtgtcgccg cctccgccat cggcatggac aaataccgct gcaaactgat
    16321 ttggcaggca ttgggattac ccgttcccga gttcgccgta ctgtacgatg ataccgattt
    16381 cgatgccgtc gaagaaaaat tgggtctgcc gatgtttgtg aagccggcgg ccgaaggcag
    16441 cagcgtcggc gtggtaaaag tcaaagaaaa aggccgtctg aaaagcgttt acgaagaatt
    16501 gaaacacctt cagggcgaaa tcattgccga acgttttatc ggcggcggcg aatattcctg
    16561 ccccgtcctg aacggcaaag ggctgcccgg catacacatc atccccgcaa ccgagtttta
    16621 cgactacgaa gccaagtaca accgagacga caccatttat caatgtcctt cggaagattt
    16681 gaccgaagcc gaagaaagcc tgatgcgcga actggcggtt cgcggcgcac aggcaatcgg
    16741 tgcggaaggc tgcgtgcgcg tcgatttcct caaagatacc gacggcaaac tctatctgtt
    16801 ggaaatcaac accctgcccg gtatgaccgg ccatagttta gtaccgaaat ccgctgccgt
    16861 tacgggcgtg ggttttgccg atttatgtat tgaaattttg aaggccgcac atgtgggata
    16921 atgccgaagc gatggaacgg ctgacgcgct ggctgcttgt catgatggcg atgctgcttg
    16981 ctgcgtccgg gctggtttgg ttttacaatt cgaatcatct gcccgtcaag caggtgtcgc
    17041 tgaagggcaa cctggtttat tccgataaga aggcattggg cagtttggcg aaagaataca
    17101 tccatgggaa tattttgagg acggacatca atggcgcaca ggaagcctac cgccggtatc
    17161 cgtggattgc gtcggtcatg gtgcgccgcc gttttcccga tacggttgag gtcgtcctga
    17221 ccgagcgcaa gccggttgca cgttggggcg accatgcctt ggtggacggc gaaggcaatg
    17281 tttttgaagc ccgcttggac agacccggaa tgccggtatt cagaggcgcg gaaggaacgt
    17341 ctgccgaaat gctccgccgt tatgacgaat tttcgactgt tttggcaaaa cagggtttgg
    17401 gcatcaaaga gatgacctat acggcacgtt cggcgtggaa tgtcgttttg gacaacggca
    17461 tcaccgtcag gctcggacgg gaaaacgaga tgaaacgcct ccggcttttt accgaagcgt
    17521 ggcagcatct gttgcgtaag aataaaaatc ggttatccta tgtggatatg aggtataagg
    17581 acggattttc agtccgccat gctcccgacg gtttacccga aaaagaatcc gaagaatagt
    17641 gggaacaggt atcggacata ttacggccgt gccgtctgaa acggtgcgac gcaaatttca
    17701 atcagtttta agggcagacg aacaatggaa cagcagaaaa gatacatcag cgtactggat
    17761 atcggtacgt ctaaagtcct cgcactgatc ggggaagttc aagatgacga caaaatcaac
    17821 atcatcggtt tggggcaggc tccttcacgg ggcttgcgcg cgggcatggt aaccaatatc
    17881 gatgccaccg tccaagccat caggcaggcg gtcaatgatg ccgagctgat ggcggatacc
    17941 aaaattaccc acgttaccac aggtatcgca ggcaaccaca tccgcagcct taattcgcaa
    18001 ggtgtggtta aaatcaaaga cggagaagta acacaggcag acatcgaccg cgccattgaa
    18061 acggcaaagg caatcaatat cccgcccgat caaaaaattc tcgatgccgt ggttcaagac
    18121 tacattatcg acacccaact tggtgtaagg gagcccatcg gtatgagcgg tgtgcgtctg
    18181 gatacgcggg tgcacatcat taccggtgca agtacggcag tgcagaatgt ccaaaaatgt
    18241 atcgagctgt gcggcttgaa aagcgatcag atcatgcttc aaccgttggc aagcgggcag
    18301 gcggtgctga ctgaagatga aaaagacctc ggcgtatgcg tcatcgacat tggtggcgga
    18361 acgaccgata ttgccgttta tatgaacggc gccatccgcc atacgtccgt cattccggcc
    18421 ggtggtaatc tgattaccaa agatttgtcc aaatcgttga gaacacctct cgatgccgcc
    18481 gagtacatta aaatccatta tggcgtggca tcatgcgata cggaaggctt gggcgagatg
    18541 attgaagttc cgggcgtggg tgaccggaca tcgcgtcagg tttccagtaa ggttttggca
    18601 gcaatcatca gcgcacgtat tcaggagatt tttggcgtag tgctgggcga actgcaaaaa
    18661 tcgggtttcc ccaaagaagt gctgaatgcg ggtatcgttc tgaccggcgg tgtgtccatg
    18721 atgaccggga ttgtggaatt tgccgaaaaa atcttcgatt tgcctgtacg caccggtgcg
    18781 ccccaagaaa tgggcggttt gtccgaccgt gtccgcacac cgcgcttttc taccgctatc
    18841 gggctgcttc atgcagcatg caagctggaa ggaaacttgc cgcaaccgga aaacggtaca
    18901 gtgcaagaga gagaaggggg cggcggtttg ttggcaagat tgaagcggtg gattgaaaac
    18961 aacctctgaa cagttggatt gccgtttgac aggtgagaag tattttgcca gcagtaagat
    19021 acttcttata taataaataa taatttattt aaaccgtcct ctgaacgggg cgatcaggag
    19081 tttttgaatg gaatttgttt acgacgtggc agaatcggca gtcagccctg cggtaattaa
    19141 agtaatcggc ttgggcggcg gcggttgcaa tgcaatcaat aacatggttg ccaacaatgt
    19201 gcgcagtgtg gagtttatca gtgccaatac agatgcgcag tctttggcaa aaaaccatgc
    19261 ggcgaagaga atccagttgg gtacgaatct gacacgcggt ttgggcgcgg gtgcgaatcc
    19321 cgatatcggc cgtgcggcag cacaggaaga tcgggaggcc attgaagaag ccattcgcgg
    19381 tgccaatatg ctgtttatca cgaccggtat gggcggcggt accggtaccg gttccgcgcc
    19441 ggttgttgct gagattgcca agtctttggg cattctgacc gttgccgtag ttacccgtcc
    19501 gttttcttat gaaggcaaac gcgtccatgt ggcacaggca gggttggaac agttgagaga
    19561 acacgtcgat tcgctgatta tcatcccgaa cgacaaactg atgaccgcat tgggtgaaga
    19621 cgtaactatg cgcgaagcct tccgcgcggc ggacaatgta ttgcgcgatg cggtcgcagg
    19681 catttccgaa gtggtaactt gcccgagcga aatcatcaac ctcgactttg ccgacgtgaa
    19741 aaccgtgatg agcaaccgcg gtatcgctat gatgggttcg ggttatgccc aaggtatcga
    19801 ccgtgcgcgt atggcgaccg accaggccat ttccagtccg ctgctggacg atgtaacttt
    19861 ggatggtgca cgcggtgtgc tggtcaatat tacgactgct ccgggttgcc tgaaaatgtc
    19921 cgagttgtct gaagtcatga aaatcgtcaa ccaaagcgcg catcccgatt tggaatgcaa
    19981 attcggtgcc gctgaagacg agaccatgag cgaagatgcc atccggatta ccattatcgc
    20041 taccggtctg aaagaaaaag gagcggtcga tcctactccg gcaagggaag tagaagcggt
    20101 tgccccgtcc aaacaggagc aaagccacaa tgtcgaaggt atgatccgca ccaatcgcgg
    20161 tatccgcacc atgaatctga cagcagcgga tttcgataac cagtctgtcc ttgacgattt
    20221 tgaaattcct gcaattttgc gtcgtcaaca caattcagac aaataatgtg ccgtctgccg
    20281 taaacttatt gcctcccgaa ccggttttcc cgatttggga ggtatatttt tcaatatgcc
    20341 gaatcctgtg cggtttgcgg tgtgcgggtt cagatttttt acttgaaacg gactgctaga
    20401 tatggatatt tcaaaacaaa cattgctgga tagggttttt aacctgaagg caaacggtac
    20461 gacggtacgt accgagttga tggcgggttt gacgaccttt ttgacgatgt gctacatcgt
    20521 tatcgtcaat cccctgattt tgggcgagac cggaatggat atgggggcgg tattcgtcgc
    20581 cacctgtatc gcatccgcca tcggctgttt tgtcatgggt tttatcggca actatccgat
    20641 tgcgcttgcc ccggggatgg ggctgaatgc ctatttcacc tttgccgtcg ttaagggtat
    20701 gggcgtgcct tggcaggtgg cgttgggtgc ggtgttcatt tccggtctga ttttcatcct
    20761 gttcagcttt tttaaagtca gggaaatgct ggtcaacgca ctgcctatgg gtttgaaaat
    20821 gtcgattgcc gccggtatcg gtttgttttt ggcactgatt tccctgaaag gcgcaggcat
    20881 tatcgttgcc aatccggcaa ccttggtcgg cttgggcgat attcatcagc ccagcgcact
    20941 gttggcattg ttcggttttg tcatggtggt cgtattgggg tatttccgcg ttcaaggcgc
    21001 aatcatcatc accattctga cgattaccgt cattgccagc ctgatgggtt tgaacgagtt
    21061 tcacggcgtg gtcggcgaag taccgggcat tgcgccgacc tttatgcaga tggattttaa
    21121 aggtctgttt accgtcagca tggtcagcgt gattttcgtc ttcttcttgg tcgatttgtt
    21181 tgacagtacc ggaacgctgg tcggcgtatc ccaccgtgcc ggactgctgg tggacggtaa
    21241 gctgccccgc ctgaaacgcg cactgcttgc agactctacc gccattgtgg caggtgcggc
    21301 tttgggtact tcttcaacca cgccttatgt ggaaagcgcg gcgggcgtat cggcaggcgg
    21361 acggaccggc ctgacggcgg ttaccgtcgg cgtattgatg ctggcgtgtc tgatgttctc
    21421 cccattggcg aaaagtgttc cggtatttgc caccgcgccc gcactgcttt atgtcggcac
    21481 gcagatgctc cgcagtgcga gggacattga ttgggacgat atgactgaag ccgcgcccgc
    21541 gttcctgacc attgtcttca tgccgtttac ctattcgatt gcagacggca tcgccttcgg
    21601 cttcatcagc tatgccgtgg tcaaactttt gtgtcgccgg actggggacg tgccgcctat
    21661 ggtatgggtt gttgccgtat tgtgggcatt gaaattctgg tatttgggct gattgattcg
    21721 at
//
LOCUS       DAXFZR010000032        21542 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000032 DAXFZR010000000
VERSION     DAXFZR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21542
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.032"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1565
                     /locus_tag="ACFJAS_001018"
     CDS             <1..1565
                     /locus_tag="ACFJAS_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HFC3982295.1"
                     /translation="LWNPAEPAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAA
                     AFKHLLSPWAADPYDAARFAGVFFAVIGLTSCGFAGFNFLGRHHGRSVVLIHIGCIGL
                     IPVAHFLNPAAAAFAAAGLVLHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALM
                     LPLPVLMFFRPWQSRRLMLTAVASLAFALPLMTVYPLLLAKTQPALFAQWLNYHVFGT
                     FGGVRHIQTAFSLFYYLKNLLWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLV
                     LLAFNPQRFQDNLVWLLPPLALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGF
                     FAMNYGWPAKLAERAAYFSPYYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTN
                     WAAGVTLTWALLMTLFLPWLDAAKSHAPVVRSMEASFSPELKRELSDGIECIGIGGGD
                     LHTRIVWTQYGTLPHRVGDVRCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALI
                     RKIGENILKTTD"
     gene            1772..2377
                     /locus_tag="ACFJAS_001019"
     CDS             1772..2377
                     /locus_tag="ACFJAS_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC3982296.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            2449..2841
                     /locus_tag="ACFJAS_001020"
     CDS             2449..2841
                     /locus_tag="ACFJAS_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC3982297.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            2846..2971
                     /locus_tag="ACFJAS_001021"
     CDS             2846..2971
                     /locus_tag="ACFJAS_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982298.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNLFRRIETVLPLPIRY"
     gene            complement(3040..3486)
                     /locus_tag="ACFJAS_001022"
     CDS             complement(3040..3486)
                     /locus_tag="ACFJAS_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC3982299.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(3618..3830)
                     /gene="rpsU"
                     /locus_tag="ACFJAS_001023"
     CDS             complement(3618..3830)
                     /gene="rpsU"
                     /locus_tag="ACFJAS_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC3982300.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(4120..5970)
                     /gene="ltgA"
                     /locus_tag="ACFJAS_001024"
     CDS             complement(4120..5970)
                     /gene="ltgA"
                     /locus_tag="ACFJAS_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC3982301.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            6347..7084
                     /locus_tag="ACFJAS_001025"
     CDS             6347..7084
                     /locus_tag="ACFJAS_001025"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC3982302.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            7086..7772
                     /locus_tag="ACFJAS_001026"
     CDS             7086..7772
                     /locus_tag="ACFJAS_001026"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC3982303.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            7931..8797
                     /locus_tag="ACFJAS_001027"
     CDS             7931..8797
                     /locus_tag="ACFJAS_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC3982304.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(8860..9429)
                     /locus_tag="ACFJAS_001028"
     CDS             complement(8860..9429)
                     /locus_tag="ACFJAS_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC3982305.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            9587..10447
                     /locus_tag="ACFJAS_001029"
     CDS             9587..10447
                     /locus_tag="ACFJAS_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC3982306.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            11121..11474
                     /locus_tag="ACFJAS_001030"
     CDS             11121..11474
                     /locus_tag="ACFJAS_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC3982307.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVMLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            11464..12330
                     /gene="atpB"
                     /locus_tag="ACFJAS_001031"
     CDS             11464..12330
                     /gene="atpB"
                     /locus_tag="ACFJAS_001031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC3982308.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            12387..12623
                     /gene="atpE"
                     /locus_tag="ACFJAS_001032"
     CDS             12387..12623
                     /gene="atpE"
                     /locus_tag="ACFJAS_001032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC3982309.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            12693..13163
                     /locus_tag="ACFJAS_001033"
     CDS             12693..13163
                     /locus_tag="ACFJAS_001033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC3982310.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            13168..13701
                     /locus_tag="ACFJAS_001034"
     CDS             13168..13701
                     /locus_tag="ACFJAS_001034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC3982311.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            13712..15259
                     /gene="atpA"
                     /locus_tag="ACFJAS_001035"
     CDS             13712..15259
                     /gene="atpA"
                     /locus_tag="ACFJAS_001035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC3982312.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            15284..16159
                     /gene="atpG"
                     /locus_tag="ACFJAS_001036"
     CDS             15284..16159
                     /gene="atpG"
                     /locus_tag="ACFJAS_001036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC3982313.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMSLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            16197..17594
                     /gene="atpD"
                     /locus_tag="ACFJAS_001037"
     CDS             16197..17594
                     /gene="atpD"
                     /locus_tag="ACFJAS_001037"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC3982314.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            17605..18027
                     /locus_tag="ACFJAS_001038"
     CDS             17605..18027
                     /locus_tag="ACFJAS_001038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC3982315.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGILEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            18199..19095
                     /gene="glyQ"
                     /locus_tag="ACFJAS_001039"
     CDS             18199..19095
                     /gene="glyQ"
                     /locus_tag="ACFJAS_001039"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC3982316.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            19212..21275
                     /gene="glyS"
                     /locus_tag="ACFJAS_001040"
     CDS             19212..21275
                     /gene="glyS"
                     /locus_tag="ACFJAS_001040"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC3982317.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGRQDVYAYEYVQTGKPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEIIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTENPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAGRAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAALGEVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFRTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            21339..>21542
                     /locus_tag="ACFJAS_001041"
     CDS             21339..>21542
                     /locus_tag="ACFJAS_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3982318.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQN"
BASE COUNT         4992 a         5784 c         5701 g         5065 t
ORIGIN      
        1 atttgtggaa tcctgccgaa cctgccgtct ataccgccgt cgaagcactg gcaggcagcc
       61 ccaccccctt ggttgcccat ctgttcggtc aaaccgattt cggcataccg cccgtgtatc
      121 tttgggttgc cgccgcattc aaacatttgc tgtcgccgtg ggcagccgac ccgtatgatg
      181 ccgcacgctt tgcaggcgta ttttttgccg ttatcggact gacttcttgc ggctttgccg
      241 gtttcaactt tttgggcaga caccacgggc gcagcgttgt tttaatccat atcggctgta
      301 tcgggctgat tccggttgcc catttcctca atcccgccgc cgccgccttt gccgccgccg
      361 gactggtgct gcacggctac tcgctggcac gccggcgcgt gattgccgcc tctttcctgc
      421 tcggtacggg ttggacgttg atgtcgctgg cggcagctta tccggcggcg tttgcgctga
      481 tgctgcccct gcccgtgctg atgtttttcc gtccgtggca aagcaggcgt ttgatgttga
      541 cggcagtcgc ctcgcttgcc tttgccctgc cgcttatgac cgtttacccg ctgctcttgg
      601 caaaaacgca gcccgcgctg tttgcgcaat ggctcaacta tcacgttttc ggtacgttcg
      661 gcggcgtgcg gcacattcag acggcattca gtttgtttta ctatctgaaa aatctgcttt
      721 ggttcgcact gcccgcgctg ccgctggcgg tttggacggt ttgccgcaca cgcctgtttt
      781 cgaccgactg ggggattttg ggcattgtct ggatgcttgc cgttttggtg ctgctcgcct
      841 ttaatccgca gcgttttcaa gacaacctcg tctggctgct gccgccgctt gccctgttcg
      901 gcgcggcgca actggacagc ctgaggcgcg gcgcggcggc ttttgtcaac tggttcggca
      961 ttatggcgtt cgggctgttt gccgtgttcc tgtggacggg ctttttcgcc atgaattacg
     1021 gctggcccgc caagcttgcc gaacgcgccg cctacttcag cccgtattac gttcccgaca
     1081 tcgatcccat tccgatggcg gttgccgtac tgttcacacc cttgtggctg tgggcgatta
     1141 cccggaaaaa catacgcggc aggcaggcgg ttaccaactg ggcggcaggc gttaccctga
     1201 cctgggcttt gctgatgacg ctgttcctgc cgtggctgga cgcggcgaaa agccacgcgc
     1261 ccgtcgtccg gagtatggag gcatcgtttt ccccggaatt aaaacgggag ctttcagacg
     1321 gcatcgagtg tatcggcata ggcggcggcg acctgcacac gcggattgtt tggacgcagt
     1381 acggcacatt gccgcaccgc gtcggcgatg tccgttgccg ctaccgtatc gtccgcctgc
     1441 cccaaaacgc ggatgcgccg caaggctggc agacggtctg gcagggtgcg cgcccgcgca
     1501 acaaagacag taagtttgca ctgatacgga aaatcgggga aaatatatta aaaacaacag
     1561 attgagccga atttctggat taagtgccgg aaatcgcgta taattgcgcg attaaacctt
     1621 tatatagtgg attaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     1681 tacggcaagg cgaggcaacg ccgtaccggt ttaaatttaa tccactataa atcagccgtt
     1741 ttgcaggcat cacacaggag cgacaattat tatgatgacc ctctattccg gcattacctg
     1801 ccccttcagc caccgctgcc gcttcgtttt gtacgaaaaa ggtatggatt ttgaaatcaa
     1861 agatatcgat atttacaaca aacccgaaga cctcgccgtc atgaacccgt acaaccaagt
     1921 ccccgtgctg gtcgagcgcg atttggtgct gcacgagtcc aacatcatca acgaatacat
     1981 cgacgaacgc ttcccccatc cgcagcttat gcccggcgat cccgttatgc gcggccgggg
     2041 gcggctggtg ctgtaccgta tggaaaaaga attgttcaac cacgtccaag ttttggaaaa
     2101 ccccgccgcc gccaacaagg aacaggcaaa agcgcgcgaa gccatcggca acggcctgac
     2161 gatgctttcc ccttcgttca gcaaaagcaa atacatcctc ggcgaagatt tttctatgat
     2221 tgatgttgcc ctggccccgc tgttgtggcg gctcgaccat tacgatgtca aactgggcaa
     2281 aagtgccgcg ccgctgctca aatacgccga gcgtatcttc cagcgcgaag cctttatcga
     2341 agcactgaca cccgccgaaa aagccatgcg caaataagtc cgaaatgctt gcaaaacccg
     2401 ccgttttgca ggcattttca cattcaggca caccacacgg aacccatcat gcccacctca
     2461 accaaaccct acatcctccg cgcactttgc gaatggtgca gcgacaacag cctcacgccg
     2521 cacatccttg tctgggtaaa cgagcatacg cgcgtcccca tgcagtacgt ccgcgacaac
     2581 gaaattatgc tcaacatcgg cgcgaccgcc acgcaaaacc ttcgaatcga caacgattgg
     2641 atcagctttt ccgcccgttt cggcggacaa gcgcacgaca tatggatacc tgtcggacac
     2701 gtcctcagcc ttttcgcacg ggagaccgga gaaggtatgg ggtttgaatt ggaggagtac
     2761 cgccccgaca cgccgtctga aaacacttct gccgaaaccg cgccccggcc cgccaaaaaa
     2821 ggtttgaaat tggtcaaata aatctatgcc gtctgaacgg aatcgtgttt cagacggcat
     2881 tttgtccgat ggggcgcaaa cggaatccgt ttatcggcaa aacctgtttc ggcgtatcga
     2941 aaccgtgttg cccctgccga ttcgatatta ggacattgaa aatgccgtct gaacctgcga
     3001 tacgggcttc agacggcgtt ttgtccgata ttcgggcaat caggcggtca gtacggtttt
     3061 caagattttg ttgacttcgc ccatatcggc tttgccggcg aggcgggttt tcaatacgac
     3121 catcactttg cccatatccg ccatacctgc cgcgccggtt tcggcaacgg ctgcttcgac
     3181 ggcggtgcgg atttcgccgg cggagagcat ttgcggcagg tagcggtgca gcacgtcgat
     3241 ttcggcgttt tctttgtctg ccaaatcctg acggccggct tcagtgtaga ttttcgcgct
     3301 gtctttgcgc tgtttgacca ttttggtcag gatggcggtg attttggcat cgtcggcttc
     3361 ggtgcgttcg tctacttcaa actgtttgac ggcggcattg atgaggcgga tggtgccgag
     3421 ggaaacttga tctttggcgc gcatcgcggt tttcatatct tcggtaaggc gggttttcag
     3481 gctcatgatg tcctcgctgg gatgtcggat ggaaacggcg ggtttcgatg ccgtctgaaa
     3541 agcaaaacac accgcagagc aggtcttgcg gtgtgtcttc atatcacagg gctgacctgt
     3601 aatctgtact tgaacgttta gtacattttg ggcggcagct gttggctgcg caggcgtttt
     3661 tgcaggcgtt ttacggctgc cgcttttttg cgtttgcgtt cggtagtcgg tttttcgtag
     3721 gcttcgcggg cgcgcagctc ggtcagcagg ccggtttttt ctacggcgcg tttgaaacgg
     3781 cgcatagcga cttcaaaggg ttcgttctct tttacgcgga ttgcaggcat tttatttcct
     3841 ttaataaatt cggttgttcc atctgcccat cataatcggt ggaaagggta gggcagacgg
     3901 tgttgtaggt tttccgtatc gctgtgcctt tcggcggcgg gatacgcgcc gtgacggaaa
     3961 catcacctcc taacgggtcg gcttgtgccg tgtcaagctg cttgaaaaag taggaaaaac
     4021 aattttcgga ttgtcttata tttatggttc gccgtcaatg ccgttcggga taaaaatgcc
     4081 gtctgaaaga ccgggcgggt ttcagacggc atcggcacgt cagcgtgcgg gaacggtgcc
     4141 catacgctgt ttgagcggga tgtgcggcgc gccgaagagg gaggcgtagt aggcggcatt
     4201 ggtcatcact tttttgacat agtcgcgcgt ttcggaaaac gggatggttt cggcatatac
     4261 cgcgccttcg aggggtgtgt ccgcctgcca tcggcgcgcc ctgccggggc cggcgttata
     4321 gcctgcggtg gcgaggattt cgttgttttg caggcggcgt ttggtgtccg ccatatacca
     4381 cgtccccata cggatattgc cgtcggcggt gtagagctgg gcggcatcca taccgatttt
     4441 gccggcgatt tcgcgcgcgg tggcaggcat aacctgcatc agcccctgcg cgcctacgcg
     4501 ggattgcgcg cctataacga agcggctttc ctgacgaatc aggccgtaaa cccaagccgg
     4561 atcgacatta acattttgcg cgtggcggat tacggtgtct ttaaacggcg aaatgtagcg
     4621 caatgtgtag ttgagtttgc ggtcggtgcg ttccgcgctg ttgaccgcca tatcgtaaaa
     4681 accgtggtcg aacgcggttt gcgcggcggt cagcagcttg tcttcgtcaa agccgcgtgt
     4741 ggcaaaacgc cattccgcct gagcctgacg gcgcattttc gcatcgccgg cggttcggct
     4801 gtttcggaac agcaccagcg cgcgcttaat cgcgccgtct tccgccatac ggaggacact
     4861 gtttttgccg gcatcgggca cattgttgcg cgtatcgatt ttgcgcccca attcttcccc
     4921 tgccagtacg gcataaaaat tcctgcccgt cgctgccgcc tgtttgtaaa gtttttccgc
     4981 ttcttgcgtg ttgcccgttg cggcgcggct gcgtgccagc cagtagagcc aggtcgggct
     5041 tttttgcagt ttttcgggca tatgcgagat cacggaggcc agctcgtccc aacgtcgggc
     5101 gcgcaaggcg gcgcgggcgt accactcgat ttggtcgtcg gtcagttggc ggcggtcggc
     5161 aaccttgccg taatagtcca aggcggcagg cacattgagg ctttgcgact gataatgccc
     5221 caatacgccc cacgcgaaac tgcgttgttc ggggcttaaa ccgctttcca tttcggacag
     5281 cagggcggcg gcattcggcg atttgcgtgc ttctttgccg atgacgttca acagggcata
     5341 ttcgcgcgaa ccttgtgtac cgccgtcaaa cgggctgccc aatgcggcgg caaggttgcg
     5401 cgcgtccgtc gtttggcggc cggccagcag tccgcgcacg cgcctccagg catcgttgcc
     5461 gtccaacaag ccggatgcgg ctgcctgttc caacagtttg gtgcagcccg aaggcagttt
     5521 gcccgtattt ttgaccagtt cggcggcacg cgtatagtcg ttgcggctcg aatcggcgta
     5581 gcattcgact tcttgggcgc gcccttcagg tttgagtttg gcatattcct gtgcaaacag
     5641 cgtccactgt ctgcgcgcgc ccaaagactt cagccactcg ttgcggacat tttccgccat
     5701 cgcgctgtcg ccggcgtttt ccaaataggc ggcggctgcc gcatcgttgt tctgtttcac
     5761 tgcatccagt gcggacgggt agccgccgta atctgccagc gtttttcctt ccggttcggc
     5821 agggcgggtg ggaacgcttg ccgaaaggtc ggcagtttct atattgtctg ccggggtctt
     5881 gccggctggc agtgtgtttg tcgaagaaca cgcggcaagc accagggccg ccagcagcgg
     5941 cagggaatgc ttcatagagg gtaggtacat cggatttcct taagaatcgg aaccctgaac
     6001 ggtcagggtt ggaaaagaca aaatgccgtc tgaacaggcg tttgcccgaa ttatatgccg
     6061 aaactgcacc gtctttggaa tgtttccgac ataatttata tttttcaatc atttgccgtt
     6121 tgggtgcgaa ccgctgcctt tgcccgtttc agacggcatt gtccgaaatg gttgcccgct
     6181 tcctgcctta ttgacaaaaa aacgctttcc cgataatatc ctacgaaaat taacctgccg
     6241 atttgacaca gcttgcgggc ataacagcta aagcgttccg acaatttcag ctttatcttc
     6301 cgcgcccgtt gtgtccgaca tcgggctttg ttgtatggga aagacaatga ttattttgga
     6361 caaggtttcc aagcattacc aaacgcgcga caaaacccgt tttgccgccg tcgagccgac
     6421 cagcctcgaa attcaagacg gcgaaatctt cgggctgatg ggctattccg gcgcgggcaa
     6481 atccactttg ttgcgcctga tcaacctgtt ggaacgcccc gacacgggca aagtcaacgt
     6541 ctgcggacaa gaactgaccg cgctcgatgc cgccgcattg cgtcaggcgc ggcagaatat
     6601 cggcatggtg tttcagcagt tcaacctttt aagcaaccgc accgtcgccg gcaacgttgc
     6661 ctttccactg gaaatcgccg gatggccgtc tgaaaaaatc aaagcgcgcg ttgccgagtg
     6721 cctcgaaatc gtcggcttga ccgaacgcgc cggccactac cccgcccagc tttcaggcgg
     6781 gcagaaacaa cgcgtcggaa tcgcccgcgc gctcgcgccc aagccgcaag tcatcctcgc
     6841 cgacgaaccc acttccgccc tcgaccccgc caccacgcgc agtgtcttgg aatgtttgga
     6901 agacatcaac aaacgcttca acgttaccat cgtcatcgta acccacgaaa tgagcgtcat
     6961 cagacgattg tgcgaccgcg ccgccctgtt ggataaaggg aaagtggtgg aaatcgtcga
     7021 agtacgcggc aaccaaatcc acgctcaatc cgaaatcggg cgcgaactga ttcgggagga
     7081 ctgatatggc agacttaaca ttcgaacaag ccgtttccac catcgtcggc atgaaagacg
     7141 aaatcgtccg cgccttgggc gaaactttcg tgatggtcgg cttgtccacc acattcgccg
     7201 tcatcttcgg cacgctcttg ggcgtgttgc tcttcgtaac ttccagccgc caactgcatt
     7261 acaacaagcc ggtaaacttc ctgctcgaca acctcgtcaa cctcatgcgc gccttcccct
     7321 tcgtcatcct gatgattgcg atgatacccg ccacacgcgc catcgtcggc agcaccatcg
     7381 gcccggttgc cgcctcgctg gtgttgagcg tgtcgggatt gttttatttt gcccgactgg
     7441 tggaacaaaa cctgcgcgaa gtcccaaaag gtgtgattga agccgccgcc gcgatgggtg
     7501 cgccgccgat tgccatcgtc ctcaaagtcc tcttgaacga agcgcgcgcg ggcatggttt
     7561 ccagcattac cgtgcttgcc atcgggcttt tgtcatacag cgcggcggca ggcatgatag
     7621 gcggcggcgg cttgggcgac ctcgccatcc gctacggcta ctaccgctac caaaccgaag
     7681 tcatcgtctt catcgtcgcc ctcctcgtgc tgctggtcat cctgattcaa agcaccggca
     7741 acgcgttggc gcggaaactc gacaaacgtt aaacccgaat gccgtctgaa cgccaaaaac
     7801 cccgccgcta tccgaaaaat gctataaaat ccccctgttc gcggcaaatg ccgtctgaac
     7861 gccgaatccg gacggcagga ctccctgcct gtcatttttg tttgaaaccg ccacaacatc
     7921 aggagaaaat atgaaaacct tcttcaaaac cctttccgcc gccgcactcg cgctcatcct
     7981 cgcagcctgc ggcggtcaaa aagacagcgc gcccgcagcc tctgccgccg ccccttctgc
     8041 cgataacggc gcggcgaaaa aagaaatcgt cttcggcacg accgtcggcg acttcggcga
     8101 tatggtcaaa gaacaaatcc aagccgagct ggagaaaaaa ggctacaccg tcaaattggt
     8161 cgaatttacc gactatgtgc gcccgaatct ggcattggcg gagggcgagt tggacatcaa
     8221 cgtcttccaa cacaaaccct atcttgacga tttcaaaaaa gaacacaacc tggacatcac
     8281 cgaagccttc caagtgccga ccgcgccttt gggactgtat ccgggcaaac tgaaatcgct
     8341 ggaagaagtc aaggacggca gcaccgtatc cgcgcccaac gacccgtcca acttcgcacg
     8401 cgccttggtg atgctgaacg aactgggttg gatcaaactc aaagacggca tcaatccgct
     8461 gaccgcatcc aaagccgata tcgcggaaaa cctgaaaaac atcaaaatcg tcgagcttga
     8521 agccgcacaa ctgccgcgca gccgcgccga cgtggatttt gccgtcgtca acggcaacta
     8581 cgccataagc agcggcatga agctgaccga agccctgttc caagagccga gctttgccta
     8641 tgtcaactgg tctgccgtca aaaccgccga caaagacagc caatggctta aagacgtaac
     8701 cgaggcctat aactccgacg cgttcaaagc ctacgcgcac aaacgcttcg agggctacaa
     8761 ataccctgcc gcatggaatg aaggcgcagc caaataaggc agtcgtataa aatgatgccg
     8821 tctgaaccgc attccgtgtt cagacggcat ttttgtcctt taatccgcca ttccctgcca
     8881 ttccgccaaa tccggcgtat cgattccgaa cagcgacagc gtgtgtgcga tactgtgcgc
     8941 cactatgtcg tccgccgtct gcggcttgcg gtacatcgca ggaacagggg gaaacaccac
     9001 gccgcccatt tccgttaccc gcttcatatt gtccaaatgg gcaaggttca gcggcgtttc
     9061 gcgcaccatc agcaccagcc gccgcctttc cttcaaaacc acatccgccg cacgcgtcaa
     9121 gagattgtcg ccgaagccgt gcgcgacaga ggcaagcgtc cgcatcgaac agggtgcgac
     9181 cagcatcccg tccgttttaa acgtaccgct ggcaatgcac gccccgatat tgccgatcgg
     9241 atggacgaaa tcagccaagg catatacttc gtctttcgta taatccgttt ccgaagcgcg
     9301 cgccatctcc gcgcctttcg ataccacaag gtgcgtttcg acatcttgcg cgcgcaaaag
     9361 ttccaaagcc ttcacgccgt attggaaacc gctcgccccg ctgatgccga ttatcaaacg
     9421 ccgtaccatc atccgccttt cccataaaac cgcctgcaac ggcaaaccgg ctattatagt
     9481 gaaaaaacag aaatccgata aacacggata caaattgtcg gcaacaccca atatccgata
     9541 aaatacccga tttaacatcc tatctgaata ggcacgggag ggcggtatgg caaaagtaaa
     9601 aggcggattg gggcgcggct tggattcgct gctcgccaac ggtgcggaca acagcagcgg
     9661 cgaccggttg actgcggttg cggttaaaga tatccggccc ggccgctatc aggcgcgcgt
     9721 ccaaatcgat gacgaagcct tgcaggaact ggcagattcg attaaggcgc aaggcgtgat
     9781 acagcccgtc atcgtgcgcg aacacggact gtcccgatac gaactgattg caggcgaacg
     9841 ccgttggcgc gccgctcaga ttgccggcct gtccgaaatc cccgccgtta tcaaaaccat
     9901 cagcgacgaa accgcattgg caatgggttt gatcgaaaac ctccagcgcg aaaacctcaa
     9961 ccccatcgaa gaagcacaag gcttgaaacg ccttgccgac gagttcgggc tgacccacga
    10021 aaccatcgcc caagccgtcg gcaaaagccg aagcgcgatt tccaacagcc tgcgcctttt
    10081 aagcctgccc gaatccgtgc aggaaatgct ttaccaacgc cgcctcgaaa tggggcacgc
    10141 ccgcgcactg ctgaccctgc ccgtcgtcga acagctcgaa ttggcgcaaa aggccgtcaa
    10201 aaacggctgg tcggtgcgcg aagtcgaacg ccgcagccag gccgcccttc aaaacaaacg
    10261 tcccgagcca aaaaaaactg ccgccgccga catcggccgc ctgaatgatt tgctgactga
    10321 aaaattgggt gtcaacgccg aaatcaagac cgccaaccat aaaaaaggca aaatcatcct
    10381 gcatttcgac acgcccgaaa ccttcgacca tatcttgaag cagttgggca tagattaccg
    10441 gccttaattt tgcgggatat gccgtttgaa atatagagaa tagctttcca gattttaagt
    10501 gggaaatata attctattga cattttttct gcttcgcgta agaatcgttt tcctgttttc
    10561 atttttaatt ttcgaggaaa ttatgaacac acgcatcaac tttacgcaac tgtgggtaag
    10621 cgcgtgagcc aatgctatgt taccagtgta atcagcacaa tcggcgttac cacttccgat
    10681 gcaatttgtt cgggaggcgg aacgaacaaa gccaaaagtc aatgcaatgc tttgcttaaa
    10741 gcggcaggcc gctgctaatc ctttattcgg aaaaggtcgt ctgaaaatat tttcagacga
    10801 ccttttattt attgagcaaa ctccccaaac tgccgacaat ccgaccgata aactactaca
    10861 atttcgcgtt gattttttat tatttccacg taaagcaaac aaatcacgta agaacattaa
    10921 aagtgtaaag aaattgcgga caaactcctt aatatcctgt aatcatatgt aatatgtgtt
    10981 agaatagcgg agttggattt tggggagtcg ctcgtctgta tcggcggcga gtttcacaaa
    11041 gattaacata ttgcttgacg taataaggct tctaaattat attagccgcg ccccaaacct
    11101 gaggcggaaa cattcccttt atgaagcaga ttatcatcct acaatccgcc gtattgtcca
    11161 tatgcgccgc agttgccttt gccgtatggg gttttgccgg attcctttcg gcggtcgggg
    11221 gcggtttgtc ctacctgctt cccacgtttg ttgcagtttt acttctaaaa cttttcaggg
    11281 gaaacccctt cctgcaaagc aggatgttcg tcttcggaga gattttaaaa gtaatgctgt
    11341 cgctgttgtc catgcttgcc gtatttgcaa tatggcatca atcgctggtg ttcgtcccgt
    11401 ttttgatggg gctgctcggt gtcagccatc tggttttttt agtattgttg agagtgaaag
    11461 attatggcag gtgaaaccat taccgctgcc gactacatca agcaccactt gcagagcttg
    11521 accagtttgt cggatgttac tcagggtcag ggactgaaaa acattgctga tttttcgttt
    11581 attaaccttg atgccgtttt ttttgccgtc ctgttgggcg taatcggcag cttcctgttg
    11641 tggcgcggtg caaaaaaagc aacggcaggc gttcccggac gtttccaggc cgccgtggaa
    11701 atcttgttcg agtttgtgga cgatatgtgt aagagcatca ttcacagcga aaagtctaga
    11761 aaagccgtcg cgccgttggg tctgacgctg tttgtttgga ttttcctgat gaacgcgatg
    11821 gatatgctgc cggtcgattt gctgccgatg gtatggcagg gcattaccgg caaccatcac
    11881 gccctgctgc gcatcgtacc gactgccgat ttgaacaccg ctttggcact cgctgtcggc
    11941 gtgttgctga tttgtatcta ttacaacatc aaaatcaaag gattgggcgg ctggtttcac
    12001 gagttgttca gtgcgccgtt cggtgcaaaa ctcgcacctg cgaactttct gttgaacttg
    12061 gtcgagtttc tttctaaaac cgtatcccac ggtatgcggt tgttcggcaa tatgtatgcg
    12121 ggcgagctgg tattcctgct gatagccttg cttggcggtg cttgggcggc ttccggcagc
    12181 gttgaagtca tggatccgat tctgtttgta ttccacatta ttgccggttt ggcatgggcg
    12241 attttccata ttttggtgat taccctgcag gcatttattt tcatggcgtt ggcgttcgtc
    12301 tatatcgggc aggcgcacga tgcccactga ttttcccaag tagtttgttt ttgtagttta
    12361 acctttgttt ctttaaggag tttaaaatgg gtttgattgc tatcgcatgt ggtttgatcg
    12421 ttgcattggg tgcattgggt gcatctatcg gtatcgcaat ggtcggttct aaatatttgg
    12481 agtcttctgc tcgccaacct gaactgattg gtccgctgca aaccaaactg ttcctgattg
    12541 ccggtctgat tgatgccgca ttcttgatcg gtgtcgccat tgcactactg ttcgccttcg
    12601 tcaacccgtt tgcaggtgca taatcaggac ggattcgtcc gttcgtacag gtgcagcctg
    12661 tttgattaac cccaatacga aggttaagta acgtgaatat caatgcaaca ttattcgctc
    12721 aaatcatcgt ctttttcggt ttggtatggt ttaccatgaa atttgtgtgg ccgccgattg
    12781 caaaagcttt ggatgagcgt gccgcaaaaa tcgccgaggg cttggctgcc gccgagcgtg
    12841 gtaaaagcga tttcgagcag gctgaaaaaa aggttgcaga acttttggca gaagggcgta
    12901 atcaggtttc cgaaatggtt gccaacgccg aaaaacgtgc cgccaaaatt gtcgaagaag
    12961 ccaaagaaca ggcttcttcc gaggcggcgc gcattgcagc tcaggcaaag gccgatgtgg
    13021 agcaggaatt gttccgcgca cgcgaatccc tgcgcgatca ggttgccgtg ttggctgtca
    13081 aaggtgccga atctattttg cgcagcgaag tcgatgcttc caaacacgca aaactgctcg
    13141 ataccctgaa acaggagttg taatcttatg gcagagttcg caacgatcgc cagaccttat
    13201 gcaaaggcat tgttcggtct ggctcaggaa aaaaaccaaa ttgagtcttg gttgggcgga
    13261 ctggaaaaac ttgcggcggt tgttcaggaa gggaaggtgg cttcattgat tgaccgtcct
    13321 gaaacgaatg cttcagaaaa agcagatatc ctcatcgatt tggtcggttt gaaagacaag
    13381 gagttaaaaa actttgttat cgtcttggcc gggcagaaac gtttgtcgat attgccggaa
    13441 gtgtatgctc aatatcaaga cttgacctta tcattcaacc atatcaaatc tgccgtcatt
    13501 tacagtgcct atccgttgac cgacaaacag gtcggcgagt tggcgcaaat gctgaataag
    13561 cgtttcgaca gcgagctgaa aatctctgtc gaaatcgaac cggagctgat tggcggcata
    13621 aaagttgaag tgggtgatca ggttttggat ttgtctgtac aaggcaaact gagtgctttg
    13681 tacacgacta tgacgaatta ggagagtttt catgcagctt aatcctgctg aaattagcga
    13741 tctgattaaa gccaagatcg aaaatctgtc tgtaaatgcc gaagtgagta cccgtggtac
    13801 ggtgatttcg gtaactgacg gtatcgttcg catccatggt ttgtcagatg caatgcaagg
    13861 tgagatgctc gaattcccgg gtaacacttt aggcttggcg atgaacctgg agcgcgactc
    13921 cgtcggtgcc gtagtgttgg gcgagtacga acatattaaa gaaggcgaca cggttacctg
    13981 taccggccgt attttagaag tgccggtcgg acgcgaattg gtcggacgcg tcgtcgatgc
    14041 attgggtcgg cctatcgacg gcaaaggccc gattaataca actttgaccg cccctgtcga
    14101 aaaaatcgca ccgggcgtga ttgcacgcaa atcggttgat cagccgatgc aaaccggtct
    14161 gaaggcgatt gactctatga ttcctgtcgg tcgcggtcag cgtgagttga ttattggaga
    14221 ccgtcagaca ggtaaaacgg ccgtagcatt ggatgccatc gtcaaccaaa aaggtacggg
    14281 tgttatctgt atctatgtcg ctatcggtca aaaagcatct tctattgcca acgtggtccg
    14341 caaattggaa gagcatggcg cgatggaaca cacgattgtg gttgctgcaa ctgcatctga
    14401 agcggcggca ttgcaatata tcgcacctta ttcaggttgt acgatgggtg aattcttccg
    14461 cgatcgtggc gaagatgcct tgattgttta tgacgatttg tccaaacagg ctgtggctta
    14521 ccgtcaaatt tccctgcttt tgcgccgtcc gcccggccgc gaagcctatc ccggcgatgt
    14581 gttctacctg cactcccgtc tgttggaacg tgcggcacgt gtcaatgaac acgaagtgga
    14641 aaaattgacc aacggcgaag taaaaggcaa aaccggttct ctgaccgcgt tgccgattat
    14701 cgaaacccaa gcgggcgacg tatctgcttt cgttccgact aacgtcattt cgattaccga
    14761 cggtcagatt ttcttggaaa ccgacctctt caacgccggt atccgtcctg caatcaatgc
    14821 cggtatttcc gtatcccgcg taggcggtgc tgcacaaacc aaagtgatta aaaagctggg
    14881 tggcggtatc cgtttggcgt tggcacaata tcgtgaattg gcggcgttct cgcaatttgc
    14941 atccgatttg gatgaagcta cgcgcaaaca gttggagcat ggcgaagtcg taaccgaact
    15001 gatgaaacag aaacagttca gtacgttgaa tacggctgaa atggctttga ccctttgggc
    15061 aatcaacaac ggttcgtatt ctgatgttcc ggttgccaaa gctctagctt ttgaatctga
    15121 atttttgagc tttgtccgta cccaacatcc ggaagttttg gaagccgtca atgcttcagg
    15181 tgcaatgtcc gacgagagcg agaaaacgct tgaagcagcc atgaaatcct tcaaatcttc
    15241 ttacgcctat caggcataag gcttgaagtg aaaggagtct gaaatggcag taggaaaaga
    15301 gattctcacc aaaatccgca gtgttcagaa tacccaaaag atcactaaag cgatgcaaat
    15361 ggtgtcaacc tctaaaatgc ggaagactca ggaacggatg agtttggcgc gtccgtatgc
    15421 cgaaaaagtg cgtatggtga tgagccatct tgcgcaaacc aataccgatc atggtattcc
    15481 gttactggaa tctcatcggg aaatcagacg tgtcggtttt attttgatta cgtctgataa
    15541 gggtttgtgt ggcggtttga acgccaacgt gctgaaaaag tttttggcac aagttcaaga
    15601 gtatcggaat caaggtattg aagaggaaat cgtatgcctt ggcagtaaag gtctgatggc
    15661 gtgtcagagc attggtctga atgtggttgc cagtgccgta aatttgggcg ataccccaaa
    15721 aatggaaatg ctgctcggac ctttgacaga acttttccaa cggtatgaga aacatgaaat
    15781 tgacagaatc catctggtgt attcgggttt tgtcaatacc atgcgtcaag aaccgaggat
    15841 ggaagtattg ctgcctatcg gtgagaacgt gattggcgat tcagctccca aatcaccgtt
    15901 cagctgggaa taccgctacg aaccgactgc acttgcagtg ttggaatatc tggttcgccg
    15961 ctatttagag tctgtggttt atcaggcgtt gagcgacaat atggcatccg aacaggcagc
    16021 ccgcatggtt gccatgaaag ctgcaacaga caatgcaggc aatgccatca aagagttgcg
    16081 tctggtatat aacaaatcgc gtcaagctgc gattaccacg gaattgtcag aaattgtagc
    16141 aggtgcggcg gccgtctgat gccgtctgaa tcctgtacgg aaattaggat acgataatga
    16201 gccaaggcaa aatcgtacaa attatcggtg cggttgttga cgtggaattt ccacgcgaca
    16261 tgattccgcg cgtttacgac gctttgaaat tagacgaaaa cggtctgact ttggaagtcc
    16321 aacagctttt gggtgatggc gtagtccgta ccatcgctat gggcagctcg gacggtttga
    16381 aacgcggcat gactgtgagc aatactggtt cgcccattac tgtgccggta ggtaaaggta
    16441 cgttgggacg cattgtcgat gtattgggaa ctcctgttga cgaggcaggt ccaattgata
    16501 ccgacaagag tcgtgccatc caccaagccg ctcctaagtt tgacgaactg tcttccacaa
    16561 ccgaattgct cgaaacgggc attaaagtga ttgacttgct gtgtccgttt gccaaaggcg
    16621 gtaaagtagg tctgttcggc ggtgccggtg tgggtaaaac cgtgaacatg atggaattga
    16681 tcaacaacat cgccaaagcg cacagcggct tgtccgtgtt ctcaggcgtg ggtgagcgta
    16741 cccgcgaagg taacgacttc taccacgaga tgaaagattc caacgtattg gataaagtag
    16801 ccatggtgta tggccaaatg aacgaacctc caggcaaccg tctgcgcgtt gctttgaccg
    16861 gtttgactat ggctgaatac ttccgtgacg aaaaagacga aaacggtaaa ggtcgtgacg
    16921 tattgttctt cgttgacaac atctaccgtt acactctggc cggtaccgaa gtatccgcac
    16981 tgttgggccg tatgccttct gcagtgggtt accaaccgac attggctgaa gaaatgggtc
    17041 gtttgcaaga gcgtattacc tctacccaaa ccggttccat tacttccatc caagccgtat
    17101 atgtacctgc ggatgacttg actgacccgt ctccggcgac aactttcgcc cacttggacg
    17161 cgaccgtcgt attgagccgt gatattgcct ctttgggtat ttacccggca gttgacccgc
    17221 ttgactctac ttcgcgccaa ttggatccga tggtattggg tcaagagcac tacgacgtag
    17281 cgcgcggtgt acagtctact ctgcaaaaat acaaagaatt gcgcgacatc atcgccattc
    17341 tgggtatgga cgaattgtct gacgaagaca aactggctgt aatgcgtgcc cgtaaaatcc
    17401 aacgcttcct gtctcaaccg ttccacgttg ccgaagtgtt tacaggttct ccaggcaaat
    17461 atgtcgccct gcgcgatacc attgccggct tcaaagccat cttgaacggc gaatacgatc
    17521 atctgcccga acaggcattc tatatggtcg gcagcatcga agaagcggtt gagaaagcga
    17581 aaaccttaaa ctgaggaggt cggcatgagc atcatgcaag ttgaggtggt aagtggcgag
    17641 cagaaaatct attcaggcga ggcaacattt atcgttgttc cgactgtaca gggcgaactc
    17701 ggtatttatc cgcgacacga gccgattatg agtttggtgc gtcccggtgc gttgcgtttg
    17761 accgttccgg gcgaggataa agaggttttg gttgctgttt ccggcggtat tttggaagta
    17821 cagcctgata aagtaactgt cttggcggat gttgccgtcc gcagtgcgga aatggatcgg
    17881 gcacgtgcgg aagaggcgaa aaaagccgca gaagcgggca tttcccaagc taaagacgat
    17941 aaggctttgg cggaagcaca taaagcattg gctgccgcaa ttgcacagct caaaactttg
    18001 gactatatcc gttcgcacaa gaaataaact tgtttggttt gaaaaagcac gaccgtttgg
    18061 ccgtgctttt tattttccgg atttaaatta aaaaattatc catcccctgg tttcaaatca
    18121 ggctgtcttc gagataaatc tattcaagcg gctatggaaa cagtaaaata aaacctttat
    18181 tttatcgaac aataagccat gcttaccttc caacaaatca tcttcaaact gcaaaccttc
    18241 tgggcagaca aaggctgcac cgtcatccaa cccttcgaca tggaagtcgg tgccggcaca
    18301 tcccatcccg ccacctgcct gcgcgcgctc ggccccgagc cttggtttgc cgcctacgtc
    18361 caacccagcc gccgccccaa agacggccgc tacggcgaca accccaaccg cctgcaacac
    18421 tattaccaat tccaagtcgc cctcaaaccc gcccccgcca atatccaaga cctctatctc
    18481 gactccctgc gcgaattggg catcgatccc aaagtccacg acatccgctt tgtcgaagac
    18541 gactgggaaa accccaccct cggtgcgtgg ggtttgggct gggaagtctg gctcaacggc
    18601 atggaagtaa cccagtttac ctatttccaa caagtcggcg gtatcgattg cacgcccgta
    18661 ctcggcgaaa ttacctacgg catcgaacgc ttggcgatgt acctgcaagg cgtggaaaac
    18721 gtctatgacc tcgtttgggc aaaaacgccg gacggcaata ccgtaagcta cggcgacgtg
    18781 taccatcaaa acgaagtcga gcaatctacc tacaacttcg aatacagcga tgccgactgg
    18841 ctgctgcgcc agttcaacga ctacgaagcg caagccaaac gcctgttcgc cgaagaaaac
    18901 gccgaccttg ccttacctgc ctacgagctg gtcctcaaag cggggcatac gttcaacctt
    18961 ttagacgcac gcggcgcgat ttccgtaacc gagcgggcaa cttatattgg acgtattcgt
    19021 gcattgagcc gcgccgtggc gcaaaaatat atcgaaagcc gtgagaaact gggcttcccg
    19081 ttgattaaaa aataacgata cgtcgtctga aacaggtttt caggcgacct ttattgcgta
    19141 tccattaata caggccgtac ggcatatgca ggctttataa gcatggccta cggacaaaca
    19201 gaaagtctca catgacaacc caaacccttt taatcgaact ccttaccgaa gaactcccgc
    19261 caaaagccct gaataatctg ggcaaccatt ttgccgcttc cgttgccgaa ggcttggaaa
    19321 aagcgcaact ggttgacggc gcggccgaat ttacggctta tgcctcgccg cgccgtttgg
    19381 cggttcaagt caaaaacgtg aaggccgttc aagccgatca gaaaatcgtg aaaaaaggcc
    19441 ctgccgtggc gaatgccgta aaagacggtg cgccgaccaa ggctttggaa ggttttgcgc
    19501 gcggcgcagg cgcgaaaatc gaagacctga ccatcgtcca cgacggcagg caggacgtgt
    19561 acgcctatga atacgtccaa accggcaaac cgttgggcgg acttttggaa gacattatca
    19621 atcaagctgt taagaagctg ccgattccga aagtaatgcg ttggggcagc agcacgttta
    19681 ccttcgtgcg ccctgttcac gggctgattg tgctgcacgg cggcgatgtc gtgaacgtca
    19741 gcgttttggg cctgcaaagc ggcaatcaaa ccttgggaca ccgctttctt tccgacggcg
    19801 aaatcatcat tgaaaacgcc gacagctacg ccgcacaaat gcgcgggcaa ggcaaagtcg
    19861 tcgcttcgtt tgccgggcgc aaagccgcga ttcagacggc attggaaggg caggcacgcc
    19921 gtctgaacgc gaccgttgcc gccgatgaag ccctgttgga cgaagtaacc gcgctggtcg
    19981 aatggcctgt tgttttggaa gccggttttg aggaacactt cctcgccgtg cctcaagaat
    20041 gcttgatttt gacgatgcag caaaaccaaa aatacttccc gctgctcgac caaaacggca
    20101 agctgatgaa ccgcttcctg ctggtctcca acctgcaaac cgaaaacccg tcgcacatca
    20161 tccgaggcaa cgaacgcgtt ttgcgcgcgc gcctgtctga tgccgagttc ttctacaaac
    20221 aagaccaaaa agcgactttg gaaagccgcc tgcccaagct ggcgaacgtg gtgtaccaca
    20281 acaaaatcgg ttcgcaggcc gaacgcatcg aacgcctgca aagcatcgcc gcccatatcg
    20341 ccaaagcatt gggcgcggat gccgcggcag ccgggcgcgc cgcgcgtctt gccaaagccg
    20401 acttggtaac tgaaatggtc ggcgagttcc ccgaactgca aggcacgatg ggcaaatact
    20461 acgcccgctt ggacggcgaa accgaagaaa tcgccgaagc tattgagcaa cactaccaac
    20521 cgcgttttgc cggcgacaag ctgcccgaaa gcaaaattgc cgccgccgtg gcacttgccg
    20581 acaaactgga aaccctggtc ggcatttggg gcatcggttt gattcctacc ggcgacaaag
    20641 acccctacgc cctgcgccgc gccgccttgg gtattttgcg gatgctgatg cagtacggtt
    20701 tggacgtgaa cgaactgatt cagacggcat tcgacagctt cccgcaaggt ttgctcaacg
    20761 agaaaacgcc gtctgaaacc gccgacttta tgcaggcgcg ccttgccgtg ttgctgcaaa
    20821 acgattatcc gcaagacatc gttgccgccg tactcgccaa acagccgcgc cgtttggacg
    20881 atttgaccgc caaactgcaa gccgtcgccg tattcaaaca actgcccgaa gccgccgcgc
    20941 tcgccgccgc caacaaacgc gtgcaaaacc tgctgaaaaa agccgatgcc gcgttgggcg
    21001 aagtcaatga aagcctgctg caacaggacg aagaaaaagc cctgtacgct gccgcgcaag
    21061 gtttgcagcc gaaaattgcc gccgccgtcg ccgaaggcaa tttccgaacc gccttgtccg
    21121 aactggcttc cgtcaagccg caggttgatg ccttcttcga cggcgtgatg gtgatggcgg
    21181 aagatgccgc cgtaaaacaa aaccgcctga acctgctgaa ccgcttggca gagcagatga
    21241 acgcggtggc cgacatcgcg cttttgggcg agtaaccgtt gtacagtcca aatgccgtct
    21301 gaagccttca ggcggcatca aattatcggg agagtaaatt gcagccttta gtcagcgtat
    21361 tgatttgcgc ctacaacgta gaaaaatatt ttgcccaatc attagccgcc gtcgtgaatc
    21421 agacttggcg caacttggat attttgattg tcgatgacgg ctcgacagac ggcacacttg
    21481 ccattgccaa ggattttcaa aagcgggaca gccgtatcaa aatccttgca caagctcaaa
    21541 at
//
LOCUS       DAXFZR010000033        21321 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000033 DAXFZR010000000
VERSION     DAXFZR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21321
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.033"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(60..779)
                     /gene="pyrH"
                     /locus_tag="ACFJAS_001042"
     CDS             complement(60..779)
                     /gene="pyrH"
                     /locus_tag="ACFJAS_001042"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC3982319.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(996..1850)
                     /gene="tsf"
                     /locus_tag="ACFJAS_001043"
     CDS             complement(996..1850)
                     /gene="tsf"
                     /locus_tag="ACFJAS_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC3982320.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFIRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(1979..2707)
                     /gene="rpsB"
                     /locus_tag="ACFJAS_001044"
     CDS             complement(1979..2707)
                     /gene="rpsB"
                     /locus_tag="ACFJAS_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC3982321.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(2880..3113)
                     /locus_tag="ACFJAS_001045"
     CDS             complement(2880..3113)
                     /locus_tag="ACFJAS_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982322.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            3191..3772
                     /locus_tag="ACFJAS_001046"
     CDS             3191..3772
                     /locus_tag="ACFJAS_001046"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC3982323.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKKGR
                     KIGVYWPMGNELRLDGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            3833..4423
                     /locus_tag="ACFJAS_001047"
     CDS             3833..4423
                     /locus_tag="ACFJAS_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC3982324.1"
                     /translation="MSERIILSVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            4807..6273
                     /locus_tag="ACFJAS_001048"
     CDS             4807..6273
                     /locus_tag="ACFJAS_001048"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC3982325.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGADGVINPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCRYLQKRYDVFKTQKLFENMEFSTDRNKISDWAPLIMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDWQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGLPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSFMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(6460..6831)
                     /locus_tag="ACFJAS_001049"
     CDS             complement(6460..6831)
                     /locus_tag="ACFJAS_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC3982326.1"
                     /translation="MKLLTTAILSSAIALSSMAAAGTDNPTVAKKTVSYVCQQGKKVK
                     VTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSYR
                     KQPIMITAPDNQIVFKDCSPR"
     gene            complement(7069..7368)
                     /locus_tag="ACFJAS_001050"
     CDS             complement(7069..7368)
                     /locus_tag="ACFJAS_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3982327.1"
                     /translation="MKIFENIEDVKAIRKKTGMNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECLDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKAERKKQG"
     gene            complement(7467..8246)
                     /gene="map"
                     /locus_tag="ACFJAS_001051"
     CDS             complement(7467..8246)
                     /gene="map"
                     /locus_tag="ACFJAS_001051"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC3982328.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            complement(8286..8936)
                     /locus_tag="ACFJAS_001052"
     CDS             complement(8286..8936)
                     /locus_tag="ACFJAS_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982329.1"
                     /translation="MPFEPPSDGIARHPKSTIKMAKKPNKPFRPTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPSPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNAPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
     gene            complement(8944..9552)
                     /locus_tag="ACFJAS_001053"
     CDS             complement(8944..9552)
                     /locus_tag="ACFJAS_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC3982330.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(9616..10209)
                     /locus_tag="ACFJAS_001054"
     CDS             complement(9616..10209)
                     /locus_tag="ACFJAS_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC3982331.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(10214..10561)
                     /locus_tag="ACFJAS_001055"
     CDS             complement(10214..10561)
                     /locus_tag="ACFJAS_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC3982332.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            10608..11483
                     /gene="rsmI"
                     /locus_tag="ACFJAS_001056"
     CDS             10608..11483
                     /gene="rsmI"
                     /locus_tag="ACFJAS_001056"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC3982333.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(11518..12048)
                     /locus_tag="ACFJAS_001057"
     CDS             complement(11518..12048)
                     /locus_tag="ACFJAS_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC3982334.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            12327..13481
                     /gene="obgE"
                     /locus_tag="ACFJAS_001058"
     CDS             12327..13481
                     /gene="obgE"
                     /locus_tag="ACFJAS_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC3982335.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            13535..14482
                     /locus_tag="ACFJAS_001059"
     CDS             13535..14482
                     /locus_tag="ACFJAS_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3982336.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIILETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            14479..15531
                     /locus_tag="ACFJAS_001060"
     CDS             14479..15531
                     /locus_tag="ACFJAS_001060"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFC3982337.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFPEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            15547..16968
                     /gene="cysS"
                     /locus_tag="ACFJAS_001061"
     CDS             15547..16968
                     /gene="cysS"
                     /locus_tag="ACFJAS_001061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC3982338.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGRSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            17139..17918
                     /locus_tag="ACFJAS_001062"
     CDS             17139..17918
                     /locus_tag="ACFJAS_001062"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC3982339.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            17905..18249
                     /locus_tag="ACFJAS_001063"
     CDS             17905..18249
                     /locus_tag="ACFJAS_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982340.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            18259..18789
                     /locus_tag="ACFJAS_001064"
     CDS             18259..18789
                     /locus_tag="ACFJAS_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC3982341.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(18882..19997)
                     /gene="asd"
                     /locus_tag="ACFJAS_001065"
     CDS             complement(18882..19997)
                     /gene="asd"
                     /locus_tag="ACFJAS_001065"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC3982342.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNIIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            20172..20753
                     /locus_tag="ACFJAS_001066"
     CDS             20172..20753
                     /locus_tag="ACFJAS_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC3982343.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(20815..>21321)
                     /locus_tag="ACFJAS_001067"
     CDS             complement(20815..>21321)
                     /locus_tag="ACFJAS_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFC3982344.1"
                     /translation="DKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
BASE COUNT         4766 a         5284 c         6041 g         5230 t
ORIGIN      
        1 aaatggctgt ctgaagccca tatgcgacta tatctgccga ccctatggtc aatcatcaat
       61 cagcagtgga ccagtgtgcc ttcgtcttcg ccggtaatga cgcgtttgag cgagccttgt
      121 ttggcgatgc cgaagacgac gatattgagc ttgcgttcgc ggcagagggc aaaggcggtc
      181 gcgtccatga ctttgaggtt tttattcaag gcttcgtcaa aagtaatggt ttcgtagcgt
      241 gtggcggacg ggtctttttt cgggtctgcg gtgtacacgc cgtcgacgtt ggtggctttg
      301 agcatcacgt cgcagttcat ttccgcgccg cgcaatgcgg cggcagtgtc ggtcgtgaag
      361 aacggattac cggtaccggc ggcgaaaatc acgactttgc cttcttccaa atattgaatg
      421 gctttaggac gggcgtaagt ttcagcgatt tgctgcatag acagtgcgga ttgtacgcgc
      481 gctttgatgc ccaaggtttc aaatgcgtct ttgagtgcca aggcgttcat cacggtcgcc
      541 atcatgccca tgtagtcggc agtggcgcgg tccatgctgc ctgcttgtgc agatacgccg
      601 cggaaaatgt taccgccgcc gacaacgata ccgacttgca cacccatttt cacgatttcg
      661 gcaatttcgc cgacagtttg aacaatggta tcgtgattga tgccgaacgg atcggaaccc
      721 atcagggatt cgccggagag tttcagtaat acgcgtttgt atttgatttg ctgtgtcatg
      781 ggataccttg ctttcttgaa tgtcgtcggt cggagttggg acgtctgaat ttcaaatggt
      841 cggattacaa tgatatggtg tgtcgttcta ttattgtttt gttttcagac ggcctttata
      901 ttcggtcgtc ttaaaatagg taccgtaaac ggttttctca aaaaaaagca ccctgattcg
      961 tttggaatcc aggtgctttc tttttcataa gtgccttaca ctttggcggc tgcggctact
     1021 tcggctgcgt agtcgacaac ggctttctcg ataccgtcgc ctactttgta gcggatgaag
     1081 ctaaccactt cagtgtcgtt ttctttagcg aattgggcaa cagtttggtc agggttcatc
     1141 acgaatgctt ggccgttcag agtgatttca gccaagaatt tgcggatacg gccttcaacc
     1201 attttagcgg cgatgtcggc aggtttgccg gaagcgatgg cttgctcggt gtagatgtgg
     1261 cgttcttttt caacggtttc agcatctact tcggcttcgc ttacgcattg cggtttggcg
     1321 gcaacgatgt gcataccgat tttgcgtgct acatcttcag agcctttgta ctcgaccaaa
     1381 acgccttcgg tcgccaatgc gccgtggatg taggcaacca gttggttggc agtgtcaatc
     1441 acttggaagc ggcggacaga catgttctcg cccaatttag cgatgatggc tttgcgttct
     1501 gattcaacca gttcgctcag ttcttcaaca gaagccggtt ttttctcggc agcagttttc
     1561 gcaacgaagt tggcaaattc tacgaagccg gcgtctttag caacgaagtc ggtttcgcag
     1621 tttacttcga ccaatgcgcc gacattaccg ttgattgcat aagccaatac gccttcggca
     1681 gcagtacgac cagccagttt gcccgctttc gcaccggatt taatacgcag gatttcttcg
     1741 gctttgtcga agttgccttc agcttcaacc aaggcttttt tgcattccat catacccagg
     1801 ccggtagcgg cgcgcaggtc ggcaaccatt tttgcagtaa tttctgccat tttgaatctc
     1861 ctagattttt gggaaatacg gaaaggattt tccacattcg ggaatcgtgt gccgcgaacg
     1921 ggcgttcaga cggcatattt gagaaaaggg gcataacgcc cctcttctgt gtaccgaatt
     1981 actcggcagc ggtttcttgg gcagcggcta cggtttcttg cagcgcttgg tttttgcctt
     2041 ccaaaactgc gtcggcgatg cctcggcagt acaggcggat ggctttggcg gagtcgtcgt
     2101 tgccggggat gacgtatttc acgccgtctg ggctgttgtt ggtatcgact acggcgataa
     2161 cggggatgcc cagtttttca gcctcaacca aagtaccttt ttggtagccg gtatcgataa
     2221 cgaaaatcgc gtcgggcagg cctttcatgt ttttgatacc gcccaaagag cgttccagtt
     2281 tttcaacgtc gcgttgcatt tccagaattt cttttttgct gaaaccgctt tcggcagcat
     2341 tttccaaggc tgcggttttt tcttccaggc gtttgatgga ttgcttaacg gttttgtagt
     2401 tggtcagcat accgcccaac cagcggtggt cgacgaaagg cataccggcg cgggtcgctt
     2461 cttcgcggat gatgtcgcgt gcctggcgtt tggtacctac gaacaatacc gtacctttgt
     2521 tggcaaccag acgacgtacg gcttcttgcg cctcttggaa catcggcagg gttttttcca
     2581 agtttacgat atggattttg ttgcgcgcgc cgaaaatgta ttgagccatt ttcgggttcc
     2641 agaaacgggt ttggtgaccg aagtgaacac cggcttcaat catctgacgc atagtaattt
     2701 gagacatgtt atttccttga aagggttaaa gagcacacat tcaatcgcat agaacttgat
     2761 aggcggcgcc tattgtcaag cacccttcgg cgaaagtggg cataagtttt aaaaatagtg
     2821 tgtttccgaa atggaaaact gacggattat agcggataag atgccgtctg aaaagggatt
     2881 cagggttttg tgccgctgtc tttttgccgt tgtgcgcgct gctgttcgga ttctgcctgt
     2941 tcctgctgcc tcatcaggcg gttgcggcgg cggacggtta cggcgaagac cgtgaacacg
     3001 gtgggcagaa ccacccaaaa aaccaaataa atcaaagcgc gcgcaatact cggctgcgcg
     3061 gcggaataca taacggcaac aaaaagatag ccgatggcga cgatgtgcca cattgtgtgc
     3121 gtcctgtttg aatgttaaca aaatcgttat aatcgcaaca ttctaccgca atcctgaaag
     3181 gccgtctgaa atgaggaacg aggaaaaacg cgccctgcgc cgcgaattgc gcgggcggcg
     3241 ttcgcaaatg gggcgggacg tgcgggcggc ggcggcggta aaaatcaacc gcctgctcaa
     3301 acgttatatc aaaaaggggc ggaagatcgg cgtgtattgg ccgatgggca acgagctgcg
     3361 tttggacggc tttgtccgcg cggcgcaaaa acgcggcgca aaactctatc tgccttatat
     3421 cgaaccgcac acgcggcgga tgtggtttac gccgtatcct gaacgcggaa tggaacggga
     3481 acgcaagcgc ggtagggcga agctgcatgt ccctcagttt gcagggcgca aaatccgcgt
     3541 gcacggtttg tcggtattgc tcgtcccgct tgtcggcata gaccgcgaag gctaccgttt
     3601 ggggcaggca ggcggctatt acgatgcgac gctttcggcg atgaaatacc gtttgcaggc
     3661 gaaaaccgtg ggcgtgggct ttgcctgcca gttggtggac aggctcccac gcgaggcgca
     3721 cgacctgccg ctggacggtt ttgtatcgga agcggggata ttgtgttttt agccgttcag
     3781 acggcatttc gggcggaaat gccgtctgaa ggcggaatat tgggagaaaa ggatgtcgga
     3841 aaggataata ctgtcggtct tgtcgttggg cggcgtacgg ctcgaaccgt tggatgtgca
     3901 tcatgaaacg ggtttgcgcg aggcggtttg cgatggggag gtttggaagc tgggggtaac
     3961 gtccgcgccc catccggata gggtggcaga ctatatcggg acagcattgg caacgcgttt
     4021 ggcgtttgcg gttgtcgatg aagaggcgga cagggtggtc gggacaacgg cgtattatca
     4081 ttttgaaccg cagattccgc gtttggatat cggattcaca tggtatgcgg catcggcacg
     4141 gcggaaacgg ataaacacct gttgcaaaat catgctgttg gattacgcat ttgatgtttt
     4201 agcttgctgt tgcgcgggat ggcgcacgga tattctgaac cttgcctcgc aacgtgccat
     4261 cgaacggctg ggcgcggaaa aagacggcgt gctgcgtatg catatgctcc gtaaagacgg
     4321 cagtgtgcgc gatacggttg tgtacagtat gctgagggaa gattggtgca aaaacaggga
     4381 aatcctgacc ggcaggttgg cagggtatgg cgtgcaggtg tgaggcagta ttctcaaatt
     4441 taaaaaactt aacgcgatat gctgacaatc ttttaaaaat taatatataa acaattttga
     4501 ttttttgtct attaaaaatt tgatatatat agataaataa ctatttttat tttttgtttc
     4561 gcagttcccc ggcttggcgg gcgacagaaa aactttgcat ttcccagtaa aatccactac
     4621 attcacgata acatattaca agcccctgaa gcggtgtcgg aagccatact tgcaacgcat
     4681 tccaaccccg aaacagcgtg ctcggacaca caccattgcc ggtttcccct gcttttgcga
     4741 ttcaggcgtt tgtagtacgg caaataacca taagggcaga agatgcctgt tttttaggag
     4801 taaaccatgg ctgaagcgac agacgttgtc ttggtgggcg gcggcattat gagcgcgact
     4861 ttgggcgttt tgctcaaaga actcgaaccg tcttgggaaa tcaccctgat tgaacgcttg
     4921 gaagatgtgg cgttggaatc gtcaaacgcg tggaacaacg ccggcacggg gcattccgcg
     4981 ctgtgcgaat tgaactatgc gccgctgggt gcggacggcg tcatcaatcc ggcgcgcgcc
     5041 ctcaatattg ccgaacagtt tcatgtcagc cgccagtttt gggcgacgct ggtcgcggaa
     5101 ggcaagttgg aagacaattc cttcatcaat gccgtgccgc atatgtcttt ggtgatgaac
     5161 gaagaccact gccgttacct gcaaaaacgc tatgatgtgt ttaaaacgca gaaacttttt
     5221 gaaaatatgg aattttccac cgatcggaac aaaatttccg attgggctcc gctgattatg
     5281 cgcggccggg acgaaaacca acccgtcgcc gccaactatt ccgccgaagg cacggatgtc
     5341 gatttcggac ggctgacgcg ccagatggtg aaatatttgc agggcaaggg cgtaaaaacc
     5401 gagttcaacc gccacgtcga agacatcaaa cgcgaatccg acggcgcgtg ggtgctcaaa
     5461 accgccgata cccgcaaccc agactggcag ctcaccctcc gcacccgctt cctcttcctc
     5521 ggcgcgggcg gcggcgcgct gaccctgctg caaaaatccg gcatccccga aggcaaaggc
     5581 tacggcggct tacccgtgtc cggcctgttc ttccgcaaca gcaaccccga aaccgccgaa
     5641 caacacaacg ccaaagtgta cgggcaggct tccgtcggcg cgccgccgat gtccgtcccg
     5701 cacctcgaca cacgcaacgt agacggcaaa cgacacctta tgttcggtcc ttacgcaggt
     5761 ttccgttcca acttcctcaa gcaaggctcg tttatggatt tgccgctgtc catccatatg
     5821 gacaacctct atcctatgct gcgcgccggc tgggcgaata tgccgctgac caaatacctg
     5881 ctgggcgaat tgcgtaaaac caaagaagaa cgctttgcct ccctgctgga atactacccc
     5941 gaggcaaacc ccgacgactg ggaactcatc accgcagggc aacgcgtcca aatcattaaa
     6001 aaagactccg aaaaaggcgg cgtgctgcag tttggtacgg agattgtcgc ccacgccgac
     6061 ggctcgctcg ccgcattgct gggagcgtcg ccgggcgcat cgaccgctgt gccgctgatg
     6121 atccggctga tgcaccaatg cttccccgag cgcgccccgt cttgggaagg tcgtctgaaa
     6181 gagctggtac cgggttacgg catcaagttg aacgaaaacc ctgaaagggc ggatgaaatt
     6241 atcgcctata ccgcgaaagt gttggatatt taaaccgaat ctttcaagaa aagccgtacc
     6301 cccatatggt gttgacaccg tccctgccgg ttatgccctt catccggcag ggcaggtttt
     6361 gatacacggg gcaggtaatc gttttttccc ttttgcgaat aatggaacgg tcaaaaaaca
     6421 gcgttttcag aaatgaaaac gctgtttttt gttgcctgat taacgtgggg aacagtcttt
     6481 gaagacgatt tggttgtcag gtgcggtaat cataataggc tgtttgcggt aggatttgct
     6541 gtccattgcg ccggtgctca gcacataacc gccttctttg ccgtagaacg tgtccatatt
     6601 gtcggattta tccaaattga tgggcatttg cacacgtttg ccgttgatga cggcggaggc
     6661 gtatgtggtc agaccctgtt tgttgaagcc gtaggttact ttgacttttt taccttgctg
     6721 gcagacgtag ctgacggttt ttttggcaac ggtggggttg tccgtgccgg cggcggccat
     6781 actgctgagc gcgattgcgg aagacaggat tgcagtggtc agaagtttca tttttattcc
     6841 tttggataga tgtggagttt gcccgtttcg gactgtttga cagtctaatg tcggcgtatc
     6901 ctgaaataaa caggaaattg gataaagttt gtaaaaaggc aggttcgggc ggttcggcgg
     6961 tttgaggcag gggcggcagg cgggatacgg cggcctgtcc ggtgtttcag acggcattgt
     7021 ttgcattttt cggaaatgaa aaacctccgg gcatccggag gttgcggttt aaccttgttt
     7081 ttttctttcg gccttggtct gtttggacaa ttcggcatac aggtcgggat ggtgtttttt
     7141 caacagggcg gcgatttcca tatctttttt gttgactttt gccaaatcga ggcattcgat
     7201 atggacgagg cggagcagtt cgcgtacggg tttgggcatc ttgcggccgg tttcgtagcg
     7261 tgaacctccg gattgagtaa cgccgacctt gccccagaag tctatctggt tcatcccggt
     7321 ctttttacgg atggctttaa catcttctat attttcaaat attttcataa aacttccttt
     7381 aagtttttct ggttgcgggg caaattccga tttgctttgt tttcgtaatg tgccgggtca
     7441 agcctgtttt tatattgcat acagcttcag ggtttgccgg tggcggggct gacggtgagg
     7501 atttcgtagc cggtttcggt taccaagact tcgtgttccc attgggcgga gagggagcgg
     7561 tctttggtaa ccaccgtcca gccgtcgttg aggatacgca ggtggcgttt gccttggttg
     7621 atcatcggtt cgacggtaaa aatcataccc ggttttagaa cgggaccctg cccttttttt
     7681 ccgtagtgca acacttgcgg ggcttcgtgg aaaccgcgcc cgatgccgtg tccgcagaat
     7741 tcttgtacaa ccgaataacc tgcattttcg gcaacctgct ggcaggcgta acctacgccg
     7801 cccagcgtcg cgccaggttt gacggcttct atgcccgcca tcatagaggc gtgggttacg
     7861 tcgatcaggc gttgggcgat gggggagact ttgccgacgg taaacatacg gctggagtcg
     7921 ccgtggaaac cgtctttttt gatggtgagg tcgatgttga taatgtcacc ttctttgagc
     7981 ggcttgtcgt cgggaatgcc gtggcagatg acgtggttga cggaggtgca gcaggatttg
     8041 gggtagggcg ggttgccgta gtggaggggg gcgggatagc cgccttggac gttgacgtgg
     8101 tagtcgtaaa caagtttgtc gatttcgtcg gtggtgatgc cgggtttgac gaattgtccg
     8161 atgtagtcga gggcttcggc gacgagtttg cccagctcgc gcattttttc gatttcttcg
     8221 ggggttttga tgatgatgcc gttcataatg cttccttgga ttgcgggtgt gcctgccgtg
     8281 ccgggtcaaa agaggttgtc gagaatctct ttatggggtt tggccggcgt gtttttcggg
     8341 gtgtccggtt tggtatggtt ttctttgggc gtttcttttt ctttgggtgt ttctttgggc
     8401 tgcgcctgtt tttcgggtgt ttcttttcgg ggttcgggat ggggggcgtt tgtgggcagt
     8461 acgggggttt ctttgagtgt gttttcagct tgtgttccgg tgccggttaa cgaatcggct
     8521 gcccgggccg catccgaagg gcgcggtttg acccggtttt tcttgggtgc ggcaggggac
     8581 ggcagtccgc tgccgctttc ttctgtgccg tccggctgtg cgggttcgga tgaggcggcg
     8641 tggtggtgtt cgggttgggc ggcatcttgt ccgacggcgc cgtttggcag ccagaattcg
     8701 gtttcgcggg ggctgtcggt gtgttgcggt tcggtttgga gggttttgtc gccgttcggg
     8761 ttgaatgtgc tgacgatgcc tactgccaat gcgccgatgg cggtgataca gatgagcaat
     8821 acggcgcgta tcaggagttt gggggtcggc ctgaagggtt tgttcggttt ttttgccatt
     8881 ttgattgtgc ttttggggtg tcgggcaatg ccgtctgaag gcggttcaaa cggcattgcc
     8941 gagtcagcgt tggacgtagt tttggtagag ggtaatgact ttttgtacgc cgacggtggt
     9001 gctgactttt tgggtaatct gcgcctgttc ttcgggggtg aggatgccca taacgtaggt
     9061 tacattgccg taggtaatga ttttgacgcg cgcctgtgta gcggggctga tgcccagcag
     9121 cgtggcgcgg actttggacg tgttccaagt gtcgccggcg atgtcgcccg cagtgcgcgg
     9181 cagggaggcg acggtaatgt agttgtatac gccttcggcg gcctgttcgg aacgtgcaat
     9241 ctgaccgacg aactgttttt cgccttcggt ggcgacttgt ccgagcagca gcaggtggcg
     9301 gttgtagccg acgacggaga tttggggcgt gtagcctttg gtttggttgt tttggcgcag
     9361 gtaggaacgg gcggtggttt cgatacgcaa cgccataacg ttgtcatcgg tttgcgcgcc
     9421 ggtggttcgg cggtcgatga cggatttcgc gccgacggcg gccccgccga cgactgcgct
     9481 gaagcagccg ccgagggcaa ggctgaggac ggcggcaatc agggtgcgga cggtgtgtgg
     9541 tttgggtttc atcggggact tcctttcttg ggcgtttcag acggcattgc tttgcgccat
     9601 gccgtctgaa aagggttaca ttccttccag cagtacggag tcgatacagt cgcacatggc
     9661 gtgtatcagc aggatgtggt tttcctgaat gcgggcggtg cgcggatggg ggacgttgag
     9721 cagaacgtcg gtgtctttga gcatggcggc gattttgccg ccgtcgcggc cggtcagggc
     9781 gatgacgtgc atatcgcgtt cgtgcgcggc tttgacggct tcgatgacgt tggcggaatt
     9841 gccggaggtg gaaatgccga cgaggacgtc gcctgcacgt ccgagcgcgc gcacttgttt
     9901 gctgaatacg tggtcgaaac cgtagtcgtt gccgatggcg gtcagcgcgg aggtgtctgt
     9961 ggtcagggcg acggcggcga gttccatgcg ttctttctca aaacgcccgg tcatttcggc
    10021 ggcgaagtgt tgcgcgtcgg cagccgaacc gccgttgccg caggccagga ttttgccgtc
    10081 gttcatcagg cattgcagca tcagctcggc agcctgtacg gtcggctcga ccagtatttt
    10141 ttcggcttcc tgtttggcac ggatgctttc ggcaaaatgt gcggcaacgc gttcttgtaa
    10201 tgtcgtcata tcgtcaacct gtaatattct ttatccactc gggcgggcgg tttccttcga
    10261 taagtaccgc atcgaggcgg cacggtacgt ttgtcagcct gttctgttgc agataatact
    10321 ctacacttcg ttgcagtttc aataatttgg acggagaaat gctgtatgcc gcgccgccga
    10381 accgttggtt tttgcggtat tttacttcaa caaacagaat catgccgccg tttttgacaa
    10441 tcaggtcgat ttcgccgtag gcgcagtgcc agttgcgggc aagcagcgtg cagccttggg
    10501 attggaggaa ggcaagtgcg gcatcttccc ctgccgtacc ctgtttgtgg tttaagcgca
    10561 taatcggttt aacctttata atgtctgttt ttcagacggc attttttatg tttcagaaac
    10621 acttgcagaa agcctccgac agcgtcgtcg gagggacatt atacgtggtt gccacgccca
    10681 tcggcaattt ggcagacatt accctgcgcg ctttggcggt attgcaaaag gcggacatca
    10741 tttgtgccga agacacgcgc gttactgcgc agcttttgag cgcgtacggc attcagggca
    10801 ggttggtcag tgtgcgcgaa cacaacgagc ggcagatggc ggacaaggta atcggtttcc
    10861 tttcagacgg cctggttgtg gcgcaggttt ccgatgcggg tacgccggcc gtgtgcgacc
    10921 cgggcgcgaa actcgcccgc cgcgtgcgcg aagcagggtt caaagtcgtt cccgtcgtgg
    10981 gcgcaagcgc ggtaatggcg gcgttgagtg tggccggtgt ggcggaatcc gatttttatt
    11041 tcaacggttt tgtaccgccg aaatcgggcg aacgtaggaa attgtttgcc aaatgggtgc
    11101 gggcggcatt tcctgtcgtc atgtttgaaa cgccgcaccg aatcggggca acgcttgccg
    11161 atatggcgga attgttcccc gaacgccgtc tgatgctggc gcgcgaaatc acgaaaacgt
    11221 ttgaaacgtt cttaagcggc acggttgggg aaattcagac ggcattggcg gcggacggca
    11281 accaatcgcg cggcgagatg gtgttggtgc tttatccggc gcaggatgaa aaacacgaag
    11341 gcttgtccga gtctgcgcaa aatgcgatga aaatccttgc ggccgagctg ccgaccaagc
    11401 aggcggcgga gcttgccgcc aagattacag gtgagggcaa aaaggctttg tacgatttgg
    11461 cactgtcgtg gaaaaacaaa tgatgccgga tggaccggca ccatagtgcg aacggaatca
    11521 acggacacgc cccgtcatgc ggtacagcac gtctttgcct tgaacacggt ggacgacgac
    11581 catggcgacg tgtccgacga cggcggcaaa cagcagccag cccaaattgc cgtggaacgt
    11641 gttgcccaag tttgccatcc actcgatttt ttccggcgaa ccctgcatta cttcaacgcc
    11701 gaacactttc aacgggccgc gtccgccacc gtattggcgg atcatgccga taacgggaac
    11761 ggcaagcatg agcagataca gaatgccgtg tcctgccgct gccgccttgc tgtcgctttg
    11821 cggacgcttg gcgcggttgg caaccgccca cacgatgcgc aatgcaatca ccgtcagcat
    11881 gaggaaaccg aaagatttgt gcaggccgaa caggctgccc gcccattccg cctcttcgta
    11941 aattgtccac aggacaatgg tggtcagaat gccgatgaag ccggcaacgg tcagccagtg
    12001 cagcgcgcgt gtcaacgggt cgtaaacggc aggttggttt gtgttcataa aaaaatcctt
    12061 atcatatgta acggtttgaa cggacattcc gtcgggtcgg aatgtcaaaa aggcgcgatt
    12121 gtaccaaaga agttggacat attttgtttg caggctgaag atttgcttaa aaattcatta
    12181 agatgggcag aacaaatagt ttctccgcgg tatattgaaa taccgcttat accttataat
    12241 gtcggcagaa aatctgttca gacggcatca aaacatatgg caatccgcct gaaaccctat
    12301 ttaaaaccaa caaacaagag catttaatga aattcatcga cgaagcaaaa atcgaagtcg
    12361 ccgcaggcaa aggcggtaat ggcgcaacca gtttccgccg cgaaaaattc gtaccccgcg
    12421 gcggcccgga cggtggcgac ggcggcaaag gcggcagcgt ctgggcagaa gccgacgaaa
    12481 acaccaacac cctcgtcgaa taccgcttcg tcaaacgcta ccaagccaaa aacggcgaaa
    12541 aaggccacgg ttccgaccgc tacggcgcag gtgcggacga cattgtcctc aaaatgcccg
    12601 tcggcaccct tatccgcgac ctcgacaccg acgaaatcgt tgccgacctc acctaccacg
    12661 gacagcgcgt ctgcctcgcc aaaggcggca aaggcggctt gggcaacatc cacttcaaat
    12721 cctccgtcaa ccgcgcccca aaacaatcca cgcccggcga agaaggcgaa acccgttccc
    12781 tgcaactcga actcaaagtc ctcgccgatg tcggcttgtt aggcatgccc aacgccggca
    12841 aatccaccct gattaccgcc gtctccgccg cacgccccaa aatcgccaac taccccttca
    12901 ccaccctgca tccgaacttg ggcgtggtac gcatcgacga aaaccacagc ttcgtgatgg
    12961 ccgacatccc cggcctgatt gaaggcgcgg cagaaggcgc aggcctcggc caccgcttcc
    13021 tcaaacacct atcacgcacc ggtctgctgt tacacgttgt cgatttggca cccttcgacg
    13081 aaaccgtcaa tcccgccgaa gaagcactcg ccatcatcaa cgaattacgc aaatacgacg
    13141 aagaacttta cggcaaaccg cgctggctgg tgctgaacaa actcgatatg ctcgacgaag
    13201 aagaagcccg ggcgcgaaca gccgccttcc tcgaagccgt cggctgggac taccccgaac
    13261 ctgatgaccg tttccaattc gacatggaaa ccccgcgcct cttccaaatc agcgcgctga
    13321 cacatcaagg cacgcaggag ttggtacacc aaatcaacca atacctggcc gagaaaaaac
    13381 gtatcgaggc tgaaaaagcc gaagcggaga aagcagcggc aaatgttgaa attatcgaac
    13441 agcagcctaa aacggatact ggcgtgttta agccggagta aaaggacaag gccgtctgaa
    13501 agttttcagg cggctcattc tgcagagatg tgctatgtac aaaacaatag atttattttc
    13561 aggcattggc ggaatacgcc taggctttga aaaatacggg tgcaccaatg tattctcatc
    13621 ggaatgggac aaatatgccc gacaagtgta tgaagcaaac tttggagaaa aaccttttgg
    13681 cgacattaac ggcatcgatc cttcagatat tcccgatcat gatattctgc ttgcgggatt
    13741 tccgtgccaa cctttcagta tcgcaggtaa gggattggga tttgaagata cgcgcggaac
    13801 attgtttttc aatattgccg aaatcctgaa aaccaaacag ccgaaggctt ttcttctgga
    13861 aaacgttaag cggctgacta cgcacgattc gggcaggact ttccgtatta ttttagaaac
    13921 ccttaaacag cttggttata cggtttattt taaggtttta aatactttgg atttcggatt
    13981 gccgcaaaaa cgcgagagaa tttatattgt gggtttttca gacaacatcc ctttttactt
    14041 tcccgaaccc ataaaccaat atcggccatt gggcgaactg ctggaaaacg atcgagatgt
    14101 cgaaccaagc tattttttat cagatacctt gaaacaaaaa cgtttggcag ccttaaaaaa
    14161 agctccgccc accccatcaa tttggcatga aaatattggc gggaatgttt ccgcacttcc
    14221 atattcctgt gccttaaggg ccgggggaag ttacaattat ttggtggtaa acggagtaag
    14281 aaggctaaca ggaagggaaa tgttaagatt acaaggtttt cccgatgatt ttgaaatcaa
    14341 tatcccctac tcgcaagtca ggaaagtcgc cggaaattct gtatccgtcc ctgtaataga
    14401 agccatcgcg gaaaatatgc tcgcttctct ttccggcaag gtcgaacaaa aagggcaatt
    14461 ggatttattg gaggcaggat gacgcttgaa gaacaacaag ccaaagaagc attggacggc
    14521 attatcaaaa aatcccgtgt ccacctttat aaacccattc aaattgcaga aattttatat
    14581 catgaccgtt gtataaaaca gttggatttt ttaaatttag atacttaccg caaccaatct
    14641 aaacgctgga gggatgaaat ctgccgtcgg tttttagggc ggatttctac ttcatctgca
    14701 aaattccaag ataatctgtt tgaaaaaaat gcaataccgc ctgaaaaact agcggtattg
    14761 ggaacattaa acagacagtc ggacggcggg gtagaatcgt acatttataa acagtttttc
    14821 aatcgttttt cccaaatgag cgaagcattg gcttatgtcg gcaatacaga cagatactcc
    14881 ttccaactgt ctgaatttct aaatttattt tggctcgagc cgggattgaa aagaagtata
    14941 gacaagatat atgaaatcgt tgtttatgcg ttgtttgatg cattggtttc agaattaggc
    15001 ataacggttt caattgattt tcctaaggaa aacctgtttt tatgggagga atatcaagat
    15061 tttcccgaaa aaatcatcac tatgccgaaa aatgagcatt taaaacttcc tgcaaaaatc
    15121 catcgtgtag gcgtaaccaa tgctgccgat agagggttgg atatgtggtc taacttcgga
    15181 ttggccatac aggtcaaaca tctctcttta gacgaagagt tggcagaaga tattgtatcc
    15241 tccattagcg cagatagaat tgtcattgtc tgcaaaaagg cagaacaatc ggtgattgtt
    15301 tcattactga cgcaaatagg ctggaaaagc cgaattcaaa acatcgtaac tgaggatgat
    15361 ttgataagtt ggtacgaaaa agcattgaga ggccaatatc cgattgcaga agcgttgttg
    15421 gaaaatatta aaactgaaat tatgcgtgaa tttcctgccg ttaatgaagc caatgaattt
    15481 ttagatttcg cccaaaatcg cggatatgac attactgtta ctcatttcta aacttacttg
    15541 atgaatatga ccgccatcta caacaccctt acccgtcaaa aagaaccctt cgcccccatc
    15601 gaccctgaaa acgtgcgtat gtacgtctgc ggcatgaccg tttacgacta ttgccactta
    15661 ggccatgccc gtgtgatggt ggtgttcgac atgattgccc gctggttgcg cgagtgcggt
    15721 tatccgctca cttatgtgcg caacatcacc gacatcgacg acaaaatcat cgcccgtgcg
    15781 gctgaaaacg gcgaaaccat cggcgaactg aacgcgcgtt tcattcaggc gatgcacgaa
    15841 gatgccgatg ctttgggcgt gttgcgtccc gacatcgagc cgaaggcgac ggaaaacatc
    15901 ccgcaaatga ttgccatgat tgagaccctg attcaaaacg gcaaggcata tcctgccgca
    15961 aacggcgacg tttactacgc cgtgcgcgag tttgccgctt acggacaatt atcgggcaaa
    16021 tcgttggacg acctgcgtgc gggcgaacgc gtggaagtgg acggtttcaa acgcgatccg
    16081 cttgattttg tattgtggaa agcggcgaaa gcaggcgaac cggcgtggga aagcccgtgg
    16141 ggcaacggcc gtccgggttg gcacatcgaa tgttccgcga tgagtgaaaa cctgttcggc
    16201 gatacgttcg acattcacgg cggcggcgcg gatttgcagt tcccgcacca tgaaaatgaa
    16261 atcgcccaaa gcgtcggtgc cagcggtcac acctgcggtc atgaccacgc gcaaacccac
    16321 cacggtcgaa gtattgccag ccacgtcaaa tactggctgc acaacggctt catccgtgtg
    16381 gacggcgaaa aaatgtccaa atcgctgggc aacttcttta ctatccgcga agtattgaaa
    16441 caatacgacc ccgaagtcgt gcgtttcttc atcctgcgcg cccactaccg cagcccgttg
    16501 aactactccg acgcgcattt ggacgacgcg aaaggcgcgt tgacccgtct gtacaccacc
    16561 ttgaaaaaca caccggcggc tgagtttgat ttgtctgaaa acgccaacgg ctacacccgc
    16621 cgtttctacg ccgccatgaa cgacgatttc ggtacggtcg aagcggttgc cgtgttgttt
    16681 gagctggcag gcgaagtgaa caaaaccaat gacgcacacc tcgccggctg cctgaaagct
    16741 ttgggcggca tcatcggcct gctgcaacgc aacccgattg agttcctcca aggcggcgca
    16801 gtttcagacg gcctctccaa cgaagaaatc gaagacttga ttgcccgacg caaacaggct
    16861 cgcgcggata aaaactgggc ggagtccgac cgcatccgcg accttctgaa cgaacacaaa
    16921 atcattttgg aagacagcgc cggcggcacg acttggcggc gcggttgaac tgtatcggaa
    16981 agggcaaaag taccgccttt tgcccttttt tcgttttcag acggcatttc ccgcttgaag
    17041 ccccgccgtt ttcagcaggc aggaccgaat gggaaatacc gggcggcgca ccgggtgccg
    17101 ctttgtgtat gggaacattg tgagaactta tttttattat gcttaaaatc atttccgcca
    17161 acgtcaacgg catccgctcc gcctacaaaa aaggttttta cgaatacatc gccgcatcgg
    17221 gcgcagacat tgtctgcgtg caggaactca aagcgcagga ggctgatttg tctgccgata
    17281 tgaaaaatcc gcacgggatg cacggtcatt ggcattgcgc cgagaagcgc ggttacagcg
    17341 gtgtggcggt gtacagcaaa cgcaaacccg acaatgtgca aatcggcatg ggcattgagg
    17401 aattcgaccg cgaagggcgg tttgtgcgtt gcgatttcgg caggttgggc gtcatttcgc
    17461 tttatttgcc cagcggcagc agcgcggaag aacgccagca ggtgaaatac cgtttcctcg
    17521 atgcgtttta ccctatgctc gaagcgatga aaaacgaagg gcgcgacatc gtcgtctgcg
    17581 gcgactggaa catcgcccac caaaacatcg acctgaaaaa ttggaaaggc aatcagaaaa
    17641 attcgggctt cctgcccgaa gagcgcgagt ggataggcaa agtcatccat acgctgggct
    17701 ggacggacat gtggcgcacg ctctatcccg atgtgccggg ttatacatgg tggagcaacc
    17761 gcgggcaggc gtatgcgaaa gatgtcgggt ggcgcatcga ttatcagatg gttacgcccg
    17821 aacttgccgc caaagccgtg tccgcacacg tttataaaga tgaaaaattc tcggatcacg
    17881 cccctttagt tgtggagtat gactatgctg ccgaataagg ttttgggtaa atatgattgg
    17941 aatgtggacg gaaaaacagg aataggcgcg gcatgggttg tcgctgcgtt tatcctgccc
    18001 atattggtgt gggcggtgtt tatgttgtcg cggatgcaag gctggcttgc gccgaccaag
    18061 gcaaacccga tatgggcgtt ggtgtggctg ctgatttgtc tgccctgcct gctgattgcg
    18121 gccaaatgtt tgggttggaa aggctggcgg cgggtggtga atatttttgt ctgcctgacc
    18181 gtctgcgcca tattgagcgt acccgcttcg ctgctcatcg cctttaccct ccgggacctg
    18241 ctcaaatagt cgcggacaat ggaaagtttc gagcttgagg atttgacgct gtggctgata
    18301 cgcgatgcgg atgaagcgga aatgtggata gaccgttggg cggtcagtta tcccgttgtg
    18361 cagatgtccg aagcgtccgc aaacggcagc acggaagcgt ggcaaagcgc gcttcagacg
    18421 gcattcgaac ggatacaggg caggcatatc gccgttgtcg cacacggcgc gggcgcggcc
    18481 gcattcttgg cgtggctgta ccgcgccgac atcctgacgc agaagaaact cgccggtatc
    18541 attctcgtat ctccgcgtcc cgatattttt cccgacgatg cggaacacac tttccaacgc
    18601 gtccgctgtc cttgccgtgc cgcattggtg gtgtccgaac acggcggcgt gccgcacggt
    18661 tgggcgcaaa aacaggcgga tttgtggaac gcccgcctgt tggtttcccc gcattcgggc
    18721 agtttgaacg gtatgctcgg cggctggcag tggggtatga agctgatgca ggaaatgttg
    18781 ctggcgtgaa cggtcgcttt atcggaattt cggttaaccg gggttgcaag gaaaataccg
    18841 tctgaaacag gcttcagacg gcatttccat tcaaacaggg cttacaggct gcccaacacg
    18901 atacgcaaca cgcggcgcat cggttcggct gcaccccaca aaagttggtc gccgacggtg
    18961 aacgcgctga tgtattcgcc gcccatgccc agtttgcgga tgcgtccgac agggacggac
    19021 agcgtgccgg taactttggc gggcgtcagc tcgcggatgc tggcttcttt ttcgttgggg
    19081 acgactttca cccagtcgtt cgcgcctgcc aaaatcgctt cgatttcgga aacaggcagg
    19141 tcttttttca acttcagagt gatggcttgg ctgtggcagc gcatcgcgcc gatgcggacg
    19201 cacaggccgt cgatgacggt cggattgccg ctgcgtccga ggattttgtt ggtttccacg
    19261 ccgcccttcc attcttcttt ggactggccg ttgcccaaat ccacgtcaat ccacggaatc
    19321 aggctgccgg cgagcggtac gccgaagttg gctttcggat agtcttcgct gcggaggaaa
    19381 tcggacactt tgcggtcgat atcgagaatc gcgctggaag gatcggcaag ctcgtccgcc
    19441 acttgggcgt gaatcgcgcc catgccgctg atgagttcgc gcatattttt cgcgcccgcg
    19501 ccggaagcgg cttggtaggt catgctggtc gcccattcga ccaaatcgtt ttggaacagg
    19561 ccgcccaaag ccatcagcat cagggagacg gtacagttgc cgccgatgta gtttttcacg
    19621 ccgtttttga gaccgttgtc gatgatgttg cggttgacgg ggtcgagggc gataatcgcg
    19681 tcgtctttca tgcgcagtga ggacgccgcg tcaatccagt agccgttcca gccgctgtcg
    19741 cgcagggctt ggaagacgga tttggtgtaa tcgccgcctt ggcaggtgac gataatgtcc
    19801 attttcgcca gctcggcaac gtcgttcgcg tccaataatg ttttagccgc ctgaccgaaa
    19861 tcaggtgccg cgccgccgac gttggaagag gtaaagaaaa acgcttcggg aatgtgggcg
    19921 aagtcgtttt cttctttcat acgctgcatc aaaaccgaac cgaccatacc gcgccagccg
    19981 acgaaaccta ctttcatgta ttgctccttg aggaaaatcg ttaataaaat tatgtatggc
    20041 gtaaagaaag cgttttaacg ccgtattggg aaaatgtaaa gttctttatc gaattttctt
    20101 tttgggattg gcgtttgcag cagtagggcg gtagaattcc atctgtttaa gaacaattat
    20161 caggaacgga tatggaagaa aaaaacgact acaccgacgc ggtatccgac aaccgcaacg
    20221 ggcaggaaat tgaagtcgga attgccggcg caggcgaccg gatacttgcc gcgctgctga
    20281 accaattgtt tacctttttg attttgttgg tgccgtttgt cggactgatt gccttcgccg
    20341 tcaaaaatga aggcaggatt ggcggccgtg aggaaatatt cgggctgctt ttgggcatga
    20401 cttccttttg ggtcggactg gcgggcattt tggcgtacac cgtcatccaa atctactata
    20461 tgagcaggga cgggcagtca ttgggtaaga aaatcatgag aatccgcgtg ttgaaaaccg
    20521 acggccgcaa tccgggtttt gtcggcacgg ttttggtacg cgaaatcgcg tggtcggttt
    20581 tggttgccat tattgccgcc gttatcggtc ttgcagtagg tgaaaacggg gaaaacgcca
    20641 tcaacctgct ggcattcctt gccaactttg tcctgctctt tatggtcaaa cgcgaccgcc
    20701 gcacgcttta cgacatattg gcggatacgg ttgtcgtcaa gctgcccaaa taaacggacg
    20761 cggcaaaatg ccgtctgaaa ggctttcaga cggcattttt atcttcagaa ccgctcaagc
    20821 atcgtgcagc gaagctgcgt gcaaagtgtt ttccattaat gtggcaatcg tcatcggacc
    20881 cacgccgccg ggaacgggcg taatcatcgc cgcccgttct tttgccgttt caaattccac
    20941 gtcgccgcac aggctgccgt cgtccaaacg gttgatgccc acatcgataa cgaccgcgcc
    21001 gggtttgatc cacccgcctt tgacaaagtt cggaatgccc acgccgacca ccaaaatatc
    21061 ggcggcggca acttcatcgg caagattttc ggtcgcactg tggcagaccg ttaccgttgc
    21121 gcgcgccagc agcaattcca acgcctgcgg gcggccgaca atattcgacg cgccgaccac
    21181 gaccgctttt ttccccttcg gatcaatgcc gtaagcctcc aaaagcgtca tcacgccctt
    21241 gggcgtacac gggcgcatca gcggcatttt gaccgccagc ctgccgacat tgtaaggatg
    21301 gaagccgtcc acgtccttat c
//
LOCUS       DAXFZR010000034        21309 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000034 DAXFZR010000000
VERSION     DAXFZR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21309
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.034"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            94..339
                     /locus_tag="ACFJAS_001068"
     CDS             94..339
                     /locus_tag="ACFJAS_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3982345.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEGLHHDTGHIGWRAMSKGK"
     gene            342..732
                     /locus_tag="ACFJAS_001069"
                     /pseudo
     CDS             342..732
                     /locus_tag="ACFJAS_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(772..2229)
                     /locus_tag="ACFJAS_001070"
     CDS             complement(772..2229)
                     /locus_tag="ACFJAS_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC3982346.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(2244..3074)
                     /locus_tag="ACFJAS_001071"
     CDS             complement(2244..3074)
                     /locus_tag="ACFJAS_001071"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC3982347.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(3151..3540)
                     /locus_tag="ACFJAS_001072"
     CDS             complement(3151..3540)
                     /locus_tag="ACFJAS_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC3982348.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(3687..4214)
                     /gene="nspA"
                     /locus_tag="ACFJAS_001073"
     CDS             complement(3687..4214)
                     /gene="nspA"
                     /locus_tag="ACFJAS_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC3982349.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNNVK
                     NVRSGELSAGVRVKF"
     gene            complement(4657..5832)
                     /gene="hemW"
                     /locus_tag="ACFJAS_001074"
     CDS             complement(4657..5832)
                     /gene="hemW"
                     /locus_tag="ACFJAS_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC3982350.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTVEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGLIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(5889..8360)
                     /gene="ligA"
                     /locus_tag="ACFJAS_001075"
     CDS             complement(5889..8360)
                     /gene="ligA"
                     /locus_tag="ACFJAS_001075"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC3982351.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     SNIPLRLHGENVPELIEVRGEVLMLKADFAGLNKRQAENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSVARQQGGLIAEEHIQELAYFQALGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGTVVKVNSLAQQHELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(8494..9780)
                     /locus_tag="ACFJAS_001076"
     CDS             complement(8494..9780)
                     /locus_tag="ACFJAS_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC3982352.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAVFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(9974..10546)
                     /gene="ampD"
                     /locus_tag="ACFJAS_001077"
     CDS             complement(9974..10546)
                     /gene="ampD"
                     /locus_tag="ACFJAS_001077"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC3982353.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            10630..11625
                     /gene="mltG"
                     /locus_tag="ACFJAS_001078"
     CDS             10630..11625
                     /gene="mltG"
                     /locus_tag="ACFJAS_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC3982354.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            11685..12305
                     /gene="tmk"
                     /locus_tag="ACFJAS_001079"
     CDS             11685..12305
                     /gene="tmk"
                     /locus_tag="ACFJAS_001079"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC3982355.1"
                     /translation="MKPQFITLDGIDGVGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            12527..13807
                     /locus_tag="ACFJAS_001080"
     CDS             12527..13807
                     /locus_tag="ACFJAS_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HFC3982356.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTGKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            14209..15240
                     /gene="lpxK"
                     /locus_tag="ACFJAS_001081"
     CDS             14209..15240
                     /gene="lpxK"
                     /locus_tag="ACFJAS_001081"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC3982357.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKGKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            15446..15979
                     /locus_tag="ACFJAS_001082"
     CDS             15446..15979
                     /locus_tag="ACFJAS_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC3982358.1"
                     /translation="MKLKTLLLPFAVLALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            15964..16071
                     /locus_tag="ACFJAS_001083"
                     /pseudo
     CDS             15964..16071
                     /locus_tag="ACFJAS_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            16201..16383
                     /locus_tag="ACFJAS_001084"
     CDS             16201..16383
                     /locus_tag="ACFJAS_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC3982359.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            16380..17141
                     /gene="kdsB"
                     /locus_tag="ACFJAS_001085"
     CDS             16380..17141
                     /gene="kdsB"
                     /locus_tag="ACFJAS_001085"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC3982360.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            17164..17679
                     /locus_tag="ACFJAS_001086"
                     /pseudo
     CDS             17164..17679
                     /locus_tag="ACFJAS_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            17836..17928
                     /locus_tag="ACFJAS_001087"
     tRNA            17836..17928
                     /locus_tag="ACFJAS_001087"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17870..17872,aa:Ser,seq:gct)
     gene            18201..18986
                     /gene="trpA"
                     /locus_tag="ACFJAS_001088"
     CDS             18201..18986
                     /gene="trpA"
                     /locus_tag="ACFJAS_001088"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC3982361.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIDSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVAAAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            19023..19895
                     /gene="accD"
                     /locus_tag="ACFJAS_001089"
     CDS             19023..19895
                     /gene="accD"
                     /locus_tag="ACFJAS_001089"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC3982362.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(19955..20566)
                     /locus_tag="ACFJAS_001090"
     CDS             complement(19955..20566)
                     /locus_tag="ACFJAS_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC3982363.1"
                     /translation="MRRAILLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            20674..20898
                     /locus_tag="ACFJAS_001091"
     CDS             20674..20898
                     /locus_tag="ACFJAS_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC3982364.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            20929..>21309
                     /locus_tag="ACFJAS_001092"
     CDS             20929..>21309
                     /locus_tag="ACFJAS_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982365.1"
                     /translation="MLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLFRSACVLTQGS
                     FEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYLSLTRDSCSVK
                     AERADSAALLEHFSGLVRQPPKNAN"
BASE COUNT         4636 a         5200 c         6474 g         4999 t
ORIGIN      
        1 cgatgatttt tattagctaa tttaagtaat ttaagcaagt tttttagata ctgaaactgg
       61 taattatgtt tatgtaagca taaggagttc agattgaagg attctacagg gcaaatgagg
      121 ctggcaacca aggatttggc ggaagccatt aaacgaggag aagtacgtag ttctgctttt
      181 acaacaaagc aactaaaggc aatcgaaaaa ggagaatcta aaataccgga ttacacttgg
      241 catcatcatc aagacactgg aaggatgcaa ttgattaatg aaggcttgca tcatgatacc
      301 ggccatatcg gttggagagc gatgagtaaa ggaaagtaac tatgtggaaa atcataaaag
      361 aggatagtga tgatttagga tttgcaatta aatgcttatt ctctcaatct attgatttaa
      421 atgaattcaa gttatggatt gaacaagtaa tacgcgatat gcccatcgag gacatccctt
      481 tttatatttt tgacttggcg gatttcaatg ggggaattgg agacattgac aatattgtag
      541 gttttgtttc gagttacagc ctatcaaaat cgaaaaaaaa tgccttgaca ggcattgcct
      601 tcttaagggg gatagatgtc tatagaccct cccgtttcaa aagaaaaagc attaaaagcc
      661 ttagagaaat atcctgaaat ttatcataaa tttaaatggt tctttccttt tgtagagctt
      721 ccgcttcttt aaaggacaat atgccgtctg aaaagttttc agacggcatt tttatttctt
      781 ccagtaggcg ggggtgaaga ggatgaagac ggtgaagatt tccagcctgc ccaagagcat
      841 tgcggtaacg cagatccatt tctgcatcac gtccaaaccg gcgtaattgc cggcgggccc
      901 gacttcgccc agtccggggc cggcgttggt aatgcaggcg atgacggcgg taaaggcggt
      961 ggtaaattcc ataccgctcg ccatcagcag gaagctgaag aggacgacgg tcataaagta
     1021 gatgaagatg aaggacataa cggtcagcgc gaggcggtcg ggtatggctt tgccgctgat
     1081 tttgacggtg cggacggctt tggggtgcag cagcaccatc atttcgcgca ggctgaattt
     1141 gaacaggacg agggcgcgta tggttttgat gccgccgccg gtcgagccgg agctggcgag
     1201 gatgttggcg aggaaaaaca tccacaggga aatcaggagc ggccattgtg cgaagtcggt
     1261 gttggacagc ccgtttgcca atccgatgga gacgaagttg aaggcggtgt agcgcaggga
     1321 ttcgccgaag ccggcgtaat ggccggtgtg ccacaggtac agggcggcgg caaggatgct
     1381 gccggagagc agcagcagca tcgtccggca ttcttcgtct ttccaatagg ttttgaggct
     1441 gcggctgttg agggcggcga aatggctggc aaaattgatg ccgccgaaaa tagtgaaaac
     1501 gatgatgact gcttcgatga ggggggagtt gtaataaccg atgctggcat cgtgggtgga
     1561 aaacccgccc agcgagaggg ttgccagcgc gtgacagacg gcatcgaacc aacccatccc
     1621 ggcaaaatgc aggcaggctg ccgcgaggat ggtgatcagg gtgtagccga accagagttt
     1681 tttcgccact tgggaaatgc gcggcgacat tttgctttct ttgtcaatgc cggggatttc
     1741 ggctttgaat aactgcgtgc cgcctacgcc gagcataggc aggatggcga cggcaaggac
     1801 gatgatgccc atcccgccca gccagttgag catatgccgc caaaagttga cggagggggc
     1861 gagcccgtcg acgtggggga tgacggtcgc gccggtggtg gtcagtcccg acatcgattc
     1921 aaaaaatgcg tcggtaaagc ccatattcgg gaaatacagg tacatcggca tcgcagccat
     1981 agcggcaaac gccagccaca acatcaggac gagggtgaag ccgtcgcgcg ggcgcagttc
     2041 gcgcctgaac cggagggtgg cgagccggac gatgcacgag ccggaaaggg taacggtcgc
     2101 ggtggtggcg aaggcggtgt acgcaccgtc cgaaaaggcg taggagaggg cggcgggtat
     2161 cagcaggata aaggaaaaca gcatacccag tcgggagagg acgtgggcga tgggcaggat
     2221 tttgtgcata gtggggcggt ccgttatttt gcgaagcttt tccagtctat gccgccggcg
     2281 gcttggactt gggtaatttc ttccgtttcg aggttgacgg cggtcagctg cccgccccac
     2341 agcgcgccgg tgtccagcga gatgacgttg tcggcattcg tgtagcccag cgaggaccag
     2401 tgtccgaaga tgatggtatg gtcgaggttt tgccgatcgg gggctttgaa ccacgggcgc
     2461 aggtaaggcg gcattttttt cactgtggat ttgtagtcga aatccagttc gtttttaaag
     2521 gtcagggcgc gcatccgcgt gaaggcgttg acgatgaagc gcaggcgggc atagccttcc
     2581 aaaccttcgt cccatgcggc cggtttgttg ccgtacattt tggagaagaa tttgacgtat
     2641 tttttgccgc gcagttcggc ttcggcttct ccggcgagcg attcggcttt ggctatgcgc
     2701 cattgcggca ggatgccggc gtgtatcatc acgcggccgc cctcgcgtat caaaagcggt
     2761 tgcgcgcgca gccagtcgag catttttccg ccgtcggggt gtttgagtat gggttcgatt
     2821 gtgtcgctgc gtttgagcgc gccttcgccg cagccgacgg cgagcaggta caggtcgtgg
     2881 ttgccgagga cgatttgcac gctgttttcg tgccggatgc agaattgcag cgtttcgagg
     2941 gatttcgggc cgcggttgac gatgtcgccc gtcagccaga gggtgtccgt gccgtggttg
     3001 aagccgattt tgccgagcag cgcggtcagt tcgtcgaaac agccttggat gtcgccgatt
     3061 gcgtaatgtg ccattgcggg tgttgtgaag tgggaaagtg ttgcggttcg gacggcacgg
     3121 ttggaaatct tatgccgtcc gaacgtggaa ttatgcgttc aaaacgagga cggcttccgc
     3181 ttcgacctgc acgcctttgg gcagcgaggc aacgccgacg gcggcgcggg cggggaacgg
     3241 ctcggtgata aattccgcca tcacttcgtt gaagacggca aaattgccca agtcggtcag
     3301 gtaggcgttg agtttgacga tgtcgtccag cgaaccgcct gccgcttcgg cgacggcctg
     3361 caggttttgg aacacttggc gcgcttcggt gtggaaatcg ccgttgccga ccacagtcat
     3421 cgtggcggga tcgagcggaa tctgaccgct catgtaaacg gtgccgccgg cgcgcacggc
     3481 ctggctgtat gcgccgatgg cggcgggggc tttgtcggta tggatgacgg ttttggacat
     3541 ttcggattcc tcaaaaaata gggcggcaga agccgcagcg ttcgggatta tcgtacaaaa
     3601 ccgccggctt gtgtagtttt aggcggtata tgtgccggaa acaaaaaacc gccgaaggct
     3661 cggcggtttg cggaataacg cgtatatcag aatttgacgc gcacgccggc ggacagttcg
     3721 ccggaacgga cgtttttgac attgttgact ttgccgatgt agttgtagcg gtagccggca
     3781 tccagatcga cattgggtgt aacggcatag cttacgcccg ccaatacgcc gaggccggcg
     3841 gaggttttgc tgaagctgtc gctgccgccc aagtgggcgg aagcgcggtt gaggctcaag
     3901 cgcgcgccga aatacggttt gacgggcgat tgggtgtcga agtcgtaaat gacggacgcg
     3961 ccgatgctgt aaagtttgaa atcggtggat ggggcttgtt tatagttttt gtagcgcgtg
     4021 taatcgacgg cgaagcggag gtcgttgatg cggtagcctg cggagatgcg cgggctgaag
     4081 cctttggcag aacctaaaga gcttgaggct ttggcgtgtg cggcatcggc ttggacgtaa
     4141 aagccggatg cgccttccgc cagtgcggcg gccgggagtg cgagggcaat cagtgcggca
     4201 agtgcttttt tcatattttg gttcctttat ggtcagggag aaaaattgtt aataatccgt
     4261 taaagaatcc tgccgtatta tactgaaatt ttctttttgc atcgtaatat tttcaatgcc
     4321 tcaagatacg cggcggcatc cggctgcttt gccgacggca aagccgttaa cccgcgcgtt
     4381 gcccttaaac ggcggcatca cgcggcggat gggtgaaact tgcaaacggt ttggaaaaaa
     4441 ccgcaaaggc ggggcggcgg catttgtcag attgttgcag gcgcaggcgt acggtttttt
     4501 gtgtgcggcg ttaccttagg cgtcggacat ttccggcggc ggctgtgccg tctgaaacgc
     4561 ccggcggggg atgcggctgc gtttcccatc gataggcata tttgccggcc gcgttcgggg
     4621 cgggttttac ccgggcggcc gccgatttgt ttgcgcttat aaaaaacact gcaacaaatc
     4681 gtttaaaaac aagcgtcctt tttcggtcgg gcggaatacg gtcgggtcgg tttccagcag
     4741 gcctttttgc cttgccgttt cgatttgcac catgattttg gcggcgggta cgcccgtgcg
     4801 ctcctgcaac atcgcggcgg gtacgccgtc ggtcaggcgc agggcgttca tcatgaattc
     4861 gaacggcaaa tcttcggcgg cgacggtttt gcgttcgacg gcttcgcccg gctgactttg
     4921 cattaaggcg aggtagtcgt tggggtggcg gcggcggacg gtgcgctcga tgcggtcggg
     4981 ataggagatt ttgccgtgcg cgcccgcgcc tatgcccaaa taatcgccga actgccagta
     5041 gttcaaattg tggcggcact gcatggtggg tttcgcaaaa gccgatgttt cgtagtggat
     5101 aagacccgcg ccttccagtg tgccgtgtac cgcgtcttcg atgtcgagcg ccgcttcgtc
     5161 ttgcggcaaa cctttcggcg gcgtatgacc gaacggcgtg ttcggctcca tcgtcagatg
     5221 atatgcgctg atgtgggttg cgtctgtggc gatggcggtt tgcacgtcgt ccaatgccgt
     5281 ctgaacggtt tggttcggca gggagtacat caagtcgata ttgactttat caaataattt
     5341 taaggcggta tcgatagcgg ttaaggcttc cttgccgttg tggacgcgcc ccagccttga
     5401 gagcatatcg tcgttgaagc tttgtacacc aatagaaagc cgcgtgatgc ctgcgtcttt
     5461 aaatccttga aacttctcga tttcaactgt acccggattg gcttccaacg taatttccgc
     5521 ttcgggctgc aagcgcaaca gcgaacgcac gccgcttaac aaacggtcaa tcgattccgc
     5581 ctgaaacagg ctgggcgtac cgccgccgaa aaatatcgtt tccaccggcc tgccccaaat
     5641 attgggcaat tcaagctgca agtcggtcag cagcgcgtcg atataggcgg cttcgggcaa
     5701 tccgtttttc aggctgtggg aattgaagtc gcaatacggg cattttttga tgcaccacgg
     5761 gatgtggata tagagcgaca ggggcggcag ggcggtgagt cgggtgcggt ttggaaagga
     5821 aatggtgtgc atggtgcggt tcggaaaagt ggacgatgcc gtctgaaggc ggttcggacg
     5881 gcatgggttc agcagagcag ggtaagcagt tcggcttcgc tgaggacgga aacgcccaag
     5941 gcattggctt tttccagctt gctgcccgcg gcttctccgg cgacgacgta gtcggttttt
     6001 ttggacacgc tgccggacac tttgccgcct gcggcttcga ttagggcttg ggcttggtcg
     6061 cgtttgaagg tgggcagggt gccggttaac acaaaggttt tgcctgccac ggctttattg
     6121 atgccgtctg aaccttgcgc cgcctcgtct tcagacggca tttgcgcgaa gaaggttttt
     6181 aggttttcga gcagggcggt gttttgcgct ttgctgcgcc acgcctgcca gtcggcaggg
     6241 agggctttgt cgttttgcag tccttctata ctttgtccgg cgagttccca taaggcttgg
     6301 gctttgtttt cgctgatttt gaaaccgggc aggcgggtaa tccagcgttg cggcccggca
     6361 tattgtgcgg caggcaggct gacggcttgg gcttgcgggg caacgcctgc ggcgagcagc
     6421 tcgtctatca tcgcctgctg ttcggcttgg gcgaagaagt gggcaatgga acgcgccact
     6481 accgtgccga tgtcgggcag gcaggcgagg acgggttcgg gggcgcggcg gacgcgttcc
     6541 aatgtgccga atgcctgcgc cagcgttttg gcggtgcgtt cgccgacgtg gcggatgccg
     6601 agcgcgaaca ggaagcgggc aagttcgggc gttttgccgg attctatgcc tgcgaggatg
     6661 ttttgcgccc acttggtcgg ttgttttttg ccgttttggg tattgtattt agacaggtcg
     6721 cctgaaaccg tttctgcgtc gccgttttcg ttttcagatg accctttatc cgccgtttcc
     6781 ttcatttttt gcagggtcgg aatatcgatg cggtagaggt cggcgaaatg gcggacgagg
     6841 tcttgggcga ccagctgttc gatttgtttt tcacccaagc cgtcgatgtc catcgctttg
     6901 cgcgaggcga agtggattaa gccttgcgcg cgttgtgcct gacaaagcat accgccgctg
     6961 catcgggcga cggcttcgcc ttcttcgcgt tcgatttcgc tgcggcagat ggggcagcgg
     7021 gccggcaggc ggtagggctt atggagcgga acggattcgg tttgcttggc ggacggtgtt
     7081 tcggcaaaca ggtcgtcctg ctgatgcccg atgccgtctg aaacggcaac ggcggtttcc
     7141 tgcatcgggc ggcgttcgaa aatcacgcgc acgacttcgg gaatcacgtc tccggcgcgg
     7201 cgcacgacga cggtatcgcc gacgcgcacg tctttgcgcg atacttcgtc ctgattgtgc
     7261 agggtggcgt tggtaacggt tacgccgccg acgaatacgg gttgcaggcg ggcaaccggc
     7321 gttaccgcgc ccgtcctgcc gatttgcacg tcaatcgctt cgacaatggt cagggcttct
     7381 tcggcaggga atttgtgggc aaccgcccag cgcggcgcgc gggagatgaa gccgagttcg
     7441 tgctgttgcg ccaagctgtt gactttgacg accgtgccgt cgatttcgta gggcagttcg
     7501 gggcgttttt gctgcatgtg ttcgtaaaac gccaatactt cgccgatatt tttgaaacag
     7561 ccgaaattgc cattgggcag actgaagccg agtgcttgga aataggcgag ttcttggatg
     7621 tgttcttccg caatcaaacc gccttgctgg cgggcgacgg agtaggggaa aaagtgcagt
     7681 ttgcgttgcg cggtgatgcg cgaatcgagt tggcgcaggc tgccggcggc ggcgttgcgc
     7741 ggattggcaa agggtttttg cccgttttcg gcttggcgtt tattgagccc ggcaaagtcg
     7801 gctttgagca tcagcacttc gccgcgtact tcgatgagtt cgggcacgtt ttcgccgtgc
     7861 agccgcaggg ggatgttgga tacggttttg acgtttcggg taacgtcttc gccggtcgta
     7921 ccgtcgccgc gcgttgccgc ctgcaccaat acgccgtcgc ggtagagcag gctgatggcg
     7981 aggccgtcga atttgggttc gataacgtat tcgggattgc cgccgtccaa gccgtcgcgc
     8041 acgcgttggt cgaaggcgta catttcggca tggtcgaaca cgccgttttc atcttgcggg
     8101 gaaaaggcgt tggtcagcga cagcatcggc acttcgtggc gcacttcggc aaagcctgcc
     8161 aaaggctcgc cgccgacgcg ctgggtcggg ctgtcgggca gcttgagctc gggatggttt
     8221 cgttccaacg cttcgagttc gcggaacagg cggtcgtatt cggcatcggg tatgctgggc
     8281 gcgtcgaggg tgtagtattc gtaggcgtag cggttgagga ggtcggtgag ttcgtgtatg
     8341 cgttgtgcgg tcgggttcat ggattcgtac aggttcagac ggcatggaca aatgccgtct
     8401 gaacggtaaa acgtgttgga aatggtcgga ttttacctga aaacaaaagg cggatgcacc
     8461 gttgccgatg tatccgcctt tgtgtttgcg gagttatgaa aacaggcgca gggcggtttt
     8521 gccgcccggt tcgataccga ctttgagcat ctcggactga cgcgccaata cataagtgcg
     8581 tacgtctttg agccattggg tcgaaacttc ttccattttg tcgttgacca gattcaggtt
     8641 caactgaccg gacaggcgta ccgccaaatc cataaacaaa tcgtcgaagg ttttttcgcc
     8701 tgccggagag tgcgggatgt cgagcagcat actgaagcct ttgtaggact ggttgtccaa
     8761 aagggcattg gtaaacggct cgttgttgag cgagcagatg gagaacatgg tcgagcccga
     8821 cgtgtcggta tagtggaacg cgccgtcgtc ttccaaaacg aaacccacgc ccgttacggc
     8881 ggaacgcagt tctacgccgc tgatgctggt cggcgaaacc aaatggatgg cgatggtctg
     8941 gtcgacgcgc gcgcagaatg cgtccagtgc ggaagccact tcgataaaga cggcaaggtc
     9001 ggtgtgcagc gtctgaccgc ccatgctttg tgcgaatgcg tccgcctggc ggttgaatgc
     9061 ggagagttct tcctgcgagg caagtccgtt gcggctgact gcctggatac ccacgataaa
     9121 tgcctgatag cggatgcccg ggatgggttc ggcaatctgg aaatggtcgt ccatggtgca
     9181 gccgacaatc tggtagcggc agcggttgga aaggcgcggc agtgcgtgca gttctttggc
     9241 ttcggtcagc gcgatatagg agatgaagtc gaagcgcacg tcaaaccagg gcagctcgac
     9301 cttcgacagc tctttgagcg taatcagcgg ttttgccggc gtttgcggaa cgggaacggg
     9361 ttttgccggc gcgtcggcag gtttcggtgc ggaatgtccg gtttggggtt cggagacggt
     9421 gtgggcggag ttgccgataa tgccgctttc ttccaaggcg gtttcgattt cggttttgaa
     9481 cggggaggct tttgcctgtt tctgcttggc gatgtagacg gcatcctgtt cttgcaggtt
     9541 gcgcatggcg gagtcttggg gtttggccgg ttttttgacc gccggttggg gtttcggcat
     9601 catgactggc ccgccggacg gtttgccgtc gcggacatgg ctggttttgc tgttgagcag
     9661 ggcatctttg tcggagtgtc cgaactggtc gcgcactttt ttgcggtatt ggttttcctg
     9721 atacatattg taggcgacaa cggcgaggac ggcggcgagg aacagtacga tgtaaatcat
     9781 ggcaatcact ttaaatttcg ggatgcagga tgcgcaaagt gcgggtactg cggttaaatc
     9841 gggcttgcgt ttccggcagt ctgacggaac ggccggttat aacgtttgaa ttataacgaa
     9901 aattgcaggg tctgacagca gtgtgtcgaa ataagcggaa attttccgaa atgccgtctg
     9961 aaatctgtgg ttttcagacg gcatttctgt ccacgggaaa ccctttttcc cgtatccgcc
    10021 gccagtcgaa aaaatggccg gggtcggttt tgcggccggg tgcgatgtct tggtgtcccg
    10081 ttaccgccgt gatggggtag cggcggcaga gggctgccaa cagcgtttcg agcgaacggt
    10141 attgcgcttc ggcaaacggt tcgaaatcgc agccttccag ttcgatgccg atggaaaacg
    10201 cgttgcactt ctcccgcccc cgaaacgagg atgcgccggc gtggtacgcc atgtcgccgc
    10261 atgatacgaa ctgcaccgtt tcgccgtcgc gtgcaatcag gaaatggctg gacacgtgca
    10321 aagtgtgtat caggctgaag aacggatgtc cgtcggggtc gagccggttg gcaaacagct
    10381 tttccaccgc atccgtgccg tattcgaacg gcggcagcga aatgttgtgc agcacgatca
    10441 gggaaaccgt ttctcccgtt tccctcgggc tgaaattggg cgacggggta tggcgtatgc
    10501 tttgaagcca gccgttttgc cagtgtgctc cggcgtgatt gtccatgatg ttcttcctgt
    10561 ccggcgggca atttgggtta tactgtcgcc cgaattttaa gacgtattcc gaatgctggg
    10621 aatcctacca tgttgaaaaa aatgttgaaa tggactgccg tttttttgac cgtatcggca
    10681 gccgttttcg ccgcgctgct ttttgtccct aaagacaacg gcagggcata ccgaatcaag
    10741 attgccaaaa atcagggtat ttcgtcggtc ggcaggaaac ttgccgaaga ccgcatcgtg
    10801 ttcagcaggc atgttttgac ggcggcggcc tacgttttgg gtgtgcacaa caggctgcat
    10861 acggggacgt acagattgcc ttcggaagtg tctgcttggg atatcttgca gaaaatgcgc
    10921 ggcggcaggc cggattcggt taccgtgcag attatcgaag gttcgcgttt ttcgcatatg
    10981 aggaaagtca tcgacgcaac gcccgacatc ggacacgaca ccaaaggctg gagcaatgaa
    11041 aaactgatgg cggaagttgc gcccgatgcc ttcagcggca atcctgaagg gcagtttttt
    11101 cccgacagct acgaaatcga tgcgggcggc agcgatttgc agatttacca aaccgcctac
    11161 aaggcgatgc aacgccgcct gaacgaggca tgggcaggca ggcaggacgg gctgccttat
    11221 aaaaaccctt atgaaatgct gattatggcg agcctgatcg aaaaggaaac ggggcatgag
    11281 gccgaccgcg accatgtcgc ttccgtcttc gtcaaccgcc tgaaaatcgg tatgcgcctg
    11341 caaaccgacc cgtccgtgat ttacggcatg ggtgcggcat acaagggcaa aatccgtaaa
    11401 gccgacctgc gccgcgacac gccgtacaac acctatacgc gcggcggcct gccgccaacc
    11461 ccgattgcgc tgcccggcaa ggcggcactc gatgccgccg cccacccgtc cggcgaaaaa
    11521 tacctgtatt tcgtgtccaa aatggacggc acgggcttga gccagttcag ccatgatttg
    11581 accgaacaca acgccgccgt ccgcaaatat attttgaaaa aataaaccat gccgtctgaa
    11641 aagtttgtgt tttcagacgg cataccctta ccggaactgc aagcatgaaa ccgcaattca
    11701 tcactttgga cggcatagac ggtgtcggaa aatccaccaa ccttgccgtc atcaaggcat
    11761 ggtttgaacg gagggggctg cccgtgctgt tcacgcgcga gccgggcgga acgccggtcg
    11821 gtgaggcctt gcgcgaaatc ctgctcaacc ctgaaaccaa agccggtttg cgtgcggaaa
    11881 cactgatgat gttcgccgcg cgtatgcagc acatcgagga agtcatcctg cccgcgcttt
    11941 cagacggcat ccatgtcgtg tccgaccgtt ttaccgatgc gaccttcgcc tatcagggcg
    12001 gcgggcgggg gatgccgtct gaagacattg aaattttgga acattgggtg cagggcggtt
    12061 tgcgccccga tttgaccctg ctgttggatg tgccgctgga agtatcgatg gcgcgtatcg
    12121 gacaggcgcg cgagaaagac cggttcgagc aggagcaggc ggatttcttt atgcgtgtgc
    12181 gcggcgttta tctcgaccga gccgccgcct gtcccgaacg gtacgccgtt atcgacagta
    12241 accgcagctt ggatgaagtc agaaacagca tagaaaaagt gttggacgga catttcggct
    12301 gttgatgcgg caaatattga aacaagcgca tccgcccgcg ccgaaaacca aacggcagtg
    12361 ccgcaggtga aaatggcggt atgcgccaaa ctttcggcat gatagaatta cgctcggtta
    12421 caaggcagga tgcgtcggca atattaacga accgcccgta acatgatgac ccgaaagcgt
    12481 ttcggacagt tcgattcaaa tctttttctc gcaacaggat tgacacatgg aaaactcatt
    12541 gaaagaagcc gccctcaagt tccacgaatt acccgttccg ggcaaaattt ccgttacccc
    12601 gaccaaatct ctggcgaccg acaaagattt ggcgttggcg tactctccgg gcgtagccgc
    12661 cccctgtatg gaaatccatg ccgatccgca aaatgcctac aaatacaccg gcaaaggcaa
    12721 cttggtcgcc gtcatttcca acggtacggc cgttttgggc ttgggcgaca tcggcgcgct
    12781 ggcgggcaaa cccgtgatgg aaggcaaagg cgtattgttc aaaaaattcg ccggtgtgga
    12841 cgtgttcgac atcgaaatcg acgaaaaaga cccgcaaaaa ctggtggaca tcatcgccgc
    12901 tttggagccg actttcggcg gcatcaacct cgaagacatc aaagcacccg agtgttttta
    12961 catcgaacgc gaattacgca aacgctgcaa aatccccgta tttcatgatg accagcacgg
    13021 cacggccatc attaccgccg ccgccgtatt gaacgccctg cgttataccg gccgtaaaat
    13081 cgaagaagcg actttggtgt gctccggcgc aggtgccgcc gcgattgcct gcctgaacca
    13141 actgctggat ttgggcttga aacgtgaaaa cgtaaccgtt tgcgactcca aaggcgtgat
    13201 ttaccaaacc cgcgaagaca aagaccgcat ggacgaatcc aaaaaattct acgccgttga
    13261 agacaacggc cggcgcgtac ttgccgatgc ggttaaaggc aaagacatct tcttgggcct
    13321 ctccggcgca aacctgctga cgcctgaaat gttgaacacg atgaacgaaa aacccatcgt
    13381 gttcgccatg gccaacccga atccggaaat cctgccgccg ctggcgaaag aaacccgtcc
    13441 ggacgtggtt atcggtaccg gccgctccga cttcccgaac caagtgaaca acgtattgtg
    13501 cttcccgttc atcttccggg gcgcgttgga tgtcggcgca accaccatca acgaagaaat
    13561 gaaacgcgcc tgcgtgtatg ctttggcaga tttggcgatg gaagaagtaa ccgaagaagt
    13621 ggttgccgct tacggtaaga aattcgaatt cggcgcggaa tacctgattc ctactccgtt
    13681 cgattcccgc ctgctgcctc gcgtcgctac ggctgccgcc aaagcagcga tggaaagcgg
    13741 cgtggcaacc cgtccgattg cagatttgga agcttacgct gccaagttgg gcgaatggaa
    13801 gctgtaagcc gtttgtggtt aaaatgccgt ctgaactgtt ttcaggcggc attttgttgt
    13861 cagattgata aatgaaagat actggaaaat gaaagagatg aaacctgtcc gttatcatat
    13921 tggcgatata cccgaaactt caaaacaaac cgcccccggt catgacgaca gggcagtggg
    13981 tgttgacgat gattgttttc atgattcctt tggtcaatat cgttatgttt tttgtttggg
    14041 cgttcggcag aggcaacccc aaccgtgcca atttctgtaa agcactgttc ttatttacct
    14101 tgttggttcg cttatcggtt tgattttcat attgcttata ggtgggtctg catcgggtac
    14161 gcattattaa tgtgccggct gattctgctt cgaagatttg tatcgaatat gccaaatttt
    14221 tttaaatttc ataccgttat cgaacggcat tggcaaaaac cttatccggt tttgtctttt
    14281 ctgctcaagc cgctttccgg gctgtttgcc aaaattgcgg caaaatggcg ggcggatttt
    14341 ttatcgggaa aacggcaaag cgaaaagctg tccgtgcctg tggtcgtggt cggcaacatc
    14401 cacgcgggcg ggacgggcaa aacgccgatt gccgccgcgc tggtgtcggg cttgcaggaa
    14461 aaaggtgtca aggtcggcat catcagccga ggctacgggc gcaagggcaa ggcggtttat
    14521 gtattgaatg ccgcgagccg tgcggaagat gcgggcgacg agcctttgct gctgttccgt
    14581 aaaacaggtg cgccgacggc agtgggcagc agccgtgtgg aggcaggcag ggcgttgctg
    14641 gcagcgcatc cggagcttga attgattgtg gcggacgacg gtttgcagca ttacgccctg
    14701 caacgcgatg tggaaatcgc ggtgtttccg gcggcggata cggggcgtac ggatttggac
    14761 ttgctgccca acggcaattt gcgcgaacct ttgtcgagat tggaaagcgt ggatgcggtg
    14821 gtggtcggcg gcagggcggc ggatggtttt atgccgtctg aacatttgtt cggcagccgt
    14881 attgaggcgg gtgcggttta ccgtttgaac cgtccgtctg aaaaactgga tatttcgaca
    14941 ttgtcaggga agcgcgtcgc agcggttgcc ggtatcgcca ggccgcagcg tttcttcgat
    15001 actctgacac acatgggcat ccgccttgac caaacggttg ctctgcccga ccatgccgat
    15061 attttcaatc gggatttgcc gcctgccgat gtggtgctgg taactgaaaa agatgcggtc
    15121 aaattttcag acggcatttg caccgataat gtttgggtgc tgccggtttg tgcgataatc
    15181 gaacctgatt tggcggagtt tgtgctggag cggttggagg gtgtaccgaa ggccgtctga
    15241 aagcgcggtt tggacgaagt gataacggac tggaataaga acgccctacg ccgtcattcc
    15301 cgcgcaggcg ggaatctaag tctcgaattt tcaggaatgc ctaggagact tcagaaatcc
    15361 caaatctccg gatttccact tgggcaggaa tgagaaagcc ggttgtattt tttatctgca
    15421 ttaatcattc gttaaaggag tggatatgaa gctgaaaacc ttgttattgc ccttcgccgt
    15481 actggcattg tgtgccaacg catttgccgc cccgcccggc gacgcgtcgt tggcacgttg
    15541 gctggatacg cagaatttcg accgggatat agaaaaaaat atgattgaag gctttaatgc
    15601 cggatttaaa ccgtatgcgg acaaagccct tgccgaaatg ccggaagcga aaaaagatca
    15661 ggcggcagaa gcctttaatc gttatcgtga gaatgttttg aaagatttga ttacgcccga
    15721 agtgaaacag gctgtccgca ataccttatt gaagaatgcc cgtgaaatat acacgcaaga
    15781 agaaattgac ggcatgattg ccttttacgg ttcgcctgtc ggtcagtccg tcgttgccaa
    15841 aaatccgcgc ttaatcaaga aatcgatgag tgaaatagcg gtatcttgga ctgcattgtc
    15901 agggaaaatc gcgcgacatc atctgcccga gtttacggaa gagttacggc gcatcatctg
    15961 cggcggtata gtggattaaa tttaaatcag gacaaggcgg cgaagccgca gaccgtacaa
    16021 atagtacggc aaggcgaggc aacgccgtac cggtttaaat ttaatccact ataaaaatcc
    16081 cgatgcgggc tgcaagcagg ccggacagct tgggaaaagg tatcagaagt aaataatagc
    16141 cgcctgaaat attgcggagg gcatccgatt gattaaacca tcaaacccga aagcaacctt
    16201 atggaaaaaa aattcttaga catcctcgtc tgccccgtta ccaaaggcag gctggaatat
    16261 catcaggaca aacaggaatt gtggagccgt caggcgaagc ttgcctatcc gattaaagac
    16321 ggcattccct atatgctgga aaacgaagca cgagcgttga gcgaagagga actcaaagca
    16381 tgaccgaatt cgtcgtattg attccggcgc ggctggattc gtcgcgcctg cccggaaaag
    16441 ccttggcgga cattcacggc aaaccgatgg tcgtgcgcgt tgccgaacag gcggcaaaaa
    16501 gcaaagccgc gcgcgtcgtc gtcgccaccg atcatcccga cattcagacg gcctgtcagg
    16561 cgcacggtat cgaagtcgtc atgacttcaa accggcacga aagcggcacg acgcgccttg
    16621 ccgaagccgc cgccgcgctg aagctgccgc cgcatttgat tgttgtgaac gtacagggcg
    16681 acgagccgct gattgccccc gaactcatcg accgcaccgc cgaagtactc gtcgaaaaca
    16741 acgtccaaat ggcgaccgcc ggccacgaat tgcacgattt cgacgaattg atgaatccca
    16801 atgccgtcaa agtcgtcctc gacaaaaacg gcaacgccat ctacttcagc cgtgccccga
    16861 ttccctatcc gcgcgatgcg atgcgtgccg gaaaacgcga aatgccgtct gaaaccgccg
    16921 tcctacgcca tatcggcatt tacgcctacc gcgccggctt cctgcaacgc tatgccgaaa
    16981 tgagcgtctc gccgctggaa accatcgaat cgctggaaca actgcgcgtc ctgtggcacg
    17041 gctaccccat cgccgtcgaa accgccaaag aagcccccgc cgccggtgtg gatacgcagg
    17101 aagatttgga cagggttcgc gctgtatttc agaccgtata aaacaggttc aaagggaaaa
    17161 gatatgcagc aacatattga aaagtggcaa cacttgagcc gtgaagaaca gaaaatcctt
    17221 gccgaagtat ggggtctcgt gcaaaacgat gatcaggagg tttactatga aatgctcaaa
    17281 ttgaacgcac ctgatgaagt cagcggtgaa ttttggttca gaatggcaga aacactcagc
    17341 accctgccgc ccaatcgttc cctcgacctt agaatgaacg gcggcaggct gtcgaccgcc
    17401 gtatccatcc tttccgccat gattgaagac aatcccgaca taccgcagct ttgggcgtaa
    17461 aaaattaccg ccctcaatta tttggtacac ggacacaaag cccgtgccga cggtttggta
    17521 caacagcccg acaaagcggc agaagccaac gaagaggaat acctgaccaa agccctgtcg
    17581 caaaacctgc tgtcaacatt ggatgccgca cttgcacgtt ttcctgaaga cgcgtggttt
    17641 caggaaatca aacaggatgc acaaaagcat tttgcttgag gacgtggcgg tcaggaatat
    17701 ttccattcag gaagaaaaga agtgcctaat tgggtataat cagggtaaat cttattttat
    17761 ttcagaagat taatatttgt tttccgtttt tccttgacgg tatcggaaaa gttgattata
    17821 gttacagctt ccttaggagt aatggctgag aggctgaagg cacttccctg ctaaggaagc
    17881 atgtgggatc aacctgcatc gagggttcga atccctctta ctccgccaga taaaaaatag
    17941 acgctgtgtt ttacagcgtc tattttttta tgcaatttta tagcgggttg gcgcaaaccc
    18001 ggtatggtat tgccctgtct tgattctgaa ttttgttaat ccgagatgtt tgccgtttat
    18061 ttttgcctcg ttcaaacggc ggctctgatc tgcgcggttt ctgtttgccg tattcgccta
    18121 tccgtaccgc aaatgttata ctgggaaaaa ttcaccgatt gtattttacg gcgtatttgc
    18181 cgataggatg gaagagacaa atgagcagaa tccggcaggc ttttgccgct ttggatggcg
    18241 gaaaggcatt gattccctat attgcggtgg gcgaccccga tattcggaca actttggcat
    18301 tgatgcacgg catggttgca agcggtgcgg atattttgga gttgggcgtg ccgttttccg
    18361 atccgatggc ggacgggccg gttattcagc gtgcggcgga gcgggcgttg gcaaacggga
    18421 tttcgctgcg cgatgtcttg gatgtcgtca gaaaattccg tgaaaccgac acgcaaacgc
    18481 cggttgtttt gatgggatat ttgaatccca tacataagat gggttatcgg gagtttgctc
    18541 aggaagccgc aaaggcgggg gtggacggcg tgttgacggt ggattccccc atcgaaacca
    18601 tcgattcttt atatcgcgag ctgaaggata acgaggtcga ctgtattttc ctgattgcgc
    18661 cgacgacgac ggaagaccgt attaaaacca ttgccgagct ggcaggcgga tttgtctatt
    18721 atgtttcgct caagggcgta acgggcgcgg caagtttgga tacggatgag gtttcgcgta
    18781 aaatagagta tttgcgtcag tatatcgata ttcccatcgg tgtcggtttc ggcatcagca
    18841 atgcggaaag cgcgcgcaaa atcggccggg ttgccgccgc gatcattgtc ggcagccgga
    18901 ttgtgaaaga gattgaaaac aatgcgggca acgaggctgc cgccgtcggt gctttggtca
    18961 aagagttaaa ggatgccgtg cgctgattct gtcgcgcatt ctaaatgttt taggagttgt
    19021 ccatgagctg gttagataaa atcctgccac ccaaaatcaa gaatcgtggg aaagacggtt
    19081 cttccaatgt tcccgagggt ttgtggcgca aatgcccgtc ttgttcggca accgtttatt
    19141 cgaccgagtt gcagcagaac aatcaggtct gcccgaaatg caaccaccac aatccgttat
    19201 cggcgcgcca gcgtttgaac ctgcttttgg atgaggaagg cagggaggaa gttgcgggta
    19261 atgtcaaacc gaccgatcct ttgaagttta aagacagcaa aaaatatccg gaccgtttga
    19321 gtgcggcacg caagctgacc ggggaagatg acgcgctggt ggtgatgaag gggatgatga
    19381 acggtctgcc cgtcgtcgtt gccgcgtttg aattccgctt tatcggcggt tcgatgggtt
    19441 cggttgtggg cgagcgtttc gtacagggtg tccgccgtgc ggttgccgat aattgtccgt
    19501 ttgtctgtgt ggcggcttcc ggcggcgcgc gtatgcagga gggtgtaaac tcgctgatgc
    19561 agatgacgaa aaccagtgcc gcgctgcatt tgctgacgga aaaacgcctg ccgtttatat
    19621 cggtgttgac cgatccgact atgggcggcg tatctgccag cttcgcattt ttaggcgatg
    19681 tcgtgcttgc cgaaccgaac gcgctgatcg gttttgccgg tccgcgcgtg attgagcaga
    19741 cggtgcgcga aacgctgccg gaaggcttcc aacgcgccga gtttctgctg gaaaaaggcg
    19801 cgatcgacca gattgtcgac cgccgcgata tgaagcggcg catcagtgat ttgattacgc
    19861 tgttgtgccg tcaggacaaa gtttccgcca cctgatggct gatgaatcga atgccgtctg
    19921 aaaccgatgt ttcagacggc atttttgtgt ctggttattt gtcgtgcggc ttcatcgacg
    19981 gggcatagcg tccggcacgt tctttcaggc gttgtaccaa accttgcgtg tcggcgggta
    20041 cgccgccctc gcagaatgcc tgatacagga cggcgcgcaa tgcgtcgttg cgcccaagtg
    20101 tgccgcctat cggtttccat tcggcgtttc ggggctgtat ccagcggctg ttgaccgtat
    20161 cgccgtatcc gaatactttg taggaggaaa gtttgccgtt gccgaacccg atggaggagc
    20221 gggcgcagaa tatgccttcg gcagtcaggt tgtcgtagcc tttgtcggag cggatattga
    20281 gaatgtagcg gatgctgccg tcgggcgcgg gcataatttg caggctgtcg agcaggattt
    20341 tcggctgttt gccgtaattt tcatccacat aaatgtcaaa ccagccgtcc gagtgcgcat
    20401 cgggcagagg cggcagttcg gcggtatgtt ctttaaattc gcgggcggcg gcttcttcgg
    20461 gcgtttcgcg gtagcgggtg ttgatcggcg tgtctttttg gctgaagccg gcggcaaggg
    20521 acgtgccgac agtcagggtc agaatcagaa ggatggcgcg gcgcataagt ttctccaaat
    20581 tgaaaacggc tttattttat ggtttggcgg gaagggctgc aagcaatcgg ggtataatct
    20641 gacctgattt tcattttaaa gcggtgtcga accatgaaca gcgaaacttt agacgtaacc
    20701 ggattgaaat gtcccctgcc gattttgcgg gcaaaaaagg ctttggcgca aatgcggcag
    20761 ggcgaggtat tgaccgttct ggcgaccgac ggcggcgcgc cgggggactt tgaggctttt
    20821 tgccgccaaa ccggtcatgt gctgttggat tcttccgaac aggacggcgt gttcacactg
    20881 gtcgtcaagc acaaataaat gccgtctgaa atgcggatgt cctgattgat gttattgctg
    20941 tcggcatttg tgcttgctgc gtgtaatgcc caaaaccggc aatccggtgt acggcatcag
    21001 gattttgcac gcgtagccgt cgatttgttt cggagtgcct gcgtcctgac tcaaggtagt
    21061 ttcgaaccgg tttccaagtt tgccgctgtc ggacattttg tccctgtcgg aaaggaagac
    21121 ctgtcccgtc tgccgcccgc tgttgcagaa cccgatgtac aggcgttgtg gacgcttgaa
    21181 cgcgatggcg gcatttatta tttgagcctg acgcgcgaca gttgcagtgt gaaagccgag
    21241 cgggcagaca gtgccgcgct tttggaacat ttctcagggc ttgtccgaca gccgccgaaa
    21301 aatgccaat
//
LOCUS       DAXFZR010000035        21174 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000035 DAXFZR010000000
VERSION     DAXFZR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21174
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.035"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..413
                     /locus_tag="ACFJAS_001093"
                     /pseudo
     CDS             1..413
                     /locus_tag="ACFJAS_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
     gene            601..1056
                     /locus_tag="ACFJAS_001094"
     CDS             601..1056
                     /locus_tag="ACFJAS_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982366.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            1125..1502
                     /locus_tag="ACFJAS_001095"
     CDS             1125..1502
                     /locus_tag="ACFJAS_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC3982367.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFTIKSSPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(1566..3779)
                     /locus_tag="ACFJAS_001096"
     CDS             complement(1566..3779)
                     /locus_tag="ACFJAS_001096"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC3982368.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATSEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            3922..4563
                     /locus_tag="ACFJAS_001097"
     CDS             3922..4563
                     /locus_tag="ACFJAS_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC3982369.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            complement(4635..5273)
                     /locus_tag="ACFJAS_001098"
     CDS             complement(4635..5273)
                     /locus_tag="ACFJAS_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC3982370.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            5529..6380
                     /locus_tag="ACFJAS_001099"
     CDS             5529..6380
                     /locus_tag="ACFJAS_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC3982371.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            6446..7108
                     /gene="exbB"
                     /locus_tag="ACFJAS_001100"
     CDS             6446..7108
                     /gene="exbB"
                     /locus_tag="ACFJAS_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC3982372.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            7111..7545
                     /locus_tag="ACFJAS_001101"
     CDS             7111..7545
                     /locus_tag="ACFJAS_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC3982373.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQANKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(8046..8474)
                     /locus_tag="ACFJAS_001102"
     CDS             complement(8046..8474)
                     /locus_tag="ACFJAS_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC3982374.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(8539..10287)
                     /locus_tag="ACFJAS_001103"
     CDS             complement(8539..10287)
                     /locus_tag="ACFJAS_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC3982375.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALNGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            10613..12046
                     /gene="ccoN"
                     /locus_tag="ACFJAS_001104"
     CDS             10613..12046
                     /gene="ccoN"
                     /locus_tag="ACFJAS_001104"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC3982376.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            12073..12684
                     /gene="ccoO"
                     /locus_tag="ACFJAS_001105"
     CDS             12073..12684
                     /gene="ccoO"
                     /locus_tag="ACFJAS_001105"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC3982377.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            12689..12859
                     /locus_tag="ACFJAS_001106"
     CDS             12689..12859
                     /locus_tag="ACFJAS_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC3982378.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            12883..14202
                     /gene="ccoP"
                     /locus_tag="ACFJAS_001107"
     CDS             12883..14202
                     /gene="ccoP"
                     /locus_tag="ACFJAS_001107"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HFC3982379.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            14546..15955
                     /locus_tag="ACFJAS_001108"
     CDS             14546..15955
                     /locus_tag="ACFJAS_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC3982380.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            16056..19262
                     /gene="recC"
                     /locus_tag="ACFJAS_001109"
     CDS             16056..19262
                     /gene="recC"
                     /locus_tag="ACFJAS_001109"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC3982381.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSETVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAADRMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELASSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QLAWSEPHIGEAWEPAEPFEPQHAEQIAETYIGARREGQDFSQTAARIAAESLLPSGE
                     LGKLWQQSFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILTGSLSNLYQCGQVFYAYG
                     KPNAPQRVAFLLEHLIFCAVMPSEAETRQTFIVQFGETEVLAEIAQDRALQLLSEWMA
                     FFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKARTAYYGSKVSKGQKDYTEVAL
                     VFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(19332..20900)
                     /gene="mtrF"
                     /locus_tag="ACFJAS_001110"
     CDS             complement(19332..20900)
                     /gene="mtrF"
                     /locus_tag="ACFJAS_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC3982382.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AHFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVTSTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPVP"
BASE COUNT         5236 a         5047 c         5794 g         5097 t
ORIGIN      
        1 cggcagacgg ctacaaagcc attgcccata ttcaagccgg cgaccgcgtc ttcgccaaag
       61 atgaaaccag tggaaaaact ggatacaaac ctgttaccgc ccgatacggc aatccgtacc
      121 aggaaaccgt ttacattgaa atttcagacg gcatcggtaa cagccaaacc ctgatttcca
      181 accgcatcca tccgttttat tcgggcggca aatggattaa agcggaagat ttaaaggcgg
      241 gaagcagact gttatccgaa agcggcagaa cccaaaccgt ccgcaacacc gttgtcaaac
      301 caaaaccgct caaagcctac aatctgaccg tcgccgattg gcatacctac ttcgtcaagg
      361 gcagtcaggc ggaaacggaa ggggtttggg ttcataattc ttgtccgcct aaaagaacag
      421 gaagctccaa gaatgaaaaa catggagacg gcggtcgaag tcaaatatca gcggaatcaa
      481 gaattgctga attaaaaaat aaaattattc ccggaatgtc caaaaatgaa cgcttaaaga
      541 ttaagcaaaa aatcaaaaat attgccaaaa atgccaatcg aaaaacaaaa ggagaagagc
      601 atggtcgacg cggtcgttaa aactcccgaa tttttacctt ttacttctgt aggaatattc
      661 cgttttggtg ctgatataac acagtacaaa aacatactgg aaacttttat gtatgaacct
      721 cctgatgagt ttggaacaga atattatgaa tctcccgact caaatttact catttccgtc
      781 aaaaaaaaca aaattatttc gatattttgt tatcaggagc tatattttat gggcgttaat
      841 ataataggtc tgaattttga agattttaaa cagcttttcc atcaccctaa atcatacggt
      901 gttgataaat attatttatc aaatgaatct tatccgacat atgtctatga gtttgatgaa
      961 ataggagtac aagcatggga agcaaaaggg aaaattgtta ctatcattgc cgggggcaaa
     1021 gacaattatt cgacagaacc ttactacgat gaataacatt acagaaaaga aacaagaaac
     1081 ggtacatttg ataaaaacct aaatcgtatt ggagattaat tattatgtgc gagttcaagg
     1141 attttagaag aaacatccct tgttttgaag agtatgacga aaattcattt attggtaaat
     1201 ggtatgatga cggggtgtgg gatgatgaag aatattggaa gctggagaat gatttaatcg
     1261 aggttaggag aaaatatcct tatccgatgg atataccaag ggatatcgtg attggaatcg
     1321 gtaccattat tgagttttta atggttccaa attggaaact ttttacaatt aaatcttccc
     1381 cttggttgcc tgatagtgtg ggaattcatg aacgttatga aagattcaca acgatgctcc
     1441 gttatatttt taccgagaaa gacatagtca acgtgcgatt tgattattac aacaaaaaat
     1501 agcaaaaccc ggcaatcaaa atgccgtctg aagcgttatc cggctttcag acggcatttt
     1561 tgtatttaaa gccgggtaac gctcaatacg cctttgacat cgccgaggcc ggcgaggacg
     1621 cgcgggaggt cgttgacttg tttgacttcg agcgtgaacc tcatgctggc ttccaagtcg
     1681 cgggactggg tttgtacggc ggtaacgttg agtttgtggc gggcgagcgc gtcggatacg
     1741 tcgcgcaaaa gcccggagcg gtcttgggcg cggatttcga tatcgacggc gaacacttgc
     1801 ccttcctgca acgccgccca acttgcgtcc agtacttttt cgggcgcgtg ttcggcaagg
     1861 tgtcggaaag aggggcaggt tttgcggtgg acggaaatgc cgcgctcgcg ggtaacgaag
     1921 ccggcaatat cgtcgggcgg cgcgggtttg cagcatttgg caagcgtggt catcaagccg
     1981 tcttcgccgt cgatgagcac gccggttttg ccaccttttt tgattttgga ctgtttgacg
     2041 atggtggttg cgctgacggg cacgggcggc ggttcgttca gcgtgccgca ggctttttgg
     2101 atggcgcggt tggaaatttc gccttgtccg acggcggtgt agaggtcttc tggctttttg
     2161 tagccgagat tttcggcaag ctcttgcagg ttgggtttgg gcgtgagttt ggcaagctgc
     2221 ttgtcgagtt ggacacggcc ttcttcgcgc acggtgtcgg cgttttgctg gcggatgtag
     2281 gcgcggattt tgccgatggc cttgccggat ttgacccagc cttcgtaaag ccagttgacg
     2341 gaaggatgcc cttctttggc ggtaatgatt tcgacgcgct gtccgttttc gagcggggtg
     2401 gacagcggca caatctgccc ttcgactttc gcgccccggc agcggtcgcc gatgctgctg
     2461 tgcagggcgt aggcgaagtc gatgggggtt gcgcccgttg gcagagagag gactttgccg
     2521 tgcggggtca aaacataaat cgtgtcgttg aaaagctcgg ttttgaaggc ggcggcgagg
     2581 tcttccttgc cgctttccgc catattttcg cgccagtcca agagttggcg caaccaggcg
     2641 attttttgtt cgtaggcgga atcgcctttg ccgccttctt tgtaacgcca gtgggcggcg
     2701 acaccgaatt cgttgaattg gtgcatatcg aaggtgcgga tttgcacttc cacacctttg
     2761 tcttccgggc cgacgatgac ggtgtgcaaa cttttataac cgttgccttt ggggttggcg
     2821 atgtagtcgt cgaactcgcc gggaatgggc tgccagaggc tgtggacgat gcccagcgtg
     2881 gtgtaacact cggggacggt atcgaccaga atccgcacgg cgcggatgtc gaacaggccg
     2941 tcgaagctga gttttttctt caccattttt ttgtaaatgg agtagatgtg tttcggacgg
     3001 ccggcgactt caaagtggat attgtatttt ttgagttccg tacgcaggat atcgaggaag
     3061 ttttcgatgt attcgaggcg ttcggtgcgt ttttcgtcca aaagcagggc gatttcgcgg
     3121 tatttttcgg gttcttgatg gcggaagccc aaatcttcga gctgccattt gagctgccac
     3181 acgcccaagc ggttggcgag cggggcgaag atgtcgaggg tttctttggc gacggcgcgt
     3241 ttttcggggc tgtcgggggc gttgcttaaa aattgcaggg tgcgcgtacg catcgccagt
     3301 ttgattaata cgacgcggat gtcggtaacc atcgccagca gcattttccg catggtttcg
     3361 gcttgctgcg cgcgctcttc ggaagtggcg aggctgtcca cccgggcgaa gtgggtgagc
     3421 ttctgcactt cgtccacacc tttgaccagc tcggcgacgg tgctgttgca gcgctcggaa
     3481 accaataggt tccaatcggg gacgtagcgt ccgatgtcgg caagcagggt ggcggcgacg
     3541 gcatcgggca gcaggtcgag ttcgtcgacc atttgcgccg cgccgaggaa gtggtcgggc
     3601 agcggctcgc catacggcgt ggcggcatcg gcaggataat gttcctgcgc cagcgaccat
     3661 gcggtaccga tgaggttttt atcgttgtcc ggcagagcgg cgcagtagct gtcgaaccat
     3721 tcgcgcaatt cttgcagggt cgcgctttgc gtgtcttgaa tcgggctgat ggcggtcatg
     3781 atgggatctt ccgttccgat aaaaacgagt tcattgtaac agcttattta tttacggtgc
     3841 ataggcggga agataaactt gtttcgggcg gcgccgggtg ttaaggtagc cggtaccgac
     3901 aggttgacga ggaatatttt gatgaaactg tatatttacg accattgccc gttttgcgtg
     3961 cgtgcgcgga tggctgccgg gctgtttggt gctgacgttg aagaagtcgt gctggcaaac
     4021 gacgacgagg caacgccgat cggtatgatc ggtgcgaaac aagtgcccgt cctgcaaaaa
     4081 gaagacggtt cgtttatggg cgagagtttg gacattgtgt gccatttcga ccgagaaggc
     4141 cgtctgaaag acgaagtccg ccccgagata caggcgtggc tggacaaggt aggcggatac
     4201 aacaacaaat tggttcagcc acgcttgatc aaaatcgggc tgcccgaatt tgtaacgccg
     4261 gaagcggtga aatatttcac agacaaaaaa gagaaaagca tcggcagctt ttccgccaat
     4321 ttgaataaaa ccgcccaata ccttgaacgg ataaacgcag atttgcaaga gttggagaat
     4381 ttgatggacg gtacttcaga cggcatcaac ggtggaatcg gtatggagga tattttggtt
     4441 ttccccgttt tgaggaatct gaccgtggtg cgcggcatcg cgttccctcg gaagacgatg
     4501 gattatttaa tcggtatgag tgaaaaatca ggcgtgccgc tttatttcga cagggcattg
     4561 taaaaccaaa aaaaagcggc agggcaatat gtccttccgc gttttttgga acgtttatgc
     4621 cgtctgagag ccagtcagac gaacatctgc acaaacgcaa tcaccagccg cacaatcggt
     4681 gcaataaacg cacccaaaac cccggtcagc ataagcagca ggataatcca cgtcccataa
     4741 ggttcgattt tgcggaacgc ttgcgaatat ttcgccggca ggaaggtgtc gatgaaaatg
     4801 ccgccgtccc aaggcaggat ggggatgatg ttgagcgcga acaaaatcgc attgatcaga
     4861 ataccgtagt ttgccatttg agccagcggc atctgatacg ccccgccggc atacggagtt
     4921 agcaccaaaa ccacgcccca cagaacagcc atcgccagat tcgacagcgg gccggacgcg
     4981 gcaatgcaac gccatgcaag gcgcgggttg cggaagttgc gcgaatcgat aggaatcgga
     5041 cgcgcccagc cgaacaggaa gggcgtgaac atcaaagtaa gcagcggtac gatgattgtg
     5101 ccgaccaaat cgatatgggg cagggggttc agtgtcagcc tgccgtattg ttcggcagtg
     5161 ttgtctcccc agtagcgcgc cgtatagccg cgcgccacct ccctgacggt aatggcgagc
     5221 agcacgggca ggacagcaag cagaaacacg cccaagtcaa aattttgaaa catggttttc
     5281 ctttgaacag atgtggtgtg cagcccgaaa cggctgacag acagaagcgg caatatatca
     5341 tctaacctga ttttattgga ataagaaatc agacggaatc attttttctg ccggcccgtt
     5401 atctgatgat ttgctgtaat accgcgttta aacacggttt gcctatgaat ggagcaggcg
     5461 ttcacatgac aacaaattat aaaaaatagg ttgcaaatag gaattgttgc tattattatg
     5521 cccgaattat ggataaagaa cgaattttaa cccccgcagt cgtgttttcc gtcgcactgc
     5581 tgcatttggc aatagtggca ttgctctggc aggcgcacaa gctgcccgtg atagagtcag
     5641 gaaatgttat cgaatttgtc gatttgggcg attttggcgg agggggcggc gcacccgaag
     5701 gcgcaggcgc gcctgccgcg cccgaaccgc aacccgcgcc cgatccgccc aaacctgtcg
     5761 aaccgcccaa gccggttttg aagccggcgg ttacaaaaaa ggcggatgcg gatattcagc
     5821 aacctaagga aaagccgaaa cccgaagaaa aacctaagcc cgaacccgaa ccggaagcga
     5881 agcctgcccc gaaaccggcg gaaaaaccgg cagagaagcc gtctgaaaaa cctgccgaac
     5941 attccggtaa tgcttctgcc aaagccggca gcgagcaggg taatggggaa ggtaagggaa
     6001 ccggtaccaa aggcgacgga acggggcgcg gagaaggcag cggtaaagga agcggcggtg
     6061 ccaaaggcga acacggggaa ggagccggcg gcagcggcgg cggaaccggt gtcggcagca
     6121 gcaaaggcaa tcctttacgc gccaacggca gcatcccgcg cccggcctat cccgcacttt
     6181 ctatggaaaa tgacgagcag ggtatggttg ttttgagcgt tttggtttct ccgggcggtc
     6241 atgtcgagtc cgtcaaagtg gtgaaaagca gcggtttttc ccgtttggac aatgcggcac
     6301 gcaaagcggc gcaaaacggg cattttcaag ccaatgcctg gacggagttt aaagtccccg
     6361 tcaagtttga attgaattag gcggttgtcg cggcagtttg cggcagcttg gatgtttgat
     6421 tggttttttc agtgttggaa tcattatgaa tttgaaatta gtgtttgaat cgggcgatcc
     6481 cgtcctgatc ggtgtgtttg tgttgatgct gttgatgagt accgtaacgt ggtgtctggt
     6541 tgtcttgcgc tgcatcaagc tgtatcgggc gcgcaaaggg aatgccgccg tcaaacggca
     6601 tatgcgcgat actttgtcgc tgaacgacgc ggtcgaaaaa gtgcgcgccg tcgatgcgcc
     6661 tttgtccaaa ctggcgcaag aggcattgca gtcttaccgc aactaccgcc gaaacgaagc
     6721 gtccgaactg gcgcaggctt tgccgttgaa cgagtatttg gtcattcaaa tccgcaacag
     6781 tatggcgcag attatgcgcc ggtttgatta cgggatgacc gcgcttgcct ccatcggcgc
     6841 gaccgcgccg tttatcgggt tgttcggcac ggtttggggg atttaccacg ccctgatcaa
     6901 tatcgggcaa agcgggcaga tgagtattgc ggcggttgcc ggcccgattg gcgaggcact
     6961 ggtggcgacg gcggcgggtt tgttcgtggc gattccggcg gtgttggcat acaacttcct
     7021 caatcgcggc acaaaaatac tgacccagga tttggatgcg atggcgcacg atttgcatgt
     7081 ccgcctgctt aatcaaaagg atagctgatt atggcatttg gttcgatgaa ttccagcgac
     7141 gattctccga tgtccgacat caacgttacg ccgttggtgg acgtgatgct ggtgttgctg
     7201 attgtgttta tgattactat gccggtgctg acgcattcca tccctttgga actgccgacc
     7261 gcgtccgagc aggcaaacaa gcaggacaaa cagcctaaag accccctgcg cctgacgatt
     7321 gatgcgaacg gcggctatta tgtcggcggg gattctgcaa gcaaagtgga aatcggggaa
     7381 gtggaaagcc gtctgaaagc cgccaaggag cagaatgaaa acgtgattgt ggcgattgcg
     7441 gcagacaagg cggtggaata cgattatgta aacaaagctt tagaagccgc ccgtcaggca
     7501 ggaatcacca aaatcggttt tgtaaccgaa accaaggcgc aataaagttc gacgcttgaa
     7561 cgaatgccgc ctgaaagtct ttcagacggc atttcttatg tttaatcagt tatatatagt
     7621 tgattaaaac aaaaatgaga cgacagagca ccgtctccat ttctgcgcag gcgggaatac
     7681 aaacatctga tttttctttt attttcaaac attactgaaa tgggtaagtc tggagtcctg
     7741 cctgcgcgag aataatgaaa atgtgcattt ctaattttta tagtggatta acaaaaatca
     7801 ggacaaggca acgaagccgt agacagtaca aatagtacgg aaccgattca cttggtgctt
     7861 cagcacctta gagaatcgtt ctctttgagt taaggcgagg caacgccgta ctggtttttg
     7921 ttaatccgct atacccactg taaaaacgga gaaggacaga ggacggcagt aaggtttgcc
     7981 gtcctctttt tcaagttgcg gtggaatgcc gcttgaaggt gtggtttaaa cgtttcggct
     8041 tgtaatcaga cgcgggcgtt gtccagccgc tctctgaagt tttccagatt cacacctgcc
     8101 gcttgggcgc ggcggatgat ttcgtcaacc ggcatacctt ctgccgcccg gcggaagccc
     8161 cacaggaggg agcagcgcgt accggtccgg caataggcaa ggacgggata ttcggcttgt
     8221 ccgatgagtt ggcggaaggt ttcgacatcg tgtttttgga tgtcgcgtgc ggtaacgggt
     8281 tggtgatgga atccggtaac gcctgcttgt tccagccact gtttgatttg ggcgaagtcg
     8341 ggttgcgatt cttcttcgcg gtcggggcgg ttgcagatga cggttttgat gccaagttgc
     8401 gcgatttgtt ccgcatcggc tttggtcagt tgcggggaga tgtagagatg ttcgtcaagt
     8461 ttgagaatcg ccattttgtt tcctttgctt tgcggttggt gttcagacgg cattttgtgt
     8521 gcaatgccgt ctgaaagttt aaccgaacaa ccagttttcc aacaggttgc gatctgtgat
     8581 gtgtaggatg gcagtatccg ccgccgttgc ctgaaccgtc aagacggttt gacgcaatat
     8641 gcctgcacgg aagaagtgga tattgatttt tgccctgacg tggtatcggg cccattgtgc
     8701 ggcaaagtcg gtgcaggcat aaccgtctaa agcaatgatt ttgtcttgcg ggcacagtgc
     8761 cgcagattcc gcacttccgc cgttgaagac atgggtcagg acgatatggt cggcgttttg
     8821 tttgaagcgt gcgccgaaat cgcccgtcgg cgggatgggg aaaatgtgtt ccgcatatcc
     8881 gccgccgtgt tgtcggggaa gcggcaggaa ggtcagtttc acgcctgtgg ttgccaggca
     8941 ttcggcaagc ggcaaatctt cggtgctgta caatgccgtc tgaaaaaaat ctgtcaaatc
     9001 caagccggta atttcctgac aacggatttg ccagtgtttt tccggaatac ccgagtgtgt
     9061 gtccctccac tcccgataga gtttgtccat taccgtatca agggaatgtc tgccgttgct
     9121 tcggttgcgt attatcagat caaggcacaa tgcggcaagt gcgccttttt ggtagtagct
     9181 gacgatggcg ttggggctgt tttcatccgg tttgtaaaat ttgttccacg cggtaaaact
     9241 cgattccgcc aaggtctgcc tcaaacggcc gcgggtttgt tgtacgcgcg taatgccttg
     9301 tgctagcagg tttaaataaa attcgggcga gatggtgcgg ctgcgtgcca aaaacaaatc
     9361 gtcgtaatag gatgtaatgc cttcgaatgc ccatagttgt tcggtatagt tttctttgtc
     9421 gaggtcataa ggggcgaatg cggcaggttt gatggatttg acgttccacg cgtgaaaata
     9481 ttcgtgggag aaaagtccga gcaatgtggt gtaggcatcg tcggcatcgg tcataccgta
     9541 cggcggaagg ctgtggcgat cggcgagcag tgcggtgctg tcggtgtgtt ccaaaccgcc
     9601 gtaaatatgg tcgccgacgt ggagcaggaa caaatatttc tcaaatgggg caggggagga
     9661 gaacatcgcc agttctgttt cgcagatttt tttgatgtcc gaaaccagcc tgtcgcggtc
     9721 gaaatcggga tatatgccgt ttaaggcaat cgtgtgcgga atgcctgccg cctcaaaatc
     9781 taaaaattca atcaagccca tctcgacagg tcggtcaatc aattcgtcat aagatgccgt
     9841 ctgaaagaca agcctccctg tttccggcag cgttgtggca atacgccatt cgggtggaat
     9901 gcctgtcaat tccaagcggt gcggcagcgt ttccgttcct tcgactttca aaaacaggca
     9961 cgatccgtca aaaaaaccgc gttctgtcgt caggaaagaa cctcgaaccg acaaatcgaa
    10021 tgcatatacg gtgtagcgga tttgccactc gccgcgtacg gcggcagtgt gccagcggtt
    10081 ttttgcaatt tgttcgagcg gcatggacgt gccgttgcag gatgcatgga tagaagtgat
    10141 gtggcgggaa aaatcccgaa tcagatagct gcccggcacc caattcggaa ggcttatttc
    10201 tataggaaaa tcattatctt gtgtgaattt cagcaggatg tgccattcat ggtctagagg
    10261 ggacggggct attttataat gaatcataac tatcaaatat atcgttaaac gggatggatt
    10321 aaatgccttt aaaatgcagc ttaacctaca tcaataaagt gccacaaaaa gggaaaattg
    10381 gaaacaaaag caatggcggc cgggttgtaa tttattataa caatatgatt tatatgtaat
    10441 aaaaaacaga ttaagcaaag ggttcgccgg cggcgcgaat gtaattccgc ttggcgtaat
    10501 aaggcagata tgttaaattt agaaaaaatt acgcttgccc ggattgcacc ttaaacaggc
    10561 tgcggaagcg gcaggataaa aatctttatt cccataattc atggagactc tcatggacac
    10621 acaaacttac aactacaaag tggtgcgcca gttcgccatc atgactgtag tttgggggat
    10681 tgtgggcatg ttggtcggcg ttatcgtcgc cgcccagctt tttgctcctg ccctcgactt
    10741 gtctaatatc ggaccttggt tccacttcgg ccgcctgcgt ccgctgcaca ccaatgcggt
    10801 tattttcgca tttggcggtt gcggtctgat cggcacatca tactacgttg tccaacgtac
    10861 ttgtaatacc cgtcttttcg gcggttggct gccggcattt accttttggg gttggcaggc
    10921 ggtaatcgtt gccgccgtcg tcagcttccc tatgggttgg acccaaggta aggaatatgc
    10981 cgaactggaa tggccgattg acattctgat tactttggtt tgggtggctt acgccatcgt
    11041 attcttcggt acgattgcca aacgcaaggt aaaacacatt tacgttgcca actggttcta
    11101 cggcggcttt attttggcgg tcgcactttt gcacatcgtc aacaatatca gcatcccggc
    11161 cggtttgatg aagtcatacc ccgtctattc cggtgcgatt gatgctatgg ttcaatggtg
    11221 gtacgggcat aacgccgtgg gcttcttcct gactgccggc ttcttgggta tgatgtacta
    11281 tttcgtaccc aaacaagcag cccgccccgt ttactcctac cgcctgtccg tcgttcactt
    11341 ctgggcgttg attttcacct atatgtgggc gggtccgcac catcttcact acactgcgct
    11401 gcctgactgg acgcaatctt tgggtatggt tctgtccttg attttgttcg caccctcttg
    11461 gggcggtatg attaacggca ttatgacctt gtccggcgcg tgggacaaac tgcgtacaga
    11521 cccgattctt aaattcctga ttgtatcctt gtccttctac ggtatgtcta cctttgaagg
    11581 cccgatgatg tcgattaaaa cggtcaatgc attgagccac tatacggact ggaccgtcgc
    11641 gcacgttcat gcgggtgcgt tgggctgggt aggctttgta accatcggtt ccgtctatta
    11701 catgattccc cgtctgttcg gcaaagagca gatgcacagc accaagctgg tagaggcgca
    11761 tttctggatt gcgactatcg gtgtggttct gtatatcgcc gcgatgtgga ttgcaggtgt
    11821 gatgcagggt ttgatgtgga gttctctgaa cgatgacggt acgctgacct actcgtttgt
    11881 cgaatccgta aaacgcacca tgccttacta catgatccgt tttgcaggtg gtttattgta
    11941 tctgagtggc atgtgcatta tggcgtacaa cgtgtaccgc acagccatcg gtggtaaagc
    12001 agtcgatgcc gaaatccctg cggtttccca aacacagcac cactaaaata ctaagaaaga
    12061 taggctacca aaatgaaatt acaacaattg gctgaagaaa aaatcggcgt tctgattgtg
    12121 ttcacgctgc ttgtagtcag tgtcggtctg ttgattgaag ttgtgccctt ggcctttacc
    12181 aaggcggcaa cacagccggc gccgggcgtg aagccttaca atgccctgca ggttgccgga
    12241 cgcgatattt acatccgtga gggctgttac aactgccact ctcaaatgat tcgtccgttc
    12301 cgtgcggaaa ccgagcgtta cggtcattac tctgttgccg gagagtcggt ttacgaccat
    12361 ccgttccaat ggggttccaa acgtaccggt cctgatttgg cacgtgtggg cggccgctat
    12421 tccgacgaat ggcaccgcat ccacctgctg aatccccgtg atgtcgtgcc tgagtccaat
    12481 atgccggcat tcccgtggct tgcacgcaat aaagtcgatg tcgatgcaac cgttgccaac
    12541 atgaaggctt tgcgtaaagt aggtactcct tacagtgatg aggaaattgc gaaagcgcct
    12601 gaggctttgg caaacaaatc cgagctggat gctgtagtcg cctatctgca aggattgggt
    12661 ctggctttga aaaacgtaag gtaacatcat ggatattaac ggtattcgcg ctcttttcac
    12721 ggtatggatc tttatctgtt tcctgttagt actctatatc gtcttcaaca ggcggaataa
    12781 gaaaaactac gatgatgccg caaacagcat ttttgctgaa aaccaagatg cgcaagataa
    12841 gaaaagcgaa aaccgttaat attgtgataa cggagcaaaa caatgaacgc aacatcccaa
    12901 tttaccagta atttctggaa tatatatatt gcagttattg tcttactgag ctttatcgct
    12961 ttggcttggc tgctgctgtc tcaaaatgtt gtcaaacgtc cgaagaaggg cgaagaagta
    13021 caaactacgg gtcacgagtg ggacggcatt gccgaatacg acaatccgct gccccgctgg
    13081 tggttttggc tgtgtgtttt gacgtggctg ttcggtatcg gttatctggt tatgtatccg
    13141 ggtgtcggcg actacaaagg tctgctgaaa tggaccagcc ataaccaata tgaaaaagag
    13201 gtcaaaaaag ccgatgagca atacggcaaa ctgtatgcca agtttgcgga tatgccgatt
    13261 gaaaaagtgg caaaagaccc acaggccaag caaatcgccc aaaacctgtt taacacttat
    13321 tgtatccagt gccacggctc tgatgccaaa ggttctaaag gttttccgaa tctgaccgat
    13381 agcgattggt tgtggggcgg cgatcccgat aaaatccacg agaccatcga aaaaggccgt
    13441 gttgcgacta tgcctgcctg gggtcctgct ttgggcgaag aaggcgtgaa agacgttgcc
    13501 cattatgtga tgtccctttc aaaacccaaa ggtcagtatg atgaggaacg tgccgcgcgc
    13561 ggacaagcct tgttcagcgg tccgcctgcc aactgtttca cttgccacgg cgataaggga
    13621 caaggtatcc aaggtttggg tccgaacctg actgatgacg tgtggttgtg gggcggtacg
    13681 caaaaatcca ttatcgaaac cattaccaac ggtcgcagca gccaaatgcc cgcttgggga
    13741 catttcttgg ataaagacaa actgcatatc atgactgctt atgtatgggg tctttccaat
    13801 aaagacggta aagctccggt gaaaaaagcc gagcctgcac cggcggcaga acccgcacca
    13861 tctgctcctg cagaagcagc acaagccgct tctgaagcca aacctgccgc agcagaacct
    13921 aaagccgagg aaaaagccgc acctgccgcc aaagcggatg gcaaacaggt ttatgaaacc
    13981 gtttgtgccg cctgccatgg caatgcaatt ccaggtattc cccatgtagg caccaaagcc
    14041 gactgggccg accgcatcaa aaaaggtaag gacacgttgc acaaacacgc gatcgaaggt
    14101 tttaatacga tgcccgcaaa aggaggtcgc ggcgatttga gcgatgatga ggtcaaagct
    14161 gcggttgact atatggtcaa ccagtcaggc ggtaagttct aaccgttgtt ttccgacttt
    14221 gtcggatgca gaaatccgga ttcatatcga atccggattt tttattttga acggaaatcc
    14281 tgtttttgat ttatgccgtc tgaaagtggg gattttgatg cttaagcagt cagaccgact
    14341 gatatttttg tatggtttaa agaatggtgc agttgggtgt cggacagcat tgtttgatgc
    14401 tttcagacgg catataggtt tgtttcttca aaattgaatc ttttttcata tgttgcaaaa
    14461 aaattgattt tagtcataaa gaattagtct ttattgttta taatcgcgca tcatcacgat
    14521 agccttaatt tcaaaaggac gattaatgga tacacaaatc aaaactgaag ccggtcagca
    14581 gaataaccgg cattatctga gcgtttggag gtggcatttt tatgccggtt tattggttat
    14641 gcccttcttg accctgcttg ccgttacagg tttgggtatg ctgttgtttg ccaatattac
    14701 cggtaaagag ggagagcgta ttcacgtttt ccctcaggca gtggtgcaac ccctctctgt
    14761 tcaggcagaa gcggcacgcg gtgccattaa tccggagact tcgtccgtcg tccaatatat
    14821 tgcaccgcgt gccgatgata tggttgctgt gttccgtgtc aacaatgagg gtaaggcaac
    14881 catggttgcg gtcgatcctt atacggcaaa agtggtcaac accatgccgc gcaatcaggg
    14941 ttggtatcac actatggatg aaatccacgg cgatatgatg ctcggtgcgg caggcgatta
    15001 tcttttggaa acggcagctt cactgaccat tattatggtt gtcagcggct tgtacctttg
    15061 gtgggcgaaa cagcgcggca ttaaagcgat gctgctgccg ccaaaaggca gggcgcgttc
    15121 ttggtggcgg aatctgcacg gcgcgtttgg aacttgggta tcgttgattt tactgttgtt
    15181 ctgcctgtcg ggtattgctt gggcaggtat ttggggcggc aagttcgtgc aggcttggag
    15241 tcagttcccg gccggcaaat ggggtgtcga accgaacccc gtttcagtcg tgccgaccca
    15301 cggcgaggta ttgaatgacg gcaaggttaa ggaagtgccg tggattttgg agcttacgcc
    15361 tatgcctgtc tcagggacga ctgtgggtga aaacggcatt aaccccagcg agccgatgac
    15421 attggaaacc gtcgaccgtt tcgcgcggga aatcggtttc aaagggcgtt atcagttgaa
    15481 tttgcccaaa ggcgaggacg gggtatggac tttgtcgcag gattctatga gttatgacat
    15541 gatcagcccg tttgccgacc gcacggtaca tatcgaccag tacagcggca agattcttgc
    15601 cgacatccgt tttgacgatt acaacccgtt cggcaaattt atggcggcaa gcattgcgct
    15661 gcatatgggg actttgggct ggtggagcgt gttggcgaac gtcgtgttct gccttgccgt
    15721 gatttttatc ggcatcagcg gctgcgtgat gtggtggaaa cgccgtccgt ccggcgtggc
    15781 gggcattgtt cctccggcgc aaaaaatcaa actgcccgtc tggtgggcga tggcattgcc
    15841 gctgctgttg attgcactgc ttttcccgac cgcgctgctt gccattgccg tgatttggct
    15901 gttggatacc ttgctgctgt cgcggattcc tgtgttgagg aaatggttta aatgacccga
    15961 tgccgtctga agcgttcaga cggcattttg tttgcgggcg gcttgcccga gaagctatat
    16021 aattccgcat cactgtcatt ttgaaaaagc aagccatgtt ttacctgtac caatccaacc
    16081 gcctcgaatc gctcgctgca ctgtttgccc gcattcaaaa agtcaaaccg ctgaaatgtg
    16141 ccttgcagcc cgaacagatt gttgtgcaga gtcaggggat gcgccgctac ctcaacacct
    16201 gcctcgcccg cgatttgggc gtggcggcga atttggcgtt cagcctgccc gccggcctga
    16261 cgtggaagtt gatgaaaaaa ctgattcccg gtattccgga actcagcccg tttgcacccg
    16321 aagtcatgcg ctggcggctg ctggatttgt tccgcagcgc agaatttcaa aacggagcgg
    16381 aatttgaaga tgtgaggaat gtgctgcaag actatctggg cagcggagaa tcggcggatt
    16441 accagcttgc gggacagctt gcagacatat tcgaccaata cctcgtgtac cgtcctcagt
    16501 ggatagacgc ttggcagcag ggcaggatac tcggtttggg cgacgacgaa atctggcagt
    16561 ccaaactgtg gcgttacctc gacgacggca ggcagagcgc gccgcaccgt gtcgcgttgt
    16621 gggaaaaact gcttgccgcg ttggacaagg ataagctgcc cgagcgttat ttcgtgttcg
    16681 gcatttccac gatggcgccg atgtatttgc agcttttgca caagctgtcc gaacattgcg
    16741 acgtgttcgt gttcgcactc aatccgagcg ggatgcattg gggcaacgtc atcgaagcgg
    16801 cgcaaatcct caaaggcggc ggcgatcccg atctgacgca ggcagggcat ccgctgcttg
    16861 cctcattggg caagcagggg cgcgactttt tcgatttttt gaacgaaatg gaaatagaag
    16921 gcgaaacgcc ggtatttgaa gaaggcggac gcgatacgct tttgcacgcc ctgcaaaccg
    16981 atattcaaaa cctgaaaatg ccgtctgaaa cggtgggaag cgtcaacacg ggcgacggct
    17041 cgatacgcat cgtctccgca cacagccctt tgcgcgaatt gcagatactc aaagacaagc
    17101 tgttgaaaat tctgcatgaa catcccgatt ggcagccgca tgatatcgcc gtattaaccc
    17161 cgaatatcga accctatacg ccttttatcg aagccgtgtt cggacaggcg caacccggcg
    17221 cgcaggcatt gccgtattcc gtttcggacg tgaaaatcag ccgccggcaa ccgttttttt
    17281 atgcgttgga gtgcgtgtcc gacttgctgg aaagccgttt tgaagtcggc aaagtgctcg
    17341 cgcttttgga aaccgcgccc gtgttgcgcc gtttcggact gaccggggac gatttgccgc
    17401 ttttgcacga tatggttgcc gatttgaacg tccattgggg attggacgga gaaatgcgcg
    17461 gcggcacgga tcagctgttt acctggaagc aggcggcaga ccgtatgata ttgggctgga
    17521 tgctgcccga aggcggcaac ccgatgtggc aggatgtcag cgcgtggtat gccgacgtga
    17581 atcaaaccgc catgttcgga cgttttgccg ccttcctcga aaccctgtcg gatattgccc
    17641 ggatatggcg gcaacctgcg acggtcggcg aatgggtggc gcgttgccgg gatttgcttg
    17701 aaacattgtt tcaagccggg cccgatgacc aaaaggcagt ccaaaacctc gaaaacgaat
    17761 gggtcaaatg gcaggaagag agcactttgg ctgggttttt cggacagttg ccgcaacaaa
    17821 ccgtcatccg ccatatccga cgtttcctcg acagcgaaag cgaggcaggc tttttacgcg
    17881 gcggcatcac cttttgcagt atggtgccga tgcggagcct gccctttaaa gtcatctgcc
    17941 tgttgggttt gaacgacgga gattttcccc gcaataccaa agccgccgta ttcgacctgg
    18001 ttgccaaaca tcccgccaaa ggcgaccgcg cccgccgcga cgacgaccgc tacctcttcc
    18061 tcgaagccct catcagcgcg cgcgaaatcc tgtatttgtc ctacatcggg cgcgacatcc
    18121 gcaaagacga agaactggct tcgtcttccc tgttgggcga actcatcgat accgttgccg
    18181 ccatgaccgg cacaaacagc cgccaacttg cacaaaattg gatagaacag catccgctgc
    18241 aagccttctc gcgccgatat ttccaagaag gcggacgttc agacggcata ttcggcacgc
    18301 gtaccgacta cgccgccgcg ctcggacaaa cgcccgaacc gccgcaaccc tttttcgacc
    18361 aacccgtaga aaacgccgaa cctgttgccg aaatcggaca ggacgaattt atccgcttct
    18421 ggcgcaaccc cgtcaaagta tggctgcagc agcttgcgtg gagcgaaccc catatcggcg
    18481 aagcctggga gcctgccgaa cccttcgagc cgcaacacgc ggagcaaatc gccgaaacct
    18541 atatcggggc gcgccgcgaa ggacaagatt tttcccaaac cgccgcccgt atcgcggcgg
    18601 aaagcctgct gccttccgga gaattgggaa aactttggca gcagtccttc caaaccgccg
    18661 ccaaacaaat cgacacggcg gttttaaaca gccccaaact gcccccgttc ccatacgcca
    18721 taccgtcgga cgggcaaatc ctgaccggca gtttgagcaa tttataccaa tgcggacaag
    18781 tgttttatgc ctacggcaaa cccaatgcgc cgcaacgtgt ggcttttttg ttggaacacc
    18841 tgatattttg cgccgttatg ccgtctgaag ccgaaacgcg gcaaaccttt atcgtccaat
    18901 tcggagaaac agaagtattg gcggaaatcg cgcaagacag ggcattgcag ctgttgtcgg
    18961 aatggatggc gtttttcaat atcgggcaaa accgcccgct gccgtttttt gccaaaacct
    19021 cgcttgccgc cgccgaagcg tttgcccaaa aacaagattg ggaagccgcc ctgaaaaaag
    19081 cccgaaccgc ctattacggc agcaaagtca gcaaagggca gaaagactat accgaagtcg
    19141 ccctcgtgtt cggcaacgca agccaaaacc cgctcgaaca gcccctgttt gaaaacctcg
    19201 cccgcctgct cgccgacacg cttgccgcag cggaaaagag ggaagggacc ggagaagcct
    19261 gacgggatgg aaaggcaaat gccgtctgaa aacagaaaaa aggttttcag acggcatttt
    19321 gtttatcgtg tttaaggcac cggatagaat gtgggtgcgc cgggaccgac gggcaggccc
    19381 aaaacaaata cccaaatgca gaataaggcg atccatgcaa ttaagaagaa agcggaatac
    19441 ggcaacatca tagaaatcag agtacccacg cccgcatctt ttttgtattt gattaccgtc
    19501 gccataatca gcccgaaata actcatcatc ggcgtaataa tattggtaac ggaatcaccg
    19561 atgcggtaag cggcttgaat gacttcgggc gcgtagccgg ccaacatcag catagggacg
    19621 aaaatcggcg cagttaccgc ccattgcgcg gaggcggagc ctatcatcag attgataaaa
    19681 gcacaaatta aaataaaacc gataaacaac acgctgccgc ccaagccgac ttcttttaag
    19741 aacaccgccc ctttaacggc aatatattgc ccaatattcg tccaattaaa aaatgcgaca
    19801 aactgtgcgg caaaaaagat gatgaccaaa taaagcccca gagtactcat cgattcggcc
    19861 atcgcattaa cgacttcccg ttcgccgcgc aaacttcggg ttatccggcc ataaacaatg
    19921 cccggcagcg caaacaacaa gaaaataaaa acaacaatcg attttaaaaa cggcgaaccg
    19981 gcaaccaatc ctgtttcagg atgacgcaaa ataccgtcgg cagggacgat gctccaagcc
    20041 aatagggcgg ataaggcaac aaacaccacg cctgcccaaa ttaatccttt atattccaaa
    20101 ggcgtgattt cattggaatg ccggatgtct ttttcttctt gtgacaaatc tgactgataa
    20161 gggcccaatt gcggttcgac gattttttca gtaacaaaat aaccaatcaa agcaatcaca
    20221 aacgtactgg ttaccataaa aaaccagttg gcttcagggc ctacgacgta gtcgggatgg
    20281 atgatttgcg ccgcctgttg ggtaatgcct gccaagagcg gatcgagtgt gcctaagaac
    20341 agattggccg aataaccgcc ggaaacgccg gcgaaagccg cagccagacc ggcgagagga
    20401 tggcggccga gcgaatgaaa gatgacggcg gacaaaggga ttaggacgac atagcccaat
    20461 tcagaagccg tattggataa aatccctgta aaaacaacca taaaagtagt gagtttgcgt
    20521 ggggattttg taagcaataa gcgcattaat gcagaaatca agcccgattt ttccgcaatc
    20581 cccacgccca ataaagaaac caacaccgtt cccaacggcg cgaaaccggt gaaattttta
    20641 acggtatgcg tcaggatttt gatcaaaccg tcggcatcga gcaggctgac aacgtgaatc
    20701 aaaccgtcat cggcacgtcc ttttgcccca acaggacgcg gatcggggac ggatagtccg
    20761 aaatgcgcac cgacggcaga ggcaatcagc aataacacaa tgaaaataat aaaaagcgta
    20821 accgggtgcg gcaacatatt gcccagccat tcgactgtgc gtaaaaatcg tccgctacga
    20881 cgcgcgtcgg tttgactcat ctgcttctcc ttaaaaaaat atattgagtg cattaaattt
    20941 ttgaatttaa catgaagtgt tttgtaagca aactaaaata atcgtatatg tatataaccg
    21001 taatagatta aaacggaaaa tgcccacatc gttattctca taaaggcggt aatccgaaaa
    21061 cttgacgtta aagccttatc aggaatgact ggaactcaaa aaaacgaatt cccgcctgcg
    21121 cgggaatgac gggattttcg gtttttgttt gcaagaatgc cggagaaaaa tgca
//
LOCUS       DAXFZR010000036        21059 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000036 DAXFZR010000000
VERSION     DAXFZR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21059
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.036"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            175..2202
                     /locus_tag="ACFJAS_001111"
     CDS             175..2202
                     /locus_tag="ACFJAS_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC3982383.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2292..3845)
                     /locus_tag="ACFJAS_001112"
     CDS             complement(2292..3845)
                     /locus_tag="ACFJAS_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC3982384.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIHKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFEGNK"
     gene            3896..4024
                     /locus_tag="ACFJAS_001113"
     CDS             3896..4024
                     /locus_tag="ACFJAS_001113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982385.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAACRRFAPVLPMSDLNAV"
     gene            complement(4097..5290)
                     /locus_tag="ACFJAS_001114"
     CDS             complement(4097..5290)
                     /locus_tag="ACFJAS_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC3982386.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGEAFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRYNGNWQSLNSRSAAADFPLGGILAQTGSGGSMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5287..6270)
                     /gene="patB"
                     /locus_tag="ACFJAS_001115"
     CDS             complement(5287..6270)
                     /gene="patB"
                     /locus_tag="ACFJAS_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC3982387.1"
                     /translation="MKNFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQGTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6281..7717)
                     /locus_tag="ACFJAS_001116"
     CDS             complement(6281..7717)
                     /locus_tag="ACFJAS_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC3982388.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGILAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWLITFHFVCLTFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIQISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(7847..9012)
                     /locus_tag="ACFJAS_001117"
                     /pseudo
     CDS             complement(7847..9012)
                     /locus_tag="ACFJAS_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9453..9665
                     /locus_tag="ACFJAS_001118"
     CDS             9453..9665
                     /locus_tag="ACFJAS_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC3982389.1"
                     /translation="METLLLDIVGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            complement(9644..9805)
                     /locus_tag="ACFJAS_001119"
     CDS             complement(9644..9805)
                     /locus_tag="ACFJAS_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982390.1"
                     /translation="MSEGTIARKFNQFILPVQLNQMPPEGAERASDGIGETQRLQTAF
                     LPLYFNAAS"
     gene            9813..11391
                     /locus_tag="ACFJAS_001120"
                     /pseudo
     CDS             9813..11391
                     /locus_tag="ACFJAS_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            11473..12570
                     /locus_tag="ACFJAS_001121"
     CDS             11473..12570
                     /locus_tag="ACFJAS_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC3982391.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12684..13799
                     /locus_tag="ACFJAS_001122"
     CDS             12684..13799
                     /locus_tag="ACFJAS_001122"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC3982392.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTAGNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNPASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVPPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSFAEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            13867..14379
                     /locus_tag="ACFJAS_001123"
     CDS             13867..14379
                     /locus_tag="ACFJAS_001123"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC3982393.1"
                     /translation="MPSERVSASGGIALEQGWDMKKPKILFVCLGNICRSPMAEYILR
                     RRAAEAGIPLETDSAGTSGWHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFD
                     YIIAMDGKNLSELEKTFGRRPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAG
                     CRALLEKISK"
     gene            14456..14932
                     /locus_tag="ACFJAS_001124"
     CDS             14456..14932
                     /locus_tag="ACFJAS_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC3982394.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14920..15846
                     /locus_tag="ACFJAS_001125"
     CDS             14920..15846
                     /locus_tag="ACFJAS_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3982395.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15911..16465
                     /locus_tag="ACFJAS_001126"
     CDS             15911..16465
                     /locus_tag="ACFJAS_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC3982396.1"
                     /translation="MKPKFKTVLTALLLAVCLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGLYQNVPIQPVKIRRVVVE"
     gene            complement(16599..17558)
                     /gene="ttcA"
                     /locus_tag="ACFJAS_001127"
     CDS             complement(16599..17558)
                     /gene="ttcA"
                     /locus_tag="ACFJAS_001127"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC3982397.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(17650..18513)
                     /locus_tag="ACFJAS_001128"
     CDS             complement(17650..18513)
                     /locus_tag="ACFJAS_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC3982398.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQAGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAE"
     gene            complement(18548..18910)
                     /locus_tag="ACFJAS_001129"
     CDS             complement(18548..18910)
                     /locus_tag="ACFJAS_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC3982399.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            18985..19464
                     /gene="ybaK"
                     /locus_tag="ACFJAS_001130"
     CDS             18985..19464
                     /gene="ybaK"
                     /locus_tag="ACFJAS_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC3982400.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            19486..20178
                     /locus_tag="ACFJAS_001131"
     CDS             19486..20178
                     /locus_tag="ACFJAS_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC3982401.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNLQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIIQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLIIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            20182..21057
                     /locus_tag="ACFJAS_001132"
     CDS             20182..21057
                     /locus_tag="ACFJAS_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC3982402.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
BASE COUNT         4841 a         5291 c         6001 g         4926 t
ORIGIN      
        1 cccggcagct aaaaatcctg cgggatcggt gtggaattta gggataatct agtacagccc
       61 catttcggcc ggtttgtgca tttcgtcatc cggaagccgg tattttgccg gcaggttttg
      121 actactttgc ccgatgaccg ggtttgtctg tttttaacaa aggagtgaga aggtttgtcc
      181 ctgtctgaat ttatagaacg ccgaacgtca tttaatccga tggttatttt gacgactttg
      241 ttttttgtgt gtgttttggt ggtattggtt ttaaccgtgc cggatcaggt gcagatgtgg
      301 ctcgaccggg caaaagaagt catttttacc gagttcagct ggttttatgt tttaacgttt
      361 tccatttttc tgggttttct gctgatactc tcggtcagcg gtttgggaaa catcaggcta
      421 ggacgggatg aagatgtgcc ggaattcggc ttcctgtcgt ggctggcgat gctgtttgcg
      481 gccgggatgg gcgtgggcct gatgtttttc ggcgtggcag agccgttgat gcattatttt
      541 tcggacatta cggtcggcgc gccggaacac aggcagcagc aggcattgct gcacacggtg
      601 ttccattggg gcgttcacgc ctggtcggtg tacggtacga ttgcattggc tttggcttat
      661 ttcggtttcc gctacaaact gccgcttgcc ctgcgttctt gtttttaccc cctgttgaaa
      721 gaaaaaattt ccggaaggtt cggcgatgcc attgatatta tggcgttgct tgctactttt
      781 ttcggcatca tcaccacatt ggggttcggg gcttcgcaac tgggcgccgg attgcaggaa
      841 atgggctgga ttgccgaaaa cagcttcggc gtgcaggtct tgattatcgc cgccgtaatg
      901 tccctcgccg tcgtttcggc aatatccggc gtggggaagg gcgtgaaggt gttgagcgag
      961 ttgaacctgg gccttgcgtt tttgctgctg ttttttgttt tggcggcggg ccccactgtt
     1021 tacctgttgt cggcattcgg cgacaacata gggaactacc tcggaaatct ggtgcgcctc
     1081 agtttgaaaa cttatgcgta cgaacgggaa cacaagccgt ggtttgaatc ttggacggtg
     1141 ctttattggg cgtggtggtg ttcttgggcg ccgtttgtgg gtttgtttat cgcgcgcatt
     1201 tcaaaggggc gcaccatccg cgagtttgtc ttcggggttt tgctcatccc cggcctgttc
     1261 ggcgttttgt ggtttaccgt cttcggcaat acggcgattt ggctgaatga cggggttgcg
     1321 gggggaatgc tcgaaaagat gacctcctct ccggaaacgc tgctttttaa attctttaat
     1381 tacctccccc tgcccgaact gacgagcatc gtcagcctgc tggtcatttc cctgtttttt
     1441 gtaacttctg ccgactccgg gatttatgtc ctgaacaata ttacctctcg ggacaaaggc
     1501 ttgagcgcgc cacggtggca ggcggttatg tggggcgtgc tgatgtctgc cgttgccgtt
     1561 ttgctgatgc gctcgggcgg actcggcaac ctgcagtcta tgaccctgat tgtttccctg
     1621 ccgtttgccc tgctgatgct gataatgtgt ttcagcctgt ggaaaggctt gagtgcggat
     1681 aagaaatatt ttgagaccag ggtcaaccct accagtgtat tttggacggg cggcaagtgg
     1741 aaagaacggc tggtgcggat aatgagccag acgcaggagc aggatatttt aaaattcctc
     1801 aaacataccg catcgcccgc tatgcacgag ttgcaacggg agctttcgga agaatacggc
     1861 ttgagcgtcc gggtcgataa gatgtttcat caggacgagc ccgcaatcga gttcgtcatt
     1921 cggaaagaga cgatgcgcga ttttatgtac gggattaagt ctgtcgggca ggatgtatcc
     1981 gaccagttga ttaacgacgg caagctgccg catatccggc accagacaac ttacaaaccc
     2041 tacgcttatt ttttcgacgg gcgcgtcggg tacgatgtgc agtatatgaa caaggacgag
     2101 ctgattgccg acattttgaa aaactacgaa cgttatttga tgttgttgga tgatgtcggt
     2161 caggaactga tggcgcacga gcaggtggaa ttggcagagt aaatatcctc gctccggcgt
     2221 ttgccaaaaa aatgcggtga atcagattca ccgcattttt ttgccggacg gggcttcgga
     2281 cggcacggcg ttcatttgtt tccttcaaac tgctccttca gcacccgttt caatactttg
     2341 cccgtagcgt tgcgcggcag cccgtcttta aagtggatct gtttggggat tttgaaattt
     2401 gccagcacgg tacgcaggtg gcggcggatt tcgtcctcgc ccaaatccat accttccttc
     2461 aattggacga aggcgacgat ttcctcgtcg gcataacggt ctttcacgcc gatgacggcg
     2521 gcggcttcga cggcatcgag tttgtggatt tcttcttcga tctcgcgcgg atagacgttt
     2581 tgacctttgg aaataatcaa atcttttttg cggtcgacga taaagataaa gccgtcctcg
     2641 tctatggtaa cgaaatcgcc cgttttcaac cagccgttga cgatggtttc atcggtggcg
     2701 gcaggcatat tgaggtagcc ccgcatcacc gaaccacccc tgacgatcag ttcgcccact
     2761 tcgccgcgcg gcacttcgac caattcttca tcgacggctt tggcttccaa accgggcagg
     2821 gggatgccga cgctgcgggc tttttgccgt tcgggcgtat tgacggcgac gacgggcgag
     2881 gcttcgctca gtccgtagcc ttccagcaat ttggcgcggg ggaacttggc tttaaaatcg
     2941 aggatggttt gttccgccaa aggcgcgccg ccgctgataa acaggcggat gcggttgaac
     3001 catctgaaat accaagggat tttcgccttg ctcatcgcgg tgtaaatcgc gggtacgccc
     3061 aaaaacacgg ttgcgcgttt gagcagggcc tgtttcaaaa cgttggaaaa ggggaaaacg
     3121 gatttgacca aaataatcga acacgccata taaatcggca gcagtaccat agccgtcagc
     3181 gtgaagctgt ggaacatcgg caggaaaacg ataaagcggt cgcgtttgga aattttaaag
     3241 atgcgttcga tgccgttcag gttggcgaac aggttggcgt aactgattag cgcgcctttg
     3301 ggatgccccg tcgtgccgga ggtgtagatg atgtgtgcca aatcatttat ccggggttgg
     3361 cggcccaagt cgggtttttc ggggaagcgg cgcacgtttt caaaaaaggc atcgccttcc
     3421 gccgtttcgc cggccggccg gcttttgtcc gtccaaatga ttttttcgac gggcgtttgc
     3481 gccttcaggc ccgccaattc ttttgacagg ccggccgagg cgaacaggaa gcgcgccttg
     3541 cagtcattca ggatatacgc gtattcgctg tttttcaaaa atgtgttcat cggtacggcg
     3601 accgcgccga tggcggagac ggcgaaatag gcggtaataa attctgtgga attggaaacc
     3661 gccagcgcga ccgtgtcgcc gaacttcacg ccgatatttt gcagatacgc cgcgacggct
     3721 tcggcttcct gcttgagcgc gcggtaggcg gttttttcct tgccgtcgaa cactgccgtg
     3781 ccgttgccgt ttttgcggca ggcggcggcg agcatttcgt agaaattggc ataagtccgg
     3841 ttcatgaaac ttcctttatg gttgcggacg gcgaacgccc tcatttgaaa acaatatgcc
     3901 gtctgaaaac ggaaggcggc agtttatcat ctttgccttc agacggcata tcaaacgccg
     3961 cctttgcgcc gcatgccgga ggttcgcccc cgtccttcct atgtctgacc tgaatgccgt
     4021 ctgaatcaat atgaagatat acgtttcaga cggcatcccc ctgttgtgga aataccgtcc
     4081 gcccccccct gtccgattat tgcctgattg cggcggcgcg gacgagttct tcgaggctgt
     4141 cggcaagcat ttccgccgcg cgccggtagc cttgggcgga gaagtgtacg ccgtctttgg
     4201 cggcccatcc ttggttgagc cagtttttca tgctgcatat gccgcccatt gcgttttgcc
     4261 aagaccaaaa catcgtctgc ccctgacggg cgacgcgccg ctgcatctgt tggacttcgg
     4321 tcaggaggac ggggcgcgtg ccgcatacgc cgagcgtgtt tttcagggat tcgggcgcgc
     4381 cgatgatgag gatgccggcg gcgggcaggc tgtcgcggat ttggcggacg gtatccagcc
     4441 atttttgttc ggtatcggca atgtcgatgt tgttgttgaa ggcttcgttg gtgccgtagg
     4501 aaaggataac caaatcggcg ccggtttggg caaggtcgtt catacggtcg gcacgccatt
     4561 tcgaccactg ggtcaattgt gcgccgttga tgcccatcgc ggaaacggta atgccgccgg
     4621 cgggattttc gatgttgatg aagccgatgt cccacggcat ttcggtctgt atggccaggg
     4681 gcagtgccgc gcccgtatcc agtacctgcc agccgccgcc gttggcggag acggtgttgc
     4741 cgttgacggt cagggtttgt tcggcgagca gcggtttggc aaacagggaa acgcgctgtt
     4801 tgccggtttt gccgtcagac gcggtcaggg tcatgctgcc gccgctgccg gtttgggcga
     4861 ggatgccgcc gagggggaag tctgctgcgg cacttcggct gttgaggctt tgccagttgc
     4921 cgttatagcg gacggccgcc atgcgctgcc ctttgacgtt ggcggggtaa acccagccta
     4981 tgccgccgtc gccccatgtt ttttgcaggc gtttgcgcag ggcgtcggta aagaagtcgc
     5041 cggcggtatg cgagtcgccg atttgcagga tgcggaaggc ctcgccgctg ccttgtgcga
     5101 cggatcggag ttttttcatc caaggcgagg cggcggcgtt gccgtagtcg gtcagcagcc
     5161 cgtttgtatc ggtgtagggg gcggacgggg aaacggtaac ggtgtcgggg ctgacggagg
     5221 cgacgggcag ggcttctgcc tgcgtggcgg cgaacagggc ggaaaatgcg atgaagtgtt
     5281 tggggttcat ggctgtgtac ttgatggttg cgtactcggt tcaaaaacga ttttttccat
     5341 tattttttcc gccagcagtt tttgtccttc ggcggtaaag tgtatgccgt ccttactgcg
     5401 gtagcggacg ggtttgccgt tgacgttgac ggaatcggtg tagcggcctt tcccgccgct
     5461 cagtgtttgc gcggtgggaa tcaggatgat tttgcctttc aagtgttccg aaagcagttt
     5521 gtcgaggtag cgcatctgac cgtcgagctt gacttttttc atgtagggga tgccgagcca
     5581 gacgacttgg acgcggtgcg tgtgtgcggc ttcaaggatg cggtcgacgc gtttcaggta
     5641 ttcttgcgcc cattcgtcgg aagcgaattt gaggtagcgt ttgccgacgg ggaaatccca
     5701 cgggtcgttc gggccgagga agacggcgag tacgctgatt tcgggatgtt ttttcaaggt
     5761 ttcttcaatc gttttcggcc agtcgaagaa tgagggatag gaaagccccg tgctttgttt
     5821 gctgaggttg gcggattcga tgccgtattg ctgtttcagg cttttttgca cgaagggcgc
     5881 aacgccctgc atcagcgaat ctccggcgaa aaagactttg tcgccgctgc ggacggcggc
     5941 ggcttcggtg ccttgtttcc attctgttcc gccggccggc ggggcggctt cggatgcggc
     6001 ggcttcaggc ggcatatctg ccgaaccgcc gtcttgagcc gtcggcggcg tttcgccgga
     6061 caggaaggtt ttgatgccgt ctgaaagggc gtaggcgttt tcttgcaacg ccgctccgct
     6121 ccgccaccat ccgtaggcgg caagcggttc gagcgggctg ttgcggtggt aggtctgctg
     6181 ccagtaggcg ttgatggggt tttggctgaa ccacacggca atcagggcag acatcaatat
     6241 ggaagtgaaa agggaaagaa agtttttcat gtccaaaccc ttaaaaattg gcataaataa
     6301 agccgggtat ccccgaaggg gcgaggacga taatcagcag caggatgatg gaaatctgga
     6361 taaaccacag ccacatcggg attttttcca aacctttgac cgcgccgtcg aaagcgcgtt
     6421 gcaggtaagg gtagagcagc atgacggatg caaacgaggc aagcagcagc atatccgccc
     6481 gttgcggcgc gttccagccg ccggcattgg caaagagggc actgaaaact gcgcctgcat
     6541 cgtcggggtt tgcggtattg aagacgacga aggtcaggca gacaaaatgg aaggtgatga
     6601 gccacgagag cggcgcaagg tatttcagac ggcatagcgc gtcgcgtccg aaataccggt
     6661 cgcccgtgtt gagcagcgcc agtgccgtgc cgtgcagcgc gccccaaatg aggaagttcc
     6721 agccgtagcc gtgccagatg cctgagagta ccattgccgc catcaggttg agctgtgtcc
     6781 gcaaaaagcc ttttttgctg ccgcccaagg ggatgtagat gtagtcgcgt atccaggtgg
     6841 aaaggctgat gtgccatttg tcccaaaatg cgcggatatt cgcggcacga agcggtgcgg
     6901 agaagttttt gggcagccgg aagcccagca gcatcgccat accgataacc aaatcggaat
     6961 atccggaaaa gtctaaaaag agttggaagg tatagccgta cacgcctgcc aacacgcccc
     7021 agccgtcgaa ttgggcggga ttttcaaata cgggcgacac ccagttttcc gccagtatcc
     7081 ccgccagcca ccattttttg gcaatgccca gtaaaatcag ggaaacggcg agggcggggc
     7141 ggacgggcga acgcggtcgg cgggtgcgga tttgcgccag tgcacccgcc tgttcgccgt
     7201 ccgtgctttt gaatgcggcg gcgcggataa tcgggccgga ggtaacggtg gggaaaaaac
     7261 tcaggtgcag cagcagctcg tgccacccaa aacgcgcggc gtgcggggcg cggaagcagt
     7321 aaaccagata ggcgacagac tggaaggtat aataggaaag ccccagcggc atcaggatgt
     7381 cgattgcgcc gccttttccg gcatattgtg caatcagcgg acggaaaaaa tcgaaatatt
     7441 tgaaaaagcc caaaacggta atcgaggcgg caatgccgca ccccagccaa aagcggcgcg
     7501 tactttcgcg atcggaacgg agcagttcgc ccaaaaggta cacgcaggag gaataaagga
     7561 cgatgattgc cgcaaatacg gggctaatat ggtagagcca gcccataccg gcagccaaaa
     7621 gcagcaggtt ttggacggac gggtatttcg caaagcccca gtaaatcggc aggaagacga
     7681 taaaaaacag tgcgaactcg acagacagca gcggcataaa ccatccttgg gacaaaaaaa
     7741 taggcgttaa aacacggcaa agcccgttat tttaagcagg ggcggatatt ccttcaatca
     7801 aaatgccgtc tgaagggcgg gaggggcttc agacggcatc gctcggtcag gttcggatat
     7861 ggtggttcaa attcaggcag acccgcaaac ctgccgccgc cgtcagcata cccgcaacgc
     7921 cgatgcagga aatgcccgag tattgcgtaa cctaatgtcc cagcagtgcg ccgccgccta
     7981 taccgacgtt gtaaagctcc gaataaatcg agttggcaag gtctgcggca tcggaggcga
     8041 aatccaaaac tttggcaacc atccccaagc tgacgaccac aatcgccgtc ccccatacga
     8101 acaccaacgc gtaaatggcg gcaggaaaat gcgccaaagg cagcaaaagc cccgaagaga
     8161 gggcgataag cgacaccgcg cccgccaaaa atgcgcgcgg atgtttggca aaccatttgc
     8221 cgaacaggta agacgcggca aaacccgcca ggccgtacag ccccggaacc accgtaacct
     8281 gccgcgcgga gaaaccgccg atttggatga cgaagggctc gatgtagctg tatgcggtaa
     8341 agtgcgccgt aatcaccggc accgttaccg catacagcag catcagtttt ttccgtttca
     8401 aaagcagggg caggctgctt aaagaaccgg tattcacgct cggcaggcgc ggcaggcttt
     8461 tggcaagcac tgccatcacc gccgccgaac acaggccgat cagcagaaaa ctcgcctgcc
     8521 agcccaaata ttgtccgacc atacgcccga gcggaatccc cgccaccatc gccatcaccg
     8581 tccccgtgct gagcagcccc aatgcctgat tcccttttcc gacaagggcg atgcgtaccg
     8641 ccagcgaagc cgtaatggac cagaacaccg cgtgcgtcag cgcaatcccc acgcggcttg
     8701 cgagtaggat ttcaaaacgc cgcgaggcaa acgacaggat gtggctgaca gtaatacagc
     8761 gcaaacagga ccagcagcag gccgcgccgt tccataattg cgcgtcagca gcatcagcgg
     8821 cagcggcagc gaagtcagcg cgacaatcca cgcataaacc gtaatcatca cacccgtttc
     8881 ggtcgccgcc atgccgaagc ttcgtccgat gtcgctcaaa agcgcgatgg ggatgtattc
     8941 ggccgtgttg aaaataaatg cgccgattgc caaggcaacg atgctcaacc aaggtttgtg
     9001 cgaagcgttc atctcaattc cttatatgtc gcaccccgcc aaaagggggc gcattatggt
     9061 gaattaacaa aaaccggtac ggcgttgccc cgccttagct caaagggaac gattccctaa
     9121 ggtgatgaag caccaggcga accggttccg taccatttgt actgcctgcg gcccgccgcc
     9181 ttgtcctgat ttttgttaat tcactatact tttcttgtct gccggacggc aaaatccaaa
     9241 tcagggggaa gcagccgcct cccgttcgga ggaacactcg ggacgctgct ttgaaaatcg
     9301 ttccatatgt tgaaacattg ccgcctcccg attgggcaag gttgccatta tgtcgcaagc
     9361 atccgtgccg caccgtttcc gcgtttgccc aagttcacgc tccgacttac aataacgccc
     9421 gtcagattgc agtaccaacg aaaggaaaaa tcgtggaaac cctcctcctc gacatcgtcg
     9481 gcatgagctg cggcggctgc gtcaaaagcg ttacccggat attagaaagc gtaaaaggcg
     9541 tggcaagcgt cgaagtcagc cttgaaaaca aaagtgcgac cgtcggatac gaccccgcgc
     9601 aaaccgcagc ggaagcacta atcgaagccg tagaagacgg cggctatgat gcggcgttga
     9661 aataaagcgg caaaaatgcc gtctgaagcc tttgcgtttc cccgatgccg tctgaagcac
     9721 gctccgcgcc ttcgggcggc atttgattta gttgaacggg caggatgaat tggttaaact
     9781 tgcgggcaat agtgccttca gacaaacaaa atatgccgaa caaaaagaaa aaagccgcct
     9841 ttgcgcgctg caacggagaa cggtgtgccg ggcgtgtccg acatcaaaaa ttcatatgat
     9901 gatttgatgt acgaatccaa agcattttcc caaaccgcca tcaacgcctt ggaggcatgc
     9961 gcccgcatga tgggtttgaa gcctacggca gccgcaaaag ccaaggtctt ggagctgggc
    10021 tgttcgatgg gcagaaacat catcacgcag gcactttatt acccggatgc ggaatttgtc
    10081 ggtatcgact tgtccggcag gcaggttgcg cagggcaacg ccatcattga aaaaatgggc
    10141 ttggaaaatg tgcgtctgga agaaaaagat attttgaccg tcgatgaatc attcgggaag
    10201 ttcgactata tcatcgtcca cggcatttgg tcgcgggtgc ccgacgcagt taaagacaaa
    10261 atcttttcga tttgccggaa caacctgacc gaatacggca ttgcctatat ttcatacaat
    10321 gtttaccccg gttggaagcg gcaggagcag ttgcgcgata ttatgcagtt tgccggcagg
    10381 gatgcgcttg gagaaccctt ggaagcaagg acgcggaaag gcttggatgc gatcaaggcg
    10441 ctggcggaaa ttttggaaaa cgacaagggc ttgggcggcg gcaaacttcc ggcgattcaa
    10501 aaaatattga atcacaatac ttattatgtc gcacacgaat atatggaaat attcaacgac
    10561 ccgatttacg ttaacggttt cattgaatgg gcaaaccgcc accggctggc gtatatcggg
    10621 gataccgatt tgcatgtgtc ttttgtttct tggatggcgg agcatacgcg ggagcggatt
    10681 ctggcattgg cagggggtga ttatattgcc aaagaatttt acagcgatat tttatccgac
    10741 cgccaattcc accgttcgct tttatgccgt gaggaagtcg gggatactgt caggcgtgat
    10801 gagtcggttg ccgtcgaagt gatagaaagt ttgaatttcc gcccggcaag aggggaaaca
    10861 atcaactttg atgaaaacga taccctgctt tcgggcatac gcgatgtgat gaaaaccgga
    10921 gaggcgttta aaacggaaga tgttgcggaa aatcttgccc gccgatttcc cggtctggaa
    10981 tttgaccgca tgaaaatcaa ttcccagctt ttattgcaaa ccattctcgg gcgtttttct
    11041 gtttcatcag acaatgcggg caaaccattt tttgaagacc ataaaaccta tgtgccggcg
    11101 cgctttacaa actatgccgc cgcctttgtg gaacacggtg cgggagcgtt tgtccggcct
    11161 gccaaccgtt acaacgaaag cacgccctca ttcggatacg ggcatttgta cattatgcgg
    11221 caattgtcgc ggccgacgag caaacaggca ttgattgaaa cggttgccga aaacttgaac
    11281 atcgtcagcg ccacgcccga cggtttgaca ttccatccgc ctgccgaagt gtatgtggaa
    11341 gaaatattgg cagacttggc agacaggcat tttctcgttt cggcggattg acgggcaagt
    11401 cccgacggca atccgtccga tatattttca gacaggaaaa acacacactg cgaaaaacgg
    11461 tacacgacac atatgaacca gacatttacc ttgcccgata cgcgcccgta tccccaaaat
    11521 ccgattaaaa accacctgct gctcaatgcc taccagttgg cgcacaattc gtcacaggct
    11581 tcgcgcaaac tctcgtccgg gcagcttcaa accgaaatca gggggatgct tgagcaaaac
    11641 cactatatca acctttccct cgcgctgacg atgtcgcccg atgccggaac ttatgccgcg
    11701 ctgctttcca gtgtgaacgc ggtactcgat tgcgagaaag aaggcgaagt gcagtggttc
    11761 gccctgccgg tcgtgctggt gtccggctgc aagaaagaac gggcaatcga gatgaagctg
    11821 ccgacggagg cattgtttgc ctgcctgcaa aactatccgc acctgcgcgc gttgacgcaa
    11881 gagacacaat ggctgcctta tcttgtgcat tcttccgatt tgagtgcggt cgcgccggat
    11941 gagtggtggc gtgccaaaca aaataccgaa gcggcggcgc aacacttacg ccgtttcgcc
    12001 ccgcgccctt tgctgttgcc cgaagggcag tccgtccacg tcgtttacgt gctgggtttc
    12061 ggcagcggca aagttcaggc ggcattgggg cagaaccttc ttcaggcagg cttgccctta
    12121 atgcaggtat ggcaggaaaa tcttgcatcg gaaggcatta cgctgtttgc caatccgctt
    12181 tcccccgata ccccggcgcg cgcgctttca gacggcagcc acacgcgcca acgtatggcg
    12241 atggatgttt ttgcggcaaa cgcgatacgc gccgtccgga tgcagagtcc gcgcgtcggc
    12301 gtggtcgctg cggcaaaggc gggcggacag attctattcg gatttaatgc gaccgacggc
    12361 gcgtttgaag tcgtgccgca ggtgttttgc tggcagcttt cctttaccga taacattgcc
    12421 gtcatccagc agaatttcct cgacctgatg gcagaatgcc gtgtagaaca cgtttacctg
    12481 ctgcacaatc ccttgggcga acaggaaaac atcccgagct atgcggaagc gttgaaacgg
    12541 gaagggcgca atccgttttt cagcgcatag tgatttgtag tagtgatagg ctttctcatt
    12601 tctataataa atcaaaataa agagcacaac actttttcat tctgtgttgt gccttgagtg
    12661 aaacgaaagg atgaattata aatatgaaga ttgtaattgc accggattcg tttaaggaaa
    12721 gcttgacagc tcaacaggta gctgaagcaa taaaaagagg cttccaacaa tcgatagcag
    12781 atgtggaatg tctcctctgt cctgtcggtg atgggggaga aggcactgta gatgctatcc
    12841 gacattctct tgacctagaa gaaaaatgtc tccaagtgac aggacctttt ggacaaaaag
    12901 aagtcatgcg ctattttcaa aaagaacaac tagccctatt cgaagttgct gatttggttg
    12961 gtcttggaaa aatcccgcta gagaaacgaa atccattaca aatccaaact cgtggtattg
    13021 gagaattgat tcgccacctc attagtcaag aaattaaaga aatctatatt ggcgttggcg
    13081 gtacggccgg taatgatgga ggtattggga ttgctgctgg tttaggttat caattttatg
    13141 atgaggatgg aaatgcctta cccgcttgcg gtcaatcctt actaaaccca gcttctgttt
    13201 caacagaaaa tcgctatgaa attcctgaag atgttcacat tcgtatttta gcagatgttg
    13261 tgagtccctt atgtggacac caaggtgcga cctatacgtt tggcaagcaa aaagggctgg
    13321 attctactat gtttgaggcc gtagatcagg caatacaaga tttttatgaa aaagtccccc
    13381 ctgcaacatt aaaacttaaa ggagcaggag ctggtggagg catcgctggt ggtttgtgtg
    13441 cctttgctca ggcaagtatc gtatctggaa ttgacacctg cctggacttg attgactttg
    13501 ataagaaagt gtcagatgtt gacttggtta tcgttggtga aggaagacta gatcgtcaaa
    13561 gtttagcagg gaaagcgcct attggtgtag caaaaagaac ccctgtcgga gttcctgttg
    13621 tcgctatttg cggcagtttc gctgaagatt tgccttccct accatttgaa aatatccaag
    13681 ctgccttttc tattttggag aaaagtgaac ctttagaaga tagtttgaaa aatgccagtc
    13741 tttatttgga gcatacggct tctaatattg gacatttatt aaatatgcct aagatttagc
    13801 caagccattt attccagatg gatgttttgg caggttcttc cttatgcggt tacgattaac
    13861 cggccgatgc cgtctgaaag ggtttccgct tcaggcggca tcgcgttgga acagggatgg
    13921 gatatgaaaa aaccgaaaat cctttttgtc tgcctcggca acatctgccg ttcgccgatg
    13981 gcggaataca ttttgcgccg ccgtgccgcc gaagcgggca ttcccctcga aacggacagc
    14041 gcagggacat cgggctggca cgacggcgag gatatgcacc gtgaaacggc aaagatattg
    14101 aaaaaatacg gcattgacgc ttcaggcttt accagccgca aaatccgcca aagcgatgcg
    14161 acggcgtttg actacatcat cgctatggac ggcaagaatt tgtccgaatt ggaaaaaact
    14221 ttcggcaggc ggccggaaaa aatattcaag ctgaccgacc tgatacccga aagcggttac
    14281 gaccatgtcc ccgatccgtg gtacatgggt gattttgaag aaacatacag gcttgcggat
    14341 gcaggctgtc gggcattatt ggaaaagatt tccaaataaa gcaattgaat acaaacacaa
    14401 atataaaacc gcctcctgtc gtatcggtcg ttcagacggc ataaacaggg aatttatgaa
    14461 aacaaatttc aaacagaaaa ttatcgaaca ggcacgcaga gaggacttgc aggtaaccgc
    14521 tttgcgcgag caggtattag atattgtctt gcagcaaagc ggcgtgatta aagcctacaa
    14581 tgttttgtca cagatgcagc agcagagcga aggcgtgctt gcaccgccta ccgcctaccg
    14641 tgcccttgat ttttgggcgg atcagggcgt tttgcacaaa gtggcggcgg tcaacggcta
    14701 tattttgtgc agccacgcgc agcacgagtg caacgaccat tgccacgacc acgaagaagc
    14761 cgaagcgcac cacagcgcgt ttattttggt ctgcaccgaa tgcggcgcgg cggacgagca
    14821 aaccctcagc cacgagtggg cggcactgcg tgcaggcgtt gccgaaagcg gttttgcgct
    14881 gaaagaagaa cacgttgttt taactggaat ctgtaaaaaa tgtcagaagt gaaaaaaacc
    14941 aaagtccacc tgatttcagg ttttctggga acaggcaaaa ccaccgcgct caaaagcctg
    15001 atggcgcaaa aagacccgaa cgaaaaatgg gtcatcatcg tcaacgagtt cggcgaaatc
    15061 ggcattgacg gcgcggtatt gagcgacaac ggcatccctg tggcagaaat cgccggcggc
    15121 tgtttatgct gcaccgccgg cccgcaaatg ggcgtaaccg tgcagaaaat gctgcgcgac
    15181 gccaaacccg accgcctgat gattgaggca agcggactgg cgcacgccgc cagcgtcatc
    15241 gacgaattga aagccaaacc tctggacagt cttttggaaa tcggcgccgt ctttaccgtc
    15301 gtcgatccgc gccagttcat caatcccgat tacgcgcaac aggcgttgta taaagaccaa
    15361 atcggcattt gcgacgtatt ggccgccagc aaaaccgatt tatgcacccc cgaacagctt
    15421 gccgaatttc atgacaaagc ggcaaaactg ttcccaccca aagccaaagt ggtcgaagtc
    15481 caaaacgcac aactcgatat ccaatggctc gatattcccg tagttgaaaa atcacgctac
    15541 cgcctcaaag ccctgccgga caacaccatg ggcttccagt cgcaaggttt cacattcccc
    15601 gccggacgcg atttcgacgg cgaaaaattg accaacttct tcaatgattt gcccaaaatg
    15661 accgaaggac tcgtccgcgc caaaggcgtg ttccaagtat tgggaacgtg ggtgtggctc
    15721 aactgggtgg acgggcagtg gggcgcgaac caagtgtctt ggcggcgcga ttcgcgtttc
    15781 gagctgattg ccaaatcgtt tgacgcggat ttaatcgaac aaaaacttaa agacgcattg
    15841 gaatagcgtt cacgcgcttt cagacggcat atgaaatgaa aatgccgtct gaaggcttta
    15901 ggaggacggg atgaaaccca aattcaaaac cgttttaacc gcgctgcttt tggcggtttg
    15961 cctgccgtct atggcggcaa cccgcgtcct gatggaaacc gatatgggca atatccgttt
    16021 ggttttggac gaatccaaag cctccaaaac cgttgccaat ttcgtgcgct atgcccgaaa
    16081 aggcttttac gacaacacga ttttccaccg cgtcatcggc ggcttcgtca tccaaggcgg
    16141 cggattgacc gaggacttgg tgcaaaaggc aaccgataag gccgttgcca acgaatccgg
    16201 caacggcttg aaaaataccg tcggcaccat cgcaatggcg cggacggcag accccgattc
    16261 cgccgccgcc caattcttta tcaatctggc ggacaacggt tcgctcgact acaaaaacgg
    16321 acaatacggc tacaccgttt tcggcagggt agaaagcgga atggacaccg tttccaaaat
    16381 cgcccgcgtc aaaaccgcca cgcgcggcct ttatcaaaac gtacccatac agcccgtcaa
    16441 aatccgccgc gttgttgtcg agtaatataa cgggaaacct tcagaaagcc gaaacggaaa
    16501 aagcaggcgt ttggggttga aaagcgtctg cttcgggatt ttaaaatgcc gtctgaaacg
    16561 ccaaacgggc ttcagacggc atttttgacg gtggaggttt acgcgccgca ggttttcggc
    16621 ttgttcgcca gaatgttgat gactttgcgt tcggcttttt gcggctcgat tttgatttcg
    16681 ctctcatctt cttcgctgcc gtctgaaaag cgttcgggca ttttttcgct gtcaaaagcc
    16741 aaatcgccgc cgtgtttcag attttgaccg cgttccaatc cggcaaagtc aaagagttcg
    16801 gtatcggcaa ggtgggaagg gacgacgttt tgcagggcgg agaacatcga ttcgatacgg
    16861 ccggggaagc gtttgtccca atcgcgcagc atatcgccga tgacttggcg ttgtaggttg
    16921 ggctgcgagc cgcagaggtt gcacgggata atcgggaact gtttcaattc ggcgtatttg
    16981 attaagtctt tttctttcac atacgccagc gggcggatga cgatgtgttc gccgttgtcg
    17041 ctcaccagct tgggcggcat ggctttgagt ttgccgccgt aaaacatatt taaaaacatg
    17101 gtggcgagga tgtcgtcgcg gtggtgtccc aaggcgattt tggtgcagcc caattctttt
    17161 gcggtgcggt agaggatgcc gcgccgcagg cggctgcaca gcgaacaagt cgttttgcct
    17221 tcgtccaaca cgcgtttgac ggtggagtaa gtgtcttctt caacgatttt gtagggaacg
    17281 ccgatgcttt cgagataggt cggcaatact tcttcgggga agcccggctg tttttggtcg
    17341 agattgacgg cgaccagttc aaaatcaatc ggcgcgctgg cttggagccg gcgcaggatg
    17401 tctaacaggg cgtagctgtc tttgccgccg gagaggcaga ccatgatttt gtcgcccggc
    17461 tcgatcatgt tgaaatcgtt aatcgtgtcg ccgacggcgt ggcgcaggcg tttgctgagt
    17521 ttgttgtttt cgagttcttg cttggttttt ttggacatgg cggtggtttg aaaaattagg
    17581 gatgcggcat tgtaaccgaa aacaggggcg gcaatgccgt ctgaagggct tcagacggca
    17641 ttagagggct tattctgcat ttttggtttt aaagaagaga tgaaccgctt tgaagatacc
    17701 gccgtttggg acggtgagtt ctttttgcgc ggcggagaat ttaatcacgg caagggcggt
    17761 aaagttttcc gaatcttgaa cgctgtcgag tacgatgccg gcttcttcgc cgtccgccgt
    17821 cagcaggatg cccgcttcgg ctgccgaatt tcccgacaat accgccaagc cgcgtttgac
    17881 ctgcccccga tactgggcgc gcgcgatgat ttcctgtccc gggtagcagc cttttttgaa
    17941 gtgtacgccg ccgatgatgt gctggttgag catttgggcg acggcggttt ctttggtagc
    18001 cgcgcatatc cacggataac cgctgcggat ttcgtgcagc cgccacgcgt tttcggcggc
    18061 agcgtcataa gggggtaggg cggtttcggg ggcgatgtgc aaaatgcccc gatggtgcag
    18121 gatgacggaa cagatgccgt ctgagacgca ttcggaggta aaggcgagac tgggttcttg
    18181 cgcggcaagc ggttcggcgg atgctgccaa ttccgcgccg acggcgtaat cttcaaggat
    18241 ttcaaaaacg gctttggcgc gcaacacaaa catccgcagg cgtttgaccg ttgcttcaag
    18301 caaatcttgc gccataatca gcagcaaatc gtctccacgg ttgacgacaa tcatattggc
    18361 gatgacgcgg cctttgggcg tgttgtaagt cgcgtaacac gcctgcccgg cctgaagatt
    18421 attgatgtcg ttggaaagct gtccgtgcag gaaggtttgg cggtcttcgc cgctgacgcg
    18481 cgccacgccg aaaaagggca gtaaggtttt catcatttgc gtactctgaa aatataagga
    18541 aatctgttta tgcagttgcc gcgtctctct tcacggcggt tattttgatt tcgacgcgcc
    18601 actccggacg ggcgagccgc gcttccacgc aggcgcgggc gggcgttctg ccggcggcaa
    18661 cccaagcgtc ccacacgccg ttcatttccg catagtcgcc catatcacgc aggtagatga
    18721 ccgcatccaa aacgtgtgcc ttgtccgagc cgcattccgc cagccagcgg tcggtttggg
    18781 caagcacgtc ggcagtctgt tcggcagccg tttcaccgtt ttcgggaacc atgccggaga
    18841 ggaaaatcaa gccgcccgcg caaaccgctt cggaatagcg gggcgttgtg ccgaaatacc
    18901 ggatatccat atcggtttcc ttcgataaag gggatatatg gtaacattgc gcttgaccga
    18961 tttccatgtt ttgcatgacg aaaaatgagt aaacacactt atccgataac acctgccgta
    19021 cgcgttttgc gtgaaaacgg catcgaattt gaacctttta cctatgccta tgaggaacac
    19081 ggcggcacgg cgcagtttgc ccgactgttc ggcaaagacg aacacttggt cattaaaacc
    19141 attgttttgc aagatgaaaa cggtaagggg ccgattgtct tgatgcacgg cgacaaacag
    19201 atttcaaccc gcaatctggc gcggcatttg ggtgcgaaac acatcgaacc cgccgcgcct
    19261 gcgcaggcaa acaagtggac gggctatctg gtcggcggca caacgccgtt cggcatccgg
    19321 acaaaattgg atatttacgt cgaacagtcg gtgatggatt tggaaaccat ctatatcaat
    19381 ggcggaaaac gcgggttcat tatcggtatc cgccccgatg atttaaatat tttgaacccg
    19441 aaaacaatac aggcagcggt ttgaccggga agtataagga gcaatatgga caaagatttg
    19501 tatgccgtat tgggcgtgtc gccgcaggcg ggggcggacg aaatcaaacg cgcctaccgc
    19561 aagctggcga tgaaatacca tcccgaccgc aatccgggca atctgcaggc ggaagaaaag
    19621 ttcaaagaaa tccaacgggc ttacgatacg ctttccgacc tgtcaaaacg gacgcaatac
    19681 gacgcgtcct tcagacggca tgaggaacgc gggcggcagg aagaggcatt ccgccgcgaa
    19741 caggcgcgca gggagcagtt ttacggcgaa cagatgcgcc gcgaacaggc gttcagacgg
    19801 gagtttgaac ggcaggcttc acgttcgcgc catgcttacg aaccgtccgg cagcggaagc
    19861 gggcgcaact atatcctcgc cgcctacatc ctgttcggtt tgggtgcaat catacagttc
    19921 atgcccatag tcggcgtgat tctcgcctat atgaaacgga acagtttgga cagcattgtc
    19981 tatgccgcac ataccgaata cctgattaaa accttttggc gcacattttg gctttatctt
    20041 ttgggcgcgc tgactgcccg tttcggcatc ggcgtgctga ttattattgc aacgaccgtc
    20101 tggtatttct accgcatcat cgccggcttt atccgcttca acgacggcag ggcggttgcg
    20161 cccgagaaat ggatatagag catggcctac ctgttaatca gcatcgtgtt cagcgtgtcg
    20221 gtttccattt tgctgaaaat ggcaaggaag aaaaaaatcg acatcgcgca ggcggtcgcc
    20281 gtcaattatg tggtcgcggt catactgacc ctgtcggtat tgaagccgga tgtcggcaat
    20341 atcggtgcat ttttgccgac gtggccgctg tttgccgctt tgggcgtgct gctgccgtcc
    20401 gtatttgtca ttatgggcaa atccgtagaa tccgccggta tcgtcaaatc cgacgcggcg
    20461 cagcgtttgt cgctgttttt accgattgtt gccgctttga cgctgtttgg cgaaaaactc
    20521 agtgaaggca aactgatcgg actgtgcctc gcctttgccg cactgttctg cctgctttgg
    20581 aaaagcgaca gcggcaaaaa atcaggcagt gcgtggcggc aggcgttatt gctgctcggt
    20641 gtgtgggcgg gttacggcat tatcgacatc ctgttcaaac agattgccaa aagcggcacg
    20701 gcatttgcgg gcaacctgct ggttgcattt gcgctggcgg gtgtgctgat gtttgcctgc
    20761 ctgtttgcca aatcggtcag atggcgcgtt gagagcgtgg tcggcggcat actcttgggc
    20821 ggtttgaatt ttatgaacat cgtaacctac atcactgcgc accaaatgat gaaggacagt
    20881 cccacgctgg tttttgccgg tatgaatatc ggtgtgattg ttttagggac gctttcgggc
    20941 gcattgttct ttaaggaaaa aatcaacaca atcaatacgg caggcattgt actgtcatta
    21001 tgctccattg cctgcctgtt ctattggaca gaagttaagg cactgttcgg catataatc
//
LOCUS       DAXFZR010000037        20459 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000037 DAXFZR010000000
VERSION     DAXFZR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20459
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.037"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(1..388)
                     /locus_tag="ACFJAS_001133"
                     /pseudo
     CDS             complement(1..388)
                     /locus_tag="ACFJAS_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(523..774)
                     /locus_tag="ACFJAS_001134"
     CDS             complement(523..774)
                     /locus_tag="ACFJAS_001134"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3982403.1"
                     /translation="MNSSNVNKEIQGKKLSLWARRQDGSVKWFCGQPVTRNAKDDTVT
                     ADATGNDGKIDTKHLPSTCRDAASAGCIETPPTAFYKNT"
     gene            complement(787..1061)
                     /locus_tag="ACFJAS_001135"
                     /pseudo
     CDS             complement(787..1061)
                     /locus_tag="ACFJAS_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1088..1402)
                     /locus_tag="ACFJAS_001136"
                     /pseudo
     CDS             complement(1088..1402)
                     /locus_tag="ACFJAS_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            1406..1714
                     /locus_tag="ACFJAS_001137"
                     /pseudo
     CDS             1406..1714
                     /locus_tag="ACFJAS_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(1789..3843)
                     /gene="metG"
                     /locus_tag="ACFJAS_001138"
     CDS             complement(1789..3843)
                     /gene="metG"
                     /locus_tag="ACFJAS_001138"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC3982404.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELFNPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDTDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQAGFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(3959..5797)
                     /gene="glmS"
                     /locus_tag="ACFJAS_001139"
     CDS             complement(3959..5797)
                     /gene="glmS"
                     /locus_tag="ACFJAS_001139"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC3982405.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRIGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            6069..6596
                     /locus_tag="ACFJAS_001140"
     CDS             6069..6596
                     /locus_tag="ACFJAS_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982406.1"
                     /translation="MEMKQMLLAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGVIAVK
                     KEKGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRRYV
                     KTDAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQRQAAIEQLKRRFEAEFDEL
                     EKEIKCNGRTPALLF"
     gene            complement(6879..8204)
                     /locus_tag="ACFJAS_001141"
     CDS             complement(6879..8204)
                     /locus_tag="ACFJAS_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC3982407.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPIHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIY
                     GIPDDFISVPLPAGLRGGNALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSGGDGPVGALGTPLMGGYAGAIDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            8437..9257
                     /gene="efeU"
                     /locus_tag="ACFJAS_001142"
                     /pseudo
     CDS             8437..9257
                     /gene="efeU"
                     /locus_tag="ACFJAS_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            9254..10420
                     /gene="efeO"
                     /locus_tag="ACFJAS_001143"
     CDS             9254..10420
                     /gene="efeO"
                     /locus_tag="ACFJAS_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC3982408.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
     gene            10727..11990
                     /gene="efeB"
                     /locus_tag="ACFJAS_001144"
                     /pseudo
     CDS             10727..11990
                     /gene="efeB"
                     /locus_tag="ACFJAS_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
     gene            complement(12062..12391)
                     /locus_tag="ACFJAS_001145"
     CDS             complement(12062..12391)
                     /locus_tag="ACFJAS_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC3982409.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(12466..13836)
                     /gene="glmU"
                     /locus_tag="ACFJAS_001146"
     CDS             complement(12466..13836)
                     /gene="glmU"
                     /locus_tag="ACFJAS_001146"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC3982410.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(13891..14163)
                     /locus_tag="ACFJAS_001147"
     CDS             complement(13891..14163)
                     /locus_tag="ACFJAS_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC3982411.1"
                     /translation="MRKTFLILTVSAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(14156..14872)
                     /locus_tag="ACFJAS_001148"
     CDS             complement(14156..14872)
                     /locus_tag="ACFJAS_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC3982412.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHTPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTPPPENTKECYA"
     gene            14931..15932
                     /locus_tag="ACFJAS_001149"
     CDS             14931..15932
                     /locus_tag="ACFJAS_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3982413.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSLEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            16026..16874
                     /locus_tag="ACFJAS_001150"
     CDS             16026..16874
                     /locus_tag="ACFJAS_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC3982414.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            16901..17383
                     /locus_tag="ACFJAS_001151"
     CDS             16901..17383
                     /locus_tag="ACFJAS_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC3982415.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDAV
                     "
     gene            complement(17467..19035)
                     /gene="msrAB"
                     /locus_tag="ACFJAS_001152"
     CDS             complement(17467..19035)
                     /gene="msrAB"
                     /locus_tag="ACFJAS_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC3982416.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGTATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            19181..20434
                     /gene="ftsY"
                     /locus_tag="ACFJAS_001153"
     CDS             19181..20434
                     /gene="ftsY"
                     /locus_tag="ACFJAS_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC3982417.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         4166 a         5223 c         6264 g         4806 t
ORIGIN      
        1 agctggcatc aaagttatcg cggcaggttg acggcaggtg cttggtttcg attttggtgc
       61 cggtggtgtc ggcggtgacg gcgtcggtgc cggctttggc ggcgtcgtcg cgcttaaccg
      121 gctgtccgca gaaccatttt accgaaccgt cttcacgctt ggcccacagg gagagtcttt
      181 tgcctttgat ttcattgttt acgccgcttg gtttcatttg ggcggtaacg acgccttttg
      241 cgaccgtaac gctttgaaca tatttgcctt tgatgtcgga ggggggggat gccacgccgg
      301 cagaagtgtt gttttccggc cattcgccgt gattcaggta atactcggta acggctgatt
      361 tttgaccttc ggccaaaagg atggcttcgt cattcccgcg caggcgggaa tccagccccg
      421 tcggtacgga aacttatcgg ataaaacggt ttctttagat tttacgttcc ggattcccac
      481 tttcgcggga atgacgggat gtaggttcgt gggaatgacg gtttaggtat ttttatagaa
      541 agccgtaggt ggtgtttcta tgcaaccggc agatgctgcg tcgcggcagg ttgacggcag
      601 gtgcttggtg tcgattttgc cgtcgttgcc ggtggcgtcg gcggtgacgg tgtcgtcttt
      661 ggcgttgcgc gtaaccggct gtccgcagaa ccattttacc gaaccgtctt gacgcctggc
      721 ccacagggag agttttttgc cttggatttc tttgtttacg ttgcttgaat tcattgtggc
      781 ggtgacgtcg tctttggcgg cgttggcgtt gtcgcgctta accggctgtc cgcagaacca
      841 ttttaccgaa ccgtcttgac gcctggccca cagggagagt tttttgcctt tgatttcttt
      901 gtttacgccg gttgaagcca tttgggcggt aacgacgccg tttgtgactt caactttctg
      961 aacatatttg cctttgattg ttgaagcgga tgccacgccg gcagagccgt tgtctttggg
     1021 ccattcgccg ttttcaggta atattcggtg agggctgatt tatttgaaat ttaaggccta
     1081 atttgcctta gctagcagag aacgggtcac ggcaggttga cggcaggtgc ttggtttcga
     1141 ttttgttggc gtcggtgacg gcgtcgtcgc cgcgcttaac cggctgtccg cagaaccatt
     1201 ttaccgaacc ggcttcacgc ttggcccaca gggagagttt tttgtttttg atttcattgt
     1261 ttacgccgct tgaaagcatt ttggcggtaa cgacgccttt ttcgaccgta acgctttcaa
     1321 catatttgcc tttgatgtcg gaggcggatg ccacgccggc agcgccgttg tttgcgggcc
     1381 atttgccgtt attcgggtaa tgtccggcga aaacgaattg cggcagatga agccgggtgc
     1441 ggttttgatc aattgcgggc gcggcgggct ggcggatgaa aacgcgctgc ttgccgcact
     1501 caaatacggg cagatcggcg gggcgggtgt cgatgttttg acggaggaac cgccgagggg
     1561 cggcaatccc ttgctgaatg cacgattacc caatctgatt gttacgccgc ataccgcgtg
     1621 ggcgagccgc gaggctttgg atcggttgtt tgaaatattg ttggcgaaca ttcacgcctt
     1681 tgtgaaaggc gaggcgcaaa accgcgtggt ttgaacctgt cgggattgcg gaaaaaaacg
     1741 ccgtctgaac gcccaagggg ttcagacggc gttttttgag atttctgcct aaccgacttt
     1801 gtcgcccggc tgagcgcccg catccacgtc aagcagcttc agtttgccgt ctgccgtggc
     1861 ggcggagagg atcatacctt cggatacgcc gaattttgcc attttgcgtg gggcgaagtt
     1921 ggcgactgcg atgaccatgc gaccgttcaa ttcggcagga tttgggtaag acgcggcgat
     1981 gccggagaag atgatgcgtt tttcaaaacc gaaatcgagg tcgaatttca aaagtttggt
     2041 gctgccttcg acggcttcgc agttcaatac tttggcgacg cgcatatcga ttttcataaa
     2101 gtcgtcgaag cccgcctgtt cggcgacttt ttcgtattta ccctcttcaa cgggcgcgga
     2161 tgccgtctga atgctttgtt tgttggcttc gatcaaatcg tccacttgtt tttgctccac
     2221 tcgttgcatt aaatgttcgt atttgttgat ggcgtgttcg cctagggttt cgcgcgtgtt
     2281 cttccaggta atcgcgtcca gattgaggaa acgcgcggcg ttggcggcgg tttgcggcaa
     2341 cacgggggcg aggtaggcgg tcagcatggt gaaggcgttg atgagttcgc tgcatacttc
     2401 gtgcaggcgt tcgtcttgac cttcttgttt ggcgagctcc cacggtttgt tggcatcgac
     2461 gtattcgttg acaatgtctg ccaatgccat gatgtcgcgc agggcgcggg cgtattcgcg
     2521 gttttcgtat tgctcggcta tggtgtcgct ttcggcggcg agttttgcca gcaatgcgct
     2581 gcctgaaacg tctttcagac gaccttcaaa gcgtttggcg atgaaacctg aagcgcgggc
     2641 ggcgatgttg acgtatttgc cgacgaggtc gctgttgacg cgggaaataa agtcttgcag
     2701 gttcaaatcg gtgtcttcga ttttgctgtt gagcttggcg gcgatgtagt agcgcatcca
     2761 ctcggggttc aggccttgtt ccaaatagga tttggcggtg atgaaggtgc cgcgcgattt
     2821 ggacattttt tgtccgtcca cggtcaaaaa gccgtgtgcg tacacgccgg tcggggcgcg
     2881 gtggccggag aaatgcagca tggccggcca gaacagggcg tggaaataga ggatgtcttt
     2941 gccgatgaag tggtacatct cggtttggct gtcggctttg aagtattcgt cgaaatcgat
     3001 gccgatgcgg tcgcacaggt ttttaaacga cgccatatag ccgacgggcg cgtccagcca
     3061 aacgtagaag tatttgcccg gcgcgtcggg gatttcaaaa ccgaaatacg gcgcgtcgcg
     3121 ggaaatgtcc cagtcggaca gggtggtttc ttcgccttcg cccagccatt ccttcatttt
     3181 gttgagggct tcgggttgca gatggggctt gccgtcgtgc gggttgttgc cggaagtcca
     3241 tgctttgagg aagtcggcgc attcgcccag tttgaagaag aagtgttcgg attcgcgcaa
     3301 ttcgggtttc gcgccggaaa cggcggaata cgggttgaac agttcggtcg gggaataggt
     3361 cgtgccgcag acttcgcagt tgtcgccgta ttggtcttgg gcgtggcatt tcgggcattc
     3421 gcctttgacg aagcggtcgg gcaggaacat ttgtttttca gggtcgaaaa gctgctcgat
     3481 gacgcggctt tcgattttgc cgttggcttt cagcgcgcgg taaatgtctt gggaaaactg
     3541 tttgttttca gtggaatggg tgctgtaata attgtcgtag ccgatgaaaa agccggtaaa
     3601 gtcggcaagg tgctctttgc gcactttggc aatcatgtct tcgggcgcga tgccttgttt
     3661 ttgcgcggca agcataacgg gcgtgccgtg ggtgtcgtcg gcgcagcagt agtagcattc
     3721 gtggccgcgc agtttttgaa agcgcaccca aacgtcggtt tggatgtgtt cgaccatgtg
     3781 gccgaggtgg atgctgccgt tggcataggg cagggcggag gtaactaaga ttttgcgtgt
     3841 catattgtgc tttgcaaaca atgggtaaag gcggattata ccgcaaatca aacggggaaa
     3901 tgccgtctga agcctgaaaa atcgggcttc agacggcatt tttgccaacc ggcgggattt
     3961 attcgacggt tacggatttc gccaggttgc gcggcttgtc cacatccgtg ccgcgcgcga
     4021 gggcggtgtg gtaggcgagg agctgcacgg ggacggtatg cacgacgggg gacagttcgc
     4081 cgacgtggcg aggtgcgcgg ataacgtgca cgccttcggt ggcattaaaa ttgctgtcga
     4141 ggtcggcaaa gacgaaaagt tcgccgccgc gcgcgccgac ttcctgcata ttggctttga
     4201 ctttgtccaa caggctgtcg ttgggcgcga tgacgacgac gggcatgttt tcgtccacca
     4261 aggcaagcgg cccgtgtttc agttcgccgg caggataggc ttcggcgtgg atgtaggtga
     4321 tttcctttag cttcaatgca ccttcaaggg caatcgggta atggatgccg cgccccaaaa
     4381 acagcgcgct ggttttcttg gcaaactgtt gcgcccaagc ggcaatttgc ggttcgaggt
     4441 tcaaagcgtg ctgcacgctg ccgggaagct ggcggagttc ttcggtgtaa cgtgcttcat
     4501 cttcgccgga taccacgccg cgcactttcg ccagcgttac cgccaaaccg aacagcacga
     4561 ccagttgcgt ggtaaatgct ttagtcgagg cgacgccgat ttccgcaccg gcacgggtat
     4621 aaagcacaag gctgctttcg cgcggcaggg cggattccat cacgttgcag acggagaggc
     4681 tgtggcggtg tcccaaagat ttggcgtatt tcaaagcctc catcgtgtcc agcgtttcgc
     4741 cggattggga aatggtaatg accagttggt cggggtcggc aatcacgctg cggtaccggt
     4801 attcgcttgc gatttcgacg tcggccggga ttttggcgat ggattccaac caatatttgg
     4861 cggtcagcgc ggcgtaatag gacgtgccgc aggcaaggat tttgacgctg cggatgcttt
     4921 caaacacgct tttggcattt ttgccgaagt tttcggggat gaatccgccg tcgaggaaaa
     4981 cttccgccgt gtccgcaatc gcgcgcggct gctcgtggat ttctttttgc ataaagtggc
     5041 tgtgcggacc cggttccaaa gaggcgggcg agagttcgga taccttgact ttgcgttcgg
     5101 caggcaggcc gtttttatcg gtcagccgtt tgatgccgtc tgaagccagc agcgcgatgt
     5161 cgccgtcttc gaggtacgcc acgcggcgcg taaaggcgat gacggcggat acgtccgaag
     5221 cgataaaggt ttcatcgtcg cccaaagcga ccaaaagcgg gcagcccata cgcgccacaa
     5281 ctaattcatc aggcttgtct tgggcaataa ccgcgatggc gtatgcgccg tggaaacgtt
     5341 tgaccgcttc ctgcacggct tcaaacagcc tgccgccgtt ttgcgcgtat tcgtggttga
     5401 tgctgtgtgc gatgacttcg gtatccgttt gcgattcaaa ccggtatccc aaaccttcca
     5461 agcgtttgcg ttcgctttca aagttttcga tgatgccgtt gtgtacgacc gcaatcatac
     5521 cgccgctgat gtgcgggtgg gcattcggct cggtaacgcc gccgtgtgtc gcccagcgcg
     5581 tatgcccgat gccgatgccg ccgctgatgc ctttttcgcg tgccgcgtcc tccataagct
     5641 gcacgcgtcc gatgcggcgc acgcgtttga ttttgccgtc ggtgttgacg gcaatgcctg
     5701 atgagtcgta accccggtat tcgaggcgtt tgaggccgtc ggtcagaaaa tcgacgacgt
     5761 tgtgatgggc gcggatggcg ccgacgatac cgcacataaa ctgttcctta gtatccggtt
     5821 gaaaaaaaag atgcggacgg cttccgtacc gcaccttccg tttcggatta taaaccgcct
     5881 cccgcgccgg aaaacagcaa aatgccgtct gaaggcttgg gcttgctcaa aaaagggagg
     5941 gatttccccg cctgaccggg atgggcgttc agacggcatt gcctgccgcc ggtttatagt
     6001 ttttgcaaat caatattaac aagctgaaaa aaaacaatat acccgcccgg tcttaatgtt
     6061 aacggagtat ggaaatgaaa caaatgcttt tggccgtcgg cgtggcggcg gtgttggcgg
     6121 gctgcggcaa ggatgccggc ggttacgagg gttattggcg cgaaaagtcg gacaaaaaag
     6181 agggcgtgat tgccgtcaaa aaagaaaaag gcaattactt ccttaataaa atcaacgtgt
     6241 ttacaggcaa ggaggagtct ttgcttttgt ctgaaaaaga cggcgcgctt tcgataaaca
     6301 cggggatagg ggaaatcccg atcaaacttt ccgacgacgg gaaagagctg tatgtcgaac
     6361 gcaggcggta tgtgaaaacc gatgcggcga tgaaggacaa aatcatcgcc caccagaaaa
     6421 agtgcggaca aacggcacag gcatacctcg acgcgcgaaa tgcgttgccg tcaaaccaaa
     6481 cgtatcagca gcgtcaggcg gcgatcgagc aattgaaacg gcggtttgaa gccgagtttg
     6541 acgaattgga aaaagaaatc aaatgcaacg gcagaacccc ggcattgttg ttttagcgta
     6601 ggacaaccgg gaggatgccg ccgtccgaat cggacgcgtg gtttccatac cggtacgggc
     6661 gggcgggaat gtccgccttt ttttgttcgg atgcgtttga atacccgtcc gattctgtcc
     6721 gtttgcaagg gggatttccg ttcgggcgga aatccattcc gtcaaataag aaaggcgttt
     6781 tgtccgaccg gccggcttgt gcctgcggta tcgcaaaact tcgccttaat gcgcccgatt
     6841 ggctagggac gggcttcgga cggcattgtt ttgccggttc acgggcggta ttcgggcttc
     6901 atgccgttgg gcaggagctg ccagacgtat cccgtggttt tctgtttgcc ggcaagttcg
     6961 ccggcttcgt cgccgtaacc ccaaaaataa tccacgcgca ccgcgccttt gatcgcgctg
     7021 cctgtatcct gcgccataat caggcggttg agggctttgc gggtaaccgg atgggcggtg
     7081 gcgacaaata agggtgcgcc caaggtaatg tagtgccggt cgattgcgcc ggcgtacccc
     7141 cccatcagcg gcgtgcccag tgcgccgacc gggccgtcgc cgccgcttcc ggcgagttcc
     7201 ctgaaaaaga tatagctggg gttttgcccc aaaacttcgg cgaggcgttg cgggttttgc
     7261 cgcatatagg ctttgatgcc ctgcatcgag gtctgcccga gcttgaggta gcctttgtcc
     7321 gccatatagc gtccgatgga aacatacgga tgttcgtttt tgtcggcgta tccgatgcgg
     7381 atgtatttgc cggacggggt tttcaggcgg cccgagcctt ggatgtgcat gaaaaaaagt
     7441 tcgacggggt cttcggcgta accgagtatc ggggctttgc cgtcaagcgc gccgccgttg
     7501 atttggtttc gcgtgtggta ggggaggaag cggcttcctt caaacctgcc cttgattgcc
     7561 gttgtgcgcg cggtaatggg gaatcgggaa aggtcggcgg tatgcgtgcc gccggcattg
     7621 tcgattgtgc cgctgttttt tcccgtctgc ctgatgcgga caagggcgtt cccgccccgc
     7681 aaaccggcag gcagcgggac ggagataaaa tcgtcgggaa taccgtaaat cgggaagcgg
     7741 gcccgttccg tccgcctgcc gtcgcccttc agcaccggct cgtaatagcc ggtaaccgta
     7801 cctgcaaggc ttccgttgcc tgcaacctgc cacggcgtga aatagcgttc aaaaaactgt
     7861 tttgcctgaa aggaatggat gggggtttga aaggcttggg cgcacacatc ctgccagcct
     7921 tggcggtttt tcaaattggc gcagccgagg cggaaggatt gcaggctttt ggcaaaatcc
     7981 tgcgccgccc agtggggcat ggacaggtgc ggcacaacgg tatagacggc cccgccgccg
     8041 gcaaccgtcg ttccggcggg gtcggggatg ccggccggcc ggtccgggcc gttgatgacg
     8101 gatgtgtcgg gttgcggaaa ggtttggatg ctcctgcttt ggcaggcggc gaggatggcg
     8161 gcggcgatgc cgtacagggc ggagcggagc aggtgttttt tcataatgga caatgttgtc
     8221 gtcagtaata aaaaaagatg gtttcgggcg gcgttgcggt agccggggag aggggatttt
     8281 aacacagggc gcagccgcag cctgcggaac tttccgccac gcggcaatgc agataaaaat
     8341 aacttgcatt tgtatttgca agtaatgaaa atattccgat aatatattat taatcatcct
     8401 tgttcattcg cgtttacgct ggtcgctttt ttaattatgt tgcgcgaggg tatcgaagcc
     8461 gcgctcatcg tcggcatcgt tgccggtttt ttgaaacagt ccggacattc aaaaactgat
     8521 gcctaaggtc tggttcgggg tcgtccttgc ttctttgatg tgtttgggca tcggatacgg
     8581 catccattcg gcaacgggcg agattccccg gaagcagcag gagttcgtcg tcggcattat
     8641 cggtttggtt gccgtcgcga tgctgacttg tgtggtttta tggatgaaaa aggcggcgcg
     8701 ttcgatgaag cggcagcttc gggattctgt acaggcggct ttgaaccgtg gcagcggtca
     8761 aggatgggcc ttggtcggta tggcgtttct tgccgtggcg cgcgaaggtt tggagagcgt
     8821 tttttttcct gcttgccgta ttcaaacaga gcccgacgtg gcagatgccg gccggcgcgg
     8881 tagcgggggt tttggctgcc gccgtgattg gcgcgttgat ttatcagggc gggatgcgcc
     8941 tgaatctggc gaagtttttc cgttggacgg gggcgtttct gattatcgtt gccgccggtc
     9001 tgcttgccgg ctcgctgcgt gcgctgcatg aggcaggcat ttggaacgcg cttcaggaca
     9061 ttgtgttcga cccatcaaaa tatttgcacg aagacagtcc gttgggtgtg ctgctcggcg
     9121 gatttttcgg ctataccgac catccggcgc agggcggggc cttggtttgg ctgctgtacc
     9181 ttattcccgt catgatttgg tttttgcgcg gcagcaggcc gtctgaaact ttaacccgta
     9241 aagaggaatt gaaatgagaa aattcaattt gaccgcattg tccgtgatgc ttgccttggg
     9301 tttgaccgcg tgccagccgc cggaggcgga gaaagccgcg ccggccgcgt ccggtgagac
     9361 ccaatccgcc aacgaaggcg gttcggtcgg tatcgccgtc aacgacaatg cctgcgaacc
     9421 gatgaatctg accgtgccga gcggacaggt tgtgttcaat attaaaaaca acagcggccg
     9481 caagctcgaa tgggaaatcc tgaagggcgt gatggtggtg gacgaacgcg aaaatatcgc
     9541 cccggggctt tccgacaaaa tgaccgtaac cctgctgccg ggggaatacg aaatgacctg
     9601 cggccttttg accaatccgc gcggcaagct ggtggtagcc gacagcggct ttaaagacac
     9661 cgccaacgaa gcggatttgg aaaaactgcc ccaaccgctc gccgactata aagcctacgt
     9721 tcaaggcgag gttaaagagc tggcggcgaa aaccaaaacc tttaccgaag ccgtcaaagc
     9781 aggcgacatt gaaaaggcga aatccctgtt tgccgacacc cgcgtccatt acgaacgcat
     9841 cgaaccgatt gccgagcttt tcagcgaact cgaccccgtc atcgatgcgc gtgaagacga
     9901 cttcaaagac ggcgcgaaag atgccgggtt taccggcttc caccgtatcg aacacgccct
     9961 ttgggtggaa aaagacgtat ccggagtgaa ggaaaccgcg gccaaactga tgaccgatgt
    10021 cgaagccctg caaaaagaaa tcgacgcatt ggcgttccct ccgggcaaag tggtcggcgg
    10081 cgcgtccgaa ctgattgaag aagcggcggg cagtaaaatc agcggcgaag aagaccgtta
    10141 cagccacacc gatttgagcg acttccaagc taatgcggac ggatctaaaa aaatcgtcga
    10201 tttgttccgt ccgttgattg aggccaaaaa caaagccttg ttggaaaaaa ccgataccaa
    10261 cttcaaacag gtcaacgaaa ttctggcgaa ataccgcacc aaagacggtt ttgaaaccta
    10321 cgacaagctg agcgaagccg accgcaaagc attacaggct cctattaacg cgcttgccga
    10381 agaccttgcc caacttcgcg gcatactcgg cttgaaataa gccgcaagcg ttcagacggc
    10441 atttggcggc agataccgtc tgaagtttta tagcggatta acaaaaaccg gtacgggttg
    10501 ccccgccccg gctcaaaggg aacggttccc taaggcgccc aagcaccggg cggatcggtt
    10561 ccgtaccatt tgtactgcct gcggcccgcc gccttgtcct gattttcgtt aatccgctat
    10621 atccgccata tgttgcaggg cgggttttca gaccgccgct atcggttaat ggaaaaacgg
    10681 cgtgcaggga tgcccaccct gctgcacgga tattgaagga aacgccatga gtcaaaacca
    10741 acccgcacaa ccgaccaaac gcaatctgtt caaaaccgcc cttgccgtcg gcgcaatcgg
    10801 cgcaatcgga ggttatttcg gcggcaaaaa acagggcgaa accgccgaac gcaccgccga
    10861 aagccaacac tcgccccaag cctatccctg ctacggcgaa catcaggcag gtatcgttac
    10921 gccgcagcag gcgttttcca ttatgtgcgc cttcgacgta accgcgcaaa gtgccaagca
    10981 gctggaaaac ctgttccgca cactgaccgc ccgcatcgag tttctcaccc aaggcggaga
    11041 ataccaagac ggcgacgaca aactcgtcag ccggcagcgg cattttgggc aaagccttca
    11101 acccagacgg attgaccgtt accgtggggg tgggcagcag cctgtttgac ggccggttcg
    11161 gactcaaaga caaaaaaacg gttcatttgc aggaaatgcg cgacttcccc aacgataagc
    11221 tgcaaaaaag ctggtgcgac ggcgatttga gcctgcaaat ctgcgccttc acccccgaaa
    11281 cctgccaagc cgccctgcgc gacatcatca aacacaccgc ccaaaccgcc gtcatccgct
    11341 ggagtatcga cgggtggcag cctaaatccg aacccggcgc gatggcggcg cgcaacctgt
    11401 tgggcttccg agacggcacg ggcaacccca aggtttccga tcccaaaacc gccgacgagg
    11461 ttttatggac gggcgtggcc gccaacagcc tcgacgaacc ggagtgggcg aaaaacggca
    11521 gctatcaggc agtccgcctt atccgccgct ttgtcgagtt ttgggacagg acgccgcttc
    11581 aagagcaaac cgacattttc gggcggcgaa aatacagcgg ggcgccgatg gacggcaaaa
    11641 aagaagccga ccaaccggat ttcgccaaag accccgaggg taataccacg cccaaagaca
    11701 gccatatgcg cctggcgaat ccgcgcgatc ccgaattcct caaaaaacac cgcctcttcc
    11761 gccgcgccta cagctattct cgcggacccg cctcaagcgg acagcttgat gtcgggctgg
    11821 tgttcgtctg ctatcaggca aatcttgccg acggtttcat cttcgtgcaa aacctcctca
    11881 acggcgaacc gctggaagaa tacatcagcc ccttcggcgg cggctatttc ttcgtcttgc
    11941 ccggcgtggg aaaaggcgga ttcttgggac aagggctgcc gggcgtataa acctgcctga
    12001 cggacaaatg ccgtccgaac cttgccaaac gggttcggac gggattttac tgtttggacg
    12061 gtcaggcttt tttgacgaat tcggatttta aattcatcgc gctgccgtcg attttgcagc
    12121 cgatgttgtg atcgccttcc tgcaggcgta tgcctttgac cttcgtacct tgtttgatcg
    12181 tcatcgaact gccttttacc ttgaggtctt tgatgaggat gacggtgtcg ccgttttgca
    12241 gcgccgcgcc gttggcatcg cgcacttgag ccgcaaggtc ggcggcggat tcggtttcat
    12301 tccattcgtg ggcgcattcg gggcagatgt attgtccgcc gtcttcatag gtgtattcgg
    12361 aggcgcattg cgggcaaggg ggtaatgaca tggtttgccg tccttatcgg atgtttgttt
    12421 tgggatgccg tctgaaacct gaaaccggct tcagacggca cggctttatt gtttgtcttt
    12481 ttccggcctt acccagcctt cgatgacggt ttggcgggcg cgggcgaggg cgagtttgtt
    12541 gtcttcgata ttgcgggtaa tcgcgctgcc tgcgcctgtg gttactttgt tgcccagtgt
    12601 tacaggggca accagtacgc agttcgaacc gatgcgcact tcgtcgccga tgacggtttt
    12661 gtgtttgtgc acgccgtcgt agttggcaat aatcgtgccc gcgccgaagt tggttttgct
    12721 gccgacttcg gcatcgccta tgtaggtgag gtggttggct ttggtgcctt tgccgatggc
    12781 ggcgtttttg atttcgacga agttgccgac gtgtacattg tcagccagtt tggcttgcgg
    12841 acgcaaacgg gcgtacgggc cgattcggtt gttttcgccg acttcgcagc cttcgaggtg
    12901 ggagaagggg gcgattttgc tgtttgcgcc gattttggcg tttttgatga cgcagtttgc
    12961 gccgatttcg acgttgtcgc cgagttcgac ttcaccttcg atgacgacgt tgacgtcaat
    13021 cacgacatcc tgtccgtgtt tcagacggcc ccgcaaatcg aaacgcgccg gatcgcgcag
    13081 ggtcacgcct gctttgagca attcttgcgc ctgttcggtt tggaagatgc gttcgagttc
    13141 ggcgagctgg cgtttattgt tcacgccggc ggcgaggtgg gaggcgcgca ctcggacggg
    13201 gcggactttg atgccgtcgg caacggcttt ggcgatgagg tcggttaaat aatattcgcc
    13261 ttgggcattg ttgctggaaa ggctgttcag ccagttttcc agtttggcgt tgggcaggac
    13321 gaggatgcct gtgttgattt cgcggatggt tttttgggtg gcacttgcgt ctttttcttc
    13381 gacaatggcg gttacgctgc cgctgccgtc gcggatgatg cgccccaagc ctgtcgggtc
    13441 ggcgggcacg tcggtcaaca gcccgacttc gttgcctgcg gcttcgagca gggtttcgag
    13501 ggtttcaacg tcaattaaag gaacgtcgcc gtacaacacc agcgtgcggc cttcggaggc
    13561 aaggtggggc agggcggttt tgacggcgtg gccggtgccg agttgttcgg tttgttcgac
    13621 ccagacggca tcgcgtttga cagtgtctaa aacttgctct ttgccgtgtc cgacgacgac
    13681 gcagatgttt tgcggattca gcgcggcggc ggtgtcgata acgcgctcga ccatgggctt
    13741 gccgccgatg cagtgcagca ctttgggcat ttgggaatac atgcgcgtgc ctttgccggc
    13801 ggcgaggatg acggtgttta aagtgttttg cggcatagcg ttttcctgtg ccatgccgtc
    13861 tgaagcggtt tcagacggca tagggtaggt ttatcggttt tgaaactttg gtttttgcca
    13921 gtgttggcga tgctcttcgt cggcgttgtt gccggtttgg tcgggcagca cggcgcggcg
    13981 ttcgggacgg tattggttgt agttcatatt tttcgagtag ctgccgtctt ggtaataaac
    14041 gggcgtgccg gcgggatatt tttgacggac ggcggttttg ccgttgccgt cttgataagt
    14101 ttcccacgcg cagcccgaca aaagggcggc ggagacggtc aggataagga aggttttacg
    14161 catagcattc tttcgtattt tcgggggggg gggtgcgggt attgtaatga ttttggcggc
    14221 gttctgacaa agtttctgca taccgtgcca gttgcgccat atcgcttacg gaggcatcga
    14281 taaagggcgg cgtgtgggat tttgcaccga accggacggt tttcatgccc aatgcttttg
    14341 cctgatggag gttgtccgcg ctgtcgtcca ccataatgca gcattcgggc ggtacgtcca
    14401 acaggcggca gacattgaga tacgcttgcg gattgggttt gtacagcagt ccgaaatcat
    14461 ccgtgccgaa cagtgcgtcg aaacagtttt ccaaaccgag tgcgccggca acggcacgga
    14521 cgtaaaacga cgggccgttg gaaaaaaccg ccttgcgccc gttcagacgg catagggtgt
    14581 tttccgtatc cgccatgccg tgcagcctgg tcaggattgc atcgatcgga tggctttcgc
    14641 gtaaaaattc cgcaatgtcg atttcgggat ggtggatttg cagtccggcg agcgttgcgc
    14701 cgtaacggtg ccaatagtct tgacgcaggt cggacgcggc ggattcggag agtttgaggc
    14761 ggcgtgccat atagcgcgtc atagcgcggt tgatgaggtg gaagatgcct gcgtcggcat
    14821 cgtgcagcgt gttgtcgagg tcgaacagcc acacggtcgg gttttcttgc attttgaacc
    14881 gtgaaaattt gttagaatgt tattttacag cgaataaagg aagactcgga atgaaacgga
    14941 aaatttggct gctgccgctg ctggcggttt cggcatacct gcaggcgcag acggaagtca
    15001 ggctggcggt gcataagtcg ttcagcctgc ccaaagggct gattgcgcgc ttcgagcggg
    15061 caaacgatgc gaaagtgtcg attattcagg cgggcggcgc gaacgaaatg ctcaacaaac
    15121 tgattttgag ccgagccaac ccgattgccg atgcggtgta cggtttggac aacgccaata
    15181 tcggcaaggc gcgggaaatg ggcattttgg cggcggcgca acccgaatcc gcccccgtcg
    15241 cggtcgggct gccttcggct ttggcggtcg attacggcta tgtgtccgtc aattacgaca
    15301 aaaaatggtt tgaaggcaaa aagctgcccc tgccgcaaac cctgcaggat ttgacccgcc
    15361 ccgaatataa aaacctattg gtcgtgccgt cccccgccac atcttccccc ggcttgggct
    15421 tcctgatggc gaacatcggc ggtctgggcg aagaaggcgc gtttaaatgg tgggcacaga
    15481 tgcggcagaa cggcgtgaag gtcgccaaag gttggagcga ggcgtattac accgactttt
    15541 cgcacaacgg cggcgcgtat ccgctggtgg tcggttatgc cgccagccct gcggcggaag
    15601 tatatttttc caaaggcaaa tacagcgagc cgccgacggg caacctgttt ttaaaaggcg
    15661 gcgtattccg tcaggtcgaa ggcgcggcgg tcttgaaggg cgcgaaacag ccgaagctgg
    15721 cggcaaaact ggtgcaatgg ctgcaaagtc tggaagtgca gcaggcggtt ccgtctgaaa
    15781 tgtgggttta ccccgccgtc aaaaacacgc gcctgcccga cgtgttccgc ttcgcccaag
    15841 ccccggcgca cactaccgcc cccgcgcagc gcgatattga tgcgaaccag cgcggatggg
    15901 tttcccgttg gatcaggacg gttttgaaat aaaacaaaca taccgccccg cagagcttca
    15961 gacggcattt ttacacctgt gccgattacg ccgcacgggg cggatgttcg atcgagagga
    16021 aaacaatgga cttcaaacaa tttgattttt tacacctgat cagtgtttcc ggttgggggc
    16081 atctggctga aaaggcgtgg gcgttcgggc tgaaccttgc cgccgcgctg cttattttct
    16141 tggtcgggaa atgggcggcg aaacgcattg tcgccgtgat gagggcggcg atgacgcgcg
    16201 cgcaggtcga tgccacgctg attagttttt tgtgtaatgt tgccaatatc ggcttattga
    16261 ttttggtgat tattgccgca ttgggacggt tgggcgtttc cacaacatcc gtaaccgcct
    16321 taatcggcgg cgcgggtttg gcggtggcgt tgtccttaaa agaccagctg tccaattttg
    16381 ccgccggcgc gctgattatc ctgttccgcc cgttcaaagt cggcgacttt atccgtgtcg
    16441 gcggttttga aggatatgtc cgggaaatca aaatggtgca gacttctttg cggacgaccg
    16501 acaacgaaga agtcgtgctg cccaacagcg tggtgatggg caacagcatc gtcaaccgtt
    16561 ccagcctgcc gctttgccgc gcccaagtga tagtcggcgt cgattacaac tgcgatttga
    16621 aagtggcgaa agaggcggtg ttgaaagccg ccgccgaaca ccccttgagc gttcaaaacg
    16681 aagagcggca gcccgccgcc tacatcaccg ccttgggcga caatgccatc gaaatcacat
    16741 tatgggcttg ggcaaacgaa gcagaccgct ggacgctgca atgcgacttg aacgaacaag
    16801 tggtcgaaaa cctccgcaaa gtcaatatca acatcccgtt cccgcaacgc gacatacaca
    16861 tcatcaattc ttaaacgccg tctgaaaggg ggggtgggaa atggacgcgt tgcacaccat
    16921 cgcccgaaac ctgacgaaaa aacgtcaaac cgtaagctgt gccgaatcct gcacgggcgg
    16981 aatgcttgcc gccgcattca caagcgttgc aggcagttcg caatggttcg accagagttt
    17041 tgtaacatac agcaacaaag ccaaagaata ccgcttgggc gtgttgcccg aaaccctgct
    17101 cgaacacggc gcggtcagcc gccaaaccgt ctatgagatg gcgcgcggcg cgaaagccgt
    17161 ggctcaggcg gattacgccg tcggtatttc cggcatcgcc ggcccgggcg gcggcgaaag
    17221 caaacccgtc ggcacggttt ggttcgggtt tgcctttccg ggcggaagtt gcgaagcaat
    17281 gcgccgtttt gacggcaacc gcgaatccgt ccgcgcgcag gcggtcgcct tcgcgttgga
    17341 acggttggcc gggctggttg aaaacggcgg cgatgctgtc tgaacaaatc cccgccggat
    17401 aaaaaatgcc gtctgaaacg ttgcaggttt cagacggcat tttgccgggt taggcggcgg
    17461 tgcggcttat ttcactttgc ccttcaacgc gccgtagcct gccgcgtcca tttgttccag
    17521 cgggatgaat ttcaagctcg cgccgttgat gcagtagcgc agtccgcctt tgtcgcgggg
    17581 gccgtcgggg aagacgtgtc ccaagtgcga atcggcggcg cggcttctga cttcggtgcg
    17641 gcgcatattg aagctgaaat catcgtgttc ggtaacggat tttgcatcaa tcgggcgcgt
    17701 gaagctcggc cagccgcagc cggaatcata tttgtcggcg gagctgaaca ggggttcgcc
    17761 gctgacaacg tccacataaa tgccgggttt gaacaaatgg tcgtattcgt ggctgaaggc
    17821 gtattcggtc gcgctgtttt gggtcacttg gtattgctct tcggtcaggg tgcgtttgag
    17881 ttcggcgtca ctcggttttt tatacgttgc cgcgtcgaag cctttgcctt gcggtgcggc
    17941 tttggttttg cccggcagcg gttcgtcggc tttgcggatg tcgatgtggc agtagccgtt
    18001 ggggttttta atcaggtagt cctgatggta ttcctcggcg tcgtagaagt ttttcagcgg
    18061 ttcgttttca acaacgaggg gcagttggta tttttgctgc tcgcgtttga gggcggcggc
    18121 gatgacggct ttttcggcgg ggtcggtgta gtacacgccg ctgcggtatt gcgtgccggt
    18181 gtcgttaccc tgtttgttga ggctggtcgg atcaacgacg cggaaataat attgcaggat
    18241 gtcgtccagg ctgagtttgt cggcatcgta ggtcactttg acggtctcgg catggcccgt
    18301 atggcggtag gacacgtctt cgtagctcgg gttttccgtg ttgccgttgg cgtagccgga
    18361 taccgcgtca accacgccgt cgatgcgttg gaaataggct tccaagcccc agaagcagcc
    18421 gccggcgagg tagatggtgc gcgtgttcat gattgctgaa tcctttttct gagtgtcggg
    18481 tttgtagaac gaatgtttca aactgcccaa atcggcattc gggttgcgga ttaacgccaa
    18541 tgcctgcgct tcgttgatgc tgcctttgac gatgcgctgc acgtcgccgt ctttaccgat
    18601 taacgcccaa gaaggataaa cgctgatatt caggttttgg gcgatcgtgc cgccgttgtc
    18661 ggtaacgacg ggcagcttgg ggtagttcaa accggcatac catttttgaa actcgccgtc
    18721 ttttttctcg tgcaaaaagc cgggggaggc gacggtaatc aggttggcgg agctgaattt
    18781 tgcatcttgc gcccatttct cggcctgtcc caattcggac aaacataaag gacaccagct
    18841 cgcccaaaat ttaatcagcg tcggtttgtc ttttttcaaa taaacactgg cggggcggtt
    18901 gtccgcggtt tttaacgtgg ataaagtgtg cggcacggtc gcggtcccgg catcgacgat
    18961 tttgggcgaa caagcgccca gcgcaagcag gcagccgaac ttggcgcaaa gggaaaagaa
    19021 agtacggtgt ttcattttga tgtttcctgt gtggacggtt tgcatgatta gacgtttgag
    19081 atgccgaaac cttacggccc ggattttcag acaaccttgc cgcgcaaaat acgctacaat
    19141 acgccctatt tcaagtttct aaaattaaaa ggaaaattca atgttcagct tcttccgtcg
    19201 caagaaaaaa caggaaacgc cggctctcga ggaggcccaa gttcaggaaa ccgcagcaaa
    19261 agtagaatct gaagttgctc aaatagttgg aaatattaaa gaagatgtcg aatctttagc
    19321 agaaagcgtc aaagggcggg ccgaatctgc cgttgaaacc gtcagcggtg cggttgaaca
    19381 ggtaaaggaa accgttgccg agatgccgtc tgaagcaggg gaagcggcgg aacgcgttga
    19441 atccgcaaaa gaagctgttg ccgaaaccgt cggcgaggct gtcgggcaag ttcaagaagc
    19501 cgttgcgaca actgaagaac acaagctcgg ttgggctgcg cgtttgaaac aaggcttggc
    19561 caaatcacgc gacaaaatgg cgaaatcgct ggccggcgtg ttcggcggcg gacaaatcgg
    19621 cgaggatttg tacgaagagc tggaaaccgt gctgattacc ggcgatatgg gcatggaggc
    19681 caccgaatac ctgatgaaag acgtgcgcga ccgcgtcagc ctcaaagggc tgaaagacgg
    19741 caacgaattg cgcggcgcgt tgaaagaagc cttgtacgac ctgattaagc cgttggaaaa
    19801 accgctggtc ttgcccgaaa ctaaagagcc tttcgtgatt atgcttgccg gtatcaacgg
    19861 cgcgggcaaa accacgtcta tcggcaaact cgccaaatat ttccaagcgc agggcaaatc
    19921 cgtattgctg gcggcaggcg atactttccg cgccgccgcg cgcgagcagc ttcaggcttg
    19981 gggcgggcgc aacaatgtaa ccgtcatttc acaaaccacg ggcgattccg ccgccgtgtg
    20041 cttcgatgcc gtccaagccg cgaaagcgcg cgggatcgac atcgtgcttg ccgacaccgc
    20101 cggccgcctg cccacgcagc ttcatttgat ggaagaaatc aaaaaagtga agcgcgtgct
    20161 gcaaaaagcc attcccggcg cgccgcacga aattatcgtc gtactcgatg ccaatatcgg
    20221 gcaaaacgcc gtcaaccaag tcaaagcctt tgacgacgca ttggggctga cggggcttat
    20281 cgttaccaaa ctcgacggca cggcaaaagg cggcatcctc gccgcgcttg cttccgaccg
    20341 ccccgtcccc gtccgctaca tcggcgtggg cgaaggcata gacgacctgc gcccgtttga
    20401 cgcgcgcgcg tttgtggacg cactgctgga ttgagccgaa atgccgtccg aaaacggca
//
LOCUS       DAXFZR010000038        19209 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000038 DAXFZR010000000
VERSION     DAXFZR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19209
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.038"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            60..371
                     /gene="rpsJ"
                     /locus_tag="ACFJAS_001154"
     CDS             60..371
                     /gene="rpsJ"
                     /locus_tag="ACFJAS_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC3982418.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(713..826)
                     /locus_tag="ACFJAS_001155"
                     /pseudo
     CDS             complement(713..826)
                     /locus_tag="ACFJAS_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1157..1801
                     /gene="rplC"
                     /locus_tag="ACFJAS_001156"
     CDS             1157..1801
                     /gene="rplC"
                     /locus_tag="ACFJAS_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC3982419.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1801..2421
                     /gene="rplD"
                     /locus_tag="ACFJAS_001157"
     CDS             1801..2421
                     /gene="rplD"
                     /locus_tag="ACFJAS_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC3982420.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFVIEALTAETPKTKVFAEQVKNLALEQVLFVTKRLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2418..2732
                     /gene="rplW"
                     /locus_tag="ACFJAS_001158"
     CDS             2418..2732
                     /gene="rplW"
                     /locus_tag="ACFJAS_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC3982421.1"
                     /translation="MNQQRLTQVILAPVVSEKSNVLAEKCNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRILGRRSDVKKAYVSLADGQELDLEAAAAAAD
                     KE"
     gene            2738..3571
                     /gene="rplB"
                     /locus_tag="ACFJAS_001159"
     CDS             2738..3571
                     /gene="rplB"
                     /locus_tag="ACFJAS_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC3982422.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGISAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGVVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3577..3855
                     /gene="rpsS"
                     /locus_tag="ACFJAS_001160"
     CDS             3577..3855
                     /gene="rpsS"
                     /locus_tag="ACFJAS_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC3982423.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3864..4193
                     /gene="rplV"
                     /locus_tag="ACFJAS_001161"
     CDS             3864..4193
                     /gene="rplV"
                     /locus_tag="ACFJAS_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC3982424.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4203..4895
                     /gene="rpsC"
                     /locus_tag="ACFJAS_001162"
     CDS             4203..4895
                     /gene="rpsC"
                     /locus_tag="ACFJAS_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC3982425.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4879..5295
                     /gene="rplP"
                     /locus_tag="ACFJAS_001163"
     CDS             4879..5295
                     /gene="rplP"
                     /locus_tag="ACFJAS_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC3982426.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGCIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5295..5486
                     /gene="rpmC"
                     /locus_tag="ACFJAS_001164"
     CDS             5295..5486
                     /gene="rpmC"
                     /locus_tag="ACFJAS_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC3982427.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5486..5749
                     /gene="rpsQ"
                     /locus_tag="ACFJAS_001165"
     CDS             5486..5749
                     /gene="rpsQ"
                     /locus_tag="ACFJAS_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC3982428.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5971..6339
                     /gene="rplN"
                     /locus_tag="ACFJAS_001166"
     CDS             5971..6339
                     /gene="rplN"
                     /locus_tag="ACFJAS_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC3982429.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6351..6674
                     /gene="rplX"
                     /locus_tag="ACFJAS_001167"
     CDS             6351..6674
                     /gene="rplX"
                     /locus_tag="ACFJAS_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC3982430.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6684..7223
                     /gene="rplE"
                     /locus_tag="ACFJAS_001168"
     CDS             6684..7223
                     /gene="rplE"
                     /locus_tag="ACFJAS_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC3982431.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7226..7531
                     /gene="rpsN"
                     /locus_tag="ACFJAS_001169"
     CDS             7226..7531
                     /gene="rpsN"
                     /locus_tag="ACFJAS_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC3982432.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7547..7939
                     /gene="rpsH"
                     /locus_tag="ACFJAS_001170"
     CDS             7547..7939
                     /gene="rpsH"
                     /locus_tag="ACFJAS_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC3982433.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7953..8486
                     /gene="rplF"
                     /locus_tag="ACFJAS_001171"
     CDS             7953..8486
                     /gene="rplF"
                     /locus_tag="ACFJAS_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC3982434.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8500..8853
                     /gene="rplR"
                     /locus_tag="ACFJAS_001172"
     CDS             8500..8853
                     /gene="rplR"
                     /locus_tag="ACFJAS_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC3982435.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8872..9390
                     /gene="rpsE"
                     /locus_tag="ACFJAS_001173"
     CDS             8872..9390
                     /gene="rpsE"
                     /locus_tag="ACFJAS_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC3982436.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9383..9568
                     /gene="rpmD"
                     /locus_tag="ACFJAS_001174"
     CDS             9383..9568
                     /gene="rpmD"
                     /locus_tag="ACFJAS_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC3982437.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9570..10004
                     /gene="rplO"
                     /locus_tag="ACFJAS_001175"
     CDS             9570..10004
                     /gene="rplO"
                     /locus_tag="ACFJAS_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC3982438.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            10016..11326
                     /gene="secY"
                     /locus_tag="ACFJAS_001176"
     CDS             10016..11326
                     /gene="secY"
                     /locus_tag="ACFJAS_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC3982439.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQSTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11331..11549
                     /gene="infA"
                     /locus_tag="ACFJAS_001177"
     CDS             11331..11549
                     /gene="infA"
                     /locus_tag="ACFJAS_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC3982440.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11570..11683
                     /gene="rpmJ"
                     /locus_tag="ACFJAS_001178"
     CDS             11570..11683
                     /gene="rpmJ"
                     /locus_tag="ACFJAS_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC3982441.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11749..12111
                     /gene="rpsM"
                     /locus_tag="ACFJAS_001179"
     CDS             11749..12111
                     /gene="rpsM"
                     /locus_tag="ACFJAS_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC3982442.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12131..12526
                     /gene="rpsK"
                     /locus_tag="ACFJAS_001180"
     CDS             12131..12526
                     /gene="rpsK"
                     /locus_tag="ACFJAS_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC3982443.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12546..13166
                     /gene="rpsD"
                     /locus_tag="ACFJAS_001181"
     CDS             12546..13166
                     /gene="rpsD"
                     /locus_tag="ACFJAS_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC3982444.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13192..14178
                     /gene="rpoA"
                     /locus_tag="ACFJAS_001182"
     CDS             13192..14178
                     /gene="rpoA"
                     /locus_tag="ACFJAS_001182"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC3982445.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14202..14570
                     /gene="rplQ"
                     /locus_tag="ACFJAS_001183"
     CDS             14202..14570
                     /gene="rplQ"
                     /locus_tag="ACFJAS_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC3982446.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14717..15430
                     /gene="minC"
                     /locus_tag="ACFJAS_001184"
     CDS             14717..15430
                     /gene="minC"
                     /locus_tag="ACFJAS_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC3982447.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15459..16274
                     /gene="minD"
                     /locus_tag="ACFJAS_001185"
     CDS             15459..16274
                     /gene="minD"
                     /locus_tag="ACFJAS_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC3982448.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16278..16541
                     /gene="minE"
                     /locus_tag="ACFJAS_001186"
     CDS             16278..16541
                     /gene="minE"
                     /locus_tag="ACFJAS_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC3982449.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16545..17465
                     /gene="oxyR"
                     /locus_tag="ACFJAS_001187"
     CDS             16545..17465
                     /gene="oxyR"
                     /locus_tag="ACFJAS_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC3982450.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
     gene            complement(17987..19033)
                     /gene="porB"
                     /locus_tag="ACFJAS_001188"
     CDS             complement(17987..19033)
                     /gene="porB"
                     /locus_tag="ACFJAS_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB.IB"
                     /protein_id="HFC3982451.1"
                     /translation="MKKSLIALTLAALPVAATADVTLYGAIKAGVQTYRSVEHTEGKV
                     SSVETGSEIADFGSKIGFKGQEDLGNGLKAIWQLEQGASVAGTNSGWGNKQSFVGLKG
                     GFGTIRAGSLNSPLKNTGANVNAWESGKYTGEFLEISKMAQREHRYLSVRYDSPEFAG
                     FSGSVQYAPKDNSGSNGESYHVGLNYRNNGFFAQYAGLFQRYGEGTKKMEGYAYNIPS
                     LFVEKLQVHRLVGGYDNNALYASVAAQQQDAKLYQNQIVRDNSHNSQTEVAATVAYRF
                     GNVTPRVSYAHGFKGTVDSADHDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGADK
                     IVSTASAVVLRHKF"
BASE COUNT         5236 a         3687 c         4781 g         5505 t
ORIGIN      
        1 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aattgaagga tattgataaa
       61 tggcaaacca aaaaatccgt atccgcctga aagcttatga ttacgccctg attgaccgtt
      121 ctgcacaaga aatcgttgaa actgcaaaac gtaccggtgc tgttgtaaaa ggcccgattc
      181 ctttgccgac caaaatcgag cgtttcaaca ttttgcgttc tccgcacatg aacaaaactt
      241 cccgtgaaca attggaaatc cgcacccatt tgcgcctgat ggacatcgtg gattggaccg
      301 ataaaactac cgatgcgctg atgaagctgg atttgccggc cggtgttgat gtagaaatca
      361 aagtccaata attcggactg tcgaaaatcc ccaagcaatc aatgcttggg gattttttat
      421 gttatgccgt ctgaaaatat gcggcggcaa acagatttaa ggcaaaacaa caaaatcaaa
      481 aaacagtgcg gtcggtgaaa tcggcttatt ttgtgcctca agaattttca gacggcattt
      541 tttagtgaac aaaacaggat attttttatc cataactttc ctaaggcttg ccccccgaaa
      601 aaaggtagaa tcgggcttgg tttgggcaga agcatccatc tgattctaag gatggatgat
      661 gttttattct caagtaaata aaggaaagat gatgagccta ctaatgtctt taaagatata
      721 gtggattaaa tttaaaccgg tacggcgttg cctcgccttg ccgtactatt tgtactgtct
      781 gcggcttcgt cgctttgtcc tgatttaaat ttaatccact atattttaaa caacaaagaa
      841 ttgaatttgg aagaacggca tgatttggag cgggcggtta agatcttgca ggacacgcac
      901 gtcatcccgg tttgtcggag aaagatgcat acgccgtgat ggtcggatac cgacacttta
      961 aaagcatccg accccatgcc ttctgaagat tcaaaacgct tcagacggca tattgaagat
     1021 atttctgata tttctgttga tatttctttg acttgtcgga tataatgtca agcttggtac
     1081 atttttgcca agtttaactt tgtctgaaag acaggccaat cgcagcctgt ccctttactt
     1141 taaaaggaaa ataatcatga ctttaggtct ggttggacgc aaagttggta tgacccgcgt
     1201 gttcgacgag cagggtgttt ctgttccggt aaccgttttg gatatgtctg ccaaccgcgt
     1261 tacacaagta aaatccaaag atactgacgg ctataccgcc gttcaagtta cctttggtca
     1321 gaaaaaagcc aatcgtgtca acaaagccga agccggacac tttgcaaaag caggtgttga
     1381 agccggtcgc ggtttgattg agtttgcttt gactgaagaa aaactggctg aattgaaagc
     1441 cggtgacgaa atcaccgttt ctatgtttga agtcggtcaa ctggtcgatg taaccggtac
     1501 ctctaaaggt aaaggtttct ccggcacgat caaacgtcat aacttcggtg cccaacgtac
     1561 ttcccacggt aactcccgtt ctcaccgcgt tccaggctct atcggtatgg cgcaagaccc
     1621 gggtcgcgtg ttccccggta agcgcatggc cggccaatac ggcaacacca aagcaactgt
     1681 tcaaaaattg gaagttgtcc gtgttgatgc agaacgccaa ctgctgttgg ttaagggtgc
     1741 tgttccgggt gcggtcaaca gcgatgttgt agttcgtccc agcgtgaaag taggtgcgta
     1801 atggaattga aagtaattga cgctaaagga caagtttcag gcagcctgtc tgtttctgat
     1861 gctttgttcg cccgcgaata caatgaagcg ttggttcacc agctggtaaa tgcctacttg
     1921 gcaaacgccc gctctggtaa ccgtgctcaa aaaacccgtg ccgaagtaaa acactcaacc
     1981 aaaaaaccat ggcgtcaaaa aggtaccggc cgcgcccgtt ccggtatgac ttcttctccg
     2041 ctgtggcgta aaggcggtcg cgcgttcccg aacaaacccg acgaaaactt cactcaaaaa
     2101 gtaaaccgta aaatgtaccg tgccggtatg gcgactatcc tgtcccaatt ggcgcgtgac
     2161 gagcgtttgt ttgtgattga ggcgttgact gccgaaactc ccaaaaccaa agtttttgcc
     2221 gaacaagtaa aaaatttggc tctggagcaa gtgctgtttg taaccaaacg gctcgacgag
     2281 aatgtttact tggcttcacg caacttgcca aacgtattgg ttttggaagc tcaacaagtt
     2341 gatccttaca gcttgctgcg ttataaaaaa gtaatcatca ctaaagatgc ggttgcacaa
     2401 ttagaggagc aatgggtatg aatcaacaac gtttgactca agtaatcttg gcgcctgtcg
     2461 tttctgaaaa aagcaacgta ttggctgaaa aatgcaacca aatgacgttt aaagtcttgg
     2521 caaatgcaac caaacctgaa atcaaagctg ctgtcgagct gctgttcggt gttcaagttg
     2581 cttctgtaac taccgttacc actaaaggca aaactaagcg ttttggtcgt attttgggtc
     2641 gccgcagcga tgttaaaaaa gcttatgtaa gcttggctga cggtcaagag ttggatttgg
     2701 aagccgctgc tgcagctgca gataaggaat aaacaaaatg gcaatcgtta aaatgaagcc
     2761 gacctctgca ggccgtcgcg gcatggttcg cgtggtaaca gaaggtttgc acaaaggtgc
     2821 accttatgca cccttgcttg aaaagaaaaa ttctactgcc ggccgtaaca ataatggtca
     2881 tatcaccacc cgtcacaaag gcggcggtca taaacaccat taccgtgttg tagactttaa
     2941 acgtaacaaa gacggcattt ctgctaaagt agagcgtatc gaatacgatc ctaaccgtac
     3001 tgccttcatc gcactgttgt gctatgcaga cggcgagcgt cgttacatca tcgctcctcg
     3061 cggtattcaa gccggtgtcg tattggtttc cggtgctgaa gctgccatca aagtaggcaa
     3121 caccctgccg atccgcaaca ttcccgttgg tacgactatc cactgtatcg aaatgaaacc
     3181 cggtaaaggt gctcaaatcg cacgttctgc cggtgcttct gcggtattgt tggctaaaga
     3241 aggtgcatac gctcaagtcc gtctgcgctc tggcgaagtt cgtaaaatca acgtagattg
     3301 ccgtgcgacc atcggtgaag tcggtaacga agagcaaagc ctgaaaaaaa tcggtaaagc
     3361 cggtgctaac cgttggcgcg gtattcgtcc gaccgtacgc ggtgttgtca tgaatcccgt
     3421 cgatcacccg catggtggtg gtgaaggccg taccggcgaa gcccgcgaac cggttagtcc
     3481 atggggtact cctgctaaag gctaccgcac tcgtaataac aaacgcacgg ataatatgat
     3541 tgttcgtcgc cgttactcaa ataaaggtta attagtatgg ctcgttcatt gaaaaaaggc
     3601 ccatatgtag acctgcattt gctgaaaaaa gtagatgctg ttcgcgcaag caacgacaaa
     3661 cgcccgatta aaacctggtc tcgtcgttct accattctgc ctgattttat cggtctgacc
     3721 attgccgtgc acaacggtcg cacccatgtg cctgtgttta tcagcgacaa tatggttggt
     3781 cataaattag gcgaattctc attgacccgt acctttaaag gccacctggc cgataaaaag
     3841 gctaaaaaga aataaggtga atcatgagag taaatgcaca acataaaaat gcccgtatct
     3901 ctgctcaaaa ggctcgtttg gtagctgatt tgattcgtgg taaagacgtt gcccaagctt
     3961 tgaatatttt ggctttcagc cctaaaaaag gtgccgagct gatcaaaaaa gtattggagt
     4021 cagctattgc taatgctgag cataataacg gtgcggacat tgatgaactg aaagtggtaa
     4081 ctatctttgt tgacaaaggt ccaagcttga aacgttttca agctcgcgcc aaaggtcgcg
     4141 gtaaccgcat cgaaaaacaa acttgtcata tcaatgtgac agtgggtaac taaggaaaag
     4201 ctatgggaca aaagattaac cctacaggct ttcgcctggc ggtaactaaa gactgggctt
     4261 caaaatggtt tgctaaaagt accgactttt ctactgtttt gaagcaggat atcgatgttc
     4321 gcaattattt gcgtcaaaaa ttggccaatg cttcggttgg tcgagtaatt attgaacgcc
     4381 ctgcaaaatc tgcacgcatt accattcact ccgctcgtcc gggtgtggtt atcggtaaaa
     4441 aaggtgagga tatcgaagtt ttgaaacgtg acttgcaagt cttgatgggt gtacctattc
     4501 atgtaaatat tgaagagatt cgccgtcctg agttggatgc tcaaattatt gctgacggta
     4561 tcgcccagca gttggaaaaa cgcgttcaat tccgtcgtgc tatgaaacga gcaatgcaaa
     4621 atgcaatgcg ttctggtgct aaaggcatta agattatgac ttcaggccgt ctgaatggtg
     4681 cggatattgc ccgtagcgaa tggtatcgtg aaggtcgcgt gccactgcat actttacgtg
     4741 caaatgtaga ttatgcaacc agcgaagcgc acaccacata tggtgtattg ggtctgaaag
     4801 tttgggttta tacggaaggc aatattaaat cttccaaacc tgaacatgag agtaaacaaa
     4861 gaaaggcagg tagacgtaat gctgcagcca actagactga aataccgtaa gcaacaaaag
     4921 ggtcgcaata ccggcatcgc tactcgcggt aataaggtaa gtttcggtga gttcggcttg
     4981 aaagccgtag gtcgtggtcg tttgactgcc cgtcaaatcg aagctgctcg tcgtgcaatg
     5041 acccgtcaca tcaaacgtgg tggttgtatt tggattcgtg tattccctga caaaccgatt
     5101 actgaaaagc ctattcaagt tcgtatgggt ggcggtaaag gtaacgtgga atattacatt
     5161 gccgaaatta aaccaggtaa agtgttgtat gaaatggatg gcgttccaga ggaactggct
     5221 cgtgaagcat tcgagttggc tgctgccaaa ttgcctattc ctacaacctt tgtagtaaga
     5281 caggtgggtc aataatgaaa gcaaatgaat tgaaagacaa atccgttgag cagttgaatg
     5341 cagatttgtt ggacttgttg aaagctcagt ttggcttacg tatgcaaaac gctaccggtc
     5401 aattaggcaa accaagtgaa ttgaaacgtg tacgtcgcga tattgctcgt attaaaaccg
     5461 ttttaactga aaaaggtgct aagtaatgag cgaaactaaa aatgttcgta ctttgcaagg
     5521 caaagtagta agcgacaaaa tggataaaac cgtaacagta ttggttgagc gtaaagtaaa
     5581 acattcgctg tatggtaaga ttattcgatt atctactaaa atccatgccc atgatgaaaa
     5641 taatcaatat ggaattggtg atgtggttgt tatatcggaa tcccgtccat tgtcaaaaac
     5701 taaatcttgg gttgtcagtg agttggttga gaaagcacgt tctatttaag aattaaagca
     5761 acgtgcttgg aatgggaaac gaagtattgc agcaaattta atttgcgtgt aaacttcgtt
     5821 tcctgtcttc cagtttcttc tggaagtttc ttccctttcg ggatccaaga ctggtttact
     5881 tgaaccgcaa ggtttcattt aataagcagc ggctttgctg taagttatct gaaagtggta
     5941 aattaagttg gttaatttaa aggtaataat atgattcaaa tgcagaccat cttagatgtg
     6001 gctgataact ctggtgcgcg tcgcgtaatg tgtatcaagg tattgggcgg atctaagcgt
     6061 cgctacgctt ctgttggcga tattattaaa gtggcagtta aagatgcggt tccgcgtggc
     6121 cgtgtcaaaa aaggcgatgt atataatgcg gtagttgttc gtactgctaa gggtgtgcgt
     6181 cgtcctgatg gtgcgttaat taaattcgat aacaatgccg ccgtgttgct gaataataaa
     6241 cttgaacctt tgggtactcg tatctttggt ccggtaaccc gtgaattgcg tactgagcga
     6301 tttatgaaaa tcgtttcatt ggcacctgaa gtattataag gaatagcacg atgaataaaa
     6361 tcattaaagg cgatagggtt gtagtaattg ctggtaagga taaaggtaag cagggtcaag
     6421 tagttcgagt gttgggtggt aaagttgttg ttgagggcgt taatgttgta aaacgccatc
     6481 aaaaacctaa tccaatgcgt ggcattaagg gcggtattat tactaaagaa atgcctttgg
     6541 atatttctaa tatcgcaatc ctgaatccgg aaactaataa agcggaccgt gttggtatta
     6601 agctgattga aaatgaaggc aaagttaaac gcgttcgttt cttcaaatca aatggctcta
     6661 tcattggagc ataaggagat aacatggctc ggttgagaga gttttataaa gagacagttg
     6721 ttcctgaatt ggttaaacaa tttggttaca aatcagtaat ggaagtcccg cgtattgaaa
     6781 aaatcacctt gaatatgggt gtgggtgagg ctgttgctga caaaaaagtt atggaacatg
     6841 ctgtttccga tttagagaga attgccggtc aaagaccggt tgttactgtt gcccgtaaat
     6901 ctatcgcagg ttttaaaatc cgtgataact atccggttgg ttgcaaagta acattgcgtc
     6961 gtgatcaaat gtttgaattc ttggatcgtt tgattactat tgcattacct cgcgtacgtg
     7021 acttccgtgg tgtgagcggt aaatcatttg atggccgtgg caattacaat atgggtgttc
     7081 gtgagcaaat tattttcccg gaaattgaat acgataaaat tgatgctttg cgtggtttga
     7141 atattactat tactactaca gcaaaaaccg atgaggaagc gaaagcttta ttgtcattgt
     7201 ttaaatttcc gttcaaagga taatcatggc taagaaagca cttattaatc gtgatctgaa
     7261 acgtcaagct ttggctaaaa aatatgcggc taaacgcgcg gcaattaaag cggtaatcaa
     7321 tgattcgaat gcaactgagg aagagcgttt tgaggctcgt ttgaggtttc aatccattcc
     7381 tcgtaatgcg gcacctgtgc gtcaacgtcg tcgttgtgct ttgacaggtc gccctcgtgg
     7441 tactttccgt aaatttggtt tgggtcgtat taaaatccgt gaaatcgcca tgcgtggcga
     7501 aattccgggt gttgttaaag ccagctggta ataggagtaa ttaagaatga gtatgcatga
     7561 tcctatttcc gatatgttga ctcgtatccg caatgcgcaa cgtgctaata aagcagcagt
     7621 tgcaatgcct tcttcaaaat taaagtgtgc tattgcaaag gtattgaaag aagaaggata
     7681 tattgaggac ttcgcagttt catctgacgt aaagtctata ttggaaattc aattaaaata
     7741 ctatgcaggt cgtcctgtaa ttgaacaaat caagcgtgta tctcgccccg gtttgcgtat
     7801 ttataaagcg tctagtgaga ttccaagtgt tatgaatggc ttgggtattg ctatcgttag
     7861 tacttctaaa ggtgtaatga ctgatcgtaa agcacgttct caaggtgttg gtggtgagtt
     7921 gttatgcatt gtagcctagt ggaggaaaag aaatgtcacg tgtcgcaaaa aacccagtga
     7981 cggttcccgc tggtgtagaa gtaaaatttg gaacagaggc attggttatt aagggtaaga
     8041 acggtgaatt gtcttttcct ttgcattctg atgtagccat tgaatttaat gatggcaaat
     8101 tgacttttgt tgcgaataat agcagtaaac aagcaaatgc aatgtctggt actgctcgcg
     8161 cattagtcag caatatggtt aaaggtgttt cagaaggttt tgagaagaaa ttacaattga
     8221 taggcgtggg ttatcgtgct caagcacaag gtaaaatctt gaatctgtct ttgggttttt
     8281 ctcatccgat cgtatatgaa atgcctgaag gtgtctccgt tcaaactcct agccaaacag
     8341 agattgtttt aactggctcg gataaacaag ttgttggtca agttgcttct gagattcgtg
     8401 cgttccgtgc tcctgagcct tataaaggta aaggtgttcg ctatgtaggc gaagtagtgg
     8461 taatgaaaga agccaagaaa aaataattga ggttcactaa tggataaaca tacaacccga
     8521 ctccgtcgtg cacgcaaaac ccgtgctcgt attgcggact tgaaaatggt aagattatgt
     8581 gtgttccgaa gcaataatca tatttatgct caagtaatta gtgctgaagg tgataaagta
     8641 ttggctcaag cctctacatt ggaagctgag gtgcgcggta gtctgaaatc tggaagcaat
     8701 gttgaagcag ctgcaatagt tggtaaacgt attgctgaga aggctaaagc agcaggtgta
     8761 gaaaaggttg cttttgatcg ttcaggtttc caatatcacg gtcgtgtgaa ggctttggct
     8821 gaagctgctc gtgaaaatgg tttaagcttc taaatatttg gagactttca gatggcaaaa
     8881 catgaaattg aagaacgcgg tgacggtctg attgaaaaga tggtcgcagt taaccgtgta
     8941 accaaagtag ttaaaggtgg tcgcattatg gctttctctg cgctaactgt tgttggtgat
     9001 ggagatggtc gtattggtat gggtaaaggt aagtcaaaag aagtgcctgt tgcagtccaa
     9061 aaggcgatgg atcaagcacg acgctctatg attaaggtac cattaaaaaa tggtacgatc
     9121 catcatgagg ttattggtcg acatggtgct actaaagtat ttatgcagcc tgctaaagag
     9181 ggtagtggcg taaaagccgg tggacctatg cgtttggttt ttgatgctat gggcattcat
     9241 aatatttccg ccaaagtgca cggatctact aacccatata atatcgtacg tgcaacatta
     9301 gatggtttgt ctaagttgca tactcctgct gatatcgcag ccaaacgtgg cttgacagtg
     9361 gaagacattt tgggagttaa ccatggctga acaaaaaaag attagggtta cattggttaa
     9421 aagcctgatt ggtacaattg aatctcatcg tgcatgtgca cgcggtttag gtttgcgtcg
     9481 tcgcgagcat acggtagagg ttttagatac ctctgaaaac cgtggtatga ttaataaaat
     9541 cagctacttg ttgaaagtgg agtcttgata tgtttttgaa tacaattcaa cctgctgttg
     9601 gtgctacgca tgctggtcgt cgtgttggac gcggtattgg tagtggtctt ggcaaaacgg
     9661 gtggtcgtgg tcataaaggt caaaagagcc ggtctggtgg gtttcataag gtgggtttcg
     9721 agggtggtca aatgcccttg caacgacgcc tccctaaaag aggttttaaa tctttaacag
     9781 tatcagctaa tgcacagctt cgtttaagtg aactggaatc aattgctgtt aatgagattg
     9841 atattttagt cttaaagcaa gcaggtctga ttgcatctac agtctctaat gttaaagtta
     9901 ttgcttctgg tgaaatttct aaggcagttg ctttgaaggg tattaaagtt accaaaggtg
     9961 cgagagctgc tatcgaggat gttggtggta agattgaaat gtaaggttta atattgtggc
    10021 taatcaacaa acgtcatcag gttcatccaa gtttggagat cttaagaaac gtcttttgtt
    10081 tctatttgga gcattgattg tttttcgaat tggtgcccat atacccgtac ctggggttga
    10141 tgctgttgct ttagctaaat tatacgaaag cgctggaaac ggcatcctgg gaatattgaa
    10201 tatgttttcc ggtgggtcgt tagagcgctt tagtatattt gcaataggaa ttatgccata
    10261 tatttcagct tctattattg tacagctcgc ttctgaaatt ttgccatcat tgaaggcttt
    10321 aaaaaaagaa ggggaggctg gtagaaaggt aattacgaaa tatactaggt atggtactgt
    10381 tttgttagca attcttcaaa gtctaggtgt tgcatctttc gtatttcagc aaggaattgt
    10441 tgtaacaagt tcatttgagt ttcatgtttc cacggtagtt tctttggtaa cgggaaccat
    10501 gtttcttatg tggcttgggg agcaaattac tgaaaggggt atcgggaacg gtatttcttt
    10561 aatcattacg gcaggtattg cttcaggtat tccttcgggt attgcaaagc tggttacact
    10621 gacgaaccaa ggttctatga gcatgcttac ggcgttgctt attgtatttg gtgccttatt
    10681 attaatttat ttggttgtat actttgaaag tgcacagcgg aagattccta ttcattatgc
    10741 aaaacgccag tttaatggta gggcgggtag tcaaagtacg catatgcctt tcaagttgaa
    10801 tatggctggt gttattcccc caatttttgc ttccagtatt attctatttc catctactct
    10861 tttaggttgg tttggttcgg ctgatacaaa tagtgttttg cacaaaatag ctggattgtt
    10921 acaacacggt caattgctgt atatggcttt atttgcagcg acagttattt tcttttgtta
    10981 tttttatacg gctttggttt ttagtcctaa agaaatggca gagaatttaa aaaagagtgg
    11041 tgcttttgtt cctgggatta gacctggtga gcagacctct aggtatttag aaaaagttgt
    11101 attacgtttg acattgtttg gagctcttta tattacaact atttgtttaa ttccagagtt
    11161 cttaactacg gttttaaatg taccttttta tttgggtggc acgtctttgt tgattctagt
    11221 tgttgtaacg atggatttta gtacacaaat aaattcgtat aggcttactc aacagtatga
    11281 taagttaatg actcgttcag aaatgaaatc attttctcgg aaatagaatt atggcgaaag
    11341 aagatactat ccaaatgcaa ggtgaaattc ttgaaacttt acctaacgca acatttaaag
    11401 taaaacttga gaatgaccat attgtattgg gtcatatttc tgggaagatg cggatgcatt
    11461 acattcgtat ttctccggga gataaggtca cagtagagct gacaccttat gatttaacta
    11521 gggctcgaat cgttttcaga gcaagataaa ccaataaaag gaaaataaaa tgcgtgtaca
    11581 accatctgtt aagaaaattt gccgaaattg caagattatt cgtcgaaatc gtgtagttcg
    11641 tgtgatttgt actgatcccc gtcacaaaca gcgtcaaggt taatggaata tttcttttaa
    11701 tgtgattctg tgatatagtg acaaaatttg ctctaaaaag gaaaaaacat ggctcgtatt
    11761 gcaggggtaa atatccctaa taacgcacac atcgtaattg gtcttcaggc tatttacggt
    11821 attggtgcta ctcgtgctaa attgatttgt gaggctgcaa atattgcgcc tgatactaaa
    11881 gcaaaagatt tggacgagac tcaattagat gctttgcgtg atcaagttgc caagtatgaa
    11941 gtagaaggtg atttgcgtcg tgaagtaact atgagtatca aacgattgat ggacatgggc
    12001 tgctatcgtg gcttccgtca tcgtcgcggc ttaccatgcc gcggtcaacg cactcgtaca
    12061 aatgcgcgta ctcgcaaagg tccgcgtaaa gcgattgctg gtaagaaata aattttaagg
    12121 aattttatta atggctaaag caaacacagc ttcacgtgta cgtaaaaaag tacgtaaaac
    12181 cgtgagtgag ggtattgtgc acgttcatgc atctttcaac aataccatca ttacaatcac
    12241 tgaccgtcaa ggcaatgcgt tgtcttgggc tacctctggc ggcgctggtt ttaaaggttc
    12301 tcgtaaaagt acaccatttg cagcacaagt tgcagcagaa gcagctggta aagttgccca
    12361 agagtatggc gttaaaaatt tagaggttcg tattaaaggt ccaggtccag gtcgcgaatc
    12421 ctctgtacgt gctttgaatg ctcttggttt caagattacc agcattactg acgttacccc
    12481 gttgcctcat aacggttgcc gtccgcctaa aaaacgtcgt atttaatatt ggagtgattt
    12541 gaaacatggc acgttatatt ggccctaaat gtaagttggc acgtcgcgaa ggtacggatt
    12601 tgtttttgaa gagtgcgcgc cgctctttgg attctaaatg taaaattgat tccgctcccg
    12661 gtcagcatgg tgcaaaaaaa ccgcgtttgt cagactatgg tttgcagttg cgtgaaaaac
    12721 aaaaaatccg ccgtatttat ggcgtattag aacgtcagtt ccgtcgttat ttcgcagaag
    12781 ctgatcgccg taaaggttct actggcgagt tgctgttgca gttgctggaa tctcgtttgg
    12841 ataatgtcgt ttatcgtatg ggtttcggtt ctacccgagc tgaagcaaga cagcttgttt
    12901 ctcataaggc tatagttgta aatggacaag ttgtcaatat tccttctttc caagtgaaag
    12961 ctggtgatgt tgtctcagtt cgtgaaaaag ccaaaaaaca ggtacgtatt caagaagcat
    13021 tgggtttggc aactcaaatc ggcttgccgg gttgggtttc tgtagatgca gataaacttg
    13081 agggtgtgtt caaaaacatg ccggatcgct ctgaattgac cggtgatatt aatgaacagc
    13141 tggtggtaga gttctactct aaataatgct agctcagtga gggacagtta aatgcagaat
    13201 agcacaaccg aatttttgaa acctcgtcaa attgatgtaa atactttttc tgcaactcgt
    13261 gcaaaagtat ctatgcagcc atttgaacgt ggtttcggtc ataccttagg taatgctttg
    13321 cgccgtatct tactgtcatc catgaatggt tttgctccta ctgaagtagc tattgccggt
    13381 gtattgcacg aatattctac tgttggtggt gttcaggaag atgttgttga cattttgctg
    13441 aatattaaag gtattgtgtt taaactccat ggtcgtagcc aagttcaact tgtgttgaag
    13501 aaatcaggtt caggtgtcgt atctgccggt gatattgagt tgccgcatga tgtagaaatt
    13561 ctgaatcctg gtcatgtcat ttgtcatttg gctgataacg gtcaaattga aatggaaatt
    13621 aaagtagagc aaggtcgtgg ttatcaatct gtttcaggtc gtcaggtagt tcgtgatgag
    13681 aaccgtcaga ttggtgcaat ccagttggat gcgagctttt cgcccatcag ccgtgttagc
    13741 tttgaggttg aacctgcacg tgtagagcag cggacggatc ttgataaatt ggttttggat
    13801 atcgaaaccg acggttctat tgatcctgag gaagctgtac gcagtgcggc acgtattttg
    13861 attgatcaga tgtctatttt tgctgatttg cagggtactc ctgtggagga ggttgaagaa
    13921 aaatcacctc ctatcgaccc tgttcttttg catccggtgg atgatctgga attgacagta
    13981 cgttcagcta attgtttgaa agctgaggat atttattata ttggcgattt gattcaacgc
    14041 actgaaaccg agcttcttaa aacgccgaat ttgggacgta aatctttgaa tgagattaag
    14101 gaagtattgg catctaaagg tttgacactg ggttctaagt tggaagcatg gccacctgta
    14161 ggcttggaaa agccttaatg aagaattaaa ggataattga tatgcgtcat cgtaatggca
    14221 atcgcaaatt aaaccgtacc agcagtcatc gtgctgcaat gctgcgtaat atggcgaatt
    14281 cattattgac tcacgaaact attgtaacaa ctctgcctaa ggccaaggaa ttgcgccgtg
    14341 tagtagagcc gttgattaca ttgggtaaaa agccgtcatt ggcaagccgc cgtttggcat
    14401 ttgaccgtac tcgcgaccgt gatgttgtag taaaactgtt tggcgatttg ggtcctcgtt
    14461 ttactgctcg taacggtggt tatgttcgag tgttgaaata cggattccgt aaaggtgata
    14521 atgcacctct ggcactggtt gaattggttg acaaaccggc tgctgagtaa ttttagtcat
    14581 ataacgccat ctgccgaaaa gcaggtggcg ttatttttgc aatatctgat agataatatg
    14641 gtattggcta ttatgtttaa aatattaatt gaatagctaa ggtttgcgcg gtaaacttac
    14701 atcattaaaa aattctatga tggtttatat aatgaatgcc ttcgatataa agtcgacaaa
    14761 gatggacgta ttgtctatat ctttgcatac atcagacttg tttgatttgg aagatgtgct
    14821 ggtcaaattg ggcaagaagt ttcaagagtc tggtgttgtt ccatttgttc tggatgttca
    14881 agaatttgat tatcccgagt ctttggatct tgctgcattg gtttcgttgt tttcaaggca
    14941 tggtatgcaa attttgggtc tgaagcattc taatgaacgt tgggctgctg tggctatgaa
    15001 gtatcatttg ctgttttgtc tgtctcattc ggaaaatgtt aaagaactgg gtcaggttga
    15061 ggtgcagaaa acggaggatg gtcagaaagc aaggaaaaca gtattgatta catcccctgt
    15121 ccgtaccggt cagcaggttt atgccgaaga tggcgatttg attgttacgg gggcggtcaa
    15181 ccagggggcg gaattgattg cagatggcaa tatgcatatt tatgcgccga tgagagggcg
    15241 tgctttggcc ggcgccaaag gtgatacttc tgcccgcata tttatccact ccatgcaggc
    15301 ggagttggtt tctgtcgcgg ggatttaccg taattttgaa caggatttgc cggaccatct
    15361 gcacaagcag ccggtacaga tattgttgca ggataaccga ttggttatca gtgcaattgg
    15421 ctcagagtaa ttgtttgata ttttaaaaag gaaatattgt ggcaaaaatt attgtagtaa
    15481 cttcaggcaa gggcggtgtc ggtaaaacga ctaccagtgc aagtattgca acaggtttgg
    15541 cattacgcgg atataaaact gcggtaattg attttgatgt gggtttgcgc aacctcgacc
    15601 tcattatggg ttgcgagcgt cgtgtcgttt atgacctgat caatgtcatt cagggtgagg
    15661 cgacgctcaa ccaggctttg attaaagata aaaattgtga aaacctgttt attttgccgg
    15721 cttcccagac tcgggataaa gacgctttga cacgcgaggg cgtagaaaaa gtgatgcagg
    15781 agctgtccgg caagaaaatg ggctttgagt atattatttg cgactctcct gctggtattg
    15841 agcagggtgc attgatggcg ttgtattttg ctgatgaagc cattgtaacg accaatcctg
    15901 aggtttccag tgtgcgtgac tccgacagga ttttgggaat tttgcaaagc aaatcccgta
    15961 aggcggagca aggcggttcg gttaaagaac atctgttaat tacgcgttat tctcccgaac
    16021 gtgtggcaaa aggcgaaatg ctgtctgtac aggatatttg cgatattctg cgtattcctt
    16081 tgctgggtgt gattcccgaa tcccaaaacg tcttgcaggc atccaattcc ggagaaccgg
    16141 tcatccatca ggacagcgta gcggcttccg aggcatataa ggacgttatt gcccgtcttt
    16201 tgggcgagaa ccgtgaaatg cgtttcttgg aagctgagaa aaagagcttc ttcaaacgtc
    16261 tgttcggagg ataaggtatg tcattgatcg aacttttatt cggtagaaag cagaaaacgg
    16321 caaccgttgc ccgcgaccgc cttcaaatca tcattgccca agagcgcgcc caagaaggtc
    16381 agactccgga ttacctgccg actttacgta aagagttgat ggaagtcctg tccaaatatg
    16441 tgaatgtttc attagacaat atccgtattt cccaagaaaa gcaggatggt atggatgtgc
    16501 ttgagttgaa cattactttg ccggaacaga aaaaggtata ggacatgacc ttaaccgaat
    16561 tgcggtacat cgtcgcagtc gcccaagaac gtcatttcgg ccgggcggcg cggcgttgtt
    16621 ttgtcagcca gcccactttg tctattgcca ttaagaaatt ggaagaagag cttgccgtct
    16681 ctttgtttga ccggagcagc aacgatatta ttacgaccga ggcgggggaa cgtatcgttg
    16741 cacaggcgcg taaggtattg aaagaggcgg agcttatcag gcatttggca aatgaagaac
    16801 aaaacgagct ggagggtgcg ttcaaactcg ggctgatttt tacggttgcg ccatacctgc
    16861 tgccgaaact gattgtctcg ttgcgccgta ctgcaccgaa aatgcctttg atgttggaag
    16921 agaattacac gcatactttg accgagtcgc tcaaacgcgg ggacgttgac gcgattatcg
    16981 ttgccgaacc gtttcaagag ccgggcattg ttaccgaacc cttgtatgac gaaccgtttt
    17041 tcgtgattgt cccgaaaggg cattcatttg aggaactgga tgccgtttcg ccccggatgc
    17101 tgggtgagga gcaggttttg ctgctgacgg aaggcaactg tatgcgggat caggtactct
    17161 caagctgttc cgaattggcg gcgaaacagc gcatacaggg gctgaccaat acattgcagg
    17221 gcagctcgat caatacaatc cgccatatgg tcgccagcgg tttggcaatc agcgtgttgc
    17281 cggcaaccgc gctgaccgag aacgatcata tgctgttcag cattattccg tttgaaggta
    17341 cgccgccaag ccggcgggtc gtattggcgt atcgccgtaa ttttgtccgt ccgaaggcgt
    17401 tgtcggcgat gaaggcggcg attatgcagt cgcagcttca cggggtaagt tttatccacg
    17461 actaggcgca gaaaatgccg tctgaaacgg gtttcagacg gcatttgtta tctttgccgt
    17521 caatcaggca aacctatccg acctacagtt gcttcgaatc gtcattcccg cgcaggcggg
    17581 aatctaggtc tgtcggtgcg gaaacttatc ggataaaacg cttgctccaa tctcgcgttc
    17641 cagattccca ctttcgtggg aatgacggga tcaggtttcc gtatggatag attcgtcatt
    17701 cccgcgcagg cgggaatcta ggtctgtcag tgcggaaact tatcaggtaa aacggtttct
    17761 ccggtcccga gtcctagatt cccactttcg tgggaatgac ggcatatttt gacattgaat
    17821 aaaaaagact aaaaacggaa aacggaaaac ggaaaacgga aaacggaaaa cggaaaacgg
    17881 aaaacggaaa acggaaaacg gaaaacggaa aacagaaaac agaaaacaga aaacagaaaa
    17941 aagcctgtct gacgacaggc tttttgttga taccaatctt tgcagattag aatttgtggc
    18001 gcagaacgac ggcactggca gtcgatacga ttttgtctgc gcctttgcct tcttgcaacc
    18061 agccggcaga aaccaaggca gaagtgcgtt tggagaagtc gtattccgca ccgacaacca
    18121 cttggtcata agtattgtcg tggtctgcac tatcaacagt gcctttgaag ccgtgggcgt
    18181 aagaaacgcg gggcgttacg ttgccgaaac ggtatgccac ggtagcggca acttcggttt
    18241 gagagttgtg cgaattatca cgcactattt gattttgata caatttggca tcttgttgtt
    18301 gtgcggctac ggaggcgtac agggcattat tgtcgtaacc gccgaccaaa cggtgaactt
    18361 gcagtttttc aacaaacaaa ctggggatat tatatgcata tccttccatt tttttagtgc
    18421 cttcgccgta tctttggaac aagccggcgt attgtgcgaa gaagccgttg tttcggtagt
    18481 tcaaaccaac gtggtaagat tcgccgtttg agcctgaatt gtctttaggt gcgtattgta
    18541 cgctgccgct gaagccggca aattcgggag aatcgtagcg tacggacagg tagcggtgtt
    18601 cccgttgggc cattttgctg atttccagaa actcgccggt atatttgccg gattcccaag
    18661 cattgacgtt ggcgccggtg tttttcaggg ggctgttcag gctgccggcg cggatggtgc
    18721 cgaagccgcc cttcaagccg acgaaggatt gtttgttgcc ccagccgctg ttagtgccgg
    18781 cgacggaggc gccttgttcc aactgccaaa tggctttcag gccgttgccg aggtcttctt
    18841 ggcctttaaa gccgattttc gaaccgaagt cggcgatttc gctgccggtt tccacgctac
    18901 ttaccttgcc ttctgtatgt tctacagaac ggtaagtttg tacgccggct ttgatggcgc
    18961 cgtacagggt gacatcggcc gttgctgcaa caggaagggc tgccaaagtc agggcaatca
    19021 gggatttttt cattgctgta ttcctttttt ggttaagaaa tttaagcagg ccgggttttc
    19081 caagccgctt agctttgcat ttaccgccga ggggctcggc ggttacatgt ctgaaactat
    19141 agatgttcct gtctgaaaaa acaaacttct gcctgttttt gtgtggaatc cgatgtgcat
    19201 tttgaaggg
//
LOCUS       DAXFZR010000039        19181 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000039 DAXFZR010000000
VERSION     DAXFZR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19181
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.039"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..365
                     /locus_tag="ACFJAS_001189"
     CDS             <1..365
                     /locus_tag="ACFJAS_001189"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC3982452.1"
                     /translation="ARDIIRFPGFVNNVSEVIKAHHIFVLPSYREGVPRSTQEAMAVG
                     RAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENRAAVRLMGNASYAIAK
                     DKFDAEKVDLKFLDILKA"
     gene            complement(767..1617)
                     /locus_tag="ACFJAS_001190"
                     /pseudo
     CDS             complement(767..1617)
                     /locus_tag="ACFJAS_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            1967..3481
                     /gene="katA"
                     /locus_tag="ACFJAS_001191"
     CDS             1967..3481
                     /gene="katA"
                     /locus_tag="ACFJAS_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC3982453.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            3997..4562
                     /locus_tag="ACFJAS_001192"
                     /pseudo
     CDS             3997..4562
                     /locus_tag="ACFJAS_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            4784..5947
                     /locus_tag="ACFJAS_001193"
     CDS             4784..5947
                     /locus_tag="ACFJAS_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFC3982454.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(6528..8564)
                     /locus_tag="ACFJAS_001194"
     CDS             complement(6528..8564)
                     /locus_tag="ACFJAS_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC3982455.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDGRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMTIYSEDNEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(8652..9524)
                     /locus_tag="ACFJAS_001195"
     CDS             complement(8652..9524)
                     /locus_tag="ACFJAS_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC3982456.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            9790..12180
                     /gene="gyrB"
                     /locus_tag="ACFJAS_001196"
     CDS             9790..12180
                     /gene="gyrB"
                     /locus_tag="ACFJAS_001196"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC3982457.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPTVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            12279..13664
                     /locus_tag="ACFJAS_001197"
     CDS             12279..13664
                     /locus_tag="ACFJAS_001197"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC3982458.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVAEIMEECIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(13735..13833)
                     /locus_tag="ACFJAS_001198"
     CDS             complement(13735..13833)
                     /locus_tag="ACFJAS_001198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC3982459.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(14405..14481)
                     /locus_tag="ACFJAS_001199"
     tRNA            complement(14405..14481)
                     /locus_tag="ACFJAS_001199"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14445..14447),aa:Asp,seq:gtc)
     gene            complement(14514..14589)
                     /locus_tag="ACFJAS_001200"
     tRNA            complement(14514..14589)
                     /locus_tag="ACFJAS_001200"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14554..14556),aa:Val,seq:tac)
     gene            complement(14629..14705)
                     /locus_tag="ACFJAS_001201"
     tRNA            complement(14629..14705)
                     /locus_tag="ACFJAS_001201"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14669..14671),aa:Asp,seq:gtc)
     gene            complement(14738..14813)
                     /locus_tag="ACFJAS_001202"
     tRNA            complement(14738..14813)
                     /locus_tag="ACFJAS_001202"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14778..14780),aa:Val,seq:tac)
     gene            complement(14883..16859)
                     /locus_tag="ACFJAS_001203"
     CDS             complement(14883..16859)
                     /locus_tag="ACFJAS_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC3982460.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            17005..17844
                     /locus_tag="ACFJAS_001204"
     CDS             17005..17844
                     /locus_tag="ACFJAS_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC3982461.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPEKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            18043..19074
                     /gene="gap"
                     /locus_tag="ACFJAS_001205"
     CDS             18043..19074
                     /gene="gap"
                     /locus_tag="ACFJAS_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC3982462.1"
                     /translation="MGIKVAINGYGRIGRQVLRSIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         4594 a         5471 c         5057 g         4059 t
ORIGIN      
        1 tcgcccgcga tattatccgt ttccccggtt ttgtgaacaa tgtttccgaa gtgataaagg
       61 cgcatcatat attcgtattg ccgtcttata gggaaggcgt tccccggagc actcaggagg
      121 caatggccgt cggcagggcg gtgattacga cggatgtccc cggatgcagg gaaacggttg
      181 ccgacaaggt caacggcttc ctgatcgaac cttggaatcc ccgcatcttg gccgaaaaaa
      241 tgatttattt tatcgaaaac agggctgccg tccgcctgat ggggaatgca agttatgcga
      301 ttgccaaaga taaattcgat gccgaaaaag tcgatttgaa atttctcgat attttgaagg
      361 cgtaaacaag gtggtcggct tttgagtttc ggtgatttct gacaaactcc cgccattccc
      421 gcgaaacccc gtcattcccg cgaaagcggg aatccaggtt cgttgagttt cagatgtgta
      481 gggcgggctg tagggtgggc ttcagcccac cgaaaccaat cccgccacca acctcaccgg
      541 ctcaaaccgg cacagcaact tatatcccgt cattctcgcg caggcgggaa tccagccccg
      601 ccggtacaga aacttatcga aaaaaagttt cttcagattt tacgttctag attcccactt
      661 tcgcgggaat gacgggatgc aggtttccgc gcggacggat tcggcattcc cgctttcgca
      721 gaaatgtcgg cttttggttt gttgtcttta taagattatt cggtaattat tctgcggttt
      781 tgcgtttgtg tgctgccgga atctcaaggg attctctgta tttggcaacg gtgcggcggg
      841 aaacctctat accgcggaaa gccagcaggt tggcgagtgc ctcgtcagaa taggacttgg
      901 gcgtggacga ggcaaaaccg gcggcgggaa aggggttgag cgaagcaatg aggccgagtg
      961 cggcttcgag tgtgccgctg tcggtttggg gcaggcgttt tttattcgcg ccggggtttg
     1021 gctgcggttg ccgtcaatgc tgtcgagggc gtttcggacg atatgcaggg cggcgagttt
     1081 ggcagcacat tcgcccgatc tttctatctg caatatcaac gattcggtca catcggcggc
     1141 ggccatgcct gccggatcga attttttcaa tgcggtcagg gcttgtttca gcattgcttc
     1201 atccaacatc cactccaagg gcgtatggtc caggatgtct tcgatgctgt cggtcaggta
     1261 gccctgctcg tcaaggaaat cgataaggat gtggacgcag gcggcttctt ggtcggaaag
     1321 ggggtgttcg cacgcttgcg cgtgcaggta ttgcttgaaa tcttcttcgc cggcgatgtt
     1381 ggacagcata tcttcgcctt cgtctccgcc gatttgacgc gcgggcgcgg tgtaatggct
     1441 gaattcggca tcggaaaatt catccgtttc tttgcgttcg agcagggggt tgtccgacgg
     1501 ccagttttcg acctcgcgtt caagttcgat acccggcatc tgcaatacgc gcaaagattg
     1561 ttgcagccgc tggtcgagct gctgggtctg tttgagcttt attccgatta aggtaatgat
     1621 aatgcggtga aaattgttat tttcagcctg tcggcgcaaa aaaatgccgc aaagcgtcat
     1681 tgcattataa atggttttaa tgagcgggtt cggattccgt ccgataacaa aaaacaaacg
     1741 aaaatggaaa cttgattgct tataacaata ttaaatcaat ttcatagttt taataacgaa
     1801 aatcttggcg tataatcgca tccatagttt ttgcaaaagg aaaataaaat gtcgattcaa
     1861 gaaatttatt acaatcaaga aaccggttac gaatacgctt tccgccaaat cgtactgtaa
     1921 gccgtttgcc ccccatttga ccaacctgag aaaaggaaca agagcgatga ctacctccaa
     1981 atgccccgta acccatctga ccatgaacaa cggcgcgccc gttgccgaca atcaaaacag
     2041 cctgaccgcc ggcccgcgcg gccccctgct gacgcaagat ttgtggctga atgaaaaact
     2101 cgccgacttc gtgcgcgaag tcatccccga acgccgtatg cacgccaaag gttcgggcgc
     2161 gttcggtacg tttaccgtaa cacacgacat caccaaatac acccgcgcca aaattttcag
     2221 cgaagtcggc aaaaaaaccg agatgttcgc ccgtttcacc accgtggcag gcgagcgcgg
     2281 cgcagccgat gcggaacgcg acatccgcgg ttttgcgttg aaattctata ccgaagaagg
     2341 caactgggat gtggtcggca acaacacgcc cgtgttcttc ctgcgcgacc cgcgcaaatt
     2401 ccccgacctg aacaaagccg tcaaacgcga tccgcgcacc aatatgcgtt ccgccacaaa
     2461 caactgggac ttctggacgc tgctgcccga agccctgcac caagttacca tcgtgatgag
     2521 cgaccgcggt attcccgcca gctaccgcca tatgcacggc ttcggttcgc atacctacag
     2581 cttctggaac gaagcgggcg aacgtttttg ggtgaaattc catttccgca gccagcaagg
     2641 gattaaaaac ctgaccaacg aagaagctgc aaaaatcatc gccgacgacc gcgaaagcca
     2701 tcagcgcgac ctgtacgaag ccatcgagcg cggcgagttc ccgaaatgga cgatgtacat
     2761 ccaagtcatg cctgaagccg atgccgaaaa agtgccttat catccgtttg acttgaccaa
     2821 agtttggccg aaaaaagact atccgctgat tgaagtgggc gaattcgagt tgaaccgcaa
     2881 tcccgaaaac ttcttcgccg atgtggaaca atccgccttc gcaccgagca acctcgttcc
     2941 cggtatcggc gcgagcccgg ataaaatgtt gcaagcccgt ttgttcaatt acgccgacgc
     3001 gcaacgttac cgcttgggtg tgaacttccg ccaaatcccc gtcaatcgtc cgcgttgccc
     3061 tgttcacagc aaccagcgcg acggacaagg ccgcgccgac ggcaactacg gcagcctgcc
     3121 gcactacgaa cccaacagct tcggccaatg gcagcaacaa cccgacttcg ccgaaccgcc
     3181 tttgaaaatc aacggcgacg cggcacactg ggactaccgc caagacgatg acgactactt
     3241 cagccaaccg cgcgccttgt tcaacctgat gaacgacgcg cagaaacagg ctttgtttga
     3301 caacaccgcc gcagccatgg gcgatgcgcc cgacttcatc aaataccgcc atatccgcaa
     3361 ctgctaccgt tgcgacccgg catacggtga aggcgtagcc aaagccctcg gactgaccgt
     3421 cgaagacgca caagccgccc gcgcgaccga tcccgcactg ggtcaggccg gtttgctgta
     3481 agggggcatt atgtggatgg aaattgaaga aatcctgtcc cgcgccgtcc gataccggaa
     3541 ataaccgggc ataaaaatgc cgtctgaaac gtcgtttgac cgtttcagac ggcatttcca
     3601 cacctcgccc cgccgtttca gtgggcgttt tttattatag tggattaaat ttaaaccggt
     3661 acggcgttgc ctcgccttgt cctgattttt gttaatccac tataaacgca aaatatcccg
     3721 tcattcccgc accatcccca ccggttcaaa ccggcacgaa aacttttccg cgtcattccc
     3781 gcgaaagtgg gaatccggaa cgtaaaatct aaagaaaccg ttttatccga taagtttccg
     3841 tgccgacggg gctggattcc cactttcgtg ggaatgacgg gatttctgtt tttgattttt
     3901 tgtttttgcg ggaataacgg gatgcgggtt ttcgtgcggc gtttttcgcg ctttgctgtt
     3961 ttgttataat ccgccctttt taaggatggg tgcggtatga gtctttacgc tttgctcttg
     4021 gttgcgctgg gaatgtcgat ggatgcgttt gccgtcgcgc tggcaaaggg tgcggcggtc
     4081 aggatgcccc gcgcaaaatt gcagcaacgg ctttggtgtt cggtacggtt gaagcgttca
     4141 tgccgctggc gggctgggta ggcggttttt atgccaagcc gtttatcagc gaatgagacc
     4201 attgggtggc ttttgtcctg ttgggcggac tgggtctgaa aatgatgcgc gaagggttgt
     4261 ccggcgaggc ggaagatgtg cgcgaaagca aacaggaaag cctatggatg acggttttga
     4321 ctgcttttgg aaccagtatt gattccatga tcgtcggggt gggcttggcg tttatggagg
     4381 taaacatcgc ctttgccgcc gccgtaatcg gtatggcggc tacggtaatg gttaccatcg
     4441 ggctgacggc gggaaaggct tttggcgtat tgttcggcag gcgtgcggaa tttgccggag
     4501 gtttggtgtt gattgccatc ggtacatgga cgctcttatc gcatttgggt ttgattcaat
     4561 gatgtcggaa aataaaatgc cgtctgaagc tttaccggtt tcagacggca ttttgttaaa
     4621 cttgattctc tatcggtatt attgtgtaaa gctattgaaa ataaaatatt gattttccgc
     4681 aaagatggct aaaggtgcga taggtacact gaagaaataa tcggcagaac aggtttgcct
     4741 gccggtttga acaatgattt taacctccca taaggaatac acgatgtctt tcaaactccg
     4801 ttacctcgca tcagtattgg cattgtcttc actgttggcg gcatgcggcg gtcaggaaaa
     4861 gtctgcggca ggcgatgctt ctcctgcttc cgagaccgaa gcggcttccc aagtacaggc
     4921 ctcggaagcc gttccttccg cttcatcggc ctcgcccgaa gaccaagacc ttttgaaacg
     4981 cgcgcaaggt gtattccaac ctttgccgac tgtcgaagaa atgcagaaaa tccgtccgtt
     5041 taccgaagaa caggtcaaac tcggacacca actctggtat gaaccacgcc tttccaaagg
     5101 caataccgta agctgcaact cttgccacaa ccttgcttcc gccggtgtgg acaatatgcc
     5161 gaccagtcag gggcacaaag ggcagttcgg cggacgcaac tcgcctaccg cattgaatgc
     5221 tgcgctgctg ggcagccagt tttgggacgg acgtgccgcc gatgttgaag aacaggctgg
     5281 cgggcctttg gtgaatccgg tggaaatggc gaatgattcg caagaggcgg ctgcagccaa
     5341 aatcgccaaa gttcccgaat atcaagaaat gtttaaaaaa gcttttcctg aagacggcgc
     5401 ggtttcgttt aaaaacatca ctaccgcatt gggtgcgttt gagcgtaccc tgctgacgcc
     5461 gaccaaatgg gacgaatacc tcaaaggcaa cgtcaacgcc ctgagcgaac aggaacgaaa
     5521 aggcgtgcgc gcgttcatgg acaacggctg tattgcctgc cacaacggtg tcaaccttgg
     5581 aggcacgacc ttccagaaat tcggtctggt ccaagggccg tattggaaat tcattgaaga
     5641 tccgaaacgc gacaaaggcc gtgctgacgt aaccaaaaaa accgaagacg aatttttctt
     5701 ccgtgttccg gggttgcgta acgtggctaa aacttatccg tatttccaca acggcagcgt
     5761 gtgggagctg gataaggcag ttaccatcat gggtaaggcg caattgggta aagacattcc
     5821 gaaagaagat gtggataaca tcgtcgtatt cctgaatgca ctttccggca atgtttccga
     5881 atcagcgcgc acgatgccgg aactgcccct gaccgcaccg atggaatcta agccggacaa
     5941 caaataatcc ggccggtacg caaaaatgcc gtctgaaacg ggaatctgtt tcagacggca
     6001 ttttatattt cgtcgcgtca gatacgcagg gtatgccgta gggtgtgctt cagccagccg
     6061 tttccaccaa ttcaaccaac catgtccggg tcttatgcgt ttttcacgaa tgcgcggcgt
     6121 aggcggaggg attggtgggc tgaagcctaa ttcaatggct gcgttgtgta agccatgatg
     6181 cgtttggata attaatcaat attcttgcgc taccattacc atcggctttg ggggtttgta
     6241 cttcggcatt atgataatca atagcctgtt tggattgtat tcccgaatcc gacagtttta
     6301 gattataaaa aggccgtctg aaaaattttc agacggcctt ttatagtaaa tttcaaataa
     6361 aaatatcttt gtcgtcattc ccgcgcaggc gggaatccag acttgtcggc acagaaactt
     6421 atcgggtaaa acggtttctt caattttatg ttccggattc ccgcctgcgc ggggataacg
     6481 attcaggtat ttctacatcg aatccgcttt ttatatcaac ctgactatca agccgcgttg
     6541 tcgaagccgc tgtggcgcag cagtgcgtct atgctcggtt cgcgtccgcg gaaggctttg
     6601 aaggattccg ccgcgctgcg ggagccgccg acggcaagga tttcttgcca gaagcgtttg
     6661 cctgtggcgg cgacgtcgtc gctttcttca aaggcggcgt aggcatcggc gctgaggact
     6721 tcggcccatg cgtagctgta atagcctgcg gaatagccgc cggcgaagat gtggccgaag
     6781 ctgttggcga agcggttgta ttcgggcggt tggatgacgg cgacttcttt gcgcacgctg
     6841 tctaaaacct gctgccagtt tttcagacgg ccttcgttgt cttcgctgta aatggtcatg
     6901 tcaaagaggg cgaattccat ttggcggacg aggaacatgc cgcgttggaa gtttttggcg
     6961 gcgagcattt tgtcgaagag ttctttcggc aggggctcgc cggtttcttc gtgggcggac
     7021 atttgtgcca agacgtcgta ttcccagacg aagttttcca taaactggct gggcagctcg
     7081 actgcgtccc attcgacgcc gttgatgccg gacacgccca gttcgtccac ttgggtaagc
     7141 aggtggtgca gtccgtggcc ggtttcgtgg aagagggtga ggatttcgtc gtggcttaaa
     7201 cgcgcttctt tgccgccgac gggcggggcg aagttgcaga cgaggtaggc ggtgggcagt
     7261 tgcagcgtgc cgtcggcaaa gcggcggcgg cctttgtagt cgttcatcca cgcgccgccg
     7321 cgtttgcctt cgcgtgcgta caaatccata taaacgccgc cgatggtttc gccgttttgt
     7381 tgcaattcaa aatagcgccc gtctttgtgc cagacgggga cggttttttc ggcaaatccg
     7441 atgccgtaga gttttttgat ttgggcgaac agtccgttta atactttgcc gacggggaag
     7501 tattttttga cttcggtttc gctgaatgcg tatttggctt cgcgcagttt ttcgccggcg
     7561 tagctcaagt cccacggctg cgggtcggcg agaccgaggt gttcgcgggc gaaggctttg
     7621 acttcggcga ggtctttttc ggcgtagggt ttggcgcggc gggcgaggtc gtgcaggaag
     7681 tttaaaacct gttcgggcgt gtccgccatt ttggttgcca gcgacaattc ggcgtaattt
     7741 ttaaagccga gcagtttggc ggttttcaat gcgttttcga gcgtgcggtc gatgttggcg
     7801 gtgttgtcga atttgccgtc gtttgaaagt tcgctggcac gggtaacgta ggcgcggtag
     7861 atttgttcgc gcagttcgcg gttgccggcg tattggatga cggcaaggta gtgcggaatc
     7921 tgcaagccga ttttgtaacc tgttttgcct tcgctttgcg cggcggcggc gaacatggcg
     7981 agcgcgtctt cgggaatgcc ggcaagcggt gcggcatcgt caaagtaaat gccgaacgcg
     8041 tcggtcgcgt ctaggacgtt ttgggagaat ttggcggaaa gttgcgcgcc ttcggtttgc
     8101 agttttgcca gttctgcctg ctgttcgggc ggcagttccg cgccgctcaa tacgaaatcg
     8161 cgcaggtcgt gatcgagctt ggttttttgt gcgggggaaa gcgttgcaaa ttcgggggaa
     8221 tttttgatgg ttttgaagcg gttgtacagt tcgatgtctt gtccgatttc ggtgaagaag
     8281 acggtgattt caggcatcag ttcgttatag acggcgcgca gttcgggcgt gtcgacgacg
     8341 gagttgagat gggacacgac gccccaaatc ctgccgacgc gttcggtgat gccggtcaga
     8401 cgctcgacgg tgttcgccca gccggtgtgc gtttgcgctt tgacggcggc gatttgtccg
     8461 cgcgcttcgg cgatggcggt ttggacggcg ggtttgatgt cttcggtttt gatttgatta
     8521 aaacggggtt cttcgcccaa gtggagcagt gcgttgtcaa tcataagatg ggtttccttt
     8581 gcgtgttgtg cttcagacgg catatcggca atatgccgtc tgaagccaga aatggggccg
     8641 gccggggaaa gttaaagtgt ttcggagccg gcgggatgat ttaacaggcg gtattgtacg
     8701 cgcaaaaatt cgtccataac cgcctgttgg atttccagcc gctttgaaac gggggaggcg
     8761 aagcggacga tgatgcggta tgccttgtcg tcgtacggta cgcgggtaac gcgcggcctg
     8821 gcggcgggcg tgataaacag tttttccgcc tgcacgtttt ccaaataccg ctgaatggcg
     8881 gggatgtagg gcgcgcacaa gggctcgagt acggctttca gacggcatac ggcttcatcc
     8941 gaatccaaat ggatgggaac ggggatttcg accgtatgga tgacatagtc gcccaaaata
     9001 ttgtcgcggc gcacggggtg gctcaacaac aggctgttgg ggaaagaaac ggtggttccc
     9061 gcaagctgtc cgaccaaggg gttcggaccg acctgcatca tcagcgtgtt caacagattg
     9121 atgtcgacca cgcgcccgcg caggccgttg atttcgatat agtcgccgac cgagtattgc
     9181 tgggtggcag accttaaaat actgcccgac agacacataa tcagttcttt tgtcgccacg
     9241 acgaccgccg ccgccaccgc aaacatcgac aaagccagcg tttgaatttg cgccgaccag
     9301 ataaatgcca gcgaaaacag caccaaaagc agcgttatat tgcggctggc aaccaaaaac
     9361 cgccgcttgc tttcgatgcc gaaatccgga tgccgtctga agtggatatt caacagaagg
     9421 gcgcgcgcca gcagcaaagc cgcgaccgcc gccacggatt cggccgcctc cgcgcgtatc
     9481 gggacggcat cgggccaagt gttcaacata ttccatattt ccatttccgc gcccctgtcc
     9541 gaaaagtata gggtgtagat tttagtggca aaaaaacgct tttgccactt tgccggcaaa
     9601 ttccgcgccc gtaccggccg caaatgcgcc gcacgccctt tcagacggca tccggatgcg
     9661 gataacgctg aatcaggcgg cgaaatcggc tataatacac aattatatgc gccgtcccgc
     9721 ccgctttttc cacaaggtgc ggactgtttt ggcggcatcg caaaatcttt tcaaaatccg
     9781 gcaaaaaata tgactgaaca aaaacacgaa gaatacggcg ccgacagcat ccaggtgctc
     9841 gaaggcttgg aagcggtacg caaacgcccc ggcatgtaca tcggcgacac gcaggacggc
     9901 agcgggctgc accatatggt gtttgaagta ttggacaacg ccatcgacga agcactcgcc
     9961 ggacattgcg acaaaatcac ggtaacgata cacgccgacc attccgtcag cgtcgccgac
    10021 aacgggcgcg gtatgcccac cggcatccac ccgaaagaag ggcgttccgc cgccgaagtc
    10081 atcatgaccg tcttgcacgc gggcggcaaa ttcgacaaca acagctacaa aatctccggc
    10141 ggcctgcacg gcgtgggcgt atccgtcgtc aacgcgctgt ccgactgggt aacgctgacc
    10201 atctaccgcg acggcaaaga acacttcgtc cgcttcgtac gcggcgaaac cgaagagccg
    10261 ctgaaaattg tcggcgattc cgacaaaaaa ggcacgaccg tgcgcttcct cgccggcacg
    10321 gaaaccttcg gcaatatcga atacagcttc gacatcctcg ccaaacgtat tcgcgaactt
    10381 tcgttcctaa acaacggcgt ggacatcgaa ttgaccgacg agcgcgacgg caagcacgaa
    10441 agcttcgccc tttccggcgg cgtggcgggc ttcgtgcaat acatgaaccg caaaaaaacg
    10501 cccttgcacg aaaaaatctt ctatgcgttc ggcgagaaag acggcatgag cgtcgaatgc
    10561 gcaatgcaat ggaacgacag ctatcaggaa agcgtgcagt gcttcaccaa caacatccct
    10621 cagcgcgacg gcggtacgca cctgaccgcg ctgcgccaag tgatgacgcg caccatcaac
    10681 agctacatcg aagctaacga agtcgccaaa aaagccaaag tggaaaccgc cggcgacgat
    10741 atgcgcgaag gtttgacctg cgtgttgtcc gtcaaactgc ccgaccccaa attctcatcc
    10801 caaaccaaag acaaactggt ttccggcgaa atcggccccg ttgtcaacga agtcatcaac
    10861 caagcactaa ccgacttcct cgaagaaaat ccgaacgaag ccaaaatcat caccggcaaa
    10921 atcgtcgatg ccgcccgcgc acgcgaagcc gcccgcaaag cccgcgaaat cacccgccgc
    10981 aaaggcgtga tggacggctt gggactgccc ggcaaactcg ccgactgcca agaaaaagac
    11041 cctgccctgt ctgaactcta cctcgtcgag ggcgactccg caggcggttc cgccatgcag
    11101 ggccgcgacc gcaaattcca agcgattttg ccgctcaaag gtaaaatttt gaacgtcgaa
    11161 aaagcacgtt ttgaaaaaat gctcgccagc caagaggtcg ccaccctgat taccgcgctg
    11221 ggtgcaggca tcggcaaaga agagttcaac cctgaaaaac tacgctacca ccgcatcatc
    11281 atcatgaccg atgccgacgt ggacggtgcg cacatccgca ccctgctcct gaccttcttc
    11341 taccgccaaa tgcccgaact ggtcgagcgc ggctacattt acatcgccca gccgccgctc
    11401 tacaaagcca aatacggcaa gcaggagcgt tacctcaaag acgaactgga aaaagaccaa
    11461 tggctgctcg gccttgcctt ggaaaaagcc aaaatcgttt cagacggccg caccatcgaa
    11521 ggcgcagaac ttgccgacac cgccaaacaa ttcttgttgg cgaaaaccgt catcgaacag
    11581 gaaagccgct tcgtggacga actcgtcctg cgtgccatgc tgcacgcgtc gcccattgat
    11641 ttgacgtcgt ctgaaaacgc cgataaagcc gttgccgaac tttccggttt gcttgacgaa
    11701 aaagaagccg ccctcgaacg catcgaaggt catgaaggac accggttcat caaaatcacg
    11761 cgcaagctgc acggcaacgt catggtcagc tacatcgaac ccaagttcct caacagcaaa
    11821 gcctaccaaa ccctcaccca aaccgccgcc gcgctcaaag gcttggtcgg cgagggcgcc
    11881 aagctctaca aaggcgagaa cgagtacgac gcggacagct ttgaaaccgc tttggacatc
    11941 ttgatgagcg ttgcccaaaa aggtatgtcc atccaacgat acaaaggttt gggcgagatg
    12001 aaccccgagc agctttggga aaccacgatg gatcccaccg tgcgccgcct gttgaaagtg
    12061 cgcatcgaag atgccattgc cgccgacgaa gtgttcgtta ccctgatggg cgacgaggtc
    12121 gaaccgcgcc gcgcctttat cgaaaacaat gcgctgattg cgcaaaatat cgacgcataa
    12181 gtgccgtttt aaaaaaggag acgggcagct tgccgcgtct ccttttggtt tgtcaaacgg
    12241 aagccatgcc gtctgaaacc gcctttcgga gcaaaaccat gatcagcatt tttgatattt
    12301 tcaaaatcgg tatcggccct tccagttcgc atacggtcgg cccgatgaag gccgccgccg
    12361 cctttgccgc aggcttggac gcgcaaaccg cccgcatcgc catcgacatt tacggctcgc
    12421 ttgccctgac cggatacgga cacggcacat ttgacgcgct gatgctcggt ttggaaggca
    12481 gcctgccgca cgacatcccg cttgccggca ttcccgaacg cctcggacgc atccgcacgc
    12541 agcacatcct ccggctcaac gggcaagaaa tccgcttcat ccccgaccgc gacctgaaca
    12601 tacgcggcaa tcaagtgctg cccaaacacc ccaacggcct gcgatttacc gcctatgctt
    12661 cagacggcac ggtattgaag gaacaggttt attattcggt cggcggcggc tttgtcgtta
    12721 ccgaagaaga cttcgagtgg cagtcggaaa cggaaaaagc cgttccttat ccttacaaca
    12781 gttgtgccgg gctgcttgcc cgatgccgtc tgaaccggct cgacatctcc gaagtcgtgt
    12841 tggcaaacga agccgcgctt gccggatgcg gcgaagccga aatccgccgc cgggttgccg
    12901 gtgttgccga gattatggaa gagtgcatca aacgcggctt gggtgcggac ggcgaactgc
    12961 ccggcggatt gaacgtccgc cgccgcgccc cgcagcttgc cgccaaactc aaagtcctgc
    13021 gcgaaaccga aatcgtcaac acccagctct ggccgatggt gtacgccatg gcggtcaacg
    13081 aagaaaacgc cgccggcgga cgcgtcgtta ccgccccgac caacggcgcg gcaggcatca
    13141 ttcccgccgt attgcattat ttccgcaagt tcaaccccca cgccacacag gaacgtgtcg
    13201 aaaacttcct gctcaccgca ggcgcaatcg gcatcctcta caaaaccaac gcctccattt
    13261 ccggcgcgga tgtcggttgt cagggcgaag tcggcgtagc gtgttcaatg gcggcgggtg
    13321 catacgccga agtcatcggc ggcacgccca aacaagtgga aaacgccgcc gaaatggcaa
    13381 tggaacacca tttgggctta acttgcgacc ccgtcggcgg gttggtgcaa atcccctgca
    13441 tcgaacgcaa cggcatcgcc gccgaaaaag ccctcaaact cggcacgctc gcgctcttgg
    13501 aagacggcac ggacaaaaaa gtctcgctcg acgaagtcat ccgaaccatg ctgcaaaccg
    13561 gccgggacat gaaggcgacc tacaaagaaa cctcgcttgc cggactcgcc gccacgctcc
    13621 ggaaaaaagc cgtccccgta tccgtgcgcg tggtcgagtg ctgaggatgg acagaaacga
    13681 aaatgccgtc tgaaaacagt ttttcagacg gcatttcatt tattttttgc gggtttaccg
    13741 cttcaacgcc aacgtcaaca cgcccgccgt aaccagcccc aagcctatcc attcctgcgt
    13801 gttcgggcgt tcgtccaaga aaaccaccgc catcaggaat gcaaaccggc gttgtttgca
    13861 gcatccggca agaattttta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
    13921 aggcagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta gggaatcgtt
    13981 ctctttgagc taaggcgagg caacgccgta ccggtttttg ttaatccact ataaaaggtc
    14041 ttttctaaaa tatgcagata ttcgaatgtc gaatttgcct tatgattacg gttttctgtc
    14101 ttatccgcct taaaacgccg gtattccgtt tgaaccaaat tatatttgcc gaaacactca
    14161 aaaatctccc ttacctgccc gacagacatc aagacttcgt tgttgtaact gagaaaaata
    14221 tattgaaagt ccgcatccct aatcaaagcc tcaaataccg gaaccacatc cgattttgat
    14281 caagtccgcg ctaaaacaca tcgtacagcg caaaaccgga tgggaatggc aaaatccgga
    14341 aacaaacatt tttcaaagcg tacaaacaaa aaataccaac cggaggttgg tatttcttaa
    14401 cttttggcgc ggcggacggg gctcgaaccc gcgaccaccg gcgtgacagg ccggtactct
    14461 aaccaactga gctaccaccg cgcatccatt gtttgcaaca atgaaaggaa acttggtggg
    14521 tgatgacgga gtcgaaccgc cgacattctg cttgtaaggc agacgctcta ccaactgagc
    14581 taatcacccg tgtataaaaa ataccgacag agtcagtatt ttgaaacttg gcgcggcgga
    14641 cggggctcga acccgcgacc accggcgtga caggccggta ctctaaccaa ctgagctacc
    14701 accgcgcatc cattgtttgc aacaatgaaa ggaaacttgg tgggtgatga cggagtcgaa
    14761 ccgccgacat tctgcttgta aggcagacgc tctaccaact gagctaatca cccttgcgat
    14821 ttgcgaaaac gctattagac caaataaccg ggaatggcgc aagccttatt tcggcatttc
    14881 atttatattc cctgcaagct ccagttttca aaagagataa tttcttcttt tttgcctgcg
    14941 accaccaaca cgtcaccgcc ttccaatgta aacgaggcat ccgggttttc aatccggccg
    15001 gtgcggcggc ggacgaacaa gagtttgatg ccgtaagccg ccatcggaag cgtgccgact
    15061 gttttgccga cggcgtatgc ttctgcagcc aaaggaaagg cgtggcggac ggtttcgcca
    15121 ttttcgccga atcctgcctc atcatcacta ccgacaaaca gtccctccaa cgcggcataa
    15181 cggctgtggc ggatatttgc catcgtctga tagacgtgcc ggtacgacgc gccgttaccc
    15241 aacatcgcat agcctgccag cacgagtccg gtttctttgg tgtccgacac ggcttcttcc
    15301 gcacccatat cggtaaacgt tttcacataa tcgtcattgg tggcgcgcgc atatacgggc
    15361 atattgggat gcatggacag cacattgtct aaaacgtgtt gcgtttcgtg catattgttg
    15421 agcgtaacca ccaccatttt cgcccgtccc agaccggcgg cttccaatac ttccctgcgt
    15481 ttcgcatcgc cgaacgacac cggctcgcct gcacttctgg ctacctgcac gcgtgcgatg
    15541 tccaagtcga gcgcgaaata cggaatatcc tcttgggcga ggacgcgtcc gaccgtctgc
    15601 ccgcccctgc cgaagccgac aatcagcaca tgatcagact tgctcatcgc ttccaccaac
    15661 atgctgtgca gatcgagcga cttcatgtcc cagcttgact tgaccaagcg cccgaccagc
    15721 gcatcgctgc cgcccaagag gaagggcgcg ataatcatcg acagcagaac cgccgccgtc
    15781 gccgcctgtt ccaattctgg cgaaaccata tcaagctgcc cggcaatggc cagcatcacg
    15841 aagccgaact cgccgccttg cgcgagatac aaagccgttt tgaggctgtc gccgaccgaa
    15901 tgcttcattt tgaatgcaat ggcaaacaca accaatgcct tcaacaccag cagcattgcc
    15961 aacagcatca atacctgccg ccagccgccg atcaatgcct gaatgtccag cttcatgccg
    16021 accgtgataa agaagaagcc gagcaaaata tcgcggaacg ggcgaatgtc gtcttcgact
    16081 tggaaacggt attccgtttc cgaaagcaac atgccggcaa cgaatgcgcc caacgccata
    16141 gacaaacctt ccagctcagt cagataagcc acacccaagg ttaccagcag cacattgatc
    16201 ataaagagtt cggacgattt gcgttttgcc accatcctga accatcgcga cataattttg
    16261 ctgccgacga aaaacagcag ccccagcgtc agcagcattt ttgcaaacgc caaacccaag
    16321 gccgcccaaa tatttccgtc ccctccgcca gccagcgcgg gaatcagaat catcagcggc
    16381 acgacggcga tgtcctgcat cagcagcacg cccatcgcca tctgaccgtg cggctgcccc
    16441 aactccgtct tttccgacaa aatccggctc acaatcgccg tggacgacat cgccaacgcg
    16501 cccgatacgg caaacgccca attgaacggc acgcccgtca gcatcagtat gcccattacc
    16561 gacagcatcg taacgccgac ctgcaaaccg cccagaccga acaccagccg cctcatcgcc
    16621 ctcagcttgg gcaatgaaaa ctccaaaccg atgctgaaca tcaggaacac aatcccgatt
    16681 tcgcccaaat aatccgtcgc atggcttttc ggaatcaggc tgagcatacc gggccccgcc
    16741 aaaaagccca ccagcaggta gcccagcatg gagggaatgt tgaacttgcg gcacaggatc
    16801 accgtaatga ccgacaccag cagaacaacc acaatagggg caagcgaaaa ttcgttcatg
    16861 gaccgtccga acaggaaaat acagaaaaat gccgtctgaa acggcatacg ccgccgtatt
    16921 ataacaaaac accgcgcacc atccgaaacg ggcgcggcat acaattctga taaaatacgc
    16981 ccttttgacc cgcaccaccg cacaatgacc gccaccgccg ccgatttaga ccgactcctc
    17041 ccccaaaccc aatgccgcga atgcggctat gaaggctgcc tgccctacgc ccgcgcgatg
    17101 ctgcggggcg aggcgcacaa cctctgcgcc ccgggcggcg caaccgtcgt ccgcgacctt
    17161 gccgccctgc tcggcaaacc ccttgtcgca cctgccaaaa cccaagccaa agcactcgcc
    17221 cggatagacg aaaccgcctg tatcggctgc accgcctgca tccgcgcctg ccctgccgat
    17281 gccattatgg gcgcgggcaa acttatgcac accgtcatca ccgacgaatg caccggctgc
    17341 ggactctgcg tcgccccctg ccccgtggac tgcatccata tgcagcccgt tgccgacacc
    17401 gtcctgccgc gcgcgcgccg tttcagcctg tccgacgaca gccgttttgc cgccgccgaa
    17461 cacgcgcgtg cgcgctacct caaacgcaac gaacgcaaac agcgcgaagc cgacgaacgc
    17521 aaggccatgc ttgccgaacg cgaagccgcc gtccgcaacg cgcgtccgca aacgcccgac
    17581 acaccggaaa aaccggcgtt caaccccgcc gacctcatcg ccaaagccat ggcaaaagcg
    17641 caaacccaac aggatcgcct cgccgccgcc gacaaccgcc aaggctatca ggcaaaacag
    17701 atagccgaag cccgcgaacg cgccgagttg cgccgcgccc aacgcgatat gaaatacggc
    17761 agcgacagcg aaaaagccgc cgccctcgaa tacctcaaac aatacaaagc caaacaggaa
    17821 gccgcacaga ataccgcctc ctgaccctta cccgacatgc cgtctgaagc cgcttcagac
    17881 ggcattttat caagctctcc gtccgccgta cccgtgccgt ccgtatctta ccgcccaccc
    17941 ttccggccgt gccgttttca ataaaatatt aattacacgc cactacaaat ttgctataat
    18001 ccgccccgaa aatctaccca accctcaaca aaggaacaaa ccatgggcat caaagtcgcc
    18061 atcaacggct acggacgcat cggccgccag gttttgcgct ccatctacga ctatcagatt
    18121 caagaccaac tccaaatcgt cgccgtcaac gccagcggca gccttgaaac caacgcccat
    18181 ctgaccaaat tcgacaccgt acacggacgc tttgaagccg acgtatccca cgacggcggc
    18241 aacctcatcg tcaacggcga caaaatcccc ttcttctcga cccgcaaccc tgccgaactg
    18301 ccgtggaaag aactcggtgt cgatttggtc atggaatgca ccggcgcgtt caccagcaaa
    18361 gaaaaagcca aaatccacct cgaaagcggc gcgaaaaaag tcctcatttc cgcaccgggc
    18421 ggcgacgatg tcgatgcaac cgtcgtgtac ggcgtgaacg acagcgtcct gaccgccgac
    18481 atgaccgtcg tttccaacgc ttcctgcacc accaactgcc tctcgccggt tgccaaagtg
    18541 ttgagcgaaa gcgtcggcat cgtcaaaggc gcaatgacca ccatccacgc actgaccaac
    18601 gaccaaaccg ttaccgacgt gcgccacaaa gacctgcgcc gcgcccgcag cggcgtggaa
    18661 aacatgattc cgaccaaaac cggcgcggca aaagccgtcg gtttggtctt gcccgaattg
    18721 aaaggcagac tcgacgggct tgccatccgc gtgccgaccg tcaacgtatc cttggtagat
    18781 ttgagcttcc aagccgcgcg cgacaccaca gtcgaagaaa tcaacgcact gatgaaagcc
    18841 gcctcggaag caggcgcgct caaaggcgtt ttgggctaca acaccctgcc attggtttcc
    18901 atggacttca accacaccac cgaagccagc cacttcgacg caacgctgac caaagtcgtc
    18961 gacggcaaca tggtcaaagt gttcgcgtgg tatgacaacg aatggggctt cagctgccaa
    19021 atgctgaaca ccgcacgccg tatgttcgga cttgaagtgc gcccgctcaa ataagcaaca
    19081 aaccgtcaaa caaaatgccg tctgaaaccc gatgttttca agtttcagac ggcacttttc
    19141 atttttgacc gcgattttac ccggctgtcg tcatttccaa t
//
LOCUS       DAXFZR010000040        19083 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000040 DAXFZR010000000
VERSION     DAXFZR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19083
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.040"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            103..1077
                     /locus_tag="ACFJAS_001206"
     CDS             103..1077
                     /locus_tag="ACFJAS_001206"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC3982463.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(1236..1454)
                     /locus_tag="ACFJAS_001207"
     CDS             complement(1236..1454)
                     /locus_tag="ACFJAS_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC3982464.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            1647..1910
                     /locus_tag="ACFJAS_001208"
     CDS             1647..1910
                     /locus_tag="ACFJAS_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC3982465.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIGRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            2033..3846
                     /gene="ggt"
                     /locus_tag="ACFJAS_001209"
                     /pseudo
     CDS             2033..3846
                     /gene="ggt"
                     /locus_tag="ACFJAS_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574051.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            3910..4074
                     /locus_tag="ACFJAS_001210"
     CDS             3910..4074
                     /locus_tag="ACFJAS_001210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982466.1"
                     /translation="MSNQTKIQAIKQTSEQILAICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            4019..4261
                     /locus_tag="ACFJAS_001211"
     CDS             4019..4261
                     /locus_tag="ACFJAS_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982467.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            4328..5578
                     /gene="glyA"
                     /locus_tag="ACFJAS_001212"
     CDS             4328..5578
                     /gene="glyA"
                     /locus_tag="ACFJAS_001212"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC3982468.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLTNPE
                     DEANLAKVRGQVTALCDKYPVYGN"
     gene            complement(5651..6040)
                     /locus_tag="ACFJAS_001213"
     CDS             complement(5651..6040)
                     /locus_tag="ACFJAS_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3982469.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            6980..7771
                     /locus_tag="ACFJAS_001214"
     CDS             6980..7771
                     /locus_tag="ACFJAS_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFC3982470.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGASADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            7922..8566
                     /locus_tag="ACFJAS_001215"
     CDS             7922..8566
                     /locus_tag="ACFJAS_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC3982471.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            8585..10495
                     /locus_tag="ACFJAS_001216"
     CDS             8585..10495
                     /locus_tag="ACFJAS_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC3982472.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENKKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSRENKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            10517..10883
                     /locus_tag="ACFJAS_001217"
                     /pseudo
     CDS             10517..10883
                     /locus_tag="ACFJAS_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10995..12470
                     /gene="trpE"
                     /locus_tag="ACFJAS_001218"
     CDS             10995..12470
                     /gene="trpE"
                     /locus_tag="ACFJAS_001218"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC3982473.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFDNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            12554..13492
                     /locus_tag="ACFJAS_001219"
     CDS             12554..13492
                     /locus_tag="ACFJAS_001219"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3982474.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDDWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            13493..14353
                     /locus_tag="ACFJAS_001220"
     CDS             13493..14353
                     /locus_tag="ACFJAS_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HFC3982475.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCIYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            14370..15506
                     /locus_tag="ACFJAS_001221"
     CDS             14370..15506
                     /locus_tag="ACFJAS_001221"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC3982476.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKAWIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            15553..16035
                     /locus_tag="ACFJAS_001222"
     CDS             15553..16035
                     /locus_tag="ACFJAS_001222"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3982477.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            16141..17189
                     /locus_tag="ACFJAS_001223"
                     /pseudo
     CDS             16141..17189
                     /locus_tag="ACFJAS_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
     gene            17769..18374
                     /locus_tag="ACFJAS_001224"
     CDS             17769..18374
                     /locus_tag="ACFJAS_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC3982478.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEQDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            18437..18799
                     /gene="ssrA"
                     /locus_tag="ACFJAS_001225"
     tmRNA           18437..18799
                     /gene="ssrA"
                     /locus_tag="ACFJAS_001225"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         5355 a         5084 c         4265 g         4379 t
ORIGIN      
        1 cactacaatc ccacgcgata tccggcggaa cgcaccaaag gaacgccccg cagcaatctg
       61 ccgcctttca gacggcacaa acctttccga aacgagcaaa ccatggacac actgacccga
      121 ttcctccccg aacacctgca acaaaaccaa ctgcccgaag cactcggcgg cgttttgctg
      181 tccgtcgttt ccgcctgtac cgaaatcaac gccaaagtcc gcctcggcgc acttgccggc
      241 gtattgggta tggcgggcac gggcaatatt cagggtgaag accaaaaaaa actggatgtt
      301 atcgccaaca acattatgat tgacacactc aaagccaacc ctgccgttgc cggtttggca
      361 agcgaggaag aagacacttt cgtaagtgcc ggtgaaaacg gacgctatct cgtcctattc
      421 gaccctttag acggatcgtc caatattgat gtcaacattt ccgtcggtac gattttctcc
      481 attctcgcca aacccgaagg cgcattggca accgaatcat tccttcaaac gggcagacag
      541 cagcttgccg caggctatgt cctctacggg cctcaaaccc agctcgtatt cacattcgga
      601 cacggcgtgt acgtatttac actcaatgcc gaaaacgaat ttgtgctgac caaagaaaac
      661 ccgaaagtac ccgaaagtac caaagaattc gccatcaata tgtctaaccg ccgccactgg
      721 ctaccccccg ttcaacaata cgtcgacgag ctcttggcgg gcgaaaccgg tacgcgcggc
      781 aaaaactaca atatgcgctg ggtagccagt atggttgccg aaatccaccg catcctgatg
      841 cgcggcggcg tgttcatgta tctgcaagac aaacgcgacc cgtccaaacc cggcaaactg
      901 cgcctgatgt acgaagccaa ccctatggcc ctgatactcg aacaggcggg cgcatcggca
      961 agcaacgcat atcaagccat gctcgatata cagcccgaaa gcctgcacca acgcgtcgcc
     1021 gtcattatgg gcagcagcga agaagtggat tacctcaacc gtctgcattc aaaataggca
     1081 gagaccgtac tgataaagac cggcggcagc gtgaatccga tactgcgccg atatttcaag
     1141 cataccctcc ggaaaaacag aaaactgcct ttccgaacga taaaaatacc gtctgaaaaa
     1201 ttttcagacg gtattttaaa aattaaagct caaatttatt tgcccgccgc tttttgcagg
     1261 atggcgtaca actcgtcttt cagtttcaat ttggcttttt tcagctcatc gatggtttcc
     1321 gcaccgctgg taaccggatt gttggccaga ccggtaattt tatcgtccag ctcgttatgc
     1381 tcgtcgaaca gacgggcgaa gcgggaattt tcctgtttca atttggaaat caaatcacgg
     1441 tattctggga acatatcgct ttcctctctt ggtttacggt taaaaagtca aattgaatct
     1501 ttacattaat tataacaaag ataatatttt tatccagtgt tttgttatac acggcaacac
     1561 cgtccgcaga aaaccgcccc accgtccgat aaaatcgttg acaatcttcc gctttacgca
     1621 taaattttca gcccttccta ctgcatatga aaggcacgcc ccagtttccc caatgtggat
     1681 tttcagcgcg cgcagtagag gtgccgaccc aaattggcag accctttgct tttgttaata
     1741 tcctagaaaa tccagaaatt cgcagtactc taccactcat cgccaattgg ccaacctttc
     1801 cacggctttg ggttaatggt gagttaatcg gtggtagtga tattatccta caaatgtacc
     1861 aatcaggtga gctaaaacca ctcattgaag agcatagccc aaaggtctga ttacttaata
     1921 tcttaaaata aatatcaaaa aagactgctg atgacattag cggtcttttt ttgatggtca
     1981 agattgatag tttggattga attatttagc catattacat acaataatca gcatgctttg
     2041 tatgaattat caatcaaact caggcgaagg agtgcttgta gctaaaacat atttattgac
     2101 tgcattgata atgtctatgg taatctccgg atgtcaagtc atccatgcca atcaaggtaa
     2161 ggttaatact aattctgctg tcatcgcagg tgcagacgct cacacgcctg aacatgtaac
     2221 gggactgacc gaacaaaagc aggtgattgc aagtgatttt atagtagcgt cagccaatcc
     2281 attagcaaca caagctggct atgatatctt aaagcaaggc ggtagcgctg cagatgcgat
     2341 ggtggcggtg cagacgacac taagcttggt agagccacag tcgtcaggct tgggcggtgg
     2401 tgcatttgtg ttgtattggg acaataccgc caaaacattg accacatttg atgggcgtga
     2461 gacggcaccg atgcgtgcga cgccagaatt atttttggat aaagatggtt aaccattgaa
     2521 atttatggaa gcggtggtcg ctcggtaggt acgcctgcta tccctaaact gacggagaca
     2581 atacatcagc gatacggtgt attgccttgg ggaaaattat ttgatacgcc gatccacttg
     2641 gcaaaacaag gctttgaggt gtcgccaagg cttgccatct cggttgagca aaatcagcag
     2701 catttggcac gctatccaaa aacagccgct tattttttgc cgaatggtgt gccgctacaa
     2761 gcaggcagct tgctgaaaaa tttagaattt gctgacagtg ttcaggcgtt agcagctcaa
     2821 ggtgcaaaag ctctgcatac tggtaaatat gcccaaaata ttgtttcagt tgtccaaaat
     2881 gctaaggata accccggtca attatccttg caaaatttat ctgattatca agtggtggaa
     2941 cgcccgcctg tttgtgtgac ttatcgtatt tatgaagtat gcggtatggg tgcaccaagc
     3001 tcaggtggga ttgctgtggg tcagattttg gggattttaa atgaattttc acccaatcgg
     3061 gtggggtatg atgctgaagg tttgcgtctc ttgggcgatg cttctaggct tgcgtttgcg
     3121 gatcgtgatg tatatttggg cgaccctgat tttgtaccag tacccattcg ccagttgatt
     3181 tctaaagact acctaaaaca tcgcagccag ctgcttgagc agtcggataa ggcattgcct
     3241 agcgtatctg caggcgattt tattcatgag tgggcgtcct cacaagcgat tgagttacct
     3301 tctaccagtc atatatcaat tgtggataag gcgggcaatg tgctatcaat gacgacttcc
     3361 attgaaaatg cgtttggctc aactttgatg gcaaatggct acctgctgaa taatgagctg
     3421 acagacttta gttttgagcc aataaagcag ggcaaacagg tcgccaatcg tgtcgagcct
     3481 ggcaagagac cacgctcctc aatggcacca accatcgtat ttaaagcagg caagccttat
     3541 atggcaatcg gctctccagg tggtagccgt atcattggct atgtcgctaa gacgattgtg
     3601 gcacatagtg actggaatat ggatatccaa gatgccatca gtgcacccaa tttattaaat
     3661 cgctttggta gctatgaact agaaacgggt acaaccgccg tccagtggca acaaacatta
     3721 aatgatttgg gctataagac tgatgtgcgt gagctaaatt caggtgttca agccattatt
     3781 atcgagccaa gcaggctggt tggtggtgtc gatccacgcc gagaaggtcg agtgacgggt
     3841 gattagaggt gaagcttgta ttttgtgaat ttgaccgcat taagttaatt attaaaatag
     3901 ggggaaattg tgagtaatca aacaaaaata caagccatca agcaaacttc tgagcagatc
     3961 ttggcaattt gtgagacgcc aaacacagca ttacaagcca ttcatctgat tttaagacat
     4021 ggcggggcag gaagaattgt cttggcaagt ggtgtatcag cgggttatgg ctgataaaga
     4081 tgtggttggt gctggttatt tgattgattt tgcacaaact gccgaaaatt taccatttga
     4141 tgtattgcct ttgattagcc ttgtgctaaa taaaggtgat gagacgctaa aaaccggcat
     4201 gttaaataaa ttacctgata atgccaaaga aaatttacgc attatgggct atctgcctta
     4261 ggttttgaag tattttttta tacgaaaaac gctcatgata ccctgattac acaaaggagc
     4321 cgagaatatg ttttcaaaaa gcgttaccct cgcacaatat gaccccgatt tggcagcagc
     4381 cattgcccaa gaagaccgac gccagcaaga ccacgtcgag ctgattgcct ctgaaaacta
     4441 cgtcagctgc gccgtgatgg aggcacaagg ttcgcaattg accaacaaat acgccgaagg
     4501 ctatcccgca aaacgctact acggcggctg cgaatacgtc gatattgtcg aacaattggc
     4561 aatcgaccgc gtaaaagaac tgtttggcgc agcctatgcc aacgttcaac cgcactccgg
     4621 ttcccaagcc aaccaagccg tatatgcttc cgttttaaaa ccgggcgaca ccattttggg
     4681 tatgtctctg gcgcacggcg gacacctgac ccacggcgcg agcgttaata tttccggcaa
     4741 actctacaat gccgttactt atggcttgga tgaaaacgaa gtcctcgatt atgccgaagt
     4801 cgaacgcctc gcgctcgaac acaaacccaa aatgattgtg gcgggcgcgt ctgcctacgc
     4861 gttgcaaatc gactgggcaa aattccgcga aatcgccgat aaagtcggcg catacctctt
     4921 tgtcgacatg gcgcactatg ccggactggt tgccggcggc gaatatccta accccgtgcc
     4981 attctgcgac ttcgtaacca ctaccaccca caaaacccta cgcggccctc gcggcggtgt
     5041 gattttgtgc cgtgacaata cccacgaaaa agcgctgaac tcttccatct tcccaagcct
     5101 gcaaggcggt ccgttgatgc acgttatcgc cgccaaagcc gtagcgttta aagaagcgtt
     5161 gcaacccgag ttcaaacaat acgcaaaaca agtgaaaatc aatgctgccg tcatggcgga
     5221 agagttggtt aaacgcggtt tgcgcatcgt ttccggccgc accgaaagcc acgttttcct
     5281 cgtcgacctg caaccgatga aaatcaccgg caaagccgcc gaagccgctt tgggcaaagc
     5341 gcacatcacc gtcaacaaaa acgccatccc gaacgatccg gaaaaaccgt tcgttacctc
     5401 cggcatccgc atcggttccg ccgccatgac cactcgcggc tttaacgaaa ccgacgctcg
     5461 cgtattgtcg aacttggttg ccgatgtatt gactaatccc gaagacgaag ccaacctcgc
     5521 caaagtccgc ggacaagtga ctgccttgtg cgacaaatac cccgtttacg gaaactaaac
     5581 gcccgatgtt tccatacaaa atgccgtctg aacattttcc agacggcatt ttttgttttc
     5641 ggtaacattg ctattttttc tcttccagca aaatgaccaa ctttttcaat actgcgattt
     5701 cactatcttt ctgcctgatc aattcatcct ttgcatccag cagcgacctc aatttttcat
     5761 tttcaaacac cagcatttca tcagagccga taatattatg atggttattg gtattgtcgc
     5821 cgatagagaa gaaaaacgat cggttctgct ccgccagcag ctcaaccaca tcgatattga
     5881 aaatctgggc aatctggcgc agcttgtcga gattgatgcc gcttttcccg cgttctattt
     5941 tcgaataccc gttaaccgac atttccaatt tttccgccat ctcttcctgc gtccactgat
     6001 tgacttccct taacgtccga atcttgtcat gcacttccat cctgccctcc aaggttaatt
     6061 ttcaccactc aaacaaccgt aaccggtatc ttcagttgat ggtcggatat tttaaaaata
     6121 aataatataa cacaatagta ttttgataaa aatttaccta acgaggtaca gacatgtatt
     6181 cagaaaacaa gcctaaacgc accgtaaaaa tgaaaaaata ctggcagcct gcaccctttc
     6241 cctgtccctt tctgcctgta cttcgactca agtaacttct tttgtcgagg gactctccga
     6301 gggactcgta gatgtcagcg aagcctttat cacaacaaag gcagaatcat attctgcccc
     6361 gtctcttcag gataagcaaa ttttcataaa tcgcgtctca gaacgagcac aaaaaaatta
     6421 caaagaaaga aggaaaaaga aagaagcaga cttactaaat tcagcatcgt cattaaatga
     6481 atcaacatca gaagccctat ctgcccttaa aaacgctatc gtaattccta aacgccctaa
     6541 acgctccttg ggcaaaaaat ataaaaacgg ttttcatcct gtaaatacgt gtgcggatgt
     6601 ggaagctcgg tgggcaaacg gagcaatgag agatgttagg gacatagaaa ggctaataag
     6661 ccgaaacgtc cctttacgtc gtatcggaga cgccattcaa aacatgtgca tggaaaccaa
     6721 tgtgtccatt aggatgtatc gccaggagtg ccgttcagaa tcgtcagatt tttatgacga
     6781 aatgcttgag catcacgaga atttcatagg atcctccaaa tcattcgcat catcacttct
     6841 tccccttagc gttacaaact ttacaacaga catggcgtgt actcccgaat tttaatctaa
     6901 tcacactcca caactcccta tcaatataac tgctacttct ctgagtagta tcaaccaacc
     6961 cttaacaaag gactaaaaaa tgaaaaaagc actgcttgca ctgactattg ccgccatctc
     7021 cggtactgct atggcccagt tgcccgactt tctgggtaaa ggcgaatata ccgtccgtac
     7081 agacatctcc aaacaaacgc tgaaaaatgc cgatttgaaa gaaaaacaca aagtacaaaa
     7141 aaacatcggt ttccgtgccg atatgccgtt tgacgatatt caccacggca tgcgtttcga
     7201 agtatcacac agccgagaca aaaaagacat gtacgttgtg accgaaagta ctactaaacc
     7261 attcggcaaa gacgttaaag aaaaacgcac cgacgtgtat gccggttaca cctacactca
     7321 accgctcagt gaagccacca aactgcgtgc cggtttaggt ttgggctatg aaaaatacaa
     7381 agatgcggta gctaatgaga aaggaacagt cagcaccgag cgcgaagcct tctataccaa
     7441 agcgcacgcc gacctgactt ccgatttggg cggcggctgg tatctgaatc cttgggcaga
     7501 ggttaaggtt gacctggacg ccaaattgaa acacaacgca actgtagccg gtgcctcagc
     7561 cgacattaac gcaaaaacac gcggatgggg cgtgggcgta ggtgcgaata tcggcaaaca
     7621 aattaccgat accgtcggca tcgaagccgg cccgttctac aaacaccgtc acttcaaagc
     7681 atccggcagc ttcgttttgg acggcgggaa catccgagtc gatcctacca aaatcaacga
     7741 atacggtgtt cgtgtcggcg tgaaattcta atcgcggcga tatgccccag acacaaaacc
     7801 gcaagccttt tggtttgcgg tttttgatgt tgtagcagac cctaataccg tctgaaactt
     7861 ttcagacggc atttttccaa tcggttaaaa taccgcctcg acattgtgtt ttggaaaacc
     7921 cgtgcttgct tctgtgattg attttatcct gcatatcgac caacacctgc tcagcctgtc
     7981 ggcgcaatac ggtgtgtgga tttatgcgat tctgtttttg attgtttttt gcgaaaccgg
     8041 tctgattgtt acgcccttcc tgcccggcga ttcgctcttg tttgccgccg gcgggattgc
     8101 cgcgctgggc ggtatggata ttcatttgat ggttgcatta ttaagccttg ccgccatact
     8161 cggcgacgcg ctcaatttta ccgtcggcaa atatttcggc ggcaggctgt tcgccaaccc
     8221 cgattctaaa atcttccggt gcgaatacct cggcaaaacc cgccgttttt acgaaaaaca
     8281 cggcggcaaa acgattatca tcgcgcgctt cgtgcccatc gtccgcacct tcgccccctt
     8341 cgtcgccggt atgggaaaaa tgcactatgc caaatttatc cgctacaaca tcatcggcgg
     8401 cttgttgtgg gttatcttgt tttcttacgc gggctatttc tttgccaact tccccgtcgt
     8461 aaaaaacaat ctgggcttgg tgatgggcgg catcattatt gtttccgtcc tgcccggcat
     8521 catcgaaatc gcccgcgcca aacttgcggc aaaatccgaa cgttaattgt tggaaattga
     8581 cattatgaat atattatccg tagaaaacgc ttcctttgcc gtcggacatg tcgccctgct
     8641 cgacaaaacg tccttccaac tcgacagcgg cgaaaaagtc ggcttgatcg gacgcaacgg
     8701 cgcaggcaag tcttcgtttt taaaaatcct caccggcgtg caaaaactcg acgacgggca
     8761 gattattgtt caaaacaatc tcaaaatcgt ttatgtgccg caggagtcct tttttgataa
     8821 ggacgcaacc gtatttgata ctgttgccga aggtttgggc gaaattcgcg atttattgcg
     8881 ccgttatcat catgtcagcc atgagttgga aaatggttcg agtgagctct tattgaaaga
     8941 gcttaacgaa ttgcaacttg aaatcgaagc gaaggacggt tggaaactgg atgcggcagt
     9001 caagcagact ttgggggaac tcggtttgcc ggaaaacaaa aaaatcggca acctttccgg
     9061 cggtcagaaa aaacgcgtcg ccttagcgca ggcttgggta cagaagcccg atgtattgtt
     9121 actggacgaa ccgaccaacc atttggatat cgacgcgatt atttggttgg aaaacctgct
     9181 caaagcgttt gaaggcagct tggtcgtgat tacccacgac cgccgttttt tggacaatat
     9241 cgccacgcgc attgtcgaac tcgatcgcgg tattttgcgc tcctatcccg gctcgttctc
     9301 caaatacagc gagaaaaaag cgcaagagtt ggcggttgaa gccgaacaca accgcctctt
     9361 cgacaaattc cacgcgcagg aagaggcgtg gatacgcaaa ggcatcgaag cgcgccgtac
     9421 ccgcaacgaa ggccgcgtgc gccgtttgga agaactgcgc cgccagcgtg ccgaacgccg
     9481 caatgtacaa ggacaggtca acttcaaact cgacagcggc aagaaaagcg gcaaaatcat
     9541 cgccgaactg gaacacgctt cgtttgccta tgacgacaaa gttatcatgg ataaattttc
     9601 cgccatcttg cagcgcggcg acaaaatcgg cttaatcggc ccaaacggta tcggcaaaac
     9661 caccttcctt aagctgattt tgggcgaatt gcagccgacc tacggcagaa tccgtatcgg
     9721 cagcaagcag gaagtcgcct atttcgacca gttccgcagc gccttgaatg aaaacgatac
     9781 tgtgttttac acgctcggac agggcaacga ttacgtcgaa gtcggcggca agaaaaagca
     9841 tgtgatgagc tatttggaag atttcctgtt cccccctgcc cgcgcgcaaa gtcctgtttc
     9901 ttcactctcc ggcggcgaac gcaaccgcct tttgctggca aaactcttta ctcgtcccgc
     9961 caatattttg gttttggacg aaccgaccaa cgacttggac atcgacaccc aagaattgct
    10021 tgaagatctg ttgcgcgatt accaaggcac ggtattcctc gtctcgcacg accgtatgtt
    10081 cctcgacaat gtaattaccc aaagcattgt tttcgaagga caaggccgtc tgaaagaata
    10141 catcggcggc tatcaggact atatcgacgc aaaatcgcgg gaaaataaaa ttcagacggc
    10201 atccgcaccc aaagcatccg atgtcgaacc cgccaaagaa aaacccaaag ccaaccgtac
    10261 ggtcaaactt tcctacaaag aacagcgcga actcgacgcc ctgcccgacg aaatcgccgc
    10321 cttggaaacc gaacaggcag aaatcaacgc gcagctttcc gatcccggaa ttttcaaaga
    10381 ttacgaaaaa gcaggagcat tgcaaaaccg cgccgaagaa atcgaaatgc tgcttttgga
    10441 aaagctggaa cgctgggagc tgctggaaac caaacagaac ggcaatgccg tctgaaatca
    10501 aacaggaaga aaaaaaatgt tcaaacgacc tgaagaaatc atcgtcctga tacttgccgt
    10561 cctctggatt gccggtacgt attttctcgc cgccctgttt ggagcggatg cttataccgt
    10621 gttaaaaatc actgccctta ccctgctttg gtctgccgca tcattcctcc tttggcaaaa
    10681 aaaacctcag ccggcatatc tggccgctgc tgctcggctg cctgactgcc tgttggtggc
    10741 cgtatctgaa tcaatcggtc gcacccggtt ctttaccttg gcatgcatca tggacgttca
    10801 aaaccatctt agccctgatt cccgcaaccg caggttatct gtttaaatgg aaacaatacc
    10861 ggcagcgcaa aacaaagccg taagcccgtc ttttttcaga cggcatctcc ggtcagactg
    10921 tcaacaattc cgataaaatg ttatactcgc gcccgttgcc gcgccgtccg aaaccgcctt
    10981 ttgggaaagc agaaatgatc agcaaacaag aataccaagc ccaagccgcc caaggctaca
    11041 accgcatccc gctcgtgcaa gaactccttg ccgacttgga tacgccgctt tccctctatc
    11101 tcaaactcgc caaccgcccc tatacctacc tgctcgaatc cgttgtcggc ggcgaacgtt
    11161 tcggccgcta ttcctttatc ggcctgcctt gcagccacta tctcaaagcc ggcggcaaac
    11221 acgtcgatgt ttatcaaaac ggcgaaatcg tcgaacaata cgacggcaat ccgctaccct
    11281 ttatcgaagc tttccacaac cgtttcaaaa cacccgaaat cccaagcctg ccgcgcttta
    11341 ccggcggctt ggtcggctac ttcggttacg aaaccgtcta caatttcgaa cacttcgccc
    11401 accgcctgaa aaacaccgcc aaagccaacc cgctcggcac gcccgatatt ttactgatgc
    11461 tgtcgcaaga gttggcggta atcgacaatt tgagcggcaa aatccacctc atcgtttatg
    11521 ctgatccctc gcagcccgac agctacgaac gcgcccgcga acgcctcgaa gacatccgca
    11581 cccagttgcg ccaaagctgc gccatcccac tttccctcgg cagcaaacaa acccaagcag
    11641 tcagcgagtt cggcgaagaa cctttcaaag cctgcgtcga taaaatcaaa gactacattt
    11701 tcgcaggcga ctgtatgcag gtcgtcccca gccaacgcat gagcatggaa tttaccgaca
    11761 atccgcttgc cctctaccgc gccctgcgca cgctcaaccc ttcgccttac ctcttttact
    11821 acgatttcgg cgatttccac atcgtcggtt cctcgcccga aatcctcgtc cgccgcgaac
    11881 gcgacgacgt catcgtccgc cccatcgccg gcacgcgcct gcgcggcaaa acccccgccg
    11941 aagaccttgc caacgaacaa gatttgttaa gcgacgccaa agaaatcgcc gaacacgtta
    12001 tgcttatcga tttagggcgc aacgatgtcg gacgcatcag caaaacaggc gaagtcaaag
    12061 tcaccgacaa aatggtgatt gaaaaatact cccatgtgat gcacatcgtt tccaacgtcg
    12121 aaggctgtct gaaagagggc gttaccaata tggacatcct cgccgccacc ttccctgccg
    12181 gcacactctc cggcgcgcca aaagtccgcg cgatggaaat catcgaagaa atcgaaccgg
    12241 agaaacgcgg catctacggc ggcgccgtcg gcgtatgggg cttcgacaac gatatggatt
    12301 tggcaatcgc catccgcacc gccgtgatta aaaacaatac cctcttcatc caaagcggcg
    12361 cgggcgtggt tgccgactcc gacccaactt ccgaatggca ggaaactcaa aacaaagccc
    12421 gcgcagtggt tcgggcggcg cagatggtgc aggaagggtt ggataaataa atttcagaca
    12481 acctttgcag atgaaaagaa ggtcgtctga aaatcacaaa acaacatcag agatacttac
    12541 ataaaaaatc acaatgcaat tcacatcatt agaaatttgt gccggagcag gcggacaggc
    12601 tttgggctta gaaagggcag gcttttccca tgttgcgtta atcgaaatcg aaccgtcggc
    12661 ttgtcaaacc ctacgtttaa accgtcctga ttggaacgtt attgaaggag atgtccgctt
    12721 gtttcagggc gaaggatatg acggtattga tttattggcc ggcggcgtgc cttgccctcc
    12781 tttttccaaa gctggaaaac aactgggaaa agatgatgaa cgcgatttat tccctgaggc
    12841 tattcgtcta gctaaggaaa ctgaccctaa agccatcatg ttagaaaatg tgcgggggtt
    12901 acttgaccca aaatttgaaa attatcgcaa ccatattaca gaacaatttg ccaagttggg
    12961 ttaccttgga caatggaaac tactttatgc tgccgattac ggagtatcgc aactcagacc
    13021 cagggtttta tttgttgcgt tgaaaaacga atacaccaat tttttcaaat ggcctgaacc
    13081 aaattcggaa caaccgaaaa ccgttggaga actgcttttt gacttaatgt cagaaaataa
    13141 ctggcagggg gcgcataact ggcgtttgaa ggccgcgcag attgcaccga ccttggtcgg
    13201 cggttcaaaa aaacacggcg gtgctgactt ggggcctaca cgatccaaac gcgcatgggc
    13261 ggagttgggt gtggatggtt caggtttgtg ggatagtgcg ccgcctgaag acttttcggg
    13321 aatgcctaga ttgactgtcc gaatgacggc gcgtatccaa ggatttcctg atgactggca
    13381 attttttggt aaaaaaacgc ctatgtaccg ccaaatcggc aacgcttttc ccccgccggt
    13441 agccgaagcg gtgggcaggc agattatcaa agccttaaaa aaggaaaatt gaatgaatcc
    13501 gctttttacc caagaacgca ggattttcca taagaaatta ttggatggca atattcttgc
    13561 aaccaacaat agaggcgtag tcagcaatgc ggatgggagc aatacccgaa gtttcaatat
    13621 agccaagggg attgccgact tactgcattc ggaaacggtt tcggaaagat tacccggtca
    13681 gacatcggga aatgcttttg aagcaatatg cagtgagttt gtccaatctg cttttgagaa
    13741 attgcagcat atcagacccg gcgactggaa tgttaagcag gtcggttctc gcaaccgtct
    13801 ggaaatcgca cgttatcagc aatacgcaca tctgacagcc ttagccaagg ctgccgaaga
    13861 aaaccccgaa ttggctgccg ccttgggcag tgattacacc attactcccg atattatcgt
    13921 taccagaaat ttgattgcag atgcagaaat caaccgtaat gaatttttag ttgatgaaaa
    13981 tattgccaca tatgccagtt tgcgtgcggg taatggcaat atgccgctgc tccacgccag
    14041 tatctcttgt aaatggacga ttcgcagcga cagggctcaa aatgcccgtt ctgaaggatt
    14101 gaatttggtt agaaacagaa aaggcagact gccgcacatt gttgtcgtaa cggcagaacc
    14161 aacgccaagc cgcatttcat ctattgcctt gggtacaggg gaaatagatt gcatttacca
    14221 ttttgcctta tatgagctgg aacaaatttt acaatcattg aattatgaag acgctttgga
    14281 tttgttttac atcatggtca atggtaaacg attaaaagat atttccgacc ttcccttaga
    14341 tttggcagtt taaacagaat tattccacta tgaacacacc ccccatcctt cctcccgcca
    14401 tgctcggcat cctcggcggc ggacaattag gcagaatgtt tgccgttgcc gctaaaacca
    14461 tgggctacaa agtaaccgtt ctcgatcccg acccgaatgc gccggcggcg gaatttgccg
    14521 accgccattt gtgcgcgccg tttgacgacc gggccgcgtt ggacgaattg gcaaaatgcg
    14581 cggcggttac gaccgaattt gaaaacgtca atgccgacgc gatgcgctct ctggcaaagc
    14641 ataccaacgt ttcccccagc ggcgactgcg tgtccattgc acaaaaccgc attcaggaaa
    14701 aagcgtggat acgcaaagca ggcttgcaaa ccgcgccgta tcaggcggtt tgcaaggccg
    14761 aagacattac tgaagcaagc gcgcaatttt tgcccggcat cctgaaaacg gctacgttgg
    14821 gctacgacgg caaaggtcaa atccgcgtca aaacgttgga cgaactcaaa gccgcgtttg
    14881 ccgaacacgg cggcgtggat tgcgttttgg aaaaaatggt ggacttgcgc ggcgagattt
    14941 ccgtgatcgt atgccgtctg aacgatgaaa acgtgcaaac cttcgacccc gccgaaaaca
    15001 tccacgaaaa cggcatcttg gcttattcca tcgtccccgc gcggctgagt gccgacgtgc
    15061 agcaacaggc gcggcagacg gcgcaacgct tggcggacga attggattat gtcggcgtat
    15121 tggcggtaga aatgtttgtt gtcggcgaca cacatgaatt gctcgtcaat gaaaccgccc
    15181 cgcgcacgca caattccggc caccatacga tagatgcctg cgccgcagac cagttccaac
    15241 agcaggtacg cattatgtgc aacctgccgc ccgccgacac caaattatta tccccttgct
    15301 gtatggcgaa tattttgggc gacgtttggc aggaagatgg cggcgaaccg gattggctgc
    15361 cgttgcaaag ccggccgaat gcacacctgc acctatacgg aaaaaaaacc gcacagaaag
    15421 gtcggaaaat gggacacttt accgttttga ccaccgattc ggacaccgca tttcaagaag
    15481 caaaaaaact gcatcagtcc ctataatccg accaacggca cagatgccgt ctgaagccgc
    15541 cgacataccg ccatgagcct gctgacactg ttacgccccg ccaccgtgca agactgcaaa
    15601 gacatcttca aagtccacct gcactccgtc caatacacct gcatcctcag ctacaacgaa
    15661 cacgccctaa aagtatggga aggcttgtta aacacagaaa gctacctgcc gaccatttcc
    15721 gatcccgaca aagcactttg ggttgccgaa tacaaaggca acatacaggg atttttccaa
    15781 atcgactgcc aagaagcaca actcgatgcg ctttacgtcc accccctgtt ccacaatctc
    15841 ggactgggca ctgccctgct ccgtcaggca gaaaccatcg cccacaaatc cggtttgagc
    15901 tttctgaaac tctacgcatc tctcaactcc gtcccgtttt accttctcaa ccgctacgaa
    15961 tcgctcggtt cggcagtatt gcagcttgac cccagcatca aaatcaaatg cgaactgatg
    16021 cgcaaacacc tctaatcccg ccttataacc aaaactgata atcttcaaat caaacattat
    16081 ttccacaagc gcagacagcc gtataaagtt gccccatcta ccaaccgaca ggacaagcat
    16141 atgaaaacct acgcacaggc actctatacc gcagccctgc ttaccgcctg cagccccgca
    16201 gccgattcaa accatccgtc cggacaaaat gccccggcca ataccgaatc cgacggaaaa
    16261 aacattaccc tgctcaatgc ctcatacgat gtgacacggt atttttacaa agaatacaac
    16321 cacttattta tcaaaacata ccaatccgaa caccccggca catccgtcag catccaacaa
    16381 tcccacggcg gcttcagcaa acaggcatta tccgtagcca acggccttca agccgatgtc
    16441 gtaaccatga accaatcttc cgacatcgac ctgctcgaaa aaaaggactg gtagaaaaag
    16501 gctggcaaca agccctcccc gatcacgccg caccctacac cagcactatg gttttccttg
    16561 tccgaaaaaa caaccccaaa cagatccgcg attggaacga ccttgccaaa gacggcgtta
    16621 acatcgtcat cgccaagacc tcgggcaacg gacgctacgc cttcctcggc gcatacggtt
    16681 acggtctgaa agccaacaac ggcaacgagc aggaagccca aaaactcgtc gcatccatcc
    16741 tcaaaaacac acccgttttt gaaaacggcg gacgcgccgc cgccaccacc ttcacacaac
    16801 gcaacatcgg cgacgtactc atcacttttg aaaacgaagc caactacgtc agcaaaaaac
    16861 tgacccaagg acagtttgaa atcgtctatc cgagctacac catttccgcc gaaagccccg
    16921 ttgccgtcgt caacagcgtc gtcgcgaaaa aaggcacgca aaaaaccgcc cgcgcctatc
    16981 tcgaatacct ttggagcgaa ccggcgcaag aactcgccgc ctcactctac ctgcgtcccc
    17041 gcaaccccga agtattggca cgacacaaag ccgacttccc cgacttagac accttccctc
    17101 ccgaagaaaa attcggcgga tgggacaaca tcatgaaaac ctacttcgcc gacggcggcg
    17161 tattcgaccg cctgaccgca caaaaatgac cattatgccg tctgaaccgc ctttcagacg
    17221 gcatttcaca catcaaaaca gaaaaccgca cccaaaatca tcatcacaag acacaacggc
    17281 aaaccggata caaaacccaa caccgccgcc aaaatgccgt ctgaagccgt cggcgcaacc
    17341 atccttatct ggggcgcgcc tcatcaaagg ggcgcataac tttccttaac catccctttt
    17401 gacagccaaa taaacagtgc ttttgcctgt cagaaacaaa accaatattt tgcggttcgt
    17461 atcggaacat ttataaaaca tcagatttcc gtgtttcgcg tttccggcaa ttcaagatta
    17521 gcaaaaccat taacctgctt cgcaaggcag gtttttaaaa ctttatcagg cgttcgagca
    17581 tatcggttcg accgacccgc atttccggca atcaacactg ccgacggttg ccgcatttct
    17641 ttttgatata caatcacact ttcaaaaaac atacgacggc aacaaaatca tcctcccccc
    17701 gcttggcaaa catccggcaa attaaaaccc cgcccgaaac agccatcgga caaataacag
    17761 gaaaaaatat gaataccgac ttccaaaaga tcctcaaaca actgggcaaa caggccaaaa
    17821 aagaagcggc agaaaaacag gaagccgaaa aaaacaagca aaaacaagaa caggatttgg
    17881 atttttcaca agcagtcgga caagtctccc cattaaaaaa ccggcagcaa tattatgcgc
    17941 agtccgacaa aacccccatc aaagcccgtc ccaaagacaa ccgcaccgac gaagaaaact
    18001 acttctacat aggcagcaca tacaacgacc cacccgccag cttctgcaaa aacggacagg
    18061 gaaaaaacga catccaacgc ctgaaaaacg gatactatcc cgtcgttacc gatgtcgacc
    18121 tgcacggcta cacccaagaa gaagcacaaa aagtcctcaa cgaattcatc gcattcaccc
    18181 aaaaacgcgg cgtatgcggc gaaatcatcc acggcagcgg attaggttcc aaaggctaca
    18241 aacccgttct gaaaaacatg acccgaaact ggctgatgca acaccccgac gtactcgcct
    18301 acgtcgaacc ccgggaaggc aacgacggct gcgtccgcat cctgctcaaa cgcaaattaa
    18361 ggcagcaaga ctgacaaatt tctccgacag acttggcagc accgcacaat atccgtataa
    18421 tttgcaaacc atttctgggg gcgaccttgg tttcgacggg ggttgcgaag cagatgcggg
    18481 cataccgggg tctcagattc ccgtaaaaca ctgaattcaa atagtcgcaa acgacgaaac
    18541 ttacgcttta gccgcttaag gctagccgtt gcagcagtcg gtcaatgggc tgtgtggcga
    18601 aagccaccgc aacgtcatct tacattgact ggtttccagc cgggttactt ggcaggaaat
    18661 aagacttaag gtaactggtt tccaaaaggc ctgttggtcg gcatgatgga aataagattt
    18721 tcaaatagac acaactaagt atgtagaacg ctttgtagag gactttcgga cgggggttcg
    18781 attccccccg cctccaccat gccctatttt cagcagtatc ctgcaacaca aaaaatcctt
    18841 gaacttcaaa agtttcaagg cttttttatt gccttgaata tgatggcgta tgcttgagtt
    18901 ttcgcaattt atggggtaac ttttggggta acttttcagg aatcacccta aaaaatatct
    18961 taaaaataca aaagccatca cgcatttttt aaaaaggtta cccaaaactt aaatgaagcc
    19021 tttatttata aggcttgcag ctttccaaac aagttacccc caaacggata aaaaatgccg
    19081 aag
//
LOCUS       DAXFZR010000041        18994 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000041 DAXFZR010000000
VERSION     DAXFZR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18994
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.041"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            13..831
                     /locus_tag="ACFJAS_001226"
     CDS             13..831
                     /locus_tag="ACFJAS_001226"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC3982479.1"
                     /translation="MPNTKHQIPNTKYIYQDNQGLSEARNTGIKNSNGKYIAFIDSDD
                     FINCQVLLDFLGKDDSDMPDVVFLNAVKYDKGRVSYFGEDYQPEKILNQSKVEVLKGL
                     CRFRKFPGSAWNKIIKRELIIREKLFFEKGIYSEDIEWSMRLFNAATTFSYLDGCYYY
                     YRQGRKDSITGTVSEKGIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTS
                     KNIACDSDIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            872..1402
                     /locus_tag="ACFJAS_001227"
     CDS             872..1402
                     /locus_tag="ACFJAS_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC3982480.1"
                     /translation="MNAIRTFQNRTPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1570..3165)
                     /locus_tag="ACFJAS_001228"
     CDS             complement(1570..3165)
                     /locus_tag="ACFJAS_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC3982481.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3272..3667)
                     /gene="hisI"
                     /locus_tag="ACFJAS_001229"
     CDS             complement(3272..3667)
                     /gene="hisI"
                     /locus_tag="ACFJAS_001229"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC3982482.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3698..4465)
                     /gene="hisF"
                     /locus_tag="ACFJAS_001230"
     CDS             complement(3698..4465)
                     /gene="hisF"
                     /locus_tag="ACFJAS_001230"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC3982483.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIGIREAKRTMREAGIEVRL"
     gene            complement(4478..5215)
                     /gene="hisA"
                     /locus_tag="ACFJAS_001231"
     CDS             complement(4478..5215)
                     /gene="hisA"
                     /locus_tag="ACFJAS_001231"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC3982484.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5248..5886)
                     /gene="hisH"
                     /locus_tag="ACFJAS_001232"
     CDS             complement(5248..5886)
                     /gene="hisH"
                     /locus_tag="ACFJAS_001232"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC3982485.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(6016..7518)
                     /gene="pta"
                     /locus_tag="ACFJAS_001233"
     CDS             complement(6016..7518)
                     /gene="pta"
                     /locus_tag="ACFJAS_001233"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC3982486.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLTQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7703..8761)
                     /gene="fbpC"
                     /locus_tag="ACFJAS_001234"
     CDS             complement(7703..8761)
                     /gene="fbpC"
                     /locus_tag="ACFJAS_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC3982487.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8782..10299)
                     /gene="fbpB"
                     /locus_tag="ACFJAS_001235"
     CDS             complement(8782..10299)
                     /gene="fbpB"
                     /locus_tag="ACFJAS_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC3982488.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10367..11362)
                     /gene="fbpA"
                     /locus_tag="ACFJAS_001236"
     CDS             complement(10367..11362)
                     /gene="fbpA"
                     /locus_tag="ACFJAS_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC3982489.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11614..12094)
                     /locus_tag="ACFJAS_001237"
                     /pseudo
     CDS             complement(11614..12094)
                     /locus_tag="ACFJAS_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(12152..13528)
                     /gene="argH"
                     /locus_tag="ACFJAS_001238"
     CDS             complement(12152..13528)
                     /gene="argH"
                     /locus_tag="ACFJAS_001238"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC3982490.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(13547..14416)
                     /gene="galU"
                     /locus_tag="ACFJAS_001239"
     CDS             complement(13547..14416)
                     /gene="galU"
                     /locus_tag="ACFJAS_001239"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC3982491.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14444..15043)
                     /gene="rdgB"
                     /locus_tag="ACFJAS_001240"
     CDS             complement(14444..15043)
                     /gene="rdgB"
                     /locus_tag="ACFJAS_001240"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC3982492.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSPFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(15036..15269)
                     /locus_tag="ACFJAS_001241"
     CDS             complement(15036..15269)
                     /locus_tag="ACFJAS_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC3982493.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(15377..15910)
                     /locus_tag="ACFJAS_001242"
     CDS             complement(15377..15910)
                     /locus_tag="ACFJAS_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC3982494.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(16012..16470)
                     /gene="nudB"
                     /locus_tag="ACFJAS_001243"
     CDS             complement(16012..16470)
                     /gene="nudB"
                     /locus_tag="ACFJAS_001243"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC3982495.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16681..16757
                     /locus_tag="ACFJAS_001244"
     tRNA            16681..16757
                     /locus_tag="ACFJAS_001244"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16715..16717,aa:Pro,seq:ggg)
     gene            17020..18906
                     /gene="mafB"
                     /locus_tag="ACFJAS_001245"
     CDS             17020..18906
                     /gene="mafB"
                     /locus_tag="ACFJAS_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC3982496.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
BASE COUNT         4100 a         4546 c         5721 g         4627 t
ORIGIN      
        1 accaaacacc aaataccaaa caccaaacac caaataccaa ataccaaata tatatatcag
       61 gataaccaag gattgtcgga ggcgagaaat accggaataa aaaattcaaa tggaaaatat
      121 atagcattta ttgattcgga tgattttatt aattgtcagg ttttgctgga ttttcttggt
      181 aaagatgata gtgatatgcc ggatgtggtt tttttaaatg cagttaaata tgataaggga
      241 agagtttcat attttggcga agattatcag cctgaaaaaa tattaaatca atccaaagtc
      301 gaagttttga aaggattgtg ccgatttaga aaatttccgg gttcggcgtg gaataagatt
      361 ataaaaagag aattgattat tagagaaaaa ctgttttttg aaaagggaat ttattctgaa
      421 gatatcgaat ggtcaatgag gttgtttaat gcggcaacaa ctttttctta tttggacggt
      481 tgttattact attatcggca gggaagaaaa gattctatta cgggaactgt ttcggaaaaa
      541 ggtataaagt cattattata tattttggag aaaaatgcgg aaatggaatt taatagggat
      601 atatcgagtt atctttattc ttttctttcc tacgaatatc tcgttttgct ttttataatg
      661 acgagtaaaa atatagcgtg tgattctgat ataaaaagaa gggcatatca tttaaggttt
      721 atgctgttaa agtccaataa gctgatatat aagctgatat tcccgataat cacattattc
      781 ggggtcgata ttacaggcag gattttaaaa gcaatcaggg ggaatattta ataaatcctt
      841 taacaatata taccttaccg aaggaggaaa aatgaacgca atcagaactt tccaaaaccg
      901 cacgcccgaa atccacgaaa cctgcatgat agacgaggcg tgcgtcgtca tcggcgaagt
      961 ctcgcttgcc gaagatgttt ccgtgtggcc gtgcgccgtg ttgcgcggcg atgtgaacag
     1021 catcaccgtc ggcgcgcgca gcaatataca ggacggcagc gtcctgcacg tttcccacaa
     1081 aaccgccgcc aaacccgaag ggtcgccgct ggttatcggc gaagacgtta ccgtggggca
     1141 caaagtgatg ctgcacggct gccgaatcgg taaccgcgtc ttggtcggta tgggcagcac
     1201 cgttttggac gatgccgtta ttgaagatga cgtgatgatc ggcgcgggca gcctcgttcc
     1261 gccgcgcaaa cgtttggagg gcggctatct ttatatcggt tcgcctgtga ggcaggttcg
     1321 tctgctgacc gatgaggaaa aagccttttt gaaatattcc gccgcacatt atgtgaagct
     1381 gtcgaaacag tacgggatgt gaaatcacat cggcgttctt gcgtcagtcc caaattcacg
     1441 cggttgggat gcccgataac ggtatccgat gcgccttgat tttgaccggc tgcgtttgag
     1501 ttgcaggcaa aaatgccgtc tgaaagcctt tttttcggct tcggacggca ttttattgcc
     1561 gatttctttt tagagtttga ccgaatgttc gcgcgtttcg tggaacacga tgtccggcca
     1621 acgttcttgc gtcaacccca aattcacgcg gttgggggcg aggtaggcga ggttgccgcc
     1681 tgcgtcgatg gcgaggttgc ctgcgttggc tttttcaaat tccgccagtt ttttcttgtc
     1741 gtcgcacgat acccagcgcg ccgaccagat ggatgcgctg tcgaacacgg cttccacgcc
     1801 gtattcgttg gcgaggcgtg aggttacgac ttcaaactgc aacacgccga ccgcacccaa
     1861 aatcaaatcc gcgccgctca tcggtttgaa tacttgaacc gcaccttctt cgccgagttg
     1921 ttgcaaacct ttttgcagtt gtttgatttt cagcgggttt ttgatgcgga cgctgcggaa
     1981 cagttcgggt gcgaagaatg ggatgccggt aaacgccagt tgttcgcctt cggagaagct
     2041 gtcgccgatt tggatgttgc cgtggttcgg gatgccgatg atgtcgccgg cgtaggcttc
     2101 ttccgccagt tcgcggttgt gcgacatgaa ggttactacg ctggaggcgg cgatttcgcg
     2161 gttgatacgc aggtgtttca tcttcatgcc gcgctcgaat ttaccggagc agacgcgcaa
     2221 gaaggcgata cggtcgcggt gtttcgggtc catattggct tggattttaa agataaatcc
     2281 ggaaaatttc ggctcgtccg gcccgaccat gcgcatggtc gcgtcgcgcg gtttcggtgc
     2341 gggtgcccag tcaatcaatg aattgaggat ttcctgaatg ccgaagttgt taatcgcaga
     2401 gccgaagaac actggcgtga gttcgccggc gagaaattcg tcgagattaa attcgttgga
     2461 agccgcctgc accaattcga tttcgtcgcg caactgctgg atttccaacg gaaagcgttg
     2521 ttccaattcg ggattgttta tgcctttgat gatgtcgaac tcgtgcggca ggcgttcgcc
     2581 gcccgcttca aagagataga tttcgtcgtt caggatgtgg tacacgccct tgaagttttt
     2641 gcccataccg atcggccagg taacgggcgc gcagcggatt tgcaggatgt cttccacttc
     2701 gtccaagagt tccaaagaat cgcgcacttc gcggtcgtat ttgttcatga aggtaacaat
     2761 cggcgtatcg cgcaggcggc agacgttcaa gagtttgatg gtttgcgctt ccacgccttt
     2821 tgccgcgtcg atgaccatca aggcactgtc cactgcggtt aaaacgcggt aggtgtcttc
     2881 ggagaagtct tggtgtcccg gcgtgtccaa gaggttgacg gtgtggtctt tgtagtcgaa
     2941 ctgcatcacg cttgatgcca cggaaatgcc gcgctgcttc tcgatgtcca tccagtcgga
     3001 ggtggcgaat ttgccggttt tcttaccttt caccgtgcct gcgctttgaa tcgcgcccga
     3061 aaacagcagc agtttttcgg tcagcgtggt tttacccgca tcggggtggg agatgatggc
     3121 aaacgtgcgg cggcggcgca cttggtcgag gatttcttgg gacatggttt tctttgcaaa
     3181 aaggttcagg ccgcttttca gacggcccgg acagtgtttg agacggcgaa attgtacaaa
     3241 aaaatgcctg ataattcaat gttggaggcg gtcagtgcgt gctgccgtaa atctcttttt
     3301 cgtctttcag gacggcatcg gcggtttccc acgcaccgcc gttccatttt ttgtaaaagc
     3361 agctttcgcg cccggtgtgg caggcgatgc cgccgttttg ggcgatgagc atcacaatgg
     3421 tatcgccgtc gcagtcgagg cgcagttcgc ggactttttg cgtgtgtccc gactcttcgc
     3481 ccttcatcca ttgtttttgg cgcgaacggc tgtaatagtg ggcaaagccg gtttcgacgg
     3541 ttttttgcag ggcttcggcg ttcatccacg ccaccattaa aacacgtttg gtttcggcat
     3601 cttgggcgat ggcgcaaacc aaaccttttt cgtcaaattt gacggcttca agcaggtttt
     3661 tatccatatt tcctttcaga cggcatagtc ggggcggtca gaggcgcact tcgatgccgg
     3721 cttcgcgcat agtgcgtttg gcttcgcgga tgccgatttc cccgaaatgg aaaatgccgg
     3781 cggcaagtac ggcatcggct ttgccttcgg ttatgccttc aatcaggtgc cggacattgc
     3841 cgaccccgcc ggaggcgatg acggggatgt cgacggcttc ggcaacggcg cgggtcagcg
     3901 gcaggttgaa accctgtttc gtcccgtccc tgtccatacc ggtgagcagg atttcgcccg
     3961 cgccgcgttt ttgcatttcg accgcccatt ccaccgcatc caaaccggtc ggattgcgcc
     4021 cgccgtgggt aaagatttcc cagcgtgtgt tttcggggtt gacggctttg gcatcgacgg
     4081 cggcgacgat ggcttgcgaa ccgaaaaatc cggcggcttc gttaattaaa tcgggacggg
     4141 taacggcggc ggtgttgatg ctgactttgt ccgcgcctgc attgagcagg cggcggatgt
     4201 cggcaacggt gcgtacgccg ccgccgacgg tcagcgggat gaagacttgt ccggcaacct
     4261 cttcgatgat gtgcaggatg gtgtcgcggt tgtcggatga ggcggtgatg tcgaggaagg
     4321 tcaattcgtc cgcgccttcg ccgttgtagc gtttggcggc ttcgacgggg tcgcccgcgt
     4381 cgcgcaaacc gatgaagttc acgcctttga cgacgcgccc gtcttttacg tcgagacagg
     4441 ggatgatgcg ttttgccagt gccataatcg gatgccttta gtcgagggaa tctgccagtt
     4501 gctgcgcttg ggcaaaatcg atgctgccct cgtaaatcgc gcggccggta atcgcgcctg
     4561 ctacgccatg tttttcggcg gcacacaggg cgcggatgtc gtccaagccg gtcagtccgc
     4621 cggaggcgat gacgggaatg cggacggatt gggcgagttt gaccgtcgcg tcgatgttca
     4681 cgccgctcat cataccgtcg cgcccgatgt cggtgtagat gatgctgttg acaccgtcgt
     4741 cttcaaagcg ttttgccaaa tcaattacat gatgcccggt tacggttgcc cagccgtcga
     4801 tggcggccat accgtctttg gcatccagtc cgacgataat cctgccgggg aaggctttgc
     4861 acgcttcgcg cacgaagtcg gggtttttga ctgccgccgt gccgataatc acgtcgttca
     4921 gtcccaaatc caaatattgt ccgatggttt tcaaatcgcg catgccgccg ccgagctgca
     4981 cggggatgtc tttggcgacg gcggcgagga tgtctttgat ggcgggcagg ttttgcggaa
     5041 cgccggcaaa tgcgccgttc aaatctacca ggtgcaggcg gcgcgcgcct tgttcgaacc
     5101 agtgcagcgc ggtgtcggcg ggcgaatcgg aaaagacggt cgcctcttcc atcagccctt
     5161 gtttcaagcg gacgcaacgt ccttctttca aatcgatggc gggtatcagc agcatagttt
     5221 ttctccttgt gcggggccgt gtccggctta ccagtttaaa aagtttttca acatcgtcag
     5281 cccggcatcg tggctttttt cggtgtgaaa ttgcgtggcg aatacgttgt ctttgccgac
     5341 gatgcaggca aacggggacg ggtagtcgct ttcgcccaat atggtttcgg gattttcggg
     5401 ggcgaaatag tagctgtgga cgaaataaaa acgcgtgttt tgggggatgc cttgaaaaag
     5461 cgggtggttt tgggtttggc gcacggtgtt ccagcccata tgcgggactt tcagacggca
     5521 tccttgcggg tcgcggaggt cgcgggcaaa gcgtctgact ttgccgccga accagcccaa
     5581 gccgtcggtg tttccttctt cgctgtggtc gaataaaagt tgcgcaccga cgcagattcc
     5641 gaaaaacggt ttgtttttta aggcgtcttt gactgcctcg tccaagccgc cgcgcgtcag
     5701 tgccgccata cagtcgggca tcgcgccctg cccgggaaaa atgactttgt cggcgcggga
     5761 cacgcggtcg gggtcgccgc ttaaaaagat tttggtattt tttccggcaa gctgccccgc
     5821 cgtccggacg gatttcaata cggaatgcag gttgcccata ccgtaatcga taatggcggt
     5881 ttgcatggct tcctcctctt ttttgcaata tggctgcgat tttaacaaac aaatgtgccg
     5941 ggctgataaa aatgccgtct gaaaacggga atctgtcttc agacggcata gggttcaaac
     6001 ccggaaagcc gtttgtcagc cttccatttg ttttgcctga acggcagtca gggcgatggt
     6061 gaacacgatg tcttccacca gcgcgccgcg agacaggtcg ttgaccggtt tgcgcaggcc
     6121 ttgcagcagc gggccgacgc tcaagacgtt ggcactgcgt tggacggctt tataggtgca
     6181 gttgccggtg ttcaggtcgg ggaagaccaa aacggttgcc tgtcctgcca ccgggctgcc
     6241 cggggctttg gatttgccta cgcccggcac ggttgccgcg tcatattgca gcgggccgtc
     6301 gatggcgagg tcggggcgtt tttcccgggc aagtttggtg gcttcgatga cggtatcgac
     6361 atcggggccg ctgccggagt tgacggtgga gtaggaaatc atcgccactt tcgggtcgat
     6421 gccgaaggct tttgcggaat cggcagactg gatggcgatg tcggcaagct gttgcgcggt
     6481 cgggttcgga ttaaccgcgc agtcgccgaa caccagaacc tgatttggta gcagcataaa
     6541 gaatacgctg gatacgaggc ttgcgcccgg tgcggtttta atcagttgca aagcggggcg
     6601 gatggtgttg gcggtggtgt gaaccgcacc ggataccaaa ccgtccacat cattttgcgc
     6661 catcatcatc gtaccgagta ccacggtgtc ttgcagttgc ttgcgcgcgt cttcgggtgt
     6721 caggcctttg gatttgcgca gttcgcacat cggctcgacg tattgttcga ccaatgaggc
     6781 gggatcgatg atttccaaag agtcgggcag gctgatgccg cgttctttgg caacggcttc
     6841 gacttcttcg cgtttggcaa gcaggacgca gcgggcaatg cctttttcgt ggcagatggc
     6901 ggcggcttgg acggtgcggg gttctgcgcc ttcaggcagg acgatgcgtt tgccggcttg
     6961 gcgggcgaag tcgatcaggt tgtagcggaa ttgcgccggc gacaggcgtt ttgcttcgcg
     7021 gcctgccaat acggatacgt ctttcagcgc gtcgctcgaa ccgaagaagg tcaggccggt
     7081 tttctcggct gccgcttcgg caacggaggc tgccgcaccg tccacgacaa aaccttccaa
     7141 tacgcccggc gcggcggcga agaactgttt ggcaaggttc aattggtgtg ccagcgcgtc
     7201 ggcatcggta ttgtcggaac ggacggcgaa gacggctgcc gcgtcaaggg acaatgccag
     7261 ttcgacgttt ttgcctgcga gatagatttt gtcggcatcg ggcgcgatgc cttcgatgac
     7321 gaggttggcg gcatcgagtg cggcaacttt gccgaccagt gcgtcgaacc agtcgtcgct
     7381 tttgccttgc gtgagcaggg tttcggcggt tgcgtcaacg gcttggaaaa cttgtgcgtc
     7441 cagtgctttt gcaaaggctt gtgcggcggc ggaggtgttc agtccggcag atacgggtac
     7501 gacgagtact tttgccataa tatatccttt cgtatgctgc ggtgtgcggc atatgtggtt
     7561 ggaaggggcg gcatatgggc agaaacggct gcctgcgtgc cgtgcgtgcc gtgtttggct
     7621 tggggcgcgc aggttgaata tagcaaacaa attctgtttc caacaagata aatatccgca
     7681 ggcttgtgga tgctgccgcc tttcagaggg tatttccggg gaagaacagg gcgggaccgt
     7741 ccaaatggag gacggcggaa atgccgtctg acagggtggg ggcggaaggg aggttgagcg
     7801 tgaggacggt ttgtccggcc ctgaggctga tttcggtatg ccgcgctttg ggcgtggttt
     7861 tgagaaccac ggcgtgaatg gaggcggcgg gtgcggaatg ggggtgaagg ctgaactgtt
     7921 ccggacggat gagcagtgta ccgcgcgtgc ctgcgggtgc gccgctttgg acgggcaggc
     7981 ggcccaatct gcaatcggcg gtgccgtcgg cgttgagcgc ggcggggaac acgatgcctt
     8041 cgccgataaa cagggcggca tcaaggtcgg caggttgtcg gtacaattcg tgagggcttg
     8101 cggtttggag gatgcgcccc tgtttcatca cggcaatccg gtcggcgtat tgcagggctt
     8161 cttcgcggtc gtggctgaca aaaacggcgg attttccgtt ggcgcgcagg gcggcaatca
     8221 tgtcttcgcg aatctggcgg cgcaactgtt cgtctagcgc gctgaagggt tcgtccaaca
     8281 aaatcagttc ggggtcgggg gcgagggcgc gggcgagggc gacgcgctgt tgttgtccgc
     8341 ccgaaagttc gtgcggatag cgtccggcaa gttcggaaat gccggtcaat tccaacatgg
     8401 cttcgatgcg ctgtcgctct tgcgccgtcc tgcctttgcc gttgccgaga ccgtaggcga
     8461 tattgcggta aacggtcagg tgggggaaca gcacgccttc ctgtacgagg taacccaaac
     8521 ggcgttcgcg gacgggaagg ttggtatttt tcgagaagat ggttttgccg gaaagcgaaa
     8581 tttcgccgga atcgggttgt tcgaaaccgg caaggcagcg taaaagggtg gttttgccgc
     8641 agccggacgc gccgatgata aagagaattt cgcccgggtc gaggctgagc gaaatgtcgt
     8701 ttaaaaccgg ggtgttttga aaacttttgg acaggtgtcc gatgtgcagg gcggcggtca
     8761 tagcggtgct tcctcaagct gttatttgaa ggcgtatttc ttcagcagga atacgggtat
     8821 gccggaaaat aataccagca tcagcgcgta aggggcggcg gcggcgtatt gtgcgtccga
     8881 tgtgtattcc caaacggcgg tggagagcgt gtggacatcg tcggcggtca gcagcagggt
     8941 ggcggtcaac tctttcatca gcttgaggaa gacgagtgcg aatgcggcgg taatgccggg
     9001 caggatggac ggcagtacca acgtcctgaa aataaagaag tgtccgcgcc ccaatgttgc
     9061 gccgacctgt tccatccctt tggggagttg ttccaaggaa gtcctcaggg tggtttgcgc
     9121 catcggcagg taaagcatga aataggcaag gatgacgacg ataaaggttt ggtaaacggc
     9181 aggggtgtag ttgatgctga aataaaccaa ggatagggcg ataaccaaac cggggacggc
     9241 gtgcagtaaa aacggcagcc tgtctatcca aacggttaaa aaattgcgat agcgaaccga
     9301 tgcccaaaca aggggcaagg cacataatat agtcaaaatc gcacctaaag ccgatacgct
     9361 taaggaacgg ataaaggcat caaatacgga tacgagcgcg aatgtgccgg aagtgccgac
     9421 catcatccaa tgtatcaata cgccaaaggg gataataatg cccaaaatca acaagctgct
     9481 taaaaaaaca atcgcgccga tctgaccggg cagtttgagg gttttgacgg gataaggacg
     9541 ggcaacgcct ttgccgctgt ggtaaatctt ggctttgccg cgaaatatgc tttctccaaa
     9601 tacgacgatg ccgcacaccg ccgtcaaaac agcggaaagc agggcggcgg tattgttgtt
     9661 gtaggacatt tcgtattctt ggaaaatggc agtggtaaaa gtggggtagt tcaaaatgga
     9721 taccgcgcca aattcgacca gcatatgcag ggcgatcagt aacacgctgc tgccgatggc
     9781 gggtttgagc tgcgggagga tggcggaaaa aaaggtttgc aggcggcttt tgcccaagga
     9841 caggctgact tcttcgtaag acaggctgat gcgtttgagt gccgcctcga cgggcaggta
     9901 ggcgagcgga aacgaggaca ggctcataat catcactgtc ccccaaaagc cttcgacgcg
     9961 gaaggtcagg ctgatccagg tgaaacagct gacaaatgcg gggatgcaca aaggcagggt
    10021 gattgccgtc tgaaaaaagg ttttgccgaa gaagcggtaa cgttggaaca aaagggcgca
    10081 ggcaatgccc aaaacaatgg aaatcagggt aacgcccgcc atcatcgtca aggtgttgga
    10141 gagcaaatcc cacatacgcg ggcggaacaa cagttcgacg gcgcggttga tgccgacctg
    10201 ccacgaacgc atagcgacat ataaaaaagg cagggtaagc ggcagggcga tcagtaggat
    10261 gaggccggta agccaaatgg gtattttttt aggagacata gtgtttttca tcggcaaaac
    10321 gggcggacag tataaatgtc cgcccgtttg acaatccgaa aacggcttat ttcataccgg
    10381 cttgctcaag cagccgggtg gcgtgttctt tttcggaaac agtggtggcg gacacttggg
    10441 gtgcttccaa cttggcgatg ggttccaaat tgaaggtgga taccacgtgc ggattcaaag
    10501 gatattcggc acggacggcg gtcagggcgc gctgtccttc cttgccggcg aggaaggcga
    10561 cgaatttttt cgcctcatcc ttgttttggg aggattttaa cacggctgcg ccggaatagg
    10621 taacgagtgc gccgggatct ctgtggcgga cgaaattcag gcgggtgtgg acattttgta
    10681 cgcctttttc acgcgcgaaa gcgtgccagt agtagttgtt gatgagggcg gcatcgattt
    10741 cgccgttttc aaccgcttga agggcgacgg agtttttagc gtaaggcttg ccgtattctt
    10801 tcagaccttt gagccatttc aatgcggccg cttcgccttt cagtttgacg atggcgacaa
    10861 tctgttccaa gaacgcgccg gaagtgggga cgtaaccgat gcggtttttc catttcggcg
    10921 tggcgtaatt caggacggat ttttccaaat ctttttcaga cagtttgcgg gtgtcgtaaa
    10981 cgacgacgcg cgaacgtccg ctcagtgcca cccagtcttt tttggcggca accggcacgc
    11041 ctttgccgcg tgtttcgttg atggtggagg cgggcagggg ctctaggagg ttggctgcgg
    11101 aaagggtggc gagtgccggg atttgttcgg aatagaatac gtcggcgggg cttcggctgc
    11161 cttcttcttt gatttggccg gcaagctggt cgcctttggc actgttgagt ttgactttga
    11221 tgccggtagc ccgggtaaag gcatctgcaa cggcttgtgc cgcttctttg tgttggccgt
    11281 tgtacacggt aatgtctgcc agcgcggggg tggcggcggt cagggctgcg gcaagcagtg
    11341 cgtatcggat agatgttttc atatcgattt tctcctaaat gaatgagggt gtataccttg
    11401 ttaagacata acggggtgta gtgtattcct tcttttttat aaatgcaaat aattattttt
    11461 taaatttgtt attatccgat ccggttattg tttgttctga cttgtatttt ttccgtgcat
    11521 cgcgcccgta aggcggaagc ggcgggcaat gtgtggcgga atgtgggtaa aggcggcatt
    11581 ttgatttgtc ggaatgctgg agaacctctc cctttaaaac gccgtctgaa caaggttgcc
    11641 ggaatagtat tgccatcccg gcagatacag tttgtcggga tcttgccaat attgttcatc
    11701 cagactgttc ggcagcgagg cggttttgtt gtcgagatgt tttgttaatc cactatatat
    11761 cccacatttc ttttaggttt ttaccttccg attggaggtg gcggatttct tttcccaatg
    11821 cgtctgccgc ccgatggatc agcattgcgc tgtgtaccgc gccgattttt ttcagaacgg
    11881 aacaagtctt ttcggcggcg ggaaaacccc agttgcagac aaattgcagt atgccgccgt
    11941 tgccgatgtc ggcttccgcc cgccaaatca aaaccagctc ctgttcccgt ccgtccatgc
    12001 tttcgagttt gccgtcatgc tgttcaaaaa gtttgtccac cgcctgccgc atcatctgct
    12061 cgaagcggtc ggcttgggta tccgtatcgg tcatagtatt cttgcctttt aaaaatgccg
    12121 tctgaacatt tcttcagacg gcatttgggg gttaagctga catttcccgc cagcgtttga
    12181 cttggaggcg tacctgttcg ggcgcggtgc cgcccaagtg gttgcgcgcg ttgaggctgc
    12241 cttcgggtgt cagaacgccg tacacgtcgt ctgaaatcag cttgctgaag ccttgcaggg
    12301 cttcgagcgg caattcgctc aaatcaacgc cggcttcatc ggcgtggcgc acggcttggg
    12361 caacgacttc gtgggcatcg cggaagggca tacctttttt gaccagataa tccgccaagt
    12421 cggtggcggt ggcaaaaccc tgcatcacgg cggcgcgcat attgccgggt ttgacggtta
    12481 cgccgcgcat catatcggcg taaatccgca gcgtgtcgat gagcgtgtcg gcggtgtcaa
    12541 acagcggttc tttgtcttcc tgattgtctt tgttgtatgc caagggctgc gatttcatca
    12601 gggtaatcag gccgataagg tgtccgatga ctcggccgga tttaccgcgc acgagttcgg
    12661 gtacgtcggg gtttttcttt tgcggcatga tggacgaacc cgtacagaag cggtcggcga
    12721 tgtcgataaa gccgaaacgc ggactcatcc acaaaatcaa ttcttcagac aggcggctca
    12781 agtgaaccat aaccagcgag gcggcggcgg tgaactcaat ggcaaaatcg cggtcggata
    12841 cggcatcgag cgagttctgg cagatttgtt cgaaacccaa caattcggcg gtgatttcgc
    12901 gctgaatcgg ataggtcgta ccggcaaggg cggcagcgcc gagcggcata cggttgacgc
    12961 gtttgcgaca gtcggccatg cgttcgaaat cgcgtccgag catttcgacg taggcgagca
    13021 tgtggtgtcc gaagctgacc ggctgggcga cttgcagatg ggtaaaaccg ggcatgacgg
    13081 tttcggcgtt ttgttccgcc aaatccaata atgccgtctg aaggttttga atcaggtttt
    13141 ggataacggt aatctggtcg cgcagccaaa ggcggatgtc ggtggcgact tggtcgttgc
    13201 ggctgcggcc ggtgtgcagg cgtttgcccg cgtcgccgat tttgtcggtc aggcggcgtt
    13261 cgatgttcat gtggacatct tccaaatcga gcggccattc gattttgccg ctgcggattt
    13321 cttcgaggat ttccgacatg ccccgacgga tgtcggtcag atcgttttca ctcagcacac
    13381 ccgaccgggt cagcatctgc gcgtgggcga gcgagccttg gatgtcccat tcggcaaggc
    13441 gttggtcgaa accgatggag gcggtgtatt gtttgacgag ttcggaaacg ggttcgttga
    13501 aacgtccgga ccaggttttg tcgtgcataa ggattccttg atggggttat tcggtgcggt
    13561 atttttccaa aaggcggcgg aagggttcgc cggtttcggg gtgttttaaa ccgtaggcga
    13621 cggtggcttc gagatagccg agtttgctgc cgcagtcgta gcgcgtgcct tcaaaggggt
    13681 gcgccaagac aaattcgtga tcgagcagct tggcgatgcc gtctgtaagc tggatttcgt
    13741 tgcccgcgcc gcgcggcagg ccggttaaaa ggtcgaaaat gcgcggggtg aggatgtagc
    13801 gtccgacaac ggcaaggttg gagggcgcgt cttcgggctt gggtttttcg acaatgccgg
    13861 taatgcgttg gaactgtttg agctgttcgg tttcgacgat gccgtatgag ccggtttgcg
    13921 acggttcgac ggtttctacg cccaaaatgc tgttaccgct gcgcccgtac acttcgacca
    13981 tttgtttgag cgcgcctttg ggcgcatcga ttaagtcgtc ggcaaggatg acggcgaagg
    14041 gttcgtcgcc gatggcggcg cgggcgcaca agacggcgtg tcccaatccc agtgcttccg
    14101 cctgacggat gtagaggcag gtaatgttcg gcggcaggat gttgcggacg tgttccaaca
    14161 atttgtcttt atggcgcatt tccaacttag tttcgagttc gtatgccttg tcgaaatggt
    14221 cttcgatgct gcgcttgttg cgcccggtaa caaacaccat ttccgtgcag ccggcttcca
    14281 cggcttcttc tacggcgtat tggatcagcg gcttgtcgac gatgggcagc atttctttcg
    14341 ggttggcctt ggtggcgggc aggaagcggg ttcccatccc tgcaacgggg aagacggctt
    14401 tttttatcgg tttcattctt tttcctttgt atggttttga tgtttaaagg gcgagtttgc
    14461 ggattaaatc ggcgagtgcc tgcgcgcggt ggctttcgcg gtttttgacc tccgaatcca
    14521 attcggcggc ggttttgccg tgttcgggca gataaaaata cgggtcgtaa ccgaaaccgt
    14581 tttgcccgag cggcgtgtcg tgccattgtc cgtgccatat gccctcggcg ataatcgggc
    14641 gcgggtcgtc tttatgacgg acaaaaacca atacgcagac ataagaacaa cttttgtcgg
    14701 ccttgccggc aagttcggcg gcaagtttca ggttgttggc ggtatcggat ttgggattgc
    14761 tgccggcgta acgtgcggaa tggatgcccg gcgcgccgtt tagggcggcg gcacagatgc
    14821 cgctgtcgtc ggcgagtgcg ggcagcccgc tgtgtccggc ggcgtgccgg gcttttgcca
    14881 gcgcgttttc gacaaaggtg ggatagggtt cggggcattc gggtatgccg aatggggatt
    14941 gcggcaatac ggtgatgccg taaggtttga ataagttgcc gaattctttg agcttgccgg
    15001 cattgccgct tgccaaaacg attttttccg gtttttcaga catagcggtt tccctgtgat
    15061 gaagatgggg cggcgcgtag ggatttgtgc cgcaggtaga gggcgagtgc gccgatttgc
    15121 ccgaacagcg cgccgaatgc gaaaaggaag gacgcgacgg cgaaggcttt gctgccggcg
    15181 gtcagcagat aagcacccaa gaggacgggc agcataaatg ccagtgtgaa caaggcaaca
    15241 gacaacagat agatttttcg gcggttcatg gcgttcggtc ggaaacggga tgttcggatt
    15301 atagccgatt aggacggcat tccctagagg ctggaaaaat gccgtctgaa gcggcgttca
    15361 gacggcatgg cgggcattat gcctgtttgt tccaacgttc gatggattct ttgatgactt
    15421 ttttcgcttc ttccgcaccg ccccaggatt cgactttggt cgtacccgct tttttcaggt
    15481 ctttgtagtg gttgaagtgg aactcgattt gtttgatgag ctgttgcggc aaatcggaca
    15541 aagttttgta ggcgttgccg ttattgcggt cgtcggcagg tacgcagacg attttgtcgt
    15601 ccacttcgcc gtcgtcgacg aatttcatca cgccgataac gcgcgcttcc aagaatacgc
    15661 cggttgccag aggttgttcg gtaacgagca acacgtccaa ttcatcgccg tcttcgtcca
    15721 aagtttgggg gatgaagccg tagttggtcg gtttggcgaa gatggcgggt tcgatgcggt
    15781 cgagttggaa tgcggcgagt ttgcggttcc actcgatttt gtggttgctg ccggcgggga
    15841 tttcgttgac gacgttgatg atgccgccgt ccacgtcgcc cggggtcagg atttggttga
    15901 agtctgccat ttggtttcct ttatttggga aggtttgaag tttgaaagta tagcacaaac
    15961 gtccggctgg aaatgcgtcc gatgccgtct gaaagggaaa ccgggcgcgt gttaccgttt
    16021 gcccaaaaac ctgcccagtt ccaaaatcgc gcgcctgttg gacggggaaa acactttttc
    16081 cgccgcttct tccaagccga accagccgta ggagacgtgt tcctcgggtt gcaggacgac
    16141 gggcgtatcg cgcgggattt cggcccggaa gacgtgttcg cggttttcaa acacgccttt
    16201 ggggtagcgg tgccgccagt ggtggtagat ttcgtaaacc gtgctgtcgt gccggtcttg
    16261 gagctgcccg tcctccagca ggatgccggt ttcttcccaa acttcgcgcc ttgccgtttg
    16321 ggcgacggtt tcgcccggtt cgaggctgcc ggttaccgac tgccaaaatc ctttcggatg
    16381 cgtgcgttcg atgagcagga tgccgccgtc cccgctataa aggacgacca gtgcggaaac
    16441 ggggtatttg agcggttttg ccatcggcat ctttcggcgg gctgcggtaa tgaaggggcg
    16501 gattatagca aacgccgcac gttatggcgt ttatcctttt ccgtatcctt ttttctgcac
    16561 ggatgggacg cgccggtgtt tgccggtaaa ttttcctttg tgtcaaaaag ataagggcgg
    16621 ttgtgatttt aatgcttgcc aaagtgacgg gcggaaacta taatccgcga cttaccgagt
    16681 cggagtgtgg cgcagtctgg tagcgcactt gcatggggtg caaggggtcg aaggttcgaa
    16741 tcctttcact ccgaccaaaa attccgaaag ccgctttcaa aagcggcttt tttgccgtcc
    16801 gtatgattat gatgtagagt acgcggcgac agacattcaa atgccgtccg aaaaccgttc
    16861 agacggcatc tctttatctt aatagtggat taacaaaaat caggacaagg cgacgaagcc
    16921 gcagacagta caaatagtac ggcaaggcga ggcaacgccg tactggttta aatttaatcc
    16981 actatagttt cattccgtac catcttaagg aacatcaaat tgggcatttc ccgcaaaata
    17041 tcccttattc tgtccatact ggcagtgtgc ctgccgatgc atgcacacgc ctcagatttg
    17101 gcaaacgatc cctttatccg gcaggttctc gaccgtcagc atttcgaacc cgacgggaaa
    17161 taccacctat tcggcagcag gggggagctt gccgagcgca gcggccatat cggattggga
    17221 aacatacaaa gccatcagtt gggcaacctg atgattcaac aggcggccat taaaggaaat
    17281 atcggctaca ttgtccgctt ttccgatcac gggcacgaag tccattcccc cttcgacaac
    17341 catgcctcac attccgattc tgacgaagcc ggtagtcccg ttgacggatt cagcctttac
    17401 cgcatccatt gggacggata cgaacaccat cccgccgacg gctatgacgg gccacagggc
    17461 ggcggctatc ccgctcccaa aggcgcgagg gatatataca gctacgacat aaaaggcgtt
    17521 gcccaaaata tccgcctcaa cctgaccgac aaccgcagca ccggacaacg gcttgccgac
    17581 cgtttccaca atgccggcgc tatgctgacg caaggagtag gcgacggatt caaacgcgcc
    17641 acccgataca gccccgagct ggacagatcg ggcaatgccg ccgaagcctt caacggcact
    17701 gcagatatcg tcaaaaacat catcggcgcg gcaggagaaa ttgtcggcgc aggcgatgcc
    17761 gtgcagggta taagcgaagg ctcaaacatt gctgtcatgc acggcttggg tctgctttcc
    17821 accgaaaaca agatggcgcg catcaacgat ttggcagata tggcgcaact caaagactat
    17881 gccgcagcag ccatccgcga ttgggcagtc caaaacccca atgccgcaca aggcatagaa
    17941 gccgtcagca atatctttat ggcagccatc cccgtcaaag ggattggagc tgtccgggga
    18001 aaatacggct tgggcggcat cacggcacat cctgtcaagc ggtcgcagat gggcgcgatc
    18061 gcattgccga aagggaaatc cgccgtcagc gacaattttg ccgatgcggc atacgccaaa
    18121 tacccgtccc cttaccattc ccgaaatatc cgttcaaact tggagcagcg ttacggcaaa
    18181 gaaaacatca cctcctcaac cgtgccgccg tcaaacggca aaaatgtcaa actggcagac
    18241 caacgccacc cgaagacagg cgtaccgttt gacggtaaag ggtttccgaa ttttgagaag
    18301 cacgtgaaat atgatacgaa gctcgatatt caagaattat cggggggcgg tatacctaag
    18361 gctaagcctg tgtttgatgc gaaaccgaga tgggaggttg ataggaagct taataaattg
    18421 acaactcgtg agcaggtgga gaaaaatgtt caggaaacga gaagaaggag tcagagtagt
    18481 cagtttaaag cccatgcgca acgagaatgg gaaaataaaa cagggttaga ttttaatcat
    18541 tttataggtg gtgatatcaa taagaaaggc acagtaacag gagggcatag tctaacccgt
    18601 ggtgatgtac gggtgataca acaaacctcg gcacctgata aacatggggt ttatcaagcg
    18661 acagtggaaa ttaaaaagcc tgatggaagt tgggaggtga aaacgaaaaa aggtgggaaa
    18721 gtgatgacca agcacaccat gttcccaaaa gattgggatg aggctagaat tagggctgaa
    18781 gttacttcgg cttgggaaag tagaataatg cttaaggata ataaatggca gggtacaagt
    18841 aaatcgggta ttaaaataga aggatttacc gaacctaata gaacagcata tcccatttat
    18901 gaatagtaat atttatgaaa aattaggaga ttaatgatga aaagaattaa gtgcttttgt
    18961 gataaatttc catcaggaga tacatttaga atgt
//
LOCUS       DAXFZR010000042        18917 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000042 DAXFZR010000000
VERSION     DAXFZR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18917
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.042"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            307..597
                     /locus_tag="ACFJAS_001246"
     CDS             307..597
                     /locus_tag="ACFJAS_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC3982497.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKAGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            690..2324
                     /gene="groL"
                     /locus_tag="ACFJAS_001247"
     CDS             690..2324
                     /gene="groL"
                     /locus_tag="ACFJAS_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC3982498.1"
                     /translation="MAAKDVQFGNEVRQKMVNGANILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            2981..4516
                     /locus_tag="ACFJAS_001248"
     CDS             2981..4516
                     /locus_tag="ACFJAS_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC3982499.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            4513..4605
                     /locus_tag="ACFJAS_001249"
     CDS             4513..4605
                     /locus_tag="ACFJAS_001249"
                     /inference="COORDINATES: protein motif:HMM:NF028261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC3982500.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(4641..5081)
                     /locus_tag="ACFJAS_001250"
     CDS             complement(4641..5081)
                     /locus_tag="ACFJAS_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982501.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRGTGISSERKLWMRRARWNANGSRKTNAV"
     gene            complement(5275..6495)
                     /gene="lysA"
                     /locus_tag="ACFJAS_001251"
     CDS             complement(5275..6495)
                     /gene="lysA"
                     /locus_tag="ACFJAS_001251"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC3982502.1"
                     /translation="MTLFCEQVPYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(6506..6676)
                     /locus_tag="ACFJAS_001252"
     CDS             complement(6506..6676)
                     /locus_tag="ACFJAS_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC3982503.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            6747..7070
                     /gene="cyaY"
                     /locus_tag="ACFJAS_001253"
     CDS             6747..7070
                     /gene="cyaY"
                     /locus_tag="ACFJAS_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC3982504.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            7103..7522
                     /locus_tag="ACFJAS_001254"
     CDS             7103..7522
                     /locus_tag="ACFJAS_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC3982505.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            7544..8050
                     /gene="luxS"
                     /locus_tag="ACFJAS_001255"
     CDS             7544..8050
                     /gene="luxS"
                     /locus_tag="ACFJAS_001255"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC3982506.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            8195..10987
                     /gene="polA"
                     /locus_tag="ACFJAS_001256"
     CDS             8195..10987
                     /gene="polA"
                     /locus_tag="ACFJAS_001256"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC3982507.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            11549..12244
                     /locus_tag="ACFJAS_001257"
     CDS             11549..12244
                     /locus_tag="ACFJAS_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC3982508.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
     gene            complement(12236..12346)
                     /locus_tag="ACFJAS_001258"
                     /pseudo
     CDS             complement(12236..12346)
                     /locus_tag="ACFJAS_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12484..16878)
                     /gene="app"
                     /locus_tag="ACFJAS_001259"
     CDS             complement(12484..16878)
                     /gene="app"
                     /locus_tag="ACFJAS_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC3982509.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRDATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTAFPRARRARRDLPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQD
                     ELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFS
                     HNRTGNTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGKIRRR
                     VLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKA
                     DYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGF
                     TLSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            17316..18662
                     /gene="mnmE"
                     /locus_tag="ACFJAS_001260"
     CDS             17316..18662
                     /gene="mnmE"
                     /locus_tag="ACFJAS_001260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC3982510.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
BASE COUNT         4298 a         4891 c         5206 g         4522 t
ORIGIN      
        1 caggacaagg cggcgaagcc gcaggcagta cagatagtac ggaaccgatt cacttggtgc
       61 ttcagcacct tagagaatcg ttctctttga gccaaggcgg ggcaacgccg taccggttta
      121 aatttagtcc actataaaaa acggcggaaa taaatttttt tccgcctcac ttgaattttc
      181 ccgcacacac cctaattttg ccgacttgta cgggcaggcc gttaggcggc agcccggttt
      241 ccacttcaat ttgtccgaac cgttcgggca gatgattgtt ttcaaaccat ttatcggagc
      301 ataaatatga ccatccgtcc tttacacgac cgcgtcgtcg tcaaacgctt ggaagctgaa
      361 gaaaaaaccg catcgggcat cgtcctgccg ggtgcggccg ccgaaaaacc cgatatgggc
      421 gaagtcatcg ccgtgggcgc gggcaaaatc ggcaaagacg gcgcgcgccg tccgctggat
      481 gtcaaagccg gcgacaaaat cattttcggc aaatacagcg gccaaaccgt aaaagccgac
      541 ggcgaagagc tgttggtaat gcgcgaagaa gatattttcg gcatcgttga aaaataaata
      601 cggacacgat gccgtctgaa acggcaaacc gccttcagac ggcataaacg gttttatcag
      661 acagttttaa tgatttttgg agaattgaaa tggcagcaaa agacgtacaa ttcggcaatg
      721 aagtccgcca aaaaatggta aacggcgcga acattctggc aaacgccgtg cgcgtaacct
      781 tgggccccaa aggccgcaac gtagtggttg accgcgcttt cggcggcccg cacatcacca
      841 aagacggcgt taccgtcgcc aaagaaatcg aactgaaaga caagtttgaa aatatgggcg
      901 cgcaaatggt gaaagaagtc gcgtccaaaa ccaacgacgt agccggcgac ggtacgacta
      961 ccgccaccgt attggcacaa tccatcgttg ccgaaggcat gaaatacgtt accgccggca
     1021 tgaacccgac cgatctgaaa cgcggcatcg acaaagccgt tgccgctttg gttgaagagc
     1081 tgaaaaacat cgccaaacct tgcgatactt ccaaagaaat cgcccaagtc ggctcgattt
     1141 ccgccaactc cgacgaacaa gtcggcgcga ttatcgccga agcgatggaa aaagtcggca
     1201 aagaaggcgt gattaccgtt gaagacggca aatctttgga aaacgagctg gacgtggttg
     1261 aaggtatgca gttcgaccgc ggctacctgt ccccttactt tatcaacgac gcggaaaaac
     1321 aaatcgccgg tctggacaat ccgtttgttt tgctgttcga caaaaaaatc agcaacatcc
     1381 gcgacctgct gcccgtgttg gaacaagtgg cgaaagccag ccgcccgctg ttgattatcg
     1441 ctgaagacgt agaaggcgaa gccttggcga ctttggtcgt gaacaacatc cgcggcatcc
     1501 tgaaaaccgt tgccgtcaaa gcccccggct tcggcgaccg ccgcaaagcg atgctgcaag
     1561 acatcgccat cctgaccggc ggcgtagtga tttccgaaga agtcggcctg tctttggaaa
     1621 aagcgacttt ggacgacttg ggtcaagcca aacgcatcga aatcggtaaa gaaaacacta
     1681 ccgtcatcga cggcttcggc gacgcagccc aaatcgaagc gcgtgttgcc gaaatccgcc
     1741 aacaaatcga aaccgcgacc agcgattacg acaaagaaaa actgcaagag cgcgttgcca
     1801 aactggcagg aggcgtggca gtgatcaaag tcggcgcggc gaccgaagtc gaaatgaaag
     1861 agaaaaaaga ccgcgtggaa gacgcgctgc acgctacccg cgcagccgtt gaagaaggcg
     1921 tggttgcagg cggcggcgta gccctgttgc gcgcccgcgc cgctttggaa aacctgcaca
     1981 ccggcaatgc cgaccaagac gcaggcgtac aaatcgtatt gcgcgccgtt gagtctccgc
     2041 tgcgccaaat cgttgccaac gcaggcggcg aacccagcgt ggtggtgaac aaagtgttgg
     2101 aaggcaaagg caactacggt tacaacgcag gctccggcga atacggcgac atgatcggaa
     2161 tgggcgtact cgaccctgcc aaagtaaccc gttccgcgct gcaacacgcc gcgtctatcg
     2221 ccggtctgat gctgacgacc gactgcatga ttgccgaaat ccctgaagaa aaaccggctg
     2281 tgcccgatat ggggggaatg ggcggtatgg gcggcatgat gtaagcaatg ccgtctgaaa
     2341 ccttcagaca acaagccgca cggtcaacgc cgtgcggttt ttttgcgaat aagtgcggct
     2401 aaggcgcaaa ccttaaaatt atagcggatt aacaaaaacc ggtacggcgt tgccccgccc
     2461 cggctcaaag ggaacgattc cctaaggcgc ccaagcaccg ggcgaaccgg ttccgtacca
     2521 ttcgtactgc ctgcggctgg ccgccttgtc ctgatttttg ttaatccgct atatttcccg
     2581 ccatcccaaa aacgaagagc ggcaggaatt tatcggaaaa acagcacctc tccgccgtca
     2641 ttcccgcgaa agcgggaatc taggttcgtc cggtttcggt tatttccgat agattcctgc
     2701 cgcgttgggg gtctggattc ccgcctgcgc gggaatgacg gggaagttgg cggttgtttc
     2761 ttatggagtg gtttttgtat ttggataaaa aatttttcgt tttcaagcct tcaccgcttg
     2821 ccatcggcgt taaaattttt tacgataagc acatagattg taaacaatcg gccacaagcc
     2881 ggtttgtttt ttcagaagac attatccctg tcagacgctg tttctatata tgtttgccta
     2941 taacggcttg tttttaataa ataattcaag aggtatcaac gtgtctgatt ccaaaacgaa
     3001 agaacgcgcc acattcggca cgcgccgcgc gtttatgatt gccgccatcg ggtccgccgt
     3061 cggcttgggc aatatttggc gtttccccta tattgctttt gaaaacggcg gcggcgcgtt
     3121 catcctgccc tatctggtcg cgcttctgac ggcgggcatc ccgctgctgc tgctcgatta
     3181 tgccatcggc caccgttacc ggggttctgc gcccttggct ttccgccgcc tcggacgctg
     3241 gttcgagccg gtcggctggt ggaacgtgat gaccaatatc gtcatctgca tctattacgc
     3301 agtgattatc ggttgggcgg caagctatac ctattattcg gtcaacgccg cctggggtgc
     3361 ggatccgcag ggttttttct ttaaggactt cctgcaaatg gcgggcccgg aagccttggg
     3421 tttggatttt gtcggcaaag tcgccggccc tttggcgggc gtgtgggttt ttaccgccgc
     3481 cattatggct ttgggcgtgc aaaagggcgt ggcgcgcgcc tcgtcgttct ttatgccgct
     3541 gcttttggtg atgtttttga ttatggtcgg catttcgctg acgctgccgg gtgcggcaaa
     3601 gggcttggac gcattgttta cgcccgactg gtcgaaactc gccgattcca aggtctgggt
     3661 ggcggcatac gggcagattt tcttttcgct ttccatctgc ttcggcatta tggttaccta
     3721 ttcttcttat ttgaagaaaa aaaccgactt gggcggaacg gggctggtgg tcggttttgc
     3781 caacagcagc ttcgaactgc tcgcgggcat cggcgtgttt gccgcattgg gctttatggc
     3841 gcaggcgggc ggcaaggcgg tcaacgaggt tgcctccggc ggcatcggtt tggcgtttat
     3901 cgcctttccg accattatca accaggcgcc gatgggctgg ctgatcggca tattgttttt
     3961 cggttcgctg gtgttcgccg gcgttacgtc gatgatttcc atccttgaag tgattgtggc
     4021 ggcgattcag gacaagctga acatcgggcg cgtcaacgcc acgctgctgg tctgcattcc
     4081 gatgggcatt gtttccacgc tgctgttcgg tacggcgacg gggctgcctg ttttggacgt
     4141 gatggacaaa ttcgtcaaca cctacggcat tgttgccgcc ggctttgttt atgttgccgc
     4201 catcatcatc ggcggcaggt tgccggaatt acgcaggcac ctgaacgcct tgtcctccat
     4261 ccgcgtcggc ggcttgtgga cggcctgcgt cgtgtttacc gtcgtgatgc tcggctatat
     4321 gctgtatcag gacaccgccg gattgttgga aaaaaactac ggcgattatc cggatggttt
     4381 cctcaatatt ttcggctggg ggatgtcggc ggcgttaatc atgttcgggc tgctgctgtc
     4441 gctgctgcct tggaaacacg gtcaggattt taatgtcaaa gacgaacacg aacatgaaca
     4501 aggaggagaa aaatgagtac ttccgccatt gtgatgatgg ttgccgccat cgcggtgatt
     4561 tggggcgggc tgctgctttc cctgttaagg ctgccggaag agtaagcctt tagaacgtta
     4621 aaaatgccgt ctgaaccgct tcagacggca tttgttttac ggctgccatt cgcgttccac
     4681 cttgcccgac gcatccaaag tttgcgctcg gacgatatgc ccgtcccccg gtacaggatg
     4741 cgcgtccgca atatgccctg ttcggtatag ccttgcgcca cgccgttttc aaccggcgtg
     4801 tgcgaatgca gtttgccgtt tggatggtaa atatcgacaa agcggtcgaa cacgccgttt
     4861 ttgacgatgc ttttcaagac gcgcccgttt gaggcgcggg tgctgaatgc gccgtccggc
     4921 gtgcctgaag gcggccggct gtatgggttg gcgggttgga tgggcgcgct tttcggcagg
     4981 gtgcgaagcg gcggccggtc agagggaagg gcggtgcagc ccgccgaaag tagggcggcg
     5041 gacgaagcgg taagcaggag gataatcgga gcgtgcttca tttcaattcc ttagatattg
     5101 caccccataa aaggggcgcg taattttttt caacaattcc cttcgtcagt cgggcgagac
     5161 ggggatttgt tgcgtcgtca tttgaaaaca ggctgtgttt gaatattccg ccaagatgtc
     5221 actttaaacg gacaatgccg tctgaaggct tcagacggca ttttgatgtt cggcttatag
     5281 gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg cgccggcgga tgagtttgta
     5341 tccgccgccg tcaaccaaca cctccgccgc acggttgcgc gtgttgtaat tgctagccat
     5401 actggccccg tatgcgcccg cgctgcggat aagcagcaaa tcgccttctt cgcaggcgat
     5461 ggtgcggtct ttgccgagga agtcgccggt ttcacaaatc gggccgacga tgttggcggt
     5521 cagaggctca atgtttttgg tttcaaccgc ttcgatgtgg tggtaggcat cgtatagggc
     5581 tgggcgcatc aaatcgttca tcgccgcatc gaccatcaca aagtttttct cttcaccgtg
     5641 tttgacaaat tcgacgcgcg tcagcaatgc acctgcgttg ccgaccaagc tgcggcctgg
     5701 ctcaagaatg agtttcagac ggcgtgtccc catcagtttt tgaaccgctc gggcatacgc
     5761 acccaaatcg gggacgcctt cgtctttgta aacaatgccg acgccgccgc ctaagtccaa
     5821 atgttccaaa acaatgcctt cggcggcaag agcgtcaacc aaaatcaaaa tgcgttcgca
     5881 ggcttcgacc agtgggctta agtcggtcag ttgcgaaccg atgtggcagt cgatgccgat
     5941 gattttcaaa ttgggctgtt gtgcggcatg gcggtaggct tcgagcgcgt cggcgtaggc
     6001 gatgccgaat ttgttggctt tcagacctgt ggagatgtag ggatgggttt ttgcatcgac
     6061 atcgggattg acgcgcaggg agacgggcgc ggttttgccc aaacgcgcgg caattttctg
     6121 aatgcggtcg atttcgggga tgctttccat attgaagcat tttacgccgg cattcagcgc
     6181 gaactcgatt tccgcctcgc ttttgcctac gccggaaaaa atcgttttcg ccgcatcgcc
     6241 gcctgccgcc aaaacgcgtg ccaattcgcc gcccgacaca atgtcaaaac cgctgcccaa
     6301 cgaggcgaag tgtttgatga tgctcagatt gccattggct ttaaccgcat agcagacgag
     6361 cgggttcaaa gcggcaaagg cggtttgata gttttcaaat gctccggtca gcgcggattg
     6421 gctgtacaca taaagcggcg tgccgaattc ttcggcaagg cgggggtagg ggacttgttc
     6481 gcaaaacagg gtcatatttt cgttttcatt tttgggttgg tggggcggat tgcggtttgc
     6541 cttgaagctg caaaccggtt tggatcacgc cgaaacgcgc cgtatcgcct tctttgggca
     6601 ggtagaggcc gcctttgtaa ccgcaggcgg aaagcaggag ggcggttgcc gccgcaaaaa
     6661 atacgccgta tttcatcggt aaacttcctt cataagcgcg aatgtggcaa gattcggcat
     6721 cttaaacaaa aaacacgcaa aaagctatga tgaccgaaag cgagtttatc cgcgcgagcg
     6781 aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa cggctgggat ttcgactgcc
     6841 ggtttgccgg aaacgtcctg accatcgaag ccggagacgg cacgcaaatc atcgtcaacc
     6901 gccacacgcc caaccaagaa ttgtggattg ccgcaaaaag cggcggctac catttcgccg
     6961 aacaaaacgg caaatggctg gcaacgcgcg acagccgcga tttttacgac gttttaaacg
     7021 aagccctgag cgcggcttcg ggcgaagcgg ttgagattgc cgaattgtga ttcaattttc
     7081 gatataaaaa gattgttacc caatggcaca attacctcta tatcggactg ccgaaatcgg
     7141 aaattttact gtcggcacgc ctaaagtttt agaatcattt tccaaacata tcccttatgg
     7201 tgttgtcttt gaagatgatg gcaacacggg ctacttctat gccgcttcgc aagaggggat
     7261 tttggatgcc ttgcacatct acaatgtcga agatgtatcc gacaaacata tccccaatca
     7321 tgtcttgatt ttatgggatg gcgcctgcac catagccgca ttgtgtatca acgactacat
     7381 ccatgccgtc tatgattttg tcgaacaggc aggatattgc cgcaacggct tccctgaagc
     7441 aggcggcgaa tgggtaaaag tcgaaaaccg cgtcttggac gatgaattgc tggacaaaat
     7501 cctatcccga aaacctacat aacccgcaca aaaggatacc caaatgcccc tgttagacag
     7561 tttcaaagtc gatcacaccc gtatgcacgc ccctgccgta cgcgtggcga aaaccatgac
     7621 cacgcccaaa ggcgacacca ttactgtatt cgacctgcgc ttttgcatcc ccaataaaga
     7681 aatcctgccc gaaaaaggca tccacacgct ggagcatttg tttgccggct ttatgcgcga
     7741 ccacttgaac ggcgccggcg tggaaatcat cgacatttcc ccgatgggct gccgcaccgg
     7801 tttttatatg agccttatcg gcacgccttc cgaacagcag gttgccgatg cgtggttggc
     7861 ttcgatgcag gatgttttga atgtcaaaga ccaaagtaaa atccccgagt tgaacgaata
     7921 ccaatgcggc acttatctga tgcactcgct cgccgaagcg caacaaatcg cgcaaaacgt
     7981 gttggcgcgc aaagtggcgg ttaaccgaaa cggcgatttg gctttggatg aaagcctgct
     8041 gaacgcttaa tccgccgcaa atgctgtctg aacaagggtt tcagacggca tttgcctttt
     8101 ccgttataat ccggggttgt acgggggcgg gttttaagcc ggcatcgtcc ttccctattt
     8161 tttctgtccc ttatcggttt caagcgggtt ttttatgtcc aacagaccta cactcctcct
     8221 cgttgacggc tcttcctatc tctaccgcgc gtatcacgcg atggggcaaa acctgactgc
     8281 gcctgacggt gcgccgacgg gtgcgctgta tggcgtattg aatatgttgc gccgtttgcg
     8341 gtcggaatat ccgcacgatt attgcgcggt ggtttttgat gcgaaaggca aaaatttccg
     8401 ccatcaaatg tttgaagaat acaaggcgac gcgcccgccg atgccggacg atttgcgccc
     8461 gcaggcggaa gccctgccgg atttggtgcg cctgacgggc tggccggtat tggtgattgg
     8521 gcaagtggag gcggacgatg tgatcggcac gctggcgaaa cagggggcgg aacatggttt
     8581 gcgagtcatt gtttcgacgg gcgataagga tatggcgcag ttggtggatg agcgcgttac
     8641 gctggtgaac acgatgagcg gagaaacgct ggatattgaa ggcgtgaagg caaaattcgg
     8701 cgttcgcccc gaccaaatcc gcgattatct cgcgctgatc ggcgacaagg tggacaacgt
     8761 accgggcgtg gaaaaatgcg gcccgaaaac ggcggtgaaa tggctggaag cctacggttc
     8821 gttgcaaggc gtgatggaac acgctccgga aatcaaaggc aaggtcggcg aaaacctgca
     8881 agccgcgctg ccccaactgc cgctgtcgta tgatttggtc acgattaaaa ccgatgtgga
     8941 cttgcacacc gagctttcag acggcatcga aagcctgcgc cgtaccgcgc cgaaatgggc
     9001 gcagcttgcg gttgatttca aacgctgggg cttccgtact tggctgaaag aagcggaatc
     9061 aaacatgaat accggctcga ccgatgattt gttcggtagc gacagcatcg gcgagcaggc
     9121 ggctttgaat gcggaaatac cgtctgaaaa acgagctgaa aaagccaccg cccctgaaaa
     9181 actggattat caagccgtta ccaccgaagc tcagtttgcc gccttgttgg acaaactagc
     9241 gaaggcggac acaatcggca ttgatacgga aaccacgtca ttagatgcga tgaatgccga
     9301 gctggtcggc atcagcattg cgttccaagc aggcgaagcg gtttacatcc ccgtaggcca
     9361 cagcctgact gccgcgcccg aacagcttga tttgcaagac gtattaggcc gtctgaagcc
     9421 gcatttggaa aaccccgccc tgaaaaaaat cggtcaaaac ctcaaatacg accaacacgt
     9481 tttcgccaac tacggcatcg cgttaaacaa cattgccggc gacgcgatgc tcgcttccta
     9541 catcatcgag agccatctcg gacacggctt ggacgaattg tccggacgct ggctcggttt
     9601 agaaaccatt acctacgaat cgctgtgcgg caaaggcgcg aagcaaatca gttttgccga
     9661 tgtcgccatc gggcaggcga ccgaatacgc cgcccaagat gccgatttcg ccctgcgcct
     9721 cgaagcgcgc ctgcgcgcgc aaatggacga caaacaactt gaaatgtatg aaaaaatgga
     9781 gcttcccgtc gcgcaggtat tgtttgaaat ggaatgcaac ggcgtgcaaa tcgaccgcgc
     9841 cgaactcgcc cgccaaagcg cggaactcgg cgcggagctg atgaagctcg aacaggaagc
     9901 ctacgccgct gcaggtcaac cgtttaacct caactcgccc aaacagctgc aagaaatcct
     9961 gttcgacaaa atgggcatcc cgacaaaagg gctgaaaaaa accgccaaag gcggcatttc
    10021 caccaacgaa gccgtgctcg aacagctcgc gcccgactac cccctgccca aaatcatcct
    10081 gcaaaaccgc agcctggcga aactcaaatc cacctacacc gacaaactgc ccgaaatgat
    10141 ttcgccccgg gataaccgcg tgcataccac ctatgcccaa gccgtcgcca ttaccggccg
    10201 cctcgccagc aacaacccca acctgcaaaa catccccatt cgcaccgccg aaggccgccg
    10261 cgtgcgccgc gccttcactg caccgccggg cagcgtcatc gtttccgccg actattccca
    10321 aatcgagctg cgcattatgg cgcacctctc cggtgacaaa accctcattg ccgcatttca
    10381 aagcggcgaa gacgtacacc gccgcaccgc cgccgaagtg ttcggcactg cgcccgaaaa
    10441 cgtctcgccc gagcaacgcc gctacgccaa aaccatcaac ttcggcttaa tctacggtat
    10501 ggggcaatac ggtttggcaa aatcattggg catcgacaac ctttccgcca aaaactttat
    10561 cgaccgctac ttcgcccgtt atcccggcgt cgccgaatac atgcagcgca ccaaagaaca
    10621 agccgccgcc caaggctacg tcgaaaccct gttcggcaga aggctctatt tgcccgacat
    10681 ccgcaacaaa aacgccaacg cccgcgcagg agccgagcgt gccgccatca acgcccccat
    10741 gcaaggcacc gcttccgacc tcatcaaacg cgccatgata aacgtgcgaa actggctttc
    10801 agacggcatc ggcagcaaac tggtcatgca ggtgcatgac gaactggtgc tggaagtcgt
    10861 tgaaaccgaa ctggattttg tcaaagaaaa actgccgcag attatggcga aagtggacgg
    10921 cggattattg gatgtaccgc tggtggctga ggttggcgta ggggagaatt gggaagaggc
    10981 acattgagaa tgcacaagta ttgaactgtt acgttttgat ttatcaaaat ttaaggaagt
    11041 aaaatacccg attcgaatga ttggaaatca atagcaagcc gtaggtcgga tacttgtatc
    11101 cgacaaaagc ctgccatctc aaatagcagt cggattcgag aatccgacct gccaaaccgg
    11161 gcgcggacgc tccggtcggc agttagcgca caaatcgaac agaacatcac aaaaaagcct
    11221 gattcggatt ttccaatcgg gcttttttgc gcccgttttg tcatcccgtg aaatatccgc
    11281 ataacaaaaa taatgcaaaa tcatgacaaa accggcgcga ggttacacaa acggatgaaa
    11341 tcaaccgata ttcaaacaca gccattttta gcgcattttc agcgtatctt tatgcggaaa
    11401 atttcgtgaa caggtttttt gcacaggctt ccgccgcgtt tcgcgggatg ggaaaccgta
    11461 ttagaaaacg gacgcacgca acataaaccc cgaaagtgat gataagatga tgatttaacg
    11521 tactgcttta attatttaag gaattatcgt gtttcccgac aaatacaagt tgagtttgga
    11581 agaaaatatt tttctggcaa agaaagtatt ggttgctcaa attcacaacc tcagccgttt
    11641 tgagaattgt cagacgacct tgttgcagac cgagcaaatt atcaatggca aaaatgtagc
    11701 ctccgcgtca ctggaagaca tccaaaccat cttgaacctg aaacgtgcct atcaatatgt
    11761 gatttcgcat atttcaaacg gcgaaccggt caatatttca ctccttaaaa gaatcaacaa
    11821 cattgttgcc aaggacgatt ctttggtacc cgtggatttc cgtaccggtt cggtcggcgt
    11881 aacgctattg gacggttccc gtcatgcccc gaatccggtg aaggaaattg aagtggcccg
    11941 cgtgttgcaa aatatcggac tgcaaagcgg ttcgacgacg gaagcagccg ttcgtttcat
    12001 gctctattgt atgcggcagc aggttttttg ggacggcaac aaacggacgg caaccctatt
    12061 tgccaacggc ctgatgatgg cggggggctg cggtatcctg gaaatctccg aaatgcagat
    12121 gccgcgattc aatgaaaaac tatccgcatt ctatcgcacc ggcgacgata ccgatatttc
    12181 caagtttgtg tatcaaaatt gtatatcggg catagactat ttcggcgcgg acgaagatat
    12241 atagtggatt aaatttaaac cggtacggcg ttgcctcgcc ttgccgtact atttttactg
    12301 tctgcggctt cgccgccttg tcctgattta aatttaatcc actatagatt tccccgactc
    12361 gccgaacatg ggctaaaaat caatttgacg gttatcagac gatggagtag gcacaaggtg
    12421 gcagaaaaag gggtttgaca gtgcacggcg gcatcgtcaa acccctttcg gcatatccgg
    12481 cggttaccag cggtagccta atttgatgcc cgcgctgtgc tgcgcttcca attgcggccc
    12541 cttggcggcg gcagcgtgga gggacagcgt gaaacctttg atttcggcgt ttacgcccca
    12601 ttccgcactg cgggttttgc cgaaatcctg cgccaatacg gcggtattga cgcgcgttcg
    12661 gactttgccg gaagcggcat cggtatagga caggctcaaa taaggcgtga tggaaatgtg
    12721 ttgcgccggt ttgaatgaat aatctgcctt aatgcccgcg cggtagcggt tgaatgcaag
    12781 gcccggggtg gcgatattga cgttttcgta tcggtaatcc gctttttgga cgaaatagcg
    12841 cgttgcgccg atgtgcggtt cgatgccgaa tccgccgaaa cctgcgcggt atcttgcctg
    12901 aatgccgtaa tgcagcacgc ggcggcggat tttgcctctg atgccgtctg aaaggctgcc
    12961 gctactaaaa cccgcgcccg cgctgatgcc gatgtcgaac ctgccgatgc cgtattgccc
    13021 gaaaacggca ccgtgggcaa gccgtgccga gttgccgatg ccgtcgtcga aggtgtttcc
    13081 ggtccggttg tgcgaaaaca ggatgccgac gcgcccgctg ccgaggtttt tctgcatacc
    13141 gatttggcgc aggtcggttt gttggcggta ggcgcggaaa tcttgcgaac ggtagtgttt
    13201 ggtgtcccgg atgccgcttg tccaaacggc gttgcggcgg tcttcggcaa acacgcggtc
    13261 caattcgtcc tgtacggcga aaacgctgtt gagcgtggcg gaaaattcac tcaaaccgct
    13321 attggcataa cggctgatca ggtcacgctg cggttggggc tgcggttgcg gcaaatcccg
    13381 gcgggcgcgg cgggcgcggg ggaaggcggt ggtagccggc cgggtttccg cttcgcgctg
    13441 tttcgccaag gcggtgtctt tatccgcctg cacccgtttt ttctcttcct ccgcctgcat
    13501 aatgccggca ttttccccgc ctgcctgccg ggccggttcg gcaacgcttt ctgccttttc
    13561 ggtggcatcg cgcccggccg caatcagcgc gtcaaggctt tgcgcgttgt ctttttccgc
    13621 ctgttgtttg gcggcaagtt gtgcctgttt tgccgtcaag gcggcctctg tttctcccgc
    13681 cttgccgagt ttgtcggaaa gctcttgttc tttgaccgga ttatgcaggc ggaactcgcc
    13741 gtctttgcgg ataagctgat aacgccatgc gccggcatcg acgtgttcgt tttgcagggt
    13801 gaaattaaga ttttcggaca gcggtgtgtt gtcttttcct tccactaccg tcaattgctc
    13861 gagacttacg ggttcgttgc cggtattgtt gacagccaag gtgtaagtgc cttcggaact
    13921 ttccgccagc ttcaatttgc cgctgcggta gccgaagagt tccgacataa agcggaatgt
    13981 tccctgaccg ttcaatttgc cgtttaccgt cagcgtgttg aaacgggatt ctgccgaagt
    14041 tggcggcgta acggataata gggaacggcg cgaacggcgg cgcggcgcat ctgccgcact
    14101 gccggtttgc gcgcctgccg catcgtgtcg ataggcggaa ttgagtgtaa tggtggcgtt
    14161 gtcaaggttt aaattgccta attccgtgcc cgacggcagc gtccattcgc tgtcttttaa
    14221 gtgtaatgcc gtatccttgc cgccgctgat ttttccggta aagcggctgt tttcaaaatg
    14281 gaatactgcc ttatcggcta gggagacatt gccgttgagt gcggaatggc ttacgtttgc
    14341 cttagcgttg tcggaaagcg tcagactgcc gttttgtacg gcgttgttgc ttagattaaa
    14401 tgaagcattg tccgaagccg atgtgttgcc gtttaatgtg gcttgattaa atgttgcttg
    14461 ggcattgccc acgaggctga ggttgccgtt ttgggtggcg ttgcgcgtaa ccgtatagtg
    14521 cgtgtctccg cctgcactaa gattgccgtt gagtgtggca agtcctgtga gatttaaatg
    14581 agcgtgatcg gcaaggctga cattgcctct gatgtcggtc ttgctcaatg aagcaatcac
    14641 tttatcgtcg gtaatggttt tttcggtaca acttgtcaga cccgtccagt ccgaacgtgt
    14701 acagattgtg tggctttgat gcggtgcgac accgaaaact gcttgggcgt gattgcttaa
    14761 atgccaatcg ccttccactt tggcaacatt gcgggaaacc accgcttgtc cgccctgaat
    14821 atggaagttt tccgctttaa atgtgcggtc gatccaatcg ttgtcccaca cgatttctcc
    14881 ttgtgggata ccttccattt ttgaccaccc gcttcctaaa tgattgtagg cgtgcggtgt
    14941 cggtctgccg ctgaaaaaca gtttgccgtt tgtttgcgtg atattgccgt ttaaatttgt
    15001 tccgccggaa agcagtaaag tgcgatccgc ttcttccggt tggtaattca gattgagccg
    15061 cccgttcgtt ttggttgcat ctttctcgcc aaaccaaccg ttgtaggcaa tttctttttt
    15121 gctatccaag ttgttgttat tgccggttgt agtaatatct ttattgcctg taatggtaac
    15181 ggtggattct ttgtcttgat tgtggttgac aatcatcgcc ccttcatcgg tattttgaat
    15241 gcggtggaac gaaagcgaat gcccgttcaa atccaaacgt ccgccgcgaa agccgaaata
    15301 gagtttgtcg gggttgaact gattatcggc attcagttgc accgtccccc tgccgctgac
    15361 caagccgatt tcactaaagg cttgtttttt gccttgatcg tccgcctgct gatctaagat
    15421 gactttaccg tcgcccacgc tgaccgagcc ttggttttcc cctttggctt gaaccagcag
    15481 cgtgcctttg ccgattttgg acaggcggtc gtttgccacg ccgtttactt tccaagtaac
    15541 ggtactgcca tcactgatat gaacgcccgc gccttgccac gtttcgttgt ttttaggcga
    15601 gaccgtaaaa ttaccctcaa aatacaaacc gcccgcgcct tggttgatgt tgctggtaag
    15661 tatcaattca ccttttcctt tgtcaataaa ggaaatattt tctccattat tcagtctggg
    15721 tcgataactg ttgaccccac ctgcagcatg ataaacaggt tctcttgctg tctcggataa
    15781 agaaacatta aacaattgaa cggttcgtgt ttttaatcta taaggtagag aatagtgttt
    15841 atgtttggca tcgatttttc ctgcgccatt attattgtcg ttaaaaaagt atttcccatt
    15901 ttgatgtggt tcgtagaata ctgaatgggt atctccagca aagatttcat catagaacca
    15961 atctttacga actagctgga agccattgct ttttcctata taggggttgc ctgtttgcaa
    16021 taccccatta attaaccact tttgcttttg ggcatcatag ataaacattg gtgagccact
    16081 gtcgccaaat gagcctcctg ttggtaaaaa accatatggg ctatgtttaa ttttttcgct
    16141 acctaagttg actgtgccac cacctgatcc attttgtgca aaggtattgc caccgacgag
    16201 ccaagaatat gcgcttgcaa tatgatatga actttcgcgg ttattgggtt cgtcttcatc
    16261 agaccgccaa tattgtctgc ctgctccgat tcgaacacga tcagggtatt tatttaaatc
    16321 agcgtatttc cacccatcca tataactggt catctcaaca ggttctgcat ctgtgacaaa
    16381 tttgtgcaaa cgcggcatat gataatcgcc gccataagga tggccgttag tccctgcttt
    16441 ataattattt cttttcacaa tttggtaaga aaagcggtgc tgatcgggat tgcttccctc
    16501 cgcaccaaaa tcaacattgt tatagccgcc gttatgtgcc acgctcacaa tatattgatc
    16561 gcccgccaat gccgccacgc cgttacgcga taccacagaa aaatcaatca tcggggcttt
    16621 cgtcatcgat ttgccgacca actccccttt tttgttgtaa acctcaatat ctttcgcccc
    16681 gactgcaaac ttgcctttat tttcggcaaa gtcgcgatag tattggtagt tgatgccgaa
    16741 ataagtgtgt cccgcccggg cttggggcag aatgccgaac gacaggcata tggctaagta
    16801 agcgggcgag aagcggatgc ggccggtttt aggggctttg cggtgtgttt cggttgtccg
    16861 tttgtcggtt gttttcatta tttttcctta tctgacggga ttcggtttgt ttgggagggc
    16921 gcggcttccg cttccgggcg gcgcgcggga tgtggctata tgtgcggttc ggcgtatttt
    16981 gccgcttctg ttcggagatg gtgcagggaa gggcgggaag gaaaaaaagg ggatttttat
    17041 caactcgccg gagcaatcga tgggttgcac ataaaggcat cattttgaat acgaaatcat
    17101 atcacacttt actttatccc cgttcctttt cggtcggaac cccgccgttt tcggcggtaa
    17161 aaaccgaccg gaggcggaat gcggggcagg gttttgtgcc gtgcggtgtc ggaatatcgt
    17221 tttacacaag ccggcaatgc catctgaaag ccgaaccgtc ggacttcaga cggcatttgc
    17281 tataatcgcg gctgttttga attttcgggg gttttatgtc ggataacgtt ccaacgattg
    17341 cggcagtcgc gaccgcgccc ggacgcggcg gcgtgggcgt gatacgcata tcggggaaaa
    17401 acctgttgcc gatggcgcag gctttgtgtg ggaaaacgcc cgagccgcgc gtggcaacct
    17461 atgctgattt tacggacgcg gacggacagg caatcgatag cgggctgcta ctgttttttg
    17521 ccgcgccggc aagttttacg ggcgaagatg tcatcgagct tcagggacac ggcgggccgg
    17581 tagtgatgga gatgctgctg aaccgctgtt tggaactggg cgcgcgcctt gccgagccgg
    17641 gcgagtttac caaacgtgcg tttttaaacg acaaactgga cttggcacag gcggaaggcg
    17701 tggcggattt gattgacgca tccggccgtt cggcggcgcg tctggcgttg cgctcgctca
    17761 agggcgattt ttcgcggcgg atacacggtt tggtcgaagg cttgattacc ttgcggatgc
    17821 tggtcgaagc ggcgctggat tttcccgagg aagacatcga ttttctcgaa gcggcggacg
    17881 cacgcggcaa actggacggc ttgcgccgcg ccgtggatga tgtgcttgcc aacgcgcagc
    17941 agggcgcgat tttacgcgaa ggtctgaatg tcgtattggt cggtgcgccg aatgtcggca
    18001 agtccagcct gttgaacgcg ctggcgggcg acgaagtggc gattgttacc gatattgccg
    18061 gaacgacgcg cgacgcggtc agggaacgta tcctgattga cggcgtgccg gtgcatattg
    18121 tcgatacggc aggtttgcgc gagacggacg acgtggtcga acgcatcggc atcgaacgca
    18181 gccgcaaagc cgtatccgaa gccgatgtcg cgctggtgtt ggtcgatccg cgcgagggtt
    18241 tgaatgaaaa gacgcggatg attttggata cgttgccgtc ggatttgaaa cgcattgaaa
    18301 tccacagcaa atccgatttg cacgcacacg cggcaggcgg gttcggtacg ggcgcggaaa
    18361 ccgtcatcgc gctgtcggcg aaaaccggcg acggcttgga cgcgctgaaa cggacgctgt
    18421 tgtgcgaggc cggttggcag ggcgaaagcg aagggctgtt tttggcgcgg acgcggcacg
    18481 tcaacgcact caaggcggcg caggaagaat tgtcgctggc cgctttgtgc ggcaaccatc
    18541 aaatcgagct gtttgccgaa cacttgcggc tggctcaggt cgcgtgcggc gaaattacgg
    18601 gggaatttac ggcggacgac ctgctcggcg tgattttttc gaggttttgc atcggaaaat
    18661 aagcgggccg gaggcattgt ggcggtgtcc ggctgaacat tccgttatcc cataaaaacg
    18721 ggaatccgat ccgttcggtt ttggtttttt gaatttcggg caacgcttaa atcttcattc
    18781 cgcgcaggcg gaaattatcg gcgcggtacg gcaacttttt tcaatactga aagccccgtc
    18841 attcctgtaa aaacaaaaaa atcaaaaaca gaaaactgaa atatcgtcat tcccgcgcag
    18901 gcgggaatcc agccccg
//
LOCUS       DAXFZR010000043        18835 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000043 DAXFZR010000000
VERSION     DAXFZR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18835
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.043"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(141..768)
                     /locus_tag="ACFJAS_001261"
                     /pseudo
     CDS             complement(141..768)
                     /locus_tag="ACFJAS_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
     gene            complement(1033..1248)
                     /gene="rpmE"
                     /locus_tag="ACFJAS_001262"
     CDS             complement(1033..1248)
                     /gene="rpmE"
                     /locus_tag="ACFJAS_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC3982511.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            1383..1880
                     /locus_tag="ACFJAS_001263"
     CDS             1383..1880
                     /locus_tag="ACFJAS_001263"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFC3982512.1"
                     /translation="MESKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLLYSTNHKFDRENKRFEG
                     YTEIRPITLEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKK
                     NLPEG"
     gene            complement(1961..2449)
                     /locus_tag="ACFJAS_001264"
     CDS             complement(1961..2449)
                     /locus_tag="ACFJAS_001264"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC3982513.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVTKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(2446..2829)
                     /locus_tag="ACFJAS_001265"
     CDS             complement(2446..2829)
                     /locus_tag="ACFJAS_001265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC3982514.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            complement(2829..3191)
                     /locus_tag="ACFJAS_001266"
     CDS             complement(2829..3191)
                     /locus_tag="ACFJAS_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC3982515.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            complement(3316..4143)
                     /locus_tag="ACFJAS_001267"
     CDS             complement(3316..4143)
                     /locus_tag="ACFJAS_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC3982516.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            complement(4140..4418)
                     /locus_tag="ACFJAS_001268"
     CDS             complement(4140..4418)
                     /locus_tag="ACFJAS_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC3982517.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            complement(4469..5059)
                     /locus_tag="ACFJAS_001269"
     CDS             complement(4469..5059)
                     /locus_tag="ACFJAS_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC3982518.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            complement(5090..5584)
                     /gene="mlaD"
                     /locus_tag="ACFJAS_001270"
     CDS             complement(5090..5584)
                     /gene="mlaD"
                     /locus_tag="ACFJAS_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC3982519.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            complement(5635..6411)
                     /gene="mlaE"
                     /locus_tag="ACFJAS_001271"
     CDS             complement(5635..6411)
                     /gene="mlaE"
                     /locus_tag="ACFJAS_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC3982520.1"
                     /translation="MNFIRSVGAKTLGLIQSFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            complement(6459..7259)
                     /locus_tag="ACFJAS_001272"
     CDS             complement(6459..7259)
                     /locus_tag="ACFJAS_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3982521.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            7432..8347
                     /locus_tag="ACFJAS_001273"
                     /pseudo
     CDS             7432..8347
                     /locus_tag="ACFJAS_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8455..9886
                     /gene="aldA"
                     /locus_tag="ACFJAS_001274"
                     /pseudo
     CDS             8455..9886
                     /gene="aldA"
                     /locus_tag="ACFJAS_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(9968..10897)
                     /gene="ppk2"
                     /locus_tag="ACFJAS_001275"
     CDS             complement(9968..10897)
                     /gene="ppk2"
                     /locus_tag="ACFJAS_001275"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HFC3982522.1"
                     /translation="MTDRQLQPFENVELGEKQDQLQVFEKAVLEHEGKGSAEDSGTAP
                     LPENYPCRKRMLRAAYEAEKAKLQIELLKVQSWVKDSGQRIVSLFEGRDAAGKGGTIK
                     RFMEHLNPRGARVVALEKPTTTERGQWYFQRYIQNLPTAGEMVFFDRSWYNRAGVERV
                     TGFCEPNEYMLFMRQAPELERMLVASGIHLFKFWFSVSREEQLRRFISRRDDALKHWK
                     LSPVDIQSLDRWDDYTEAKNAMFFHTHTGDAPWVIIRSDDKKRARLNCIRYFLHQLDY
                     PGKDVKAIGKVDDKIVLVPDTRYKEKTIDVGHD"
     gene            11068..12942
                     /locus_tag="ACFJAS_001276"
     CDS             11068..12942
                     /locus_tag="ACFJAS_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC3982523.1"
                     /translation="MPYFALFDDAVSRRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            complement(13003..14619)
                     /locus_tag="ACFJAS_001277"
     CDS             complement(13003..14619)
                     /locus_tag="ACFJAS_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3982524.1"
                     /translation="MMRRVRRAWGLRRFCRRRPAVFFKEGKLKDSVYRVCRLCAALCV
                     WGAVGAYAAGLPDVRDDAAALRAQRAAAEGWAGMPPEGDSAANGGSRVIDGDFLLSRP
                     QLLEHVLRDALNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRPAEAVARY
                     RALHRDNAADERVLLDLAAAEFDDFRLKSAGLHFAEAAKLDLPAPVLENVGRFRKKTE
                     GLTGWHFSGGISPSVNKNANNAAPQYCLQGGGSRICSVTGPVRAAGLDYEAGAEKLTA
                     LADNHYLLFRANVGGTSYYFSRKSAYDDAFGRAYLGWQYKNARQTVGVLPFYQAQLSG
                     SDGFDAKTKPPADRRLAPYMLAHGAGVQLAHSYRLSRRSQLSVSLERYRQRYREQGRA
                     ERNDGWQDGLYVSLARRLGGSATVFGGWQFARFVPKRETVGGAVNNAAYRRNAVHIGW
                     AQEWGGTGGLNSRIAASYAHRNYKGIAAFSTEAQRNREWNVSLALSHDKLSYKGIVPT
                     LNYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            14862..15946
                     /gene="hupA"
                     /locus_tag="ACFJAS_001278"
                     /pseudo
     CDS             14862..15946
                     /gene="hupA"
                     /locus_tag="ACFJAS_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
     gene            15974..18403
                     /gene="hupB"
                     /locus_tag="ACFJAS_001279"
     CDS             15974..18403
                     /gene="hupB"
                     /locus_tag="ACFJAS_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC3982525.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLANDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVEGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
BASE COUNT         4492 a         5381 c         4980 g         3982 t
ORIGIN      
        1 tgagaagaaa tggacggatt gccaagtgta gcaaatattc gcacaaaggt cgtgcagaga
       61 ctgcttcaga cggcatcaga cacaaaaaga ccggcaacaa aaaagactgc acggggcagt
      121 ctttgcagat actatctttt tcataatatt ttttcctggc ccgacacagt ccatagcata
      181 tcaaaactgt tgttttcaat caggatatat atccccaatc ctaaataaac aacggcaaca
      241 aaccatctgc tatatttttc caaagtttct ccgacagaag ggacttgtgc caatttttgg
      301 gcagaaaaaa ccaagagata aatcatgact agaaaggtaa gtaaagccac tatcaaattc
      361 gctaaattta aggtagtaaa atatgggaca aagacaccaa tattgtcagc gccacaactt
      421 gcaaaagtaa tcatagcgac tagaaaaatc aggtttttat tatctttgcg caaaccctct
      481 ttggcaatag actctccatc ggaatctcct aacagcaaaa ctttgaggcc taggaaaatc
      541 ggaatcaagc cgagcaaacc taaaatctct ttactaggaa tataatctaa gacaaatgca
      601 aaaagcaaac ttagcaatat caggctaaca gagcctagaa attgtcctaa atagatgtta
      661 atgatgtctt ttctgctttt ttttggcaaa aaataacatt aggataataa gtaagtctac
      721 ggctgtccca gaatacagga ttattgaagt aacaacattt tgaatcataa aacatcccat
      781 tcaaatatat tttttaatgt attcaaacat taaactttgt agctgtcaac ttcaaccccg
      841 tcaaaatata gtggattaac aaaaaccggt acggcgttgc ctcgccttgc cgtactattt
      901 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atagataaga
      961 agtcagtgtg ccaaatatta aaaagccctg ccatcgaaat gatggcaggg cttaattctt
     1021 gcaaagcggc aatcagcgtt tgaacaggtt gccgaatttg ttgttgaatt tgtccacgcg
     1081 gccggtggta tcgacgattt tttgggtgcc ggtatagaac gggtggcaca gggagcaaac
     1141 ctcgatgtta aagttttctt tttccattgc ggatttggtt acgaatttgt tgccgcaaga
     1201 gcaggtaacg ttaacttcgc ggtagttcgg gtgaatacct tgtttcattt gatttccttt
     1261 caaaaaagcg ggcatagggg atgtacctat gcctcagaca agctcggcat tctcgctatt
     1321 ttctgttgtt tcgtcaagag gatattcgat aaaatgtaaa aagttctttt gaggaaggtt
     1381 tgatggaatc aaaattcttt ttcctgctgc tgcgttttgc cggttcggtg ttgccgccgt
     1441 cgcatatgcg cggcatcggc atcatcggca gacgggtgct cggttttctg gcgcggcgga
     1501 tttcgccgca tatcggacgc ggggcgtatg tgtttccgga tacggttttg ggcgacggct
     1561 cgggcatcgg ggcaaactgt gaaatctgcc gtggactggt ggtcggcaaa aatgtgatga
     1621 tggggccgga atgcctgctc tattcgacca accacaagtt tgaccgtgaa aacaaacgct
     1681 ttgagggcta cacggaaatc cgtccgatta cgttggagga cgatgtctgg ccggggcgca
     1741 gggtgattgt gatggcgggc gtaaccgtcg gacgcggttc ggtcgtgggc gcaggcgcgg
     1801 tggttacaaa agacattccg ccctactctt tggcggcagg caatccggca gtggtgaaaa
     1861 agaatctgcc ggaaggttga atgccgtctg aacgtgtcgg ggcggatgat ctgaaaaaac
     1921 aggaacatcg tttctgtttt ttgcgcttca gacggcatcg ctattgcgcc agcgcggtat
     1981 cgatttcttg gtagagtttg ccgaaatcgg gttctccgac gtaggttttg agaatttcgc
     2041 cttttttgcc gataaggacg gaagtcggat aaacctgtgt gccgaatgcc tgtccgacgg
     2101 ctttgtccgc atcataaatg acggtaaacg gcagtccgta gtctttgacg tattggcgga
     2161 cgctttctat cggatcgatg ggctgggcaa cggcgaggac ttggaaatct ttatttttgt
     2221 agtcgtttgc cgttttggtg actttgggca tttcgctcac acaacccgga caggagggaa
     2281 accaaaaatt aatcagggtg actttgcctt gcaggtcggc gttggaaacg gtttttccgt
     2341 gcaggtcggg cagggagaag gcgggcgcgg ttttactgtc ggggatgagg acggtggcaa
     2401 ggaggatgcc gatcagtgcg acggcggcgg cggtgagtat ttttttcatt cggacaaggc
     2461 ttccagtgcg cgggcaaggg tggcgggcag gctgacggtg cgttgtgtgg cggcgtggac
     2521 gggcatcagg gtgatgtcgg cttctgcggc ggttttgccg tttggcagtg taatcgtctg
     2581 ggtcagcaca atgcggcgca tgccgggggt tttcaggcgg catgaaaact gcaatacgtc
     2641 gccttcgacg gcgggacggc tgtatcggat gtcgatgcgg gcgacaatca gtatgaggcc
     2701 tgccaactcg tgcatcagtc cgcgtttttc aaaaaacgcc cagcgcgctt cctcgagaaa
     2761 ttcgaggtag cgtgcattgt tgacatgacc gtagccgtcg agatggtaat tgcggacggt
     2821 cagcttcatc agttcaggtt gatgggttgg aaggcttcgc gggcaagcgg ttcgtgttcg
     2881 aggtcggtga tgacggtaga aagctggatg tcgaaccatt cgttgaaaat gtcggcatcg
     2941 agtgcgggcc actcgcgttc gtcttcgcac cagtcggcaa gttcggcggc gaaaatgtct
     3001 tcgaaacggg cttcgatttc gtcccatact tcgtcggcgg tttcgcacgg gcggacaagg
     3061 taggaattgg catcggcttg gatgtcttcg agggtcagtc cgtcgaggtg gttgcccggc
     3121 agggtttgca gccagttcca aaaaggttct aaagggatga ggacgaatac gctgcggttg
     3181 acttcgtaca tggtttttcc tttgctgtcg cgcggtatgc gcaaaaaagg gattatagcc
     3241 caatctgtag tgtcggactg tccgttccga cggaagggaa tgccgtccga acacggattt
     3301 tcagacggca tggctttagg gttgtgttcc aggttgcgtt tcggcttccc ctgctgcttc
     3361 tgcctgtgtt tcggatacgg aatcttcgtg aacggcgggc tctgccgcgc cggtttcggc
     3421 actttcgacc aattcgtcga tgtcgatgtt atcttccgta ccttcggcag gtgttgcacc
     3481 ggtctgccgt gcgcggactt tcatatagag gtcgcgcgtg tagctgtatt tgtcgatggc
     3541 ggcttcgtcc agactgtcgg tcaaatcgag caggccttcg cgcgtactga cggcggcagc
     3601 ggcagtcgtg ccccagcgtc cggcaggggt atggaaaacg atattcttgg gcggataaac
     3661 agaggtaatg cccgtgccga gcgcgtcgcg gacggtggac gggcctaaga cgggcaacac
     3721 gaaataattg ctgtttttcc agccccacga ggcaaacgtg tcgcccaaag tgtttttatt
     3781 gtcgggaacg ccgcccgcgc cggcaatatc aatgagcccg cccaaaccga aggtggtatt
     3841 gatgccgacg cggacgaggt cttcgcttgc gcgtttgatg tccaaacgca agatattgct
     3901 gccgaaactg accacgtcgc gcaggttgtt aaaaaaattg gacacgccgg cgcggacggg
     3961 tttcggcgta actttgcggt agccgcgcgc ggcaggggcg aaaatgtagc ggtcggcttg
     4021 gtcgttgaat ttggaaacgg cgcggttgta gccttcataa gggtcggcgg ggcgggtttc
     4081 tgcaaatgca ggggcggaag cgaacccgat cagcaggagg atggcgtagg cggttttttt
     4141 catgatttca gccagtcttt gatttcgtac agttcggaca gcgcgcgcac ggattcggga
     4201 atgccggtca gcctgacgct gcctttgcag ccgcgcagca cttcgagcag cagcgacacg
     4261 caggcggaat cggcacgttt gacacctccg aaatcgacgg caatggtttc tttcagacgg
     4321 cattgttgcc ggaaacgccc aaatgcgtcc ccggtcaggg ttttgacggt gatgtcgccg
     4381 ccgatgtgca gtgttccgtt tttgagttct gtatgcatag cgtttgttcg aaaaacccat
     4441 accgccctcg gacggtatgg tttgtcggtt atttgccgcc gtttttggct ttcaactcgg
     4501 caatcagccc gtcgatgcct ttggctttga tgatttcgcc gaattggttg cggtacacgg
     4561 taaccaggct cgcgccttcg atggcgacgt tgtaggtacg gtatttgccg ccgctttggt
     4621 aggtggtaaa gtccatattg acgggcttct gaccggggat gccgacttcg gcacggacga
     4681 cgatttcctt gccgccctta ttgacgatgg gattgtcttt gacgttgacg gtcgcgtttt
     4741 tgaatttcag catcgtgccg gaataggtgc ggatcagcag ggtttgaaat tctttggcca
     4801 acgcttgttt ttgcgcgtcg gacgcggtac gccaagggtt gccgaccgcc aatgcggtca
     4861 tacgttggaa atcgaaatag ggaaccgcat aggcttcggc ttttgggcgt gcagaagccg
     4921 cgtcgccgct tttgaggatg gtcaaaacct gtgtggcgtt ttggcggatt tgtcccactg
     4981 cgtcggccgg ggaggcaaat gccatgccga tgctcaaaat accgatgccc aatgcgctga
     5041 tgaaggagga ttttttcatg atgtctttcc tgaaaatgga tgtgtatgtt tattctgcgg
     5101 ctttttccgc attgccgccc tcagcgtttt tctcggcgaa gctggtcatg aatttaccga
     5161 tcaggttttc cagaaccatt gcagaactgg ttacggagat ggtgtcgccg gcagcaaggt
     5221 tttccgtatc gccgccctgc tgcagcccga tgtactgttc gcccaaaagt cccgaagtca
     5281 ggatttgcgc ggaaacgtcg ctgctgaact gatacttgcc gtccaaatca aggcgcaccc
     5341 tcgcctgata ggatttcggg tcaagcccga tagcgccgac gcgcccgacc aatacgcctg
     5401 cggatttgac gggggcattg accttcaaac cgccgatgtc gccgaaatcg gcataaacgg
     5461 cgtaagtttt gtccgaaccg ccgaacgccg cgccgcccgc cacgcggaaa gcgagaaagg
     5521 caaccgccgc cgcgccgatc aggacgaaca gtccgaccca aaattccaat atgttctttt
     5581 tcattaaagt tccttgaata tccgatgttc cgcgtttcgt cttcagacag tctgtcaatc
     5641 tgtaaacatc cacgcggtca atataaaatc gaccgccaaa atcgtcaggg cggacgaaac
     5701 caccgtgcgc gtgctggcgc gcaaaatgcc ttccgaagtc gggatgcagt ggaagccctg
     5761 atgcacggca atcagcgtta ccgccacgcc gaacgcggcg gatttgatca aaccgttgat
     5821 tacatcgtaa tgtatcgtaa tgttgttctg catctgcggc cagaaaatac cgctgtccaa
     5881 acccagccag ctcacgccga ccaaatacgc gccgaaaatg cccgcgacgt tgaaaatcga
     5941 agccaaaagc ggcatagaaa acacgcccgc ccaaaaacgc ggggcaacca cgcgggcgac
     6001 ggggttgacc gccatcacgt tcatcgcttc gagctgtccg gtcgttttca tcaaaccgat
     6061 ttcgctggtc atcgcaccgc ccgcgctgct ggcaaacaga atcgccgcca acacgggacc
     6121 cagttcgcgc aacagagaag ccgcgaccat atagcccaaa atatcggcgg atttgaattt
     6181 cgacaactgc gtatagccct gcaaacccaa aaccataccg acgaacagcc ccgaaacggc
     6241 aacaatcagc accgacagca cgccggcaaa atacacttgg cgcacgctca ggcgcggacg
     6301 ggcgaaagcc gtgccggatt tcgccaaaat gttcagcaga aacagcgtga tactgccgaa
     6361 ggattgaata aggccgaggg ttttcgcccc gacggaacgg ataaaattca taaatttcta
     6421 tatgtaaggt tcaacggttt cagacggcac ctgctcattt acccgagcaa atcctgctgc
     6481 aacgacgttt gcgccggata gcggtatgcc acggggccgt ctgccagccc gccgacaaac
     6541 tggcgcaccc aaggcgaatc cagttcgcgc atttcctgcg gcgagccgga gaacataatt
     6601 tcgccgtgcg ccaagaaaat tacctgatcg acgatttcca aagatttttc aatgtcgtgc
     6661 gttaccataa tgctggtcga acgcaaggcc ttgttgacgc ggctgatcaa gtgggcaatc
     6721 acgcccaagg aaatcgggtc gaggccggta aacggttcgt cgtacaacat aatttcgggg
     6781 tcgagcgcga ttgtgcgtgc cagcgcgacg cggcgcgaca tcccgccgga caactcggac
     6841 ggcatcaggt tttccacgcc gcgcagaccg accgcgttca atttcaacaa aaccaaatcg
     6901 cgaataaccg cttccggaag ttgcgtcagt tcgcgcatcg gaaaagcgat attgtcgaat
     6961 accgacaaat cggtaaacag cgcgccgtgt tggaacaata cgcccatacg gcggcggtgt
     7021 tcgtagagtt cgtcagccga aaaacccgcc aaatcccgtc cttcaatcaa aacctgcccg
     7081 gactgcggac gaatctgtcc tgtaatcagc ctcatcagcg tggttttgcc gctgcccgaa
     7141 ccgcccatca cggcggcaaa attgccttgc ggaatgctga aattgatgtc gttcagaatc
     7201 gggcggtcgc catacgcgaa ggcgacgtct ttcatttcga taaaggggga tgggctcatg
     7261 tacgggcgga cggtaggttt gacggcgtgt attttaaggc ttatcgggaa gacgggcaat
     7321 tttcagacgg catacggacg gcaagtgttg tgaaaatgcc tttgtcggcg gcggattgtt
     7381 tgctgtggcg aaaaatgtta tctttcaaat gataaccttt atcagaaaac tatggaaaaa
     7441 gcagaacatt tgaacagcag ccggttcgtc aatctggtca aaagcggcgg cggcttatgt
     7501 ggagggcagc taccgtttcg atactttgtc caacggcatt tccatccacg gcggtacggt
     7561 aacggcacgg tgcgattttt gcagcagccg cctcgccgaa ccttatgtgt cgttcgtgct
     7621 gttgctggaa gggcgtttgg acttcggcat caaccgccgc cgcttccgca tcgatgcgga
     7681 cggcggcaag attgtcctaa ttgccgtcgg ggaagaagtc ctgttcagcc gctatcttta
     7741 tcgcggcggc aaaacggtca aaatgaccat taaaggtatg gaacaatggc tgccgcgtcc
     7801 ggaatacgcg cgtttcgcac ccctgcttta ccgcgaaccg gtcaggatat gggatttgcc
     7861 cccaaacctg cgcggcttgg cggcatcctg cctgcaaacc gtcccaaagg ggcatttggg
     7921 cgaaacattg cgccgcgagg cggacgtgtt gcggctgctg tcggatttgt gggacacggt
     7981 ttcagacggc atcgagccgg cggcggggca aacggcggaa gcagacgcta tgccgtctga
     8041 agacttcagc cgcaccctga atgccgcgtt cgacggcggc gcacaccaag tcaaccggct
     8101 gacagccgcg ctgaacatca gcgaaaggac gctgcaacgc cgtatgcgcg accatttcgg
     8161 cattacggca agcgagtggc tgcaccacaa acaaatgcag cacgcgctct atctgttgca
     8221 aaacgggggc aaaaacgtgg gcgaaaccgc gtatttgtgc ggctagcgcc acgtttccag
     8281 ctttattcag gcattcaggc aatatttcgg cagcacgcct gcggaaacta aaaaagaaaa
     8341 ccggtaagcc gtatttgact tgaaacccaa aatccgcgtg taaagaaact tgtacggtaa
     8401 tttatttggg aataaaccaa aactacatcc gactacaaaa ctggagggcc cgaaatgaaa
     8461 caattggcca tgtacatcaa cggacgcttt gaaaacgatt tcaacggcga atggcgcgac
     8521 gtattgaacc cgtccaccga agaaaccatc gcccgcgaac ccaaaggcgg cagggcggac
     8581 gttgaccgcg gcgcgcgcgg cgcaaccggc ttgggagcgt ctgcctgcgg tcgaacgcgg
     8641 cgcgtatttg cgtaaaatcg cccaaggcat acgcggacgc gccgacgagc tgaccgacac
     8701 catcgttgcc gaaggcggca aaaccaaaga cttggcgcgt gtggaagtga tgttcactgc
     8761 cgactatctc gattaccaag ccgaatgggc gcgccgttac gaaggcgaaa tcatccaaag
     8821 cgaccgcccg cgcgaaaaca ttttatcgtt caaacgtccg ctgggcgtga ttgccggcat
     8881 tttgccgcgg aacttcccct tcttcctgat cgcccgcaaa atgggcccgg atctggttac
     8941 gggcaacacc atcgtcgtca aacccagcag cgtaaccccg atcaactgcc acatcttcgc
     9001 cgaaatcatc gatgcggtcg gactgcccgc aggcgtgttc aacgtggtgg acggtccggg
     9061 tgcggaaatc ggcaacgcct tgtccgccca tccgcaagtc gatatggtca gcctgaccgg
     9121 ctcggttgaa gcgggtcgcc aagtgatgga agccgcttcc gccaacatca ccaaagtctc
     9181 gctggaactc ggcggcaaag cccccgccat cgtgttgaaa gacgcggatt tggatttagc
     9241 ggtgaaatcc atcttggctt cgcgcgtcgg caacaccggt caaatctgca actgcgccga
     9301 acgcgtctat gtccacagca gcctgaaaga cgcgttcatt gaaaaaatga ccgccgcgat
     9361 gaaaggcgtg cgctacggca accctgccga agccgaagca ggcgcgccgg aaatgggcac
     9421 gctgattgaa gagcgcgccg tcaaagccgt tgccgaaaaa gtggaacggg ccgtcaaaca
     9481 aggtgcgaaa ttggtttgcg gcggcaaacg caccgaagga cgcggctatt tcttcgagcc
     9541 taccctgctg accgacaccg acaacagtat ggacattatg aaagaagaaa ctttcggcct
     9601 cgtgctgccc gtttccgctt tcgacacgct cgaccaggtc atcgccctgg caaacgactg
     9661 cgaattcggt ctgaccagct ccgtgtacac caccaacctg aacgaagcct tctacgttac
     9721 ccgccgcctg caattcggcg aaacctacat caaccgcgaa aacttcgaag ccatgcaggg
     9781 cttccacgcg ggctgggaaa aatccggcat cggcggcgcg gacggcaaac acggtttgga
     9841 agaatacctg caaacccaag tcatttatct ggaaaccgac atctgatgcc ggtttgacac
     9901 acggatagaa aatgccgtct gaacctgaaa tcgggttcag acggcatttt tattacttca
     9961 ccggcaatca gtcatgaccg acatcgatgg ttttctcttt gtaacgcgta tcgggaacaa
    10021 gaacgatttt atcgtccact ttgccgatgg ctttcacgtc tttacccggg tagtccaact
    10081 ggtgcaggaa gtagcggata cagttcaaac gggcgcgttt tttgtcgtcc gaacggatga
    10141 tgacccaagg cgcgtcgccg gtgtgggtgt ggaagaacat cgcgtttttg gcttcggtgt
    10201 agtcgtccca gcggtcgagc gactggatgt ccacggggga aagtttccag tgtttcaggg
    10261 cgtcgtcgcg gcgggagatg aagcggcgca gttgttcttc gcgggatacg gagaaccaga
    10321 atttgaacag gtggatgccg ctggcgacaa gcatacgttc caactcaggg gcttggcgca
    10381 taaagagcat atattcgttg ggttcgcaga agcccgtcac gcgctccacg ccggcgcggt
    10441 tgtaccatga gcggtcgaag aacaccattt cgcccgcagt cggcaggttt tggatgtagc
    10501 gttggaaata ccattgtccg cgttcggtag tggttggttt ctccaaagca accacgcgcg
    10561 cgccgcgcgg gtttaaatgt tccataaagc gtttgatggt gccgccctta cctgccgcat
    10621 cccggccttc aaacaggctg acgatgcgct ggccggagtc tttgacccag ctttgcactt
    10681 tcagcagctc gatttgcagt ttggcttttt cggcttcgta ggcggcgcgg agcatacgtt
    10741 tgcggcaggg gtagttttcg ggcagcgggg ctgtgccgga atcttcggca gaacctttgc
    10801 cttcatgttc caaaacagct ttttcaaata cttggagctg gtcttgcttt tcgcccaatt
    10861 ctacgttttc aaacggttgc aactggcggt ctgtcataaa ggtctcctat tgtatttcag
    10921 gttggaaatg aaagtccgtt ttcacaattt tacactttcg ccaccgcttt ctctacataa
    10981 aattacattt tgttgcccga atttgccccg ccgtctgaaa atccgtgtaa aaggggcgta
    11041 taattaaaac atttgccccg aattgccatg ccttatttcg ccctgtttga cgatgccgtc
    11101 agccgacgcg caaaactcta tcaaaatcat gtggaaagcc gttttttccg tcccgaagaa
    11161 ctcgatgctt tagacggcgc gctgcaatcg ggatggcaaa aagggctgca ttcggtgttg
    11221 tttgcagact acgaattcgg tttgccgctg atggggatgg aatccgaacg cggcggcaac
    11281 attgccctgc actggtttgc cgactgcgcc gacaccgatg ccgaaagctg gcttgcccga
    11341 cattcagacg gcatccccgc cggtatttcc acgccgcaat cctccgtatc cgaaaccgat
    11401 tacctcgacc gcatccgcca aatccacgaa gccatccggc gcggcgacac ctatcaaatc
    11461 aactacacca cccgcctgca cctgcaagcc tacggcaacc ccgtcagcct ctaccgccgc
    11521 ctgcgccagc ccgtccccta tgccgtattg tcccacctgc ccgatgaagc cgggcaatcc
    11581 gcgtggacgc tgtgtttctc gcccgaactc ttcctcaaca tcgcttcaga cggcaccatc
    11641 agcaccgaac cgatgaaagg caccgcgccg attttgggcg acggacaaga cgaacgccgc
    11701 gccgccgagt tacaaaccga cccgaaaaac cgcgccgaaa acgtgatgat tgtcgatttg
    11761 ctgcgcaacg acctcggcaa aatcgcccaa accggtaaag tatgcgtacc cgagccgttt
    11821 aaagtatcgc gtttcggcag cgtctggcag atgaccagca ccatccgggc gcaagccctg
    11881 ccggacacct cgttcgccga caccctccgc gccgccttcc cctgcggcag catcaccggc
    11941 gcgcccaaaa aaatgagtat gcagattatc gaatcgctcg aaaccgaagc gcgcgggctt
    12001 tatacgggca gcatcggcta tttgaacccg tgttccggcg gcttggggtt tgaaggcgcg
    12061 ttcaacgtcg tcatccgcac cctgtcgctc aaacccgttt cagacggcat tgtttcaggc
    12121 atcggcggca cggattcaaa cgcgcaagcc cgaacggcag gacaaggcgg ggaaacgccg
    12181 catccgtttg aagccaatcc gccgtatcgg ggcgtgtacg gtgtcggttc cggcatcgtc
    12241 atcgacagcg accccgccgc cgaatatcgc gaatgcggct ggaaagcccg attcctcaac
    12301 gaactgcgcc ccgatttcgg catttttgaa accctgcgcg cggaaaacag gcaatgcgcc
    12361 ctgctcgacc gccacctatg ccgtctgaaa acctccgccc aagccctcaa cctgcccctg
    12421 cccgacggct gcgaaaatca aatcaaacaa tacattgccg acttgcccga cggcgcgttc
    12481 cgcatcaaag ccctgctcgc ttcagacggc atcagcctgt cccacgccgt tttaaaccgt
    12541 ctgaccgaca aacagcgcgt catcatttcg ccgaccatcc tgcccgcaca aaactacctg
    12601 cgccgcttca aaaccacaca ccgcgccatc ttcgaccaag cgtggcaaac cgccgaaacg
    12661 caaggcgcgt tcgacagcct gtttttcaat tcggacggca tcctactcga aggcggcagg
    12721 agcaacgtgt tcgtcaaaca tcgcggacaa tggctcacgc cctctttaga tttagacatt
    12781 ttaaacggca taatgcgcca agccgtgtcg gacgaaccgc aaaaatattt gcacacaaat
    12841 caagtgattg aaacacacat cacacaaaaa acactgcaag gagccgaaga aatccgcctc
    12901 tccaacgcct tgcgcggcgt atttgccgcc gtccttgcct gaacgcgcaa aaatgccgtc
    12961 cgaacctgct gccgcaaagt tcggacggca ttatcccacc attcaaaacc gccaatccgc
    13021 cgacacaaac acctcgctgt tgcggcgttt tgcatacggc acattgcttt ccgtcttacc
    13081 gaagcggtag ttcaacgtcg gcacgatgcc tttgtacgac aacttgtcgt ggctcaaagc
    13141 cagcgagaca ttccattcgc ggttacgctg cgcctccgtc gaaaatgccg caatgccttt
    13201 gtagttgcgg tgggcgtaag acgcggcaat ccggctgttc aaaccgcccg tcccgcccca
    13261 ctcctgcgcc caaccgatat ggacggcgtt gcggcggtag gcggcattgt tgaccgcgcc
    13321 gcccaccgtt tcgcgtttcg gcacaaaccg cgcaaactgc cagccgccga acacggttgc
    13381 cgagccgccc aaccgccttg ccagcgacac ataaagcccg tcctgccaac cgtcgttgcg
    13441 ttccgcccgg ccttgttcgc ggtagcgttg gcggtaacgt tccagcgaca ccgaaagctg
    13501 cgaccggcgg ctcaggcggt aagaatgcgc caactgcaca cccgccccgt gcgccagcat
    13561 atacggcgca agccggcggt cggcaggcgg tttcgttttc gcatcaaagc cgtcgctacc
    13621 cgacaattgc gcctgataaa acggcaaaac ccccaccgtc tgccgcgcat ttttatactg
    13681 ccagcccaaa taagccctgc cgaaagcatc gtcataagcc gatttgcggc tgaaataata
    13741 gcttgtgccc ccgacattgg cgcggaacag cagataatgg ttgtccgcca gcgcagtcag
    13801 cttttccgcc ccggcctcat aatccaaccc tgccgcacgg acaggtccgg taacgctgca
    13861 tatccggctg ccgccgccct gcaggcagta ttgcggcgcg gcattgttgg cgtttttgtt
    13921 cacagaaggg ctgatgccgc ccgaaaaatg ccagcccgtc agcccctccg tttttttccg
    13981 gaaacgcccc acattttcca aaaccggcgc cggcaaatcc aattttgccg cctccgcaaa
    14041 atgcagccct gccgacttca gccggaaatc gtcaaactcc gccgccgcca aatccagcaa
    14101 aacccgctcg tctgccgcat tatccctgtg caacgcccga tagcgcgcca ccgcctccgc
    14161 cggccttccc gccaatttcg ccagcaaagc ccgcgccctg ccgtacaaca ccgcatcata
    14221 atccggcagc ttggcataca aatccgccaa cgaagcgatc aaatccgcct gattgccgtt
    14281 gagcgcgtcg cgcaaaacgt gttccaacaa ctgcgggcgc gacagcagga aatccccgtc
    14341 gattacgcgc gaaccgccgt ttgcggcaga atccccttcc ggcggcattc ccgcccaacc
    14401 ctccgcagcc gcacgctggg cgcgcaacgc cgccgcatcg tcccgaacgt cgggcaagcc
    14461 ggcggcatac gcgccgaccg caccccaaac acacagcgcg gcacacagcc ggcatacccg
    14521 atacacacta tccttcaatt tgccttcctt aaaaaacacc gccggacggc ggcggcagaa
    14581 acgccgcaag ccccacgcgc ggcggacgcg gcgcatcata tagtggatta acaaaaatca
    14641 ggacaaggcg acgaagccgc agacagtaca gatagtacgg caaggcgagg caacgccgta
    14701 ccggtttttg ttaatccgct ataaaaaaca aaggtagaaa cgcggcaggc aaacgcaggc
    14761 acgtccgatt tccgcgccgc cccataaaac agttgacatc atataacatt taaattgata
    14821 atcaaaacta ttattaatac atcatcaaga ttgagtatgc gatgaaatac aaagccctgc
    14881 ccttactgcc gcttgccgcc gcccttgccg cctgtgccgg gtgggggggg tagccgaacc
    14941 gcacgtcccc gtgtccatcc ccaccgccac gccgctgccc gccggcgagg taacgttatc
    15001 aagcgataac ggcaatatcg aaaacatcaa caccgccggc gccggaagcg catccgacgc
    15061 gccgagccgc agcagacgct cgctcgatgc cgccccgcaa aacacatccg gcatttccat
    15121 tcggcagcgg gaagtagaaa aagactattt cggctataaa tcaaaagaaa catcattcat
    15181 cttcaaaaca cccggcggcg cgcaatacgc cctttcatcc tacgccgacc ccataaccgt
    15241 cagctactcc tcccccgatt tcaaaattcc cgaccgccac gcagggcagc ggctcgccga
    15301 cggcagccgc atctttatct gctgcagcga ctccggggct acttcgtatg cggaaattac
    15361 caaacaagat tatatgaaat tcggggcgtg gataggtccc aacggcgaaa tcgacctctt
    15421 cgccggcggc ttccccgtcg gcaaaacgcc gcctcctgcg ttctcctacg gcagcagcac
    15481 gcccgaaacc gcattgtcaa aaggtaaaat cacttatcag gtttggggca tccgcgtcag
    15541 aaacgggcaa tttgttactt cctcttatac gccgccgaaa agcggttctt attatgggac
    15601 acttgccaat acccccgtcc tttcctttat caccgccaat ttcaacagca acacactggc
    15661 aggcaaaatc ctcggcaaca gcgattacgg gccggatgtg gatattcaaa acgcaacgat
    15721 taccggtccg accttttccg gcgatgccac gtccggcggg aaaaacggca aattggaagg
    15781 caagtttttt ggcaagttcg ccagcacccg cagcagcgaa gtcagcatag gcggcaaaat
    15841 cacctttgac ggcgaccgct ccctcgatac cgtattcggc ggtgtgagtt atgaaaaaaa
    15901 acttgatgac accagtcaag ataccaacca ccttactaaa caataaacca cctcatccct
    15961 gcaaaggttt cttatgccca ttcccttcaa acccgtattg gctgccgccg ccatcgccca
    16021 agcgtttccc gcctttgcgg cagaccccgc gccgcagtcc gcccaaacgc tgaacgaaat
    16081 caccgttacc ggcacgcaca aaacccaaaa actcggcgaa gaaaaaatcc gccgcaaaac
    16141 cttagacaag ctcttggcca acgacgaaca cgacctggtg cgctacgacc ccggcatttc
    16201 cgtcgtcgaa ggcggcaggg cgggttctaa cggctttacc atacgcggcg tggacaaaga
    16261 ccgcgtcgcc atcaacgttg acgggctggc gcaggcggaa agccgctctt ccgaagcctt
    16321 ccaagaattg ttcggcgcgt acggcaactt caacgccaac cgcaacactt ccgagccgga
    16381 aaacttttcc gaagtaacca tcaccaaagg cgcggactcg ctcaaatccg gcagcggcgc
    16441 attgggcggc gcagtcaatt accaaaccaa atccgcaagc gactatgttt ccgaagacaa
    16501 gccctaccat ttggggataa agggcggcag cgtcggcaaa aacagccaaa aattcagcag
    16561 cattaccgcc gccggcaggc tctttggttt ggatgcctta ttggtttata cccgccgctt
    16621 cggcaaagaa accaaaaacc gctcgaccga gggcgatgtc gaaattaaaa acgacggata
    16681 tgtctttgac ccggccaacc cctcgcccag cagatacctt acctacaaag ccacaggggt
    16741 tgctcgctcc caacccgacc cgcaagaatg ggtaaacaaa agcaccctgt tcaagctggg
    16801 ctacaatttc aacgaccgca accgcatcgg ctggattttt gaagactcgc gcaccgaccg
    16861 ttttaccaac gagctgtcta atttgtggac gggtacgacc acgtctgccg caacgggcga
    16921 ctaccgccac cgccaagacg tgagctaccg ccgccgcacc ggcgtcgaat acaaaaacga
    16981 attggaacac ggcccgtggg acagcctcaa gctgcgctac gacaagcagc gcatcgatat
    17041 gaacacttgg acttgggaca tcccgaaaaa ttacgataca aacggcatca acggcgaggt
    17101 ttaccattcg ttccggcata tccgccaaaa caccgcgcaa tggactgccg attttgaaaa
    17161 acaactcgac ttttccaaag ccgtttgggc ggcgcaatac ggcttaggcg gcggcagagg
    17221 ggacaatgcc aactcggatt acagttattt cgcaaaactg tacgacccca aaatcctcgc
    17281 ttccaaccaa gccaaaatca cgatgctgat cgaaaaccgg tcgaaatata aatttgccta
    17341 ttggaacaat gtgtttcact tgggcggcaa cgaccgcttc cgcctgaatg cgggcatacg
    17401 ctacgacaaa aacagcagca gcgcgaaaga cgatccgaaa tacaccaccg ccatccgggg
    17461 gcagattccc catttgggtt cggaacgcgc gcacgcgggc ttcagctacg gcacagggtt
    17521 cgactggcgg tttaccaagc atctgcactt gttggcaaaa tacagcaccg gcttccgcgc
    17581 accgacttcg gacgaaacct ggctgctgtt cccacacccc gatttctacc tgaaaaccaa
    17641 tcccgaactg aaagcggaaa aagccaaaaa ctgggaattg ggcctggcgg gcagcggcaa
    17701 agctggcagc ttcaagcttt cgggcttcaa aaccaaatac cgcgacttta tcgaattgac
    17761 gtatatgggc gtttcgtcag acgataaaaa caaccccaga tacgccccgc tttcagacgg
    17821 cacggcattg gtcagctcgc ccgtttggca aaaccaaaac cgcaccgccg cctgggtgga
    17881 aggcatagag tttaacggca cttggaacct cgacagcatc gggctgccca aagggctgca
    17941 caccggcctc aacgtcagct acatcaaggg caaggcaacg caaaacaacg gcaaagaaac
    18001 gcccatcaac gcgctttcgc cgtggacggc ggtttacagc ctgggctatg acgcgccttc
    18061 caaacgctgg ggcgtcaacg cctacgccgc gcgcaccgcc gccaaaaagc cgtccgacac
    18121 cgtccacagc aacgacgact tgaacaaacc gtggccttat gccaaacaca gcaaggccta
    18181 tacgctgttc gacctttccg cctacctcaa catcggcaaa caggttacgc tccgcgccgc
    18241 cgcgtacaac attaccaaca agcagtacta cacttgggaa tctttacgca gcgtccgcga
    18301 gttcggcacg gtcaaccgcg tcaacaacaa aacccacgcc ggcatccaac gctttacctc
    18361 gccgggcagg agctacaatt tcaccatcga agcgaagttc taagctcctg atgtgccaat
    18421 gccgtctgaa acccgatgat ttgggtttca gacggcattt tttacggtcg caccgccgcc
    18481 ccctttcagg cttgtaccaa gcccaatccc gcaactctcc gtcattcccg caactctccg
    18541 tcattcccag ggaagtggga atctagaaac gcaaagccac aggaacttat ccgaaacaac
    18601 agcaacctaa aattcgtcat tcccacgaaa gtgggaatcc agttcgttga gtttcagtta
    18661 tttaaaataa attttgaaac tttaatccca tcattcccgc gaaagcggga atccggaacg
    18721 taaaatctaa agaaaccgtt ttatccgata agtttccgta ccgacggggc tggattcccg
    18781 cctgcgcggg aatgacggcg gagcggtttc tgttttttcc gataaattcc taaaa
//
LOCUS       DAXFZR010000044        18672 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000044 DAXFZR010000000
VERSION     DAXFZR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18672
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.044"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..207
                     /locus_tag="ACFJAS_001280"
     CDS             <1..207
                     /locus_tag="ACFJAS_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3982526.1"
                     /translation="INRSKEFADRRNHINGIGNFWNQAKRALRKYNGIDRKPFPPFLR
                     ECEFRLNFGTPPRQLKILRDRCGI"
     gene            complement(281..1702)
                     /locus_tag="ACFJAS_001281"
     CDS             complement(281..1702)
                     /locus_tag="ACFJAS_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC3982527.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(2061..2420)
                     /locus_tag="ACFJAS_001282"
     CDS             complement(2061..2420)
                     /locus_tag="ACFJAS_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC3982528.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2417..3022)
                     /locus_tag="ACFJAS_001283"
     CDS             complement(2417..3022)
                     /locus_tag="ACFJAS_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HFC3982529.1"
                     /translation="MTIMNIDTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPYFQFEGTDSDGIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(3069..3551)
                     /locus_tag="ACFJAS_001284"
     CDS             complement(3069..3551)
                     /locus_tag="ACFJAS_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC3982530.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3755..3852
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4063..5163
                     /gene="gcvT"
                     /locus_tag="ACFJAS_001285"
     CDS             4063..5163
                     /gene="gcvT"
                     /locus_tag="ACFJAS_001285"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC3982531.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVVGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPDTEAVAFFKALQTA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGRTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRSGQKQFD"
     gene            5310..5849
                     /locus_tag="ACFJAS_001286"
     CDS             5310..5849
                     /locus_tag="ACFJAS_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982532.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5901..6287
                     /gene="gcvH"
                     /locus_tag="ACFJAS_001287"
     CDS             5901..6287
                     /gene="gcvH"
                     /locus_tag="ACFJAS_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC3982533.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6440..7687
                     /gene="hemA"
                     /locus_tag="ACFJAS_001288"
     CDS             6440..7687
                     /gene="hemA"
                     /locus_tag="ACFJAS_001288"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC3982534.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7934..10240
                     /locus_tag="ACFJAS_001289"
                     /pseudo
     CDS             7934..10240
                     /locus_tag="ACFJAS_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753899.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            10369..12336
                     /gene="nosZ"
                     /locus_tag="ACFJAS_001290"
                     /pseudo
     CDS             10369..12336
                     /gene="nosZ"
                     /locus_tag="ACFJAS_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12621..12776
                     /locus_tag="ACFJAS_001291"
                     /pseudo
     CDS             12621..12776
                     /locus_tag="ACFJAS_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            12773..13864
                     /locus_tag="ACFJAS_001292"
                     /pseudo
     CDS             12773..13864
                     /locus_tag="ACFJAS_001292"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13922..14818
                     /locus_tag="ACFJAS_001293"
     CDS             13922..14818
                     /locus_tag="ACFJAS_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3982535.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14815..15645
                     /locus_tag="ACFJAS_001294"
     CDS             14815..15645
                     /locus_tag="ACFJAS_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3982536.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15642..16136
                     /locus_tag="ACFJAS_001295"
     CDS             15642..16136
                     /locus_tag="ACFJAS_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC3982537.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16270..17931)
                     /locus_tag="ACFJAS_001296"
     CDS             complement(16270..17931)
                     /locus_tag="ACFJAS_001296"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC3982538.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(18061..18669)
                     /locus_tag="ACFJAS_001297"
     CDS             complement(18061..18669)
                     /locus_tag="ACFJAS_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC3982539.1"
                     /translation="MSYRLNAAPMFNDNPVVYGKIKLQSWKARRDFNIVKQDLDFSCG
                     AASVATLLNNFYGQKLTEEEVLEKLGKEQMRASFEDMRRIMPDLGFEAKGYALSFEQL
                     AQLKIPVIVYLKYRKDDHFSVLRGIGGNTVLLADSSPGHVSMSRAQFLEAWQTREGNL
                     AGKILAVVPKGRDAGGDKAFFTRSPKRQTEFAVGQVKWWRAY"
BASE COUNT         4543 a         4845 c         5014 g         4270 t
ORIGIN      
        1 atcaaccgtt ccaaggaatt tgcagaccgt cggaaccaca ttaacggcat tgggaacttt
       61 tggaatcagg caaaacgcgc cttgcgaaaa tacaacggaa tcgatcgtaa acctttcccg
      121 ccgttcttga gggaatgcga atttcgactt aacttcggca caccgccccg gcagctaaaa
      181 atcctgcggg atcggtgtgg aatttaggga taatctagta cagccccttt gattatgccg
      241 gaatttaaaa tgccgtctga atgttcagac ggcatagcgt ttacagtagt ttgaaaacga
      301 aaaagacgag ggtatgtacg atgaagacgg gtgtcaggaa ggcgaccgac cacatcatat
      361 agccgaagaa agtcggcatc ggtacgccgc gctgttcggc aatggccttg accatgaagt
      421 tcggtgcgtt gccgatgtag gtcagtgcgc ccatgaatac cgaacccata gaaaccgcca
      481 gcagcgaatg aaacagggga cccgtcatta aggcttgggc atcgccgccc gccatattga
      541 aaaacacgag ataagtgggc gcgttatcca agaatgccga caatatgccg ctcatccaga
      601 aatacatcgt attaatcgga tgacctgccg tatcgtgaac cagcgatacc accccgccca
      661 gcgcgcctgc ctcgcctgct ttcagaatgc tcaggacggg gaagatggtg atgaagatgc
      721 cgaggaagag tttgcccact tcggcgatgg gttcaaagtt gaattcgttg cctgcgcgga
      781 cttgtttggg cgtgattgcc atagatacgg cggtcaatgt aatcaggatg acatcgcgga
      841 caaggttttg cagggcgtaa cggctgccga ggatttcaaa tcccgggtgt tcgggtttcc
      901 aaaggccgga cattagaacc gcgccgacca cgcccgaaag caggaggaag ttccatttgc
      961 cgaagatggc gattttttcg ggtttttcct gttgtgccgg cgtatcttgt gcaatgcttt
     1021 cctgtttgaa gaaacggttg tcgatgaaat agaaggcggt caacaggaca gcggtgctaa
     1081 tcaggacggg ggcgaacata tgtttgaccg tccacatgaa atctacgcct ttgaggaagc
     1141 cgaggaagag tggggggtcg cccaaagggg tcaggccgcc gccgatgttt gcaaccagga
     1201 aaatgaagaa gatgacgatg tgcacgcggc gggtacggtc ttggttggct ttcagcagcg
     1261 gacgaatcat cagcattgcc gcgccggtcg ttcccatgat agaggcaagt gccgtaccga
     1321 cggcaagcag ggcggtgttg agcttgggtg tgccgttcaa gtcgccccaa accaaaatgc
     1381 cgcctgaaat ggtgtacagg gcaagcagca gcaggatgaa ggggatatat tcttcaacga
     1441 gtgcgtgtgc gacggtatgg ataccggcgg acgcgccaaa aaccaaactg aacgggatga
     1501 ggaagagcaa tgtccaaaag gcggtgattt tgccgtaatg gtgatgccag gtatgcgaaa
     1561 aaaacaaggg acccaatgcg atagacagca aaatcagggc aaagggcaag ccccacagca
     1621 ggtttaggtt tgcgccgtcc gaatctgcgg cgtaaaccga tgccgggaaa agcattagtg
     1681 aaaacagggg taggtggcgc atcgtgtttc ctcgattcaa gcactgcctt gcgcggcgcg
     1741 cgggagtgat acaggcttcg tgccgcccgg acataggcgg actgtgctgc ctgtttgttt
     1801 ttgaaaaaag ttttccgatt gattgtaaag taaataatca ggataaacca gtttcccaaa
     1861 ccggaaggcg gcgggaaggc ggattgccgt gcttgggaat atatgttctt tttgataaat
     1921 aattttattt aaaacaaata aatatatgtc tttaataaat aatctgtcga aaacgaaaaa
     1981 taaatttatt ttaacatata tttgcaatga aacaggtttg ccccacccgt ttgtttgccc
     2041 ttatcccttt cagacggcat tcaagattcg ggtctgcgcc acatccatat ggcgacaagg
     2101 gaacaaaaaa ccgatgaaac cgccccgacc caccagcgtt ggggaaactg ccaaaacatc
     2161 atcaggcagg atgcggtcat catgctgatg gcgaaaatct tggctttgcg cggcactgcg
     2221 ccgttttgtt cccagttatg aaccatcggg ccgaaatagc ggtgccggtg cagccagcgg
     2281 tgaaagcgcg gggatgcctt tgcccagcag gcggcggaga gtagtacgaa cggcgtggtc
     2341 ggcaacagcg gcaaaaaaat gccgatgatc cccagcagca gggagatgcc gccgcaggca
     2401 attaaaagat aacggatcat tttgaaatat ttttcttatt gtgcggataa gggcaggatg
     2461 tgataccgag ttttgcccag ccttcatgtc ccattttttc cagcagggcg atattgcgtt
     2521 cgaatattgc cgaagcgtcg ggaaaggctt gtgcggcttt ggcaatgctg tcttcgcgga
     2581 tgaggtgcag cgtcggatag ggagaacggt tggtgtagtt gccgatgccg tctgaatccg
     2641 tgccttcaaa ttggaaatag ggatgaaacg gggcgatttg gacgatgcct tccaagccgt
     2701 tttcgacaac ggcggcatcg gcaatgtcga gcatatcgtt gaatacgtcg aaatcgggga
     2761 atagggtcgg gtgaaccagc agggtggttt ccagttcggt ggcgggtgta ttgcccagtc
     2821 gctgcagttc ttcgtccaag tcttccaaaa aaccgtcaag gtgtttggct tcgctgatcg
     2881 cgatgcggac aaggttttta acgtgggggg ctttggcaaa gggacacagg ttcagaccga
     2941 tgacggcttt ttccaaccat tgtccggtgt gttcggcaac agcatcttta ttttcggaag
     3001 tatcgatatt cattattgtc atgtaaatgt gtttgcagag tgcacgtgcg ggaaaatcgg
     3061 gaagggcact attccttcag caggtggttg agcggcaggg aggtggtgtg tttgatttct
     3121 tttaaaacaa agctcgattg cgcatcctgt acgccgtggt gggataaaag cgtatccaaa
     3181 acaaaatggg aaaacgcgtt catatcggta aaaaatgcct gaagcaggta gtcggtctcg
     3241 ccggttaggg cgaagcagct caagacttcc ggccattttc gaaccgatgc ggcaaaatct
     3301 tcccgcgcgt cttttgcttt gcggatggaa acgcggataa atgcctgaag acctaagttg
     3361 acggattccg gagacagcag cgcggcatat tggcggacga tgccggcatc ttccaactgc
     3421 ttcagacggc gcaggcacgg agaaggcgaa agtgcgacac gttcggacag ttcgacattg
     3481 gtcagcctgc cgttttcctg gaggacctgt aagattttaa tatcggtttt gtctaaagtg
     3541 agttggggca tatttgcgtt ccgttttaag gaattcggat tgtctgtccg tatgtttgcg
     3601 gcaatccgca tagatggaga ccatattaac atataaaaag ttataccgtc atccgggaca
     3661 aattttgttt tcggaaaatc atgtgaaaac agaggcggtc ggtttgcatc tcttttaaga
     3721 cggcttgccc aaaccgccga ttcaagacat aatcgggaaa tgtgcaggag agtgttacac
     3781 ccaactacaa tgtaaccacc gaaggcgcag acacccttaa atcgctcagg tatcagggac
     3841 tgcacattga aacaaacaat ctggagagcg gcgttagaat aacgtccacc gaaggggaga
     3901 aggccgtctg aaccaccatt cagacaaccg cgcaaagcag tgagcagact ggtttgccat
     3961 catgcggata cggtcgaaaa tctcaggttc aaggacagat agggtcatcc gcgcacaggt
     4021 gcgcgggcgg catctgaaca aaaaatccgg agaaacttga gaatgactgc tctgaaaacc
     4081 accccgtttc atcaagccca tcaagatgca ggcgcgaagc cggtagattt tgccggctgg
     4141 gaactgccca tccattatgg ttcgcaaatc gccgaacacg aagccgtgcg caccgacgcc
     4201 ggtatgtttg acgtatccca tatgctcgtt accgacgtcg tcggagcgaa cgccaaagcc
     4261 tttttccgca aattgattgc caacgacgtt gccaaactcg cttttgtcgg caaagccctt
     4321 tattccgctt tgctcaacga caacggcggc gtgattgacg acttgatcgt ttaccgcacc
     4381 aacgaggccg aaacccaata ccgcatcgtg tccaacggtg cgacccgtga aaaagacacg
     4441 gcacaattcc acaaagtcgg acaagagttc ggcgttgcct tcaatccgcg ctacgacctc
     4501 ggtatgcttg ccgtacaagg tcctaaagcc attgaaaaac tcctgaccgt caaacccgaa
     4561 tgggcggatg tgattcacgg cctcaaaccg ttccaaggcg cggatttggg caacgactgg
     4621 tttgtcgccc gtacaggcta caccggcgaa gacggcgtcg aagtgattct gccggatacc
     4681 gaagccgtcg cattcttcaa agccctgcaa acagcaggcg tacagccttg cggcctcggc
     4741 gcgcgcgaca ccctgcgcat ggaagccggt atgaacctct acggcaacga tatggacgac
     4801 gacaccagcc cgctcgaagc aggcatgggc cggacggttg atttgaaaga cgaaagccgc
     4861 gattttgtcg gtaaagccgc cttgttggca ttgaaagaaa aaggcgttgc cgtcaaacaa
     4921 gtcggtttgc tgctcgaaaa aggcggcatc ctgcgcgcgc atatggaagt gttgaccgac
     4981 aaaggaaaag gcgaaaccac cagcggcgta ttctcgccca gcctgaaaca atctatcgcc
     5041 atcgcccgcg taccgaaaga ttttgacggc gataccgcca aagtcctgat tcgcggcaaa
     5101 gaagcggacg tgcgcgtact gaagctgccg ttcgtccgca gcggtcaaaa acagtttgat
     5161 taatatcggt tcagacggca tttgtttgaa ttaagccgtc tgaaagcagg ttttaaggaa
     5221 ttgttatttc ctatgaaagc aaattttggc agtttattgt tgttattgat gttatcggct
     5281 tgcgcttcgt cgccggcgag cgcggattga tgtttttcga tacagaagga aaatcccata
     5341 ctgcaatatt tatgaaaatc gttggcggca atggaaagat tgccggcgag gtggtaatag
     5401 acggggaata tgctcttgat attgtccgca tgaatgatga tttgagtatt gccgtgcaag
     5461 ataataaggg tagaagccgt gcttccgacc tggaagcatt gtcgcaagcg aaatcattta
     5521 ccctaattgc gttcagccgt tatgcgggca cagttgaagt ggataaagta tcggctgtta
     5581 caggcatttg tgcagattac cgcagcaaag gcctgaaggt tgccgttgca gacaatatga
     5641 ggcccaaaca agaaatcgtt gtattccgtg caagcggcgt aatggcaagt aataagcgca
     5701 agataaacga agcatatttg gattattctg catcctctaa actgccttcc gcccaaaagc
     5761 aagaattgga acaagataaa aacaatatga ttgccgtcca ttacggacgc ttggaagaat
     5821 taatcgcacg aggtatctgc gtacgctaag ttgtgcagat gccgtctgaa aataaccccc
     5881 taccaatgga gaatcaaacc atgagcaaca acatcccaac agaactgaaa tacgttgcca
     5941 gccatgaatg gctgcgcctt gaagaagacg gtaccattac cgtcggtatt acccatcacg
     6001 cgcaagagct gttgggcgac atcgtgtttg tcgagctgcc cgaagtcggt gcgaacctcg
     6061 ccgccgaaga acagtccggt gtggttgagt ctgtaaaagc cgcatccgac gtgtatgcgc
     6121 cgattgcggg cgaagtcgtt gccgtcaacg atgacttgcc aggcgcgccg gaaaccgcca
     6181 acagcgaccc ttacggcgca ggctggttct tcaaaatcaa acctgccaat cctgccgatt
     6241 acgacggtct gctgactgcc gaacaatacg cgggcgaagt ggattaaacc gcccgtttgt
     6301 ccgacggcaa ccgtcggaca aacggaaact gcaccttcag acggcatttc tgcggtcgga
     6361 ggtgcagttt ttttgtccgt gttttaagga tgcagttagg ctataataac ggtctatatc
     6421 catcttcacc gattttttca tgcaacttac cgctgtcgga ctcaatcatc aaaccgcacc
     6481 tttaagcata cgggaaaagc tggcgtttgc cgccgccgcc ctgccagaag ccgtccgcaa
     6541 tcttgcccga agcaatgcgg caacggaggc ggtaatcctt tctacctgca accgcaccga
     6601 gctttactgc gtcggcgatt cggaagaaat catccgatgg cttgccgatt accacagttt
     6661 gccgattgaa gaaatccgtc cgtatctgta cacgctggat atgcaggaaa ccgtgcgcca
     6721 cgccttccgc gttgcctgcg gcttggattc gatggttttg ggcgagccgc agattttggg
     6781 gcagattaaa gatgcggtgc gtgcggctca agaacaggaa agtatggggg caaaactcaa
     6841 tgccctgttc caaaaaacct tttccgttgc taaagaagtc cgtaccgata ccgctgtcgg
     6901 cgaaaattcg gtttcgatgg cttccgcgtc cgtcaagttg gcggaacaga tttttcccga
     6961 catcggcgat ttgaacgtat tgtttatcgg cgcaggcgaa atgattgagc tggttgccac
     7021 ttattttgcc gccaaaaatc cccggctgat gacggttgcc aaccggacgc tggcgcgtgc
     7081 acaggagttg tgcgacaagc tcggtgttaa cgccgaaccg tgcctgctgt ccgatctgcc
     7141 tgccattctg cacgattacg acgtggtggt ttcttcaacg gcgagccagc ttccgatagt
     7201 cggcaaaggc atggtcgaac gcgcattgaa acagcgtcag agtatgccgt tgttcatgct
     7261 tgacttggcc gtgccgcgcg atattgaagc ggaagtcggc gatttgaacg atgcgtatct
     7321 ttatacggtg gacgatatgg tcaacatcgt ccaaagcggc aaggaggcaa ggcagaaggc
     7381 cgccgccgcc gccgaaacgc tggtgtccga gaaggttgcc gaatttgtca ggcagcagca
     7441 gggcaggcag agcgttccgc tgattaaggc cttgcgggac gagggcgaga aagcgcgcaa
     7501 gcaggtgttg gaaaatgcga tgaaacagct tgccaaaggc gcaacggcgg aagaggtttt
     7561 ggaacggctg tccgtccaac tgaccaacaa gctgctgcat tcgccaactc aaaccttgaa
     7621 taaggcgggg gaagaagata aagatttggt tcatgccgtc gcgcagattt atcatttgga
     7681 caaataacgg tgcgccggaa aaggatgccg tctgaagggg tttcagacgg catatttttg
     7741 tgccgcctga caacatcgtg aaatcccaca ttatatcgat gtaattacaa agtattatcc
     7801 tatatgcgga ttggttttaa gatttgtaaa tttgatttgc atcaaaaatc gccgatagat
     7861 gattcatata atatcaatat taaagagtat cggtatatcg gggatagcta tgtcctgttt
     7921 ttcaatccgg cgtatgtctg cgtttcgggc gcggataacg gcgtttttta ccgcctttgt
     7981 ctttttgacg gcggcactgc ccgcttatgc ggagcgtctg cctgattttc tggcgaaaat
     8041 acagccttcg gaaatttttc cgggtgcgga ccgttacggc aagccggaag gcaagcctat
     8101 ggttgcccgc gtttacaaag gcgatgagca gctcggtttg gtttatatca cgaccgatgc
     8161 ggtcaatacg cgcggttatt cgagcaaacc gatcgatacg ctgatggctt tggcaaacga
     8221 cggcacgata gccggggcga aactggtcga tcatcacgaa ccgattatgc tgatcggtat
     8281 cccgcaatcg cgtgtcgata agttcatcga caaatatatc ggtctgaatt ttattaaaaa
     8341 tccgccgacc ccgagcgtgg cgccgggcga catcatcagc ggtgcgactg ttacactgat
     8401 ggtggttaac gacagcatcc agcgttcgta caaggtcatt gccaaccaat accgtctggg
     8461 ttcggacaag gcccttcaga cggcatccgc ttccgatgtt cgggaagccg cgcctgcgtc
     8521 agaaacccgt ccgcgccgta tggcaaatcc cgacaagcag gatattttgt cttgggacga
     8581 acttttgaaa caaaaggccg tcggccatct gcatatcacg cttgatcaaa tcaacaaact
     8641 gtttgagaaa ggcggcaagg ccggcgtggc cgatcacgcc gaacagggcg atcctgacga
     8701 tacctttatt gatttgtatg ttgccttggt cagccagcct tccatcggta aaagcctgct
     8761 gggtgaggac ggctgggcgc atctgcaaaa acggctgaaa cccgggcagc aggcggtttt
     8821 ggttgccgga gagggccgtt attcttggaa aggttcgggc tatgtgcgcg gcggtatttt
     8881 cgaccgtatc gagatgattc agggggagaa cagcttccgt tttaccgatg cccaacacga
     8941 acgcgtcgtc gagctgtctg ccgccgatgc gccgcgtttt aaagaagttt cttggtttac
     9001 catccctgaa ggcgtagcgt ttgacggtgc ggagccgtgg cggctgtaat tgatggttca
     9061 gcgcgtcttg agtgtgaacg ataaagcctt cgttaccgcc gatttggatt acgaactgcc
     9121 ccaagcttat tacgtcgatg atcctaaagc gccgcccgta gaaatcagcg cgcctgtcga
     9181 agcagtacct gccgcagcat ccgataccgc ttcagacggc attgccgaag atgcgtcagc
     9241 tgaaaacggt gtatccaacc aattatggaa acagatttgg aaagcgaaac aggggcagat
     9301 tgtcgtcgtc ggcattgcgc tgaccatcct gcttttggta ttcctgttcc aagattggat
     9361 tgtgcgttac gaaaaatggt acgaccgctt ccgctttgct ttcctaactt ttacgctctt
     9421 ctatatcggc tggtatgcac aggcgcagct ttcggtcgtc aatacgctga cgctgttttc
     9481 cgccatcctt accgaatttc attgggaatt cttcctgatg gatccgattg tgttcatcct
     9541 gtggctgttc acggctgcaa ccatgctgct gtggaacagg gggacgtttt gcggttggtt
     9601 gtgtccgttc ggctcattac aggaattgac taaccgcatt gccaaaaaac tgggtgtgaa
     9661 acagattacc gtaccgcata tgctgcatac ccgtttgaac gtcataaaat acctgatttt
     9721 cttcggtttt ttggcaattt cgctgtatga tttgggtacg gtggaaaaat ttgccgaagt
     9781 cgaaccgttc aaaactgcga ttatcttgaa attcatgtgc gattggtggt ttgtcgcctt
     9841 tgccgtggca cttttgattg cggggctgtt tatcgaacgc ttcttctgcc gttatctgtg
     9901 tcctttgggt gcgggcatcg cgctgccggg cagattccgc gtgttcgact ggctgcgccg
     9961 ctacaaaatg tgcggcaacc cctgtcaaat ctgtacgcac gaatgccccg ttcaggcaat
    10021 cgcgtccgag ggcgacatcc atccgaacga atgtatccag tgcctgcact gccaagtgat
    10081 gtaccaccac gatacgcgct gtccgcaagt tgtggcggca aataagaaaa aacaaaaaca
    10141 ggctgctgcc aaatcagggg aattggaaaa tgtctctaag cagccgcagg aacaggtcgt
    10201 ccgttttgtc aaacccgaaa cggcgcaatc tgaaaaataa gcatccgggg gaagcctgaa
    10261 gccaaagtgt caggcaggca gggtttcccc ggaaggataa taaaattttt ttcccatcgg
    10321 cttgccgtcc gaaaaccggt ttgccgtatt ttatccaagg agtgttgtat gtcagacgaa
    10381 aaattagaac aaaacggctt gagccgtcgt tcgttcttag gtacggccgc cgcttccggt
    10441 gcaggtattg ccggtgcggg cctgttgggt ttggcgggtt gctctaaaga cggcgaacag
    10501 gctgccgcta acgcttccgg cgcggctccc gtcgccaagg cgcaagggga atccaaaccc
    10561 ggccaactgt cttccgaagt cggtccgggc gaactcgatc aatattacgg tttcctttcc
    10621 ggcggccagt ccggcgagat gcgcctgatc ggtctgcctt ctatgcgcga actgatgcgt
    10681 atccccgtgt tcaatatgga cagcgcgacc ggttggggac gcaccaatga gagcttgaaa
    10741 gtcctcaacg gcaatattac cgaagaaacc cgtaaattct taaaagacgg cggcctgcgc
    10801 tgctacccca acggcgactt gcaccacccg cacctgtcgt ttaccgacca aacttatgac
    10861 ggccgctatg cctatgccaa cgacaaggca aacaaccgcg tctgccgcgt gcgtttggat
    10921 gtgatgaagg ccgacaaaat catcgacatc cctaatgatt caggtattca cggtctgcgt
    10981 ccgcaacgtt atccgaaaac cggttatgtt tttgccaacg gcgaacacat tacccctgtc
    11041 agcggtgtag gcaaactgga tgatgcgaaa acctggaatg cagtgtacac cgccatcgac
    11101 ggcgaaacca tggagatcgc atggcaagta ttggttgacg gtaacctgga caacggcgat
    11161 gccgactatc aaggcaaata ttcttttgcc acctgctaca actccgagcg cgcgctgacc
    11221 gtacaaggtg cgtcttccaa cgagcaggac tggtgcgtcg ttttcgacct gaaagccatc
    11281 gaagaaggca tcaaagcggg cgacttcaaa gaagttaacg gtgtgaaaat gctggacggc
    11341 cgcgctgagg ccaaatccaa atacacgcgt tatatccctg tgccgaactc tcctcacggc
    11401 tgtaacgcaa gccctgacgg taaatacatc atgcccaacg gtaaactgcc tccaaccgtt
    11461 accgtattgg atgtcagcaa actggacgat ttgttcgccg gcaaaatcaa agagcgcgat
    11521 gtggtcgtag ccgaaccgca actgggtctc ggcccgttac acactgcatt cgacggtcgc
    11581 ggcaatgctt atacgacatt gtttatcgac agccaaatgg tgaaatggaa tattgacgat
    11641 gcgatcaaag cctacaaagg cgagaaaatc gatccgatca aacaaaaact cgacgttcac
    11701 tatcaaccgg gtcacaacca tacgaccatg ggcgaaacca aagaagccga cggtcaatgg
    11761 ctggtgtcct tgaacaaatt ctccaaagac cgcttcttga atgccggtcc tttgaaaccc
    11821 gagtgcgacc aattgatcga catctccggc gacgaaatgc gtctggtaca cgacaacccg
    11881 acttttgccg aaccgcacga cttgtgtttg gttgccgcgt ccaaactgaa cccgggcaaa
    11941 acttgggacc gcaaagaccc gtggttctag caggatgctt tggaacaggc gaaaaaagac
    12001 ggtgtcgagc tggaaaaagc cgccaaagcc gtacgcgaag gcaacaaagt acgcgtgtat
    12061 atgacggccg tcgcgcctgc ttacagtatc ccgcagtttg aagtgaacca aggcgacgaa
    12121 gtaaccgttt acgttaccaa cgtcgaaacc atcgaggacc tgacccacgg cttcactttg
    12181 gaaggctgcg gcatcgctat ggaaatcggc ccgcaagcca cttcttccgt aaccttcaaa
    12241 gccgtccgcc cgggtgtgca ctggtactac tgccaatggt tctgccacgc attgcatatg
    12301 gaaatgtccg gtcagatgat tgttcatccc aaataatgcg gcccgacggc tgaatatgga
    12361 gaatggcgtc tgaaggcaac aggtcttcag acggcattta tagccaaacc gcttgccgta
    12421 ccgtttcagg cggtttcgct ataaaaacag gcacatttga atgatgcttt attttgttcg
    12481 gccataccga acgaaacaga gggacactat gcacacatcc gcgctccgta tatggctgaa
    12541 ggccgtgctg atacttgccg ccggcagcat ttttcagacg gcatcggcag ccgtcgtcca
    12601 cgtttccccg caagacaacc ttgccgaaat ttttgcccgc gcccgggcgg gcgatacgat
    12661 caaactggct tccggcgtat atcaaaccaa actttatatc gacaaaccga ttacgattga
    12721 agggcctgcc gaccgttccg caaccatcga aggggacaag agcgggcgta ccatagccgt
    12781 acacgcgccg gacgtaacgc tccgcaacct gaccgttacc cgttccggta tgagcctgcc
    12841 agcaatggat gccggtattt atctcgaaaa agccgccccg cgcgccctga ttgaacacaa
    12901 caatattttt gataattcgt tcggcgtata ccttcatggt tctgccgatg cgatggtgcg
    12961 cgagaataaa atcgtcggcg atgcgacctt gcgcgtgaat gagcgcggca atggcgttac
    13021 cgtttggaac gcgcccggcg cgcaggtcgt cggcaacgac atttccaaag ggcgggacgg
    13081 cattttttcc aataccagca cgcacaacac ctataaaaac aaccgcttca gcgacctgcg
    13141 tttcgccgtc cactatatgt acaccaacga cagcgaagtc agcggcaata tttgcgtggg
    13201 caacaatatg ggctatgtgc tgatgttttc cgaacggctc aaagtgttcg acaatatcgc
    13261 cgtcggcagc cgcgattagg gcatcatgct caactatgtc aactattccg atattcacga
    13321 caatattatc aacaaagcgg gcaagtgcgt ttttgcctac aatgccaact acgataaact
    13381 gtccgccaat cattttgaaa actgccaaat cggcatgcac tttaccgccg ccatcgaagg
    13441 cgcgtccctg cacgacaatt cctttatcaa caacggaagc caggtcaaat atgtcagtac
    13501 gcgctttctc gactggagcg agggcggaca cggcaactac tggagcgaca acagcccgtt
    13561 cgatttgaac ggcgacggct tcggagacag cgcgtaccgt cccgacggca tcatcgacca
    13621 aatcatctgg cgcgcgcccg tatcgcgcct cttgatgaac agtcccgcaa tcagcatcgt
    13681 caaatgggcg caggcgcagt ttcccgccgt tctgcccggc ggcgtggtgg acagcaaacc
    13741 gctgatgaag ccttatgccc ccaaaattca aacccgttat caggcgatga aggacgagtt
    13801 gctcaaagaa gccgaaacgc ggcagtcgga acggggcagg gcggaaaacg gttctttgaa
    13861 ctagtctgct tcagacggca cccggattca aatgccgtct gaaaacacaa aaggaacaac
    13921 catgaccaca catcatgtcg aattgaggaa ggtaaccaaa cggttcgggg cgcaaaaagc
    13981 cgtcagccaa gtcgatttgg ttttgaaggc aggagaaagc gtcgggcttg ccggacacaa
    14041 cggcgcgggc aagtccacca tcatgaagct gatactcggg ctgattaccc cgaccgaagg
    14101 cgaagtgatg cttttgggcg aacgtaccgg cagcaaagcg ggggcgcggc ttcgcagcca
    14161 aatcggctac ctgcccgaaa ccgttgcgct gcacccttcg ctgaccggca tcgaaacgct
    14221 ggatttttat gccaaactta aaaaacagcc gctcacgcag aaccgggggc tgcttgagcg
    14281 cgtcggcatt tcccagaccg cacaccgccg cgtcggcact tattctaaag ggatgcgcca
    14341 acgccttgcc ttggcacaag ccctgctggg cgagcccaaa gtcctgctgt ttgacgaacc
    14401 gacaaccggc cccgaccctg catcacgaca aatgttttac gaagtcgtgc gcgaactcaa
    14461 cgggcgcggc gcgaccgtat tgctcagtac ccacgccctt gccgagttgg acggacacgc
    14521 cgaccgcatt gtcgtgatga aaaacggcgt taaggttgcc gacggcagta tggacgaatt
    14581 gcacgtccaa agcgggctgc cgctgaccgt caatatccgc ctcaatgcgc cgcgcaccct
    14641 cagcagccgc tggcagccgc tttcagacgg catttcctac cgggcgcaat gtcaggccga
    14701 agaacgtatg gaactgttgg gcgaattggg cagcctgtcc gaccttgcct atttcgacat
    14761 ccacacgccg acgcttgacg aaatgtacgc acggttcttg aaaagggagg acgtatgaac
    14821 cccgtttgga ttattaccgg caaagaagcc cgcgacagcc tgcgtaaccg ctgggtgctt
    14881 gccgccgtcc tcctgcttgc cgcactcgcc ctttctttgg gtttcctcgg cagctcgccc
    14941 accggttcgg tcaaggtcga tccgctgacc gttaccgtcg tcagcctgtc gagcctgtct
    15001 attttcctga ttccgctgat tgcgatgctg ctttcttatg acgcactgat cggcgaaatc
    15061 gaacgcggta cgatggcgtt gctgttgagt tatcccattt ggcgcaacca aatccttgcc
    15121 ggcaagtttg tcggacacct catcatcctc gcccttgcca ccacggcagg ttacggattg
    15181 gcaggcatta cgctgcaact tgccaacggc ggtttcgaca tcgccgtttg gaaacccttt
    15241 gcgctgttga ttgccgccag cgtgatttta ggtgcggcat ttctgtctat gggctacttg
    15301 attagtgcaa aggtcaaaga gcgggggacg gcggccggta tttccatcgg cgtatggttg
    15361 tttttcgtcg tcatcttcga tatggcgctt ttgggtattc tggttgccga ctcgaaacag
    15421 gtcatcaccg cgcccgtcgt tgaaacggtg ctgctgttca atcccaccga catttaccgc
    15481 ctgctcaacc tgaccggtta cgaaaatacg gctatgtatg cgggtatggc gggtttgagc
    15541 ggacaaatcg gcctgaccgt ccccgttttg ctgaccgcgc aggttttatg ggttatcatt
    15601 ccgcttgttt tggcagccgg aatttttaga aagcgacgaa tatgaagaaa accctgttgg
    15661 caattgttgc cgttttcgcc ttaagtgcct gccggcaggc ggaagaggca ccgccgcctt
    15721 taccccggca gattagcgac cgttcggtcg gacactattg cagtatgaac ctgaccgaac
    15781 acaacggccc caaagcccag atttttttga acggcaaacc cgatcagccc gtttggttct
    15841 ccaccgtcaa gcagatgttc ggctatacca aactgcccga agagcccaaa ggcatccgcg
    15901 tgatttacgt taccgatatg ggcaatgtta ccgattggac gaatcctaat gccgacacgg
    15961 agtggataga tgcgaaaaaa gccttttacg tcatcgacag cggctttatc ggcggtatgg
    16021 gcgcggaaga cgcgctgccg ttcggcaaca aggagcaggc tgaaaaattt gcaaaggata
    16081 aaggcggcaa ggtcgtcggt tttgacgata tgcccgatgc ttacattttc aagtaaatcc
    16141 aaacagaaat gccgtctgaa cgttgaagcc aaggttcaga cggcattttt tgtcataggt
    16201 tcaaacgggg caggttcgaa caactgcccc attccttatt cctgaccggg cgtaacggcg
    16261 gaatgaggtt tacatcccgc cgtaattcgg tccgcttgcg ccttcggggc aaatccaagt
    16321 gatgttttgc gtcgggtctt tgatgtcgca ggttttgcag tgcacgcagt ttgccgcgtt
    16381 gatttgcagg cgcggactgc cgttttcttc gatgatttca tatacgccgg ccggacaata
    16441 gcgcgtttcg ggagaggcgt attctttata gttcacgtct atcatcgttt gcggattgtt
    16501 cagcaccaaa tggtcgggct ggttttcttc gtgcgcgaga ttggcaagga agacgctgct
    16561 caagcggtca aaggtcaaca caccgtcggg tttcggataa tcaatcggct tgcacacggc
    16621 ggcttttttg agctgctcgt tgtctttgcc gtgatgtttc aaggtccacg gggctttgcc
    16681 tctgaaaatc atctggtcga tgccggtata aagcgagccg aggtaaacgc cccatttgaa
    16741 tgacggacgg acattgcgcg cggcataaag ctcttgatac agccagcttt gttcaaacag
    16801 tttttgatag tttcccgctt ctttgccgct gtcgaagctt tccacttctt caaagttttc
    16861 caacaagggg aacacggctt cggcggcgag catggcggat ttcatcgcgg tatgaatgcc
    16921 tttgatgcgc ggcatattga ggaaccccgc cgcatcgccg attaaaacgc cccccttgaa
    16981 cgagagcttc ggcaggcttt gcaaaccgcc ttcaatcagc gaacgcgcgc cgtaagcaat
    17041 gcggcggccg ccttcaaagg ttttgcggat ttcgggatgg gttttgaaac gttggaactc
    17101 ttcaaacggc gacagatacg ggttttgata gtccaaaccg accacgaagc cgacggcgac
    17161 tttgttgtcg tcgaaatggt aaataaacgc accgccgtag gttttgctgt ccagcggcca
    17221 gcctgcgcta tgcaccacca aaccgggctg atgctgttca gacggcactt cccaaacttc
    17281 tttgatgccc aagccgtaag tttgcggctg gctgttttgg tcgagttgga aacgttcgat
    17341 gatttgcttg gaaagcgaac ctcggcaacc ttcggcaaac agggtttgcc gcgcccaaag
    17401 ctccatgccg ggctggaaac tgtcggtcgg cccgccgtct ttgcctacac ccatattgcc
    17461 ggtcgcaata cctttgaccg agccgtcttc gtgatacagc acttcggcgg cggcaaagcc
    17521 cggatagatt tccacgccca tattttccgc ctgctccgcc aaccagcgca cgacttcgcc
    17581 taagctgacg atgtagttgc cgtgattgtc gaaattcggg gtaataggca gattgaacgc
    17641 ttttttctcc gtcaggaaca acaccttgtc ctgagttacc gtgcgcgtca gcggcgcgcc
    17701 tttttctttc cagtcgggaa tcagctcatt cagcgcaatc ggatcgataa ccgcgcccgc
    17761 cagcgaatgc gcccccgcct ccgaaccctt ctccgccacg caaacgctga tttcgcgccc
    17821 gttctgttcg gcaagctgct tgagtttgat ggcggcggac aaccccgacg ggcctgcgcc
    17881 gacaatcacg acatcgtatt gcatactgtc gcgggtgatg gattctgtca tggcggttcc
    17941 tgtgtattta ttattgaatt gcaaatccgt aattatacaa cgggaacata tagttaccaa
    18001 atacaacaaa ggtcgtctga aaaccatatt ttcggttttc agacgacctt tgtcgaaatt
    18061 tcaataagca cgccaccatt ttacctgtcc gaccgcaaac tccgtctgac gtttcggact
    18121 gcgtgtgaaa aacgccttat ccccaccggc atccctccct ttcggcacga ccgccaaaat
    18181 tttgcctgcc aaatttccct cacgggtttg ccaagcctcc aaaaactgcg ccctgctcat
    18241 cgaaacatga cccggcgacg agtcggcaag caaaaccgta ttgccgccta tgccgcgcaa
    18301 taccgaaaaa tggtcgtctt tgcggtattt cagatacacg atgacgggga ttttcaactg
    18361 cgcgagctgc tcgaaagaca gggcatagcc cttcgcctca aaacccaaat cgggcataat
    18421 gcgccgcata tcctcaaacg acgcgcgcat ctgttcctta cccagttttt ccaacacttc
    18481 ttcttccgtc agcttttgcc cgtaaaaatt gttcaaaagc gtcgccaccg aagccgcccc
    18541 gcaggaaaaa tccaaatcct gctttacaat attgaaatcc cgccgcgctt tccaactctg
    18601 caatttgatt tttccgtaaa caacaggatt atcgttaaac atcggtgcag cattcaaacg
    18661 ataagacaag gg
//
LOCUS       DAXFZR010000045        18466 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000045 DAXFZR010000000
VERSION     DAXFZR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18466
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.045"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            49..124
                     /locus_tag="ACFJAS_001298"
     tRNA            49..124
                     /locus_tag="ACFJAS_001298"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Trp,seq:cca)
     gene            239..517
                     /gene="secE"
                     /locus_tag="ACFJAS_001299"
     CDS             239..517
                     /gene="secE"
                     /locus_tag="ACFJAS_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC3982540.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            519..1055
                     /gene="nusG"
                     /locus_tag="ACFJAS_001300"
     CDS             519..1055
                     /gene="nusG"
                     /locus_tag="ACFJAS_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC3982541.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1156..1590
                     /gene="rplK"
                     /locus_tag="ACFJAS_001301"
     CDS             1156..1590
                     /gene="rplK"
                     /locus_tag="ACFJAS_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC3982542.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1590..2285
                     /gene="rplA"
                     /locus_tag="ACFJAS_001302"
     CDS             1590..2285
                     /gene="rplA"
                     /locus_tag="ACFJAS_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC3982543.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            2513..3013
                     /gene="rplJ"
                     /locus_tag="ACFJAS_001303"
     CDS             2513..3013
                     /gene="rplJ"
                     /locus_tag="ACFJAS_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC3982544.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3071..3442
                     /gene="rplL"
                     /locus_tag="ACFJAS_001304"
     CDS             3071..3442
                     /gene="rplL"
                     /locus_tag="ACFJAS_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC3982545.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            3633..7811
                     /gene="rpoB"
                     /locus_tag="ACFJAS_001305"
     CDS             3633..7811
                     /gene="rpoB"
                     /locus_tag="ACFJAS_001305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC3982546.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(7776..7916)
                     /locus_tag="ACFJAS_001306"
     CDS             complement(7776..7916)
                     /locus_tag="ACFJAS_001306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982547.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            7964..12139
                     /gene="rpoC"
                     /locus_tag="ACFJAS_001307"
     CDS             7964..12139
                     /gene="rpoC"
                     /locus_tag="ACFJAS_001307"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC3982548.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDMMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12427..12837
                     /locus_tag="ACFJAS_001308"
     CDS             12427..12837
                     /locus_tag="ACFJAS_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982549.1"
                     /translation="MKVSQSAMNKLVIAVIASMGLTACAGGGSDSSMSVQPSVSEQLK
                     DNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGESVK
                     AMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGG"
     gene            12797..13489
                     /locus_tag="ACFJAS_001309"
     CDS             12797..13489
                     /locus_tag="ACFJAS_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HFC3982550.1"
                     /translation="MLMVKNTKVVRGGELDVLPKGLSEQKIEFYGADKEQNYALLKTW
                     VYEQPYSVVRGYFGYSRKDSNPIEGDGQNPEEIPFDLYLGDIRGVATDEDKLPKAGSF
                     QYEGRAFGGNGVLSKESLDNHNGVFRYTIDFDRRKGSGSIEGMEQYGKIKLEEAAIER
                     IPYRESGSSLGLKDRVSYFGVNEGVAMLEKDNEIKKYHLGIFGEAANEVAGAVSQEHK
                     HQAVIGFGGEKK"
     gene            13729..13971
                     /locus_tag="ACFJAS_001310"
     CDS             13729..13971
                     /locus_tag="ACFJAS_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982551.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            14168..15028
                     /locus_tag="ACFJAS_001311"
                     /pseudo
     CDS             14168..15028
                     /locus_tag="ACFJAS_001311"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            15207..15578
                     /gene="rpsL"
                     /locus_tag="ACFJAS_001312"
     CDS             15207..15578
                     /gene="rpsL"
                     /locus_tag="ACFJAS_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC3982552.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            15696..16166
                     /gene="rpsG"
                     /locus_tag="ACFJAS_001313"
     CDS             15696..16166
                     /gene="rpsG"
                     /locus_tag="ACFJAS_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC3982553.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            16185..18290
                     /gene="fusA"
                     /locus_tag="ACFJAS_001314"
     CDS             16185..18290
                     /gene="fusA"
                     /locus_tag="ACFJAS_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC3982554.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            18378..>18466
                     /locus_tag="ACFJAS_001315"
     CDS             18378..>18466
                     /locus_tag="ACFJAS_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3982555.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTA"
BASE COUNT         4983 a         4013 c         4787 g         4683 t
ORIGIN      
        1 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aagtttagag gccaatagct
       61 caattggtag agtatcggtc tccaaaaccg agggttgggg gttcgagacc ctcttggcct
      121 gccaaataaa aaattaaccg gccttgtgcc ggttaatttt tttgtatttg ttatttagta
      181 aactctcttg ccatttacat ggattgagaa tagacagatg ctatgatgga taaataatat
      241 gacagaacat acacctgaaa aaaagaatgt taaagtgaat caactggttg ttcaagataa
      301 agaatctgca tctaattccg gtaaggaggg tttttttgca tatttctcaa attcttggtc
      361 cgaattcaaa aaggtggttt ggcctaagcg tgaagatgcc gtcagaatga ctgtatttgt
      421 tatagtgttt gttgctgtgc tttctatatt tatctatgcg gcagatacag caatttcgtg
      481 gttatttttt gatgtattgc tgagaaggga aggttgagat gtcgaaaaaa tggtatgttg
      541 tacaggcgta ttcggggttt gagaagaatg tccaacgaat attggaagag cgaattgccc
      601 gtgaggagat gggagattat ttcggacaaa ttctggtgcc tgtagagaaa gttgttgata
      661 tccgcaatgg tcgtaagact attagtgaaa gaaagtcata tcctggttat gtgttagttg
      721 agatggaaat gacagatgac tcttggcatc ttgtaaaaag cactccccgt gtttctggtt
      781 ttattggagg gagggctaat agacccacgc cgattagtca gagagaggct gaaattattt
      841 tacagcaggt tcagaccggc atagagaagc cgaaaccaaa agttgaattt gaggtcggtc
      901 aacaggttcg tgtaaatgaa ggaccgtttg cggattttaa cggggtggtt gaggaggtca
      961 attatgaacg gaataagtta cgcgtgtctg ttcagatatt tggtagagaa acacccgttg
     1021 agctggagtt cagccaggtt gagaagatta actgattttt atacttgaaa aaaaagcaat
     1081 aagaggatag aatcaaaaac taacttgggg agcggaaatg gttccgcgtc ttacccgttt
     1141 ttaggagtcc gttaagtggc aaagaaaatt atcggctata ttaaactgca aattcctgca
     1201 ggcaaagcca atccatcccc cccagttggt cctgctttgg gtcaacgtgg tttgaatatc
     1261 atggaattct gtaaagcatt taatgctgca acccaaggta tggaatctgg tttgccaatt
     1321 ccggttgtga ttactgcatt tgcagataaa tcattcacat ttgtgatgaa aactcctccg
     1381 gcttctattc tgttgaaaaa agccgccggt ttgcaaaaag gtagttctaa tcctctgaca
     1441 aacaaggtag gtaaattgac ccgtgcccag ttggaagaaa ttgctaaaac taaagagcct
     1501 gatttgactg ctgctgactt ggatgcggct gttcgtacta tagcaggttc tgctcgctca
     1561 atgggcttgg acgtggaggg tgttgtataa tggctaaagt atctaaacgc ttgaaagttc
     1621 ttcgctcttc tgttgaagct aacaaactgt acgcaatcga cgaagcaatt gccttggtta
     1681 aaaaagctgc taccgctaaa tttgacgaat ctgttgacgt atctttcaac ttgggcgttg
     1741 attctcgtaa atctgaccaa gttatccgtg gttcagtcgt tctgcctaaa ggtactggta
     1801 agacaacccg tgtggctgta tttactcaag gtgtaaatgc agaagctgct aaagaggctg
     1861 gtgcggatgt cgtcggtttc gaagatttgg ctgctgaaat caaagcaggc aatctgaact
     1921 ttgatgttgt tattgcttct cctgatgcaa tgcgtatcgt tggtcaattg ggtaccatct
     1981 tgggtcctcg tggtctgatg ccaaacccta aaataggtac ggttacccct aatgttgccg
     2041 aagcggtaaa aaatgcaaaa gcaggtcaag tgcagtaccg taccgacaag gcaggtattg
     2101 ttcatgcaac gattggtcgt gcttcattcg ctgaagctga tttgaaagag aactttgatg
     2161 cgttgctgga cgctatcgtt aaagccaaac ctgctgctgc taaaggtcag tacctgaaaa
     2221 aagttgctgt atccagcact atgggtttag gtgttcgcgt tgatacatca agcgtgaata
     2281 actaatctaa gaaattttca agcaactctg tttttgagtt gcttgaattt gggctactta
     2341 aaattaagta gatgtccaag accgtaggga tcgtaagatt taatcgtaac tgccctacgc
     2401 agacggtagt cctgaaacac attgcaagat tgcttgtaag atgtcttttt aggttaccgc
     2461 gctggtggga tatcgttttg gtatcctgtt tataaacagt gggaggtaga ccttgagtct
     2521 caatattgaa accaagaaag tggcggtcga ggaaattagc gcggcaattg ctaatgctca
     2581 aaccctcgta gtcgctgaat atcgcggtat cagtgtttcc agtatgactg agcttcgtgc
     2641 gaatgcacgt aaagaaggcg tttatttgcg cgttctgaaa aatactctgg ctcgtcgcgc
     2701 agtgcaaggt acttcatttg tagaattggc cgatcaaatg gttggtccgt tggtttacgc
     2761 tgcttctgaa gatgctgttg ctgctgctaa agtgttgcac caattcgcga aaaaagatga
     2821 caaaattgtc gttaaagccg gttcttacaa tggcgaagta atgaatgctg ctcaggttgc
     2881 tgagttggct tctattccga gccgcgaaga gctgttgtcc aaactgttgt tcgttatgca
     2941 agctcctgta tcgggctttg cgcgcggttt ggctgctttg gcagagaaaa aagccggcga
     3001 agaagctgct taatcgattt tgtttctgtt aatcaattat tttttaatac aatatttgga
     3061 gtaaaatagc atggctatta ctaaagaaga cattttggaa gcagttggtt ctttgaccgt
     3121 aatggaattg aatgacctgg ttaaagcttt tgaagaaaaa ttcggtgttt ctgctgctgc
     3181 tgttgcagtt gcaggtcctg ctggtgccgg tgctgccgat gctgaagaaa aaaccgaatt
     3241 tgatgtcgtt ttggcttctg ccggcgatca aaaagtcggc gtgattaaag ttgtccgtgc
     3301 aattactggt ttgggtctga aagaagctaa agacatcgtt gacggcgcac ctaaaaccat
     3361 taaagagggt gtttctaaag ctgaagccga agacatccaa aaacaactgg aagcagcagg
     3421 cgctaaagtc gaaatcaaat aatttgatgc ttcttatgaa ggctggcagt tttctgccag
     3481 ccttattttg cttcttaaaa taagcattaa gtattgttta catttatttg catagttttt
     3541 atcaaatcat tgcaaataaa tgtaaatatc agattgatgc gtaccgttgt tttcagacgg
     3601 cctattattg aaaattactt ttcggagtgt gtatgaacta ttcgtttacc gagaaaaaac
     3661 gtatccgtaa gagttttgca aagcgggaaa atgttttgga agttcctttt ctactggcaa
     3721 cccaaattga ttcttatgcg aaatttttgc agctggaaaa tgcttttgac aaacgtaccg
     3781 atgacggtct gcaagcggca tttaattcta ttttcccgat tgtgagccac aacggttatg
     3841 ctcgtctgga gtttgtatat tacacgctgg gcgagccttt gttcgatatc cctgaatgtc
     3901 aattgcgcgg aatcacttat gctgcccctc tgcgcgcgcg tatccgtttg gtgattttgg
     3961 ataaggaagc gtctaaaccg acggtaaaag aagttcgtga aaacgaagtg tatatgggcg
     4021 aaattccgtt gatgaccccg agcggttctt ttgtgattaa cggcacagag cgtgtgattg
     4081 tttcccagtt gcaccgttcg cccggtgtgt tcttcgagca tgacaaaggt aagacacact
     4141 cttccggcaa attgttattc tccgcccgca tcattcccta ccgtggttca tggttggatt
     4201 ttgaatttga tccgaaagat ttgctgtatt tccgtatcga ccgccgccgt aaaatgccgg
     4261 taacgatttt gttgaaggct ttgggctaca acaatgagca aatcttggat attttctacg
     4321 acaaagaaac gttttatttg tcttcaaacg gtgttcaaac cgatttggtc gcaggtcgtc
     4381 tgaaaggcga aactgccaag gtcgatatct tggataaaga aggcaatgta ttggttgcca
     4441 aaggtaagcg cattactgcg aaaaatatcc gtgatattac caatgcaggc ctgacccgtt
     4501 tggatgtaga acaggaaagc ctgctgggca aagcattggc tgccgatttg attgattcgg
     4561 aaaccggcga ggtattggct tctgccaatg atgaaattac agaagagttg ttggccaaat
     4621 ttgatattaa cggcgtaaaa gaaattacga ccctttacat caatgagttg gatcagggtg
     4681 cttatatttc caataccttg cgcacggatg agactgccgg ccggcaggcg gcgcgtgttg
     4741 cgatttaccg tatgatgcgt ccgggcgaac cgcccaccga agaggcggtc gagcaattgt
     4801 ttaaccgctt gttcttcagt gaagacagct acgatttgtc ccgcgtaggc cgtatgaaat
     4861 ttaatacgcg cacatacgaa caaaaactgt ccgaagccca acaaaactct tggtacggcc
     4921 gcctgttgaa cgagacgttt gccggtgctg ccgacaaagg cggttatgtc ctgagcgtcg
     4981 aagatattgt cgcctcgatt gcgactttgg tcgagttgcg taacggccat ggcgaagtgg
     5041 acgatatcga tcacttgggc aaccgtcgag tacggtcggt aggcgagctg actgaaaacc
     5101 aattccgcag cggtttggct cgtgtggaac gtgccgtaaa agaacgtttg aatcaggcgg
     5161 aatcagaaaa cttgatgccg cacgacttga ttaatgcgaa acccgtttct gccgccatca
     5221 aagaattctt cggctccagc caattgagtc agtttatgga tcagaccaat cccttgtctg
     5281 aagtaaccca taaacgccgt gtatctgcat tgggcccggg cggtttgacc cgcgaacgtg
     5341 ccggcttcga agtgcgggac gtgcatccga cccactatgg acgtgtatgt cctattgaaa
     5401 cgcctgaagg tccgaacatc ggtttgatca actcattgtc tgtttacgcg cgcaccaatg
     5461 attacggttt cttggaaacg ccttaccgcc gcgttattga cggcaaagta accgaggaaa
     5521 ttgattactt gtctgccatc gaagaaggcc gctatgtgat tgcacaggcg aatgccgatt
     5581 tggattcaga cggcaatctg attggcgatt tggttacctg tcgtgaaaaa ggcgaaacca
     5641 ttatggcaac gcccgaccgc gtccaatata tggacgtggc aaccggtcag gtggtatccg
     5701 ttgcggcatc cctgattccg ttcttggaac acgatgacgc gaaccgcgca ttgatgggtg
     5761 ccaacatgca acgtcaggca gtgccttgtc tgcgtcctga aaaaccgatg gtcggtaccg
     5821 gtatcgagcg ttccgttgcc gttgactctg ctactgcaat cgttgcccgc cgaggcggcg
     5881 tggtcgagta tgtcgatgcc aaccgcgttg tgatccgtgt ccatgacgac gaagcgactg
     5941 ccggcgaagt gggtgtcgat atttacaatt tggttaaatt cacccgttcc aatcaatcta
     6001 ccaacatcaa ccaacgtccg gctgtcaaag caggcgatgt tttgcaacgc ggcgatttgg
     6061 tggccgacgg cgcgtccacc gatttgggcg aattggcttt gggtcaaaat atgaccatcg
     6121 ccttcatgcc gtggaacggt tacaactacg aagactcgat tctgatttcc gaaaaagtgg
     6181 ctgcggacga ccgctatact tcgattcaca ttgaggaatt gaatgtcgtt gcccgcgata
     6241 ccaagctggg tgcggaagac attacccgcg atattccgaa cttgtccgag cgtatgcaaa
     6301 accgtttgga cgaatccggt atcgtttaca tcggtgcgga agtagaagcc ggcgatgtgt
     6361 tggtaggcaa ggtaacgcct aaaggcgaaa cccagctgac gccggaagaa aaactgctgc
     6421 gcgccatctt cggcgaaaaa gcgtctgacg taaaagatac ttcattgcgt atgcctaccg
     6481 gtatgagcgg tacggttatc gacgttcaag tctttacccg cgaaggcatc caacgcgaca
     6541 aacgcgccca atccattatc gattccgaat tgaaacgcta ccgtttggat ttgaacgacc
     6601 aattgcgtat tttcgacaac gacgcattcg accgtatcga gcgtatgatt gtcggtcaga
     6661 aagccaacgg cggcccgatg aagctggcca aaggcagcga aatcacgacc gaatatctgg
     6721 cgggtctgcc gagcaggcac gattggttcg atatccgttt gaccgatgaa gatttggcca
     6781 agcagttgga actgattaaa ttgagcctgc aacaaaaacg cgaagaagcg gatgagttgt
     6841 atgagatcaa gaagaaaaaa ctgacccaag gcgacgagct gcaacccggc gtacaaaaaa
     6901 tggtgaaagt ctttatcgcc atcaaacgcc gtctgcaagc cggtgacaaa atggcgggcc
     6961 gccacggtaa taaaggtgtg gtatctcgca ttctgcctgt ggaagacatg ccgtacatgg
     7021 cggacggccg tcctgtggac atcgtactga acccgttggg cgtaccttcc cgtatgaaca
     7081 tcggtcagat tttggaagtt cacttgggtt gggcggcaaa aggtatcggc gagcgcatcg
     7141 accgtatgct gaaagagcga cgcaaagccg gcgagctgcg cgagttcttg aacaaactct
     7201 acaacggcag cggtaagaaa gaagatttgg acagcctgac tgatgaagaa atcatcgaat
     7261 tggcttccaa tctgcgtaaa ggtgcatctt tcgcctctcc tgtattcgac ggtgcgaaag
     7321 agtctgaaat ccgcgaaatg ttgaacttgg cttacccaag cgaagatcct gaggttgaga
     7381 aactgggctt caacgacagt aaaactcaaa tcacgctgta tgacggccgt tcaggcgaag
     7441 catttgaccg caaggttaca gtcggtgtga tgcactatct gaaactgcac cacttggttg
     7501 acgaaaaaat gcacgcgcgt tctaccggcc cgtacagtct ggttactcaa caacctctgg
     7561 gcggtaaagc tcagtttggc ggccaacgtt tcggtgagat ggaggtttgg gcattggaag
     7621 catacggcgc ggcatacacg ctgcaagaga tgctgactgt gaagtctgac gacgtgaacg
     7681 gccgtaccaa aatgtacgaa aacatcgtca aaggcgaaca caaaatcgat gccggtatgc
     7741 ccgagtcctt caacgtattg gtcaaagaga ttcgctcact gggtttggat atcgatttgg
     7801 aacgctactg atacgggttt cagacggcat aaggggagct gttctgcagg tatgcggggc
     7861 agcccgacaa tgtttaaaaa cgaaatgccg tctgaaagca ctgtaccttt atccatatcg
     7921 aaaatccgcc acgcggtaaa aatatttcct tcaaggagca gaaatgaatt tgttgaactt
     7981 atttaatccg ttgcaaaccg caggtatgga agaagagttt gatgccatca aaatcggtat
     8041 tgcctctccc gaaaccatcc gctcatggtc ttatggcgaa gttaaaaaac ctgaaaccat
     8101 caactaccgt acgttcaaac ccgagcgcga cggcttgttc tgcgccaaaa tctttggccc
     8161 ggtcaaagac tacgaatgct tgtgcggaaa atacaaacga ttgaaattta aaggcgtaac
     8221 ctgtgaaaaa tgtggcgtag aagttaccct gtccaaagtg cgccgcgaac gcatgggcca
     8281 tatcgaattg gctgcacccg ttgcccatat ttggttcttg aaatccctgc cttcccgctt
     8341 gggtatggta ttgaacatga cgttgcgtga tatcgaacgc gtattgtact ttgaagcatt
     8401 cgtggtgacc gatcccggca tgactccgtt gcaacgtcgt caattgctga ctgaagacga
     8461 ttactacaac aaactggacg aatacggcga cgacttcgat gccaaaatgg gtgcggaagg
     8521 tatccgcgaa ttgttgcgaa ccttggatgt agcaggcgaa atcgaaatcc tgcgccaaga
     8581 gcttgaatcg accggttctg acaccaaaat caaaaaaatc gctaaacgct tgaaagtatt
     8641 ggaagccttc catcgttccg gtatgaagct ggagtggatg attatggatg tgctgccggt
     8701 attgccgcct gatttgcgtc cgttggttcc gttggacggc ggccgttttg ccacttccga
     8761 tttgaacgat ttgtaccgcc gcgtcatcaa ccgcaacaac cgtctgaaac gcctgctgga
     8821 actgcatgcg cctgacatca tcgtccgcaa cgaaaaacgt atgttgcaag aagcggttga
     8881 ctcgctgttg gataacggcc gtcgcggtaa agccatgacc ggagccaaca aacgtccgct
     8941 gaaatcgttg gcagatatga ttaaaggtaa aggcggccgc ttccgtcaaa acctgttggg
     9001 caaacgtgtg gactactccg gccgttccgt gattaccgta ggcccatacc tgcgtctgca
     9061 ccaatgcggt ttgccgaaaa aaatggcgtt ggaactgttc aaaccattta ttttccacaa
     9121 attggaaaaa caaggtttgg cgtctaccgt taaagctgcg aaaaaattgg tagaacaaga
     9181 agtacctgaa gtatgggata tcttggaaga agtcatccgc gaacatccga ttatgttgaa
     9241 ccgtgcgccg accctgcacc gtttgggtat tcaagcgttc gagcctattc tgatcgaagg
     9301 taaagcgatt cagttgcacc cattggtgtg tgccgcattt aatgccgact ttgacggtga
     9361 ccaaatggca gtacacgttc cattgagctt ggaagcgcaa atggaagcac gcacgctgat
     9421 gctggcttca aacaacgtat tgtctcctgc caacggcgaa ccgattatcg taccttccca
     9481 agacatcgta ttgggtctgt actacatgac ccgcgaccgt atcaatgcca aaggcgaggg
     9541 cagcctgttt gccgatgtga aagaagtgca tcgtgcatac cataccaaac aggtcgagct
     9601 gggtacaaaa atcaccgtac gtctgcgcga atgggtgaaa aacgaagcag gtgagtttga
     9661 gcctgtcgtt aaccgttacg aaacaaccgt cggccgtgca ttgttgagcg aaatcctgcc
     9721 gaaaggtctg ccgtttgaat atgtcaacaa agcgttgaag aaaaaagaaa tttccaagct
     9781 gattaacgca tcgttccgcc tgtgcggctt gcgcgatacg gttatcttcg ccgaccacct
     9841 gatgtacacc ggtttcggat ttgcggcaaa aggcggtatt tccattgccg ttgacgatat
     9901 ggaaattcca aaagaaaaag cggccttgct ggctgaagcc aatgccgaag ttaaagaaat
     9961 cgaagaccaa taccgtcaag gtttggttac caacggcgaa cgctacaaca aagtggtcga
    10021 tatttggggt cgtgccggcg ataagattgc taaagcgatg atggacaacc tgtccaaaca
    10081 aaaagttatc gaccgtgacg gcaacgaagt cgatcaagag tcattcaact ccatttatat
    10141 gatggcggac tccggtgccc gtggttctgc ggctcagatt aaacagttgt ccggtatgcg
    10201 cggtttgatg gcgaaacctg acggatcgat tattgaaacg ccgattacct caaacttccg
    10261 cgaaggtctg accgtattgc aatactttat tgcgacccac ggtgcgcgta agggtttggc
    10321 ggataccgcg ttgaaaaccg cgaactccgg ttacctgacc cgtcgtctgg tggatgtaac
    10381 tcaagacttg gtcgttgttg aagacgattg cggtacttca gacggctttg tcatgaaggc
    10441 agtggtacaa ggcggtgatg tgattgaagc attgcgcgat cgtattttgg gtcgtgttac
    10501 cgcgtctgac gttgtcgatc cgtcaagcgg cgagactttg gttgaagccg gtacgttgtt
    10561 gactgaaaaa ctggtggata tgattgacca atccggtgtc gatgaagtca aagtccgtac
    10621 tccgattact tgtaaaaccc gccacggtct gtgcgcgcac tgttacggcc gcgacttggc
    10681 gcgcggcaaa ctggtcaatg ccggcgaggc agtcggcgtg attgcggctc agtctattgg
    10741 tgaaccgggt acccagttga ccatgcgtac gttccacatc ggtggtgcgg catcccgtgc
    10801 ggcagcagcc agccaagtcg aagccaaatc caacggtacg gcacgtttca gcagccaaat
    10861 gcgttatgtt gccaacaaca aaggcgagtt ggttgtcatc ggccgttctt gcgaagtcgt
    10921 gattcacgat gacatcggcc gtgaacgcga acgccacaaa gtaccttacg gtgccatcct
    10981 gctggtacaa gacggtatgg ccattaaagc cggtcaaacc ttggcaactt gggatccgca
    11041 tacccgtccg atgattaccg aacatgcagg tatggtgaaa ttcgagaaca tggaagaggg
    11101 cgttaccgtt gcaaaacaaa ccgatgatgt aaccggtttg tccactttgg tagtgattga
    11161 cggtaaacgc cgttcctcta gtgcttccaa actgctgcgt ccgactgtga aactcttgga
    11221 cgaaaacggc gtggaaatct gtattcccgg tacgtccact ccggtttcga tggcattccc
    11281 cgttggtgcg gtgattaccg tacgcgaagg tcaggaaatc ggtaaaggcg acgtattggc
    11341 gcgtattccg caagcctctt ccaaaacccg cgacattacc ggcggtctgc cgcgcgttgc
    11401 cgagctgttt gaagcacgcg tgccgaaaga tgccggtatg ctggcggaaa ttaccggtac
    11461 cgtttccttc ggtaaggaga ccaaaggcaa acaacgtctg attatcactg acgtggacgg
    11521 tgtagcatac gagactttga tttccaaaga gaaacaaatt ctggtacatg acggtcaagt
    11581 ggtaaaccga ggcgaaacca ttgtggacgg cgcggtcgat ccgcacgata ttctgcgtct
    11641 gcaaggtatc gaagcactgg cacgctacat tgtccaagag gtgcaagagg tttaccgcct
    11701 gcaaggtgtg aagatttccg ataaacacat cgaagtcatc atccgtcaaa tgttgcgccg
    11761 tgtgaacatt gcggatgccg gcgaaaccgg gtttattacc ggagagcagg tcgaacgcgg
    11821 cgatatgatg gcggccaatg aaaaagcttt ggaagaaggc aaagagccgg cacgttacga
    11881 aaacatattg ctgggtatta ccaaagcctc cctgtccacc gacagcttca tttctgccgc
    11941 atcgttccaa gaaaccaccc gtgtgttgac cgaagctgcc atcatgggca aacaagacga
    12001 actgcgcggt ctgaaagaga atgtcatcgt cggtcgcctg attcctgccg gtaccggttt
    12061 gacttatcac cgcagccgtc atcagcaatg gcaaggggta gagcaggaga ctgctgaaac
    12121 ccaagtaacg gatgaataat cttcggcgcg ttcattcaat aaaaaaccgc aagccttgag
    12181 cttgcggttt ttctttgtcc gattaaggca aaaacaagcg ttttcgtcat tttgaggcgt
    12241 gtggatataa gcccgataag tgtcccatat cttcgtgccg tcaaattatg catcgaatag
    12301 aacgagttaa atccgaaggt tattgtgtta aatattgcaa aaatacttta aaaatttcgc
    12361 acggtggggg gtagaattcc cagacttaaa gcttaagttt ttttaacaaa gtttggggga
    12421 gtctaaatga aagtatctca atcagcaatg aataaacttg ttattgctgt tattgcttct
    12481 atggggctaa cagcgtgcgc gggtggcggt tccgattcat ctatgtcggt tcaaccaagc
    12541 gtttcggaac aactgaaaga caatgcaaat gttgatgcga aagatgaaaa agttattgaa
    12601 tatctgaaga aatcatcatt aaaagatgta ccaaaagaat tgcaagccaa agtattaaag
    12661 gttaaaggtg atgaatatac aggagtgcga aaacaatatg caggtaagct cggtaagggt
    12721 gaatctgtca aggcaatgct atttctagac ggcgaagagc cgtttagtaa ggaacaactt
    12781 cagaagatgg atgtatatgt taatggtaaa aaatacgaag gtagtaaggg ggggtgaact
    12841 agatgttctc cccaaaggtt tgagtgagca aaagattgag ttttacggtg cggataaaga
    12901 acagaattat gcgctattaa aaacttgggt ttatgagcaa ccgtattctg tggttagagg
    12961 ctattttggc tacagtcgaa aagacagtaa tcctatagaa ggggatggac aaaatcctga
    13021 agagattcct tttgacttgt atctgggcga cattagaggc gtggcaacag atgaagacaa
    13081 attacccaag gctgggagct ttcaatatga aggtcgggca tttggcggta atggggtttt
    13141 atccaaagaa tcgttagata accataatgg cgtatttaga tacacaattg attttgatcg
    13201 tagaaaaggc tctggctcaa ttgaaggaat ggaacaatat ggcaagatca aattagaaga
    13261 agctgctatt gagagaatcc cttatcgaga gtccggctcc agcttggggt tgaaagaccg
    13321 ggtttcttat ttcggcgtga acgaaggggt ggctatgtta gagaaggata atgagattaa
    13381 aaagtatcat ttgggtattt tcggcgaagc ggctaatgaa gtagcaggtg ctgtaagcca
    13441 agagcataaa catcaagctg ttatcggttt cggcggcgag aagaaataga gaaagcacta
    13501 tttctattta gtgccgtttg tccgggcaag atgccgtctg aagtttcaga cggcattttc
    13561 ttcaaaaaac agtccgccaa atcgaatact gccaagcggc aatgcaatac ctcaaaacag
    13621 caataatttc ttttaaaaca gttgattctt actgaacata aatggccggg caatacgaaa
    13681 agaaaaatgg cgttgctggg cattttattt gaatttgtta tgaaattgat gaaatctaaa
    13741 aatatctttt ttatgggata cctaatcgct tcggcgacgt ttgccgagga tatcggcgtg
    13801 cctgtcgaac cgattaacgt cggtagtcgg gctgcgatgc cgtctgaagg ggaaagcctc
    13861 gccctcctgc cgtttgcaga ggatgtgccg ccggttcgcg atgcaatgcc gtctgaagtt
    13921 cctaaaagcg cggcaggcgg cgatgttcga ggcggccgat ctataccgtg agttgttgtc
    13981 ggaaaggtcc gaccttgttt atccccgttt tgatttgggc gtgatgctgt ttgaagacaa
    14041 acaatatcgt gaggcattgg ttcagttgca ccgggccgag gaggcgttgc cgcccgatat
    14101 gcggcagctt gcacgggaat atatccgtca ggcggaagcg gtgcaggcgt ggcatccttc
    14161 gttcaatatg aattacgaac aaacagataa tgtaaacaat gcttcccctt cacgggacat
    14221 cgtcctcaac ggccggaaat ggataaaatc ggaagacagc ctgcccaaac gcgccaacgg
    14281 gataaggtac gaactgggcg ttgatcggat gtttaacatt gcaggcaatc actttgcccg
    14341 cttaggcatc agcggttccg gtgtgcatta ttggaatgcc cgggatttta gcgaacaggc
    14401 gtttcatgcc gaagtaggct accgttatcg gaatagccgt ctagagtggg gattccggcc
    14461 ctttgtcaaa caaaaccggc tgggcaataa ccgttatacg gcaaatacgg gtattgtgtt
    14521 ggattacagc cgccgtctga atgaaaaatg gcgcagcacc caatctttcc agtatggccg
    14581 caaacaatac catgacgaat atttggcaaa acgttacaac agtaaaacga tttcggtgag
    14641 cggtacgttc agctattacg ctatgccggc ttggcagttg tacgggggta ttagcggtat
    14701 gtttgataat actgtggaga aagagcaggc ttcacggcgt tatggtgtaa gtttgggtac
    14761 ggtgaaaata ttagacggcg gtcttggact gaagttgggt gcgggttaca cgaaacggat
    14821 atttaaagcc cctgcaacat taatttacaa ttttactcgg cgtgatgatt aacatcggat
    14881 gagcgcggct ttatggcata aaaaactgtc gtggaaagga tttacacccc aaataaattt
    14941 ccgttacaac aagaacaaca gcaatatgcc cgccttttat tcgcacagcg gcaagggatg
    15001 gtttgtcagt atggaaaaaa cgtattgaca gtattttctc cagccgtccg actgattgtg
    15061 agggatgtcg gtaaatattt atcggcaaac aagaaaatca tcttttttct tgtcgttatg
    15121 cttgactgtc tgcttgcaat aaaaatataa ttccaccctt gccgacatgg tgtcggcaag
    15181 tatttaactc aacaggacga gaaaatatgc caactatcaa ccaattggta cgcaaaggcc
    15241 gtcaaaagcc cgtgtacgta aacaaagtgc ccgcactgga agcctgcccg caaaaacgcg
    15301 gcgtgtgcac ccgtgtatac acgactaccc ctaaaaaacc taactctgca ttgcgtaaag
    15361 tatgtaaagt ccgcctgacc aacggttttg aagtcatttc atatatcggc ggtgaaggcc
    15421 acaacctgca agagcacagc gtcgtactga ttcgcggcgg ccgtgtaaaa gacttgccgg
    15481 gtgtacgtta ccacactgta cgcggttctt tggatactgc aggtgttaaa gaccgcaaac
    15541 aagcccgttc taaatacggt gctaagcgtc ctaaataatt accgggactc aaataggcac
    15601 gtcggccgcc taagctgaac aacggccgag taagtgaata ctcaattggg tattcatggg
    15661 aatagacccg actgaataga ttaaaggaaa ttaaaatgcc aagacgtaga gaagtcccca
    15721 agcgcgacgt actgccagat cctaaattcg gtagcgtcga gttgaccaaa ttcatgaacg
    15781 tattgatgat tgacggtaaa aaatccgttg ccgagcgtat cgtttacggt gcgttggaac
    15841 agattgagaa aaaaaccggc aaagcagcaa tcgaagtatt taacgaagcc attgcaaact
    15901 ccaaacctat cgtggaagtg aaaagccgcc gtgtaggtgg tgcaaactac caagttcctg
    15961 ttgaagttcg tccttcacgc cgtctggctt tggcaatgcg ttgggttcgc gacgcggccc
    16021 gcaaacgtgg tgagaaatcc atggatctgc gtttggcagg cgagttgatt gatgcgtccg
    16081 aaggccgtgg cggtgcgttg aaaaaacgtg aagaagtaca ccgtatggct gaagccaaca
    16141 aagcattctc tcacttccgt ttctaatttt gaaaggctaa taaaatggct cgtaagaccc
    16201 cgatcagcct gtaccgcaac atcggtattt ccgcccatat cgatgcgggt aaaaccacga
    16261 cgacagaacg tattttgttc tataccggtt tgacccacaa gctgggcgaa gtgcatgacg
    16321 gtgcggctac taccgactac atggaacaag agcaagagcg cggtattacc attacctccg
    16381 ctgccgttac ttcctactgg tccggtatgg cgaaacaatt ccccgagcac cgcttcaaca
    16441 tcatcgacac cccggggcac gttgacttta ccgtagaggt agagcgttct atgcgtgtat
    16501 tggacggcgc ggtaatggtt tactgtgcgg tgggcggtgt tcaaccgcaa tctgaaaccg
    16561 tatggcggca agccaacaaa taccaagttc cgcgcttggc gtttgtcaat aaaatggacc
    16621 gccaaggtgc caacttcttc cgcgttgtcg agcaaatgaa aacccgtttg cgcgcaaacc
    16681 ccgtacctat cgtcattccg gtaggcgcgg aagacagttt caccggtgtt gtcgatttgc
    16741 tgaaaatgaa atctatcatc tggaatgaag ccgataaagg tacaaccttt acctatggcg
    16801 atattcctgc cgaattggtc gaaactgccg aagaatggcg tcaaaatatg attgaagccg
    16861 cagccgaagc cagcgaagaa ctgatggaca aatacttggg cggtgaagat ctggccgaag
    16921 aagaaatcgt aggcgcgttg cgtcaacgta ctttggcagg cgaaattcag cctatgctgt
    16981 gcggttctgc atttaaaaac aaaggtgttc aacgtatgtt ggacgcagtt gtagaattgc
    17041 tgccagctcc taccgatatt cctccggttc aaggtgttaa tcctaacact gaagaagccg
    17101 acagccgtca agccagcgat gaagagaaat tctctgcatt ggcattcaaa atgttgaacg
    17161 acaaatacgt cggtcagctg acctttatcc gcgtttactc aggcgtagta aaatccggcg
    17221 ataccgtact gaattctgta aaaggcactc gcgaacgtat cggtcgtttg gtgcaaatga
    17281 ctgccgcaga ccgtactgaa atcgaagaag tacgcgctgg cgacatcgca gccgctatcg
    17341 gtctgaaaga cgttactacc ggtgaaacct tgtgtgcgga aagcgcgccg attatcttgg
    17401 aacgtatgga attccccgag ccggtaatcc atattgccgt tgagccgaaa accaaagccg
    17461 accaagagaa aatgggtatc gccctgaacc gcttggctaa agaagaccct tctttccgcg
    17521 ttcgtacaga cgaagaatcc ggtcaaacca ttatttccgg tatgggtgag ctgcacttgg
    17581 aaattattgt tgaccgtatg aaacgcgaat tcggtgtgga agcaaatatc ggtgcacctc
    17641 aagtggctta ccgtgaaact atccgcaaag ccgttaaagc tgaatacaaa catgcaaaac
    17701 aatccggtgg taaaggtcaa tacggtcacg ttgtgattga aatggaacct atggaaccgg
    17761 gtggtgaagg ttacgagttt atcgatgaaa ttaaaggtgg tgtgattcct cgcgaattta
    17821 ttccgtctgt cgataaaggt atccgcgata cgttgcctaa cggtatcgtt gccggctatc
    17881 ctgtagttga cgtacgtatc cgtctggtat tcggttctta ccatgatgtc gactcttccc
    17941 aattggcatt tgaattggct gcttctcaag cgtttaaaga aggtatgcgt caagcatctc
    18001 ctgccctgct tgagccgatt atggcagttg aagtggaaac tccggaagaa tacatgggcg
    18061 acgtaatggg cgacttgaac cgccgtcgcg gtgttgtatt gggtatggat gatgacggta
    18121 tcggcggtaa aaaagtccgt gccgaagtac ctctggcaga aatgttcggt tactcgaccg
    18181 acctgcgttc tgcaacccaa ggccgcgcta cttactctat ggagttcaag aaatattctg
    18241 aagctcctgc ccacatagct gctgctgtaa ctgaagcccg taaaggctaa tcaggcaaat
    18301 aggccgtctg aaaggctgaa atgatttttc agatggcatt gttctttaat cgatctttaa
    18361 tgtaaaggaa ttagctcatg gctaaggaaa aattcgaacg tagcaaaccg cacgtaaacg
    18421 ttggcaccat cggtcacgtt gaccatggta aaaccaccct gactgc
//
LOCUS       DAXFZR010000046        18107 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000046 DAXFZR010000000
VERSION     DAXFZR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.046"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1257
                     /locus_tag="ACFJAS_001316"
     CDS             <1..1257
                     /locus_tag="ACFJAS_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFC3982556.1"
                     /translation="PKKYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVASA
                     EGSVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVVVGSEAKVNAERGIAIGKEAKA
                     GGKTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAAGD
                     FSTAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGNHS
                     VSLGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGVND
                     FDAVNVRQLKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVGFG
                     TYKGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSDKV
                     LMEQLMQDNRQLKARLEKLEARLNAM"
     gene            complement(1396..1911)
                     /gene="coaD"
                     /locus_tag="ACFJAS_001317"
     CDS             complement(1396..1911)
                     /gene="coaD"
                     /locus_tag="ACFJAS_001317"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC3982557.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2041..2724)
                     /locus_tag="ACFJAS_001318"
     CDS             complement(2041..2724)
                     /locus_tag="ACFJAS_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFC3982558.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2881..3147)
                     /locus_tag="ACFJAS_001319"
     CDS             complement(2881..3147)
                     /locus_tag="ACFJAS_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC3982559.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3221..3691)
                     /gene="rlmH"
                     /locus_tag="ACFJAS_001320"
     CDS             complement(3221..3691)
                     /gene="rlmH"
                     /locus_tag="ACFJAS_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC3982560.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYALKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3744..4130)
                     /gene="rsfS"
                     /locus_tag="ACFJAS_001321"
     CDS             complement(3744..4130)
                     /gene="rsfS"
                     /locus_tag="ACFJAS_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC3982561.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4189..4794)
                     /gene="nadD"
                     /locus_tag="ACFJAS_001322"
     CDS             complement(4189..4794)
                     /gene="nadD"
                     /locus_tag="ACFJAS_001322"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC3982562.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPVAARYIRKHGLYEK"
     gene            complement(4791..5390)
                     /locus_tag="ACFJAS_001323"
     CDS             complement(4791..5390)
                     /locus_tag="ACFJAS_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC3982563.1"
                     /translation="MSELSEILSYNQKFVESGGYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5419..6960)
                     /locus_tag="ACFJAS_001324"
     CDS             complement(5419..6960)
                     /locus_tag="ACFJAS_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3982564.1"
                     /translation="MDGRCWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLVWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLSYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7319..8704
                     /locus_tag="ACFJAS_001325"
     CDS             7319..8704
                     /locus_tag="ACFJAS_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC3982565.1"
                     /translation="MDGRTQTLSAQTLLGISAAAIILILILIVKFRIRALLTLVIASL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFAPGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASVGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRAIHVPVP
                     ELLSGGTRDSDPPKEPAKAGTVVAVMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KMIGSTPVALLISVLAALLVLGRKRGESGSTLEKTVDGALAPACSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            8724..9242
                     /locus_tag="ACFJAS_001326"
     CDS             8724..9242
                     /locus_tag="ACFJAS_001326"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC3982566.1"
                     /translation="MTTHFVVMGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9428..10345)
                     /gene="thrB"
                     /locus_tag="ACFJAS_001327"
     CDS             complement(9428..10345)
                     /gene="thrB"
                     /locus_tag="ACFJAS_001327"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC3982567.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFVGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10382..11110)
                     /gene="ubiG"
                     /locus_tag="ACFJAS_001328"
     CDS             complement(10382..11110)
                     /gene="ubiG"
                     /locus_tag="ACFJAS_001328"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC3982568.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECVRVEDLAGAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVRPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFISPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11258..12499
                     /locus_tag="ACFJAS_001329"
     CDS             11258..12499
                     /locus_tag="ACFJAS_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC3982569.1"
                     /translation="MSAKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLIVLL
                     YTWFSMLSGGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVSTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            12651..13349
                     /locus_tag="ACFJAS_001330"
     CDS             12651..13349
                     /locus_tag="ACFJAS_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC3982570.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIVAHSHMMRRELVGLYGVPPERIQ
                     VAPPPQIRNASFHNLEKLPPCAPNTVLPTMKPFSYSHRPATRAKAWNCSPTFSNKPNC
                     PSSSPLPVHRFPAR"
     gene            13247..13705
                     /locus_tag="ACFJAS_001331"
                     /pseudo
     CDS             13247..13705
                     /locus_tag="ACFJAS_001331"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(13772..15424)
                     /locus_tag="ACFJAS_001332"
     CDS             complement(13772..15424)
                     /locus_tag="ACFJAS_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFC3982571.1"
                     /translation="MVAYAFLFLFVTAAVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFAKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            15567..16130
                     /gene="gmhB"
                     /locus_tag="ACFJAS_001333"
     CDS             15567..16130
                     /gene="gmhB"
                     /locus_tag="ACFJAS_001333"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC3982572.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            16160..16903
                     /locus_tag="ACFJAS_001334"
     CDS             16160..16903
                     /locus_tag="ACFJAS_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC3982573.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            16900..17588
                     /locus_tag="ACFJAS_001335"
                     /pseudo
     CDS             16900..17588
                     /locus_tag="ACFJAS_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
     gene            complement(17652..17843)
                     /locus_tag="ACFJAS_001336"
     CDS             complement(17652..17843)
                     /locus_tag="ACFJAS_001336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982574.1"
                     /translation="MPSENLSDGIFAGKSVEDFAPAQAFHTARRIGRVSEGRCEMKRC
                     GRRGCRHPAPNRRQSVIAV"
BASE COUNT         4308 a         5470 c         4605 g         3724 t
ORIGIN      
        1 cccaagaaat atgcagtagc attaggatct agttctactg ccagtggaga gtattcctat
       61 gcgtcgggct ataattctgt tgccagtgga aataaatcct atgctgcagg ctatgcttct
      121 gttgccagtg cagaaggcag tgttgtaatt ggcgattcaa ggcaagtaaa accggaagca
      181 gatcaagggg ttgcagtagg ttctaaagca acggtaaaga acaaagctaa acaaagggtt
      241 gtggtaggtt ctgaggcaaa ggtaaatgca gaacgtggta ttgcgattgg taaagaagcc
      301 aaagcagggg gcaaaacaac gaacacactg ttagatggac cggcatatta tgcggacgct
      361 attgccgtcg ggtatcaagc agaggctggc aaaggtggcg ccatcgcttt gggaaaacaa
      421 gcaaaagcaa cgaagcaaaa tggaatggca cttggcgtag aatcagaagc tgccggtgat
      481 tttagtaccg ccgtgggtaa tgaaagcaag gcaaaggggc aaggcggcgt aggtctcggt
      541 aaccaatcta aggcagaggc agattttgcg gtggctgtgg ggaataaggc tgaagcgact
      601 aaggaaaact cacttgtcat tggccgatat gcaagggcaa acggaaatca ttctgtgagt
      661 ttaggatctc gtagtgaaat aaaagatggt gtgagcaatt ctgtggcacc gggttatggt
      721 tcagtagcta gtgagaataa tgtcgtctct gttgcctata aagaaacacc acaatctaca
      781 gaactttcct atagaaaaat tgtaggtgtt gatgatggtg tcaatgattt tgatgccgtc
      841 aatgtacgcc agttgaaagc gatgcaagga caaaatatgg ctgagctatt ttcagtacgc
      901 agcgaagtgc gtggtgtggc agcgagtagt gcagccctat cggctctgac tccattatcc
      961 tatgatgcaa acaatcctac gcagtttatg gtcggtttcg gcacatacaa aggacgtcaa
     1021 gctatggcat taggattgtc ccatttcgtc aatgaccgct ttatggtaaa agtaggcgga
     1081 accttaggcg gcaataagac gggacatatg atgaatgtag gcttgacttg gaaatttgga
     1141 acgagccaag gtgttccggc tacaagtgat aaggtgctta tggaacagtt gatgcaagat
     1201 aatcgtcaat taaaggcacg attggagaaa ttagaagcac gtctgaatgc tatgtagcct
     1261 tgtggcgata acggtttatt gagtatatcg ccaatgataa agtgcataat tttcaaaccg
     1321 ttgataatat acaataaatt gtaaccaata cctcaaacgg gtttgaaccc gaatatgcct
     1381 gacaggaact ttctgtcagg cattgttgtt gtgatgttct gcaatcattt tttggtacac
     1441 ggccggcggc acatatcttt tgacggtttc catccagccc tcgggcccga ccagtccttt
     1501 gatcatagtg gacgacactt cggcgatttc gcgcggcggc atgaggaata cggtagatat
     1561 ttcgggtgcg aggtcgctgt tgatatggcg catggagcgt tcgtattcat aatcggaggt
     1621 ggaacggatg ccgcgcacga tgaatcccgc atctacctca cgggcgtaat gcaccaaaaa
     1681 ccggttttga aacacttcaa ttctgacgtt tgggaagttg tcggtaatgg cgcacaacat
     1741 atcctgcctt tcagcggcgg tataggtgtt gcgtttgtcg ggattgatgc cgatggcgac
     1801 gatgagttcg tcaaacatag attgcgcctg ccgtatcatc cacagatgcc ccaatgtggg
     1861 cggatcgaaa ctgccggcat aaacggcgcg gcgcggtgta ttcggtaaca tttgattcct
     1921 cccggcttca tagccggctg tgtttttatg tgtttgcatc cgtattgtat gcccaaaaca
     1981 aaatgccgtc tgaaaataag tgctttcaga cggcatggtt tgcatggtgt ttcgcctaca
     2041 tcagtcgtcg ccgtttaagg acagcaggat gttgagcagg ctgctgaaga tgttgtaaag
     2101 cgagataaac agtgtcagtg ccgcgctgat gtaactgtct tcgccgccgt cgatgacggt
     2161 gcgtacctgc cacataatca ttaaggaact gaacaagaca aaaccggcgg aaatggtcag
     2221 ggcaagtgcg ggaataccca aaaacagatt ggcaaccacg gcgaccatca gaatgaccgc
     2281 acctacggtc aggaagcgtc cgagcgcgtt catatcgagc cgggttcggc gtgccaatgc
     2341 ggacatcgtt aaaaagacgg cggcggtcat cgcggcggca atgccgacga ttttcgcacc
     2401 gtcggcaata tggagtgcgt attgcagcac ggggccgatc aatacgccca taccgaatgt
     2461 gaataccatc agcagggtaa cgccggtatt gctgtaacgg tttttttcga tgaagtggat
     2521 cataccgtag aaaaacgcca atacgacggc aaatcctatc cagcgcgaac cgaaggcggc
     2581 gtaaaaattg aaaccggcat tggcggcaag tgccgcgcct gcggctgccg gaatgaatga
     2641 aaatccgagc aggcggtagg ttttctgcag gacggtgttt ttagaaaccg tatgcgcggt
     2701 gtagtcgtaa acgtcgtgtt gcatatcatc tgctcctgaa agcgcggttg gaaataacgg
     2761 gggattttaa cattgcccaa tgtcaaaatt tgtccggttg cgtgaagata aagttgtccg
     2821 gcgtatttta aaggccgtct gaagcagttt cggacagcct gtgttcaaaa cggaaaaccg
     2881 ttattgcgga acgtatccct gcacggcatc cgcgccgtcg ccgaagaaat actgctccat
     2941 ctgctgagcc aggtattcgc gcgcgcgcgg atcggcaagg cttaagcggt tttcgttaat
     3001 cagcatggtt tggtggcgcg tccacgccgc ccatgcttct tgcgatacgt tttcaaaaat
     3061 gcgtttgccc aattcgttgg gcagcggcgg aaatttcatg ccttcggctt ctttgttgag
     3121 cttgacgcag aataccattc gtgccatagc ggattccttt gctgtgttca gaaataacgg
     3181 ggcgatttta accgattagg gggtacagac aaaagccttt ttattcccga tgataaggat
     3241 ggttgtgcag gatggaaacg gcccggtaga gctgctcggt cagaaggacg cgcaccatgc
     3301 cgtgcggcag ggtgaggctg gacaggcgca tcatcatgcg ggcttgttgt ttgaggcggt
     3361 cggtcatgcc gtccgcgccg ccgatgacga agcagacgtg ttcgccgttt tgccgccagc
     3421 ttttgaggtg ttccgccagc tcgacggagg tcggtgcttt gccgcgttcg tcaagaacga
     3481 cgaggaacgc gccttgcgga atggcttcaa ggatgcgttt ttcttccgcc gccatacctt
     3541 ggacggcatt cacgcccgcg ccgcgttttt cgggtttgat ttctttgagt gcgtaggcgg
     3601 cgtcgcgtcc gaagcgtttg gcgtattcgg cgacggcctc atcaacccaa cgcggcattt
     3661 tggtgccgac tgccaagacg gtaatgttca atgctttccc ccttacagga aatgccgtat
     3721 gaatattcag acggcatcgg gaatcagtct gcagcgtgcc acggcttctg cattccggcg
     3781 tggaaactcg gtttctcgcc gccccagatg gtgtcaatgt cgtagaagtc gcgcacggca
     3841 gggagcatga cgtggacgac gaggtctcct gcatcaacca acgtccattc gccgctgtct
     3901 ccttcggtac tgaggatttc aaaaccggct tctttcaaat cgacggcaac gttgttggcc
     3961 agtgctttga cttggcgcgt actgtcgccg ctggcgataa tcattctggc aaacagcgaa
     4021 gttttgtctt gcgtttcgag aacggaaatg tctttggctt tgatgtcttc gagggcattg
     4081 acggcgaccc cgaccatttt ttgcaggtct tgcagttctt gttcgttcat tgttttccta
     4141 acgggatgtt ttcagacggc attatagccg ttccttactg atttgacttt atttttcata
     4201 caaaccgtgt tttcggatat aacgggcggc gacaggcggg atgccgtctg aaacaccggc
     4261 ggcactcagg ttgcggcgga tttccgttga cgacacatta tgcatcgggg ctgacaagat
     4321 gcggacgctg ccgtcctgaa gggcattgcc cagccacgcg tgcagttcgc gcggggtttg
     4381 gtgcaggctg tcgccctgcc gcatggcgac ggcgatattg gtttcgcgca cgagcaactg
     4441 ccattttttc caagtatgca gcttcagcag gctgtcgctg cccatcagcc accaaagttg
     4501 cgcggacggg aactgtcggc ggaagatttg gacggtatca aaagtatagg ttgcactttc
     4561 tcggacgatg tcgcagtcgc tgacggcaaa acgcgcgtct tctgccgtcg ccagttcgac
     4621 catggcaagg cggtcggcgg cggaagcgga ggcggcgtct ttgtgatacg ggccgcctgc
     4681 cggcaggaaa acaaccgcgt ccaacccgat ttcgtcggca aaggcacggg cgatatgaaa
     4741 atgcccgttg tgtatcgggt cgaaagtgcc gccgaacagt ccgattttct tcatgatgtt
     4801 tccattcctt ccgataagtc catgccgtct gaaacactgc cgtccacaac cagcgtcagt
     4861 ttgccggtaa cgggatggat gaccagtccg tgaacggcga tatggcgcgg catcagcgga
     4921 tggttgcgga taaggtccac cgtgtggcgc acgctgtctt cgacgttgtc gaaaccggtc
     4981 agccagccgt cgaggtcgac acctgcataa cgcagggttt cgatacggtc ttcgggaatc
     5041 cggctttccc ggacgcgccc gaggaattct tcggcattca gcccctgcat accgcaatcg
     5101 tgatgggcga tgaccataat ctctctgacc ttcaattcaa acacggcaac caaaaggctc
     5161 cgcatcaccg aaccccacgg gtgcgtaacc agcgcgccgg catttttaat cagcttggca
     5221 tcgccgtttt tcaaacccaa cgcgtcgggc agcagcccga taatccgcgc atccatacag
     5281 gacaaaaccg ccagcccgcg ttcggggtat ttgtcggtaa agtatttttc atatcctccc
     5341 gactcgacaa acttctgatt ataggaaagg atttcgctca actcgctcat tttgccgtcc
     5401 tctgaaaaag ggttcgcatt ataacgtttc cgtccgtttt ccgccttcgc cgttgtccaa
     5461 cagcaggaaa atgcacaccg caaatgccga caacagcaat gtcaacacca ttgcccgcgc
     5521 ataattgtcc tcacccgcac gccccaaata ggcataaatc aaagtcgtca acgtctgcca
     5581 ttccggacgc gacaggaaca aggttgccgc aaattcgccc acacacgtcg ccgccgccaa
     5641 agtcagaccg cgccgcaacg ccggtttcaa gagggggaac gtgatacggc atgccgtctg
     5701 aaagccgttt gcgcccaaac ctgccgccgc cctgccgtaa tccggcggca gtgcatccca
     5761 ggccgataaa acatcttttg ccacaaacgg atacgacagc agcgcataca tcgccagcag
     5821 cagcggtaac gaagccgtcc accccggata aagcagcagc acgcccgccg aaacacaaac
     5881 cggcgacacc ataaacggta aaaacaccag tccgcgcatc cacaccagcc gccgcgccgc
     5941 cgccgcatac accacaccca aaaccgccgc cgcaaacacc gccgccgccg aaaagcgcaa
     6001 agtattccac actgcctgcc acgtttcact ttccattaac acacgccgcg attcgccggc
     6061 cgaccacgct ttcacaacaa ttgccgacaa aggaaacagg cagcacacgg acaacaccgc
     6121 caccgaaaat gccagcaata catattcccc caccgattgc ggcggcgacg gcatcacggg
     6181 ggaaaccgcc ttatccgaaa ccgcgcgcct gccgaaccac gcatacagca accctgccgc
     6241 cgccgttacc cccaacacca gccacaccag cgccgaagcc cccgccatat cgagttcgaa
     6301 cataaccaac tggtaaattt ccacttcgac cgtggcataa cggctgccgc ccaacagcaa
     6361 tgccagcccg aaccccgaaa aacaatacag gaagacaagg cacacgccgc cggcaagcca
     6421 cgggcgcaaa acgggcattt caatgtccca aaaccgccgc cacgcccccg cgcccaacgt
     6481 ccgtgccgtc tgaagccgtg ccgcaggcac ttgagcaaac ccctgatacg ccgccctgac
     6541 caacacgggc aggttaaaaa acacattgcc gtacaacaac agatacggcg tatcctgccg
     6601 gccgcgccac aacagcccgt ccgccccgaa cagagccagc acgcccacgc ccgccaccag
     6661 cgtgggcatc acaaacggca gcatcagcag gcgcagcacc aaagcccgcc ccgggaacgc
     6721 cagccgcgcc agcacccacg cgacaggcac gcccaaaggc agcaccagca cacaggttgc
     6781 cgccgcctga aacaccgtcc acgccaaacg tttgagcata taggcatccg acagcaccgc
     6841 gcgccacgcc aaaccgtcat acgccgccac cgcccacaaa ggcgcaacga ccattaccgc
     6901 caaaaaagcc gaaggcagca gggaaaaagc accccgtacc gcccaacacc gtccatccat
     6961 cgccttcccc acttgaaaca ctgatgttgc gattgtaccc aaaagcccca cataccgtat
     7021 atttcaatcc gactacgtac cgtatccgcc ttcctcccgc cgtctgaaat atagcggatt
     7081 aacaaaaatc aggacaaggc ggcgggccgc aggcagtaca aatagtacgg aaccggttcc
     7141 ctttgagcta aggcggggca acgccgtacc ggtttttgtt aatccactat aaatcgttca
     7201 aataaacagg aatataactt cagacaaaca acttaccgcc cgatttgtgc tatcgttttc
     7261 gcacaactta aaaaaacctg acaattttgt acttttatta cagagaaagg ctttacaaat
     7321 ggacggccgg acacagacgc tgtccgcgca aaccttgttg ggcatttcgg cggcggcaat
     7381 catcctcatt ctgattttaa tcgtcaaatt ccgcatccgc gcgctgctga cactggtcat
     7441 cgccagcctg ctgacggctt tggcaaccgg tttgcccaca ggcagcatcg tcaacgacgt
     7501 actggtcaaa aacttcggcg gcacgctcgg cggcgtggcg cttctggtcg gtctgggcgc
     7561 aatgctcgga cgtttggtag aaacatccgg cggcgcacag tcgctggcgg acgcgctgat
     7621 ccggatgttc ggcgaaaaac gcgcaccgtt cgcgccgggc gttgcctcgc tgattttcgg
     7681 cttcccgatt ttcttcgatg ccggactaat cgtcatgctg cccatcgtat tcgccaccgc
     7741 acggcgcatg aaacaggacg tactgccctt cgcgcttgcc tccgtcggcg cattttccgt
     7801 catgcacgtc ttcctgccgc cccatccggg cccgattgcc gcttccgaat tttacggcgc
     7861 gaacatcggc caggttttga ttttgggtct gccgaccgcc ttcatcacat ggtatttcag
     7921 cggctatatg ctcggcaaag tgttggggcg cgccatccat gttcccgttc ccgaactgct
     7981 cagcggcggc acgcgagaca gcgacccgcc gaaagaacct gccaaagcag gaacggtcgt
     8041 cgccgtcatg ctgattccca tgctgctgat tttcctgaat accggcgtat cggccctcat
     8101 cagcgaaaaa ctcgtgagcg cggacgaaac ctgggttcag acggcaaaaa tgatcggttc
     8161 gacacctgtc gcccttctga tttccgtatt ggccgcactg ttggtcttgg gacgcaaacg
     8221 cggcgaaagc ggcagcacgt tggaaaaaac cgtggacggc gcactcgccc ccgcctgttc
     8281 cgtgattctg attaccggcg cgggcggtat gttcggcggc gttttgcgcg cttccggcat
     8341 cggcaaggca ctcgccgaca gcatggcgga tttgggcatt cccgtccttt tgggctgctt
     8401 ccttgtcgcc ttggcactgc gtatcgcgca aggttcggca accgtcgccc tgaccacagc
     8461 cgccgcgctg atggctcctg ccgttgccgc cgccggcttt accgactggc agctcgcctg
     8521 tatcgtattg gcaacggcgg caggttcggt cggttgcagc cacttcaacg actccggctt
     8581 ctggctggtc ggccgcctct tggatatgga cgtaccgacc acgctgaaaa cctggacggt
     8641 caaccaaacc ctcatcgcac tcatcggctt tgccttgtcc gcactgctgt ttgccatcgt
     8701 ctgacagacg gaaaggatag taaatgacta cgcattttgt cgttatgggc gtatgcggct
     8761 gcggcaagac caccgccgcg ccgtccctgc agaaacacct cggtcaatgt ccctatgccg
     8821 aaggcgacga gttccacacc caagccaacc gcgacaagat gggcgcgggt attccgctga
     8881 ccgatgaaga ccgctatccg tggttgggca atctgcgcga ctggatgacg caacaggcgc
     8941 aaaacggcgc ggaccacacc atcgtaacct gttccgccct caaacgcggc taccgcgaca
     9001 ttctgcgcgg agccgaaggc aaagccgcct tcatccacct cagtccgccg caagacatca
     9061 acctcgaacg catgatgtcg cgcaaagggc actacatgaa agcagggatg ctcgattcgc
     9121 aactggaaat cctcgaggaa ctgggcgaag gcgaatacgg ggtcaaaatc gccaaccccg
     9181 gcacgcccga agcggtcgaa gccgatattc tgaactgggt tgcctcggaa aacctgcttt
     9241 gaagcaatat gccgtctgaa gcccggcaca ggatgggttt cagacggcat aaacatcggg
     9301 aacagaatga attacattga tttaaaatgg aaaatacccg gctatcgtct cattttcgtt
     9361 ttaatcagcc ataaaaatgc cgtccgaaac cctttcagac ggcatttctg tcaaacgccg
     9421 gacgcactca atccaaactc aacagcaggt tgcggaacgc gttcgggtct ttgataaacg
     9481 tcatctcgcc cgcctgcgga aaatgaaaat ccaacaggcg cgacacccaa aaacggatgc
     9541 agccggcgcg ttgggcggtc gggaaatacg ccttttcccc ggcactcaag gggcgcacgc
     9601 cctcataacc gccgacaaac gcctttttca acgcctcatc caacttattg tccgccgtcc
     9661 ttgcccaatc gttgaccgca atcgccaagt catacataaa attgccccgg caggcataat
     9721 agaaatcgat gaagcccgat acctgaccgc cgtcaagcaa cacattgtct ttaaacaggt
     9781 cggcgtggat gatgcccgaa ggcagatggt tgccgagatt gtccttcagc gcatcgattt
     9841 cggaacacag cagcgcggca tcgtcttgcg acaggacggg cagcagccgg gcgcacgctt
     9901 ccgtccacca cgcatcgtaa cgcgggtttt ccatttccaa agggaaatcg gcggcggcaa
     9961 ggtgcatttt cgccaacatc gcaccggtat ggaaacactg ctcagccgtc ggcagcgcgg
    10021 tatccgaacc tttcaggcag gcaaccaggc aggccggctt gcccgccaaa acggaatcga
    10081 gccggccgtc tttgcgcgca accggcgcgg caaccgccac gcccttcata ctcaaatgcc
    10141 ggttaagctc cagaaaaaac ggcagctctt cctgtttcaa cacttcaaac acggtcagca
    10201 cataacgtcc cgaagtcgtc gtcagaaaat aattgctgtt ggtaatcccc tgcgcaatgc
    10261 cctgcaggga aacaaattcc cccaaatcgt aaccgctcag gaagccgcgc atttcatcat
    10321 cggaaacact ggtatagaca gacatatcaa ctcacttgct caaaaacggg caaacccgcc
    10381 gtcagaacgc cggacggcag gcaaacatat aattcacatc ggtcgaatcg cacagggcat
    10441 aagtttgcga caacacatgg taagtcatac ccttcgtatc cgccacatcc aagcccgcct
    10501 gacggcacat ccgcgccagc tcggcaggcg agatgaattt tttccagtcg tgcgtgcctt
    10561 tggggacaaa cttcaacaga tattccgccg ccacaatcag atgcaggtac gatttcgggt
    10621 ttttattgat ggtggaaaaa aacaccatgc cgtccggcct gaccagcttg gcacaggcac
    10681 gcacgatggc ggcgggatcg gggacgtgtt ccatcatttc catgcacgtt accacatcga
    10741 acgagtgcgg ttccgccccg gcaaggtctt ccacgcggac acattcgtat tcgatatcgg
    10801 cgacattgtt caaagccgcg tgcaggcggg cggtttccaa cgactgctcc gccatgtcga
    10861 tacctttgac aaacgccgcg ccgcgccgcg ccatgctttc cgcgaggatg ccgccgccac
    10921 agcctacatc taaaacccgt ttgccgcgca aatccgcgtg tccgtcgata taatccagcc
    10981 gcagcggatt gatgtcgtgc aaggttttga actcgcccga cttgtcccac catttgtcgg
    11041 caatccggct gaacttggcg atttccccct catcgacatt atattttttg tcggacattt
    11101 tccctcccat ctgacgaacc gccccactca aaaacccaag attatactat gggacgcaat
    11161 gccgtctgaa agccttttcg ggcggcgcgg cgcaaatacc ttacaaaccc ttacacttta
    11221 cggcataatg caggctgttt ttcccgagga ggcttatatg tccgccaaaa ctccgtcgct
    11281 cttcggcggc gcgatgatta tcgccggcac ggtcatcggc gcaggtatgc tcgccaaccc
    11341 caccgccacc tccggcgtat ggtttacagg ctcgctgatt gtgttgcttt acacttggtt
    11401 ttccatgctc tccggcggcc tgatgatttt ggaagtcaac acccattatc cgcacggcgc
    11461 aagtttcgac accatggtca aagacctgct cggacgcggc tggaacatca tcaacggcat
    11521 cgccgtcgcc ttcgttttat acctgctcac ctacgcctac atcttcgtcg gcggcgacct
    11581 gaccgccaaa ggcatcggca gcgcagtagg cggcaaaatt tcgctcaccg tcggacaact
    11641 cgtcttcttc ggcatcctcg ccttttgcgt atgggcatcc gcacgcttgg tcgaccgctt
    11701 taccggcgtc ctcatcggcg gcatggtatc aacctttatt tgggcaaccg gcggcctggt
    11761 tgccgatgcc aaaccgtccg tcctcttcga cacccaagcc cccgccggca ccggctactg
    11821 gatttacgcc gccaccgccc tgcccgtctg cctcgcttcc ttcggcttcc acggcaacgt
    11881 ttccagcctg ctcaaatact ttaaaggcga cgcgcccaaa gtggcgaaat ccatctgggc
    11941 aggtacgttg gttgccttgg taatttacgt cctctggcaa accgccatcc aaggcaacct
    12001 gccgcgcaac gagttcgccc ccgtgattgc cgccgaaggg caagtctccg tcctgattga
    12061 aaccctgtcc aaattcgccc aaaccggcaa tatggataaa atattgtccc tattttccta
    12121 catggcaatc gccacctcgt ttttaggcgt aaccttaggc ctgttcgact acatcgccga
    12181 catcttcaaa tggaacgaca gcatgtccgg ccgcaccaaa accgccgcgc tgaccttcct
    12241 gccgcccctg atttcctgcc tgctcttccc caccggcttc gttaccgcca tcggctgcgt
    12301 cggcctggcg gcaaccgtct ggacaggcat catccccgcc atgctgctct accgttcgcg
    12361 caaaaaattc ggcgcaggca aaacctataa agtttacggc ggcttgtggc tgatggtttg
    12421 ggtcttcctt ttcggcatcg ccaacatcgc cgcacaggta ttgagccaaa tggaactcgt
    12481 ccccgtattt aaaggataaa ggcaaaatgc cgtttgaaac ccgccggcgg cttcaggcgg
    12541 cattgccgca acaaacggca accgtattcc ggcacacagc gcattaccct accccctcac
    12601 gcacaaatcc cgccccgaca ggcgcgggac gcaaccataa aggaacaatg atgaagctca
    12661 aaatagacat tgcaaccaac aacttcaaac acggcggcgg cacggaacgc tacacattgg
    12721 atttggtaaa gggtctgaac agacaaaaca tcacaccggc cgtttatgcg acgaaatttg
    12781 atcacggcat tcccgaatac gccatgatcg aaccccatct tgtcgatcaa cgccggacgc
    12841 tgaaaaaact acgctcgttc ctcttttcaa gccggctcgc tcaaaccaga aaaaacagtg
    12901 ccgccaaact gattgcctgc caccacgccg attacgccga cctcctcatc tgcggcggca
    12961 cgcacttggg ctacctgcac catatggcgc aaaaaccgaa cctgctcgac cgcctcgcca
    13021 tacgccgcaa ccgcagcaac tacgccaccg ccaaactgat tgtggcgcat tcccatatga
    13081 tgcggcgcga actggtcgga ctatacggcg ttccccccga aagaatccaa gtcgcccccc
    13141 ccccgcagat acggaacgct tctttccaca acctagagaa actgccgccc tgcgcgccaa
    13201 atacggtttt gccgaccatg aaaccgtttt cctattccca tcgaccggcc acacgcgcaa
    13261 aggcctggaa ctgctcgccg actttttcga acaaaccgaa ctgcccgtca agctcgccgt
    13321 tgccggttca ccgcttcccc gcccgatgaa aaacgtcgtc ggactgggct tctgcaccga
    13381 tatgcccgaa ctctaccgcg ccgccgactt taccattatg gcatccctgt acgaaccctt
    13441 cgggctggtc ggtgtcgaat ccgccctatg cggcacacgc gtcgtcctct ccgaaaacat
    13501 ggcatgtaca gaggtcatga acgaagaagc cggattcttt ttctcacgac aaaacccgga
    13561 aaccctcgcg caagccgttg cccaagccgt cagccttaaa aaacagggcg gacaccgcct
    13621 gtccgacccg atgcgggcgc tgaactacaa cccgtccctg tcgcaccaca tcgaccgact
    13681 gaccgacatg ctggcatccg tataacaaaa tgccgtctga acgggttttc agacggcatt
    13741 ttcgcaaccg ttgcagccgt gcggcaaaaa gctaacgggc aattttcaaa acgtcgtaca
    13801 gatagttgcg gtaatttccg accaactccc ggttcgggca gtcttccaac cctccgccgc
    13861 acaaaccgcg catcgccata ttcgcacgca tgaagccgtc tttggcttcc aaaccagccg
    13921 cctccatcag tacgccgcct gcaaaggcaa ggtcgaggaa atcctgccgc tgtacgaatc
    13981 cgccggcaat attgctcctg acggcaaact gcgttacata atccggctga gcgtaatccc
    14041 atttcttcct gacggacacg ccctcaaacg gtacctgatg atcgccgaaa taaccgaaaa
    14101 caaagggttt gccgcgttcg tgcagataac ggtcgaaact ttccaccgct ttgtccaaat
    14161 cggcaatgcg cccgatgtag tcgttaagcg cggatacggt tttcgcattc aaatcgggcg
    14221 catccaaatc aaacacattg tcggtatccg tccgatacgg cccgtgctcc ttcatggtca
    14281 gcacatatac gaacatcggc tgccgcacgt tttccaaatc gggatggcgt ttttcgagaa
    14341 tcatccgcgc atactgcatc atttcctcac tggaaatgtg ccacaggttt ttgcccatcg
    14401 gcgcgggata gcccaaatct tgcggctgga acatcagatt aaagccgaaa tggtcatatg
    14461 ctgccttggc attgtagttg cccttggcaa atggcgagag cgccacgcaa aaataaccgt
    14521 gttcgcgcag gttgcggaca aaaccggtct gcaaatgcgg tacgaccgaa taaaacacgc
    14581 cgcttgccaa cgcgccgaaa tctgtggacg gaacacccgc caaaaacgca aattcggact
    14641 tccacgttgc gccgccgaaa gtatgcacgc gcaaaggtga ggaaaataca gtatcttcct
    14701 gccgtccgaa cattttcaaa tcgggaattt tggcggcggc aaaatcgaaa cagtgcggat
    14761 cgagcgtcga ttccatcagg gtaacgacaa tatcgggctt ttcatccgac atgccgtacg
    14821 gccgggtttc agacggcatc tgcctggcaa acgcttcccc gtcgccctcg aacacgggta
    14881 cttggaaaaa caccgcccga caggacatcg gcaggttcaa aaacacgtcg cgcccgtcgt
    14941 ccggcagcga gtccagccac gtctttaccg cgccggggtg cttggaaaaa tgccgcaccg
    15001 acacgaacgc cgccgcaaac agaaccgcgc ccgcccaacg ccacggcata cccaaagaat
    15061 ccgcaccgct ccagccgaaa accgcatatg ccgccaaagc cagcagcccc gccataccga
    15121 taaccgcttc cttataatga aacagcgttt cccaattccg ccagtcggca atcagcaaaa
    15181 agtcggaaat caacagcggc tgtttgtaat aatggatttt cagcctgtgg aacagtatca
    15241 gcaccacaaa ccagaccgaa gcgaaattca aggcgcgctg ccactgcgcc gtcaacatca
    15301 gcataccgcc cgccaaaaag acaaacagcg ccgacgcgaa aaaatacgtc cagcggtagt
    15361 gcgacctgac aatcagcagc accgccgccg ttacaaacaa aaataggaaa gcataggcaa
    15421 ccatcccgcc ccactcctca aaacggcata cctgtgccgt tgcgaattat tatcgaaagc
    15481 atgcattata atgcagcaca ggcgcatttc caccgatatt tcagtataat gccatccccg
    15541 aacccgccca acccaaagga accgtgatga aactcatcat tctcgaccgc gacggcgtca
    15601 tcaaccggga ccgcgacgac ttcgtcaaat ccgttgacga gtggatacct gtcgaaggca
    15661 gcatggatgc ggtggcattc ctgacgcagg caggctgcac cgttgccgtc gccaccaacc
    15721 aatccggcat cggccgcaaa tattttaccg ttcaagacct caccgaaatg catgccaaaa
    15781 tgcaccgcct catccgtcag gcgggcgggg aaatcgacgg catctggttc tgcccgcata
    15841 ccggcgccga cggctgcaac tgccgcaagc ccaaaccggg catgattgaa gacatcctcg
    15901 aacgcttcaa cgcccaagct tcggaaactt ggctggtcgg cgacagcctg cgcgatttgc
    15961 aggcaatcga tgccgtcggc ggaaaacccg cgctggttct gaccggaaaa ggcaaaaaaa
    16021 cgctctccca acacggacac gaattgcccg gacacacaca ggttttcgat accctgctcg
    16081 atttctcaca atacatcatg caggaaaacg ccgcaccgca agccgactga acataccgca
    16141 tttccgacaa ggcaaaacca tgctcatcat ccgcaacctg atttactggc tgatactctg
    16201 ttccagcctg attttcctct ttccctttat gctgctcgcc tcgcctttcc gggacggggc
    16261 gcacaagatg gcgcgggtct gggtcggcat cctcaactgg tcgctcaaac acatcgtcgg
    16321 gctcaaatac cgcatcatcg gcgcggaaca cattccggac cgcccctccg tcatctgcgc
    16381 caaacaccaa agcggctggg aaacgctcgc gctccaagag atttttccgc cgcaggttta
    16441 cgttgccaag cgcgagttgt tcaaaatccc ctttttcggc tggggcttga aactggtcaa
    16501 aaccataggc atagaccgca acaaccgccg cgaagccaac gaacagctca taaaacaggg
    16561 tttggcgcgc aaaaacgaag gttattggat taccattttc cccgaaggca cgcgccttgc
    16621 gcccggaaaa cgcggcaaat acaaactcgg cggcgcgcgc atggcgaaaa tgtttgagat
    16681 ggacatcgtc cccgtcgccc tcaacagcgg cgaattttgg ccgaaaaatt cctttctgaa
    16741 atatccgggg gaaatcaccg tcatcatctg tccgaccatc ccgcacgcaa gcggcagcga
    16801 agccgaattg atggaaaaat gcgaacatct catcgaaacg cagcagccgc tcatttccgg
    16861 cgcaggcccg tttgccgccg aaatgccgtc tgaaaccgca tgacctccct gattcacacc
    16921 ctttcagacg gcatagaact gaccgtcgaa atcaaccgcc gtgccaagaa aaacctgatt
    16981 atccgcccta tcggcacaca taccgtccgc atcagcgtcc caccctgctt ctccgtctcc
    17041 gccctcaacc gctggctgta tgaaaacgaa gccgtcctgc ggcggacact ggcgaaaaca
    17101 ccgccgcaca atacggcaaa ccgactgccc gaacatatct ggttccacgg caggcagctt
    17161 gccccaccgc ccatcaggac acgcaaatcc tgctgatgcc gcctgaaatc ctcgtccccg
    17221 aaggcgcgcc cgaaaaacag cttgcgctgc tgcgggactt tttggaacgg caggcgcaca
    17281 gttacctgat tccccgtctc gaacgccacg cccgcgccac gcaactgttc cccgcctcct
    17341 cctcgctgac ctccgccaaa accttctggg gcgtgtgccg caaaaccaca ggcatacgcc
    17401 tcaaccggcg gctggtcggc gcacccgaat acgttgccga ctatgtctgc atacacgaac
    17461 tctgccacct cgcccatccc gaccacagcc ccgccttttg ggaactgacc cgccgcttcg
    17521 ccccctacac gcccgaagca aaacagtgga tcaaaatcca cggcagggag cttttcgcct
    17581 tgggctgacg cagccccggg ccgacgcttt cagacggcat ccgtgccgga acaggcacgc
    17641 gcccgcccga ttcaaaccgc gatgacgctt tgccgccggt tcggggcagg atggcggcat
    17701 ccgcgccgtc cgcaccgttt catttcacac cgcccttccg aaacccgccc gatccgacgt
    17761 gcggtatgaa acgcttgagc cggcgcgaag tcttctactg atttgcccgc gaaaataccg
    17821 tccgaaaggt tttcggacgg catttttttg cgttttcctg gatggcggcg gattcgtaaa
    17881 aggcgggcag ggtggattgc aggatgggtt gaggcctgcc aaatccgcca actctaccga
    17941 atacgccgcc gtcattcccg cgcaggcggg aatttggttt tgtcggtgcg gaaacttatc
    18001 ggataaaacg gtttcttaga ttccacgtcc tagattcccg cctgcgcggg aatgacggtt
    18061 cgggtatttc tgacgattca tcgagccgta gcaactgtat tttccac
//
LOCUS       DAXFZR010000047        17928 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000047 DAXFZR010000000
VERSION     DAXFZR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17928
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.047"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(302..409)
                     /locus_tag="ACFJAS_001337"
                     /pseudo
     CDS             complement(302..409)
                     /locus_tag="ACFJAS_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            431..925
                     /locus_tag="ACFJAS_001338"
     CDS             431..925
                     /locus_tag="ACFJAS_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982575.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKDLFAEEVPENNIVCDISHFLHFADRA
                     EADIPNQSRLYAEYWWEMETFKMSVLKDWERSGRPLDWEKWQRQYQSEAFELVKNLNR
                     SKQE"
     gene            1045..2178
                     /locus_tag="ACFJAS_001339"
     CDS             1045..2178
                     /locus_tag="ACFJAS_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982576.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRGLSH"
     gene            2185..2505
                     /locus_tag="ACFJAS_001340"
     CDS             2185..2505
                     /locus_tag="ACFJAS_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982577.1"
                     /translation="MPSETGFQTALESQTLFAMPYMPNPVRYKLKTTIQKTEIHIIKQ
                     TNKLTYCAILRAVPYFPVLYMSGRHPHKRRDTDKEKSLGIKNILINQYKSNKQFIIFF
                     SVGV"
     gene            complement(2681..2956)
                     /locus_tag="ACFJAS_001341"
     CDS             complement(2681..2956)
                     /locus_tag="ACFJAS_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC3982578.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(2953..4236)
                     /locus_tag="ACFJAS_001342"
     CDS             complement(2953..4236)
                     /locus_tag="ACFJAS_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3982579.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFMRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(4385..5803)
                     /gene="glnA"
                     /locus_tag="ACFJAS_001343"
     CDS             complement(4385..5803)
                     /gene="glnA"
                     /locus_tag="ACFJAS_001343"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC3982580.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGPAFDGSSIGGWKGIEASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            5826..6035
                     /locus_tag="ACFJAS_001344"
     CDS             5826..6035
                     /locus_tag="ACFJAS_001344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982581.1"
                     /translation="MAKYAERGGLTQSRHDFTTPMPSETGFASNSKPENIVSNGKIVN
                     NMLRFVCSKAECPPFCPNADKTDAV"
     gene            6114..6923
                     /gene="aroE"
                     /locus_tag="ACFJAS_001345"
     CDS             6114..6923
                     /gene="aroE"
                     /locus_tag="ACFJAS_001345"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC3982582.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            6926..7627
                     /gene="mtgA"
                     /locus_tag="ACFJAS_001346"
     CDS             6926..7627
                     /gene="mtgA"
                     /locus_tag="ACFJAS_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC3982583.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(7901..7976)
                     /locus_tag="ACFJAS_001347"
     tRNA            complement(7901..7976)
                     /locus_tag="ACFJAS_001347"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7941..7943),aa:Phe,seq:gaa)
     gene            complement(8167..8901)
                     /gene="lptB"
                     /locus_tag="ACFJAS_001348"
     CDS             complement(8167..8901)
                     /gene="lptB"
                     /locus_tag="ACFJAS_001348"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC3982584.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(8972..9502)
                     /gene="lptA"
                     /locus_tag="ACFJAS_001349"
     CDS             complement(8972..9502)
                     /gene="lptA"
                     /locus_tag="ACFJAS_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC3982585.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(9483..10064)
                     /gene="lptC"
                     /locus_tag="ACFJAS_001350"
     CDS             complement(9483..10064)
                     /gene="lptC"
                     /locus_tag="ACFJAS_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC3982586.1"
                     /translation="MKVRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(10061..10597)
                     /locus_tag="ACFJAS_001351"
     CDS             complement(10061..10597)
                     /locus_tag="ACFJAS_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC3982587.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(10663..11637)
                     /locus_tag="ACFJAS_001352"
     CDS             complement(10663..11637)
                     /locus_tag="ACFJAS_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC3982588.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            11734..12789
                     /gene="tal"
                     /locus_tag="ACFJAS_001353"
     CDS             11734..12789
                     /gene="tal"
                     /locus_tag="ACFJAS_001353"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC3982589.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEALARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            12801..12935
                     /locus_tag="ACFJAS_001354"
     CDS             12801..12935
                     /locus_tag="ACFJAS_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982590.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGSALKILMGKKLMHDITRQHKRK
                     "
     gene            12940..13230
                     /locus_tag="ACFJAS_001355"
     CDS             12940..13230
                     /locus_tag="ACFJAS_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982591.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            13247..14134
                     /gene="gluQRS"
                     /locus_tag="ACFJAS_001356"
     CDS             13247..14134
                     /gene="gluQRS"
                     /locus_tag="ACFJAS_001356"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC3982592.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(14204..15220)
                     /gene="dusA"
                     /locus_tag="ACFJAS_001357"
     CDS             complement(14204..15220)
                     /gene="dusA"
                     /locus_tag="ACFJAS_001357"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC3982593.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKVAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            15340..16494
                     /locus_tag="ACFJAS_001358"
     CDS             15340..16494
                     /locus_tag="ACFJAS_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3982594.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKMGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLGIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(16544..16810)
                     /locus_tag="ACFJAS_001359"
     CDS             complement(16544..16810)
                     /locus_tag="ACFJAS_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFC3982595.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(16918..17169)
                     /locus_tag="ACFJAS_001360"
     CDS             complement(16918..17169)
                     /locus_tag="ACFJAS_001360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982596.1"
                     /translation="MQEPAPPKEKEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGW
                     PHAEFDGFCDRLCNLIQAEKYGKKHYFPCSLPLFGGAAG"
     gene            complement(17176..17865)
                     /locus_tag="ACFJAS_001361"
     CDS             complement(17176..17865)
                     /locus_tag="ACFJAS_001361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC3982597.1"
                     /translation="MTFQGHNNRKKAGGYAEYITGGSLRRLVAAKVRRYCGEHPGVFD
                     GAAGSGQLEQYIEPSDFRAVEIQAEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVM
                     NPPFSIKLKDLSEDEKSRIAQEYPWKKSGVADEIFVLKGLENARRFGFFILFPGIAYR
                     KSEQRFREIIGNRLAELNRIQNAFEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLL
                     AADTWQIEPDL"
BASE COUNT         4147 a         4729 c         4813 g         4239 t
ORIGIN      
        1 tattttttcg agataataga tgacagttat atggactggt tgaaagaaga aagttttggt
       61 ttttttgaaa agaaatatta taaggcttat attttctttt ttagcgattc tgtaatagaa
      121 gttatcagct cgacagaacc tgtattttat tcaaaataac aaattatcaa acaaagctct
      181 gattaaaaac ccaacaatca aaatgccatc tgaagcggta ttcggctttc agacggcatt
      241 ttctccgatt ggcacaggat gcaggggctt gccggcttga ttttgccgaa gttgttggct
      301 cgtatagtgg attaaattta aaccagtaca gcgttgcctc gccttgccgt actatctgta
      361 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactataa tttggaagag
      421 gcagcaacct atggaaaata gatttgaaaa aatcgttaag gatattttta gttttaccca
      481 atttgtttcc gaagcggaaa ataaaattcc taaccaatcc tcccgttatt acgactgctg
      541 gtgggaattg gaaatcctga atgcctccgc attggaagat tgggaaatat cgggaaagcc
      601 tgaaaaatgg gagaaatgga cagaaaaata tcagaccgaa gcgttacagt cagttaagga
      661 tctatttgcc gaagaagttc ctgaaaataa tatagtttgc gatatttctc attttttgca
      721 ttttgccgat cgggcagaag ccgacattcc caatcaatca cgtttatatg ccgaatattg
      781 gtgggaaatg gaaacgttca aaatgtcggt tttaaaagat tgggagcgtt caggcagacc
      841 actagattgg gaaaaatggc aaaggcaata tcaatccgaa gcatttgaat tagtcaaaaa
      901 tttgaaccga agcaaacaag agtaacaagc cgcagcctgc ataggggttt ttaaagtacg
      961 gcagcaaccg tagattagat taaaaatcaa acaatcaaag cccgcctgat ataatcgata
     1021 tatcctgtta aacgaaaaat aaatatgttt aaaaaattca aaccggtatt gttgtcattt
     1081 tttgcacttg tatttgcctt ttggctggga acgggcattg cctatgagat taatccgcgt
     1141 tggtttttga gcgatacggc aactgaaata cctgaaaatc cgaatacttt tgtggcgaaa
     1201 cttgcccgcc tgttccgaaa tgccgacagg gcggttgtca tcgtgaagga atcgatgagg
     1261 acggaggaaa gccttgccgg agctgtggat gacggtccgt tgcagtcgga gaaggattat
     1321 cttgcactcg ctgtccggct cagtcgtttg aaagaaaagg cgaaatggtt tcacgtaacg
     1381 gagcaggaac atggggaaga ggtctggctg gattactata tcggcgaggg cggtttggtt
     1441 gcggtttcgc ttttgcaacg atcgccggaa gcgtttgtta atgccgaata tctgtatcgg
     1501 aacgatcgtc cgttttctgt aaatgtgtac ggcggaacgg ctcacgggga aaattatgaa
     1561 acgacaggag aatatcgggt tgtttggcaa ccggacggtt cagtatttga tgcgtcgggg
     1621 cgcgggaaaa tcggggaaga tgtttatgag cattgcctcg ggtgttatca gatggcccag
     1681 gtatatttgg cgaaataccg ggatgtcgcg aatgacgagc agaaggtttg ggacttccgc
     1741 gaagagagca accggattgc gtcggactcg cgcgattatg tgttttatca gaatatgcgg
     1801 gaattgatgc cccgggggat gaaggcgaac agtcttgtgg tcggctatga tgcggacggt
     1861 ctgccgcaaa aagtctattg gagtttcgac aatggaaaaa aacgccagag tttcgaatat
     1921 tatttgaaaa acggaaatct ttttattgca caatcttcga cggtagcatt gaaagcggat
     1981 ggcgtaacgg cggatatgca gacctatcat gcgcaacaga cgtggtattt ggatggcggg
     2041 cggattatcc gcgaagagaa acagggggac agactgcctg attttccttt gaacttggaa
     2101 gatttggaaa aagaggtgag ccgttatgca gaggctgcgg cgagacgttc gggcggcagg
     2161 cgcggccttt ctcactgaac gcaaatgccg tctgaaaccg gttttcagac ggcattggag
     2221 agtcaaacgc tttttgcaat gccgtatatg ccgaacccgg ttcgctacaa attgaaaacc
     2281 acaatccaaa aaacggaaat acacattatc aaacaaacaa ataagttgac atattgtgcg
     2341 atattgaggg ctgtaccgta ttttcccgtt ctttatatga gcggcaggca tcctcataaa
     2401 agaagggata ccgataagga aaaatccctc ggtataaaaa atatactgat taatcaatac
     2461 aaatccaaca aacagttcat cattttcttt tctgttggcg tataaaaacc gctcggaaaa
     2521 aaataaaata tcaaaacggc aaaataaaaa tactgatata tgccttcact ttcatagggc
     2581 gacgctctat gtgccgtcct gtgcgttgaa acatcggttt tccttgccgc ctgtcccgct
     2641 tttccctgat tgctgccaac tgccgtccga acctgaaccg tcaggtttca gacggcattt
     2701 tttctgcctg ctcccacaat tgccgttttt gtgcggcggt cagttttttg acggcgattt
     2761 cctgtttgag cgcagtgcct ttatccatgc cgcctgcaac gatacgcatc gccaccggtt
     2821 tgaatacgcg ggtatatttc gcgccttttc cggctgtgtg gatggcaagc cgctgttgcg
     2881 gattcgggct gataccgcaa tagaacgcgc tgttttcaca caatatcagg taaacgctcc
     2941 agtttgacgc gttcatctgc ctgcatcctg ggttttttcc ccgttccaag gcgcaacttt
     3001 cagcagcagc agcatacccg gcagggcaag tatgaaacac agccggaaaa acggtacata
     3061 acccatccat tcgatcagat agcctgcaaa ggaattgatg accgtgcgcg gcacggcgga
     3121 caggctggta aacagcgcaa gctgcgttgc cgtaaacgcg ggattggtct cgcgcgccat
     3181 atacgataca aacgccgccg tccccaaccc cacgccgacc gcttccgcgc cgataactgc
     3241 cgccagcatc agcctctcgc ctgtgccgac cgtgtcgaaa tgtccgaacc ctgccagcca
     3301 tacaaacccc aaaaccgtta ttgcctgcac cgcgccgaat agccacaagg ctttgtttac
     3361 gccgattttc agcatccaca cgccgcccaa gatacctgcc gccactgccg gccacagtcc
     3421 tgcatttttg gcaatcaaac cgatgtcggt cttgctgaaa cccatatcca gataaaacgg
     3481 cgttgccaac gcggttgcca tactgtcgcc gagtttgtaa aggaagataa acagcagcac
     3541 gcacaccgcc gaagcgatgc ccttgcgcat aaaaaattct ttaaacggct ctaccacggt
     3601 ctgcttcaac gttttaggaa cggaaggcgg caacacgggt tcgtgcgcga gaaacagcgt
     3661 catcagaagg ccgggcagca taaataatga agtgataaca aatacttctg accacggcat
     3721 cctgtctgcc aacaccaaac tcaatgaacc gggaatcagg gcggcaaccc ggtaggcgtt
     3781 cacatgaacc gagttgccca aacccaattc ttcgtccgac aaaatctcgc gcctgaacgc
     3841 atccaataca atgtcctgac tggcggaaaa aaaagcgaca agcaccgaca ggccggcaat
     3901 caacggcaga tgattccggg ggtttaaaaa ggcatatgcc gccaaagccg ccagtaaccc
     3961 tgcctgcgtc agcagcatcc acccgcgccg ccgtcccaaa acgggcagcc tgaccgcgtc
     4021 catcagcggc gaccacaaaa atttccaagt aaacggcaga ccgatcaacg ccatcagccc
     4081 gatgctcttc aaatccacct gctcgctgcg caaccacgcc ggaatcaggt taatcagaaa
     4141 gtacagcggc agccccgagg caaaccccgt aaaaatacag acaagcatat tgcgggaaaa
     4201 gatttgcccg ataaaacctg atttcgatgc agtcatcgtt cgtacagcca aaaaacaaaa
     4261 gcctattgta gcaaatatcg cccgcctgct gaaaaatgcc gccacgcgcc gccggtttgc
     4321 cgttccaatg caaaatgccg tctgaaactg tgttcagacg gcattgctac tgaaacccga
     4381 cggtttacag gctgtaatac atttcaaatt ccagcgggtg cggcgccata cggatgcggc
     4441 ggacatcttc ctctttaaag gcgatgtagc tgtcgatcca gtctttgctg aacacgccgc
     4501 cgcgcagcag gaattcgtgg tcggccttga gggcggcaag tgcttcttcc aaagaagcgc
     4561 agacggtcgg gacgagcgcg tcttcttccg gcggcaggtc gtacaggttt ttatcggcag
     4621 ggtcgcccgg atggattttg ttttgaatgc cgtccaaacc ggccatcagc agggcggcaa
     4681 atgccaaata cgggttggcg gtcgggtcgg ggaaacgcgc ttcgatgcgg cgcgccttgc
     4741 tgctgttcac agacgggata cggatggaag cggaacggtt tttggcggaa taggccaatt
     4801 tggtcggtgc ttcaaagtgc ggcacgaggc gtttgtagga gttggtggac ggattggtaa
     4861 tcgcgttcag ggctttggcg tgtttgatga tgccgccgat gtagtagagc gcggtatcgg
     4921 acaaaccggc atagccgtcg cctgcgaaca ggttttgacc gtctttccaa atggattggt
     4981 ggacgtgcat accgctgccg ttgtcgccca taatcggttt gggcataaag gtggcggttt
     5041 tgccgaaatt gtgggcaacg ttttggatga cgtatttcat atcttgggtt tggtcggcgc
     5101 gtttgaccaa agtggcgaaa cgggtgccga tttccatttg gctgccggta ccgacttcgc
     5161 tgtggtggac ttcgacttcg atgccgagtc cttccaaaat gttcaccatg gcggagcgca
     5221 aatcttgacc gcagtcaatc ggcgcgacgg gcgcgtagcc gcctttgacg gcggggcggt
     5281 ggccggtgtt ttgaccgtcc atatgcaggc cgcttgccca cgcgccgctt tcggacgtga
     5341 tttcgtaacg ggttttgtgc atgtcggttt caaattctac gccgtcgaag acgaagaatt
     5401 cgggttcggg gccgaaatag gcggtgtcgc cgataccgga agatttcaaa taggcttcgg
     5461 cgcggcgtgc gatggagcgc gggtcgcggt cgtaaccctg accgtcggca gggtcgatga
     5521 cgtcgcaggt aatgacgacg gtaacatcat cataaaaagg atcgacgaag gctgtggacg
     5581 catcgggacg cagctgcata tcggaagcct caatgccttt ccagccgccg atggacgagc
     5641 cgtcaaacgc cggtccgttt tcaaaccact cttcggggtc ttccaacacg atgcgcgcag
     5701 gcacggtaaa gtggtgctgc ttgcctttgg tatcggtgaa gcgcaaatcg acaaagcggg
     5761 cttcgctttc ttcaatcaat tttacggcgt ttttaatgga catcttcagc tcctgaaaaa
     5821 gggaaatggc aaaatatgcg gaacgcggcg gcttgacgca aagtcggcac gattttacca
     5881 cgccgatgcc gtctgaaacc ggttttgcgt caaattccaa gccggaaaat atagtttcaa
     5941 acggcaagat tgtcaacaat atgcttcggt ttgtctgttc aaaagcggaa tgcccgccct
     6001 tttgtccgaa tgccgacaaa acagatgccg tctgaaggcg gtttcagacg gcatcgttcc
     6061 catcttcgcg ccgccgttat aaaatgaccg ccccgccttt atggaatatg actatgcacg
     6121 ccctcccccg ctacgccgtt ttcggcaacc ccgccgccca cagcaaatcg ccgcagattc
     6181 atcagcaatt tgcccttcag gaaggcgttg acatcgaata cggacgcatt tgcgccgaca
     6241 tcggcggttt cgcgcaggcg gtttcgacat tttttgaaac aggcggttgc ggggcaaacg
     6301 ttaccgtacc gttcaaacag gaagcgtttc atctggcgga cgaacattcc gaccgcgctt
     6361 tggctgccgg tgcggtcaat acgctggtgt ggttggaaga cggcagaata cgcggcgaca
     6421 ataccgacgg tatcggtttg gccaacgaca tcacgcaggt caaaaacatt gccatcgagg
     6481 gcaaaaccat cttgcttttg ggcgcgggcg gcgcggtgcg cggcgtgatt ccggttttga
     6541 aagaacaccg ccctgcccgt atcgtcattg ccaaccgtac ccgcgccaaa gccgaggaat
     6601 tggcgcggct tttcggcatt gaagccgtcc cgatggcgga tgtgaacggc ggtttcgata
     6661 tcatcatcaa cggcacgtcc ggcggcttga gcggtcagct tcctgccgtc agccctaaaa
     6721 ttttccgcga ctgccgcctt gcctacgata tggtttacgg cgaagcggca aaaccgtttt
     6781 tagattttgc acgacaatcg ggtgcgaaaa aaactgccga cggactgggt atgctagtcg
     6841 gtcaggcggc ggcttcctac gccctctggc gcggtttcaa accggacatc cgccccgtca
     6901 tcgaacacat gaaagccctg taaccatgtt ccgcatcgtc aaatggctga ttgccctgcc
     6961 cgtcggcatc tttatcttct tcaatgccta tgtgtacggc aacatcatta cctaccgcgc
     7021 cgtcgcgccc catcggactg cctttatgtc gatgcggatg aagcagtttg aacaagaagg
     7081 tcgcgatgtc gcactggatt accgctgggt gccctacaac cgcatttcca ccaacctgaa
     7141 aaaagccctg attgcttccg aagatgtccg ttttgccgga cacggcggct tcgattgggg
     7201 cggcattcaa aacgccatca ggcgcaaccg gaacagcggc gaagtgaagg cgggcggatc
     7261 gaccatcagc cagcagcttg ccaaaaacct cttcctcaac gaaagccgca actatctgcg
     7321 caaaggggaa gaggcggcca ttacggcaat gatggaagct gttaccgaca aaaacaggat
     7381 tttcgaactg tatttaaact caatcgaatg gcactacggc gttttcggcg cggaagctgc
     7441 gtcccggtat ttttataaaa aaccggccgc agacctgacc aaacagcagg cggcgaaact
     7501 gacggcactc gtccccgccc cgctctacta cgctgaccat ccgaaaagca aacggctgcg
     7561 caacaaaacc aatatcgtgc tcagacgcat gggttcggca gaattacccg aaagcgatac
     7621 ggactgattg tttcagatat ggaaatgccg tctgaactgc ggttcggacg gcatatgttt
     7681 tctggttctt atagtggatt aaatttaaac cggtacggcg ttaccccgcc ttgccgtact
     7741 atgttgtact gtctgcggct tcgtcgcttt gtcctgattt ttgttaatcc actaacctgc
     7801 ccgacagccg caaagaccat gtcgtgcatg gcaggtattt gcggtgcggg actgctgccg
     7861 aaaacggcag gcaaaaaata accgcttata agcggttttt tggtggccag aggcggaatc
     7921 gaaccgccga cacacggatt ttcaatccgt tgctctacca actgagctat ctggcctgta
     7981 tctgtctcgt taagagatgc gtattaaacc aaatttcggg acgacacgca agcggatttg
     8041 tcgatatttt ttacccaacc gactaagtcg atgttttaag tttaaattaa tttcacagaa
     8101 aaatagtgca aatcggtttt ttgccgttcg ttcggcgatg ttctgctttg cagacggcaa
     8161 aacgcttcaa tatttgaagt tctcgcccaa atagacggcg cgcacctgtt cgttgccgac
     8221 caaatcgtcg ggcttgccgg aagccagcac cgcaccgtct gaaatgatgt aggcgcggtc
     8281 gcagatgctg agggtctcgc ggacgttgtg gtcggtaatc aatacgccga tgccgcgcga
     8341 tttgaggaaa ccgatgattt tctggatgtc gatgacggcg atgggatcga cgccggcaaa
     8401 aggttcgtcc aaaaggatga aacgtggttt catggcgagg acacgggcga tttcgacgcg
     8461 ccgccgttcg ccgccggaca gcgacggcgc aggattgcgg cgcaagtgtc cgatattgag
     8521 gtcggcgagc agtttttcga tttctctgtc gatttgattt ttatctttgg ttctgatttc
     8581 caagatggcg cggatgtttt gttcgacggt cattttgcgg aatatcgagg cttcctgcgg
     8641 caggtagccg acacccaggc gggcgcgttc gtgtatgggc aggtggcgca attcccgccc
     8701 gtccagcatc acgctgcctg cgtcggcagc gatgagcccg actatcatgt agaagccggt
     8761 ggttttgcct gcgccgttgg gcccgagcag tccgatgact tcgccgcttt cgatctcaag
     8821 ggagaaactt ttgacgactt ggcgtttttt gaaacttttc tgcaggtttt ggacgacgag
     8881 gcggctgttg tttgcgctca taaatctctt ctcggcattt cggtcggcaa atgccgtctg
     8941 aactgcgttt ccgtttcaga cggcatcggg gttattcggt tttttgtgtg cttgaaggct
     9001 ggatgacgac gctgaccctg ccggttttgg aagcggattt cgcacccgat ttcgtgctgc
     9061 cgttgatggt atagacttcg gttttggtgt tgtaggtaat gaccgcacct tcggcaacgt
     9121 cgccgccgcg ctgcactttg gcattgccgg tcagaacgac ggtgcttcct gcggaggaat
     9181 aggtaacgtt gtttgcctga ccgcgcaccg tccctttgcc cccgtccaac gtttggctga
     9241 agcggacggg cgaaccttcc gccctcacgg attcgccgcc tttgccgccg cgtgtgacgt
     9301 tgacgcgcga ggcggaaatg ttgagcgtac cctgtctgat gatgacattg ccgctaaatg
     9361 tggtactttg gttggcttga tcgagcgaac cttggtcggc ttcgatttgg atgggccgtc
     9421 tgctgtcgct ttgaagggca aaagcgggag aagttgcaaa aattacaatt aaaacaaata
     9481 gcttacatat cttttgtatc ataaatcgcg gctttcactt tagatgagaa gttcaacatg
     9541 cctgttttgt ggttgtaggt cataccgccc gcctgaccgt gcgatgcacc atattggaaa
     9601 ctgacaggcg tatcggtttg ggcatattga gattcggtat cgacgtgcag tttttcggtt
     9661 tcgactttac ccgcctgccg cctgccgtcg gcggttttgg tcagcacaac gttgttttta
     9721 aaaagaacct gtttgttttc ggtatggtaa acggcttcat cgctgccgac ttcgtacaac
     9781 agcctgcctt cttggaagaa cacgagatgc ggcgaatcaa aatggatgtc gctgttttcg
     9841 ggaaactgtt tcgcaccttt cgcgctcaaa tgttctttca agtatccctg ttcgtcaaac
     9901 cgccttccgt ccaagccgtc cattgtgtat tgaggttcgt cgggattgag cctgacttcc
     9961 tcgatttcga cttcgctgat acggcccaac catgccgaca ggccgcccaa ggcaaccgcc
    10021 aatatcaatg ggaacgcaat tccgtaccgc catcttactt tcatttgatg tacccgttca
    10081 aagccgcgcc caacgtacct ttcgcgcgga taatcaggtc acacacttcg cgcaccgcgc
    10141 ccgcaccgcc ggggcgtttg gcaatatagg cggcgtgttg cagcgtaaac caatgcgcgt
    10201 cggggacggc aaccggcaat ccgcagcgca ccatcaccgg caaatcgacc acgtcgtcgc
    10261 cgacaaaggc gcactcggct tcttccaagc ctgcctgcgc gcgcagctct tcatacgcgg
    10321 cgcgtttgtc cgaaatgcct ttgaagtagt aatttatgcc caactgtttg acgcggatgc
    10381 cgacggaggg cgcatcccga cctgtgataa tcgcagtctg cacgccgctt gcctgaagca
    10441 ttttcaggcc gtgtccgtcc agtgtgtgaa acgatttgat ttcttcgccg tcgtcgcgga
    10501 taaagatgcg cccgtcggtc aaaacgccgt ccacatccaa tatcagcagt ttgattttgg
    10561 cggcgcgtgc ctgtaattcg ggagaaattg cctgcattgg gatttccttg ttttcagacg
    10621 gcatatctta ccacaaatgc cgtctgaaac ggctgcaacg ccttatacaa tccgcgccgc
    10681 caaaaggtcg tgcatattca gcgcacctgt cagcacgccg tctgcatcgg taaccagaag
    10741 cccgttcaca tggtttgcct gcatgacttt cagggcttcg gtggcgaggc gttcggcgga
    10801 gatggtttta gggtgtgtat gcatgacttc gtctatcgaa agaccggtaa aattgtcgca
    10861 ttcttgaaac aggcggcgca aatcgccgtc agtaaacacg cctttcagac agccttgccc
    10921 gtccgttacc gccaacatgc ccaaaccttt ctcgctcatg ctgacgatgg ctcctttcaa
    10981 gggcgtgccg agtcggacgg cgggcaggcc gccgcctttg tgcataatgt cggcaacgcg
    11041 caaaagcagg cgtttgccga ggctgccggc agggtggatc aaggcgaagt cgtcgggcgt
    11101 gaacgcgcgg gcgcgcagca ggacgaccgc caacgcgtcg cccaaagcca taacggcggt
    11161 ggtgctggtg gtcggggcaa gccccaacgg gcaggcttct tgcgaaaccg atgcggtgat
    11221 gtggatatcg gcatggcgcg ccatggttga atcggggcgg gcggtgatgc agacgagggt
    11281 aatgtctttg cgtttgagcg cggggatgat ggcggtgatt tcgtcgcttt cgccggaatt
    11341 ggaaatggcg gcgacgacgt cgttgtccac aatcatgccc aaatcgccgt gtgccgcttc
    11401 cgcagggtgg acgaaaaacg cgggcgtgcc ggtcgaggcc atggttgccg ccattttgcg
    11461 cccgatatgt ccggacttgc ccatacccgt gatgacgacc ctgcccttgc agtgcaacaa
    11521 cgcgtctgcc gcaaggacga agttttcgtc caattccgcc gcaatttcgc gcaagccttc
    11581 cgcttcggtg tgcaacactt cgcgcgccca gtcgagatat ttttcgtttt ctgccattgc
    11641 catccgcctt tgctgtaaca aaactaccca aaatttccga atcggggtag cataagcctt
    11701 caaaccccgc ccatccatcc aaaggaatca aaaatgacta ttttatcgga cgttaaagca
    11761 ttaggacaac aaatctggct ggacaacctt tcccgctcgc tcgtgcaaag cggcgaattg
    11821 gcgcaaatgc tcaaacaagg cgtgtgcggc gtaacttcca atcccgccat tttccaaaaa
    11881 gccttcgccg gcgacgcgct ttatgccgac gaggtcgccg ccctcaagcg gcaaaacctc
    11941 agccccaaac aacgctacga aaccatggcg gttgccgacg tacgggcagc ctgcgacgtt
    12001 tgccttgccg aacacgaatc caccggcggc aaaaccggtt tcgtcagtct cgaagtttct
    12061 cccgaacttg ccaaagacgc tcaaggcacg gtagaagaag cccgccgcct ccatgccgcc
    12121 atcgcgcgta aaaacgccat gattaaagtg cctgccaccg acgcaggcat cgatgcgctc
    12181 gaaaccctcg tttcagacgg catcagcgtg aacctgaccc tgctgttctc acgcgcccaa
    12241 accctcaaag cctacgccgc ctacgcgcgc ggcattgcca aacgcttggc agccggacaa
    12301 agcgttgccc atatccaagt tgtcgccagc ttcttcatct cgcgcgtgga cagtgcgctg
    12361 gatgcaacgc tgcccgaccg gctcaaaggc aaaaccgcca tcgcccttgc caaagccgcc
    12421 tatcaagatt gggaacaata tttcaccgcc cccgaatttg ccgcactgga agcccaaggc
    12481 gcaaaccgcg tgcagctttt atgggcatct accggcgtga aaaacccagc ctatcccgac
    12541 acgctctacg ttgacagcct gatcggcgtg cacaccgtca acaccgtccc cgatgccacg
    12601 ctcaaagcct ttatcgacca cggcacggcg aaagcgacgc tgaccgaaag cgcggacgaa
    12661 gcactggcgc ggctcgccga aattgccgcg ctcggcatcg atgtcgaaac cttggcggcg
    12721 cgtttgcagg aagacggttt gaaacagttt gaagaagcct ttgaaaaact gctcgcgcct
    12781 ttggtttagg catttccccg atgccgtctg aaaggctttc agacggcatc gcccttttga
    12841 ccggacacag tgcggcatat caaggatccg ccctaaaaat attaatgggc aagaagttaa
    12901 tgcacgacat tacccgccaa cacaaaagga aatagaatca tggcaaaagc actcgaaatc
    12961 atttcacccg atgaaattta ttcagatctg atttttaagg atccggtacc tccccatact
    13021 atatatacag aattaatgaa actggttggt agaaatgcag gcaatgagag aattgcagat
    13081 agagccttcg attttttctc gcctgctctt atggatgatt cagctaccga agagcaatat
    13141 aatgccctat atgatttaac cttgcttgaa gagcccggga tggaactgga taaagatgag
    13201 ataacggctt tgattaatag tcttaaataa aggggctgta ccggatatgt acacaggacg
    13261 cttcgccccc agcccgaccg ggttgctcca catcggctct cttctgaccg ccgtcgcttc
    13321 ctatgccgat gcacgttcaa acggcggcaa atggctggtc cgtatggaag acctcgatcc
    13381 gccgcgcgaa atgccggggg cggcaagcca tatcctgcac acgctcgagg cattcggatt
    13441 cgagtgggac ggcgaagtca cctatcagag ccgccgttac gccctgtatg aagaaaccct
    13501 ataccgtctg aaaaccgccg gactggtcta tccctgccat tgcaaccgca aagactggca
    13561 ggccggggca aggcggggca cagacgggtt cgtctataac ggacgctgcc gccaccccgg
    13621 ccaacgccct gcactgcaag gcaaacagcc gtcgtggcgc atccgcgtcc ccgaccgcga
    13681 catcggcttt tcagacggca tcgtcggcag ttacacccaa aacctcgccc gcgacatcgg
    13741 cgatttcgtc ctgttccgtg cagacggtta ctgggcatac cagctcgccg tcgttgccga
    13801 cgatgccgaa cagggcgtta cccacatcgt gcgcggacaa gacctgctcg tttccacacc
    13861 gcgccaaatc tatttgcagc agtgtttggg cgttccgaca ccgcaatatg cccacctgcc
    13921 gctgctgacc aacgcgcaag ggcagaaatg gtcgaagcag acgctcgccc ccgcattgga
    13981 tttaaaccgc cgcgaacaac ttctccgcca agtgttccgc tacctcaagc tgcccgaagc
    14041 accggaaacc gaccgccctg ccgaattgct cgactgggcg gtggcacact gggatatgga
    14101 caaagtgccg aaacacgcca ttaccgcccc ctaaacacca cccgacaaac gcacaaatgc
    14161 cgtctgaaac cttcagacgg catttcacac atacagcccc gccctattcg cgcgtatttg
    14221 cccgttcgac ttctttccac gcttcgaaaa tcaggccgcc gtcgttgtct ttcaagagcg
    14281 ttgcatcgga aagcatacgc cgccaagtcc gcgcgccttt caaaccgtgc atcagcccga
    14341 gactgtggcg gacgatgtgg cgcaggattg tgccgcgtcc ggcttggatt tggacttggc
    14401 tgtatgtgta gaggcgctgc accaaatcgg cgtattcgat cggggcgcgg ttgtcaccgt
    14461 aaaacagcct gtcccattcg cgcatcacca tcgggttgtg gtatgcctcg cgcccgacca
    14521 tcacgccgtc aacgtgttgc aagtgtccgg cgattgcttc gttggtggtg atgccgccgt
    14581 tgatgatgat ttccagctcg ggaaactcct gcttgaggcg gtaaacgtaa tcgtatttca
    14641 agggcggaac atcgcggttt tctttgggcg aaagaccgtc cagccaagcg ttgcgggcgt
    14701 ggacgatgaa ggttttgcag gcggttttgt cgcgcagcgt gccgacgaaa tcggcaacgg
    14761 tttggtattc ggtctgcctg tccacaccga tgcggtgttt gacggtaacg gggatgccga
    14821 ccgcgtcgtg catggcgttg aggcagtcgg caaccagccc gacttcgttc atcagacacg
    14881 cgccgaacgc gcctttctgc acgcgcggac tgggacagcc gcagttgagg ttgacctcgt
    14941 tgtagccgta ttcttcggcg gctttggcga ctttcgccaa atcggacgga tcgctgccgc
    15001 ccagttgcag ggcgacgggc tgctcgcctt cattgaacat caaaaagcgg tctttgtcgc
    15061 cgtaaacaat cgcgccggcg ttgaccattt cgctgtacag ccaagcattt cgggtaatct
    15121 ggcgggcaag gtaacggtag tgcctgtccg tccagtcgag catcggggcg acgcataaac
    15181 gccttttccc tttatgttca ataagttcct tgtttatcat aaggttttca cattctttta
    15241 actcaaacat tctttattat ttgcttggat ttttgcccat ttcactatat ttgacgtacc
    15301 atacttgcac cataattttt aattcttgga atggtgcaaa tggcaacaat aaccaagcgg
    15361 cgcaatcctt ccggcgaaac agtatatcgg gttcaggtac gggtcggcaa gaaaggctat
    15421 cctgctttca atgagagcag gacgttcagt aaaaaggctt tggcggtcga atgggggaag
    15481 aaacgggagg cggaaatcga agccggtcca gaactgcttt tcaagcgcgg caaggtcaag
    15541 atgatgacgc tgtccgaagc tatgcggaag tatcttaacg aaacgcttgg agcgggtcgg
    15601 tcaaagaaaa tgggcttgcg ttttctgatg gagttcccga ttggcggcat cggcatcgat
    15661 aagctgaaac ggtctgattt cgcggaacac gttatgcaga ggcggcgcgg aatccccgaa
    15721 ctggacattg cgccaatcgc ggcttctacg gcattgcagg agctgcaata tatccgttcc
    15781 gtgctaaaac acgcctttta tgtgtggggg cttgaaatag gctggcagga attggatttc
    15841 gcggcaaacg ggctgaaacg ctcgaatatg gttgcgaaat ctgcaatcag ggacagattg
    15901 ccgaccacgg aagaactgca aaccctgaca acttatttcc tgcggcaatg gcaaagcagg
    15961 aaatcttcca taccgatgca cctgattatg tggctggcga tttatacgtc aaggcggcag
    16021 gatgagattt gccgcctgct gtttgacgac tggcacaaaa atgattgtac ccggccggtt
    16081 cgtgatttaa aaaatccgaa cggcagcaca gggaataata aggagtttga tattctgcct
    16141 atggctttgc cggtcattga cgagctgccg gaggaatcgg tcaggaagcg tatgcttgcc
    16201 aacaaaggca tcgccgacag ccttgtaccg tgcaacggaa aatcggtttc cgccgcttgg
    16261 acgagggcgt gcaaggttct tggaattaaa gacttgcgct tccacgattt acggcacgag
    16321 gctgctaccc gtatggctga agacggcttc acgattccgc aaatgcaacg ggtaacgctg
    16381 catgacggtt ggaacagcct gcagaggtat gtgagcgtac gcaaacgctc gacgcggctg
    16441 gattttaaag aggcaatgat gcaggcgcaa agcgatataa aatccgggaa gtgatattaa
    16501 attaaagggg atgtgccgca tcccctgatt cctccgtctt gcatcaagac cggaatttcc
    16561 gatattccga ttttgccttt tcctccttgt ctgcgattgc ctttgcaagc tctcggacgc
    16621 tgacgaaata ttccgatttt cggctgtcgg acaatcggaa aatcggaaac ggaagctcgc
    16681 accgcacccc cgatgcttta aacgcgttaa agccgatatg ggcaaaaaag tctttgtgta
    16741 cttccctcgg cgggatatac gggcggccgc cgtatgcgat taaaagccct tgttctttac
    16801 tgatgttcat atatacctct ttctgttaat tctgctttgt ccgggtttcc gcttttttac
    16861 ggcttggcgg atgttcagcc tgcgtcggtg cataagtcct cctgttcggg cggttgttca
    16921 gccggccgcc cctccgaaaa gtggcaggct gcacgggaag tagtgttttt ttccgtattt
    16981 ctctgcctgt atcaggttgc acaggcggtc gcaaaagccg tcaaattcgg catgcggcca
    17041 gccttctatc tcgttgacca ttttgctgaa cctcaattcc cgggcaatcc gctttgccgc
    17101 tgcgtcacgg cattcgtgtt ccaagaggac ggggtcttcc ttttccttcg gcggtgcagg
    17161 ttcttgcacg gtttgctaca agtccggttc gatttgccaa gtgtcggcgg caagcagggt
    17221 gtctgttttg cagtcgtaca gttcgcggat gcagccgccg tcggttttgt ctttgtcgat
    17281 aaccagtaaa agcacttcga tcggcgtgtc ttcaaacgcg ttttgtatgc ggttgagttc
    17341 tgccagccgg ttgccgatga tttcgcggaa tcgctgctcg cttttgcggt aggcgatgcc
    17401 ggggaacagg atgaagaatc cgaagcggcg ggcgttttcc aatcctttta aaacgaagat
    17461 ttcatcggct acgcccgatt tcttccacgg gtattcctgt gcgatacggc ttttttcgtc
    17521 ttcgctcaaa tcctttagct tgatggaaaa cggcggattc atgacggtgc agtcttgcgg
    17581 ttcgccgtcg gtataaagga aaaggcttgt gttgtatact ttggcggcag ggtagttttg
    17641 caacaaggct ttacacgctt ccgcctgtat ctctacggcg cggaaatcgg acggttcgat
    17701 gtactgctcc agctgcccgc ttcctgccgc gccgtcaaat acgcccgggt gttcgccgca
    17761 gtatcggcgg actttggcgg caaccagccg cctcaagctg ccgcccgtga tgtattcggc
    17821 gtagccgccg gcttttttac ggttgttgtg tccttgaaac gtcatgggtt ttcgtccgtt
    17881 ctctggcttg tccgggtgtg gcggagcgtt tgcggatgcg gctgacgc
//
LOCUS       DAXFZR010000048        17407 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000048 DAXFZR010000000
VERSION     DAXFZR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17407
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.048"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..486
                     /locus_tag="ACFJAS_001362"
     CDS             <1..486
                     /locus_tag="ACFJAS_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC3982598.1"
                     /translation="SLLLPENYAAVRFTVVSCMLPPLFCTLAEISGIGLNVVRKTRPI
                     AFATLGALAANLLLLGLSVPAGGARGAAVACAASFWLFFVFKTESSCRLWQPLKRLPL
                     YMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAGCILRHRKNLHKLFHYLKKQGFP
                     L"
     gene            483..1548
                     /locus_tag="ACFJAS_001363"
                     /pseudo
     CDS             483..1548
                     /locus_tag="ACFJAS_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            1562..2734
                     /locus_tag="ACFJAS_001364"
     CDS             1562..2734
                     /locus_tag="ACFJAS_001364"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC3982599.1"
                     /translation="MNITIAAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVILLEESGYSKNVSLGRVTSHHRFVKHFEKWLENCRPGE
                     QDVVYSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKETNPNVPGEVIYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLDRLKQYACDGIKFYGY
                     IPYAEMMSVAKGCDIAVNAIRSYSMASITNKLSDYMALQKPILNSQVNDEVAEVLTLL
                     PHANYRSGDVDGFVRAAKDILKRKNDPVQSEEIVRRFKPDVAYQKIVNLIERLADE"
     gene            2727..3968
                     /locus_tag="ACFJAS_001365"
     CDS             2727..3968
                     /locus_tag="ACFJAS_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC3982600.1"
                     /translation="MNKFFKRLIDIVASASGLIALSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGPRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSATIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            4099..5274
                     /locus_tag="ACFJAS_001366"
     CDS             4099..5274
                     /locus_tag="ACFJAS_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC3982601.1"
                     /translation="MLNTALSPWPSFTREEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            5322..7232
                     /locus_tag="ACFJAS_001367"
     CDS             5322..7232
                     /locus_tag="ACFJAS_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFC3982602.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVKITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            7288..8580
                     /locus_tag="ACFJAS_001368"
     CDS             7288..8580
                     /locus_tag="ACFJAS_001368"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC3982603.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(8632..9468)
                     /locus_tag="ACFJAS_001369"
     CDS             complement(8632..9468)
                     /locus_tag="ACFJAS_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC3982604.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKRQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(9763..9966)
                     /locus_tag="ACFJAS_001370"
     CDS             complement(9763..9966)
                     /locus_tag="ACFJAS_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC3982605.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(9944..10345)
                     /locus_tag="ACFJAS_001371"
     CDS             complement(9944..10345)
                     /locus_tag="ACFJAS_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC3982606.1"
                     /translation="MQPAVGQDFFGRMAAYGVSDGIFTEQAVRTHRRFCGNFGCGGGR
                     CYNNALFLKGDIMEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNGGT
                     EIRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            10710..14144
                     /gene="dnaE"
                     /locus_tag="ACFJAS_001372"
     CDS             10710..14144
                     /gene="dnaE"
                     /locus_tag="ACFJAS_001372"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC3982607.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            14186..14440
                     /locus_tag="ACFJAS_001373"
                     /pseudo
     CDS             14186..14440
                     /locus_tag="ACFJAS_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14796..15727)
                     /locus_tag="ACFJAS_001374"
                     /pseudo
     CDS             complement(14796..15727)
                     /locus_tag="ACFJAS_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(15797..16340)
                     /locus_tag="ACFJAS_001375"
                     /pseudo
     CDS             complement(15797..16340)
                     /locus_tag="ACFJAS_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(16286..16501)
                     /locus_tag="ACFJAS_001376"
     CDS             complement(16286..16501)
                     /locus_tag="ACFJAS_001376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982608.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            16661..17320
                     /locus_tag="ACFJAS_001377"
     CDS             16661..17320
                     /locus_tag="ACFJAS_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC3982609.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
BASE COUNT         4001 a         5225 c         4564 g         3617 t
ORIGIN      
        1 tccctcctac tgccggaaaa ctacgccgcc gtccggttta ccgtcgtatc gtgtatgctg
       61 ccgccgctgt tttgcacgct ggcagaaatc agcggcatcg gtttgaacgt cgtccgcaaa
      121 acgcgtccga ttgcattcgc caccttgggc gcgctggcgg caaacctgct gctgctgggg
      181 ctttccgtac cggccggcgg ggcgcggggc gcggcggttg cctgtgccgc ttcattctgg
      241 ctgttttttg ttttcaagac cgaaagctcc tgccgcctgt ggcagccgct caaacgcctg
      301 ccgctttata tgcacacatt gttctgcctg gcctcctcgg cggcttacac ctgcttcggc
      361 acgccggcaa actaccccct gtttgccggc gtatgggcgg catatctggc aggctgcatc
      421 ctgcgccacc ggaaaaattt gcacaaactg tttcattatt tgaaaaaaca aggtttccca
      481 ttatgaaaat catcctgact acttctatgt cgggtctggg cggcacagaa accgccaccg
      541 tccgcctcgg gcggctgctc aaacggcacg ggcacgacat catcctcgcc tcctcggacg
      601 gcccttttgt cggagaggcg caagcatcgg gcatccgttg gcagccggtt gatttttacc
      661 ggggcgggct tgccggctac cttaaaagca cctttgccta cgcccggatg ctgcgccgcg
      721 aacaacccga catcatcgac tgccaaatgg cgcgcgtcgt cccggcctgc gcccttgccg
      781 ccaaaatcgt ttcaccgaaa accaaaatca tctgccactc gcacggtttg gacgcggcaa
      841 cctaccccaa aaccgccaaa ctcttcgaca agctgggcgc gtacatcatc ggcaactgca
      901 aacacgaacg cgaaaagctc atccgccacg gcttccccgc cggccggatt gcctacgcct
      961 acaacacccc ccccggaatt tcatttccgg aaaaccgaaa aagaatgcgc cgtactcggc
     1021 acactttccc gtttggacac cgtccgcgcc gtgcatctga tgttggatat tttgaagaaa
     1081 atggttggcc gcaatatacc cgtacgcctg aacatggcgg gcataggaga agaaatggac
     1141 aacctcaaag cccaagccaa acgtttgggt attgaagaca aagtgacctt cctcggcggc
     1201 gtccgcgatt tgaccggcta cttcaaagaa gtcgatattt tagtgaacac gccgcattgc
     1261 gtaggtgacc acggcgcagg tgtcggcaac aatattttgg aagccggcct ttacgacacg
     1321 cctgtcgtga cctacaacat ggcgggcatt tccgaaatgg tcattaccgg ccaaaccggc
     1381 tactgcattc ctttcggcga tgacgaagcg tttatcgaag ccgtcgatac actcatcaag
     1441 catccagagt tgcgcagcca aatgggcaaa gccctgcaca aacatgtcga aaccttatgc
     1501 tccgacgacg aaatctaccg caccaccatg gctgcgtacg aaatgtaagg ccgtctgaaa
     1561 catgaacatt accatagccg ccccctactg ctcgctgccg tccgaaccgc atttcaaccg
     1621 cttttggtat ctggcagaac tattgtcgca atcgcacgac gtgttgctga ttaccagcaa
     1681 cttcaagcac tacgacaaat ctttcagacg gcccgaagat gccgaggccg cctcacaagg
     1741 ccgtctgaaa gtcatactgt tggaagaaag cggatatagc aaaaacgtat ctttgggacg
     1801 cgtgaccagc catcaccgct tcgtcaaaca ttttgaaaaa tggttggaaa actgccgtcc
     1861 gggcgaacaa gacgtcgtct attccgccta tccgctgatt gccaccaacc tgctgttggg
     1921 caaacacaaa gcgcgtttgg gctacaaact gattgtcgat gtgcaggacg tatggccgga
     1981 gtctttctcc tcggtcgtac cgtttttgaa aaaagtaccg cacaagctgc tgccctttgc
     2041 ttcacgcgcc aaccgggcct accgctacgc cgacgcgctg gttgccgtat cgcagactta
     2101 tctcgaccgc gccaaagaaa ccaatccgaa cgtacccggc gaagtcatct atattggtgc
     2161 tgactttccc aaactcgatg ccgcacctgc caaagatttt ggcgacgaca aaacccgctt
     2221 tttctacttg ggcacgctca gttacagcta tgacgtggaa accgtgtgca aaggcgttcg
     2281 gaaacttttg gacgacggcg aaaatgtaga gctgcacatt atgggcggcg gccccgattt
     2341 ggacaggctc aaacaatatg cctgcgacgg catcaagttt tacggctaca ttccttacgc
     2401 cgaaatgatg tcggtcgcca aaggctgcga cattgctgtc aacgccatcc ggtcctattc
     2461 aatggcatcg attaccaaca aactctccga ctatatggct ttgcaaaaac cgattctgaa
     2521 cagccaggtc aacgacgaag ttgccgaagt ccttaccctg ctgccgcatg cgaactaccg
     2581 ttccggcgat gtggacggtt tcgttcgagc cgccaaagat attttgaagc gcaaaaacga
     2641 tcctgttcag tccgaagaaa tcgtccgccg tttcaagccc gacgttgcct atcaaaaaat
     2701 cgtcaacctg attgaaagat tagctgatga ataaattttt caaacgcctc atcgacattg
     2761 tcgcctccgc atcggggctg attgccctgt cgcccgtgtt tttggtttta atatacctca
     2821 tccgcaaaaa cttaggttcg cccgtcttct tcattcggga acgccccgga aaggacggaa
     2881 aaccttttaa aatggtcaaa ttccgttcca tgcgcgacgc gcttgattca gacggcattc
     2941 cgctgcccga cagcgagcgg ctcacgccct tcggcaaaaa actgcgcacc gccagtttgg
     3001 acgaactgcc tgaactgtgg aacgtcctca aaggcgagat gagcctggtc ggcccccgcc
     3061 cgcttttgat gcagtatctg ccgctttaca acaaatttca aaaccgccgc cacgaaatga
     3121 aaccgggcat taccggctgg gcgcaggtca acgggcgcaa cgcgctttcg tgggacgaaa
     3181 agttctcctg cgatgtttgg tacaccgaca atttcagctt ttggctggat atgaaaatcc
     3241 tgtttctgac agtcaaaaaa gtcttgatta aagaaggcat ttcggcgcaa ggggaagcca
     3301 ccatgccccc tttcgcgggg aatcgcaaac tcgccgttat cggcgcgggc ggacacggca
     3361 aagtcgttgc cgagcttgcc gccgcactcg gcacatacgg cgaaatcgtt tttctggacg
     3421 accgcaccca aggcagcgtc aacggcttcc ccgtcatcgg cacgacgctg ctgcttgaaa
     3481 acagtttatc gcccgaacaa ttcgacatca ccgtcgccgt cggcaacaac cgcatccgcc
     3541 gccaaatcac cgaaaaagcc gccgcgctcg gcttcaaact gcccgttctg attcatcccg
     3601 acgcgaccgt ctcgccttct gcaacaatcg gacaaggcag cgtcgtaatg gcgaaagccg
     3661 tcgtacaggc cggcagcgta ttgaaagacg gcgtgattgt gaacactgcc gccaccgtcg
     3721 atcacgactg cctgcttgac gctttcgtcc acatcagccc gggcgcgcac ctgtcgggca
     3781 acacgcgtat cggcgaagaa agccggatag gcacgggcgc gtgcagccgc cagcagacaa
     3841 ccgtcggcag cggggttacc gccggtgcag gggcggttat cgtatgcgac atcccggacg
     3901 gcatgaccgt cgcgggcaac ccggcaaagc cccttacggg caaaaacccc aagaccggga
     3961 cggcataagc gattaaaata aacttccgta caaaccgatt ttgacaacac ctgcggcgcg
     4021 cgcccgattc ttcggaacac gcccccttca gacggcatag ggtcggaaat gccgtccgaa
     4081 cccgacggac caaacaccat gctgaacacc gccctttccc catggccgag cttcacccga
     4141 gaagaagccg atgccgtttc caaagtcctg ctgtccaaca aagtcaacta ctggacgggc
     4201 agcgaatgcc gcgaatttga aaaagaattt gccgcctttg ccggcacgcg gtacgccgtc
     4261 gccctttcca acggcacgct tgcgctcgat gccgcactca aagcaatcgg cataggcgcg
     4321 ggcgacgatg tgattgttac ctcgcgcacc ttcctcgcct ccgcctcctg cattgtgaac
     4381 gcgggcgcaa accccgtgtt tgccgacgtg gatttgaaca gccaaaacat cagcgcggaa
     4441 accgtcaaag ccgtgctgac cccgaatacc aaagccgtca ttgtcgtcca ccttgccggt
     4501 atgcctgccg aaatggacgg catcatggct ttggcaaaag aacatgattt gtgggtgatt
     4561 gaagactgcg cccaagcgca cggcgcgacc tacaaaggca aatccgtcgg ctctatcggg
     4621 cacgtcggcg cgtggtcgtt ctgtcaagac aaaatcatca ccaccggcgg cgagggcggt
     4681 atggttacga ccaacgacaa aaccctgtgg gaaaaaatgt gggcgtacaa agaccacggc
     4741 aaaagctacg atgccgtgta ccaccgcgaa cacgcgcccg gtttccgctg gctgcacgaa
     4801 agtttcggca ccaactggcg catgatggaa atgcaggctg tcatcggccg catccagctc
     4861 aaacacctgc ccgaatggac ggcgcgccgc caagaaaacg ccgccaagct ggcagaaagt
     4921 ttgcgcaaat tcaaaagcat ccgcctgatt gaagtcgccg gctacatcgg acacgcgcaa
     4981 tacaagttct acgccttcgt caaacccgaa cacctgaaag acgactggac gcgcgaccgc
     5041 atcgtcagcg aactgaacgc gcgcaacgtc ccctgctatc aaggcggctg ctccgaagtg
     5101 tatttggaaa aagccttcga caacacgccg tggcggccca aagagcggct gaaaaacgcc
     5161 gtcgaactgg gcggcaccgc cctgacgttc ttggtgcacc cgacgctgac cgacgacgaa
     5221 atcgcgttct gcaaaaaaca catcgaagcc gtcctgaccg aagccgcacg ataacccttc
     5281 agacggcata tgccgcctga aagcacatac cgccccacga tatgaatctg gaaaccctga
     5341 tcgccctgcc gcgcaacatc aagaaaatct gtttcctcat acacgatttt ctgatgattt
     5401 tcattgcctt ttggttcacc caaagcctga aggccgacta ctcggacgaa tggttcgatt
     5461 ttgccaactg gcagtctttt ttgctgactg ccttgctgac catcacatta tttatccgaa
     5521 tggggcttta ccgcgccgtt acacgctttg tcagtttcca cgtcctgacc accgcctttg
     5581 ccggcagcct cgcctccgcc gtattgttct tcctcaacac attgattttt gaagaaaggc
     5641 tgcgcctcgc cctgccgatt gtctatttct tactgctgtt tgtttccgtg accggctcgc
     5701 gtatggtttt gcgcggactc ctgtccgacc accagaaaaa aacacatgac cccgtcatca
     5761 tttacggcgc ggggcgttcg ggtcggcagc tccttgaagc cgtcaaacag atgcgcgaat
     5821 attccgccgc cgcctttgtg gacgacaacc ccaagctttg gcacaccgtc atctacgacc
     5881 ttgccgttta ccagcccgat gccatcgcct tcctcatcga acgctacggc gtggaaaaaa
     5941 tcctgctcgc cattcccagc gcgactcagg aacaacgccg ccgaatcatc agcaaactga
     6001 aagcctatcc gtgcgaagtg ttgaccattc ccggaatgaa agacctgatg gacgggaaaa
     6061 tcagcatcgg cacgctcaaa aaaatctctg tgtccgacct gctcgggcgc gattccgtcg
     6121 cacccgacga ccgcctcatg aatgccgaca ccgaaggcaa aaccgtcatg gtaaccggcg
     6181 cgggcggctc catcggttcg gaactctgcc gccagattat ccgccgccgc cccgaaaggc
     6241 tgctgctgtt cgagctgtcc gaattcgccc tgtacaccgt tgaaaaagaa ttgtgcgaat
     6301 actgcgcccg aaacggcatc gccgccgaaa tccttccctt tttgggttcg gtgcaaaacc
     6361 gcacgctgct cacgcacatt atgaccgcct tttccgttgc gaccgtctat cacgccgccg
     6421 cctacaaaca cgtccctatg gtcgagttca acaccgtcga gggcatacgc aacaacatct
     6481 tcggcacact cgaatgcgcg cttgccgcca cggcttcggg cgtggaaacc ttcgtcctca
     6541 tctccaccga caaagccgtc cgccccgcca acacgatggg tgcgggcaaa cgcatggcgg
     6601 aactctgcct tcaggcactc gccgccgaac ccggacaaaa aacccgcttc agcatggtac
     6661 gtttcggcaa tgttttaggc tcgtccggct ccgtcgtccc gctgtttgaa aaacagattg
     6721 ccgaaggcgg ccccatcacc ctgacccacc ccgacatcac gcgctatttc atgaccatac
     6781 ccgaagcggc gcaactggtt atccaagccg gcgcgatggg cagaggcggc gacgtgttcg
     6841 tcctcgatat gggcgaatcc gtcaaaatca ccgaccttgc ccgccaaatg attaccttaa
     6901 gcggactcaa acccaaaacg cccgaacagc ccgacggcga catcgaaatc ctcattaccg
     6961 ggctgcgccc cggcgaaaaa ctctacgaag agttgctcat cggcgacaac gtccgcaaaa
     7021 ccggccatcc gcgcatcatg accgccgacg agaccatgct gccgtggcac gaactctccg
     7081 ccctgctcga ccgcatccgc accgcctgcg accgttacga ccagcaggca atccgcgacc
     7141 tgctcgtcaa cgccccgacc ggctttaccc cgaccggcgg catctgcgac ctgctttggg
     7201 tatgcgaaac acacagaaaa aatgccgtct gaaccttcag acggcatacc gtacaaacca
     7261 atctgcctta cacacactga gtttgacatg cagttctcgg cattcggcga aaaattcacg
     7321 caacacagcg gcatcctgca actgatggac gacctcggcg acgcgctcaa aagcgacaag
     7381 cccgtcaata tgctcggcgg cggcaacccg gcgcgcattc ccgaaatcga tcgggcgttc
     7441 gccgacatat tttccaagct ggcggcggaa cacgccgtcg agaacatcgg caactactcc
     7501 aatccccaag gcgatgccgc gctgattgac gcgctgaccg ccttccccaa ccgcgaatac
     7561 ggctggaacc tgaccgtcgg caatatcgcg ctgaccaacg gttcgcaaaa cgcgtttttc
     7621 tatttattca acctcttcgg cggcaaattc aagctttcag acggcacatc cgcagaaaaa
     7681 gccattttgt tgccgctcgc gcccgaatac atcggctatg ccgacgtgca tgtcgaaggg
     7741 cggcacttcg tttccgtcaa acccaaaatc gaaaacgtcg aacacgaagg cgaagcaggc
     7801 ttcttcaaat accgcgtgga ctttgacgca ctggaaaacc tgcccgaact caaagcgggc
     7861 aaaatcggcg cgatttgctg ttcgcgcccg accaacccga ccggcaacgt gttgaccgac
     7921 ggcgaaatgg cgcgtttgga cgctttggcg cgtgaacacg gcattccgcc gattatcgac
     7981 aacgcctacg gaatgccgtt ccccaacatt attgacagcg gcgtaacgct gaattggcac
     8041 gaaaacatca tcctctgctt cagcctttcc aaagtcggcc tgccgggcgt tcgcaccggc
     8101 atcatcgtcg ccgcgcccga agccgtcaaa gccgtcagca gcctgaacgc gattgtgaac
     8161 cttgccccca cgcgcttcgg cgcggccatc gccgccccgc tgctggaaag cggcgagatg
     8221 aaacggcttg ccgaccaagt catccggccg ttttaccgca atcaggcgca aaccgccgtt
     8281 tcgctgctca agcgcgagct gggcgcgtac ccgatgaaaa tccacaaacc cgaaggcgcg
     8341 attttcctgt ggctctggtt tgaaaacctg cccgtttcgt cgcaaaccct gtacgaaatg
     8401 ctcaaagccg aaggtacgct gattattccg ggcgaacatt tcttcgtcgg catcgacacg
     8461 caagactacc cgcacgcggg cgagtgcatc cgtatgagta tcgcacagga cgctcaaacg
     8521 ctggaaaaag gcatcgccgc catcggcaaa accgtccgaa aaccgtacga caacgtttaa
     8581 aacgcaaaaa atgccgtctg aagacctttc agacggcatt ttcatccgca ttcaatgtcg
     8641 ggaaaaatgc gcccgcaccg gtttgcagtt ttccggaaga tcgggacacg cgccgaggat
     8701 gccgccgctg aagtcgtcta aacggtggaa gtcgctgccc gcgcttgcca acaatccgaa
     8761 gcggtctgcc aaaagcgcgt agttgaggcg gtcgtttttg cagcagttgc cgctgtggac
     8821 ttcgatgccc gcgccgccga ggtttttaaa ttcttcaaac aggttgcgct tggcggttgc
     8881 cgacaaatca taacgcatcg gatgggcaat caccgccatg ccgcccgcgc cgttgacggc
     8941 ggagacgcag tctgccagcg tcgcccattc gtggcgtacc gcgcaggatt tgccgtcgcc
     9001 caagtatttg gtgaacgcct gccgcttgtt tttgacgtgt ccgtttttga tgaggaactc
     9061 ggcgacgtgg gtgcggctga ccatttcttt gtttgccgcc agcgccagcg cgccgtcgta
     9121 tgcgccgccg atgcctttct tttcgagctt ggcggcgatg gcttcaagac gtttcagacg
     9181 gcctttgcgg acatctgcca acaggttttg caggttttcg tcctgctcgt cgaaatccaa
     9241 accgacaacg tgtatggtac gcccgcgcca cgttaccgaa atttccacgc cgttaatcag
     9301 gcgcaaaccg agcctgtcgg cttcggcacg cgcttcggag atgccgcccg tgtggtcgtg
     9361 gtcggtcaac gccagcagcg tgcagccgtt ttgatgtgcg aggcggacga cttcggcggg
     9421 ggggagcata ccgtcggaaa cggtggaatg gcagtgcagg tctatcatgg ggtgttatgt
     9481 ggtggtgaat gaaggacggg atgtttcaat gttgtcggac agtgtggcgg cggcggtcat
     9541 tttaaaaaac aggtgtatgg caaatgcaaa ggaaaagccg ctatgccgtc attcccgcgc
     9601 aggcgagaat ccagactttg atttattgga aatgtttaag gttaaccgct atttcaaact
     9661 tccggattcc tgctttcgag ggaatggcga tatgaacgtt ttcaatttta atctactata
     9721 aaagaccgtc tgaaaacgtg gttttatcaa gcccaaccgt tttcagacgg catcgtccgg
     9781 ccaacccgct tccttcagtt ttttccgttg cgcatcgtag cgtgccttgt ccgcccagta
     9841 aatcgtctga atgcacgcct cgccgcagtc gctgccgcag cattcccacg actcggggcg
     9901 gacgggttcg tccaaaagcg gctcgcccaa aagggcttcg gctttatgct tcagggtcgt
     9961 atccatcggc gatttccaag cgcgcgccgt caaactcgat gacttctccg ccgcgtattt
    10021 tggccgtttt gcggatttcg gttccgccgt tgcgcaacac cagcccttcg gcgataaatg
    10081 cttttgcctg tccgccgctt tcggcaagtc cggccaattt caagaggtcg cacaaagcga
    10141 tgtattcgtt gtcttcgaga tagacggtgg cttccataat gtcccctttt agaaagaggg
    10201 cgttattgta gcacctgccg ccgccgcacc caaaattgcc gcaaaaccgg cgatgtgtcc
    10261 gcactgcctg ttccgtaaaa atgccgtctg aaaccccgta tgccgccatc cgtccaaaga
    10321 aatcctgccc aacggcaggc tgcaaatcct gttccccgac gaatccgcat tgacgctgat
    10381 gcacatcctc aaacgcggac tgcccgatac accggcaatc ggcatcaaaa cgaaatcaaa
    10441 aacctgtttg aacgtaaaat ctatttaact gcttgccgtc cgtccgaata aggtatatag
    10501 ttccttataa ctagtttgat agtcctttat atctatcaat actccttggg aagcctccgc
    10561 cacacggcag gaggcatttt tttgccgtcg taaaagctca aaaaacattt gcaggtcatg
    10621 ccgtctgaat ccgaaacggc attacctgca ccgccatcta aagacaaccc tgctacaata
    10681 cgccttttat tgtccacgcc gattttgcca tgaccgagcc gacctacatc cccctgcgcc
    10741 tgcataccga attctccatt accgacggta tggtgcggat taaaaaactg attgccaaag
    10801 cgcaagaata cggtttgcct gctttgggca tcagcgattt gatgaacgaa ttcggtttgg
    10861 tgaaattcta taaagcctgc cgcggcgcag ggattaagcc cgtcggcgcg gcggacgtgt
    10921 ggataggcaa tccgaatgcg cccgacaagc cgttccgcgc catgctggtt atccgcaacg
    10981 atgcgggtta tctgcgcttg agcgagcttt tgacggaggc ttatgtcggc caagaccgca
    11041 atatccatca tgcggaactc aatcccgaat ggctggaaaa cggcgacaac agcggcttga
    11101 tttgtttgag cggcgcgcat tacggcgaag tgggcgtgaa tctgttgaac ggcaatgaag
    11161 acgcggcgcg tgcggcggcg ttgaaatatg cggcatggtt tcccgatacg ttttatttag
    11221 agctgcaacg cctgcccgaa cgccccgaat gggaggcttg cgtttccggc agcgtgaagc
    11281 tggcggagga actgggtttg ccggtggtag caacgcatcc gacacagttt atgaaccgcg
    11341 acgatttcaa cgcgcacgag gcgcgggtgt gcatcgcggg cggctgggtg ctgacggaca
    11401 agaaacgtcc gcgcgatttc acgccgagcc agtttttcat tccgccggaa acgatgctgg
    11461 agcgtttcgc cgacttgccc gaagccttgg aaaacacggt ggaaatcgcc aaacgctgca
    11521 acttgcacat cacattgggc aaaaacttcc tacccctttt ccccacgccc gacggtttat
    11581 cactcgatga ctgtctcatc aaactttcca acgagggttt gcaggaacgc atggttcagc
    11641 tttatcccga cgaggcggaa cgggcggcaa aaatgccgga atatcaggag cggctggact
    11701 ttgagctgaa catcatcatc caaatgaaat tccccggcta tttccttatc gtacaagact
    11761 ttatcaactg ggcgaaaacg cacggctgtc cggtcgggcc gggacgcggt tcgggcgcgg
    11821 gttcgctggt ggcgtattcg ctgaagatta ccgacctcga cccgctgaaa tacgcgctgc
    11881 tgttcgagcg tttcctaaac cccgaacgcg tttccatgcc cgactttgac gtggacttct
    11941 gtcaggcaaa ccgcggccgc gtgattgaat acgtgcgcga aaaatacggc gctcaggcgg
    12001 tcagccagat tgtgactttc ggcacgatgt cgtccaaagc ggtcatccgc gacgtggggc
    12061 gtgtgctgga actgccgttt atgctgtgcg acaaactgtc caagctgatt ccgttggaag
    12121 ccaacaaacc cctgagtttg gacgacgcga tgaaggcgca gccgcagatt caggaattgc
    12181 ttgaagcgga agaagcggac gaactgatta cgctggcgaa aaaactggaa gacctgacgc
    12241 gcggtttggg tatgcacgca ggcggcgtgt tgattgcgcc gggcaagatt tccgattaca
    12301 gccccgtgta tcaggcggac gaatccgcct cgcccgtatc catgtacgac aagggcgacg
    12361 tggaagacgt gggtttggtg aagttcgact ttttgggcct gcgcaacctg accattatcg
    12421 aaatggcgca gaacaacatc aaaaacaccg ccggcgatat cgtcgatgtc ggcaagattc
    12481 cgcttgacga ccagactgcc taccaaatct tccgcgatgc gaacaccacc gccgtcttcc
    12541 agttcgagtc gaccggcatg aaaaaaatgc tgaaaacggc gcatacgacc aagtttgaag
    12601 aactcatcgc cttcgtatcg ctttaccgac ccggcccgat ggacaacatt cccgacttcg
    12661 tcgcacgcat gaagggacag gaattccaat acatccaccc gctgctggaa ggcatcctcg
    12721 cgccgaccta cggcattatg gtgtatcagg aacaagtgat gcaggcggcg cagattatcg
    12781 gcggctactc gctcggcggc gcggacctgc tgcgccgcgc catgggtaag aaaaagcccg
    12841 aagagatggt gaaacaccgc gaaatcttcg ccgaaggtgc ggcaaaacaa ggcatttcgc
    12901 gcgaaaaatc cgacgaaatc ttcaactaca tggaaaaatt cgccggctac ggtttcaaca
    12961 aatcccacgc cgccgcctac gccctgattt cctaccagac cgcatggctc aaagcccact
    13021 atcccgccga atttatggcg gcgacgatgt cgtccgaatt ggacaacacc gaccagctca
    13081 aacatttcta cgacgactgc cgcgccaacg gcatcgagtt cctgccgccc gacattaacg
    13141 aatccgacta ccgcttcacg ccgtatccgg acatgaaaat ccgctacgca ctcggcgcga
    13201 ttaaaggcac gggcgaagcc gccgtcgaat ccatcatcgc cgcgcggcaa agcggcggca
    13261 aatttaccgg cctgttggac ttctgcgagc gcgtcggcaa agaacacatg aaccgccgca
    13321 ccctcgaagc cctgatacgc ggcggcgcgt tcgacagcat cgaacccaac cgcgccatgc
    13381 tcttggcgaa catcgacctt gccatggaca acgccgacca aaaagccgcc aacgccaatc
    13441 agggcgggct gtttgacatg atggaagacg ccatcgaacc ggtgcggctc atcgacgcac
    13501 ccatgtggag cgaatcggaa aaactcgccg aagaaaaaac cgtcatcggc ttttacctgt
    13561 ccggccaccc gttcggcccg tatgcccaag aagtccgcca aatcgccccg accaaattag
    13621 gccgtctgaa accgcaagac agcgtgcgcc tcgccggatt cgttaccgcc gtgcgcacca
    13681 tgatgggcaa acgcggcaaa atcgccttcg tcagcctcga agattcgagc gggcaggctg
    13741 aaatcatggt cggcggacaa acgctggaaa actgcgccga ctgcctcaaa gccgaccaag
    13801 tgctgattat cgaatccaaa gtcagccgcg acgattacgg cggcggcgac gggctgcgca
    13861 tcatggcaaa ccaagtcatg accctgcaaa cggcgcgcga acgctacgcc cgcagcctca
    13921 gcctcgccct tgccccgcat cacgacatcg gcggactggt acggctgctc gccgcccacc
    13981 aactgcccga cacgccgcgc atcccgcttc aattgtcgta tgccaacgaa aaagcgtcgg
    14041 gcaggcttca agtgcctccg aaatggacgg ttacaccgag ttccgcattg ttcggcgaac
    14101 tggaaacatt gctcggcagc cggtcggtgc gcgtgaattg gtaaccaaaa atacaaatgc
    14161 cgtctgaagc ccaaaaccgg tttcattcgt actttattcg aatgattgaa taaaagtaac
    14221 tgccaagaaa aacgtatttt ttggttattt cgccagccta aataaatcaa ccggggcgat
    14281 tgatatccgt gtacatgaca cagacagcac caaagggaaa aacggcattt tcaaaagtat
    14341 cggtgtcaaa accgcccttt cattccaaaa ataccaaatc gacgaaccgg gcaaagaaat
    14401 cagaccgtgc cgcctgaaaa aacgcccgcc cgtccgttaa attaatctat ccctgtttca
    14461 gacggcctga aacagggatt tttatatcaa gataaaatga gaacgggagc gacaaccacg
    14521 ggcaaagtcg gcgcgaacta tcaaaaattg tagcactgcg aaatgagaac gggagcaata
    14581 acccttaggt agctcttgtt atagcggatt aacgaaaatc aggacaaggc gacgaagccg
    14641 cagacagtac aaatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt
    14701 tctctttgag ctaaggcggg gcaacgccgt accggttttt gttaatccgc tatattttcc
    14761 gatgcaaaac aaagcagtca tatatttgat tccccctacc gttgccacgc ttccctcaaa
    14821 tattcgacgc agtcggtcag cgagtaaaac gggacattgc cgtggtcggc attcggatat
    14881 tcccggaaaa atacggcggt cccgcgcctg tccaactctg ccgccatttg ttcggcctgc
    14941 cctgccatgt cgcgttcttc cctgcgtttg caaccgctgc tccgctcgag tgcgccgatg
    15001 ttgaggcagg catcgatgcc gtccagccgg ttttcagacg gcataaagtc gagtatccgc
    15061 ctgttgtgcc accaaatcga gggggatacg agccaatgcc gtctgaaaca gcggtgggaa
    15121 agcagggaat agagtccgaa cagtgcgccg aacgagtgtc cgaatacggc ggtttcatta
    15181 cggttaaggg tgtagcggct ttctaaaaag gcggtcagtt cgctgtcgat aaagtcggcg
    15241 aagcggtctg cctgtccgaa ctgccgccgt tcgtctgctg tggcgttgtc tccaagcggc
    15301 ggcgtgtagt cggcggcacg ctgcgccagg tcgcgcacac tgcctgtcgt gtagccgata
    15361 ccgacaatca ggcagggggc gttgcttcgg gtaacggggt tgttcatcag cgactgcatg
    15421 atgttgtaca gcgcgggaaa aaggcttcgc cgtcgaggac aaagaggacg ggatagcctt
    15481 cagactgcat ttcgccgagt gttgccgtct gaatccgata gattcgcccc gtgcaggtgg
    15541 atttgatttc ggtttcaaag gcttggggca gtatggcagg ttggaatgtg tcggtcggta
    15601 tgggtttcat gatgttcggc ttgtgggtca gactggcagc aagcggtaaa accgaaccgg
    15661 caaagcggtc tgcctgccgg ttcggtctgt tttccttcgc aatgccatac tccggttgtg
    15721 agcgcatagg gtatgccgcg cagcttgttg tggtttgatt atgctgcggc tgccgtttca
    15781 gacggcatat tggtctttaa aaactgtaac gcaggtttgc cgtcaggctg cgctccgaac
    15841 cgggaatgtt aaaggtgttc gtgccgccga cgcgggcgta gtaatggcgg ttgaagatgt
    15901 tgtcggcatt gatttgcagc ttcagcttgg gcgtgaagcg gtatgccgcc atcgcatcga
    15961 acgtggcata accgcctgca tgcatccctg cagatgaagt aatgccgctc atcgcgttca
    16021 cgccgccgcc gacggtcagc ccgggcgtaa cttggtaagt cgtccacagg tttgcgctgt
    16081 gtttgggcac cagcaggaag atgccgtcgt cgcgtggatt ggcggcggtt ttgatttggc
    16141 tgtgcagata gctgtaacct gcatggattt gccatttcgg tgtaatcgca ccgctgattt
    16201 cggtctcaac gccttccatc acgcgtttgc ccaatgcggc gtaacgggtg ttgcggttgt
    16261 tcgggttcgg cggcgttttt gtctttcagg cggcaggggg agacgcgggt attgaggcgg
    16321 tcatccatat agctgccttt aacgagctgt aatcgccgtt tttctcggtg gcaatcgcgt
    16381 tgtacgaacg ctgccctttg atatggacgg tttccaaatc cgccgtttgt gcggcaaaaa
    16441 ccggagacga gagtgctgcc aaaaccgtgg cggcggtcat attgatgcgg aaaactgaca
    16501 taaactgtcc catctgtata aatgataatg gttctatttt aataaagcac aacgcggctt
    16561 gttcggaaaa acatatcgct cagccgacaa attttgtcga aaatgcgaca cgtctgcgtt
    16621 ttccgcataa aatctgcttt tttactgcaa ccaacctgcc atgaccccgc ccaaactcat
    16681 catcttcgac tgggacggca cgcttgccga tacgacccaa cccatcatcg acaccatgcg
    16741 ccgcagcttc gccgaatgcg gttttccgcc gcccgaagcg gagcgcgtcc gcagcctgat
    16801 cggatacagc ctgcccgaaa tcatccgcgc cctgctcgaa atgccttctg aagctgccgt
    16861 tgccgacatc gcgcgcactt attccgcgca ttacctcaat cccaacaacc gcaatatgac
    16921 actctttccc gatgccccgc cctgtctgga caagctcaaa gcgcaaggct tccggcttgc
    16981 cgtcgccacg ggcaaagggc gggcgggttt ggacaacgcc atcagccaaa ccgccaccgg
    17041 cggctattgg cttgccaccg cctgcgcggg ggaatacccc tccaaaccct cgcccgaaat
    17101 ggtattcgga ctctgcggcg aactgggact cgacccgaaa gaggcattgg ttgtcggcga
    17161 tacggcgcac gacctgcata tggcggcaaa cgcaggcgcg gcggcagtcg gcgttgccac
    17221 cggcgcacat tcgcgcgaac agctccttgg cgcaccgcat ctcgccgtat tggacggttt
    17281 gtccgaactg cccggttttc ttgcacgaca ttacgcctga ttggtttccg catccggcac
    17341 gcggcaaaaa tgccgtctga agcctgttca gacggcatga ggcctttgca aaaaagccct
    17401 tccttcg
//
LOCUS       DAXFZR010000049        17065 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000049 DAXFZR010000000
VERSION     DAXFZR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17065
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.049"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(81..419)
                     /locus_tag="ACFJAS_001378"
     CDS             complement(81..419)
                     /locus_tag="ACFJAS_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC3982610.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            570..4526
                     /gene="purL"
                     /locus_tag="ACFJAS_001379"
     CDS             570..4526
                     /gene="purL"
                     /locus_tag="ACFJAS_001379"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC3982611.1"
                     /translation="MSVVLPLRGVTALSDFRIEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            complement(4597..5808)
                     /locus_tag="ACFJAS_001380"
     CDS             complement(4597..5808)
                     /locus_tag="ACFJAS_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFC3982612.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARTQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5882..6193)
                     /locus_tag="ACFJAS_001381"
     CDS             complement(5882..6193)
                     /locus_tag="ACFJAS_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC3982613.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            6425..7177
                     /gene="gloB"
                     /locus_tag="ACFJAS_001382"
     CDS             6425..7177
                     /gene="gloB"
                     /locus_tag="ACFJAS_001382"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC3982614.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            7411..8865
                     /gene="mgtE"
                     /locus_tag="ACFJAS_001383"
     CDS             7411..8865
                     /gene="mgtE"
                     /locus_tag="ACFJAS_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC3982615.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(8951..9859)
                     /gene="hslO"
                     /locus_tag="ACFJAS_001384"
     CDS             complement(8951..9859)
                     /gene="hslO"
                     /locus_tag="ACFJAS_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC3982616.1"
                     /translation="MNQTAINCADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            10104..10772
                     /locus_tag="ACFJAS_001385"
     CDS             10104..10772
                     /locus_tag="ACFJAS_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC3982617.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPVNRAPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNADELIGSAMGLL
                     GIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMV
                     FFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARRIKKNDPS
                     RFLN"
     gene            10791..11009
                     /locus_tag="ACFJAS_001386"
     CDS             10791..11009
                     /locus_tag="ACFJAS_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982618.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            11154..11498
                     /locus_tag="ACFJAS_001387"
     CDS             11154..11498
                     /locus_tag="ACFJAS_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC3982619.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            11498..12190
                     /locus_tag="ACFJAS_001388"
     CDS             11498..12190
                     /locus_tag="ACFJAS_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC3982620.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITSSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            12257..13477
                     /gene="argJ"
                     /locus_tag="ACFJAS_001389"
     CDS             12257..13477
                     /gene="argJ"
                     /locus_tag="ACFJAS_001389"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC3982621.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            13537..14946
                     /locus_tag="ACFJAS_001390"
     CDS             13537..14946
                     /locus_tag="ACFJAS_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC3982622.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRRRVLQETAAQTG
                     NAPARPQAANSKTGMPSEN"
     gene            15134..16525
                     /gene="hrpA"
                     /locus_tag="ACFJAS_001391"
     CDS             15134..16525
                     /gene="hrpA"
                     /locus_tag="ACFJAS_001391"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC3982623.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDDTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEEN"
BASE COUNT         3867 a         5119 c         4410 g         3669 t
ORIGIN      
        1 aacatatctg ctaatctggt acagacccaa aaacaaatgc cgtctgaaag cctttcagac
       61 ggcatttcca ttccaaacgc tcaaaccgcc gcgtccgaac gttcgcccgt gcggatacgg
      121 attgcctcct cgaccggcag cacaaaaatc ttgccgtcgc cgattttgcc cgaacgcgcc
      181 acctcgacaa tcacgtcaat cgcgcgttcc acggcatcat ccgccaacac caactcgatt
      241 ttgaccttgg gcaggaaatc gacggcgtat tccgcaccgc gatagatttc cgtatgcccc
      301 ttctgcctgc cgaacccttt gacctcgctg acggtcatgc ccgtaatgcc gatttccgtc
      361 aacgcctcgc gcacgtcgtc gagcttgaac ggtttgacaa tcgcctcgat ttttttcata
      421 aaatttcctt tgaacaaaca atacaaacac atccgaaaaa cgggaacccc cgtcagattg
      481 tcaacatttt aaaccaaaat acccaagcaa tacagccccc gttgcgcata taatgacagc
      541 ttttccaacc gcatttgaga gccgaatcca tgtctgttgt tttgcccttg cgcggcgtta
      601 ccgccctttc cgatttccgt atcgaaaaac tcttgcaaaa agccgccgca ctcggcctgc
      661 ccgaagtcaa attaagcagc gaattttggt atttcgccgg cagcgagaaa gcacttgatg
      721 ccgcgactgt cgaaaaactg caagccttgt tggcggcgca aagcgttgaa caaacgcccg
      781 aagcgcgcga gggtttgcat ttgtttttgg taacgccccg tttgggtacg atttcgccgt
      841 gggcttccaa ggcgaccaat atcgcggaaa actgcggttt ggcaggcatc gaacgcatcg
      901 agcgcggtat ggcggtgtgg ctggaaggtg cgcttaccga tgaacagaaa cagcaatggg
      961 cggcgttgct gcacgaccgc atgaccgaat ccgtgctgcc cgattttcag acggcatcca
     1021 aattattcca ccatctcaaa tccgaaacct tctccaccgt cgatgttttg ggcggcggta
     1081 aagaggcttt ggtcaaagcc aataccgaaa cgggtttggc actctccgcc gacgaaatcg
     1141 attatctggt tgaaaactat caggctttgc agcgcaatcc gtccgatgtg gagctgatga
     1201 tgttcgcgca ggcaaacagc gaacactgcc gccacaaaat cttcaacgcc gatttcatcc
     1261 tcaacggcga aaaacaaccg aaatccctgt tcggcatgat acgcgacacg cacaacgcgc
     1321 atcccgaagg tacggtcgtc gcctataaag acaattcgtc cgtgatcaaa ggcgcgaaaa
     1381 tcgagcgttt ctatccgaat gcagcggaaa accaaggcta ccgtttccac gaggaagaca
     1441 cccacatcat catgaaagtg gaaacgcaca accacccgac cgccatcgcg ccgtttgcgg
     1501 gcgcggcaac aggcgcgggc ggcgaaatcc gcgacgaagg tgcgacgggc aaaggttcgc
     1561 gtccgaaagc gggcttgacc ggctttaccg tgtccaacct caacatcccc gacctcaaac
     1621 agccgtggga acaagactac ggcaagccgg aacacatttc ctcgccgttg gacatcatga
     1681 ttgaaggccc gattggcggt gcggcgttca acaacgaatt cggccgcccg aacctcttgg
     1741 gctacttccg cacctttgaa gaaaaatttg acggtcaggt tcgcggctac cacaaaccga
     1801 ttatgattgc cggcggtttg ggcagcattc aggcgcagca gacgcacaaa gacgaaatcc
     1861 ccgaaggcgc actgctgatc caactgggcg gcccgggcat gctcatcggc ttgggcggcg
     1921 gtgcggcttc ttcgatggat accggtacaa atgatgcgtc tttggacttc aactccgtac
     1981 aacgcggcaa ccctgaaatc gaacgccgcg cgcaggaagt catcgaccgc tgctggcagc
     2041 tcggcgacca aaacccgatt atctccatcc acgacgtggg cgcgggcggc ttgtccaacg
     2101 ctttccctga attggtcaac gatgccggac gcggcgcggt attcgagctg cgcgaagtgc
     2161 cgttggaaga acacggttta acgcctttgc aaatctggtg taacgaatcg caagagcgtt
     2221 atgtcttgtc gattttggaa aaagatttgg acaccttccg cgccatctgc gaacgcgaac
     2281 gctgcccgtt tgccgtagtc ggcacggcga ccgacgacgg tcatttgaaa gtgcgcgacg
     2341 atttgttctc caacaacccc gtcgatttgc cgctgaacgt cttgctcggc aaaccgccca
     2401 aaaccacgcg taccgacaaa acggttacgc cgtccaaaaa accgtttcac gcgggcgata
     2461 tcgacatcac cgaagccgcc taccgcgttt tgcgcctgcc tgccgtagcc gccaaaaact
     2521 tcctgattac catcggcgac cgcagcgtcg gcggcatgac ccaccgcgac caaatggtcg
     2581 gcaaatacca aacccccgta gccgactgcg ccgttaccat gatgggcttc aacacctatc
     2641 gcggcgaagc gatgtctatg ggcgaaaaac cggccgtcgc cctgtttgac gcgcccgcct
     2701 cgggcagaat gtgcgtcggc gaagccatca ccaacatcgc ggcagtcaac atcggcgaca
     2761 tcggcaacat caaactttcc gccaactgga tggcggcgtg cggcaacgaa ggcgaagacg
     2821 aaaaactcta ccgcaccgtc gaagccgttt ctaaagcctg ccaggcattg gatttgagca
     2881 ttcccgtagg taaagacagc ctgtcgatga aaaccgtgtg gcaggacggc gaagagaaaa
     2941 aatccgtggt ttcgccgttg agcctgatta tttccgcgtt cgcgcctgtt aaagacgtgc
     3001 gaaaaaccgt tacgcccgaa ttgaaaaacg tcgaaggcag cgtattgctg tttatcgatt
     3061 tgggcttcgg caaagcgcgc atgggcggct cggcgttcgg tcaggtgtac aacaacatga
     3121 gcggcgacgc gcccgatttg gacgatgcag gccgtctgaa agcgttttac agcgtaattc
     3181 aacagcttgt cgccgaagac aaacttttgg cgtatcacga ccgcagcgac ggcggtttgt
     3241 ttgcgacgct ggcggaaatg gcgtttgcgg cacggtgcgg tattagtgcc gatatagatt
     3301 gcctgatgga taaattcctg ccgattcatc tcccggattt tcaaggcgac cctgccgaag
     3361 acttatctga cgaactttat aatcatgccg ccattaaaat cttattcaat gaagaattag
     3421 gcgcggttat ccaaatccgc caaaaagata gggattatgt tgatgcggca tttgaaacgg
     3481 ccggcttaac cgatgcggtc agccggattg gctctcctga ttttgacaat gagtttattt
     3541 ctttctttgg ttacggttat ttcttggaac aaaaccgcgc cgacctgcaa cgcgcgtggc
     3601 aggaaaccag ccacgccatc caacgcctgc gcgacaaccc tgcctgcgcc gacagcgagt
     3661 tcgccctgat tggcgacaac gaacgcagcg cattgtttgc cgacgtgaag ttcgacgtga
     3721 acgaagacat cgccgcgccg tttatcaaca gtggcgcgaa acctaaaatc gccatcctgc
     3781 gcgaacaggg cgtaaacggg caaatcgaaa tggccgccgc cttcacccgc gccggattcg
     3841 atgcctacga cgtgcatatg tccgacctga tggctggccg cttccgcctt gccgacttca
     3901 aaatgctggc ggcgtgcggc ggcttcagct acggcgacgt actcggcgcg ggtgaaggct
     3961 gggcgaaatc catcctgttc caccctgcct tgcgcgacca attcgccgcc ttctttgccg
     4021 atccgaacac gctgacattg ggcgtgtgca acggctgcca aatggtcagc aaccttgccg
     4081 aaattatccc gggcgcagaa acatggccga agttcaaacg caacctgagc gagcagtttg
     4141 aagcgcgcct gaatatggtt cacgtcccca aatcagcgtc gctgattttg aacgaaatgc
     4201 aagactccag cctgcctgtc gtggtcagcc acggcgaagg ccgcgccgac ttcgcgcttc
     4261 acggcggcaa tatttccgcc gatttgggca tcgcgctgca atatgtggac ggtcaaaacc
     4321 aggttaccca aacctatccg ctcaacccca acggctcgcc gcagggcatc gccggcatta
     4381 ccaacgccga cggccgcgtt accattatga tgccccaccc cgaacgcgtg taccgtgccg
     4441 cacaaatgag ccggaaaccg gaaggctgga cggaactgtc cggctggtac cgcctctttg
     4501 ccggcgcacg taaagccttg ggctaaaccg tctgcccaaa caaatgccgt ctgaaaaagg
     4561 ttttgaaacc cgtttcagac ggtattttga ccattctcaa gccctgccgg tactcaatcc
     4621 cgctttaccg ttttcccgca aagttttcag caatactttc cacgccgaca atttagggtt
     4681 ggactgtttt ccttgtccga tcaaatccat ttgcgtcata taccagttca tttctaacat
     4741 tgcacccgct tttttatcaa caaatgctac gccagtttgg atgcggtctg cttgtacggt
     4801 ttggtaagtt ctgttttgca ttttgttcgc caaatcaggg actaaggcaa acgggcgggc
     4861 tatgccgacc aaatccaaat gaccgctgga taaggcatct tccatggcgg tttgcgaacg
     4921 gaatccgccg gtgataatca gcggggcttg gctggctgca cgggcttttt cggcgtaatc
     4981 gatgaagaag gcttcgcgtt tgcgggtgct gtctttggcg gcgagcattt gcgggctttc
     5041 gtagttgcca ccggaaactt caataaaatc aatgcccatt tccgacagtt tttgcaccac
     5101 ctgcaccgat tcgctttcgt caaatccgcc tttttggaaa tctgccgaat tgagttttac
     5161 gcccaccaag aaatctttgc ccgcggcagc gcgaatagcg gtgtaggttt ccaaaaggaa
     5221 gcgcatacgg ttttccaaac tgccgcccca ttggtcttgg cggcggttgt ggtgcggcga
     5281 gaggaattgg ctgatgagat aaccgtgtac ggcgtaaatt tgtacgcctg aaaagcctgc
     5341 ctgttcggca attttggcag tttgtacgaa ttgctgaatc agcccgttga tttcatcggc
     5401 actcaattcg cgcggcggat tgataaagcc attcatgccc accagcggca cggcgctcgg
     5461 tgcaagcggt gttttattga ccaccgcagg cgactgtttg cccgcatgat tgatttgcat
     5521 gatgagcagc gtgtcgtttt gcgtgcgggc tttcgcccat tttttcagca tttcaagggc
     5581 gcggtcgtct gaaatcaaca catcgtttat cgaacctttg ccgctttcag ccaccatcac
     5641 attgcccgtc accaaaacgc ctgcgccgcc ttctgcccaa gcgccgtaca ggcggacaag
     5701 tttttcagac ggctggtcgt tttgggcaag ttgctcttcc atggcggatt tgaagatacg
     5761 gtttttggcg gttttgccgt tttgaaaagt gaatggcgtg aataacatga tcgttccttt
     5821 aagttgataa gtttaaaaat gtaaacgtat ttgggtaaaa ataaagggcc aacggataac
     5881 cctattttga aagcgggcaa ttttgagcca tcaaatcggc aagctgtttc aatctcgcca
     5941 ccgcatccgc catggcaata ctgtaaaacc gctctttgcc gacctgctcc accgccaccg
     6001 ctccggcttg cagcatgatt tttaaatggt gcgacacagc agggcgcgac agatgcagat
     6061 gctcggtcag ctcattcaca ttcatcctac cgtgtttcca caatacatgc aggatctgat
     6121 ggcggttttc atcgctcaat acggtaaaaa tgggtatgca ctcgcgcatt aaattcatgg
     6181 tttgttgcgg catattcgat ctattgagtt tatatattta aaaattcgaa cgtattttag
     6241 agggaaggtc ggcagttatc aaaggcagtt tggtttgcag tttggcaata tattgttgct
     6301 gaaaggtttt tataagaaaa aatgccgcct gaaacagcat gatggcgttt gctagggttc
     6361 cgctgtatat taacgccttt cggatacacc tttcagacgg catccgaacc atcaaggaac
     6421 actcatgaaa attacccccg tcaaagccct gaccgacaac tacatctgga tgatacagca
     6481 cggcaaccat gccgtctgcg tcgatccttc cgaaccctcg cccgtcttgg aattcctcgt
     6541 acgcaaccga ctcatgctcg cccaaacctg ggtaacgcac ccccatcccg accacgaagg
     6601 cggtgcggcg gcactctggc gcggctacat ggaatcgccc gtttacggcg aatccgacat
     6661 cgaagccgcc acccacaccg taaccgccgg cacacgattc accttcggca acggtcaggt
     6721 taccgtttgg gcaacacccg gccacacaga ccgccacacc agctaccttc tcgaaacttc
     6781 agacggcata cacgttttct gcggcgacac ccttttttcc gccggctgcg gacgcgtgtt
     6841 taccggcacg gtcgaacagc tttacgacag cttccaacgg ttcaaccaat tacccgaagg
     6901 caccctgttc tatccggcac acgaatacac cgccgccaac ctgcgtttcg ccgcccatat
     6961 cgagccggac aacgccgaca ttcagacggc attgaaggcg gcggaacgca cgcccaccct
     7021 gcccgttacc ctcgcgcacg aacgccgcgt caacccgttc ttacggacag aaatccccgc
     7081 cgtccgccaa cgtgccgaag ccctggtcgg caaaacgctg aacagcggtt tggaagtatt
     7141 cgccgccctg cgcgaactga aaaacgccta ccgctgacct gccctccgaa aaatgccgtc
     7201 tgaaacccgc gtttcagacg gcatttgcgt taaaaatagt aaaccgtttc aaaagggagt
     7261 agaatagtgc tgtttccaat cccgtgcctg atttttcggg cttttattat ggaccttccc
     7321 agttcgtttt tactgaacac cccttccgat tccaaacgac aataaccatc ccgccggaat
     7381 gcctccccgc acacggcggg cggagcattt atgagcatcg aacacactcc tccgacacac
     7441 gacggcgaaa ccggtcaaaa ccatgccgaa cgcccttccg ccgatttcga ccgtgtccac
     7501 tccctctgcg aaatcctcga acctgctttt gaacaaatcg aaaacggcac accgctcgaa
     7561 gatgcgccgc tgcgcgacaa gctgaccgag ctgaccgtcc tcttggccga gttgcaccct
     7621 gccgacgtgg ccgacgtctt ggagtccctg ccgccgcgcg aacgcaatat cgtttggctt
     7681 ctggtcaaac ccgaagacga cggcgaagta ttgctggaag tatccgacgc ggtgcgagaa
     7741 acgctgatcg agtcaatgga caaggacgaa ttgttggcgg cggtcgatga tttggatgcg
     7801 gacgaattgg cggagctggc agacgatttg ccgcaccaag tggtttatga agccttacag
     7861 acgcgcgatg aggaagagcg cgcccaagtc aaggcggcaa tgtcgtatga agacaaccaa
     7921 gtcggtgcga ttatggactt cgagttggtc agcatccgcg ccgatgtcgc ctgcgaagtg
     7981 gtgctgcgtt atctgcgccg cttcgacagc ctgcccgacc ataccgacaa gatttttgtc
     8041 gtcgatgaaa acgacgtgct gcagggcgtg ctgcccatcc gcaaactttt ggtcgccgat
     8101 cccgaagatt tggtggaaaa cgtgatggcg aaagatgtcg tgcgtttccg cgccgaggac
     8161 gacgtggaag aagcggcgca ggcgtttgaa cgctacgact tggttaccgc gcccgtcgtt
     8221 gatgaaaaca aaaagctcat cggcaggatt accatcgacg agatggtgga cgtgatccgc
     8281 gaagaatccg aagcggatat gttgaacatg gcaggtttgc aggaagagga agacctgttc
     8341 gcccccgtct tggattccgt caaaaaccgc tggatgtggc tcgccgtcaa cctctgcacc
     8401 gccttcctcg ccagccgcgt catcggcgcg tttgaaggca gtatcgaaaa aatcgtcgca
     8461 ctcgccgcgc tgatgcccat cgtcgccggc atcggcggta actcgggcaa ccagacgatt
     8521 accatgattg tccgcgcgat ggcgatgggg cagctgacgg atatgcaggc gggaaggctg
     8581 ttgaaaaaag aagtcggcgt cgccttggtc aacggcatta tttgggggac ggtgatggga
     8641 gcggtttcgt ggctgcttta cggcagcctc ggcatcggtt tggtcatggt tgccgcgatg
     8701 acgctcaacc tcctgctggc ggcaacggtc ggcgtattga ttcccgtggt aatggaaaaa
     8761 ttcggacgcg atcccgcgct gggcagctcg gtgctgatta ccgccgttac cgactccggc
     8821 ggcttcctca ttttcttgag tctcgccacc ctgttcctgc tttaaatgcc gtcccgaccc
     8881 gcgcaaaaat gccgtctgaa gcagaagctg cttcagacgg cattcgacca ttcatccttg
     8941 ttgctcaaga ttattggacg gtatgtcggg gcagcccttt ggcaacgccg accacatcct
     9001 ccccgaacag cgcgttgaca tcggtttcat caaacacgta tttgctgtgg cagaagtcgc
     9061 aatcgacttc gatgctgcct tgttccgcca ccacgccgcc gacttcttcc ccgcccagca
     9121 tcaacagcat atcgctgacc ttgccccgag aacaggtaca tgaggattca aacgtttccg
     9181 gctcgaacac gcgcggcggc gtttcgtgga acaggcggta taaaacgtgt tgcgcgtcca
     9241 attctgccag ctcctccgcc gtcagcgtgc gcgccagcgt actgacgtgt tcccatgcct
     9301 cttcatccaa cacctcttca ggcagacgct gcaccagcag cccgcccgcc gcttcatcgc
     9361 ttgcagacag ggcgatgtgc gtatcaagct gttcggaacg tttcatatag ttcaccaaca
     9421 tttgcgcgat gctgccgcct tccaaaggca ctacgccctg ccagggttcg ccgtctttgg
     9481 gttgcagcgt caatacgaac acgccgttgc cgcccaaaag gtcgccgagg ctttcgtcat
     9541 cggctatttc tgcggtttcg tcccaccgcg cggttgcacg gacggtacgg tcggaagtcg
     9601 cttccgcaac cagcattttc agttttccct gcccctgaac ctgcacaatc agcgtgcctt
     9661 cgtttttaag gttgcccgac agcagaacgc cggcagccag tagctctccc aaggcgcagc
     9721 ggatggcggc gggatagttt ttctgtttta caatgtgctg ccacacgttt tccagacgga
     9781 cgtgcagccc gcgcacgggc atatcgtcga agataaagcg ggtacgcgca tcggcgcagt
     9841 tgatggcggt ttgattcatg attttctctg actgattgtt cggatggcgg ctatatggtt
     9901 gcggtcggcg cgaaaacaag gcggacggcg gatgcgcttc ccaaattctc aataaattat
     9961 ataaaaatca acatattaac tcaatctaac aagccgtttt ttgccaaaca gccgtttttt
    10021 tatatacaat caacaagata ttttcgactg atacagcata acatcgcacg gcggcacgat
    10081 gcctcctgcg cggaaacacc gatatggatt cttttttcaa accggcagtt tgggcggttt
    10141 tgtggctgat gtttgccgtc cgccccgccc ttgccgacga gttgaccaac ctgctcagca
    10201 gccgcgagca gattctcaga cagtttgccg aagacgaaca gcccgtttta cccgtcaacc
    10261 gagcccccgc ccggcgggcg ggcaatgccg acgaactcat cggcaacgcg atggggctta
    10321 acgaacagcc cgttttaccc gtcaaccgag cccccgcccg gcgggcgggc aatgccgacg
    10381 aactcatcgg cagcgcgatg gggcttttgg gtattgccta ccgctacggc ggcacatcgg
    10441 tttctaccgg ttttgactgc agcggcttca tgcagcacat cttcaaacgc gccatgggca
    10501 tcaacctgcc gcgcacgtcg gcggaacagg cgcggatggg tacgccggtt gcccgaagcg
    10561 aattgcagcc cggggatatg gtgtttttcc gcacgctcgg cggcagccgc atttcccatg
    10621 tcggacttta tatcggcaac aaccgcttca tccacgcgcc gcgcacgggg aaaaatatcg
    10681 aaatcaccag cctgagccac aaatattgga gcggcaaata tgcgttcgcc cgccggatca
    10741 agaaaaacga cccgtcacgc tttctgaact gatttcccaa ggaatacgca atgagtatgc
    10801 ccgaaatgcc caaatggtac ggcgatgacg gacagatcgt atcctgtacc gaaaaggtca
    10861 aagtgatgtc cgaaaatatg gccgagctgt atcagacggc acaagacgcg tttgaagacg
    10921 cgctgctgat gggttgcggc gaacggcagt tgcgcgctta cctgctcgcg ctgattgaag
    10981 gtttggaaaa tccctaccgc aaagtctgaa cacgccccgg ttgctgcggc acggtttatc
    11041 cgtgccgttt ttgcgtttgt gcgcggcttc ggcttttcag acggcatatt tgacgttatg
    11101 attaaacagt taacaagatt tatcacaacg ccgtcaaaag acagacacac aacatgaaca
    11161 tcatccgcgc gctcctcatc atcctcggct gcctcgccgc cggcgaaacc gccgttttcc
    11221 tagcaggcat caaactgccc ggcagcatcg tcggcatggg cgtgctgttt gcgcttttgc
    11281 aggcgggttg ggtcaaaacg tcttggctgc aacagcttac cgacgcgctg atggcaaacc
    11341 tgacgctgtt cctcgtgccg ccctgcgtgg cggtcatcag ctatttggat ttgattgccg
    11401 acgattggtt ttcgatactg gtttccgcct ccgccagcac tttgtgcgta ctgctggtta
    11461 cgggcaaggt tcaccgctgg atacggagca ttatctgatg agcgaaatcc tcaggcagcc
    11521 cggcatcctg cttttcctca cgcttgccgt gtacgcgctt gcgattatcg tgcgcacgcg
    11581 cacgggcaat atcttctgca accccgtact cgtcagcact atcgtgctga ttgcctacct
    11641 gaaaatcctc ggtatcgatt atgcggtgta ccacaacgcc gcgcaattca ttgattttcg
    11701 gctgaaaccc gccgtcgtcg tgcttgccgt gccgctctac caaaaccgcc gtaaaatctt
    11761 caaccagtgg ctgcccgtca tcgtttcgca gcttgcgggc agcgttacgg gcattgttac
    11821 ggggatgtat tttgccaagt ggctgggcgc ggaacgcgaa gtcgtcctcc cgctcgcgtc
    11881 caaatctgtt accaacccca tcgccatcga aatcaccagc tccatcggcg gcattcccgc
    11941 cattaccgcc gccaccgtca tcattgccgg tctggtcgga cagattgccg gttacaaaat
    12001 gttgaagaac acggtcgtca tgccctcgtc cgtgggtatg tcgctcggca cggcttcgca
    12061 cgcgatgggc attgccgcct cgctcgaacg cagccgccgc atggcggcat acgcggggct
    12121 ggggctgacg ttcaacggcg tgctgaccgc gctgattgcg ccgctgctta tccccgtttt
    12181 ggggttctga acccgtttca gacggcattt caacccatgc cgtctgaacg ccgacacact
    12241 cgcaaggaga accgttatgg ctgtcaacct gaccgaaaaa accgccgaac aactgcccga
    12301 catcgacggc attgccctct acaccgccca agcaggcgtg aagaagcccg ggcataccga
    12361 cctgacactg attgccgtag ccgccggcag caccgtcggt gcagtcttca cgaccaaccg
    12421 tttctgtgcc gcgcccgtcc acatcgccaa atcgcacctt ttcgacgaag acggcgtgcg
    12481 cgccctcgtc atcaacacgg gcaacgccaa cgcgggtacg ggcgcacagg gcagaatcga
    12541 tgctttggca gtgtgtgccg ccgccgcccg gcaaatcggc tgcaaaccga accaggtgct
    12601 gcccttctcc accggcgtga ttctcgaacc gctgcccgca gacaaaatca tcgccgccct
    12661 gcccaaaatg cagcctgcct tctggaacga agcggcacgc gccatcatga ccaccgacac
    12721 cgttcccaaa gccgcctcgc gcgaaggcaa ggtcggcgac caacacaccg tccgcgccac
    12781 aggcattgcc aaaggctcgg gcatgattca tcccaatatg gcgaccatgc tcggtttcat
    12841 cgccaccgat gccaaagttt cccaacccgt cctccaactg atgacgcagg aaatcgccga
    12901 cgaaaccttc aacaccatca ccgttgacgg cgacaccagc accaacgaca gcttcgtcat
    12961 catcgccacc ggcaaaaaca gccaaagcga aatcgacaac atcgccgacc cgcgttacgc
    13021 ccaactcaaa gaattgttgt gcagccttgc gctcgaactc gcccaagcca tcgtccgcga
    13081 cggcgagggc gcgaccaagt tcatcaccgt ccgcgtcgaa aacgccaaaa cctgcgacga
    13141 agcccgccaa gccgcctacg ccgcggcacg ttcgccgctg gtcaaaaccg cctttttcgc
    13201 ctccgacccc aacctcggca ggctgctcgc cgccatcggt tatgccgacg ttgccgacct
    13261 cgataccgac ctcgtggaaa tgtatctcga cgatattttg gttgccgaac acggcggacg
    13321 cgccgcaagc tacaccgaag cacaagggca ggcggtgatg tcgaaggacg aaatcaccgt
    13381 ccgcatcaag ctgcatcgcg gacaagccgc cgccaccgtc tatacctgcg acctgtcgca
    13441 cggatacgtt tccatcaacg ccgactaccg ttcctgaccc gacacggctt cagacggcat
    13501 acataaaatg ccgtctgaac cgtcggacaa cataccatga cctccacatt cccccgccgc
    13561 ctcgcccgca aaatccgcca aacccgccgc ctgtcgcgca aaagcatcgc ctttctgttc
    13621 cttctggcag gttcggcact cgtcgccctg accgcactgt tttttgccca tcttgccgat
    13681 tttgcgctgg aactgaacgc caaactggtt caacaatacc cgtggttcgc atggattgcc
    13741 ctgccgccgg gactgcccgt catcgtctgg ctcacacgca aattcgcccc cttcaccgcc
    13801 ggcagcggca tccctcaagt catcgcctca ctgtcgctgc cctacggcgc acaaaaaacg
    13861 cggctgatac gcctcgggca aacgctgctg aagattccgc tgaccttttt gggtatgctg
    13921 ttcggcgcgt ccatcggacg cgaaggcccg tccgtgcagg tcggcgcggc agtaatgggc
    13981 gcgtggggcg cgtggtgcaa aaaacacggt ttggcgttta agggtatgca ggaaaacgat
    14041 ttgatggcgg cgggcgcggc gggcggtttg gcagcagcgt tcaacgcgcc gctggcgggc
    14101 gtgattttcg ccattgagga actggggcgc ggcatcatgc tgcgctggga gcggcagatt
    14161 ctgctcggcg tactcgccgc cggtttcata caggtcgcca ttcagggcaa caacccgtat
    14221 ttttcgggtt tcaacggcgg cgttttggaa aatattttga tgtggatcgt ggcggcgggg
    14281 ctggtttgcg gcgcggctgg cggcatattt gcccgtatgc tctatcgcgg tgcggcggcg
    14341 tttgcaccgc gcaagatacg cggcttcatc cgcaaccgtc cgctgctgct ggcggcactg
    14401 atggggctgc tgctcgccct gctcggcacg ttctaccaag gcaaaaccta cggcaccggc
    14461 taccacgaag ccgcccaagc cctgcacggc atctacgaag cccccgtcgg actcgccgcc
    14521 gccaaatggc tcgccaccgt attcagctat tgggcaggca ttccgggcgg cattttcact
    14581 ccctcgctga ccataggtgc ggttttgggc gagcatatcg ccgccatcgc cgacatatcg
    14641 cagggtgcaa acatcatcgt cctcatctgc atggcggcat ttttagcggg cgcgacacaa
    14701 tcgccgatta cttccgccgt cgtcgtcatg gaaatgaccg gcgggcaaag cctgctgttt
    14761 tggatgctga ttgcctgcat tttcgcctcg caggtttcgc gccagttttc gccgcgtccg
    14821 ttctaccacg catcgggaat gcgcttccgc cggcgcgtgc ttcaggaaac cgccgcccaa
    14881 accggcaatg cgcccgcaag accgcaagcc gcaaacagca aaacgggaat gccgtctgaa
    14941 aattaaaacg cccccgatca aacgccggca gccgccttga ttcaaatacc gttccgctgc
    15001 gcttgaaatt tcagcaacaa tgccgtctga acgacagaat gcggttttca gacggcattt
    15061 ccccatcccg atattgctca aacaaaaccg aagcgtttgc tataattgca ctttttaccg
    15121 cacacgcacg cccatgtttc ccgatttctc ccaaaccctc tccaaagacc gccacttcct
    15181 gcaatccgcc ttcaaaaatc ccaacaaata cggcggcttg tccaaaatcg aagaaaaata
    15241 ccgaaaatcg cacgaaatct tcttgaaacg tttggccgca ttgccaaaac ccgaattcga
    15301 cgacaccctg cccgttcacg aaaaactcga agaaatcaaa aaaaccgttg ccgaaaatca
    15361 ggtaacgatt atctgcggcg aaaccggttc gggcaaaacc acgcagttgc ccaagatttg
    15421 tttggaactc gggcgtgggg cggcaggatt gatcgggcat acccagccgc gccgtttggc
    15481 cgcgcgttcg gtggcggagc ggattgccga agagctgaaa tccgaaatcg gcagcgcggt
    15541 cggctataaa gtgcgcttta ccgaccacac ctcgcgcgat gcctgcgtca agctgatgac
    15601 cgacggcatc ctgctggcgg aaacccagac cgaccgttat ctcgccgcct acgacacgat
    15661 tatcattgac gaagcgcatg aacgcagcct gaacatcgac ttcctcttgg gctacctaaa
    15721 acaactcctg ccgcgccgcc ccgatttgaa agtcatcatc acatcagcca ccatagacgc
    15781 agaacgcttc tcccgacact tcaacggcgc gcccgtactg gaagtgagcg ggcgcaccta
    15841 tcccgtcgaa atcctctacc gaccgctgac cggcaaagac gaagacgacg cagaagtcga
    15901 gctgaccgac gcgattgtcg atgccgccga cgaattggca cgctacggcg aaggcgatat
    15961 tttagtattc ctgccgggcg aacgcgaaat ccgcgaagcg gcagaagccc tgcgcaaatc
    16021 cacactgcgc cgcaatgacg aaatcctgcc cctgtttgca cgcctgtcgc acgccgaaca
    16081 gcacaaaatc ttccaccctt caggcgcaaa atgccgcatc gtgctggcga ccaacgtcgc
    16141 cgaaacctcg ctcaccgtgc cgggcatcaa atacgtcatc gacaccggcc tcgcgcgcgt
    16201 caaacgctat tccgcgcggg caaaagtgga acaacttcac gtagaaaaaa tctcccaagc
    16261 cgccgcccgc caacgctccg gccgctgcgg acgcgtctca gcaggcgtgt gtatccgact
    16321 gttttcagaa gaagatttta acagccgtcc cgaatttacc gatcccgaaa tcgtccgcag
    16381 caacctcgcc gccgtcatcc tgcgcatggc atcgctgaac ttgggcgacg tagcagcatt
    16441 cccgttttta gaaatgcccg actcgcggta tatcaatgac gggtttcagg tgttgttgga
    16501 attgggggca gtggaggaga attagattga aagcaggcta cctgaaaatg tgaagcagcc
    16561 tgcactttta aggttaaatt gaataaagtt cagatgactt aaacgtagaa aaccaagcgg
    16621 tcagctaact gacggcttgg tttttgaccc acccctgacc ctaattctta caacggtgtt
    16681 tcaggtattc agtttccctt tctcctagct gtattcacag caacagttgt aagccgagat
    16741 tttgatttac ctttgcgcta tgaatgcaaa caggcatttc atagggctta atcaataatc
    16801 atgatgataa atcgtcaggc ttaatattgc ccaatgaata gcattttatt taaggctgct
    16861 ttcctgcctt gggggtttaa ggtattttac catgaagcct gatattcaat aatatcgttt
    16921 acagagaatc catgatttaa caaggcttgc cgaatgagaa aaggtaaaga ttgagtatat
    16981 ttagggaagt tacaacaaaa ttccaaccaa aacctgccca tattttcaat atctttacta
    17041 cttaatttat caaaaaatcg aagta
//
LOCUS       DAXFZR010000050        16764 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000050 DAXFZR010000000
VERSION     DAXFZR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16764
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.050"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            159..1223
                     /locus_tag="ACFJAS_001392"
     CDS             159..1223
                     /locus_tag="ACFJAS_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC3982624.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1364..1687
                     /locus_tag="ACFJAS_001393"
     CDS             1364..1687
                     /locus_tag="ACFJAS_001393"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC3982625.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1761..2084
                     /locus_tag="ACFJAS_001394"
     CDS             1761..2084
                     /locus_tag="ACFJAS_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC3982626.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2131..2334
                     /gene="tatA"
                     /locus_tag="ACFJAS_001395"
     CDS             2131..2334
                     /gene="tatA"
                     /locus_tag="ACFJAS_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC3982627.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2338..3024
                     /gene="tatB"
                     /locus_tag="ACFJAS_001396"
     CDS             2338..3024
                     /gene="tatB"
                     /locus_tag="ACFJAS_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC3982628.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDIMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3037..3807
                     /gene="tatC"
                     /locus_tag="ACFJAS_001397"
     CDS             3037..3807
                     /gene="tatC"
                     /locus_tag="ACFJAS_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC3982629.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARSYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3851..4459
                     /locus_tag="ACFJAS_001398"
     CDS             3851..4459
                     /locus_tag="ACFJAS_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC3982630.1"
                     /translation="MNTLYLGSGSPRRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4468..4821
                     /locus_tag="ACFJAS_001399"
     CDS             4468..4821
                     /locus_tag="ACFJAS_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC3982631.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4811..6739
                     /locus_tag="ACFJAS_001400"
     CDS             4811..6739
                     /locus_tag="ACFJAS_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC3982632.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANIPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCKRPASADADTARNVKLHARHC
                     KKQQTGREIRYQKTLQPC"
     gene            6815..7492
                     /gene="misR"
                     /locus_tag="ACFJAS_001401"
     CDS             6815..7492
                     /gene="misR"
                     /locus_tag="ACFJAS_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC3982633.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7505..8911
                     /gene="misS"
                     /locus_tag="ACFJAS_001402"
     CDS             7505..8911
                     /gene="misS"
                     /locus_tag="ACFJAS_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC3982634.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9029..11451
                     /locus_tag="ACFJAS_001403"
                     /pseudo
     CDS             9029..11451
                     /locus_tag="ACFJAS_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11496..11741
                     /gene="rpsP"
                     /locus_tag="ACFJAS_001404"
     CDS             11496..11741
                     /gene="rpsP"
                     /locus_tag="ACFJAS_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC3982635.1"
                     /translation="MVVIRLARGGSKHRPFYDVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11757..12266
                     /gene="rimM"
                     /locus_tag="ACFJAS_001405"
     CDS             11757..12266
                     /gene="rimM"
                     /locus_tag="ACFJAS_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC3982636.1"
                     /translation="MTDAQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12266..13015
                     /gene="trmD"
                     /locus_tag="ACFJAS_001406"
     CDS             12266..13015
                     /gene="trmD"
                     /locus_tag="ACFJAS_001406"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC3982637.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13030..13395
                     /gene="rplS"
                     /locus_tag="ACFJAS_001407"
     CDS             13030..13395
                     /gene="rplS"
                     /locus_tag="ACFJAS_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC3982638.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13716..14441
                     /locus_tag="ACFJAS_001408"
     CDS             13716..14441
                     /locus_tag="ACFJAS_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC3982639.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTSQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14476..15351
                     /locus_tag="ACFJAS_001409"
     CDS             14476..15351
                     /locus_tag="ACFJAS_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC3982640.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15428..16342
                     /locus_tag="ACFJAS_001410"
     CDS             15428..16342
                     /locus_tag="ACFJAS_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFC3982641.1"
                     /translation="MKHLKLTLITALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            16562..>16764
                     /locus_tag="ACFJAS_001411"
     CDS             16562..>16764
                     /locus_tag="ACFJAS_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC3982642.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDIMTDKGKWKLETSLTYLN
                     SENSRSELAAPVYIQTGSASFIPV"
BASE COUNT         4366 a         5336 c         3685 g         3377 t
ORIGIN      
        1 tgtcgggagg gaagggaatt ttgcaaaggt ctcagtttgt agtaaaatat ctaaacagat
       61 tcaatcggaa acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgctagat
      121 tcagacggca ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttaca
      181 acaaaggcga catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcaccgtcg
      241 gcatcaatgt cgcctggtgc ggcatctgcg gtaccgacct gcacgaattt atggaaggcc
      301 cgattttcat cccgccctgc ggtcatccgc atccgatttc cggcgagtcc gcgcccgtaa
      361 cgatgggaca cgagttctcc ggcgtggttt atgccgtcgg cgaaggcgtg gacgacatta
      421 aagtcggcca acacgtcgtg gtcgaaccct acatcatccg cgacgacgta ccgaccggcg
      481 aaggcagcaa ctaccacctc tccaaagaca tgaactttat cggcttgggc ggctgcggcg
      541 gcggcctgtc cgaaaaaatc gccgtcaaac gccgttgggt gcatccgatt tccgacaaaa
      601 tcccgttgga tcaagctgct ttgattgaac ctctgtctgt cggacaccac gcctatgtac
      661 gcagcggcgc gaaagcaggc gatgtcgcat tggtcggcgg cgcaggcccg atcggtttgc
      721 tgttggccgc cgtgttgaaa gccaaaggca tcaaagtcat cattaccgag ttgagtaaag
      781 cacgcaaaga caaagcccgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag
      841 tcgatgttgt tgaagaagtg aaaaaactga ccaacggcga aggcgtggac gtggcgttcg
      901 aatgtaccag cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cccgccgcca
      961 acttggttat cgtatccatc tggagccacc ccgccaccgt caacgtccac agcgtcgtga
     1021 tgaaagaact ggatgtgcgc ggcacgattg cctactgcaa cgaccacgcg gaaaccatca
     1081 aactggtcga agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg
     1141 acaaactggt ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa
     1201 ttattgtgaa tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa
     1261 acgctcagac ggcattttcc aacgaaacgc aacaggcgta ttgcccgaga gcagccgttt
     1321 agcctatcat tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact
     1381 atccgccatc caacaaacca ttatacagcg caaatctgcc gacccgtccg aatcttacgt
     1441 cgcacagctc ctgcacaagg gcgaggacaa aatcctaaaa aaagtgattg aagaagcggg
     1501 cgaagtgttg atggcatcca aagacaaaga cccgtcccac ctggtttacg aagttgccga
     1561 cttatggttc cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt
     1621 ggacgaactt tcccgccgtc aggggctgtc ggggctggcc gaaaaagccg cccgtacaga
     1681 atcctgaatt tatattacaa tctcatccct tccacgttta atccgtctgc gtgctttttt
     1741 cagacggcaa cggagccgtt atggacaact gtattttctg caaaatcgcc gccaaagaga
     1801 ttccggcgca aaccgtctat gaagacggcg aaatggtttg tttcaaagac atcaaccccg
     1861 ccgctccgct tcatctgctg ctgattccca aagtccattt tgattcgttg gcacacgccg
     1921 cgcccgaaca tcagcccctt ttgggaaaaa tgatgctgaa agttcccgaa atcgccaaag
     1981 cttcgggact gaccgacggt ttcaaaaccc ttatcaatac cggcaaaggc ggcggacaag
     2041 aggtcttcca cctgcatata cacatcatgg gcacacccgt ataaaccgtt atttcacaat
     2101 caacccctaa tacttactta aggatacatc atgggcagtt tttctctgac gcactggatt
     2161 atcgtactga ttatcgtcgt tttgatattc ggcaccaaaa aactgcgcaa cgtcggcaaa
     2221 gacctcggcg gtgcggttca tgacttcaaa caggggctga acgaaggcac agacggcaaa
     2281 gaagcccaaa aagacgatgt aatcgaacac aaaaaagacg aagacaaagc ataacttatg
     2341 tttgatttcg gtttgggcga gctgattttt gtcggcatta tcgccctgat tgtccttggt
     2401 ccagaacgcc tgcccgaagc cgcccgcacc gccggacggc tcatcggcag gctgcaacgc
     2461 tttgtaggaa gcgtcaaaca agaatttgac actcaaatcg aactggaaga gctgaggaag
     2521 gtcaagcagg cattcgaagc tgccgccgct caggttcgag acagcctcaa agaaaccgat
     2581 acggatatgc agaacagtct gcacgacatt tccgacggtc tgaagccttg ggaaaaactg
     2641 cccgaacagc gcacgcctgc cgatttcggt gtcgatgaaa acggcaatcc ccttcccgat
     2701 acggcaaaca ccgtatcaga cggcatttcc gacattatgc cgtctgaacg ttccgatact
     2761 tccgccgaaa cccttgggga cgacaggcaa accggcagta cagccgaacc tgcggaaacc
     2821 gacaaagacc gcgcatggcg ggaatacctg actgcttctg ccgccgcacc tgtcgtacag
     2881 gccgtcgaag tcagctatat cgatactgct gttgaaacgc ctgttccgca caccacttcc
     2941 ctgcgcaaac aggcaataaa ccgcaaacgc gattttcgtc cgaaacaccg cgccaaaccg
     3001 aaattgcgcg tccgtaaatc ataaaggggg catccggtgt ccgaaacaca aaacgaacaa
     3061 accgtccaac cgcttgtcga acacctcatc gagctgcgcc gccgcctgat gtggatggtt
     3121 gtcggcattc tcgtctgctt tttcggcatg atgccgtttg cccagcagct ctatactttt
     3181 atcgccgacc cgctgatggc aaacctgccc aaagacacca gtatgattgc gaccgatgtc
     3241 atcgcaccgt ttttcgttcc ggtcaaagtt accctgatgg cggcgttttt ggtttcactg
     3301 ccgcatacgc tctaccaaat ctgggcattc gtcgcccccg cactctacca aaacgaaaaa
     3361 cgcctgatta cgccgctcgt cctctccagc gtcagcctgt ttttcatcgg catggcattt
     3421 gcctatttcc tggttttccc cgtcattttc aaattccttg ccggcgtaac ccccgtcggg
     3481 gtcaatatgg cgaccgacat agacaaatac ctgtccttta tcttaggtat gttcgttgcg
     3541 ttcggcacgg catttgaagt gcccatcgtc gtcatcctgt taaccaaaat cggcgcagtc
     3601 acgaccgagc agctcaaaca cgcccggtct tatgtcattg tcggcgcgtt tgtcgttgcc
     3661 gcagtcatca cgccgcccga cattatttct cagaccctgc ttgccattcc gctgatcctc
     3721 ttatacgagg cgggcatttg gttcggacgc ttcttcactc cacgttcaga acaggatggc
     3781 gacatacagc cgcctgcaaa aacctgacac tatgccgtct gaagccccgc cgcacattaa
     3841 ggagcacacc gtgaacaccc tttacttagg ttcgggcagc ccgcgccgga tggaaatcct
     3901 gacacagttg ggctatcggg tcgtcaagct gcccgccggc atcgacgaaa ccgttaaagc
     3961 cggcgaaacg cccgcccctt acgtccaaag gatggcggaa gaaaaaaatc aggcggcttt
     4021 gaccctcttt tgcgaaacca acggcgcaat gcccgatttc cccctgatta ccgccgacac
     4081 ctgcgtcttt tcagacggca tcatattggg caaacctcgc tcccaagccg aagcaatcga
     4141 atttttaaac cgattgtccg gcaaacaaca taccgtcctg actgctgtct gcattcatta
     4201 tcgcggcaaa acgtcaagcc gcgtccaaac caaccgcgtc gttttcaagc ccctgagttc
     4261 ggaagaaatt tccgcctatg tgcaaagcgg cgaaccgatg gagaaagccg gtgcctacgc
     4321 cgtgcaaggc ataggcggta tctttatcca atctatcgaa ggcagcttca gcggcattat
     4381 ggggctgccc gtttatgaaa ccgtttccat gttgcaggat ttgggatacc gccccccctt
     4441 atccgccctt aaaccgtaaa gtccgccgtg aacagacaaa ccgcttactt ccttgcctcc
     4501 ttcagcctga ttgcactgat tgccctatcc ctttcttggg aattatggat tgcaccgtta
     4561 cgcccgggcg gttcgtggct cgcgctcaaa gccctgcccc tctgcctgcc gctttcaggt
     4621 attctaaaga aaaaaatcta tacctaccaa tacagctcca tgctggttct gatttacttt
     4681 gccgaagccg tcatgcgcct gttcgacgcc tatcccgcag aaaaaatttg cgccgcgctt
     4741 tccgcagtat tcagcatcat cttcttcata tcctgcctgt cgttcgtcaa acaatacaag
     4801 gaaacaaaca atgtccgctg aaacgaccgt atccggcgcg cgccccgccg ccaaactgcc
     4861 gatttacatc ctgccctgct tcctttggat aggcatcatc ccctttacct tcgcactcag
     4921 gctgaaaccg tcgcccgact tttaccacga tgccgccgcc gcggccggcc tgattgtcct
     4981 gttgttcctc acggcaggaa aaaagctgtt tgatgtcaaa atccccgcca tcagcttcct
     5041 cctgtttgca atggcggcat tttggtggct tcaggcacgc ctgatgaacc tgatttatcc
     5101 cggaatgaac gacatcgcct cttgggtttt catcttgctc gccgtcagcg cgtgggcctg
     5161 caagagtttg gtcgcacact acggacaaga acgcatcgtt accctgtttg cctggtcgct
     5221 gcttatcggc tccctgcttc aatcctgcat cgtcgtcatc cagtttgccg gctgggaaaa
     5281 cacccccctg cttcaaaaca tcatcgttca cagagggcaa ggcgtaatcg gacacatcgg
     5341 gcagcgcaac aacctcggac actacctcat gtggggcata ctcgcctccg cctacctcaa
     5401 cggacaacga aaaatccccg cagccctcgg cgcaatctgc ctgattatgc agaccgccgt
     5461 tttaggtttg gtcaattcgc gcaccatctt gacctacata gccgccatcg ccctcatcct
     5521 tcccttctgg tatttccgtt cggacaaatc caacagacgg acgatgctcg gcatagccgc
     5581 agccgtattc cttaccgcgc tgttccaatt ttccatgaac gccattctgg aaacctttac
     5641 aggcatccgc tacgaaactg ccgtcgaacg cgtcgccaac ggcggtttca cagacttgcc
     5701 gcgccaaagc gaatggaata aagcccttgc cgccttccag tccgccccga tattcgggca
     5761 cggctggaac agttttgccc aacaaacctt cctgatcaat gccgaacagc acaccataca
     5821 cgacaacttc ctcagcacct tgttcaccca ttcccacaac atcatcctcc aactccttgc
     5881 agaaatgggg atcagcggca cgcttctggt tgccgcaacc ctgctgacgg gcattgccgg
     5941 gctgctgaaa cgctccctga cccccgcatc acttttcctg ctgtgcgcgc ttgccgtcag
     6001 tatgtgccac agtatgctcg aatatccttt gtggtatgtc tatttcctca tccccttcgg
     6061 actgatgctc tttctgtccc ccgcagaggc ttcagacggc atcgccttca aaaaagccgc
     6121 caatctcggc atactgaccg cctccgccgc catattcgca ggattgctgc acttggactg
     6181 gacatacacc cggctggtta actccttttc ccccgccgct gacgacagtg ccaaaaccct
     6241 caaccggaaa atcaacgaac tgcgctatat ttccgcaaac atcccgatgc tgtcctttta
     6301 tgccgacttc tccctcgtaa acttcgccct gccggaatac cccgaaaccc agacttgggc
     6361 ggaagaagca accctcaaag cactaaaata ccgcccctac tccgccacct accgcatcgc
     6421 cctctacttg atgcggcaag gcaaagttgc agaagcaaaa caatggatgc gggcaacaca
     6481 gtcctattac ccctacctga tgccccgata cgccgacgaa atccgcaaac tgcccgtatg
     6541 ggcaccgctg ctgcccgaac tgctcaaaga ctgcaaagcc ttcgccgccg cgcccggcca
     6601 tccggaaaca aaaccctgca aacgacccgc gtcggcggat gcggataccg cccgaaatgt
     6661 aaagctccat gcaagacatt gcaaaaaaca gcaaaccggt agggaaatac gctatcaaaa
     6721 aacattgcag ccgtgttaag ataaactgtc aaacaatctt ttcacgccac gcccgaaaca
     6781 gggtcggggc atacccttac gaaaaggaaa caccatgagc cgcgtattac tcgtagatga
     6841 cgatgccctg ctgaccgaac tgctgaccga atacctgagc gccgaaggtc tgaacgtccg
     6901 cagcgttccc gacggggaag caggcgtaca ggaaatcctg agcgggcaat acgatgtagt
     6961 cgtattggat tccatgatgc ccaaaatgaa cggcttggat gtcttgaaaa acgtacgcgc
     7021 ccgaagcacc gtccccatca tcatgctgac cgccaaaggc gacgacatcg accgaatcat
     7081 cggcttggaa atgggtgcgg acgactatgt ccccaaaccc tgcacaccac gcgaactctt
     7141 ggcacgcatc aatgccatcc tccgccgagc acaacacagc ggcgaacaga acaacgcacc
     7201 caacagcatc tccgtcagcg atgtcgtcct ataccccgcc aaacgccagg catccgtcaa
     7261 agacatgccg ctcgaactga ccagcaccga attcaacctg ctcgaagtcc tgatgcgcca
     7321 tgccggacag gtagtcagca aagaaaccct gtccgtcgaa gcactcgacc gcaaactggc
     7381 aaaattcgac cgcagtatcg acgtacacat ctccagcatc cgccacaagc tgggcgatgc
     7441 ctccctgatt caaaccgtac gcggcttggg ctacctgttt gtcaaaaact gaagcagcgg
     7501 gcaaatgaaa ctgttccaac gcattttcgc cacattttgc gcggttatcg tctgcgcaat
     7561 ctttgtggcg agtttttctt tttggctggt gcagaacacc cttgccgaaa accaattcaa
     7621 ccaacgccgc accatcgaaa ccacattgat gggcagcatt atttccgcat tcaagacacg
     7681 gggcgacaac ggcgcgcgcg aaatcctgac cgaatggaaa aacagccccg tctcatccgc
     7741 cgtttacgtc atacagggcg acgagaaaaa agacatctta aaccgctata tcgacaatta
     7801 caccatagaa cgcgcccggc tgtttgccgc caacaacccc cattccaacc ttgtccgcat
     7861 cgaatacgac cgtttcggcg aagaatacct gttcttcatt aaaggctggg acaaccacca
     7921 ggcacaacgc ctgcccagcc cgctgtttat cccgggcctg ccgcttgccc cgatttggca
     7981 cgaattcatc atcctctcct tcatcatcat tgtcggactg ctgatggcat atatccttgc
     8041 cggcaacatt gccaaaccca tcagaatctt aggcaacggc atggacaggg tggcaaacgg
     8101 agaacttgaa acccgcatct cccaacaggt cgacgaccgc gacgacgaat tgtcccatct
     8161 tgccatccaa ttcgacaaaa tggcagaaaa actcgaaaaa cttgtcgcca aagaacgtta
     8221 cctactccac cacgtttccc acgaaatgcg ctccccgctt gcccgtatgc aggcaattgt
     8281 cggactgatt caggcacagc cccaaaaacg ggagcaatat ctcaaacggc tggaaggcga
     8341 actgacccgc atggatacgc tggccgggga actgttaacc ctgtcccgcc tcgaaacctc
     8401 caatatggct ttggaaaaag aaagcctgaa actcctgccc ttcctgggca acctggtaga
     8461 agacaaccaa agtatcgccc aaaagaacgg acagacggtc gccctgtctg ccgacggaaa
     8521 aatccccgaa aacacaacca tccttgccaa cgaaagctac ctgtaccgcg ccttcgacaa
     8581 cgtcatccgc aacgccgtca actacagccc cgaaggcagc accatcctga tcaacatcgg
     8641 acaagaccac aaacactgga taatcgacgt taccgacaac ggccccggcg tggacgaaat
     8701 gcagctcccg cacatcttca ccgctttcta ccgtgcagac tccagtgcca acaaacccgg
     8761 aacaggactg gggcttgcat tgacccaaca tattattgaa cagcactgcg gcaaaatcat
     8821 cgccgaaaac atcaaaccga acggtctgcg gatgcgcttt atcctgccca agaaaaaaac
     8881 cggtttcaaa acagaaaaaa gtgcgaactg accataatgc cgtctgaacc cgcttcagac
     8941 ggcattgcac aaacagttac cccaaaacaa acacacagcg ggtgcatctc caaaaatcac
     9001 gcacataccg tccgcaaagg aatatatcat gccggcacaa accgatccgg gctacttctt
     9061 catgcccaac cacattatcc tgataggcgc gagcgaacaa ccgtacagcc tgggtgaacg
     9121 cgtactcagc aacctgctga gtacgccctt tcaaggaaaa atcacccccg taaacccgcg
     9181 ccaccacacc atagccgggc ttcccgccta caccagcctc aacaaaatcc ccggcagtgc
     9241 agacctgatt attaccgtta ccccgcccga cagttacgac accctcctca aaacctgccg
     9301 taaaaagcag ctccgacaca tcatcctcat acaggactgg gacagcctgt ctgccgcaga
     9361 actgcacacc gccgaaactg ccatccgcaa acaccacggc aacggactca acatcaccgc
     9421 ctgcaccacc gcaggcatcc aactgccctc actcggactc aacatcagta cccaagacgg
     9481 atatgccgca ggccataccg ccatactgac cggcaatgcc gccgtcagcc gccaaatcga
     9541 caacatcctg aacaaactcc gtcaaggcac atcccgccac atcagcctgc atcccggcat
     9601 cagccccatc acatccgccg attggctcaa ccgcttcgga cacagcctgc acaccaaaac
     9661 cgccgtccta caccacaacc ctgaagagga tcagcgcagg ctgttcagcg caatccgcca
     9721 atttacccgc catacgccgc tgattctcca catcacctgc ctcacgacag aaaccgaccg
     9781 tgccgtactg cactgcctcg cccgacactg caacttcctc atcagtttca acgccgacga
     9841 cctcgaagcc gcactgagcg cccaactgtc cgaccttcca cccctgtccc gactcgacat
     9901 cctgtccgac acgcctgccg aatggctgca cgcgcacgca ccaaaaaacc ccaccctcca
     9961 ctttcccaac cttccccacc acatccgcaa cggacacctg accggcacac ccacaccctc
    10021 aatctgccac gacatcgcct cacgtcagct tgcccgcccg acacccaagc cgtcctgacc
    10081 atcctcggtc cctccggaca cgaggattac aaaaaaacag cacgcgccct tatccgcctg
    10141 tccgaacaga ccgccaaacc cctgctcgtc aacagcccct tttcagacgg cataacacat
    10201 ttcgacaccc ccgcacaggc aatccgcacc ctttcctacc gtaacaccgc cgccgccctg
    10261 aaacaggcac agctcgacat tgcaccgccg cagccatgcc gtctgaaaac accgcaaccc
    10321 caaaacatca aaaaagccct tgcagcggca aatcccgccc tgctcgccga agccctgcac
    10381 ctccccctac cggcacacca cccataacgc cgtccaattc caattcggca gccaccccct
    10441 ctatggcgac atcctgaccg cacgctgcaa cggacaaacc actgccgtac tcccgccgtt
    10501 taccacgctc gacagccgcc accttgcccg ctttgccgaa ctcgacggca cacaaaccct
    10561 cgaccagttc ctgcacacac tgaccgtcat ccccgaatac cgccaacaca ttttcggcat
    10621 caccctcaac ctcaacggcg gacaatacag cagcgatttc atcttaaaga cacccggaac
    10681 acacgacacg cccaaacgca agaacacagg caaagccgcc caaaccctcg aacatgccgc
    10741 cgcaaaaatg cagagtgccg ccgcatacct ggaacacaaa aacccgacag ccgccgaatt
    10801 tctccgccac acaagcgaag ccgccgcaga actgctcggc agcaaaaccg aaaccggagc
    10861 agccgtaccc aacgtacttg ccccctatcc cgcagcacac cccaaaacac tgtccctaaa
    10921 aaacaacacg accgttacca ttatccccct tttacccgaa gacgcagaag ccaaacagca
    10981 gttcgtccgc agcctcggcc ccgaagcacg gtacacacgc ttcatgaccc acaccaacga
    11041 actgcccgca gccacgttgg cacgcctgtg caaccccgat taccactgtg aaccgcatgg
    11101 acggcaaggg atgccgacag caacatcgtc gccgtcgtcc gccacagccg cctgaaccgc
    11161 aacgaatgcg aatttggcat cacactggcg gaacatatgc gcggcagcgg gctggcacag
    11221 aaaatgatgg aactcatcat ccaaaccgcc gcacagcaag gctacctgag catgagtgcc
    11281 gacattctca aaaccaatac ccccatgatc aaacttgctg aaaaatcagg atttaccctc
    11341 aaggaatcgg acaccgaaaa aaacctgtac cgcgcatatc tgaaccttgc ggcagacaaa
    11401 acaacaggaa aaacaaataa aaacttgcgc accggccaca aaataaccta aaatcggcag
    11461 tttccatata tccgattttc tcaaaaggac tcaaaatggt agttatccgt ttggcacgcg
    11521 gcggctcgaa acaccgcccc ttctacgacg tcatcgttac tgactcacgc agccgccgcg
    11581 acggccgctt catcgaacgc gtaggcttct acaaccccgt agccaacgaa aaacaagagc
    11641 gcgtccgcct caatgcagac cgcctgaacc actggattgc acaaggcgcg caagtcagcg
    11701 actccgttgc aaaactgatt aaagaacaaa aagccgtcta atccgcgttt gccgccatga
    11761 cagacgctca aaaccgggta gccatgggct acatcaaagg cgtattcggc ataaaaggct
    11821 ggctgaaaat tgccgccaac accgaatatt ccgacagcct tttggactac cccgagtggc
    11881 atttggccaa ggacggcaaa accgtcagcg ttacccttga agccggaaaa gtcgtcaacg
    11941 gcgaactcca agtcaaattc gaaggcatag acgaccgcga ttcagcattc tcattgcgcg
    12001 gttacaccat cgaaataccc cgtgaagcat tcgccccgac agaagaagac gaatactact
    12061 gggcagactt ggtcggcatg accgtcgtca acaaagacga taccgtttta ggcaaggtaa
    12121 gcaacctgat ggaaaccggc gcaaacgacg tattgatgat tgacggagaa cacgggcaga
    12181 ttctgattcc gttcgtttcc caatatatcg aaaccgtcga taccggcagc aagaccatta
    12241 ctgccgactg gggtttggac tactgatgct tatccaggca gttaccattt tccccgaaat
    12301 gttcgacagc attacccgct acggcgtaac gggacgcgcg aacagacagg gaatctggca
    12361 gtttgaagca gtcaatcccc gaaagtttgc cgacaacaga ttgggctata tcgacgaccg
    12421 cccgttcggc ggcggtccgg gaatgattat gatggctccg ccgcttcatg cggcgataga
    12481 acacgccaaa gcacaatctt cccaaaccgc aaaagtcatc tacctcagcc cccaaggaaa
    12541 accgctgaca caccaaaaag cggcagaact ggcagaactt acgcatctga ttctgctgtg
    12601 cggacgctat gagggaatag acgaaagact gctgcaaagc agcgtcgatg aagaaatcag
    12661 catcggagac ttcgtcgttt ccggcggaga gcttcccgcc atgatgctga tggatgcggt
    12721 attgaggctc gtacccggca tattgggcga cattcagtct gccgaacagg attcgttctc
    12781 aagcggtatt ttggactgcc cccactacac caaaccctta gaatttcaag gcatggctgt
    12841 tccggaagta ttgcgctccg gaaatcatgg cttgatagcg gaatggcggt tggaacaatc
    12901 gctgcgccgc accttggagc gcagacccga tcttttggaa aagcgcgttt taatcccaaa
    12961 ggaatcccgc ctcttgaata aaatcctaca agagcaacgg gaaatccaat cataatttag
    13021 gaaaaaacaa tgaacctgat tcaacagctc gagcaagaag aaattgcccg cctgaacaaa
    13081 gaaatccccg aattcgcacc gggcgacacc gtagtcgtat ccgtacgcgt cgtggaaggt
    13141 acccgcagcc gtctgcaagc ctacgaaggc gtggttatcg cccgtcgcaa ccgtggtttg
    13201 aacagcaact tcatcgtccg caaaatctcc agcggcgaag gtgttgaacg tactttccaa
    13261 ctatattccc ctactgttga gaaaatcgaa gtcaaacgcc gtggcgacgt acgccgtgcc
    13321 aaactgtact acctgcgcgg tctgaccggc aaagctgcac gcatcaaaga aaaactgcct
    13381 gcacgcaaag gttgattcaa accgttttcc cccaatgccg tctgaacgtt cagacggcat
    13441 tttttattgc cgcgatgccg gcaaggcaac gatatgcctg accctcatcc accttaatta
    13501 acaatcactc ccgcctattc aagcagaatt tacagactaa acgttataca gtatcatatc
    13561 cgccatataa cgataaccgc tttgccaact tccctttcaa aactgcaaat cattatgaca
    13621 ccttccctct tactgtcagg attgaccttc cgactcttcc tcgccctgat tgccgtattc
    13681 cttttatggg gtgtttacct ttgggcggta tcggtatgag catcatcgtt gaaaacctga
    13741 cggtcagcta ccgccgccgc cccgccgtac accacgtcga cattactttt gaagaacata
    13801 gtatgtgggc ggttttcggt cccaacggcg caggaaaatc tacctttctc aaatccttaa
    13861 tgggattgca gcctatcgat acgggcagca tccggctgga cggattgacc agtcaaaaca
    13921 ttgcctatct cccccaacaa tccgacatcg accgttccca gcctatgacc gttttcgact
    13981 tggcggcaat ggggctgtgg tatgaaatcg gttttttcaa agggataaat accgctcaaa
    14041 aacaacgcgt tcacgaagcc ttggagcgtg tcggtatgcg gcagtttgcc cgccgccaaa
    14101 tcgcccacct ctcaaacgga caattccaac gcgtcctttt cgcccggatg ctggttcaaa
    14161 atgcaaaatt cctgcttttg gacgaacctt tcaatgctgt tgatgcacgg acaacctacg
    14221 agcttctcga cgtgttgcaa aaatgccatt gcggcggaca cgctatcatt gccgttttgc
    14281 acgattacga acaagtccgt gcctactttc ccaataccct gctgctcgcc cgcgaaaaaa
    14341 ttgcggcagg cgcaaccgag accattctga cagaatccta cctcgcccaa gccagcgcca
    14401 aaatgcagca acaggaaagc cccgactggt gcgcctcata aatactgccc gaaaccacgc
    14461 cccacaccag acaccatgaa cctctacgac ctgctccttg ccccctttgc agaattcgac
    14521 ttcatgcact acgccctcgc atccgtcttc tgcctgtccc tcagcgccgc acccgtcggc
    14581 gtattcctcg tcatgcgccg tatgagcctg ataggcgacg cattgagcca cgccgtcctg
    14641 cccggtgccg ccgtcggcta catgtttgcc ggcttgagcc tgcccgctat gggtgtgggc
    14701 gggtttgccg ccggtatgct gatggcgctg cttgccggac tcgtcagccg ctttaccacc
    14761 ctgaaagaag atgccaactt tgccgccttt tacctgagca gcctcgccat cggcgtaatc
    14821 ctcatcagca aaaacggcag cagcgtcgat ttactccacc tccttttcgg atctgtgctt
    14881 gccgtcgata ttcccgcact gcaactcatc gccgccgtct ccggcctcac gctcattacc
    14941 cttgccgtca tctaccgccc cctggtgcta gaaagcatag accccctgtt cctcaagtcc
    15001 gtcaacggca aaggcgggct ttggcacgtc gttttcctca tcctcgtcgt tatgaacctc
    15061 gtatccggct tccaagctct cggcaccctg atgtcggtcg gacttatgat gctgcccgcc
    15121 attaccgccc gcttatgggc aagaaatatg gggacgctca ttctgttgtc cgtcctcatc
    15181 gccctttttt gcggtttgat cgggctgctc atttcctacc acatcgaaat cccttccggc
    15241 cccgccatca tcctctgttg cagcgtcctt tatctctttt ccgtcatact cggcaaagaa
    15301 ggcggcatct tgcccaaatg gttcaaaaac caccgccacc acaccacctg accccgcaag
    15361 ccgcttgaac aaacaggcgg cttgttttac cataatgaat gttatgttat atcacagaag
    15421 aacagttatg aaacacctca aactcaccct tattaccgca ttgctggcca ccgccgcaac
    15481 tgccgcaccc cttccggttg taaccagttt cagcatttta ggcgacgtag ccaaacaaat
    15541 cggcggtgag cgcgtagccg tacaaagcct cgtcggagcc aaccaagata ctcatgccta
    15601 tcacatgacc agtggcgaca ttaaaaaaat ccgcagtgca aaactcgtcc tgctcaacgg
    15661 cttgggactt gaagccgccg acatccaacg cgccgtcaaa cagagcaaag tatcctatgc
    15721 cgaagcgacc aaaggcatcc aacccctcaa agccgaagaa gaaggcggac accatcacga
    15781 ccacgatcat gaccacgaag gacaccacca cgaccacggc gaatatgacc cccacgtctg
    15841 gaacgaccct gttcttatgt ccgactatgc ccaaaacgtc gctgaagccc tgataaaggc
    15901 cgatcccgaa ggcaaagttt attatcaaca acgcttgggc aactaccaaa tgcagcttaa
    15961 aaaactgcac agcgacgcac aagccgcatt taatgccgtc cctgccgcca aacgcaaagt
    16021 cctgaccggg cacgacgcat tttcctacat gggcaaccgc tacaacatca gcttcatcgc
    16081 cccgcaaggc gtgagcagcg aagccgagcc gtccgccaaa caagtcgccg ccatcatccg
    16141 gcaaatcaaa cgcgaaggca tcaaagccgt atttaccgaa aatatcaaag acacccgcat
    16201 ggttgaccgc atcgccaaag aaaccggcgt caacgtcagc ggcaaactgt attccgacgc
    16261 actcggcaac gcgcccgcag acacctacat cggcatgtac cgccacaacg tcaaagcctt
    16321 gaccaacgcg atgaagcaat aaaaccgttt gattggtttg aatttgaaaa ggcaaaaggc
    16381 cgtctgaaaa ttaaatacga ggttttcaga cggccttctc ttgtgcaata aagcagtaaa
    16441 caaatattga taagaaaaaa acaatcagta ttgaaaatat cgttaaaaat attatttaga
    16501 cagattacat ggaatttcat taacatcagt agactttgat ttcaatcaag aaaacaatgt
    16561 catgaaaaat attctcttag tatttgttag ctttgtgcca ttatgtgtcc gcactgatct
    16621 gccgctgaat atcgaagaca taatgaccga caagggaaaa tggaaactgg aaacttccct
    16681 tacctacctg aatagcgaaa acagccgttc cgaacttgcc gcaccggttt acattcagac
    16741 cggctccgct tcctttatcc ccgt
//
LOCUS       DAXFZR010000051        16733 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000051 DAXFZR010000000
VERSION     DAXFZR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16733
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.051"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(43..1605)
                     /gene="aceF"
                     /locus_tag="ACFJAS_001412"
     CDS             complement(43..1605)
                     /gene="aceF"
                     /locus_tag="ACFJAS_001412"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC3982643.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     FLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSASPAAAKIDEAAFAKA
                     HAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKPAAAGASLGG
                     GLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEF
                     RKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADT
                     PNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTG
                     FTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFL
                     ANLLKDFRRITL"
     gene            complement(1755..4418)
                     /gene="aceE"
                     /locus_tag="ACFJAS_001413"
     CDS             complement(1755..4418)
                     /gene="aceE"
                     /locus_tag="ACFJAS_001413"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC3982644.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            5115..6827
                     /locus_tag="ACFJAS_001414"
     CDS             5115..6827
                     /locus_tag="ACFJAS_001414"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC3982645.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            6890..7546
                     /locus_tag="ACFJAS_001415"
     CDS             6890..7546
                     /locus_tag="ACFJAS_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC3982646.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(7556..8011)
                     /gene="ruvX"
                     /locus_tag="ACFJAS_001416"
     CDS             complement(7556..8011)
                     /gene="ruvX"
                     /locus_tag="ACFJAS_001416"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC3982647.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(8004..8552)
                     /locus_tag="ACFJAS_001417"
     CDS             complement(8004..8552)
                     /locus_tag="ACFJAS_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC3982648.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(8554..9114)
                     /locus_tag="ACFJAS_001418"
     CDS             complement(8554..9114)
                     /locus_tag="ACFJAS_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC3982649.1"
                     /translation="MNRLLLLSAAFLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGASFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
     gene            9440..11236
                     /locus_tag="ACFJAS_001419"
     CDS             9440..11236
                     /locus_tag="ACFJAS_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC3982650.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKHTEEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKA
                     QISRFVSGNYKNSRPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKISKDARKLLEQKG
                     NEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMS
                     NLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYS
                     TAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAGSK
                     IGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            11363..12838
                     /locus_tag="ACFJAS_001420"
     CDS             11363..12838
                     /locus_tag="ACFJAS_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC3982651.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
     gene            13168..15195
                     /gene="uvrB"
                     /locus_tag="ACFJAS_001421"
     CDS             13168..15195
                     /gene="uvrB"
                     /locus_tag="ACFJAS_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC3982652.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLIEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(15373..16131)
                     /gene="cah"
                     /locus_tag="ACFJAS_001422"
     CDS             complement(15373..16131)
                     /gene="cah"
                     /locus_tag="ACFJAS_001422"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFC3982653.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
     gene            complement(16540..>16733)
                     /locus_tag="ACFJAS_001423"
     CDS             complement(16540..>16733)
                     /locus_tag="ACFJAS_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982654.1"
                     /translation="ISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSS
                     KILFSSHNKIKEFNSNKYD"
BASE COUNT         4115 a         4169 c         4590 g         3859 t
ORIGIN      
        1 caatccaaac attatcagac tgcttgagag ggatgtttta ttttataagg taatgcggcg
       61 gaagtctttc aacaggttcg ccaggaatac ggtgaagcgc ataccggcgg caccgtcgat
      121 gacgcggtgg tcgaaggaca ggctcaacgg gcacatcagg cgcggggcaa actctttgcc
      181 gttccaaacc ggtttgattt gggatttgca cacgcccaag atagcgactt cgggagcgtt
      241 cacaatcggc gtgaaacctg tgccgccgat gccgcccaag ctggaaatgg taaagcacgc
      301 gccttgcatt tcttgcggtt tgagcttgcc ttcgcgggct tttttggaca attcggtcaa
      361 ttcttggctg atttgtttca agcctttttg atccacgtct ttgatgacgg gaacaaccaa
      421 gccgttcggc gtatcggctg cgaaaccgat gttgaagtag tttttcagca ccaggttgtc
      481 gccgtccaaa gaagcgttga attcggggaa ggctttcagc gcggaaacgg aggctttgat
      541 gatgaacgcc aacggggaca gtttcacgcc ttcgcgttcc cattctttgt tcagctgttt
      601 gcggaattct tccagttcgg tcatatccgc ttcttcgtgt acggtaacgt gaggaatcac
      661 cacccagttg cgggacaggt tttgaccgga gattttctta atgcgggaca attctttaac
      721 ttcgacattg ccgaatttgg agaagtccac tttaggccac ggcagcaagt ccagaccgcc
      781 gcccaaagat gcgccggctg cggcaggttt tgccgcgccg ccctgcatta cggatttcac
      841 aaaggctttg atgtcgtctc ccatgatacg gcctttcaag ccgctacctt tgacttggcc
      901 caaatccacg cccaattcgc gcgccagttt gcgtgcggaa ggaccggcgt gtgctttggc
      961 gaaagcggct tcatcgattt tggcagcggc aggcgaggca gatgtaggca cggcggcggg
     1021 tgcggcagct tgagcggcag gcgcaggagc tgctgcggca gagccggcgg tttccacttc
     1081 gataatggca gagccttcgg acactttgtc gccgactttt aagaatacgg ctttaacgac
     1141 accggcagcg gtacaaggta cgtccattgt cgctttatcg gtttccaaag taatcagcgt
     1201 gtcgtcttcg gcaacggtgt cgccaacttt gatttcaacg gcgattacat ctacatcggt
     1261 atggccgccg atatcgggaa cggctacttg aacggttgca ccgcctgcgg cagcgggtgc
     1321 aggttgtgct tcggcggcag cagccggggc ggcttcggca gcggcggcac cggtttcaac
     1381 ggtcaaaatt acgccgcctt cggagatttt gtcgccgact ttgactttta cttctttcac
     1441 gacaccggcc gcatcggcag gcacatccat cgtggctttg tcggtttcca gtgtaatcag
     1501 ggtgtcgtca acggcgatgg tgtcgcccgc tttaacttct acggcgatga tgtcgacgtt
     1561 ttcgtgaccg ccgatatcgg ggactttgat ttctacgata ctcatttgag ttcctttaat
     1621 ctttttcagt tggatgccgt ctgaaagctg cttcaggcgg catcggtacg gtttgtctgt
     1681 tcaaaccatt cgggcattcg gttttcagac ggcctgatgt cggaatgggg caaacaggcc
     1741 gtctgaaaca tcaatcagcg tttccagcta ggggctgaat cggctttgat gccgtatttc
     1801 tcaatggctt gttgaacggt ttctttgctg actttgcctt gttccgccaa tgcggccaat
     1861 gctgccacgg caacgttgta gcggtccact tcaaagaagc ggcgcaggtt ggcgcggctg
     1921 tcggaacggc cgaaaccgtc agtgcccaag acatggtagt cgttcgggat gtacgcgcgg
     1981 atacggtcgg catagctgcg gatatagtcg gtagcggcga taaccggacc gtcatgacct
     2041 tgcagttggg aagtaacgaa aggcactttt tcagcttcca gcggatgcag gcggttgaag
     2101 cgttcggttt cgatggcgtc gcggtgcagc aggttgaagg acgggcaaga ccagatgtcc
     2161 gcttccacgc cgaagtcggc tttcagcagc tcggcaccgg caatcacttc ttgcagaatc
     2221 gtaccggaac ccatcaattg gactttcttg tcgcctttgc cgccggcttt cagcaggtac
     2281 atacctttga ggatgtcttg ttccgcacct tccggcatat cgggatgggt gtagttctcg
     2341 ttcatcaggg tgatgtagta gaacacgtct tcattattgg catacatacg gcgcagaccg
     2401 tcttgtacga tgacggccac ttcgtattgg aaggtcgggt cataagatac gcagttcgga
     2461 atcaggtcgg cctggatgtg gctgtggccg tcttcgtgtt gcagaccttc gccgttcagc
     2521 gtcgtacggc cggcagtacc gcccagcagg aagccgcgcg cgtgcatatc gcccgccgcc
     2581 caagccaagt cgccgatacg ttggaaaccg aacatggagt agtagatgta gaacggaatc
     2641 atcgcgaagt cgctgttggc gtagctggtc gcagccgcaa tccagtcggc catcgcgccc
     2701 ggttcgttaa taccttcttg caagatttga ccgtcaacgg attctttata gaacatcagt
     2761 tggtctttgt cttgaggcgt gtattgttgg cctttgggat tccaaatacc gtattggcgg
     2821 aacatacctt ccatgccgaa agtacggctt tcgtcgggaa cgataggtac gacgcgtttg
     2881 ccgatttttt tgtctttcag caaagtggac aggatgcgga cgaatgccat cgtggtcgag
     2941 aactcgcgtt caccgctgga tttgagttgt gcgtcgaatg ccgacagctc gggcacttcc
     3001 aatacttcct gcgtcggttt gcgttgcggc aggtagccgc ccaaagcatc gcggcgtgcg
     3061 tgcaggtatt tgtattcttc cgtatcgggg gcaaaagtca ggtaaggcag atcgccgctt
     3121 tcgatttgtt cgtcggtaac cggaatgtca aagcggtcgc ggaattgttt cagggacgct
     3181 ttgtccattt ttttggcttg gtgggcaacg ttctgacctt cgccggatgc gcccataccg
     3241 taacctttaa tggttttcgc caagataacg gtaggtttgc cgtcggcatg gttcgctgcg
     3301 cggtcgtagg cgttgtacac tttttgagga tcgtggccgc cgcggttcaa tgcccagagt
     3361 tgctcatcgg tcatatcggc aaccaatgct ttcagttcgg gcgtattgaa gaagtgttcg
     3421 cgcacatacg cgccgtcttt ggatttgtaa gtttggtagt cgccgtccaa acattcttcc
     3481 atacgttggc gcaggatacc gtctttgtct ttcgccaaga ggcggtccca acggcggccc
     3541 caaatgactt tgacgacatt ccagccggca ccggcaaagt tgccttccaa ttcttggatg
     3601 attttgccgt tgccgcgcac cggaccgtcc aagcgttgca ggttgcagtt gatgacgaaa
     3661 atcaggttgt ccaagccttc gcgtgcagcc agcgcgattg caccttggga ttcgggttcg
     3721 tccatttcgc cgtcgccgca gaaacaccat actttacggc ctttggtttt tgccaagccg
     3781 cgtgattcca agtatttcag gaaacgcgcc tgataaatcg ccatgatggg gcccaagccc
     3841 atggatacgg tcgggaactg ccagaagtcg ggcaagaggt gggggtgtgg ataggaaggc
     3901 agaccgtgtc cgtccacttc ttggcggaag ttgttcagct ggtcttcggt cagacggcct
     3961 tcgacgaatg cgcgtgcata gatgcccggg gcaacgtgac cttggaagaa gaccaaatcg
     4021 ccttcttcac cttcaccttt ggctttccag aagtggttga aaccgacttc gtacatggtg
     4081 gcggcagatt ggaaagatgc gatgtgccca cccagttcca aatctttctt gccggcgcgc
     4141 aatacgatgg cggcggcgtt ccagcgcacg aatgcgcgaa tgcggtgttc gatgttttgg
     4201 tcgcccggaa tgcctttttc gttttcaacc gaaacggtat tcaaatacgg ggtggtcgtg
     4261 ccgtggggca tacgtacgcc tttgtcgcgg cagtatttga gcaggttttc caagaggtat
     4321 tgcgcgcgtt cgccgccttc atattcgagg acggagctta acgcgtccag ccactcttgg
     4381 gtttcgatag ggtcaacatc gtgtaattgg gtggacatag tatctatcct ttatgttgag
     4441 tgtttaatcg gaagggaggg tgtagccaac ccctttattt tcgtttgtga aaaataataa
     4501 gcaaaaacga aaatttctaa atcaaaccga aacaaaataa tttaagttta atgttttcaa
     4561 aaaattataa aagtcttgga agcaagggaa aacttgaaaa aaaatgttcg gtctgctccg
     4621 cacgaaatcc tatcagcaat cgggtctgct ggcaaataac catgctttta aaagcaggtt
     4681 ttatactgaa gttggcgttt ggtaaggaca ggtctttttg attgcgaaaa cattctgttt
     4741 aattttggtt atattttttc ggaattgtgg gatatcgaat cgttatgtct ctgtcggcaa
     4801 ttttcctatt gcagaaaatc tatacacaag ctttgtggac aaccgtgttg ataagccacg
     4861 gcttgtgtta aaaaagtctt acgcaacaaa tgtacgctta ttttgattaa aaattaggca
     4921 aatttaaatc tataaaaatc atgtaattat gttcaatatg atgtttctgg tcatgttttt
     4981 gccgtctgtt atggggtttg tcccgcgttt ggggcagggg gtgaattttg gaggttggtg
     5041 caaaacctgc ttttttcgcg ttacaatctg cacccgattt acttttgaat ctgcctgttt
     5101 ggaaaaatac ttttatgaaa gccagccaat tctttatctc tactttaaaa gaagcccctg
     5161 ccgaagccgc gtttgccagc cacaagctga tgattcgcgc cggtctgatt aaagccaacg
     5221 cgtccggtct ttatacttgg atgccgatgg ggctgcgcgt gttacgcaaa gtcgaaaacg
     5281 tcgtgcgcga ggaaatggcg cgcgcgggca gcgtggagct gctgatgccg gtggtgcagc
     5341 ctgccgaact gtggcaggaa tccggccgct gggagtttta cggtaaagaa ctgctgcgcc
     5401 tgaaagaccg ccacgaacgc gatttctgca tgggcccgac ctgcgaggaa gtcatcgccg
     5461 acatcgtgcg caaagaaatc aacagctaca aacaactgcc gaaaaatttt taccacatcc
     5521 aaaccaaatt ccgcgacgaa gtgcgcccgc gtttcggcgt gatgcgcgcg cgcgaatttg
     5581 tgatgaaaga tgcttattcc ttccacgccg actacgcctc gcttcaggcg acctatgatg
     5641 ccatgtatga cgcttactgc cgcatcttta cccgtctggg tttggcgttc cgccccgtcg
     5701 ccgcagacac cggcagcatc ggcggtacgg gttcgcacga gtttcaagtg ttggcggaaa
     5761 gcggcgaaga tgtgattgca tacagcgaca cttccgatta cgccgccaat atcgagttgg
     5821 caccgacctt gccgcttaaa ggtgaacgtg ccgccgctca ggctgtgttg accaaagtac
     5881 atacaccaaa cgtcaaaacc attgagtctt tggttgaatt cctgaatatt ccggttgaac
     5941 aaaccctcaa atccatcgtg gttgaaggcg aaaacgaagg cgaactcgtc ctactgctgt
     6001 tgcgtggcga ccatgagttt aacgacatca aggcagaaaa actggcgggc gtaaaatcgc
     6061 cactgactat ggcaagccct gccgcgattg ttgaacaatt cggcgcaaac ggcggttcgc
     6121 tcggccccgt cggcttcaca ggcaaagtct atgccgattt cgctaccgaa aaaggcgcgg
     6181 actgggttat cggcgcaaac gaagacgact accactatac cggcttcaac ttcggccgcg
     6241 atgccgccga gcctgaattc gtcgatttgc gtaatgtggt cgaaggcgac gaaagccccg
     6301 acgggcaagg ccgtctgaaa ctggcgcgcg gcatcgaagt cggacatgtc ttccagttgc
     6361 gcggcaaata tacccaagct atgaatgtca gcttcctcga caataacggc aaatcgcaaa
     6421 tcatggaaat gggctgctac ggcatcggca tcacccgcgt cgttgccgcc gccatcgagc
     6481 agaataacga cgaaaaaggc atcatttgga ccaaagcgat ggcgccgttt gaagtcgtca
     6541 tcgtgccgat gaactacaaa aaatccgaca ctgtgcgtga agccgccgac agaatctatg
     6601 ccgaattgct ggcggcaggc gcggatgtgc tgctggacga ccgcgacgaa cgcgcaggcg
     6661 tattgctgaa cgattccgag cttctcggta tcccgcaccg catcgtcatc ggcgaccgcg
     6721 ccttgaaaga aggcaatgtc gaatacgccg aacgccgcga caacgaagcg caggcagttg
     6781 caatcggaga aattgttgcg cgtgtaacag cttcattaaa tgcgtaagtc tggtcaaatg
     6841 ccgtctgaaa ataatttcag acggcatttt tattttgggg gaaagtgaag tggcttcagt
     6901 atttttagaa ggagaagcag tcgaggtcgg caatattttc tgtatcggca ggaactatgc
     6961 cgcacatgtc gaagagttaa aaaacgaaat accttcagaa cctgtggtgt ttatgaagcc
     7021 gtcaggcagc attttgaaca gcggcggtac aatcttgctg cccgagttca gtagggatgt
     7081 gcagtttgag tgcgaactag tgttgctgat tggccgggat tcagacggca cgggcgaggg
     7141 tgaagatatt ttgggatgcg tggcggggta cggcgtgggt ttggatttga ccgcgcggga
     7201 catccaatgc cgtctgaagg cgcaggggct gccctggctg aaggcaaaag ggttcaggca
     7261 ttctgcgtgc gtgtcggatt ttgcggcggc agacagaatc ggcaatcaag ataaggtgtt
     7321 gttttcatta aaacaaaacg gcgtattgaa acagcgcggg gagactggtt tgatgattta
     7381 cccgattcgg gaaattttac acaaactggc ggcggattac ggtttgggga aaggggattt
     7441 ggtgtttacc ggaacgccct ccggcgtggg cgcaatcggt gcgggggaca atttggcatt
     7501 ggagctggac ggcttggtgt gcgcctcttt cacgataggg tgttaaccgc gccgcttaac
     7561 cttcacgccc gttgaaacat tccgccgggc cgccctcgag aaaaccgtgc aggatggctt
     7621 gcgccgccac ttggtcgagc accgatttgc gttttttgcc caagacctgt gcttccgaaa
     7681 gcaggctttc ggcatagacg gacgacagcc gttcgtcaac ccaatagacg gggagattga
     7741 acctgccgtt cagcctgcgt ccaaacttgc gcgacaggtg cgtcatttca tgttccgtgc
     7801 cgtcggcatg cacgggcagt ccgacgacaa aataacgcgg ctgccattct tgaaccagct
     7861 tggcgattgc cgcgaatttt tcatcgttgc tgccgccggt aacggtggcc agcggatggg
     7921 acagccccaa ttccgcatcg ccctgcgcca cgccgatgcg cgcttcgccg aagtcgaatg
     7981 ccagtgccgt tccttttgga attttatgca tggccggctc ccgaaaacag ggcgagcggg
     8041 tcgatgccga gtttggcgaa tgcggcggcg taacggtgtt cgtaggggat gtcgaacagg
     8101 atgtgttcgt cggcgggaac ggtcagccac gcattgtcgg caagttcgcg ttcgagctgc
     8161 cctttgctcc agctcgaata gcctatgctg atcaaggctt tgtcaaccgc gccttcgcgt
     8221 gaaatgtttt caagcacatc gcgggaagag gtcagcgcga tgccgtctga aacgccgatg
     8281 ctgctttgcc agttgccgat cggggtgtgg acgacgtaac cgcgctcgac ctgcaccggc
     8341 ccgcccatca tcacgctgtc gtgctgcatc cgcatgggga tgtttttgcc ggtggcggaa
     8401 aaaatcatgt ccatcgtaat cggagagggt ttgttgatgg cgataccgag tgcgccgtct
     8461 tcatcgtgtt tgcagatata gacgaccgat tgtgaaaaaa acgcgtcttc catatcgggc
     8521 atggcgacga gaaagtggtt ggacaggttc atatcagcgt ttgtcgggat tttttatcgg
     8581 gttggaaata atgcaggcgc cggcaagcag tttgccggaa gtatcggctt gcaccccgtc
     8641 ggtttgcggt atgccgctgc cgaaacagga aaccgcgctt aaggaatttt tcggcgatcc
     8701 ttggacgatt ttatcgctgt aaaccaaata ggcgaaggct ttgcgtttgg ggtcgtaata
     8761 acggacaatc tgccggctct tgaacgcgaa gctcgcgccg cgcttgaaaa cttctttcgg
     8821 tttgcgcacg gcggtttcgt caaaagaaat cgaagatgcc gtctgaacgc acgaaaccga
     8881 tgcgtcggac gcgtcctctt ccaaattgac catttccttc aagccgcctt tttttgcata
     8941 cgaaatataa caggcaaccc cttgaacgtc gggatcgtcg aatccttcca cttcgatacg
     9001 gtcgtttttg cccaacatgt tgaaaacggt acttgcccgt ccgattttat cggtttcgcc
     9061 gctgccgcag gcagtcggca ggaaggcggc agacagcagt agcagtctgt tcatcaaaat
     9121 ttccttatcg gttgaatccc cgttttcagg aaggcggcag ggtttgcttt gtttggaggg
     9181 gcgcacgctc ccaaatcggg caacatacgg tacgcagctt acatgtgcta ccctgtgtgt
     9241 cggaacacaa tatccgttgc agcattgaaa atatgccgca caatataagg ctgtttgagc
     9301 cgattgcaag tatccgcgca aagaaggtgc aagattgggc aaacggcatt ttcagacggc
     9361 ataactgaca gtataatcca aacgtccggc tgctttcgtg cagccttcag tattatccaa
     9421 cttttccgaa ggtcgaacca tgcgctacaa accccttctg cttgccctga tgctcgtttt
     9481 ttccacgccc gccgttgccg cccacgacgc ggcacacaac cgttccgccg aagtgaaaaa
     9541 acaggcgaag aacaaaaaag aacagcccga agcggcggaa ggcaaaaaag aaaaaggcaa
     9601 aaatgccgca gtgaaagata aaaaaacagg cggcaaagag gcggcaaaag agttcaaaaa
     9661 aaccgccaaa aaccgcaaag aagcagagaa ggaggcgaca tccaggcagt ctgcgcgcaa
     9721 aggacgcgaa ggggataagg aatcgaaggc ggaacacaaa aaggcacatg gcaagcccgt
     9781 gtccggatcc aaagaaaaaa acgcaaaaac acagcctgaa aacaaacaag gcaaaaaagg
     9841 ggcaaaagga cagggcaatc cgcgcaaggg cggcaaggcg gaaaaagaca ctgtttctgc
     9901 aaataaaaaa gcccgttccg acaagaacgg caaagcagtg aaacaggaca aaaaacacac
     9961 ggaagagaaa aatgccaaaa ccgattccga cgaattgaaa gccgccgttg ccgctgccac
    10021 caatgatgtc gaaaacaaaa aagccctgct caaacaaagc gaaggaatgc tgcttcatgt
    10081 cagcaattcc ctcaaacagc ttcaggaaga gcgtatccgc caagaacgta tccgccaaga
    10141 gcgtatccgt caggcgcgcg gcaaccttgc ttccgtcaac cgcaaacagc gcgaggcttg
    10201 ggacaaattc caaaaactca ataccgagct gaaccgtttg aaaacggaag tcgccgctac
    10261 gaaagcgcag atttcccgtt tcgtatcggg gaactataaa aacagccggc cgaatgcggt
    10321 tgccctgttc ctgaaaaacg ccgaaccggg tcagaaaaac cgctttttgc gttatacgcg
    10381 ttatgtaaac gcttccaatc gggaagttgt caaggatttg gaaaaacagc agaaggcttt
    10441 ggcggtacaa gagcagaaaa tcaacaatga gcttgcccgt ttgaagaaaa ttcaggcaaa
    10501 cgtgcaatcc ctgctgaaaa aacagggtgt aaccgatgcg gcggaacaga cggaaagccg
    10561 cagacagaat gccaaaatct ccaaagatgc ccgaaaactg ctggaacaga aagggaacga
    10621 gcagcagctg aacaagctct tgagcaattt ggagaagaaa aaggccgaac accgcattca
    10681 ggatgcggaa gcaaaaagaa aattggctga agccagactg gcggcagccg aaaaagccag
    10741 aaaagaagcg gcgcagcaga aggctgaagc gcgacgtgcg gaaatgtcca acctgaccgc
    10801 cgaagacagg aacatccaag cgccttcggt tatgggtatc ggcagtgccg acggtttcag
    10861 ccgcatgcag ggacgtttga aaaaaccggt tgacggtgtg ccgaccggac ttttcgggca
    10921 gaaccggagc ggcggcgatg tttggaaagg cgtgttctat tccactgcgc ctgcaacggt
    10981 tgaaagcatt gcgccgggaa cggtaagcta tgcggacgag ttggacggct acggcaaagt
    11041 ggtcgtgatc gatcacggcg agaactacat cagcatctat gccggtttga gcgaaatttc
    11101 cgccggcaag ggttatacgg tcgcggcagg aagcaaaatc ggcacgagcg ggtcgctgcc
    11161 ggacggggaa gaggggcttt acctgcaaat acgttatcaa ggtcaggtgt tgaacccttc
    11221 gggctggata cgttgatcgg attgccgaaa cggcaggctt tatgcaaatc gtgccgatat
    11281 ggtgcgctgt tctgtgaagt ttgccgtttg ccgtacctgt gttttccggt ttttggaaat
    11341 caagagagaa agttaaagaa gaatgtcgaa acctgttttt aagaaaatcg cactttatac
    11401 tttgggtgca atcagcggcg tggccgtaag tctggcggtg cagggttttg ccgccgagaa
    11461 ggacgggcgg gataacgaag tcctgccggt gcaatccatc cgtacgatgg cggaggttta
    11521 cggtcagatt aaggcaaact actatcatga caaacccgat gccgatttgt ttgaaggtgc
    11581 gatgaagggt atggtggccg gtttggatcc gcattccgaa tatatggata aaaaaggtta
    11641 tgccgagata aaggagtcca ccagcggcga atttggcggc ttggggatgg aaatcgggca
    11701 ggaagacggt tttgtcaaag tggtttcgcc gattgaggac acgcctgccg aacgggcgga
    11761 ggtgaaaagc ggcgatttca ttgtgaaaat cgataatgtt tcgacgcgcg gtatgacggt
    11821 cagcgaagcg gtgaaaaaaa tgcggggcaa gccgggtacg aagattactt tgacgctgtc
    11881 gcgcaaaaat gccgacaagc cgatagtcgt caacctgacc cgtgccatta ttaaagtgaa
    11941 aagcgtccgc catcacctga tcgaacccga ttacggctat atccgcgtgt cgcagttcca
    12001 agagcggacg gtcgaaagcg tcaataccgc cgcaaaagag ctggtaaagg aaaataaagg
    12061 aaaaccgctc aaggggctgg tgttggattt gcgcgacgac cccggcgggc ttttgaccgg
    12121 cgcggtcggc gtgtcggcgg cgtttctgcc gtctgaagcg gtcgtcgtca gcaccaaggg
    12181 acgcgacggc aaagacggca tggtactgaa agccgttccc gaggattatg tgtacggtat
    12241 gggcggcgat cctttggcgg gtattcctgc cgagttgaaa acgattccga tgacggtatt
    12301 ggtcaattcc ggttcggctt ccgcgtcgga gattgtcgcc ggcgcattgc aggaccacaa
    12361 acgcgcggtc atcgtcggta cgcagagctt cggtaaaggt tcggttcaga ctttgattcc
    12421 tttgtccaac ggcagcgcgg tcaagttgac gaccgccctg tattacacgc cgaacgaccg
    12481 ttccattcag gcacagggga ttgttcccga tgtcgaagta aaagataagg aacgtacttt
    12541 tgaaagccgc gaggcggacc tggtcggaca catcggcaat cccttgggcg gcgaggatgt
    12601 gaacagtgaa acccttgccg taccgcttga aaaagatgcg gataagcccg ctgcaaaaga
    12661 aaaaggtaaa aagaaaaagg acgaggattt gtcttcaagg cggattccga accctgcgaa
    12721 agacgatcag ttgcgtaagg ctttggattt ggtcaagtcg cccgagcagt ggcagaagtc
    12781 tttggggctg gcggcgaaaa aaccggtttc aaataaagat aaaaaagata agaagtaggt
    12841 tttgacgatg ccgtctgaaa ggatttcaga cggcatatta tgtttgaaag gacaggttat
    12901 gagcgatttt ttaccggaat ttgaaccgtg cggcatttat agtggattaa caaaaatcag
    12961 gacaaggcga cgaagccgca gacagtacaa atagtacggc aaggcgaggt aacgccggac
    13021 cggtttaaat ttaatccact atacttgggt agcgattggg tttcagacgg catacattca
    13081 ggatttcacc gccttatacc gtctgaaaga cgtttctgac ccatccctac tacacgcttt
    13141 gtccgcaaac gtatcacagg caagattatg gaagttatcc gatacccaaa ttcccccttc
    13201 aaactccacc aacccttccc gcccgcaggc gaccagccca ccgccattgc gggcctgatc
    13261 gaagggcttt cagacggcct tgcctaccaa accctgctcg gcgtaaccgg ttcgggcaaa
    13321 acctacacga tggcgaacgt catcgcgcaa agcggcagac ccgccatcat tatggcgcac
    13381 aacaaaaccc ttgccgccca gctttatgcc gaaatgcgcg agtttttccc cgaaaacgca
    13441 gtggaatatt tcgtgtcgta ttacgactat taccagcccg aagcctatgt gcccagccgc
    13501 gatttgttca tcgaaaaaga cagcgcgatc aacgaacaca tcgagcagat gcgcctttcc
    13561 gccaccaaaa acctgatgac gcgcgacgac gtgattatcg tcgccaccgt gtccgccatt
    13621 tacggtatcg gcgacccgac cgagtatcaa caaatggtgc tgtccgtaaa agaaggcgac
    13681 accatcgagc agcgcgacat catcgccacg ctcgtttcca tgcagtacga acgcggcgat
    13741 ttggacttca aacgcggcag cttccgcgtg cgcggcgacg tgattgacgt gtaccccgcc
    13801 gaaagctccg aaaacgcctt gcgtatcagc ctgtttgatg acgaaatcga ccgcctcgat
    13861 atgttcgacc cgctttcagg cagcctgcac cagcgtgtcg gacgctacac cgtcttcccg
    13921 tccagccact acgttacccc gcgcgacacc gtattgcgcg cctgcgaatc catcaaagaa
    13981 gaattgcgcg aacgcatcga atttttcgct cgcgaacaac gccccgtcga gcaacaacgc
    14041 atcgaacagc gcacccgttt cgacctcgaa atgctctacg aaatgggctt ctgcaaaggc
    14101 attgaaaact actcccgcca cttctccggt aaaaaagaag gcgaaccgcc gcccacgctg
    14161 atggactacc tgcccgacaa cgccatcatg ttcatcgacg aaagccacgt taccgttacc
    14221 caaatcggcg gcatgtacaa aggcgacgca tcgcgcaagc aaaacctcgt ggactacggc
    14281 ttccgcctgc cttccgcccg cgacaaccgc ccgctcaaat tccacgaatt tgaaaaagtc
    14341 atgccgcaaa ccgtcttcgt ttccgccacc cccgcgaaat acgaagaaga acacgccgga
    14401 caagtcgtcg aacaagtcgt ccgccccaca gggctggtcg acccccaaat catcatccgc
    14461 cccgtcgcca cccaagtcga cgatttaatg agcgaaatca acgaccgtat tcaaaaaggc
    14521 gaacgtgtgc tcgttaccac cctcaccaaa cgcatggcgg agcaactcac cgactattac
    14581 agcgaactcg gcatcaaagt gcgctacctg cacagcgaca tcgacaccgt cgagcgcgtt
    14641 gaaatcatta gagatttgcg gctcggcctg tttgacgtac tcgttggcat caacctgttg
    14701 cgcgaaggcc tcgacatccc cgaagtgtcc ctcgtcgcca tcctcgatgc cgacaaagaa
    14761 ggcttcctgc gctcccaccg cagcctgatt caaaccatag gccgcgccgc gcgcaacgtg
    14821 aacggcgtcg ccatcctgta cgccgacaaa attaccgact ccatgaaagc cgccgtcgac
    14881 gaaaccgaac gccgccgcga aaaacagatt aaattcaacg aagaacacgg tatcgtgccg
    14941 cagcagatta aaaaacaggt caaagacatc atcgacggcg tgtaccacga agaagacagt
    15001 ggcaaaggcc gtagacaagg caaaaacaag gtcaaagtcg gcgaaatcca caacgaagaa
    15061 gacgcgatta aagaaatcgc caaactggaa aaagccatgc agcaggcggc tagggattta
    15121 cagtttgaag aagctgctgt gttgagggat aggattagga atattaaaga gaatttgttg
    15181 tttggggcgg agtgattttg aaatagtaat cgcagcctga atacatagcg tgtgcgccac
    15241 tggcgcgcgc tcattgtgtt atcaggaatc cttgaaatca tgtacgcagc ttgcatcaca
    15301 catcctagct atacataaac aacaaggccg tctgaatttt cagacggcct tttccgttta
    15361 caactttact acttattcaa taactacacg tgcattcaga ggctgtacgg ggcggttgtt
    15421 ttccgaaccg acggcgcggg tgaatttttc cgcttgcgct tggtcgatgt ggtcataagt
    15481 tttcaacacc aaccatgata cgccctctgt gcacggcggc gtggtcagcg aaccggcaaa
    15541 gcggtagtat ttcaaccgtt tcggcagtag ggtggatgcg tcgaacggtt ggttgagttt
    15601 cacttttcct gcggtcatcg gcatgacgtt ccagatggaa gacaggcggc cgttggtttt
    15661 gccggcttca tacagcacgg ctaatactaa aggctgtttg ttttcgtcta agtggacgaa
    15721 gtgagcttcc atcgggaaag tgcggccttt gatttggttt tcgctcggca cgtggaagtg
    15781 gaactgtttc agggtatagg tgcggccgtt cacggtcagg gtattgccgc cttcgggata
    15841 attgacctga atggtgtggc cgttgttttc cacgtcaacc atactcggtt tgtaattgac
    15901 tttgatggcg ggcagtttgc cggaaacggt ttcggtaatg tttaccggag attggttttt
    15961 gccggtggag cacaaacgga attcttctga cagattgccc cagctttcgg gagagtcgtg
    16021 tccggtatag ccccaatggg tgtgattgcc gtgtgcggcg gcggagaaag cggtacaggc
    16081 caacagcagg acagcggtca ggcggggtaa agtgcggggg aaacgaggca ttgtaagatc
    16141 ctttctatta aggagtagaa aaaaggttgc agtgtgtatg taaatatttt attttttatt
    16201 aaatgatatc tatattaagt gcttaagtca aacttcccgg tctgaaaacc attagagcaa
    16261 tgaaattaaa gtgaatttat ttttctataa gtcctgcttt atttggataa ccatgaggca
    16321 tggctacctt tatccggaaa aatattaata gccgcaacaa agatatttga atccgattca
    16381 aatatcacca gcaagcgtag ctcggatact tgtatccgac aaagccttta acatttccat
    16441 cgttgcaatc cattgcagca attaccaaaa tgtcggattc gagaatccga tctacggaaa
    16501 actaaagatt ttatgcgggc tacggcttgc ttgttgtatt taatcatatt tatttgaatt
    16561 gaattctttt attttattgt gagatgaaaa tagtattttc gatgagaaac ccatgactaa
    16621 aatagtatag tctttatcat tctttacgaa gtaacaatta ccatatcctg caatattttt
    16681 atcccaaaaa tcacaatatg attcagtggt aaaatcagtt ttagttgata taa
//
LOCUS       DAXFZR010000052        16722 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000052 DAXFZR010000000
VERSION     DAXFZR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16722
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.052"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(225..1154)
                     /locus_tag="ACFJAS_001424"
     CDS             complement(225..1154)
                     /locus_tag="ACFJAS_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC3982655.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1283..2032
                     /locus_tag="ACFJAS_001425"
     CDS             1283..2032
                     /locus_tag="ACFJAS_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC3982656.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            2155..2736
                     /gene="petA"
                     /locus_tag="ACFJAS_001426"
     CDS             2155..2736
                     /gene="petA"
                     /locus_tag="ACFJAS_001426"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC3982657.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2755..4104
                     /locus_tag="ACFJAS_001427"
     CDS             2755..4104
                     /locus_tag="ACFJAS_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC3982658.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIIAFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            4119..4907
                     /locus_tag="ACFJAS_001428"
     CDS             4119..4907
                     /locus_tag="ACFJAS_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC3982659.1"
                     /translation="MKQAMKNWFAALLSAVPMSAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5239..7029
                     /gene="rmuC"
                     /locus_tag="ACFJAS_001429"
     CDS             5239..7029
                     /gene="rmuC"
                     /locus_tag="ACFJAS_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC3982660.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(7096..7728)
                     /locus_tag="ACFJAS_001430"
     CDS             complement(7096..7728)
                     /locus_tag="ACFJAS_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC3982661.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7821..8645
                     /locus_tag="ACFJAS_001431"
     CDS             7821..8645
                     /locus_tag="ACFJAS_001431"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC3982662.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8714..9277
                     /locus_tag="ACFJAS_001432"
     CDS             8714..9277
                     /locus_tag="ACFJAS_001432"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC3982663.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKEKPVKADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9360..9797
                     /locus_tag="ACFJAS_001433"
     CDS             9360..9797
                     /locus_tag="ACFJAS_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC3982664.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDFAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            10018..10287
                     /locus_tag="ACFJAS_001434"
     CDS             10018..10287
                     /locus_tag="ACFJAS_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC3982665.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            10287..12062
                     /gene="ptsP"
                     /locus_tag="ACFJAS_001435"
     CDS             10287..12062
                     /gene="ptsP"
                     /locus_tag="ACFJAS_001435"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC3982666.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(12189..13130)
                     /locus_tag="ACFJAS_001436"
     CDS             complement(12189..13130)
                     /locus_tag="ACFJAS_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3982667.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(13139..14014)
                     /locus_tag="ACFJAS_001437"
     CDS             complement(13139..14014)
                     /locus_tag="ACFJAS_001437"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC3982668.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(14197..14273)
                     /locus_tag="ACFJAS_001438"
     tRNA            complement(14197..14273)
                     /locus_tag="ACFJAS_001438"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14237..14239),aa:Met,seq:cat)
     regulatory      14422..14521
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            14612..16513
                     /gene="thiC"
                     /locus_tag="ACFJAS_001439"
     CDS             14612..16513
                     /gene="thiC"
                     /locus_tag="ACFJAS_001439"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC3982669.1"
                     /translation="MTTPKKTAKISGNEARELSDLSEDIGIRFKYQNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         4017 a         4368 c         4659 g         3678 t
ORIGIN      
        1 atggagttat aaggatattt cgttaatctc attattttgc tccatgtcta aaacgtcctg
       61 ttgatccata tatttcaccc gttatgagtc tgttcccatc tcatatagtt tcaagtttaa
      121 caaccccttg aggctatgtt aaaagagttt ttctaataac tattccgttt tcttaaatat
      181 aaaaaaatag aaaaatagcc cgggtatagt ttgaacaagg gaaatcagac cggttccaac
      241 tcacttaatc cgggcaagtc cgcgaagccg cgttcacgta tgatttggca aaagttgttc
      301 agataacttt tatctgtgtc ttcagtgcgg atggcggcat acagtttgct ttgcagtccg
      361 tcggcagtaa tttggcggtg gacgacatag cctttttcaa ggtagggcat gactgtccaa
      421 tagggaaggg cggcaatgcc acgtctgctg gcaaccagtt ggataatggc gatggtcagc
      481 tcgctgtgcc ggcgcggcgg gttgatgttt ttcggaatca ggattttttt gggtaaatcc
      541 agcatctcgt cgggaacggg ataggtaatc agggtttccc cgataaagtc ttccgccgtc
      601 caaacgtttt tggcggcaag cggatggtcc ggggcgcaaa tgccgaccat ttcgtaggca
      661 aacagtgggt ggaagctgat gctgctttgt ttttcggctt cggagacaat ggcgaggtcg
      721 gcgcggtgtt gcagcagcag cccgacggga tccgcttgga atcctgatac gatatccagt
      781 tcgacttggg gccacatcgg gcggaattcg cccatggcgg gcatcagcca gtcgaaacag
      841 gtatggcatt cgacggcaat ccgcagctct cccgcctccc cttccgtgat tcgcgccaaa
      901 tctcgttctg caacagcaac ttgaggtatc agttcgtggg cgaggcgcag cagcctttcg
      961 cccaccgggg taaagcgcag gggcgtggat ttgcgttcga acagcggcgt gccgtagtgg
     1021 ttttcgagca tacggatctg gtgggaaagg gcggattggg taaggaaaac ccgtttggcg
     1081 gcaagggaga cgctgccggt ttcttcaagt gccagcaggg ttttgaggtg gcgcaattcg
     1141 ataatggaat ccatggggct tcagacggca tattgaaccg gcgcatatta aaataattca
     1201 tatgcgggtg caaatcggaa aaatgggcgt ttcgggcatt tgcggttaga atgcccgctt
     1261 gtttggaaat gacaggaaaa atatggtttt gcgcagagat tttttagcct ggtgcgacga
     1321 aacgttgcag acggcatcgt ttaaagatta cgcccccaat ggtttgcagg ttgaagggag
     1381 ggaatatatc gggaaaatcg ttacgtcggt aacggcaagc agggcagcga ttgattttgc
     1441 tgtggagcag aaggcagatt tgcttttggt acatcacggt atgttctgga aaaacgagct
     1501 gccgaccgtt accggttgga aaaaagaacg gattgccgca ctgttacggc acgacatcaa
     1561 tatggcaggc taccatctgc ccctggatgt gcatcccata ctgggcaaca atgcccaact
     1621 cgccgacaga ttgggttttg cgacagaaaa acggttcggc gaacaaaacc tgctcaactc
     1681 gggcagcctg aaacaagcca agacactcgg cgcattggcg gcgcatattg aaacagtttt
     1741 gcaacgtaag cctgtcgcca tcggcaatcc cgaacgcgaa atccgacggg ttgcatggtg
     1801 taccggcggt gcacagggct tttttcagac ggcaatagat gaaggcgtcg atctgtatct
     1861 gacgggtgaa atttccgaag cccaatacca cctcgccaat gaaacgggta cggctttcat
     1921 ttcggcaggg catcacgcga cggaacgtta cggcgtacgc gcgctggcag aatcggcggc
     1981 agaggttttc gggttggaag tgtgccattt tgacgaaaac aacccggctt gagtcttgag
     2041 aaatatcata aaactttacc ttattttaaa taatgctttg agtatcactg caaactgggt
     2101 aaaattgcaa tgtttaatgg ctcggcattc ccaaaatatt aggacgactg aataatggat
     2161 aatcaagaaa tcaacaacgg ccgccgccgt ttcctgacac ttgcgacctg cggcgcgggc
     2221 ggagtggcag cattgggtgt ggcaacgccg tttgtggcca gttttttccc ttcggaaaaa
     2281 gccaaggccg ccggtgctgc cgtcgaggtg gatgtcagta aaatcgaagc gggtcagctg
     2341 ctgaccgccg agtggcaagg caaaccgatt tgggtgctca accgtacaga tcagcagctt
     2401 aaagacctga aaggcctgaa cggcgaactt accgatccca attccgatgc ggaacagcag
     2461 ccggagtatg ctaaaaacga gacccgttcg attaagccga acatccttgt cgccatcggt
     2521 atctgcaccc atttgggctg ctcgcccacc ttccgtcccg acattgcccc cgccgatttg
     2581 ggcgcagact ggaagggcgg cttcttctgc ccgtgccacg gttcgaaatt cgacttggcc
     2641 ggccgcgtat ataaaggtgt tcctgccccg accaacctgg ttgtcccgcc atataaatac
     2701 ttgagcgaca caactatctt ggtgggcgaa gactaagaat aaggaacgat aattatggca
     2761 aaccaaacca atagcaaagc aaaagcattg ttaggctgga tggacgctcg cttccccctg
     2821 tctaaaatgt ggaatgagca tttggcgcaa tactatgcgc cgaaaaactt taatttttgg
     2881 tactatttcg gctctttagc cctgctcgtc ctcgtgattc aaatcgtcag cggtattttc
     2941 ctgacgatga actacaaacc ggacggcaac ctgaatgcct accatctgcc tgctgccttt
     3001 accgcagtag agtacatcat gcgcgacgtg tccggcggtt ggattatccg ctacatgcat
     3061 tccaccggcg catctttctt cttcattgtt gtttatctgc acatgttccg tggtttgatt
     3121 tacggttcgt acaaaaaacc ccgtgagttg gtgtggattt tcggttcttt gatcttcttg
     3181 gcattgatgg cggaagcctt tatgggctac ctgctgcctt ggggtcagat gtccttttgg
     3241 ggtgcgcagg tgattatcaa cctgttctcc gccattcccg tcatcggtcc tgatttgtcc
     3301 acttggattc gcggcgactt caacgtttcc gacgttaccc tgaaccgctt cttcgccctg
     3361 cacgttatcg ccgtaccgct ggtattgctc ggtttagtcg tggcacacat catcgcactg
     3421 cacgaagtgg gctctaacaa ccccgacggt gtggaaatca agaaaaacaa agatgaaaac
     3481 ggcattccgc gcgacggcat cccgttccac ccttactaca ctgttaaaga catcctgggc
     3541 gtcgttgtat tcctgattgt cttctgtgcc gtattgttct ttgcccctga aggcggcggc
     3601 tacttcctgg aagcgccgaa cttcgatgcg gcaaatgcgc tgaaaacacc cccgcacatt
     3661 gcgccggtat ggtatttcac accgttctac gccattttgc gtgcgattcc ttccttcttg
     3721 ggaacgcagg tttggggtgt aatcggtatg ggcgcggcgg tcgtgttgat tgccctgctg
     3781 ccttggctgg acaggggcga ggtgaaatcc gtccgttacc gcggcccgat tttcaaaacc
     3841 gcattggtct tgttcatcat tgcctttatc ggtttgggta ttttgggcgc gatggtggca
     3901 accgatacgc gtactttggt cgcgcgcatc ctgtccttcg tctatttcgc attcttcctg
     3961 ggtatgccgt tctataccaa gctggataaa aacaaaccgg ttcccgaacg cgtaaccatg
     4021 agcaccgcca aacaaaaaat catgttcttt gtttacgtcg gcattactgc cgtgggtgct
     4081 tacctgtttg caaccaacat ctgatgaggg cagtcgaaat gaaacaagct atgaagaatt
     4141 ggtttgctgc cttgctgtcg gcagtaccca tgagtgcggc atttgcttcg ggcggtcatg
     4201 cacactatga gaaagtcgat atcgatttgc gcgaccaagt aagcttgcag cgcggcgcac
     4261 aaatctttac caactactgt ttgtcctgcc actcggcaag cggtatgcgt ttcaaccgtt
     4321 tgaaagacat cggtctgact gatgaagaaa tcaagaaaaa cctgatgttt accaccgata
     4381 atgtcggcga tgtcatgcat tcggcgatga acccgaaaga tgcggcaaaa tggtttggtg
     4441 ctgctccgcc cgatttgaca ttgattgcgc gttccaaagg tgcagactac ctttacgctt
     4501 atatgcgcgg cttctataaa gatccgaccc gtccgagcgg ctggaacaat actgtattcg
     4561 ataaagtcgg tatgccccac ccgttgtggg aacagcaagg cgttcaagcc gttgagttgg
     4621 atgccaaagg tcagccggtt atggtaaaag acgaacacgg cgagatgaag cctaagctgt
     4681 attgggaatc taccggtttg cacagccgcc gtctgcctaa cggcaaagtg atccagaaag
     4741 agtacgacgc atatgtacgc gatttggtca attaccttgt gtacatgggc gaacctgcac
     4801 aactgcaacg caaacgtata ggctatgtcg tgatgatttt cctatttgcg gttatgctgc
     4861 ctttggcgta tttcctgaaa aaagaatatt ggaaagacgt acactaagcg tttggaacaa
     4921 aagggcaaat cctttagggt ttgccctttt ttcattttgc ctgccgtttg aaaagcctga
     4981 atccgtatgc cgtctgaaca tagctgcaac atttcagacg gcatcattct aaaaatgtca
     5041 ggcatcggga acttaatcag ggtttgtggc agattgatat tgaattagga aaaggtctga
     5101 aaccgcaagc agcatggtaa ggcgatgtcg aacaggttta aagccaatcc gctatatttt
     5161 ttggagctgc accgtgcaaa caggtagaat ggcggcggtt ttgtccggtg tgaaaatgtt
     5221 tgaatattag gatgattgat ggagctgatg actgttttgc tgcctttggc ggcgttggtg
     5281 tcgggcgtgt tgtttacatg gttgctgatg aagggcaggt ttcagggcga gtttgccggt
     5341 ttgaacgcgc aattggcgga aaaggcggca agatgtgatt ttattgaaca ggcacacgca
     5401 gaaattgcat cagaattggc cgctttggat gggaaatacg agcatttgca ggacgaaaat
     5461 tatgctttgg gcaaccgttt ttccgcagcc gaaaagcaga ttgcccattt gcaggaaaaa
     5521 gaggcggaat cggttcggtt gaagcagtcg tatatcgatt tgcaggaaaa ggcgcagggt
     5581 ttggcggttg aaaacgaacg tttggcaacg cagctcgggc aggaacggaa ggcgtttgcc
     5641 gagcaatatg cgctggaacg ccaaatccgc caaagagtcg aaaccgattt ggaagaaagc
     5701 cgccaaactg tccgcgacgt gcaaaacgac ctttccgatg tcggcaaccg ttttgccgca
     5761 gccgaaaaac agattgccta tttgcaggaa aaagaggcgg aagcggagcg gttgaggcag
     5821 tcgcataccg agttgcagga aaaggcgcag ggtttggcgg ttgaaaacga acgtttggca
     5881 acgcaaatcg aacaggaacg ccttgcttct gaagaaaagc tgtccttgct gggcgaggcg
     5941 cgcaaaagct tgagcgatca gtttcaaaat cttgccaaca cgattttgga agaaaaaagc
     6001 cgccgtttta ccgagcagaa ccgcgagcag ctccatcagg ttttgaaccc gttgaacgaa
     6061 cgcatccacg gtttcggcga gttggtcagg caaacttatg ataaagaatc gcgcgagcgg
     6121 ctgactttgg aaaacgaatt gaaacggctc caagggttga atgcgcagct gcacagcgag
     6181 gcaaaggccc tgaccaacgc gctgaccggc acgcagaaca aggttcaggg caattggggc
     6241 gagatgattc tggaaacggt tttggaaaat tccggccttc agaaagggcg ggaatatgtg
     6301 gttcaggcgg catccgtccg aaaagaggaa gacggcggca cgcgccgcct ccagcccgac
     6361 gttttggtca acctgcccga caacaagcag attgtcattg attccaaggt ttcgctgacg
     6421 gcttatgtgc gctacacgca ggcggcggat gcggatgagg cggcacgcga actggcggcg
     6481 tatatcgcca gcatacgcgc gcatatgaaa ggcttgtcgc tgaaggatta caccgatttg
     6541 gaaggtgtga acacattgga tttcgtcttt atgtttatcc ctgtcgaacc ggcctacctg
     6601 ctggcgttgc agaatgacgc gggcttgttc caagagtgtt tcgacaaacg gattatgctg
     6661 gtcggcccca gtacgctgct ggcgactttg cggacggtgg cgaatatttg gcgcaacgag
     6721 cagcaaaacc aaaacgcgct ggcgattgcg gacgaaggcg gcaagctgta cgacaagttt
     6781 gtcggcttcg tacagacgct cgaaagcgtc ggcaaaggca tcgatcaggc gcaaaacagc
     6841 tttcagacgg cattcaagca acttgccgag ggacgcggga atctggtcgg acgcgccgag
     6901 aaactgcgtc tgttgggcgt gaaggcaggc aaacagcttc aacgggattt ggtcgagcgt
     6961 gccaacgaac agacggcatt tgctttgggc aaaccttcgg aacaggaagc ggctgacgaa
     7021 gccgaatagg aaaggcggat gcgtttcaga cggcatcagt gcagatgatg ccgtctgaag
     7081 actttgggaa gtcggttaga aaaacggatt gtgccgtttt tcgtgtccga tggaagtcat
     7141 acgcccgtgt ccggcgacaa cttgcacggt ttcggggaag gcgaataatt tgttgcggat
     7201 attgttgatt aagtcggcgt ggttgccgcg cggaaaatcg gtcctgccta tggtttcgta
     7261 aaacagcacg tcgcccgcaa tcagcagctc cgcttcggca caatagaaaa ccacgtgtcc
     7321 gggcgtgtgc cccggaatat gcagcacttg gaaggcatag cgtccgaccg tgagcgtttc
     7381 gccttcttcg agccaacggt tcggcgcaaa ggcgggcgag acggggaatc cgtattgcgc
     7441 ggtggtttgc ggcagcgatt ggagcaggaa ttcatcttcg cggtgcgggc cgaggacggg
     7501 gactttatgc gtttccagca tctcgaccac gccgcccgcg tgatcgagat gaccgtgcgt
     7561 cagccagatt gccgtgagcg tgagtttgcg gtttgccaac gcttgcagca ggaacggcac
     7621 gtcgccgccg acatcggtca ggacggcttc gccgctttcg tcgtcccaaa tcagggtgca
     7681 gttttggcgg aagggggtaa cggggagaat ttcgtaacgt agggtcatcg gcgtgttcct
     7741 aaaacggttt ttcagacggc atcgggtttg ccgtttgttt tatggcggtt tgccgcctgt
     7801 tttgatattg ggggaatcag gtgattaaga agataatcgg cggcatcata ccgattttta
     7861 cggcggtttt catccctgca tcggcaggcg cggcggattt gatgctggcg caggaataca
     7921 aagggcagga cattgccggc tgggcgatga gcgagaagct cgacggcgtg cgcgcgtatt
     7981 gggacggaaa gcacctgata agccgtcagg gttatgcgtt tacgcccccc aaaggcttta
     8041 ccgcgcagtt tccgccttat cctttggacg gcgaattgta tagcggacgt ggtcagttcg
     8101 agcagatttc cgctaccgtg cgttctgttt cttcagactg gcgcggcatc cgcctgcacg
     8161 ttttcgatgt acccaaggcg cagggcaacc tctaccaacg tttggcagtc gcaacgcagt
     8221 ggctgaaaac gcatccgaac gcgccgatta ccatcatccc gcaaatcaaa gtgcgcgacc
     8281 ggcggcacgc gatggacttt ttaaaacaaa tcgaagcgca gggcggcgaa ggcgtgatgc
     8341 tgcgccagcc cgaatcccgt tacagcggcg gcaggagcag ccaattattg aagctgaaaa
     8401 gccaatacga cgacgaatgc acggtaacgc ggcactatga gggcaaaggg cgaaacgccg
     8461 gacggctggg cgcggtcggc tgcaaaaacc gacacggcga attccgcatc ggcagcggtt
     8521 tcaaggacaa agaccgcgac aacccgccca aaatcggcac gctgattacc taccgttacc
     8581 gtggctttac gcggaaaggc acgccgaaat ttgccacatt tgtgcgcgtg cgtaccgacc
     8641 gctgagcagg acagttcaga cggcatccga tactttggtt tataattttc cttttaccga
     8701 ccgattccga catatgaccg atttagaaac caaacgcctt gaaacacagg cgatgcttga
     8761 aaacgccgat cttttgttcg accaaggcca atgccgtgcc gcactgcaaa aagtggcgga
     8821 cgagattacg cgcgatttgg gggataaata tccgttgctc ctgcccgtga tgggcggggc
     8881 ggtggtgttt acggggcagt tgctgccgct gttgcgtttt cccttagatt ttgattatgt
     8941 tcacgtttcc cgttacggcg acaagctgga gggcggcgcg ttcaactgga agcgtatgcc
     9001 cgatgcggaa caaatccggg gcaggcacgt ggtcgtgttg gacgatattt tggacgaagg
     9061 gcatacgatg tccgccattc aagccaaact tttggaaatg ggtgcggcaa gctgccgtgc
     9121 ggcggtgttc gccaataaat taatcgacaa agaaaaaccg gtcaaagccg attatgtcgg
     9181 actggatgtg ccgaaccgtt atgttttcgg ttacggcatg gatgcggcgg gctgctggcg
     9241 taatttgggc gagatttacg cattggacgg aaaataagga cgcgatgccg tctgaaggcc
     9301 tttcagacgg catcgcgccc atacgccggc aggattaatg aggaacagga cgcatcaata
     9361 tgatagggct tttaatcatc acacacgaaa ccataggcga agcctaccgc aagctggcgc
     9421 atcatttttt tccgggcggg ctgcctgaaa acgtccgcat actcggcgtg cagccgacgg
     9481 aaggccaaga cgacattatc aacaacgcca tttccgcgct gcaagagttt cctgaaaacc
     9541 acggtgtatt gattatgacc gacatcttcg gcgcgacccc ctgcaatgcc gcccgccggc
     9601 tcgtgcgcga aaacaaatcg gcgattttga ccgggctgaa cgcgccgatg atgattaagg
     9661 ccgtccaata ttcgccggcg gcggaagact ttgccgcctt taccgaatgc gtcagggagg
     9721 cggcggtaaa aggcattttc gccatcacgt ccgcgcccga agatttggtg tgccggcgca
     9781 gcggcgatgc cgtctgaaga agcggcaggg caggaaaaca ttttaaacgg cgtctgctgc
     9841 cgatatatag cggattaaca aaaatcagga caaggcggcg ggccgcagac agtacaaatg
     9901 gtacggaacc ggttcgcctg gtgcttcagc accttaggga atcgttccct ttgagccaag
     9961 gcggggcaac gccgtactgg tttttgttaa tccactatac ataacacggg aaccgaaatg
    10021 ctcaaacaat ccatcgaaat catcaacaaa ctcggactcc acgcccgcgc gtccagcaag
    10081 ttcacccaaa ccgcgtccca attcaaaagc gaagtctggg ttacgaaaaa cggcagccgc
    10141 gtcaacggca aaagcattat ggggctgatg atgctcgcag ccgccaaggg tacggtcatc
    10201 gaactggaaa ccgacggctt ggacgaagcc gcagccatga aggcattgac cgacttaatc
    10261 aacgactact tcggcgaggg cgaataatga gtatcgtgct gcacggcgtg gcggcgggca
    10321 aaggcattgc cgtcggttgc gcccacctga ttgcgcgcgg tacggaggaa gtgccgcagt
    10381 atgatgttgc tcaggcggac accgatgccg aagccgaacg tttcgatgcc gccgtcaaag
    10441 ccacgcgcaa agagttggaa cagctccgca gcgcgattcc cgaaaacgcc ccgaccgagt
    10501 tgggcgcgtt catctcgctg cacctgatgc tcttgaccga tgttaccttg tcgcgcgaac
    10561 ccgtcgatat tttaagggaa caaaaaatca acgccgagtg ggcattgaag cagcagagcg
    10621 acaaactcgc cgcccaattc gacaatatgg acgatgccta tttgcgcgaa cgcaagcagg
    10681 atatgctgca agtcgtccgc cgcatccaca acaacctgat cgggcagggc aacgagttgg
    10741 aggttgccga caacctgttt gacgaaaccg ttctgattgc aaacgacctt tcacccgccg
    10801 acacggtttt gtttaaagag cagcgcattg ccgccttcgt taccgatgcc ggcggcccca
    10861 ccgggcatac ggcgattttg ggcaggagct tggacatccc gtccgtcgtc gggctgcaca
    10921 acgcgcgcaa actgattacc gaaggcgaaa cggtcatcgt ggacggcatc aacggcgtgt
    10981 tgattatttc gccggatgag tcggtgttga acgaataccg ccgccgcgcc cgcgaatacc
    11041 gcagccacaa acgcgatttg aacaagctca aaaaaaccgc cgccgctacc gccgacgggg
    11101 tctgcatcga gcttgtgggc aatatagaat ccgccgaaga cgtgaaaccg ctgcacaacc
    11161 tcggcgcaga cggcatcggg ctgttccgca gcgagtttct ttacctgaac cgcgatacga
    11221 tgccgtctga agacgagcag tacgaagtgt acagcgcgat tgtcaaaaaa atgaagggca
    11281 aaagcgtaac gatacggaca gtcgatttgg gtgtggacaa aaatccgcgc tggttcggga
    11341 aaaacagcac gcccaacggc agcctcaacc ccgcgctggg catgaccggc atccgcctgt
    11401 gccttgccga accggtcatg ttccgcacgc agatgcgcgc catcctccgt gcggcggtac
    11461 acggccccgt ccgcatgatg tggccgatga ttacctccgt atccgaagtg cgccagtgcc
    11521 tcatccacct cgacaccgcg caacgccagc ttgccgaacg cggcgatgcc ttcggcgaag
    11581 tcggcatcgg ctgcatgatt gaaattccgt ctgccgcgct gaccgtcggc agtattttga
    11641 aactggtcga tttcatctcc gtcggtacca acgatttgat ccaatatatt ctgtccgtcg
    11701 atcgcggcga cgacagcgtc agccatctct accaacccgg acatccttcc gtgctgaaaa
    11761 tgctgcaaca cgtcatccgt accgccaacc gcatggacaa agacgtgtcc gtatgcggcg
    11821 agatggcggg cgataccgcg tttacccgcg ttttattggg tatggggctg cgccgttttt
    11881 ccatgaatcc gaacaacatc ctgcccgtca aaaacatcat tctgcacagc aatgccggac
    11941 agctcgaagg cgatattgcg aaagtcatcc gctgcgaaga cgaagagaag tcggaaaagc
    12001 tgatcaaaca gatcaacagc gtgtctgtcg cggaagaagc cgaagtcaag ggacggaaat
    12061 aaatattgtt gacaatgcga gcgggttgca gtcaaataac gcccttaaaa aatacagctg
    12121 ctttggtcaa gaaatgccgt ctgaaaaatt ttcagacggc attttgtttg gcaggcattt
    12181 ctgccgcatc atcggaaacg gacgatacgc ccttcatcga cgcggatgcg gaccgtaccg
    12241 tttccggaaa taccgtccgt atgttgtccg acagtcaggt ttaaggtcag ctcgccgtgt
    12301 tcgggatgga cggcggaaag ccggagcgag tcgggcaggc ggacgaggga cagcaggcgg
    12361 cattccgttc catgattgtc caagcacacg gcattttgcg gaatatggcg gtcatcgtcg
    12421 gtattgggca gccccatcag acgggcgacc tgcacgccgg caggcgtttg aatcaaggtt
    12481 tcgggcgtac cgcattgaag gattttcccc tcgtgcatga cggcgatttc gtccgccgcc
    12541 gtgcaggcct cttcgggcga atgcgttacc aaaacggcag ggatgccgcc cttgcggatg
    12601 cgttcggcgg tcatacggcg cagccggtcg cgcaaatgcg tgtccaaact ggaaaacgat
    12661 tcatccagca acagcaggga agggcggaca accaaagcgc gcgccagtgc caaccgttgc
    12721 ttctcgcctc cggaaagttt ttcaggcttg cggtgcgcct cgttttccag cccgacttcg
    12781 gcaagtgccg acaaggcgag gcgttcggct tcggctttcg gcattttttg cattttcaaa
    12841 ccgaatgccg tattttccag cgcactcata tggggaaaca gcgcgtaatc ttgaaacatc
    12901 agcgagatac ggcgtttttc gggcggcata caggtaatgt tttccccgtt cagccgaatt
    12961 tcgccgccgt ccggccggac gatgcccgca atcatattca gcagggtgga tttgccgcag
    13021 cccgaccgcc ccagtacggc gagtattttg ccgcgcccga cagtcaggca gatgttgtcg
    13081 gcgaccgttt tgccgccgaa gcatttgcag agtccgttca gttcaagcat ggcgcatcct
    13141 ataaacgtat gccgtccagc cactcggaca gcggatgggc ggcatcaatc agaccgtaac
    13201 ggtaaaacgc gtccagcgta accagttgcg cgtcgtgcat catgtttttc gacaacatgg
    13261 catccaatag gccgccaatg tccatctttt caaaacccgc tacctcgcca tcctgatttt
    13321 caggcaggaa ggtttcgggc aggacggcat cgaacacata caggatttca ttgtgcacac
    13381 ctcggctgac ggggcgaagg ctgtgcagcc gcgatactgg gcggatgagc ggaaacagcg
    13441 ttttatccaa accggcttct tcgctgcttt cgcggcacac ggcttcagac ggcatttcgc
    13501 cgccggaaac accgccgccg gcaatattgt cgagcttgcc gggatcgact gctttgtgcg
    13561 gactgcgcct gcctatccaa aaatgccatc tgccgttcga ttcgaccaaa ccgttgagat
    13621 ggacggcgcg gctgagtagt ccgaacggac ggaaagcggc gcgttcgagc gtgaacaagg
    13681 ggttgccgcc gccgtcggtc aggtcgaaac actcgttgcg ccatccgtga agcagccccg
    13741 ccttgttcca tgtgcgggcg aggtgctgca agcgtccgcc catatccggc cagccgtccg
    13801 cattcagaaa aatgccgtct gaagactcgg agcagcctgc ctcccagtct tttttgatgc
    13861 gctcagccca ttccggcgac agattgccca aaggaagacg gttcagatac agcgttttcc
    13921 agcaactttc ggcaccgtaa cttgcttttg cccgctcgaa cagggcatca aggtcttgtt
    13981 tgctgacgga ttcggtaaaa cggacggtcg gcatagccgg ctttcttgaa cacaatgcgg
    14041 aagattgtag ccaaaccgcc tgaatttgca accgcaaaca gacggcggcc tgccgttccg
    14101 atattgcggc aggaaggaaa atccggcaaa aaacggaggc ggcaggaaac aggaaaaaca
    14161 aaaccccctc ggcattgaac cgaggggttt gatatttggt tgcgggagca ggattcgaac
    14221 ctacgacctt cgggttatga gcccgacgag ctaccatgct gctccatccc gcgtcagaag
    14281 atgaaactat acggcagatt ttttatgttg tcaaacatta tttccgtgaa aaatcataaa
    14341 gttttccgtc cggcggctta tgcctgattt gaaatatcat tttctttaca aaagttcatg
    14401 ttcgtgattt aattttggtt aacatcgaaa caggggtgct gcctgatgtt caggcggctg
    14461 agaaataccc tttacacccg atcgggataa tacctgcgtg gggagttttc acggattctg
    14521 tctttcagac ggcattggtt ttcaaatgcc gtctgaaaac gcaaaacgct cctgtttctt
    14581 tattctaaac gagaaaacag gagcattttt tatgactacg ccaaaaaaaa ccgccaaaat
    14641 ttccggcaac gaagcgcgcg aactttccga cttgagcgaa gacatcggca tccgcttcaa
    14701 atatcagaac tccgaacgcg tgtatctgca aggcagccgc gacgacatcc gcgtaccttt
    14761 gcgcgaaatc cgtcaggacg acacctacac ggcgcaaggt acggaagcca acccgcctat
    14821 tccggtgtac gacaccagcg gcgcatacgg cgatccggcg gcgcacatcg acctgaaaca
    14881 aggcctgccg cacatccgca cggcgtggct ggacgaacgc ggcgataccg aaatcctgcc
    14941 caagctctcc agcgaatacg gcatcgaacg tgcgcacgat ccgaaaaccg cccatctgcg
    15001 cttcaaccaa atcacccgcc cgcgccgtgc caaagccggc cgcaatgtaa cccagcttca
    15061 ctacgcgcgc caaggcatta tcacgcccga aatggagttc gccgccatac gcgaacgcat
    15121 gaagctggac gagcttttca gacggcctga atacgccaag ctcttgaaac agcacacagg
    15181 gcaaagtttc ggtgcgaaca tcccgacccg tcccgaccaa atcacccccg aattcgtgcg
    15241 ccaagaaatc gccgccggcc gcgcgatcat ccccgccaac atcaaccacc ccgaactcga
    15301 accgatgatt atcggccgca acttccgcgt caaaatcaac ggcaacttgg gcaattccgc
    15361 cgtaacctcc agcctgaccg aagaagtcga aaaaatggtg tggtcgctgc gttggggcgc
    15421 ggacacgatt atggacttat ccacaggcgc gcacatccac gaaacgcgcg aatggattat
    15481 ccgcaacgcg cccgtcccca tcggcacagt gccgatttac caaactttgg aaaaaaccgg
    15541 cggcatcgcc gaagatttga cttgggattt gttccgcgac accttaatcg agcaggcgga
    15601 acaaggcgtg gactatttca ccatacacgc gggcgtgttg ctgcgttatg tgccgatgac
    15661 cgccaaccgc ctcaccggca tcgtatcgcg cggcggttcg attatggcga aatggtgttt
    15721 ggcacatcat cgggaaaact tcctctacac gcatttcgac gaaatctgcg aaatcatgaa
    15781 ggcgtatgac gtatcgttca gcttgggcga cggcctgcgc cccggctgca ttgccgatgc
    15841 caacgacgaa tcccaattcg ccgaattgca caccttgggc gaattgaccg ataaagcgtg
    15901 gaaacacgac gtacaagtca tgatcgaagg ccccggccat gtgccgctgc aacgcgtcaa
    15961 agaaaatatg accgaagagt tgcaacactg ctttgaagca ccgttttaca cactcggccc
    16021 gctcgttacc gacatcgccc ccggctacga ccacatcact tcgggcatag gcgcggccaa
    16081 tatcggctgg tacggcacgg cgatgctttg ttacgttacc cccaaagaac atttggggct
    16141 gcccgacaaa gaagacgtgc gcaccggcat catcacctac aaactcgccg cccacgccgc
    16201 cgacctcgcc aaaggctggc cgggcgcgca attacgcgac aacgccctga gcaaggcgcg
    16261 tttcgaattc cgctggcgcg accaattccg cttaagcctc gaccccgaac gcgccgagag
    16321 cttccacgac gaaaccctgc ccgccgaagg cgcgaaaatc gcccattttt gctcaatgtg
    16381 cggccccaaa ttttgctcga tgaaaatcac gcaggaagtg cgcgactacg ccgacaagca
    16441 aaaagcccaa cggcagggta tggaggaaaa agcggtcgag ttcgtcaaaa aaggcgcgaa
    16501 gatttacagt tgaaacgtca agcaaaaaat gccgtctgaa gcccggaaaa aaggcttcgg
    16561 acggcattct ttcgcttgtg aaaatgatta aacaaccgat cattaaagta atttttaaaa
    16621 tttttatatt cacatagatg ggaatgatgg gatttagggt tctgattttg ttttgagaga
    16681 atgaaggaat ttgagattgt gggcgattat cgggaaaaat ag
//
LOCUS       DAXFZR010000053        16647 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000053 DAXFZR010000000
VERSION     DAXFZR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16647
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.053"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..658)
                     /locus_tag="ACFJAS_001440"
     CDS             complement(<1..658)
                     /locus_tag="ACFJAS_001440"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC3982670.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINTRLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     K"
     gene            complement(708..938)
                     /locus_tag="ACFJAS_001441"
                     /pseudo
     CDS             complement(708..938)
                     /locus_tag="ACFJAS_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(988..3993)
                     /locus_tag="ACFJAS_001442"
     CDS             complement(988..3993)
                     /locus_tag="ACFJAS_001442"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC3982671.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNANWDRQTNIFPEI
                     FVDSLCRINPDLPPDDARRLLADIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEAFRPDIALLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     PKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSACGKPVFNYFREEHKLNLTEL
                     LDTLSDDLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLAFLLQHGL
                     TYVKASQGSVQKHIMRYPQLFATLAIEKHLSNGGKKGVIWHTQGSGKTAPAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFVADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKEERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKANRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTAKELYNILRLQGSHEFFAHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDLEYISLR
                     EELERIFKKKNLAEVGQEEMQTNIATLETVYAKIKELNRQNDLLRHKYGGDAKYARIH
                     KRLMENAALYGDKLKVFNALSGVKTDADQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            complement(4113..5423)
                     /locus_tag="ACFJAS_001443"
     CDS             complement(4113..5423)
                     /locus_tag="ACFJAS_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC3982672.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            5620..5921
                     /locus_tag="ACFJAS_001444"
                     /pseudo
     CDS             5620..5921
                     /locus_tag="ACFJAS_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714068.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6601..9345
                     /locus_tag="ACFJAS_001445"
     CDS             6601..9345
                     /locus_tag="ACFJAS_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3982673.1"
                     /translation="MPGKKNTPIIRQNHTGWGVQSTIDSDVVLSAANNIVEVGGSPMN
                     QEGITAHGNATITLKAKENNKITVENAAYSSDGISTLINRTGARPGTRDDGNKIILEA
                     GGDNIVTMKSGDADADYVNNSKVLTETPYYKSKRGSNGIFAYGDKSLVKLIGENNIVK
                     SEISEKSKALNGGFRHIGIYSWQNAKVELSAKSDNIVQGGIWGLYSNNSSISLKGKNN
                     VISNPKYNVFAYKKAKVDLTVENKNTLSDAEFGVYALNTSMVNLSSKDNNEVKSTQVG
                     LYSQDGGSINVDRKDNIIEGDAVALVGKGGSQNIRASRTNLISSKSLGIHAEQAAKIA
                     ITGASNTIHASNAAIRSLDKSEVKIDGQITIDSNVANLARQDGSIHLNYKDDTRITGA
                     TVSDKGLVAIKPLNNTNIVADTIHYKGDVLAVNKGKVELDFTPNILLAGRLDNFSGLT
                     DSKHKNLFENYVANLDSKSAGEINFNLAKDALWTMTGQSWLDKLEGQGTIDFNNDAKT
                     SGRALHIGELAGANKFLMHLNKDGIHSDMLYVKKGTSTPQEVVVKNLSEVLDSMNYGE
                     RLRFATVTNSKNEFVNGKKYIDDTHLMEDALTVEYSAHNGDKNNKDDYNKSFNGSEMT
                     AEKAGDDYVNKTYTDNRQNVYLVKQATGNPSRNVKNINDMFDSTAHYAFTLDTYAKRE
                     GERAFSTLDKKEGDWIRLTHTRVIQSNAFRFHNNDFEIGYDRFSLNEQEKKRKWGISL
                     DYGHGRTSLWNTFGKDKIRKYELALYNTTQYIDKEGDETGYIDNVLKIGKLRNRVIAR
                     NHMGQLWGKGKYSNTLFSISTEYGRRKFLDDDKLWRITPQVQLQYSYLRGTGYRIDNG
                     INVNLSHANSLIGRLGLDVVRKFDGGKKLFYIKGNIFHEFLGSRSFKAFEGKSHYAQK
                     "
     gene            9658..9852
                     /locus_tag="ACFJAS_001446"
                     /pseudo
     CDS             9658..9852
                     /locus_tag="ACFJAS_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063514830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(9873..10789)
                     /locus_tag="ACFJAS_001447"
                     /pseudo
     CDS             complement(9873..10789)
                     /locus_tag="ACFJAS_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11043..12505
                     /locus_tag="ACFJAS_001448"
                     /pseudo
     CDS             11043..12505
                     /locus_tag="ACFJAS_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            12756..13124
                     /locus_tag="ACFJAS_001449"
     CDS             12756..13124
                     /locus_tag="ACFJAS_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982674.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGSIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            13197..14606
                     /gene="thrC"
                     /locus_tag="ACFJAS_001450"
     CDS             13197..14606
                     /gene="thrC"
                     /locus_tag="ACFJAS_001450"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC3982675.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            14653..15447
                     /locus_tag="ACFJAS_001451"
     CDS             14653..15447
                     /locus_tag="ACFJAS_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982676.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLALRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            15635..16411
                     /locus_tag="ACFJAS_001452"
     CDS             15635..16411
                     /locus_tag="ACFJAS_001452"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC3982677.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
BASE COUNT         4221 a         3875 c         4318 g         4233 t
ORIGIN      
        1 ccttcggaat ttcccgtttc aaggtttcgt caaacaccat gtcgccgcct gaggatttgt
       61 agggcttgcc gttggcgtcg gggaaatcga actgcacaaa ccagtagtcg tacagggttt
      121 tcgccatctc ttccaggcgc gtgttgattt gtttgttcag ggcgattttt ttgtccaaag
      181 cggataggac ggcggcgatg gattgttgga tttgaggttc tggaataggc aactccagtg
      241 tttttaatgc attttcattg acacgttgcc tgccggaagt tccttccata cattcaatcg
      301 ctcgttttct aaaatcggga ctgattgcaa aataatagag aaattcggga ttggtttcat
      361 tttttgctct taacacgata aattcggtag aaccgaaagc cacttctcca tcatctaaaa
      421 tatctacaaa agcggttttc ccattttcca aacacggggt aatcttagct aataaggtat
      481 caccattgcg aaacttagct ccaccgttaa atgccttgat ttcgtaaccc gtaatttgtc
      541 gttgaaattc tttcaacatt gccatcggaa cactttttgc caatgctccc ttggctagtt
      601 gctctctcgg attaaaatca gcaatatttt gaatttgttg tttcttaaaa tgattcatga
      661 tgttccgtta caaaactctt caaaggccgt ctgaaaaaaa catttcatcg gtagttcttg
      721 ccgtctgcgg cagttgtaaa ctgaaagttt acaactgaat cgtccagttc ttgttctgca
      781 aatatggctt tgatgtgttt accgatgttt tgtttgctgg tttggtagag ttcggcaatt
      841 tctttttggg taagccatag ttcgccgtca agctcgcgca gggtaaattc ggatagtccg
      901 tcttccgtgg tgtagatgat gatgtcgttg ttgtgcatag ctttcctgta tttataaatg
      961 gttaaggcta cctaaaaaac ctctttctca aatccgcccg ctttctttta aatattcccg
     1021 caccaacagg cggttgatgg cttggatgtc tgcaggctga acaggaaatt gctgttccgt
     1081 tctaaagcgt tttaatacga tgccttgcat ttgtttttca aagtaatttt ggttgtccaa
     1141 aatctgctcc atatccagca ctttttggtc ggcatcggtt tttacaccgc ttagcgcgtt
     1201 aaagactttg agtttgtctc cgtagagggc ggcgttttcc atcaaccgct tgtggatacg
     1261 tgcgtatttg gcgtcgccgc cgtatttgtg ccgtaagagg tcgttttggc ggttcagctc
     1321 tttgattttg gcatagacgg tttcgagtgt ggcgatgttt gtctgcattt cttcttgccc
     1381 gacttcggcg aggttctttt tcttgaagat gcgttccagc tcttcgcgca ggctgatgta
     1441 ttcaaggtcg tcttggtcga agttgcccgc cagcccttcg cgcactttgc gcatgatgtc
     1501 ttttaaatcg tcggcaagtt tgagttccgc ctcgccgatt ttgacgaatt gaaagtaaac
     1561 gtcttccagc gcttcattga gcaaatgggc ggtgtcgcct tgctgtaatt tttcggcaag
     1621 gtttaaggta tccaagcggg cggcggtttc gcggtacaaa agattgagct tgtcaaaatc
     1681 cagatgcgca aaaaattcgt ggctgccttg caggcgcagg atgttgtaca actctttggc
     1741 ggtttgcagg gcttttttta aggctagaag ctgttttttg tcttctattt ggctaatttg
     1801 gctgcaaaat tcttcggcgt tttcggtatc gaaatcaaac agggcgtttt taatgtctgc
     1861 aatttcctgt tcgatttctt cggcggtttt gaatagctgg ctgtaactgc cgatttcgtc
     1921 gcccaattcg ttggaaagtt cgtcccaata ggcgcggttg gctttgtcaa attcgcgttc
     1981 gatatcggca aaatcgacga catagccgta gcggtaagat ttgtaggtgc ggttgacgcg
     2041 ggtcagggtt tgtaaaaggt tatgggcttt tatcagtctg cccaaataga gctttttcag
     2101 gcgcggcgcg tcaaagccgg tcaaaagcat gttgtacaca aacaagatgt cggttttgcc
     2161 cgctttgaaa tctttaaccc attggtcgcg ttcttcctta gtgccgacgt cgtgcaatat
     2221 caacgcggcg gtgaaattgt ggtctgaggc cgtctgaaaa tgctcaaaaa gctggcgtgc
     2281 ctgttctgcg ctgtcgcaaa cgaccatggc accgaggctc tcgtcttggt tggtttttct
     2341 gaatttggcg aagtcgtcca agatgtagtc aagcatcggg tggacgaagt gcggatgggc
     2401 gtagatttct ttgcggtcaa agctgccttt ttcgatttca agttgcgcca gtgcttcttg
     2461 taattgcgcc ttgtatcggc tgccgatttc ttcgcgtatc aggcgcaggg tgtagccgtc
     2521 ggcaatggag gcgttgtaat agtatttgtg gatgtagtcg ccgaagagtt cgcgggtgtt
     2581 gacgttgcct gcggttacgc cgataagcgg cgtgccggtc agcccgattt tgacggcgtt
     2641 tacgtcggac tgattaaggt tggcaagaaa tgagcctttg gggttgtagc tgcggtgtac
     2701 ttcgtcgaga aaatagacgc gctgaatggc gaggtcgtag tcgttacggg cgacgacatc
     2761 ggggtcgtct tggaattttt ggatattaac aacagtgatt tccgctttgc ctgcgtggtt
     2821 gtgcaaagtt tgggcggatt tgatgtcggc aacgaaggct tcgcggctgt cgatggtatg
     2881 gacgactaaa tcgcgggcgg taaattcgcg ctgcgcctgt ttcaataaat cgagcctgtc
     2941 cacgatgaaa tagaatttcg gcacgatacc ctgtttggcg taatagtggg tcaggtaacg
     3001 ggtgttgtaa taggcaggcg cggttttccc cgatccttgg gtatgccaaa tcacgccttt
     3061 tttaccgccg ttcgataaat gtttttcaat ggcaagggtg gcaaacagtt gcggataacg
     3121 catgatgtgt ttttgcaccg aaccttgact tgctttgacg taagtcagcc cgtgttgcag
     3181 gagaaaggca agacgttcgc ggcaaagcag cgaagtcagg atgcggttgg tcggcgtatc
     3241 gggcgattta ttgctgataa attcggggct gtgtttgata acgggcagat tattgtcttg
     3301 tagaacgttg ttttcaaggt cgtctgaaag cgtgtccaat aattcggtaa ggtttaattt
     3361 atgctcttcg cggaagtagt tgaatacggg cttgccgcaa gcagacgagg cgtaaaacgc
     3421 gccttgtgcc ggctcggttg cgccgtcgtc gtattccatg ttgttggaaa aaatcatgaa
     3481 ttgggtgatg ttgataaaac ggcggaattt gggatttttc gcacgtttgc ccatgcgctc
     3541 ccgctcttcg ccgatgccgc ctttattgtt gggctttttg acttcgataa agaccaaagg
     3601 catgccgttt accagcaggg caatgtcggg gcggaatgct tcgtcgccgt tgatacaagg
     3661 caactcagtt acaacgtgga aactgttgtt gtcaaaattt tggaaatcga tcagcttctt
     3721 accgccggat tgattggtga ggcgttcgta aaatttttgc cccaaatctt cgttatccag
     3781 ctctaagcgt atgtcggcaa gcaggcggcg tgcatcatcc ggcggcaaat cgggattgat
     3841 gcggcagagg ctgtctacaa agatttcggg aaagatattg gtttgtctgt cccaatttgc
     3901 attttttagc gaaagataat catagcccaa gcgcatcagg tgcaaaacgg cggggatttt
     3961 gacgcgggaa ttttcgttgt gggtttggga cataaacgta acgtaatggg tcatcaaaat
     4021 ttattgaaac ggattataac acctgaaaaa agccgatacg ccttttcggt gtaccggctt
     4081 tgccatactg ttctgcttca gacgacatcg cttcattttg cctttaatgc ctcttcgtcc
     4141 agcgatttca accatgccag cttttcgccg attttgattt ccagcccgcg cgggacgggt
     4201 tggtaaaagt ccggttcgtc caagccgtcg ggcatataac tttcgccggc ggcgtaggcg
     4261 ttcggttcgt cgtgggcgta gcggtattcg cgtccgtagc ccaattcctt catcagcttg
     4321 gtcggggcgt tgcgcaggtg gacgggcact tcgtcgctgg cgttttcttt gacgaagcgg
     4381 cgcatttggt tgtatgcctt gtagcccgcg ttggatttgg cggcggcggc aaggtacagc
     4441 acggcttgcg ccagcgcgag ttcgccttcg ggcgagccta agcgttcaaa ggtggcggcg
     4501 gcatcgttgg cgatttggaa ggcgcgcggg tcggcaagcc cgatgtcttc ccaagcgata
     4561 cgcacgatgc ggcgggcgag atagcgcggg tcggtgccgc cgtcgagcat acggcaaaac
     4621 caatacagcg cggcgttcgg atgcgaaccg cgcacggatt tgtgcagggc ggagatttgg
     4681 ttgtagaaac tctcgccgcc tttgtcgaaa cggcggattt gcgcgcccaa gctgtcggcg
     4741 agaaattcgg tggtcaggat tttcagacgg cgcgtgtcgg cggcacgtaa aagttgttcc
     4801 aacaaattca ataatctgcg cgcatcaccg tcggcggtat tgacgagcag ctcttgcaca
     4861 tcggcttcaa tcgtgaaatc ccggtattcg ggcaaagcca acactttggc aatcagtttc
     4921 ttgaggtcgt ctgaagacaa ggattgcaaa acatatacct gcgcgcggct cagcagcgcg
     4981 ggattgactt caaacgacgg attttccgtc gttgcgccga taaaggtcag caaaccgctt
     5041 tcgacatgcg gcaaaaacgc gtcctgctgc gccttgttga agcggtggac ttcatcgaca
     5101 aacaaaatcg tcgcgcgtcc ctgctgcaaa gcgatttcag ccttatcgat tgccccgcgg
     5161 atgtccttca cgccggaaaa cacggcggaa acaggcaaaa actgggcatt gaaactctgc
     5221 gccaaaatcc gcgccaacgt tgtcttgccc acgcccggcg gcccccacag caacatagaa
     5281 tgcggcttcc cgccttccac cgccacgcgc agcggcttgc cttcgccgat taaatgctgc
     5341 tgcccgataa catcatcaag cgtatgcggg cgcagacgtt cggcaagcgg tgcgtcgggt
     5401 tctcggacaa acaaatcggt cataacggct ccgtcaacag gttttcagac aatatgatta
     5461 tacggcaggg aaaggcggcg tgccgcatac ggataccgcc ccaccgtttg tactaagccg
     5521 atcttaggcg tacaattggg aaatttcagg aaatttatac gctatgccat ccatgcagct
     5581 ttgcaaccgg acccgcaaag accaggtccc gttttaacca tgcggggaag ttaagattac
     5641 agtaaacaaa actattttgc cttattggaa gaaaggggaa cagttatgag aaattggaac
     5701 ttattaccac cggggaaggg gcagatggag acttactggt caacggttct tacggcatcc
     5761 gttcttttgc agacctaaaa ttggaataca aacccgaatt atcggtgaga agcaatcatg
     5821 acattatttt caacaatgat tttcaatctg ttgacgtaca accctattcc ggaaatatcc
     5881 atttggatgc caaaggaaaa attcaattta atttgaatgt tgccgataag gtaacagatc
     5941 caaccacaca tcaagaaaag ttagtgaacg ataacgcttt agtgcggtat gtcaacaata
     6001 tcaccccctc cgatgaaaat gacaaaggca gcatatcctt ccattcaaaa acaggcaatt
     6061 tgttcaatat gaacgttaac gttcataaag gcacacacca tttcggtatt cacaacctcc
     6121 gcaataccaa tgttaatctg actgccgaag acggcgataa tgtggttgcg attaaaggga
     6181 agcttgccga gcctatgccg gcaggaaaag ttgagagtac aagtttttta aggggtattg
     6241 aaacggcttt taaatccaat accgtattaa cggggaataa caataaaata tccatcacca
     6301 ctgagggcga cggcttggga ttgggcgtaa atggcctcca gtccaactcc ccctattccg
     6361 gacacacgac gggtactttc atcaataatc aaccaagcaa tactacccaa ccttcccttg
     6421 acggcaccag caccatcgaa ctcaaagccg tcaaggggaa taatgaagtt aatttaaatg
     6481 taaaaaacca cgcctccgta aaaggcatta tcacgtcgca ttctgcaaaa gcaacgcttg
     6541 aagccgagga ggataatatt gtccgggtta aaaatccgga caccaagacg gctttaatca
     6601 atgcctggaa agaaaaatac cccaataatt cgccagaacc atacagggtg gggggttcaa
     6661 tccacgattg acagcgatgt agttttgagt gcggcaaaca atatcgttga agtaggaggc
     6721 agcccgatga accaagaagg gattaccgct cacggaaatg ccacgattac cctcaaggcg
     6781 aaagaaaaca ataaaattac cgtggaaaac gccgcataca gcagcgacgg catttcgact
     6841 ctgattaaca gaacgggggc aagacccgga acaagggatg atggaaataa aatcatactg
     6901 gaagccggcg gcgataatat tgttaccatg aaatccggcg atgcggatgc ggattatgta
     6961 aacaattcca aagtattaac ggagacacca tattataaaa gcaaacgagg ttccaacggc
     7021 atttttgcct atggcgacaa atcgctggtc aaactgattg gcgagaataa tatcgttaag
     7081 agtgaaatca gtgaaaaatc taaggcatta aatgggggat ttcgccatat cggcatttat
     7141 tcatggcaaa acgcgaaagt cgaattgtct gcgaagagcg acaatatcgt acaaggcgga
     7201 atttggggct tatactccaa caactcctca atttccctca aggggaaaaa taatgtgatt
     7261 tcaaacccga aatataatgt tttcgcctac aaaaaggcaa aggtggattt gactgtcgaa
     7321 aataaaaaca cattatctga tgcggaattt ggcgtatatg ccttaaacac aagtatggtt
     7381 aatttgtctt caaaagataa taacgaggta aaaagcaccc aagtgggtct gtattcgcaa
     7441 gacggcggtt caatcaatgt agataggaag gataatatta ttgaaggcga cgcggttgcc
     7501 ttggtgggaa aaggtggaag tcaaaacatt cgggcaagcc gtacaaacct gattagttca
     7561 aaaagcttag gaattcatgc tgaacaagct gcaaaaatag ccataaccgg cgcaagcaat
     7621 acaattcatg caagcaatgc cgctattcgt tcattagaca aaagcgaagt taagattgac
     7681 ggtcaaatta ccattgactc caacgttgcc aatcttgcaa ggcaagatgg ttcaattcat
     7741 ttgaattata aagacgatac ccgtatcaca ggggcaaccg tatctgataa gggtttggta
     7801 gccatcaaac ctttgaataa cacgaatatt gttgccgaca ctattcacta taaaggcgat
     7861 gtcttggcgg taaataaggg taaagtggaa ttagatttca cgccgaacat ccttttagcg
     7921 ggacgtttgg ataattttag cggcttaacc gattccaaac ataaaaattt attcgaaaac
     7981 tatgttgcaa atttagacag caaaagtgcg ggcgaaatta actttaattt agccaaagac
     8041 gcattatgga cgatgacagg tcaaagctgg ctggataaat tggaaggaca aggcactatc
     8101 gattttaata atgatgctaa aacaagtgga cgcgccttac atatcggcga attggcgggt
     8161 gccaataaat tcttgatgca tctgaataaa gacggcattc acagcgatat gctctatgtg
     8221 aaaaaaggca cttcgacacc gcaagaagtc gtcgtcaaaa atctgtccga agtgctcgac
     8281 agtatgaatt acggcgaacg tttgcgtttc gctacagtaa caaactcaaa aaatgaattt
     8341 gtgaacggta aaaaatatat tgacgatacg caccttatgg aggatgccct gactgtcgaa
     8401 tactccgcac ataacggcga taaaaacaac aaggatgact ataataaatc ctttaacggc
     8461 tctgaaatga cggcggaaaa agctggagac gattatgtca ataaaaccta taccgacaac
     8521 aggcaaaatg tctatttggt caaacaggct accggcaatc cgagcagaaa tgtcaaaaat
     8581 atcaatgata tgttcgattc aaccgcacat tatgcgttca ctttggatac ttatgccaaa
     8641 cgcgaagggg agcgggcttt ttcaacgttg gataaaaaag aaggcgattg gataaggctg
     8701 acgcataccc gtgtgattca atccaatgcg tttaggtttc ataacaacga ttttgaaatc
     8761 ggatatgacc gattcagcct caacgagcag gagaaaaaac gcaaatgggg cataagtctc
     8821 gactacggcc acggcaggac atcattatgg aatacgtttg gcaaggacaa aatcaggaaa
     8881 tatgaattgg ctctgtacaa tactacccaa tacatagata aagaaggaga cgaaacaggg
     8941 tatatcgaca atgtattaaa aataggaaaa ctgcgtaacc gtgtgattgc acgaaatcat
     9001 atggggcaat tatggggcaa gggaaaatat agcaacaccc tattctctat cagcaccgaa
     9061 tacggccgcc gtaaattttt ggatgacgat aaattgtggc ggattacacc gcaagtacag
     9121 ttgcaatatt cctatttgag aggtaccggc tatcggatcg ataacggcat aaacgtcaat
     9181 ttaagccacg caaacagcct gataggccgc ttgggtttgg atgtcgtgag aaaatttgac
     9241 ggaggcaaaa aacttttcta tatcaaaggc aatatctttc atgaattttt gggcagtcgt
     9301 tcctttaagg catttgaggg caaaagtcat tatgctcaaa aatgaaatac gagggggact
     9361 tggtattccg ccggattggg ctacaatgcg cggacaggca aaaaaaacga atgtctttgc
     9421 cgatgcggaa aaaagagttt tccggcggga aaaaaggatc gtacaacatc cgcttatccg
     9481 tttcccatca gtttggctga atgatatcgg ggccgcgcca ttattcggca ggccccattt
     9541 tattgccaca cattgcctgc ccgattcccg tttcaacagc aaacccttcc aatattttgt
     9601 tcaaacttgg atcgcggcag atgggggcgt gcaagccccg caaggttttt ttggctttta
     9661 cggaacagca cgcatttcgc caaacggcaa aattgcatta acggaattga aaacttttgg
     9721 aattgtgcaa aacggcattt gcgtcagttt aacggcattt ccaaagggca ttttaagccg
     9781 tattcgagag aatgcggatg gcgttttcag tgttttcagc gtgataaaat tcaaatttct
     9841 attctaaaac aatcagcaaa gcaggattta tttcagttgt ccgagacaac gggcggctgc
     9901 accgcctgca ctccccatac tttttcaacg cactcgagca ggagtgagac gtggggatta
     9961 tcctcgtatt cttccgcata aatgaaattg agcggcatac tgtggcggca cgattcgata
    10021 atagccacgc ccgcgccttc ttttgccgcc gcttcggctt tgtttccctg caccattgcc
    10081 acacctatac cgcctgcaac caaatcgata agggtttggg ggtagtcgca caatatctgt
    10141 ttttttgggt gagagccggt tgctgcgcca aaactggtgc aggtgcttcc tacttccgga
    10201 cacgcccgac atttctatcc atacgcattc ttgcaggctc tgcggaaggg agcgggtcag
    10261 ccgggaatat tggctttgcg ggcaaatcag cgcgtaggtc aggttttgca ggaatacgct
    10321 gcggatgccg ccttgggcgg cgttgccgag tataaagccg ccgtgcaggg ttttgtgttg
    10381 gatgcgcgag aggatttcgc cgctcattcc gtattggatg tgcaggcgcg ttttgggggg
    10441 ctgtttgacc gatattgtcc gtcagcgcga cgagttttgc cgaatcgatg gggtggataa
    10501 tgcccaaact ggtctcttcc gaataatcgc ctgccagcgt tttggcaaaa tactccaact
    10561 tgtgtttgta ttgcagcagg gattccgctt cgggcaacag tatttcgccc gcccgcgtca
    10621 ataccatgcc tttccccgtg ctcctgaaca gcggcgtgcc gacatattct tcaagggctt
    10681 taatttgggc ggaaacggcg ggctgggaaa ggaaaagtcg tttggcggtt tgggtaaggt
    10741 tgccctcgtg cgcgacggcg acaaatgatt ttaattgtac ggcatccatg tatccctcct
    10801 tgtgcggatg ttttctatat ttgtgcaatc gaaatctttt aggcggattg ttgctgaaaa
    10861 ttaacttttt aatcaagtgg tttgtaaatt gtatcagttt tccggatgat gattattaaa
    10921 aaaaattggt tttattgcct gtttgggctt taaatggggt tacggcttcc gaacgcagcc
    10981 cgtatcaaaa agaaaagtca tgcgccccct tttacgaggc gcgatatata aggaggaagg
    11041 ttatggaaaa acataatggg acttatcggg atttacaccg tccggcttcg gaatttgcga
    11101 cgcgggacga atatttggaa catgaattgc agattatgca accaaaacgc tggcggccca
    11161 acctgccctt tcgcgattac cgcttcgagt gggaggattt gattcctgcg atggcgggaa
    11221 cgattggaaa agtggtgatg gtggggggcg gcggcgtttg ccgcaccttt ggggctgcct
    11281 gacagctttg tactggaaaa tgtgcgctat gagcttttaa tcgccgccgc gtttatctta
    11341 ttggtatcgg gctttttctg cccggcgcca acctgcccgg tacgcacggg ccgctgattc
    11401 cgatgattcc catcgttgtg tcggcaggcg ggcatccttt ggcgttcggc atttcgattg
    11461 cggttttagg tctgctgatg gctttatttc gcggcggcag tattatggcg aagctgacaa
    11521 gcaacggcgt atgcggcgga ttattactct atttgggctt tatcggcacg acggggcagg
    11581 taaaaaaatt gttttcgtgg gcaggcggtt ttaatatgcc ctacatcgct tttaccgtca
    11641 ttattgtaac gattgtgatg tacgctttgt tggagcattg gaaaaaacgc tggttggccg
    11701 tgcctttggg atgcttgatt gccggtgtgg cggcatttgc attgggtgcg ccgtttgagt
    11761 ttcacaccgc ccccggcctg cctccaatga gtcctgctta ttggtagggt gaaaacagcg
    11821 gctggcatct ggggttgccg acggcagaaa gttttttggt tgtctttcca tttgcggtat
    11881 tggctgttgc aatgtggtcg cccgattttt taggacatca agtgttccaa aaaatcagct
    11941 atccggaaaa aaccgataag gtattgatga atatagacga caccatgaca agttgttctg
    12001 tccgtcaagc agtgggttct attttagggg gtgcaaattt tacctcttct tggggaactt
    12061 atatcgtgcc ggcatcgatt gccaaacgcc ccattccggg cggtgcggtt ttaacggcgg
    12121 ttttatgtat tatcgccggg ttgtggggct atccgatgga cttggcgatt tggcagccgg
    12181 tattgagcgt agccttgatc gtaggcgtat acttaccgct tttggaagcg ggcatggaaa
    12241 tgacgcgcaa aggcaaaacc acccaatccg ccgccatcgt ggtgttctct tccgtctggt
    12301 caatccggtt ttcggctggg cgttgacgat gctgttggat aatttgggct taatcggctg
    12361 caaagaacgc agcgcgcaat taggttttgt cggacgagta ttgatacccg cagtaggttt
    12421 cttaatcttg tgtgtggcga tgggtgcggt cgggatgctg cccggtatcc ctccgttttt
    12481 ggagcagttc aaatctttgg gctaggctga aatcgcaaat gccgtctgaa ccggtttcag
    12541 acggcatttt tgcaaacagg caaaatgacg gcggcgggat tttttatttt cccgattgaa
    12601 gtataatgtt gccgggcttc aaccggatat tcaaacggtt tgctccaaca ctcggaacgg
    12661 cgcataaaac gccgcccttc gcgttatccc gaacggggcg gctaatcaga tcctatcgcc
    12721 ataaaaggcg gggtttcaac cgaaaaggaa ttgagatgaa taaaaccttg tctattttgc
    12781 cggcggcaat cttactcggc gggtgcgccg ccggcggcaa cacattcggc agcttagacg
    12841 gcggcacggg tatgggtggc agcatcgtca aaatgacggt agaaagccaa tgccgtgcgg
    12901 aattggacag gcgcagcgaa tggcgtttga ccgcgctggc gatgagtgcc gaaaaacagg
    12961 cggaatggga aaacaagatt tgcggctgcg ctaccgaaga agcacctaac cagctgaccg
    13021 gcaacgatgt gatgcagatg ctgaaccagt ccacgcgcaa tcaggcactt gccgccctga
    13081 ccgtcaaaac ggtttccgcc tgcttcaaac gcctgtaccg ctaaccttct tcagacggca
    13141 tgaggcagat gccgtctgaa tccgttttcc tgcaatttcg acaaacgaga aaaatcatga
    13201 gatacatcag cacgcgcggc gaaaccgcac acaaaccgtt cagcgaagtt ttattgatgg
    13261 ggcttgcacc cgacggcggc ctgatgctgc cggaacatta tccgcaaatc gggcgcgaaa
    13321 ccttggacaa atggcgcggt ttggcttatc ccgaattggc gtttgaaatc atgtgcctgt
    13381 tcgttaccga tattccggag gacgatttgc gcgacattct gaaccgtact tacacggaag
    13441 cggcgttcgg tactaaggaa atcacccccg tccgcacgct ttcagacggc atcaaaatcc
    13501 aagccttgtc caacggcccg acgctggcgt tcaaagatat ggcgatgcag tttttgggca
    13561 atgcgtttga atatgtttta aacaaagaag gcaaaaaact caatatcttg ggcgcaacca
    13621 gcggcgatac gggttcggct gcggaatatg ccttgcgcgg caaaaaaggc gtgaacgtat
    13681 ttatgttgtc gcccgacggt aaaatgagcg cgttccaacg cgcgcagatg tacagcctgc
    13741 aagacgggaa tatccacaat atcgccgtga aggggatgtt tgacgactgc caggacattg
    13801 tgaaggcagt gcagaacgat gccgcgttca aggaaaaata ccatatcggt acggtcaatt
    13861 cgatcaactg ggggcgcatc gtcgcgcaag tggtttatta ctttgcaggc tatttcaatg
    13921 ccacttcaag caatgacgaa accgtcagct tttgcgtacc gagcggcaac ttcggcaacg
    13981 tttgcgcggg acacatcgcc aaacaaatgg gcctgcctgt ccgctgcctg attgtcgcga
    14041 ccaatgaaaa cgatgtgctg gacgagtttt tcaaaaccgg cgcataccgc ccgcgcaaca
    14101 gcgcgcatac ttatgtaacc tccagcccgt ctatggacat ttccaaagcg tccaacttcg
    14161 agcgtttcgt gttcgacctg atggatcgcg atcctcagga aatcaatacg ctgtgggcgg
    14221 aagtcgcggc aggcaaaggc tttgacctgc ggtttgcctt ggacaaagtc ggcggcaaat
    14281 acggctttac ctccggcaaa tctacccacg ccgaccgcct cgccaccatc agacaggttt
    14341 acgagcaaga caaagaactc atcgacccgc atactgccaa cggcgtaaaa gtcgcccgcg
    14401 aagtgcgcga agcgggggaa acggtggttt gtttggaaac cgcgttggcg gcgaaattcg
    14461 atgcgaccat acgcgaagcc gtcggcgatg tcgccattcc gcgccccgcc gcgctggaag
    14521 gtttggaaaa cctgccgcag cgcgtgcaaa ccgtgccgaa cagtgcggat gcggtaaaag
    14581 gcatcatcga acaaaccctt gcctgatgtt ccgtatgggc ggcattccat ccgaacttaa
    14641 gattgaaaga aaatgacctt acctatgcag gaaacccgtt tttctatact gctggacgaa
    14701 ttggcggcaa aacaggaggc aaccatcgcc ccctacctgc ttgccgacgg gaccaaggta
    14761 tgggtacgca aagcaggcag gcacaatgcg cgatggcgtt acgcgctgct cggtatggtt
    14821 gcccgatatt tgaaactggg ggtgttgaag ccggttccca gcctcggcgg cgagcctgcc
    14881 attgcaaccg aatcaaaacg cctgtacgaa ttgcgttcgg cagggatagc cgttcccgaa
    14941 ttgctcgccc tccggaaaaa tgcccttatg ttcggcaatt tggaaggcat cccgctcgat
    15001 acgcaaatcc gccaagaagc cgaagccgga aaggcggacg cttggcttgc cggtttggaa
    15061 gccattgcac gcgtgcataa aaaacggcag ttcctcagtc aggcgtttgc acgaaatatg
    15121 atgtgggacg gaaaaaacat cagctttttg gattttgaag acgacccttc cgaggtcctg
    15181 accatagcgc aatgccaagc ccgcgattgg ctctgctaca tccattcgac cgcgctgata
    15241 ttgaaaaacg gcggactgct ggaagcggct gcggaaaaat ggggcggcgt attgtcggat
    15301 cagcctgccg aaatacaaaa gctgattgcc ggcaccgtca aaccgattct cccgatacgc
    15361 aggctggaac acccccgctg gggacgggac gcgctcaggc tggcagcctc gatttcctta
    15421 atttccctcg ccgatatgcc gccgtaagcc accgcccgcc ccacgatgcc gtccgaagcc
    15481 cttcagacgg catataacca acgcgcatca ggcattgtcg caaccatccg tttcgtttag
    15541 aattttgact ttatcccgaa tcgggcagac atttccactc tgccgcacgt cccgaacaag
    15601 cactatccat ttcttttact gacaggaaac ccaaatggca gcattcaata cccaaaaagt
    15661 attgtccgta caccactgga cagacgcata ttttaccttt acctgcatcc gcgacgaatc
    15721 gttgcgcttt gaaaacggac agttcgtcat ggtcgggctg atggcggacg gcaagccgct
    15781 gatgcgcgca tacagcgtcg cctccgccaa ctgggaagaa cacctcgaat ttttcagcat
    15841 taaagtccaa gacggcccgc tgaccagccg cctgcaacac ctcaaagtcg gcgacgaagt
    15901 gttaatcagc aaaaaaccga ccggaactct ggttgcctgc gatctgaatc cgggcaaaca
    15961 cctttacctg ttgagtaccg gtaccggcat cgcccctttc ttgagcatca ccaaagaccc
    16021 cgagatttac gagcaatttg aaaaaatcat cctcgtacac ggcgtgcgct acaaaaaaga
    16081 tttggcgtac tacgaccgct ttaccaaaga attgcccgaa cacgaatacc tcggcgactt
    16141 ggttaaagaa aaactgattt actacccgat tgtttcccga gaagaattcg aacaccgcgg
    16201 ccgcctgacc gacctgatgg taagcggcaa actgtttgaa gacatcggcc tgcccaaaat
    16261 caacccgcaa gacgaccgcg cgatgctgtg cggcagcccc gcgatgctga aagatacctg
    16321 caaagttttg gacgatttcg gtctgaccgt ctccccgaaa accggcgtgc gcggcgatta
    16381 cctgattgag cgcgcgtttg tggatcaata agctgcttga aaatgccgtc tgaacggcat
    16441 cagcgataaa aaaccccgaa tcctgccaat caggattcgg ggtttttata gtgggtaaaa
    16501 attagaaatg cacatttccg tcattccaac acaggtggga atccggactt acctgtttca
    16561 ttatcgtttg aaaataaaag agaaaccaaa catatagatt cccgcttgcg tgggaatgac
    16621 gacggtgttt tgccgtagcc aaagcag
//
LOCUS       DAXFZR010000054        15489 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000054 DAXFZR010000000
VERSION     DAXFZR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15489
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.054"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            69..281
                     /locus_tag="ACFJAS_001453"
     CDS             69..281
                     /locus_tag="ACFJAS_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC3982678.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            466..990
                     /locus_tag="ACFJAS_001454"
     CDS             466..990
                     /locus_tag="ACFJAS_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFC3982679.1"
                     /translation="MTDRQIDNLIKPATIADTDPASNRVRAQHGGITTDWLPYIVPFA
                     GGVSVWRIPSVGEACTILSPAGEPENGVVLCCQASDRYPAPSADPAETVVRFPDGTHI
                     RYNHNSGAMELKAVTSLTIDTPQTTITGHLTVNQTTTAQGLLTYQNGMNGQGGSLSEH
                     THPDDSGGTTEKPQ"
     gene            987..1313
                     /locus_tag="ACFJAS_001455"
     CDS             987..1313
                     /locus_tag="ACFJAS_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFC3982680.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1574..2044
                     /locus_tag="ACFJAS_001456"
                     /pseudo
     CDS             1574..2044
                     /locus_tag="ACFJAS_001456"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            2220..2783
                     /locus_tag="ACFJAS_001457"
     CDS             2220..2783
                     /locus_tag="ACFJAS_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC3982681.1"
                     /translation="MNSTVPANNSPLQHALAKLTERETAAVSRQLDPARCDPGFLPFH
                     AFARSIGTEEGWDFAETDEARRNLIAGFAEIHARKGTPYAIRALFPILRLGEIQIIER
                     DGEFKWDGSVLFDGSRTFGRREGDWAEYRIVLTRPVSIRQTARIRAMLAEIAPLRCEL
                     TALDYRNHPHRWNGKIRFNGEYGFGTT"
     gene            2823..5074
                     /locus_tag="ACFJAS_001458"
                     /pseudo
     CDS             2823..5074
                     /locus_tag="ACFJAS_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5186..5515
                     /locus_tag="ACFJAS_001459"
     CDS             5186..5515
                     /locus_tag="ACFJAS_001459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982682.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5531..5842)
                     /locus_tag="ACFJAS_001460"
     CDS             complement(5531..5842)
                     /locus_tag="ACFJAS_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFC3982683.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5850..6129)
                     /locus_tag="ACFJAS_001461"
                     /pseudo
     CDS             complement(5850..6129)
                     /locus_tag="ACFJAS_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6214..6827
                     /locus_tag="ACFJAS_001462"
                     /pseudo
     CDS             6214..6827
                     /locus_tag="ACFJAS_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6806..6970
                     /locus_tag="ACFJAS_001463"
                     /pseudo
     CDS             6806..6970
                     /locus_tag="ACFJAS_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
     gene            7072..7206
                     /locus_tag="ACFJAS_001464"
                     /pseudo
     CDS             7072..7206
                     /locus_tag="ACFJAS_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
     gene            complement(7290..7673)
                     /locus_tag="ACFJAS_001465"
     CDS             complement(7290..7673)
                     /locus_tag="ACFJAS_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC3982684.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGDTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            complement(7778..7918)
                     /locus_tag="ACFJAS_001466"
     CDS             complement(7778..7918)
                     /locus_tag="ACFJAS_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982685.1"
                     /translation="MPRYRNWQSGLDAKCADGDEYGVAQSDCRTREINTKTKEIQGYL
                     ID"
     gene            complement(7963..8415)
                     /locus_tag="ACFJAS_001467"
     CDS             complement(7963..8415)
                     /locus_tag="ACFJAS_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFC3982686.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            complement(9193..10839)
                     /gene="pgi"
                     /locus_tag="ACFJAS_001468"
     CDS             complement(9193..10839)
                     /gene="pgi"
                     /locus_tag="ACFJAS_001468"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC3982687.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            complement(11065..11913)
                     /gene="hexR"
                     /locus_tag="ACFJAS_001469"
     CDS             complement(11065..11913)
                     /gene="hexR"
                     /locus_tag="ACFJAS_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC3982688.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(11962..12948)
                     /locus_tag="ACFJAS_001470"
     CDS             complement(11962..12948)
                     /locus_tag="ACFJAS_001470"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC3982689.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            complement(12929..13624)
                     /gene="pgl"
                     /locus_tag="ACFJAS_001471"
     CDS             complement(12929..13624)
                     /gene="pgl"
                     /locus_tag="ACFJAS_001471"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC3982690.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            complement(13775..15220)
                     /gene="zwf"
                     /locus_tag="ACFJAS_001472"
     CDS             complement(13775..15220)
                     /gene="zwf"
                     /locus_tag="ACFJAS_001472"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC3982691.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
BASE COUNT         3670 a         4659 c         3977 g         3183 t
ORIGIN      
        1 ggtgtggaat ttagggataa tctagtacag ccacaaaaaa tatgcgaaac aaaataaacc
       61 gcaacgacat ggaactcggc tacacgcctt acaaccttcg caccctgcgc aaccgctgca
      121 aactgacaca ggccgagttg gcgcaaatcg tcggggtgaa gcactacata caggtaggcc
      181 gctgggaagc cgaacccgat accgaaacgc ggcgggccga tatgccgctg gaaaaatggc
      241 ggcagttcct cgattggata gaaaaaacaa acgccgtctg aaaccttcag acggcatttt
      301 ttacccaccc ccaaacctaa aaaccacaac caagccctac accgcttgcc cccacctcgc
      361 cgccgcccca aaataacctc actctgttgg aagcctgccg gaaacggcaa aagccgcccc
      421 gttccgccat tacggggcgg tttcccacat caagacgaga cagaaatgac cgaccgccaa
      481 atagacaacc tcatcaaacc cgccaccatc gccgacaccg accccgcgtc caaccgcgtc
      541 cgcgcacaac acggcggcat taccaccgac tggctgccct atatcgtccc ctttgcaggc
      601 ggcgtgtcag tatggcgcat cccaagtgtc ggcgaagcct gcaccatctt atcccctgcc
      661 ggcgaacccg agaacggcgt agttttatgc tgccaagcct ccgaccgata ccccgccccg
      721 tccgccgacc ccgccgaaac cgtcgtccga tttcccgacg gcacgcacat ccgctacaac
      781 cacaacagcg gcgcgatgga attaaaagcc gttacaagcc tgaccatcga caccccccaa
      841 accaccataa ccggacacct gaccgtcaac caaaccacca ccgcccaagg gctgctgacc
      901 taccaaaacg gcatgaacgg ccaaggcggc agcctgtccg agcacaccca ccccgacgac
      961 tccggcggta caacggaaaa accccaatga ccgacgcaga aaacggacgc gggcaagaca
     1021 ccctcgccca catcgcccaa tccatccgca acatcctgtt cacaagaata ggaacgcggc
     1081 tgatgcgcga agaatacggc agttttattc ccgacttgat cgatatgccc gccggtcacg
     1141 ccgccatcgc cctcatccac caagccgccg ttaccgccct cgcaagatgg aaaccccgca
     1201 taaccgtccg ccgcatacag gcagacaccg ccgacctcgc cgccggcaaa atcaaattaa
     1261 ccctagacgt aacccttgcc gacggcggcg aacgaaccta ccgcatcaaa taataaaaac
     1321 aacaggataa acaatgggaa acagccgatt aagccaactc cccgcgcccg ccgccatcga
     1381 agaaaccgac tttgagggca tcttcgcgcg caaaaaagcc gccctcaccg ccctatgtcc
     1441 cgaaagcatc cgcgaaaccg tcgcccaaac cctcgaatta gaatcagaac ccctaaccat
     1501 cgacctgcaa cagcaagcct atcaagagct gctcgtccgc aaccgcatca acgaagccgt
     1561 caaagccaac ctcttggcat acgcacaagg cagcgacctc gaccacatcg ccgcccaata
     1621 cggactttca cgcaaaacca tccgcgccgc cgaccccgac gcgaacccgc cggttgccgc
     1681 cgaatacgaa accgacgacg cattccgcgc ccgcgtccaa gcccatcccg aaaaatacgc
     1741 cgccgggccg cgcaccgcat acgaagccca cgccatcgac gcacaccccc aaatcacaca
     1801 cgcccgcgcc gtgcgccgcg ccgccggcac ggtggaggtt tacatcaaaa cccaaagcgg
     1861 cacgcccgac gaaaccattt tgaccgccgc gcgcgaatac ctgcccgccg aaacacgccg
     1921 ccccctttgc gacaacgtgc aagtaaccgc cgcccaaccc aaagacgcgg cagtagagta
     1981 ttccgccgaa taccaccccg ccgccaatat tcaagccgaa cggcagaccg cctgcgaagc
     2041 cttggataac ctatggcggc aaaacgccca catcggcgca tccgtcgccc tgtctaaaat
     2101 catcggcgcg ttagacaccc ccggcgtgaa aaaaaatcac actgcacagc cccgctgccg
     2161 acatcgaatg cggcaacggc gaatacatcc gaatcacgtc cacgctcgag cgcgaataaa
     2221 tgaacagcac cgtccccgcg aacaacagcc ccctgcaaca cgcactggca aagctgacag
     2281 aacgcgaaac tgccgccgtc tcccgccaac tcgaccccgc ccgatgcgac cccggatttt
     2341 taccctttca cgccttcgca agaagcatcg gcacggaaga gggctgggac tttgccgaaa
     2401 ccgacgaagc ccgccgcaac ctcatcgcag gctttgccga aatccacgcc cgaaaaggca
     2461 cgccgtacgc catccgcgcc ctcttcccca tcttgcggct gggcgaaatc caaattatcg
     2521 aacgcgacgg cgagttcaag tgggacggct cggtcttgtt cgacggcagc cgcacattcg
     2581 gcaggcgcga gggtgactgg gcggaatacc gcattgtctt aacgcgcccc gtcagcatcc
     2641 gccaaaccgc ccgcatccgc gccatgttgg cggaaatcgc ccccttgcgg tgcgaactta
     2701 ccgcgctcga ctaccgcaac catccccacc gctggaacgg caaaatccgc tttaacggcg
     2761 aatacggttt cggcacgaca taacgcgccc ccgaaaatca acaaacaaaa ggaagcccca
     2821 aaatggcaaa cgcaaccgaa caaaaccaat tcgaccaagc cgtccgcctc atcgaacccg
     2881 gcgacagcgt cgtcgtcggc ccaggcgcgc ccgtcaacca accactgcaa gccctcgcca
     2941 accgcaccct tttgctcaaa aaccaaaccg aagcccttca gacggcatcc gacaccaaag
     3001 ccgccgcaag caccgccgtc aacgcaggcg acggactgac gggcggcggc agccttgccc
     3061 aaagccgcac catcgcactg ggcgcgcccg ggcaaatcac cgcaacaagc caaaacaccg
     3121 tcccaaaaaa cggccacacc cacgccatcg acaccgcacg caccgaccgc gccggcatcg
     3181 tccgattaga caatgccatt agcgaagccg aagacaccgc cgccaccccc aaagccgtca
     3241 aaaccgcgct cgaccaagcc cgcgccgccg ccgcgaccgc cgacctcaaa gtctccctct
     3301 ccgacaacca aaccgttacc ggccaaaaaa cctttaccgc cgaaacacaa ttccaaagcg
     3361 gcatccggct gtccgccaac ccgacgcact gaaacggcgg ctacaaagcc tatatcggcg
     3421 cggataacga aaacgcccac atcgtcttcg gcgacgacac cctgcgcctg cacggcgcaa
     3481 ataaccgcat ttcctacaac aaccacgaca tcttccacaa agccaacaaa ccgcgtttca
     3541 acgaagacat cgaaggcaaa ccgaacacgc tgtccggcta cggcatcggc aacttcaaag
     3601 tcgaaacatt ccggggcgat ttgaacagcc tcaaaaccga cggcgtctat tccctgccga
     3661 cggcggtcgg cagctcgaac ctgcccgttg aaaacaccgc ctgccatatc caagtcatcg
     3721 ccggcacgca acccggctgg tgcaggcagt tgggctatcc cgcctacacg tccgacgtgt
     3781 acgaacgcta ccagaccagc agcgcaaacg acgactggag cgcgtggaaa aaactcaatt
     3841 cggaaggcat ccccgccggc gcgatcgtgt cctttcccaa agccgtccga aaccccgcag
     3901 gctatctcaa agccgacggc acgatctttg cacaaaacac cttctcgacc tttaccgcgc
     3961 cctgggcaac acaaacaaac tgcccgacct gacccgtacc gacatcggca tcaccgcgtg
     4021 gtttccgtcc gaccaaatcc agacgggctg gctggcgttt gacgacatcc gagagcgcgt
     4081 aaccgaatcg gcttatcccg aactttaccg cctgctgacc gaaaaatacg gacgcatcca
     4141 aaacgtcccg caggcggaag accgctttat ccgcaacgcg ggcaacggct tggcggtcgg
     4201 aacgaagcag gaagacgaaa tcaaacggca cgtccacaaa gtattttcac actgggcaaa
     4261 ccacccagac gccgccgccg tcggttacga agaccgcaat gaaaggcaaa gaagcgcgct
     4321 cgtatcgact tggacggacg gcgacttaaa cgacaacggc tttttaaccc cgcgattgga
     4381 cagcaaaatg gcaacgggcg gcgcagaaaa ccgccccaaa gccctggttt taaaactgtg
     4441 catcaaagcc gccgacacct tgggcgaagc cgtgttccgg ataaagtccc acggcgaaac
     4501 cgccaacgcc ggcgcactgg acgcgtcaag gctggcgcaa ggtttgcagg aaaaagccga
     4561 ccgcgaccac acccacacca ccgcccaaat ccaagggctg gacgaaaaaa tcagcgccgc
     4621 cgttgccgcg caattcacac gccaaaccat cggcggcgtg gatattgtca gattccccga
     4681 cggcacaatg atacagaccg gcagttacag gtttgcaaga ggcggcagcc ccataggaaa
     4741 cgaagttgtc ttccccatcg cctttgccga cggcaacgtc aaatgcttcg tatccgaacg
     4801 ccattcggga cgcgtcaacg gcgaaaggca acacaactgg ctgtttatcc gcgcaaaaaa
     4861 ccacgccgcc gccattatta ccaactggta cgaaagcagt tgcgactgga tggccatcgg
     4921 caaatccgcc tcgggaaacg ccgccagccc caccccgata gtccccgaaa tacctgaaat
     4981 cgatgaagaa ccgcaaagag agagtggaag atcatcaacc ggactccgaa acccccgccg
     5041 ccaccgaggc ctcgacttcc ccgtggggtc gtaagactag cgggcggcag ggcaggctgt
     5101 agagacgggc tgtagagacg ggcttcagcc caccgatcca agcaatccga ccgaaaccgg
     5161 ccgcgccgcc aatcctgcga aacctatgcc ccgccaatcc tgccactctt cgccattccc
     5221 gcgaaagcgg gaatccggaa cccaaacgcg gcaggaatct atcggaaaga ataacccgac
     5281 cgccgtcatt cccgcgcagg ctggaatcca gacccacaac gttaaggcgg tttatcagaa
     5341 aaaaccgaaa ccgaacgccc tagatttccg cctgcgcggg aatgacgaag agttggggag
     5401 tgacgaaagg cgggaacaac cgcgcaaaaa gccgccgact ccttcagacg gcatcggcaa
     5461 taaaaaccgc acggccgaaa ccgcgcggga aaggatagcc gggcgcgccc gataagcagc
     5521 ggccgcaggg ttaagtcctt gcacctgttt tcatcgaaga caaaggcggt tcgggaaata
     5581 agggcgcaat atcaaaagtt ttgcgttgcc tgatttgcca caaatcataa ccggcctgca
     5641 tattcagcca aaaatcaggc gaagtgttga gcagttggct taaacgaacc gccatctcgg
     5701 gcgtaatcga agattgggcg ttgacaatgc gcgataaggc aacccggctg acacccaggc
     5761 gtttcgctat atcggtaatg cttgcgccgt taatatactc ttttaaaaca aggcccgggt
     5821 gggcaggatt gtgcattttc atattattcc tagtgataat cctgataatc gacaacttcg
     5881 acattgccgt cgttgaaacg aaaagtcaaa cgccaatttc cgttgacttt gaaccgccca
     5941 atgtcccgac aaactgccct tgaggggatg aagattccaa ctcggcacag ccatatccga
     6001 aggcgtttgc gcggcattca gcgcggttag aagcagattc agtttgacgg aatgcccggc
     6061 ttggatgccc gatagcgagc ctgttttaaa aaagcgttca agccctttgt gtttgaaaga
     6121 aacaatcatc atcccacccc gtctgtatcg ccaaaggata caaaactttt atttgattgt
     6181 aaagcgacgg tatacaaaaa aggaaacccc gaaatgacca tctatttcaa aaacggcttt
     6241 tacgacgaca cattgggcag cgtccccgaa ggcgcggttg ccgtcggcgg cgaagaatac
     6301 gccgccctct tcgcaggaca ggcgcagggc gggcaaattg ccgccgattc cgacggccgc
     6361 cccgttttaa ccccgccgcg cccgtccgag taccacgaat gggacggcaa aaaatggaaa
     6421 atcagcaaag ccgccgcccg tttcgccaac tcccgtttcg ccaaacaaaa aaccgccttg
     6481 gcattcagcc tcgcggaaaa ggcggacgaa ctcaaaacag cctcttggcg ggctatcccc
     6541 aagtggaaat cgacagcttt tacaggcagg aaaaagaaac cctcgcgtgg caggcggaca
     6601 acgacgcccc gaccccgatg ctggcgcaaa tcgccgccgc aaggggcata gaattggacg
     6661 ttttgattgg aaaggttgtc gaaaaacccg cccgcctggc cgttgccgtc gacgcgatta
     6721 tcggaaagcg gcagcaactt gaagatagat taaataccat cgaaaccgcg ccagcattag
     6781 atgcgttaga aagggaaatc aaagaatggg cgctaaacgt cggctgagag aatatattta
     6841 tcatctacta gtggccatag accaactgtt caacgctata accggcggcg cggcagacga
     6901 aaccctctca agtcgcacct atcgcggcgc gcggctcgcc caacaaccca aaacccgctg
     6961 gaagatttta tagtggatta aatttaaatc aggacaaggc gacgaagccg cagacagtac
     7021 aaatagtacg gcaaggcgag gtaacgccgt accggttttt gttaatccac tatatatacc
     7081 ctgatcaacg gcgtattttt cgaccgccac cactgccggc aggcgtatat cagcgaactg
     7141 aaaggcaggc agcacgacgc gcggttcaac caaagccgcg ccgccaatga aagggatacg
     7201 cggtaaaatt gccgccgcgc cgcattcaga cggcatcggc aataaaaaag cccgaaaaaa
     7261 tcgggctgaa agttaaggct gcatcgcctt taaggcggct ttcgccaaaa aaccgctcgg
     7321 gtttcatgac gggcggcagt gcaggcatcg acacggttga ggatatgttg cggccaactg
     7381 acgttaaacc gcacctgtcc ggttaatgcg gcatcgtccg cttcaaccat cgtccacaac
     7441 accgcatcgg cataatcggg attgtcccga ttaacctcta tgctttgcgc ctcgggaatc
     7501 ggcaggtttt cctgcaaaag cccttcgaga tggaacattg cggcggcttt ggcatcggca
     7561 agcgcgtcgg caaccgtatc gccgacagga tagcagcccg gcagatcggg aataatcaca
     7621 ccccagttgc tttgatcggg gtcttggaac agcacggcgg gatagtaaat cattacttcc
     7681 tttctcggat atgcggtatc ggcaaagtcc aaacgcaacg aaggggattt aaaaacgaca
     7741 aaaacgcaaa agccgccgac attcccgcat ccaagtttca gtcaatcaga taaccttgga
     7801 tttctttggt tttcgtattg atttctctgg tacggcagtc agattgtgcc accccgtatt
     7861 cgtcgccgtc ggcgcatttg gcatccaaac cgctttgcca gttgcggtac cggggcataa
     7921 cggtttcggc aatatcggcg ggcagtgcct gaaccgcgct gttcagagcc gctttggcag
     7981 ttctttcttg tcctcttccc tgaccatttc gtcgataagg gcttttttac ggttgtgcag
     8041 ctcttccaag cgtgcttcgg tttctgccgt tttacacgcc aactcactga cttttatgcc
     8101 gtttggcgtt tcaccttcgg ctttcgcatt gttggcacag atcttgtcca taccgctttt
     8161 ccatgttttc tgcgaggctt gcagcttatt ctgtacagtt tgaggcaatc ctttccaaaa
     8221 ctgctcgaac tcgctgcgcg acagcttgta gcgttcttcc cattcggata cgcgggcggc
     8281 ttcctgctcc cgacccaatg cctcctgcgc cgccgcttct tctgcctccc tcgcttcctg
     8341 cgcggtggct tctgccgctt caagtttttc gttcctttgt ttcgctttgt ccaacggctc
     8401 tttaatcagt gccatagaca ccagctcgct gagcggatgg atgatatcgt gtgcctgact
     8461 gagttcggca aagattttgc ttttgtcgtc tgtcggctgg acgctgtaag aaatagtttt
     8521 gacataatac gcgccttctt tgcggtagta gggttcaaaa aagtcgggcg ttttcttatg
     8581 gctgttgcct atagattggt tggcggcgac ggcataatcg acaacatcgt cgggcacgtc
     8641 cagttttaac gcggcttcac atttgagttt gctggacgtg tcggttttct cggtagttcg
     8701 gacttcatcg acggttatgc ccaaacgttc gaccattctg cggatatgcg atatgccgat
     8761 atcggcaaat gcttggtctt tgaatgcgtc gtcgtcaaaa gttttgacgg tttcaccttc
     8821 gacattgtcg cggaccagtt tgaccaaaat ctctttagtc tcttcgcttc cgcaggaaat
     8881 ttcctttccg gaaaacgggt tgcctattcc caaacggtcg cagcccgaca aaatcagcat
     8941 acttgccaat gccagtgcgg gtaaattttt tttcattaga tttcctttgc cgtccaaaca
     9001 cggactgtct gttgcgtatt tccacacacg ctacggcact tgaagtacat ggctgcggaa
     9061 catatatgtt taaatttatg caaaccgcca tatcgggatt gcacacgccc tgcaaattta
     9121 ccgacggttt tcctgttcga taaaaatgcc gtctgaaacg gtcggcgttc agacggcatt
     9181 tttccgcagg ttttatttgc ggttggtctg caggtagagg ttaatcaggc gttcggtcga
     9241 gctgtcgtgt ttttgaggtt cggtttcgcc tgtcagttcg cccaaaatgg ttttagccag
     9301 ttgtttgccg agttccacgc cccactggtc gaagctgttg atgccccaaa tgatgccttg
     9361 gacgaaggtt ttgtgttcgt acatggcaat caggctgccc atattgcggg ggttgacctt
     9421 atccatcagg atgaggttgg tcgggcggtt gccggagaag gttttgtgcg gaaccagctc
     9481 ttcgatgcgc gcctcatcca taccctgcgc tttgagttcg gcgcggactt cgtcgggggt
     9541 tttgccgcac ataaaggctt ctgcttgtgc aaagacgttg gcaagcagga tttcgtggtg
     9601 tccgggcagg ttgctgcgtt tttcaagcga ggcaatcagg tcgatggggg tgatgtgcgt
     9661 gccttgatgc agcagttgga agaaggcgtg ctgaccgtta atgcccgttt ctccccagat
     9721 aatcggcgag gtttcgtgtc cgactgcttt gccgtccaac gtaacctgtt tgccgttgct
     9781 ttccatatcg agctgctgga tgaatttggg caggcggtgc aaatgttggt cgtaaggcgc
     9841 gatgacgtgg ctgccgccac cgtagtagtt gatataccag atgccgatga gggcgaggat
     9901 gacgggcagg ttgcgctcga gcggggtgtt gatgaagtgt tggtccatca ggtgcgcgcc
     9961 gttgagcatt tcgatgaagt tttcttcgcc gagatacagc ataatcggca atccgatggc
    10021 ggaccacagg ctgtaccgac cgccgaccca atcccaaaat tcaaacatat tggcgatgtc
    10081 gatgccgaat tcggcgacgg cttttcgatt ggtggaaacg gcggcgaagt gtttggcaac
    10141 ggcttcttcg tcacccgcat gattcaaaaa ccattcgcgc gcggttagcg cgttggtcag
    10201 cgtttcctgc gtggtaaatg ttttggaggc gatgatgaac agcgtggttt ccgggtggac
    10261 tttggacaat acgtcgcgca gttgcgagcc gtccacgttg gagacgaagt gcatattgag
    10321 gcgcggatga ccgaaaggtt tgagcgcggt acacatcgtc agcgggccca aatccgatcc
    10381 gccgatgccg atgttgacaa cgtcggtaat gacttggttg gtatagccca gccagcttcc
    10441 gctgcggact tcgtgtgcaa attcgcccat acgttgcaaa acgcggttga ctttgggcat
    10501 cacatcttca ccgtcaacca taatcggcga attggtgcgg ttgcgaaggg cgacatgcag
    10561 gacggcgcgg ttttcggtgg tattgatttt ttcgccgtgg aacatctgcc gcatccgctc
    10621 cggcacgcct gcttcacgcg ccaactcgaa cagtagggac atggtttcgt cgttgatgcg
    10681 gtttttggag tagtccagcg tcagtccgcc gacttgcagc cagtagcgtt ccgcccgctg
    10741 cgggtcttgc tcgaacattt cgcgcatatg caatgttttg ctgtcgtcaa agtggttcca
    10801 caattttgac caggccggta agtcgtgaag gtgtttcatc tgtatgctcc cgattgaggt
    10861 tttccgtgac ggggggcgtt tccgcacatc gatgcgcaca taaggcgcaa ccctgcgtac
    10921 cgccccgatt tggaaggggt tgcggcaaac ccgaaccctg ccgccttaaa gggcggggtt
    10981 tccgccatta aggaaattct gataaaaggc ttgccggagc ctgtccggcc gttcagacgg
    11041 cattggcggg atgccgtctg aacatcagtc tttgtcgtaa tcgatgtgct tgttgtgtat
    11101 gctttttttg cttttctgca attgcaggct ggcagcatcg cccaagcgca gggcaagtcc
    11161 gatggcgaga atatcgatga cggcaagctg caagaggcgg gaaaccatgg gcgtgtagag
    11221 ttcggcgttt tcctgtgtgg caacgctcaa cacgcagtcg gcaagttgcg ccagaggcga
    11281 atcgttgcgg gtcagtgcga tgacggacgc gccgttttct ttggcgatgc tgaccgcatc
    11341 caaaagttcg atagacgaac ccgtgttgga aatggcaacc aaaacatcct gatcgctcaa
    11401 aacggatgcc gccatcagtt gcgtgtgcgt atcgacatag gcgacggtgg acatgccgaa
    11461 acggaaaaat ttgtgctgcg cgtcctgtgc cacaatgccg gaattgccca cgccgtaaaa
    11521 ttcgacgcgg cgggcgtgca tcagggtggc gatgccgttt tcgagttcgg attcttttaa
    11581 gaacctcctc tcccccaaca aggctgcggc ggcgttgccc aacacttttt caaccacatt
    11641 cgccatatcg tcgtcggcat tgagttcttc gtggacataa ggcatacctt cgtgcccgat
    11701 gctggcggac agggagagct tgaattccgg cagcccctta taaccgaggc tgcggcagaa
    11761 ccggatgacg gtcggctggc tgaccgaggc gcggtcggcg atttcggcga cggcggcgtg
    11821 gacaaaccac ttgggttctg ccaaggcgca ttcggcaact ttgcgttccg caccggacag
    11881 gtcggccagt gattcgctga ttttgcttaa cataatgata tgcccttcga taatgcggcc
    11941 ctgctgcaag ggctgccgtg gttagacgtt tttcaggtgg ttgccgagcg ccgccgccgc
    12001 gccggcaata cccggaaatt cgctcaagac gacataaacg ggaatcgcgg caaggtaggc
    12061 ttcaaaacgc cctttgtttt caaagcggct gcggaagggg gaggttttga agtaatccaa
    12121 cattcgcggg ataatgccgc cgcacaaata cacgcccccg cgcgcgccca aagtcagggc
    12181 gaggttggaa gcgaccgtgc ccagcatcgc gcaaaaaatg tccaatgcct gccggcacag
    12241 gggcgattcg caatttaatg ccttttcggt gatttcagac ggcatcagtt ttgccggttc
    12301 ggctttctgc ttggccgcca gcgtttcata aatcaggctc aaacctgcgc cgctgaggaa
    12361 acgttcggcg gaaacgtggc ggtatttgtt tttggcgtac tgccaaatca acacttccat
    12421 atcgtcaaac ggcgggaaac tggtatgccc gccctcgccc gccaacgcca cccagcctgc
    12481 ggggctgtgc accaatccgc tcacgcccaa gccggtaccg gggccgataa cggccttggg
    12541 ggcgaattcg acaggctttt gcccgcctac ctgcatcagg tctttgcttg aagtctgcgt
    12601 taccgccaat gcctgcgcgg taaagtcgtt caaaaggatg agggtgtcca gccccaaagc
    12661 ctgacgggtg gtttcgatgg aaaacgccca atggtggttg gtcatctgca cccagtcgcc
    12721 caaaatcggg ttggcgatgg caaatgccgc gtgccgtacg cccgttgcac cactttggtt
    12781 cagataggca cgcaccgcgt cggtaaccgt gtcgtattcc ttacacggca ggacggcgac
    12841 tttctcaatc acgcacggcg cggtttccaa tgcgaaacgg gcgtttgtgc cgccgatgtc
    12901 ggcaaccagc ctcgggtaat cggcgtgttt attcggcgta gaagacatgg cagttcactc
    12961 cttgatggtt caaaacgagg ctgatcggat attcgcggtt ttcgccttgt gcggcttggt
    13021 cgaacacggc ttttttctct tcgccctgta tcgccaaaaa tacatgccca gtatgggcaa
    13081 tcgcatccaa agtcatgctg atgcgctcgt gcggcgcggt aacgggcgtg gtatgcacca
    13141 gcgcgacacc tgccgaaccg tcgattgccg tctgaaactg cggagctttt gggaaaatcg
    13201 aagccgtatg cccgtcgttt cccataccca aaatcaaaac atcgggctgc ttgtaatgtt
    13261 tcagcgcata atcgacaaca gcatcgggat gtaattcggt ttcagttttt ccgtcttcca
    13321 ccataggaat ccacactgcc gcagcggctt tgttctttaa caggtattcg cgcaccagac
    13381 cggtattgct gtcggcgtgg ttggtcggca cgatgcgttc atctgccaag gtaattccga
    13441 cgtttttcca atccaaatct ttttgcgaca gggcgttgaa aaatgcaatc ggcgaacgtc
    13501 cgccggaaac tgccaacacc gcaccgccct tctcgtccag tgcgccctgc aaagcatccg
    13561 ccactgcgtc agccaaagac tgcgccgctt ctgccgcatt ttcgtattcg tgccaaacaa
    13621 acatatttgt gtccttttgt gatttcagac ggcatatccc gttacagggc ggctgccggg
    13681 caatggctta gccgcttccg cctcgaagcg ggactgcttt ttctgcccgc cctccttcag
    13741 acggcattgc gaacccgatg ccgtctgaag cgcattactg ttcttcgtgc cacttgttgc
    13801 cgtcgcgcgc caacagttcg cgggcggctt cagggcccca agagtgtgcg ccgtaaccgt
    13861 gcggcggcgt ggtgttattt gcccagtttt ccaaaatcgg catcacatat tcccacgcgg
    13921 cttcgagttc gtcgcggcgg ttgaacaaag cgagtttgcc gttaatcaca tccaacagca
    13981 ggcgttcgta agcctcggcg cggcggcctt caacggcctt gcccaaatcc acgcccaaag
    14041 gcgttacttc cactttgttg cccgcacccg gggttttcac ctgcgtgtaa aggcggacgg
    14101 attcattcgg ttgaagctcg ataaccaaac ggttcggcgc ggtttggctg ttgtcgaaaa
    14161 tatggttttg cagcgggcgg aagttcaaaa cgatttccgc cactttgccc gccatgcgtt
    14221 tgccggtacg caggtagaag ggaacgccct tccagcgttc gttttcgatt tcggctttaa
    14281 tggcgacgta ggtttcggta aagctgtctt gcggaacgtt gatttcttca agatagccgt
    14341 tcatgccttt ggcagcggta tattgtccgc gcacgacatt ttcattgaca gattcgatgg
    14401 tcagcggctt caatgacttg atgactttga ctttttcatc gcgcaccgcg tcggcatcca
    14461 agctggcggg ggcttccatc gcagtcatgc acagcatctg catcaaatgg ttttgcacca
    14521 tatcgcgcaa cgcgccggta atgtcgtaaa actcgccgcg ctcttccaca ccgagctgtt
    14581 cggcgatggt cagctgcacg ctttcgatgt atttattgtt ccacagcggc tcgaacatta
    14641 cattggcaaa acgcagcgca agcaggtttt gcaggctttc tttgccaagg tagtggtcga
    14701 tgcggtaaat ttgcccttct ttgaaataac gcgccacatc ggtattgatt tgttgggaag
    14761 aagccaaatc cgtacccagc ggtttttcca aaaccacgcg cacattgtcg gcattcaaac
    14821 cgatcgcagc aaggttttcg caggcttgcg cgaagaattt cggcgcggtg gacagataga
    14881 tgacgacgtt gtcggtttct ttgcgtgctt tgaccaaatc gcccaaagcg gcaaaatcgt
    14941 ccggctgcgt aacatcgact ttgagatatg cgagacgttc aacaaacgaa gcccacgcct
    15001 caccggaaaa attttctttc acatggattt tggaactggt ttccaccttc gccagaaaac
    15061 cttcggtatc caactcgctg cggctgaccc ccaaaatacg cccttcggga tgaagcagac
    15121 cggcaacatg cgcctgatac aggcagggca aaagtttgcg cattgccaaa tcgccggtcg
    15181 caccgaacaa caccaaatca aaatttgtct gtgtactcat cgcattatct cgtcaggaaa
    15241 ggattttttc gatgccgtct gaaacctgtt ttttccatca cgcagcatcg caatatcgga
    15301 aacaaaggca ggcggcataa tgagtagtaa tactacacac cgctacactt tttgtctatt
    15361 cccattttta caatttattt gacctagtcc aaaaatcagg caggtttccc ctattccgtt
    15421 acaacaatca gaaaattctg cgatttaaat caaatttctt ttcaatgtct gattttttgt
    15481 aacaaaatt
//
LOCUS       DAXFZR010000055        14597 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000055 DAXFZR010000000
VERSION     DAXFZR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.055"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            168..2174
                     /locus_tag="ACFJAS_001473"
     CDS             168..2174
                     /locus_tag="ACFJAS_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC3982692.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2297..3389)
                     /gene="mltB"
                     /locus_tag="ACFJAS_001474"
                     /pseudo
     CDS             complement(2297..3389)
                     /gene="mltB"
                     /locus_tag="ACFJAS_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
     gene            complement(3537..4964)
                     /locus_tag="ACFJAS_001475"
     CDS             complement(3537..4964)
                     /locus_tag="ACFJAS_001475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC3982693.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5149..8853
                     /gene="mfd"
                     /locus_tag="ACFJAS_001476"
     CDS             5149..8853
                     /gene="mfd"
                     /locus_tag="ACFJAS_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC3982694.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDDPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQKINAIHEELVDRFGLTEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9079..9381
                     /locus_tag="ACFJAS_001477"
     CDS             9079..9381
                     /locus_tag="ACFJAS_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFC3982695.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            9543..9926
                     /gene="panD"
                     /locus_tag="ACFJAS_001478"
     CDS             9543..9926
                     /gene="panD"
                     /locus_tag="ACFJAS_001478"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC3982696.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            9948..10790
                     /gene="kdsA"
                     /locus_tag="ACFJAS_001479"
     CDS             9948..10790
                     /gene="kdsA"
                     /locus_tag="ACFJAS_001479"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC3982697.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            10805..11245
                     /locus_tag="ACFJAS_001480"
     CDS             10805..11245
                     /locus_tag="ACFJAS_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC3982698.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11292..12578
                     /gene="eno"
                     /locus_tag="ACFJAS_001481"
     CDS             11292..12578
                     /gene="eno"
                     /locus_tag="ACFJAS_001481"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC3982699.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            12746..13024
                     /gene="ftsB"
                     /locus_tag="ACFJAS_001482"
     CDS             12746..13024
                     /gene="ftsB"
                     /locus_tag="ACFJAS_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC3982700.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13065..13355)
                     /gene="yfaE"
                     /locus_tag="ACFJAS_001483"
     CDS             complement(13065..13355)
                     /gene="yfaE"
                     /locus_tag="ACFJAS_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC3982701.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13476..>14597)
                     /gene="nrdB"
                     /locus_tag="ACFJAS_001484"
     CDS             complement(13476..>14597)
                     /gene="nrdB"
                     /locus_tag="ACFJAS_001484"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC3982702.1"
                     /translation="TFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQLSFF
                     WRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSIPELE
                     TWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDDLIEY
                     TQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFAFAER
                     ELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQLFKKA
                     AEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLSAGFEGANQNPIPWIN
                     AWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
BASE COUNT         3509 a         4275 c         3623 g         3190 t
ORIGIN      
        1 tcgaaaaaat taaaaatcaa tacggattat tctatcagat tgatttatcg atatttatta
       61 tatcattaat atagtttgat ttcaaaccgg cggaaaatgc gccgcccgcc gcagatgcgc
      121 cctccccgcc atcggggcgt ttaacgccgg attgccgatg cggtacaatg gcagatatga
      181 agaaaattac ccctcaaaac ctgcgtcccc tgctttcgga aagcttggga cataccgatt
      241 ttgtcaacgt cctcaacgca ttgattaaat ttttgcgccg tggcggcaaa aaatgtgcgg
      301 gggagcgttt cgacctgatt atcgacacat tcaaacaaga cagggaatta ctgtcccgct
      361 tcagccggtg tttttacatc tggctcgcgc aaatacacat ttatccggca ctcatcaaac
      421 tcggcatttt ctcgcgccac agctttgccc gggaaatggg catacgcata tacgaacgct
      481 tcaacccgtc atataaagat tttgccaact tgggcgaagt cttcctttat cttttccatt
      541 ccgaaaacga cgacaaatgg ctgcaaacgc tcaatatccg ccaatggctg gttttatacg
      601 aactcatccg tagccacgcc gagccgtcca aattgcagac ggcgggcatc cgccttgccg
      661 atgcgcgttt gcgcgccatc gaaatgctgt ctgtctggac ggcatccgaa gccatcgaac
      721 ccgatctcat ccgtatcgcc ccgcgcctgc tggaagccga ttcttccttc gtcgccctcc
      781 aacgcgaaac cgccaaactg gtcgaacact accgcaacgg caccgcgcct tacgacaccg
      841 cccacctcga agtgatgttc gaccaatgtt tcagccagat tgactatttg cgtcgcaaag
      901 ggacgggcgc cggctccggt tcgtcggtca aagtcgccca cctgctcgaa cgactccggc
      961 agaccgtagg ccgtctgaag ctgctcacag acatacaaac cggtgccggc aacagcaacc
     1021 gcctgaccat cgccctgatg aactccctca tctacgcggc ggtcgaacaa tacagcaccc
     1081 gccacctgcg ccgcagcagc atccgtatgc tcgcccgcag cattaccgaa aacaaaagcc
     1141 accacggcga acactacatc acccgcaacc gcaaagaata tttcaaaatg ttctactcgg
     1201 cagcaggcgg cggcggcatc atcgccctga tggcgctgct caaaatccgc atcggcacac
     1261 tcgacctcag tcccttcctc gcttccttgt cggctgggtt caactacggc atcggcttta
     1321 tgatcatcca tatgctgcac tgcaccgtcg ccaccaagca gcccgcgatg actgccgcca
     1381 gctttgccga acaggtcgat ctcaacgaag gcggcaaagc ggtggacaac aaactctcca
     1441 agctcctcat cgacgtatgc cgctcccaaa gcgtcgccgt cttcggcaac gtttccatcg
     1501 ccatcctttt ggcgtgcgcc atatcgttcg gctatgccca tctgtaccgg ctgcccatac
     1561 tcgatgccca caccgccgcc taccagttca aatccataga catcatcaat catcctaccc
     1621 tatggtatgc cgctattgca ggcttgtggc tgttctgctc cggcatcatc gcaggttttt
     1681 tcgacaaccg cgccgactac ctcaacctgc gccaacgcct gcccttcaat cccttgctgc
     1741 gtaaaatcat gcgccccggg ccccgccgcg tcctcgccgc ctacatccac aaacactacg
     1801 gctcgctggt cggcaacttc atcttcggaa tgctcttggg tatgaccggc tatttcggac
     1861 acctcctcgg gctgccgctg gacatccgcc acgtcgcctt ttcctccgcc aacctcggct
     1921 atgccgccgt cagcggcaac gtcggctttg gtacattcgt actcggcatt ttcagcgtcc
     1981 tcgccatcgg cttggtcaac ctctgcgtca gcttcagcct cgccctcttc gtcgccctgc
     2041 gctcgcgcgg cacaaaaatc ggcagcatcc gcaacctgat caaaagtttt tggaatcaga
     2101 ttaaaagcaa tccttgcata cttttcctcc cgcccgccaa aggacaggga catcctcctt
     2161 cggacaagcc ttgaccggca atgccgtctg aaacgggatt cgccccgaat accgccctga
     2221 tgcgggaaat ccccataaaa ggatgcaaaa atgccgtctg aaccgaaacg tggttcagac
     2281 agcattttaa aaaacattac aatcccgggc cgccgagcga attggcaatg tccctgaccg
     2341 ccgttacata catccggctg tggttgtact gccataccgt ataaaaattg ttcaagccca
     2401 aataatattc aaacacgccg ggtgcggttt ccagtttgaa caaaaccgcc ttttcatcat
     2461 ctgcgagcgt ttccccgggg atgatgccgt acgccttcaa atccgccacc gtccgcgtca
     2521 gggcggtttt ttcgccaatg attgcctgaa catccgcacc cggcgccaac gtcgccgaca
     2581 ccaacatttt accgcccgtg cgccaaccgt gctgcttcat ataattggca accgatgccg
     2641 cgacatcacc gacgttgccc catatatccc gatgtccgtc cccgtcataa tccaccgccc
     2701 atttccggta gctcgaaggc ataaattgcg gcatacccat tgcgcccgca tagctgccct
     2761 taaaggcgaa aacatcgccg ccttcttctt ttgccagctt taaaagctcg accaattctt
     2821 tttggaaaaa cccggcgcgg cggggataat caaagcctaa ggtcgccaat gcgtccgcca
     2881 cacggaagct gcccgtattt ttgccgtaat tcgtttcaat cccgataatc gccacgataa
     2941 gctcggcagg cacgccgtat ttttgcgcca catcatcgat aaccgcgcgg ttttccgcat
     3001 aaaacctgcg cgcgccgtga aatttcgccc tgcccgaatt tcccgtgcgg aacacatacc
     3061 acggacgcga tgtggagggt cggtgcataa tcttgacgat gtccgccttg taagccgctt
     3121 tgtcaaaaaa atcctgccat tccgcctggg aaaaatcccc tttcccgact tcatcgtcca
     3181 caaaacggcg gacatttgca ttggcggcaa acccgctgtc ggataccggt acggctgccg
     3241 cgtcaaacgc ggggcggctt tcttttttca tttcatccgc gcgggggggc ttgggcttca
     3301 tttgcccggg gtgtgcgggc ctccatcgcc gtacaggcag acaaagccgc caaacaaatt
     3361 gccagcggca gtatttttct cttttccata aacacattcc gaacaaatag ggtaagtggg
     3421 aaagcggcac aagggtgagg cgcgaaatgc ggtatccgcc gcaatcggcg gttttgcaga
     3481 atgccgcgcg ttgccttttg cgccgcccga aatctgcctg ccgccgtatt tgcccgctat
     3541 ccgcaatatc ggcagtccaa tatatctttg cggatgtcgt tcagcaggaa ggctgtggtg
     3601 tcttcgtgtt cctcctgtac gaagacaaag agtcgggcga tgattttgcc gatgaagact
     3661 ttttgcaggg cgcaggcgtc ggtcagggtg tgttcctgca ggaagctgcg gatgtcttcg
     3721 ggcaaagcgt agtcgcgggc aacggcggca aagcggacat cggtttgcag gcggtcgagc
     3781 agggtttggt ttttgtccaa cttaatgcct gcttcttttt cttcggcggt ggcgcggacg
     3841 aactggtcgt aaatttcggc ataaagcata tcgttcgggc cttcaaaaat cgtgaagggg
     3901 cggatatcga tggcgatatt gccggctgga tgtccgcgtt caaaaccctt cgcgcccaag
     3961 agtttttgca acatttgcgc ggcggcgtaa gtgtattccg tggcgagggt tttgacgatg
     4021 ttcgcctcca tcaattgatg ggcgacgggc gcgacgggcg aaacggaatg gcagacgtag
     4081 cggtaaagga tttcggaaac ctgatggcgg cgttggattt cgcggcgttc gtaatcgacg
     4141 aatctgatat cgttgcggac atatcggttc aggttgtcaa ggatgtattc cataatgccg
     4201 tgcgtcatgc cgatcagttg caggcggctg cggataaaga tgttttggaa cgcgcgcaaa
     4261 ccggcagcgt cgccccggga gagtttcatc acggcggttg caggcatttc ggcatcgatg
     4321 cggttgacgg cgtaacggac ggcgcgcagg ccttcggatg cgagggtttc gcagcggatg
     4381 tatgttttgg ggacgagcag caggtcgatg actttggcga gtttgccgtt tttgcgctct
     4441 ttggcggcaa cgaggaggaa gtcgctttgc gaattgccct gccagtattt cgcggcgttg
     4501 acgtaaatgg tttgttcgtc ggtatattcg tagcaggact gcatttcgcg tgcaatcgcc
     4561 gcgccggagg tttcgggttc ggtaacgccc aaaccgccgc cttcgccttt gaaaatcatg
     4621 tccaaacctt gtgcgacttg cgcttcgccg ccgaactctt gcagaggctg caacaccagc
     4681 gcgccttcga tgccggtacg cagcgtaacg ggcacgccgt aatgccccgc aatccttagg
     4741 acttcttgga tttcaaactg actgcccttg cgcccgccgt gttttttgtc gaggaagggc
     4801 aacagcaaac ccgcctgctt caaggcaagc catttgtctt cgggcaggta tcgcatcagg
     4861 tcgatgccgt ctgaaaaaat gcggcggaat gcggattcga tgtgctttaa aaaagcagcc
     4921 gtgtccatgg ttgacggctg cgcgctcggt tcggtgtgta tcatcggctt cctctgtcgg
     4981 ttcccattaa tcggcggccg gtcaaaccgc ctgccacagt ttagagttaa ttttctaaac
     5041 tttaccacaa agtgcaccgg gcaacaatcc gccggccttt cagacggcat cgcgcaccct
     5101 cacgtgctaa aatgaccgtt tgcatcactg tccgccgatt gccgcactat gacctgcccc
     5161 atccccaaac cccgtgaaaa atcccgttgg ttcaatcttt cgcaaggctc gctgcccttg
     5221 gctttggcgc gttatttgcc gcacaagcgg ctcaaagccg tgctgaccca agatgcggaa
     5281 caggcgttgc gccttcagac ggcatggcgg tttttccgtc cgcacgacac ggcggtgttc
     5341 ctgccggact gggaaacgct gccttacgag cgtttttctc cgcatcagga tttggtgtcg
     5401 gagcggctgt cggcgttgtg gcagattaaa agcggcgcgg cggacgtgtt gttcgtaccg
     5461 gttgccacgg cgatgcagaa gctgccgccc gtgccgtttt tggcggggcg cacgttttgg
     5521 ctgaaaacgg ggcagacttt ggatataggc cgcctgaaaa ccgatttggt ggatgcgggc
     5581 tacaatcatg tttcccacgt tgtcgcggcg ggcgagtttg ccgtgcgcgg cggcattgtc
     5641 gatttgttcc cgatgggcag cgaaacgccg taccgcatcg atttgtttga cgatgaaatc
     5701 gacagcatta aaaccttcga taccgacacg cagcgcacca tttcccccgt ttccgaaatc
     5761 cgcctgctgc cggcgcacga gttccccacc gacagcgagg cgcaaaaaat cttccgcagc
     5821 cgcttccgcg aggaagtcga cggtaatccg aacgatgccg ccgtgtacaa agccgtcagc
     5881 aacggccact tcggcgcggg cgtggaatat tatctgccgc tgttttttga aaacgagctg
     5941 gaaacgctgt ttgactatat cggcgaagat gcgctgtttg tctctttggg cgatgttcat
     6001 gccgaggcaa accgcttttg gaacgacgtt aaatcacgtt acgccatggc gcagggcgac
     6061 gaaacctatc cgcctttgct tccacagcat ttgtatctct ctgccgatgt gttcgcaggc
     6121 cgtctgaaaa actacggaca agttctgcct gatgtttccg gcaaggcaca ctccctgccc
     6181 gaccttgccg tcaaccgcca atccgacgat cctttgcagg cattgaagga ttttcagacg
     6241 gcctttgacg ggcggatttt gctgtgcgcc gaaagtttgg gacggcgcga aaccatgctc
     6301 ggtttcttgc agcaaaacgg tttgaaagcc aaacctgtgt ccgactggca gggcttcttg
     6361 tcggcgcacg agccgctgat gattacggtc gcgccgttgg cttacggatt caaactgggc
     6421 ggactgcaat cttcaagcca acagcaaact gttcccgcct ccgagggaga aggcaaagcg
     6481 gttaccgacc aaaccgaatt ttccgcatcc gcaacaaacc ctctccccag ccctctcccg
     6541 caggagaggg aacaaagtgc cgccgccgtt tcagacggcc tgaaagcagc cgccgtttca
     6601 accgaaagca gcctgtatct cgtcgcaagt gatctgcacg ggcaaacccg acagcaatct
     6661 gccccctccc ccgtggggga gggttgggga gagggcaaag cggttgccgc tcaaagtgcc
     6721 atcgccgtca tcaccgaatc cgatctttac caatacgtcg cccgttcgcg cgtccacaac
     6781 cgccgtaaga aacacgccgc cgtttcagac ggcctgttgc gcgaccttgc cgaaatcaat
     6841 atcggcgatc ccgtcgtgca cgaagaacac ggcatcgggc ggtatacggg cttggtaacg
     6901 atggatttag gcggcgaaac caacgaaatg atgttgctcg aatacgcggg cgaagcgcag
     6961 ctttatgtgc ctgtttcgca actgcattta atcagccgct actccggtca ggcgcatgaa
     7021 agcgtcgccc tgcacaagct cggcagcggc gcgtggaaca aggcgaagcg caaagccgcc
     7081 gaaaaagcgc gcgataccgc cgccgagttg ctcaacctct acgcccaacg cgccgcccaa
     7141 tcgggacaca agtttgaaat caacgagtcg gactatcagg cgtttgccga cggcttcggc
     7201 tacgaggaaa ccgaagacca ggccgccgcc atcgccgccg tgattaaaga tttgacgcag
     7261 gcgaagccga tggaccgact tgtgtgcggc gatgtcggtt tcggcaaaac cgaagtcgcc
     7321 cttcgcgccg cgtttgtggc ggtgatgggc ggcaaacagg tcgccgtact cgccccgacc
     7381 acgcttctgg tcgagcagca cgcgcaaaac ttcgccgacc gtttcgccga tttccccgtg
     7441 aaagtcgcca gcctttcgcg tttcaacaac agcaaagcca ccaaagccac gctcgaaggc
     7501 atggcggacg gtactgtcga tatcgttatc ggtacgcaca aattggtgca ggacgacata
     7561 aaattcaaaa acttaggttt attgattatc gatgaagaac accgcttcgg cgtgcgacag
     7621 aaagagcagc tcaaacgcct gcgcgccaat gtcgacatcc taaccatgac cgccacgccg
     7681 attccgcgca ccctcagcat ggcgctcgaa ggtctgcgcg acttctcgct gattaccact
     7741 gcaccgagcc gccgcctcgc cgtgaaaacc tttgtcaaac cgttcagcga aggcagcgtg
     7801 cgcgaagccg tgttgcgcga actcaaacgc ggaggacagg tatttttcct gcacaatgaa
     7861 gtagatacga ttgagaatat gcgcgagcgg ctggaaaccc tgctgcccga agcccgtatc
     7921 ggcgtggcgc acggacaact gcgtgagcgc gagctggaac aagtcatgcg cgactttttg
     7981 cagcaaagat tcaatgtgtt gctctgttcc accatcatcg aaaccggcat cgacatcccc
     8041 aacgccaaca ccatcatcat caaccgcgcc gacaaattcg ggctggccca actgcaccag
     8101 cttcgcggac gcgtcggccg cagccaccac caagcctacg cctacctgct cacgcccgaa
     8161 tacatcacca aagacgcaga aaaacgcctc gacgccatcg cggcggcaga cgaactcggc
     8221 gcaggtttca ccctcgccat gcaggatttg gaaatccgcg gcgcaggcga aatcctcggc
     8281 gaagggcagt ccggcgaaat gatgcaggtc ggcttcacgc tctacaccga aatgctcaaa
     8341 caggccgtgc gcgacctcaa aaaaggccgc caacccgacc tcgacgcacc gttgggcatt
     8401 accaccgaaa tcaaactgca cagcccggca ttactgcctg aagactactg ccccgacatc
     8461 cacgagcgcc tcgtcctcta caaacgcctc gccgtatgcg aaaccgtgca gaaaatcaac
     8521 gccatacacg aagaactcgt cgaccgcttc ggcctgaccg aacaacccgt caaaaccctc
     8581 attgaaagcc accacctgcg gcttgcggca aaagaactgg gtattgatgc catcgatgcc
     8641 acaagtgaag ccgtaaccgt taccttcggc aaacaccact gtatcgaccc gacggggatt
     8701 atcctgctga ttcagacgga taaaaaatac cggctggcag gggcagacaa attgaggttt
     8761 gcagcggaaa tggaaaacat cgaggtcagg atcaataccg tcaaaaccgt actaaaaacc
     8821 ctgcaaggca aacgcctgcc caaaggaaat tgatgtaacg atgccgtctg aagaacaccg
     8881 ttcagacggc atacaatccc ccaatcctca ccaaaaggaa caatcatgtc gatttttcac
     8941 ccctatttcc aacaactgtc caccgaaggt tttgacggag aagaagatgt cctctgggaa
     9001 gacgagctga ccgtcaaagg caatcttgtc gaggtattcc tgtaggcaga aaatgaaaac
     9061 agcctgccaa ccgaaacatt ggatgcattt gccgccttcc tgtccgaatt ggaaaaagcc
     9121 gacgatgctg cacgcgccgc actgacggaa tatttgaaac gggacagccg ctatatcgac
     9181 tttcacaccg aagataccca aacaagccgt gatgcggcaa aatttgtccg cactatgcag
     9241 ttgatctata tcagcttatg ggcaaaaaat ccggcttttg ccgttatgga ttatatgccc
     9301 gccaatatgg aaagcgacga aatccttgcc gtcaaactgc atttggacgg cagcattttc
     9361 tcaatcgatt gggagagttg agccggattg ccaatcggca aaaatgaaat gccgtctgaa
     9421 aacagcttcc ctgcccgaag ccttgccatt atgaatttga aggaaactcc actataatac
     9481 ggcattcaga ttttcagacg gcatcacgcc cgtcagaccg cacacaaacc aaaaggaaat
     9541 acatgttccg tactatactt ggcggaaaaa tccaccgcgc caccgtaacc gaagccgatt
     9601 taaactacgt cggcagcatt accgtcgatc aagacctgtt agacgcggca ggcatctgcc
     9661 ccaacgaaaa agtcgccatc gtcaacaaca acaacggcga acgttttgaa acctatacca
     9721 ttgcagggaa acgcggcagc ggcgtgattt gcctgaacgg tgctgcagcc aggctggtac
     9781 agaaaggcga catcgtcatc attatgtctt atatccaact ttccgaaccg gaaatcgccg
     9841 cacacgaacc caaagtcgtc ttagtggacg gaaacaataa aatccgcgac atcatctcct
     9901 acgagccgcc gcacaccgta ctgtaatccc gcaaacggac atcgattatg gatattaaaa
     9961 tcaacgacat caccctcggc aacaattcgc ctttcgtcct attcggcggc atcaacgttt
    10021 tagaagattt ggattccacc ctccaaacct gtgcgcatta cgtcgaagtt acccgcaaac
    10081 tgggcatccc ctatatcttt aaagcctctt tcgacaaggc aaaccgctcg tctatccatt
    10141 cctatcgcgg cgtaggcttg gaagaaggct taaagatttt tgaaaaagtc aaagcagagt
    10201 tcggcatccc cgtcattacc gacgtacacg aaccccatca atgccaaccc gtcgccgaag
    10261 tgtgcgatgt catccagctt cccgcctttc ttgcgcggca gaccgatttg gtggccgcaa
    10321 tggcggaaac gggcaatgtt atcaacatca aaaaacccca gttcctcagc ccttcgcaaa
    10381 tgaaaaacat cgtggaaaaa ttccgcgaag ccggcaacgg gaagctgatt ttatgcgaac
    10441 gcggcagcag cttcggctac gacaacctcg ttgtcgatat gctcggtttc ggcgtgatga
    10501 aacaaacctg cggcaacctg ccggttattt tcgacgttac ccattccctg caaacccgcg
    10561 atgccggttc tgccgcatcc ggcggtcgtc gcgcacaggc tttggatttg gcacttgcag
    10621 gcatggcaac ccgccttgcc ggcctgttcc tcgaatcgca ccccgatccg aaactggcaa
    10681 aatgcgacgg ccccagcgcg ctgccgctac accttttaga aaatttttta atccgcatca
    10741 aagcattgga cgatttaatc aaatcacaac cgattttaac aatcgagtaa cacggtttcg
    10801 ccttatgatg cagactttcc gaaaaatcag cctgtatgcc gcaaccttgt ggctcggtat
    10861 gcagattatg gcaggttata tcgccgcacc ggtgctgttc aaaatgctgc ccaaaatgca
    10921 ggcgggcgaa attgccggcg tattgttcga catcctctct tggagcgggc ttgccgtttg
    10981 gggcacggta ctggctgccg cctttgccgc cctaacccgg cggcaaaccg ccctgctgct
    11041 ttttttattg tccgcccttg ccgccaacca atttttggtt acacccgtta tcgaggcact
    11101 gaaatacggg catgaaaatt ggctgttgtc ggttgcaggc ggatccttcg gaatgtggca
    11161 cggtatttcc agcatgactt tcatggcaac cgccctactt tcagcagttt taagttggcg
    11221 gctttccggc aaagaggccg tctgaagccc tcccattttt tacctccctt cacttcactt
    11281 ggagaacatt catgagcgca atcgttgata ttttcgcccg cgaaattttg gactcgcgcg
    11341 gcaaccccac agtcgagtgt gatgtattgc tcgaatccgg cgtaatggga cgtgcggccg
    11401 taccgagcgg cgcatccacc ggtcagaaag aagctttgga acttcgcgac ggcgacaaat
    11461 cccgctattc cggcaaaggc gtattgaagg cggtcgaaca cgtcaacaac caaatcgccc
    11521 aagccctcat cggtatcgat gccaacgagc aatcttatat cgaccaaatc atgatcgaat
    11581 tggacggtac tgaaaacaaa ggcaatttgg gtgcgaatgc gactttggcg gtctctatgg
    11641 cggttgcacg cgccgctgcc gaagactcag gcctgccgct ttaccgctac ttgggcggcg
    11701 caggtccgat gtccctgccc gtaccgatga tgaacgtcat caacggcggc gaacacgcca
    11761 acaacagcct gaacatccaa gagtttatga ttatgcccgt cggcgcaaaa tctttccgcg
    11821 aagcgttgcg ctgcggtgcg gaaattttcc acgccttgaa aaaactgtgc gacagtaaag
    11881 gcttcccgac cacagtcggc gacgaaggcg gtttcgcccc caacctgaac agccacaaag
    11941 aagccctgca actgatggtc gaagcggccg aagccgccgg ctacaaggcg ggcgaagacg
    12001 tattattcgc attggactgc gcgtccagcg agttctacaa agacggcaaa taccacttgg
    12061 aagccgaagg ccgctcctac accaacgcgg aatttgccga atacttggaa ggcttggtta
    12121 acgaattccc gattatttcc attgaagacg ggatggacga aaacgactgg gaaggctgga
    12181 aactgctgac cgaaaaattg ggcaaaaaag ttcaattggt cggcgacgac ttgttcgtaa
    12241 ccaatccgaa aattcttgcc gaaggcatcg aaaaaggcgt agcaaacgca ttgctggtca
    12301 aagtcaacca aatcggtact ttaagcgaaa ccctgaaagc cgtcgatctg gcaaaatgca
    12361 accgctacgc cagcgtgatg agccaccgct ccggcgaaac cgaagacagt accattgccg
    12421 acttggcagt cgccaccaac tgtatgcaga ttaaaaccgg ttctttgagc cgttccgacc
    12481 gcatggcgaa atacaaccaa ctgctgcgta tcgaggaaga attggcggaa gccgcctact
    12541 accccggcaa agccgcattc taccaactgg gcaaataaat aaaaaagtgg atgtatgttt
    12601 caacacacag ggcaacacat gaagcgtcgc cctgtgtgtt gacctgattt ggaagaggtt
    12661 acacctctcc caaataaagt ctaattttac cgccacgaag gacggacatc cgagtggtgg
    12721 ggtttcaacc agtaaggaaa cgttgatgaa gtgggtaact gtcgttttat ccttcgcact
    12781 tgtctgttgc caatacagcc tgtggttcgg caaaggcagc atcggacgca acagcagtct
    12841 gagagaacag attgccgttc aagaagaaaa aaaccagaca ctcgccctac gcaatcattc
    12901 ccttgccgcc gaagtctatg atttggaaaa cggtcaagaa gccatttcgg aaatcgcccg
    12961 ggtagaactg ggttatatcc aagacggcga aaccttttac cgactcatca ggcataaccg
    13021 gtaataccgt caaaaagccg tccgaaccaa tgttcggacg gcttttattt caacaaactg
    13081 tcagacagcc cctcatcctc ccccgccaaa ccgcaatcca gcctgacatc cccctcgacg
    13141 caacagcagc acggcagtat ctcgtcccgc cccaaaaaag ccaaaggcgg ctcccgataa
    13201 gtaacgcttc cctccaaaat ctttacccgg cacgatccgc aatatccgct tcggcactga
    13261 tattccacca tatgccccgt ccgttccaag ccttccaaca gggtctcacc ctccaagagc
    13321 tcaaaaaggc ccttattcgt accaatgcgc gccatttccg accaatcaaa aataaaagga
    13381 acaataaccg atcctacctg ctgtttttcc catcatacaa cacacaaatg ccgtctgaaa
    13441 catccggctt cagacggcat tttttcaaaa aacatttaca actcaaaatc gcccaagtca
    13501 tccgtattca cttcagaatc gatttgacca atcaaataag aggatatttc cacttcctgc
    13561 ggcgcgacct gtacgttgtc ggacgacagc cacgcattaa tccacggaat cgggttttga
    13621 tttgcgcctt caaatccggc cgacagcccc accgcctgca tacgcagatt ggtaatatat
    13681 tcgacgtatt gggataagat ttctttgttc aaaccgatca tcgaaccgtc tttaaacaaa
    13741 tatgccgccc attctttttc ctgttccgcc gcttttttga agagttggaa acattcgtcc
    13801 tgcaactcgg cggcaatttc cgccatttcc ggatcatcaa cacccgaacg catcagatta
    13861 agcatatgct gcgtgccggt caggtgcagg gcttcatcgc gggcaatcag tttgatgatt
    13921 ttggcgttgc cttccatcaa ctcacgctca gcaaaagcaa acgagcaggc aaatgaaacg
    13981 tagaaacgga tggcttccaa cacattgacg cacatcaggc agagatagag ttttttcttc
    14041 aacccgcgca aagacacagt aacgggtttt ccgccgacat tgtgcacccc ttcgcccaac
    14101 aggttgtaat attgggtgta ttcgattaaa tcatcataat agcaggcaat gtcttcggcg
    14161 cgggcggtaa tgtattcgtt ttcgacaata tcatcaaaca cgaccgacgg atcattcaca
    14221 atattgcgga tgatgtgggt atagctgcgc gagtggatgg tttcgctgaa gctccacgtt
    14281 tcaacccaag tttccagctc gggaatcgaa accaaaggca gcaaggcaac attcggactg
    14341 cgcccttgga tggaatcgag cagtgtttga tatttcagat tgctgatgaa aatatgtttt
    14401 tcgtgttcgg gcaggttggc gtagtcgata cggtcgcgcg acacgtcgat ttcttccggc
    14461 cgccaaaaaa acgacaattg tttttcaatc agtttttcaa atacctcgta tttctgctgg
    14521 tcataacggg cgacatttac cggctgaccg aaaaacatcg gctctttcag cgcgtcgttt
    14581 ttggttttgg gaaaggt
//
LOCUS       DAXFZR010000056        14282 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000056 DAXFZR010000000
VERSION     DAXFZR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14282
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.056"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(196..528)
                     /locus_tag="ACFJAS_001485"
     CDS             complement(196..528)
                     /locus_tag="ACFJAS_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982703.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            714..1543
                     /gene="cysT"
                     /locus_tag="ACFJAS_001486"
                     /pseudo
     CDS             714..1543
                     /gene="cysT"
                     /locus_tag="ACFJAS_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
     gene            1732..2553
                     /gene="cysW"
                     /locus_tag="ACFJAS_001487"
     CDS             1732..2553
                     /gene="cysW"
                     /locus_tag="ACFJAS_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC3982704.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIAVPVNAVLGVAMAWLLTRFDFLGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGSRLEAQGIQIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQTQGDSEEQAALVLGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITKL"
     gene            2549..2656
                     /locus_tag="ACFJAS_001488"
                     /pseudo
     CDS             2549..2656
                     /locus_tag="ACFJAS_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2694..3770
                     /locus_tag="ACFJAS_001489"
     CDS             2694..3770
                     /locus_tag="ACFJAS_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC3982705.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPADKLVSLLGPSGGGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGEADAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IRPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
     gene            complement(3826..5352)
                     /gene="ilvA"
                     /locus_tag="ACFJAS_001490"
     CDS             complement(3826..5352)
                     /gene="ilvA"
                     /locus_tag="ACFJAS_001490"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC3982706.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGMAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGGDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            5501..6670
                     /locus_tag="ACFJAS_001491"
     CDS             5501..6670
                     /locus_tag="ACFJAS_001491"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC3982707.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLSLLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            complement(6796..7368)
                     /locus_tag="ACFJAS_001492"
     CDS             complement(6796..7368)
                     /locus_tag="ACFJAS_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC3982708.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(7435..8418)
                     /locus_tag="ACFJAS_001493"
     CDS             complement(7435..8418)
                     /locus_tag="ACFJAS_001493"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC3982709.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGKTC
                     LIVDDMIDTANTLCKAAVALKERGAEGVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(8586..8661)
                     /locus_tag="ACFJAS_001494"
     tRNA            complement(8586..8661)
                     /locus_tag="ACFJAS_001494"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8626..8628),aa:Gln,seq:ttg)
     gene            complement(8674..8749)
                     /locus_tag="ACFJAS_001495"
     tRNA            complement(8674..8749)
                     /locus_tag="ACFJAS_001495"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8714..8716),aa:Gln,seq:ttg)
     gene            complement(8757..9602)
                     /gene="ispE"
                     /locus_tag="ACFJAS_001496"
     CDS             complement(8757..9602)
                     /gene="ispE"
                     /locus_tag="ACFJAS_001496"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC3982710.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWRLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(9612..10193)
                     /locus_tag="ACFJAS_001497"
     CDS             complement(9612..10193)
                     /locus_tag="ACFJAS_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC3982711.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGGTLGQLCQDRDGALAVDGKGNVYQAEGTE
                     DLSRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(10333..12171)
                     /locus_tag="ACFJAS_001498"
     CDS             complement(10333..12171)
                     /locus_tag="ACFJAS_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC3982712.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            complement(12498..13334)
                     /gene="panC"
                     /locus_tag="ACFJAS_001499"
     CDS             complement(12498..13334)
                     /gene="panC"
                     /locus_tag="ACFJAS_001499"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC3982713.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            complement(13457..14248)
                     /gene="panB"
                     /locus_tag="ACFJAS_001500"
     CDS             complement(13457..14248)
                     /gene="panB"
                     /locus_tag="ACFJAS_001500"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC3982714.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKVHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
BASE COUNT         3362 a         4117 c         3465 g         3338 t
ORIGIN      
        1 gcaacaaacg caaatcaatg ctttgcgttt gttgcagtcc caaaataatt gatgctgcct
       61 tattataata ccaagataat ttttattcaa taaaatacaa agggaagcgt tccgcccatt
      121 gcaaacagat gcaatccgcc gattatttaa aaaacggcaa agccttgcac cccttgcggc
      181 aagcctgcca tgcctttaat gtccgcaggc gcaagcgtcg ccgtggtgtc cgcaatcttt
      241 agccgcttcg agccacgcgt cgtcatcgcc gtcaaactct tccacttcgt caaactcgcc
      301 gcgttccacc atcgcgcaca actcttcaag gctttccgcc ccttccaccc aaaaacaggg
      361 aatgcccggc ggcgtatcgg gcgcaagcag tgccgccacg ccttcatgcc acgcaatcca
      421 atagccgttg tccgtacgga caaattgtgc gttttcgttg accgactcgc cttccgtacc
      481 ttccagcata cgttccgcaa tgtccgcctg aaaagataaa atctgcataa gtgttccttt
      541 atatgatggt tttccgtcaa aacaaagcgt tattttagaa catcgcgccc gcttgaaaaa
      601 ctgccaaccg ctttataaca aattcgtctt tgcaccaaac tttccatttt ttccgttttt
      661 caggcggcat tgttaaagta gtccttcctt ttccttattt tcagcattgt tttatgttac
      721 cgctcaaaac acccggcgta ctgcccggct tcaaactcag cctcggtctg accgtattgt
      781 gcctgtcgct tctggtggtt ttgccgtttg cgatgatggc ggcggaaatc ggctggggcg
      841 gctttcggaa cacgattgcc gagccgaacg tgctggcggc ggtgcggctg agtttgcgga
      901 tgtcgtttta tgcgatgctg accaatgtgt cgtgttcggc acactggtgg cttgggtgtt
      961 ggtgcgctat gaatttccgg gcaagggcct ggcgaacgcg ccggttgatt tgccgtttgc
     1021 gctgccgacg gcggttacgg gtatcgcgtt ggcggccctg tatgcgccca acggttggat
     1081 aggccgtttt ttcgaacctt tgggcatcaa aatcgcgttt acgcccgtcg gcatttggat
     1141 tgcgctggtt gtcgtcagcc tgccctttat cgtccgcgcc gtgcagccgg tattgaagga
     1201 attgtcgggc gaatatgagg aagcggcggc gactttgggc gcaaaccgtt tcacgacgtt
     1261 ccgccgcgtg ctgctgccgg aaatcacgcc ggcgctcttg accggcgcgg gcatgatgtt
     1321 cgcgcgggca acgggggaat acggttcggt gatttttatc gcgggcagca ttccgatgat
     1381 ttccgaaatc ctgccgctga ttattacggg caagctggaa caattcgacg cgcagggcgc
     1441 gtcggcggtg gcgttgttta tgctgctggt ttcgtttgtg attctgtttg cgctgaacgt
     1501 gatgcagtgg gcgttgggca ggcgttcggg tgcgaagggt tgaggtcgtc tgaaaacgaa
     1561 tccgctcccc aaaacacaga attcgtaggt cggatacttg tatccgacaa acatttgcga
     1621 ttcagcaacc ttgtcggatt caagaatccg accgacgaaa gctgaaaccc ctcaatccct
     1681 ttcagacggc atccgaacac cttaaacaac aaaccagaga acaccaccgc catgaaaccc
     1741 tattccgcca atcccaacct gaccgaaccg cgccggctgc gcatgttgct gattgccgcc
     1801 gcgctgggct ttctgctgct gatgctggtc gtgccgctcg tcgccgtatt ctacgaagcc
     1861 ttaaaaggcg gttgggattt gtacctgaaa tccttaagcg atcccgaagc gtggtctgcc
     1921 gtcaaattga cgctgattac cgcgctgatt gccgttcccg tcaatgccgt cttgggcgtg
     1981 gcgatggcgt ggctgttgac ccgttttgac tttctcggca agcagttgct gaccaccctg
     2041 ctcgatttgc cgttttccgt atcgcccgtg gttgccggtt tgatgttcgt cttattgttc
     2101 ggcgcgcaca cggcgttggg cagccggctg gaagcgcaag gcatacagat tatcttcgcc
     2161 attcccggca ttgttttgac ggcgctgttc gttacctttc cctttgccgc acgcgaaatc
     2221 atccctttaa tgcagacaca gggcgacagc gaagaacagg cggcattggt actcggcgca
     2281 agcggttggc agatgttttg gcgcgttacc ctgcccaaca tcaaatggac gctgctctac
     2341 ggcatcatcc tcaccaacgc ccgtgcgatg ggcgagttcg gcgcggtcag cgtggtatcg
     2401 ggacacatac gcggcgaaac caacactatc ccgcttttgg tcgaaatctt ctacaacgaa
     2461 tacaacttca ccggcgcatt ctccctctcc ggcgtattgg cacttttagc actggcgacg
     2521 ctggcggtgc agaacatcat caccaaatta tagtggatta aatttaaatc aggacaaggc
     2581 gacgaagccg cagacagtac aaatagtacg gcaaggcgag gcaacgccgt actggtttaa
     2641 atttaatcca ctatacaaga caaaaaactc gccgccgccg aaaggaacgc agtatgagta
     2701 ttaccattca aaacttaaac aaaaaacgct tcggcaattt tcacgcgctg aaaaacatca
     2761 acctcaacgt ccccgccgac aaactcgttt ccctgctcgg tccctccggc ggcggcaaaa
     2821 ccacactttt acgcattatc gccggactgg aaaacgccga cggcggccaa attctctttg
     2881 acggccaaga cgtaaccgcc aagcatgtgc gcgagcgcaa agtcggtttc gtgttccaac
     2941 actacgccct cttccgccac atgaatgtgt ttgacaacgt cgctttcggt ttgaccgtat
     3001 tgcccaagcc cgaacgcccg tccaaaggac aaatccgcgc caaagtcgaa gaattgctca
     3061 aactcgtaca actctcccac ctcgccaaat cctatccgca ccaactctcc ggcgggcaac
     3121 gccagcgcat cgccctcgcc cgcgcgcttg cggtcgaacc gaaactcctg cttttggacg
     3181 aacccttcgg cgcgctggat gccaaagtgc gcaaagaatt acgcacctgg ctgcgcgaca
     3241 ttcatcacaa cctcggcgta accagcattc tggtgactca cgaccaagaa gaagccctcg
     3301 aagtttccga cgaaatcgtc gtgatgaacc atggcaaaat cgaacaaacc ggcagtgccg
     3361 aagccattta ccgcaaaccc gaaaacgcct tcgttaccga gttcctcggc gaagccgacg
     3421 catttgaagg acgcatcgaa aaaggcatct ggcattacaa cggcttcgcg tggaaattgg
     3481 acgcgcaata caaatggcag gagcaaaccg ccaccggcta tatccgcccg cacgaatggc
     3541 agatcgccgc cgaacacgaa accccgatga tccgtgccga aatcgaaaaa atccacgccg
     3601 tcggcgcatt gacgcacgtt ttggtgaaac acggcaaaca agacgtgcat atcacactgg
     3661 ctggcagcga tgccgcgcgc tacccaatcg ccgaaggcaa aaaattaaac ttaattccga
     3721 aacaggttta tgttttttct caaaacgagt tgattgaata ttcgatttaa ccctgaaagc
     3781 gcaatgccgt ctgaaaggct ttcagacggc attgcgtgtc aaacgtcagg caagaaacag
     3841 cttgtacgcg gcattttgcg tttcctcgtg atagctgtat cccagacttt ccaagaaacc
     3901 gtcaaatgcg gcggcatcgt gcggcggcac gtcgataccg accaaaatcc gcccgtaatc
     3961 cgcaccgtgg ttgcggtaat ggaagagcgt aatgttccaa ccgccctgca tatggttcaa
     4021 aaagcgcgcc aacgcgcccg gacgctccgg aaactcaaaa ctgaccagac gctcgtgccc
     4081 tactttgtcc gtccgccctc cgaccatata gcggatatgg attttggcaa tctcattatt
     4141 ggtcaaatcg acattgggca atcccgcctc atccaaccgg ctgccgataa ccgccaaatc
     4201 ctgcgggcct gccgcttgaa gtccgacaaa gatatgcgct ttttcatcgc cgccgtagcg
     4261 gtagttgaac tcggtaatat tcctatttcc caatatattg acaaacttaa ggaagctgcc
     4321 gcgttcttcg gggatggtaa cggcaaaaat accttcgttg ccctcgccca attcgctccg
     4381 ttccgaaacg tggcgcaaac ggtgaaaatt catattcgca ccgctggtaa cggcaatcag
     4441 ggtttggttt tccgcacctt ctcgggcaat ataggctttc agacccgcca acgccaacgc
     4501 gccggccggc tcggtaatgc tgcgcgtgtc atcgaaaata tccttgaccg cgccgcaaac
     4561 cgcatcggta tcgactgtaa tgatttcatc caaaagttct ttgcagaggc ggaaggttcc
     4621 gtttccgacg actttgaccg ccgtaccgtc tgaaaacagc ccgacatctt tcaaatggac
     4681 gatttcgcct gcttcgaccg actgcttcat acagcaggaa tcgttggtct gtacgccgat
     4741 aactttaatt tcgggacgaa cctgtttgat aaatgccgcc atgcccgccg ccaaaccgcc
     4801 gccccctatc ggtacgaata cggcgcggat tggatcggga tgctggctga caatttccat
     4861 ccccaccgtc ccctgtcccg caatcacatc gggatcgtca aacggcgcga tataggtcag
     4921 cccttcctgt tccgccagct ccatcgcata atcgtaggca tcgttgtatg aaacaccccg
     4981 caaaaccacc tcgccgccac ggcttttaac cgcatccact ttaattttcg gcgtagtctc
     5041 cggcatgacg ataacggcac ggcagcccaa acgctgtgcg gacaacgcca cgccttgtgc
     5101 gtgattgccc gcgcttgccg caatcacgcc gcaagcgagc gcatctttcg gcaacttgga
     5161 cattttgttg tacgcgccgc gtattttgaa cgaaaaaacc ggctgcaaat cttcgcgttt
     5221 caaaaggatg ttgtttttca aacgtacaga aaggctgcgt gccggttcca aaggcgtttc
     5281 gaccgctaca tcatagacag atgccgtcag gatgcggatg aggtaatcgg aataaggaag
     5341 gggcgtgttc ataattcaat gcgggataat cggtttatta aaatcgcaaa acccaaaacc
     5401 atacgcccaa gacggcgcga aagcaagaaa aatccgcccg atcagacacc ctaagcgtat
     5461 aatcggcagg ctgaaacact cacacaattt agaatatttc atgacagcac ataaaatcct
     5521 gcccgtcctt cttcccatca tcttaggcgt ttctcacgca acggctgcat cgcccgcgcc
     5581 caacagaccg acggtacacg ccgcccccac gctccaaaca cccgaaaccc tcacggcggc
     5641 acacatcgtt atcgaccttc aaagcaggca gactttatcc gccaaaaaca ccaatacccc
     5701 tgtcgaaccg gcggcactaa cccaactgat gaccgcatat ttggttttca aaaacatgaa
     5761 atcgggaaat atccaatctg aagaaaactt aaaaataccc gaatccgcat gggcttcaga
     5821 aggaagcaga atgtttgtac gtcccggcga tacggtcagc accgacaaac tcttaaaagg
     5881 catgattgcc ctatgcgcaa acgatgccgc cctaaccctt gccgaccggc tgggcaacgg
     5941 ctcgattgaa aattttgtgc aacaaatgaa caaagaagcc cgacgcttgg gcatgaagaa
     6001 caccgtattc aaaaacccga caggcttggg tagagaagga caggtttcca ccgccaaaga
     6061 cctctccctg ctgtctgaag cattgatgcg cgactttccg gaatattacc cgctgttttc
     6121 catcaaatcg ttcaagtttg aaaacataga acaaaacaac cgcaatatcc ttttatatag
     6181 ggacaacaat gtaaacggcc tgaaagccgg gcacacagaa agcggcggct acaaccttgc
     6241 cgtgtcatac tccggcaacg gcaggcacat ccttgtcatc acactaggtt cggaatcggc
     6301 ggaaacccgc gcatcggaca acagcaagct gctgaaccgg gcattgcagg ccttcgatac
     6361 gcccaaaata tatccgaaag gcaaaaccgt tgcccaaatc caaatttccg gaggcagcaa
     6421 aaaaaccgtc cgcgcaggct tcctcaaaga agcctacatc actctgccac ataaagaagc
     6481 gaaaatggca gaacagattt tggaaaccat acagccgatt cccgccccgg taaaaaaagg
     6541 gcagatttta ggaaaaatca aaatcaggca aaacggacat accattgccg aaaaagaaat
     6601 cgtcgcactg gaaaacgtag aaaaaagaag ccggtggcaa aggctttgga cgcgtctgac
     6661 agggcagtaa tctgccgttt caaatatccc gcttttccaa caaataaaga aatgccgtct
     6721 gaaacacggt tcagacggca taaaacaaca gggcggtacg tattgcatac gcgccgccct
     6781 gctgatggaa gtaagttagc gctttttacc ggtaacggta gcaacagcca gattttcgtt
     6841 acgtttcagg gaaacgcttt ctacaccttc aggcagtttg atgtctgaca agtgcaaaat
     6901 gtcgccggca accacttcgg cacaatccaa atccaagaaa gcagggatat tggcaggcaa
     6961 cgcaagcact tcaacagccg tgttcagcag agatacgcga ccgccttgca atttaacggc
     7021 ttgagaattt tcagcgttaa caatgtgcag gggaacgcgg atgcgtacaa gttgatcggc
     7081 tttcacagct tggaagtcga tgtgttggac ttcgcggcgg aacgggtgca tttggaaatc
     7141 gcggacgata acgtctttgg tttcgccgtt cagagacaat ttaatcaacg cagtatggaa
     7201 agattctttt tccaatgcgt aaaatacggt tttgtgatct acggcaatgg caacaggctc
     7261 ttgaccttca ccgtacagaa taccggggat ttggccttcg cgacgcaggc ggcggctcgc
     7321 accagtgcct tgtgcttcac gaacagaggc ttgaatttca taagtcatgt taaatactcc
     7381 aagttaggta aaatcgccgt catcggccgc gaccagctta agacggcatc ggggttacgg
     7441 cagcaacatg ctgcctgtca tcacttcttc attgaaaaga tatgagacgg attcttcatt
     7501 gctaatgcgg cggacggttt cggccaacaa acctgcaatc gttacctgac ggatacggtc
     7561 gcagttttta gccgcttcag acaaaggaat ggtatcggtt acgaccacct ggtcgatttc
     7621 ggatgaggca atacggctga ccgcctctcc ggagaatacg gcgtggctgg cataggctag
     7681 aacaccttcc gccccccgct ctttcaaggc gacggcggct ttgcacagcg tatttgcagt
     7741 gtcaatcata tcgtccacaa tcagacaggt tttgccttga atatcgccga tgatgttcat
     7801 aacttccgcc acattggctt tcgggcggcg tttgtcgatg attgccaagt cggcattcaa
     7861 agattttgcc acggcgcggg cgcggacgac accgccgatg tccgggctga cgacggtcag
     7921 attttcaatc cgctgctgct tgatgtcgtt caacaaaatc ggagtggcat aaatattgtc
     7981 caccggaata tcgaagaaac cttgaatctg gtcggcatgc aaatcgacag tcaaaacacg
     8041 gtcgatccct gccgaataca gcatatttgc caccagtttg gcagaaatcg gaacgcggac
     8101 ggaacgcgga cggcggtctt ggcgcgcata gccgaaatac ggaatggctg tggtaatacg
     8161 acctgccgaa gcacgcttca gtgcatccgc catcgtcagg atttccatca ggttgtcatt
     8221 ggtcggcgca caggtcggct gaaggatgaa aacgtcgcgc ccgcgtacgt tttccaacag
     8281 ttcgacggca acttcgccgt ctgaaaactt ggatacggaa gcattgccca aagaaatgtc
     8341 caaatgcctg acaacacgtt gtgccaattc gggattggca ttgcctgtaa ataccatcaa
     8401 actgtcgtac gcagccatat tctcacctga ttttatgttt aacttccgct cagaaaacac
     8461 aatgcttccc tccgcttgta ccaagcccgg cattatacct gcaacatccg acacaaacaa
     8521 aggcgtttca atatttttat ttctatgaaa taaaaagcgt gtaagcaggc ttacacgctt
     8581 ttgtctggct ggggaggaag gattcgaacc ttcgcatgct ggaatcaaaa tccagtgtct
     8641 taaccgcttg acgactcccc aacttgtttg gcttggctgg ggaggaagga ttcgaacctt
     8701 cgcatgctgg aatcaaaatc cagtgtctta accgcttgac gactccccaa caatgtttat
     8761 acggacaaca aaggatgttt tgaaagaccc tctgccaaat atgcctcgta caaacctgaa
     8821 acttgtcggt atatattgta tgcgctattc ctagcttgaa atgctgcaaa tatacaggct
     8881 cctgagccgg tcatcattgc agaaccgtat ttcgacaact cggaataagc tttccaaact
     8941 tcagggtatt ctttaaatac cactgcctgc atatcgtttc tgaacggttg caggttttgg
     9001 aaagtcggca ttatgcttga ggcagaatcc cgtgtcaagc cttcgtatgt gaaaattttt
     9061 gcggtggaaa cgtggacggg aggtttgaca ataacatacc actgtttcgg aatatccatt
     9121 cctatcagtt tttcacctat cccgcttgca aaagcatttt ttccgaaaat aaaaaacggt
     9181 acgtccgcac ccaaagccgc acccaaatca atgagccgcc actgcgtcaa accacactgc
     9241 caccaacggt tcaacaccag caaaaccgtt gcggcatcgg aactcccccc tcccaagccg
     9301 gcgccggtcg ggattttttt gtccagccat atttccacgc cggcaaggtt gcgcgcatat
     9361 ttttgcagca acgatgcggc acggtagctc aaatcggctt cctgcggtat tccgccgaca
     9421 ggattgtgca ggatgatttt gccgtcgtcc ctcggtttca aatatacggt atcctgcaaa
     9481 tctatcaggc agaatatgct ttcgatattg tgataaccgt cttcccgcct gccggtaatc
     9541 ctcaaatcga gattcagttt tgcaggtgcg ggaaacgcct gccgtccgtc cgcaatattc
     9601 atctgtccgc cttatctcgt gcgcgccgca cagcgttccg gggtttcggt ttcagacggc
     9661 attccaattt cggtgaaaac cagcctgatg ttcaaatttc cgttattcag ttgcaacgtt
     9721 cggacttgcc ccccactgtc ggcggttctg ccgattgtcc aaccgtattg ttccaatatg
     9781 ccgtctgaac ggatgcggta aggcgcgccc gccacacgcc tgccttccgc ccagatatgc
     9841 agatattgga ttggcagttt gaaaccgacc agctgcctgc tcaagtcttc cgtaccctct
     9901 gcctgataga catttccttt gccgtccact gccaatgcgc cgtccctgtc ttgacacaac
     9961 tgtccgagcg tgccgcccaa aggggtgttg atattgatgg tttccacggg cggttggtat
    10021 gtccaatcga aatttgcata cgaacctttc ccttccgctt tgactgccaa ccgcccttcc
    10081 gctgcaaaac tgctgatgtg ttcggacggc tgccacaggt tttcgttatt ttgaggtaat
    10141 tgcgcgcaag cggtcaaaag caggatgacc gatgcggata cggtgtgttt catcagagtt
    10201 tccttaacgg gtgtcatcct gccggccgac gggtgcggcg tgccgtgcaa aacgcgtgcg
    10261 aactgcgtgc cgtttgcccg aatgccccgg cttcccggca aaccttatgc cgtctgaaag
    10321 gatgggcctg cattatttcc ggggttttcg ggaaggctcg ggcaaggcga ttccgtagcg
    10381 tttgagcgtc tcccgccata ttttcttgtc tcccgtaagg tgtgccgcct gcgtccatac
    10441 gtcaaccgcc tgatcgcgtt cgcccaatgc ccacaacact tcgcccaaat gggcggcaac
    10501 ttcgggctcg gggtcgtttt caaacgaata ccgcagatac ggcagcgcgc tttccgcgtc
    10561 gcctttcagg taatacgccc agcctatgct gtcgttaacg gcggtatcgt ccgggttgat
    10621 ttggtatgcc gtctgaagca gggcgaaacc ctcgtccaaa cgtttggaat cggaaagcag
    10681 gctgtagccc agattattca taatttgtgc attatcgggc gtaagtttga gcgcggtttc
    10741 aaggtcggca atcatttttc cccgtttgcc gaactgttcg taaataatgg aacgctgtgc
    10801 caatgcttcc gccaaaggtt ccgtgcttcc cgccgccgaa agtttggcga tgatgttgtt
    10861 cagcccgatc agggcttccc gtttgtcggg cagcttcgac agggcgagca tctgtatttt
    10921 ggacaaattg tctgccgtaa aataccgccc ctgctgttcg ggaagtttcc gcaccctgcc
    10981 gatctgccgc aaagccgccc ggcctccgtc caattcggca gccgccgcag ccgccagcac
    11041 gcctttgtcg aacaggtatt ccggcgcgga cacttttttc aaccactgcc tgactttggc
    11101 gtaatccctg cggtcggcat atatcatcgc cgccgtcatt gccgccctgc cccgctgttc
    11161 ccccgtcccc ctgccgtatg ccttttcggc gtagccgtcg ataacggacg caccttcttt
    11221 tcggtttgcc gccaatatcg ccgcctgaat atacaggttt gcattcgggt tgtgttccaa
    11281 cagcacgttc aaacgcgcat aggcatcatc cggcttacgc agggaaacca gattcataat
    11341 ttccatttcc tgccagacgg ccgaaaggtt ttgggtgtct gtctgctcga aaaagccgtc
    11401 gagtatttcg ggatatttgc gtgcagtcag acgcaacgtc attaaagtgg ggggcaatat
    11461 ttccgtatcg agcttcgcca aacgctgcaa agcttcgatt gccttttcct tttcgcgtcc
    11521 ctgtacgccg aacaccgcat cggcaaccgc cgcttcgggc agatgttcat acttcaacgc
    11581 cgcacggcga accgctttcg atgctttttg agccacccca ccctgctgca cggcggcttg
    11641 caccagcagc aaaaatatcc tgcgtttttg cacatcgtcc gattgcgcca gcacctcttc
    11701 caacccgtcc agatgctgat ttcccccttc cctcaatacg ttccgcagcc accccgcccg
    11761 tttttgcgcc tcacccggta taggctcgat ctgccgccat ttctgataaa tcatttccgc
    11821 ctgttcaaac gcgttcagcg acacggccat ttccaaggcg cgttcggcga cttcggggga
    11881 ttttgtgcgt tccaacatca gcatataggt tgccagagcc gttcccgcct gccctttctg
    11941 caaagccgtt tcaccgccca acagcgtaaa caccctgttg acccgttcgc ccactgccgc
    12001 aagccgtgcg cgttcgtttt tgatttcttc ctcgctgtaa cgccgatgtt tccttaaaac
    12061 ctttccgact tccttcggca gctccacatc cgccgcgccg gcagcatacg cctgtccggc
    12121 aagcagggct gctgcgagga cagataaaat agtgaaacgg gcgggtaaca taatcatcct
    12181 ttatccgaca tccgtccgaa ccttcagacg gcatattgag tatctagaca aggcgtcact
    12241 ttatcacaag ccaaacggtt tgtagatttg caaaacccgc ccgaaggcag ggctgtgttt
    12301 tgagattgcg tcggtttgcc tgtatgcgat gccggacaat ccttgcctta ttatttggtt
    12361 tgacagttta atttgttgaa caaaaagaaa tttatttcat tccgtctatt ttccgacatt
    12421 attccgaagc acagccgacg gcaaaatgcc gtctgaaacg caaatccgct tcaggcggca
    12481 ttcccggctc cggcgggcta aggcaggccg acttccacat tgtcaatcag acgcgtcgtc
    12541 cccagacggg cggcggccaa gaccaccagt ttcttatctc ccgcccgcgc catttcgagc
    12601 gtatcggcgc ggcggatttc cacataatcg accagccagc ccgcagcggt caaatggtcg
    12661 gcagcctgcc gctccaaacc cgcataatcc aaaccgccct gcttcagcga ttcggcaaca
    12721 gcctgcagct cgcggtacag gcgcggtgct tcggcgcgtt ccccaacgct caagtaccgg
    12781 ttgcggctgg agagtgccaa accgtccgcc gcccgccccg tatcgacggg gacaatttcg
    12841 atatcgaaat tcaaatcctc ggtcaaacct ttaatcacgg caagctgctg gtaatctttt
    12901 ttgccgaaac aggcaacatc cggcaaaacg atattgaaca gcttggacac caccgtcgcc
    12961 acgccgcgaa aatggccggg gcggaatttg ccgcacaatt cgttttgcaa atggggcggt
    13021 tcgacgttgt aacgctgttc cacgttcgga tagagttctt tctcatcggg cgcgaaaaca
    13081 acggcaacgc cttcggcggc aagtttgtcc gcatcctgtt gcaaagtacg cggatatttg
    13141 tcgaaatcct cgccctgacc gaattgcaag cggttgacga atatgctgac aacaacgttg
    13201 tcggcgcgtt ttctggcttc gcgaacaagg gcaagatgcc cttcatgcag attgcccatg
    13261 gtcggcacaa atgccacctt tcctatattt tcacgccacg tgcgcagttc tcgaatggta
    13321 tgtatgattt gcattacggt aatccttatc cttcgggctt cctgcccgac aaaacgcgcc
    13381 gattatacgc gccgcgcacg gcaaaacaaa atgccgtctg aaacggcttt cagacggcat
    13441 cggcagcaga ccttgttcaa tctgcaaaaa tatgttccgc agcagggaag gttttggctt
    13501 tgacttcggc aacatacgcc cgaaccgcag cctggatgct gctttgcccg cgcataaagt
    13561 ttttgacgaa tttcgccgtt ttgcccggga aaataccgag catatcgtgc atcaccaaaa
    13621 cctgaccgtc gcaatccgcg cccgccccga tgccgatggt cggacaggaa acagtttcag
    13681 ttaccttttt tgccagttcc gccggcacgc attccatcag tacgacggca gctccggctt
    13741 catcatgcac cttggcatcg ttaagcaacg cctgcgcctt gccgccgcgc ccctgaactt
    13801 tatatccgcc gaaggcaaac acggattgcg gggtcagacc gatgtgggca cagaccggaa
    13861 ttccgcgcat ttgcagaaat tcagtcgttt ccgccatcca cacgccgcct tcgagtttga
    13921 ccatatgcgc gccggcagcc atcagttcgg cggcggcggc aaacgcctgc tccttactct
    13981 gctgatatgc accaaacggc aaatcgctga caatcatcgc attttttgca ccgcgtgcta
    14041 cacattcggt gtgatagcac atatcgcgca ggctgaccgg cagcgtcgac tgtcgcccct
    14101 gaaccgccat tcccaaagaa tccccgacca acagcacatc cacgccggca tcgtccatca
    14161 gcgcggcaaa actggattcg taagcggtca gcataacgat tttctcgccg gccgccttca
    14221 ttttttgcag tgtgttcaca gtaatcatca gatattgtta ccccgccctt ttcagacggc
    14281 ct
//
LOCUS       DAXFZR010000057        14246 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000057 DAXFZR010000000
VERSION     DAXFZR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14246
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.057"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            317..1192
                     /gene="prpB"
                     /locus_tag="ACFJAS_001501"
     CDS             317..1192
                     /gene="prpB"
                     /locus_tag="ACFJAS_001501"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC3982715.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNAYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            1277..2425
                     /gene="prpC"
                     /locus_tag="ACFJAS_001502"
     CDS             1277..2425
                     /gene="prpC"
                     /locus_tag="ACFJAS_001502"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC3982716.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLETLPAHTHP
                     MDVMRTGVPMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETVGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAECLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYAGPEDLAFVEIEER"
     gene            2577..3365
                     /locus_tag="ACFJAS_001503"
     CDS             2577..3365
                     /locus_tag="ACFJAS_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC3982717.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFIALYFVFSPKLDGSKEGKARMSFFLFGLTVALLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            3442..6047
                     /gene="acnD"
                     /locus_tag="ACFJAS_001504"
                     /pseudo
     CDS             3442..6047
                     /gene="acnD"
                     /locus_tag="ACFJAS_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            6597..7759
                     /gene="prpF"
                     /locus_tag="ACFJAS_001505"
                     /pseudo
     CDS             6597..7759
                     /gene="prpF"
                     /locus_tag="ACFJAS_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            8071..9270
                     /locus_tag="ACFJAS_001506"
     CDS             8071..9270
                     /locus_tag="ACFJAS_001506"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC3982718.1"
                     /translation="MSDQLILVLNCVSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGRRIAHGGEKYHESVL
                     IDQDVLDELKACIPFAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNEKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTYRLAKYIASMAVACGSVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            9381..10001
                     /locus_tag="ACFJAS_001507"
     CDS             9381..10001
                     /locus_tag="ACFJAS_001507"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC3982719.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(10025..10153)
                     /locus_tag="ACFJAS_001508"
     CDS             complement(10025..10153)
                     /locus_tag="ACFJAS_001508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982720.1"
                     /translation="MPPKPAFGRHFVCRFKRIFRPGAIRAGISGCRRQGARCRLNG"
     gene            complement(10163..10606)
                     /locus_tag="ACFJAS_001509"
     CDS             complement(10163..10606)
                     /locus_tag="ACFJAS_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC3982721.1"
                     /translation="MYLTQHTDYGLRVLIYTAVNDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            10780..11148
                     /locus_tag="ACFJAS_001510"
     CDS             10780..11148
                     /locus_tag="ACFJAS_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC3982722.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            11193..12347
                     /locus_tag="ACFJAS_001511"
     CDS             11193..12347
                     /locus_tag="ACFJAS_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC3982723.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(12405..13277)
                     /locus_tag="ACFJAS_001512"
     CDS             complement(12405..13277)
                     /locus_tag="ACFJAS_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC3982724.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            13362..14174
                     /locus_tag="ACFJAS_001513"
     CDS             13362..14174
                     /locus_tag="ACFJAS_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC3982725.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVLDAV"
BASE COUNT         3220 a         3886 c         3984 g         3156 t
ORIGIN      
        1 cagtcttgtg ttttagatta ccgaaaaata aaactacata atactacaaa ggaacattac
       61 tatgaaacca attcagatgt ttttcccttt tctgaataat ccccttgttt tcttcttgtc
      121 tgcggttttg ccgcataatt ccgaacggtc tgctgttttt ctttgattcg ttttaaatat
      181 caataagata atttttccca tatattttta atgattggat tgggatgccc gacgcgtcgg
      241 atggctgtgt tttgccgtcc gaatgtgatg gaagcctgtc catactgaaa aaaaaagtct
      301 ataaaggaga aatatgatga gtcaacactc tgccggagca cgtttccgcc aagctgtgaa
      361 agaatcgaat ccgcttgccg tcgccggttg cgtcaatgct tattttgcac gattggccac
      421 ccaaagcggt ttcaaagcca tctatctgtc cggcggcggc gtggcagcct gttcttgcgg
      481 tatccctgat ttgggcatta ccacaatgga agatgtgcta attgacgcac gacgcattac
      541 ggacaacgtg gatacgcctc tgctggtgga catcgatgtg ggttggggcg gtgcattcaa
      601 tattgcccgt accattcgca actttgaacg cgccggtgtt gcagcggttc acatcgaaga
      661 tcaggtagcg caaaaacgct gcggccaccg tccgaacaaa gccatcgttt ccaaagatga
      721 aatggtcgac cgcatcaaag ctgccgtaga tgcgcgcgtt gatgagaact tcgtgattat
      781 ggcgcgtacc gatgcgctgg cggtagaagg tttggatgcc gctatcgaac gcgctcaggc
      841 ttgtgtcgaa gccggtgcgg acatgatttt ccctgaagcc atgaccgatt tgaaaatgta
      901 ccgtcaattt gcagatgcag tgaaagtacc tgtattggcc aacattaccg agtttggtgc
      961 tacaccgctt tatacgcaaa gcgagttggc tgaaaacggc gtgtcactgg tgctgtatcc
     1021 gttgtcatca ttccgcgcag caagcaaagc cgctctgaat gcttacgaag cgattatgcg
     1081 cgatggcact caggcggcgg tggtggacag tatgcaaacc cgtgccgagc tgtacgagca
     1141 tctgaactat catgccttcg atcaaaaact ggataaattg tttcaaaaat gatttaccgc
     1201 tttcagacgg cctttcaaca aatccgcatc gaccgtctga aaaataaaac ccataaaaat
     1261 acaaaggaga aataccatga ctgaaactac tcaaaccccg accttcaaac ctaaaaaatc
     1321 cgttgcgctt tcaggtgttg cggccggtaa taccgctttg tgtaccgttg gccgtaccgg
     1381 taacgatttg agccatcgcg gttacgacat tctaaatttg gcacaaaaat gcgagtttga
     1441 agaagtcgca cacttgctga ttcacggcca tctgcccaac aaattcgagc tggccgctta
     1501 taaaaccaag ctcaaatcca tgcgcggcct gcctatccgt gtgattaaag ttttggaaac
     1561 cctgcctgca catacccatc cgatggacgt gatgcgtacc ggcgtaccca tgctgggctg
     1621 cgttcatccc gaacgcgaaa gccatcccga cagcgaagcg cgcgacatcg ccgacaaact
     1681 gatcgccagc ctcggcagca tcctgctcta ctggtatcaa tattcgcaca acggcaaacg
     1741 tattgaagtt gaaagcgacg aagagaccgt cggcggtcat ttcctgcacc tgttgcacgg
     1801 caaacgccca agcgaatcac acatcaaagc catgcatgtt tcactgattc tgtatgccga
     1861 acacgagttc aacgcctcca cttttactgc ccgcgtgatt gccggtacgg gttcggatat
     1921 gtattcctgc atcaccggcg ccatcggcgc gcttaaaggt ccgaaacacg gcggtgcgaa
     1981 cgaagtcgcc tacgacatcc aaaaacgcta ccgcaacgcc gacgaagccg acatccgcga
     2041 acgcatcggc cgcaaagaaa tcgtgatcgg tttcggccat ccggtttaca ccatttccga
     2101 cccgcgcaac gtcgtgatta aagaagtggc gcgcggtttg agccgggaag cgggcgacat
     2161 gcgcctcttc gacatcgccg aatgcttgga gagcgtgatg tgggaagaga aaaaaatgtt
     2221 cccgaatctg gactggttct ctgccgtttc ctaccaaaaa ttgggcgtac cgaccgctat
     2281 gttcacaccg ctgttcgtga tttcccgtac aaccggttgg agcgcacacg tcctcgaaca
     2341 gcgcaaagac ggcaaaatca tccgtccgag cgcaaactac gccggccctg aagatttggc
     2401 gtttgtggag attgaagaac gataattgaa gaatgcaata gcagtttgtt ctttatttcg
     2461 gtatgcaaag ctgaggattt cagacgacct tgccttattg gaaaggttgt ctgaaataag
     2521 tttaatctaa taggagaaga taatcctgta ttggcgcaag taacaggata agaaacatgg
     2581 aagatttata cataatactc gctttgggtt tggttgcgat gatcgccgga tttatcgatg
     2641 cgattgcggg cgggggtggt ttgattacgc tgcctgcact cttgttggca ggtattcctc
     2701 ccgtgtcggc aattgccacc aacaagctgc aagcagccgc tgctacgttt tcggctacgg
     2761 tttcttttgc acgcaaaggt ttgattgatt ggaagaaagg tctcccgatt gccgcagcat
     2821 cgtttgcagg cggcgtggtc ggtgcattat cggtcagctt ggtttccaaa gatattttgc
     2881 tggcggtcgt gccggttttg ttgatattta tcgcgctgta ttttgtgttt tcgcccaagc
     2941 tcgacggcag taaggaaggc aaagccagaa tgtctttttt tctattcggg ctgacggttg
     3001 cactgctttt gggtttttac gacggtgtgt tcggaccggg tgtcggctcg ttttttctga
     3061 ttgcctttat tgttttgctc ggctgcaagc tgttgaacgc gatgtcttac accaaattgg
     3121 cgaacgttgc ttgcaatctt ggttcgctat cggtattcct gctgcacggt tcgattattt
     3181 tcccgattgt ggcaacgatg gcggtcggtg cgtttgtcgg tgcgaattta ggtgcgagat
     3241 ttgccgtccg cttcggttcg aagctgatta agccgctgct gattgtcatc agcatttcga
     3301 tggctgtgaa attgttgata gacgagagaa atccgctgta tcagatgatt gtttcgatgt
     3361 tttaaaccct ttcagacgac tccttcaaaa catcggctga aacccaaacc acaagaaaaa
     3421 tagatccaca ggagaaccga catgactgcc aaccaaagtt accgcaaacc gctgcccggt
     3481 acggatttgg aatactacga cgcgcgtgcg gcgtgtgagg acatcaaacc cggctcttac
     3541 gacaagctgc cgtacacgag ccgcattttg tctgagaact tggtcaaccg cgcggacaaa
     3601 gtcgatttgc cgatgctgca aagctggctg ggccagttga tagaagggaa gcaggaaatc
     3661 gattttccgt ggtatcccgc gcgggtggtg tgccacgata tcctggggca gaccgcgctg
     3721 gtggatttgg caggcttgcg cgatgcgatt gccgaaaaag gcggcgatcc tgccaaagtg
     3781 aatccggtgg tgcagacgca gcttatcgtc gaccactctt tggcggtgga gtgcggcggt
     3841 tacgatcctg atgccttccg caaaaaccgc gaaatcgaag acagacgtaa cgaagaccgt
     3901 ttccacttca tcaactggac aaaaaccgca tttgaaaatg tggacgtgat tccggcgggc
     3961 aacggcatca tgcaccaaat caatctggaa aaaatgtcgc ccgtcgtcca agtcaaaaac
     4021 ggcgtggctt tccccgatac ctgcgtcggc acggactcgc atacgccgca cgttgatgcg
     4081 ctgggcgtga tttccgtggc gtgggcggtt tggaagcgga aaccgtgatg ctgggacgcg
     4141 cgtccatgat gcgcctgccc gatattgtcg gcgtggagct gacgggcaaa cggcaggcgg
     4201 gcattactgc cacagacatc gtgttggcac tgaccgaatt cttgcgtaaa gagcgcgtgg
     4261 tcggggcgtt tgtcgaattt ttcggcgagg gcgcgagaag cctgtctatc ggcgaccgcg
     4321 cgaccatttc caacatgacg ccggagttcg gcgcgactgc cgccatgttc gccatcgacg
     4381 cgcaaactat tgattatttg aaactgaccg gacgtgacga cgcgcaggtg aaattggtgg
     4441 aaacctacgc caaaaccgca ggcttatggg caggtggctt gaaaaccgcc gtttatccgc
     4501 gcgttttgaa atttgatttg agcagcgtaa cgcgcaatat ggcaggcccg agcaacccgc
     4561 acgcgcgttt tgccaccgcc gatttggcgg cgaaagggct ggcgaagcct tacgaagagc
     4621 cttcagacgg ccaaatgcct gacggtgcag tgattattgc cgcgattact tcgtgtacca
     4681 atacttccaa cccgcgcaac gttgtcgccg ccgcactgtt ggcacgcaat gccaaccgcc
     4741 tcggcttgaa acgcaaacct tgggtgaaat cttcgtttgc cccgggttca aaagtagccg
     4801 gaatctattt gaaagaagca ggcttgttgc ccgaaatgga aaaactcggc ttcggtatcg
     4861 tcgccttcgc atgtaccacc tgtaacggca tgagcggcgc gctcgacccg aaaatccaac
     4921 aagaaatcat cgaccgcgat ttgtacgcca ccgccgtatt gtcaggcaac cgcaacttcg
     4981 acggccgtat ccatccgtat gcgaaacagg ctttcctcgc ttcgcctcct ttggtcgttg
     5041 cctacgcatt ggcaggtagc atccgtttcg atattgaaaa cgacgtactc ggcgttgcag
     5101 acggccgcga aatccgcctg aaagatatct ggccgacaga cgaagaaatc gatgccatcg
     5161 ttgccgaata tgtgaaaccg caacaattcc gcgacattta tatcccgatg tccgacaccg
     5221 gcacagcgca aaaagcacca agcccgctgt acgactggcg accgatgtcc acctacatcc
     5281 gccgtccgcc ctattgggaa ggcgcactgg caggggaacg tacattaaga ggtatgcgtc
     5341 cgccggcgat tttgcccgac aacatcacca ccgaccacat ctcgccatcc aatgcgattt
     5401 tggccggcag tgccgcaggt gaatatttgg cgaaaatggg tttgcctgaa gaagacttca
     5461 actcttacgc aacccaccgc ggcgaccact tgaccgccca acgcgcaacc ttcgccaatc
     5521 cgaaactgtt taacgaaatg gtgagaaacg aagacggcag cgtacgccaa ggttcgttgg
     5581 cacgcgttga accagaaggc caaaccatgc gcatgtggga agccatcgaa acctatatga
     5641 accgcaaaca gccgcttatc atcattgccg gtgcggacta tggtcaaggc tcaagccgcg
     5701 accgggcggc gaagggcgtg cggctggcgg gtgtggaagc catcgccgcc gaaggtttcg
     5761 agcgcatcca ccgcaccaac ctcatcggca tgggcgtctt gccgctgcaa ttcaaacccg
     5821 gcaccaaccg ccataccctg caactggacg gtacggaaac ctacgacgtt gtcggcgaac
     5881 gcacaccgcg ctgcggcctg accctcgtga ttcaccgtaa aaacggagaa accgtcgaag
     5941 ttccggttac ctgccgcccc gataccgcag aagaagtatt ggtatatgaa gccggcggcg
     6001 tattgcaacg gtttgcacag gactttttgg aagggaacgc ggcttagagt tcgtctgaaa
     6061 agcaagacgt agcgtgggtc gggttctcat tcacgtaatt cccgatatgg caggcattta
     6121 ctgtaaatcg tcattcccgc gcaggcggga atccagaaag tggaattgag gaaacctttt
     6181 tatccgatga gtttctgtgc ggataaatct ggatttccgc ctacgcggga atgacggggt
     6241 ttaataatct accgtatcac aacacagcag ccgtagattg gggcgaaccc cgacagtttg
     6301 cggaatcaaa cggctttggt cggagtggta gcctaatgta cttctggaaa gtgggtgtag
     6361 cgtgggcttt gcccacgaaa taaaggctga attgacatgg tatagcggat taacaaaaat
     6421 caggacaagg cggcgggcct caggcagtac ggatagtacg gaaccggttc gcccggtgct
     6481 tgggcgcctt agggaaccgt tccctttgag ccggggcggg gcaacccgta ccggtttaaa
     6541 ttaatccact ataaaaatcg tgggcagagc ccacgctaca taaggagaac cctaaaatgc
     6601 cgcaaatcaa aatccccgcc gtttactacc gcggcggtac atctaaaggc attttcttca
     6661 aacgcaccga tctgccgaca gctgcgcagg aagcaggcgg ggcacgcgac aaaatcctct
     6721 tgcgcgtact cggcagcccc gacccttacg gcaaacagat tgacggtttg ggcaacgcca
     6781 gttcgtccac cagcaaagct gtgatttcgg acaaatccga acgcgccgat cacgatgtcg
     6841 attacttgtt cggacaagtt tccatcgaca aaccatttgt cgattggagc ggcaactgcg
     6901 gcaaccttac cgccgccgtg ggcgcattcg ccatcgaaca gggcttggtc gataaatcca
     6961 aaatcccttc agacggcatc tgcacggtca aaatctggca gaaaaacatc ggcaaaacca
     7021 ttattgccca tgtgccgatg caaaacggag cagtgttgga aacaggcgat ttcgagcttg
     7081 acggcgtaat tttccggcag ccgaagtaca aatcgaattt ttagatccag ccgacggcga
     7141 aggcagtatg ttcccaaccg gcaatttggt cgacgaaatt gatgtgccga atataggtcg
     7201 tctgaaagcc acgctcatca acgcgggcat tccgaccgtt ttcctgaacg ccgccgactt
     7261 gggctacacg ggcaaagagt tgcaggacga catcaacaac gatgccgccg cgctggaaaa
     7321 atttgaaacc atccgcgcat atggcgcgct gaaaatgggt ttgatcagcg acgtatccga
     7381 agccgccgcc cgcgcgcaca cgccgaaacc cgccttcgtc gcgcccgccg ccgattacac
     7441 cgcctccagc ggcaaaaccg taaacgccgc cgacatcgat ttgccggtac gcgccctgag
     7501 catgggcaaa ctgcaccacg ctatgatggg catcgcctcg gtcgccatcg ccgccgccgt
     7561 gctcggtacg ctggtcaacc ttgccgcagg cggcggaacg cgtaaagaag tgcgcttcgg
     7621 gcatccgtca ggtacgctgc gtgtcggtgc tgccgccgaa tgtcaggacg gacaatggac
     7681 ggccgccaaa gcggtcatga gccgcagcgc acgcgtgatt atggaaggtt gggtgcgcgt
     7741 tcccgatgat tgtttttgaa atcatgtagg acgcacattg ctgtccgctg tttcagacgg
     7801 catttgatta aggattacca taaagccgaa taacctcacc gccgtcattc ccacgcaggc
     7861 gggaatccgc agccgatatt ctgtttcctg atttttatca gagcctgcgt gtgagcaaat
     7921 atggattccc gcctgcgtgg gaatgacgct aggaaatacg gcatacgctt tgcccaaaga
     7981 ggccgtctga aacacactcc gcccaacgcc catccttttt cagacggccc cgcaccaaaa
     8041 aaacaaccac aaactacaag gagaaacatc atgtccgacc aactcattct tgtcctgaac
     8101 tgcgtcagtt catcgctcaa aggcgccgtt atcgaccgca aaagcggcag cgtcgtccta
     8161 agctgcctcg gggaacgcct gactacgccc gaagccgtca ttaccttcaa caaagacggc
     8221 aacaaacgcc aagttcccct gagcggccgc aactgccacg ccggcgcggt gggtatgctg
     8281 ttgaacgaac tggaaaaaca cggactgcac gaccgcatca aagccatcgg ccgccgcatc
     8341 gcccacggcg gcgaaaaata tcacgagtcc gtcctcatcg accaagacgt ccttgacgaa
     8401 ctgaaagcct gcatcccgtt cgccccgctg cacaaccccg ccaacatcag cggcatcctc
     8461 gccgcgcagg aacactttcc cggcctgccc aacgtcggcg tgatggacac ctcgttccac
     8521 caaaccatgc cggagcgggc ctacacttat gccgtgccgc gcgaattgcg caaaaaatac
     8581 gccttccgcc gctacggttt ccacggtacc ggtatgcgtt acgtcgcccc tgaagccgca
     8641 cgcatcttgg gcaaacctct ggaagacatc cgcatgatta ttgcccactt aggcaacggc
     8701 gcatccatta ccgccgtcaa aaacggcaaa tccgtcgata ccggtatggg tttcacgccg
     8761 atcgaaggtt tggtaatggg tacacgttgc ggcgacaccg atccgggcgt atacagctat
     8821 ccgactttcc acgcagggat ggatgttgcc caagttgatg aaatgctgaa cgaaaaatca
     8881 ggtttccccg gtatttccga acttcccaac gactgccgca ccctcgaaat cgccgccgac
     8941 gaaggccgcg aaggcgcgcg cctcgccctc gaagtcatga cctaccgcct cgccaaatac
     9001 atcgcttcga tggctgtggc ctgcggcagt gttgacgcac tcgtgttcac cggcggtatc
     9061 ggcgaaaact cgcgtaatat ccgtgccaaa accgtttcct atcttgattt cttgggtctg
     9121 cacatcgaca ccaaagccaa tatggaaaaa cgctacggca attcgggcat tatcagcccg
     9181 accgattctt ctccggctgt tttggtcgtc ccgaccaatg aagaactgat gattgcctgc
     9241 gacactgccg aacttgccgg catcttgtag ccaaaaaagg gggcgagtcc gcaaaaatgc
     9301 cgtctgaaag gttttcagac ggcattttgt ttggcgtttg ggcgtacaat acggaaaaca
     9361 tgacgataag gaaacaaact atggcacaat ttttcgccgt tcatcccgac aatccccaag
     9421 aacgcctcat caagcaggca gtcgaaatca tcaatcaagg cggcgtggtg gtttatccga
     9481 ccgattcctg ttacgccttg ggttgcaaac tcggcgataa ggcggcgatg gaacgcatac
     9541 tctccatccg aaaaatcgat ttgaaacacc acctgaccct gatgtgcgcc gatttgagcg
     9601 agttgggcac atacgccaaa atcgacaacg tacagtttcg tcagcttaaa gccgccacgc
     9661 ccgggcctta tacctttatc ttgcaggcga cgaaggatgc gccggcgcgc gccctgcacc
     9721 cgaaacgcaa aaccatcggg ctgcgtattc ccgataatgc cgttgcacaa gccctgctgg
     9781 aggagttggg cggaccgctt ttaagctgca ccctgatgct gcccgaagac ggcgaaccat
     9841 tgaccgaccc ttatgaaatc cgcgagcgtt tggaacacgc cgtcgatttg gtgattgacg
     9901 gcggctggtg cggaaccgat ccgactaccg tcatcgacat gaccgacggc acggaactgg
     9961 tgcgccgggg tttcggcgat acggcggtgt tcggtttgta gggaaaccga tgccgtctga
    10021 agcatcagcc gttcagacgg catcgcgcgc cttgccggcg gcagcccgaa atgccggcgc
    10081 gtatcgcgcc cggtcggaat atccgtttga aacggcaaac aaaatgccgt ccgaacgcgg
    10141 gtttgggcgg cattttgatg cgttatcgca ccgcaatcgg gattttcgat tcataaagca
    10201 ggtcgtaggt cggcttgttg agcaggtctt gcagcgtgaa accgtccaga tacgtgaaaa
    10261 acgacttcat tgcaccgccg agtatgcccg tcagccggca ggacggcgta atcaggcatt
    10321 cgttgttcgg gcccatacac tcgaccagct gcatcggttc gaggtggcgg acgaccgcgc
    10381 cgatattgat gcgttcgggc ggcgcggcca gcctcagccc gccgcctttc ccgcgtacgc
    10441 tgtgcaagaa cccgcctttg accagcgcgg taaccacttt catcaaatgg cttttggaaa
    10501 tgccgtaggt cgaggcgatg gtggcgatat tgaccagcgc gtcgtcgttg acggcggtgt
    10561 agatgaggac gcgcagcccg tagtcggtat gttgggtcag atacatgatt ttctcggtat
    10621 ggatcgttat tcttatcggt acggtttaag tttcacggaa aataccttaa tggttgaaac
    10681 cctgtccgtc ggggcggtag aatgtagcct gtctgcggcg gtatgccgtc tgaaacatcc
    10741 gcgctaccgt ttgataattt gttattttaa cctaaattta tgaaaccgtt gaaacgacat
    10801 tccgcccttg tcgggctttc gcgcgaacac caccactcgc tttccctgtg cgtgcgtatg
    10861 ttgcggacgc ccggggcaga ccatcgtgcc gaactcgagc tgcattttgc cgaactggaa
    10921 acccattttc gcgaagaaga agccaagttt gccccaattt ggcagaatgt cgcgcctgag
    10981 ttgaaaaccc gttttgaagg cgatcacgcc aaactgcggc agatgatggc aagccccgaa
    11041 tgcggtaacg cggcgtggaa taccgctttt gccacaaccc tgcgcgacca cgcgcgcttt
    11101 gaagaacgcg agctgtttcc cgccgtcgaa ccgtttttgc cggcatgatt ccgttttgcg
    11161 gcaaatatat taatgataaa caaggaacac atatgaaatt taccaaacat cccgtctggg
    11221 caatggcgtt ccgcccgttt tattcactgg cggcactgta cggcgcattg tccgtattgc
    11281 tgtggggttt cggctacacg ggaacgcacg agctgtccgg tttctattgg cacgcgcatg
    11341 agatgatttg gggttatgcc ggtctcgtcg tcatcgcctt cctgctgacc gccgtcgcca
    11401 cttggacggg acagccgccc acgaggggcg gcgttctggt cggcttgacc gccttttggc
    11461 tggctgcgcg gattgccgcc tttatcccgg gttggggtgc ggcggcaagc ggcatactcg
    11521 gtacgctgtt tttctggtac ggcgcggtgt gcatggcttt gcccgttatc cgttcgcaaa
    11581 accggcgcaa ctatgtcgcc gtattcgcaa tatttgtgct gggcggtacg catgcggcgt
    11641 tccacgtcca gctgcacaac ggcaacctag gcggactctt gagcggattg cagtcgggcc
    11701 tggttatggt gtcgggcttt atcggcctga ttgggatgag gattatttcg ttttttacgt
    11761 ccaaacggtt gaacgtgccg cagattccca gtccgaaatg ggtggcgcag gcttcgctgt
    11821 ggctacccat gctgaccgcc atactgatgg cgcacggcgt gatgccttgg ctgtcggcgg
    11881 ctttcgcgtt tgcggcgggc gtgattttta ccgtacaggt gtaccgctgg tggtataaac
    11941 ccgtattgaa agaaccgatg ctgtggattc tgtttgccgg ctatctgttt accggattgg
    12001 ggctgattgc ggtcggcgcg tcttatttca aacctgcctt cctcaatctg ggcgtacatc
    12061 tgatcggggt cggcggtatc ggcgtgctga ctttgggcat gatggcgcgt accgcgctcg
    12121 gtcatacggg caattcgatt tatccgccgc ccaaagccgt tcccgttgcg ttttggctga
    12181 tgatggcggc aaccgccgtc cgtatggttg ccgtattttc ttccggcact gcctacacgc
    12241 acagcatccg cacgtcttcg gttttgtttg cactcgcgct gctggtgtat gcgtggaaat
    12301 acattccgtg gctgatccgt ccgcgttcgg acggcaggcc cggttgagta aaaccgccgc
    12361 agatttcggg tctgcggcgg tttgcttttc agacggcatg gcggtcagtt gccgtccagc
    12421 cagcggtcgc gcgtggtttt ggcttcttca aaatagcggt acagggcttc gcggtcgtcg
    12481 gcggtcagga tgtctgccaa aacgtccaac tgtttgccca agccttgaac cagttgcagc
    12541 aggctatctt tgttggcaag gcagatgtcc gcccacacgg cgggatggcc ggaggcgatg
    12601 cgggtgaagt cccgaaagcc cgtggcggcg aatttcagat attcctgtcc gtcggggtgg
    12661 tcgagaatct ggtggacata ggcgaaggcg gtcaggtggg gcatatggga gacggcggcg
    12721 aaaaccgcgt cgtggtgttg cgcgtccatc gtgaaaatgt ccgcaccgac cgcgcgccac
    12781 aggttttcta ccaatgcgat gccgtctgaa tcttcgccgc cgtggggcgt gatgatgagt
    12841 tttctgtggc ggaacagccc gaactgcgcg gcttgcgcac cgcttctgtc cgaaccggca
    12901 atcgggtgtg cggcgatgca gcggtgcagg cggtcgggca ggcagcggcg gaaggcttcg
    12961 atgaccgaag atttggtgct gccgacatcg gaaatccaag tgtgttccgg caaaacgggg
    13021 cgcagcgcgg tcaaaacggc gggaacggtg gcgacgggcg tggcaatcag taccaagtcc
    13081 gcaccgccga tgctgtccgc gtcgatgacg acggacgcac ggtcaatcac gccgcgttcc
    13141 aatgcacgtt cgaggttgtc gcggtcggtg tcgataccgg taacggtgcg gacgagtccc
    13201 tgccttttga ggtcgagaac gaacgaaccg ccgatcagcc ctacaccgat gagggcaata
    13261 tggttcaaaa tgggcatttg tgtaaacggt tttcgcaaag tgccgccatg gtagcctatc
    13321 ggcggaatat accgcaaggt cggcaggaaa aaggagaaga aatggacaaa atcagagttg
    13381 ccgccgtgca gatggtgtct ggcgtgtcgc cggaaaccaa cgtcgccgcc atgaaacgcc
    13441 tggtcgcacg ggcggcggag cagggtgcgg attgggtgct gctgcccgaa tattgggtgc
    13501 tgatgggcgc aaacgatacc ggcaaactcg cacttgccga gcctttgggc ggcggacgct
    13561 ttcagacggc attgagcgaa acggcgaaag aatgcggcgt ggtgctgttc ggcggaaccg
    13621 tgccgctgca aagccccgaa gcgggcaagg tcatgaatac tttattggtg tacgggtgcg
    13681 acggcgtaag gacggggctg taccacaaaa tgcacctctt cggtttttcc ggtttgggcg
    13741 aacgctatgc cgaagccgat accatccgcg cagggcggga agtgccgcac ttgtcggcag
    13801 aaggcatgcc ggtggcggca ggtatttgtt acgatgtccg ctttcccgaa tttttccgcc
    13861 gccaactgcc gtttgacgta ttgatgctgc cggcggcatt tacccatacg acgggcaagg
    13921 cgcactggga gctgctgctg cgcgcgcgtg ccgtcgaaaa ccaatgttac gtcgtggcgg
    13981 cggcacaggg cggtttgcac gaaaacggac ggcgcacgtt cggacacagt atgattgtcg
    14041 atccgtgggg cgacgtgttg gacgtattac ccgaaggcga aggcatcgtt acggcagaca
    14101 tcgatgccaa ccgcctgaac agcgtccgca accgcctgcc cgccttgaaa caccgggttt
    14161 tggatgccgt ctgaaggttc agacggcatc ggtgtcgggg aatcagcggg ccgtagcaac
    14221 cgaaccgtca ttcccacgga agtggg
//
LOCUS       DAXFZR010000058        13784 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000058 DAXFZR010000000
VERSION     DAXFZR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13784
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.058"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..510
                     /locus_tag="ACFJAS_001514"
     CDS             1..510
                     /locus_tag="ACFJAS_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC3982726.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(740..1321)
                     /locus_tag="ACFJAS_001515"
     CDS             complement(740..1321)
                     /locus_tag="ACFJAS_001515"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC3982727.1"
                     /translation="MEHKLPQLPYEPDALSPHPSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPVGGLDLISTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLEGFWEIVNWDEVAKRFAA"
     gene            1484..2890
                     /gene="dnaB"
                     /locus_tag="ACFJAS_001516"
     CDS             1484..2890
                     /gene="dnaB"
                     /locus_tag="ACFJAS_001516"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC3982728.1"
                     /translation="MNDYAAMPPEGREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPMFIDETPNLTALELRARARRLARRFDGRLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            3045..3710
                     /locus_tag="ACFJAS_001517"
     CDS             3045..3710
                     /locus_tag="ACFJAS_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HFC3982729.1"
                     /translation="MCTRKQQGFTLTELLIVIAIAAIMATIALPNMSGWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPAQVKKDGTVNNQCDFGKKGQGMLAFGDKNDNK
                     AYDGDTKDVFLRNVVLNDDINDKRINYTFNHIAFGSSQPKADRVVWTFNQNGTFGYLP
                     DQNLKDNSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCRKNDTRAV
                     CKH"
     gene            3742..4350
                     /gene="pilV"
                     /locus_tag="ACFJAS_001518"
     CDS             3742..4350
                     /gene="pilV"
                     /locus_tag="ACFJAS_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC3982730.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTEPYTPTPSGGDFKLNNL
                     ISKGDLAKTQLDRFGYELKNALPDAAGIHYAVCKDSSGKEPTLSGNTFSPNCDNKANG
                     DTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            4347..5279
                     /locus_tag="ACFJAS_001519"
     CDS             4347..5279
                     /locus_tag="ACFJAS_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC3982731.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAEQQDLRNAATLIVRDARMAGSFGCFNMSEHIKDVVVSNVAQKNYL
                     FLLKKSNANKLIPIEESSNINYRGFLQVGSALIFQYGIDDANASAETTVVSSCGAISK
                     PGKQIPTLEDAKKELKIPDRDKEQNGNIARQRHVVNAYAVGRIDGEGGLFRFQLNEKG
                     EWGNPQLLAKKIKRMRVRYIYVSGCPEDEDAGKEEQFKYTDKFDSSVTPAGVEVLLDS
                     GSEAKIAASSDNIIYAYRINATIRGGNVCANRTL"
     gene            5258..5869
                     /locus_tag="ACFJAS_001520"
     CDS             5258..5869
                     /locus_tag="ACFJAS_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC3982732.1"
                     /translation="MRKQNALTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYAADSKVTFSENCENGLCTAVNVRT
                     NNNGSEEAFGNIVVKGNPAVEAVKRSCPANSAKNSTGLCIDNKGMEYKKGTGSVSKMP
                     RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            5871..6344
                     /locus_tag="ACFJAS_001521"
     CDS             5871..6344
                     /locus_tag="ACFJAS_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC3982733.1"
                     /translation="MEQKGFTLIEMMIVVTILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDDNDILKSKLEIFVLGYKMNPKIAKKYSVSVKFVDAEKPRV
                     YRLVGVPNAGTGYTLSVWMNSVGDGYKCRDATSAQTYSETLSANTGCEAFSNRKK"
     gene            complement(6414..6722)
                     /locus_tag="ACFJAS_001522"
     CDS             complement(6414..6722)
                     /locus_tag="ACFJAS_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HFC3982734.1"
                     /translation="MKDLLSFPSFLLFSCMLAVTCPTRLIGFFALRNRTLSRRAQTVM
                     ETAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLFTVLIGVGSSGISGWLMA
                     "
     gene            complement(6719..7430)
                     /locus_tag="ACFJAS_001523"
                     /pseudo
     CDS             complement(6719..7430)
                     /locus_tag="ACFJAS_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            7596..8048
                     /gene="dut"
                     /locus_tag="ACFJAS_001524"
     CDS             7596..8048
                     /gene="dut"
                     /locus_tag="ACFJAS_001524"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC3982735.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     GTFLVPTGLAIYLANPAYTAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     GSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            8120..9307
                     /gene="dapC"
                     /locus_tag="ACFJAS_001525"
     CDS             8120..9307
                     /gene="dapC"
                     /locus_tag="ACFJAS_001525"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC3982736.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDGLTVNPDNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPNNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPN
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            9463..10242
                     /gene="yaaA"
                     /locus_tag="ACFJAS_001526"
     CDS             9463..10242
                     /gene="yaaA"
                     /locus_tag="ACFJAS_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC3982737.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQNHVRLLSGLYGLMRPLDLMQPYRLEMGTAFANLRGKNLYEFWGGIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            10325..10415
                     /locus_tag="ACFJAS_001527"
     tRNA            10325..10415
                     /locus_tag="ACFJAS_001527"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10359..10361,aa:Ser,seq:tga)
     gene            10493..10579
                     /locus_tag="ACFJAS_001528"
     tRNA            10493..10579
                     /locus_tag="ACFJAS_001528"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10527..10529,aa:Ser,seq:cga)
     gene            10773..11969
                     /locus_tag="ACFJAS_001529"
     CDS             10773..11969
                     /locus_tag="ACFJAS_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3982738.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGRRSWK
                     LSFMQDGRQQTISLGRYPDFSLAEAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMDINPVAQIGSGVFERVKTKNMTALSPSELPRLIDFLE
                     QRNEFAVYAGRVHIHPVTRFCIYWLLLTMTRIREAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMRWVLDQARGLNVNGRFVFESVNFQGHINKESPRVAMRRSGLDTTAHG
                     LRSLARTYLREVLKVYNDVAEKLLAHSLGTRTQTAYNRSELWEERKDAPERWGNDVLR
                     LADNGK"
     gene            complement(12325..12594)
                     /locus_tag="ACFJAS_001530"
     CDS             complement(12325..12594)
                     /locus_tag="ACFJAS_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982739.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(12789..13472)
                     /locus_tag="ACFJAS_001531"
     CDS             complement(12789..13472)
                     /locus_tag="ACFJAS_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFC3982740.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            13597..>13784
                     /locus_tag="ACFJAS_001532"
     CDS             13597..>13784
                     /locus_tag="ACFJAS_001532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982741.1"
                     /translation="MFPPAEITGRGRVVLQQGFAGFGLYFHGVKFRRLNIPFKLPASC
                     RAVKYARVFAAVSADFGG"
BASE COUNT         3447 a         3117 c         3591 g         3629 t
ORIGIN      
        1 atgacaatga ttttaagcat tttaagcctg ttttttatca ttagactgtt atttttagcc
       61 gtctccatta aacatgaaaa agccttgatt gccaaagggg cgaaacaata cggaaaaacc
      121 aattccacgg tgcttgcggc ggttcatacg ctttattatt tggcgtgttt tgtttgggta
      181 tggctttctg acactgcttt taatggcata tccttgattg gtacactgac ggtgatggct
      241 tcatttgtga tattatcatt gattattaag cagttggggg agatttggac ggttaaaatc
      301 tatattttgc caaatcatca aattaatcgt tcgtggttgt ttaaaacatt ccgccacccg
      361 aattattttt taaacatcat acccgaactg attggcatcg ccttattatg tcaagcgtgg
      421 tatgttttat tgattggcct gcccatttat ttgctggtct tatttaagcg tatccgacaa
      481 gaagaacagg cgatggcaac acttttttaa cccgtttcat caattaaaaa tatatcgaca
      541 aagagaaccg tctatcaatt aaatccattc ttatcattgg ggcaggctta ttgtcattgg
      601 gcaagccgtg acaaaagccc gaacctgcaa aacttatcta taaggctgca tcagtgtgta
      661 ttgaaacccc gcctttttga caacaaatgc cgtctgaagc cttcagacgg catttgttcg
      721 gtttggcaaa gccggcggct tatgcggcaa aacgtttggc gacttcgtcc cagttgacga
      781 tttcccaaaa accttccagg tagttggggc ggctgttgcg gtagtcgatg tagtaggcgt
      841 gttcccacac gtcgcaagtc aagagcggcg tgttttcagt ggtcagcggc gtagcggcgt
      901 tggaagtgga aatcaaatcc aatccgccga caggggtttt caccagccac gcccaaccgg
      961 agccgaacgt accggccgcg caggcattga acgcttcttg gaatttttcg aagctgcccc
     1021 atttcgcgtc gatggcggcg gccagttcgc cggcaggctt gccttggcct ttgggcgtga
     1081 aacccagcca gtagaaggtg tggttccaag tttgagccgc gttgttgaac acgccgcccg
     1141 aagatttttt cacaatctct tccaaaggca ggttttcaaa ttcggtgcct ttgatttgat
     1201 tgttcaggtt ggtgatgtag gtttgatggt gtttgccgta gtggaattcc aaagtctctt
     1261 tgctcggatg cggggacaat gcgtccggtt cataaggcag ttgcggcagc ttatgttcca
     1321 ttttgtgctc ctaatgttgt tttaaatgtt gtattttggc agtgttgctg caaacgaatc
     1381 ggcggctcac gcattctacc tgttttgagt gatggaaacc aattaaacct gctttgcgtt
     1441 ataatagaag attgcaattt cggcatgaca gattggatgt accatgaacg attacgcagc
     1501 tatgccgcct gaaggccgtg aggtcggcgc attgtcgctg cctccgcatt cgatggaggc
     1561 ggaacaatcc gttttgggcg gtttgatgct ggaaaatccg gcttgggaca ggattgccga
     1621 tgtggtttcg ggagaggatt tctaccgcca cgaacaccgc ctgattttcc gatccattgc
     1681 caaattaatc aatgagggcc gtcccgcaga tgtgattacg gttcaggaag atttgcagcg
     1741 gaacgaagaa ttggaagcgg caggcggatt tgaatatctg attacactgg cgcaaaacac
     1801 cccgtctgcc gcaaacatcc gccgctacgc cgaaatcgta cgcgagcgtt ccattatgcg
     1861 ccaactcgcc gaagtgggga cggaaatcgc ccgcagcgca tacaatccgc aaggcaggga
     1921 cgcggggcgg cttttggatg aggcggaaaa caaagtgttt caaatcgccg aaagcaccgc
     1981 caaatccaag cagggctttt tggagatgcc cgatttgctg aaagaagtcg tacagcgcat
     2041 cgatatgctt tattcgcgcg acaatcccga tgaagttacc ggcgtaccga cagggttcat
     2101 cgaccttgat aaaaaaacct cgggtctgca gcccggcgac ctgattatcg tcgccggccg
     2161 cccgtctatg ggtaaaaccg cgttctccat caatattgcc gagtatgtcg ccattgaaaa
     2221 acatttgccc gtcgccgttt tctcgatgga aatgggcggg gcgcaattgg tcatgcgtat
     2281 gctcggttcg gtcggacgct tggatcagag cgtcctgaaa acaggcaggc tggaagatga
     2341 acattggggg cgtttgaacg aggcagtcgt caaactttcc gacgcgccta tgtttattga
     2401 cgaaactccg aatctgaccg ccctcgaatt gcgcgcccgc gcccgccgcc tcgcccgccg
     2461 gtttgacggc agattaggct tgatcgtcat cgactacctg caactgatgg cgggatccgg
     2521 ccgttccgac aaccgcgctt cagagcttgg cgaaatttcc cgttccctca aagctttggc
     2581 aaaagaattg caagtcccca tcattgcctt gtcgcaactg agccgcactg tcgaacagcg
     2641 taccgacaaa cgcccgatga tgtccgactt gagggagtcg ggggcaatcg agcaggatgc
     2701 cgacctgatt atgtttatgt accgcgacga atactacaac caggactcac ccatgaaagg
     2761 ccttgccgaa tgtatcatcg gcaaacaccg caacggtccc gtcggtaaaa tcttcctcac
     2821 atggacggga caattcacca aattcgacaa tgctgcctat attcccgagg aggcaaagat
     2881 agaagattaa atggctatat aaaaatttat taggcggaat caggcaaaat cgtttaaaat
     2941 catgcttaga gattgcccta aaaaataaaa cgcggtcttg aggcattttt gcattcagtc
     3001 cgcagataat tgaaaaatac attacagttg acgtcctatt gcttatgtgt acacgaaaac
     3061 aacaaggttt cacgctaaca gagctgctca tcgtgatcgc cattgcagcc attatggcga
     3121 cgatagccct ccccaatatg agtgggtgga ttgcatcacg ccgcattgcc agtcacgcgg
     3181 agcaggttgc caaccttttg cgtttttcca ggggcgaagc cgtccggctc aatctccctg
     3241 tctatatctg tcctgcccaa gttaaaaaag acggtacggt caacaaccag tgcgacttcg
     3301 gcaagaaggg gcagggaatg ttggctttcg gcgacaaaaa cgacaataag gcatatgacg
     3361 gcgatactaa ggatgttttt ctccgcaacg tggtattgaa tgatgatatc aatgataagc
     3421 ggattaatta caccttcaac catatcgctt tcggttcgtc tcagccgaaa gccgaccgtg
     3481 tagtttggac gttcaaccaa aacgggacat tcggctattt gcccgatcag aatctcaagg
     3541 ataattccaa atttgtttat tccgacggtt atatccaaat cgtgctgaca gatgcgagag
     3601 cagtttcaga tgccgataag aaattccgtt cggcggtggt tttgattgac agcagcggca
     3661 gggttgaagt ttgtcgtaaa aacgatacgc gcgccgtatg caaacattaa tgactgtttt
     3721 agtcggtttg ggaaataaaa aatgaagaat aatgattgct tgcgcctgaa aaatccccag
     3781 tccggtatgg cgttgataga agtcttggtt gctatgctgg ttctgaccat cggtattttg
     3841 gcattgttgt ccgtgcagtt gcggacggtc gcttccgtca gggaggcgga gacacaaacc
     3901 atcgtcagcc aaatcacgca aaacctgatg gaaggaatgt tgatgaatcc gaccattgat
     3961 tcggacagca acaagaaaaa ctataatctt tacacggagc cgtacacccc cactccctct
     4021 ggcggcgatt tcaagcttaa taatttgata agtaaggggg atttggcaaa gacccagttg
     4081 gacaggttcg gttatgaatt gaaaaatgcc ttgccggatg cggcaggtat ccattacgcc
     4141 gtctgcaagg attcgtcggg taaagagccg acattgtccg gcaatacttt ttctccaaat
     4201 tgcgataata aggcaaacgg ggatacttta attaaagtat tgtgggtaaa tgattcggca
     4261 ggggattcgg atatttcccg tacgaatctt gaagtgagcg gcgacaatat cgtatatacc
     4321 tatcaggcaa gggtcggagg tcgtgaatga aacgtaaaat gctaaacgta ccaaagggcg
     4381 gttatgatgg tatgaagggt tttaccattg ttgaatttct ggttgcgggc ctgctcagtg
     4441 tgattgtcct gatagcggtc gtatcgagtt actttacatc ccggaaatta aatgatgcgg
     4501 caaacgagcg tcttgccgag caacaggatt tgcggaatgc ggcaacattg attgtccgcg
     4561 atgcaagaat ggcggggagc ttcggttgct tcaatatgtc tgagcatatc aaagatgttg
     4621 ttgtttccaa tgtggcgcaa aaaaactatc tttttttgtt aaaaaagagc aatgcaaata
     4681 aacttatccc catagaggaa tcttcaaata tcaattatcg gggttttctc caggttggta
     4741 gcgcattgat ttttcaatac ggaatcgatg atgctaatgc aagcgccgag actaccgtcg
     4801 tcagcagctg tggcgcaata tcgaaaccgg gcaagcaaat ccctacttta gaagatgcaa
     4861 aaaaagaatt gaagattccg gatcgggata aggagcaaaa tggcaatata gcgcgtcaaa
     4921 ggcatgtggt caatgcctat gcggtcggca ggattgacgg tgagggaggt ttgttccgct
     4981 tccaattgaa tgaaaagggc gagtggggta atcctcagtt gctcgcgaaa aagattaaac
     5041 gtatgagagt gcggtatatc tatgtttccg gttgtcctga agatgaagat gccggcaaag
     5101 aggaacaatt caaatatacg gataaattcg acagctctgt tacgcctgcc ggggtggagg
     5161 ttttattgga tagcggtagt gaagccaaga ttgccgcttc ttcagataat attatttatg
     5221 cttaccgtat caatgcgaca atacgcgggg gaaatgtatg cgcaaacaga acgctttgac
     5281 aggaatcccg acttctgacg gacagagggg gttcgcactg tttatcgtgc tgatggtgat
     5341 gatagtcgtg gcctttttgg ttgtaactgc cgcccagtcc tacaataccg aacagaggat
     5401 cagtgccaac gaatcagaca ggaaattggc tttgtcttta gccgaggcgg ctttgaggga
     5461 gggcgaattt caggttttgg atttggaata tgctgcggat agtaaggtta catttagcga
     5521 aaactgtgaa aacggcctgt gtaccgcagt gaatgtgcgg acaaataata atggtagtga
     5581 agaggctttt ggcaatatcg tggtgaaagg caatcccgcc gttgaggcgg tgaagcgttc
     5641 ttgccctgca aattctgcca aaaattctac cggcctgtgc attgacaata aagggatgga
     5701 atataagaaa ggcacaggaa gcgtcagcaa aatgccgcgt tatattatcg aatatttagg
     5761 cgtgaagaac ggacaaaatg tttatcgggt tactgccaag gcttggggta agaatgccaa
     5821 taccgtggtc gtccttcaat cttatgtagg caataatgat gagcaataaa atggaacaaa
     5881 aagggtttac attgattgag atgatgatag ttgtcacgat actcggcatt atcagcgtca
     5941 ttgccatacc ttcttatcag agttatattg aaaaaggcta tcagtcccag ctttatacgg
     6001 agatggtcgg tatcaacaat gttctcaaac agtttatttt gaaaaatccc caggacgata
     6061 atgatatcct caagagcaaa ctggaaatat ttgtcttagg ctataagatg aatccgaaaa
     6121 ttgccaaaaa atatagtgtt tcggtaaagt ttgtcgatgc ggaaaaacca agggtataca
     6181 ggttggtcgg tgttccgaac gcggggacgg gttatacctt gtcggtatgg atgaacagcg
     6241 tgggcgacgg atacaaatgc cgtgatgcca cttctgccca gacctattcg gagaccttgt
     6301 ccgcaaatac cggctgtgaa gctttctcta atcgtaaaaa atagggttgt tttgccaata
     6361 ccgtctgaag atcaattttc agacggtatt ttttatgggc attttgaaat gaattatgcc
     6421 atcaaccatc ccgagatgcc tgacgagccg acccctatca atacggtaaa caacatagaa
     6481 aaacggcagg ccgcaaaggc ggttaacgcg atggcaataa gttcgtgcgg cttgtcggag
     6541 acaaaatagg gggcaataac ggaaatgagg acgcagcccg gcgcggtttc cataacggtt
     6601 tgggcgcggc ggcttaaggt tcggttacgc aatgcgaaaa agccgattaa ccgggtaggg
     6661 caggtaacgg caagcataca ggaaaacagc aggaatgatg ggaacgacaa taaatctttc
     6721 atttccgccc attccaaaaa taggcggaca acaggccgga cagcgtgccg gcgggaacat
     6781 accacgcgcc gtcaacggtc aggtaaacgg cggatgcgac aaacaggttg acaaaccaaa
     6841 gtcttgatgc ggaaaaactt ttccacattc ccctcagtaa aaccaaaaat acggcaggaa
     6901 acgccatacc gaacccccaa gcggccacat taccgaacac gggccctact gccgcgccga
     6961 gggcggcaaa ccctatccag gtaatgtaga ggataaagca tgcgcccata taaaaaggca
     7021 tattgaatgc aggcaaaccg gctgctttcc gtttttggat ttcggagaat gccatggccc
     7081 ggctttcatc acacataaaa agcagcgcgg gcacggcttt tttcagcggt atttctttca
     7141 tgtgcgtggc aagcgccgcc ccccatcagg atatgccgcg aattaatcat aaaggttatg
     7201 gtggcgatag gcagtatcgg cagaggttcc gcccacaggt tgaccgtggc aaattcggag
     7261 ccgccggcga agttcatccc cgtcatcagc agcatttcca gccggcccat acctttttgc
     7321 ccgccctgca ttccgagtat caatgcccaa ggcaaaagcc caatcagcat gggcgaactt
     7381 tctttgatgc cgcgtatgaa ttcatcacgt ggagaggctg tgtgcgtcat gttaatgttt
     7441 atatatgaag agacggggcg gtatcataca caggcagaat gtttttgaca gtcgaggatg
     7501 gtttcaaaac aaaagcagtt tgttgtgttt cagcgtaaac acggcttgtg tataatctcc
     7561 aatctttgaa accggccgta tgcaggagca ggacgatgaa tattgaagta gaaatgaaag
     7621 tgttggacga gaggatggcg gattttatcc ctgcctatgc gacggagggt tctgcaggtt
     7681 tggatttgcg cgcctgtttg gatgaggaag tcgttttgca gccgggcgga acgtttcttg
     7741 tgccgacagg tttggcaatt tatttggcga atcccgcata taccgccgtt ttgctgcccc
     7801 gttccggctt ggggcataaa cacggcattg tcttgggcaa tttggtcggc ttaatcgact
     7861 ccgattatca gggagaattg aaggtgtcgt tatggaatag gggcagcgaa ccgtttactg
     7921 tcaaaccgtt cgagcgtatc gcacaaatgg ttatcgtgcc ggtcgtacag gcgggcttca
     7981 aacgtgtcga ggaatttgtc ggaagcagcc ggggtgaggg cggcttcggc agtacgggtt
     8041 ctcactaaag atatagaatg ccgtctgaag gacggttcag gcggcatatc ttccgatata
     8101 ccgataaccg gagaaaatca tgaatacctt actcaagcag ctcaaaccat atccctttgc
     8161 ccgactgcgc gaggcgatgc agggcatttc cgcgcctgaa ggcatggaag ccgtcccact
     8221 gcatatcggc gaaccgaaac acccgacacc gaaagtcatt acggatgcgc tgaccgcctc
     8281 attgcgcgag ttggaaaaat atccgctgac tgcgggtctg cctgaactgc gtcaggcgtg
     8341 tgcgaactgg ttaaaacgcc gttatgacgg cttgacggtc aatccggata atgaaattct
     8401 gccggttttg ggcagtaggg aggcgttgtt ttcttttgtt caaaccgtgt tgaaccctgt
     8461 ttcagacggc ctaaaacccg taatcgtcag cccgaatccc ttttatcaga tttatgaagg
     8521 tgcgacactt ttgggcggcg gtgaaatcca ttttgccaat tgccccgcgc cgtctttcaa
     8581 ccccgattgg cgcagtattt ccgaagaggt ttgggaacgc accaaactgg tgttcgtctg
     8641 ctcgcccaac aaccccagcg gcagcgtgcc ggatttggac ggatggagag aagtttttga
     8701 tttacaggat aaatatggtt tcattattgc ctcggatgaa tgctattccg aaatctattt
     8761 tgacggcaac aaacctttgg gctgcctgca ggccgccgca cagtcggggc gaagcaggca
     8821 aaaactgctt atgtttacca gcttgtccaa gcggtccaat gttccgggcc tgcgttccgg
     8881 ttttgtcgcc ggtgatgcgg aactgcttaa gaactttctg ctttacagaa cctatcacgg
     8941 cagcgcaatg agcattcccg tgcagcgtgc cagtattgcc gcctggaatg acgaacaaca
     9001 cgttatcgac aaccgccgcc tgtatcagga aaaatttgag cgcgttattc ccattttgca
     9061 acaggtattt gacgttaaat taccggatgc ctcgttttac atctggttga aagtccccaa
     9121 tggcgacgat ttggcattcg cacgcaattt atggcaaaaa gccgctatcc aagtattgcc
     9181 cggacgcttt ttggcacggg ataccgaaca gggcaatccc ggggaaggtt atgtgcggat
     9241 tgctttggtt gccgatgtcg caacttgtgt caaagctgcg gaaaccattg tttccctata
     9301 tcggtaaaga ataaaaaata ccgtccggac ttttcctgac ggcttttttt aatactatca
     9361 agatgtattt attataacgg tatttacatc acaatcaatc ggcttgcctg aggatgcttt
     9421 taaatctaaa attccgacgt ttgacactat accgagcgga ttatgttttt tgtcctttcc
     9481 cctgcgaaaa accttaacga aaaagaccct tgccctgtca gcgagtttac ccaacccgat
     9541 cttttggcgg aatctgaaat cctgatgcgc caactgaggg agcttgcccc tcaacaaatt
     9601 gcagagttga tgcacgtttc cgacaaaatc gcacttctaa acgccgaacg aaatgcagcg
     9661 tggcacacgc cgtttacgcc ggaaaacgcc aaacaggcag tctttatgtt caacggcgat
     9721 gtttacgaag gtatggatgc aaacacattg aataccaatc aaatacaata tctgcaaaac
     9781 catgtccgcc tgctgtccgg cttgtacggt cttatgcgtc cactggattt gatgcagccc
     9841 taccgcctgg aaatgggaac ggcatttgcc aatttgcgcg gcaagaattt gtatgagttt
     9901 tggggcggca tcattaccaa ccttttaaat gacacgcttg cccaagcagg cagcaatacg
     9961 cttgtcaacc ttgcctcaca ggaatatttc aagtccgtca acacgaaaaa acttcgggcg
    10021 cggctgatta ccccgatatt taaagacgaa aaaaacggta aatataaaat catcagtttc
    10081 tatgccaaac gcgcgcgcgg attgatggtg cgctatgcgg cagaacacaa tattaccgat
    10141 cctgaaatgc tgaaaaattt taattacgaa ggctacgcat tcaatgacgc ggcttccaat
    10201 gaaagcgaat gggtgtttat gcgttcggaa caaataaagt gaaaacaata aattaagtat
    10261 ttcccaaaaa aagtgcttgg caaaatgtat aaacttcatt attattccta ctcttcaaga
    10321 agacggaagc gtggcagagc ggtttaatgc aacggtcttg aaaaccgtcg agggttgata
    10381 gccctccgtg agttcgaatc tcaccgcttc cgccaatcct tgaagtcaaa aaccaaataa
    10441 aaatgaagta gcataaagca tcggcatttt tatttggttt tacttcatta tcggaaacgt
    10501 ggcagagagg ctgaatgcag cggactcgaa atccgctgag ggtgcaaatc ctccgtgggt
    10561 tcgaatcccg ccgtttccgc cgcaaagcaa aaccgccctg attcagggcg gttctttttt
    10621 gttcaggttg tgttaattac tatataaaaa tcatcggttt accctatcat aacgcatcaa
    10681 ggtaaatcat ttgcaatctt gcggcatctt cttattgcat ttttttatgg taatgtatat
    10741 ggtaattttt ggataagtgg gaaattacca taatggcgaa aatcattacg ccgctgtcgg
    10801 caaatcaggt taaaaatgcg aagccgcgcg ataagctgta taagttgtcg gacggcggag
    10861 ggttggcttt gtgggtctat ccgacgggca ggcggagttg gaagctgtcg tttatgcagg
    10921 acggaaggca acagacaatt tcgctggggc ggtatcctga tttctcgctg gctgaggcgc
    10981 gggaatggcg ggaggaggtg cgccgaaaac gggcacacgg ggaaaatgtc gtcaataaga
    11041 aggtgcgggc ggattttgct tttgagaagg tggcgcgtga ttggtttgtg cgttggtcga
    11101 aggggcggtc tgaaaagtat gccggacagg ttatgcggaa ttttgaacgg tgggtttttc
    11161 cggctatcgg caatcttgat atccgtcaag tcaggacggc ggacgtggtc ggctgcctgc
    11221 gtgtgatgga ggcgcgcggt atcgttgata cgttgcgcaa aacgaaaaac agtctgaaga
    11281 tggtgtttgc gtttgcggtc ggttcgggaa tgatggacat caaccctgtc gcgcaaatcg
    11341 gttcgggtgt gtttgaacgg gtgaaaacca aaaacatgac ggcgttgagt ccgtccgaat
    11401 tgccgcgcct gattgatttt ttggagcagc gcaatgaatt tgcggtttat gcgggcaggg
    11461 tgcatatcca tcctgtaacg cggttttgta tctattggct gctgttgacg atgacgcgga
    11521 ttcgggaggc ggcgttgatg gagtggtcgg agttggacgg ggaggtttgg cgtatccccg
    11581 ccgaacggaa aaaggagcgg cgggggcatg atgtgccgct gtcgcgggcg atgcggtggg
    11641 tgttggatca ggcgcggggg ctgaatgtga acgggcggtt tgtgtttgaa agtgtgaatt
    11701 ttcaagggca tatcaataag gaaagtccgc gcgtggcgat gcggcggtcg gggctggata
    11761 cgacggcgca cggtttgcgc tcgcttgcgc gtacttattt gcgcgaggtt ctgaaggtgt
    11821 ataatgatgt ggcggaaaag ctgcttgccc attcgttggg gacgaggacg caaacggctt
    11881 acaaccgctc ggagctttgg gaggagcgta aggatgcgcc ggaacggtgg gggaatgatg
    11941 ttttgaggct tgccgacaac ggaaaatgat ttttttgtgt gttttgcgtt atgtggaaat
    12001 gtagataaga aagctgtttc cgcattatgt gaaaacgccc taatcgggcg tttttttatt
    12061 gctgcaaccc taatgcttcc aaaacttcgc gggtgtccca tacgggggat gcgagggagg
    12121 ttcagacggc acggtatccg tccgtctttt tccatacgca gcagtgtcga gtttgaaatg
    12181 gggtggttgc ggcaggttgc gtaggcaatc agttcgcgga tggtcgggcg gtctatcctt
    12241 gcgcccggtt tggtgatgtt cattttttta ctctcctgtt cggtttaatc ttgcgccgtt
    12301 tttacggctc tgtcgtttgt ctgcctattt cttcttcccg atttttttat atcgcaacaa
    12361 gctttccggc gggaagaata tgtcaaagtc tgttttcaaa tcgggactga cgatgtcggc
    12421 ggcaatcacg ccgtagccgg tggcaaagct gtcgccatca gcgttatgaa taactatatg
    12481 cccgcttttg attagaataa cgcggccgaa tccggatttc cggattccgc cttttttcgg
    12541 ggcggttgca aatgccagtg tttccatccg cccttgtacg tcggaagact ccattgcttc
    12601 gccgaccgct tttttgacgg gcgtttattg aatacttcgt attccatgtc ttcaatcctc
    12661 tttccgttga ttctgatttg tccgggtttc cgctttttta cggcttggca tgcggcaatt
    12721 gcgtctttca tattcggcat atcctttccc caatccgccg aagtttccgc atccgccgcc
    12781 gtctcgattc atgtttgccc tccgatttgt tttacgaagc ccgtaccgtt cacggcatag
    12841 tgtaacttca cttgcttacc cgattccatt cctcgggtta atgcctcata ttccattttt
    12901 gacccttgta ggattgacga tttcgcatct cttgtccttg cttcggccat atccggatat
    12961 tctttctttt tgtatccgtc cataatggct ttttcttcat ctgacatctc aaattttttg
    13021 actgtttccc aagcgctcgc aatccaaccg ccgcaaaatc ggtcggcaag ataagttctg
    13081 tgcgagggct ttcccgcccg gcaggttttc aaaaatttgc ggcggtcggc ggaaatctga
    13141 cgatagacta catcaaaggc ataggctgag gtttccgctc gatttccaat gccgtaaaac
    13201 atcattgttt tcccgcgttg gtaagatttg cacccgaata tatcggcaat catatttgca
    13261 acgccccatt gccagcctgc caatttaaaa gccattttcc gatcggcttt ctgttcggag
    13321 acttttgata aggcgatatc ctcggcattt actttgtact tttccattaa agcctgcgcc
    13381 tgccttaacg cctgtgcggc ttcatgttca tttgccgacc gacccaaagc caaacatttc
    13441 ttgattttgt ctaagacttt ctctttatcc atattaaacc ctttaatttg tccggtattt
    13501 taatttcgca cccttgccca ttcgtctttc gctttgcttt cgattcggtc gggccattcg
    13561 gctagggctg atacgcttac gaactcgggg ctttttttgt ttccgcctgc cgagattacc
    13621 ggccgcggca gggtagtttt gcaacaaggc tttgcaggct tcgggctgta tttccacggc
    13681 gtgaaattca gacggcttaa tataccgttc aagctgcctg cttcctgccg cgccgtcaaa
    13741 tacgcccggg tgttcgccgc agtatcggcg gactttggcg gcaa
//
LOCUS       DAXFZR010000059        13526 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000059 DAXFZR010000000
VERSION     DAXFZR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.059"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(138..347)
                     /locus_tag="ACFJAS_001533"
     CDS             complement(138..347)
                     /locus_tag="ACFJAS_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFC3982742.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCHEYIQLKTK"
     gene            complement(340..561)
                     /locus_tag="ACFJAS_001534"
     CDS             complement(340..561)
                     /locus_tag="ACFJAS_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982743.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            complement(635..922)
                     /locus_tag="ACFJAS_001535"
     CDS             complement(635..922)
                     /locus_tag="ACFJAS_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982744.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            complement(1042..1680)
                     /locus_tag="ACFJAS_001536"
     CDS             complement(1042..1680)
                     /locus_tag="ACFJAS_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982745.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            complement(1677..2051)
                     /locus_tag="ACFJAS_001537"
     CDS             complement(1677..2051)
                     /locus_tag="ACFJAS_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982746.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            complement(2048..3088)
                     /locus_tag="ACFJAS_001538"
     CDS             complement(2048..3088)
                     /locus_tag="ACFJAS_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFC3982747.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            complement(3322..3702)
                     /locus_tag="ACFJAS_001539"
     CDS             complement(3322..3702)
                     /locus_tag="ACFJAS_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3982748.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            3974..4204
                     /locus_tag="ACFJAS_001540"
     CDS             3974..4204
                     /locus_tag="ACFJAS_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC3982749.1"
                     /translation="MEKPLNFKPIPYPQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKFGLKEDADGDE"
     gene            4191..4475
                     /locus_tag="ACFJAS_001541"
     CDS             4191..4475
                     /locus_tag="ACFJAS_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFC3982750.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            4512..5385
                     /locus_tag="ACFJAS_001542"
                     /pseudo
     CDS             4512..5385
                     /locus_tag="ACFJAS_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5378..7144
                     /locus_tag="ACFJAS_001543"
     CDS             5378..7144
                     /locus_tag="ACFJAS_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFC3982751.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGKKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAVIGAEYK
                     APADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTLAASNTL
                     FMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHYPEGVTE
                     GRLEEAFERTRTRCRLKLLKTG"
     gene            complement(7185..7373)
                     /locus_tag="ACFJAS_001544"
     CDS             complement(7185..7373)
                     /locus_tag="ACFJAS_001544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982752.1"
                     /translation="MPSEFFQTAFLPTRFACRVGLPVRPEASDKQGGFYSGLNLNRYG
                     VASPCRTICTVCGFAALS"
     gene            complement(7483..8871)
                     /locus_tag="ACFJAS_001545"
                     /pseudo
     CDS             complement(7483..8871)
                     /locus_tag="ACFJAS_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
     gene            8989..9065
                     /locus_tag="ACFJAS_001546"
     tRNA            8989..9065
                     /locus_tag="ACFJAS_001546"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9023..9025,aa:Arg,seq:acg)
     gene            9083..9157
                     /locus_tag="ACFJAS_001547"
     tRNA            9083..9157
                     /locus_tag="ACFJAS_001547"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9116..9118,aa:Glu,seq:ttc)
     gene            9320..9436
                     /locus_tag="ACFJAS_001548"
                     /pseudo
     CDS             9320..9436
                     /locus_tag="ACFJAS_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9594..10769
                     /locus_tag="ACFJAS_001549"
     CDS             9594..10769
                     /locus_tag="ACFJAS_001549"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC3982753.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(11111..11659)
                     /locus_tag="ACFJAS_001550"
     CDS             complement(11111..11659)
                     /locus_tag="ACFJAS_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC3982754.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(11662..13158)
                     /gene="ccoG"
                     /locus_tag="ACFJAS_001551"
     CDS             complement(11662..13158)
                     /gene="ccoG"
                     /locus_tag="ACFJAS_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC3982755.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
BASE COUNT         3574 a         3382 c         3255 g         3315 t
ORIGIN      
        1 gaaccggttc cgtaccattt gtactgcctg cggcccgccg ccttgtcctg atttttgtta
       61 atccgctatg gtttaaggct ttgtttctta agtccgcaga gtatgccatg gttagacctt
      121 caacgtcgag tgttgtacta ttttgttttt aattgaatat attcgtggca gacaacacag
      181 cgcgtacagc ctcggacggc ttttcgcttt gcttccggta tcgggtcgcc gcagtcttcg
      241 caatggctga agccggcggt gttttcagac ggcggttggt gtttcgccaa ggcttccacc
      301 agaaattcgg cttcgcgttc ggatgcgcgg tcggcaaaat cagtcatgtt tcagacggcc
      361 tttcgtgtgc cagttctgcc agccctcaat ttggatttcg agctttcggt agtatccgcc
      421 atagcggacg gcgtggttta aaagttgttc gggagagccg ccggccggac gctcggggcg
      481 ttcgtattcg accagcagtc cggaaggaac gggcggaatt tccgcgacgg caacggtttt
      541 aatcggggta tccgaaggca cggttgtaga ggtgcaggct gttcgtgcca aggccgttaa
      601 aacggttgcc gtctttgtct gccgcttcac gggtttactc ctgtaaggtg ttgcgcgttt
      661 tgtccaattc gctcagggcg gccgccaaat ccgtgctttg tttttgggaa aagtcgtacc
      721 accgctgcct ttcgtcctgc gcttttttaa gctgcaaggc gtattgttcc gccgcaacca
      781 tagaagaatg ccgataggcg gcaatcaggg cggtttgttc cgcttcggcc ttgtcggcag
      841 cgtaccggta gccgcacgcc cacgcaagca aaagtacaaa tgcccaaagc agccgttttt
      901 tggctcggac aaattcaatc attgccgccc ccttcctgat ttgcggattt tttgatttcc
      961 gccagctgcg gaactgccgc tatgccgcgt ttgattaagg cataattagg aaaggccgtc
     1021 tgaatgggct tttaaagggg gttaaaagag ttcatgggat ggttgcttat ccaaatcagt
     1081 acaaatgaat ttcaggtttt tgttgtatgc aggcaaaggc cattttttcc atacttcatc
     1141 tgcaccaact ttctcaatca gccaatagac caacaaagct gctggctgac tgaaaaaata
     1201 catttcttca gcattttctt ttatccgctt ggcaatatat ttcttctcat tcaaaagtga
     1261 acggatgtca gaaagaatac ttgattggga aatgctggat tgaaatgttt ctagaaaaat
     1321 catattggtt tttttatcaa cttcaggaac aagccctatt tccttttgat aaagctgtga
     1381 taattgggga agaagctctt cttgtggttg atttgcttct ttcaaaattg ccagagtacg
     1441 actaaataag tcatctgtag tttccatcaa agccatgctc ttcgcgactt cgcgctctgc
     1501 ttgctttgga acattgccgt caggcttgta tatattgtca tgaaccagct ctgcataagc
     1561 atgttggagt aaagaacgca cctgaacttc acagcacaaa tcagcaggga tgctgacatt
     1621 ttcgggtgta atgaatgctt gtttaggacg aatctcgtaa tgtttggatt gtatagtcaa
     1681 attgctttgg gattttgctg aatttcatct gcaaaatctt tcgaaacttt agcattccac
     1741 tgagatgagg attcaatgat ttcacaaact atttgaatat gttccgcaag caatgccaca
     1801 aaacgtacgc cgaccaagtc agtcatttga gtttgaggtg aagtgtaatt ttttcggcca
     1861 atcttggcta gagctgaaga aatctctttg actccaggct ttgcttcaat ttttagaaaa
     1921 ttggcaaccg gaacaggaga aatgacattg gataattgat tttgaatttc ttcagccaca
     1981 aatctacccc aagcggaaaa tgccgcttgg tggctttcta aatagctttt gaaatcatca
     2041 atatcactca tttttgttgt tgtaactgtc ctttaattag gacaacagta tattccccat
     2101 cttccgttgg cgcaatttcc atatagtcct gagtatgctc gggaggagtc aagataacta
     2161 caccgttact aaatccgtaa gacctccgtt tgcgtaaacg agttttaata tattcaatat
     2221 ctttacttac ggcattttga ggaaaatcct tttccaccat aaattctgta taagttgttc
     2281 gtttttcttg aggtaaatga ttttgggcaa aattatttac actaatggtt gcttccgcac
     2341 ttttcaatgt aacgcgcaat gcttcatgcg catctaattt tgcatcatca cttaaatcag
     2401 agttatcgat aaaattgcgt gtccactcaa aaaaatcctg cgtcaattta cgggaagaag
     2461 cagctatact catacccaag aatacttgat agaaatagga agctgccggt ctagtttccg
     2521 tttgtgtcat cagatggtca tacaaaaaag ctcgataatt cccagattgt atttgctcta
     2581 gcggccttac tgtttcagcc accaaaaaac ctatcttgaa taatcttgct gaatcggtca
     2641 gtaataattc ttctaagaat tcaatcgtga taaagtcatc ctcttctttg gttcggaacc
     2701 catcttgagg ttcagcctta attacacata aaaacggctt accggtatct ccaaccctac
     2761 cttttaaaac caacaaaata ccacccggtg catttgtatt cagttgtgca tcggtcaaca
     2821 tgtgggcaaa ttgtgcggaa tcctcaataa actctgcttc ctctttgagt tgtatggctg
     2881 ccgctatctg aaaaaaacta ttttcagccg tattgacaac agacatttca atgccatgag
     2941 atttattacc caaagctttg gtcagcctgg tttccaatgt tcttcttgcc gtaataggca
     3001 aatcaatcaa atgtgtactg attttaggtt ctacacgctc tctattttca tttttcggat
     3061 aaatacgatg ggtaataatt tttttcaatt accagccctt caaaagagca attcgtttct
     3121 gtcatcattt ttcctattgt aagttaatta aggtcttttg aaatctgaac cacttggccg
     3181 atgacctgaa tatcggggtg gtttttaata cgctttaaaa tgcacggccg ctcctattgc
     3241 ttaatatctg tttcagaaca ccggagcagc ccgactgtca gagtgtccca aatgccgtct
     3301 gaaatcttca gacggcattt tttatccgtt gctcacttta ccaagcgcag tttgggcttt
     3361 tttctctacc accagcttaa aggcggcgta gtcagcaagc tctttgcggc tgccgctgcc
     3421 gagctgacgg aacagggcaa gcagttcttt ttcttcagca ctcaaggatt cagacggcat
     3481 ggcggctgtt tcgccgcgtc tgccgtgcat gacgtaatca atatcgatgc cgattttaga
     3541 aaaagagaat aatttttcac ttgatgggcg attttcacca cgctcccact ttccccacat
     3601 ctcacgagaa aaaccacatt tttcagcagc ttctgcttgg ttcaagccta atttaattct
     3661 ttcttctttc aatcgcttac caaaaagaga agttttttcc acttaaattt ccttgaaaag
     3721 agaaatattt ttctcataat tcacacatca agacatgaaa caccctaaat catcacgaaa
     3781 aaggacgaaa aaatgaacac tttgaaggct catgcgcgac agctcatgat ggatcacata
     3841 cacccggcta cgggaaaaag cttgtttatt ttaaaacaag ccaagaccct gctgctgcac
     3901 aacagaccgg atgtttatcg tgcaaagagg cctaaaacta aacacattat accacacgaa
     3961 aggcataaat aatatggaaa aacctttaaa tttcaagcct attccctatc cgcaaacgcg
     4021 cggcagcacg gccgtgtggt ttaagcgcaa cggcgtgtgc aagacgcacc gggcgaaaca
     4081 tttcaatttg gagcgcaccg cggtggaaca ccccttgcgc ggcaagctga aaggcaattt
     4141 cggcaaaagc cacgaagcgg cggtaaaatt tgggctgaag gaggatgcgg atggcgacga
     4201 gtaaaaaggc gcagcggctg ttgcgggtgt ttttggcgat ggacgcgcat ccggtaatcg
     4261 gcatcagcaa taaggagctt tcagacggcc tggggctgac gccatcacaa gtcagcaggg
     4321 atattgatga tttggttgca tcgggattgg tcatcaagct tgaaaacggc aactacgcct
     4381 acggcatcaa aaccctacaa atcgcagagc ggttcagaaa acagcaagag cggctgaatg
     4441 cgcgcttgca agaattggaa aaccgaattt actaaatgcg acgacgacgt cgcatttgag
     4501 gagcaaaaga aatggcaaca gaaattttag gacatacggt cggcgtaacg gcaaacgaat
     4561 tggctatcca cagtatggag gttatggaca agttttcagg cggcgaggcc tacaacgaga
     4621 cggtatggat tgagcgcgga cgatttgcgg acgccaaacg atggaaggga tgtttgagcc
     4681 gggacgcgcg ctgatcatca tcaaagagca tacgccgcat gggcggtttg ccgaaatcgc
     4741 tgaaaaagag ttggcctcgg acggcgggaa tcccaaagac tgatgaatgc caccctccgt
     4801 tttatcgacc cgaaaatgaa acaagcgcag cctaagctga tgacgttggg caaatccaaa
     4861 ctgctcgagc tgctggtcga agacgacgac actttgttgg agcttgccga aggcggtgag
     4921 gtcaacggca acacttttga cgatgtcgac cgtatgacta ttaaggagct gcgcgtcgcc
     4981 ctgcgcgaaa gccgtgaaac ggcggaagcg aaagataagg tgattgccga taaaaataaa
     5041 aaggtcgatg agctggcgga aaagctgtcg aaaaagcaga cgggtgtcaa agagcctaaa
     5101 cctgcggatg tgggcatcga gctgacgatg cagcttggca gcttggaagt cggtatccgc
     5161 tcgcaaatca gccgattgcg cgatatgttc gagcagatgg cggcgcacgg cgaggcgcat
     5221 ggatttgacc accgcgcgaa gatggtcggc acgctcaatc aaattatttt ggactgcgag
     5281 caactgcgcg aaagctatgc cctaccgacc gaagcaccgg cagacaatgt gccggaatgg
     5341 ttgggcggtg aaacgggaga aggcgatgaa tccggcaatg attgagcgtc ttaaagcagt
     5401 cgaaaatcag gcggaagcaa tggggcgcgg cgcacgctct gcatatctta agcagcaggc
     5461 gcaggaattg ggcatcagcc ttgccacgct ataccgcaag ctggaggcgg tcagcgtcaa
     5521 gccgacgcgc aaacggcgca gcgatgcggg caagacggag cttaaaccgg aagaagccaa
     5581 attgatttcg gcggttttgg cggaggcgat gaggcgcaac ggcaagcggt tgatgccggt
     5641 gcggcaggcg gtggaaatgc tgcgcgccaa cgggaaaatc gaggcggcgc ggattgacgg
     5701 ggaaaccggg gaagtcatcc ccctttctga aaacaccatt acccgggctt tacgggaata
     5761 caagctgcat tccgaccagc ttttgcagcc cgaccccgtc agccgcatga aatcggaaca
     5821 cccgaaccat tgttggcaaa tcgacccgag tttgtgcgtt ttgtatttcc tgccgcgtca
     5881 gggcaaggat acggggctgc gggtgatgaa ggaagaggag ttttataaaa acaagccgaa
     5941 aaacgtcgtc aaaatcgaaa acgaccgcgt ttggcggtac acggggacag accatgcctc
     6001 cggcacgatt tcggtgcgtt attacttcgg cggcgaaacc agcgcgaacc tctgtgattt
     6061 tttcatctac atgatgcagg cgaaaaaaga catcggcaaa gacccgttcc ggaccgtacc
     6121 gcgcatggtc atgcttgacc cgggcagcgc gaatacttcg gcggcgttta aaaatttgtg
     6181 caagcccttg gatgtgcatg tgcaaatcaa caagccgggc aatccgcgtg ccaaggggca
     6241 agtggaaaaa gccaacgata ttgtggaaac ggcatttgag agcgggttgc gctttaccga
     6301 ggtacacgac atcgaccagc tcaatgcttt atcggaacgc tggatgcgtt actacaacgg
     6361 tacgcaaaaa cacagccgcc acggcatgac ccgctatcag gcgtggaaca aaatcaaacc
     6421 cgagcagctc atcctgccgc cgcctgccga atattgccga gagcttgccg tcagcgcgcc
     6481 gaaagaggcg aaagtctcgg cggatttgga aatccgcttt ggcggacggg tatatagcgt
     6541 aaaaggcatc aaggggattt tggtcggtaa gaaagttttg gtcggtaaga atccttggga
     6601 ggcaaacggg gcgcgggtcg ccacttatga cgcggagggt aacgaggttt gggtatccgt
     6661 acccgaagta gtttttgacg agatgggctt caaggctgat gccgcggtca tcggggcgga
     6721 atacaaagcc ccggccgata cagacgcgca acggcatcgc aaagagctgg acaagctggc
     6781 gatgggtgcg gaaacgctgg aggcggcagc cgccaaacgc aaaggcaagg cagtcccgtt
     6841 cggcggcaaa atcgacccgt acaaacatca agaagatacg cttgccgcaa gcaatacgct
     6901 ctttatgccc aaacaggggc aacagatgga ttacaacaag atggaggtcg ccgagcaggt
     6961 attgagcaag gtcgaaatcg ccaaacgctt aaaaccccgc gtcgaggcag acggcggcga
     7021 ctggaaacag gcagtgtcgg tcatcctcaa acactacccg gaaggtgtga ccgagggcag
     7081 gctggaagag gcgtttgaac gcacccggac aagatgccgt ctgaaacttt tgaaaaccgg
     7141 ttaggtttca accgaaaaca gcttttatag tggattaaca aaaatcagga caaggcggcg
     7201 aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa cgccgtaccg gtttaaattt
     7261 aatccactat aaaaaccacc ctgcttgtcc gatgcctcgg gacggacggg caaaccgaca
     7321 cggcacgcaa accgcgtcgg caaaaatgcc gtctgaaaaa attcagacgg catcttcaga
     7381 cacattacct gcaaacggca acacattcca atccaaaccg atcaggcaat caatacgaac
     7441 ggctgtttac atacttacca gctttcacca accggtatcg atttaaccga tttccttaat
     7501 atttttcctg tccgttttaa acttcgcctt aaacacatcc ggtaaatctt tatcgaaata
     7561 ccaaagcccg tcatccattt ccaatgcgcc gcccattcca tgcagaacga cttttccccc
     7621 gttaaaggat cggtttcggt aatttccacc tcatgcattt ttccccaatc caaaacaggc
     7681 aacttgtgtg caaacgccaa aacttgttcg tcagaacggc cgcacgcctt atcgcatcgg
     7741 cctgaaacat atacgggaca aggctcatcc tctgcataac cgtccggaag gataccggct
     7801 gaggcaggac acaatccgta tgtttccgcc cacgacgaca aagcccgtct cgccgccttt
     7861 ttattggcaa ataatccggt aggcggatta tccgtcacac cgtttttcaa agccgctgtt
     7921 ttcgcattca acatgccgtc tgaacctttt tcaaacctga cggtcgtaaa tgttttaagc
     7981 agatttttgg cagatacata acaacccgaa tgataacgtc cgaccaattc cgctttaatc
     8041 ttatatgcat gcaggctgcc caatgcggaa aaaaaaaaac ggacttcctc cgtattgcac
     8101 caatcaaacg ggggcttttc cggaatccaa caaagccgca acctcgccat atgcccgttc
     8161 aaacgtaccg atataattta cttttccccc gccgtcgaaa caggcaagca cacctgtacc
     8221 gtcgggcaaa ccgtacagcc gttccctcaa ccgttcggga agcgcggcag gaaaaagttt
     8281 cggattcgtc aaacggaaac actgcctgct ccactcctca agcccgtgtg ccgacaggct
     8341 gtattccaaa taatcacaca acgccgatac atccgccatc gcacgatgcc tgtcttccac
     8401 aacaatcccc aacctttcga tgataccgtc caggctgtgc ttgtaaaact gcggatacag
     8461 ccgacgcgaa agctgcacac tgcacaaagc aggcgatgaa aatccgatac ccgcacgatg
     8521 aaactcatgc tttaaaaacg tatagtcgaa acggctgttg tgcgccacca gcacgcaccc
     8581 cttcaatacc gaaaacaacc cgccggcaat ttcagcaaac accggcgcgt cggcaaccat
     8641 gccgtctgaa atccccgtca gttccgccac aaacttcgga atcggttttt gaggattgac
     8701 cagccattca tacctctccg cccttccctg cccaaacttg accaaagcca cttcggttac
     8761 cctgtcttca tacagattgc cgcccgtcga ttccaaatca accacggcaa caggcattcc
     8821 aaaccgtaaa aacgcctttt ccagcaaggg ccagcgaaaa gcaacaatca ttttattctc
     8881 tttaaattca aacaacaaac cgatatttta cacttttaag acatttcatc caacaaaata
     8941 attgacagaa tccgatgatt accataaaat tcaaaaattt attgcagcgc acccgtagct
     9001 cagttggata gagtatctgg ctacgaacca gagggtcggg cgttcgaatc gctccgggtg
     9061 cgccagtaag aaaatacaat acgcgcccat cgtctagcgg ttaggacatt gccctttcac
     9121 ggcggtaacc ggggttcgat tccccgtggg cgtgccagat tccaaatccc cgaaataatc
     9181 cctcggggat ttttattgcc tcggcaattc gttaccatat ctttacctac ccacttcatc
     9241 agaatctcag acgtaatcga atcatattca aacttttgcc atgcaaaccg atatcccgta
     9301 accggatgcg gtgtccgttc aatatagtgg attaaattta aatcaggaca atgcgacgaa
     9361 gccccagaca gtacaaatag tacggcaagg cgaggcaacg ccgtaccggt ttaaatttaa
     9421 tccactatat tttgtccgat tggtacagat ataacagcat attccaaagc tccttctgct
     9481 tttctgttta ccactgcaat atttagagta taataccaaa tttgagtaat ggttctaaaa
     9541 caattaaaac catttttcat gagcctgact gattcgtata ttcggagaaa ctgatgcaga
     9601 atatttttga ccctttgatt attcgtggaa aatcccttat tcccatcgtg caaggcggta
     9661 tgggggtcgg tgtttccgca tcgggtttat ccagcgcggt ggcgcgtgaa aacggcatcg
     9721 gaacgattgc cagtgtagat ttgcgccacc ttcacgaaga cctgctcgcc gaatcccaaa
     9781 tcaatccgag tgaagagaaa tatacgtctt tgaactgtac cgcattagac agggaaatcc
     9841 aaaaagccaa aagtgcttca gagggaaaag gactgattgc ggtcaacgtg atgaaggcgg
     9901 tcaaagacca cgccgcgtat gtccgccagg cttgcgaatc aggggcggat gcggtcgtga
     9961 tgggtgccgg cctgccttta gacctgccgg aaatgaccga gggctatcat aaggatgtcg
    10021 cgctgctgcc gattctgtcc gaatcgcgcg gtattaatat cgtcttgaaa cgttggatga
    10081 aaaaaggcat attgcccgat gcgattgtag tcgaacatcc tgcccatgcg gccggacatt
    10141 tgggtgcatc aaccgttgaa ggcgtaaacg atgccaaatt cgacttcaaa cgcgtgattg
    10201 aggaaacgtt tgaagtgttt aaaaatttgg ggctggaggg agaaaaaatc ccgcttattc
    10261 ttgcgggagg catggcaaat tttgaaaaag tcaaaaccgc cctaaagaac tggggagcct
    10321 ccgccgttca aatcggtacg gcttttgccg ttaccgaaga aggagatgca cacctcaact
    10381 ttaaaaaaac gctcgccagt gcggaaactg aaaaagtagt cgaatttatg tctgttgccg
    10441 gtttgccggc gcgcggtatc cgcaccaaat tcctagacag ctatatcagg cgcgaaggca
    10501 aacttcaggc aaacgccaaa gccgacccgc gccgctgtac ccaaggttta aactgcctga
    10561 ccagctgcgg tctgcgcgac gggctttcca aagcagggca attctgtatt gatatccagc
    10621 ttgctgccgc attccgtgga gaagttgata aggggttgtt tttcagggga aaagaccagc
    10681 ttcccttcgg taatgccatc cgcaccgtcc gcgaaacgat acaatatctg ctgacgggga
    10741 gcgaacctgt tgcaacgctc ggacgctgac atcaggtagg atttgatttg caaacaaaat
    10801 gccgtccgaa gggctttcag acggcgtttt tcaggttgtg ttccggaaca gtgtcgaaaa
    10861 aacggagaga aataaaaacg tttttatatg aacaaaatgc cttccgcatt atgtcggaca
    10921 tacaacacaa atgccgtctg aagcctttca gacggcattt ttcaggctgc ctttcggaat
    10981 gatgttaaaa aataaacggg atgagataca tttattttcg tccgacaaat cagaccatca
    11041 cgccgaataa tcaaacaatg cctgcccgac attgcatata acaaaccaat gcaatgaaaa
    11101 cacggctttt ttatttgctt cctgcattat tgaaaagttt gtccatcggg gtcaaatcga
    11161 ccgcattgcc ctggctggta atccatttgt tctcgacgcg ccacacgcct gccgcgtcct
    11221 ccacgcgcac ataccagtgg ttggccggcg gaagggtttt gaacaccgcc tcatattccg
    11281 ccctgccgtt ctgcgcgctg ccgacgggct tgagggcgac ggtttgatcg tccgccttgc
    11341 gggtcgggtg catcagcagc aggttcaaag gctgtttgcc gtcaaactcg ccgccgacaa
    11401 acacttttgc cgcattcata tcgggagaaa tgaggacctg caccccgata tgccgtctga
    11461 cggcttcttc atcccgatga agctggatgt cgatatgctt gccatcctta taataatcgt
    11521 ccgtaaccaa atctgtcgcg tgctgctgcg cgacaaaaaa catagcgacg ctggcgatga
    11581 cgacaaaaat cggccccgcc atcaagaccc acggccagac gtgtttgtac caaggcttga
    11641 ttggattgtt ttgagacacg gttattctcc gataaaggtt gcgtcttctt ccaaaacaac
    11701 cggcttgccg tctgatgcac cgctttcccg gtattggaag ataaactcga taggatgact
    11761 gcctttgtcc gcgtattccg gaatggtgga cacttggacg ggaagggtta ttgtttcgcg
    11821 cggagccact tttataccgc cttcaggcag cccggtcagg gcgatttcgt caaatccttt
    11881 gacacttgcg gtaatcagct gttctttttc gctgttgttg atgatacgca ggctatatgc
    11941 gttttccagc caacctttgg cgttttcgcg caccattacg ccacggtctt tcagaatatc
    12001 gacttcgacc attttgcgcg tggacaaacc gaccaggaag gcggcgacaa ccactgccaa
    12061 caccgcgccg taacctgcca cgcgcggtct gagcagccgt tttttaatgt ctttttcggc
    12121 atattcgtgt tccagcgcgc tttcggtcgt atagcgtatc aaaccgctcg gataacccat
    12181 tttgtccata atctcatcgc acgcgtcgat acaggcggcg cagccgatac attggtattg
    12241 caggccgttg cggatgtcga tgccgacggg gcagacttgg acgcacatcg cacagttgat
    12301 gcagtcgccc aaacccgcct cttccttgtt gaccgttttc ttgcgggcgc cgcgcggttc
    12361 gccgcgctcc gcgtcataag aaacaatcag cgtgtcctta tcgaacatcg cgctttggaa
    12421 acgtgcgtac ggacacatat gcaggcacac tttttcgcgc ataatatggg cgaagaagaa
    12481 ggtcataaag ccgtaaaacg ctgcggcaaa catcgcgccg ccgcctgctg ctccggtgaa
    12541 taaatcggga acaaactggc ggatggggac aaaccagccg gcaaacgtga tgcccgtcca
    12601 cgcgcagaca aggaaaatca acaggtattt ggtggctttg atgcggattt tggtgaaatt
    12661 ccacggcgat ttttccagct tcaggcgttt gtttctatcg ccttcgacca ggttgtcgat
    12721 ccacagcata atttcggtgt aaaccgtttg cgggcaggaa tagccgcacc acaaacggcc
    12781 ggcaatcgtc gtccaccaaa acagcccgaa ggcgcaaatc atcagcagca gggcaaggta
    12841 aatcaaatcg cccaccccca acgacaatcc gaaaatgaag aaatgccgtt cggggatatt
    12901 gaaaacgacg gcctgccttc cgctccaatt gaaccacgga atgacgtaaa acacaaactg
    12961 ggtcgccaat acggcggcga tacgcagttt ggcgaaccgt ccttccgcct ttttgggatg
    13021 gatgcgttcg ccttcgggat ggatttgaat cacgctggca cgcggatcga acgttttttt
    13081 cactttcggc gcggcttttg tttgttcgga cgtgccgatt ccggatgccg gactgccggc
    13141 ttggttttcc gtggtcattc tgcattcctt agatttttga ttgatggttt gcccgttacc
    13201 gccgccgttt tgcttttcag acgtcatttt tcttgttttt ttaaggcatt gtgtttcaag
    13261 ttttgagaaa atccgctttt cccaaaatat attcccgcta ttgtacaact ttatgtgccg
    13321 tccggatgta ttgggcgaat acatttccca tccgcatcaa aacgccctga ttttaccgca
    13381 ccgcccgaac aaaattaaaa tactcttaaa aaacaaagac taaaaaaaca gacaccccct
    13441 tatcggcaga accgccggcg caagctcata aacaaacgct atcaacaatc cggcacacaa
    13501 tctataacgt tttatttcaa aaggaa
//
LOCUS       DAXFZR010000060        13168 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000060 DAXFZR010000000
VERSION     DAXFZR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13168
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.060"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(205..690)
                     /gene="purE"
                     /locus_tag="ACFJAS_001552"
     CDS             complement(205..690)
                     /gene="purE"
                     /locus_tag="ACFJAS_001552"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC3982756.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(738..1451)
                     /locus_tag="ACFJAS_001553"
     CDS             complement(738..1451)
                     /locus_tag="ACFJAS_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC3982757.1"
                     /translation="MKTKLPLFIIWLSVSASCASVLPVPEGSGTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHTKIHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1451..2119)
                     /locus_tag="ACFJAS_001554"
     CDS             complement(1451..2119)
                     /locus_tag="ACFJAS_001554"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3982758.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2130..2315)
                     /locus_tag="ACFJAS_001555"
     CDS             complement(2130..2315)
                     /locus_tag="ACFJAS_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC3982759.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2459..4435
                     /gene="mutL"
                     /locus_tag="ACFJAS_001556"
     CDS             2459..4435
                     /gene="mutL"
                     /locus_tag="ACFJAS_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC3982760.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4530..6653
                     /gene="dnaX"
                     /locus_tag="ACFJAS_001557"
     CDS             4530..6653
                     /gene="dnaX"
                     /locus_tag="ACFJAS_001557"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC3982761.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDIRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTVSEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5719..5758
                     /gene="dnaX"
                     /locus_tag="ACFJAS_001557"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6734..7069
                     /locus_tag="ACFJAS_001558"
     CDS             6734..7069
                     /locus_tag="ACFJAS_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC3982762.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7722..8768)
                     /gene="recA"
                     /locus_tag="ACFJAS_001559"
     CDS             complement(7722..8768)
                     /gene="recA"
                     /locus_tag="ACFJAS_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC3982763.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8968..9732
                     /gene="aroD"
                     /locus_tag="ACFJAS_001560"
     CDS             8968..9732
                     /gene="aroD"
                     /locus_tag="ACFJAS_001560"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC3982764.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9752..11767
                     /gene="rep"
                     /locus_tag="ACFJAS_001561"
     CDS             9752..11767
                     /gene="rep"
                     /locus_tag="ACFJAS_001561"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC3982765.1"
                     /translation="MMKLNAQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11837..12895)
                     /gene="dinB"
                     /locus_tag="ACFJAS_001562"
     CDS             complement(11837..12895)
                     /gene="dinB"
                     /locus_tag="ACFJAS_001562"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC3982766.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYDARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     PDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
BASE COUNT         3219 a         3832 c         3413 g         2704 t
ORIGIN      
        1 tgtactgtct gcggcttcgt cgccttgtcc tgatttaaat ttaatccact atatatttca
       61 aaaattgaaa cccgcacaga ttgttaaacc ccaaaacagg ggcgggcgaa attcttccgg
      121 caatgggttc atgcaaacaa aatgccatct gaaccccgaa aacagggctt cagacggcat
      181 ttgtgtattc gcggacggcg ggttttaaat ctgttccaat tccatgttta aaacggtttg
      241 ttcctgtttg gctcggaagt ctgccagttt ttgcgccagt tcggggtttt cgttggcgag
      301 catggagatg gcgaacaatg cggcatttgc cgcgcctgcc tcgccgatgg cgaatgtggc
      361 gacgggtacg cctttgggca tttgtacaat cgataaaagc gaatcttcgc cgcgcaggta
      421 tttgcttggg acgggtacgc ccaaaacggg gacggtggtc ttggcggcaa ccatgccggg
      481 taaatgcgcc gcgccgcccg cacccgcgat gatggctttg atgccgcgcg cccgtgcggt
      541 ttcggcgtat tcaaacatca aatccggagt gcggtgtgcg gaaacgacgc gcgcctcata
      601 ttctacgccg aactcttcaa gaaactgcgc tgcctgccgc ataacgggcc aatcgctgtt
      661 gctgcccatg atgatgccga tttgtatcat aaatccttct tggtgcggat ggggtaaaaa
      721 gcggaaaaat ggaaaaacta tcgtttgcgt acggctgcgg cggcgcgttt tgccgccggg
      781 ctgccgggat aggtctgtat caggctgcgc caagtcgccc ttgcaatgtc tttttgctga
      841 agcctgtatt ggcattcgcc gattttgaat atgacttcgg gcgcggttgg gctgtctttg
      901 aaacggttgg cgtaacgccc tccgatttcg atgacagatt cacagttccc catacgcgcc
      961 ctgctttgca gcaacaggta catactgcgt tgcgcgatgc tgccgccgtc tccgccgtcc
     1021 gcccccttta acaaggcggc tgcggcagaa aacctgccgt tttgatagtg tttgagtgcc
     1081 tgattgtaga ggttttgtgc ggtttcgacg gtatgtgcgg atgcgctgcc gccttcggta
     1141 ttgaggtaat gctctttcaa tttgcggtcg tcgagttttt ggacgtatgt cctgccggaa
     1201 gggtgtattt tcgtatgctc caatgctttg actttcccgt ttaacatttc cacttcgttc
     1261 gacagccgga cgattttgcc ttccagatag tccaaacggt cttgcagagt gggaacggga
     1321 tagggaatgc cgtctgaagc attttcctgt gtcggcattt cggttccgct gccctccgga
     1381 acgggtaaaa cggaagcaca ggaggcagac acagacagcc aaatgataaa aagcggtaat
     1441 ttggtcttca ttattttttc agaagcaggg tcaaaccgtc gccgacgggc agggtgatgg
     1501 ggacaatccg cgtatcgttc ggcaggtttt gattgaaatc tttgagaatt ttgacactgg
     1561 gcggcgcatc aaaagccgct tcgcgcatca cccttccgtt caacaaaata ttgtcgatgg
     1621 cgatgatgcc gccttgccgg acgagtttga ggcaacgctc gaaatattgc ggcgtgggcg
     1681 gtttgtctgc gtctatcagt gccaaatcgt agcttccggc ttcaccctgt gcaatcaaat
     1741 catccaatgt cagcaatgca ggttgcaggt gcaggctgat tttatgcgcc acaccggcct
     1801 cgttccaaac ctgacgcgcc gtatcggtaa aggttacatt gatgtcgcag gcggtaatcc
     1861 gcccgtgttc gggcagcgcc aatgcaagcg cggtgctgct gtatccggta aatacgccga
     1921 tttccagata tttttccgcg cgaatcagct ttgccagcca aaccaaaacc gccgcctgtt
     1981 cgcgcgcaat cgccattttg cccatacggt gatgcccggc cttctcacgc agccgcgtca
     2041 aaacgggatg ttcgggttcg ccgatggcat tcaaatagtt ttgcaggtcc ggtgcgacat
     2101 tggacagatg ggtcgtcatt tcggcgggtt cagtcttggt aataggtata aggttttttc
     2161 gccacttttg ccgattcaaa gtttttttgc tcttcgggat tgagttcgac atcccacaaa
     2221 agccctcgat ttttcaaacg ttcttgcgcc aattcaggtt tttcttcgat caggcggttg
     2281 aggaattgtg tggcttcgga ttggtagtgg tacatctttg cgctccaatt ttacgggata
     2341 cggcgtgatt atactggtat tttccaaacg ggataaacgg cttttatcaa gaatacgggc
     2401 agaaagataa ggggttttat tatagaataa ggcgtttttt gcaacggaag accgccttat
     2461 gccacgcatt gccgccctgc ccgaccacct cgtcaaccaa attgccgccg gcgaagtggt
     2521 cgaacgccct gccaacgccc tgaaagaaat cgttgaaaac agcatagacg cgggcgcaac
     2581 ggcggttgat gtggaactgg aaggcggcgg catccgcctc atccgcgtcg gcgacaacgg
     2641 cggcggcatc caccccgacg acatcgaact cgcgctccac cgccacgcca ccagcaaaat
     2701 caaaaccctg aacgatttgg aacacgttgc cagcatgggc tttcgcggcg aaggcttggc
     2761 cagtatcgcc tccgtcagcc gcctgaccct gaccagccgc caagaagaca gttcgcatgc
     2821 gacccaagtc aaagccgaag acggcaaact cagcagcccc accgccgccg cccaccccgt
     2881 cggcaccacc atcgaagccg ccgaactctt cttcaacacc cccgcgcggc gcaagttcct
     2941 caaatccgaa aacaccgaat acgctcactg cgccaccatg ctcgaacgcc tcgcgctggc
     3001 gcatccgcac attgccttct cactcaaacg cgacggcaaa caagtgttca aacttcctgc
     3061 ccaaagcctg catgaacgta tcgccgccat tgtcggcgat gattttcaga cggcatcttt
     3121 ggaaattgac agcggcaata gcgcgctgcg gctctatggc gcgattgcca agccgacttt
     3181 cgccaaaggc aaaaccgata aacaatattg cttcgtcaac catcgttttg tccgcgacaa
     3241 agtcatgctc catgccgtca agcaggcata ccgcgacgta ttgcacaacg ccctcacccc
     3301 tgctttcgtc cttttcctcg agctgccgcc caaagccgtg gatgtcaacg tccacccgac
     3361 caaaaccgaa atccgcttcc gcgacagccg gcaagtacac caactcgtgt tccacacgct
     3421 caacaaagcc cttgccgaca cacgcgccaa cctgaccgaa agcgtcagca acgcaggcga
     3481 agtgttgcat gacattaccg gcgttacgcc tgccccaatg ccgtctgaaa acgacagcga
     3541 aaatctgttt gatagcgcat ccaaccaccc gacaggcaac aaacccgata cacgcaatgc
     3601 ctttggttca tcaggtaaaa ccgcgcccat gccttaccag gccgcgcgtg cgccgcaaca
     3661 acacagcctg tccctgcgcg aaagccgcgc ggcaatggac acctatgccg aactctacaa
     3721 aaaaaccgac gacatcgacc ttgagttgag ccaattcgaa caggcacgtt tcggcaatat
     3781 gccgtctgaa acgcctgctc acaaaacaga tacgccgctt tcagacggca tcccgtccca
     3841 atccgaactg ccgccgctcg gtttcgccat tgcccaatta cttggcatct acattcttgc
     3901 ccaagccgaa gacagcctgt tgctcatcga tatgcacgcc gctgccgaac gcgtcaacta
     3961 cgagaaaatg aaacgccaac gtcaggaaaa cggcaacctg caaagccagc acctgcttat
     4021 tcccgtaacc tttgccgcgt cccacgaaga atgcgccgcc ctcgccgatc atgccgaaac
     4081 gctggcaggc ttcggcctgg aactgtccga catgggcggc aacaccctcg ccgtccgcgc
     4141 cgcgccagta atgttgggca aatccgatgt cgtctccctc gcacgcgacg tattgggcga
     4201 actcgcccaa gtcggcagca gccaaaccat cgcatcacac gaaaaccgca tcctcgccac
     4261 aatgtcctgc cacggctcaa tccgcgccgg ccgcaggctc accctgcccg aaatgaacgc
     4321 gctgctgcgc gatatggaaa atacgccgcg cagcaaccag tgcaaccacg gcaggccgac
     4381 gtgggtcaaa ctgactttga aagaattgga cacactgttc ttgcgcggac agtaagccga
     4441 aaatgctaga atacgccgcc cgaagccgcc gttcagacgg cattccgacg caccgacaga
     4501 aacatcacga ccgaaaccaa gagaaaaaca tggcctatca agttctcgcc cgaaaatggc
     4561 ggcccaaaac cttttccgac ttagtcggtc aggaacacgt cgtcaaagcc ctgcaaaatg
     4621 ccttggacga aggcaggctg caccacgcct acctgctgac cgggacgcgc ggcgtgggca
     4681 aaaccaccat cgcccgcatc cttgccaaaa gcctcaactg cgaaaatgcg caacacggcg
     4741 aaccttgcgg cgtgtgccaa agctgcaccc aaatcgatgc cggacgctat gtcgacctgc
     4801 tcgaaatcga cgccgcctcc aacacaggca tcgacaacat ccgcgaagtc ttggaaaacg
     4861 cccaatacgc gccgaccgcc ggcaaataca aagtctatat catcgacgaa gtgcatatgc
     4921 tttccaaaag cgcgttcaac gccatgctca aaacgctgga agagccgccc gaacacgtca
     4981 aattcatcct cgccaccacc gatccgcaca aagtccccgt taccgtatta agccgctgcc
     5041 tgcaattcgt cttacgcaat atgaccgcgc aacaggttgc cgaccacctt gcccacgtcc
     5101 tcgacagcga aaaaatcgcc tacgaccccc ccgccctgca acttttggga cgtgccgccg
     5161 ccggatcgat gcgcgatgcc ttaagcctgc tcgaccaagc catcgcccta ggttcgggca
     5221 aagttgccga aaacgatatc cgccaaatga tcggcgcggt tgacaaacaa tacctgtacg
     5281 aactgctgac aggcatcgtc aaccaagacg gcgaagccct gctggcaaaa gcgcaggaaa
     5341 tggcggcgtg tgccgtcggc tttgacaacg ccttgggcga actggccata ctgctgcaac
     5401 aactcgccct gatacaggca gtcccctccg ccttggcgca cgacgacccc gattccgata
     5461 ttttgcaccg cctcgcccaa accataagcg gcgaacaaat ccagctttac taccaaatcg
     5521 ccgtccacgg caaacgcgac ctgggcctcg cccccgacga atacgccggc tttatgatga
     5581 ccctgctgcg tatgctggcg ttcgccccct tggcggcggc ttcgtgtgat gcaaacgccg
     5641 tgattgaaaa taccgaactt caatccccgt cggcacaaac cgccgaaaag gaaaccgccg
     5701 caaaaaagcc acaaccgcgc cctgaagcgg atgccgccca aacgcccgtt cagacggcat
     5761 ccgcagcagc aatgccgtct gaaggcaaaa ctgccggccc cgtttcccat caagaaaaca
     5821 acgatgttcc gccttgggaa gacgcgccgg acaaaaccga aaccgcagcc ggcacggcgc
     5881 gaacgtcggc aaaaagcatt cagacggtat ccgaagccga aacgccgccc gaaaatcaag
     5941 tttccaagaa caaggcagcc gacaacgaaa ccgaggcttc cttgtccgaa gtgccgtctg
     6001 aaaaccccat tcaggcaaca ccgaatgatg aagccgttga aacggaaaca tttgcacacg
     6061 aagcccctgc agaacctttc tacggttttc cggataatga ctgccccccg gaagacggcg
     6121 tggaaatccc gccgcccgat tgggcaaatg tcctccctgc cgataccgca ggcggaggga
     6181 cggacgaaga agcagaagcg ggcggcatcg gcggaaacaa cacgccgtcc gccccgccgc
     6241 ccgaattttc caccgaaaac tgggcagcca tcgtccggca cttcgcccgc aaactcggcg
     6301 cggcgcaaat gccggcgcaa cactccgcgt ggacggaata ccgttccgac accggactga
     6361 tggttttggc aatgaccgcc gaagcccgcg ccaccgccga caaaaagcgg ctcgacaaaa
     6421 tccgcgacac cctcgcccaa gcctacgggc tgcaactcac cctgcaaacc gaagactggc
     6481 gcgacgaagc cggccgggaa acccccgcga tgcaggacaa gcgtgtccaa gccgaagaca
     6541 ggcaaaaagc acaagcattg ctcgaagccg accccgccgc acaaaaaatc ctccaagcat
     6601 tcggcgcgca atggcagccc gaatcactgg aattggcggc aaaccggcca taaacagata
     6661 taatgccgcc cgaacccttc ggacggcatt gccgcttccc tcattcattc aaacacaaac
     6721 aggagtattc agtatgttcg gaaaagccgg attaggcggc ctgatgaaac aggcgcagca
     6781 aatgcaggaa aacatgaaaa aggcacaagc caaacttgcc gaaaccgaaa tcgaaggcga
     6841 agcaggcaac ggcttggtca aaatcacaat gacctgcgcg cacgaagtac gcaaaatcga
     6901 catcagcccc gatttgattc aagaagccgc cgatgacaaa gaaatgctcg aagacctcat
     6961 cctcgccgcc ctcaaatccg cccggggcaa agccgaagaa accgcaaaca aaacaatggg
     7021 cgcattcacc caagatctgc cccccggcgt gggcgacttc ttccgctgac cccgaccgtc
     7081 attcccgcga acgcgggaat ccggtaaccg aaaaaccaca ggaatctatc ggaaaaaaac
     7141 agaaaccccc gccgccgtca ttcccgcgca ggcgggaatc tagaacgtag aatctgaaaa
     7201 accgttttac tcgataaatt tccgtgccga caggtctgga ttcccgcctt cgcgggaatg
     7261 acggcatatt ttttgcattt gatataaagg gtcgtttgaa ttttgttcag caagtgcaaa
     7321 gtattgcaca taaaagggcg caggcttcat ccgtaaagtc ggcgcaggat agaggcaaag
     7381 cgggcgtagg tcgggctgta gcaactgtat ttttcacccc gtcgggtaaa aatatagtgg
     7441 attaacaaaa accggtacgg cgttgcctcg ccttagatca aagagaacga ttctctaaga
     7501 tgctgaagca ccaagtgaat cggttccgta ctatagtgga ttaaatttaa accagtacgg
     7561 cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc tctccgcctt gtcctgattt
     7621 ttgttaatcc gctataccaa aactcaaatc aagccgtccg gaggcggctc aaaaaaaacg
     7681 gtacttcgca gcagaagtac cgtttatcgg gatttcaggt tttattcttc gggtcgttcg
     7741 ccgtcggttt cgtcctgcgt cccttcggtg atgtgcattt ctacgccgtt gagggcgcgg
     7801 atttttgcgt cgatttcgtc ggaaatttcg gggttttctt tcagccagac gcggacgttg
     7861 tctttgccct gaccgatttt cgcgccgttg tagctgtacc acgcgccgga tttgttgatg
     7921 atgtcgtttt tcacgccgat gtcgatcaat tcgccttccc aactgatgcc ttctccgtag
     7981 aggatgtcaa actctgcctg acggaacggg ggggcgactt tgtttttgat gactttgacg
     8041 cgggtttcgt tgcccaatac ttcttcgcct tttttgatgg atccggtgcg gcggatgtcg
     8101 aggcggacgg aagaatagaa tttcagcgcg ttgccgccgg tcgtggtctc ggggctgccg
     8161 aacattacgc cgatcttcat tcggatttgg ttgatgaaca caaccagcgt attggttttt
     8221 ttgatgtgtc cggtcagttt gcgcaaagcc tgactcatca ggcgcgcctg cagtccgacg
     8281 tggctgtccc ccatatcgcc ttcgatttcg gctttgggga cgagtgcggc tacggaatcg
     8341 acgactacca tatctatgcc gcccgaacgg acgagcgtgt cgcagatttc caaagcctgt
     8401 tcgccggtat cgggctggga caggtaaagc tcttcgactt ttacgccgag tttgcgggcg
     8461 taaacgggat cgaaggcgtg ttcggcatcg acaaaggcgc acacgccgcc gtttttctgg
     8521 cattgggcga cggcttcgag gcagagtgtg gttttgccgg aggattcggg gccgaagatt
     8581 tcgacgatgc gcccgcgcgg caggccgccg actccgaggg cgaggtctaa tccgagcgat
     8641 ccggtggaaa tgacttcgag gttttcttcc tgctggctgc cgtccatttt catgatggcg
     8701 cctttgccga aacttttttc gatttgcgcc agtgcggcgg caagggcttt gcttttgtcg
     8761 tctgacatgg gggttactcc ggaacaaatg cggtatgtgg gctgcggcgc aacacgggct
     8821 gcggcgcgga atatgaatcg gtcggtcatt atcgcatgtt tgcctttcgg gggctaccat
     8881 ccgcttcaga ccgaatgccg tctgaaacat tgtccctcct ttgctataat ggcgccctta
     8941 cttttcccgc cagccgcttg aaccgccatg cacaccagcc tgaccgtcaa aaataccgtt
     9001 atcggaagcg ggcgcaccaa aatcgccgtg ccgcttgtcg cccgagatgc cgccgattta
     9061 tcctccgtgc tgtcccaaat caaaaacctg cccttcgata tcgtcgagtt ccgcgccgac
     9121 tttttggaat gcgcgggcag catcggcgaa gtattgcgcc acacgcaggc cgtccgcgac
     9181 gcgctgcccg acaaaccgct gctgtttacg ttcagacggc attgcgaagg cggctcgttc
     9241 ccgtgttcgg acgattatta ttttgaactg ctcgacgcgc tgatcgaaag ccgcctgccc
     9301 gacattatcg atatcgagct gttttccggc gaaaccgccg tccgccgcgc cgtggcaaat
     9361 gctcaaaaaa acggcatcgc cgccctgctc tgcaatcatg agtttcaccg cacgccgccg
     9421 caagaagaaa tcgtatgccg tctgaaacag atggaggact gcggcgcgga catctgcaaa
     9481 attgcggtga tgccgcaaag ctcggaagat gtgctgactt tgctttccgc cacgctcgaa
     9541 gcgaaacggc ttgtcgccaa accggttatt acgatgtcga tggggcagac gggtgcggtc
     9601 agccggcttg ccggacaggt gttcggctca agcatcacgt tcggttcggg aacgcaaaac
     9661 tccgcgccgg ggcaaatcgg cgtatccgcc ctccgtgcgg cactcgactg cctcgaaagc
     9721 ggcgcagact gatttcagac ggcatcaaaa catgatgaaa ctcaatgccc aacagctcga
     9781 agccgtccgc tacctcggcg gccccctgtt cgtccttgcc ggcgcaggca gcggcaaaac
     9841 cggcgtgatt acgcaaaaaa tcaagcattt gattgtcaat gtcggctatc tgccgcatac
     9901 cgtcgccgcg attaccttta ccaacaaagc cgctgcggaa atgcaggagc gcgtcgccaa
     9961 aatgctgccc aaaccgcaaa cgcgcgggct gacgatttgc acgttccatt ctttgggcat
    10021 gaagattctg cgcgaagagg cgaaccatat cggttacaaa aaaaacttct ccattctcga
    10081 ctctaccgac agcgccaaaa tcatcggcga actcttaggc ggcacgggaa aagaagccgt
    10141 attcaaggcg cagcaccaga tttccctttg gaaaaacgat ttaaaaacgc ctgaagatgt
    10201 cgttcagacg gcctctaatg tgtgggaaca acaaaccgcg cgcgtgtatg caagttatca
    10261 ggaaaccctg caaagctatc aggcagtaga ttttgacgac ttaatccgcc tgcctgccgt
    10321 gctgttgcag caaaacagtg aagtgcgcaa caaatggcag cggcggctgc gttatctgtt
    10381 ggttgacgaa tgccaagata cgaatacctg ccaatttgcg ctgatgaaac tcttgacagg
    10441 cgcggaaggg atgtttaccg ccgtcggcga cgacgaccag tccatctacg catggcgcgg
    10501 cgcgaacatg gaaaacctgc gcaaaatgca ggaaaactat ccgcagatga aggtcatcaa
    10561 actggagcaa aactaccgct ccaccgcgcg gattctcaaa attgccaaca aagtcatcga
    10621 aaacaacccc aagctgttta ccaaaaaact ttggtcgcaa ttgggcgaag gcgagccggt
    10681 taaagtcgtt gcctgccaaa acgagcagca cgaagccgat tgggtcgtca gccaaatcgt
    10741 caaacaaaaa ctcatcggcg gcgacaaaac ccgatacgcc gattttgccg tgttataccg
    10801 cggcaagcat caggcacgga ttttcgagga agcattgcgc ggcgcgcgca tcccctaccg
    10861 tctctccggc ggacaaagct ttttcgacaa agccgaaatc aaagacgtgt tgtcttatgt
    10921 gcggctgctt gccaacccca acgacgatcc cgcctttctg cgcgccgtta ccacgccaaa
    10981 acgcggcatc ggcgatgtaa cgctgggcaa actcaacgcc tacgcgcacg aacacgaatg
    11041 cagcctgtat gaagccgcac aaaacgaaga agcccttgcc acactgaaca ataccaaccg
    11101 ccaacacctg caagccttta tggatatgtt cggcaactac cgcgccaaag ccgaaatcga
    11161 cgaagcgggc gagttcatca acagcctgct cgaagaaatc gactatgaaa accatttgat
    11221 gcaaaacgaa gaaggcaaag ccggcgaaat caaatggcgc aacgtcggcg agttggtatc
    11281 gtggttcgca cgaaaaggcg aacgggacgg caaaaacgtc atcgaactcg cccaaaccgt
    11341 cgccttgatg acgcttttgg aaggaaaaga cgaagaagaa accgatgccg tctcgctatc
    11401 cacgctgcac gccgccaaag gtttggagta tccgtatgtt ttccttgtcg gttgcgaaga
    11461 aggtgttttg ccgcacaacg acagcatcga agaaggcaac gtcgaagaag aacgccgcct
    11521 gatgtacgtc ggcatcaccc gtgccaaacg ccaactcacg ctgacccact gtgtcaaacg
    11581 caaaaaacaa ggcatatggc aattccccga acccagccga ttcatagacg aaatgccgca
    11641 ggaagatttg aaaatcctgg ggcgcaaagg cggcgaaccg attgtcagca aagaagaagg
    11701 cagacgtaac cttgccgata taatcggaag gatcgacaac ctaaaaaaaa gcggcccggc
    11761 ggattaaacc ggagccgcaa tgccgtctga aagacgttca gacggcatat tttttgaacg
    11821 gcgcgcgtca agcggtttac gcccacaaat cctgctgctg atttttcggt acaagcctgc
    11881 cgacaccgat accgatgagg cggaacgcgt cttccgtctg cggcgggacg cgcgccatca
    11941 acatttgcgc agcctgcagc agagctgcgc agtcgggcaa tacggaggaa taagtcagcg
    12001 tgcgcgtgat gatgcggaaa tcgtaggtct tcagcttgag cgttacgctt tgggcttcga
    12061 cgtttttgcg cgtgatttgc cgccacaggt cttcggcaag atggggcagg tgtccggcag
    12121 cctgcccgag cggcaggtct tcgggcaggg tgatttctgt ggaaatttgg aggcgttcgc
    12181 gttcggcttt gacggggtgt tcgtccgtac cgcgcgccaa atcatagagg cggtagccgt
    12241 agcgtccgaa atgatttaag agctcgccgc gctcgaaacg gcgcaaatcg cccgccgtcc
    12301 gcatacccag cgactgcatt tttttcagcg ttaccttgcc cgctccgggg attttgccca
    12361 aaggcagggt ttccaaaaat gccataactt tgtgcggcgg caacacaaac tgcccgttcg
    12421 gcttgcgcca gtcggaagca attttcgcca gaaatttgtt cggcgcgatg cctgcggatg
    12481 cggtcaaacc cgtttccgca aaaatggcgg cacggatttc tttggcaact tcgccggcgt
    12541 aagggatatt tttgaaatta cgggtaacgt caagataggc ttcgtccggc gacaggggtt
    12601 cgattaaatc ggtataacgt ctgaatacgg cgtgaatctg cgcagaaacc tgacggtaca
    12661 aatcgaaatg cggcggcaca tacaccgctt gcggacacag ccttttcgcc gttgccaccg
    12721 acatcgcgga atgcagcccg aactgccgtg catcatacga tgcggcacaa atcaccgaac
    12781 gcgcgccctc ccacgcgacg accaccggcc gccctttcaa atgcggctgt tcgcgcagtt
    12841 ctaccgatgc gtagaacgcg tccatatcga tgtggatgat tttgcgtaaa gacatcggct
    12901 ctgccgagga taaaagggat attttactgc gggcatcgga caaaatccaa atatactaac
    12961 aggttaaata caaaagagtt ggctggatta attttgaaac tcgataaaaa taatggtttt
    13021 tatcaagcag aaagaaacag taatggaaat tatgatatcg taagaaaaaa ttatctgaaa
    13081 aatattacca aaatacaatc aaatatattg atttacttag ataataccag taccggattt
    13141 aaaattcaac agaggagaaa acaaatca
//
LOCUS       DAXFZR010000061        13058 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000061 DAXFZR010000000
VERSION     DAXFZR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.061"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..81)
                     /locus_tag="ACFJAS_001563"
     CDS             complement(<1..81)
                     /locus_tag="ACFJAS_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3982767.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTA"
     gene            complement(189..383)
                     /locus_tag="ACFJAS_001564"
     CDS             complement(189..383)
                     /locus_tag="ACFJAS_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC3982768.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(373..2454)
                     /locus_tag="ACFJAS_001565"
     CDS             complement(373..2454)
                     /locus_tag="ACFJAS_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC3982769.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2978..3358
                     /locus_tag="ACFJAS_001566"
     CDS             2978..3358
                     /locus_tag="ACFJAS_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC3982770.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3494..3982)
                     /locus_tag="ACFJAS_001567"
                     /pseudo
     CDS             complement(3494..3982)
                     /locus_tag="ACFJAS_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            4314..5747
                     /locus_tag="ACFJAS_001568"
     CDS             4314..5747
                     /locus_tag="ACFJAS_001568"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC3982771.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEISAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNP
                     TVRKISFTGSTEVGAKIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYVQSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCIDAG"
     gene            5858..6643
                     /locus_tag="ACFJAS_001569"
     CDS             5858..6643
                     /locus_tag="ACFJAS_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC3982772.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            7011..8567
                     /locus_tag="ACFJAS_001570"
     CDS             7011..8567
                     /locus_tag="ACFJAS_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC3982773.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARITGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFAVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8584..9330
                     /gene="surE"
                     /locus_tag="ACFJAS_001571"
     CDS             8584..9330
                     /gene="surE"
                     /locus_tag="ACFJAS_001571"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC3982774.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFYYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLVHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9432..10445
                     /locus_tag="ACFJAS_001572"
     CDS             9432..10445
                     /locus_tag="ACFJAS_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC3982775.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLREYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10843..11325)
                     /locus_tag="ACFJAS_001573"
     CDS             complement(10843..11325)
                     /locus_tag="ACFJAS_001573"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC3982776.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11478..11639)
                     /locus_tag="ACFJAS_001574"
     CDS             complement(11478..11639)
                     /locus_tag="ACFJAS_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982777.1"
                     /translation="MSEQPEKHHASPIEDERKNPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11713..12174)
                     /gene="recX"
                     /locus_tag="ACFJAS_001575"
     CDS             complement(11713..12174)
                     /gene="recX"
                     /locus_tag="ACFJAS_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC3982778.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12247..12957)
                     /locus_tag="ACFJAS_001576"
     CDS             complement(12247..12957)
                     /locus_tag="ACFJAS_001576"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC3982779.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
BASE COUNT         2953 a         3276 c         3721 g         3108 t
ORIGIN      
        1 tgctgttctt gcagttacgc ctgcgacaaa cagttcaatg agtttatttt gtttataccg
       61 gcttagacgg ctttttctca tagggataat tctaacttaa tttgaatttc cctagttatc
      121 taggacagcg ccttattata aaaatgccgt ctgaagtggc gttcagacgg catttgtttc
      181 aagcggggct aacagcagcg tccgccgttt gcgccgcagc ggcgtttgcg gcaatagtct
      241 tgaaattgca gcttgttcat cacgggggcg ttgggattat gtttacgttg ttgtgcaacg
      301 tagttttcat aatcgggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac
      361 caagacgcga gcttatgctt cactttgttc ctccggttgt ttgccgtcgc ggtacaccgc
      421 cgggatttct ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc
      481 gtacgcggca acagtcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt
      541 gtcgttgaag atgatttgcg ccatttcgcc gatgtcttta gcgggcgcaa ggacttcgtt
      601 tttagccaat gcgtcgctgt atttgcctgc gtgggcaagg aagctggcgc gcgggtcgtt
      661 gtggaacagt ttttgcagac cggcgtagca ggttacaagc agtacgccga cggcaggaac
      721 gagtgccacc cagacataac ggtcgcgttt catcttaatc agcacaacgg cacacataat
      781 caaggctacg cctgccagca tttggttggc gatgccgaat aaaggccaga gcgagttgat
      841 gccgcccagc gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa
      901 gaaggtcgca atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat
      961 gctgcccaag tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag
     1021 gatgaacaag gcttcaaaca acagggcgaa gtgataccag aatgccatca tcgcctcgcc
     1081 cggaatcaga cggctcataa tgtgcgccat accgactgcg agggtaggcg caccgcctgt
     1141 gcgggacagg atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcggcatc
     1201 gacagggaag ttcagcttgg tggtaattac ttcggcggcg gtgttggcat ccgtaccgat
     1261 cagtgcggcg gggctgttca tcgcgaaata cacgccggga tcgagcgatg cggcggcggc
     1321 aagcgccata atcgccacga aactttccat caacataccg ccgtaaccga tcatacggac
     1381 gtgggtttcg ttttccagca ttttcggcgt ggtgccggaa gaaatcagtg cgtggaagcc
     1441 cgaaaccgca ccgcaggcga tggtaatgaa caagaacggg aacaatgcgc ctgagaatac
     1501 cgggcccgaa ccgtcgataa agtgggtaac cgcaggcatt tgcaaagcgg gattgacgat
     1561 gacgataccc aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc
     1621 gcgcggggtc agcaagagcc agaccggcaa aaccgaagcc acgaaaccgt aaatcataat
     1681 cgcccaagtg agctggatgc cgtcaaggtc aaaccaatgc ccgatggagc ttttcgccac
     1741 atcttcgccg taaattaccg ccaacatcag caaaataaag ccgacgatgg aaatctcgcc
     1801 gattttgccc ggacggatat ggcgcgtgta aatacccata aacagcgcaa tcggcatcgt
     1861 tgcggcaatg gtgaacgtac cccaagggct gtgaaccaat gcttttacga caatcaacgc
     1921 caaaaccgcc ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg
     1981 gacagtgccg agttcctgtt tcacaatatc gcccaaagac ttaccgtcgc ggcgcataga
     2041 gacgaacaag accgtcatat cctgtaccgc gccggcaaat accacgccga agataatcca
     2101 caaagtaccg ggtagataac ccatttgcgc cgccaaaacc ggaccgacca aagggcccgc
     2161 gccggcaatc gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc
     2221 caagccgtcg ttgtgccgct cggcgggcgt gagacggttc ggatcgagcc gcatcacgcg
     2281 gttggcgatg tagaggctgt aaaaacggta ggcgatacag taaacggata tggcggcggt
     2341 aaccatccat accgcgctga cctgttcgcc tcggctgagg gccagagtgg taaaggatgc
     2401 taagccgacc agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata
     2461 aatccttatc tcacactgtc ggaatatgtc ggagcggaca ggcaacggtt ggctacccgt
     2521 aatccgatgc cgacacaaac cggagtttga atgattgacg aagtatgaaa cagtgcgctt
     2581 gtcgggactt gtgccttatg cccgcatcaa acagtgcagg aatgtacgcg cacaaacgtt
     2641 aaacgcccga ataggatttt aacgcaaatt agcacactga tatcggtttt tacttggaat
     2701 ttggaaaaat ttacattcct ccgggcgggc aggcaggttc agacggcatc gtcaggcaaa
     2761 aggcggtgtc ggaagacggg taaagaaggg gcgcgcaatc cggattattg atttatcgtg
     2821 tttccttttc ggttgaacct cagccctttg gggcggtaaa atcagacttt atttgggagg
     2881 ggcgcaaccc cttccgaatc aggacggcac atagggcggt gctttaatgt gtcgtcctgt
     2941 gtgttgaaac atattttctt tttattgggg agcttttatg aatatcaggt ttttcgcgct
     3001 gaccgtatcg gttttgtctt tggcggcctg tgccgtgccg gaagcgtatg atggcggcgg
     3061 acgcgggtat atgccgcctg ttcaaaacca agccggcccg gacgattttc gagcgttttc
     3121 atgcgagaac ggtttgtctg tgcgcgtccg caatttggac ggcggcaaaa tcgcgttgcg
     3181 gctggacggc aggcgtgccg tcctctcttc cgacgttgcc gcatccggcg aacgctatac
     3241 cgccgaacac ggtttgttcg gaaacggaac cgagtggcac cagaaaggcg gcgaagcctt
     3301 tttcggcttt accgatgcct acggcaattc ggtcgaaact tcctgccgcg cccgttaaac
     3361 ggggcagcct gtttgaaccg ccgtccggat ttttcgggcg gttttttatg ccggttttgt
     3421 tttggccgtc ggctggttcc ggcggttttc gggcgtggcg gattattttt cgtttcgttc
     3481 tgggtttatc ctgtcggcag cggctcaatg ccaactttaa acctgctccg atttcttcag
     3541 ggctgttatc caatgataaa attacatcgt ctgcatcaat ggcatcccac gcttccggct
     3601 tgacatggcg gctcgggctg attttcaggc agccgttgtg cagccaaata tccccgctca
     3661 tcatgttttt aaatagggcg cgtctggttt tatagcccaa gttcccgcat aggttggcaa
     3721 cccaatcctc atagcgttgc cgaaattttt cggtatcaaa aaaatcttgg tcttccgaac
     3781 tgtcataaac gaaagtcctg ctgtttgcca acgcttgcaa gaccgtcgtg cctaaagttt
     3841 cattgtcggt atccaatggc aggatatggg gggatatagg tggtctattg ccgttaaccc
     3901 caaacctgaa catgtttgaa caatcagagc ttcttcatta gcataaaaaa ctgcccaata
     3961 attgatcctg tttaaaaatc attatttaat ctccgtaatt ttgactgtaa tgttttgact
     4021 tttgccatac tctaccacgc gttgtaactg caatctttgc ttcttatata ttgtacccta
     4081 tcaaagggct gcattacttt tctttctaaa gattactatc gcataaaaag tatttttctt
     4141 aaacgacagg gatgcgcccg tgccatattc aaacaccgcc cggatgttgc gctgcccgat
     4201 cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga tgataatgtc
     4261 cgcgagattg acggcatttg aaagcggtta tcccgtatac cggaggacac gaaatgaacg
     4321 aatattccca attaatcaag catcccgata tttccctttc cccgatttca gacggcatcg
     4381 gggtcggcaa tccggcgacg ggcgaaattt cggcgtatgt ccgcaacacg ggttcggaca
     4441 agctgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa attatgggcg gcgaaaaccg
     4501 ccttggaacg cgccgacata ttgtggcgtt ggtatttttt agttaaagaa aacaaagaag
     4561 cactggcgcg tctcatgacg atggagcagg gcaaaagcct gaccgaggcg cgcggcgaaa
     4621 tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg attgacggcg
     4681 atgtgctgac gagtgtgaaa gcgtcgcaaa aactggttgt gttgaaacag cccgtcggcg
     4741 ttaccgctgc gattacgccg tggaatttcc cgtccgcgat gattgcgcgc aaggccgcgc
     4801 ctgctttggc ggtgggctgc gcgatgatag tcaaacctgc atcgctcacg cctttgagtg
     4861 cgtatgcgtt ggctgtactg gcctacgaag cgggcgtgcc gcaggatttg cttcccgtcg
     4921 tcagcggccg cgcttcggaa atcagccatg aatttgccac gaacccgacc gtgcgcaaaa
     4981 tcagcttcac cggctcgacc gaagtcggcg cgaaaatttt tgccggcagc gcggcggaca
     5041 ttaaaaaact cagtttggaa ctgggcggca acgcgccgtt tatcgtgttt gacgatgccg
     5101 atttggacaa agccgtcgaa ggcgcgctgg cgagcaagtt ccgcaacagc ggtcagacct
     5161 gcgtctgcac caaccgcgtt tacgtccaat cgggcattta cgacgtattt tgccgcaaat
     5221 tgagtgaaaa ggcagccgcg ctcaaattgg gcgacggctt ggacgaaggt gtgaaccaag
     5281 gaccgctgat tgaggaaaaa gcggtagaaa aagtcgaaca gcacatcgcc gacgcactct
     5341 ccaaaggcgc ggtctgtctg accggcggca aacgcagcgc gttgggcgga acgttttttg
     5401 agccgactgt cttaagcggc gttacggcgc aaatggcggt agctcgcgag gaaaccttcg
     5461 ggcctttgtg tccggtgttc cgttttgaaa ccgaagccga agtcatcgag gctgcgaaca
     5521 atacggaata cggtttggca gcttaccttt tcacctccga caccgcccgt caatggcgcg
     5581 tcggcgaagt cttggaatac ggcatggtcg gcatcaatac gggcttaatc agtaatgaag
     5641 cggcaccgtt cggcggggtg aaacgcagcg gtttgggacg tgaaggcagc aaatacggtg
     5701 cggacgaata tctagaattg aaatatttgt gcattgatgc cgggtgacgg atgccgtctg
     5761 aatggcaggg tttcagacgg tgtcgcattt taagcagtct ctatctgttg tacaatgcgc
     5821 cctgttttta cggttatttt tgatttgaat gtaagatatg atggaaaacg gaaaaacatt
     5881 cccgagatgg cgttttgcct tgaaaagtgc gggctggcac ctcttaatca gcctattggt
     5941 tgcagggctg gcggcattgc tggtttttaa ggtttggtat ccttatcctt atgccgagct
     6001 gacgggcggt ctgtcgcttt atcagctggt ggtggcggtc gatatcgtat gcggcccgct
     6061 gctgacttta attttggcaa gcccgaagaa aaagacaaag gcacgcatgg tcgatttttc
     6121 catggtcggc atcatccagc tggcggcttt ggcgtacggt ttgcacagcg tttcgctggc
     6181 gcgtcccgtg gtggaagcgt ttgaacagga ccgcatgacc attgttacgg cggcggaagt
     6241 cgtggtcgaa gatttgcaca aagcccccga agggctgcaa agcctgtcgt ggttcggcat
     6301 ccgccgcatt gcattgaaag aacctgagga tgcggatgag aagaacaaga cgctggattt
     6361 gtccctgaaa ggtatcgagc cgagtatgcg tcccgaccgg tggctgccgt attccgacaa
     6421 ggaagcagaa aaaatccgca aacatctgaa accgctgaaa gtcttggcgg atgcgagaaa
     6481 aacgacggtt gcggacattc tgaaacaggc aggtctcgcc gaaggggagg agctgtatta
     6541 cctgccgttt accagcagca ggcagaaaga gtggatagtc attaccgata aagagggcaa
     6601 caccaaaggc tacgcgccga tagacggctt catcatccct taaccattgg gattccgccc
     6661 gcactcgaac atccgttctt cgcggcggta gaatcagact gtatttgaga ggggcgcaac
     6721 ccctcaatca gggcaacaca tagggagacg ctttatgtgc cgtccgtgta ttgaaacatt
     6781 tcaaaataag cccatgccgt ccgaacatcc tttcagacgg catggcgttt tgccattgcc
     6841 ccgataagct gttaaactgt ttaaattatt tcgaccgaag gtacacgcac atgaaagacc
     6901 ccgagcagag cagtaagccc gcccgccgtt ttttgtgcgt accgtctgtt caggcagctt
     6961 gagcgtttca ggctgccttt ttgcgtttga acttgaggaa cacgaaaatc atggatttca
     7021 gttggttggc agaaccgcat acctggatag gttttgccac gcttttggtg ttggaagtcg
     7081 tattggggat agacaacctt gtctttgtgg cgattttggc aaacaaggtc cagcccgcac
     7141 ggcgcgaccg cgcacggatt accgggctgg ggctggcagt cgtcatccgc atcattatgc
     7201 ttgcttttat ggcgcatatc atcacgctga ccgaaccgct gttccaaatc ggcggcctcg
     7261 ccgtttccgg caaggacatg attatgctcg cgggcggtat tttcctgctt tacaaagcca
     7321 ctaccgaact gcacgaacgc ctcgaagggc ataaccggtt tgccgttgcc gacaaccaaa
     7381 aaaaacacgc gccgttttgg ggcgtggtcg cgcaaatcct gatactggat gccgtgtttt
     7441 ccatcgattc ggtcatcacc gccgtggcga tggtcgatca catcgtcgtg gcgatgggcg
     7501 cggtcgtcgt cgcaatggct gtcatgattt ccgccagcaa actcttgacc gaatttgtgg
     7561 acaggcatcc caccgtcgtg atgctctgcc ttggtttttt gttgatgatc ggtttcagcc
     7621 tgattgcgga agccttccat ttccacattc ccaaaggcta cctctacgcc gccatcggct
     7681 tctcgatttt aatcgaactg tttaaccaga tttcgcaacg caacagccgc aaaaacgact
     7741 acatcggcag ctcgtggcgc aagcgcaccg ccgaaaacgt tttgggtatg atgggcatac
     7801 gcgaaagcgt gcttgctgat gcgggcggcg aatccgtgga tgacgcgcat tttgaagaaa
     7861 acgaaaaatc gatgatacgc agcgtgctga cgcttgccga acgcccgatt atgggggtga
     7921 tgattccacg ccgcgacatc gaacggctgg acatttccca aagccgcgaa gaacagtatg
     7981 cccaactgca aaacacgcct tacagccgcc tgctcgttgt cggaaaggcg ggcgtggacg
     8041 aaccgttggg ctacatcaac aaaaaagacc tgctgtccca actgctggaa acaggcggtc
     8101 tcgacattca gacggcattg cgccagccgc tcgtcctgcc cgacggcacg accgccttgg
     8161 gcgcgctcga actcttccgc caaagcagcg cggattacgc tttggtggtg gacgagttcg
     8221 gcgcggtgtt gggtatggtg acgatgaaag acctgttgga agccatcgca ggcgagttcc
     8281 ccgaagaatt cgagcgcgaa gaagaacccg ccgttcaggg gaatcccgat gaaagcctga
     8341 cggtggaagg cgcgttggaa tatgtggagc tggcatccca actcaacctg ccgcagcagg
     8401 aggaagatac cgatttccat accgttgccg ggctgattat ggaagaattg caaaccatcc
     8461 ccgatgtcgg cgattttgcc gattttcacg gctggcggtt tgaagtggtc gaaaaagaag
     8521 ggcagcgcat cgagcgggtc aaaatcacca aattgcccga agaataagca tccaggataa
     8581 aaaatgaacg ttttgatttc caacgacgac ggctatctgg cagaaggcat tgccattctg
     8641 gcgcgggttg cgtcggaatt tgccaatgtc agggtggtcg cgcccgagcg ggacaggagc
     8701 ggggtcagca attcgctgac gctcgaccgt cctttgcagc tgaaacaggc gcaaaacggc
     8761 ttctactacg tcaacggcac gccgaccgac tgcatccatg tcggccagtt tgccctgccc
     8821 gattttaagc ccgatgtcgt gttttcaggt atcaaccgtg gcgcaaatat gggcgacgat
     8881 acgctttatt cggggacggt tgcggcggcg accgaagcct atctgatggg tatgcctgct
     8941 gtggcgtttt ctttaaacga tgcttccgga cgctattggg caaccgcaga aaaggcactg
     9001 tggacattgt tggtgcattt ttttaaaaaa ccgccgtccg cgcccgtctt gtggaatgtc
     9061 aacatccctg ctgtcgcacc ggaagatgtc aggggaatca agattacccg tttgggcaga
     9121 cggcatcatg agcagaacat cgtcccgtcc cgcaatccgc gcggcgaaca gatttattgg
     9181 atagggccgg tcggcgaagt ttccgatcgg gaagagggga cggattttgg tgaatgcggc
     9241 gcaggtttta ttaccgtaac gccgctgcaa atcgatttga ccgcctatcc ggacatggcg
     9301 gagacggcgg ccttttggca tacggactga ccgtttttca aataccgtcg ggttttgcgc
     9361 ttgagaagaa cgcgcatttt tattatgata taccgcattc aggctatata tcggaacatg
     9421 aaggttatct tatgttgaaa caaacgacac ttttggcagc ttgtaccgcc gttgccgctc
     9481 tgttgggcgg ttgcgccacc caacagcctg ctcctgtcat tgcaggcaat tcaggtatgc
     9541 agaccgtatc gtctgcgccg gtttacaatc cttatggcgc aacgccgtac aatgccgctc
     9601 ctgccgccaa cgatgcgccg tatgtgccgc ccgtgcaaac tgcgccggtt tattcgcctc
     9661 ctgcttatgt tccgccgtct gcacctgccg tttcgggtac atatgttcct tcttacgcac
     9721 ccgtcgacat caacgcggcg acgcatacta ttgtgcgtgg cgacacggtg tacaacattt
     9781 ccaaacgcta ccatatctct caagacgatt tccgtgcgtg gaacggcatg accgacaata
     9841 cgttgagcat cggtcagatt gttaaagtca aaccggcagg atatgccgca ccgaaaaccg
     9901 cagccgtaga aagcaggccc gccgtaccgg ctgccgcgca aacccctgtg aaacccgccg
     9961 cgcaaccgcc cgttcagtcc gcgccgcaac ctgccgcgcc cgctgcggaa aataaagcgg
    10021 ttcccgcccc cgcgcccgcc ccgcaatctc ctgccgcttc gccttccggc acgcgttcgg
    10081 tcggcggcat tgtttggcag cgtccgaccc aaggtaaagt ggttgccgat ttcggcggcg
    10141 gcaacaaggg tgtcgatatt gccggcaatg ccggacaacc cgttttggcg gcggctgacg
    10201 gcaaagtggt ttatgccggt tcaggtttga gggaatacgg aaacttggtc atcatccagc
    10261 acaattcctc tttcctgacc gcgtacgggc acaaccaaaa attgctggtc ggcgaaggtc
    10321 agcaggtcaa acgcggtcag caggttgctt tgatgggcaa taccgatgct tccagaacgc
    10381 agcttcattt cgaggtgcgt caaaacggca aaccggttaa cccgaacagc tatatcgcgt
    10441 tctgactttc cgtttgcaat caggttaaaa atgccgtctg aaaggtcttc agacggcatt
    10501 tttgttttgg cggctgtgtt ttcaaatcat tccgaaggga cggggcgtat aaatttgttt
    10561 gactgtgttt gggtatttgc ttgatttcat ccgtttgcaa tggaaaagcc gcttatttcc
    10621 gttcggaggg cggttttatc ggataaaagg cattttgtcc gactgattag ggctgcatca
    10681 agggaatatg aaaacacaac cgcttagtct cacggctgtg ttccaaaatg gcgttgaaaa
    10741 acggatgagc cagaatcagc cgattttact tcaataaatc aaaccattac accgaatgtc
    10801 ggaacaatgc ttgtcctgtc tttcagacgg cataggccgc cgtcagcagc cgcaggacac
    10861 gtctccggag gcttggaggc tgatttctct gcgttttttg gctttttcgt agcggcagcg
    10921 ttggcggtcg gtcaggattt ccagcggagg gacggggacg ggtttgccgt ctttgaccgc
    10981 aaccatcgtg aagtagcagc tgttggtatg gcggatttcg ccggtgcgga tgttttgtgc
    11041 ttcgacacgg atgccgattt ccatcgaggt gcgtcccgtg tagttgacag cggcgtaaaa
    11101 ggtaaccaaa tcgccgatgt ggatgggttc tttaaacagg actttgtcaa ccgaaagggt
    11161 aacgcaataa ttgccgctgt aacggctggc gcaggaatag gcaacctggt cgagcaggag
    11221 caggagttcg ccgccgtgta cgttgccgct gaaattggcg gtgtccggca tcatcagttc
    11281 ggacataatg agttcgtggg aaggcagttg gcgttgttgt gtcatggtgt acctgtttta
    11341 agaaagggtg ttttgaaatg ggtgcgggaa aattttaggg aatggggaag tggaattcaa
    11401 gctaaaaaaa gctatggagg gatagtctgc cgaaatgggc cgccgatgcc gtctgaagcc
    11461 ttcagacggc attttgttca ggaaagccaa aaacggaaaa ccaagaaaaa cacgagtgcc
    11521 agcacgcccg cccaaacggc gagcatgaat ccggcaaccg cccgtcccat acggtaaacc
    11581 gggtttttgc gttcgtcttc gatgggggag gcgtggtgtt tttcgggttg ttcggacatt
    11641 ttgttctcaa tcggaaaagt tggaaacggg cgtaaggtta cgctgccgtg caaaaagatt
    11701 taagaaggaa ggtcagcagc tgtcttccca attttcgtcc caagcgtgtt tcaatgccgt
    11761 ctgaacggta tcggcatcaa aaccgcgata ggcgaggaaa cgcgcctgtt tttgtttttc
    11821 tttgaggttg gcggcgggat gtttgaattt tttacgcaac acggctatgg cggcttgctt
    11881 ttcgcttgag cggtcgggaa gcaggttgcg gctggttttt tcatctatgc cctgttgcgc
    11941 caaagcctgt ttcagcctca atgaaccgtg tttgcggctt ttgctgcgga tataggcttc
    12001 ggcgtagcgc aaatccgact gccagttgcg ttcggcaaat tcgtttaaca cgttttccaa
    12061 ctcctcttcg ctttcggcgt gcggtgcaag tttgcgtttc agaccgatgc ggctgacttc
    12121 ttggcgcgag aggatgtcca tcgcgcgggc gcgtagggat ttttgcggtt tcatggcggc
    12181 ggtttcggca cggaaagatt cagacggtat gggaaacgga ttccgatgcc gtctgaacag
    12241 attgttttat tcttcgtctt tgtttttctg cggtcgcagg ttttcgtaaa tttcgggcag
    12301 cgcgccgata atccggtcgg ggcgggtcgt atcgtcttgc gagagcagcg tcatatcgcc
    12361 gtaaccgaag gtaacgccga cactcaggca gccggcggct ttggcggcga tgatgtcgtt
    12421 gcgcgagtcg ccgaccataa gcatattggc ggcatcgata cccaaaactt cggcggcgtg
    12481 ccgcagcggc agcgggctgg gttttttttc gggcaggctg tctccgccga gtatcaggct
    12541 gaagtagtcg gcgagtccca gttgttttag aagctcggcg gcaaggattt cgtttttgtt
    12601 ggtaatgatt accagcggaa tgcccaagga tttgagcaat gccagcccgg cttcggtttc
    12661 gggatagggg cgggtgaaga cgctcaaatg gtcgcggtag tatttcatat aggacacgaa
    12721 acctttttcc cacagttcgg aatcggcttc gcggtcgcgg tcgttggtga ggacgcggtg
    12781 aaccagtttg ccgatgccgt cgccgacgta gctttcgacc actttggcgg gcagcggttt
    12841 cataccgagt tgttccaaca tcgcttctgc ggcggcggca aggtcgggga cggaatcgca
    12901 cagtgtgccg tccaaatcga aggcgacggc ttggacgtgt tcgatggctg cattcataat
    12961 ccggtttcct gtcgggttga aagggtttat tttaacatcg tttgcacagg gcttctaatg
    13021 tatagattat taacaatcgc aacaggtttt tcgggcgg
//
LOCUS       DAXFZR010000062        12783 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000062 DAXFZR010000000
VERSION     DAXFZR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.062"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..715)
                     /locus_tag="ACFJAS_001577"
     CDS             complement(<1..715)
                     /locus_tag="ACFJAS_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC3982780.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKALKKISPDLVVPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKGKYPDTVFTAL"
     gene            complement(942..1064)
                     /locus_tag="ACFJAS_001578"
     CDS             complement(942..1064)
                     /locus_tag="ACFJAS_001578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982781.1"
                     /translation="MPSETFRRHFPNTPPVAGLHGTQISLIKPQITAAIKPPFK"
     gene            complement(1069..2294)
                     /locus_tag="ACFJAS_001579"
                     /pseudo
     CDS             complement(1069..2294)
                     /locus_tag="ACFJAS_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2688..3935)
                     /gene="fabF"
                     /locus_tag="ACFJAS_001580"
     CDS             complement(2688..3935)
                     /gene="fabF"
                     /locus_tag="ACFJAS_001580"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC3982782.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(4093..4329)
                     /gene="acpP"
                     /locus_tag="ACFJAS_001581"
     CDS             complement(4093..4329)
                     /gene="acpP"
                     /locus_tag="ACFJAS_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC3982783.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            4561..5568
                     /locus_tag="ACFJAS_001582"
     CDS             4561..5568
                     /locus_tag="ACFJAS_001582"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC3982784.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            5765..6238
                     /locus_tag="ACFJAS_001583"
     CDS             5765..6238
                     /locus_tag="ACFJAS_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC3982785.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNILELKPVLEDLASETRGYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENIARLYSHWEHYLNNGGGQG"
     gene            6252..6587
                     /locus_tag="ACFJAS_001584"
     CDS             6252..6587
                     /locus_tag="ACFJAS_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982786.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEAGNQHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            6781..9471
                     /gene="glnE"
                     /locus_tag="ACFJAS_001585"
     CDS             6781..9471
                     /gene="glnE"
                     /locus_tag="ACFJAS_001585"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC3982787.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFIKVGQKLIALLNGITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISLRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            9895..12102
                     /gene="uvrD"
                     /locus_tag="ACFJAS_001586"
     CDS             9895..12102
                     /gene="uvrD"
                     /locus_tag="ACFJAS_001586"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC3982788.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
BASE COUNT         3048 a         3550 c         3462 g         2723 t
ORIGIN      
        1 ccaaagcggt aaaaaccgta tcggggtatt tgcccttaac ctgttccgcc gcccgaataa
       61 aatcatcaat ccctttttct ttcagaaatc tgccgataaa gagaaatttt acgggttctt
      121 tttcatcggg aatatccgcc tcggaataag gatattgccg caaatccaga ccgattccgc
      181 ccaaaatatg gatgtttttt attttgatgc cgtattgatg cagcaactcg tctttgtcgt
      241 cggggtttaa tacaatcagg ctttccaaca tcggcagggt aatgcggtac aaggcgatta
      301 aaatcccctt tatgattttt gtttttaacg gtatgccttc cggctgcggg gtaaatgcga
      361 atcccaaacc ttccagcatc ccgacgattc tgggcacgcc tgccaatttt gcggcaaaag
      421 tgccgaaaat cacgggtttt gcgaaataag ggacaaccaa atccggcgat atttttttga
      481 gtgctttaaa tatgaggaag gtggatttta tatcggaaaa cgggtttagc ccgctgcggt
      541 ttgaccggta ggtaacgggt gtaaccccca tttccctgat aatgtccaac tcattatcgg
      601 aaaactccga tacaaaggca tacacctgat ggtttttgcc gattaatttt ttaatgacgg
      661 gggcgcggaa accgtaaatg ctggatgcga ctgttgtgat aaaaacgatt ttcataaggc
      721 ggacaccttg aatataggat tggaaatgcg gtctgcattg atgaaatgcc ggatacgggg
      781 cgcggcgggc aaacctttgc caagcgcgcc gcatccgacg cttcgcccgc ccgggcaggg
      841 gcggcttcag acggcatttt atcctgaaat ccgctgcata acgcccagcg atatacagta
      901 tcgcgttttg tgatgatttt acacccgaag ggaaaacggg cttatttgaa tggcggttta
      961 atcgcggcgg ttatttgcgg tttgataagg ctgatttgcg tgccgtgcag tccggcaacg
     1021 ggcggggtgt ttggaaaatg ccgtctgaag gtttcagacg gcattctgtc atgcggcggt
     1081 tcggggttcg cggacgggca ggacaagcag ggctgccgtg ccggcgagta cggcatcggc
     1141 gtaccacatc cagccgtagt caccgaattg tgtaatcacg atgccgccga tgtacgagcc
     1201 gaggaatccg ccgatttggt gggtgagcat caccagtccg aacagggtgg cgaggtagcg
     1261 cgtgccgaag agtttgccgg taacggcggc ggtcggggcg acggtggcga gccatgtgaa
     1321 tccgagtgcg gcggcgaaaa tgtaaaagtt gaggtcggtt ttgggtgaga agatgtaaat
     1381 cagcaccatg gcgacgcgtg aggcgtagag tccgaacagg atgtgttttc ctttgaagcg
     1441 tccggtgcat cagcctgaga acacgcaacc ggcgatgttt gccagtccga tgatggcgat
     1501 ggatgtcgag gcgacggtgg cgggcagtcc gcacagggcg acttccgtgg gtaggtgggt
     1561 tacgagaaag gcgatgtgga agccgtaggc gaaaaagctc aggtgcagca ggatgtagct
     1621 tggggttttg aaggcggttt tgactgcttc tccgaggctt tgtccgtgtg tggcttgggt
     1681 gtgttgggtg tgggcggcgt tgttgccgcc ggcaagccac catgagacgg gcagaatcag
     1741 cagggcgatt gcgccccaaa cgtaaaatgt acccgtccag ccgacttcgg gcaggacgac
     1801 gagtccttga gtccttgaac cagcggtgcg aacaggaatt gtcctgccga accgcctgcg
     1861 ttgaccagtc cggatgccaa gccgcgtttg tgtgtgggga cttgggcggc gacctgtccc
     1921 ataatgatgg aaaacccgcc ggaaccggtg ccgaatgcga gcagcagccc gacggcaatc
     1981 atcagccccc aataagtcgg gatgttggag gcaatcaggc aggcgcagac gagcaggagg
     2041 gcaccgccgc ttaataccct gaacgcgccg aaacggtcgg caagcgcgcc ggacaatggt
     2101 tgcaatacgc cccacatcag ttggaaaacg gtgatgatga ggctgaactg ggcaatgttc
     2161 aattcggtgg tgttgacgac gggttggacg aacagtccga gcgtcatcct catgccgatg
     2221 gtaatcagca ggatgaatgc ggcggcggca atgaccgccc agagtttggg tgttttcgat
     2281 gcggtgtgcg tcataatatg tattttgagt cggttaagtt tgcgaatttt aacaaaccgg
     2341 atgattcggg ctaaataatt cctgattctt tggatatagc caacggtatg tctgccgatg
     2401 ccgtctgaaa gggcttgatg ggcgtttgcg gttcgggtta atatttgtgc cgtatttgtt
     2461 aattccagtt agtatagtgg attaacttta aaccggtacg gcgttgcccc gccttagctc
     2521 aaaagagaac gattccctaa ggtgctgaag caccgggcga atcggttccg tactatttgt
     2581 actgcctgcg gcccgccgcc ttgtcctgat ttttgttaat ccactataaa aatgccgtct
     2641 gaaacggttt cagacggcat ttcgatgtcg gcggcggctt tgcggaatca gcctttgaag
     2701 cgtttgaaga ccagcgtacc gttggtgccg ccgaagccga aggagttgga aatggcaacg
     2761 tcgatttccg cgtcgcgcgc ttcgttggcg cagtagtcca aatcgcagcc ggcttcaacg
     2821 tcttgttcaa aaatgttgat ggtcggcggg attttgccgt cgtgtatcgc caaaatgctg
     2881 tacacggcct ccacgccgcc cgccgcgccg agcaggtggc cggtcatgga tttggtcgag
     2941 ctgatgacgg ttttgcaggc gtgttcgccg aacgcgcgtt tgagggcttt ggtttcgttg
     3001 gcatcgccca agggggtgga cgtgccgtgc gcgttgacgt aatccacgtc ttcgggattg
     3061 atgccggcat ctttcagcgc gcgggtaacg gcaagggcgg ggccttcttc gttcggcgcg
     3121 gtgatatggt aagcatcgga actcatgccg aagccgacga tttcggcgta gattttcgcg
     3181 ccgcgttttt tggcgtgttc taattcttcc aacaccaata tgcccgcgcc ttcgccgatg
     3241 acgaagccgt cgcggccttt gtcccacgga cgggaagcgg tggcggggtc gtcgttgcgg
     3301 gtggagaggg ctttcatcgc agcaaaaccg cccacgccca aggtgctgat tgcgccttcc
     3361 gcgccgccgg caaccattat gtccgcgtcg ccgtatttga tcagtcgggc ggaatcgccg
     3421 atggcgtgcg cgccggtggt acaggcggaa accatgccgt agctcgggcc gcggtagcct
     3481 ttgaggatgg taacgtgtcc ggaaatcagg ttgatcagcg aaccggggat aaagaaaggg
     3541 ttgattttgc gcgcgccgcc ttcgattacg gctttgccgg tggcctcgat gctgggcagt
     3601 ccgccgatgc cggaaccgat gttcacgccg atgcggtctt tgtcgaggtt ttccacatcg
     3661 tccaaacccg aatcggcaat cgcctgcaat gcggcggcga tgccgtagtg gatgaacacg
     3721 tccatccggc gcgcttcttt cgcgctgatg tattgtccga tgtcgaaacc gcgcacttcg
     3781 ccggcgacac ggctgttgat gtcggatgcg tcaaagcggg taatcgcgcc gatgccgctt
     3841 ttgccggcga gcaggttgtc ccaagcctct gcggcagtgt tgccgacagg ggaaacctga
     3901 cctaagcctg taatgactac tcttctctga ctcatgataa cctcgctgtt ggttgtcgga
     3961 atgggggggc atatgcggct gtcgtgcaga tgccgtctgt aatttgcggc aggggttcaa
     4021 acagtttgcc atataaggga aaagcctcta ttgcgcggtg cagcagaggc tgttgtgtcg
     4081 ggcgacgacc ggttagccgt tgtgggcatt gatgtagtcg atagccagtt ggacggtggt
     4141 gattttttcg gcatcttcgt cggggatttc gcagccgaag gcttcttcca aagccataac
     4201 cagctccacg gtatccaaag aatccgcgcc caagtcgtct tggaaggaag attcgttttt
     4261 cacgtcggct tcgtttacgc ccagttgttc agcaataatt ttcttaactt gttgttcgat
     4321 gtttgacata tcagtcgttc ctttatgcct tgcggcaggt tgtttaaggg aaataaatcg
     4381 gtggtattgt accgattttt aatagagttt tctatctaat gcctattata tcaatatttg
     4441 cggatttgta catttttggg tgcggcgggt tttgtcgttc aagtttgacc tgtgtgccgt
     4501 atgtttggcg ggatttcggt taaaatggcg gcatttccat ctgaagcaga aagccctgtc
     4561 atgtatccac ttgcccgccg catcctgttt gcactcgatg ccgaaaaagc ccaccacttc
     4621 acgctcgacg cgctcaacac ggtttataaa ttgggcctga ttcccgtaac cgacaaccgt
     4681 accaaaccta taaaactgat gggtatggat ttgcccaacc ctgtcgggct tgccgcaggt
     4741 ctcgacaaaa acggtgaata catcgacgct ttgggcgcgc tcggtttcgg cttcctcgaa
     4801 atcggcacgg ttacgcgcaa cccgcagccc ggcaacccgc agccgcgcct cttccgcgtt
     4861 cccgaacacc aaggcatcat caaccgaatg ggttttaaca accacggcat cgacgcgatg
     4921 atacgcaata ttgaaaaaag caaatatcaa ggcgtattgg gcatcaacat cggcaaaaac
     4981 gcggttacgc ccatccaaaa cgccgccgat gattatttaa tctgcctcga aaaagcctac
     5041 gcgcacgcaa gctacattac cgtcaacatt tcctcgccca acaccaaaaa cctccgcgcg
     5101 ctgcaaggcg gcggcgaatt gggcgcattg ctcgaagccc tgaaaaacaa acaggcgcag
     5161 cttgccgccg cccacggcaa atacatcccg ctagccgtca aaatcgcccc cgatttggat
     5221 gaagcacaaa tcgaagacat cgcccgcgtc gtcaaatccg tcgaaatgga cggcatcatc
     5281 gctaccaaca ccaccatcga caaatcaagc ctcggcagcc atccgctcgc aggcgagcag
     5341 ggcggtttga gcggttttcc cgtgcgcgaa aaaagcaacc gggtgctgaa aaaactggcg
     5401 gaacacatag acggcgcatt gccgattatc ggcgtaggcg gcattatgga aggcggggac
     5461 gcggcagaga aaatccgctt gggcacgacc gccgtccaag tgtacagcgg attgatatac
     5521 aaaggcccgg cgttggtcaa agaatgtttg aaggcattgg cgcgatgacg cgccccaccc
     5581 aaaatgccgt ctgaacgcac gttttgccgt tcagacggca ttttcataga ttggtatccg
     5641 actgttgaag aggcaatcag ccgtcatttg gaagactttt cgtaacaggc gtcaatcgaa
     5701 aggggcggaa tgacggggca ggcaaaccga tgcgtgaaca cggggcaggc aaccggagta
     5761 aaaaatgaac cttgatttaa ccgctcaaaa agtccgcctt tcttggaagg atattctgtg
     5821 ggggtatgag aataaatact tgggttgggc tgatgtggca gcttatgccc gaaaaatgac
     5881 gctttcagat catgatgaac gcgtgttcaa gctatcttta accaataaat ccaatattct
     5941 tgaattaaag cctgttctag aagatttggc ttcggaaacg aggggttatt cccctaaaaa
     6001 ttggctgtac atcctcctaa atgacgtatt ccatagaaaa gaagaatttg aggatcctct
     6061 gggggaagtt gaaaaaattt atgcggattt tgattacccg gaagaaatag aatcatttgt
     6121 caggtatatg ccgcccaaag acggctatat cccttctaat catagttatg aagaaaatat
     6181 tgcccggtta tattctcact gggaacacta tttgaacaac ggcggcgggc agggttaaaa
     6241 ccggcaatcc gatgccgtct gaagcgttat ccggcgttca gacggcattt tgttttccga
     6301 cagtttataa actgtcgttg tttcttgaca gaaacaacga ccttatttat ttgaaacgat
     6361 tggaggacat gattatgggt ttttggggta gtgtgggaaa agcagcaaaa gcagtaggcg
     6421 agggattgat tgaagccggc aatcagcata aggcgttgaa aatggaatat gcggagaaat
     6481 caagtgagga gctgcatgaa atcgtaaaga gtgatggttt ttttaaaaat tccacacggg
     6541 agaaaggtgc ggcttatgct gttttaaaag agcgtggcga ggtttgaacg ggaaacggcg
     6601 gcatttgccg ctgttttttt atcggcaggc atccgtccga atatcggggc aaggtttcag
     6661 acggcatcga aggttgctat gatatagtgg cttgacttta aaccggtacg gctccgcctt
     6721 gccctgattt gaagttcatc cgctatcaca ttcccgccat cctttcaaac ggaatccgaa
     6781 atgtccgacc accgcctcga caccgcccgc cgccattccc tgttcctcgc ccgccagctc
     6841 gacaacggca aactcaagcc cgaaattttc ctgcccatgc tggacaaggc gttgacggat
     6901 gaggatttcc aagcctttgc cgactgggac aaaatccgcg cggaagaaag cgaggaagaa
     6961 ttggcgcggc agttgcgcga gttgcgccgt tatgtggtgt cgcagattat cgtgcgcgat
     7021 ataaaccgta tcagcgattt gaacgaagtt acccgcacga ttacgctgtt tgccgatttt
     7081 gccgtcaata ccgcgctgga tttcgcctac gcctattatc aggacatgta cggcacgccg
     7141 atcgggcgtt ataccaaatc gccgcagcat ttgagcgtgg tggcgatggg caaggcgggc
     7201 ggctatgagc tgaacgtgtc ttccgacatc gatttgattt tcgtctatcc cgaatcgggc
     7261 gacaccgacg gcaggcgcga acgggggaat caggagtttt ttatcaaagt cgggcagaaa
     7321 ctgattgcat tgttgaacgg cattactgcc gacgggcagg tgttccgcgt cgatatgcgg
     7381 ctgcggccgg acggtgattc gggcgcgctg gtgttgagcg aaaccgcgct ggagcaatat
     7441 ctgattacgc aggggcgcga atgggaacgc tatgcgtggt gcaaaggccg cgtagtcacg
     7501 ccgtatccga acgacatcaa atcgctggtg cgccccttcg tgttccgcaa atatctggat
     7561 tacggcgcgt atgaggcgat gcgcaacctg caccgccaaa tccgcagcga agtcagcaaa
     7621 aaaggcatgg cggacaacat caaactcggc gcgggcggca tccgcgaggt tgaatttatc
     7681 gcccagattt tccagatgat acgcggcggc caaatgcgcg cgctgcaact gaaaggcacg
     7741 caggaaacgc tgaagaaaat tgccgagatg ggcattatgc cgtctgaaaa cgtcgaaacc
     7801 ctgcttgccg cctaccgctt cctgcgcgat gtcgaacacc gcctgcaata ctgggacgac
     7861 cggcaaaccc aaaccctgcc gatctcgccc gaacagcggc aactgctcgc cgaaagcatg
     7921 ggtttcgaca gttatgccgc tttttcagac ggcctcaatg ttcatcggaa caaagtcaat
     7981 cagttgttca acgaaatttt gagcgaacct gaagaacaag cgcaaagcaa tagcgaatgg
     8041 caatgggcat ggcaggaaaa acccgacgaa gaagaacggc taggccgtct gaaagaacac
     8101 gggttcgatg ccgaagccgt caccgcaagg ctcgaacaaa tctgccacgg acataaatac
     8161 cgccgccttt ccgcacacgc ccagccgcgc tttgacacca ttgtgccgct gttcgtacag
     8221 gcggcggcag agcaaaacaa cccgacagat acgctgatgc ggctgttgga ttttctcgaa
     8281 aacatcagcc tccgctccgc ctatctcgcc ttcctcaacg aacatccgca aaccttggca
     8341 caactggcgc agattatgag ccaaagctcg tgggtggcgg cgtatctgaa caaatatccg
     8401 attttgttgg acgaactcat cagcgcgcag cttttggata ccgcgtttga ttggcaggca
     8461 ctcgccgccg ccctttcaga cggcatcgaa gcctgcggcg gcgataccga agcgcaaatg
     8521 gacacgctgc gccatttcca acacgcccaa gtcttccgcc tcgccgtcca agacctcgcc
     8581 ggattgtgga cggtagaatc cctctctgac caactctccg ccctcgccga caccgtcatt
     8641 gccgccgccc tttcgtgcgc gtgggcggat atgcccaaaa aacaccgcga caccccgcaa
     8701 ttcgccgtca tcggctacgg caagctcggc ggcaaagaac tcggctacgc ctccgacctc
     8761 gacttagtct atctttacga cgatccccat cccgaagcag gcgacgtgta cagccgcctc
     8821 gcccgccgcc tgaccaaccg gctttccgcc gccaccggcg cgggcagcct ctacgaaacc
     8881 gacctgcgcc tgcgccctaa tggcgacgcc ggtttcctcg cgcacagcat cgccgccttt
     8941 ggaaaatacc agcgtgaaaa cgcatggaca tgggaacacc agtccctcac ccgcgcccgc
     9001 ttcatctgcg gcacacccga aattcagacg gcattcgacc gcatccgcac cgaaatgctg
     9061 actgccgaac gcgaccaaac cgccttggca ggcgaaatca tcgaaatgcg cgaaaaaatg
     9121 ttccccaccc atccgcctgc cgacagcaac gtcaaatacg cgcgcggcgg cgtggtcgac
     9181 gtcgaattta tcgtccaata tctgatactt gcccatgccc gccagtatcc gcaactcttg
     9241 gacaactacg gcaacatcgc cctattgaac atcgccgccg actgcggttt gattgacaaa
     9301 accctcgccg gacaaagccg caccgcctac cgcctctacc gccggcagca gcacaacacc
     9361 aaactgcgcg acgcggcaaa aaccgaagta agcgacgaat tgctgtccca ttacggcaat
     9421 gtcaggaaat tgtggcggga agtgttcggg gaggaagcgg caaccgcctg aacaaaaaat
     9481 gccgtctgaa gcctgacaat ccgggtttca gacggcattt gtttttggct gtgtctgaac
     9541 aatcggtacg atttcggttg tttgcaaatt gaaaggcggt tattcctatg ttcaggcggc
     9601 aaaccgaaat attttcccca accgataggg cgatatcaaa gcctattcga ataccgcctt
     9661 tacggggaag gggctttccg agcttgacca tggtcaggct cggcaaatca gcccgcatcc
     9721 tgtcgatgcg tccgtccaaa tcggtttgga acgccgcaat gtgatatccg cctatcaagg
     9781 ttttgcgtgc gtgtgtcggc gagggctttg ccaaaaccga aatcaaaatc aagttgtcag
     9841 gcatccttcc gtttctatta aaatagcgca ttccattttt cagacggcat ccttatgttt
     9901 cccgaccaat ccgcccccaa tctgctgcaa ggcttgaacc ccgaacaact ctccgccgta
     9961 acctggccgc cgcaatccgc cctcgttttg gcaggcgcag gcagcggcaa aacgcgcgtg
    10021 ctgaccacgc gtatcgcatg gcttttgcaa agcggacaag ccagcgtaca cagcattatg
    10081 gcggtaacgt ttaccaacaa ggccgccaaa gagatgcaaa cccgactcgg cgcaatgatt
    10141 cccatcaacg tccgcgctat gtggctcggc acgttccacg gtttgtgcca ccgctttttg
    10201 cgcctgcacc accgcgacgc aggcctgccg tcttcctttc aaatcctcga cagcggcgac
    10261 cagctttccc tcatcaaacg cctgctcaaa agcctcaaca tcgccgaaga aatcatcgcg
    10321 ccgcgttcgc tgcaaggctt tatcaacgcg caaaaagaat ccggtttgcg cgcttccgtc
    10381 ttgggcgcgc ccgatccgca cacaagccgc atgattgggt gctacgccga atacgacaaa
    10441 atctgccaac gcgaaggcgt ggtcgatttc gccgaactca tgctccgcag ctacgaaatg
    10501 ctgcaaagta acgaaatcct gcgccggcac taccaaaacc gcttcaacca cattctggtc
    10561 gacgagtttc aagacaccaa caaactgcaa tacgcctggc tcaaactcat ggcgggcggt
    10621 aacgcagcgg tatttgccgt cggcgacgac gaccaaagca tttaccgctt ccgcggcgca
    10681 aacgtcggca acatgaccgc actgatggaa gaattccaca tcgacgcgcc cgtcaaactc
    10741 gaacaaaact accgctccgt cggcaacatc ctcgccgccg ccaacgccgt tatcgaaaac
    10801 aacgacgaac gtctcggcaa aaacctgcgc accgacgccg aagcaggcga caaaatccgc
    10861 tactactccg cctttaccga cctcgaagaa gcccggttca tcgtggatga aaccaaagcc
    10921 ctcgaacgcg aaggctggga tttggacgaa atcgccgtcc tctaccgcag caacgcccaa
    10981 tcgcgcgtca tcgaacaaag cctgttccgc agcggcatcc cctacaaaat ctacggcggc
    11041 ctgcgctttt acgaacgcca agaaatcaaa cacgccctcg cctacctgcg cctcgccgtc
    11101 aatcccgacg acgacaacgc cctcttgcgt gtcatcaact tcccaccgcg cggcatcggc
    11161 gcgcgcaccg tcgaaaacct tcagacggcc tcaaacgaac aaggcatcac cctctggcag
    11221 gcagcctgca acgccggcgc gaaagccgcc aaagtcgccg ccttcgtccg cctgattgaa
    11281 gccctgcgca accaagtcgg acaaatgcac ctgtccgaaa tcatcgtcgg catcctcaaa
    11341 gacagcggtc tgaccgagca ctaccgaacc caaaaaggcg acaaccaaga ccgcctcgac
    11401 aaccttgacg aactcgtcaa cgccgccatc gagttcaaac ccgaagacag caactttgaa
    11461 accctgcccg aaaacatttc agacgatccc gccttcccca tcctcgcctt cttgagcaac
    11521 gccgccctcg aatccggcga aaaccaagca ggggcaggcg aaaaagccgt ccaactgatg
    11581 accgtccacg ccgccaaagg cttggaattc aacgccgtct tcctcaccgg tatggaagag
    11641 gggcgtttcc ccagcgaaat gagccttgcc gagcgcggcg gcctcgaaga agaacgccgc
    11701 ctcatgtacg tcgccatcac ccgcgcccgc aaacgcctct acatcaccat ggcacagcaa
    11761 cgcatgctgc acggacaaac ccaattcggc atcgcatccc gcttcgtcga agaaatcccg
    11821 ccagaagtat tgcactacct gtccgtcaaa aaacccgcct tcgacagcta cggcaacacg
    11881 cgccaaacca ccgtacaacg ggacaaaatc atcgatgact tcaaacagcc gcaaacctac
    11941 gcaggcttcc gcatcggaca aaacgtccgt cacgccaaat tcggcaccgg cgtgattatc
    12001 gatgccgcag acaaaggcga atccgcccga ctgaccatca acttcggcaa acagggcgtg
    12061 aaagagctgg ataccaagtt tgcgaaattg gaagcgatgt aaatttgaaa tgcaggccgg
    12121 atattcgtat ccgacaaaaa aacatttgac gcgtttatcg tttccgaaaa accgctgttg
    12181 gaaatgtcgg attcgagaat ccgacctacg gcaaaaaacc gtagcaagga caaggcaaat
    12241 agtcgtctga aaacgggaag ggcaattttg ccgcagccgc cgccgtcatt cccgcgaacc
    12301 tacattccgt cattcccacg aaagtgggaa tccggaacgc aaaatctaaa gaaaccgttt
    12361 tatccgataa gtttccgcac cgacaaaacc agattcccgc ctgcgcggga atgacggcgg
    12421 cgggaatcgg cggattcggc ggagttggtg gatttggcgg gctgaagccc gcccttgtat
    12481 atcggaactt ccgtatcata gcgacaaacc gcccggccac cacccgcgcc cgcccaaggc
    12541 agacaaccgt tgcgtagctc agggagcggc agggcaaccc atcggcacaa ccggacagtt
    12601 gccaactgta tatggctgcc gcggcagcga cacgttttga accgcttatt cgggatttcc
    12661 accaacgcct gctgtatagc ggattaacaa aaaccggtac gggttgcccc gccttagctc
    12721 aaagggaacg attccctaag gtgatgaagc accgggcgga tcggttccgt accattcgta
    12781 ccg
//
LOCUS       DAXFZR010000063        12404 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000063 DAXFZR010000000
VERSION     DAXFZR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.063"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(1..344)
                     /locus_tag="ACFJAS_001587"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="ACFJAS_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            527..1402
                     /locus_tag="ACFJAS_001588"
     CDS             527..1402
                     /locus_tag="ACFJAS_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC3982789.1"
                     /translation="MFYQILALIIWGSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
     gene            1518..2747
                     /locus_tag="ACFJAS_001589"
                     /pseudo
     CDS             1518..2747
                     /locus_tag="ACFJAS_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            2786..3913
                     /gene="pheA"
                     /locus_tag="ACFJAS_001590"
     CDS             2786..3913
                     /gene="pheA"
                     /locus_tag="ACFJAS_001590"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC3982790.1"
                     /translation="MLECTANYRSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKAIGSYPTAVL"
     gene            3950..4693
                     /gene="recO"
                     /locus_tag="ACFJAS_001591"
     CDS             3950..4693
                     /gene="recO"
                     /locus_tag="ACFJAS_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC3982791.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            4746..5474
                     /locus_tag="ACFJAS_001592"
     CDS             4746..5474
                     /locus_tag="ACFJAS_001592"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC3982792.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADNEQIQAARDVGAPVIELHTGAYADAH
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            5524..5901
                     /gene="acpS"
                     /locus_tag="ACFJAS_001593"
     CDS             5524..5901
                     /gene="acpS"
                     /locus_tag="ACFJAS_001593"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC3982793.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            5983..6792
                     /locus_tag="ACFJAS_001594"
     CDS             5983..6792
                     /locus_tag="ACFJAS_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC3982794.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVAVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            6785..7207
                     /locus_tag="ACFJAS_001595"
     CDS             6785..7207
                     /locus_tag="ACFJAS_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982795.1"
                     /translation="MNKNRKLLLAALLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            7256..8398
                     /locus_tag="ACFJAS_001596"
     CDS             7256..8398
                     /locus_tag="ACFJAS_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC3982796.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVAGAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(8444..9262)
                     /locus_tag="ACFJAS_001597"
     CDS             complement(8444..9262)
                     /locus_tag="ACFJAS_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC3982797.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(9344..10594)
                     /locus_tag="ACFJAS_001598"
     CDS             complement(9344..10594)
                     /locus_tag="ACFJAS_001598"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC3982798.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADLAQNTTPRPGRGKNGRKPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(10591..11052)
                     /gene="tsaE"
                     /locus_tag="ACFJAS_001599"
     CDS             complement(10591..11052)
                     /gene="tsaE"
                     /locus_tag="ACFJAS_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC3982799.1"
                     /translation="MSDFSPVPRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            11241..12053
                     /gene="murI"
                     /locus_tag="ACFJAS_001600"
     CDS             11241..12053
                     /gene="murI"
                     /locus_tag="ACFJAS_001600"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC3982800.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT         2566 a         3449 c         3654 g         2735 t
ORIGIN      
        1 gcgttggtgt cgtctttggc gacggcgtcg tcgtcggtgc cggcggcgcc gcgcgtaacc
       61 ggctgtccgc agaaccattt taccgaaccg tcttcacgct tggcccacag ggagagtttt
      121 ttgccttgga tttctttgtt tacgccgtct gatttcattg tggcggtgac gacgccgttt
      181 ttgacttcaa cttgcttaac atatttgcct atgatttttg aagcggatgc cacgccggcc
      241 ttgtcgttgt cttccggcca tttgccgtga ttcaggtaat actcggtgac ggctgatttt
      301 tgaccttcgg ccaaaaggat ggcttcggaa acttgcgcgc gggcgggagt ccggaccttg
      361 gaacaacggc aatattcaaa gattatctga aagtccgaga ttccagattc ccgctttcgc
      421 gggaatgacg aaaagtggcg ggaatgacgg ttcgggcatt ccttcttgtc ctgatttttg
      481 ttaatccgct atacttttgt atgaccatct gactttacca ctcactatgt tttaccaaat
      541 ccttgccctg attatctggg gcagctcgtt tattgccgcc aaatatgtct atggcggcat
      601 cgatcccgca ttgatggtcg gcgtgcgcct gctgattgcc gcgctgcctg cactgcccgc
      661 ctgccgccgt catgtcggca agattccgcg tgaggaatgg aagccgttgc tgattgtgtc
      721 gttcgtcaac tatgtgctga ccctgctgct tcagtttgtc gggttgaaat acacttccgc
      781 cgccagcgca tcggtcattg tcggactcga gccgctgctg atggtgtttg tcggacactt
      841 tttcttcaac gacaaagcgc gtgcctacca ctggatatgc ggcgcggcgg catttgccgg
      901 tgtcgcgctg ctgatggcgg gcggtgcgga agagggcggc gaagtcggct ggttcggctg
      961 cctgctggtg ttgttggcgg gcgcgggctt ttgtgccgct atgcgtccga cgcaaaggct
     1021 gattgcccgc atcggcgcac cggcattcac atctgtttcc attgccgccg catcgttgat
     1081 gtgcctgccg ttttcgcttg ctttggcgca aagttatacc gtggactgga gcgtcgggat
     1141 ggtattgtcg ctgttgtatt tgggtttggg gtgcggctgg tacgcctatt ggctgtggaa
     1201 caaggggatg agccgtgttc ctgccaacgc gtcgggactg ttgatttcgc tcgaacccgt
     1261 cgtcggcgtg ctgttggcgg ttttgatttt gggcgaacat ttatcgcccg tgtccgcctt
     1321 gggcgtgttt gtcgtcatcg ccgccacttt cgccgccggc cggctgtcgc gcagggacgc
     1381 gcaaaacggc aatgccgtct gaagcggttt cgggcggcat atccttcggt tttgccccgc
     1441 cgtccgatat gttagacttg cgcccgtacc gaccgtcgtt tgcgacggtt tctttatttg
     1501 atttgaacgg aaaaaccatg tctccgaccc ttcctccgat gagcggaaaa ctgatggcgg
     1561 ttttgatggc ggtactggtc gcgctgatgc cgttttccac cgatgcctac ctgcccgcga
     1621 ttcccgaaat ggcgcagccg ctgaacgcgg atatccaccg tatcgaatag agtctgagtt
     1681 tgtttatgtt cggcacggcg ttcgggcaag tggccggcgg cgcggtgtcc gacatcaaag
     1741 ggcgcaaacc cgtcgccctg accggtttga ttgtatattg ccttgccgtt gccgccatcg
     1801 tatttgcttc gagtaccgaa cagctcctta acctgcgtgc ggtacaggcg ttcggcgcag
     1861 gcatggctgt agtcatcgtc ggtgcgatgg tgcgcgatta ttattccgga cgcaaagccg
     1921 cgcagatgtt tgcccttatc ggcatcattc tgatggttgt gccgctggcc gcacccatgg
     1981 tcggcgcatt gttgcaggga ttgggcggat ggcgggcgat tttcgttttc ttggcggcgt
     2041 attcgccggt gctgcccggt ttggtacagt atttcctgcc caatcccgcc gtcggcggca
     2101 aaatcggcag ggatgtgttc gggctggtgg cggggcggtt caagcgcgta ttgaaaaccc
     2161 gtgccgcgat gggttatctg ttttttcagg cattcagctt cggttcgatg ttcgcctttc
     2221 tgaccgaatc ttccttcgtg taccggcagc tctaccacgt tacgccgcac cggtacgcat
     2281 gggtgtttgc actcaacatc atcacgatga tgtttttcag ccgcgttacc gcgtggcggc
     2341 ttaaaaccgg cgcgcatccg caaagcatcc tgctgcgggg gattgtcgtc caatttgccg
     2401 ccaacccgtc ccaactcgcc gccgtgctgt ttttcgggtt gcccccgttt tggctgccgg
     2461 tcgcgtgcgt gatgttttcc gtcggtacgc agggcctggt cggtgcggac acgcaggcat
     2521 gctttatgtc ttatttcaaa gaagagggcg gcagcgcgaa cgccgtgtcg ggtgtattcc
     2581 ggtccttaat cggcgcgggg gtgggcatgg cggcaaccgt cctgcacgac ggttcggcaa
     2641 ccgtgatggc ggcaactatg accgcgtccg cctcttgcgg cattgcgctt ttgtggctct
     2701 gctcgcatcg tgcgtggaaa gaaaacgggc aaagcgaata cctttaacgg aaaatgccgt
     2761 ctgaaaccgc ttcagacggc atttgatgtt agaatgcacg gcaaattacc gttcaggcga
     2821 aattatgtcc caaactatcg acgaactcct catcccgcac cgcaacgcca tcgacaccat
     2881 cgatgcggaa atcctgcgcc tgctcaacga acgcgcgcaa catgcccacg ccatcggcga
     2941 gctgaaaggc acgggcgcgg tgtaccgccc cgaacgcgag gttgccgtgt tgcgccgcat
     3001 tcaggatttg aacaaaggcc cgctgcccga cgaatcggta gcacgcctgt ttcgggaagt
     3061 gatgagcgag tgcctcgccg tcgaacgtcc gctgaccatc gcctatctgg ggccgcaggg
     3121 cacgtttacc caacaggcgg caatcaagca tttcggacac gccgcgcata caatggcgtg
     3181 tccgaccata gacgactgct tcaagcaggt cgaaacccgt caggcggatt atctggtcgc
     3241 gcccgtggaa aactcgaccg aaggctcggt cgggcgcaca ttggatttgc ttgccgttac
     3301 cgcgttgcag gcgtgcggcg aagtcgtttt gcgcatccac cacaacctgt tgcgcaaaaa
     3361 caacggcagc accgagggta tcgccaaagt attttcgcac gcgcaggcgt tggcgcagtg
     3421 caacgactgg ttgggcaggc atctgcccaa cgccgaacgg attgccgtgt ccagcaatgc
     3481 cgaagccgca aggctggttg ccgaatcgga cgacggtacg gttgccgcca tcgccggacg
     3541 cacggcggcg gaaatttacg gactcgatat ggttgccgag tgcatcgaag acgaaccgaa
     3601 caacaccacg cgctttctgg tcatgggaca tcacgaaacc ggtgcaagcg gcagcgacaa
     3661 aacctcgctg gccgtttccg cgcccaaccg cgccggcgcg gttgcctcgc tgctgcaacc
     3721 gctgaccgaa tcgggtattt ccatgaccaa gtttgagagc cgtccgagca aatccgtttt
     3781 gtgggaatac ctgttcttca tcgacatcga aggacaccgc cgggacgcgc agattcagac
     3841 ggcattggaa cgcttgggcg aacgcgcttc gtttgtcaaa gccatcggtt cgtatccgac
     3901 tgccgttttg tagtcgcggc agcgttcaga cggcatttcc acaacgatta tgtccgaata
     3961 ccgagtcaac cacgaacccg tttttatgct ggcatcttcg ccttggcgcg aaagcagcct
     4021 gcgggttgaa gcattcagcc gccgttacgg gcgtgtggct ttgctggcgc gcagcgcgcg
     4081 caaacggcag agcgagctgc gcggcgtatt ggttccgttc gtccctgcca gcgtgtcgtg
     4141 gtacggcagt caggaactca aaaccctgca ccgcgccgaa tggatgggcg gatggcggca
     4201 gccgcagggc agggcgttgt tcagcggtct gtatgtgaac gagctggtgt tgaaactgac
     4261 cgcgcgcgaa gacccgatgt ccgaactgta cgacgcgttg gcaaaagtga tggaggcagt
     4321 gtgccgtgaa gccaaccata ttgccgattt gcgccgcttc gaatggaagc tgctcaatgc
     4381 cttgggcgtt gcccccgatt tgcacgccga cggcacgggc ggcgatattt tggcggacaa
     4441 aacctaccgg ctgatgcccg aagaggcggt catgcccgta tgccgggaca caggcgcgct
     4501 ttcgcacgaa gccggcgcaa ttgtcgaagg gcagagcctg atcgatttgc gcgaaggcag
     4561 tttccgcacc gccgaaagcc tgcaacaggc attgaaaatc acacggctgc taatcggcac
     4621 gctgcttccc gaaggcttga aatcgcggca ggtgttggaa cagataaggc agttcgaccg
     4681 caataccgcg tagctgcgcc cgcaccatgc cgtcaaacag gaaaacgaag agaaaaggaa
     4741 acattatgct tctgggtgtc aacatcgacc acatcgccac cgtccgcaac gcgcgcggca
     4801 cgacttatcc cagccccgtg gaggcggcac tggttgccga aacgcacggt gccgacctga
     4861 ttaccatgca cctgcgcgaa gaccgccgcc acatcaaaga cgcggatgta tttgccgtca
     4921 aaaacgccat ccgcacgcgc ctgaaccttg aaatggcgtt gacggaagaa atgctcgaaa
     4981 acgcgcttaa agtgatgccg gaagacgtgt gcatcgtgcc tgaaaaacgg caggaaatca
     5041 cgaccgaagg cggcttggat gtattggcgc aacaagaaaa aattgccggg ttcaccaaaa
     5101 tcctgaccga cgcgggcatc cgagtctcgc tcttcatcga tgccgacaac gagcaaatcc
     5161 aagcagcccg tgatgtcggc gcacccgtta tcgagctgca caccggcgca tatgccgatg
     5221 cacacagcca cgccgaacaa ctcaagcaat tcgagcgtct gcaaaacggc gcgcatttcg
     5281 ccggcgattt gggcttggtc gtcaacgccg gacacggact gaccatacac aacgttaccc
     5341 ccgtcgccca aatcctcgcc atccgcgagc tgaacatcgg gcattcgctg atttcgcagg
     5401 cacttttcct cggattgccc gaggccgtgc gccagatgaa ggaggcgatg ttcagggcaa
     5461 ggctgctgcc gtaagggcag gcaaaccgtt tcagacggca tttcacgaca aaggagcgca
     5521 aatatgattt acggcatcgg tacagacatc gtttccctca agcgcatcat ccgcttaaac
     5581 aaaaaattcg gacaggcgtt tgcagggcgc atactcactc ccgaagagct gcttgaattt
     5641 ccgcaggcgg gcaaacccgt caactacctt gccaaacgct ttgccgccaa agaagccttc
     5701 gccaaagccg tcggcacggg catacgaggc gcggtttcct tccgcaacat cggcatcggg
     5761 cacgacgcat tgggcaagcc cgaatttttc tacggccccg ccctgtccga atggctggaa
     5821 gaacaaggca tcagccgcgt cagcctgagt atggcggacg aaggcgatac cgtgttggcg
     5881 tttgccgttg ccgaaaaata atgccgtctg aaattcggca aacccgttga cggcattgcc
     5941 cttccctcat ttgcattccg accgaccaac cgcatacccg ccatgatcca agatacccgc
     6001 ccccttatcc gcgtcgttgc cggcatcctg ctcgattcag acggcaatta cctgctcggc
     6061 tcgcgccccg aaggcaagcc ctatgccgga tattgggaat ttgccggcgg caaggtcgag
     6121 gcgggcgaaa ccgacttcca agccctgcaa cgcgagtttg gagaagaact cggcatccgc
     6181 atcctcgccg ccacgccttg gctgaccaaa atccattcct acgaacacgc ccgcgtctgc
     6241 ctgaaattct tatgggtaaa ccccgatcaa tgggagggcg gaccgcaatc gcgcgaaggg
     6301 caggaatggt cttggcagaa ggcgggtgat tttaccgttg cccccatgct gcctgccaac
     6361 ggtgcgcttt tgcgttcgct gtccgtcccg cgccgtttgt acggcagcct gaaaacgggt
     6421 ttgtacggcg aaaacagtat gggcgcgtac cgcgtcctgc ctttgggttc ggcaggggga
     6481 agcggtgcga acgttttgat ggaggcggca caatggcagg acaggtccga acacgccggc
     6541 agcgtgtgga tgatggtgca gacccgcgaa caatggcggc aggcgcagga aaagggcgcg
     6601 gatgtggccg tgtggcgcgt gtgcgatgat gttcaggcac aagaggcggc agaagccctg
     6661 cggcagggcg tatccgtgcc gctcgtactt gcagcaaacg gacagacggt tgcacgttat
     6721 ggaaaactat ggctcggatt gggggcgcac gcggtggtaa gggatgaaac aatagggaag
     6781 aatcatgaat aaaaaccgca aattactgct tgccgcactg ctgctgactg cctttgccgc
     6841 cttcaagctc gttttgttgc aatggtggca ggcgcagcag ccgcaagccg tggcggcgca
     6901 atgcgatttg accgagggtt gcacgctgcc ggacggaagc cgtgtccgcg ccgccgccgt
     6961 ttcaaccaaa aaaccgtttg atatttatat cgaacacgcg cccgccggca cggaacaggt
     7021 cagcatcagc ttcagtatga aaaatatgga tatgggtttc aaccgctata tgttcgagcg
     7081 gcaaccgtcg gggacttggc aggcagcacg catccgcctg cccgtctgtg tcgaaggcag
     7141 gcgcgatttt acggcggaca ttacaatcgg cagccggaca tttcagacgg catttaccgc
     7201 cgaataaacc tttcaatccg ccattgccgg aacatccgtc cggaaaggac acgctatgaa
     7261 tactttatat acacttttcg ccacctgccc gcgcggcttg gaaaccgttt tatctcaaga
     7321 actcggaagc ctcggctgta ccgatgtcca agcgtttgac ggcggcgttt cctgccgggg
     7381 cggattggaa caggcctacg ccgccaacct gcactcgcgt accgccggcc gcatcctgct
     7441 gcgcctgacc aaaggcacat accgcaccga gcgcgacatc tacaaacttg ccaaaaacat
     7501 caactggttc aactggttta ccttgcagca gacgttcaaa gtcaaaatcg aggcaaaacg
     7561 cgccaacgtc aaaagcctcc aatttgtcgg cctgaccgtc aaagatgccg tctgcgacgc
     7621 tttccgcgac atttacgacg cacgtccgag cgtggacaaa gccgcgcccg atgtccgcat
     7681 ccacgccttt ttggacgaac gcaatgtcga aatcttcatc gacacttcgg gcgaagccct
     7741 gttcaaacgc ggctaccgcc tggataccgg cgaagccccg ctgcgcgaaa accttgccgc
     7801 cgggctgctg ctttcggcag gctacgacgg cacgcagccg tttcaagacc cgttttgcgg
     7861 cagcggcacg attgctatcg aagccgcttg gattgccgcc ggccgcgcgc cgggtatgat
     7921 gcgccgcttc ggttttgaaa aactgcaaaa tttcgataaa acgctgtggg cggatttgcg
     7981 ccgccgcgcc gaagcgcaaa cccgccccgt ccgcgccccg attgcaggca gcgacaacgg
     8041 ccgccgcatc gttcagacgg cattggacaa cgcacacaat gccggagcgg acgacatcgt
     8101 ttccttcagc gttgccggcg cgcagtccgt ccgtccgaac ggcgaaaacg gcatcatggt
     8161 gtccaatccg ccctacggcg tgcgccttga ggaagtccgc gctttgcagg cactttatcc
     8221 gcagttgggg acgtggttga aaaaacacta tgccggctgg cgggtcggca tgtttaccgg
     8281 cgacagggaa atgcccaaat tcatgcgcct gctgcccaag cggaaaatcc cgctttataa
     8341 cggcaacctc gactgccgcc tgttcctgat cgatatggtg cagggatcga accgttgagg
     8401 aaagtgtaca aaaaagccgt ctgaaaaatg ttcagacggc tttttaaatg gagaaatttc
     8461 ggaaaatatc cacatcaggc tggggaccgt acttatttac ggtattttcg tatgccgaaa
     8521 taaaatattt cttgaataaa ccgtaatcgc cggtttcgta ataggctacg acagcctcgg
     8581 cataggcggg ataactgtcg ggggagaata cgcaaggaaa aaaaccggag cgcatcagcg
     8641 acaaggtcat acagtttctg gcagtacgtt tattgcagtc cttaaaatat cggagatacg
     8701 ccaaattatt atggagataa acggcgcggt cgaaaggatt ttctattttg ggggcttctt
     8761 gaagcaacca tttcaattcc gtatccaaac tttgcgggtt ggataaaggg gtgtagtctg
     8821 tcccgcttat agtaacgctg tcgcggcgga ctacgccgcc ggagcctttt tccagtaagt
     8881 tttccgaaat caggctgtgg gtggttttta agaaatccaa ccagtcaaac ggcttggggc
     8941 tgtccaaacc ggacagcagg tgccggtaac tttccctcag gttgattaac attacggcat
     9001 ccgaatataa cttcccgcct gcggtttgcc cgagtttaag caatgcctgc gtatcgtatt
     9061 gattataagt gtttccttcc aatttggcag aactgtacac aaaatcgata ccgatttctt
     9121 caatattacg cacggagtcc gttaaaacag ataaaggaag ccgatcggac aattcggtca
     9181 ggtattcttg ttcgccggct gtaaaaatgg ggcggatatt taaaaaatcc cgttggtatc
     9241 tcaatttatc catacctttc atcataaaaa cgcggtttgg ggcggaagta ggtttatgcc
     9301 tgccttcatc cgacaactat attttgaaac accgcctttt caatcaaccc cgcttcaata
     9361 cggatgtatt gatgtagcgt tggacacccg aggcaatgga ttgggcgcac tgccgacgga
     9421 aggattcgct gcccagcagc ttctcttcgg caggattgga caggaaggcg gtttcgacca
     9481 ggatagacgg catatcgggt gcgcgcaata cggcgaaatt ggcttcgtcc accctgcctt
     9541 tgtgcagatg gttgagcctg cccaattctt caagcaccag tttgccgagt ttgcggctgt
     9601 cgcgcagtgt ggcggtttgc gtcatgtcga gcagggcggt atcgacgttg cggttgccgc
     9661 tggtcggtac gccgccgacc gcgtcggcat tattttgggt ttgttccaag aatttggcgg
     9721 cagagctggt cgcgcctttg gtgttcagca tataaacccc cgttccgcgc gcggaggggc
     9781 tggtgaaagc gtcggcgtgg atggagacga atacgtccgc ccgccgtgcg cgccctttgg
     9841 cgacacgcac gcccaatggg atgaacacgt cttcgttgcg cgtcataaag acgttgtaac
     9901 ccaaggcttc caattgtttt ttggtttcgc gggcgatgga gaggacgacg tgtttttcct
     9961 gcaaaccgcc cgggctgacg gcgccggggt cttcaccgcc gtgtcccgga tcgagcatga
    10021 tgacgggttt gcgcccgttt ttgccccgcc cgggtcgggg cgtggtgttt tgggcgaggt
    10081 cggcttccgg agagccgcgc agggttttgt tcaggctgcc gttgagcagc gccatcatcg
    10141 gatcgtcggc atccatcccg tgcggataga ggtcgacgac gaggcggtcc ttaaagccgc
    10201 cgacgggcgg aagcgcgaag acttgtgcgt gggtgggctg tttcaggtcg atgacgaggc
    10261 ggacggtggt cggcgtgttc tgacccgcgc gtatgctgcg gataaagggg tcgtccgcca
    10321 taactttttg ggaaagcccg tgcaatacgg tattgatgtt tgcgttttgt atatcgacga
    10381 ccaacctgcc cgggttgtcg agcgcgaagt gctggtattt gagcgcggcg gtgctttcca
    10441 gcgtcaggcg ggtgtaggtg tgcgacggcc atatccgtgc ggcggtaaac tgcggggcgc
    10501 gtaccgtttt ggcaacggcg gatgcgatgg ggctcagggc gaacagtgtg ccggcggtgc
    10561 ggcggatgat ttgtcttcgt gtcagtttgg tcatagcggc aggctttcgc gtcctcgttc
    10621 ggtatgggcg gtcagcaggc attttctgcc gccgccgccg tgtgtcaatg ttgcggtgat
    10681 gtcggcaggc ggggtaaatt cgcctccctg ttgcggccat tcgatcaggc agacgctgtt
    10741 tgcggcaaac agctcgtcaa gccccgcgtc ttcccattct tcggggaacg agaagcggta
    10801 gaggtcgaaa tggtgcaggg cgaagcgttc cagcggataa gattcgacga tggcgtaggt
    10861 cgggcttttg actgcgccct ggtgacccaa tccgcgcagg atgccgcgcg tcagcgtggt
    10921 tttgcccgca cccaaatccc cttcgaggta aatgaccagc ggtgcgttta aacgggaaga
    10981 ccacgccgcg cccaaatcga gtgtggcggc ttcgtcggca aggaatcggg gaacggggga
    11041 aaaatcagac ataggggctt tacaactttt atgtggcgga tcggaggggc aggcggtcgg
    11101 caaaccgtgc gcccggcggc ggcattgccg tacattataa cagccaaata cgcatcctgc
    11161 tttcagacgg catggctgtc aagaaaaagc ggcgcgtgta caatatgcgg attgtatgtt
    11221 taggacggat tggaaaaagg atggaaaata tcggcaggca gcggcccatc ggcgtttttg
    11281 actcgggaat cggcggtttg accaatgtgc gcgcgctgat ggaacggctg ccgatggaga
    11341 acatcattta tttcggcgac acggcgcgcg tgccttacgg gacgaaatcc aaggcgacca
    11401 tcgaaaattt ctcgatgcag attgtcgatt ttttattggg acacgatgtc aaggcgatgg
    11461 ttatcgcgtg caacacgatt gcggcggttg cggggcggaa aatccgtcaa aagacgggca
    11521 atatgcccgt tttggacgtg atttccgccg gcgccaaagc cgcgctggca acgacgcgca
    11581 acaataaaat cggcatcatc gccaccaata cgacagtcaa cagcaatgct tatgcgcgcg
    11641 ccatccacag ggacaacccc gacacgctcg tccgcacgca ggccgcgccg ctgctcgtcc
    11701 ctttggtgga ggaaggttgg ttggaacacg aagttacccg cctgaccgta tgcgaatacc
    11761 tcaaaccatt gcttgcagac ggcatcgata cgctggtgtt gggctgcacg cactttccct
    11821 tgctcaagcc cttaatcggc agggaggcgc acaatgtcgc gctggtcgat tccgcgatta
    11881 cgacggccga agaaaccgca cgcgtccttg cacaggaagg attgctcgat accggcaaca
    11941 acaatcccga ctaccgtttt tacgtcagcg atattccttt gaaattcaga accatcggcg
    12001 agcgttttct gggcaggacg atggagcaga ttgaaatggt gtctttgggt taaaacgata
    12061 acggaaagct gcccgagatt acagagacct aaaatcccgt cattcccgcg aaagtgggaa
    12121 tctagacctg tcggtgcgga aacttgtcgg ataaaacggt ttctttagat tttacgttct
    12181 agattcccgc tttcgcggga atgacgggat gcaggtttct gtgcggatgt attcggattc
    12241 cctcctgcgc gggaatgacg ggatttttaa atgtccgaat gccgcaaatg ccgcctgaaa
    12301 ggcttcagac ggcatttttt acacaattcc caccgtttcc catcatcccc gacaataccg
    12361 taatcttgaa atccgtcatt cccgcgcagg cgggaatccg gacc
//
LOCUS       DAXFZR010000064        12360 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000064 DAXFZR010000000
VERSION     DAXFZR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12360
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.064"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            558..1862
                     /locus_tag="ACFJAS_001601"
     CDS             558..1862
                     /locus_tag="ACFJAS_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC3982801.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(1956..3251)
                     /locus_tag="ACFJAS_001602"
     CDS             complement(1956..3251)
                     /locus_tag="ACFJAS_001602"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC3982802.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLCQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(3378..4265)
                     /locus_tag="ACFJAS_001603"
     CDS             complement(3378..4265)
                     /locus_tag="ACFJAS_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC3982803.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(4265..5230)
                     /locus_tag="ACFJAS_001604"
     CDS             complement(4265..5230)
                     /locus_tag="ACFJAS_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC3982804.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISSA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(5246..6370)
                     /gene="potA"
                     /locus_tag="ACFJAS_001605"
     CDS             complement(5246..6370)
                     /gene="potA"
                     /locus_tag="ACFJAS_001605"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC3982805.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLNIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(6648..6917)
                     /gene="rpsO"
                     /locus_tag="ACFJAS_001606"
     CDS             complement(6648..6917)
                     /gene="rpsO"
                     /locus_tag="ACFJAS_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC3982806.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(6998..7132)
                     /locus_tag="ACFJAS_001607"
     CDS             complement(6998..7132)
                     /locus_tag="ACFJAS_001607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982807.1"
                     /translation="MWSDVKCRLKPEDVSVFRRHFADIAKYRAEQQKLCDNALFLFQE
                     "
     gene            complement(7122..8057)
                     /gene="secF"
                     /locus_tag="ACFJAS_001608"
     CDS             complement(7122..8057)
                     /gene="secF"
                     /locus_tag="ACFJAS_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC3982808.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(8061..9914)
                     /gene="secD"
                     /locus_tag="ACFJAS_001609"
     CDS             complement(8061..9914)
                     /gene="secD"
                     /locus_tag="ACFJAS_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC3982809.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(10125..10391)
                     /gene="yajC"
                     /locus_tag="ACFJAS_001610"
     CDS             complement(10125..10391)
                     /gene="yajC"
                     /locus_tag="ACFJAS_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC3982810.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            10522..11640
                     /locus_tag="ACFJAS_001611"
     CDS             10522..11640
                     /locus_tag="ACFJAS_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC3982811.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPEHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
     gene            11784..12233
                     /locus_tag="ACFJAS_001612"
     CDS             11784..12233
                     /locus_tag="ACFJAS_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3982812.1"
                     /translation="MNTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYLNRQKTR
                     YLRDRRGRPAHPLSSMFKAVLPGQWHSLSDPELEHSLITRIGFNLFCRFDGPGIPGCS
                     TLCRYRKFRYARAAYFGLLKVGAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT         3046 a         3298 c         3152 g         2864 t
ORIGIN      
        1 cattttcatt cgcacggccg gcgcagctcc cccccccccc gctaacggcg caaccgccgg
       61 cagtgtccgt gtccgcctgc cgaaggcgcg gcctttgcaa aaccggctcg aacgcatccc
      121 taccggcaat gaaaaccgat gcgggaactt gattcaaggt tgcgccgaac cgcgcccatt
      181 tttgtataaa ttttattttt tatttccaat tagaaatttt taatatatta atcaataaat
      241 taattttata aaataaaaat attgtcaaca atcttttgac ttatcgccca aacctctgta
      301 tattttccta cagtaaattg ttgacaatcc atacgcccgc atatgcgccg cctaaggata
      361 aatcctcccg ccggacaacg ggggcaaggg ataggatgcg atatttccat attcaaacaa
      421 gggatttgtt tcacgcacag ggcggcacat cggcaaaatc cccgcgccgg tccggcaggg
      481 cttgcgccca tcccggacaa gccccgaccc cgcctttccg aaagacgggc ccaaccatta
      541 aggaaacttt aatcaaaatg aaaaaacaca tatgggcggc atctttgctg ccggcatccc
      601 tatcggcaga acctttaaac tggtggaaac cttattccgc cgtcaattcg ggcgataccg
      661 cctgggtgat gactgcggct gccttggtac tgttgatgac gcttcccggg ctggctttat
      721 tctacggcgg tatggtgcgg aaaaaaaacc tgctctcgac gatgatgcac agcttttcca
      781 tcgcgacatt ggtgggcatc ctttgggtcg ccgtcggcta ttctttggcg ttcacgccgg
      841 gaaatgcctt tatcggcggt ttggggcgcg tatttttaag cgggatgcag atagacgctg
      901 ccgcacggat gctgaccgtg tcgcccaatg cgccgactgt tcccgaaccg gtatttatgt
      961 tttttcagat gacgtttgcc attatttcga ccgccattat taccggcgcg tttgccgaac
     1021 gaatgaaata ttcggcaatg atgctgtttt cgggcatatg gtttttattg gtttatgtgc
     1081 cgggcgcgca ttgggtgtgg ggcggcggct ttatgagcaa gggcggcgta ttggattatg
     1141 ccggcggtac ggtggtgcac atcaatgccg gtatcgcggg actcgtcgcc gccttggttt
     1201 tgggcaggcg cataggctac gggcgcgagg cgatgcctcc gcacaatatg gcgatgacgc
     1261 tgattggcgc ggcaatgttg tggttcggct ggttcggctt taacgctggt tcggcgcttg
     1321 cggcagacgc ggcggcgggg atggcgatgg cggtaacgca ggtgtcggcc gtattcggcg
     1381 cggcaggctg gcttgcctgc gagaaaatag cgggacacaa accttctgct ttggggctgg
     1441 cttccggcgc agtttccggg ctggtcggca tcacccctgc cgccggcttt accggtccgt
     1501 cgggcgcggc cgccatcggt atattgactg ccgccgcgtg ctttgtgtcc gtcaccgtcg
     1561 tcaaacacaa attgcgttac gatgattctt tggacgcttt cggcatacac ggattcggcg
     1621 ggctggtagg cggaatattg accggcatct ttttcgacaa ccgcattttc ggtggggatg
     1681 cggcagtttg gcagcagttg tggatacagg taaaagacgg gttcattatg gcggcataca
     1741 gcgggctgat gagttgggcg attttgaagg ctgtggggaa aatctgcggc ggtctgcgcg
     1801 tcggcaagga tgtcgaacgc gaaggtttgg atctgaatat ccacggcgaa cacgtggaat
     1861 aaggacggct atgccgtctg aagcctgaaa atcgggtttc agacggcatt tttcacgttt
     1921 gccgccgatg gggataaaca tactgcctgc gacgcttaac ggctgtcttt ccactgataa
     1981 tatttcgagc cgaggaaaga accgagtttg cgcaaaaacg gttgcaggat aatattcggc
     2041 tggcacaact gttcaaacgg tttcagacgg cattcgtccc ctaaaattgc ttcggcaacc
     2101 gccaggcctg caatgcccgt tatcgccatc ccgtgcccgg aataaccttg cgtataaaaa
     2161 acattcgagg tcaaacgtcc gaaatgcggg acaaggttgg cggtaatatc gcactccccg
     2221 ccccacgaat attcgattct aacatcggca agctgcggaa aaactttaag catatcttgg
     2281 cggacaagct cggtcatacg cgcaggattg tcgataaatt cgttatcctt accgccgaaa
     2341 agcagcctgc cgtccgcgct gaggcggtaa taatccaaaa tatggcggtt gtcgcatact
     2401 gccatattgt tgcggataag cccttttgtg cgcgcgccca agggttcggt ggcaataata
     2461 aaggtgctga cggcaatcgc cttgcgttcc aaaggccgga atatcgggtt caaaccgaca
     2521 taagtattga cggcatagac cacattttta cactcgacgc tgccttcggg cgtgtaaacc
     2581 agccaaccgt tttgatacgg ttcgatgcgc gtcatcgggg attgctcgaa aatctgcgcg
     2641 ccggcttcgg cagcggcgct ggcaacaccc aacgtgtaat tgagcggatg aagatgcccg
     2701 gacaagggat cgaactgtgc gccttggtac atatcgctgt caagctgctg tttcaactcg
     2761 gctttatccc aaagttgata atgactcgca ccgtaatgcc gttgggcgtg ttcatgccac
     2821 tgctgcaact cttcccaatg ctgcggacgg acggcaaccg tggcataacc gcgctgccaa
     2881 tcgcaatcga tggcatgttt gcggacgcgt tcgtccacca gttcgaccgc ctgcaaagac
     2941 tgttgccaaa accattgcgc ctgctccaag ccgacctgtt tttcaatttc ccccataccg
     3001 caggcgtagt cgctgataac ctgcccgcca ctcctgccgg acgcgccgaa gccgatacgt
     3061 gcggcttcca aaacgacggc ttcatgtccg tgttccgcca gcggcaatgc ggtacacaaa
     3121 ccgctcaaac cgccgccgat aatgcaggtt tcggctttca gacggcattg gagtttcgga
     3181 taaacagtat gcggattaac cgaactaaaa taataagaag gcagatattc ttgaaaatca
     3241 gggcgaatca ttgtgtttgc tttatcgggt atattttcgg acggaatgat acagactgtc
     3301 gggccatatc gtccaaacag aaaatcggtt gaaaaaaaac aggctgaccc agtcatgcgg
     3361 tcagcctgcc ttattaatta atttgctttc tcggcagcca atttttcttg gcggtaggct
     3421 tctgccgctt ctcggttgcg cttggttgcc tgcctcatca tccaataatt gacgatgatg
     3481 accaatgttc cgatgatgcc gatcaggatg gttgccaaga cattcatctg aggatcgaga
     3541 cccaacttga tcttggagaa aataacctgc ggcaatgtgg atgaaccggg gccggagagg
     3601 aatgaggtaa ttaccaaatc atccaaagac aaggtaatgc cgagtaggaa gcctgaagca
     3661 atggcagggg caatcaaagg caaagtgatg acaaaaaaga ttttcagcgg acgcgcgccc
     3721 aagtccattg cggcttcttc gagcgactgg tcaagctcga ccagccgcga acggatgaca
     3781 acggtaatgt atgccatgca cagcgtcgta tgtccgagga agatggtgaa aaagccgcga
     3841 tcgaagtaga gatgttgcaa ccattcgctg ccctgcaaaa atatctgcac ctgaataatc
     3901 agcagcagca tagacagacc ggtaatcacg tcgggcatga ccatgggcgc ggaaatcata
     3961 ccggcaaaca gcgtgctgcc gcgaaagcgt ttgatacgcg ccatcgcata gcccgccagc
     4021 gtgcccaaaa cgacggcggc aagcgaagac acaacggcaa tccgcagcga cagccaagcg
     4081 gcttccaaaa tggtgtcgtt ttccagcaat gcgccgtacc acttggtcga aaagccgccc
     4141 caaacggtaa caagtttgga ttcgttgaac gaatagatga ccaaaacaac cagcgggata
     4201 tacagaaacg ccagcgatag tgccaacatc agtttcaaga accaagataa tttggatttc
     4261 tgcattattt ggctccttct tccaattcgc ggttttcata gtgctggaac agggcaatcg
     4321 gtacaaccag tagcgcgacc atcacgacgg cgacggcaga agccaacggc cagttgtttt
     4381 gatcaaagaa tgcctgccac aatactttac cgatcatcag gttttccgaa ccgccgacca
     4441 gctcgggaat gacgaactca ccgacagcgg ggacgaaaac cagcatcgaa cctgcaataa
     4501 tgccggtttt cgacaaaggc agggtaatcg tcaagaacga tttgaccggt cccgcgccca
     4561 agtcggaagc cgcttcgagc aggcggctgt ccagtttcac cagctgcgtg taaagcggca
     4621 gaatcataaa cggcagatag gcataaacca ttaccaaatt taacgaaaac gcattataga
     4681 acaaatctaa tggttctctg ataataccca tcttaatcaa taagttgttt atgatgccgt
     4741 tatgcccgag caaacccatc cacgcataga cacgcagcag gaacgatgtc caaaaaggta
     4801 acatgatggc aagcagcaaa ccattgcgga cggaaggatt ggcacgggaa atcgcatagg
     4861 cggtcgggta accgaccaac aggcaaatca ccgtcgtcgt cagcgcagtc ttaattgaag
     4921 accaataagt catcagatag atattactgt tttcactgtc gccgaagggg ttaagggtat
     4981 gccaaaaatt ttggaaaata tcggcatagt tttgatagct gacggcgatg ttcaggcgac
     5041 ccaagtcctc atctatcgtc gttaaaggag taaacggtgg aatggcgatt tcttgttcgg
     5101 cagagctgat tttcagcacg atggcgaacg gaatcagaaa cagcaccaaa agccaaatat
     5161 acggtacggc aattaccgca cgctgccccg gacggcggaa cagtttgttt ttcagtttat
     5221 taaggttcat ctcactcccc ttaaatcaac ggaacaacgg agtcggttgg ttttccggcc
     5281 agctgatata gacggtttca tcccaagtcg gcggcgtgat gttacgcaca taccagtaag
     5341 gtgcaggaac ttggcttttg acgacgcgcc cgttggcgag tttgatatgg tatatggcga
     5401 agctgcccaa ataggcaatt tcttttaccg tgcctttcgc ccagttgtag tcgcccaaat
     5461 attcgggttt ttctttatat aaatcaatat cctctggtcg aatactaacc caaagatcct
     5521 gctcgctcgg gccacccaaa ccatggtcga tacggacgtg gttttccaaa ccttcgcatt
     5581 cgataacggc atagtcggca tgattttcaa tcaccacacc gtcaaagatg ttggtttcgc
     5641 cgataaactc ggcagtgaag cggctgttgg gatagtcgta cacgtcgctg ggcgtgccga
     5701 cctgctgcaa ctgaccgtcg gacataatgg cgatgcgggt cgccatcgtc atcgcctctt
     5761 cttggtcgtg cgtaaccata atgcaggtta cgccgacttg ttccagcgta ttgaccaact
     5821 cgagctgggt ttgctggcgc aatttcttat ccaacgcccc caaaggttcg tcaagcaaca
     5881 ggattttcgg acgttttgcc aaactgcgcg ccaaggcgat acgttgctgc tgaccaccgg
     5941 acaattggtg cggtttgcgt ttggcaaatt tggtcatctg aaccagacgc agcatttctt
     6001 cgacgcgcgc atcgatttcg cctttaggca ttttgtcctg tttcagaccg aaggcaatgt
     6061 tttgctccac cgtcatatgc ggaaaaagcg cgtaactttg gaacatcata ttgatggggc
     6121 ggtcataggg tgcaagtttg gtaatatctt gaccatcaag gatgattttt ccctgattgg
     6181 gactttccat acccgccagc atacgcagca gcgtagattt tccgctgccg gaactgccca
     6241 aaagggcgaa gatttcgttt tgataaatgt tcaagtcgat gttatcgaca gcgtaattgt
     6301 caccaaactt tttcaccaaa ccttggattt gcagataagg tttggctgaa gacgctgtgg
     6361 ttgcggtcat aatggcaata ctccaataaa aagacgagta ccggcaaaac ggattttcga
     6421 atgggtgata aaaagctgtt tgattgctgg cgggagtcgc aacgtctgat gccgtctgaa
     6481 actcttgtga agcgcacggg cagcatgaaa taaaaagggg ttctaaagaa ccccgcatta
     6541 tattagttta taaggttttt agggtaataa atatgggttt gagcttgtcg gaaagcaaca
     6601 gaaaagaaaa caccgcccat ttttctgggc ggtgtcggaa agcgcaatta tttacgcaga
     6661 cccaagcggg taatcaacgc gcgatacgta tcgggctggg tacggcgcaa ataggccaac
     6721 aggcggcggc gttggctgac cattttcaac aggccgcgac ggctgtggtg gtctttgggg
     6781 ttggctttga agtggggggt cagatcgttg atgcggaaag tcaacagggc aacttgtact
     6841 tcagaagagc cggtgtcgcc ttctttacgt tggaaatctt taacgatttg tgctttttgt
     6901 tctacggtca gtgccataat ggaaaaactc cgaaaaataa aaagaacccc aaatgggatt
     6961 cggacaagtt tgccaagcct cgacaacggc aaactcccta ttcctgaaac aggaacagcg
     7021 cattatcaca caatttttgc tgttctgcac gatattttgc gatgtcggcg aaatgccgtc
     7081 tgaaaacgga gacatcttcc ggtttcagac ggcatttgac atcagaccac aatttcttcc
     7141 ttctgtttcg cttccttggc gatattgtcg cggctcaaac cgaacatcag caagagcggg
     7201 ctggcaacca atacggaaga gtagatgccg aacacgatac cgatggtcag tgccatagaa
     7261 aagccgtgca aggctgcacc gccgaacacc agcatagaaa caaccatcgc ctcggtcgaa
     7321 ccgtgggtaa tgatggtgcg gctcatcgtt gcggtaatcg cgttgtcaat gacttccggc
     7381 acggtatgtc cgcgcatcgc cggcttgcgg aaattttcac ggatacggtc gaagacgacg
     7441 acggattcgt tcacagaata gcccaatacg gcaaggatac ctgccaagac ggtcagcgaa
     7501 aattcccatt ggaagaaggc aaagcagccg agaataatca cgatgtcgtg catattggcg
     7561 ataatggcag atacggcaaa acgccattcg aaacgcatcg acaggtaaat aatgatgccg
     7621 ataacgacaa aacctaaagc catcaatcca ttatttacca attcctcacc gacttgcggg
     7681 ccgataaatt cgacttggcg caaagtaacg tcgggactgt cttttttcag caaatccata
     7741 acctgattgg acaactgtgc ggaagtaaca ccttctttgt tcggcaggcg gatcatgatg
     7801 tgtttgttcg tacccaatgc ctgaacctgt acatcaccca ttttcagcgt atcgaggcgt
     7861 tcgcgcatct tattgacatc cgcaccctgc tgatattgga cttccattac cgtaccgccg
     7921 gtaaattcga cagagaaatt caggcctctg gcaaccaaaa agaatacggc ggcaataaag
     7981 gtaaccaacg aaatgaaggt tgtcagtttg ccgtagctca taaacggaat atcgcgtttg
     8041 attttaaaga gttccatagc ttactcctta cctgccgcag tttcggcttc aggtttccat
     8101 accgcaccga tggaaatatt ctgcaatttg cggcggcggc cgtaccacag attgaccaac
     8161 gcacggaata cgacgacgga cgaatacatc gaagtcagaa tacccaagca gtgtacgacg
     8221 gcaaaacctc ttaccggtcc ggaaccgaat accaaaagcg cgataccggc aatcagcgaa
     8281 gtcaggttcg aatcaacaat ggtcgcccat gcgtgttgga aaccgagatt gattgcctgc
     8341 tgcggcggca cgccggcacg caattcctct cggatacgtt cgttaatcaa gacgttggag
     8401 tcgattgcca tacccaaagt caacgccagc gcggccatac ccggtaacgt caacgttgcc
     8461 tgcatggcag acaaaatacc gattaggaac agtatgttgg cactcaatgc aatagtagaa
     8521 aagaaaccca tcaggcggta ataaaccacc atgaatgcag caacgatggc aaaaccccat
     8581 aaagtcgaat ggaagccttt ttcgatgttc tccttaccca aagacggacc gatggtacgt
     8641 tcttcgacaa tctgcatcgg tgcggcaaga gaaccggcac gcaacagcaa agacgtatcg
     8701 ttggcttcgg ctgtcgtcat gcttccggaa atttccacgc gtccgccggt aatggcagta
     8761 cggataaccg gtgcggttac aacctcggat tttccttggt cgatcaaaac catcgccatg
     8821 cgtttgccga catttgcggc agtcagttcg ccgaaaatgc tgccgcccgc gctgtccaag
     8881 ctcagactga cggcaggtgc gcccatttgg tcgaaactcg gttgcgcatc gttgatgttg
     8941 tcgccggtca gctcgacctg tttgctgatc agcagagttt cgggatgctc cccgccgctt
     9001 gaaagcagct cataaccgct cggcacgttg ccttccaatg cctcgcgcaa cttggcagga
     9061 tcgtcctcca ccatgcggac ttccaaagtc gcggtacggc cgatgatgtc ttttgccttg
     9121 gcagtatctt gcacgccggg aagctgtacg acaatgcggt ccaaacctga ctgttggata
     9181 atcggctctg ccacacccaa ttcgtttaca cggttatgca gggtagtgat gttttgcttc
     9241 accgcatctg atcgtacttt attcaccgcc tcttccgaaa gcgccaatac aatattgctg
     9301 ccttctgaat ttaacgttgc ctcaggaaac agcttgagta actgaggcag tgccttttgc
     9361 acatcgccgg catcctgcaa agggacggtc aggctgtttt cagcctgacg caccgtgccg
     9421 ctgcggattt tttcacggcg cagttcgcgg cggatgtcgc ccgaataacg ctcaaacgtt
     9481 ttctgcatcg cggctttcat atcgacctgc atggtgaaat gcacgccgcc gcgcaggtcc
     9541 aaacccaaaa acatcggatt ggctttgatt ttcgccatcc attcggggct gtccgccaac
     9601 aggttgagcg cggtaatata cccttcgccc aaagtgtttt cgataacgtc gcgcgcttta
     9661 agctgcgttt ctgtgtcttt gaaacgcact ttcagtgaat tatccacaac aaacatcccg
     9721 tcggtctgaa tgcccgcgtt tttcagcgca gcatccactt tggattgagt ctgttcgttg
     9781 atgatgatgg cttgtcggtt ggtcgatacc tgcacggcgg gcgtttcgcc gaataggttg
     9841 ggcagcgaat acactgcggc aaccgcaatc gtgaacacaa tcagcagata tttccataaa
     9901 ggataacggt tcatcattgt tccttaatgg ttggaacccc accctttcgg tggtgtcgga
     9961 atcgggctat ttcagaagag gcaaaacccc tccctgccag gcaagaccga aaggcagcat
    10021 cttgaatatg ccgccctgcg tgtcggaaca tggtcaagcc ttcggttgga attcaaaaca
    10081 aagtgccgca ttcgggcttt cccgatgcgg cttgtcggca caaatcaatc gacttttgcg
    10141 gcaatcgcat tgcgttccac ttcgacctcg atttttgtac cctgtccgat atccacggta
    10201 aaaaactgtt cgccgactct ggttacctta cccttgaaac ctgccgccaa gaccactttg
    10261 tcgccggctt tcaaggcggc aagcattgcc tgatgcgctt tgaatttctt ttgttggggg
    10321 cgcatgatca ggaagtagaa caccaccata atcaacacca aaggagcaaa ttgtgcaaca
    10381 gcttgattca taatttatcc gttctttcta aatatgattg aaaatcgagc ggggtatata
    10441 atagcataag accgtaaaca atatatcggg tttgccgccc atacccaacc gtatgccgtc
    10501 tgcaccccct ttcccgaacc catgccgctg acccgcctgt tcctgaaact ctacgccctg
    10561 ttgcgccttt ttttgggcaa aaacgcccag accgcctgga ttttccaccc cgcctgcacc
    10621 gggcacgacc ccggggcaaa ccatcccgat tcgcccgacc gcatcctctg catcgagcag
    10681 gcattgcgcc gcgccggtat ttggcagcac ctccaaaccg tagaggcgga agaaatcagc
    10741 gatacgcgcc ttgcacttgt ccgctcgagc aaatatctga accgtttgga atcttgccta
    10801 cccgaagacg gcaagatttg ccgtttggac gacgacaccg ttatcagcaa aaactccctg
    10861 tccgccgccc gtttttccgc cggttcggca gttcaggcag tcgatatggt catgaaacgc
    10921 aaagcgtggc acgccttttg cgccgcccgc ccgccggagc atcacgcgaa aagcggcaaa
    10981 gccggcggat tctgcctgct gaacaacgtt gccgccggcg tcatgcatgc cattgccgaa
    11041 taccgcctga aacgcattgc cgtcatcgat ttcgatgtcc actacggcga cggtacggca
    11101 gaaatattca aagacgatcc gcgcatcctg tttttcaacc tgtttgaaac cgaccttttc
    11161 cccttccccg aaaacaacgg tatgcccgac ggcggcaata tggtgcacct gcccttgccg
    11221 tcgggaacgg gcagccgcac attccgcgaa gccgtccgca gacagtggct gccccggctt
    11281 gccgcattca aacccgaact ggtgctgctg tcggcaggat tcgacgcaca ccgtctggac
    11341 gaatcgggca ggctcaacct gcacgaggcg gattttgcct ggctgacaca caaaatcatt
    11401 cagacggcat ccagctgccc cggcaaaatc gtatccgtac tcgaaggcgg ctacaccctt
    11461 gaacctctgg cacaatctgc tgccgagcat atccgcgtac tggcggggct gggcaaatcc
    11521 gatgccgcaa ccgcctatca gaaaacactg aacccaacaa aaaaacggtt tgccaaaccg
    11581 aaaaccggac aggtgcgcca acctacccaa tccgaccgtt acgatataaa tgccgtctga
    11641 agccgattcg gttttcagac ggcatcatta tttgacctga cgcaaacaga aagccttccg
    11701 cgtgcttaaa cacatctctt gagacctttg caaaaatccc caaaatcccc taaattccca
    11761 ccaaggcatt taggggattt cccatgaaca ccttcttccg gcaaaccgca caagccatga
    11821 ctgccaaaca catcggccgc ttcccgctat cggagttgga ccaggtgatt gattggcagc
    11881 cgatcgaaca atacctgaac cgtcaaaaaa cccgttacct ccgagaccgc cgcggccgtc
    11941 ccgcccatcc cctgtcgtcc atgttcaaag ccgtcctgcc cggacaatgg cacagcctct
    12001 ccgatcccga actcgaacac agcctcatca cccgcatcgg tttcaacctg ttttgccgtt
    12061 ttgacggacc gggcatcccc ggttgcagca ccttatgccg ctaccgtaaa ttccgctatg
    12121 cgcgggcagc ctatttcggg ctgctcaaag tgggtgcgca aagccacctg aaggcgatgt
    12181 gtttgaacct gttgaaagcc gccaacaggc taagtgcgcc cgctgccgcc taaaaggcgg
    12241 cccggatgcc tgattatcgg gtatccgggg gggattaagg ggatattcgg gtaaaattag
    12301 gaggtatttg gggcgaaaac agccgaaaac ctgtgtttgg gtttcggatg ccgaaggaag
//
LOCUS       DAXFZR010000065        12059 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000065 DAXFZR010000000
VERSION     DAXFZR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12059
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.065"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            187..690
                     /gene="def"
                     /locus_tag="ACFJAS_001613"
     CDS             187..690
                     /gene="def"
                     /locus_tag="ACFJAS_001613"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC3982813.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            778..1704
                     /gene="fmt"
                     /locus_tag="ACFJAS_001614"
     CDS             778..1704
                     /gene="fmt"
                     /locus_tag="ACFJAS_001614"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC3982814.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            1783..3042
                     /gene="rsmB"
                     /locus_tag="ACFJAS_001615"
     CDS             1783..3042
                     /gene="rsmB"
                     /locus_tag="ACFJAS_001615"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC3982815.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARRQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            3008..3607
                     /locus_tag="ACFJAS_001616"
     CDS             3008..3607
                     /locus_tag="ACFJAS_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC3982816.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            3607..5727
                     /locus_tag="ACFJAS_001617"
     CDS             3607..5727
                     /locus_tag="ACFJAS_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFC3982817.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            5720..6997
                     /locus_tag="ACFJAS_001618"
     CDS             5720..6997
                     /locus_tag="ACFJAS_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC3982818.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            7088..8275
                     /gene="dprA"
                     /locus_tag="ACFJAS_001619"
     CDS             7088..8275
                     /gene="dprA"
                     /locus_tag="ACFJAS_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC3982819.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            8301..8762
                     /locus_tag="ACFJAS_001620"
     CDS             8301..8762
                     /locus_tag="ACFJAS_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC3982820.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            8840..11146
                     /gene="topA"
                     /locus_tag="ACFJAS_001621"
     CDS             8840..11146
                     /gene="topA"
                     /locus_tag="ACFJAS_001621"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC3982821.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLAYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIEPPAPQE"
     gene            11367..11495
                     /locus_tag="ACFJAS_001622"
                     /pseudo
     CDS             11367..11495
                     /locus_tag="ACFJAS_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            11485..12029
                     /locus_tag="ACFJAS_001623"
                     /pseudo
     CDS             11485..12029
                     /locus_tag="ACFJAS_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2966 a         3330 c         3427 g         2336 t
ORIGIN      
        1 cataaccgtt ccccttatct gataaatttc ggtttgtctt gcttgattgg gttggaaaaa
       61 gcggcggcag cccctcggga tgtgccgcgt gataacaaat gttccgcatt ttaacatcga
      121 attatccgta ccatcacggt aattatgaaa aacaggcggc gtatccgccg aaggaaagag
      181 aaaattatgg ctttactgaa tatcttgcaa tatcccgacg agcgtctgca cacggtggca
      241 aagcctgtcg aacaagttga cgagcgcatc cggaagctgg ttgccgatat gtttgaaacg
      301 atgtacgaat cgcgcggcat cgggctggcg gcgacgcagg tcgatgtgca cgaacgcgtg
      361 gtcgtgatgg atttgaccga agaccgcagc gaaccgcgcg tgttcatcaa ccccgtcatc
      421 gttgaaaaag acggcgaaac cacttacgaa gagggctgcc tgtccgtacc gggcatttac
      481 gacgccgtta cccgcgccga acgcgtcaag gtcgaggctt tgaacgaaaa aggcgaaaaa
      541 ttcacgctgg aggcggacgg gctgctggcg atttgcgtgc agcacgagtt agatcacctg
      601 atgggcatcg tgtttgtcga acgcctttcc caactcaagc aggggcggat taagaccaaa
      661 ctgaaaaaac gtcagaaaca tacgatttga ttcgcttcgt catgccgtct gaacgctgca
      721 aggctttcag acggcacagc cttgtccgac aactttacgc acacgcagga acacgctatg
      781 aaagtcatct ttgccggcac gcccgatttt gccgccgccg ccttaaaagc cgttgccgcc
      841 gccggttttg aaatcccgct ggtgctgacc cagcccgacc gtccgaaagg gcgcggtatg
      901 caactgaccg ccccgcctgt caaacaagcc gcgctggaac tcggtttgcg cgtggcacag
      961 cccgaaaaac tgcgcaacaa cgccgaagcc ctgcaaatgc tcaaagaggt cgaggcggac
     1021 gtaatggtgg ttgccgcgta cggcttgatt ctgccgcagg aagtgttgga tacaccgaaa
     1081 cacggctgcc tcaatatcca cgcttcgctg ctgccccgtt ggcgcggcgc ggcgccgatt
     1141 cagcgcgcga ttgaggccgg cgatgccgaa accggcgtgt gcattatgca gatggacatc
     1201 ggtttggaca ccggcgatgt ggtcagcgaa caccgctacg ccatccaacc taccgatacc
     1261 gccaacgaag tccacgacgc gctgatggaa atcggtgcgg cggcggttgt tgccgatttg
     1321 caacagcttc aaagcaaagg ccgtctgaac gcggtcaaac agcccgaaga aggcgtaact
     1381 tacgcccaaa aactaagcaa agaagaagcg cgtatcgatt ggagcgaaag cgcggacatt
     1441 atcgaacgca aaatccgcgc cttcaacccc gtgcctgccg cgtgggtcga atatcagggc
     1501 aagccgatga aaatccggcg tgccgaagtg gtggcgcaac aaggtacggc aggcgaagtg
     1561 ttgtcctgtt cggcggacgg tttggtcgtt gcctgcggcg aaagcgcgct gaagattacc
     1621 gaattgcagc ctgccggcgg caggcgtatg aatattgcgg cgtttgcggc gggacggtct
     1681 attgaagcag gggcgaagct gtaaatccct tcagacggca ttccgatccg caaacgggaa
     1741 tgccgtctga aaccatcagt tgaagaaagc gaatcacata atatgagtat ggcacttgcc
     1801 caaaaacttg ccgccgacag cattgcggcg gttgccgaag ggcgcaacct tcaggacgtg
     1861 ttggcgcaca tccgcgcggc gcatccgcag ctgacggcgc aggaaaacgg cgcgttgcag
     1921 gatatcgcct acggctgcca gcgttatttg ggcagtttga aacatatgct cgcgcagatg
     1981 ctgaaaaagc ccatcgacaa tccgcagctc gaaagcctgc ttttggcggc gttgtaccaa
     2041 ctgcattaca cgcgcaacgc gccccacgcc gtggtcaacg aagctgttga aagcatagcc
     2101 aaaatcgggc gcgggcagta ccgttcgttt gccaacgcgg ttttgcgccg ctttttgcgc
     2161 gaacgtgaaa aattggcggc ttcctgcaaa aaagacgatg tggcgaaata caacctgccg
     2221 ctgtggtggg ttgcctacct gaaaaaccat tatccgaaac actggcacaa catcgccgcc
     2281 gcgctgcaat cccatccgcc gatgacgttg cgcgtcaacc gtcggcacgg caacgccgaa
     2341 agctatttgg aaaaactggc tgtggaaggt atcgcggcta aggcgttgga cgaatatgcg
     2401 gttacgttgg aagaagccgt gccggtaaac cgtctgcccg gttttgccga aggactggtg
     2461 tcggtacagg acttcggcgc gcagcaggcg gcgtatctcc ttaacccgaa agacggcgaa
     2521 cggattttgg acgcgtgcgc cgcgccgggc ggcaagacgg ggcatatttt ggaactggcg
     2581 gactgccgcg ttaccgcctt ggacatcgat gcaggccgtc tgaagcgtgt ggaggacaat
     2641 atcgcccgtt tgggctttca gacggcatcg gcggcgtgtg ccgatgcgcg ggatttggcg
     2701 gcgtggtatg atggaaagcc tttcgacgca atccttgccg acgtgccgtg caccgcttcc
     2761 ggcgtggcgc ggcgcaatcc cgacgtgaaa tggctgcgcc gaccgaccga cgcgctcaaa
     2821 accgcccgcc ggcaggaagc cctgctggac gcactttggc aggcgttgaa acagggcggc
     2881 agaatgctgc ttgccacctg ttccgtgttc gtcgaagaaa acgacgggca actgcaaaaa
     2941 ttcctcaacc gccatgccga tgccgaaccg atcgaatcgc gggtgctttt accgaacaaa
     3001 caccaagatg gcttttatta cgcgcttatt caaaagcatt aaacaatggc ttgtgctgtt
     3061 gccgatgctc tccgttttgc cggacgcggc ggcggagggc attgccgcga cccgcgccga
     3121 agcgaggata accgacggcg ggcggctttc catcagcagc cgcttccaaa ccgagctgcc
     3181 cgaccagctc caacaggcgt tgcgccgggg cgtaccgctc aactttacct taagctggca
     3241 gctttccgcc ccgacaatcg cttcttatcg gtttaaattg gggcaactga ttggcgatga
     3301 cgacaatatt gactacaaac tgagtttcca tccgctgacc aaccgctacc gcgttaccgt
     3361 cggcgcattt tccaccgatt acgacacttt ggatgcggca ttgcgcgcga ccggcgcggt
     3421 tgccaactgg aaagtcctga acaaaggcgc gttgtccggt gcggaagcag gggaaaccaa
     3481 ggcggaaatc cgcctgacgc tgtccacttc aaaactgccc aagcctttcc aaatcaacgc
     3541 attgacttct caaaactggc atttggattc gggttggaaa cctctaaaca tcatcgggaa
     3601 caaataatgc gccgcttcct accgatcgca gccatatgcg ccgtcgtcct gctgtacgga
     3661 ttgacggcgg caaccggcag caccagttcg ctggcggatt atttctggtg gatagtctcg
     3721 ttcagcgcaa tgctgctgct ggtgttgtcc gccgttttgg cacgttatgt catattgctg
     3781 ttgaaagaca ggcgcaacgg cgtgttcggt tcgcagattg ccaaacgcct ttccgggatg
     3841 ttcacgctgg tcgccgtact gcccggcttg ttcctgttcg gcatttccgc gcagtttatc
     3901 aacggcacga ttaattcgtg gttcggcaac gacacccacg aagccctcga acgcagcctt
     3961 aatttgagca agtccgcact ggatttggcg gcagacaatg ccgtcagcaa cgccgttccc
     4021 gtacagatag acctcatcgg caccgcctcc ctgtcgggca atatgggcag tgtgctggaa
     4081 cactacgccg gcagcggttt tgcccagctt gccctgtaca atgccgcaag cgggaaaatc
     4141 gaaaaaagca tcaatccgca ccaattcgac cagccgcttc ccgacaaaga acattgggaa
     4201 cagattcagc agaccggttc ggttcggagt ttggaaagca taggcggcgt attgtacgcg
     4261 cagggatggt tgtcggcagg tacgcacaac gggcgcgatt acgcgctgtt cttccgccag
     4321 ccgattcccg aaaatgtggc acaggatgcc gttctgattg aaaaggcgcg ggcgaaatat
     4381 gccgaattga gttacagcaa aaaaggtttg cagacctttt ttctggtaac cctgctgatt
     4441 gcctcgctgc tgtcgatttt tcttgcgctg gtaatggcac tgtattttgc ccgccgtttc
     4501 gtcgaaccca ttctgtcgct tgccgagggc gcaaaggcgg tggcgcaggg tgatttcagc
     4561 cagacgcgcc ccgtattgcg caacgacgag ttcggacgtt tgaccaagct gttcaaccat
     4621 atgaccgagc agctttccat cgccaaagaa gcagacgaac gcaaccgccg gcgcgaggaa
     4681 gccgcccgcc actacctcga gtgcgtgttg gatgggttga ctaccggtgt ggtggtgttt
     4741 gacgaaaaag gccgtttgaa aaccttcaac aaggcggcgg aacagatttt ggggatgccg
     4801 ctcgcccccc tgtggggcag cagccggcac ggttggcacg gcgtttcggc acagcagtcc
     4861 ctgcttgccg aagtgtttgc cgccatcggt gcggcggcag gtacggacaa accggtccag
     4921 gtggaatatg ccgcgccgga cgatgccaaa atcctgctgg gcaaggcgac ggtattgccc
     4981 gaagacaacg gcaacggcgt ggtgatggtg attgacgaca tcaccgtgct gatacgcgcg
     5041 caaaaagaag ccgcgtgggg tgaagtggcg aagcggctgg cacacgaaat ccgcaatccg
     5101 ctcacgccca tccagctttc cgccgaacgg ctggcgtgga aattgggcgg gaagctggac
     5161 gatcaggacg cgcaaatcct gacgcgttcg accgacacca tcatcaaaca ggtggcggcg
     5221 ttaaaagaaa tggtcgaggc attccgcaat tacgcgcgcg ccccttcgct caaactggaa
     5281 aatcaggatt tgaacgcctt aatcggcgat gttttggccc tgtacgaagc cggcccgtgc
     5341 cggtttgagg cggaacttgc cggcgaaccg ctgatgatgg cggcggatac gaccgccatg
     5401 cggcaggtgc tgcacaatat tttcaaaaat gccgccgaag cggcggaaga agccgatatg
     5461 cccgaagtca gggtaaaatc ggaaacgggg caggacggac ggattgtcct gacggtttgc
     5521 gacaacggca agggattcgg caaggaaatg ctgcacaatg ctttcgagcc gtatgtgacg
     5581 gataagccgg cgggaacggg actgggtctg cctgtagtga aaaaaatcat tggagaacac
     5641 ggcggccgca tcagcctgag caatcaggat gcgggtgggg cgtgtgtcag aatcatcttg
     5701 ccaaaaacgg tagaaactta tgcgtagcag cgatatttta attgtagacg acgaagtcgg
     5761 catccgcgac ctgctgtcgg aaatcctgca ggacgaaggc tattcggtcg cgttggcgga
     5821 aaatgccgaa gaggcgcgca agctgcgcca tcaggcgcgc ccggcgatgg tgctgctgga
     5881 tatttggatg cccgattgcg acggtatcac ccttttgaag gagtgggcga aaaacgggca
     5941 gctcaatatg ccggtggtgg tgatgagcgg gcatgccagc atcgataccg ccgtagaagc
     6001 gaccaaaatc ggcgcgatcg attttttgga aaaaccgatt tccctgcaaa agctgctgtc
     6061 tgcggtcgaa aacgcgttga aacacggtgc ggcgcaaatc gaaacgggac cggtgttcga
     6121 caaactgggc aacagtgccg cgattcaaga gatgaaccgc gaagtcgaag cggcggcaaa
     6181 gcgcacgtcc cctgtatttt tgacgggcga ggcgggttcg ccgtttgaaa ccgtggcgcg
     6241 ctatttccat aaaaacggta cgccgtgggt cagtccggca agggtcgaat atctgatcga
     6301 tatgccgatg gaactgttgc agaaggcgga gggcggcgtt ttgtatgtcg gcgacatcgc
     6361 ccaatacagc cgcaatatcc aaaccggcat tacctttatc atcggcaagg cagaacgctg
     6421 ccgtgtcagg gtgattgctt cgtgcagcta cgcggcaggt tcagacggca tttcatgtga
     6481 agaaaaattg gccgggcttt tttcggaatc ggtcgtccgt attccgcccc tgagtatgca
     6541 gcatgaagac attcccttcc tgatacaggg gattgcctgc aatgtggcgg aaagccaaaa
     6601 aatcacgcct accgcattca gtgacgatgc gcttgccgta ttgtcccgtt acgactggcc
     6661 ggggaatttc gagcagttgc aaaacgtcgt gacaacgctg ctgctggagg cggacgggca
     6721 ggaaatcggt atcggcgcgg cttcttcggc actggggcgc ggcgtgcctg ccgacggaac
     6781 ggggcatatg gcgtgcgggt tcgactttaa cctgccgctg cgcgaattaa gggaggaggt
     6841 ggagcggcgt tatttcgagt accacatcgc ccaagaaggt cagaatatga gcaaagtggc
     6901 gcagaaggtc ggtttggaac gcacgcacct ttaccgcaaa ctcaaacagc tcggcatcgg
     6961 cgtttcgcgc cgggcggggg aaaaaaccga agaataggcc cggacggccg gtttcccggc
     7021 tgcgggcttt tgttttcaga cggcatttgg agaaaatgcc gtctgaaatc gtaaggggat
     7081 ggattttatg acagaggacg aacgtttcgc gtggctgcaa ttggcgttta cgccctatat
     7141 cggcgcggaa agtttcctgc tgctgatgcg cagtttcggc agcgcgcaaa atgccttgtc
     7201 cgcaccggcg gaacaggttg ccccggcggt gcggcataag cacgcgcttg aggcttggcg
     7261 caatgcagaa aaacgcgctt tggcgcggca ggcggcagaa gcggcgttgg aatgggaaat
     7321 gcgggacgga tgccgcctga tgctgcttca ggatgaagat ttccccgaaa tgctgacgca
     7381 ggggctgacc gcgccgccgg ttttgttttt gcgcggcaac gtgcggctgc tgcacaagcc
     7441 ttccgccgcc atcgtcggca gccgccatgc cacgccgcag gcgatgcgga ttgccaaaga
     7501 tttcggcagg gcgttgggtg ggaaaggcat tcctaccgtg tcgggtatgg cttcgggcat
     7561 cgacaccgcc gcccatcagg gcgcactgga ggcagaaggc ggcaccatcg ccgtgtgggg
     7621 gacgggcata gaccgcattt atccgccggc caacaaaaac cttgcttatg aaatcgccga
     7681 aaaagggctg attgtcagcg agttccccat cggcacgcgg ccgtatgccg gcaactttcc
     7741 gcgccgcaac cgcctgattg ccgcgctgtc gcaggttacg ctggtggtcg aggccgcctt
     7801 ggaatccggt tcgctgatta ccgccggcct ggcggcagaa atggggcgcg aagtcatggc
     7861 ggtgcccggt tcgatagaca acccccacag caaaggctgc cacaaactga ttaaagacgg
     7921 agcgaaactg acggaatgtt tggacgacat cctgaacgaa tgcccggggc tattgcaaaa
     7981 tacgggtgct tcgtcatatt ctataaataa ggatacgccc gacacgggcc gccgcgccgt
     8041 tcagacggca tacgccccgc cgcctgccgc aaaaatgccg tctgaagggg cggcaggcgg
     8101 cacagccgtc ggcggcatac tggataaaat gggtttcgac cccatccatc ccgacgtgct
     8161 tgccggacag ttggctatgc ctgccgcaga tttgtatgcc gcactgttgg aattggaatt
     8221 ggacggcagc gttgccgcaa tgcccggcgg cagataccag cgtatccgaa cttgaacgca
     8281 ctttatatta aggaacacga atgaccgaag tcatcgccta cctcatcgaa catttccaag
     8341 atttcgatac ctgcccgccg cccgaagact tgggtatgct gcttgaagaa gcgggtttcg
     8401 atacgatgga aatcggcaac accctgatga tgatggaagt attgctcaac agctccgaat
     8461 tttccgccga acccgccggc agcggcgcat tgcgcgtgta cagcaaagaa gaaaccgaca
     8521 acctgccgca ggaagtgatg gggctgatgc agtatctgat tgaagaaaaa gccgtcagct
     8581 gcgaacagcg ggaaatcatc atccacgcgc tcatgcacat ccccggcgac gaaattaccg
     8641 tagataccgc caaagtgctg accctgctgc ttttatgggc aaacaagagc gagctgcccg
     8701 tgttggtcgg cgacgagctg atgagcgcgc ttttactcga caacaaaccc acgatgaact
     8761 gaagcggctt cagacggcgc cggcccgccc gatgccgtct gaaacaccgg catcaaaacc
     8821 accatccaga gaacgacaaa tggcgaaaaa cctattaatc gtcgaatccc cgtccaaagc
     8881 caaaaccctg aaaaaatatt tgggcggcga ttttgaaatc ctcgcgtcct acggacacgt
     8941 ccgcgacctc gtccccaaaa gcggcgcggt cgatcccgac aacggctttg cgatgaaata
     9001 ccaactcatc agccgcaacg gcaaacacgt cgatgccatc gtcgccggag ccaaagaagc
     9061 cgaaaacatc tacctcgcaa ccgacccgga tagggaaggc gaagcgattt cttggcatct
     9121 tttggaaatc ctcaaatcca aacgcggcct gaaaaacatc aagccgcagc gtgtcgtgtt
     9181 tcacgaaatc accaaaaacg ccgtgctcga tgccgtcgcc catcctcgcg aaatcgaaat
     9241 ggatttggtc gatgcgcaac aagcccgtcg cgctctggac tatctggtcg gtttcaacct
     9301 ctcgccgttg ctgtggaaaa aaatccgacg cggtttgagc gcgggccgcg tgcaaagccc
     9361 tgctttgcgc ctgatttgcg aacgcgaaaa cgaaatccgc gcgtttgaag cgcaggaata
     9421 ttggacggtg catctcgaca gccataaagg ccgcagcaag ttcaccgcca aactcgccca
     9481 atacaacggc gcgaaactcg aacaattcga cctgccgaac gaagccgctc aagccgatgt
     9541 gttgaaagaa ctcgaaggca aagaggctgt cgttactgcc attgaaaaga aaaagcgcag
     9601 ccgcaacccc gccgcgccgt ttaccacatc caccatgcag caggatgccg tgcgcaaact
     9661 cggtttcacc accgaccgca ccatgcgtac cgcccagcag ctttacgaag gtatagacgt
     9721 agggcagggc gccatcggtc tgattaccta tatgcgtacc gacagcgtga atttggctga
     9781 cgaagcgtta accgaaatcc gccattacat tgaaaacaaa atcggcaaag aatatctgcc
     9841 gagtgccgcc aaacaataca aaaccaaatc caaaaacgcc caagaagccc atgaagcgat
     9901 ccgcccgact tccgtgtacc gcacgcccga aagcgtcaaa cccttcctga gtgcagacca
     9961 gttcaaactc tatcaaatga tttggcagcg caccgtcgcc tgtcagatga cgcccgccaa
    10021 attcgaccaa accaccgtcg atattaccgt cggcaaaggc gtgttccgag taaccggaca
    10081 agtgcaaacc ttcgcaggct tcctcagcgt ttacgaagaa agcagcgacg atgaagagag
    10141 cgaagacggc aaaaaactgc ccgaaatgag cgaaggcgac aaattgccgg tcgacaaact
    10201 ctacggcgaa caacacttca ccactccgcc gccgcgctac aacgaagcca cactggttaa
    10261 agcccttgaa gaatacggca tcggccgccc ctcgacctac gccagcatca tctccacgct
    10321 caaagaccgc gaatacgtta cccttgagca aaaacgcttc atgcccaccg acacaggcga
    10381 catcgtcaat aaattcctga ccgaacactt cgcccaatac gtcgattacc acttcaccgc
    10441 caaactcgaa gaccagcttg acgaaatcgc cgacggcaaa cgccgctgga ttcccgtgat
    10501 ggacaaattc tggaaaccgt tcatcaaaca agtggaagaa aaagagggca tcgaacgcgc
    10561 caaatttacc acgcaggaac ttaacgaaac ctgcccgaaa tgcggcgaac acaaactgca
    10621 aatcaagttc ggcaaaatgg gccgcttcgt cgcctgtgcc ggctatcccg aatgcagcta
    10681 cacgcgcaac gtcaacgaaa ccgccgaaga agccgccgag cgcatcgcca aagccgaggc
    10741 tgaacaagcc gaactcgacg gacgcgaatg ccccaaatgc ggcggtcggt tagcgtataa
    10801 atacagccgt accggcagca aattcatcgg ctgcgccaac taccccaaat gcaaacacgt
    10861 cgagccgctg gaaaaaccga aagacaccgg cgtccaatgc ccgcaatgca aaaaaggtaa
    10921 cctcgtcgag cgcaaatccc gctacggcaa actgttttac agttgcagca cctatcccga
    10981 ctgcaactac gccacttgga acccgcccgt tgccgaagaa tgcccgaact gccattggcc
    11041 ggtcttgacc atcaaaacca ctaaacgccg gggtgtggaa aaagtctgcc cgcaaaaaga
    11101 atgcggctgg aaagaacaaa tcgaaccgcc tgcaccgcaa gagtgaagcg gttgggatta
    11161 agatgaaaga aaatgccgtc tgaaaggggt ttcagacggc atttgtaaat tagaaggggc
    11221 tgtcccagat ggctggggaa atttaaatta agtcagaatt atccctatga gaaaagccgt
    11281 ccaagccggt acaaacaaaa gaaacccatc ggactgtttg ccgcaggggt aactgcaaga
    11341 acggcagcag ggttggcagc agttaatcaa aacacggcag cctgttattt tctatggcga
    11401 aatagtgttt ggcacatcat cgggaaaact tcctttacac gcatttggac gaaatctgcg
    11461 aaattatgga ggcatacgac atatcgttta cgattactta tccgtcaaaa cagtccgcat
    11521 ttggaaatgt atgacggcga agcaggggcg ggtgaaagtt atttccgtaa aggcagacgc
    11581 ggccgcagtg ccgccggcaa agccgccgta ttcgggcttt tgaagcgcaa cggcaaggtt
    11641 tgtacggtta ctgtcggaca ctcgaaccgc tattttattg cctattatcc gcggacgggt
    11701 gaaacccggc agcattgttt atacggattg ttcgtagtta cgatgtatca gattgtaagc
    11761 ggatttggcc gtttccgtat caatcgcggc acacattttg ccgaacggca aaaccatatc
    11821 aacgcaattg ggaacttttg gaatcgggca aacgtcattt gcgcaagttt gacggcattc
    11881 ccaaagagca tttcgggctg tatttgaagg agtgcggacg gcgttttgac aacagtgggg
    11941 taaaagttcg aatttccatt ttaaaacaat tggtaaagca ggatttatcc cggttggccg
    12001 ggattttaaa aaataaatac attctttaac aaaacaaatc atatcccggc aaaacaaat
//
LOCUS       DAXFZR010000066        11668 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000066 DAXFZR010000000
VERSION     DAXFZR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.066"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..996)
                     /locus_tag="ACFJAS_001624"
     CDS             complement(<1..996)
                     /locus_tag="ACFJAS_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC3982822.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLV"
     gene            complement(989..1990)
                     /locus_tag="ACFJAS_001625"
     CDS             complement(989..1990)
                     /locus_tag="ACFJAS_001625"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3982823.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(2021..4292)
                     /locus_tag="ACFJAS_001626"
                     /pseudo
     CDS             complement(2021..4292)
                     /locus_tag="ACFJAS_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
     gene            4407..5423
                     /gene="galE"
                     /locus_tag="ACFJAS_001627"
     CDS             4407..5423
                     /gene="galE"
                     /locus_tag="ACFJAS_001627"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC3982824.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITCRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            5424..6464
                     /gene="rfbB"
                     /locus_tag="ACFJAS_001628"
     CDS             5424..6464
                     /gene="rfbB"
                     /locus_tag="ACFJAS_001628"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC3982825.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            6569..7435
                     /gene="rfbA"
                     /locus_tag="ACFJAS_001629"
     CDS             6569..7435
                     /gene="rfbA"
                     /locus_tag="ACFJAS_001629"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC3982826.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYQVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFYDNRTVEFAKRLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQDLHIACLEEIAWRNGWLTKKDVETRAKHLEKT
                     AYGQYLLHLIGK"
     gene            7574..8131
                     /gene="rfbC"
                     /locus_tag="ACFJAS_001630"
     CDS             7574..8131
                     /gene="rfbC"
                     /locus_tag="ACFJAS_001630"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC3982827.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(8175..10193)
                     /locus_tag="ACFJAS_001631"
     CDS             complement(8175..10193)
                     /locus_tag="ACFJAS_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC3982828.1"
                     /translation="MNKSLSGSVEKYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLVLLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(10388..11509)
                     /gene="dnaJ"
                     /locus_tag="ACFJAS_001632"
     CDS             complement(10388..11509)
                     /gene="dnaJ"
                     /locus_tag="ACFJAS_001632"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC3982829.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         2868 a         3212 c         2849 g         2739 t
ORIGIN      
        1 tactaaaaac agaatttcct tatggctttg cttcttttct tttcctgtaa ccttataaac
       61 gtgaggcgtt tccaagattt gtacatttcg cccattttta cctaacattt cttctaattc
      121 atctcggttt ggatatgaag cattgttgta gcttaacagc caatatttaa atggtttcaa
      181 atgattgatt acccttttaa ataattcgac agcctgcttt ttatccatga aatgattggc
      241 aaatggtttg ggtatggatg atgtaatgta actgtccaat aatccgtaaa agccaaaata
      301 attgttcatc gtgcctgtgt aaggtgggtc taaatagacg gcatcagctt gtatattagg
      361 taataaatca aatatatctt gattaaaggc gtgatgccgc cagccattat tgaaaacagc
      421 ttggttatag tcatccaaat tttgcagaaa atgactttga aaactttcat tgtggtaggc
      481 gcgtcgacgg ccatatttag cataactgta ttcttcatca cgcagttgtt taactttttc
      541 ccaaggaatt gtgaatcgtg aatagggcat tttacgtatc atggcgcgac gcattaaagt
      601 aaacgccaaa gcacgtttat actggttatt cagcttccct atatttttac ggtacaaatc
      661 aagttgttgg cattcatcgt gaaaatagaa tttttcggaa taacgctgac tcataaaacc
      721 ggcatgcggg ctgcctgaaa aaatcattgc gacatcatcg tcatttaatg tttcatggtt
      781 gttttctatt aatgctaaag caatttggta attaattgcc aaaatatcgt tagtaatcac
      841 acgatagccg cgttttttgg cttcgtaggc aaaggaacag cctccactaa atacatctgc
      901 aactgtatct acatcagacg gaagctggtc acaaatccag gaagctattt tctctttatt
      961 accgatatag ttaattttcg gatatttctt attcattttc actcattttt aaaatacttt
     1021 cagcaatagc ttttgccaat aaaggcggca cggcgttacc aacctgctgc tgctgggcaa
     1081 ttttgctgcc gcaaaaaata aaattatcag ggaaagattg taaggcagct aattcacgaa
     1141 cggttaaagc ccgattctgt tcatagtgaa aaactttgcg catatctcct gtaatacaaa
     1201 cggctggttt gttgctgttg taacggatgt atttacggat atcacctgtt ttcggacgta
     1261 atggttcagg aatatcgtta cggttacctc catttttaac aaatgccatt ttttctaaca
     1321 tttgtgccga atgattcata gcttcatgat ttgcaacgtg tggattgctt tcgccagcag
     1381 ccagttttgg aaaatgtcct attgctgatc caacagtctg atgggaaatc tgcaaaggtt
     1441 cgggaaagga aattttgcct ttatccctcc tcccgataaa tatcactcgg ctacgtatct
     1501 gagggacacc gaaatcggct gcactcagta tcttacattc caccgaataa ccgatattct
     1561 gaaatgcttg aataatctca atacgtgttt tacctgaatt gtgtgtatag agtcgcgcta
     1621 cattttccat aacaaaaaaa tatggttgga caattttaac tattcggaca aactctttaa
     1681 ataaatggtt gcgtgggtca tctgtaaatg tccgtccaat ctttcctgcc atactaaaac
     1741 cttgacaagg tggtcctcca ataaccaaat caactgattg tccgttaaga caattggtta
     1801 aatcttgttc ggttagtgtg gttaaatctt tttgcagtaa ttgatgacgg gggaagttgg
     1861 tacggtaagt ctgacaataa tcagactcca tttcaacaga aagcaattgt tggaatccgg
     1921 cttgttcaaa acccaaggat aggcctcctg ctcctgagaa aaggtcaata taggtaagtg
     1981 gtgtgtgcat aagtcaaaat ccataaactc ttcgattatt ttaccgcttc agcttcgcaa
     2041 acgcatccgc catcgccgaa ttggtcgggg cgcggtcgtt gcgttggggt ttgcggccgg
     2101 ctgtccgttc gcggctgcgg ttttcagacg gcattttatg ttttgcgccg cccggttcgt
     2161 catccaagcg catggtcagc gcgatgcgtt tgcgtgcggc atcgacttcc agcactttca
     2221 ctttcaccac gtcgccggct ttcaccactt cgcgcgggtc ttggacgaac ttattagaca
     2281 gggcggagat gtgcaccaag ccgtcctgat ggacgccgat gtccacgaac gcgccgaagt
     2341 tggcgacgtt ggagaccacg ccctcgagta tcatgccgac ttgcaagtcg ctgatttcgt
     2401 ggatgccttc ggcaaacgat gccgtctgaa acgcaccgcg cgggtcgcgg ccgggttttt
     2461 ccagctcgga caggatgtcc aaaatagtcg gcaggccgaa gcgttcgtcg gtgaagtcgg
     2521 atgctttgat ttgcttcaca cgctcgcggt tgccgatgag ttcggcggcg gtaatgcctt
     2581 gttgcgccag cattttggcg acgacgggat aggcttcggg gtggacggcg ctcgcgtcca
     2641 gcggctcttt gccgccgtta atccgcaaaa agcctgctgc ttgctcgaag gttttttcgc
     2701 ccaagcgcgg tacttttagc aatttttttc ggctgtcgaa cgcgccgttt tcatcacggt
     2761 aggcgacgat gttttgggca agggtttgat ttaaaccgga aatccgcgcc aagagcgggg
     2821 cggaggcggt gttcgcgtcc acgccgacgg cgttcacgca gtcttcgacc accgcgtcca
     2881 gcgatttggc gagccggctt tggttcacgt cgtgctgata ctgccccacg ccgatggatt
     2941 tggggtcgat tttgaccaac tcggcaagcg ggtcttgcag cctgcgggcg atggacactg
     3001 cgccgcgcag ggaaacatct aagtcgggaa actcgcgcgc cgccagttcg gacgcagaat
     3061 aaatcgacgc gccggcttcg gaaacgacga ttttgtgcag gctgctttcg ggcattccgc
     3121 gcaccagttc gcccgcgatt ttgtcggttt cgcggctggc ggtgccgttg ccgatggcga
     3181 tgagcttcac accgtgttgt ttaatcaggc gcgacaacgt tgccaacata ttgttttctt
     3241 gatgcaaata gacaatgacc gtatccagca gtttgcccgt gtcgtccacc acggcgcatt
     3301 tcacgccgtt gcggtagccg gggtcgagtc ccaaagtggt cagccgcccg gcgggcgcgg
     3361 cgagcagcaa gtctttgaga ttgcgggcga acacggtaat cgcgtcagtg tcggcagctt
     3421 ctttcaaacg attgagagct tcgagttcca gcgacaaaaa gattttcgcg cgccaagtca
     3481 ggcgcacggt gtcgcgcagc catttgtggc cgtctgaaac cttgaagcgg cgggcgatga
     3541 tttgctcgta ttcgctttgc tgcgtaatcg gcgtgtcgtc gggctggtat ttgagcgcga
     3601 tgttcaacac gccttcgttg cgaccgcgca aaaccgccag cgcgcggtgg ctgggcatgg
     3661 cgcgcacggg ttcgcggcgg tcgaaataat cgctgaattt ttcgccttcg gtttctttgc
     3721 cttcaacgac ttgcgtgtgg atttcggctt cgttccacag cttgtcgcgc agcgtgccga
     3781 tgagttccgc gtcttcggca aactgctcca tcagaatcgc gcgcgccatc cagcgcggct
     3841 ttggcgtcgg ggatgttttc gttcaggtag ccttgcgcgg cggcttccac gtcctgcggc
     3901 tgttcggcaa gcaacacgtc cgccagcggc tgcaaaccgt gttcgcgagc gatttgcgct
     3961 ttggtgcggc gtttgggctt gtaaggcaga tacaggtctt ccagcgcggt tttgttgtcg
     4021 gcggcttcga tttgcgccct gaggtcgtct gaaagcttgc cttgctcttc aatgcttttt
     4081 aacacaacgg ccttgcgctc ttccaactcg cgcaggtatt gcagccgctc ggcaagctgg
     4141 cgcagctgcg tatcgtccag cccgcccgtg gcttctttac ggtaacgcgc gataaagggg
     4201 acggtcgcgc cgtcgtccaa aagctcgata gcggcattga tttgcacggt agtggcggag
     4261 agttcttggg agaggattcg ggtaatgttc ataaattgcc tccgtgatgc cgtctgaaca
     4321 gccgacggcg caatgtgggt aatcgtttgg aaaagatgaa gataatactg atatactttg
     4381 cccgatacaa tctgaaagga atgtttatga ccgtcctgat taccggcggc accggcttta
     4441 tcggttcgca caccgccgtc tcgctcgtcc aatccggtta cgatgccgtg attttggata
     4501 atctgtgcaa ctcgtctgcc gccgtcctcc cgcgccttcg gcaaattacc tgcagaaaca
     4561 taccgtttta tcagggcgac atccgcgact gtcagatttt gaggcagatt ttttcagaac
     4621 atgaaatcga atccgtcatc cattttgccg gtttgaaggc agtgggggaa agcgttgccg
     4681 agccgacaaa atattacggc aacaatgttt acggcagcct ggtgctggcg gaagaaatgg
     4741 cgcgcgcggg cgtgttgaaa atcgtattca gctcgtcggc aaccgtttac ggcgatgcgg
     4801 aaaaagtccc ctatacggaa gatatgcgcc cgggcgatac cgctaatcct tacggtgcgt
     4861 ccaaagcgat ggtggagcgg atgttaaccg acatccaaaa agccgatccg cgttggagcg
     4921 tgattttgtt gcgctatttc aacccgatcg gcgcgcacga aagcggactt atcggcgaac
     4981 agcccaacgg cgttcccaac aatcttttgc cctatatctg tcaagtggct tcgggcaggc
     5041 tgccgcaact gtcggtattc ggcggcgact atccgacccc cgacggtacg ggaatgcgcg
     5101 actacatcca tgtgatggat ttggcagaag ggcatatcgc ggcaatgaag gcgaaaggcg
     5161 gcgttgccgg cgtacatttg ttcaacttgg gttcgggacg cgcctattcc gttttggaaa
     5221 tcatccgcgc ctttgaggcc gcatccggtt tgcacattcc ttaccgaatc caaccccgcc
     5281 gcgccggcga cttggcgtgt tcctatgccg acccgtccca taccaaacaa caaaccggct
     5341 gggaaaccaa acgcggcttg cagcaaatga tggaagattc gtggcgttgg gtcagccgca
     5401 accccggcag atatggggat taaatgcaaa ccgcaggcaa aaaaaacatc ctcgttaccg
     5461 gcggcgcggg ttttatcggc tcggcagtcg tccgccatat tatccaaaac acccgagatt
     5521 ccgtcgtcaa cctcgacaag ttaacctacg cgggcaatct cgaatcgctg accgacattg
     5581 ccgataatcc ccgctacgct tttgagcaag tggatatttg cgaccgcgcc gaactcgacc
     5641 gcgtgttcgc gcaataccgg cccgatgccg tgatgcactt ggcggcggaa agccacgtcg
     5701 accgcgccat cggttcggca ggcgaattta tccgaaccaa tatcgtcggc acattcgacc
     5761 tgctggaagc tgcgcgcgcc tattggcagc aaatgccgtc tgaaaaacgc gaagccttcc
     5821 gtttccacca tatttctacc gatgaagtct atggcgattt gcacggcaca gacgatttgt
     5881 ttacagaaac cacgccatac gcgccgtcca gcccctactc cgcctccaaa gccgccgccg
     5941 accaccttgt ccgcgcgtgg cagcggactt acaggctgcc ctccattgtc agcaactgtt
     6001 ccaataatta cggcccccga caattccccg aaaaactcat tcctttgatg attctgaacg
     6061 cactttccgg caaaccactg cctgtgtacg gcgacggggc gcaaatccgc gactggctgt
     6121 ttgtcgaaga ccacgcgcgc gcgctgtatc aggtcgttac cgaaggtgtt gtcggcgaaa
     6181 cctacaatat cggcggacac aatgaaaaaa ccaatctcga agtgatcaaa accatctgcg
     6241 ccctcttgga agaactcgcc cccgaaaaac cggccggcgt ggcacggtat gaagatttaa
     6301 ttactttcgt acaagaccgc cccggccatg atgcccgcta cgccgtcgat gcggcgaaaa
     6361 tcaggcggga tttgggctgg ctgcctttgg aaacttttga atccggcctc cgcaaaaccg
     6421 tgcaatggta tttggacaac aaaacccggc ggcaaaacgc ataaaacggc aactgccgtt
     6481 cggaacgctt aggtacgggc aaatagtttt cagacggcat cccgacgcaa tgccgtctga
     6541 aaacccatcg caaaggaaga aagaaaagat gaaaggcatc atactggcag gcggcagcgg
     6601 tacgcgccta tatccgatta cgcgcggcgt atccaaacaa ctgctgcccg tgtacgacaa
     6661 accgatgatt tattacccct tgtcggtttt gatgctggcg ggaatccgcg atattttggt
     6721 aattaccgca cccgaagaca acgctgcctt ccaacggcta ctcggcgacg gctccgactt
     6781 cggcatccgc ctgcaatatg ccgttcaacc cagccccgac ggcttggcgc aggcatttat
     6841 catcggcgaa gaatttatcg gcaacggcaa tgtctgcctg attttgggcg acaatatttt
     6901 ctacggtcaa tcatttacgc aaacattgaa acaggccgcc gccaagaccc acggcgcaac
     6961 cgtattcggc tatcaagtca aagaccccga gcgtttcggc gtagtcgaat ttgacgaaaa
     7021 cttcaacgcc ctgtctatcg aagaaaaacc gcaacagccc aaatccgatt gggcggtaac
     7081 cggcttgtat ttctacgaca accgcaccgt cgagttcgcc aaacggctca aaccgtccgc
     7141 ccgaggcgaa ttggaaatct ccgacctcaa ccaaatgtat ttggaagacg gttcattgtc
     7201 cgtccaaatc ttagggcgcg gtttcgcatg gttggacacc ggcacccacg aaagcctgca
     7261 cgaagctgct tcattcgtcc aaaccgtgca aaatatccaa gacctgcaca tcgcctgcct
     7321 ggaagaaatc gcttggcgca acggttggct gacgaaaaaa gatgtagaaa cacgcgcaaa
     7381 acatttggaa aaaaccgcct acgggcaata cctgctgcac ctaatcggca aataaatacc
     7441 gtctgaacgc cccgacggct ttcagacggc atccaaaccg tgccgcgccg gcaccttaac
     7501 cgacctgccg cacggcgcgc ataaaaaatc aaaccgcaaa aaaacagcgg taacgatacc
     7561 gccaaaggac tttatggaca tcatcgacac cgccctcccc gacgtaaaac ttttaaaacc
     7621 gcaagtcttc acagacgggc gcggtttttt tatggaaaca ttccgcgacg gatggttcaa
     7681 agaaaatatt gccgatcgaa ctttcgtgca ggaaaaccac tccaactcca gcaaaggcgt
     7741 attgcgcggc ctgcactacc aaaccgaaaa cacccaaggc aaactcgtac gcgtagttgt
     7801 cggcgaagtg ttcgacgtgg ccgtcgatat gcgcgaaggt tcgccgactt tcggcaaatg
     7861 ggcgggcgca accttgtcgg cgcaaaaccg ataccagctt tggatacccg aaggcttcgc
     7921 acacggtttt tgcgttttgg gcgacgcggc ggaagtcgtg tacaaatgca cggactatta
     7981 caaccctgaa accgaacagg ttttaatatg gaacgacccg gcaatcggca taggctggcc
     8041 gcttcaaacc gcgccgctgc tttcgcccaa agaccttgcc ggcaaaacgt gggcacaagc
     8101 cgaaaagctc cgccttacgc tttcccgata aaaaatgccg tccgaacgtt tcagacggca
     8161 ttttctccga caggctacct acccgccttc agtacgcgct gtgcaaagaa aaacatcccg
     8221 gtaacgaaga acgccaagcc caaccaagaa gcggcggcat cccagttttg cagattcaac
     8281 gcgagcggcg catccgagcc gccgttggtc acagagaagg caataataaa cgccataatt
     8341 acaccaatca ggctttcacc gacaatcagg cctgccgcga aaagcgtacc gatacggtcg
     8401 gcgtttttca gacggccttc gcggttttcc gccttcttgc caatgatgtg tttcaacacc
     8461 gccgccaaca ccgcgcctgc cacgatgggc atattgacgg acggcggcag ataaatgccc
     8521 ataccgaccg caaggacggg cagggcaagt ttaccgcctg atgatttttt caacaccaaa
     8581 tcgacgacga ttaataccgc tccaatcgcg ataccggtaa agatgtacgc ccattcgagg
     8641 ttgtgggcga aaatgcccga cgcaatggtc gtcatcaaag tcgcttgagg ggctgccaaa
     8701 gcctgcgccg catccatgcc ttcgcgcggc attgcgccgg taaagccgta ggcttcgtaa
     8761 agcagttcca acacgggcga aataaccagc gcaccaacga tacagccgat aatcagggcg
     8821 acttgctgcc gccaaggcgt agctttgagc aggtagccgg ttttcaagtc ttgcaggttg
     8881 tcgttggaaa tcgaagccac gcagattact gccgatccgc aaaaaagcgt caacgccagt
     8941 aagaatttgc ggttggcctc atccgccaac aaacctccgg attcgcctac cagcagcaaa
     9001 accagtgaaa taacgacgat ggacacgatg cccacgccgg aaatcgggct ggaagacgag
     9061 ccgaccaaac ctgccatata accgcaggcg gcggcgacca aaaagccgat gacggaagcc
     9121 aaaagcgtgc aaaccgccac caaaagccac gccatgccgc ccgtaatgtg cgaatcgccg
     9181 ataaagtggt aaaacgacac gcctagaata aacatcatag acaacaccca gaaaatcatg
     9241 gctttaggcg acaaatcctg ttcggcgcgt tctgtagcgg gcgcaccgcc gccaaaactc
     9301 ttgaacgaca tcttcatgcc ttccaccatt ggcttgagca gcatcaaaag cgtccaaacc
     9361 gccgcgatac ctatggttcc cgcaccgata aaacgcactt tttccttcca cagcttcatc
     9421 gcaaacgccg ccatttccat atcggaaggt tgcggaatgt gtgaggagaa atacggcacg
     9481 gcaatacccc aagcaatcga aatgcccagc aggatggcga tgccgcccgt cagtccgacc
     9541 aaataacccg cgcccaacaa gaccagtgaa aagcccatcg gcagttggaa aattgccgta
     9601 ccgcttttaa accaataact cgcgctatcg gcaatcacac gcagacctcc ggcgcaaaag
     9661 ctcatcaatc ccgccaacgc gccgccggcc gccagctctt tgataccgct gccgccctga
     9721 cggtcatccc cttcttcatg accgcccact ttcaaaattt cagcagccgc cacaccttcc
     9781 ggataaggca aatcgctttt caccaccatc gcgtaacgca gcggaatggt gaaaatcacc
     9841 cccaaaatcc cgccggcaat acataaaagc gtcgtctgcc aaaacgggaa accgctccag
     9901 tagcccgcca tcagcaaacc gggcaggacg aagatgatgg ttgaaagcgt acccgcagcc
     9961 gaggcttggg tctgcaccat gttgttttcc aaaatattgc tgcctttgaa aaattttaaa
    10021 accgccatcg aaatcaccgc cgccggaatc gacgaggcaa aggtcagccc gactttcaaa
    10081 ccgaggtaaa cgttagacgc agtaaaaatt acagtgatca atgcgccgag tatcatgcct
    10141 cggagcgtca gttcgcggta tttttctacc gaaccggata aagatttatt cattattctt
    10201 cctttgacaa cagacgttca catattgttg gcatcacgcc atgatgtcaa gttttaaaaa
    10261 gaacagttaa aaacaattat cccaccctgc ccatacccaa ttgaaaataa aaactatttc
    10321 caaacgaaca aaaacagccg tatcagggag gttccccgat acggctgctt gtttccgaac
    10381 cttaaaatca atcaaacaaa tcgcgcagct tgtctaaaaa cgatttcttg cgcggcgttt
    10441 ggttttccaa gcccgtagaa atccgctcaa attcttccaa aagctctttt tgacggtcgg
    10501 tcaaattgac aggcgtttcg acaacaatat ggcagtacaa atcgccggtc gcgctgctgc
    10561 gtaaagattt gacaccctta cctttcacgc gcatcctcct gccggtttgg gtttctttgg
    10621 ggacggtgag cttgactttg ccgtccaagg tcggcacttc taactccccg cccaaagcag
    10681 ccgtggcaaa actaatcggc agttcgcaat gcaaatccaa accatcgcgt tggaaaatct
    10741 tatgcgcccg aatgcggacg gttacataca ggtcgccggc aggcgcgccg tgcatacccg
    10801 gcccgccttc accgctcaaa cggatacgct gcccgtcatc gataccggcg ggaatattga
    10861 cttccaccgt cttgaccgcc ttattccgcc ccacgccacg gcatttgacg caaggttctt
    10921 taatatgttt gcccgcaccg cggcaggtcg gacaagtctg ctgcatacgg aaaatcgcct
    10981 gctggatgtg caccgtaccc gaacctttgc aagtcgggca ggtttccggg gatgcccccg
    11041 gtttcgcgcc gctgccgttg cagacatcac acgcttcata agtcggaata ttgatgcgtt
    11101 tcttcacacc ttttgcggct tcttcaagcg tgatttcgat accgacctgc acgtcctcac
    11161 cctgataatc aggctgggcg cgccccgaac cacctccaaa catttggctg aaaatatccc
    11221 caaagtcaaa accctgcgca ccgccaaatc cgccaaaccc tccgaagccc ccctgtccgc
    11281 cgccttcaaa cgccgcatga ccatactggt cgtacatggc acgcttttcc ttgtcggaca
    11341 aagtttcata cgccttttgt acttctttaa acttctcttc cgcctcttta ttgtcaggat
    11401 tgcggtcggg atggtatttc atcgccaatt tacggtaggc ttttttaatc tcatcatcgg
    11461 tagctgctct tgccacaccc agcgtcgcat aaaaatcttg attactcatt ttttcatcta
    11521 aaaataaaat cacggcttaa aatgagggca attgcgcaaa acacaagaca aacagactga
    11581 gacctttgca aaattcccct aaaatcccct aaattcccac caaggcattt aggggatttc
    11641 ccatgagcac cttcttccgg caaaccgc
//
LOCUS       DAXFZR010000067        11664 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000067 DAXFZR010000000
VERSION     DAXFZR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11664
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.067"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            293..1147
                     /locus_tag="ACFJAS_001633"
     CDS             293..1147
                     /locus_tag="ACFJAS_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFC3982830.1"
                     /translation="MSNGARWTVTNDSMLKELDLSEDAQVEFSDNNKFVKVSVSKLKG
                     DGGVFKMYGDIVKGESDKLITRKGSEGTHIIEYMDDAKAKTTGREYLKLVENKGNQED
                     NKASNKASYKLNVRCTEQGGWCFALGESGASKKVNISTDGKRDFYLYPDTLTPGASSS
                     VLFGEALYQLNAVSDETLVQRMGEIHADGTPQEDNNVWIKRVGGKFSGSRSDYRVGGY
                     GNRYWGFAGGFNRTGFGDKWIHYKGLMLRHLQSSYASEDYVGSGKILRQGGRCLFRLA
                     QPGKQGLL"
     gene            1237..1695
                     /locus_tag="ACFJAS_001634"
     CDS             1237..1695
                     /locus_tag="ACFJAS_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3982831.1"
                     /translation="MPICFPPKPVGGWKNKTAAKTYRWQPEVQLSYWFTRGYGFPLSN
                     GLSAETDNFRSLMGRFGFRAGVDGLDGGRLNIYGKLMYKREFIGTIRHRFNGSAVEEF
                     KHRGGWLEYGLGVVRRNAGNGRQLYFEAQRSSMHTMRQNWQVNMGVRSMF"
     gene            complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="ACFJAS_001635"
     CDS             complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="ACFJAS_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC3982832.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3688..5211)
                     /locus_tag="ACFJAS_001636"
                     /pseudo
     CDS             complement(3688..5211)
                     /locus_tag="ACFJAS_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(5518..6324)
                     /locus_tag="ACFJAS_001637"
     CDS             complement(5518..6324)
                     /locus_tag="ACFJAS_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC3982833.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6376..6627)
                     /locus_tag="ACFJAS_001638"
     CDS             complement(6376..6627)
                     /locus_tag="ACFJAS_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982834.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6729..7499
                     /locus_tag="ACFJAS_001639"
     CDS             6729..7499
                     /locus_tag="ACFJAS_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982835.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7645..8814
                     /gene="metZ"
                     /locus_tag="ACFJAS_001640"
     CDS             7645..8814
                     /gene="metZ"
                     /locus_tag="ACFJAS_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC3982836.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLECVGDLIDDLKQALAR"
     gene            8909..9115
                     /locus_tag="ACFJAS_001641"
     CDS             8909..9115
                     /locus_tag="ACFJAS_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC3982837.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            9511..9735
                     /locus_tag="ACFJAS_001642"
     CDS             9511..9735
                     /locus_tag="ACFJAS_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982838.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            9764..11050
                     /locus_tag="ACFJAS_001643"
     CDS             9764..11050
                     /locus_tag="ACFJAS_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC3982839.1"
                     /translation="MADALADGGAAATAVGCPPRLIGGEQNKTPNPKGAEKTENQEFQ
                     FEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTKVTGKGLVSDTEFVV
                     RYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHYGGQCETMLVELNGT
                     GCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQAMLDHDNGHYDVHNM
                     RPKSECRGTAWRNEDGSGKTFYIGKRGNSKFTRVYEKGRQLGDVDSPWVRFEGDIEIP
                     LDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKLFHARNQVGKMVNFL
                     RDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHEEQKAINALNIETLF
                     DDLIEEREYAFPQDRERQHIKDIEYEERQISDFLNN"
     gene            11064..11375
                     /locus_tag="ACFJAS_001644"
     CDS             11064..11375
                     /locus_tag="ACFJAS_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982840.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            11389..11592
                     /locus_tag="ACFJAS_001645"
     CDS             11389..11592
                     /locus_tag="ACFJAS_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982841.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT         2880 a         2753 c         3275 g         2756 t
ORIGIN      
        1 aatgttaagc agaaacgatt gtctcaaaaa aagcaacttt ggataaatac aatcaagaat
       61 tggctgaact aggtgaaaat gaaaagaaaa aagaagaact agaaaaaaag taaaacagca
      121 agaaaacaaa attcagatca gaaaaaggca catagaacgt ctaaaagaag acatagaaaa
      181 ggttgcggta ctcgaagaag aaatcaaaag atctgaaaag ttcttcgatg aaaacggttg
      241 gcttaaagac agcattatcg acgacaaaac aaaaaacgcc gtcaacctca aaatgagcaa
      301 cggcgcgcgc tggacggtta ccaatgattc aatgctgaaa gagttggatt tgtcggaaga
      361 cgcgcaggtc gaatttagcg acaacaataa attcgtcaaa gtgtccgtga gcaagctcaa
      421 gggcgatggc ggcgtgttca aaatgtatgg cgacatcgtc aaaggagaat cggacaagct
      481 gattacccga aaaggcagcg aagggacgca catcatcgaa tatatggacg atgccaaggc
      541 aaaaacgacg ggaagggaat atctgaaact ggtcgaaaac aagggcaatc aagaagacaa
      601 taaagcatca aataaagcat catacaaatt gaatgtccgc tgtacggaac agggagggtg
      661 gtgttttgcc ttgggtgagt cgggtgcttc caaaaaggtc aatatttcca cagacggtaa
      721 gcgggacttc tacctctacc cggataccct gacgccgggc gcatcgagca gcgtcctttt
      781 cggcgaggcg ttgtatcagt tgaacgcggt ttccgatgaa acgctggtgc agcgtatggg
      841 cgaaatccac gccgacggaa cgccgcagga agacaacaac gtttggatca aacgcgtcgg
      901 cggaaaattc tccggcagcc gcagcgatta ccgcgtgggc ggttacggca accgctattg
      961 gggctttgcc ggcggcttca acaggacggg gttcggcgat aaatggattc attacaaagg
     1021 gctgatgctc cgccacctcc aatcgtccta cgcttccgaa gactacgtcg gcagcggcaa
     1081 aatcttacgg cagggcggca ggtgtttatt ccgcctggct caaccgggaa agcagggctt
     1141 attatgattt ggtcgccaac atcgcccgat ataagggcag ctacgggctg accaattacg
     1201 ccggcaaacg ggtcgaatcg gacgaggcgc gcctgaatgc ctatatgctt tccgccgaag
     1261 ccggtaggcg gatggaaaaa caagacggcg gcaaaaacct atcggtggca gccggaagtg
     1321 cagttgtctt actggtttac gcgcggctac ggctttccgc tgtcaaacgg gctgtctgcc
     1381 gagacggaca acttccgcag cctgatgggg cggttcggct ttcgggcggg cgtggacggt
     1441 ttggacggcg gcaggctgaa catctacggc aagctgatgt acaaacgcga gtttatcggc
     1501 acgatccgcc acaggttcaa cggctctgcc gtggaggaat tcaaacaccg gggcggctgg
     1561 ttggaatacg gcttgggcgt ggtgcgccgc aatgccggaa acggacggca gctttatttt
     1621 gaggcgcaaa ggtcttcgat gcacacgatg cgccagaatt ggcaggtcaa tatgggcgtg
     1681 cgcagtatgt tctgatgcgc ggatacgcct gaaaacaatc cggaacggtc agacgcgttc
     1741 cggattgttt ttttgtcggg gcggtttgct gccatatcaa aacaggcggg cgatgccgtc
     1801 tgaaacttcg tcattcccgc gaaagcggga atctagaacc tcaaactttc agataatctt
     1861 tgaatattgc tgttgctcaa tggtttggat tcccgcaacc tttcgtcatt cccgtgaaaa
     1921 cgggaatcta gaatctctaa agcttcagct aacctttgaa tattgccgct ttccaatggt
     1981 ctagattccc gcctgcacgg gaatgacgat ttggaaatta cccgaaaccc aaaaacaact
     2041 gaaaccgaac aggccggatt cccgctacgc gggaatgacg gggggcggat gccgtctgaa
     2101 gcggtgggct gaagcccact ctactattat ttttgcttcc ataccgcttg gcggcaatgt
     2161 gtccggaatg gagcggttta tccgaaaaag cccattttga cttggatgag tttctccagt
     2221 tcgttcagga tgcgccggcg cgagacgaaa aagatgatgt ggtcaccgtc ttggatgacg
     2281 gtttcggtat ggtgtcccat aatggtttcg ccggttccgg cgcggacgac ggcggcaatg
     2341 tggcagcctt cgggccattt gatgccgctg atgcgcctgc cgatgatggc ggaagttttt
     2401 ttgtcgccgt gcgcgacgac ttcgatggct tccgccgtgc cgcgccggat ggggtggacg
     2461 gcaacgatgt cgccgcgccg gatgtgggcg agtatcgagc cgatggtgat gaggtggggg
     2521 gagacgacga tgtcgatttt gttgccttcg agcaaatcga cgtagcttga gcggttgacg
     2581 atgccgatga cgcgcttcgc gccgaggttt ttcgccaaaa gggcggacat aatgttgctt
     2641 tcgtcgtcgt tggtcagggc gcagaatacg tcgatttcgt cgatgtattc gttgtcgagc
     2701 agggtttcgt cggttgccga accttgcagg acgagggtgt tgtcgaggtt ttcggctatc
     2761 cattcggcac ggcgcggccg gcattcgatg attttgacgt tgtatgcgtg ttcgagctgc
     2821 ttggcgaggc ggtagccgat gttgccgccg ccggcaatca tgatgcggcg ggtgctggtt
     2881 tctttggggc gcaattcggg tatgaccgcc ccgatgtttt cggcggcggc ggcaaacagg
     2941 atttcgtcgc cttcgatgat gacggtttgc ggcgcgggga cgatgaggcg gttgttgcgg
     3001 taaacggcgc agatttggca gtcggccccg tcgggcaaat cttgggcgat gtcggcaatg
     3061 ctgcgtccga caagcagccc gccgcgccgc gcctgtatga tgaccatccg cacgcggtcg
     3121 tctgcaaaac gtaaaacctg caatgcgccc gggcagtcta tcaggccggc aagctgttcg
     3181 gtaacgagct gttcggggct gatggtttcg gttatgccga atatggaaag gctgccgttt
     3241 tcgttgtttt cgagcttggg gctgaggtat tcgaggtatt cgctggaacg gacgcgcgcg
     3301 atgcggccgg ggatgttgaa caggtcggcg gcaactttgc aggcgacgat gttggtttcg
     3361 tcgctgcggg agagcgcgag cagcaagtcg gcatcttccg cgccggcgcg ttctaatgtg
     3421 aagggggatg cgccgttgcc gaaaacggtt tggacatcga ggcggctgcc tgtttcctgc
     3481 aatgcttttt cgtcgatgtc gataacggtt acgtcgttgt tggtgatggc ggcaaggttt
     3541 tgcgcgacgg tagaacctac ctgcccgttg cctaaaatga ggattttcac ggtatgggtc
     3601 gccgggtgat gaaatggtgg attgtaccgc aaccggcggc ggaaatgccg tctgaaggca
     3661 gattttgcgt tcagacggca ttgcgcttca agattcgacg gggatgatgc cgattttcgc
     3721 ctgccatttg cgcggggcgg tggcgtggat ggattcgcct ttgctgtcca cggcgacggt
     3781 tacgggcata tctttgactt caaattcgta aacggcttcc atacccaatt cggggaacgc
     3841 caagactttg gaagatttga tggcttttgc cacgaggtat gccgcgccgc cgactgccat
     3901 gaggtacacg gctttgttgt cggcgatggc ttcgcaggtg gccgcgccgc gctcggattt
     3961 gccgatcatg cccaagaggc cggtttgttt gagcatttgg cgggtaaatt tgtccatgcg
     4021 ggtggctgtg gtcggacctg cgggaccgac gacttcatcg ccgaccggat cgacggggcc
     4081 gacgtagtaa atcaggcggt tggtgaaatc gacgggcaac tcctcgcctt tgtcgagcat
     4141 attgacgagg cgtttgtgcg cggcatcgcg gccggtgagg attttgccgt tcaacagcaa
     4201 tacgtcgccg gttttccagc ttgccacttc ttctttggtc agcttatcga catcgacgcg
     4261 tttgccgttg tcggggctgt aagtcagatc gggtcagtct tcgacgcgcg gcggcgtgag
     4321 ttcgacagga cctgagccgt ccaattcaaa ttcgacgtgg cgggtggcgg cacagttggg
     4381 aatcatggca atcggtttgg aggcggcatg ggtcgggtaa tcgaggattt tcacgtccaa
     4441 cacggtggtc agaccgccca agccttgcgc gccgatgccc agcgcgttga ccttttcaaa
     4501 gagttcgagg cgcagggctt cggtggtgga caattccgcg ccggacgcgg ctttttcctg
     4561 caattcttgg atgtcgatgt ggctcatcag ggattctttc gccatcaaca cggctttttc
     4621 gggcgtgccg ccgatgccga tgcccaagat gccgggagga caccagcccg cgcccatcgt
     4681 cgggatggtt ttcaataccc aatcgacgat gttgtcggaa gggttgagca tagcgagttt
     4741 ggatttgttt tcagagccgc cgccttttgc cgcgcaggtt acttcgactt taccgcccgg
     4801 cacgatgctc atgtggatga cggcgggggt gttgtctttg gtgttttggc gtttgccggc
     4861 cggatcggcg aggacggaag cgcgcagggt gttgccttcc caagtgtagg cgcggcgtac
     4921 gccttcgtta accatctctt ccacgctcat gtccgcatcc cattgcacat ccataccgac
     4981 tttgaggaag acggttgcga tacctgtgtc ttggcagatg gggcggttgt tttcggcaca
     5041 catacggctg ttgaccaaaa tctgcgtcat cgcgtctttg gcggcgggat tttcttcctt
     5101 ctgccacgcc ttataaagcg cgtcgatgta gtcttttgga tggtagtagc tgatgaattg
     5161 gaaggcatcg cagatacttt gaataaagtc ttcttgcttg ataacggtca tgattgtgtt
     5221 ccttttcaag gttgtggcgg taagtgtctg ctgctgtttt aaacaacctt tttgaaggct
     5281 gttttttatc aggttgaaat agtgccagcg tttgtacggt gaagacaata cataattcta
     5341 atctcttgat tatcaatttg ttttaataaa atactgtttt attcggattt tgatatttgt
     5401 aatctttgcc tgttgctttg cggtagggag aaatcagcag gcagttcatt gtggaccggt
     5461 tgatgaacaa tgccgtctga aagggcttca gacggcattt tgttcggacg ggcgggctta
     5521 tttcgcagcc tgtccgccct ctttggcaaa atatttggcg tagatcttgt cgtattcgcc
     5581 gctttcgcgt actttttcca acgcatcgtt cagcattttg acggttgctt cgtcgccttt
     5641 gcgtaccgcg atgccgtagt gttcggtggt gaagtcgggc agggtaacga agtccattcc
     5701 tttggccggg ttgtttttca cataattggc gatgaccgcg ctgtcgctga ccacggaatc
     5761 caagccgccg ttttccagtt ctttgataat cagggggacg ttttcgaagc gcgcgatttt
     5821 cggattgtcg ttgcccaaga gtttggaaac ggagaaatcg cccgtgtggc cggtaaccac
     5881 gccgactttg ttcatctttt tcaaatcttc ggaagaagat acttttttgc ctttcggaac
     5941 gaggacgact tgggtgattt caaaatacgg gtcgctgaaa tccatagact gtttgcggtc
     6001 gtcggtaatg gttacgcccg acatcacaac gtccgcatcg ccgttgttca aggcggggaa
     6061 aaggctgtcc cacggctggt gtttgaattc gattttaaaa ttgcccgcct tcgccatcgc
     6121 gttcatcaaa tccacgtcga aaccttcgac attgcctttc gagtctaaag attcaaaggg
     6181 ggcaaactcg gcgttggaag ccacgcggta cactttgccg gggttggcgg caggcgcggc
     6241 ggcatctttg ccctgaccgc cgcaggcaga cagcgcgagt gcggaacagg caagggcggc
     6301 ggcaatccat tttttcatat tcatcaaaat tcctttcggt aatcaaaaag cctaaaaaat
     6361 cagtttaaca taaaattaac gttccttttc acggaaaaac gcataagtaa cgtcgtggaa
     6421 caaatccgta tgcgggctgt cctcatagct caattctgcc aaggcgtcct caaaagcaag
     6481 ctgaatcgac tcgaccgcga tttcggcggt gtctgcggga agggcgcgga tcagggcttt
     6541 gaaggcggtg gacaggacgt ggttctgcat acgcagcaca tcgtttgctt cttccagata
     6601 ttccaaacgc tgttcgatgc tgcccatgat gtatcctcgc gaatctgtta aaatgcgcca
     6661 ttatatcaaa ctgcgctttg ggcgcgggtt tgcccgcctg caaagattaa gaaccggaag
     6721 ccgaaaaaat ggatacaaac aatcaggata aagagccgaa ttgggaaaaa cgcctggaaa
     6781 acctgcgtca gacgcgccgt atgaataccg cgcctgccta tgtgccgctg accaaacctt
     6841 ctgaagtcgg aaaggaagag cccataccga aattggacga tgcaaaccga gaccgtgtgt
     6901 taaaggcgta tctgaagaaa tggcaggaag aacacaacag cgccgttgcc gattgcggcg
     6961 aggaagtcga aaccgacccg atgatcgtcc ttcaggaaaa ctggctcaac gcgcaggctt
     7021 ccctgcaaat gccggtttcg gaaaaacaca tcgaatcgcg gcggagaatc cggttcgatg
     7081 ccaaaaaaga cagtgccgaa tttgaaaccg cacagattga gggcggggaa aacgccgatg
     7141 cggcagaatc cggtacggcg gcagacgggg ttgcggaaaa cggcgaaggc ggtgcggaag
     7201 aggcgttgat gcccgtccaa atcaatatcc tgaacccgaa agccgtcaac cggcgcgaag
     7261 tgttctgcct gtccgaacag gagctgaccg aacggttgat caaacgcctg cgcccgcatc
     7321 tgaccgatac cgtcaacggc atgatccgta cggcggtgca gaagcagatg gcgctcttta
     7381 cttatcagct ccagcaaatg ctgcacgagc aggcgggtgc ggtagtcgaa gacgtgttgg
     7441 aacacgatgt ccgtaaaatc ctcaacgaca tcaagtacga attaaaatac aaacgctgat
     7501 tgccatgccg tctgaaagcc gtgttcagac ggcattttcc tgcccgtccg acttgaaagc
     7561 ctcatggaac aggcgcaaaa tattaataaa ttaaacaacg ggtgcacgcc ccgttgccga
     7621 acggacaaaa ccgaggaatc accgatgagc aaaaaacttc atccgcaaac gctcgccata
     7681 cgcggcggca aagaacaaac cggataccgc gaacacaatc aggcattgtt cctgaccagc
     7741 agcttcatgt gggacaacgc ccaacacgct gccgatttgt tttcaaaaaa aatcaaaggc
     7801 ttcacttata cccgtaccgc caacccgacc atcgcggcct ttgaaaaacg catagccgct
     7861 ttggaaggcg cggaacgcgc ggtcgccact tcgaccggca tgtccgcgat tcaggcggcg
     7921 tttttcacct tcctgcaggc gggcggccat gtggtttcca gccgcagcct gttcggcacg
     7981 accgtcggct ttatcaataa catcgttacc aaattcggca tcggagtgag ccgcgtgtcg
     8041 ccgactgata taaatgaatg gaaagccgcc gtcaaagcca acaccaaact gctgtttttg
     8101 gaaacgccgt ccaacccctt gggcgaagtg gccgacttgg aagccttggc ggaattggcg
     8161 cacggcatcg gcgcgctctt ggtggtggac aacagcctgc tgtcgcccgt cggctcgcag
     8221 cctttgaaac acggcgcgga tatttccgtt tcctccgcca ccaaagccat agacggacac
     8281 gggcgcgtga tgggcggcgt gttggcgggt tcggaagaac tgatggcgca ggtcgccgtt
     8341 tactgcaact cttgcggttt ggcgatgtcg ccgtttaacg cgtggcagtt gttgagcggc
     8401 gtggaaaccc tgtcgctgcg tatggaaaaa cagtttgaca acgccttgag aatcgcgcaa
     8461 tggctgcaag agcagccgca ggttcaagca gtgtactaca ccggattgcc cgaccatccg
     8521 caggcggagc ttatccgcaa acagcaaaac ggcggcggca tcgtcatcgg ttttgaagtc
     8581 gccgaccaag cagccgcgtg gaaagtcgtg gacggcgtgg aactcttttc ccgtaccgcc
     8641 aacctcggcg acgtgcgctc gaccatcacc aatccgtgga caaccacgca cggcaggatg
     8701 cagcccgaag aaaaactcgc cgccggtatc cgccccggac tggtgcgcct gtcggtcggt
     8761 ttggaatgtg ttggcgattt gattgacgat ttgaaacagg cactcgcgcg ctgattgcgt
     8821 gtttcagacg gcatcggcgg aaaaatgcgg cagatgccgt ctgaaaccct tattgcgtat
     8881 ggcatacaca tcaacaatag gaaaaaatat ggatgaagcg gtttttgccg attgggcgtt
     8941 gaaaatctgc ctgaccggtt tgattatttt tttgggtttt atcgtttgga atttgggcaa
     9001 ggagtccaaa gcgggcaaat tcggcatcgc cgttttgttt ttggttttgg gcttgggtgt
     9061 gttcggtttt gtgttcaaag agttattgat taagtttttg gtattgccca aataggtttg
     9121 ccgtctgtcg aaatgccgtc tgaaatcata aaaagtaatc tgcaccctaa aagtttgatt
     9181 taaccgccaa ctgattaagg tgcgggtttt tatgacaaaa taaaaatcat ctttccgcaa
     9241 tcattcccac ggatgcgaaa gcctgtcttt ttgaatcccg gataatttta catcgtcatt
     9301 cccgcgcagg cgggaatcta gtccgttcgg tttcagtcat ttacgataaa ttcctgttgc
     9361 ctttcatttc cggattccca ctttcgcggg aatgacggag gagcggataa attctcgcaa
     9421 tctaaaacct tatatattgc gctccataag gggcgcatat ttttaatcct tgtaaagccc
     9481 cttaactcta gtcagtgaag gggcttaatc atgtttattt tctatactgt caatcccgaa
     9541 cacgtatatt tccccaaagc atacatcatg aaagtgttca aggataaagg ctacgaatcc
     9601 caatgcatca caacagtttc tttttatatc tgcaatccga ctttgaagca aaagaccgaa
     9661 aacgaagcgt atgaatacgg cagattgttt gttaaagaac tgatgcataa ggaatgcaac
     9721 cgtgaaagcc tttgaagata aagccttggc ggtgtccgcg gacatggcgg acgcgttagc
     9781 ggacggcggg gctgcggcaa ccgccgtagg ctgcccccct aggctaatag ggggggagca
     9841 aaataaaacc ccaaatccga agggtgcgga aaaaacagaa aaccaagaat ttcaattcga
     9901 gtatttcagt cattttgtat cggacggcaa aggcagattt atagaaatac cgttaaggcg
     9961 tggcagggat gacggagcgt tcatagacca aatcactttc acgatacatg aaaattccat
    10021 gacgaaagta accggaaagg gattggtatc agatacggaa ttcgttgtcc ggtatagcga
    10081 gctgcttgaa gaaatcttgg gattcggcat taccaaaaaa ttaccgttta aaggaaagtt
    10141 tttctatcaa agctgttatc aattcgggcc cgataacgtc gaatacggaa aggttcatta
    10201 cggcggccaa tgtgaaacga tgttggttga attgaacggt acaggctgca tggctgcttt
    10261 acccggttgg gaaaaccgat tgtatgaatt tttaagtaaa tgcgtccgac ccaaaataac
    10321 ccgtatcgat gtcgcccatg attttttcaa cggagagtac acacccgatc aggcaatgtt
    10381 agaccacgat aacggacatt acgacgtaca caatatgcgg ccaaaaagcg aatgtcgcgg
    10441 cacggcatgg cgcaatgaag acggcagtgg aaaaaccttt tacatcggca agcgcggaaa
    10501 ttcaaaattt acccgtgttt acgagaaagg cagacagctt ggagatgttg atagcccttg
    10561 ggtcaggttt gagggggata tagaaatacc cttggatgtc ctactttatt cggggtcgta
    10621 tctaggcggc gcatatccga tttgcaagga gatttttaag acagaagcca agcgcatgga
    10681 agtaaaagtt aaaaacgtta atctgatatt cgacgttaag ctttttcatg caaggaatca
    10741 agtcggcaag atggttaatt tcctccgcga tatagggtgg gacgatagca ggatagttga
    10801 tgagctggtg aaaggcgttg aaggttatcc aaaaggttta cagcctgagc aatacgattg
    10861 caaaaaccaa acgcagaaat tccagtatat ccacgaagaa cagaaagcca ttaacgcatt
    10921 gaacattgaa acactgtttg atgatttgat tgaagagaga gaatacgcat ttcctcaaga
    10981 cagggaacgg caacacatca aggatattga gtacgaggaa cgtcaaatat ccgatttttt
    11041 aaacaactga aggaaattga aaaatgtttg aaacaagcca agtaaccaca tataccgcca
    11101 ctttgttggg cgcaaaaaaa ttcaaaggcg aaatcgacgg taacaaaatc gattcatgca
    11161 ccgtcttggt agccagcccc atgccgtcaa acggcaatgc cgtgggcttt acctcggaaa
    11221 gcatgaagtt tggtgacagc cataattttg aaaaattgaa aaaccttaaa ttcccctgtg
    11281 cggttgatct aaccgttgcc atggaatcaa caggaaaagg cttagtgcag aaattgttgg
    11341 atttccaagt aaagggcgca gcacctaaag cctaagaaag gctgaatcat gagcagatac
    11401 caacagaaat ttatcgtaca ggaattggaa aatcacgaat tcatctatcc cgattcgttt
    11461 ggcgatattg gatttacgtc gaacatcaaa tcagccggta aatatgacag ctatgaagac
    11521 gcttttagct cggcattgga agagataggc ggggaatttg taattttcgg attctacgaa
    11581 aaagaagatt aagtttaaga ggctcggcag gcggtctcta aaaccttcac aaagcccgca
    11641 aacacatttt tttaaaaatt tcgt
//
LOCUS       DAXFZR010000068        11659 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000068 DAXFZR010000000
VERSION     DAXFZR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.068"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(265..1344)
                     /gene="apbC"
                     /locus_tag="ACFJAS_001646"
     CDS             complement(265..1344)
                     /gene="apbC"
                     /locus_tag="ACFJAS_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC3982842.1"
                     /translation="MNIQNIRTLLETVAIPDTGRTIGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPETGGAHIHLGIDAEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTATANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPARLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1563..1638
                     /locus_tag="ACFJAS_001647"
     tRNA            1563..1638
                     /locus_tag="ACFJAS_001647"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1596..1598,aa:Lys,seq:ttt)
     gene            1711..2220
                     /locus_tag="ACFJAS_001648"
     CDS             1711..2220
                     /locus_tag="ACFJAS_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC3982843.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            2561..3001
                     /gene="nadR"
                     /locus_tag="ACFJAS_001649"
     CDS             2561..3001
                     /gene="nadR"
                     /locus_tag="ACFJAS_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC3982844.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3116..3616
                     /gene="hpaC"
                     /locus_tag="ACFJAS_001650"
     CDS             3116..3616
                     /gene="hpaC"
                     /locus_tag="ACFJAS_001650"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC3982845.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            3632..4327
                     /gene="murU"
                     /locus_tag="ACFJAS_001651"
     CDS             3632..4327
                     /gene="murU"
                     /locus_tag="ACFJAS_001651"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC3982846.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(4361..4791)
                     /locus_tag="ACFJAS_001652"
                     /pseudo
     CDS             complement(4361..4791)
                     /locus_tag="ACFJAS_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            4966..6642
                     /locus_tag="ACFJAS_001653"
     CDS             4966..6642
                     /locus_tag="ACFJAS_001653"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC3982847.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6756..7847
                     /gene="ychF"
                     /locus_tag="ACFJAS_001654"
     CDS             6756..7847
                     /gene="ychF"
                     /locus_tag="ACFJAS_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC3982848.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            7885..8124
                     /locus_tag="ACFJAS_001655"
     CDS             7885..8124
                     /locus_tag="ACFJAS_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982849.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            8121..9995
                     /locus_tag="ACFJAS_001656"
     CDS             8121..9995
                     /locus_tag="ACFJAS_001656"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC3982850.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGRFFLKTFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFL
                     LWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVAR
                     KSLRVQFGFLAALCALSLAASFMPSALDKYYLPHLRACEMLVGSLTAVRMRYRQQRNP
                     AVGKRYAAVGALFSACILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLK
                     KFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYH
                     CIEKPFKKWKGSFAQSVLWIYALPMLVLGAGSFFAMRLPFMAQYDRLGLTRSNTSCHN
                     NTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYA
                     SRVFQNWAACRAVYRYAEEHLPRYPKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRML
                     HKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGCRQTLRPDDESTLKANARIRELA
                     AKYPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH
                     "
     gene            10057..11352
                     /gene="tyrS"
                     /locus_tag="ACFJAS_001657"
     CDS             10057..11352
                     /gene="tyrS"
                     /locus_tag="ACFJAS_001657"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC3982851.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERADDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2582 a         3192 c         3243 g         2642 t
ORIGIN      
        1 aacagctgca aatctaagtt gcctttcaaa tcgcctgcaa ccgaaaaatc tgccgaaacc
       61 cactccctcc cccgtggggg agggctgggg agagggcatt ctccgaatgg tggcaatctt
      121 tcctaatacc ttcgccgccc caatacaagc cttgcggctt gttgccctct ctccagccct
      181 ctcccacggg gagagaggac tatgaagccc gccggcaggc gcggggcttg ggacggcatt
      241 gctgttgcgg tttcggacgc ggctttattc gacgacaatt ttagggaacc ggctgctgaa
      301 gtctttgcct ttgtcggcaa tgcccagggc gatttggaat gcggcatcgg tgtagattcg
      361 ggcgatggcg gggtgttcgt cgaacagtcg cgccggtgtg ccgccgtcca tggcttcgcg
      421 tacgggcagg cttaggggaa gctgtccgag cagggggacg ttgaggcgtg ccgccaaatc
      481 tttgccgccg tccgtgccga acagtgcttc gctatgcccg cagttggagc agatatggac
      541 ggacatgttt tccaatacgc ccaaaatggg aatgttgacc ttgcggaaca tatccacggc
      601 tttgcgcgcg tctatcagcg cgatgtcctg cggcgtggtt acgatgacgg acccggttac
      661 ggggatgcgt tgggacagcg tgagttggat gtcgcccgtg ccggggggca ggtcgatgaa
      721 aagatagtcc acttcgtccc actcgctttg gaacatcagc tgctgcaagg cttggctgac
      781 catcggcccg cgccagacga cggcttggcc ggtatcgacg agaaagccga tggacatgac
      841 ctgtatgccg tctgaagatt cgacgggaat gagtttttgg tttttctgat cgggtttgcg
      901 gtcgtgtacg cccagcatcg tgggctggct cgggccgtaa aggtcggcat cgagtacgcc
      961 gacgcgcgcg cccatccgcg ccattgcggc ggcaaggttg gcagtggcgg ttgatttgcc
     1021 cacgccgcct tttcccgatg cgacggcgat gatgtttttc acgcctttga tggtggcgac
     1081 gccgggccgg actttgtgtg tgccgatttc ggcgtctatg cccaagtgga tgtgcgcgcc
     1141 gcctgtttcg ggcatcaggg tttcttgtac ggcatcggcg agggcggcgg cgatgtgcgc
     1201 gacagggaag ccgaaatgca gggcgatgtg gatgccgtct gaacgctgtc cgaccgagcg
     1261 gacggccttt tcgccgccaa tcgtgcggcc ggtgtccggg atggcgacgg tttcgaggag
     1321 ggtgcggatg ttttgtatat tcatgatgtg tgcctctgtt cgcaagtcgt gtgaggggaa
     1381 gatagacgga aagtgtacat gatttcgggc agtttgcttt tttcttgtgc gaacttttac
     1441 acggtatgcg gcggattgtg aaaatccgtt gccaaaatat cccgtgcaaa cagcagtctg
     1501 caattaagcc tgtaaaattt gcttgacggc ggggaacggg gctgtataat gcacggctta
     1561 tcgggtcgtt agctcagccg gtagagcagc ggacttttaa tccgttggtc gagcgttcga
     1621 atcgctcacg acccaccaga taaccggaag ccaagtttcg agacttggct tttttgtttg
     1681 cctgccgggg cgtaccgttt gcgggggtgt atgaaaaaga aactgctttc gggcatcaaa
     1741 tttgccgttc ggacggcatt ggtgtttttg ctggtgtcgc tgtttttgga ttgggtacgc
     1801 aagcccgacg aacctgccgg ggcggcaggg cggcctttga ccctgctgtc ggggcagcgg
     1861 ctgactttgg gacagtttag ccgggatagg acggtgctgg tgtatttttg gggaagctgg
     1921 tgcggcgtgt gccgttatca gtcgccgata atcgatgatt tggcggcgga cggcgtgccg
     1981 gtcgtcggtg tggcggtacg ttccggcagc gcgtccgaag tggcggcata tatggcaaaa
     2041 cggggcttgg gctttccgac tgtcagcgat gaggacgggg gtttggcaag gtcttggcgg
     2101 attgccgcaa cgcctgccgt cgttttggtc aaaaatggga aaatggtccg ctatacgacg
     2161 ggaatcagca gttattgggg cttgcgcgca cgaatttttc aggcggattt tttcggttaa
     2221 cttttgtttt tgtcaaactt tccgctatgt agagaatcaa acggcatatg ccttgcgcgg
     2281 cggatgcggc ggcttttgtt ttttgggaaa gagccggcgc gcctgccgat gtaatccctt
     2341 gttgtgattg tgggaaaaat agattaaaat ataactatta aaatattttc agataggatt
     2401 atcggaatta aagtctttta tacccggtcg tccgatgagg tttatagcgt attgttgcta
     2461 tatgttcgtt ttgttatata acggttgcat caaaaattac gcccacaggc tttcccgacg
     2521 gtttgaaagt ttgattttcg ataacttgga gacttaaaca atgcctaccc aatcaaaaca
     2581 tgcgtctatc aatatcggtc tgatacaggc aagggaagcc ctgatgaccc aattcaggcc
     2641 tattctgaat caggcgaata ttaccgatca gcaatggcgg attatccgtc ttttggcgga
     2701 aaacggcaca ttggattttc aggatttggc gaatcaggcg tgcattttgc gccccagcct
     2761 gaccggtatc ctgacccgcc ttgaaaaagc ggggttggtc gtccgcctga aaccttccaa
     2821 cgaccaacgg cgcgtttatc tgaagctgac ttccgagggc gagaagctgt atgaggaaat
     2881 cggcgaagaa gtggacgagc gttacgacgc tatcgaggaa gtgctgggcc gcgagaaaat
     2941 gctgctgctt aaagacctgt tggcagaact tgccaaaatc gaggatgcgt tgaactcgta
     3001 atacgccgta atgcgcggaa acgtccgacc gacggctttt tgaatcagaa ctgctgcaca
     3061 tgggggatgc cttgtgtgca gcattcttat ataggggacg gtttaaaggg gaaaaatggc
     3121 ggatttgcag aaaacttttc aaacttcgtt ccgtgatgcg atggcatcct gtgcggcagg
     3181 cgttcatgtc atcacgacag acggtgcggc agggcgttac ggcattacaa tgacggcggt
     3241 tgcgccggtt accgacgagc cgccgaccgt gatgctgtgc ataaaccgga gtgcgcgaat
     3301 cattccgatc ctgtcggaaa acggcagcct ctgcatcaat atgctggcgg acgaacatca
     3361 ggatgttgcc gaacattttg ccgggctgac cggcctgtcg cccgaagagc ggtttgccta
     3421 ccatatttgg catcgcggca aaacgggaca acttgaaata gaaggcgcgt tggcgcacct
     3481 gcacgggcat attgtcggca aacatgaaat cggcacgcat tttgtgtttt acgtcaggct
     3541 cgacgaaatc aaaaactgcg ggtgcaaacg ccccgcgctg ctgtatttca gacggcagtt
     3601 taggccttta gactgatatt cggacagata tatgaaagcg atgatactgg cggcaggacg
     3661 cggcgagcgt atgcgccctt tgaccgacac cactccgaag ccgctgctcg atgtggcggg
     3721 taagcctcta atcggttggc acttgtgccg tctgaagcag gcggggttta ccgaaatcgt
     3781 catcaaccac gcttggctgg gtcggcagat agaaaatgct ttgggcgacg gctcggctta
     3841 tggcgtgaac atcgcctatt cgcccgaacc cgcaggcggt ttggaaacgg caggcggcat
     3901 cgcgcaggca ttgccgctgt tgggtgggca gccgtttttg gtggctaacg gcgacgtgct
     3961 gaccgacatc gattttaccg ccgcgtttca gacggcatcg tccctgcccg ggcacatttc
     4021 cgcacatttg tggctggtgg gaaatccccc gcacaacccc gacggcgatt tttccctgct
     4081 gcccgacggc agcgtgcggc cggaagtatc cggcggcaac ggactgacat tcagcggcgt
     4141 gggtatttac cgtcctgaaa tgtttgacgg aatcgaagcg ggcagtgtgg caaaactcgc
     4201 gcccgtattg ctgaacgaaa tgcggcaaaa ccgcgtgagc ggtcagaagc atacgggcct
     4261 gtggctggat gtcggcacgg tatgccgtct gaaggaagcg caagcccttg cagcggcttg
     4321 gaagtgaaaa ctcggtttca ggcggtatgg cggattcggt ttaacgtttc agtgccaacg
     4381 tcaacacgcc gccgtaacca gccccaagtc tatccattcc tgcgtgttcg ggcgttcgtc
     4441 taagaaaatc accgccatca gcgcgaccaa gaccaggctg aatttgtcga cgggcgcgac
     4501 ttgcggggct ttgcccagtt gcagggcttt gaagtaggcg agccaagatg cgccggtggc
     4561 gagtccggac aaaaccggaa acgtccggtt gcgccccgtg aagccgttta cgccctgcca
     4621 tttgccggtg taggtcaaaa acaataccaa ggcggcgagg atgaccaagg tgcggataaa
     4681 ggcggcgaaa tccgaatcta tgccctgcaa gcccattttg gcgaaaacgg cggtcaatga
     4741 ggcgaagcct gccgatgcca atgcccaaaa cagccatgcg ttgctgccca tgttttctcc
     4801 tttgattgtg aacaatatga acggtatttt tgttgctgcg tcaaaaattt cactgcgggt
     4861 ttggtgcgga taacgttata atatgcctga tattattttc aatccacctg tttgtcgcct
     4921 gatgctttca gacggcatgt ccctcctcat ttctaaagga aaatcatgag cttcaaaacc
     4981 gatgccgaaa ccgcccaatc ctccaccatg cgcccgattg gcgaaattgc cgccaagctg
     5041 ggtttgaacg ttgacaacat tgagccttac ggtcattaca aagccaaaat caatcctgcc
     5101 gaagcgttca agctgccgca aaaacaaggc aggctgattt tggttaccgc catcaacccg
     5161 actccggcgg gcgaaggcaa aaccaccgta accatcggtt tggcggacgc attgcgccat
     5221 atcggcaaag actctgtgat tgctttgcgc gagccttctt tgggtccggt gttcggcgtg
     5281 aaaggcggcg cggcaggcgg cggctacgcg caagttttgc cgatggaaga catcaacctg
     5341 cacttcaccg gcgacttcca cgccatcggt gcggcgaata acctcctcgc cgccatgctc
     5401 gacaaccata tctaccaagg taacgagttg aacatcgacc ccaaacgcgt gctgtggcgg
     5461 cgcgtggtcg atatgaacga ccgccagttg cgcaacatca tcgacggtat gggcaagcct
     5521 gttgacggcg tgatgcgtcc cgacggcttc gacatcaccg tcgcctccga agtgatggcg
     5581 gtattctgcc ttgccaaaga catcagcgat ttgaaagagc gttttggcaa tattctcgtc
     5641 gcctacgcca aagacggcag ccccgtttac gccaaagatt tgaaggcaca cggcgcgatg
     5701 gcggcattgc taaaagatgc gattaagccc aatttggtgc aaaccatcga aggcactccg
     5761 gcctttgtac acggcggccc gttcgccaac atcgcccacg gctgcaactc cgttaccgca
     5821 acccgtctgg cgaaacacct tgccgattac gccgtaaccg aagcaggctt cggcgcggac
     5881 ttgggtgcgg aaaaattctg cgacatcaaa tgccgccttg ccggtttgaa acctgatgcg
     5941 gcagtcgtcg tggcgactgt ccgcgccctg aaatacaacg gcggcgtgga acgcgccaac
     6001 cttggtgaag aaaacctcga agccttggca aaaggtttgc ccaacctgtt gaaacacatt
     6061 tccaacctga aaaacgtatt cggactgccc gtcgtcgttg cgctcaaccg cttcgtgtcc
     6121 gactccgatg ccgagttggc gatgattgaa aaagcctgtg ccgaacacgg cgttgaagtt
     6181 tccctgaccg aagtgtgggg caaaggcggc gcgggcggcg cggatttggc gcgcaaagtc
     6241 gtcaatgcca tcgacaacca acctaataac ttcggtttcg cctacgatgt cgagttgggc
     6301 atcaaagaca aaatccgtgc gattgcccaa aaagtgtacg gcgcggaaga tgtcgatttc
     6361 agcgcggaag cgtctgccga aatcgcctcg ctggaaaaac tgggcttgga caaaatgccg
     6421 atctgcatgg cgaaaaccca atattcattg agcgacaacg ccaaactctt gggctgcccc
     6481 gaaggcttcc gcatcaccgt acgcggtatc actgtttccg ccggcgcggg cttcatcgtt
     6541 gcgttgtgcg gcaatatgat gaaaatgccg ggcctgccga aagttccggc tgccgagaaa
     6601 atcgatgtgg acgaacacgg cgtgattcac ggcttgttct gaacggtttt tgaaaccgga
     6661 tgccgtctga agccgtttca gacggcattt tttcggaacg cgggcggcgg tatgctataa
     6721 ttctccgtta aatttctcta ttttcaggaa aaaccatgag tttgaaatgc ggcatcgtcg
     6781 gtttgcccaa cgtcggcaaa tccacccttt ttaacgcgct gacccaatcg ggcatcgaag
     6841 cggcaaacta tcccttctgc accatcgaac ccaacgtcgg catcgtcgaa gtgcccgacc
     6901 cgcgtatggc ggagctggcg aaaatcgtca atccgcaaaa aatgcagccc gccatcgtcg
     6961 agtttgtcga tattgccggc ttggttgcgg gcgcgagcaa aggcgaaggc ttgggcaacc
     7021 ggttccttgc caacatccgt gaaaccgatg ccatcgtcaa cgtcgtgcgc tgctttgacg
     7081 acgacaacat cgtccacgtt tccggcaaag tcgatccgat tgccgacatc gaaaccatcg
     7141 gcaccgaatt ggcgcttgcc gacttggcaa gtgtggaaaa agccatcgtc cgcgaagaaa
     7201 aacgcgcccg atcaggcgac aaagacgcgc aaaaactagt cgatttgtgc aaaaaactgc
     7261 tgccgcatct ggacgaaggc aaacccgtgc gttccttcgg tttggacgcg gaagaacgcg
     7321 cgctgctgaa gccgctgttc ctgctgaccg ccaaacctgc gatgtatgtg ggaaacgttg
     7381 ccgaagacgg ttttgaaaac aacccgcacc tcgaccgcct gaaagaattg gcggcaaaag
     7441 aaaacgcccc cgtcgttgcc gtctgcgccg cgatggagag cgaaatcgcc gaattggaag
     7501 acggtgaaaa agccgaattt ctcgccgaaa tgggcttgga agaaccgggc ttgaaccgcc
     7561 tcatccgcgc gggttacgac cttttgggac tgcaaaccta cttcaccgcc ggcgtgaaag
     7621 aagtccgcgc gtggacgata cacaaaggcg acaccgcgcc gcaagccgcc ggcgtgatcc
     7681 atacggattt tgaacgcggc ttcatccgcg cccaagtgat tgcctacgat gattttgtct
     7741 cgctcggcgg cgaagccaaa gccaaagaag ccggcaaaat gcgcgtggaa ggcaaggaat
     7801 acgtcgtgca ggacggcgac gtgatgcact ttttgtttaa cgtgtaaccc aaatgcggca
     7861 ggttttcaga cggctttgcc ggaaatgccg tctgaagccg gttttggtgg ttttcgacgt
     7921 tcccataccg ccggaatgca gccgcatcaa aataaaatcc cgcccgcatt tccgatttgc
     7981 cctccccgat tcctgcaaaa caaaccgcct gccctgccgt tacgggaagc cgtccggtat
     8041 tccgaatatc ccgaaccccg atacaaaatg acctttcaga cggcatttgc acagcccgcc
     8101 gcgtttcaag taaaaacatt atgagccaag ccttacccta ccgcccggac atcgacacat
     8161 tgcgcgccgc cgccgtcttg tccgtcatcg tgttccatat cgaaaaggat tggctgccgg
     8221 gcgggtttct cggtgtcgat atattctttg tgatttcagg ctttttgatg acggcgatcc
     8281 tccttcgcga aatgtccggg gggcgtttct tcctcaagac attttatatc cgccgcatca
     8341 aacggatttt gcccgcattt ttcgccgtat tggcggcaac gctggcaggc ggcttctttt
     8401 tattcaccaa agatgatttc tttcttttgt ggaaatccgc gctgaccgcc ttgggtttcg
     8461 cctccaacct gtattttgca agggggaagg attatttcga tcccgcgcag gaagaaaagc
     8521 ccctgctgca catctggtct ttgtcggtcg aagaacaatt ttactttgtc tttccgatat
     8581 tgctgttgct tgtcgcccgc aaaagcctgc gcgtacagtt cggcttcctc gccgcattgt
     8641 gcgccttaag ccttgccgct tcctttatgc cttccgcgct cgataaatat tacctgcccc
     8701 acctgcgcgc ctgcgaaatg ctggtcggat cgctgaccgc cgtgcggatg cggtaccggc
     8761 aacagcggaa tcccgccgtc gggaaacggt atgccgccgt cggcgcattg ttttccgcgt
     8821 gcatactgtc cgcctgcctg tttgcctatt cggaacaaac cgcctatttc ccgggccccg
     8881 ccgctttgat tccctgtctg gctgttgccg cgctgattta tttcaaccat tacgaacacc
     8941 cgcttaaaaa atttttccaa tggaaaatca ccgttgccgc cggtttgatt tcctattcgc
     9001 tttatctgtg gcattggccg atattggcct ttatgcgcta tatcggcccg gacaacctgc
     9061 cgccttattc gccggcggca gcgatcgtcc tgaccctggc gttttccctg atttcttatc
     9121 actgcatcga aaagccgttt aaaaaatgga aaggctcgtt cgcacaatcc gttttatgga
     9181 tttatgcctt gcctatgctc gttttgggcg cgggctcgtt tttcgcgatg aggctgccgt
     9241 ttatggcgca atacgaccgc ttggggctga cgcgttccaa cacctcctgc cacaacaata
     9301 ccggcaaaca atgcctgtgg ggggatacgg aaaaacagcc ggaactgctg gttttgggcg
     9361 actcccacgc cgaccattac aaaacattct tcgatgccgt gggcaaaaaa gaaaaatggt
     9421 ccgccactat ggtttccgcc gacgcctgcg cctatgtgga aggctacgcg tcccgtgtgt
     9481 tccaaaactg ggccgcctgc cgcgccgttt accgctatgc cgaagaacac ctgccccggt
     9541 atccgaaagt ggttttggcg atgcgctggg gcagccagat gcccgaaaac agccgctccc
     9601 ttgcctacga tgccggtttt ttccaaaaat tcgaccgtat gctgcacaaa ctctcatccg
     9661 aaaaacaagc cgtttacctg atggcggaca acttggcttc gtcttacaac gtccagcgcg
     9721 cctatatctt gtcttcacgc ataccgggtt gccgccaaac actgcgcccg gacgacgaaa
     9781 gcaccctgaa agccaatgcc cgcatcaggg aattggcagc caaatacccc aacgtctata
     9841 ttattgatgc cgccgcctat atccccgcag atttccaaat cggcggattg ccggtttact
     9901 cggacaaaga ccacatcaac ccttacggcg gcacagaatt ggcgaagcgt ttttccgaaa
     9961 aacaaaggtt tctcgatacg cgccataacc attgattcgc ttaaatttgt tacaatcggc
    10021 ggtttgcaaa accgctaatt tttttgaaag agaccgatga gcgtcatcca agacctgcaa
    10081 tcgcgcggcc ttatcgcgca aaccaccgac atcgaagcct tagacgcttt gctgaacgaa
    10141 caaaaaatcg ccctttactg cggtttcgac ccgacagccg acagcctgca catcggacac
    10201 ctgctgcccg tattggcatt gcgccgcttc caacaggcgg ggcatacgcc gattgcactg
    10261 gtgggcggcg cgaccggtat gatcggcgac cccagcttca aagccgccga acgcagcttg
    10321 aattccgccg aaaccgttgc cggctgggta ggaagcatac gcagccaatt aacccctttc
    10381 ttgagctttg aaggcggaaa cgccgccatt atggcgaaca atgccgactg gttcggcagc
    10441 atgaactgcc tcgacttcct gcgcgacatc ggtaagcatt tctccgtcaa cgccatgctg
    10501 aacaaagaat ccgtcaaaca gcgcatcgac cgcgacggcg caggcatttc ctttaccgag
    10561 ttcgcctatt ccctgctgca aggctacgac ttcgccgagt tgaacaaacg ccacggcgcg
    10621 gttttggaaa tcggcggttc cgaccagtgg ggcaacatca ccgccggtat cgacctgacc
    10681 cgccgcctga accaaaaaca agtgttcggt ctgaccctgc ctttggttac caaatccgac
    10741 ggtaccaaat tcggcaaaac cgaaggcggc gcggtgtggc tgaacgcgaa aaaaacctcg
    10801 ccgtaccagt tctaccagtt ctggctgaaa gtcgccgatg ccgatgtgta taaattcctg
    10861 aaatacttta ccttcctgtc catcgaagaa atcggtgtca tcgaagccaa agacaaggca
    10921 agcggcagca agcccgaagc gcaacgcatc ctcgccgaag aaatgacccg cctgattcac
    10981 ggcgaagaag cccttgccgc cgcgcaacgc atttccgaaa gcctgtttgc cgaagaccaa
    11041 agtcggctta ccgaaagcga cttcgagcag ctcgccctcg acggcctgcc tgcatttgaa
    11101 gtttcagacg gcatcaacgc cgtcgaagcc ttggtcaaaa ccggcttggc agcgtccaac
    11161 aaagaagcgc gcggctttgt caacgccaaa gcggttctgc tcaacggcaa accggctgaa
    11221 gccaacaacc ccaaccacgc cgccgaacgc gccgacgatg cctatctgtt gataggcgaa
    11281 tacaaacgtt tcggcaaata caccatcctc cggcgcggca aacgcaacca cgcgcttttg
    11341 gtttggaaat aatccgattg ccgcagaaat gccgtctgaa gctttcagac ggcattttta
    11401 tcaaatgcaa aacaccctgc gcctgccgat atgtcgtcat ttccatgcag gcgggaattc
    11461 aaacttgtcc gcacggaaac ttatcgggca aaacggtttc ttcagttcta cgttctagat
    11521 gcccgctttc gtgggaatga cggttcagtt gctacggtta ctgtcaggtt tcggttatgt
    11581 tggaatttcg ggaaacttat gaattgtcat tcccgcgaaa gtgggaatcc aggactcaaa
    11641 atctcaagaa accgttttg
//
LOCUS       DAXFZR010000069        11572 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000069 DAXFZR010000000
VERSION     DAXFZR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.069"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..192
                     /locus_tag="ACFJAS_001658"
     CDS             <1..192
                     /locus_tag="ACFJAS_001658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982852.1"
                     /translation="PTAAKPQTWRLLYAVRRAFGRHFSLILLGEWLTLNVSTFIIQFI
                     GASGADTGRAPPKRGLYRE"
     gene            347..514
                     /locus_tag="ACFJAS_001659"
     CDS             347..514
                     /locus_tag="ACFJAS_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982853.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            674..1519
                     /locus_tag="ACFJAS_001660"
     CDS             674..1519
                     /locus_tag="ACFJAS_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC3982854.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGCQVGPKTTADDRTGLRRAVAALAGRKGIDYSSAYSMVHQRFNVESVEDLPAGK
                     LPEAVAYVHALTLHTGLTGEVLDAPPKAEPKLPIDGNSLADIAAMVYYGTRMIESGKD
                     VSAPLKQLGCRQAVTMWTVWHETRPILKRSAAALEVLRGYADKDASDRIAACLEGIYR
                     KAAVR"
     gene            complement(1556..1705)
                     /locus_tag="ACFJAS_001661"
     CDS             complement(1556..1705)
                     /locus_tag="ACFJAS_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982855.1"
                     /translation="MSAEEFCRKQIAYWLNESRKASDNADLKAFEFAGRELADYREMS
                     ERYAA"
     gene            complement(1708..2382)
                     /locus_tag="ACFJAS_001662"
     CDS             complement(1708..2382)
                     /locus_tag="ACFJAS_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982856.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDAPHL
                     NRHFWI"
     gene            complement(2379..2864)
                     /locus_tag="ACFJAS_001663"
     CDS             complement(2379..2864)
                     /locus_tag="ACFJAS_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982857.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIV
                     Q"
     gene            complement(2870..3343)
                     /locus_tag="ACFJAS_001664"
     CDS             complement(2870..3343)
                     /locus_tag="ACFJAS_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982858.1"
                     /translation="MAEIYVKNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADASE
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAKVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(3398..4693)
                     /locus_tag="ACFJAS_001665"
     CDS             complement(3398..4693)
                     /locus_tag="ACFJAS_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFC3982859.1"
                     /translation="MAQKTNTAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVAANGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(4714..>11572)
                     /locus_tag="ACFJAS_001666"
     CDS             complement(4714..>11572)
                     /locus_tag="ACFJAS_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFC3982860.1"
                     /translation="AGAEGGIGGNRKAGRPAPEGAQALRAAADGGAEQGGAGFDTAHH
                     DQSHPALRQFADRTKQEEAGRFFSGPADRNTPRAEELARGMEKQPDVSGIPSEDGAEF
                     SDTGVMPDGLARQYADMAAKYRAKPSEAVGINPDDGAVSAAAALAADSGAAVPSAVSD
                     DMETPPVADDAPSGRSADADRGGVPSAYGNVRSGGAPRGAASVAPGGSAAAASGGIAR
                     VSPLPAGQYFDGLDTRGRKALAKEAGLDIKGVADFGQIAWPVRRKIEQAYHARIEADY
                     QAASEAKQGYLPPPVRMADAVPVPKKGFSVPADALDKESRRRFDALPEGVRRHAQTVA
                     DYTADGIMRREAGMADMRGHYPEGLAESAGAVRAYRAQHPESADVLDRLNRAVYGYRR
                     NNGWNVPLLSREGERLQGVRTALPDDGASEAVVGGGRGLTRALPTEDKGLAQDVRQDV
                     RQGLTQGGRGLTPDAGADANAAALQGLPESAAVASGNAPARRQNLQVRARAEGAAPGL
                     SASENLAGTDGGKRAPVAGKRPDTVLPVLNPQVAESAVKVSPKKLMADAAADFTRRLA
                     ADRRRPEKAGVPLGDGEYRFAHTDRRHIDALAGVPGRPGKGGMPEEFADMAGPSNSDG
                     LVSDGRRYLKGREAETLQAGGLSEAVPSEPGRDYRPTQEARAPARVMTRPRDAAADGK
                     PAGRAQPARAKDAPVAGKAAAAKNAATEKPSSDKVRNIEAGKSHFDGGKGKSAAAQGA
                     ATEKPSSDKAAKPETFAKTASDNPEEARRKARVLQGGPVYTVKGRQAPQGFKALREHA
                     ENIKKRLAESIGGLAERVDVAAVSETAPDKAQMLLSQRVEGWFDGRTGKITLVAENLT
                     PERAVWAAWHELGHRGFAADGFAKYREELERADGNGLIRRIADAVQEGREGTGDAAAS
                     VRPAAVEEAVAELYAAQRTGGWAGIENRYGVKVGNGLKRGIAGVLARIGALLRRVLQR
                     LAGKAGGAMSDADVFAMLADLHGNVEGARDAPWDGNHRAVMFARAEDGAAERSKSESL
                     EKLRRAETIRISGREVPEGGNLREYKRNALEYGKSLRGPYVNKDTGREISLGRAGITE
                     VLHHDVGNPEHLQSIAAIPQIIEKSIYIDTLPNEDKAKNPDIREYEYYVAGLNIGGAD
                     YTVKAAIAVATTGDKYYDHKLTGIEKGDLLEMTSRLSSAEISNQSPLSGIDDKRLLQI
                     LQDKDAGKGGIADFDTEAVRFSRAANIGAAIGRITGKKSDLRNALKDRWDASKGIQLQ
                     FLGRRQIEDIYGGVLDGLKEYGRLSELFGADANKAVTEADKVVREWGRLKEEDAKALA
                     DLMHDATLAKVDADPLMRKDAQKRLDGIRTALDIADGKIAKAEAAVASAGARTARADA
                     AYNKAQRAADKAAYALEKAQEKHGREILADEADMRLRRLFYADSEAKRALRRAGADAA
                     AESRAKTDAVRMLEQARADVKRLEKDEVGAQKALEGLALLNRRFAGLPDAAQRVYRKA
                     RDDYRAHFGQVRDALAERLARAGQDAETVRRLKERFDNELGGVYFPLARFGDYLVVVK
                     DADGNSANVSRAETLSEAEKLRDALKADFGPGFKVSPVMKSRDYIRSRDAVGSGFMRE
                     LGEAVGMLDLDPAQRARLNDTLTQLYLNSLPDTSWAKHGIHRKGVPGFSDDARRAYAQ
                     NMGSGANYLAKLRYADRMAEQLDVMQDFVDGRKYEEGFDQRQLQRVADEMRKRHEAVM
                     NPNPSKLAQALTGFGFLWMMGMSPASAVVNLSQTAMVAYPVMAAKWGYAGAARELLRA
                     SKQIGLRFGEKFNTIEDSLNGDEKAAFRKAADYGVIDLSQAHDLAGVANGDPGLAGSA
                     WQKVMDKAAWLFHHAEKFNRQVTFVAAYRLAKRAGADSEAAFEQAKKATYDGHFDYAA
                     QNRPRFMMGNAAKVVFLFKQYSQNILYALGRNAYLAFKGDKEARKTLAGLLVSHAMAS
                     GILGLPFVSTLLAVASMLGSDDDDPWDAEAALRNMLADAFGDKAGEVLAKGFSRLTPL
                     DVSGRLGLDQLVFPDIQDGLEGKKWAESLVVGSTGAVVGAGIGAADGGADKVIQAKDG
                     KHTIPYEKLVEAREAEKSAKAEAQALRERIAQLEKGAEKPGVETADGSDNSLFGDFSD
                     EDVKKGVEKLIQEKLAGYEADMKRQEAAKAHYREIYTAHPDADSIVESRELEEWLDAQ
                     NPLVRKAFNDALKDGTAAEVIGAFDMFKAAKSAAEPEKPAEKPPAGKNTPNTLSDIPA
                     GRDHTASDGPPDYLSGNALAEKLASMTEEQVEKFLNS"
BASE COUNT         2442 a         4102 c         2762 g         2266 t
ORIGIN      
        1 ccgaccgccg ccaagccgca aacttggcgg cttctttatg ccgtccgaag ggctttcgga
       61 cggcattttt ctttgatttt gttgggggaa tggttgactt tgaacgtgtc cacgtttata
      121 attcaattca tcggcgcatc gggtgccgat acgggaagag ccccaccgaa gcggggcttg
      181 tacagggagt aggaaaatga agctcatcct tcaaattctc atcctggttt taatcttgat
      241 gctggcaggc gatacgccta ttagcggata aaaccggctg gggcggtagc cgccgcccca
      301 gtccctatct tacaaaaggc agttcaaaaa atcaaggagt ttgcctatgg ttaatgaaaa
      361 actggcagaa aaccgaaagc gttacgagca aaaacgagtc attaaaaaag tctcgtttaa
      421 tgcggaaaca gaaaaagagc ttttggaata tgcccaaaat ctcgattttt cccaatgggt
      481 taaatcaatc ataaaagaaa aaatcaaaaa gtaattgact ttgaacgtgt ccacgtttat
      541 tattcttaca acggcacagc cgttaatgga aaaccgaacc aagcgggaac ttggttcggt
      601 taagtgaaaa gcagttctag gaaaactctt ttcgcgtctt ttaaataacc tctaaaagaa
      661 agactattct atcatgaatg ctgtccaagt tttaaacttt caacaaaact ccgtccgtac
      721 tgttgccgat aacaaaggcg agttgtggtt tttggcaaac gatgtttgcg agattttagg
      781 ctacaccaac cctcgccgaa cggttgacct gcactgcaaa agcaggggcg taacaaaacg
      841 gtacaccccc accgccagcg gcgaacagga gatgacctat attaacgagc cgaatctcta
      901 ccgcttgatt atcaaatccc gcaagcctgc cgcagaagcg tttgaagaat gggtaatgga
      961 aaccgtcctg cccgccatcc gcaaaacagg cggctgccaa gtcggaccaa aaaccaccgc
     1021 cgacgaccgt accggattgc gccgggccgt tgccgcgctt gccgggcgca aaggcataga
     1081 ctactcctcc gcgtacagta tggtacatca acgcttcaac gtcgaatccg tcgaagacct
     1141 ccctgccggg aagctgcccg aagccgtcgc ctacgtccac gcattgacac tgcacacggg
     1201 cttgacaggc gaagtgttgg acgcaccgcc caaagccgag ccgaaactgc ccatcgacgg
     1261 caactcttta gccgacattg ccgctatggt ttattacggc acacggatga ttgaatcggg
     1321 caaagacgtt tccgcaccgc tgaagcagct cggctgcagg caggcggtta cgatgtggac
     1381 ggtttggcac gaaacccgtc caatcctgaa aagatccgcc gcggccctcg aagtgttgcg
     1441 gggatatgcg gacaaagacg cctccgaccg catagccgca tgtcttgaag gcatttacag
     1501 aaaggctgcg gtaaggtaaa cggaaaatgc cgtctgaatc ttcggacggc atttttcacg
     1561 cggcatagcg ttccgacatt tcccgataat ccgccaattc ccgtccggcg aactcaaagg
     1621 ccttcaaatc ggcgttatct gatgccttgc ggctctcgtt cagccaatag gcgatctgtt
     1681 tcctgcaaaa ctcttcagca ctcatcgtca aatccaaaaa tgcctgttca ggtgcggcgc
     1741 atcctcacgc gtgatacggc gcaaatcact gtcggccggc aggccgaaat acagctcgaa
     1801 acgcctcctt gccgattccg aacgctgcgg gtcgaaagtc tccgcatccg ggatactgaa
     1861 agcctccgcc aaagcccaat aaaccaaatt ccgatgatgt atggaattga tttccggctc
     1921 gtccccgtcc cccgccatat cccgcggcag gcaatacccc ccggcaaaca acctgccgtc
     1981 acggtccggc gcaggcgcaa gcgtcagctt cccgtcgccc tgcaccgcat aaaccggcag
     2041 ccccgtttgc gccccgtcgc gccaacccgg ggcgcaaaca tccgccgcct ccggcgaaac
     2101 caacttcagg cacaccggct tgccgtcgga cacaaaacgg atactgtcga tttcatacgc
     2161 ataacccgcc gcaaacccgc ccgccgcata agcataaagc ggccgccctt tttccacatc
     2221 cgttacaaac gcatcgtccg aatgcagcag ccggccgcgc aggcacgcct cgtgaaccgc
     2281 atcgttcaac catcccgaca cctcttcgtc gctccaaaaa tacggctcgg ccttatcatt
     2341 ggcaagcacg cggaaacgcg caatcaaagc cttcaaattc attgcacgat accgaactga
     2401 cgaaccatcc gaaccacgtc cccgcgcaaa ttctccgccg atttgcgctt atccaaaccc
     2461 tggccgtaat tcgccttcgc atacagttca agctcgtcct tgcccatccg gttgatttgc
     2521 aggatcacat cctgcaccgc gtcaaacgcc gccgtttccc gttcgagttc cgaagcgccc
     2581 gccttttcca aagcctccgt atcatccgcc ttttccacgc gtttgcccgg gacggcggca
     2641 aacacatcgc cgtggcgcaa caactttgcc gcagccccgg ccgacacctc gcgcacctgg
     2701 ccgtcttcga aaaccaagcc cgtatgatag atacggtcgc gccacacggc acgcccgccg
     2761 atatacttga taaaaacctt atccatccaa ccccccgccg ccgtccggac ggcattgtca
     2821 aatacagcaa cgccgcaaag gcaaatcccc gggcggcgtt tcattccgct tacaactggc
     2881 cgaacttctc gccgtaaatc aaaatatcga tatcggcggc cttcttattt tccgcccctt
     2941 taagcgtaac cgtcagcaaa gcctgcttgg gcagcgtaac cagtttggca gactggcagc
     3001 ggatacgccc ggcagacgca aagtccgcgc ccgaagcaaa ataagccgca tcctgaggca
     3061 ctttcgcatc atccgcacca tccgcatacg caaagccgac atcggcagtg ataccggaag
     3121 acatcgccgt cttcacaata atctgcgcgt catccaaacg gaaaccctgt tccaagacac
     3181 ccagcgccaa cacatcgccc gccttaggcg gctcagaagc atccgccccc aagatcgcac
     3241 cgtcctgctt cgcaacgata cggtaatgct ccgccgccag attgccgtaa ggcacgccgc
     3301 cgaaacggtt gccgccattg tttttcacat aaatctcagc cataaaaaac ctcaacccaa
     3361 caaataggaa aagccgccct ttcaagcggc attcgaatca gcggataccg ccgtgcaaag
     3421 gaaccaccgt atccacaacc gtgacaccgt agtcggtaat ttccttcgcg cccccgccga
     3481 catccacggc aaagcgcgtt ttcgccacac cgcgtatcgt accgacgagc gtttccacac
     3541 ggttgccgtg gtcaagctcc ttctcagacc aaaagaaagg cataccgccg tgtttgccgg
     3601 tattcgcaaa cgcctccaac accgcctggc cgcccaaaat aaccgaacgg tcgaccgcaa
     3661 acttgtccgc aaacgaagcc gggattttca agcccgattc cgcctcgctg aacttatcgg
     3721 cgcaatactt catctcatcg cccgcataga aacggatggg gcgcggcatt ttcaccagga
     3781 taaagccgtt ccacaaaccc gcatcgccca aaaacagcgg attttgattt gcctgggagg
     3841 cgcgcgcgat tgccgaagcc tgaagctggc ggaatttcgg atcggcggca aaacggttgt
     3901 actgcgccgg ggaaagcagc cacacgcgca aaggcgaatc acccgccgcc ttgtcgccct
     3961 caaacttcac aatcggcggc ggcagcggaa tctggtcgag cacggtacgc atactgtcca
     4021 ccgtatccat cgtaaacaaa tcggtagagg caatcttcaa ctcgccgccg ttcgccgcaa
     4081 cgcccgttac cgcatcgccc gaagccgtaa aatggcggtt tttggacggg gctttgaccg
     4141 gattgaccgc ataatcattg aatttcgggt cgcccgccaa aggcacgccc cattcgatat
     4201 tgtcatgaaa accgcgcgcg cccgccatat gcaccaacag cgtctggtcg gcataacggt
     4261 ccatcaggct ttgcgccgcc ggacgaatca gcgcgcgata atcggcaggg ctgcgctgat
     4321 tcgtcatcgt gccgccgccg tcaacgggga aacgcgcctg attcacacgc aaacccgcct
     4381 ccgacaggct catccccacg cccctgcctt ccgccgtgtt gtcgcccata atcggcaggg
     4441 cggaaacagg gttgaccaaa ttgaaacgga tttcgtcacc catgccgcgc gtcaaatcct
     4501 gacagcgcac gaccggcata tgctgggtcg tctgtttgcg caaagtcgcc tccgcacccg
     4561 cggtaccggc aggcatcttg cccgccaaac ggttcagcgt actgttgcgc tgcatatgca
     4621 tcgcaaacag ccccgccgcc tgcttcatca tcgcctgcgg gtcgccgtag gccgtattcg
     4681 tcttttgtgc cataaagagc ctccctcaaa aactcaagaa tttaaaaact tctcaacctg
     4741 ctcttccgtc atggaagcca gtttttccgc cagcgcgttg ccgctcaaat aatccggcgg
     4801 gccgtccgaa gccgtatggt cgcgccccgc cggaatatcc gacagcgtat tgggcgtatt
     4861 cttcccggca ggcggctttt cggcgggttt ttccggttcg gcggcggatt ttgccgcctt
     4921 aaacatatcg aacgccccga tgacctcggc ggcagtcccg tccttaagcg cgtcattaaa
     4981 cgccttgcgg acaagcgggt tctgcgcatc cagccactcc tccagctcgc ggctttccac
     5041 aatcgaatcc gcatcggggt gcgccgtata gatttcgcgg taatgcgcct ttgccgcctc
     5101 ctgccgcttc atgtccgcct cataacccgc cagcttctcc tgaatcagct tttccacgcc
     5161 ctttttcaca tcctcgtcgg aaaaatcccc gaacaacgaa ttatcggaac cgtcagccgt
     5221 ttccactccg ggtttttccg cccccttctc aagctgggca atgcgctccc tcaaggcttg
     5281 ggcttcggct ttcgccgact tttccgcctc gcgcgcctct accagctttt cataagggat
     5341 agtgtgtttg ccgtccttgg cctgaataac cttgtccgca ccgccgtccg ccgcgccgat
     5401 acccgcgccg accaccgcgc ccgtactgcc gaccaccagc gattccgccc acttcttacc
     5461 ctccaaaccg tcttggatat cggggaaaac caactggtcc aaacccaaac gccccgacac
     5521 gtccagcggc gtcaggcggc tgaagccctt ggccaacacc tcgcccgcct tatccccgaa
     5581 agcatccgcc aacatattgc gcaacgccgc ttccgcatcc cacgggtcat cgtcgtcact
     5641 gcccaacatc gaagccaccg caagcagcgt cgacacaaac ggcagcccca agatgcccga
     5701 agccatcgca tgcgagacca acagccccgc cagcgtctta cgcgcctcct tatccccctt
     5761 aaacgcaagg tacgcattgc gccccagcgc atacaggata ttctgcgaat actgcttgaa
     5821 caggaagacc accttcgccg cattgcccat cataaagcgc ggacggtttt gcgccgcata
     5881 gtcaaaatgc ccgtcatacg tcgccttttt cgcctgttcg aaagccgcct cgctgtccgc
     5941 ccccgcccgt ttcgccaaac ggtaggccgc gacaaacgta acctggcggt taaacttctc
     6001 cgcatgatgg aacagccagg ccgccttatc catcaccttc tgccaagccg accccgccaa
     6061 ccccgggtcg ccgttggcca cccccgccaa atcatgcgcc tgcgacaaat cgatcacacc
     6121 gtaatccgcc gcctttcgga acgccgcctt ctcatccccg ttcaaactgt cctcaatcgt
     6181 attgaacttc tccccgaacc tcagcccgat ctgttttgaa gcccgcagca attcccgcgc
     6241 cgcaccggca taaccccact tcgccgccat caccggataa gccaccattg ccgtctgcga
     6301 caggttcaca accgcagaag cgggcgacat ccccatcatc cacaaaaagc cgaagcccgt
     6361 caaagcctgc gccagcttgg aaggattcgg attcatcacc gcctcgtggc gtttcctcat
     6421 ctcatccgcc acacgctgca actgacgctg gtcgaaaccc tcctcatatt tgcgcccgtc
     6481 cacaaaatcc tgcatcacat ccaactgttc cgccatacgg tccgcatagc gcaacttcgc
     6541 cagataattc gcaccgctgc ccatattctg cgcatatgcg cgcctcgcat catcgctgaa
     6601 gcccggcacg cccttgcggt ggatgccgtg tttcgcccaa gacgtatcgg gcagcgagtt
     6661 caaataaagc tgcgtcagcg tatcgttcaa tcgggcccgt tgcgccggat ccaaatccaa
     6721 cataccgaca gcctcgccca gctccctcat aaaaccgctg ccgaccgcat cgcggcttcg
     6781 gatataatcc cgggacttca tcacgggcga aaccttaaac cccggcccga aatcagcctt
     6841 cagcgcatcg cgcagcttct ccgcctcgct caaagtttcc gcgcgggaca cattcgcact
     6901 attcccgtcc gcatccttga ccaccaccag ataatcgccg aaacgcgcca aggggaaata
     6961 cacaccgccc agctcgttgt caaaacgctc cttcaggcgg cgcaccgttt ccgcatcctg
     7021 ccccgcacgc gccaaccgtt cggcaagcgc atcgcgcacc tgcccgaaat gcgccctata
     7081 atcatcccgc gccttgcggt acaccctctg cgccgcatca ggcagcccgg caaaacggcg
     7141 gttcagcaaa gcaagcccct ccaaagcctt ttgcgcccca acctcatcct tttccaaacg
     7201 cttcacatcc gcgcgcgcct gctccaacat ccgtaccgca tccgttttag cccggctttc
     7261 cgccgccgca tccgccccgg cgcgcctcaa cgcccgcttc gcctccgaat ccgcataaaa
     7321 cagacggcgc aggcgcatat ccgcctcatc cgccaaaatt tcccgaccgt gtttttcctg
     7381 cgccttctcc agcgcataag ccgccttatc cgccgcccgt tgcgccttat tgtaagcagc
     7441 atccgcacgc gcggtacgcg caccggcgga agcaacggcc gcctccgcct tcgcgatttt
     7501 accgtccgca atatccaaag ccgtccggat gccgtccaaa cgcttctgag catcctttcg
     7561 catcagcggg tcggcatcca ccttcgccag cgtcgcatcg tgcatcagat ccgcaagcgc
     7621 tttcgcatcc tcctccttca acctgcccca ttccctgaca accttgtccg cctccgtaac
     7681 cgccttgttc gcatccgcgc cgaagagttc cgacaaacgc ccgtattcct tcaggccgtc
     7741 caaaacgccg ccgtaaatgt cctcgatctg ccgcctgccc aaaaactgga gctgaatccc
     7801 cttggaagca tcccagcggt ctttcagcgc gtttctcaaa tcggattttt tacccgttat
     7861 acggccgatt gcggccccga tgttcgccgc acgggaaaaa cgcaccgcct ccgtgtcaaa
     7921 atcggcaatg ccgcccttgc ccgcatcttt gtcttgcaaa atctgcaaca ggcgtttatc
     7981 atcaatgccc gataaaggcg attgattgga aatctccgca ctggataagc gagaggtcat
     8041 ttccaaaagg tcgccttttt ctattcccgt caatttatgg tcatagtatt tgtcgcctgt
     8101 tgtcgcaacg gcgattgccg cttttaccgt gtaatcagcc ccgccgatat tcaagcctgc
     8161 aacataatat tcatactctc gaatatcggg atttttggct ttgtcttcat tcggcaaagt
     8221 atcgatataa atcgacttct cgataatctg cgggatggcg gctatgcttt gcaaatgttc
     8281 agggttgccg acatcgtgat gcaacacctc cgttatgccc gcccgtccga gtgaaatttc
     8341 acgccccgtg tccttattca cataaggccc gcgcaaagat ttgccgtatt ccagcgcatt
     8401 gcgcttatat tcgcgcaaat tgccgccttc cggaacctcc ctgcccgaga tacggatggt
     8461 ttccgcacgg cgcagcttct caaggctttc cgacttggaa cgttccgccg caccgtcttc
     8521 agcccgcgcg aacatcactg cacgatgatt gccgtcccaa ggcgcatccc gcgccccttc
     8581 cacattcccg tgcaaatccg ccagcatcgc aaacacatcc gcgtccgaca tcgcaccgcc
     8641 ggccttgccc gccaggcgtt gcagcacacg gcgcaacagg gcgccgatac gcgccaacac
     8701 gcccgcaatg ccgcgtttca aaccgttgcc gaccttcacg ccgtaacggt tttcaatgcc
     8761 cgcccaaccg ccggtacgct gcgccgcata aagctccgcg accgcctctt ccaccgcggc
     8821 ggggcgcacc gaggccgccg catcgcccgt gccttcgcgc ccttcctgca ccgcgtccgc
     8881 aatgcgccga atcaggccgt tgccgtccgc acgttccaat tcttcacggt acttggcgaa
     8941 accatccgcc gcaaagcccc tgtgccccag ttcgtgccac gccgcccata ccgcacgttc
     9001 gggcgtaagg ttttccgcca ccagcgtgat tttgcccgtc ctgccgtcaa accagccctc
     9061 cacacgctgc gacaacagca tctgcgcctt gtccggcgcc gtttcggaca cggcggcgac
     9121 atccacccgt tccgccagtc cgcctatgct ttcggcaagg cgttttttga tattttcggc
     9181 gtgctcgcgc aatgccttaa aaccttgcgg cgcctgacgc cctttcacgg tataaacagg
     9241 ccctccctgc aacacacgcg ccttgcgccg tgcctcttcc ggattgtccg aagccgtttt
     9301 cgcaaacgtt tcaggcttgg cagccttatc cgaagacggc ttttcggttg ccgcgccttg
     9361 tgcggcggcc gacttgccct ttccgccatc gaagtgggat tttcccgctt cgatatttcg
     9421 caccttatcc gaagacggct tttcggttgc cgcatttttt gcagcagccg ccttgcccgc
     9481 aacaggcgcg tcttttgccc gggcaggctg cgccctgccc gccggtttgc cgtcggcggc
     9541 ggcatcgcgc ggccgcgtca tcaccctggc cggcgccctt gcttcctgcg tcggacgata
     9601 atcccgaccc ggctcggacg gcacggcttc cgacaaaccg cccgcctgca aggtttccgc
     9661 ctcccgcccc ttcaaataac ggcggccgtc ggagacaagg ccgtcagagt tggaaggacc
     9721 ggccatatcg gcaaactcct ccggcatccc gcctttgccc ggcctgcccg gcacgcccgc
     9781 aagcgcatca atatgcctgc ggtccgtatg cgcgaaacgg tattcgccgt cccccaaagg
     9841 cacacccgcc ttttccggcc tgcgcctgtc cgccgccaaa cggcgcgtga aatccgccgc
     9901 cgcatccgcc atcaatttct taggcgatac cttgactgcc gattccgcaa cctgcggatt
     9961 caataccggc aacaccgtat cggggcgttt gcccgcaaca ggcgcacgtt tcccgccgtc
    10021 cgtcccggca aggttttcag acgcggacag gccgggcgcg gcaccttccg cgcgcgcccg
    10081 aacctgtaag ttttgccgac gggccggcgc attgccggac gcaacggcgg cggactcggg
    10141 caaaccctgc aaggcagccg catttgcatc cgcccccgca tcaggggtca aacccctgcc
    10201 gccttgggtc aaaccctgcc gcacatcctg ccgcacatcc tgcgccaaac ccttatcttc
    10261 cgtgggtaaa gcccgggtca aacccctgcc gccgccgaca acggcctcag acgcgccgtc
    10321 atccggcaac gccgtccgaa ccccctgcaa acgctccccc tcgcggctca acagcggcac
    10381 gttccaaccg ttgttgcggc ggtaaccgta aaccgcacgg ttaagcctgt ccaacacatc
    10441 cgccgattcc ggatgttgcg cacggtaagc gcgtacagcc ccggccgatt ccgccaaacc
    10501 ctcaggataa tgtccgcgca tatccgccat acccgcctcc cggcgcataa tcccgtccgc
    10561 cgtatagtcc gccaccgtct gcgcatgacg gcgcacccct tccggcagcg cgtcaaaccg
    10621 cctgcgcgat tccttatcca acgcatcggc agggacggaa aacccttttt taggaacagg
    10681 cacggcatcc gccatacgca cgggcggcgg caggtaaccc tgtttggctt cggaagccgc
    10741 ctgataatcc gcctcaatcc gcgcgtgata cgcctgctcg atttttcggc gcacaggcca
    10801 ggcgatttgc ccgaaatccg caacgccctt aatatcaagg cccgcctcct tcgccaaagc
    10861 cttgcgcccg cgcgtatcca agccgtcgaa gtattggccc gcaggcagcg gcgagacccg
    10921 cgcaatcccg cccgaagcgg cggcggcaga accgcccggc gcaaccgaag ccgcaccgcg
    10981 cggcgcaccg ccggaacgca cattgccgta agcggacgga acaccgcccc tgtccgcatc
    11041 cgccgaccgt ccggacggcg catcatccgc aaccggcggc gtttccatat cgtcagacac
    11101 cgcagacggc acagccgcgc ccgaatccgc cgccaaagca gcggccgcag aaaccgcacc
    11161 gtcatccgga ttaatcccca ccgcctccga cggcttggcc cggtatttag ccgccatgtc
    11221 cgcatactga cgcgccaaac cgtccggcat cacgcccgta tccgaaaatt ccgccccgtc
    11281 ctccgacggg atacccgaaa catccggctg tttttccatc ccgcgcgcca attcctccgc
    11341 gcggggcgta ttcctgtcgg cggggccgct gaaaaaccgc cccgcctcct cctgcttcgt
    11401 acggtccgca aactgccgca aagccggatg cgactgatcg tgatgcgccg tatcaaaacc
    11461 cgcgccgccc tgttccgcgc cgccgtccgc cgccgcacgc aacgcctgcg ccccttccgg
    11521 ggccggcctg cccgccttcc tgttgccccc gatacccccc tccgcacccg cc
//
LOCUS       DAXFZR010000070        11559 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000070 DAXFZR010000000
VERSION     DAXFZR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.070"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            164..880
                     /locus_tag="ACFJAS_001667"
     CDS             164..880
                     /locus_tag="ACFJAS_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC3982861.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            1029..1931
                     /locus_tag="ACFJAS_001668"
     CDS             1029..1931
                     /locus_tag="ACFJAS_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC3982862.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1974..2748
                     /locus_tag="ACFJAS_001669"
                     /pseudo
     CDS             1974..2748
                     /locus_tag="ACFJAS_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            2860..4548
                     /locus_tag="ACFJAS_001670"
     CDS             2860..4548
                     /locus_tag="ACFJAS_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC3982863.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPGAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDTPVFNRTVTLKDSWQPK"
     gene            4640..5596
                     /gene="gshB"
                     /locus_tag="ACFJAS_001671"
     CDS             4640..5596
                     /gene="gshB"
                     /locus_tag="ACFJAS_001671"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC3982864.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKAFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            5755..6138
                     /locus_tag="ACFJAS_001672"
     CDS             5755..6138
                     /locus_tag="ACFJAS_001672"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC3982865.1"
                     /translation="MEPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            6270..6743
                     /locus_tag="ACFJAS_001673"
     CDS             6270..6743
                     /locus_tag="ACFJAS_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC3982866.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            6868..7971
                     /gene="mnmA"
                     /locus_tag="ACFJAS_001674"
     CDS             6868..7971
                     /gene="mnmA"
                     /locus_tag="ACFJAS_001674"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC3982867.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVEQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            8042..9712
                     /locus_tag="ACFJAS_001675"
     CDS             8042..9712
                     /locus_tag="ACFJAS_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC3982868.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPNEKTGEALKVFVVKKDPS
                     LTKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            9824..11458
                     /locus_tag="ACFJAS_001676"
     CDS             9824..11458
                     /locus_tag="ACFJAS_001676"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC3982869.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         2821 a         3148 c         3093 g         2497 t
ORIGIN      
        1 attacctcgc cttgccgtac tatttttgta ctgtctgcgg cttcgtcgcc ttgtactgat
       61 ttaaatttaa tccactatac tttaatttct cctacacgat ttgaaacaca gccgtcttaa
      121 aacatcttaa aaaatttgaa agcgtaaaaa tacggaaaac atcatgaact ttgaaaacga
      181 cgacattatc catgcgccga ccacgtcttc cctgattctc gaagagcggc acgattcgga
      241 gctgttccgt gtttacgccc tgattttgga cggcattacc gatcaggtgc tgctgcccgg
      301 caaaaagctg accgaatccg aactttgccg tcagatggtg tgttcgcgca acaccgtccg
      361 cggcgcgctg tcgcttttgg cgcacgacaa gattgtcgat ttgcaaccca acaggggcgc
      421 gttcgtccac gttcccgatt tgaaagaaat gcaggatgtg ttcaatgcgc gcatcgaaat
      481 ggagacgatg attttgaaca tcctcgccgg cctgccggat ttggaaacgc gcctcaagcc
      541 gctttatgcg atgataaggt gcgaagaaga ggcctccggc aggggcgacc gcgtcggctg
      601 gaaccgcctg tccaatgcct tccacgtcga actggcgcgc ctggtgggca acgatgtgct
      661 gttcgacatt atgaacacgc tgtgcgcgcg gtcttccctg attgtcgccg tggcgggcgt
      721 gcatcgcgag aaaaaacacg ccatcaatac gcatacgcat tccgaacacc gcgaaatcct
      781 cgacctgctg ctggcgggca ggcgcaaccg cgtggtcaaa atcctgcgcc gccatttggg
      841 caactgtatg gagcgtttgg aaaagacttt ggaagattga atgccggagc ggataagccg
      901 cctgaacctt caggcggttt tttaatggcg aacctgatgc cgtctgaaat atggatgcgg
      961 gtatctgcaa ttttcagacg gcaattttta agccgcacat atcatgcggc aataaaggag
     1021 ggtaggggat gagcagcctg atgacgttgt tttcggtatt ggtaccgatg tttgccggat
     1081 tttttatccg tgttcccaag ccttacctgc ccgcttcgga caaggtgctg tcggttttgg
     1141 tgtatgccgt gctgctgctg atcggcgtat cgttgtcgcg cgtggaggat ttgggttcgc
     1201 ggttgggcga tatggcgttg acggttctgt ggctgtttgt ttgtacggta ggggcgaacc
     1261 tgcttgcctt ggcagtgttg ggaaagttgt ccccgtggcg gataggggga aaagggaagg
     1321 gcgtttcggt cggcgtgtcg ggcagtgtga ggcagctcgg atgcgtactg atcggttttg
     1381 tgtccggcaa attgatgtgc gatatttgga tgccgtctga aaacgcgggt atgtactgcc
     1441 tgatgctgct ggtgttcctc atcggcgtac agctcaaaag tagcggcgta tcgttgcggc
     1501 aggttttgct taaccggcgg ggcatccggc tgtcggtttg gtttatattg tcatctcttt
     1561 caggcgggct gctgtttgcc gcatcggcag atggtgtgtc gtggacgaaa ggtttggcga
     1621 tggcttccgg cttcggttgg tattccctct cgggtttggt aatgaccgag gcttacgggg
     1681 cggtatgggg cagcatcatg ctgctgaacg atttggcacg agagctgttt gcactggcat
     1741 ttattccgct gctgatgaag cgttttccgg atgcggcggt gggggtcggc ggcgcgacca
     1801 gtatggattt cacattgccc gtaattcagg gtgcgggcgg tttggaagtc gtgccggtag
     1861 cggtcagctt cggcgtggtg gtcaatatcg ccgccccgtt tctgatggtg gtgttttcca
     1921 cgctgggctg aacgcggtaa aatcggcatc ccgatgcaag gaagcagaaa acgatgaaac
     1981 cgaaaatcca aaggcatgga gagattttaa gccttgtccg ccggcatcag tttatgtcgg
     2041 tggacgagct tgccgccgca ttggacgtta ccccgcagac gatacgttgc gacatccgcg
     2101 agttggagga gggcggcagc ctgaaacgcc atcacggcgg cgcatcttcg ggcggaaact
     2161 tgccggaggg tctgcccgcc gaccgccaaa cccggtgtca aaacgaaaaa aacgccattg
     2221 cccggctgat tgcggaacac atccccgacg gttcgtcgct gttcgtcagt atcggtacga
     2281 ccatggaagc cgcggcatca gagctggtga agcggcgcag cagcctgcgg attattacca
     2341 acaatatcca cgtcgcctcc gtcgtttcgg cacgtacgga ttacacggtc atcatcacat
     2401 ccggcgtcgt ccgccctttg gacggcggta ttaccggcgt ggcgaccgtc gactttatca
     2461 accagttcaa agtcgattat gccgtgatga gcacgcacgg cgtggaaagc gacggttcgc
     2521 ttttggatta cgattacaag gaagtcagcg tcatgcaggc gatgattgcc aacgcgcgcg
     2581 tccgttttct cggcgtggat cacagcaaat tccgcagcaa cgcgctggtc aggctcggcg
     2641 gcattaccgg cgtttgacaa agtatttacc gacaggttgc ctgataccgc gatgcagaag
     2701 atgctgaaag aggcgggggt ggaatgcctg attgccgatg ccgtctgaac gctatgtcaa
     2761 agcgcgcaag tcgggtacaa taaacacatc atcaaaccgc ttcagacggc atacggaacc
     2821 tccccaatgc cgtctgaagc catctgttta aagaaaacca tgctcaataa agaccaattc
     2881 gcggacaacc atttcatccg caccatcatc gaagacgacc tcaaaagcgg caaacacgaa
     2941 gccgtccaaa cccgttttcc gcccgaaccc aacggctatt tacatatcgg acacgccaaa
     3001 tccatctgcc tgaacttcgg tttggcgtat atttacgacg gtttgtgcaa cctgcgtttc
     3061 gacgacacca acccggaaaa agaaaacgac gaatacgtca acgccatcaa agaagacgtc
     3121 gagtggctcg gtttccattg ggcgggcgaa ccgcgtttcg cttccgacta tttcgaccgg
     3181 ctttatgact acgccgtcgg tttaatcaaa gacggcaaag cgtatgtcga tgatttgacg
     3241 cccgaagaaa tgcgagaata ccgcggtacg ctgaccgaag cgggtaaaaa cagcccttac
     3301 cgcgaccgca gtatcgaaga aaacctcgac ctgttcacgc gtatgaaaaa cggcgagttc
     3361 cccgacggca gcaaaaccct gcgcctgaaa atcgacatgg cggcaggcaa catcaatatg
     3421 cgcgatcccg tcatctaccg catccgccgc gcccatcacc acaacaccgg cgacaaatgg
     3481 tgcatctacc cgatgtacga ttacacgcat tgcatttccg atgccatcga aggcatcacg
     3541 cattccttgt gcacgctcga atttgaagcg caccgtccgc tttacgactg ggtgttggac
     3601 aacatccccg cgccgcacgc cacccgtccg cgccaatacg agttttcccg tttggagctt
     3661 ttgtacacca ttacctccaa acggaaattg aatcagttgg ttgtggaaaa acacgtttcc
     3721 ggctgggatg atccgcgtat gccgaccatt tccggtatgc gccgccgcgg ctacacgccc
     3781 gaaggggtgc gcctgtttgc caaacgcgcc ggtatttcca aatctgaaaa catcgtcgac
     3841 atgagcgtgt tggagggtgc gattcgcgaa gagctggaaa actccgcccc gcgcctgatg
     3901 gcggttttga acccgctcaa agtgaccctg accaactttc aagccggcaa aacccaaagc
     3961 cgccgtgccg cgttccatcc gaaccacgaa gaaatgggcg atcgcgaagt acctgtttca
     4021 caaaccatct acatcgaagc cgacgacttt gccgaaaatc cgcccaaagg cttcaagcgt
     4081 ctgattcccg gcggcgaagt acgcttgcgc cacggctatg tcatcaagtg cggtgaagtc
     4141 gtcaaagacg aagcaggcaa tgtggttgaa ctcaaatgca gcatcgacca cgacaccttg
     4201 ggcaaaaatc cagaaggccg caaagttaaa ggcgtgattc actgggtttc cgccgaacac
     4261 gccgccgaaa tcaaagtccg tctgtacgac cgcctcttta ccgtcgagcg tcccggtgcc
     4321 gtgcgcggcg aagacggcga atacctgccg tttaccgatt tcctcaatcc ggaatccgtt
     4381 aaggaaatca ctgcttacgc cgaacctgcc gcgaaagatt tgccggcgga aagccgttgg
     4441 cagttcgagc gcatcggcta ttttgtgacc gaccgccaag accacggcaa agacacgccg
     4501 gtgtttaacc gcacggtgac gttgaaagat tcttggcagc ctaagtaaac cccatccttg
     4561 ccgtctgaat attgttcggg cggcatttct cctttacccg cgaaatgcgg cacattcggc
     4621 acaccggaaa ggaaatatga tgaaagtcct ctttatcgcc gacccgatgg caagtttcaa
     4681 aacctacaaa gacaccacct acgcgatgat gcgggaaatg gcaaaacgcg gctggcggct
     4741 gtttcatacc ttgagcgggg aattgtctgt aaacggcggt ttggtaacgg cacaggcatc
     4801 ggcatttgaa ttttcgggtg caaaaaacga tgatgaccat gaatggttta aagcggcgga
     4861 caaagttcag acggcattaa aagcatttga tgccgtgatt atgcgtaccg atccgccgtt
     4921 cgatatgcaa tacctttact ccacccaatt actgacgctg gcggaacagc agggcgcgaa
     4981 agtgttcaac agcggacggg cgatgcgcga ctttaacgaa aaactggcga ttttgaattt
     5041 cagccgcttt accgcgccca cgctggtaac gacccgttcc gccgatgtcc gcgcattttt
     5101 gaaagaacac ggcgacatca tcgtcaaacc gctcgacggc atgggcggca tgggcatctt
     5161 ccgcctgacc gaaaaagacc ccaacatcgg cagcatcctc gaaaccctga tgcggtttga
     5221 ttcccgcacc attatggcgc aacgctacat tcccgaaatc gtacacggtg acaaacgcat
     5281 cttgattatc ggcggcgaag tcgtccccta tgctttggcg cgtatcccgc aaaacggcga
     5341 aacacgcggc aatctggcgg caggcgggcg cggtgtggcg caggaattgg acggacgcga
     5401 ccgggaaatt gcagagactc tggctcccga gcttaaacgg agcggcatcc tgctggccgg
     5461 tttggacgtt atcggcagca acctgaccga agtcaacgta accagcccga ccggattcca
     5521 agaaattatg aaacaaaaaa gtttcgacgt ggcggcaatg tttgccgatg ccgttgccgc
     5581 gtggtcggta cgttaaaccg atgccgtctg aaaggctttt gcttcgtaac cgcttggttt
     5641 gttcgggcag gcagggtttt ccctcagccg gcagggatgc gcttaatgcc gtacggacac
     5701 ccctccgccc cgttttcaga cggcatatat tgaggacatt ttgaaaggat accgatggaa
     5761 ccttcctcct acgcggcaga aaaaaaaggc aaaagcggca tcaggcgcgt catcaacgca
     5821 ttcggctatt cgatagacgg catcgccgcc gcctaccgtt acgaagcggc attccgtcag
     5881 gttttgtggc tgaacgcgct gctggtgtgc gcggcatttt tttgggtttc cgaaaccgcc
     5941 gtccgcctgc cgttgattat cgcgtctttt gtgtcggtca ttgtcgaact gttcaacacc
     6001 gccgtcgaag ccgccgtcga tcatacttcg accgaaaaac acgagctggc caaacgcgcc
     6061 aaagatgcag gttctgccgc acaactggtt gccatgctga tgttggcggc ggtttggctg
     6121 tccgccctgt tcgggtaaaa cgcttgcagc aggatttgta atcctttagg attagtattt
     6181 attattcact taatctacat caaatttccg agcagtattt gtatgtaaga ttaagcacat
     6241 tccccgtctg atattaaagg agcaggaaga tgaaaaaatt attggcagcc gtgatgatgg
     6301 caggtttggc aggcgcggtt tccgccgccg gagtccatgt cgaggacggc tgggcgcgca
     6361 ccactgtcga aggtatgaaa atgggcggcg cgttcatgaa aatccacaac gacgaagcca
     6421 aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa gtgcataccc
     6481 acatcaacga caacggcgtg atgcgtatgc gcgaagtcaa aggcggcgtg cctttggagg
     6541 cgaaatccgt taccgaactc aaacccggca gctatcacgt gatgtttatg ggtttgaaaa
     6601 aacaactgaa agagggcgac aagattcccg ttaccctgaa atttaaaaac gccaaagcgc
     6661 aaaccgtcca actggaagtc aaaaccgcgc cgatgccggc aatgaaccac ggtcatcacc
     6721 acggcgaagc gcatcagcac taatctgctg gaaatatttg aaatgccgtc tgaaaaagcc
     6781 cgggctttca gacggcattt ttatgcccgc ctttaaaatg tgttaaaatc cgcctttaaa
     6841 aaccgccgtt tccaagccat cctgcgtatg aatacgacat caaacacttc caatatcatc
     6901 gtcgggcttt ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa gcagcagggt
     6961 tatcaagtgc gcggtgtgtt catgcagaac tgggaaaacg acgacaacga cgaatattgc
     7021 agcatcaaac aggattcgtt cgatgccatc gccgttgccg atattgtcgg catcgacatc
     7081 gacatcgtta atttcgccgc gcaatataaa gacaaagttt ttgcttattt tcttcaggaa
     7141 tacagtgcgg ggcgcacgcc gaatccggat gtgttgtgca atgccgaaat caaattcaaa
     7201 tgctttttgg actacgccgt agagcagggc gcggatacca ttgccaccgg acactatgcg
     7261 cgcaaagaag tccgcaacgg cgtgcattac ctgctcaaag gtttggatcg aaacaaagac
     7321 caaagctatt ttctctaccg cctcaagcct ttccaactcg aacgcgcgat ttttccgttg
     7381 ggcggtttgg aaaaacccga agtgcgccgc cttgccgccg aatttaattt gccgactgcc
     7441 gctaaaaaag acagtaccgg catctgtttc atcggcgagc gtccgttccg cgagtttctg
     7501 cagaaatact tgccgaccga caacggcaaa atggtgacgc ccgaagggaa aaccatcggc
     7561 gaacacgtcg ggctgatgtt ttacacattg ggtcagcgca aaggattggg catcggcggc
     7621 gcgggcgaac cgtggtttgt tgcggctaaa gatttgacga aaaacgaact catcgtcgta
     7681 caaggacacg accatccgct gctctatacc cgcagccttg tgatgaacga tttgagtttc
     7741 acgctgcccg aacgtccgaa ggcaggacgc tatacctgca aaacgcgtta ccgtatggcg
     7801 gacgcgcctt gcgaattgtg ctatttggat gatgaaaccg ccgagctggt gtttgacgaa
     7861 ccgcaatggg cggttacgcc gggtcagtcc gccgtgctgt acgacgtcga catctgtttg
     7921 ggcggcggca tcatccaaac gaccgacaaa cccgtcatca tcacgcgata aaggtaatgc
     7981 cgtctgaaac ggttttcaga cggcattgtt ccgctcaatt ccactttaaa gaccgatacc
     8041 tatggaaaaa atctggttag aaagctacga gaagggcgtc agtgccgaaa tcgacatcac
     8101 gcaatacaat tccgtcagcg acgtattccg ccaaagcgtg gaaaaatttg cccgtctgcc
     8161 cgcttttcaa aatatgggca aaacgctcac ttatgccgaa accggcaaac tggcgaccga
     8221 tttcgcctct tatctgcaaa acgtcctcaa gctgccgcgc ggcgaacgtg ttgccattat
     8281 gatgccgaac gtattgcaat atccgattgc ccttttcggt attttgcagg caggtttggt
     8341 ggcggtgaac accaatccgc tctatacgcc gcgcgagttg gagcatcagc tgaaagacag
     8401 cggtgcgacc gccatcatcg ttttggaaaa tttcgccaac acgctggagc tggtgctgcc
     8461 gcgcacgcag atcaaacacg tcatcgtcgc ctccgtcggc gaaatgttcg ggctgcttaa
     8521 aggttcgctg atcaatttca tcatccgaaa aatcaagaaa atggttcccg aataccgtat
     8581 tcgggaaacc gtttcctttc agacggcatt gaaagagggg gcgaagcacg ttttccaacc
     8641 tgtcgcatta aaccgcgaag ataccgcatt gttgcaatac acgggcggca cgacaggcgt
     8701 tgccagaggc gcggtgctga gccacggcaa catctgcgcc aatatgcttc aggcaaaaga
     8761 atggattaaa aaccaattgc gcgagggaaa agaaaccgtt atcgccgccc tgccgctgta
     8821 ccacatcttc gccctgacag tgaatctgat gatttttgcc aatgccggct cgaaaattgt
     8881 cctgattgcc aacccgcgcg atatgaaagg ctttatcggc gaactgaaaa agcagcgggt
     8941 taacgtattt atcggcgtga acacgctgtt taacgcgatg gtcaaccggc ccgatttcgc
     9001 cgaagtcgat ttttcaggat tgcggctgac tttgggcggc ggtatggcga cccaaaaagc
     9061 cgttgccgaa aaatggaaaa aaatcaccgg cacgcccatc gtcgaagcct acggtttgac
     9121 cgaagccagc cccggcgtgt gctgcaaccc cttaaacatc gaatcataca gcggcagcat
     9181 cggtttgccc gtcccgtcca ccgaagtcga actgcgcgac gcaaacggca aagaagtccc
     9241 cgttgggcag ccgggcgaat tgtgggtaaa aggcccgcaa gtgatgcaag gctactggaa
     9301 ccgccccgaa gaaaccgcca aagccataga cgcgtgcggc tttttggaaa ccggcgatat
     9361 tgccgtgatg gacgaaaaag gccgtctgaa gctggtcgat cgcaaaaaag acctcgtcgt
     9421 cgtttccgga ttcaatgttt atccgaacga aatcgaggaa ttcatcgcgc accacgaaaa
     9481 agttatggaa gttgcgtgta tcggcgttcc gaacgaaaaa accggcgagg cactcaaagt
     9541 gttcgtcgtc aaaaaagacc cgtctttgac caaagaagaa ctcaccgctt tctgccgtac
     9601 cggtctgacc gcatacaaag tgccgaaaga catcgaattc cgcgacgagt tgcccaagtc
     9661 caatgtcggc aaaatcctgc gccgcgagtt gcgccaaagt gccgggaaat aaagaaaaga
     9721 taccgtctga aaacagccgt ccaccgttca gacggcatcc gtccgtttgc cagaaccgcg
     9781 cgcttcacgt taaaatcacg cattccaaca tgggtattcc atcatgacca aattcatttt
     9841 cgtaaccggc ggcgttgtct cctcactggg taaaggtatc gccgccgctt ctattgccgc
     9901 catcctcgaa tcgcgcggct tgaacgttac catgctcaag cttgatcctt atatcaacgt
     9961 cgatcccggc acgatgagcc cgttccaaca cggcgaagtg ttcgtaaccg acgacggcgc
    10021 ggaaaccgac ctcgacttgg gacactacga acgtttcatc gattccacga tgacccgccg
    10081 caacagcttc agcacgggtc aggtgtacga aaacgtcatc gccaaagaac gacggggcga
    10141 ctatttgggc ggcacggttc aagtcatccc gcacattacc gacgaaatca aacgccgcat
    10201 ccacgaaggc gcggcgggtt acgatgtggc gattgtcgaa atcggcggta cggtcggcga
    10261 catcgaatcg ctgccgtttt tggaagccat ccgccagatg cgaagccagt tgggacgcaa
    10321 caacaccctg ttcgcccact tgagctacgt tccctacatc gccgccgcag gcgaaatcaa
    10381 aaccaagccg acccagcaca ccgtgaaaga aatgttgagc atcggcttgc aacccgacat
    10441 cctgatttgc cgtatggaca ggaaaatgcc ggcagacgaa cgccgcaaaa tcgccttgtt
    10501 ctgcaacgtg gaagagcgcg cgattgtcgg cagctacgat gtggacagca tctacgaatg
    10561 ccccgaaatg ctgcacgacc aaggcatcga caacatcatt accgagcagt tgcagcttaa
    10621 cgtgcagcag gcggatttga ccgcgtggaa aaaaatcgtc cacgccgtca aaaacccgaa
    10681 acacaccgtc aaaatcgcga tggtcggcaa atacgttgat ttgaccgaat cctacaaatc
    10741 attgattgaa gccttgaaac acgcgggcat ccataccgaa accgatgtgc agattacctt
    10801 cgttgacagc gaaagcatcg agaaaaacaa gggcgacgtt tccgtactca aagatatgga
    10861 tgccatcctc gttcccggcg gcttcggttc gcgcggcgtg gaaggcaaaa tcgccgccgt
    10921 gcgctacgcc cgtgaaaaca acgtgccata cttgggcatc tgcctcggta tgcagattgc
    10981 gctgattgaa tacgcccgcg acgtggcagg tttgaaaggt gcgaattcca ctgagtttga
    11041 cttgaaatgc gctgcccccg tcgtcgccct gattgacgaa tggcaaaccg ccgacggcag
    11101 cgtcgaaacc cgtgacgaat ccgccgattt gggcggcacg atgcgtttgg gcgcgcaaga
    11161 agtcgaattg aaagcaggca gcctcgccgt caaaatctac ggcagcggac acattcgcga
    11221 acgccaccgc caccgctacg aagtcaacaa caactacgtt tccgcgctgg aacaggcagg
    11281 tttggtcatc ggcggcgtat ccgccggacg cgaacgcttg gtcgaaacca tcgaactgcc
    11341 gaaccatcct tggttcttcg cctgccagtt ccatcccgag ttcacgtcca acccgcgcaa
    11401 agggcatcct ttgttcaccg cgtttgtcaa agccgcgttg aacaataaaa aagcctgata
    11461 aagcgttact tgatgatcaa aatgccgtct gaaagcctga caaagacttt cagacggcat
    11521 tttcgcaaat cggggatcat aaaagccatc aatttaatt
//
LOCUS       DAXFZR010000071        11072 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000071 DAXFZR010000000
VERSION     DAXFZR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.072"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            184..2557
                     /locus_tag="ACFJAS_001677"
                     /pseudo
     CDS             184..2557
                     /locus_tag="ACFJAS_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            complement(2766..3239)
                     /locus_tag="ACFJAS_001678"
     CDS             complement(2766..3239)
                     /locus_tag="ACFJAS_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC3982870.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGVGKSDSEACEHAL
                     INAARKFQQTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(3370..4725)
                     /gene="yegQ"
                     /locus_tag="ACFJAS_001679"
     CDS             complement(3370..4725)
                     /gene="yegQ"
                     /locus_tag="ACFJAS_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC3982871.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            5050..5838
                     /locus_tag="ACFJAS_001680"
     CDS             5050..5838
                     /locus_tag="ACFJAS_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC3982872.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(5884..6483)
                     /locus_tag="ACFJAS_001681"
     CDS             complement(5884..6483)
                     /locus_tag="ACFJAS_001681"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC3982873.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            6611..7228
                     /gene="gmk"
                     /locus_tag="ACFJAS_001682"
     CDS             6611..7228
                     /gene="gmk"
                     /locus_tag="ACFJAS_001682"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC3982874.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            7286..7492
                     /gene="rpoZ"
                     /locus_tag="ACFJAS_001683"
     CDS             7286..7492
                     /gene="rpoZ"
                     /locus_tag="ACFJAS_001683"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC3982875.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            7585..9741
                     /locus_tag="ACFJAS_001684"
     CDS             7585..9741
                     /locus_tag="ACFJAS_001684"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC3982876.1"
                     /translation="MPAPQPSAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGDPYITHPIAVATQLALWHMDTQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGTLHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTREMDAVAEGGIVGHWSYKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(9808..10992)
                     /gene="coaBC"
                     /locus_tag="ACFJAS_001685"
     CDS             complement(9808..10992)
                     /gene="coaBC"
                     /locus_tag="ACFJAS_001685"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC3982877.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT         2684 a         2669 c         2995 g         2724 t
ORIGIN      
        1 gtacagaccc ttaaaaataa tgaaacaaac aaggcctcgg catagatgtt tgcagagacc
       61 tttaattaca cgacacagat ttatttgtac gggttgctgt cttattaaat attaatgatt
      121 atcagaaaat ttattatgaa ttaactgatg gatgaacaat ccattacatc ttgagttgat
      181 aatatgaaac cattacacat gcttcctatt gccgcgctgg tcggcagtat tttcggcaat
      241 ccggtcttgg cagcggatga agctgcaacc gaaaccacac ccgttaaagc agagataaaa
      301 gaagtgcgcg ttaaagacca gcttaatgcg cctgcaaccg tggaacgtgt caacctcgac
      361 cgcattcaac aggaaatgat acgcgacaac aaagacttgg tgcgttactc caccgacgtc
      421 ggcttgagcg atagcggccg ccatcaaaaa ggctttgctg tgcgcggcgt ggaaggcaac
      481 cgtgtcggtg tcagcattga cggcgtgagc ctgcctgatt cggaagaaaa ctcactgtat
      541 gcacgttatg gcaacttcaa cagctcgcgc ctgtctatcg accccgaact cgtgcgcaac
      601 atcgaaatcg cgaagggcgc tgactctttc aataccggta gcggcgcatt gggtggcggc
      661 gtgaattacc aaaccctgca aggccatgat ttgctgttgg acgacaggca attcggcgtg
      721 atgatgaaaa acggttacag cagccgcaac cgcgaatgga caaatacact cggtttcggt
      781 gtgagcaacg accgcgtgga tgccgctttg ctgtattcgc aacgtcgcgg tcatgagacc
      841 gaaagcgcgg gcgagcgtgg ctatccggta gagggtgctg gcagcggaga gaatatccgt
      901 ggttcggcac gcggtatccc tgatccgtcc aaacacaaat accacaactt cttgggtaag
      961 attgcttatc aaatcaacga caagcaccgc atcggcgcat cgtttaacgg ccagcagggg
     1021 cataattaca cgatttgaag agtcttataa cctgaccgct tcttcctggc gcgaagccga
     1081 tgacgtaaac agacggcgca atgccaacct cttttacgaa tggacgcctg attcaaattg
     1141 gttgtcgtct ttgaaggcgg acttcgatta tcagaaaacc aaagtggcgg cggttaacaa
     1201 caaaggctcg ttcccgatgg attattccac ctggacgcgc aactataaat cagaaggatt
     1261 tggacgaaat atacaaccgc agcatggaca cccgattcaa acgttttact ttgcgtatgg
     1321 acagccaacc gttgcaactg ggcggccaac atcgcttgtc gtttaaaact ttcgccagtc
     1381 ggcgtgagtt tgaaaactta aaccgcgacg attattactt cagcggacag atatcccgta
     1441 ctaccagctc gattcaacac cctgtgaaaa ccactaatta tggtttctca ctgtctgatc
     1501 aaatccaatg gaacgacgtg ttcagcagac gtgcagatat ccgttacgat cataccaaaa
     1561 tgacgcctca ggaattgaat gccgagtgtc atgcttgtga caaaacaccg cctgcagcca
     1621 atacttataa aggctggagc ggatttgtcg gtttggcggc gcaactgaat caggcttggc
     1681 atgtcggtta cgacattact tccggctacc gtgtccccaa tgcgtccgaa gtgtatttca
     1741 cttataacca cggttcgggt aattggctgc ccaatcccaa cctgaaagcc gagcgcagca
     1801 ccacccacac cctgtctctg caaggccgca gcgaaaaagg tactttggat gccaacctgt
     1861 atcaaaacaa ttaccgcaac ttcttgtctg aagagcagaa gctgaccacc agcggcgatg
     1921 tcggctgtac tcagatgaat tactactacg gtatgtgtag caatccttat tccgaaaaac
     1981 cggaatggca gatgcaaaat atcgataagg cccgaatccg tggtcttgag ctgacaggcc
     2041 gtctgaatgt ggacaaaata gcgtcttttg ttcctgaggg ctggaaattg ttcggctcgc
     2101 tgggttatgc gaaaagcaaa ctgtcgggcg acaacagcct gctgtccaca cagccgccga
     2161 aagtgattgc cggtgtcgac tacgaagccc gagcgaaaaa tggggtgtgt tctcccgcct
     2221 gacttatctg ggtgcgaaaa aggccaaaga cgcgcaatac accgtttatg aaaacaaggg
     2281 ccggggtacg cctttgcaga aaaaggtaaa agattacccg tggctgaaca agtcggctta
     2341 tgtgtttgat atgtacggct tctacaaact ggctaaaaac ctgactttgc gtgcaggcgt
     2401 atataatgtg ttcaaccgca aatacaccac ttgggattcc ctgcgcggtt tgtatagcta
     2461 cagcaccacc aacgcggtcg accgagatgg caaaggctta gaccgctacc gcgcctcagg
     2521 ccgtaattac gccgtatcgc tggaatggaa gttttaatct agtattattg gattaattgc
     2581 ctcgttgggg attaaagctg tccgaattgt tttcaggaac tcattcggac ggctttttac
     2641 cgtaaatctg tgtgatgggg ttttatattg atacagtatc cgaactcagg gcagggagta
     2701 atgttgcagg attaaaaatg ccgtctgaaa aatgtttcag acggcatttt ttgattgccg
     2761 atatgttaac ggacgacatt ggcgcgcatg accgcgcgga catggaacga acctgcctga
     2821 cagtcgtatt gtccgccttg cagcgattgc ttgccaaagt agctgtggaa gccggtaaca
     2881 ctgcgcccgc cgagtttgcc ggcggtctgc tggaatttgc gggcggcatt gattagggcg
     2941 tgttcgcagg cctcggagtc ggatttgcca acgccgtttg ccgcttgccg tgtaactaga
     3001 ttcctgccgt ttgcaaccgg tacgccgccg ccgaagctga cgggaatccc tgattccaat
     3061 acgccggcgg cggtggctgc atttattgcc gcctgaccgt tgctacaacg gtaaatgatg
     3121 tttttgccgc ctcctgcggt gtctccgcgg ataactttgc atgattctcc ggccggtgtc
     3181 ccggcggatg ccgtgtgcgc gtttttaagc gcgcggccgg attggcaggc tgccagcatc
     3241 agggcggcgg cggagagtat gaggattttt tatgtcggat gtccttgttt gctgtgggaa
     3301 atcattgcca ttgtacagta agattcaaca aaatgccgtc tgaaaacggg gttcggacgg
     3361 cattgtggct caggggttca acacgcgtgc gatgagggct ttttctttgc cctgcatatt
     3421 ggggattttg acctgaatgc cgttgcccgg ggcaacatcg acaggttggc ctttgcgggt
     3481 catttgttcc aatttgatgg tttggttgcc gctcgggtgg atgatttcga gcgaatcgcc
     3541 gacggcgaag cggtttttaa cttcgatggt cgcccagcca ttttcgtcga tttcggtaac
     3601 gtgtccgacg tattggcttt gtttggcggt ggaatggccg ctgaggtagt tttgataatc
     3661 ctgagtttgg tggcgttcga ggaagccgct ggtgtagcct cggttggcaa ggccttcgag
     3721 ttcgctcaac aggctgtaat caaacggacg gcctgcgacg gcatcgtgaa tcgctttgcg
     3781 gtaggactgg gcgacgcggg cgacgtagta gagagacttg gtacggcctt cgactttgag
     3841 gctgtccaca ccgattttgg cgagtttttc gacgacttcg ataccgcgaa gatctttgga
     3901 gttcatgatg taggtgccgt gttcgtcttc cataatcggc atcatctcgc ccgggcggtt
     3961 ggattcttca atcaggaaaa ctttgtcggc gtagggatgg cgtttttgac cgttgatgcc
     4021 ttcaaagttt tggttggctt cttcttgggc tttttcaaag ttgaaacctt gcagaagctg
     4081 ggcatcgcct gcctcgcttt ccgtggcgtt gtggactttg tagtcccaac ggcaggagtt
     4141 ggtgcaggtg ccttggttgg gatcgcggtg gttgaaatag cccgacaaca ggcagcggcc
     4201 tgagtaggcg atgcacaatg cgccgtggat gaagacttcg agttcgatgt cggggcattc
     4261 ttggcggatt tcggcaattt cttccatgct caattcgcgt gacaggatga tgcgttcgac
     4321 accaatgttc tgccaaaatt tcacgcccca gtagttggtg gtgttcgcct gtacggacag
     4381 atggatcggc atttccggcc atttttcgcg cacggtcata atcaaacccg gatccgccat
     4441 aatcagcgcg tcgggtttca tggcaatcag cggctccatg tcggcaacga aggttttgag
     4501 tttggaattg tgcggcaggg tgttgacggt caaaaagaat tttttgttgc gttcgtgcgc
     4561 ttctttaatg ccttgctcga ggacgtcgag tttggcaaat tcgttgttgc gggcgcgcag
     4621 ggagtaacgc gggctgccgg cgtaaacggc atctgcgccg taatcgtagg cggcgcgcat
     4681 tctttccaat ccgccggcgg gcaataagag ttcgggtgct ttcatcgtgt ttccttttcg
     4741 gttgaaaccc cgccctttag ggcggcagga tcagacttta tttgggaggg gcgtaacccc
     4801 ttccgaatca ggacgacata tagggcggtg ctttatgtgt cgtcctgtgt gttgaagcat
     4861 gatttgtatg ctttgtaaaa atgtttcaga cggcattgct gcaaatgccg tctgaagggt
     4921 gtttgaagat gggcggatta tccgcctttt cgtgttcgcg gtcaattttg ccgggaaatt
     4981 tataaggatt tatcaagtat ttgccttctt gggatacaat gcggcagttt gagtgcgaga
     5041 gagaatatga tgaatcccaa aatcgtgttt ttcgacattg acgatacgct gtaccgcaaa
     5101 tatacggata ccttgcgccc ttccgtgaaa acggcagtgg cggctttgcg cggcaaaggt
     5161 atattgacgg cgttggcaac ggggcggtct ttggcgacga ttcccgaaaa ggtcagggac
     5221 atgatggcgg aaacgggtat ggatgccgtg gtaacgataa acggacagtt tgcgctgctg
     5281 cacggtaaaa ctgtgcgcga agtaccgatg gataccggtt tgatgggcag ggtttgtgcg
     5341 catttggatg gcttgggcat ggattatgcg tttgtcggcg gagaggggat cgccgtgtcc
     5401 gcgctgtcgg aatgcgtgtg ccgcgccttg cagcatatcg ccagcgattt ttttgccgat
     5461 aaggattatt tttcaagcaa accggtgtat cagatgctgg tgtttgcgga ggaaaacgaa
     5521 atgccgcttt ggtcggatat tgtggaacgg gaaggcttga aaacggtgcg ctggcacgag
     5581 gaagcggtcg atctgctgcc tgcgggaatg tcgaaaacag acggcatcag aagcgtggtt
     5641 gaagcattgg gtttggaaat ggcagacgtg atggcgttcg gcgacggttt gaacgatgtg
     5701 gaaatgctgt ctgaagtcgg gttcggcgtg gcaatgggta acggggaaca ggcagcgaaa
     5761 gaagcggcga aatatgtttg ccccggcgtt gatgaagacg gcgtgttgag gggcttgcaa
     5821 gatttgggtg tgatttgaac gtataataac aaccctgccg gtttcagacg gcagggtcgg
     5881 ttttcagcct ttcatgcagc cttcgttttg aagcagggta aataagggcg cgccacttgc
     5941 gcggatattc ttgccgcctt gaaggtcggt aaattccaaa atggcggcgg cttcgacaat
     6001 ttccccgccg agtttgcgga tcagttccag cccggcaagc attgtgccgc ccgtggcaac
     6061 caaatcatcg accagcagga cgcgcgaacc gggtttgacg gcatcggtgt ggatttccac
     6121 cgcagcttcc ccgtattcga gcgcgtagct ttgcgatacg gtttcaaaag gcagcttgcc
     6181 ttttttgcgg atggggacga agccgacgtt gagctggtag gcgagtgccg cgccgataat
     6241 gaagccgcgc gcgtccaagc cggcaacgat gtcgattttc tgatccatat agcggtaaac
     6301 cagcaaatcg accaaaaggc ggaagtattc cgcactttgc aggacgggcg tgatgtcgtg
     6361 gaataagatg cctttttgcg gccagttttc gattttgcgg attttgtcgg caagcgcgcc
     6421 gacactcata gcttcgggat gaaccagcat tgcgtgttcc aagtttgatg tttttaacgc
     6481 catattgtag ccgtcagcct gccgcttgcc aaatcaatca aaacaatagc ggcaaaatat
     6541 gggcatcggc aaatatgaaa tagggtgtcc gttgtgatac aatcggttgg aaaatataca
     6601 ggtaaaaaat atgtccgctt atcgaaaagg caacatcttt atcatttcgg ccgcttccgg
     6661 cacgggcaaa accacgctgg tgtcgcggct gttggcaaac cataacggtt tgcgcgtttc
     6721 cgtgtcgcat acgacgcgcc cgccgcgtga aggcgaagca aacggcgtac attatcactt
     6781 tgtttccaaa gaagagtttg aatcgcttat cgcgcaggag gcttttttgg aatacgcaga
     6841 cgtattcggc aactattacg gcacaagcac ggagggcgtg aatgcgttgg cggcggcagg
     6901 ctatgacgtg attttggaaa tcgacgttca gggcgcggcg caggttcgca acgcgctgcc
     6961 ggaagctgtc ggtatcttta tcctgccgcc ttctttcgac gtgcttgccg cgcgcctcaa
     7021 agggcgcggg acggacagtc gggaagttat ccaaaggagg ctgtcgaagg caaggcatga
     7081 aatcgaacag tccgtattgt tcgactttgt cgtggtcaat gacgacctgg agaaggcgga
     7141 gggggatttg cttcatattg tgaatgcctg ccgtctgaaa aggtcgcggc aactgggatt
     7201 tattgcagat ttgttggaaa attcctaaaa accgcgaaaa tatccggttt cccaatttaa
     7261 atatttttga aagaaagcaa ataatatggc acgtattacc accgaagact gtaccggaaa
     7321 aatttccaac cattttgacc tgacattggt agcggcccgt cgggcccgcc agcttgaaaa
     7381 tggcaatacg ccgcttgtgg acgatgtccg caataacaaa ccgaccgtta ccgccttaag
     7441 ggaaatcgca gccgggcata tcggtacgga actgttgacg cgcaataaat aaattctgcc
     7501 ggaaacgcac gccggcacac tctgccgccg tgcagtccga cggtttgaaa tgaaaaaaca
     7561 catacatccc gaaagccgtc tgaaatgccc gccccccaac cttccgcccc ttacgacccc
     7621 ctgaccgccg aagcgcgtac cctgcttttc cataccgcct cctacctcaa gcccgaggaa
     7681 caggcggagc ttgaaaaagc tgtcgcctat gcgtttcgcg cccacgacgg gcaaacccgc
     7741 aaaagcgggg atccctacat cacgcatccg attgccgttg cgacgcagct cgccctttgg
     7801 catatggaca cacagggtct ttgtgcaggc gtgatgcacg acgtattgga agatacgggc
     7861 gtgacaaaag gggaaatggc ggcggtgttc ggcaatactg ttgccgagat ggtggacggg
     7921 ctgtccaaac tcgaaaaact caaatttgaa gatcatgcgg aacatcaggc ggagagtttc
     7981 cgcaaactga ttttggcgat gaccaaagat gtgcgcgtca tcgttgtcaa acttgccgac
     8041 cgcctgcaca atatgcggac gctcggttcg atgcgcccgg acaaacgccg ccggattgca
     8101 agggaaaccc ttgaaatcta cgcacagatt gccaaccgca taggtctgaa taatgcgtat
     8161 cgggagcttc aggatttatc gttccaaaac ctgcatccca accgctacga gactttaaaa
     8221 aaagcgatgg acaagagccg gaagaacagg caggacgttg tcggcaaagt tttgcgcgcg
     8281 ttcggccagc ggctggtagg cgcgaatata gaggccaaaa tcaaaggcag ggaaaaaaac
     8341 ctgtacggca tccatcagaa aatgatggcg aaaaagctgc gctttgccga ggttatggat
     8401 atttacggtt tccgcgtcat tgtcaacagc attcccgcct gttatgccgc actcggcaca
     8461 ttgcacaccc tctatcagcc caagcccgga cggttcaaag actatatcgc cattccgaaa
     8521 agcaacggtt atcaaagtct gcatacgact ttggtcggcc cctacggctt gccgattgaa
     8581 gttcagatac gcacgcggga aatggatgcc gtggcggaag gcggtattgt cggccattgg
     8641 agctataaat cagattccaa aacggtcgat caggcagtgc tgcacaccaa tcaatggttg
     8701 aaaaacattt tagacttgca ggcaagcagc gccaatgcca ttgagtttct cgaacacgtc
     8761 aaagtcgatt tgtttccgaa cgaaatctac atccttactc cgaaaggaaa aatcctaact
     8821 ttgcccaaag gggcaacgcc tgtcgatttt gcttatgcgg tgcataccga cattgggcat
     8881 aaaacagttg ccgcgcgtgt caataatgtg atgatgccgc tgcgtacgaa gctcaaaacc
     8941 ggtgattctg ttgaaattat cacatccgaa cacgccaaac ccaatcccgc gtggttgaat
     9001 ttcgccgtgt ccggcagggc gcgcagtgcc atacgccaat atattaaaaa tcttaaccgg
     9061 cacgatgcgg tcgttttggg agagagcctc ttacaaaaag ccttgtccag tttgctgccc
     9121 aaagatgtcc tgctttcaga cggcatcaaa gaaaaatacc ttgccgatct caacgacaaa
     9181 cagacttcat ttgaggaagt gctgtacaac gtcggtatgg ggcataccct gcctgtttat
     9241 gtcgccatgc atatcgccga attggcaggg gagcatttcg gcagcgaggt caggctcagt
     9301 tccattaaag ttgatggtca ggaaagcggg catattcatt ttgcagagtg ctgccaccct
     9361 gttcccggcg attccatccg tttgctgttg gttaagggaa aaggcatgat tatccatagg
     9421 gatacctgcc cgacgttgtt gaagtccgat cccgaacagc agctggatgc agactgggaa
     9481 aatatgaacg ggcagaacta ccgtgtcggg cttcaagtcc aatcggaaga cagccacggc
     9541 ctgttggcat taatggcgca agcgatttcc gattccggtg cggacatcga gtcggtcgaa
     9601 acaccgtcta aatcccagtc gggaacggaa ggttttgtcg aattcaaatt cttattgaaa
     9661 gtcaagaatt tggatcagtt gaatcagatt attcaaaatc tgcacagcat cccatatatc
     9721 cgcaaagtca tcagaagttg aagcaggtaa aacgggttgc ctgttaatca aaaggcaacc
     9781 cgttttatgg tttatccgtt caattgctta tttgttcaaa tatacggcaa gcctttcaac
     9841 aatccgcatt gccgcttcat ctttacttgt ttcggggaaa gacaactccg catcatcatc
     9901 tatgataaca atttggttgg ttgtcttgcc catcgcgatt gaaacatcat tggcaactat
     9961 caccggtatt tttttcttaa tacgtttttc ccgcgcatat gccattacat tctccgtttc
    10021 agcggcaaaa ccgatgcaga acggcgggtt cggcaatgag gcaatagaag ccaaagtatc
    10081 ggggttctca tccaattcga tggataacgg tttggcattt ttatcttttt tgaatttttg
    10141 cgtactcctg tttttaacct tatagtctga gacggcggca acagaaataa aagcatcttg
    10201 tttttcaatc aaacgatgta ctgcgcgatg catatcttcg gcactgacgg cttgaaccgt
    10261 atcggatatg ccgaaaggca gcgtggtttg aagctgtccg tgaatcaggc tgatttccgc
    10321 accggcggca cggcacgccc gcgccaaagc tacgcccatt tttccgctgg aggtatttgt
    10381 gatgcccctg acagggtcaa tggcttcaaa tgtcgcacct gcggtaatca agattttctt
    10441 gtcccttaaa attttcggtg tccacaagtc tggaagcaaa tccagcaatt cggcaggttc
    10501 cggcatcctt cccataccat tttctctgca agcctgttcg cccaagcccg gcatatagac
    10561 agtaatgccg tctgaaacca gttgtgcgat attccgttgg ttggcaggat tcagccacat
    10621 ttccacattc atcgcgggag cgatggcaag agggcatttc cgtgcggctg ccagattggt
    10681 cagtaggtta tctgccacgc cgttacagat ttttgccacg gtattcatac ttgccggcgc
    10741 aatcagaaaa acatccgcat tccgggtcag gttgatatgt tccataccgt ttgaaccgtt
    10801 gccgccgtgc gtgtcggtca gaacgggatt gccgctcaaa gcctgaaaag tcagcggaga
    10861 aacaaattca gttgccgagc ggctcataac caccgtaacc gaatgcccct gttttttcag
    10921 cagtcgcacc aactcgcaag acttatacgc cgcaatactg cccgttacac ctaaaagaat
    10981 atgtttgccc attttgacaa tttctaatta cgaagaaaaa acagcagcca ttctataaca
    11041 aagtacggat acattagtta ccgtttggat ac
//
LOCUS       DAXFZR010000072        10979 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000072 DAXFZR010000000
VERSION     DAXFZR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.073"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(25..1053)
                     /gene="trpD"
                     /locus_tag="ACFJAS_001686"
     CDS             complement(25..1053)
                     /gene="trpD"
                     /locus_tag="ACFJAS_001686"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC3982878.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(1117..1707)
                     /locus_tag="ACFJAS_001687"
     CDS             complement(1117..1707)
                     /locus_tag="ACFJAS_001687"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC3982879.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            2118..4394
                     /gene="znuD"
                     /locus_tag="ACFJAS_001688"
                     /gene_synonym="tdfJ"
     CDS             2118..4394
                     /gene="znuD"
                     /locus_tag="ACFJAS_001688"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC3982880.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(4934..5713)
                     /locus_tag="ACFJAS_001689"
     CDS             complement(4934..5713)
                     /locus_tag="ACFJAS_001689"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC3982881.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            5867..8716
                     /gene="uvrA"
                     /locus_tag="ACFJAS_001690"
     CDS             5867..8716
                     /gene="uvrA"
                     /locus_tag="ACFJAS_001690"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC3982882.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPGRMLVL
                     KGARGNNLKNITLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEQVAKIKGSYTGKYLKVVLK"
     gene            8734..9375
                     /locus_tag="ACFJAS_001691"
     CDS             8734..9375
                     /locus_tag="ACFJAS_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HFC3982883.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSDMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            9379..10512
                     /locus_tag="ACFJAS_001692"
     CDS             9379..10512
                     /locus_tag="ACFJAS_001692"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3982884.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
BASE COUNT         2902 a         3083 c         2547 g         2447 t
ORIGIN      
        1 ttgctttata tcaagtttgc cggcttaggc gaattgccgt gtaaaaccga caaactcctc
       61 ttttttcgcc ttcgccctac ctgaatcgat gccttcccgt gcggcagata tgccgtctga
      121 aagcgaagca gcgatatttc cggcatataa ggcggcggcg gtattaagca atacgatatc
      181 gcgcgccgcc ccttctttcc cgtccagtac ctcgttcatt ttcaacaaag attcctgagt
      241 attggcaact ttgatttcat ccaaattgcg gcgggtttcg ataccgaaat cttctgggcg
      301 gatgtcgtat tcgctgattt ttccgtcttt gagttcggca acgcgcgttt tgcccgtcag
      361 tgtaatttca tccaaaccgc cctccccgca aacgaccaaa acgtgttttg agccgagttg
      421 ttgcaagacc cgcgacaaaa tgccgcacaa atcggtgtgg aacacgccca aaagctggtt
      481 cggcgcgccc gcaggattgg ttaacggacc caatatgttg aaaatgcttc ggaaaccgag
      541 cgaacggcgc accggggcga catggcgcat ggcactgtgg tgattgggtg cgaacataaa
      601 ccctataccg gtctgcctga tactttgggc aatctgttcg ggagtcaggt tgaggtttgc
      661 gcccatttgc tccatcacgt cagccgcacc gctggaggaa gagaccgacc ggcctccgtg
      721 tttggcaacc tttgcgcctg ccgctgcagc aacaaacatc gaagtcgtcg aaatattgaa
      781 ggttttcgcg ccatccccgc ccgtaccgac gatatcgacc agcccgtccg catcctccag
      841 cggcactttt gacgcaaact cgcacatcac ggcggcggcg gcggtgattt cggaaacggt
      901 ttcaaccttg atacgcaagc cggtcaaaat cgccgcaatt tgttcgggcg gcacttttcc
      961 gctcatcatt tggcgcatca agtcggtcat ttcatcgtaa aacaactcgt tattgctgat
     1021 taatcgttcg atagcctgtt gcggtgtaat cattttttgt cctccgttca atattcggac
     1081 gaaaatgccg cccgaagggc ttcagacggc atcacgtcag attttttgcg gtttgaagtt
     1141 ttgaaattca actaaaaaat tgttcaacat atcatgtccg cgttcggtca agagggcttc
     1201 ggggtggaac tgcacgccct cgacggcata ttccttatgg cgcacgccca taatttcgcc
     1261 gtcttccgtc cacgccgtga tttccaagca atccggcagc gtgccgcgtt cgataacgag
     1321 gctgtgataa cgcgtacagg taaccggatt gggcaaaccc ttaaacatac ccttgcccga
     1381 atgggacacg ggcgacacct taccgtgcat caaggttttt gcccgtacca catctccacc
     1441 gaacgcttcg cctatcgtct gatgcccgag gcacacgccc ataatcggca gccggccggc
     1501 aaaatggcgc atggcttcta ctgaaatacc cgcctcctta ggggaacacg gtccgggacc
     1561 gataacgaga tattgcggat tcaatgcctc gatttcctcc aacgtaatat catcgttgcg
     1621 gcgcacggcg acttcctgcc ccaattctgc gaaatactgg acgatgttgt aagtaaaact
     1681 gtcgtaattg tcgataaaca aaagcatttt gtgattaacc tattgattta taatttgttt
     1741 attattaact gcatttccga tacccccatc cataccccct tatatcttag cgtgcccgat
     1801 gcgccctcgt gaatctggcg cagagcctcg cgttctgaca agtacgccat tttgccacct
     1861 tttcgaccgt tcgggaacgt accgttttta tgccctcata aattttggtt atccgatagc
     1921 taacatatag cggcgttcgt gttcacatca ttaacttccc taaaattagg gttaatacaa
     1981 attaacgcca ttaaatatca caaccgtgtc atttgaaaaa cttttgccta tgccctctga
     2041 gtattctcaa aatcttatga tataaaaaat gcaatgttat ataataacaa acttttgaaa
     2101 tgcgacgaga agccaaaatg gcacaaatca cactcaaacc cattgtttta tcaattcttt
     2161 taatcaacac acccctcctc gcccaagcgc atgaaactga gcaatcggtg ggcttggaaa
     2221 cggtcagcgt cgtcggcaaa agccgtccgc gcgcgacttc ggggctgctg cacacttcga
     2281 ccgcctccga caaaatcatc tccggcgata ctttgcgcca aaaagccgtc aacttgggcg
     2341 acgctttgga cggcgtaccg ggcattcatg cctcgcaata cggcggcggc gcatccgctc
     2401 ccgttattcg cggtcaaacg ggcagacgga ttaaagtatt gaaccatcac ggcgaaacgg
     2461 gcgatatggc ggacttttct cccgatcacg ccattatggt agataccgcc ttgtcgcaac
     2521 aggttgaaat cctgcgcggg ccggttacgc tcttgtacag ctcgggcaat gtggcggggc
     2581 tggtcgatgt tgccgatgga aaaatccccg aaaaaatgcc tgaaaacggc gtatcgggcg
     2641 aagccggatt gcgtttgagc agcggcaatt tagaaaaact gacatccgca ggcatcaata
     2701 tcggactggg caaaaacttc gtgctgcata ccgaaggctt gtaccgcaaa tcgggcgatt
     2761 acgccgtacc gcgttaccgc aatctgaaac gcctgcccga cagccatgcc gattcgcaaa
     2821 cgggcagcat cgggctgtct tgggtgggcg aaaaaggctt tatcggcgca gcatacagcg
     2881 accgtcgcga ccgctacggc ctgcctgccc acagccacga atacgatgat tgccacgccg
     2941 acatcatctg gcaaaagagt ttgatcaaca aacgctattt gcagctttat ccgcacttgt
     3001 tgaccgaaga agacatcgat tacgacaatc cgggcttgag ctgcggcttc cacgacggcg
     3061 acggtgcaca cgcacacacc cacaacggca aaccgtggat agacctgcgc aacaaacgct
     3121 acgaactccg cgccgaatgg aagcagccat tccccggttt tgaagccctg cgcgtacatc
     3181 tgaaccgcaa tgactaccac cacgacgaaa aagcaggcga tgcagtagaa aacttcttca
     3241 acaacaaaac acacaacgcc cgtatcgagt tgcgccacca acccataggc cgtctgaaag
     3301 gcagctgggg cgtgcaatat ttgggacaaa aatccagcgc actttccgcc attcccgaaa
     3361 ccgtccaaca accgatgttg attgacaaca atgtccgcca ttacagcttt ttcggtgtag
     3421 aacaggcaaa ttgggacaac ttcacgcttg aaggcggcgt acgcgtggaa aaacaaaaag
     3481 cctccatccg gtacgacaaa gcattgattg atcgagaaaa ctactacaac cagcccctgc
     3541 ccgacctcgg cgcgcaccgc caaaccgccc gctcgttcgc actttcgggc aactggtatt
     3601 tcacgccaca ccacaaactc agcctgaccg cctcccatca ggaacgcctg ccgtcaacgc
     3661 aagaactgta cgcacacggc aagcacgtcg ccaccaacac ctttgaagtc ggcaacaaac
     3721 acctcaacaa agagcgttcc aacaatatcg aactcgcgct gggctacgaa ggcgaccgct
     3781 ggcaatacaa tctggcagcc taccgcaacc gattcggcaa ctacatttac gcccaaacct
     3841 taaacgacgg acgcggcccc aaatccatcg aagacgacag cgaaatgaag ctcgtgcgct
     3901 acaaccaatc cggtgccgac ttctacggcg cggaaggcga aatctacttc aaaccgacac
     3961 cgcgctaccg catcggcgtt tccggcgact atgtacgagg ccgtctgaaa aacctgccgt
     4021 ccctacccgg cagggaagat ccctacggca aacgtccttt catcgcgcag gacgaccaaa
     4081 acgccccccg cattccggct gcgcgcctcg gcttccacct gaaaacctcg ctaaccgacc
     4141 gtatcgatgc caatttggac tactaccgcg tgttcgccca aaacaaactc gcccgctacg
     4201 aaacgcgtac gcccggacac catatgctca acctcggtgc aaactaccgc cgcaatacgc
     4261 gctatggcga gtggaattgg tacgtcaaag ccgacaacct gctcaaccaa tccgtttacg
     4321 cccacagcag cttcctctct gatacgccgc aaatgggccg cagctttacc ggcggcgtaa
     4381 acgtgaagtt ttaaaatcgg acaggcaaac aaaaaaggtt ttcagacggc attctgaaaa
     4441 cctttttttc tttttaatca acttgttagg aaatatggca aagacaagga acaaaccact
     4501 gtgccatcat tcccattttc tttgtttcat cattcttgct ttcacgccgt cattctcgca
     4561 aaagcagaaa aatcaaaagc aaaaacccaa gatcccatca ttcctgcgca ggcgggaatc
     4621 caattcattc ggtttcagcc gtttccgata aattaccgta acgttaagtt cccggattcc
     4681 cactttcgtg ggaatgacgg cgtaaaggtt tctgtttttt aagttttagg taactaatga
     4741 gtcgtcattc ccgcgcaggc gggaatccaa ttcgttcggt ttctgttatt tctgataaat
     4801 caccgtaacg ttaagttccc ggattcccat tttcgtgaac ataacggtgt gaacattagc
     4861 aaaaattcaa aaaaccaaac ttcctcattc gtgtgaatga tggtactgat attttctatt
     4921 tcagttcgca aaattaatct ttcaacacat cttgcacagc cgcctgaatc ttagccgccg
     4981 ccgcttcgat ttgctgctgt tcggcagatg ggtttgccgc tgtttcaacc gaagcagctt
     5041 gtgaaacagg atcggcttga gaatcagtca gcggcaaacg cgccaatacg gtttttacgc
     5101 cggttacttt atcaccaatc gccacttgcg cctgcgcatc gacaggcaga tacatatcca
     5161 cgcgcgaacc gaagcggata aagccatagc gttcgccgcg ggacagtttc gcacctgctt
     5221 gggtgtaaca caaaatacgg cgtgccacca gaccggccac ttgaacaaaa gtaatttcac
     5281 gaccggaagc cgtagtcgcc aaaaccgcgt tgcgttcgtt ttccgtgctg gctttgtcca
     5341 aatccgcatt cacgaatttg cctttgttat agaccacttt cgttaccgta caatcggcag
     5401 gcgatttttg cgaatgcacg ttgaacacgt tcataaaaat actgattttc aaagcatcga
     5461 catcacgata cggatcgcgt gcgcgctcga ccaccacgat acggccgtca accggactca
     5521 acaccgcttc aggattttgc ggaatttcac gcgcagggtc gcggaaaaac tgcaatgcaa
     5581 atacggtaaa cacccaaaac ggcaaagacc accagccgca gcacatcgac accagcaggc
     5641 tcaaagccaa accgccgccg ataatcggcc agccctcacg ggcgataatc gggtggggat
     5701 aaagacggtt catttgtcat ccttttattc aaatatatta aatagcgaca ttataacgga
     5761 atccgccgcg ttccgccgac aaatgccgtc tgaacgcggc ttcagacggc atccgagaaa
     5821 gtgctacaat atcggttttc ctttattttt tcaggcggca tccattatgt gcaaccatca
     5881 tccccgacat tcgcacgaca acgacaccat ccgcattcgc ggcgcgcgca cgcataattt
     5941 gaaaaacatc gatttggaca ttccccgcca caagctcgtg gtggtaacag gattgtcagg
     6001 cagcggcaaa tcatcgctgg cgtttgatac tttatatgcc gaagggcagc ggcgttatgt
     6061 cgagagcctg tctgcctatg cgcggcagtt tttgcagatg atggacaaac ccgatgtcga
     6121 tttgattgaa ggcctgtcgc ctgcgatttc catcgagcag aaatccacca gccacaatcc
     6181 gcgttccacc gtcggcacgg taacggaaat ccacgactac ctgcgccttt tatacgcccg
     6241 tgtcggcacg ccgtattgcc ccgaacacaa gctgccgcta tccagccaaa ccgtatctca
     6301 aatggtcgat gccgtattaa aactgccgga agatacgcgc gtgatgattc tggggccggc
     6361 ggtgcgcgag cgtaagggcg agtttgtcga tttctttgcc gacttgcagg cgcaaggctt
     6421 tgcccgtgtg cgcgtggacg gcgaagtcta tcagcttgac gaagtaccga agctggaaaa
     6481 aaacatcaaa cacaatatcg acgtcgtcat cgaccgcgtg aaagtgaaag ccgacatcaa
     6541 acaacgactg gcggaaagtt ttgaaaccgc gctgcgccac ggcaacgagc gcgcgctggc
     6601 gatggaaatg gacagcggcg aagaacattg gttttccgcg cgttttgcct gccccgtatg
     6661 ctcatacagc ctgcccgaat tggaaccgcg cctcttttcg ttcaacaacc cgatgggttc
     6721 ctgcccgact tgcgacggct tgggcaacac caatttcttc gaccccgaaa aagtagtcgc
     6781 ccatcccgaa ttgtcgctgg caaccggcgc gattgacggt tgggacaaac gcaaccaatt
     6841 ctatttccaa atgattcaat cgctggcgca tcattataaa tttgatgtca acgtcgcttg
     6901 ggaaactttg cctgaaaaag tcaaaaaagt cgtgttgcac ggctcgggca aagaagtcat
     6961 tgatttcacc tacctgtccg aacgcggcac caccttcaac cgcagccacg ccttcgaggg
     7021 catcatcccc aatctcgaac gccgctaccg cgaaaccgac agcgaaaccg tgcgcgaaaa
     7081 actgcgcgag taccaaaatc accgtgcctg cccgagctgc ggcggcgcac gtttgcgcaa
     7141 agaagcacgc tacgtttacg tcggcggcga accgttgcac gaagtctccg cctggccgct
     7201 gaccaaaacc caccgattct tcgaaacgct ggatttggac ggcaacaaaa aacagatcgc
     7261 cgaaaaaatc ctcaaagaaa tcaccgagcg gctcggcttc ttgattaacg tcgggctgga
     7321 ttacctgaat ttaagccgtt ccgccgaaac cctttccggc ggcgaagccc agcgcatccg
     7381 cctcgccagc caaatcggca gcggcctgac cggcgtgatg tacgttttgg acgaaccctc
     7441 catcggcctg caccagcgcg acaacgaccg cctgctcgcc accctcaaac gcctgcgcga
     7501 tttgggcaac agcgtaattg tggtcgaaca cgacgaagac gccatccgcg aagccgattt
     7561 cgtcgtcgat atgggccccg gcgcgggcga acacggcggc aacgtactga ttgccgacac
     7621 acccgaaaat gtcgccaaat gcgaaaaatc cgttaccgga caatacctcg gcggtaaaaa
     7681 atccattgcc gtgccgtctg aacgcacgcc cgtcaatccc ggccgaatgc tcgtcctcaa
     7741 aggcgcgcgc ggcaacaacc tcaaaaacat aaccctcgag ttgccgcttg gtttgattac
     7801 ctgcatcacc ggcgtatccg gcagcggcaa atccaccctg attaacgaca ccctcgccaa
     7861 aatcaccgcc cgcgaactca accgcgccca agaagaaccc gccccatacg acgacatccg
     7921 cggcctcgaa cacctcgaca aagtcatcaa cgtcgaccaa tcccccatcg gccgcacccc
     7981 gcgctccaac cccgccacct acaccggcct gtttaccccc atccgcgaac tcttcgccgg
     8041 cgtacccctc tcgcgcgaac gcggctacaa cgtcggcaga ttctccttca acgtcaaagg
     8101 cggccgctgc gaagcctgcc aaggcgacgg cgtaatcaaa gtcgaaatgc acttcctgcc
     8161 cgacgtgtac gtcccctgcg aagtctgcca cggcaaacgc tacaaccgcg aaaccctcga
     8221 aatccaatac aaaggcaaaa acatcagcca agtcctcgac atgacggtcg aagaagcccg
     8281 cgaatttttc gacgccgtcc ccaccgtatc gcgcaaactg caaaccctga tggacgtagg
     8341 tctcggctac atccgcctcg gccaatccgc caccaccctc tccggcggcg aagcccagcg
     8401 cgtcaaactc gccttggaac tctccaaacg cgacaccggc agaacgctct acatcctcga
     8461 cgaacccacc accggcctgc acttcgccga catcgccctg ctgctggaag tcataggccg
     8521 tctgaaaggc aaaggcaact cgattgtgat tatcgagcat aatctggatg taattaaaac
     8581 cgcggattgg attgtggact tagggccgga aggcggcgat ggtgggggta aggttatcgc
     8641 gaaaggtagc cctgaacaag tggctaaaat taaggggagt tataccggga agtatttgaa
     8701 agtggttttg aagtaaacga acggatatag aaaatgaaca ataattttga ccgcagcaag
     8761 catatctatc ataacgaagc ttttgtagaa ctggttaaag atgccatacg cttttttcat
     8821 ggtacgcctg ttcatacact tcctccaccc aaacagttca aaggtgcagg ggtttatgcg
     8881 atttactata tcggtaacaa tcctttgtac aaacaatatg cggactggaa cagattatcc
     8941 tacaatgcgc caatttatgt tggcaaggcc gtccccaaag gctggcggca agcaagaaat
     9001 tctgataatg cgctaaacca atcaaccgaa ctgttccacc ggttgaaaga acacagcagg
     9061 agtattgccg ctgtttctga tttagatcct tccgatttca tgtgcagatt cgttatcttt
     9121 gaaggtgccg gttctgacat gataggcaca atcgaagctg ccctgattaa attacacaaa
     9181 cctctatgga actcttgtgt cgatggtttc ggaaaccacg accccggaaa aggccgttac
     9241 gaacaagcca aatcggattg ggatgtcctt catagcgggc gggtttgggc agataggcta
     9301 aacggcatac cgaatagcta tgagtccata cttgaaaata tcaacacaca tttagagata
     9361 ataaagagaa aataacctat gaagtctttg gaaatttttt ccggcgcggg cgggttggca
     9421 aaaggccttg aacttgcagg attccaacat gcatctttta ttgaactcaa taaagatgca
     9481 tgcaattctt taagaagcaa ctttaacccc aagcttgttt atcagggaga tgtggcagat
     9541 tttgatcttt ccagtcaaga aggcatagaa gtcattgcag gcggtcctcc ttgccagccc
     9601 ttttcattgg gcgggaaaca ccttgcccat gaagacagaa gggatatgtt tcctcatgcg
     9661 gtcagatatg tggagtatta ccgccccaaa gcttttatct ttgagaatgt aaaaggactt
     9721 ttaagaaaat cattcgctga ttattttgaa tatatcctac ttcggcttac ttatccgaat
     9781 cttggtattt tgcagaatga agattggaaa ggccatctga cacggctgaa agaaattgaa
     9841 ttcaatttat ataaaggaat caaatataag gtttcatatc aattgttgaa tgcagctgat
     9901 tacggagtgc cgcaaaaacg cgaacgcgtg gttatcgtag gtatccgggc cgatttggat
     9961 attgattgga aattcccaaa aagaacacat tccgaagaca ggttgaattg ggaaaagtat
    10021 gtaacggggg aatactggga aaaacacaac gaacccaaaa gattcaataa agatattgct
    10081 gaaaagttac aaaaaaaata cggtatattc gaaccagaaa aaaaaccttg gcaaacggta
    10141 agggatacct tgtccgacat cccgcatcct ttggggaatc ataaaattac aggacatgaa
    10201 tatagggatg gcgcaagaat ttatcccgga cacacaggaa gcgggataga cgaaccgtcc
    10261 aaaaccatta aagcaggtgg gcatggcgtt cccggcggag aaaatatgat tcgttatgat
    10321 gatggaacag tcagatactt taccagctat gaagcaaaac ttcttcaaac attccctgaa
    10381 gaatttgtca tttctggagc ttggggagaa gcaatgcgac aaattggcaa tgcggttcct
    10441 gtcaaattgt cggaaatttt aggcaaacat ctgatggggg tgttgtcgga gaaaagcagc
    10501 ctgcacaact aacccgccca ctgcgtgtgt gcggtacgca cgcggttgct tgattgacgg
    10561 tttgccgttt caacttttaa cttcgttgaa gctagtgctt tcagacggcc tccaatgtcg
    10621 tttggaaaca tcaggtccaa caaagttaaa ccttaaacaa caaaccattc cttctccaac
    10681 acccaaacca caaacaaaaa tcctatggcg cgttatcgca gaaacttcat tgccggcggc
    10741 acattctttt tcaccgtcaa acccgccgcc ccgaaatcgc gtctgcttgt cggacatatc
    10801 ggcttcgcgt gcggcttata tggatgtgca aaaacaatat ccctttgaaa ccgtcgccgt
    10861 atgcgtgctg ccgaaccata ttcacgccat ttggacgctg ccgcccgacg atgcggatta
    10921 ttccctactc cggcggctga ttaaaaccaa attctccgca tattcccctc atactaaaa
//
LOCUS       DAXFZR010000073        10881 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000073 DAXFZR010000000
VERSION     DAXFZR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.074"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(132..6205)
                     /locus_tag="ACFJAS_001693"
                     /pseudo
     CDS             complement(132..6205)
                     /locus_tag="ACFJAS_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            6406..6657
                     /locus_tag="ACFJAS_001694"
                     /pseudo
     CDS             6406..6657
                     /locus_tag="ACFJAS_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6655..6847)
                     /locus_tag="ACFJAS_001695"
                     /pseudo
     CDS             complement(6655..6847)
                     /locus_tag="ACFJAS_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            7241..8578
                     /gene="ribBA"
                     /locus_tag="ACFJAS_001696"
     CDS             7241..8578
                     /gene="ribBA"
                     /locus_tag="ACFJAS_001696"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC3982885.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALDRVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(8741..8857)
                     /locus_tag="ACFJAS_001697"
     CDS             complement(8741..8857)
                     /locus_tag="ACFJAS_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC3982886.1"
                     /translation="MPSESLSDGILFGLENTYAVLSPKERIQKTKATGIHRK"
     gene            complement(8889..10490)
                     /locus_tag="ACFJAS_001698"
     CDS             complement(8889..10490)
                     /locus_tag="ACFJAS_001698"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC3982887.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEDVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(10557..>10881)
                     /locus_tag="ACFJAS_001699"
     CDS             complement(10557..>10881)
                     /locus_tag="ACFJAS_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982888.1"
                     /translation="TEVQQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLD
                     QRQELLNYLAEYNNNILKFNADGSRVEISTDVQLKHLLYGIDERYYTTALGKEKRLAN
                     SVQPI"
BASE COUNT         2493 a         2660 c         2485 g         3243 t
ORIGIN      
        1 gggcaattat agcaatgcac aaaaacaaac aaaaagataa aaaaactcca taaatgaaaa
       61 tttaatcaca tgtttatagg atatgaaaaa gaggaagcga aatctgaagc cgtctgaaaa
      121 caaccccaca cagcatcggc ggacgagttt ccctcagctg ctccggcagc cgtacccagc
      181 atccttgtaa tcgccgaaac ggtctctttt tcctccgcat tcaggctgcc gccgtctcct
      241 ttgccgtaca gccatttgcc gatgattggg gcggccgctt ccgacccgcc cgcacccaat
      301 gctcctgccg gggcattgtt gcctcatgct gcggcaaccg ctgctcctaa taccgcgtgg
      361 gcaagaacgt gagcggtttc ttgactggcg gttagtttgc cattcgcgtt ttgaccggct
      421 aaatctttaa agtgctgtcc aatcgcatac gatacgtctg gcgatgcggt agccgcagcg
      481 atgcccgctc cgctttgggt cggctcagct aaacctgagg ctaacatgtt gagaatgact
      541 ttgccttgtt gccaattatc tgcttttgct gccgcatctt gagcttcatg ggctttgcgt
      601 ttggcagttt ccatatcgcc attggctaat gcctcggctg ctgctgtttc ggctgcttct
      661 ttgtctgctt tgagtttgtc taaatgttgg ttaatctcgg tattggtttg ttgaacattt
      721 ttactaaaat cttggctgac ggttctttgt aaatccagtt cactttgcac tctatctttg
      781 tcaaatatgt ttttcaggct gcccgaatgt cgttcggcgg tgtctgtggt tacgtttgta
      841 tcaatatcgg ctttggtttg tgccgctatt ttgcctgtca gcctgatttg tgcggcttcg
      901 tctgtgattt gaatgttttt gggtattgat gccgcttttt gtgatgctgc tttgactgtc
      961 gctgtcgctg ccgtacccta ctgatgaact tgcgccgttt ttatcggcta tgcttgtcag
     1021 gtgtttgtct tgaggtttat tttttgcgcc ctgtcccagt gttttgccgc ttacggcaac
     1081 gctggcaccc aatccaaaac tttttccttc gtattggctg taattttgaa tatcactgcc
     1141 ggcgagtgtg cctgtgctga atcggttttt acccttgtct tttgcgcttt ggctgctggt
     1201 gatgatgccg cctttgaggc ctgtatggtt tccgaccttg atttgatagc cgtcttctcc
     1261 ggcataaata ccgctttgct cggttaccga agcatggtcg gctcggattt tgctttggct
     1321 gtaatcgcca ctggcactga agccataacc tacggtaact tgtgcaccgg cgttttgttg
     1381 tttgctttga taagtttctc tatcttgtac actttgaatg cttaggtttt tggcattgac
     1441 ttgtacgcct ttgccgcgta cttgcgcgcc tttgatgatg gtatcgccac cactttggat
     1501 aagggtttgg ctgcctttgt cgccaatatg gctatggcgg tgggttacgc tatcgccatt
     1561 gccgtagcct ttgccgacat tgccgcctgc ggcaacgcct aatgaccagc ctccttgtcc
     1621 gaatgatacg gcagcacctg cgttccagcc tgctgatttg ttttggctgc gttcggtatt
     1681 gctttgctcg gctgattgca gatggatttg cttatcggca atcaggccgg ttcctgctct
     1741 gcctgaaaca cctgagcctg tgatgttgat attggattgt tcgccgcacc ttcggcaata
     1801 agggtagttt tgccgcctgc ttgaatttga ctcgcttggg cttgattggc ttgaacttgg
     1861 gtggtttgtc ggttttgctg ttcgccgtag gttatggaga tgctgacttg tttggcattg
     1921 gttgtaccat tggctaagtt ttgtgcgcct ttgcctgttt gataggcctg ccaacctgca
     1981 ttggcagccg ccatggcatt aacgcggtcg tttttgcttt gtccgacttg tttgctgctt
     2041 tgtgtgctac ggcaatcgct tgttgtgcca aatcggtaac gggcgaactg aatgccaccg
     2101 ttaagccttt ttgttcgtag gtttgggtgg ttttgctgtt taattggttt tgtgctgcgc
     2161 caatatccat actttgcgtg ctgataaggt tgttgccctc agggctggaa acgttgctgc
     2221 cggtttgttc gtagtgtttg cttgcaacaa tggtggtatc gcctttcagg ctgcctacgg
     2281 tactgcctgt atgttcgttg ctttgggatt ggttttcttg tgtgtttgtc ttgctgccaa
     2341 tagtgaagcc gatacctgca ctcatcaatc ctgatttttg ggtttgatga taggtttcgc
     2401 tttggctttg agtttgggtt gtaccaatgc gaacatgatt gcctgcttga atccgggtgc
     2461 cattatcgga aataacatta ctgccaagga tgttggcatc gtttcctgcc tgcaatacaa
     2521 cttgcttgcc ttcaaaggtg ctgctttgag cagtttcgtg atgactttgg gctttatcgg
     2581 taatgactaa tttattaccg ccgccgcttc tgcctgtatg tttggacgca tcatcaactt
     2641 ggccggcatg gatgcctgag ctgatagtaa tgtcattttt agcatacacg gcaagtgtgc
     2701 cttttgcgct gccgacttcg gcagctttgg cattgagatt attccctgac aatagggtaa
     2761 catcgccttt tgtttgaatg ctgctgccga cttcgttcgt tgaacctcgg atggtatggt
     2821 tatcggcatc aaaatggatt tcttgatatt tgccggtttg taccgtatcc aggttaatgt
     2881 cgcgtcctgc ctgcagccgg gtttgccctt gatctgattg attgctgatt tgaccggcaa
     2941 tgatgttgat gtctttgcct gcctgcgctg ctaaaacacc tttttctttg cctgtgatat
     3001 aaatacctgc cattcggtct aggtaggtgc tgctaccttg tgcattttga ctgctcttgg
     3061 ccgtgctttg gttgttgatg ttgttacccg cattgagcaa taatgtctgt tcggcagaaa
     3121 gaatgccgcc aatattattg atgtcttgtg tggccgtaac cgctgatttt tgcgcatgaa
     3181 tacgcccacc gatattgtct agcgtatcgg tattgataat aagcgcattg cgccctgcaa
     3241 tcgtgcctga gtttttcagg ctgcctgaaa cattgatttg tgtattgctg cctgacaaca
     3301 atgcaccttt accgtctatg tcgccatttt taacgcgtac ataaacctgt ggcatcaata
     3361 cggtttgtgt gccgccatca ggaagtttaa cttctttttg taccaaccaa acaatatcgc
     3421 tggtcagttg cgctgcttgc tcggcactta atgcaatgcc aacgctgaga ttcatcgaac
     3481 gtgccgcagt cgcgccatta tccattaggg ctttaaattg ttcttcgtcg ttttgataac
     3541 cgtctaaacg acgatgccct gtcagctctg cgatttgttc attgattaaa cgttgctcgt
     3601 aataaccatc acccaaacgt ttatgtaaat tgtttgggtc tagtttgagg ctgcccagca
     3661 tatagtcact acccaaccat tgacggtagt tggcaaagcg tggatcggtt tcaacaagat
     3721 agcctttatt ggcaggattg ataatgtata agctgctgcc gggtaatggg gtaaaagaat
     3781 tgggcgtata gggtagcgaa ataccgttgc ttttcgcagt acggatatta tcccggttac
     3841 tttgtggcaa ctcagtgcct tggctgggcg catgacggct taatgctttg ctatgcgatt
     3901 cataggcaaa tgaacccagt gaaatgtcgc gtgtgatttc ctccggcaaa gtataatttt
     3961 gttcacgatg ccctgtttca tcatgtcctt tacgacgcgc acgccagtag ttgtgcaact
     4021 taccattttc gctgaagact ttcttctcgc caaaggtttg ctcgttatgc aaaccgtctt
     4081 tttctgtttg cacaagcaat tgccgccggc gatgattcgg ctgtcgctgt tgaagactgc
     4141 tttgctatca ataatcaaat cgctacctgc aatgatttta gcaggcgcag ttccggttac
     4201 ttgagtttct tgcgttactt tttcataatc gtatttatgc caattttcat gcgccactcc
     4261 atcaggggtg cgtaagtggt ctgattcatt gttgtagaca aaccagccta attcatgttg
     4321 cgtgccttct cgcaataatt cgtgtcgtcc aaatgcttcg taatcaacaa tacgctcgcg
     4381 ccctgtttct accaactgcg ttttcaaatg ctcattggta ttgtgcagct tttctacacc
     4441 taaacgcatt ttgcctgcag cttcaatgat tgcgccggca ttgtgtatcc tttgggcttt
     4501 gcctgtggct tggtcattgg tatctaatgc gccgccaacc gccatatcgt taccgctgta
     4561 aatcagactg ttttcacggt tatttaattg ttcaatgcct aaattcaggt tttcgcgtgc
     4621 cgcaatggcg gcacctgtac cgttttcatc ttgattgtct aagcgggtag ccgcaatagc
     4681 gatattgtcg ccataaatcc gacctgtacc gatattattc atttgcccgg cttggatttt
     4741 ggtttgttgt ccgtcaatca agcctctatt ggttaaattg tgctgcgtgc caatgtctgt
     4801 cgtaccgccg gattgaatgt tgccttgtac tgcattatca aggttatttg ctttaatccg
     4861 aatgcgtttt cctgcttgca aggtatgtga atttttcagg ctgcctcgtg tactgagcga
     4921 caattcattg cccgccacga tcttgcgttc tacataaaaa tcatcttgta acgcaatatc
     4981 cagtttatta tcagcggcaa gtgtgccgtt gttggataac gattttgcct gaatggcaac
     5041 atcacggcct gattgtatcg tgccattcgt attatcaatg acaacggtag attgctgacc
     5101 atcgtgaata atcagttgtt gattggtcgc tatttcgcca ttttgattgt tcaggctgcc
     5161 tgaaacggct aaatcctctg ttcctgccga caataatttg ccagtgtttt gaatcgattg
     5221 actgtgaatt tgagtgcctt gttgcgatac cgccgtaccg ctgttttcaa aggcttgact
     5281 gcggatattg actttgtgtt ccgctgtatt attcatatct tgcacattgg cggcagccat
     5341 cgtgccacta ttgaccaaac ggccatttgc atcaatcgcc acattaccgg aagaagcaaa
     5401 caactgccct tgattacgaa tgcctgcttg ttcggccgta ctgatcaagg tgattttgtt
     5461 ggcatacaag aatacgtgtg aaatcggtat cacgggcatc caaaccgtgt ccggcgatta
     5521 caacattgcc ttgccttatc ttaaagccgc taaagtctcc tgcttgatat tgcggttggc
     5581 ctgtcgtcaa agtggcacgg gaagcattga taaaaccacc accattgact gcaatccctg
     5641 ccggattggc aataacgact tctgcacgtc gtccacccac ttcaatatag ccattcagtt
     5701 gtgaaggatg gctgctgttg atttggttta caaccacacg tgcttcgccc cttgtcaacc
     5761 aaggattgcc ttgaatccaa ccgcctagct gtgtttgggt gttgctgcga ctgttgttta
     5821 aaatcgcccc gcgattaccc acatcaaact gggcatattg attaacagaa acccctgccg
     5881 aagtaggggt ttgaatattg acttgcggta tgccgttacc tgtttgcaga atcgtggctt
     5941 gttgggtttt aggagcagct ttatcagtaa taatgccgtc agcaaaagca atattgaccg
     6001 tacccaaagc caaacataaa gaaaagccta atgcagaaaa acaaaaggct ttggaatgag
     6061 taggaatgaa agaaacggat ttcacataaa cgctgcccga accactatcg gcacagcttt
     6121 taccttcgcg cttggtggtt tcggcaacag ctaccacagc accgcgtttg cggttgaaaa
     6181 tcacacgata gagggttttg ttcatgattt tagttatttg atttttatag agttattaca
     6241 aacattggac agtctgggca ttctaggaca aagattttgg taagtcaatt accgccattt
     6301 ttttattaaa ttaggggacg taccggataa cggctaaaaa tcccatttag gttagaataa
     6361 gccaatgaga aaagccgtct aagccggcat aaacaaaata aactcattgg gctatttgtc
     6421 gcaggcgtaa ctgcaagaac agcggcagag ttggtaggcg ttaataaaaa taccgcagcc
     6481 tatgattttc atcgtttacg atgactgatt tatcaaaacg gtccgcattt agaaatgttt
     6541 gatggcgaag tagaagcaga tgaaagttat tttggcggac aacgcaaagg taaacgcggt
     6601 cgcggtgctg ccggaaaagt cgcagtattc aggcttctca aacgaaatgg caaggtttcc
     6661 tttgccgtcc gttatgtacg tttcgtaaca ttcgaagaat tcctgaaccc tgcccgccgt
     6721 ttcggtttct gtaccccctg ttagataagg ggggaagatt cgaagcggtt gccggcttcc
     6781 tgccgtccgc cggcgcgtcc gtcatcacgc cggcaaccgc ctttgtcatc ccttgcttat
     6841 cttccatgtg caaatcctca aaaacgggca aaaaaaagcc ctgttacttg tagaaagtaa
     6901 agggcgatcc gtccggtttt tatcgggaag gcggcattca tcataggatt gccggtattg
     6961 cgcctgctcg taccgcctat gccgtacccg gcaatcaagg ctttttatca gaattttttt
     7021 cggaataacc ctccggaaac cccataatca gaagcgtgcg ggaagaaaca gccgccccgc
     7081 cggcggggtt gcggcaatgc cgtctgaagc cacgaatccg gcttcaaacg gcatctgttt
     7141 accaaaaggc aaataatccg atttggcgaa aaacagattt gctttttggt aaatacgcgc
     7201 ttacaatccg ctacatccga tttctacaaa ggataaaacg atgaccgaca cagccggctt
     7261 gcgccgccgc aacctgcggc aatggatagc ggaacactac ggcggactgc aaaaccgttt
     7321 tgccgaagcc gttgccctca acacaggcga actctccgcc cttttgaaaa acaaatcctt
     7381 cggcgagaaa aaagcccgca aaatcgaaca ggcggcaaaa atgcccgcct tctggctcga
     7441 taccgaacac accggccgac cgcccggaca cacaggaaaa cacaccatgt cccatatttc
     7501 ccccatcccc gaaatcctag ccgacatcaa agccggtaaa atggtcatca tcaccgatgc
     7561 cgaagaccgc gaaaacgaag gcgacctgct gatggcggcg caattcgtta cccccgaagc
     7621 cgtcaacttt atgatcaaac acgcgcgcgg cttagtctgc ctgcctatgg aaggtgcaat
     7681 ggtcgaaaaa ctcggtctgc cgatgatgac ccaaaaaaac ggcgcgcaat acggtaccaa
     7741 ctttaccgtt tccatcgaag ccgcgcacgg cattaccacc ggcatttccg ccgccgaccg
     7801 cgccctgact attcaaaccg ccgtttcccc gaccgccaaa cccgcagaca tcgtccaacc
     7861 cggtcatatc ttcccgcttc gcgcccaaaa aggcggcgta ctcgtccgtg ccggacacac
     7921 cgaagccggc gtcgacttgg cgcaaatgaa cgggctgatt cccgccgccg tcatctgcga
     7981 aatcatcaac gacgacggca cgatggcgcg tatgcccgaa ctgatgaaat tcgccgaaga
     8041 acacaagctc aaaatcggca cgattgccga cctcatcgaa taccgcagcc gcaccgaaag
     8101 cctgctcgaa gatatgggcg acgcgcccgt acaaactccg tggggcgagt tccaacaaca
     8161 cgtttacgtc gacaaactct ccggcgaaac ccacctcgcc ctcgtcaaag gcacaccctc
     8221 cgccgacacc gaaaccctcg tccgcgtcca cgaacccttc agcgtgatgg acttcatcca
     8281 agccaatccg cgccattcct ggtcgctgcc caaagccctt gatcgcgtcc aacaggccga
     8341 aagcggcgtc gtcatcctcc tgcaccacac cgaagacggc gcatccctgc tcgaccgcac
     8401 cctacccaaa ggcgcaaacc aagcctacaa atgggacagc aaaagctacg gcatcggcgc
     8461 gcaaatcctc gccggcctcc acgtcaaaaa actgcgcgtc ctcggccaac cgtcatcttt
     8521 caccggcctc accggcttcg gcctcgaagt cgtcggcttt gaagaagcgg aaaaataata
     8581 tagtaaattc aaatgcttta tatttgcttc atttattgca ttatttccgt acaaacgaaa
     8641 acccggtctg ttgggttgga ttttgctttt ttcggatttc gggtaacttc taattcgtca
     8701 ttcccgcgca ggcgggaatc cggttcgtcg ggtttttgtg tcatttccga tgaattcctg
     8761 tggctttggt tttttggatt ctctctttcg gggaaagaac ggcataagta ttttccaaac
     8821 caaacaaaat gccgtctgaa aggctttcag acggcatttt aagtttgacc ggtttcattc
     8881 atcggtattt atgaattgaa tttcaacatc gccaatctat ccttaatctc cttttccaat
     8941 tcggcagatt cggcgaaaag tttatccaaa tccgctgaaa atcccgctat tttttgcaca
     9001 aattcgtcgg cggatatatc cacataatca atttttacct caaaatactg ccccgccgac
     9061 aagctgtgat tcttcgcttt gatttcatca tagccgacca ccacgctgaa atcttctact
     9121 gcctgtttgt gcgtgaaggt atggcagatt ttttgttctt cttcgtggga aagtacagtt
     9181 ttttggttgt cattaatact aattttttca cctaaaccgg aagcatcgat taatacaact
     9241 ttatccttat tgactttatc aataaacaag atagaaacat tggttcccgt cgtagcgaaa
     9301 atattggaag gcatagacac cacgccggcg agcattttgt tttccaccag atattcgcga
     9361 atttttttat cgatgcccga ttttgccgtg ataaagccgg tcggtaatac aatcgctgcc
     9421 ttgccgtttt ctttcaggct gaacagaata tgctggataa aaagctgata gatttccatc
     9481 ttctctttct ttgttggctt gattttcggg ataccagcaa aaaagcgttc gtggttttca
     9541 tcgctttcca gccgatcgcg gaaatcgctg aaatccaatt taaacggcgg attggaaaca
     9601 atgaaatcaa attttttcag acagcctgaa gcgtctttgt gggcgggcga caaaatcgta
     9661 ttgccctgaa ccacattatt caaagaatgc accaaattat tcaaaatcag attcagccgc
     9721 agcagattgg acgatttctg cgaaatatcc tgcgtataaa tcatacattt gtcttcgcct
     9781 atggcatggg cgacgttcat caacagcgtt cccgaaccgg ccgacgggtc gtaaacgtcc
     9841 acgctgcgga tttgcccgcg cacatcttcc ggcaccaaaa tatccgccat aatccgcgcc
     9901 accgcatgcg gcgtgtagta ttcggcatat ttgccgccgg aattactgtt gtagtcttta
     9961 atcaggtatt caaaaatcgt ggcgaaaaag tcgaattttt gcgcaaaaat cgcctcgaag
    10021 ctgaaacccg ccagcttgga aatcagcgcg cggcaaaaat cgtcgcggcg accttcgtca
    10081 gtaatgtact gactaatacg ctcaaacaac acgattttcg ccccgccttc ggttttcaca
    10141 gaaaacaact cagcattatg tgcagcgatg tcagtgagcg tatcgtcaaa gagtttggca
    10201 aagtctgcgc cgttttgccg ctccgacaaa tacttgatgg aatgttcggg ctttaaaacc
    10261 gctgtttttc catcaatatc catattgacg aactcgatcg gctcgtcggg cttttctttg
    10321 cgaatttttt taacttcaaa atcgtatttg tcgtttagga atttatacaa aaacgcctga
    10381 ctgatgattt taaattcgtt gccgtcattg cccaaaccat agttggcgca gatggttttt
    10441 aagctgtcga ttagggattt gatttgttcg gtaaagtgtt gttcggtcat ggaatgtctc
    10501 ggtttgttgc tagcgtcgct taaattttgt ttagcattag ttggtttgct ggcagattaa
    10561 ataggttgaa cactatttgc caaccttttc tcttttccta atgctgttgt gtaataacgt
    10621 tcatcaatac catataacaa atgtttcagc tgtacatcag tagaaatttc tacacgagag
    10681 ccatctgcgt taaatttcaa gatattatta ttatattcag caaggtaatt taatagttct
    10741 tgtctttgat ctagcgtcat attattcaat ctatcttgaa ctaaagctat tcttttccta
    10801 ttgggaatag atactttttc acttaaaaaa tcttctttta aatcaataaa atcgctttct
    10861 aagaattgct gtacttcagt a
//
LOCUS       DAXFZR010000074        10825 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000074 DAXFZR010000000
VERSION     DAXFZR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.075"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            140..1249
                     /gene="proB"
                     /locus_tag="ACFJAS_001700"
     CDS             140..1249
                     /gene="proB"
                     /locus_tag="ACFJAS_001700"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC3982889.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            1262..2524
                     /locus_tag="ACFJAS_001701"
     CDS             1262..2524
                     /locus_tag="ACFJAS_001701"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC3982890.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEVAAEDI
                     LAANRLDLKAAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLER
                     LLVRGRIEAGLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEARGTHHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(2700..5744)
                     /locus_tag="ACFJAS_001702"
     CDS             complement(2700..5744)
                     /locus_tag="ACFJAS_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC3982891.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            5909..6589
                     /locus_tag="ACFJAS_001703"
     CDS             5909..6589
                     /locus_tag="ACFJAS_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982892.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTAALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSERGIWL"
     gene            6618..6977
                     /locus_tag="ACFJAS_001704"
     CDS             6618..6977
                     /locus_tag="ACFJAS_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFC3982893.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            7055..7161
                     /locus_tag="ACFJAS_001705"
                     /pseudo
     CDS             7055..7161
                     /locus_tag="ACFJAS_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7582..8118)
                     /locus_tag="ACFJAS_001706"
     CDS             complement(7582..8118)
                     /locus_tag="ACFJAS_001706"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC3982894.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(8152..8508)
                     /gene="folB"
                     /locus_tag="ACFJAS_001707"
     CDS             complement(8152..8508)
                     /gene="folB"
                     /locus_tag="ACFJAS_001707"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC3982895.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            8564..9166
                     /gene="plsY"
                     /locus_tag="ACFJAS_001708"
     CDS             8564..9166
                     /gene="plsY"
                     /locus_tag="ACFJAS_001708"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC3982896.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHPSWIFATLAIAILVLLRHKSNILNLIKGKEGKIGEKR"
     gene            9258..10103
                     /locus_tag="ACFJAS_001709"
     CDS             9258..10103
                     /locus_tag="ACFJAS_001709"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC3982897.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     VILIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            complement(10211..10543)
                     /locus_tag="ACFJAS_001710"
     CDS             complement(10211..10543)
                     /locus_tag="ACFJAS_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGK_0946 family protein"
                     /protein_id="HFC3982898.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(10593..10823)
                     /locus_tag="ACFJAS_001711"
     CDS             complement(10593..10823)
                     /locus_tag="ACFJAS_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982899.1"
                     /translation="MLLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQISERAER
                     AEYRKERREEMMDAARAIKKANENSPNIYFIR"
BASE COUNT         2479 a         3163 c         2667 g         2516 t
ORIGIN      
        1 ctataaattt tattaatttt atacattttg tctattttat tgcagatgcg gcgtgattgt
       61 tgacaattgc gacagaacag ggcaaattcg gcggtacaag gcggcaacga gacaggacaa
      121 accaacaagg acggaaacaa tgaaatacaa aagaatcgta tttaaagtcg gcacatcttc
      181 gattacccat tcggacggca gcctctcgcg cggcaaaatc caaaccatca cccgccagct
      241 tgccgcattg catcatgcgg gacacgagct ggtcttggtg tcttccggcg cggttgctgc
      301 agggttcggc gcgctgggtt tcaaaaaacg tccggtcaaa atcgccgaca aacaggcttc
      361 cgccgccgtc gggcaggggc tgctgatgga agaatatacg gcaaacctgt cttcagacgg
      421 catcgtgtcc gcacaaatcc tgctcagccg tgccgacttt gccgacaaac gccgctacca
      481 aaatgccggc ggcgcacttt ccgtgctgct gcaacgccgc gcgattccca tcatcaatga
      541 aaacgacacg gtttcggttg aggagttgaa aatcggcgac aacgacacat tgagtgcgca
      601 agtggcggcg atgatacagg cagacctctt ggtgctgctg accgacatag acggtcttta
      661 caccggcaac ccgaacagca atcccgatgc cgtacggctg gacaaaatcg aacacatcaa
      721 ccatgaaatc atcgaaatgg cgggcggctc gggttcggca aacggcacgg gcggtatgct
      781 gaccaaaatc aaagcggcaa ccatcgccgc cgaatccggc gtaccggtgt atatctgttc
      841 ctcactcaaa cccgattcat tggccgaagc cgccgaacat caggcggacg gctcgttttt
      901 cgtcccccgt gccaaaggtt tgcggacaca gaagcaatgg ctggcgttct attccgaaag
      961 ccggggcagc gtttatgtgg acgaaggtgc ggaacacgct ttgtccgaac aagggaaaag
     1021 cctgctgatg tcgggcattg ccggaatcga agggcatttt tcccgtatgg acaccgtaac
     1081 cgtgtacagc aaggcaacca aacagcccct gggcaaaggg cgcgtccttt tcggctccgc
     1141 cgccgccgaa gacctgctca aatcgcgtaa ggcgaaaggc gtgttcatcc atcgggacga
     1201 ttggatttcc atcacacccg aaatacgcct gcttctgacc gaattttaga aaaggaaacc
     1261 catgtcaaac acacaaaaac agcttgccct tgccaaagcg gcaaaaaaat ccgtcaacac
     1321 ggcggatacg gaagaaaaaa accgcgcgct gcttgctatg gcggacagtc tggaagtggc
     1381 ggcggaagat attttggcgg caaaccgttt ggatttgaaa gcggcggcag gcaaaattcc
     1441 cgacagcatg accgaccgcc ttttgttgga cggcaaacgc atttgcgcga tggcggacgg
     1501 catcagggcg gttgccgcgc tgcccgaccc cgtgggcgaa atactggaaa cctcgactct
     1561 gcccaacggc ttggaaatcg tcaaaaagcg cgtggcgatg ggcgttatcg gcattattta
     1621 cgaaagccgc ccgaacgtta cttccgatgc ggcggctttg gcactgaaaa gcggcagcgc
     1681 ggtcgtactc cgcagcggca aagatgcatt tcaatccgca cgcgccatcg ttgccgccct
     1741 gaaaacgggg ttggcacaaa cccgcatcga ccccgaagcc gtacagctca tcgaagacac
     1801 agggcgtgag ggcagttacg aaatgatgag ggcgaaagat tatctagacc tgctgattcc
     1861 gcgcggcggg gcagggctga tacgggcggt ggttgaaaat gccgtcgtgc cggtcattga
     1921 aacggggacg ggcatcgtcc acatttatat cgacaaggac gcggattggg acaaggcact
     1981 ccgtatcgtg tacaacgcca aaaccggccg cccgtccgtg tgcaactcga tggaagtgct
     2041 gctggtgcat gaaggcattg ctgccgactt cctgcccaag ctcgaacggc tgttggttcg
     2101 cggccgtata gaagccggac tgccgcccgt ccgcttccgt ttggatccgc aggcggcgcg
     2161 gtatatcggc ggcgaagcgg cgggtgcaga cgatttcgat accgagtttt tagactacat
     2221 cctcgccgtg aaaaccgtcg cttcggttga agaggcagtc gggcacatcg aagcacgcgg
     2281 cacgcaccat tcagacggca tcgttaccga aaaccgccac gctgcggact atttcacgac
     2341 ccatatcgat tctgccgccg tgtatatcaa cgcgtccacg cgcttcaccg acggcggcga
     2401 attcggcttg ggttgcgaaa tgggcatctc cacgcaaaaa ctccacgccc ggggcccgat
     2461 gggtttgaaa gagctgacga gttacaaata catcgtacaa ggcacgggac aggttaggga
     2521 ataatccgaa ttcaaacgtc tgatatagtg gattaaattt aaaccggtac ggcgttgcct
     2581 cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
     2641 tccactataa aaatgccgtc tgaaacagtc ttcagacggc atttctattt gcaaaaaaat
     2701 caagcattgt ccaaggggac gagaatcgta cggttgccgt tgtgttccgg tgcggacaca
     2761 atgccttccg cttccatttg gtcaatcaga cgcgcggcgc ggttgtagcc gatgcgcaag
     2821 gcgcgctgta cgcccgaaat gctggctttg cgcgttttca ggacaacgga tacggcctcg
     2881 tcgtacatcg gatcggtttc gccgtcgccg ctgcgcccga tgccgggcag ctcttcgctg
     2941 ccgccgccgc tcaaaatatc gtcaacatag tccggctcgc caaactgctt cagatattcg
     3001 accacgcggt gcacctcttc atccgaggca aacgcgccgt gaacgcgctg cggataggca
     3061 gtacccggcg gcaggaacag catatcgccc tgaccgagca ggttttccgc gcccatttgg
     3121 tcgagaatcg tgcggctgtc gattttgctg gacacttgga acgcgatacg cgtcgggatg
     3181 ttcgccttaa tcagacccgt gatgacatcg acgctggggc gttgtgtggc aaggataagg
     3241 tggatgcctg ccgcgcgggc tttttgggcg aggcgcgcaa tcagttcttc gattttcttg
     3301 cctgccgtca tcatcaaatc ggcaaactca tcgaccacga ccacgataaa cggcagtttt
     3361 tccaaaggtt cgggatcgtc gggcgtgagg ctgaacggat tgccgatttt ttctcccctt
     3421 gctgcggctt cggcgatttt ttggttgaag cccgcaagat tgcgcacgcc cataaagctc
     3481 atcaggcggt agcgtttttc catttcgtta acacaccagt tcagcgcgtt tgccgccagc
     3541 ttcatatcgg taacgacagg ggcgagcagg tgcgtgatgc cttcgtaaat gctcaattcc
     3601 agcattttcg gatcgatcat aatcatacgc acgtcttccg gcgcggcttt gaaaagcatg
     3661 gacagaatca tcgcgttgac acccaccgat ttgcccgaac cggtcgtgcc ggcaaccagc
     3721 aaatgcggtg ctttgcccaa gtcggttacg acgggctgtc cggtgatgtc ctgaccgagc
     3781 gcgagcgtca gcttggattt ggattcggca aactcgggcg aattgaaaat ttcgctcagg
     3841 cgtatcattt ggcgtttcgg gttcggaagt tccaaaccca tgcaggtttt gccggggatg
     3901 gtttcgacaa cgcggatgga agccacgccg agcgaacgcg ccaagtcttt ttccaaattc
     3961 agaacggaat tgccgcgcac gccgacatcg ggttcgattt cataacgcgt aatcacgggg
     4021 ccggaataag aatcgacaac cttgaccttg actttgaact ccgccaattt ttcttcgatg
     4081 gtgatgctgt tttccaacag ttcttcttcg gtttgcgtcg cctcgggatt gaacagcgga
     4141 ggcagaagca ggtcggttgt cggcaggtgt tcggataccg caccggtctc ttccgattgg
     4201 aacgcccctt catccgcttc cgtatccgat acccggcagg acgggcgttc agacggcaca
     4261 tcttcaaacg gacaaaccgc ctgactgtca tgcccgaacg cttcggtttc cgacagatat
     4321 tgcccgcttg accgctctgc cgcaccctca ctgccgtcat ctgcaatagc ggcggtttcc
     4381 tcctgccaac ctccattcaa aacatcagcg gcaagatggt cggtttcggc aatgcggctg
     4441 cgttgcgcct gctcgaatcc tgccggcggc tcataggtac ggttgtagat ttccgatacg
     4501 ggaggcggcg gcagaatatc gatttcgggt acggctacct ccggcacttc gggtggttcg
     4561 accacccaag catcgggcgc ggcaggctct tcaatatgga tatcggcagc ttcatcataa
     4621 accggaccgc cgtatcccac agacgaaacg gtttccgtga aaacattttc ggtttcagac
     4681 ggcatttcga catcccgttt cccgacaacc gtccgacttt ccggaatcag gcgcgaaatc
     4741 cgcgcctccg cagaaaccgt tgccttgttc ggttctttca aattgacgga aacgcggcgg
     4801 cgggcatcgc ggacggctgt gccgtctgaa acggactgcc cttgggaaac atccggggat
     4861 tttgcctccg cctgcccttt ctcctgccct gcatgacggc ggaacggcgc attccgtgca
     4921 ttttcggctt tgactgcaga aaaggcagac ggatgctgct tctcaaaata cggctcaaat
     4981 ccgtaatccg cagactcgga aaacgcttct ttgtccgcat caaacatacg ggaatacgtg
     5041 cgttgaagca cagggtcgtc caaaccgata atctgcagcc cttccatcgg ggtatcggac
     5101 acgcgtactt tggggacggc tgttccgttt ttctcaaatg catcgatata gcgttttttc
     5161 gtttccctta aagccgcgct gcttaaagca cgcgttgctt cttccaaagt gatttcctta
     5221 aaaaccggac ggacgggcga agttttgctt tcagactgca tcaacccttc agcaatactc
     5281 cggtcgaatg ggatgcggcg gttgtcgatt acggcagttg caatgtcttc cgtatcggca
     5341 gcctcttcct ccgcagcttc tgctgcttcc gtttccgctt cttccgtccc gtttccactg
     5401 tctgaatacc cgtcggatgc ggtcggaaca tccgcacggt ttgcagaagc aggcggaacg
     5461 ggcctgtccg gttcggattc gtgcgccaga tagtttcggc agaaacggac gacaccgaca
     5521 aacagccaat acactgccgt tttgacggca tggaaaagca tcagggaaaa ctcgggaaaa
     5581 tcgggcatac cgtctttgat ttcaggcagc tccgcctgtt tttccccttt cttttcctgc
     5641 cacgcagaaa cctcgcgcat ccactcgcgt tcggattgtg cgcggacaaa aaacaggccg
     5701 gcaagcgcaa gcaacaaaat aacgatcaaa actatccaaa acatatgccg tttcccgcaa
     5761 gacaaacgat taaccgagtt tattttaacg tgtttccagc ccaaaaaaaa caaccggccg
     5821 gcaattctgc cgaaaccgat tcggctttcg gtttgccccc gccgacagta taatagccgc
     5881 cccttccctt aatctttgcg gcattaccat gattttccaa acagtctggt tttcagatgt
     5941 ggtactgtcg gtttcgtgga ttgtccttat cctgatactg gcagcttccg cgccgtcggc
     6001 attccgttcg cttgcccgct accgttccgc ccttcccctg tgtaccgtaa ttttttccgc
     6061 agcatggtgt ctgaacgcct ctgtcggcgg cggacagctt gcccagatga actaccacct
     6121 gcttgccgtc aatctggtta cactgatggt ggatacctcc gccgcccttt ggcttgccgc
     6181 gctgctgatg ctgccctact gcctgctgtt tgccggttct gccggcgcat atccgccgaa
     6241 cgctttggtg ctgatactgc ccgcgcttgt cgtcaaccgc ctgtcgcgta tgctggtcaa
     6301 ccgtctgccg cccaatattt tcatctttat tttcgtcaac ggctttctcg cttccgccgc
     6361 cggcattctg ctgaccgggc tggtgctgat cggtattttg gatgccgcaa acgcttttcc
     6421 gtccgaaata ttgtggacgg ccgccctgcc cgtctttatt ctgctggcgt gggcggaagc
     6481 cttcctcagc ggcatttcaa ctgccatttt cgtggcactg aagccccatt ggatcaacac
     6541 tttcgacgac aaccgctatc tgaagtccga gcgcggcata tggctgtagc cttcagacgg
     6601 cataggcaga caaaaccatg ctttccaaca tcctccccct ttccatcggt gcaatattcg
     6661 gcacaacggc gcgttggctg ctcaacctcg ccgtccccgc atcgttgtct cccgccacag
     6721 gcaacctgtt tgccaactgg acgggcgcgc tgctcatcgg catctttgcc gaaaccgtca
     6781 gccatccgca atggaagctg ctgctgatta ccggtttttt cggcagcctg accacgcttt
     6841 ccggattttc actggaaacc gtaaccctgc tccaatcgaa ccgtcccgct tcggcacttg
     6901 ccaatatttt cctacacacg gcaggttcgc tgctgctgac ttggctcggg ctgaagatag
     6961 ggactgcagt caaataaccg caaaaaattg cttcctttgg ttttgacata atctataaat
     7021 tggcttcgct tgtttaaata ctgcctgtgc caattatagt ggattaaatt taaatcagga
     7081 caaggcggcg agccgcagac agtacagata gtacggcaag gcgaggcaac gccgtaccgg
     7141 tttaaattta atccactata ccgttctcaa attcaaattg ggcttttagg tctataacaa
     7201 agaaaaagcc ttgggaggat aaacctacca aggctttttc ttaaaaaacg ctctatcact
     7261 gtgcctaaaa atgcaaaaaa gtaaccgtca taatcctttt gccaaaatca atatcatcat
     7321 ccgatgaatg aacggacggt atataagaaa gcgttaataa actcataaac cagtaagaaa
     7381 aatcagtaac gaagatattc attattaccc attcttaaaa attatcacgt tcgtttgggt
     7441 aattatcata cccttttccc tcttattact aaagcacgaa ttactttaaa gtacgcaccc
     7501 agagacaagc cgaaaaggca tatccgatcc gccaaacaaa atgcccgccg acttactccg
     7561 gcgggcggtt ccaacatcct gtcaatcctt catcagccag tattgcaaac cgattaatgt
     7621 tttaccgtct ttaatttcat cgtttgccaa tgcctgacgg acttcatctt tcgacatcag
     7681 cacggtttcc gttatttcat cttcatcatt agaaagcgta ctgcccaagc gcacgccttc
     7741 cgcctcgaac agatacattt tttcattgca aaaaccgacc gccgtataaa aactgtacag
     7801 caggcgcacg ctgtcggcaa cataaggcgt ttcctccgcc aattctcgca gcgcacacgc
     7861 tgccatatcc tcgcccgcca catccagctt gcccgcagga agttccaatg tcgcctgatt
     7921 tgccgcataa cgccactgcc gcaccaaaac gaccttttcg tcatccgtta ccgccaacac
     7981 gcacgccgcg cccggatgcc ggatgacgat gcgctgccct tcattgccgt tgggcagcct
     8041 gaccttatcc ctgctgatac tgacgaaacc gccctcgtaa atggtttcgc cgcctaattt
     8101 tacttccctc aaatccactt catatctcct ttccgtttcc tattctgccg ttcaatcttc
     8161 acgcttgccg cgctcgattt ccactccaac ctcgcgcacg ccttccaaaa tacccggctt
     8221 gacgattttc acgtgcaccc acaccgcgcc gaaatatccc aaaaccaaat cggcaatata
     8281 ttccgccaac gcttccaaaa gcaggaaatc ctgttctttc agatgtcggc gcagcgtttc
     8341 gcatacctcg gcataatgca ccgtattggc aatatcgtcg tccgaacccg ctttctcggg
     8401 aacgccgatg tccaaatcga caatcagggt ctgcaaccgt tcgcgttccc agccgtacac
     8461 gccgataagc gtatccgcct tcatgccgtg caaaaagatt ttatccataa tccgccccgt
     8521 tttttgctaa aataaccgca ccattctaag taaagtaccg caaatgttca acataccggc
     8581 cgtcgccgtt tcctatttaa tcggttcact ttctttcgcc gtcatcgttt ccaagtatta
     8641 cgggatggac gacccgcgca cctacggatc gggcaatccc ggcgcgacca atgttttacg
     8701 cagcggcaaa aaaaaggcgg ccgcgctgac gctcttgggc gatgccgcca aaggtttggt
     8761 tgccgttttg cttgcacgcg tgcttcaaga accgctcggt ttatccgaca gcgcaatcgc
     8821 cgccgtcgca ctcgccgcgc tggtcgggca tatgtggccg gtgtttttcg gatttaaggg
     8881 cggcaaaggc gtggcaacgg cattgggcgt gcttctggca ctctctcctg caactgcctt
     8941 ggtctgcgcg ttgatttggc ttgtgatggc attcggcttc aaagtatcct cccttgccgc
     9001 gctggtcgcc acaaccgccg ccccccttgc cgcactgttt tttatgcccc acccttcttg
     9061 gattttcgca accctcgcaa tcgccatatt ggtgttgctc cgccataaga gcaacatcct
     9121 caacctgatt aaaggcaaag aaggcaaaat cggcgaaaaa cgctgattcg gcatttgttt
     9181 tccattacgt catgccgtct gaaaccatgt tcccgcgttc agacggcatt tccgcacaaa
     9241 cggctttcat acataccatg ctcagttaca gacacgcatt ccacgccggc aaccacgccg
     9301 atatgctcaa acacttcacc ttgtttttag tgctgcaata tttcaaccgc aaagacaagc
     9361 cctactggta catcgacacg cacggcggcg cgggtttgta caatctcgaa ggcagcgagg
     9421 cgcagaaagt cggcgaatac cggcaaggca tcgccctgct ccgacaggcg caaaacctgc
     9481 ctgccgaact ctctgacttt gccgcgcaca tacaaaaaat cctaccctcg cccgaacttt
     9541 actgcggctc tccgtggctg gcgcaatcgc tgatccgcat cggcgacaaa ttgcgcctgt
     9601 ttgagctgca ccccaccgac tttgtccatc tgcaaaacaa tatgggcgaa gcgggattgg
     9661 gcaaacgcgg acaagtgttg cgcgaagacg gttacaaagg gctgatttcc ctgctgccgc
     9721 ccccgccgcg acgcgctgtt attctcatcg acccgcccta tgaggaaaaa caggactacc
     9781 ggcgtgtaac ggaaacgctg aaagccgctt taaaacggtt tgagtccggc tgttatctca
     9841 tctggtatcc ctgcctcagc cgcgaagaaa gccgcaagct gccggaagaa ttgaaaaaac
     9901 tcgttcccga caactacctt cacgccgaac tgcacgtcca cgcgccgaaa accgacggct
     9961 tcggtatgca cggcagcgga atgttcgtca tcaatccgcc ctatttgttg gcggaacaat
    10021 tggcggcaaa cctccccgcc ctgacacggc tgcttgcaca agacgaaggc gcgcgctacc
    10081 ttttggacag caaaatccgc tgaccccgat gccgtctgaa cccttcagac ggcatcttcc
    10141 gtcaaacaaa caaaaccggg cgatacacac tccatatcgt ccggttctgc ataaaaccag
    10201 tattcaaaaa tcagaacacg cttttcatta cacccacggt accaaccaca ccgttcagca
    10261 gcatcagacc accggcagcc gtacccatac cttgacggat tgcgtcatgc ttattggcac
    10321 gtttttcacg ttccaaagcc aactcctcag aaacattttt acgttgacgg cggtgttgcg
    10381 ccagttccgc acggctaatt cgcgcttcgg cacggttacg accagccttg attacggccg
    10441 aatcgtcgcc gatagcatag ttggctacag aattttcaga agcgcaggca gcaagcaaaa
    10501 cggcacacac agcaacaacg gaaatatttt tcaagttttt catacctgtc ctattatctc
    10561 aaaatgaaga aaaaactaat ccaactggag ttttatcgga taaaataaat attgggtgaa
    10621 ttttcgtttg cctttttgat ggcgcgtgcc gcatccatca tttcttcccg gcgttcttta
    10681 cggtattcgg cacgctcggc acgctcgctt atctgaatgg cacgactttt cgcctgtatt
    10741 tccgctgcag attcttgagt tacaaaacgg cggtctgcgt aagtacatgc cgtcaccaac
    10801 atcgcagcca ccgacaacaa aatca
//
LOCUS       DAXFZR010000075        10412 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000075 DAXFZR010000000
VERSION     DAXFZR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.076"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(483..1394)
                     /gene="crgA"
                     /locus_tag="ACFJAS_001712"
     CDS             complement(483..1394)
                     /gene="crgA"
                     /locus_tag="ACFJAS_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC3982900.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            1633..2211
                     /locus_tag="ACFJAS_001713"
     CDS             1633..2211
                     /locus_tag="ACFJAS_001713"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC3982901.1"
                     /translation="MNILLLDGGKAFGHSHGGLNHTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVFTGGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFIGLS
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(2419..3804)
                     /gene="pntB"
                     /locus_tag="ACFJAS_001714"
     CDS             complement(2419..3804)
                     /gene="pntB"
                     /locus_tag="ACFJAS_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC3982902.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLEGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(3884..4267)
                     /locus_tag="ACFJAS_001715"
     CDS             complement(3884..4267)
                     /locus_tag="ACFJAS_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC3982903.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(4271..5809)
                     /locus_tag="ACFJAS_001716"
     CDS             complement(4271..5809)
                     /locus_tag="ACFJAS_001716"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC3982904.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            6098..6931
                     /gene="serB"
                     /locus_tag="ACFJAS_001717"
     CDS             6098..6931
                     /gene="serB"
                     /locus_tag="ACFJAS_001717"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC3982905.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFEQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            6995..8217
                     /locus_tag="ACFJAS_001718"
                     /pseudo
     CDS             6995..8217
                     /locus_tag="ACFJAS_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            8378..9958
                     /gene="purH"
                     /locus_tag="ACFJAS_001719"
     CDS             8378..9958
                     /gene="purH"
                     /locus_tag="ACFJAS_001719"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC3982906.1"
                     /translation="MSVIKRALISLCDKAGAVEFAQTLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     AGNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDGVLSGQPQIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYCDIDPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGIAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDINR
                     INRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         2407 a         2736 c         2806 g         2463 t
ORIGIN      
        1 taagtatttt cttagagggt tttgttttac cggcggtcag aaacgcattt ttgagatgat
       61 tgttttgaga tggaataaaa tctttgcaaa attcctttcg tgatggttat gaaaaataaa
      121 tttctaaaga tttaattggt tatttttata attttgaaat ttatcggttt tgtaaaggtc
      181 tcgggataag caatttcggt cttcggcgcg tcttttacgc caaacccata agaattttga
      241 gggtaatctt catcgttttg cctgaattgc cgacgcaaag cgatttatcg gcggttgtgt
      301 ttgagcgttt gattgcagct tgcccaatct caaatatttc taaataccaa ttgaaattca
      361 atattttata aaaacactga tttggaacga acagatttat aaaaattccg tgtatccttg
      421 agtaagggag ttgattgaaa agacttgttt gattgatgat gaagaataag ttaattaatg
      481 tcttatttgg tattcgttct attcatattt tttcccagtt ccttcactaa aaaatccaaa
      541 aatacgcgta agcggaggtt gacggcttta tcgctgtaat aaacagcatt aaaggggtgt
      601 gttttattgg aggtttgttc ggcgaatagg ggaattaact ttccttcagt gatgtcgttg
      661 tcaaccaaaa aatctgataa gcaagcaata ccgcaacttg aaaggcacaa cgagcgtaag
      721 atttcaccgc tgctggcggt aaagtgcggt gaaattttat agggatttcc ctgcgcatct
      781 aaaaccgccc atgtatttag agaaccgggt tctgtgaagc ctaaacattg atggttggca
      841 agatcttctg cagattgtgg cgtgccgtgt tttgctaaat attcaggact ggcgactacg
      901 cggaagtggc tgtcaaacag atggcgtgca cgcagcccgg aatcgtccaa ttctccggcc
      961 cgtaaggcaa tatcgacttt gcgttcaatc agattgatat agccttcgga agaaacgagc
     1021 gaaagtcgga tatgcggata gcgttcgttg aattttgctg ccagcggcgc cagcagatgc
     1081 agcaccatcg gcatcgcgga atccacgcgc aacacgcctt gcggtacttc gtgcactgcc
     1141 agcatttcgg tttccgccgc tgccatttct tgcaggattc tctgcgcgcg gcggaaatat
     1201 tgcgcgcctt cttccgtcag attgagttgc cgcgtggtgc ggttgagcag gttcacgccc
     1261 aacttttcct ccagccgttt gacgatgcgg cttacggcag aatttgccat ctccaactgc
     1321 tccgccgcac ggctgaagct gccgctttcc accacttgaa caaatacggt cagttcttct
     1381 gaattggttt tcatcgtgtt tccttttcgg ttggaacccc accctttagg gcggcaggat
     1441 cagactttat ttgggaaggg cgtaacccct tcccaatcag gacggcacat agggagacga
     1501 tttatgtgtc atcccgtgtg ttgaaacatc tgattattcc atttgacgca aaagtgtttt
     1561 cttatttttg cacttttaaa tcataaagta aaacggtaca atacattcat caattcacaa
     1621 acgaggtaac aaatgaatat tttattatta gacggcggca aagcgttcgg acattctcac
     1681 ggcgggttaa accacacgct tcacaaaaaa gcgaaagaag ttttgaccgc gctcggacac
     1741 aatgttcaag aaaccgtgat tgatgccggc tatgatgttg aggcggaaat cgaaaaattc
     1801 gtttggatgg atgctgtgat ttggcagatg ccgggctggt ggatgcacga gccttggaca
     1861 gtgaaaaaat atatggacga agtctttacc ggcggacacg gcaaactcta ccaaagcgac
     1921 ggcagacacc gcgtcaatcc gactgagggc tacggcacag gcggcttgtt gcaaggcaaa
     1981 aaacatatgc tttcactgac ttggaatgcg ccgattgaag cctttacccg cgaaggcgat
     2041 ttctttgaag gcaaaggcgt tgatgttttg tatatgcact tccacaaagc caacgagttc
     2101 atcggcttga gccgcctgcc gacattctta tgtaacgatg tggttaaaaa tccgcaagtg
     2161 gaaaaatact tggcagatta tcaggcacac ttggaaaaag tgttcggcta attaaaaatc
     2221 catcttcaac acggagatgg attttgtttg tttcgttgat tttgtgtcag tttcagatgt
     2281 gggtgcttat ccggacgggt cgtctgaaaa tgtttgcccc aatgcaaaaa aatcactgca
     2341 aaccttcaca aacggggctg gtagtgattt tttcaaatca aacagattga aaacctgcgc
     2401 cgaattgttc agacggcatt attttttcaa ttccgccaaa atcccgtcaa cggttttctt
     2461 cgcgtcgccg aaacacatca cgctgttttc gttgaagaac agcgggtttt gtacgcctgc
     2521 gtagccggtg ttcatcgagc gtttgaatac gacgacttct tttgccttcc atacttctaa
     2581 aaccggcata cctgcaatcg ggctgttcgg atcggtttgg gcggcagggt tgacggtatc
     2641 gttcgcacca atcaccaaga ccacatcggt ttcggggaag tcgtcgttga tttcgtccat
     2701 ttccaaaaca atgtcgtagg ggactttggc ttcggcgagc agtacattca tatgaccggg
     2761 caggcggccg gcgacggggt ggatgccgaa gcgtacttcg atgccgtttt tacgcaaaag
     2821 ctcggtgatt tcggcaacgg ggtattgtgc ctgcgccact gccatgccgt agcccggggt
     2881 gataatgacg ctgcttgcgc cttccagcat ttcggcaaca tcggcagctt tgacttctcg
     2941 gtattcctct atctcttggc tgccggaaga cgatatgccg ctgtcgctgc cgaaaccgcc
     3001 ggcaatcacg gaaacaaacg accgattcat ggctttgcac ataatgtagg acagaattgc
     3061 gccgcttgag ccgaccagcg cgccggtaac gatgagcagg tcgttggaga gcataaagcc
     3121 tgccgctgcg gccgcccagc cggagtagga gttgagcata gatacgacca cgggcatatc
     3181 cgcgccgccg atggaggcaa ccaagtgcca gccgaatgcg agggcgatca gggtcataat
     3241 cagaaggatg aagccgctgc cgtcaatgcc gacaaatacg agcagcaaca caaacgatac
     3301 ggcgagcgcc agtgcgttga gcttgtgttt ggcgggcagt tgcagcgggc tgctgctgat
     3361 tttgccgttg agtttgccga atgcggccag tgagccggta aaggttaccg cgccgatgaa
     3421 aatgcccaaa tagacttcga ccagatggat ggtgtgcata tcgtgcgaaa cgttgcccgg
     3481 ctcgatgtag ctgttgaagc cgaccaaaac ggctgccaag ccgacgaagc tgtgcagaag
     3541 tgcaatcagt tcgggcattt cggtcatttc tacttttttg gctttgtgga tgccgattgc
     3601 cgcgccgatg agcatggcga tgataatcca gcccagtccg tgggtattgt cggaaaagac
     3661 cgttacgaag agggcgaccg ccataccggc gataccggaa tagcagcccc gtttggcggt
     3721 ttcctgtttg gacagccccg ccagtgagaa gatgaataaa attgcggcaa cgatatacgc
     3781 cgctgttacg agtcctgaag acatggaaat tctccgattt tcgatgattt gttttcaatg
     3841 ccgtctgaaa aattgacgtt cgtgttttca gacggcatct gtttcaagca gccgcgacaa
     3901 acagtccgac ggtgcaggca aatccgcccg cccacatcag cgagcgcaat gctgctttgt
     3961 ctgcgatgta gcaccagata aaggcgaggc ggaacaggat gaacaatccg gcaagcgtgt
     4021 tgacggttgc ttgtccggca ttgccggttg cgtgtgccgt caaaacggcg gcggcaaacg
     4081 gtgcaaaggc ttcaaaaccg ttttgctgcg cggcgtgggc acgggcggct gcgccttgcg
     4141 tatgtgccag aaaaccgcga ggattgtggt tgtctttaaa ccggaatccg cccgcttttt
     4201 tggcatacgc cgcacaaaaa agcggcaata ggcaggcaat cagaatgcac caataggcga
     4261 aagtcatggc ttaccctttc ttaaacatat tcagcatacg ccgtgtaacc gcaaagccgc
     4321 cgaagatatt gatgccggca atcaggatgg caacaaacga cagcagcgaa acgaagccgt
     4381 tgccctgacc gatttgcagc agcgcgccga cgacgatgat gccggagatg gcgttggtta
     4441 ccgacatcag cggtgtgtgc agcgagtggc tgacgttcca aacgacatgg tagccgatga
     4501 cgcaggcgag gacgaagacg ataaagtggt tcaagaatgc tgcgggtgcg accgcgccga
     4561 cccacagcac caatacggcg gcgatggcgg cgggcgcgag ttttttccac aggggaacag
     4621 gtttcggctc gggcttggcg gcaggcgcgg ctttttcaga cggcgtttgc tgcggctggg
     4681 cggaaacctg aatcggcgga ggcgggaagg tgatttcgcc gtcgcgggta acggtcatat
     4741 tgcggataat cacgtcttcg aagtccagcg tgatttcgcc gtctttgttc gggcttaaca
     4801 gcttggtcag gttcaccaag ttggtggcgt aaagctggga agactgtccg gcaaggcggt
     4861 ttgccatgtc ggtgtagccg atgattttca cgccgttgcc ggttacggac aattcgcccg
     4921 gtcgggtgag ttcgcagttg ccgcccgtcg ccgccaaatc gacgatgacg gatccggatt
     4981 tcatgctttc caccatttct ttggtaatca gcttgggagc gggtttgccc ggaatggcgg
     5041 cggtggtgat gatgatgtcc acttctttcg cctgttcggc aaagagcttc atttcggcgg
     5101 cgataaattc gtcgctcatc actttggcgt agccgtctcc gctgccgccc gattcttgca
     5161 ggaagtcgag tttcaggaac ttaccgccca tcgattcgat ttgttccgcc acttccaagc
     5221 gggtatcgaa cgcgcgcacc actgcgccga gcgaatttgc cgtaccgatt gccgccaaac
     5281 ccgccacacc tgcaccaatc accaaaacct gcgcaggcgg cactttgccg gcggcagtga
     5341 tttgaccggt aaagaaacgg ccgaaggcgt tggcggcttc aatcacggcg cggtagccgc
     5401 tgatgtttgc cattgaagac aaagcgtcca aggcctgagc gcgggaaatg cggggaacca
     5461 tgtccatcgc cagcgcgttg actttcttgg cgcgcaaggc ctcgaccaaa gcctcgtttt
     5521 ggcgcggcca caggaagctg acgatggttt gaccttcttt gagcagcggc agctcgcctt
     5581 cggacggcgc gttgacctta taaattaaag ggcaggccca aaccgccgct ttgtcggcaa
     5641 cggttgcgcc tgctgtttgg taagcggcat cgtccaaact tgccgccaaa cctgcaccgc
     5701 tttcgacaac ggtttcaaag cctagtttgc ccagcagggc aacggtggcg ggcgtgcagg
     5761 cgacgcgggt ttcgccggat aatgactcgc gtgggatacc gattttcatc tctgaatcct
     5821 ttttcgggtt gttcatatgt atcgtgggtt aaatttaaat cggtgcaggg cggagcaacg
     5881 ccgtaccggt ttaaaaccga ttcacttcaa atgttaatat attttagata atccccttat
     5941 aacgaatttt catcaggctg gcaatagttg cgcattttcc cgtgttgtcc gacacatact
     6001 gccgtttgcg tttgaagtcg ttttccgatt gcaggcacgc ccgccgtaac attctttgcc
     6061 gtttcactat ataatccgca ttttttgagc cgccgttatg ccgcaagccc tcgtcctcca
     6121 atttccctcc gccgaagccc tgccttccga cttcccctca cgcctgcccg aacctgatta
     6181 tgctgatgaa aagcgtatgc gttttattgt tgaagaagga ttttctttaa gcgaaaaaga
     6241 cgcggcgtta ctggacagcc gtcaaatcga ccacgccgtt ttgcccaata tggcgtttgg
     6301 cgaactcggg ctgattgtca gcgatatgga ttcgacgctg attaccatcg aatgtatcga
     6361 tgaaattgcg gcaggtgtgg gtttgaaagg caaagtagcg gaaattaccg agcgcgccat
     6421 gcgcggagag ttggatttcg aacagtcttt acgcagccgc gttgcgttgt tggcgggatt
     6481 ggacgaacag attttggcgg acatttatga aaacgttttg aagctctcgc ccggtgcgga
     6541 atttttgttg gacgaatgca aaaggcacaa tgtgaaattc ctgctggtgt ccggcgggtt
     6601 cacgtttttt accgaaaggc tgcaacaacg gctcggcttc gaataccaac acgccaatat
     6661 tttggaaatt gaaaacggca ggttgaccgg ccgtctgaaa ggcagaatca tcgacgcgca
     6721 ggcaaaggcg gatttattgc gcgaataccg cagccgcctc ggattgcagc cgcatcaggt
     6781 gttggcgatg ggcgacggtg cgaacgatat tccgatactc aaagaagcgg gcataggcgt
     6841 ggcttaccgc gccaaaccga aagcgcgggc cgccgccgat gcctgtatca acttcggcgg
     6901 tttggagcgt gtacgcggcc tgttcggata ggcggatagg aaacggatgc cgtctgaaag
     6961 gttttcagac ggcatttgaa cggcaggaac gacagtggga cgcagaaaac tttggtttgc
     7021 cctggcggca gcaggcgtta tcggcggttt ggtcggcatt gtgctgacgg aactgatgca
     7081 cttcatacgg catacggcat acggttatga cgcggacggt gcgtacatct cgttccgcga
     7141 aggtgtggca caggctttcg gtatgcggcg cgttgccatg ctgacgctgt gcggcgcgat
     7201 cgcaggcggc ggctggtggc tgctcaaacg tttcggcaag ccgcaaatcg aaatcaaagc
     7261 cgccttgaag cagccgttgc aggggctgcc gtttctgacg acggttttcc atgttctgct
     7321 gcaaatcata acggtcggac tcggttcgcc gctcggacgc gaagtcgccc cgcgtgaaat
     7381 gactgccgcg tttgcttttg ccggcggcag gcggttgggt ttggacgaag gcgaaatgcg
     7441 cctcttgatt gcctgtgcgt ccggagcggg tttggcggcg gtgtataacg tgccgctcgc
     7501 ctccacgctg tttatcctcg aagcgatgtt gggcgtgtgg acgcagcagg cggttgccgc
     7561 cgcattgctg acttccgtca tcgccaccgc cgtcgcgcgc atcggcttag gcgacgtgca
     7621 gcagtatcat cccgccaacc ttgccgtaaa tacttcattg ctttggtttt ccgccgtcat
     7681 cggcccgata cttggcgcaa ccgccgtctt tttccggcgc accgcccaaa aattcccctt
     7741 catcaagcgc gacaatatca aaatcattcc cttggccgtc tgtatgtttg tactcatcgg
     7801 tgtgattgcc gtttggtttc ccgaaatttt gggcaacggc aaagcaggca accaactgac
     7861 ttttggcggt ttgaccggct ggcaatacag ccttgagttg accgctgtca aatgggcggt
     7921 cgttttgatg gcgctggcgg caggcgcata cggcggactg attacgccgt ctatgatgct
     7981 cggcagcacc atttcttttg cagccgccgc cgcgtggaac agcgtcttcc ccgaaatgcc
     8041 gtctgaaagc gcggcagtcg tcggagcggc ggtgtttctc ggcgtatcgc tcaaaatgcc
     8101 gctgaccgcc attgtgttcg tattggaact gacctacgcg ccgctgtccc tgctgctgcc
     8161 tttgtgcatc ggcatggcgg ggcattggga acatcgggaa aaatgggctt caaatgaatg
     8221 ccgacaaatg ccgtctgaaa ggttttcaga cggcatttct ttgcaaattg tcaacaatat
     8281 ctgcaaaaac ctactgccaa aaaactgaaa atagcggata ataccgcccc gaaaaagatc
     8341 ccctttcacc atccaaccgt cagaaaagga agccccaatg tccgtcatca aacgcgccct
     8401 gatcagcctg tgcgacaagg caggcgcggt cgaatttgcc caaaccctgc acaagctcgg
     8461 tgtcgaaatc ctctccaccg gcggcacggc gaagctgctt gccggtgcgg gcgttcccgt
     8521 gattgaagtc gccgactata ccggtttccc cgaaatgctc gacggccgcg tgaaaaccct
     8581 gcatcctaaa attcatggcg gcatcctcgg tcggcgcgat ttggacgaac acgtcgccaa
     8641 gatggaagaa cacggcatcg gcaacatcga cctcgtgtgc gtcaacctct accccttcgc
     8701 cgccaccatc gccaagcccg gctgcacact ggaagacgcg attgaaaaca tcgacatcgg
     8761 cggcccgacc atggtgcgct ccgccgcgaa aaactggaaa cacgtcgcca tcgttaccga
     8821 caccgccgac ttccccgcca ttgccgccga actcgaagcc ggcaacggcg cgttgagcga
     8881 caaaacccgt ttcaacctct cacgcaaagc attcagccac accgcccaat acgacggcat
     8941 gatttccaac tacctgacct ccctttcaga cggtgttttg agcggccagc cccaaatcgg
     9001 cgaattccca agccggttca accaaagctg gattaaagtg caagacatgc gctacggcga
     9061 aaacccgcat cagcgcgccg cgttttactg cgatattgac cccgccgcag gcagcctttc
     9121 cgcctacaat caactgcaag gcaaagaatt gtcttacaac aacatcgccg atgccgatgc
     9181 cgcttgggaa gccgtcaaat ccttcgacgc gcccgcctgc gtgattgtga agcacgccaa
     9241 tccgtgcggc atcgccgttg cagccgatac cttgaccgcc tacaaactcg cctacgccac
     9301 cgacaccacc agcgcgttcg gcggcatcat cgccttcaac cgcgaagtcg acggcgaaac
     9361 cgtcaaacag attaccgaca accagtttat ggaagtcttg atggcgccga aattcaccgc
     9421 cgaagccctt gaaatcgccg ccgccaagaa aaacgtgcgc gtattggaag tgccgctcaa
     9481 agcaggtgcc aaccgcttcg aactcaaacg cgtcggcggc ggactgttgg tacaaacccc
     9541 cgacatcaac cgcatcaacc gcgccgattt gaaagtcgtc tccaaacgcc aaccgaccga
     9601 gcaggaatgg aacgatttgc tgtttgtctg gaacgtcgcc aaatacgtca aatccaacgc
     9661 catcgtgttc ggcaaaggcg gtcaaaccta cggcatcggc gcaggccaaa tgagccgcgt
     9721 ggacagtacc cgcatcgccg cccgcaaagc gcaagatgcc ggtctcgacc tcaacggcgc
     9781 atgtgccgct tccgatgcct tcttcccatt ccgcgacggc gtggatgtca ttgccgagca
     9841 gggcatcaaa gccatcatcc atccggcagg ctcgatgcgc gatcaggaag ttttcgacgc
     9901 ggcggacgaa cacggcatcg ccatggcggt aaccggcatc cgccacttcc gccattaagg
     9961 cagacgaaca aggcaatgcc gtctgaaggg ttttcagacg gcattttgcg ccatttaccg
    10021 taatcctgaa atcccgtcat tcccacgcag gcaggaatct agatgagaat ccatcctgtc
    10081 agaaataccc gaatcgtcag aaatacccgt gtcatcattc ccgatacacc gtaatcctga
    10141 aacccgtcat tcccgcgcag acagaaatcc ggatttgtcc gcgcggaaac ttatgcgccg
    10201 ccattcccgc gaaggcggga atccggaccg ttcggtttcg gttatttccg ataaattcct
    10261 gctgcttttc attcctagat tcccactttc gcgggaatga cgaaggagtg ggaatccggt
    10321 ttttgagttc cggccatttc cgacaaattg ctttggcatt ggatatttcc tattttaatc
    10381 cactatagtg gattaacttt aaaccagtac gg
//
LOCUS       DAXFZR010000076        10348 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000076 DAXFZR010000000
VERSION     DAXFZR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.077"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..397)
                     /locus_tag="ACFJAS_001720"
     CDS             complement(<1..397)
                     /locus_tag="ACFJAS_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3982907.1"
                     /translation="MRNTVGLDISKLTFDATAIVGKTEHSAKFDNDSKGLDQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTDKQDAK
                     LIAQYCRSAKESELVKRQKPTDEQYRLSRM"
     gene            444..866
                     /locus_tag="ACFJAS_001721"
                     /pseudo
     CDS             444..866
                     /locus_tag="ACFJAS_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            complement(921..1376)
                     /locus_tag="ACFJAS_001722"
     CDS             complement(921..1376)
                     /locus_tag="ACFJAS_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFC3982908.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1632..3002)
                     /gene="ffh"
                     /locus_tag="ACFJAS_001723"
     CDS             complement(1632..3002)
                     /gene="ffh"
                     /locus_tag="ACFJAS_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC3982909.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKIPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
     gene            3222..4028
                     /locus_tag="ACFJAS_001724"
     CDS             3222..4028
                     /locus_tag="ACFJAS_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC3982910.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            4485..6025
                     /locus_tag="ACFJAS_001725"
     rRNA            4485..6025
                     /locus_tag="ACFJAS_001725"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            6126..6202
                     /locus_tag="ACFJAS_001726"
     tRNA            6126..6202
                     /locus_tag="ACFJAS_001726"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6160..6162,aa:Ile,seq:gat)
     gene            6208..6283
                     /locus_tag="ACFJAS_001727"
     tRNA            6208..6283
                     /locus_tag="ACFJAS_001727"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6241..6243,aa:Ala,seq:tgc)
     gene            6630..9521
                     /locus_tag="ACFJAS_001728"
     rRNA            6630..9521
                     /locus_tag="ACFJAS_001728"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            9618..9731
                     /gene="rrf"
                     /locus_tag="ACFJAS_001729"
     rRNA            9618..9731
                     /gene="rrf"
                     /locus_tag="ACFJAS_001729"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            9767..9886
                     /locus_tag="ACFJAS_001730"
                     /pseudo
     CDS             9767..9886
                     /locus_tag="ACFJAS_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
     gene            9988..>10348
                     /locus_tag="ACFJAS_001731"
     CDS             9988..>10348
                     /locus_tag="ACFJAS_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC3982911.1"
                     /translation="MSRGGATPYRFLLIRHIVARCGLLFATLKGKTMKKMFVLFCMLF
                     SCAFSLAAVNINAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPA
                     VLAKLKDQASVGAPAPKG"
BASE COUNT         2564 a         2387 c         2944 g         2453 t
ORIGIN      
        1 tcatccgtga aagcctgtat tgctcgtccg taggcttctg cctctttaca agctcgcttt
       61 ctttcgccga ccggcaatac tgcgctatca gctttgcatc ctgtttgtct gttttggttc
      121 gcttgaacct gctttctgca tacttgctta ttttcagcgg attcactacg taaacgctgt
      181 aatactgcgc gaagtagtcg gcaacttctt cataatagtt tcccgttgcc tccatgcaga
      241 tatgcagatt ctgacatccc aagcttttca accggtccga aaactgatct aaaccttttg
      301 aatcgttgtc aaactttgcc gaatgctccg ttttgccgac aatggccgtt gcgtcaaatg
      361 tcagcttgga tatgtccaat cctacagtgt tacgcatggg attaccctta tatattgcgt
      421 ccctaagaag gggcgcaata tataaggtgt ggcaatttgt cgaagacgcg ctgcgtgccg
      481 tcgtgcctgc cgacagtttt gaaccgaccg cgcaaaaatt gaagctgttt aaggtgggcg
      541 cggcaaccat tttgttttat gaagatcaaa atgtcgtcaa aggtttgcag gagcagttcc
      601 ctgcttatgc cgccaacttt cccgtttggg cggaccaggc gaacgctatg gtacagtatg
      661 ccgtctggac gacacttgcc gcggtcggtg caggtgcaaa tctgcaacat tacaacccct
      721 tgcccgatgc ggcgattgct aaagcgtgga atattcccga aaactggctg ttgcgcgcgc
      781 aaatggttat cggtggtatt gaaggggcgg caggtgaaaa ggtctttgaa cccgttgcgg
      841 aacgtttgaa agtgttcggc gcataatttc gcggtcaaaa aaatgccgtc tgaaccccgt
      901 tcagacggca tttttcagta tcaggcggcg agtttttcgc attctttgat acagtcgagg
      961 caggactcgt aacaggcttt gcattccgca tggtgggcgg agtgttcttt acaggctttg
     1021 gcacactgtt tgcacgcttc gaggcacact tttgccgcgt cgcgtgtcag aggggaattt
     1081 tgtgcggcaa ggtcgtgcac cgcgccgcat aaggcaagca tctggcgcac ggcaaccgca
     1141 cagtcggaca tagacgtgtc gccctgagtg agcagggaca ggcagtgggt caggcaaacc
     1201 tgtccggcat ccagacagtg tgcggcagtt tggcggacgg cggtgtaggc ggatgcggcg
     1261 gcaggctgca tatcgtgatg atggtggtag tcggcgtgtc cgtgcgcacg cgcgaaagag
     1321 gcggcggaag ccaaagagac ggcagcggcg ctgcccaaaa attgacgacg gttcatgatt
     1381 ttttccttta tgtttcaaca tgcaggtccg gtataaggta cacacttacc atactgccct
     1441 ggccggatcg gtacgatttt ctgaataatt gcttgatttt gcaggtgtga aattgtcgca
     1501 cgagcaggca tgttttagct attcggaaat tcgaggaagg gtgcatcaac ctgttttgaa
     1561 atgatgcgca gacatggtaa cagacaaaaa gccgtctgaa aatcgtttta gacggctttt
     1621 gttttcaaac attaaagacc gggaaacatt cccttcattc ccttcatgcc tttggccaag
     1681 cgcatcagtt tgcccaaacc gttgccgctg aacatcttca tcatctgctg catttgttca
     1741 aactgtttga gcaatttgtt cacttcctgc acggtcgtgc ccgcgcccat cgcaatacgg
     1801 cgtttgcggc cggctttgag cagggcaggg ttggcgcgtt ctttaggggt catcgagttg
     1861 atgatggctt ctactttgcc catcgctttt tcagccgtac cttcggggat ttgtttcgat
     1921 atttgaccca gttcgcccgg tattttcgac atcaggtttt ccaaaccgcc catattgcgc
     1981 atttgctgga tttgttcttt aaaatcattg aggtcaaagc ctttgccttt ttgcagcttt
     2041 ttcgccattt tggcggcggc ttcttcatca ataccttttt gaacgtcttc aatcagggtc
     2101 aatacgtcgc ccatgccgag gatacggctg gcaagacggt cggggtggaa aggttcgagg
     2161 ccgttgattt tctcgccgac gccgataaat ttaatcggtt tgccggttac atggcgtacg
     2221 gacaatgccg caccgccgcg cgagtcgccg tccatcttgg tcaatacgac gccgttcagc
     2281 ggcagggctt cattaaatgc ctgagcggta tttaccgcat cctgacccag catggcatcg
     2341 ataacgaaca aagtttcaac cgggttgacc accgcatgaa gcgctttgat ttcgttcatc
     2401 atctcttcat cgattgccaa acggccggcg gtatcgacca tcaatacatc gtaaaaatgt
     2461 tttttggcgt aatcaacggc ggcagttgca atctcaaccg gtttttggtt ggtatcggat
     2521 gggaaaaaat ctacgccgac ctgttcggcc aacaggcgca gctgttcaat cgcggcaggg
     2581 cggtaaacgt cggcggatac caccaaaact tttttcttct gatcgttttt cagcaggcgg
     2641 gcgagtttgc cgacggttgt tgtcttaccg gcaccctgca aaccggccat caaaatgatg
     2701 gcaggcggtg aaaccgataa atccagtgtt ttgttttcct tgcccatcag ttcgactaag
     2761 gcttggttga ccacgccgat aaaggcttgg tcaggcgtca ggctgcccgc cacttcctga
     2821 ccgagggcct tttctttgac gttgttgacg aactctttga cgacaggcag ggcgacatcc
     2881 gcctcaagca gggcgagtcg gacttcgcgc aaggcttctt taatattgtc ttcggtcagt
     2941 ttggcctgcc cccggatgtt tttgaagaca ttgctgaagc ggccggttaa attgtctaac
     3001 atactggtcc ttggtctgaa taagaatagc ttgccccatc aggggcattc tttgttaaaa
     3061 taaaatcaaa ataatttgat gcggcttgtg tgccggacag catatcggca aatccgtcaa
     3121 ggcttgcccg aaacggggat tttacaattc caacgttaaa agttccaata tttcataagc
     3181 ggccgcatac ggcgcaacag tatagataga gaaagtccac catgccgaca gttttcatct
     3241 ttttgacggc ggtttacgca ggattgggtg catttgcatg gcactgccaa cagcaggggc
     3301 gcggccggga ttacccgtgg aagacggaat tgccggtttt gggcgcggca ttgaccgtcc
     3361 acggcgcggc actgctgatg ccggtcattc aagacaaaat catcattatg ggcttcgggt
     3421 attccggcag cctgattgtt tggatgatgc tgtttattta ttttgccggc agcttttttt
     3481 atccgctgcg cggagtgcag ttgctgctgt atccttgcgc cgcactgatg ctgctgtcag
     3541 gtttggtttt cccgggaaaa ttctcgggat atgaaattac cgactttccc tttatgctgc
     3601 atatcggcac atcactgctt gcatacgggc tgttcggcat cgcaacatta ttgtccgttt
     3661 tgagcttgct gctgcaccgg agcctgcacc gcagaaactt ctccaagctc gcaggattcc
     3721 tgccgtcgct gctcagtttg gaaaaactca tgttccaggc catgtgggca ggtttcatcc
     3781 tgctgaccta ttccgtcgtc agcggcacat tcttcgccga agccgtattc ggcaaaccca
     3841 tgacatttac ccataaaacc gtattcggca tattgtcatg gctgatttac ggaggactgc
     3901 tgctcaaaca cagcatgacc gcatggcgcg gcaaaaaagc cgccgtgtgg accatcatcg
     3961 gatttgtcag ccttatgatt gcctatatgg gcagcaagtt cgtattggaa atcattctga
     4021 aaagataaga agagccaaca gatgccgtct gaagccccga gtttcaggcg gcatattcac
     4081 aaaggcgcat cagccggagg agaagaggag ggggggttgg aggcggcgca gcgtttggcg
     4141 gagataaaaa accttatccg acagcgacat gacgaatttc cccaaaaaaa tcccgctgaa
     4201 agtattgact gttttcccct gcgggcgtat agttcggttc ttcgccgctg ccgaagcggc
     4261 ggacgaactg aaaagtatag cacggaatgt cggaaatatc gagagatatc ttagacaggc
     4321 ggaagggata ctttataatt tcgcaacgct ctttaacaaa acagattacc gataagtgtg
     4381 agtgccttgg gcctcgcact gtttgaaaga cagacgagat gatgttttga acattgtcct
     4441 gtcggtttct ttgaagcaga ccagaagtta aaaagttaga gattgaacat aagagtttga
     4501 tcctggctca gattgaacgc tggcggcatg ctttacacat gcaagtcgga cggcagcaca
     4561 gggaagcttg cttctcgggt ggcgagtggc gaacgggtga gtaacatatc ggaacgtacc
     4621 gggtagcggg ggataactga tcgaaagatc agctaatacc gcatacgtct tgagagggaa
     4681 agcaggggac cttcgggcct tgcgctatcc gagcggccga tatctgatta gctggttggc
     4741 ggggtaaagg cccaccaagg cgacgatcag tagcgggtct gagaggatga tccgccacac
     4801 tgggactgag acacggccca gactcctacg ggaggcagca gtggggaatt ttggacaatg
     4861 ggcgcaagcc tgatccagcc atgccgcgtg tctgaagaag gccttcgggt tgtaaaggac
     4921 ttttgtcagg gaagaaaagg ccgttgccaa tatcggcggc cgatgacggt acctgaagaa
     4981 taagcaccgg ctaactacgt gccagcagcc gcggtaatac gtagggtgcg agcgttaatc
     5041 ggaattactg ggcgtaaagc gggcgcagac ggttacttaa gcaggatgtg aaatccccgg
     5101 gctcaacccg ggaactgcgt tctgaactgg gtgactcgag tgtgtcagag ggaggtggaa
     5161 ttccacgtgt agcagtgaaa tgcgtagaga tgtggaggaa taccgatggc gaaggcagcc
     5221 tcctgggata acactgacgt tcatgtccga aagcgtgggt agcaaacagg attagatacc
     5281 ctggtagtcc acgccctaaa cgatgtcaat tagctgttgg gcaacttgat tgcttggtag
     5341 cgtagctaac gcgtgaaatt gaccgcctgg ggagtacggt cgcaagatta aaactcaaag
     5401 gaattgacgg ggacccgcac aagcggtgga tgatgtggat taattcgatg caacgcgaag
     5461 aaccttacct ggttttgaca tgtgcggaat cctccggaga cggaggagtg ccttcgggag
     5521 ccgtaacaca ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
     5581 ccgcaacgag cgcaaccctt gtcattagtt gccatcattc ggttgggcac tctaatgaga
     5641 ctgccggtga caagccggag gaaggtgggg atgacgtcaa gtcctcatgg cccttatgac
     5701 cagggcttca cacgtcatac aatggtcggt acagagggta gccaagccgc gaggcggagc
     5761 caatctcaca aaaccgatcg tagtccggat tgcactctgc aactcgagtg catgaagtcg
     5821 gaatcgctag taatcgcagg tcagcatact gcggtgaata cgttcccggg tcttgtacac
     5881 accgcccgtc acaccatggg agtgggggat accagaagta ggtagggtaa ccgcaaggag
     5941 tccgcttacc acggtatgct tcatgactgg ggtgaagtcg taacaaggta gccgtagggg
     6001 aacctgcggc tggatcacct cctttctaga gaaagaaggg gctttaggca ttcacactta
     6061 tcggtaaact gaaaagatgc ggaagaagct tgagtgaagg caaggttcgc ttaagaaggg
     6121 aaaccgggtt tgtagctcag ctggttagag cacacgcttg ataagcgtgg ggtcggaggt
     6181 tcaagtcctc ccagacccac caagaacggg ggcatagctc agttggtaga gcacctgctt
     6241 tgcaagcagg gggtcatcgg ttcgatcccg tttgcctcca ccaaaacttt acaaatgaaa
     6301 gcaagtttgc tgtttttagc agcttatttt gatttgcgaa gtagaataac gacgcatcga
     6361 tctttaacaa attggaaagc cgaaatcaac aaacaaagac aatgagtttg ttttgatttt
     6421 ttattctttg caaaggataa aaacctctcg caagagaaaa gaaaacaaac atagtatttg
     6481 ggtggtgatt gtatcgactt aatcctgaaa cacaaaaggc aggattaaga cacaacaaag
     6541 cagtaagctt tatcaaagta gggatttcaa gtttgcttac ttagtcaacg ggtaggtaaa
     6601 cgaagtcaaa gaagttcttg aaatgataga gtcaagtgaa taagtgcatc aggcggatgc
     6661 cttggcgatg ataggcgacg aaggacgtgt aagcctgcga aaagcgcggg ggagctggca
     6721 ataaagctat gattccgcga tgtccgaatg gggaaaccca ctgcattctg tgcagtatcc
     6781 taagttgaat acataggctt agagaagcga acccggagaa ctgaaccatc taagtacccg
     6841 gaggaaaaga aatcaaccga gattccgcaa gtagtggcga gcgaacgcgg aggagcctgt
     6901 acgtaataac tgtcgagata gaagaacaag ctgggaagct tgaccatagc gggtgacagt
     6961 cccgtattcg aaatctcaac agcggtacta agcgtacgaa aagtagggcg ggacacgtga
     7021 aatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcatca tcgaccgata
     7081 gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg gaggggagtg aaacagaacc
     7141 tgaaacctga tgcatacaaa cagtgggagc gccctagtgg tgtgactgcg taccttttgt
     7201 ataatgggtc aacgacttac attcagtagc gagcttaacc ggatagggga ggcgtaggga
     7261 aaccgagtct taatagggcg atgagttgct gggtgtagac ccgaaaccga gtgatctatc
     7321 catggtcagg ttgaaggtgc cgtaacaggt actggaggac cgaacccacg catgttgcaa
     7381 aatgcgggga tgagctgtgg gtaggggtga aaggctaaac aaactcggag atagctggtt
     7441 ctccccgaaa actatttagg tagtgcctcg agcaagacac tgatgggggt aaagcactgt
     7501 tatggctagg gggttattgc aacttaccaa cccatggcaa actcagaata ccatcaagtg
     7561 gttcctcggg agacagacag cgggtgctaa cgtccgttgt caagagggaa acaacccaga
     7621 ccgccggcta aggtcccaaa tgatagatta agtggtaaac gaagtgggaa ggcacagaca
     7681 gccaggatgt tggcttagaa gcagccatca tttaaagaaa gcgtaatagc tcactggtcg
     7741 agtcgtcctg cgcggaagat gtaacggggc tcaaatctat aaccgaagct gcggatgccg
     7801 gtttaccggc atggtagggg agcgttctgt aggctgatga aggtgcattg taaagtgtgc
     7861 tggaggtatc agaagtgcga atgttgacat gagtagcgat aaagcgggtg aaaagcccgc
     7921 tcgccgaaag cccaaggttt cctacgcaac gttcatcggc gtagggtaag tcggccccta
     7981 aggcgaggca gaaatgcgta gtcgatggga aacaggttaa tattcctgta cttgattcaa
     8041 atgcgatgtg gggacggaga aggttaggtt ggcaagctgt tggaatagct tgtttaagcc
     8101 ggtaggtgga agacttaggc aaatccgggt tttcttaaca ccgagaagtg atgacgagtg
     8161 tctacggaca cgaagcaacc gataccacgc ttccaggaaa agccactaag cttcagtttg
     8221 aatcgaaccg taccgcaaac cgacacaggt gggcaggatg agaattctaa ggcgcttgag
     8281 agaactcggg agaaggaact cggcaaattg ataccgtaac ttcgggagaa ggtatgccct
     8341 ctaaggttaa ggacttgctc cgtaagcccc ggagggtcgc agagaatagg tggctgcgac
     8401 tgtttattaa aaacacagca ctctgccaac acgaaagtgg acgtataggg tgtgacgcct
     8461 gcccggtgcc ggaaggttaa ttgaagatgt gcaagcatcg gatcgaagcc ccggtaaacg
     8521 gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc
     8581 gcacgaatgg cgtaacgatg gccacactgt ctcctcccga gactcagcga agttgaagtg
     8641 gttgtgaaga tgcaatctac ccgctgctag acggaaagac cccgtgaacc tttactgtag
     8701 ctttgcattg gactttgaag tcacttgtgt aggataggtg ggaggcttgg aagcagagac
     8761 gccagtctct gtggagtcgt ccttgaaata ccaccctggt gtctttgagg ttctaaccca
     8821 gacccgtcat ccgggtcggg gaccgtgcat ggtaggcagt ttgactgggg cggtctcctc
     8881 ccaaagcgta acggaggagt tcgaaggtta cctaggtccg gtcggaaatc ggactgatag
     8941 tgcaatggca aaaggtagct taactgcgag accgacaagt cgggcaggtg cgaaagcagg
     9001 acatagtgat ccggtggttc tgtatggaag ggccatcgct caacggataa aaggtactcc
     9061 ggggataaca ggctgattcc gcccaagagt tcatatcgac ggcggagttt ggcacctcga
     9121 tgtcggctca tcacatcctg gggctgtagt cggtcccaag ggtatggctg ttcgccattt
     9181 aaagtggtac gtgagctggg tttaaaacgt cgtgagacag tttggtccct atctgcagtg
     9241 ggcgttggaa gtttgacggg ggctgctcct agtacgagag gaccggagtg gacgaacctc
     9301 tggtgtaccg gttgtaacgc cagttgcata gccgggtagc taagttcgga agagataagc
     9361 gctgaaagca tctaagcgcg aaactcgcct gaagatgaga cttcccttgc ggtttaaccg
     9421 cactaaaggg tcgttcgaga ccaggacgtt gataggtggg gtgtggaagc gcggtaacgc
     9481 gtgaagctaa cccatactaa ttgcccgtga ggcttgactc tatcatttga aggacttcaa
     9541 gagataaaag cttactgact gattcagtca ttaccgaata tattgattaa ggctttaccg
     9601 atttgcaaca gtttaagttt ggcggccata gcgagttggt cccacgcctt cccatcccga
     9661 acaggaccgt gaaacgactc agcgccgatg atagtgtggt tcttccatgc gaaagtaggt
     9721 cactgccaaa caccctttca gaaaaccccc ggatatccgg gggtttttgc tttgcccgga
     9781 aaaaatgtcg gggatggcgg gacggcatct gtacggtgtc cggtcgggtt tgcggaggaa
     9841 cggcttgaaa ctttgggata ttcattttag aatgacccgt tttatagcgg attaacaaaa
     9901 atcaggacaa ggcggcgggc cgcaggcagt acggatagta cggaaccggt tcgcccggtg
     9961 cttcatcacc ttagggaatc gttccctttg agccggggcg gggcaacgcc gtaccggttt
    10021 ttgttaatcc gccatatcgt cgcaagatgc ggtttgttgt ttgcaaccct taaaggaaaa
    10081 accatgaaaa aaatgtttgt attgttctgt atgctgttct cctgcgcctt ctcccttgcg
    10141 gcggtaaaca tcaatgcggc ttcgcagcag gagctggagg cgctgccggg cataggcccg
    10201 gcgaaggcga aggccattgc ggaataccgc gcgcaaaacg gcgcgttcaa gtctgtggac
    10261 gatttgatca aggtgaaggg catcggtccg gcggtgctgg cgaagctgaa agaccaggct
    10321 tccgtcggcg cgcccgcacc aaaaggcc
//
LOCUS       DAXFZR010000077        10314 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000077 DAXFZR010000000
VERSION     DAXFZR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.078"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            57..1112
                     /gene="aroG"
                     /locus_tag="ACFJAS_001732"
     CDS             57..1112
                     /gene="aroG"
                     /locus_tag="ACFJAS_001732"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC3982912.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAVEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
     gene            1180..1668
                     /locus_tag="ACFJAS_001733"
     CDS             1180..1668
                     /locus_tag="ACFJAS_001733"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC3982913.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKVYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEVALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(1698..2174)
                     /locus_tag="ACFJAS_001734"
     CDS             complement(1698..2174)
                     /locus_tag="ACFJAS_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC3982914.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(2186..2614)
                     /locus_tag="ACFJAS_001735"
     CDS             complement(2186..2614)
                     /locus_tag="ACFJAS_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC3982915.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            2726..2971
                     /locus_tag="ACFJAS_001736"
     CDS             2726..2971
                     /locus_tag="ACFJAS_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982916.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            3498..3911
                     /locus_tag="ACFJAS_001737"
     CDS             3498..3911
                     /locus_tag="ACFJAS_001737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982917.1"
                     /translation="MSFDCFGAFSYQPGSDTRPTNVGDTADFSFSDKPANGVFHYFSS
                     GKTDQKSSEALYDEINITDNYRTDLKQAVKKQLQDLYKTRPEAWEENKKRVREEIIKQ
                     FGISEDVFNKLQQKMPQKINSLIEEQVLTPYILRP"
     gene            complement(4008..4169)
                     /locus_tag="ACFJAS_001738"
     CDS             complement(4008..4169)
                     /locus_tag="ACFJAS_001738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982918.1"
                     /translation="MQNIKSGKRLKIDTVTQSKTGFCPNAGADLPLTAAEQPDRRPSL
                     SVKPPFTPK"
     gene            4095..4352
                     /locus_tag="ACFJAS_001739"
     CDS             4095..4352
                     /locus_tag="ACFJAS_001739"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC3982919.1"
                     /translation="MRAKTRFALRNGVNLQPFAAFNVLHRSKSFGMEMDGEKQTLAGR
                     TALEGRFGIEAGWKGHMSARIGYGKRTDGDKEAALSVKWLF"
     gene            4594..6063
                     /locus_tag="ACFJAS_001740"
     CDS             4594..6063
                     /locus_tag="ACFJAS_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3982920.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLA
                     AALFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGLSVTREHN
                     INQAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRVYPGNKK
                     FNDMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTL
                     SSAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNR
                     YGLRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRA
                     LHFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(6264..7232)
                     /locus_tag="ACFJAS_001741"
     CDS             complement(6264..7232)
                     /locus_tag="ACFJAS_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982921.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            7309..8601
                     /locus_tag="ACFJAS_001742"
     CDS             7309..8601
                     /locus_tag="ACFJAS_001742"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC3982922.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGGTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(8951..9637)
                     /locus_tag="ACFJAS_001743"
     CDS             complement(8951..9637)
                     /locus_tag="ACFJAS_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC3982923.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(9712..10185)
                     /gene="queF"
                     /locus_tag="ACFJAS_001744"
     CDS             complement(9712..10185)
                     /gene="queF"
                     /locus_tag="ACFJAS_001744"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC3982924.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(10189..10233)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         2485 a         2563 c         3016 g         2250 t
ORIGIN      
        1 caaggtaaac gatgtaacct aagccgcgta taaaaaccca tcaggaaaga tgcaagatga
       61 cacaccatta ccccacagac gatattaaga ttaaagaagt taaagagttg ttgccgccga
      121 ttgcccatct ttacgagctg ccgatttcca aagaggcttc gggcttggtt caccgcaccc
      181 gtcaggaaat ttccgatttg gttcacggca gggacaggcg gctgttggtc attatcgggc
      241 cgtgttcgat tcacgatccg aaagcggcgt tggaatatgc ggaacgtctg ttgaaactcc
      301 gcaagcggta tgaaaacgag cttttgatcg tgatgcgcgt ttatttcgag aagccgagaa
      361 cgacggtggg ttggaaaggt ttgattaacg acccgcattt ggacggtacg ttcgacatca
      421 atttcggttt gcgtcaggcg cgcagcctgt tgttgtccct gaataatatg ggcatgcctg
      481 cctctaccga atttttggat atgattacac cccagtatta tgccgacctg atttcttggg
      541 gggcaatcgg cgcgcggacg accgaaagcc aagtccaccg cgaattggca agcggtctgt
      601 cctgccccgt cggctttaaa aacggtacgg acggcaattt gaagattgcg atcgacgcaa
      661 tcggtgcggc gagccattcg catcatttct tgtctgtaac caaggccggg cattccgcca
      721 ttgtccatac cggcggcaat cccgactgtc atgtcatttt gcgcggcgga aaagagccga
      781 attatgatgc gggacacgtc agcgaggcgg tggaacaact gcgtgcggca ggggtaaccg
      841 acaagctgat gatcgattgc agccacgcca acagccgcaa ggattacacc cggcagatgg
      901 aagtggcaca agacattgcc gaccaattgg aacaggacgg cggcaatatc atgggcgtga
      961 tggtggaaag ccatttggtc gaaggcaggc aggacaagcc ggaagtgtac ggcaagagca
     1021 ttaccgatgc gtgtatcggt tggggcgcga ctgaagaatt gttggcattg ttggcgggtg
     1081 cgaacacaaa acgtatggcg cgcgcaggtt gagatttttg acgcagaatg tcataaaatg
     1141 ccgtctgaag cgttcagacg gcatttttgt ggagaaaata tgctcaaaat aaccataatt
     1201 gcggcgtgtg cggaaaacct gtgcatcggg gcgggcaatg ctatgccttg gcacatcccc
     1261 gaagatttcg catttttcaa agtctatacc ttgggcaaac ccgtcattat ggggcggaaa
     1321 acgtgggaat ccctgcccgt caaacccctg cccggacgga ggaacatcgt catcagccgg
     1381 caggcggatt attgcgcggc aggcgcggaa acggtggcaa gtttggaggt ggcattggca
     1441 ttgtgcgccg gcgcggaaga agccgtcatt atgggcggcg cgcagatata cggacaagcg
     1501 atgccattag cgaccgattt gcggataacc gaagtggatt tgtctgtgga aggagatgca
     1561 tttttcccag aaatagaccg gacgcattgg aaagaagcag agcggacgga acgccgtgtc
     1621 agcagcaaag gcgttgcata tacattcgtg cattacctca agggataacc gcaaggcaga
     1681 tgccgtctga agccgcttca gacggcattt ttccagcttg agaacggatg tttgctcaaa
     1741 taggcattgg taaacatacc gtctgaagtg atttcgcgcc ccagccagtc cggacggtca
     1801 aaatcggcat tttcggcggg caactcgatt tccgcgacga ccaaaggcga gttttcgcca
     1861 aggaaaacat cgacttcaaa caggctgccg ccccatctga ccggataacg ccgtttttcc
     1921 atcttaaacg ggcacatcgt ttccatcatc ttttccgcat cggcaagcgg gatttcgtat
     1981 tcgaactcgc tgcggctgat ttccgaaata tagcctttca gcgtcagcca cgcccgtttt
     2041 ccggcaatgc ggatgcggat ggtgcgttct ttttcaacag acaggtagcc ctgtttcagc
     2101 agtagcggct catcggcgta ttgccgccat ttgtcgtttt caatcaaaaa acggcgttcg
     2161 atttctatcg gcataagatg ctccgtcaaa acggtttgaa cacgaccaga tacagcgcgg
     2221 caaccatcag cagcacgggg atttcgttga acacgcggta ccagcggtgt gaaaaagcat
     2281 tgctgtaatc ctgaaaacgg cgcagcagca cgccgcaata caactgataa gccaagagca
     2341 tcaagcccaa acacagtttg acgtgaaccc agccgctgcc ccaccggccg gcggcaaacg
     2401 gtatcgccgc gccgaacacg accgcgccga aacccaaagg cgacataaaa cggtacaacc
     2461 gcaccgccat ccccgacagg cgcacatact cgggattgcc gcgcggcgca tcaatcatcg
     2521 ccatattgac gaaaatcctc ggcaggtaaa acagccctgc aaaccacgaa atgacaaaaa
     2581 acaagtgaaa cagcttgaac caagaaaaca tcatcgccca cacccttccg aaaagcggta
     2641 ttgtacaggc aaaccgcttg ggaaacgtga taaaatcagg cggataaaca aatcgaataa
     2701 atccttaccg caaaacggag gcaaaatgct caaatccatc gaactcaatt cacacatccg
     2761 caaccgcctt gcagcatatc tgaaaggcag gggtttggat tttcagacgg caatgcagga
     2821 agaagaaggc aacaaagaaa tcgccgccat cgtccacagc ggtctgccca ctctggtccg
     2881 caaactgtat tccgaacaaa aaatgcagaa gtttttttgg gaaaagcggg atttgattgc
     2941 cgactacatc agccgccgga tgcagggata ggcggctgaa atctgttttc aggcaagtga
     3001 aaagacaata tggcggattg aaattacgct tatcgtcatt cccgcccgcg cgggaatccg
     3061 acttgtccgg tttcggttct ttttctcgtt tcgggtgatt tctaaaccgt cattcccgcg
     3121 caggcgggaa cccagacctt taaaccccgg ccatccccga taaattcttg ccgcattaaa
     3181 attctagatt cccgctttcg cgggaatgac ggcggagggg ttttttgttt ttcccgattt
     3241 ttcattgcga tgtagtgtga tatagtatat aatcattata actttaacac ttgacaaagg
     3301 aaaatttctc atgacactga aagcaagcaa gcggtcgggt taatctatta acattatctg
     3361 ttttatcgct gttttgcacg ccatatgctt ggggttcgga tgcgtacgat cccgtcaaag
     3421 aagccgagat taaaaacaaa tttattttag aagcggcgga agacagaaat tcccacgttt
     3481 ggcgcggccc gtgcagtgtg tcttttgatt gcttcggtgc tttcagttat cagcccggtt
     3541 cggatactcg tcctacaaat gtcggcgata ctgccgattt ttcattttca gacaagccgg
     3601 caaacggtgt tttccattat ttctccagcg gtaaaaccga tcaaaaatca tctgaagcct
     3661 tgtatgacga aatcaatatt acagataatt accgaactga tttaaagcag gcagttaaaa
     3721 agcaattaca ggatttatac aaaacaagac ccgaggcttg ggaagaaaat aaaaaacggg
     3781 ttagggaaga gataataaag cagtttggaa tttctgaaga tgtgtttaat aaacttcaac
     3841 agaaaatgcc tcaaaagatt aattctttaa tagaagaaca ggtactcact ccttatatat
     3901 tgcgtcccta agaaggggcg ttaatttttg ttaatcgccc cttcttaggg acgcaatata
     3961 taaggcagta atatgcagtt ttacctgcaa ccgcaggcgc agtttaccta cttgggcgta
     4021 aacggcggct ttaccgacag cgaggggcgg cggtcgggct gctcggcagc ggtcagtggc
     4081 aaatccgcgc cggcattcgg gcaaaaaccc gttttgcttt gcgtaacggt gtcaatcttc
     4141 agccttttgc cgcttttaat gttttgcaca ggtcaaaatc tttcggcatg gaaatggacg
     4201 gcgaaaaaca gacgctggca ggcaggacgg cgctcgaagg gcggtttggc attgaagccg
     4261 gttggaaagg ccatatgtcc gcacgcatcg gatacggcaa aaggacggac ggcgacaaag
     4321 aagccgcatt gtcggtcaaa tggttgtttt gatgcgccgg gaaatgtttt gacacacagg
     4381 cggcacacct gcacggtccc gtgcgccgcc ccgcaaaccg atccgaaccc tgccgcccag
     4441 aagggcgggg cataatgatg aaaccggcgg aaaaccgccg gttttttgcc gccgtctgaa
     4501 acccgattct ggcttcagac ggcattgtcg cggcatcggg tggcagggtt tggaacagcg
     4561 gcataaaaaa ctgatacaat ccgccggttg ataatggtta ttttttattt ttatttttgt
     4621 gggaagacat ttatgcctgc acgaaacaga tggatgctgc tgcctttatt ggcaagcgcg
     4681 gcatacgccg aagaaacacc gcgcgaaccg gatttgagaa gccgtcccga gttcaggctt
     4741 catgaagcgg aggtcaaacc gatcgacagg gagaaggtac cggggcaggt gcgggaaaaa
     4801 ggaaaagttt tgcaggttga cggcgaaacc ctgctgaaaa atcccgaatt gttgtcgcgt
     4861 gccatgtatt ccgcagtggt ctcaaacaat attgccggta tccgcgtgat tttgccgatt
     4921 tacctacaac aggcgcggca ggataagatg ttggcacttt atgcacaagg gattttggcg
     4981 caggcagagg gcagggtgaa ggaggcggtt tcccattacc gggaattgat tgccgcccaa
     5041 cccgacgcgc ccgccgtccg tatgcgtttg gcggcggcat tgtttgaaga caggcagaac
     5101 gaggcggcgg cagaccagtt cgaccgcctg aaaacagaag atctgccgcc gcagcttatg
     5161 gagcaggtcg agctgtaccg caaggcattg cgcgaacgcg atgcgtggaa ggtaaacggc
     5221 ggtctcagcg ttacccgcga acacaatatc aaccaagccc cgaaacagca gcagtacggc
     5281 aattggactt tcccgaaaca ggtggacggc acggcggtca attaccggtt cggcgcggag
     5341 aaaaaatggt cgctgaaaaa cggctggtac acgacggcgg gcggcgacgt gtccggcagg
     5401 gtttatccgg ggaataagaa attcaacgat atgacggcag gtgtttccgg cggcatcggt
     5461 tttgccgacc ggcgtaaaga tgtcgggctg gcagtgttcc acgaacgccg cacctacggc
     5521 aacgacgctt attcttacgc caacggcgca cgcctttatt tcaaccgttg gcaaaccccg
     5581 agatggcaaa cgctgtcttc ggcggagtgg gggcgtttga agaatacgcg ccgggcgcgt
     5641 tccgacaata cccatttgca aatttccaat tcgctggtgt tttaccggaa tgcgcgccaa
     5701 tattggacgg gcggtttgga tttttaccgc gagcgcaacc ccgccgaccg tggcgacaat
     5761 ttcaaccgtt acggcctgcg ctttgcctgg gggcaggaat ggggcggcag cggcctgtct
     5821 tcgctgttcc gcctcggcgt ggcgaaacgg cattatgaaa aacccggctt cttcagcagt
     5881 tttaaagggg aaaggcgcag ggataaagaa tcggacacat ccttgagcct ttggcaccgg
     5941 gcattgcatt tcaaaggcat cacgccgcgc ctgacgctgt cgcaccgcga aacgtggagc
     6001 aacgatgtgt ttaacgaata cgagaaaaac agggcgtttg tcgagtttaa caaaacgttc
     6061 tgattgctgt tccttttcgg cggaaaccct gccggcggcg gtgtcacggc gggcatcggc
     6121 ggctttcggg cggtgttttg cgtgccgccg cgtgtgcgga aacgtattcc ggtttttccg
     6181 gcataacggc gatgcggggt aaaatgccgt ctgaaacccg attcgggctt cagacggcat
     6241 tgtcgcggtt gcggcgggcg ggtttaccag attccgtcaa aggttttcgt gcagcgccaa
     6301 aacttccgcc tgtctgtggg tttgaaggtc agcgtaccgc cgtgttgtcc gtccgtggcg
     6361 atgtccagcc gtttgatttt gccggtgcgg acggcttcgt aaagcggtgc gaaccagcgt
     6421 tcttcccact gctgcaatat tgccgcatac cgctccctgt cccctgtcag ggcggtcagg
     6481 cgcaaatcgt ccataaacag gatgtggtgc gtatcgggca ggtgtgccgc cgtttccgca
     6541 taagctcgga agctgtcggg caatgctcgg cggttcgggt gaaaacggct ccaaaccgta
     6601 tcggcgaaaa gcgtgccgcc ttgtgcgctt ccgtgcagac tgtcccaaag ccataagccg
     6661 ttcaattcgg gcagcccgcg ttttttgcgg ttgtggttga cggggtgagc gttcagccac
     6721 atttggattt cggtttggac gcgcagccat tccaacgcgt cttccccgtc cggctgatcg
     6781 tccgcaccca acagtccgcc caagtccaaa acgggcttcg cgccccagcg gtacgcgcgg
     6841 ggaagggaaa ccagccataa ttcgggcagg acgggaacga aacgccacgg aatgtcgccg
     6901 taaaacgccg acagatcgcg gcagagccgt tccgcttcat ccgtaccgac atccaaatat
     6961 tccgccgtca gcacatttgc ctgatgcagc cccatttttt gccagacggg cgtggcgagc
     7021 gcgacggttt cagacggcat attcaggctt tgcgccgggc gttgtgccag tcttccgcac
     7081 cacaaataac gtgcgtaaaa tgccgaagcc gtgcagcttt ggcggtgcgg cgagccgtat
     7141 tgcaggattt tgttgaaggc gggcaggcat agaggtattc ggatttcgtc ttcatccaaa
     7201 ttgagcgagg ggagggcgag ggtgagtttc atcgtttgat gcctgacaaa tgaggatcgg
     7261 gcggatatta tagaggattc ggcgcaaacg ccgtcaaaaa ggaacaatat ggctgtcttc
     7321 ccactttcgg caaaacatcg gaaatacgcg ctgcgcgcgc ttgccgtttc gattattctg
     7381 gtgtcggcgg catacattgc ttcgacagag gggacggagc gcgtcagacc gcagcgcgtg
     7441 gaacaaaaac tgccgccgct gtcttggggc ggcagcggcg ttcagacggc atattgggtg
     7501 caggaggcgg tgcagccggg cgactcgctg gcggacgtgc tggcgcgttc gggtatggcg
     7561 cgggacgaga ttgcccgaat cacggaaaaa tatggcggcg aagccgattt gcggcatttg
     7621 cgtgccgacc agtcggttca tgttttggtc ggcggcgacg gcagtgcgcg cgaagtgcag
     7681 ttttttaccg acgaagacgg cgagcgcaat ctggtcgctt tggaaaaaaa aggcggcata
     7741 tggcggcggt cggcttctga tgcggatatg aaggttttgc cgacactgcg ttcggtcgtg
     7801 gtcaaaacgt cggcgcgcgg ttcgctggcg cgggcggaag tgcccgtcga aatccgcgaa
     7861 tccttaagcg ggatttttgc cggccgcttc agccttgacg gtttgaagga aggcgatgcc
     7921 gtgcgcctgc tttacgacag cctgtatttc cacgggcagc aggtggcggc gggcgatatt
     7981 ttggcggcgg aagttgtcaa gggcggcaca acccatcagg cgttctatta ccgttcggac
     8041 aaggaaggcg gagggggcgg caattattac gatgaagacg gcagggtgtt gcaggaaaaa
     8101 ggcggcttca acatcgagcc gctggtctat acgcgcattt cttcgccgtt cggctaccgt
     8161 atgcacccca tcctgcacac atggcggctg cacacgggca tcgattatgc cgcaccgcag
     8221 ggaacgccgg tcagggcttc cgccgacggc gtgattacct ttaaaggccg gaagggcgga
     8281 tacggcaacg cggtgatgat acgccacgcc aacggtgtgg aaacgctgta cgcgcacttg
     8341 agcgcgtttt cgcaggcaca aggcaatgtg cgcggcggcg aggtcatcgg ttttgtcggt
     8401 tcgacagggc gttcgaccgg gccgcacctg cattacgagg cgcgcatcaa cgggcagccc
     8461 gtcaatcctg tttcggtcgc attgccgaca cccgaattga cgcaggcgga caaggcggcg
     8521 tttgccgcgc agaaacagaa ggcggacgcg ctgcttgcgc gcttgcgcgg cataccggtt
     8581 accgtgtcgc aatcggattg aagtttgaac cggcgacgaa aacaatgccg tctgaaaatc
     8641 tgcaaacagg ttttcagacg gcatttatag tggcttaaaa taaaactaag ccaaggaagc
     8701 gctgccgtca ttcccgtacc ggcgggaatc cggacaccac ggcacggaaa cccatccgcc
     8761 gtcattcccg cgaaagcggg aatctagaaa tacaatgcgg caggagttta tcggaaatga
     8821 ctgaacccca acgcaccgga ttcccgcttt cgcgggaatg acgaagtggg tgggaatccg
     8881 gatttatccg ttccggcagt gtttgcaaat aaaagaaaac ccaaccgtcc cgattcccta
     8941 cagagctgtt ttacggattt tgcagcgagg gcacggggcg gtcttgcgcc tgtttggttt
     9001 gcagggccgt cagttttttc gtcagcagat tcagtatcac gccgtaggcg ggcaggaaga
     9061 agagggtgca gacggtaagt ttgaacaggt aatcgacaaa agcgatgccc tgccagtttg
     9121 ccgccataaa ttcatcgctg cttgcgtaaa aggcaacggc aaaaaatact aacgtgtcca
     9181 gtgcattgcc gatgacggtt gatgcggtcg gggcaatcca ccacgctttc agacggcgta
     9241 atttgtcgaa tacgaaaata tcaaggattt gtccgagcgc gtaggcggca aaacttgcca
     9301 gcgcgatgcg tccgacaaag gtgttgaatt gggacagcgc gcccaagccc gtccaactgc
     9361 cgttgtggaa caaaacggaa aagacgtagg aaagcaaaag ggcggggaac atcacccaaa
     9421 agataatccg ccgcgccaag tgcgaaccga aaatgcggac ggtcaggtcg gtggcgagga
     9481 agatgaaggg aaaggaaaac gcgccccaag tggtgtggat gccgaaaatc cggaagggga
     9541 actgcaccag atagttgctg gcggcgatga tgaggatatg gaaaagcacc agccggaaga
     9601 gtgccttctg ttgctgtgcg gcggtcaatg cgtacataaa aacctttcgg aaaggcgttc
     9661 agacggcata ccgtatcgaa ggaatgccgt ctgaaatatg ggaaggatgg tttattgtgc
     9721 gtcgtgctcg aacaggcgtt tgcgtgccaa tgcttcaaac tctgtgcctg ctttgccgta
     9781 gttggcaaac ggatgaacgg cgatgccgcc gcgcggtgtg aactcgccga acacttcgat
     9841 gtatttcgga tccatcaggg cgatgaggtc tttcatgatg atgttgacgc agtcttcatg
     9901 aaaatcgccg tggttgcgga agctgaagag gtagagtttc agggatttgc tttccaccat
     9961 tttgatgtgc gggatatagc ggatgacgat ggtggcgaag tcgggctgcc cggtcatcgg
    10021 gcagaggctg gtgaattcgg ggcagacgaa tttgacgaaa tagtcgttgt cgggatgttt
    10081 gttgtcgaat gcttcgagaa tttcaggcgc gtagccggtc ggatattggg ttttttgatt
    10141 acccaaaaga gagatgcctt gcagctcttc gttgttgcgg gacatagggt ttccttagtt
    10201 ttttagtgtg ggaggttttc gaaccacggg gcggcgattg taatataagc ggcggtattt
    10261 gtgtagtttt cttcagacgg catggtgcgg gcggcggtgt ttgccatgtc atat
//
LOCUS       DAXFZR010000078        10290 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000078 DAXFZR010000000
VERSION     DAXFZR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.079"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..750
                     /locus_tag="ACFJAS_001745"
     CDS             <1..750
                     /locus_tag="ACFJAS_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFC3982925.1"
                     /translation="ENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLP
                     EVDALFGVPQRADYHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDI
                     LPRHHGHDINGVEPVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLK
                     KTDAFRRPERFQTALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAEC
                     RAQGKAHFIAEQIDRARLAQIAPLQKAFRGA"
     gene            1169..1477
                     /locus_tag="ACFJAS_001746"
     CDS             1169..1477
                     /locus_tag="ACFJAS_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982926.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            1559..2590
                     /gene="ruvB"
                     /locus_tag="ACFJAS_001747"
     CDS             1559..2590
                     /gene="ruvB"
                     /locus_tag="ACFJAS_001747"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC3982927.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMGEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(2835..3518)
                     /gene="rpe"
                     /locus_tag="ACFJAS_001748"
     CDS             complement(2835..3518)
                     /gene="rpe"
                     /locus_tag="ACFJAS_001748"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC3982928.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            3664..4098
                     /locus_tag="ACFJAS_001749"
     CDS             3664..4098
                     /locus_tag="ACFJAS_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC3982929.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(4111..4974)
                     /locus_tag="ACFJAS_001750"
     CDS             complement(4111..4974)
                     /locus_tag="ACFJAS_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3982930.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(4982..5599)
                     /locus_tag="ACFJAS_001751"
     CDS             complement(4982..5599)
                     /locus_tag="ACFJAS_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC3982931.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(5762..6376)
                     /locus_tag="ACFJAS_001752"
     CDS             complement(5762..6376)
                     /locus_tag="ACFJAS_001752"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC3982932.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRPQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            6583..8352
                     /locus_tag="ACFJAS_001753"
     CDS             6583..8352
                     /locus_tag="ACFJAS_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFC3982933.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            8349..9005
                     /locus_tag="ACFJAS_001754"
     CDS             8349..9005
                     /locus_tag="ACFJAS_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC3982934.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(9206..10210)
                     /locus_tag="ACFJAS_001755"
                     /pseudo
     CDS             complement(9206..10210)
                     /locus_tag="ACFJAS_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_304669545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2450 a         3010 c         2476 g         2354 t
ORIGIN      
        1 gaaaacggcg aagcggacgc attggttgcc gaacgcgtct ggcaggagtt ggcgaaaggt
       61 ttgatggaaa aaaatccgcg caaaatgatt gaaatgttgc gcgaatgcgg cgcactccaa
      121 gtcttgctgc ccgaagtcga cgccctcttc ggcgtgccgc aacgcgccga ctatcatccc
      181 gaaatcgaca gcggcatcca taccctgatg acgctgcaac gcgccgccga tatgggtttg
      241 agcctgcccg aacgctatgc cgccctgctg cacgacttgg gcaaagccaa aacaccgccc
      301 gacatcctgc cgcgccacca cggacacgac atcaacggcg tagaacccgt gcgcgaagtc
      361 aatcaacggc tgcgcgcgcc gaggcagtgt gccgagcttg ccgaattggt ttgccgttgg
      421 cacattattt tccaccaagt cggacagctt aaaagccaaa ccattctgaa cgtattgaaa
      481 aaaaccgatg ccttccgccg tcccgagcgt tttcagacgg cattgaacgt ctgcattgcc
      541 gacacgcaag gccgtctgaa ccgagaacac acgccctacc cccagcgcgc gcactggctc
      601 gccctgctcg aagccgccaa tcaggcggat tcgggcaaaa tcgccgccga atgccgcgcg
      661 cagggaaaag cgcactttat cgccgaacaa atcgaccggg cgcggctggc gcaaatcgcc
      721 ccgctgcaaa aagcgtttcg gggcgcgtag gaaaaagaaa acgccaaacg cggcatcttt
      781 tgaggttcgc ccgcattgcc gaaacagcgt cggagaaagg gatggatgac atcggtttat
      841 tcggctgcct tttaatattc ccttgatgca ggcctgatta gtcggacaaa aatacctttt
      901 gttcaacgga accgatttct atccggacgg aaacagccgg ctttcccctt gcaaacggat
      961 ggaatcaagc gggtattcaa acacagccgt ttatttttat tccgccgcca agttatggga
     1021 tagggtttgc gtgtttcggg caggcaaacc gcgcaacctt agacgcagtc cgaagttttg
     1081 aaggcggcat ccgggccggg ttttaaaaca gccgccgcca tatccgctat aatccatcct
     1141 tcagccattc cgcccccgac ataaaatcat gaccctgaaa accgatttat tgcctaaaat
     1201 caacaacgaa gattatcaac gcctcatcct caaacatagc gcggaattca gcggcggaga
     1261 aacccgcctg ttgaacgaaa tcctcgaaaa attcaatttc gacgttgttc aggcgcaggc
     1321 attggcgcag gcggtaatgc agcaagttcg cttcgatccc aacgcctacc acatcgacag
     1381 cgacgacgaa gacaccaccg gcatctgccc ccactgcatc aacccgccta tgccgcccct
     1441 gcgcgactat ctcgtttggc gcgaaacacg cggataaaac gcttttgacc gttatctttt
     1501 caatgccgtc tgaaacgccg ccgaccgttc ggacggcata cccgacaaag ggaacactat
     1561 gctgcaaacc gacaacctga ccgccgcgca accgcaacgc atcgttgccg cccaaaccgc
     1621 ctccgcacag gaagaactgc tcgaacgcgc cctccgcccc aaaacgctgg acgactacat
     1681 cgggcagcat aaagccaaag agcagctcgc catcttcatc caagccgcca aaaaacgcgg
     1741 cgaagcactc gaccacgtcc tgcttttcgg cccgcccgga ctgggcaaaa ccacccttgc
     1801 ccacatcatc gccaaagagt tgggcgtaaa tttgcgccaa accagcggcc ccgtcctcga
     1861 acgcgccggc gacctcgccg cccttttgac caaccttgat ccgcacgacg tattgtttat
     1921 cgacgaaatc caccggctaa gccccgtcgt cgaggaaatc ctctatcccg cgctcgaaga
     1981 ctaccggctc gacattatga taggcgaagg acccgccgca cgttccgtca aaatcgacct
     2041 gccgcccttc accctcgtcg gcgcgaccac ccgcgccggt atgctgacca acccgttgcg
     2101 cgaccgcttc ggcatcgtct cccgcctcga gttttacgaa aaccgcgacc ttaccaccat
     2161 cgtcagccgt tcggcacaac tgttgcagct cgatatgggc gaagaaggcg cgatggaagt
     2221 cgccaaacgc agccgcggca cgccccgcat cgccaaccgc ctgttgcgcc gcgtgcgcga
     2281 tttcgccgac gtgaaaaaca acggcgtaat cgatgccgcc gtcgccgatg ccgctttaag
     2341 tatgctggac gtggacgcgc aggggctgga cgtgatggac aggaaattcc tcgaagccgt
     2401 tttgcacaaa ttcggcggcg gccccgtcgg tttggacaac gttgccgccg ccatcggcga
     2461 atctacggac accatcgaag acgttatcga accctacctc atccaacaag gctttttgca
     2521 acgcaccccg cgcggcagga tggcgaccga acgcgcctac ctgcatttcg ggctgcccgt
     2581 cgaaaaataa accatgccgt ctgaaaccga accgggtttc atacggcatt gttatcagac
     2641 gtttgcaccg gcaccggttt catcacttaa cagaaacctt ttgtccggca ggacagattc
     2701 tccccgatga aaataactgt ttccctggca aacggttgaa acccgtctgg ctgccggttg
     2761 aaaaataacg caatgctgtc tgaaacagag ctaattttca gacggcattt ctatttcaat
     2821 cattggctca aggttcagcc tgccgctttt tccagttccg ccctcatcgc agcgattacc
     2881 gccttgtaat ccggtttgcc gaaaatcgcc gaaccggcaa caaaggtatc cgcaccggct
     2941 cgggcaacgg cggcaatatt gtcagttttg atgccgccgt ccacttcaat ggcaatacgt
     3001 ctgccgcttt tcccttcata gcggtcaagc atggcgcgaa cctgtctgat tttttccaaa
     3061 gtgtgcggaa tgaagctctg accgccgaac ccggggttga ccgacatcag caaaaccata
     3121 tccagcctgt ccaacacgtt ttccaacaca tatacgggcg ttgccggatt caacaccagc
     3181 cccgcctgac agcccatgtc ccgaatcagg ctcaagctgc ggtcgatatg gcggctcgct
     3241 tcgggatgga aagtgataat cgatgctcct gctttggcaa acgattgaat taaatcgtca
     3301 acgggttcga ccatcagatg cacatcgatc ggcacgcttg cataaggctt caacgccgcg
     3361 cagaccatag gtccgaaggt caggttcggc acataatggt tgtccatcac gtcaaaatgg
     3421 atcagatccg cacccgccgc aatgacattt gccacctctt ctccgaggcg ggcaaagtct
     3481 gccgacaaaa tactgggtgc gatacggtag gcggtcatat tttttccttc aatgccttta
     3541 tgggcggact caacgcggga aagcctcgaa acagcattat taacaaaagt taactgccat
     3601 aataccatct ttcacacggc aatcaagtat attgtccgaa atatccggat taaaggaaaa
     3661 gccgtgccgc tgcctgctcc ctgccgtttt gccaaacctg ccgcctcttt tttaagtatg
     3721 gctttgcttt cctgccagct ttcccacgcc gccacggctt atatcccccc gaacgatttt
     3781 caaccgaact gcgacatacg ccggctcggg ctgacacagg gtcagcacaa tgagctgcgt
     3841 aaaatccgcg ccgccttcaa aatggcgggc gacagggcgc gtttgaaggt tatgcattcc
     3901 gaacacagcc gccgccgctc tgtcgtcgaa atcatttctt cggatgtttt taatcggaac
     3961 gaggcgcgcg attatgtcga aagccgctac cactccagca tggattttgc ggtggacgaa
     4021 ttggaaatcc aacaccgctt cttccatatt ctcacaccgc aacagcagca aatgtggctt
     4081 tcttcctgcc tcaaataatc cccgaaacac tcacggcgca cgttgctccg gcagcctgcc
     4141 cgcccagtcg caggcaaact gccaagcgga acgtcccgag cggttgcccc gcatctgcgc
     4201 ccactgcaac gccgccgtcc gggcggcttc gtcatatggc acatcaaaat cttccaacca
     4261 gcttcgcact gccgccagat aatcgttttg atcgaacgga taaaaactga gccacaatcc
     4321 gaatcggtcg gacaaagata ctttttcttc cacggcctct ttttgatgga tttctccgcg
     4381 cacgcctgtc gtacctgcat tttcatccaa atattcgggc atcagatgcc gtctgttgga
     4441 agtcgcgtac accatcacgt tggcgcaacg ttgggacaga ccgccatcca atgccgtttt
     4501 caatgcctta taggtttcgt cgccgctttc aaatgacaaa tcgtcgcaaa acacaatgaa
     4561 tttttccggg cgttccttca aaagtgtcaa cagatacggc aggccgatca aatcgctttt
     4621 atcgacttcg atcaggcgca atcccttatc cgcatattcg tgcagcaggg ctttgaccag
     4681 cgaggatttg cctgttccgc gcgcgccgct catcaatacg ttattcgcag gtctgccggc
     4741 aataaattgt tcggtattac gcaccagcaa ttcggtctgc ctgccgactc cggcaagccg
     4801 cgacaaaggg aaaacgtgcg gatcgggcaa gtgttccaaa aaaccttttt tgcccgcact
     4861 ctgccagcgg aaggcaagcg cgttccaatc cgtatgcccg ggttcgggcg gaagcacggc
     4921 atccaaacgc cgcaaaacgg cataggcttt atcgagaaat tcgttcaatt ccatctctac
     4981 ctcactttgc atatctttgc gccatcagcc gttcgacggt gtcgacgatt gcctgcgtat
     5041 tcggatcgat ttcgatgttg atcctgtctc cggcctttct gctgccgaac agcgtccgtt
     5101 ccaaagtttc gggaatcaga tggacattga aacggctgtc ttcgactttg cctatggtca
     5161 ggctgcaacc gtccaagccg acgaaccctt tggtcaggat atagggtttg agttcgtgcg
     5221 ggagcgcaaa ccaaaccgtg cggttgaacc cgtcccgttc gatttcgaca atgggcacgg
     5281 ttgccataat gtgtccgctc atgacgtgtc cgccgatttc gtcgccgaaa cgtgccgccc
     5341 gttcgatgtt gacgcaatcg ccttccttca gcagccccaa attggttttt gccaaagttt
     5401 ctgccattaa atcgaagctg acgcggtttc cttcgatttc agtaatcgtc aggcagcagc
     5461 cgttattggc gaccgatgcg ccgtgttgca ggttttctgc cgcctcttgc ggaagctcga
     5521 cgacataagt ctgaaacgcc tccgacgggc ggtggattgc cgtcagtttt cccaatcctt
     5581 gaacaatgcc tgtaaacata atcctgtttc cctgtgtcgg taaaaatggt gcaaattgta
     5641 gcatttcccc gcgaaaaatg ccgtctgaaa ctccttcaga cggcattgtg cctccggttc
     5701 gggcaaaaac cgcccggtac gccttgacct ttcctttccg cgccggtcgg cggtcttgca
     5761 cttatccctc ctgcaaatcg gtttgcgtgt tcaagtcggc aaaatgcccg tcaaactcga
     5821 atctgaccgg ccgcgccctt tgctgctgca accagcttct taatgttttc atacccgaaa
     5881 acagataagg aatcgcgctt tgcagaattt gcggacggat atacataatg ttgtagtgca
     5941 tcgttaccgg cgtttccaca taaaacgcat tgcacaacgg tgtgcgtttg gagacgcttt
     6001 caaagcgcgc gaccaaatcg tccggcagat acggcatatc gcaaggtaca atcagcagcc
     6061 aatcggccgc cgccaactgc aaatcgttgg ctgcggtaca caatgccgaa agcgggccga
     6121 aatgctgcca ctgccgggca tccggaaaaa catgcgggct gcggcgggcg tattcttcca
     6181 aattccggtt ggcactgatg acgatatggc tgacctgcgg cctgatttta ccgataacgc
     6241 ggtcaatcag cggtctgcct tccaacagtg ccagcccttt gtcttccccg cccatacggt
     6301 cggccaggcc tcctgccaaa atcaatgcga acgtttttaa tgcgggcggg cttttgggaa
     6361 agtttgaggt tttcatagtt tcagtctgcc gtttccgata ttttagaagc gcgtttgaaa
     6421 acggcgtcgt ataaattcat gttatttgaa ttttatcata acgtgattta acactgaaag
     6481 cattgaggct taagttttcc ttaacctgtc ctttctgagc agttgtttct aattccaaag
     6541 aatgatattg tttgcaccgt ttaaaacgac tttcctccga atatgatact gccaacccga
     6601 ttttcagacg gcatccccct ttccctgcgc ctgaaactcc tgaccggctt gtgggtcggt
     6661 ttggcggcac tgtctgtcgt tttaactctg ctgctttctt tccgcctgga aaatgccgcc
     6721 tcggtcatcg aagaggcggg caatctgaag atgcaggcat accggcttgc ctatatggcg
     6781 ggcgaaggct cgccccgcgc acaaatcgac aatcagattg ccgaatttga aaaaagttta
     6841 aaacgcattt cccaaagcga tgccatccat ccgctgattc cctccgacaa tccccttgcc
     6901 tacgatttga tacagtccat gctgattatc gactggcagg ccaacatcct ccccccgttg
     6961 caggcatacc gccgccccac acaaatcgag ctttaccgct ttgccggcaa tatcgaactg
     7021 tttttgcagg cgttggaaaa tgccggcgaa aaaaacactt ggtggctcag gcggtttcaa
     7081 tgggtcatta tgctgatgac gctggtgtcg tccgtgctga tgcttttttg gcaccagatt
     7141 tgggttatcc gtccgctgca ggcgttaagg gaaggtgcgg aacgcatcgg tcagcggcat
     7201 ttcgacatcc ctgttcccga agacggtacg cccgaattca aacaggtcgg gcggtgtttc
     7261 aaccaaatgg cgctcaggct gaagacttta tacgatgatt tggaaggaca ggttgccgaa
     7321 caaacccaca accttgaaaa gcaaaaccgc aacctgaccc tgctgtaccg gaccacgcgc
     7381 gacctgcatc aatcctatac gccgcgacag gcggcggaag aatttttgaa ccacatcctg
     7441 cccgccgtcg gcgcgcaatc cggcaacatc tgcctggaaa acggttccga cacagacatt
     7501 tccgtccata cggccgaaca cggcaaaaag ccccctttgg aaaaatacca tgacgaaacc
     7561 ttccccatcg aatatcaaaa cgagaaactg ggcatgcttt cgctcggctt ttcagacggc
     7621 acttccctca ccggagacga ccgcacgctg cttcaaaccc tgattcggca attgggcgta
     7681 tcgctcgccg gggcgaaaca ggaggaagaa aaacgtctgc ttgccgtatt gcaggaacgc
     7741 agcctgattg cacaggggct gcacgacagc atcgcgcagg cattaacctt cctgaacctg
     7801 caggtacaga tgctggaaac cgcctttgcc gaaaacaaac gggaggaagc cgcagaaaac
     7861 atcggcttta tcaaaacagg cgtgcaggaa tgttatgaag atgtccgcga actgctgctc
     7921 aatttccgta ccaaaatcag caataaagaa tttcccgaag ctgttgccga cctgttcgcg
     7981 cgcttcacgc aacaaaccgg gataacggtc gaaaccgttt gggaaaacgg ttcgttcctg
     8041 cctacacagg acgagcagct ccaaatgatt ttcatcctgc aagaaagcct gtccaacatc
     8101 cgcaaacacg cccgcgccac ccatgtaaaa ttcacccttt ccgaatacgg cggacgcttt
     8161 accatgacca tccaagacaa cggacaaggt ttcgacacgg agaaaatagg agaacctacg
     8221 ggcagccatg tcggactgca catcatgcag gagcgtgcca aacgcatccg tgccgtttta
     8281 gaaatccgtt cccaagcaca acagggaacc accgtctcat tgacgggcgc acccaaagaa
     8341 agcctaccat gaccatcaaa atcatcctga tagacgacca caccctcttc cgcagcggca
     8401 tcaaagccct tttgtcgcgc caacacggtt ttgaagtcat cggcgaagcc gcagacggac
     8461 tctcgggcat caaaatgatc agtcggctgc aacccgatgt cgtcctgctc gatttggata
     8521 tgccaggcat gaacgggcgc gaggccttgt cccaaatcat cagcatcaat ccgcagcagg
     8581 cagtcattat gctgaccgtt tccgaagaca gcgacgacct taccgaatgt atgcgcatcg
     8641 gcgcgcgcgg ctacctgctg aaaaacatca acgccgattt tcttctcgaa agcatacgca
     8701 aagccgccga aggcgataat gtattctcgc ccgagatgac cgccaaactc gtcaaaagcc
     8761 tgatttcccc ccaacctgcc caaaggacgc aggcactctc ctcacttacc cctcgtgaac
     8821 tggaaatctt gggctatctc gccgcaggac acagcaacaa aatcatcgcc cgccacctcg
     8881 atcttgccga atccaccgtc aaagtccacg ttcaaaacct gctccgcaaa ctcaacctca
     8941 gcagccgggt gcaggccgcc gtttacgcca tccggcacaa cgtcccccaa cctgtgccgg
     9001 aatagacgtt cagacggcat cgggtcagga aaaaagcaat gcgccccttt gacggggtgc
     9061 aatatataag ggacggcaca ccggatgccg tccctttaag tacgccatta ccgccgtaac
     9121 ccggacggaa tggcttaggc agtttatttc caagaatatt tcacacccac ggtgccttgg
     9181 tagcctttat gtcagttgct tccgcatcct gccgataatt gccttgcctg tcccgagaaa
     9241 gggtttgatt ttcgccacgg cgttggtgga gcgtatccag catcgcatag cctgattcca
     9301 agttttgacg tggaacgagt gcgtaggccg gtgcgcttgg cgtgatgatg gttttatctt
     9361 gattcggcgt ggttaaagtc cagaagtatt ctgtcgcgcc cgcctcatct ttgtgtgaaa
     9421 ccaattgcaa ttcccccgcc cctgtggttt tggcagttcc gctaaaagta tcgcgcattg
     9481 tgtaggtatg tttagcgtct tcgatattta atttaccggt ttccgccccc ttgtcttgtc
     9541 ccaaaacctt aacaacggcg gcactgcgtt tgtagcggtc ggaaagttcg ccgatcgaac
     9601 cgtcgataat attttctttg ccatctttac ccactgaaat gaccgttgtt tttcctgaag
     9661 catcgcctgt gatttccagt aaatcagatt gagaatttcc tccttgatca tcacccggcg
     9721 agttccattc tgtattgact tttaacacac cgtctttacc gacataattt ccttcaatgg
     9781 tgagtttgtc tgcataggat tgattggcaa gcgtaatgat gccgccgttg gtgaatgttc
     9841 ctttttcaaa tacagaagtt gtacccgata aagaaatacc tgtattagat agggttagcg
     9901 tgccgcctga taataatgta gtcccttcgg gtgttgttaa tgatgtgtct ttttttaggt
     9961 gaaaagtagc ttttgttagt tttatggttc caatatcttc tgatttgaat tccattgcgc
    10021 catttgtaat ttttagcaca gcattggtaa tagggagggg gaagcgcatt gtaaatgatt
    10081 tttctgttcc atctggattg attaatgtac cagatgcgcc aaagatctct ccttcaggga
    10141 tagaaacagt tccatcagaa attgatggtt tatttgcgct tttatcactg tgtttatgaa
    10201 ttaatgtaat tggggctgta ctagattatc cctaaattcc acaccgatcc cgcaggattt
    10261 ttagctgccg ggacggtgtg ccggagttaa
//
LOCUS       DAXFZR010000079         9946 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000079 DAXFZR010000000
VERSION     DAXFZR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.080"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            52..837
                     /locus_tag="ACFJAS_001756"
     CDS             52..837
                     /locus_tag="ACFJAS_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3982935.1"
                     /translation="MRPTKNFSSLLFSSLLFSSLLFSSAAQAAGEDHGRGPYVQADLA
                     YAAERITHDYPKPTGTNKNKISTVSDYFRNIRTRSVHPRVSVGYDFGGWRIAADYARY
                     RKWNNNKYSVNTKNVRTHPDNGTREDLKTENQENGSFHAASSLGLSAIYDFQINDKFK
                     PYIGARVAYGHVKHQVHSVETGTTIVTSKNGGPIPEAPTRKPAHHESRSTRSLGFGAV
                     AGVGIDITPNLTLDAGYRYHNWGRLENTRFKTHEASLGMRYRF"
     gene            1072..1368
                     /locus_tag="ACFJAS_001757"
     CDS             1072..1368
                     /locus_tag="ACFJAS_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982936.1"
                     /translation="MQADLAYAYEHITRDYPEATGANQGKISTVSDYFKNIRTRSVHP
                     RLALGYDFGGWRVFSAPAVAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
     gene            1516..1699
                     /locus_tag="ACFJAS_001758"
                     /pseudo
     CDS             1516..1699
                     /locus_tag="ACFJAS_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776296.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
     gene            1847..2072
                     /locus_tag="ACFJAS_001759"
                     /pseudo
     CDS             1847..2072
                     /locus_tag="ACFJAS_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749744.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
     gene            2182..2859
                     /locus_tag="ACFJAS_001760"
     CDS             2182..2859
                     /locus_tag="ACFJAS_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC3982937.1"
                     /translation="MTFFKPSTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLADTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(3133..3903)
                     /gene="xth"
                     /locus_tag="ACFJAS_001761"
     CDS             complement(3133..3903)
                     /gene="xth"
                     /locus_tag="ACFJAS_001761"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC3982938.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(3963..4244)
                     /locus_tag="ACFJAS_001762"
     CDS             complement(3963..4244)
                     /locus_tag="ACFJAS_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC3982939.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            4497..4748
                     /locus_tag="ACFJAS_001763"
     CDS             4497..4748
                     /locus_tag="ACFJAS_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982940.1"
                     /translation="MDIFRESRPFCATGCPTPTSGSAFHHRRRRYGQDRIGRAFLTMH
                     RILDKENQIHTDMALALPAVVKDAVAIWADMDTLDRCSG"
     gene            complement(4811..4951)
                     /locus_tag="ACFJAS_001764"
     CDS             complement(4811..4951)
                     /locus_tag="ACFJAS_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982941.1"
                     /translation="MSGKEPLNRNPVIASGRECRLKYSSDGIFREGRDALEKTAFQAG
                     AI"
     gene            complement(4979..5860)
                     /gene="nadC"
                     /locus_tag="ACFJAS_001765"
     CDS             complement(4979..5860)
                     /gene="nadC"
                     /locus_tag="ACFJAS_001765"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC3982942.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIHDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRIGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(5936..6874)
                     /locus_tag="ACFJAS_001766"
     CDS             complement(5936..6874)
                     /locus_tag="ACFJAS_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982943.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPGFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLYRFRDNVLSDRLISDIGLPLGSR"
     gene            7076..8188
                     /gene="nadA"
                     /locus_tag="ACFJAS_001767"
     CDS             7076..8188
                     /gene="nadA"
                     /locus_tag="ACFJAS_001767"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC3982944.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKVLLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            8240..9748
                     /gene="nadB"
                     /locus_tag="ACFJAS_001768"
     CDS             8240..9748
                     /gene="nadB"
                     /locus_tag="ACFJAS_001768"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC3982945.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         2219 a         2961 c         2684 g         2082 t
ORIGIN      
        1 tattaacgat ctttttaata atttaatctt agatatttgg agttgatatg catacggccg
       61 acaaaaaact tctcttctct tctcttctct tctcttctct tctcttctct tctcttctct
      121 tccgcagcgc aggcggcggg tgaagaccat gggcgcggcc cgtatgtgca ggcggattta
      181 gcctacgccg ccgaacgcat tacccacgat tatccgaaac caaccggtac aaacaaaaac
      241 aaaataagca cggtaagcga ttatttcaga aacatccgca cccgctccgt ccacccccgg
      301 gtgtcggtcg gctacgattt cggcggctgg aggatagcgg cagattatgc ccgttacaga
      361 aaatggaaca acaataaata ttccgtcaac acaaaaaatg tgcggacaca tccagacaat
      421 ggcaccaggg aagacctgaa gacggaaaat caggaaaacg gcagcttcca cgccgcttct
      481 tctctcggct tatccgctat ttacgatttc caaataaacg ataaattcaa accctatatc
      541 ggcgcgcgcg tcgcctacgg acacgttaaa catcaggttc attcagtgga aacaggaacc
      601 acgattgtta cctctaaaaa tggaggtcct atcccagaag ctccgaccag aaaacctgcc
      661 catcacgaaa gccgcagcac ccgcagcttg ggcttcggcg cagtggcagg cgtaggcatc
      721 gacatcacgc ccaacctgac cctggacgcc gggtaccgct accacaactg gggacgcttg
      781 gaaaacaccc gcttcaaaac ccacgaagcc tcattgggca tgcgctaccg cttctgattc
      841 cccgataccg atgccgtctg aaccttcaga cggcattttt aaggcgcaag atcgggcaaa
      901 cggcattttc agacggcata actgacagta taatccgaac atccggccgc tttcgcgcgg
      961 ccttcagcat tatccgattt tgtccgaaag ccgaaccatg caatacaaac ccctcctgct
     1021 tgccctgatg cttggcaggc ggcgggtgaa gaccatgggc gcggcccgta tgtgcaggcg
     1081 gatctggctt acgcctacga gcacatcacc cgcgattatc ccgaagcaac cggtgcaaac
     1141 caaggcaaaa taagcacggt aagcgattat ttcaaaaaca tccgcacccg ctccgtccac
     1201 ccccgactcg ccctcggcta cgatttcggc ggctggcgcg tcttttccgc ccccgccgtt
     1261 gccgccggcc tccaccaatc ccttcaatat taccgatccc gccattgccg ccgattccgt
     1321 caaatccatc aattccgccg aatcacgcct attccccaaa aaccttgatg cggcgggctg
     1381 aagcccgccc tgcaaccctc tctatgcacc cccttgcgag cccgacacta cgcaacatct
     1441 tgagaaccca tcctgtcaag aatacccgaa ccgtcccgat acaccgtaat cctaaaaccc
     1501 atcattcccg cgctgcaatg ggacatcggc ggcagcgggg cggttttccc ttcgctcgca
     1561 ctgtttctgc tctgtttcat cataggtatg cacaacacgg ggatgacgct tctgccgggc
     1621 ggtgcaatcc gttcgacgca catggcccgg cacggcagcc gacttgggca tcgaaatccc
     1681 gcgcgtgccg tactatagtg gattaacaaa aaccagtacg gcgttggccc gccttagctc
     1741 aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttccgt actatctgta
     1801 ctgtctgcgg cttcgccgcc ttgtcctgat ttttgttaat ccactatatt cctaaaccca
     1861 ccatccgcgc ctgccgcacg tacacgtcga caaaccgaaa atatggctgc tcggcggcat
     1921 cgtcggcgcg tggggctgcc ataaaatcgg acaccatttc tccctgcccg tttcggcagt
     1981 attgccgata ccgggtgcag gctcggtcgg ctacgatgtg aaggtacgtt tttttgcgga
     2041 tgtggctgcg gtttcggcgc aaatcacgat aaacccgcgt tgtttcggga aaaatcgggt
     2101 aatttcaggt taattgcact ttttcagacg gcatgattgc ttatagttgt acggtttgac
     2161 atatgcacgg aaggaaacgc catgactttc ttcaaaccct ctaccgtcgt gctgaccgca
     2221 tccgcactcg cgctttccgg ctgcgttgcc gaccccgtaa ccggacagca gtccccaaac
     2281 aaatccgcca tgtacggttt gggtggcgcg gcagtgtgcg gcatcgtcgg cgcactgacc
     2341 cacagcggca aaggcgcacg caattccgcg cttgcctgcg gcgcaatcgg cgcaggcgtg
     2401 ggcggctata tggactacca agagcagcgt ttgcgccaaa accttgccga cacgcaaatc
     2461 gaaatccaac gccaaggcaa ccaaatcagg ctggtgatgc ccgaaagcgt taccttcgcc
     2521 accggcagcg cggcgttggg cggcagtgcg caatacgccc tgaacactgc cgcacagacg
     2581 ctggtgcagt atcccgacac gacgctgacc atcaacgggc acaccgacaa cacaggttcc
     2641 gatgcagtca acaatccgct ttcgcaacac cgcgcccaag cggttgccta ctatctgcag
     2701 acgcgcggcg tggcggcttc gcgcctgacg gtttacggct acggttcgca tatgccggtc
     2761 gcgtccaacg ctacggttga aggccgcgcg caaaaccgcc gcgtcgaaat cctcatcaac
     2821 cccgaccaac gcgccgtcaa cgccgcacgg cacatgtaac gcgccctgca acgccaatgc
     2881 cgtctgaatg tttttcagac ggcatttccg tattcgcaag cacagccgta ccccgactgt
     2941 aatgctatgg ataacgtctc gccgtttgcc gggaaaccgc agattgcagc cggtggcgtg
     3001 caagtgggcc caaccttgca gacgttaccg ccataccgcg atacccctgt aacctcatcg
     3061 tttaaacaga tattcaatca aaatgccgtc tgaaaccctg cgaagtttca gacggcattt
     3121 gcctttcccg aattacaaat caaattctgc cgccacgggc gcgtggtcgc tcggacgctc
     3181 cagcgcgcgc gtctccaaat cgatgcggac atccttcaaa acggccgcca tttcggatgt
     3241 aaccaatata tggtcgatac gcaggcccag tttgcgttgg aacatcgcgc cgcgatagtc
     3301 gaaccaggta tagaacgcgc cttcggggtg gacttggcgc aggctgtcgg tcaatcccaa
     3361 atccagcaga ttttgaaacc actgccgttc gacggacgaa cagtggattt tttcatacca
     3421 tttttcaggg tcgtaacagt ccgcatcggc aggcgcaata ttgaaatcgc ccagcagtac
     3481 cagtttgccg tggcgggtca tttcgtcgcg gacaaactcc gtcagtgcgg caaaccactg
     3541 ttccttatat ttgaatttgg ggctgtccaa agcctcgccg ttgacgcaat agacattgat
     3601 gacgcgcacg ccgcctacgg tcgccgcaat cacgcggcgt tgcgggtcat ccggcaggga
     3661 aggcaaaccg aaatgcacgt cctgcggcac gctgcggctg acgattgcca caccgttgta
     3721 ggttttctgc ccgctccaaa cacagtgcca gcccatcatc tgcaaggcgg cggccggaaa
     3781 tttgtcctga tcgagtttga gttcctgcaa aaccaaaata tcgggcggat tgtcggcaag
     3841 caggttttgc acctgcggca gccgcacatt gagcgaattg acgttccaag tggtgatttt
     3901 cataacattt ccgaaaaatg ccgtctgaaa cgttcagacg gcaaaagaaa aggattctaa
     3961 cactatgccg cgcgtttgtt ttccaaatcg cggattgtcc gcagaatcgt tgccgcgtct
     4021 tcggaaacca ggcggtattc gataatgcgg tggtaacgcg taaagtccac cagaccgccc
     4081 gccctcaggc ggttcaaatg acaggaaacc gcggtagccg acagggataa ggattttgcc
     4141 agttcgacga tattgcgttc gctgtccaac agttggatca gtatcgccat acgttcggga
     4201 tgcgccataa gtttgagtat ggtgtggagc ggtattgtgt tcatcggtat tcctaaaacc
     4261 ttttccgccc gccaagcgga ttgacgggcg gcagatatgg atgattttga ttatctttat
     4321 aaattgtatt ataaatcaat tgtttatttc tcattgtaca ggattgtatg ttccttttcc
     4381 cactattttt gcaaacaagc gtaaagacag gctttcagac ggcataaaac tgccgcccct
     4441 accctgcctc accctgttgc cgaacggaat catccgtatt ttttaagaaa accattatgg
     4501 atattttccg tgaatcaagg ccgttctgcg cgacaggctg tccaacgcca acatccggtt
     4561 cggcatttca tcatcgccgc cgacgatacg ggcaagaccg catcggtcgt gccttcctaa
     4621 cgatgcaccg catcctcgac aaggaaaacc agatacatac cgatatggct ttagcgttgc
     4681 cggcggtagt gaaagacgca gtggcgatat gggcagatat ggatacattg gataggtgtt
     4741 ccggctgacc gctccgttac tgccgtatct ttcggctacc gttcatcaaa tggttccaat
     4801 gccgaaatgc ctaaatcgcg cctgcctgaa atgctgtttt ttctaaagca tcacgacctt
     4861 cacgaaaaat gccgtctgaa gaatatttca gacggcattc cctccctgag gcaataaccg
     4921 gattacggtt aagcggttcc ttgcctgaca tatcagacag ccgcccgcac cctgaaactc
     4981 acgccacgaa atccaaacct atgtccaacg atcggctgct gtgggtcaga tagccgaggg
     5041 cgatgccgtc cacgccggtt tgcgccacgc gcttcaggcg gtcgaagccg atgccgcccg
     5101 atgcttcgca atagacggtg tgtgggtggg cggtttgtgt gtggcagcgg tttgccgctt
     5161 ctttcagggt ttcgtcgtcc atgttgtcca gcaaaatccg ttccgcgccc gctgcgatgg
     5221 cttcgtccag ttgtgccaat gtgtccactt cgatttccac gcaggtcaat ggtccgactg
     5281 cctgttttgc ctgccgaacg gcttgggcga tgctgccgca ataggcgagg tggttgtctt
     5341 tgatgagcac ggcgtcgtcc aaaccgatgc ggtggttcgc gccgccgccc gccctgacgg
     5401 cgtatttttg caggacgcgc agcaggggga tggttttgcg gctgcacacg atgtctgtac
     5461 cgtattcggc gacctcggca acggcacgag cggtggcggt ggcgatgccg ctcaagtgtg
     5521 tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc gcgggcgttg ccttcgacgg
     5581 cagccagtgt ctgacctgcg cggacggctt gtccgtcgtg gatttcggct tggaagcgga
     5641 cgcacggatc catcgtctga aaggcgaggc gcgccaagtc cataccggcg ataacaccgt
     5701 cttcgcggct gacgaggaaa agtttggcgg ttttgtcggg cgcgatgacg gcggcggacg
     5761 taatgtcgcc gcgcctgccc aagtcttcgc tcaaggcttg ttccaccata gggcgcaaca
     5821 aggtgtcggg cagggcaaag agggggtttt cagacggcat ggggtttcct ttgttaaaaa
     5881 agattgatgt tgcaccgatg gggcaaaact tcatttgatg ccgtctgaaa acgggctaac
     5941 ggctgcccag cggcagtccg atgtccgaaa tcagcctgtc ggacaggacg ttgtcgcgga
     6001 agcggtaaag ctgcgcggga cggcctttgc tgcccgatac gccggtatcc gacggctcga
     6061 taaggttttg ctgctgaatc tggcggcgga agttttgctt gtgcagcaat ctgccgctga
     6121 tggcttcgac gctgttttgc agttgcagca gtgtaaatcc gggcggcatc agttcgaaaa
     6181 tcacggggcg gtatttgatt ttggcgcgca ggcgggacag ggcggtcgcc aacacgcggc
     6241 ggtggtcgtg gcgcatggtc tgccccgtga gcgcgaagtc gaagtttgcc tgcggctcgg
     6301 cggcctccgc tatcagaccg ctttcataca acatttcata gcgttgcaaa acgtattctt
     6361 ccgaccagtt ttccggttcg accccccaac acaaatgaat atgcttgagc cgcttttggc
     6421 ggacttcctc cgtgtccgcc gagtttgccc aaatgcgcag gcggctgacg acggcgtcgc
     6481 gctgcccgcc gtcggtgcgc aagtcttccc atgggaaata gccgtagcag tcctgccatt
     6541 tcgcgtcggg atgcaggatg ctatcggctg cctcgcgcac cagccccaaa tagctgacgt
     6601 acagcacggg catgccgtgt tcgttgcggc ggtgggtatc gacaaaggtg taaagctgtt
     6661 ccacatagcc cataggctgc gaagtctgct tggcgaccca cagctttacg cccgcctgca
     6721 gggaattgcg caggggggaa agcgggccgt tgggcaggag cgtgccttgg gcgacggtca
     6781 acacccgcag gccgccgtcg gtaacggcaa tcaatacggg aaccagctcg acgatgcttt
     6841 gcagcggagc ttcggcttcg gggtaggcgt ccatgctcgg ctttctgcgg gtgggaaagg
     6901 ctttattgta aaccaaagcg gtttgtcgga acgaagcggg tcggttttgc ggtatcgcaa
     6961 acctgttttc agacgacctt gcatattata ctcaagttga gcataatgta aaaacagttt
     7021 gaaaaacaga cggcaaacct tcatgccgtc tgaaaaccag ctacaaggaa acatcatgca
     7081 aaccgccgcc cgccgctcgt tcgattacga tatgcccctc atccaaacgc cgacttccgc
     7141 ctgccaaatc cgtcaggcgt gggcgaaggt tgccgacacg cccgaccacg agacggcagg
     7201 tcgtctgaaa gacgaaatca aggtcttgct gaagcggaaa aacgcggtct tggtggcgca
     7261 ttattacgtc gatccgctga ttcaggattt ggctttggag acgggcggat gcgtgggcga
     7321 ttcgctggaa atggcgcgct tcggcgcgga acacgaagcc ggtacgctgg tggtggcggg
     7381 cgtgcgtttc atgggcgaga gcgcgaaaat cctctgccct gaaaaaacgg tgctgatgcc
     7441 tgatttggaa gcggaatgtt ctttggattt gggttgtccc gaagaagcgt tttcagcgtt
     7501 ttgcgaccaa caccccgacc gcacggtggc ggtgtacgcc aatacttccg cagccgtgaa
     7561 agcgcgtgcc gactgggtgg taacgtcttc ggtggcgttg gaaatcgtgt cgtatctgaa
     7621 atcgcgcggc gagaaactga tttgggggcc cgaccgccat ctcggcgact acatccgccg
     7681 cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc atcgtccata acgaattcaa
     7741 agggcaggaa ctggcggcgt tgaaggcaga gcatcccgat gctgtggtgc tggttcatcc
     7801 cgaatcgccg caaagcgtca tcgaactggg cgacgtggtc ggctcgacca gcaaactact
     7861 caaagccgcc gtatcgcgcc ctgaaaaaaa attcatcgtg gcaaccgatt tgggcatcct
     7921 gcacgaaatg caaaagcagg cgcccgacaa agaatttatc gccgcgccga cggcgggcaa
     7981 cggcggaagc tgtaaaagct gcgcgttctg cccgtggatg gcgatgaatt cgctgggcgg
     8041 catcaaacac gccctgacag gcggacgcaa cgaaatcctg ttggacagaa agctgggcga
     8101 agccgccaaa ctgcctttgc agcgtatgct cgacttcgcg gcaggactca aacgcgggga
     8161 tgtgttcaac ggcatggggc ccgcctgatt tgccgtccat atcgccgttt cagacgacct
     8221 ctcaaacaag gacaacacca tgcaaaccga ttgcgacgta ttgattgccg gaaacgggct
     8281 ggcggcactg acgctcgccc tgtcgctgcc tgaatcgttc cgcatcgtca ttttgtgcaa
     8341 aaaccggctg gacgacaccg ccagccgtca tgcgcaaggc gggattgcgg cggcgtggtc
     8401 gggagaggac gacatcggaa aacacgtcgc cgatactttg gaagcgggcg caggtttgtg
     8461 cgacgaagcc gccgtccgca ccatcctgtc gcagggcaaa ccggcaatcg aatggctgct
     8521 ggcgcagggc gtggcgttcg accggaatca taacgacctg cacctgacgc gcgaaggcgg
     8581 gcatacctgc cgccgaatcg cccacgtcgc cgactacacg ggtgaagccg tcatgcagag
     8641 cctgattatc caaatacgcc gccgaccgaa catccgcgtt tacgagcggc agatggcgtt
     8701 ggacgttcaa accgaatcag gcgcagcgtg cggactgacc gtcctcgact gccgaacgca
     8761 agaaacctac cgcatccgtg cccgccatac cgtactcgca ggcggcggcc tcgggcaaat
     8821 ttacgccgcc accaccacgc cgcccgaatg cacgggcgac gccatcgcca tggcgatacg
     8881 cgcgggttgc gcaatcgaaa acctcgaatt tatccaattc caccccacag gcttggcaag
     8941 gccgtctgaa aacggccgca ccttcctgat ttccgaagcc gtgcgcggcg aaggcggcat
     9001 cctgaccaat caatcgggcg aacggtttat gccgcattac gaccgccgcg ccgaactcgc
     9061 gccgcgcgac atcgttgccc gcgccatcgc cgccgaaacc gccaaacaaa cgcaagactt
     9121 cgtctcgctc gacatcagcc gccaacccgc agcgttcgtc cgtcggcatt tcccgtccat
     9181 ccatcggcac tgcctgtccc aatgcggctt ggacatcacg cgccaagcca tccccgtccg
     9241 ccccgtgcaa cactacacct gcggcggcat ccaaaccgac cccagcggca gaacctccct
     9301 gccgcagctc tacgccttag gcgaaaccgc ctgtaccgga ctgcacggag ccaaccgcct
     9361 cgccagcaac tccctgctcg aatgcgtcgt taccgccagg cttgccgccc aaggcatcgc
     9421 ggacggacaa gcgttccaaa tcgaaccatt ccaaaggccg tctgaaagcc cctccgccga
     9481 aacagacatc ttttcaaacg acctccaaaa cacattcagc cgccctgtcc tgcaaacgtt
     9541 caaccaacgc catctcggca tcctccgcaa cgataccgac ctgcaccgcg ccatcgacca
     9601 actgcggctt tggaagcaaa accaagccga accgcacacc gcgtccgaat acgaaaaccg
     9661 caacctactc gaatgcagcc tcgccgtcgc ccaagccgca tacaggcgga gacagaacat
     9721 cggtgcgcat tttaatagtg attgttaagg ttggctggtt agaaaaaaat cgttcaaata
     9781 aatttgaata gcatggttaa gagattggca ttcagttttt atggttgcgt aagttgggtt
     9841 gaggcctttg caaaaaagcc cttccttcga catccgaaac ccaaacacag gttttcggct
     9901 gttttcgccc caaatacctc ctaattttac ccgaatatcc ccttaa
//
LOCUS       DAXFZR010000080         9820 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000080 DAXFZR010000000
VERSION     DAXFZR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.081"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..440)
                     /gene="cca"
                     /locus_tag="ACFJAS_001769"
     CDS             complement(<1..440)
                     /gene="cca"
                     /locus_tag="ACFJAS_001769"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360171.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HFC3982946.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYG"
     gene            complement(519..2147)
                     /locus_tag="ACFJAS_001770"
     CDS             complement(519..2147)
                     /locus_tag="ACFJAS_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC3982947.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            2297..2671
                     /locus_tag="ACFJAS_001771"
     CDS             2297..2671
                     /locus_tag="ACFJAS_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC3982948.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            2720..4258
                     /locus_tag="ACFJAS_001772"
     CDS             2720..4258
                     /locus_tag="ACFJAS_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC3982949.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            4325..4942
                     /gene="recR"
                     /locus_tag="ACFJAS_001773"
     CDS             4325..4942
                     /gene="recR"
                     /locus_tag="ACFJAS_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC3982950.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(4998..5276)
                     /locus_tag="ACFJAS_001774"
     CDS             complement(4998..5276)
                     /locus_tag="ACFJAS_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982951.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            5502..6749
                     /locus_tag="ACFJAS_001775"
     CDS             5502..6749
                     /locus_tag="ACFJAS_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC3982952.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            6742..7437
                     /gene="lolD"
                     /locus_tag="ACFJAS_001776"
     CDS             6742..7437
                     /gene="lolD"
                     /locus_tag="ACFJAS_001776"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC3982953.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            7504..9249
                     /gene="recD"
                     /locus_tag="ACFJAS_001777"
     CDS             7504..9249
                     /gene="recD"
                     /locus_tag="ACFJAS_001777"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC3982954.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALDSMLERVFSQE"
     gene            complement(9321..9820)
                     /locus_tag="ACFJAS_001778"
                     /pseudo
     CDS             complement(9321..9820)
                     /locus_tag="ACFJAS_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
BASE COUNT         2274 a         2547 c         2803 g         2196 t
ORIGIN      
        1 aagccgtaac gcgcggcaaa gcgggcggca cgaaggatgc ggacgggatc ttcggcgaag
       61 gcgggtgaga cgtggcgcaa aatgcctgcc gccaaatccc gttgcccgcc gaaagggtcg
      121 ataatcttgc cgtccgcatc ttgcgccatc gcgttgatgg tcaggtcgcg gcgcatcaag
      181 tcctgttcca gcgtaacgtc tttgtcggcg tggaaactga aaccggcgta acccttggcg
      241 gttttgcgtt cggtgcgggc tagggcgtat tcttcgtgcg ttttgggatg gaggaataca
      301 ggaaaatctt tgccgaccgg ctggaaacct tgcgccaaca tggtttgcgc gtctgcgccg
      361 acgaccaccc aatcgcggtc tttgacgggc aggcccaaaa gataatcgcg gacggcgccg
      421 ccgacgaggt aagtctgcat atgcttccta ttttaaagtt atcaacaatg ccgtctgaag
      481 cggcttcaga cggcattgtt tcaaccggag gggtgcgctt atgctacgcc ttttttctcg
      541 agatagcttt cgtaatcgcc caagtagtgt tcatatccgc ctttgccgtc cagttcgatg
      601 atttgggttg ccagggagga aacgaactga cggtcgtggg agacgaaaat cagcgtgccg
      661 ttgtatttgt ccagtgccat gttcagcgat tcgatgcttt ccatgtccat atggttggtc
      721 ggttcgtcca tgaccaagac attgggtttc aacagcaaca gtttgccgta aagcatacgg
      781 cctttttcgc cgccggagag aactttcact ttttttacca cgtcgttgct gccgaaaagc
      841 aaacgcccca aagtgcctcg gatgacttgt tcgtcgtcgc cttcccgacc ccattggcgc
      901 atccattcgc tcaagtccat atcgacgtca aagtcgtttt catggtcttg cggatagtag
      961 ccgacactgg ctttttccgc ccatttgatg ctgccttcgt ccggcaacag gccgtctgaa
     1021 tattcggggt tgtacgcgcc ggccaagagt ttcagcaagg tagatttacc tgcgccgttc
     1081 gggccgatga tggcgaggcg ttggcctgct tcaaggatga agttgaggtt tctaaacaac
     1141 tgggtttcaa agcgtttggc cagtttttca acttccacag cctgacggtg cagcttggct
     1201 ttttcatcgg cttcaaaacg gatatacggg ttttgacggg tagaaggttt gacttcgacc
     1261 atttccgatt tgattttgtc ggcctgtttc agacggctgg ttgcctgacg ggctttggat
     1321 ttattggcgg aaaaacgggc aacgaattct tgcagctctt gcagtttctc tttggctttg
     1381 gcattgtctt tcagggcgcg ctcacgcgat tgggcggaag cgagcatata gtcgtcgtag
     1441 ttgcccggat aaatagtgat ggtgttgtag tccaaatccg ccatgtgggt gcaaacttcg
     1501 ttcaagaagt ggcggtcgtg cgagatgatg atcatggtgg agtcgtattg gttcaacacg
     1561 ccttccagcc agcggatggt attgatgtcc aagttgttgg tcggttcgtc caagagcaat
     1621 acatccggct tggagaacag cgcctgcgcc agcaatacgc gcagtttgaa gcccggggcg
     1681 acttccgcca ttttcgcatt gtgcaaatct tcggaaatgc ccacgccgct taataattcg
     1741 gcggcgcgcg cttcggcggt gtagccgtcg tattcggcga acttggcttc cagttcggcg
     1801 gctttcatgt agtcgtcttc ggtggcttcg ggattggcgt aaatcgcatc gcgctcggtc
     1861 atcgccgccc acatttcggt atgccccatc atcaccacgt ccagcacgcg catgtcttcg
     1921 taggcaaact ggtcttggcg caatttaccc aaacgcacgc cgttttcaat cgccacttcg
     1981 ccggcggtct gttccaaatc gccgccgagg attttcatga aggtggattt gcccgaaccg
     2041 ttcgcgccga tcaagccgta gcggttgcct tcgccgaatt ttacggatac gttttcaaac
     2101 agcggcttcg cgccgaactg catggtaatg ccgttggtag aaatcattat gggtaaaacc
     2161 tttattgaac aagagattaa atttttcgaa ttttcggatt gtaacataac cgccccgccg
     2221 tctgaaacag acgggcaaat tttcagacac ttatagtaaa atatcggttt tattatttca
     2281 ggcaaaaggt ttccaaatga taatccttca cggcattcca aattgcgaca cagtcaaaaa
     2341 agccaaaaac aggcttgccg gatacggctt ggagtttggg tttcgggatt tcaaaaaaca
     2401 gatgccgtct gaagcggaaa tctgctcgtg gctggaacaa gtgcctttgg caaccctgtt
     2461 caacaaacgc gggacaagct ggcgcaaact cgatgccgaa acacagcaaa aagccctgtc
     2521 ctcgacggcg gaggccgtca aactgatgtc cgaaatgccg agcctgatca agcgtcccgt
     2581 attggagtgc ggcagcaggg tttacgtcgg cttcagcgaa gaaacctacg acggcatctt
     2641 caaccggcaa gccccgtgca gacagggata aaaaccgtta caatacccga cttgaatttc
     2701 ccattcccaa tctgaaaata tgttccattc catcgaaaaa tacagaacac ccgcccaagt
     2761 cttattaggc ctgattgcat taacttttgt cggcttcggc gtcagcacgg tttcccatcc
     2821 gggcgccgac tacatcgtcc aagtgggcga cgaaaaaatc agcgagcact caatcaacaa
     2881 cgccatgcag aacgagcagg cggacggcgg cagcccttgg cgcgacgcgg tgttccaatc
     2941 cctgctgcaa cgcgcctacc tgaaacaggg cgcgaagctg atgggcattt cggtttcttc
     3001 cgaacaaatc aagcagatga ttgtggacga tcccaatttc cacgacgcaa acggcaaatt
     3061 cagtcacgcg cttttgagtc aatacctgtc gcaacgccat atgtctgaag accagtttgt
     3121 cgaagaaatc cgcgatcagt ttgccttgca gaatttggta agcctcgtcc aaaacggcgt
     3181 attggtcggc gacgcgcagg cggaacagct gatcaggctg acgcaggtca accgcaccat
     3241 ccgttcgcac actttcaacc ccgacgagtt catcgcccaa gtcaaagcgt ctgaagccga
     3301 tttgcagaaa ttttataatg cgaacaaaaa agactatctg ctgccgcagg cggtcaaatt
     3361 ggaatatgtc gccttgaatc tgaaggattt tgcagacaag cagaccgtca gtgaaacgga
     3421 agtgaaaaat gcgtttgaag agcgcgtggc gcgtttgccg gcacatgaag ccaaaccttc
     3481 tttcgagcag gaaaaagccg ccgtcgaaaa cgaattgaaa atgaaaaagg cggttgccga
     3541 cttcaacaag gcaaaagaaa agctgggcga cgatgcgttc aatcatccct cctcgcttgc
     3601 cgaagccgcc aaaaacagcg gtttgaaagt ggaaacccaa gaaacttggc tgagcaggca
     3661 ggacgcacaa atgtccggca tgcccgaaaa cctaatcaat gccgtattca gcgacgacgt
     3721 attgaagaaa aaacacaatt ccgaagtgct gaccatcaac agcgaaaccg cgtgggtcgt
     3781 ccgcgccaaa gaagtccgcg aagaaaaaaa cctactgttt gaagaagcca aagatgcggt
     3841 gcgtcaggcc tatatccgta ccgaagccgc caaacttgcc gaaaacaagg caaaagaagt
     3901 gcttacccaa ctgaacggcg gcaaggcagt tgacgtgaaa tggtcggaag tgtccgtttt
     3961 gggcgcgcag caggcaaggc agtccatgcc gcccgaggct tatgcggaac tgctgaaagc
     4021 aaaaccggca aacggcaaac ccgcctatgt cagactgacc ggtctgccgg cacccgtgat
     4081 tgtcgaggtg caggcagtca cgcctccgga ggatattgcc gcacagcttc ctcctgcgaa
     4141 acaggctttg gcgcaacagc agtctgccaa tactttcgac ctgctgatcc gctatttcaa
     4201 cggaaaaatc aaacagacta aaggagcaca atcggttgac aacggcgatg gtcagtaatt
     4261 gacacttttg ttgacaacat aacggtcgga atattccgac cgttttccca tatggcggag
     4321 tatgatgaat tccaaaaaac aggatgcatt ccaacgcctg atcggtgcgc tgaaagtatt
     4381 gcccaacgtc gggccgaaat cggcacagcg gatggcgtac catctgttgc agcaaaagcg
     4441 caaagaggct gaagagctgg tggatgcctt acagacggca ttgaggcagg ttcgccattg
     4501 cgcaaggtgc aatacatttt gcgaaggcgg attgtgcgat atttgtgccg atgaaacacg
     4561 cgacgggcgg cggctgatgg tggtgcatat gcctgccgac gtgtcgaata tagaagcggc
     4621 aaactgccac gacgggctgt atttcgtcct gatggggcaa atcaatacgg cattgggaat
     4681 ggacgtatcc gccatcgcat tggacaggct ggcgcaacgg ctggacggcg gggaaatcga
     4741 agaaatcatt atcgcgaccg cctttaccgc agaaggcaat gccacagcgt atgtcctgtc
     4801 cgagtttttc aaaaacctgc cttacaaggt cagcaggctg tcgcagggca tcccattggg
     4861 cggcgaattg gaatatgtcg atgcgggaac gctggcgcag gcggtgtacg aacggcgtct
     4921 gattaaagaa ggcggggcgt aacgccgcca atgcaaaatg ccgtctgaag ccttcagacg
     4981 gcattttttc gggcggttta cttgcagttg agcccttgaa tctgcaaagt gcccccgctg
     5041 ccgaccgtaa agacgcactg cgccgcgctg ctgccggaaa cggtaatgct gccgctgccg
     5101 ccgttcaggc tgacaggttc cttaacgccc ataccgactg ccgttgccgc cagcttttgc
     5161 gcgtcggctt cggaataagg cgtgttgttg gcatcgattt tgatttcttt ggcataggct
     5221 gtcgaaacag taaaaacggc aacagcacac aaagcggaga ttttcctgat attcattgtt
     5281 ttccttaaag aaaaggtttt gtttcaagag aaggataggg tagttcggcg cgttgtgcaa
     5341 gttttccgtc ctgtttttcc gaatttgcca cataccgcgt ctttatttac acagcctgcc
     5401 gttcggacgc tgccggggtt ttacggtaca ataaactttt gccgcctccc ccaaaccgcc
     5461 gtccggggcg gcacgtttgc agacattttt aaggtagcgt tatgttttct ctagaggctt
     5521 ggataggctt gaggtatctc agggcgaaaa agcgcaacgg ctttatgtcg tttatcacga
     5581 tggtttcgat tgccggaatc gccttgggcg taaccgcgct gattgtcgtc ttgtcggtta
     5641 tgaacggctt tcagaaagaa atacgcgggc agctcctgaa tgtcgcgccg cacgccgaaa
     5701 tcggctatat cgacaatacg gatacggatt ggcgcaacct gcttcggttt gccgaaaacc
     5761 gcaaaggcat tttggctgcc gcaccctatg tttccaatca ggcattgctg gcaaacgcgg
     5821 gcgaaatcag gggcgttcag atacgcggca ttttgccgtc tgaagagcgc aaagtggtgg
     5881 aatacggcga caaaatgccg gcaggcaagt ttgaagacct gattccgggc gagtttgaca
     5941 ttatcctcgg cgtcggtttg gcggaggctt tgggggcgga agtcggcggc aaggttacgg
     6001 taattacgcc ggagggcaac gttacccctg ccggagtcgt gccgcgcctg aagcagttta
     6061 ccgtggtcgg cctggtcaag acgggtgttt acgaagtgga caactcattg gcgatgacgc
     6121 atatccaaga cgcgcgcgta ctgtaccgct tggataagga agttgcgggg ctgcggctga
     6181 agcttgccga tccgcaaaac gcccccgcct tgacggcaac gctgattccg gaggcgcaaa
     6241 gggacacggt ttgggtgcgc gattggacgt acagcaaccg cagctatttt gaagcggtcg
     6301 aactggaaaa acggatgatg ttcattatcc tgacgctgat tatcgccgtg gcggcgttca
     6361 accttgtctc ttccttggtg atggcggtta cggaaaagca ggcggacatt gcgattttgc
     6421 ggactttggg tctttcccct gccggcgtga tgaagatttt tatggtgcag ggcgcgtttt
     6481 caggcttttt cggcacgctg gcgggtgtgg tctgcggcgt gctttggggt tggaacgtcg
     6541 gcagggtcgt ggcgtttttt gaaaacctgc tcggtgtcca cctcatcaat tcgcaggttt
     6601 attttatcga ctacctgccc agtgatgtcg atatgggcga cgttgccctg attgcctgca
     6661 tttccttggg gctgtctttc gtggcgacac tgtacccgag ctggcgcgcg tcaaaaaccc
     6721 aaccggcgga ggctttgcgt tatgagtgat ttgattttga aatgcgaagg cgtgggcaaa
     6781 cgctaccgtg acggcggttt ggacgttccg gtgctgtacg gcttggattt ggaaatccgc
     6841 acgggggaaa gcaccggcat catcggctct tcgggcagcg gcaaatcgac gctgctgcat
     6901 attttgggcg ggctggatat gccgtctgaa ggcagggtgc tgctgatggg cgaggatttg
     6961 cgtgccttaa accagcggcg tttgggcgat ttgcgcaacc gccatctcgg tttcgtgtac
     7021 cagttccatc atcttctgcc tgaattttcg gcactggaaa acgtgatgat gccgcttctg
     7081 atcggcaaaa aaagccgtaa agaggcggta gaaacggcaa tggcgatgct cgacaaggtc
     7141 ggactgaaac accgttcgac gcaccgtgcg ggcgaattgt ccggcggcga acgccagcgt
     7201 gccgccattg cgcgcgcctt ggttacgcaa cccaaatgcc tgcttgccga cgaaccgacc
     7261 ggtaacctcg accgggcaaa cgcaaggaat gttttggata tgatgctgga actgaaaacg
     7321 gaattgggaa cggggctggt ggtcgtaacg cacgacgacg aactcgccgg ccgcttcgag
     7381 cgcgtgatgg tcatgaaaga cggcagcctg caccccagac agggcgcaaa cgcctaaaca
     7441 gccgaaacaa cacgcaccgt ctgaagcccc gtgccttcag acggcatttc gacaaggaat
     7501 acgatggaac tgcagacaga tgaatttgcc caagcggcgg caagggcggc aatccgtttt
     7561 ttggaacgct atgcgggttc gggcaacgaa gtgcttgcaa actgtaccga acgcctgttt
     7621 caggcattgc aaaacggtca ttcgtttatc cgtttgagcg gggacgaggc tgacgcgctg
     7681 tcggcactcg cacccgtggt cggaacatcc gccgcgcctt tgattttgga aggcagaagg
     7741 ctgtttttgg gcaggatgtg gcagttggaa tacgatttgg ctgccgaaat aaaacgcttg
     7801 gcggcagccg gcacgtccgc ccccgacgcg gcaggcgcgc ggcaaaacct cgcaaaatgg
     7861 tttcaaggcg cgggaagcga aggacagcgc gatgcggccg ccttggcact gttgcagttt
     7921 tttatggtga ttaccggagg gccgggaacg ggcaaaacga caacggttgc caaactgctg
     7981 gcactgattt gcggtgaaaa cgaaaatctt ccccatatcg cgcttgccgc accgacgggc
     8041 aaagcggcgg cacatatggc gcgcgcactg caccgtgcaa tcaacggttt tgacgcgccg
     8101 gaggccgtcc gccgccattt gctcaaactg gaagggcaaa ccgtccaccg actgcttaag
     8161 ctgagcccgc ccaaaatgca ggcggcgttc gaccatatcc gtccgctgcc gttcgacgtg
     8221 ctgattgtcg atgaagcctc catgttggat accgcattga tgctgcaact tttgaaagcg
     8281 gtcaaaaccg gcgcgcgcgt gattctgctg ggcgatgaaa accagctccc gtccgtcgga
     8341 atcggggcgg tgctgtccgt tttgtcgcaa aaaaccgttt tggacggaga aacgcaccaa
     8401 aggctggccg gtttccttcc ggaacacggt ttcagcgtca gcgcaaatcc gcccgtgttg
     8461 gcgcaaaaca ccgcccatct gtcgttcagc caccgcttcg gcgacaacag cggcatcggc
     8521 tgccttgccc gtgccgccgt atcgggcgat gaaggggcgt gggcattgtt tgaccggttt
     8581 ccggacgaac tggaacattc ggaatgcagt ccgaatgctc gggtcgaaag gttgtaccgg
     8641 gcgcacaaag cctattggca ggcggtaaaa gacggcaata tcgaagccgc atacgcaggt
     8701 atctcggata tcgtggttct ggcagcttgg cggcaggatg cggaagactt caacgaagcc
     8761 tattgcagct atgtacgccg caaaatgaac ataccggaac atttggccta tttcgccgga
     8821 gagccgatta tgatcagaca gaacgactac gcgcttgagc tgttcaacgg cgacatcgga
     8881 ctgattatgg aagatgtcgg acggcagggc agccttgccg cctattttgc cgatgcggac
     8941 ggatttaaaa aggtagcggt aagctgcctg cccgaatttg aacccgcatt cgccatgacc
     9001 gtccacaaaa gccaaggttc ggaatatcgg gaagtctggc tgctgccgcc ttccgacgca
     9061 ccttcggacg aaggggacga tgcattgtcc ggattgagta aggagctgtt atataccgcc
     9121 attacccgcg ccagggaaaa attcgtattt ttcggcggca aaaagacatt ttgtcaggcg
     9181 gtaaacaccg tcaaagtccg tcagacggcg ttggacagta tgcttgagcg ggtattttca
     9241 caagaataat ccgcccgaat gatgccgtgc cggcggcgcg ggcgttccga tgcctaccgt
     9301 cggaagcacg ccaccgcccc ttatgccttt ttcaaacggc acaggaaagt agtttccctg
     9361 tttcgggcaa aagccagcgg atcgttcgaa tcggcagctt ttttgtgaac cggtttggtt
     9421 ttcaaaacat cctcaacacg caaacccgcc cggataaatg ccttttccaa aaagtcggca
     9481 gaccgcagcc ccaaatgcac ggcgaggtcg gaaatgataa gacggatttc tccgtcggga
     9541 ttcagatgtt tggcgcatca cgcaaaaacg cagccagcat cgcagattcg gggtcgtata
     9601 acgcggattc gacggcggaa gtcggcttgg cgggaagccg gggcggattg cagacaatca
     9661 ggtcggcaaa cccttcggga aataaatcgg tttcgcggat ttcaacctgt ttttcaaaac
     9721 ccaaacgggc aatattggca cgggcgcagg caaccgccct cggattggta tccgtgccga
     9781 tgacggaagg aatgccctgt ttcgccaaaa tggcggcaag
//
LOCUS       DAXFZR010000081         9784 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000081 DAXFZR010000000
VERSION     DAXFZR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.082"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            14..604
                     /locus_tag="ACFJAS_001779"
     CDS             14..604
                     /locus_tag="ACFJAS_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC3982955.1"
                     /translation="MLGLCLLTPASYQNIHVRSWKARRDSGIVKQDLDFSCGAASIAT
                     LLNNFYGRHYSEAEILDKMDKTQMRTSFDDMQRIMPELGFEAQGYALPFEQLVQLKIP
                     VIVYLKYRKNNHFSVLNGINGETVLLADPSLGHVSTSKSQFLSAWKTRDGEMEGKILA
                     IVPKNTDFVRNQMFFNKNPVRQTRFTVEQIQMRQKR"
     gene            complement(880..1431)
                     /locus_tag="ACFJAS_001780"
     CDS             complement(880..1431)
                     /locus_tag="ACFJAS_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982956.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNPRYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAGKADG"
     gene            1669..3141
                     /gene="pyk"
                     /locus_tag="ACFJAS_001781"
     CDS             1669..3141
                     /gene="pyk"
                     /locus_tag="ACFJAS_001781"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC3982957.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            3375..4727
                     /locus_tag="ACFJAS_001782"
                     /pseudo
     CDS             3375..4727
                     /locus_tag="ACFJAS_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
     gene            complement(4810..5232)
                     /locus_tag="ACFJAS_001783"
     CDS             complement(4810..5232)
                     /locus_tag="ACFJAS_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982958.1"
                     /translation="MAGCLTDLENCLEKYLEQFGPVSESIEACTAKLQEQPSFFNRLM
                     KANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHNRKADYAEKGKERIAL
                     FPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(5415..5564)
                     /locus_tag="ACFJAS_001784"
     CDS             complement(5415..5564)
                     /locus_tag="ACFJAS_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982959.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(5582..5779)
                     /locus_tag="ACFJAS_001785"
     CDS             complement(5582..5779)
                     /locus_tag="ACFJAS_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982960.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(5789..6802)
                     /locus_tag="ACFJAS_001786"
     CDS             complement(5789..6802)
                     /locus_tag="ACFJAS_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982961.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFDNTVG
                     MAAQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNAA
                     GGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGGL
                     DTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRGY
                     SID"
     gene            complement(6901..8115)
                     /gene="gltS"
                     /locus_tag="ACFJAS_001787"
     CDS             complement(6901..8115)
                     /gene="gltS"
                     /locus_tag="ACFJAS_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC3982962.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(8465..8661)
                     /locus_tag="ACFJAS_001788"
                     /pseudo
     CDS             complement(8465..8661)
                     /locus_tag="ACFJAS_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8668..>9784)
                     /locus_tag="ACFJAS_001789"
     CDS             complement(8668..>9784)
                     /locus_tag="ACFJAS_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3982963.1"
                     /translation="NQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQDQP
                     YSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTPLSR
                     DYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNKKEI
                     AAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLKRVG
                     DDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLAYPV
                     LIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSDDVW
                     RRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
BASE COUNT         2502 a         2730 c         2333 g         2219 t
ORIGIN      
        1 ccaaatatag tttttgcttg gcctatgttt gttgaccccc gcttcttacc aaaacatcca
       61 tgtacggagt tggaaagcac ggcgagattc cggcatagta aaacaagatt tggatttttc
      121 ttgcggcgcg gcttcgattg ccacgttact gaataatttt tatggcagac attattctga
      181 agcggaaatc ttagacaaaa tggataaaac ccaaatgcgt acttcttttg atgatatgca
      241 acgcataatg cccgaactgg gttttgaagc acaaggttat gctttgccat ttgaacagtt
      301 ggtacaacta aaaattcctg taattgtgta tttaaaatac cgtaaaaaca accatttttc
      361 ggtattgaac ggtattaacg gagaaactgt tttgctggct gatccatcgc tggggcacgt
      421 ctcaacgagc aaatcacaat tcttgagcgc atggaaaaca cgtgatggcg aaatggaagg
      481 aaaaatttta gccatcgtgc caaaaaatac tgattttgtt agaaatcaga tgttttttaa
      541 taaaaatccc gttcgtcaaa cacgttttac ggtagaacaa atccaaatgc ggcaaaaacg
      601 ataataaacc gatttttgcc catttatttt atatagtgga ttaacaaaaa tcaggacaag
      661 gcggcgggcc gcagacggta caaatggtac ggaaccggtt cgcccggtgc ttgggcgcct
      721 tagggaatcg ttccctttgg gccggggcgg ggcaacgccg taccggtttt tgttaatccg
      781 ctataaaagc aaagaaaccg ccattagcac tacacccccc caaactgcgg ctgccaaaca
      841 catcggctgc ttctcgacgc acccgctgaa aaatgcgccc tatccgtccg ccttgcccgc
      901 accccctgcc tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc
      961 gatgacgcac gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcggatt
     1021 ccccgtattg accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca
     1081 cagcgaaggc gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat
     1141 ttccaagacc tgccgccgcc gcaccgaacg gttgagcaga aacacaaccg ccaacccgaa
     1201 acagaccacc gccgccatac cgccgttggc gacaatctgc aaactgttga acgcaatatc
     1261 gggcgtaacc gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaacccctg
     1321 caacacattc aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc
     1381 actcaaaacc cgccacatct tcctgtccga ccaaccgttc aaaaccttca ttttacccga
     1441 ccgcgcaagc cgccgaacaa aaaataaatt tagcctaaat cataaaaccg tgtaattttg
     1501 caccaaaaaa ggcggtttgc aggtaaaata tcaaaccatt aatttaaatc atatttttac
     1561 agaatattcc gccgcgttca ccaaatggac atcaaaccgg ttccaaattt ctgtaatttt
     1621 gcaacaaaat accgcaaaac acccgattga gaccgaaagg actttcatat gaaccagaca
     1681 agccgcgatc tgacccgcat cagccacaac accaaaatcg tcgccaccct tgggccgggc
     1741 agcaacaacg tcgaactgtt ggaagacatg atccgcgtcg gcggcctgaa cgtcgtccgc
     1801 ttcaacttca gccacggcac gcccgaattc catcaggaaa acgcccgcat cgtgcgcgag
     1861 gcggcaaaac gcgccggaca ggaaatcgcc atcattgccg atctgcaggg cccgaaaatc
     1921 cgcgtgggca aaatcgccgg cggcggcatc gaattgaaca aaggcgaaac attggtactc
     1981 gatgccgcgc tcgaaggcga aggcacgcgc gaggcggtcg gtttggacta ccgcgacctg
     2041 cccgacgacg ttgccgcagg cgatgtcttg tggctggacg acggcctgct gaccctgacc
     2101 gtggaatccg tcgaaggcag caggattatc acaagggtgg aaaacagcca catcctgaaa
     2161 agcaacaaag gcatcaacaa acgcggcggc ggtttgtccg caggcgcgtt gaccgaaaaa
     2221 gacttccgcg acctgaaaac cgcgattgcc atcggttgcg actatctcgc catcagcttt
     2281 gtgaaatccg ccgaagacct gcacatcgcg cgcgccaaag tcgaagagga aatgaagggc
     2341 agcactgccg tccgccccgg tttggtttcc aaaatcgaac gcgtagaagc gattgaaaac
     2401 cttgatgaaa tcatccttgc cggcgacggc attatggttg cgcgcggcga cttggcggtc
     2461 gaagtcggac acgccgccgt ccccgccctg caaaaacgga tgatccgccg cgcccgcgag
     2521 ttgcgccgct tcagcattac ggcgacgcaa atgatggaat cgatgattac caaccccgtg
     2581 ccgacccgcg cggaagtcag cgatgtggca aacgcggtat tggacggcac cgatgcggtg
     2641 atgtgttccg ccgaaaccgc cgtcggcgcg tatccgtttg aaaccgtcag ccaaatggcg
     2701 attatctgcg cggctgcgga aaaagaacag gattcgctca acggcgttgc cgaacagacg
     2761 gagtatcccg aagccgtcag caccaactta gcgattgccg gcggcgcggt cagcgtggcg
     2821 cgcgcggttc acgccaaagc catcgtcgcc ctgaccgaaa gcggttcgac cgcctttgaa
     2881 atcagccgcc acaatattac cctgccgatt ttcgcgctga ccccgagcgt ttccgcccaa
     2941 cgccgtatgg cgatgtaccg gggcgtgcgc ccgctgattt tggcaaccag caccgaccac
     3001 gacacggcgt tgaacgaagt cgaaacgatg ctggtggaac acaacatcct gcattccggc
     3061 gaccagtaca tcatcaccag cggctcgcaa atgcgcgaat ccggttcgac caatacgctg
     3121 gaagtgctgc gcgtcaaata atccgccctg agtggaaaat gccgtctaaa gccgatactg
     3181 tcgggcttca gacggcattt tatttgcatt ccgtcaggta aaaccgaaag aattgtgcaa
     3241 atccggcaac atcggataaa atcgagtgcc tatactaaag cgaaacaagg catttccgac
     3301 tgcctttttt atttgtccac cgtccgcctt tttacggaaa ccgaaatgac ccctttcaca
     3361 ctgaaaaaac cgtcctgctg ctcggcactg cctttgccgc cgcatctgtc cacgcatccg
     3421 gctaccactt cggcacacag tcggtcaacg cgcaaagcac ggcaaatgcc gccgacgcgt
     3481 cgaccatctt ctacaatccc gccggcctga ccaaactcga cagcagccag atttccgtca
     3541 acgccaacat cgtgctgccc agcattcatt atgaagcaga ttccgccacc gactttaccg
     3601 ggcttcccgt ccaaggttct aaaaacggca aaatcaccaa aaccacggtc gcaccccaca
     3661 tttacggcgc atacaaagtc aacgacaatc tgaccgtggg cttgggcgtg tacgtcccct
     3721 tcggctctgc caccgaatac gaaaaagatt ccgtgttgcg ccacaacatc aacaaactcg
     3781 gtctgaccag catcgccgtc gaacctgtcg ccgcgtggaa actcaacgaa cgccattcct
     3841 tcggcgcagg catcatcgcc caacataatt ccgccgaact gcgcaaatat gccgactgag
     3901 gaatcccaaa aaaagcgcaa atgctgcaag caacaccttc taatcctact gccgctgctc
     3961 aaatcaaggc cgacggacac gccgatgtca aaggcagcga ttggggcgtc ggctaccaac
     4021 tggcgtggat gtgggacatc aacgaccgcg cgcgcgtggg cgtgaactac cgttccaaag
     4081 tttcacacac gctcaaaggc gatgccgaat gggcggcaga cggcgcggcg gcgaaacaac
     4141 agtggaatga caatatgctc acaccgctcg gttacacggc gaatgaaaaa gcccgcgtca
     4201 aaatcgtaac gcctgagtct ttgtccgtac acggcatgta caaagtgtcc gacaaagccg
     4261 acctgttcgg cgacgtaact tggacgcgcc acagccgctt caataaggcg gaactgtttt
     4321 ttgaaaaaga aaaaaatatt gctaatggca aaaaatccga ccgcaccacc atcaccccca
     4381 actggcgcaa cacctacaaa gtcggcttgg gcggttctta tcaaatcagc gaaccgctgc
     4441 aactgcgcgt cggcatcgct tttgacaaac cgcctgtccg caacgccgac taccgcatga
     4501 acagcctgcc cgacggcaac cgcatctggt tctccgccgg catgaaatac catatcggca
     4561 aaaaccacgt cgtcgatgcc gcctacaccc acatccacat caacgacacc agctaccgca
     4621 cggcgaaggc aagcggcaac gatgtggaca gcaaaggtgc gtcttgcgca cgtttcaaaa
     4681 accacgccga catcatcgac ctgcaataca cctacaaatt caaataaacg ttacaccgtt
     4741 tgaatataaa aatgccgtct gaaacagatt tccgtttcag acggcatttt tatccggcag
     4801 ccgatacgcc tatctcggta ttcgggagaa tatatacaga acctccttca tatcaaaatg
     4861 gtagggacgc tcgggcaaca ggacggcggc aggcagcttg gtgatgccgt tcaaaccgcg
     4921 cgggaacagc gcaatccgct ctttgccctt ttccgcataa tctgctttgc ggttgtggat
     4981 ggaaactact tcgcgatggc ggtaaagcag cgtgttcatt tcgatatagg cttcgttgtg
     5041 gatggctgcc gactgtttct gcaacacgtc aatctgcctg ttgagcttgt cgttggcttt
     5101 catcagcctg ttgaagaacg acggttgctc ctgcagtttg gcggtacagg cttcgatact
     5161 ttccgatacc ggcccgaact gttcgaggta tttttccaga cagttttcca agtcggtcag
     5221 gcagccggcc atcttgcggg aaaattcttc ttcaggcttt ccctgccgaa tggacaatat
     5281 agcggattaa caaaaatcag gacaaggcga caaagccgca gacagtacaa atagtacggc
     5341 aaggcaaggc gaggcaacgc cgtaccggtt tttgttaatc cactatattt gggaggggcg
     5401 taaccccttc cgaatcagga tggcacatag ggcgacgctt tatgtgccgt cctgtgtgtt
     5461 gaaacatcag cataggaaac acaggaaaag ccggctgttt tgcgcctgcg taccgtattg
     5521 gtcaaaatgc cgtctgaaac cgacaacagg gtttcagacg gcatttccgt caattttccc
     5581 tttatctgcc gaacaggttg gcgatatttt ccatctgttc ttcggtaaaa ccgttttcgc
     5641 ccaatctggc ggcaactgcc cattcgcccg acaaaccgtg gtccgcaccg agctgccgcc
     5701 atttttcgac tttggcttcg gctgtggttt tttcgggcag cggtttgacg ggaatcctgc
     5761 ttttggggac ggtattcatc ggtttgccct aatcgatgga atagccgcga agatactgta
     5821 tccgttcgcg cgtcatccgc gtgtcgcatt ggagcttgag gtattcggcg tattcctgcc
     5881 ggtctgcctg cgcggcggct tgtcggcagt tgctgatttt ttcctgcgcc cacttgcgct
     5941 gttcgccgac caactctttt tgcacgtcgg tatcgagtcc tccccaaagt ttggtaattt
     6001 cggattccgc acgttggttt tgtacgcgcg cctcttccac ttcgccccgt gatacggtaa
     6061 cggtatcggc acgctcgacg tcgtcgggat gcaggatttc gggttcgggc gcgccttctg
     6121 cggcttgggg tacgcccgca tcgccgccgg cggcattgtg ttccaaaatg tcttcggggg
     6181 tgggtttgga cggttcttct tcacgggctt tgccgctcaa aaccctgacc gcgtcttctt
     6241 ttgtcaccgc cttgccgtct atcatcacga tgctcttcac gccgtaaggc agcaacgcgg
     6301 cagacagcgt ttgcgccgcc ataccgaccg tgttgtcgaa tgccgtccga gcgtctttgg
     6361 cgggcaggaa gcggacggct gccgtcaata cgccgtcttt aaactcgaca ttgccgcccg
     6421 tcttctgctg cacgatgtct gccaaagacg tttccccata cagcaggggg ctgtttgcct
     6481 cggcatcggc aagcgtttca gacggcacgg taatgttcaa atcggcgata cagaacgtgc
     6541 gcccgccttc ctgcgtttcc gaagcgtgtt ccaaagaaaa cgccaaaccg taggcggcgg
     6601 cgataatttt gtcggcatcg acaaactgcc tgccgtcttc gcgcgcgaaa gaacgcgctt
     6661 cctgcgtgag cgtttcctga atactgccgc gtatgtcctg caacacggcg gggttggcgc
     6721 attccaacgc cttgggcggt tcttccctgc cgcacgctgc aagcagcagg gcaaacggca
     6781 gcgcaatgag tttccgatac atgatgttcc ttccaaacgg tgcagaccga tggcggctat
     6841 catagcaaaa gcacctgcag gcggtaaacc ttgcaggcgc tttgtcggaa aggcggaaaa
     6901 tcagccttta aagaaattca caaaaccggt gagaatcgcg gcattaatta aatcgacaaa
     6961 gaacgcgccg accataggca caatcaaaaa cgccttatgc gacgcgccga aagtatgcgt
     7021 gacggactgc atatttgcca ccgccgtcgg cgttgcgccc aaaccgaaac cgcagtggcc
     7081 ggcagccaat actgcggcat catagtcgcg ccccataaag acataggtaa caaaagtcgc
     7141 gtacaaaacc atcactgcgg tttgtactgc aagaatcacg gttacagacc ccgccaaacc
     7201 ggtcagctcc cacagtttca aattcagcaa cgccattgcc aagaaaagcg aaagcgaagc
     7261 attgccgaac acatcgatgg cacggtcgaa catattgacc ttgaatgcgg cggtaaggat
     7321 gttgcggata accacgccgc caaacagaca ccacacgaat ttgggcaggt cgaacaggta
     7381 ttctttgtcg aagccgtcca taatctcggc aaacgccaga cacgcggcaa acatggcaag
     7441 cgtttcaacg gcagattccg ccgtaatcag gcgggtgcgt tttgcctgct cgaacacgtc
     7501 gtccgcgttg tcgtcctgat cctgttttgt gttttcaacc ggtttgcggc ccattttgtt
     7561 gatcaggcgg cgcgcaaccg gcccgccgat caggccgccg aacaccagcc cgaaagtagc
     7621 cgaagcaata cccaaaccgg ttgcgccgac caagccgtat tgcgtttcaa aattaggtcc
     7681 ccacgcacct gacgtgccgt gtccgcccgt cagcgacacc gaaccggtaa tcagaccgat
     7741 gagcgggtcc aaacccaaag ccgtagccag tccgaccccg acaaagtttt gcaccaagat
     7801 aaatccgccc acaatcgcgg taaaaaccac cagcggcaaa ccgcccgcct tcaaacggga
     7861 aaaatccgcg ctcaagccga tggacgtgaa gaaaatcagc ataaacgcat tttgcagcgg
     7921 tttctcaaat ttgaagctca cgccgtacgc ctcgtgcagc gcaaacagga taatcgcggc
     7981 aatcagcccg cccgccacgg gttcggggat gttaaaatca cgcaagattt tgattttctt
     8041 aaccaaaacc ttgccgacca acaaaaccaa agtggcggca atcaaggtgt aataactgtt
     8101 aaacgcccat tccatatcgt ttgacctcct aaaatgtttt tctttatatc cgcccgaagg
     8161 ggacgcaagc gcgaagatat taggggaata gttaaggctt gtcaaagaat cgccgttttt
     8221 tatagtggat taaatttaaa ccagtacatc gttgactcgc cttgccgtac tatctgtact
     8281 gtctgtggct tcgtcgcctt gtcctgattt ttgttaatcc actatacaac tattttttac
     8341 aaacaaaatg ccgtccgaaa ctccgttcag acggcatcat gggaatacac atatttgaaa
     8401 taacagaaaa agcgggggca aaacccgccg ctgcatctgt cgctgtacat ccctgctgtc
     8461 ggacttaccg cttcaacttc gcaaacgcat ccgccatcgc cgaattggtt gggacacgct
     8521 cgttgcgttg tggtttgcgg ccggctgtcc gttcgcggct gcggtttttt ccagctcgga
     8581 cagaatgtcc agaatggtcg gcagtccgaa gtgttcgtcg gtgaagtcgg acgctttgat
     8641 ttgcttcacg cgctcgcggt taactgccta aatatgaaat tcttggtctt cttgtagcat
     8701 aagttctcga tgatatgcca agtaagattt acgtttgtcg ttgagcagat tgttatctag
     8761 cttcacatca caccaacgtc gccaaacatc atctgataac cgcttagatt tcaccatatt
     8821 tccttcatca tcaaacgaaa tatatttcaa atcaaatagg ctatccaagg tacgacttaa
     8881 tagcaagccg ttatttgggt cgtatgcttc ggtatcatcc gacaggatga aaggcttaat
     8941 atgactggca atcaatacag gataggcaag tttttccagc atacatttca cattaccgta
     9001 atgttcttca ctttcttctt gaagttggtt tttgtatagg cgatgtaaat aagggtcgcg
     9061 tttccttaca gtaatttcat ccaaattacc aaaaatacgt tgggcatcct cagcaaaata
     9121 taagtcatca cctactcgtt ttaagtcgtc taatttgtct aataggttcc ataaataact
     9181 tatttgattg tattttcgtt caataaatcc tgattctatt ccctgttgaa aataatcatt
     9241 caattctgtt tcggttagat aatcttgttg aaaggttttt aagtctacta acatcatggc
     9301 ggcaatttct tttttattta gcttgccttg tggatgctcg accagcgttt taatcaggaa
     9361 atttatttgt cgaatattag agttttcagt tactgaacgc tgaaagccag agtgcgtata
     9421 tacgattttg gaaagtaggg tttgtcgttt tcggttagta cgagcttgta cataatctcg
     9481 tgataaaggt gtatagcttg ataaaaacgg ttctataaat cccattttta caaattgatt
     9541 aatagcttta cgaaccgagg ccaaattcat ggccttgctt gttctctgcg aagccataac
     9601 ggaatcttgg gcaatgtttt gctgcaatgc ttcatatagt tcgttgctgt atggctggtc
     9661 ttgattcgca tcaataaagt caagacaaat attgagtgca gtacaaaatt tttgatcgtt
     9721 ataatttgta aaggcattgg taaacgccca ataatcttca taaatttggc tgtttggctg
     9781 atta
//
LOCUS       DAXFZR010000082         9677 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000082 DAXFZR010000000
VERSION     DAXFZR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.083"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..211
                     /locus_tag="ACFJAS_001790"
     CDS             <1..211
                     /locus_tag="ACFJAS_001790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982964.1"
                     /translation="EETYPIEYIETRYAYQHYIKTQPIFFGAFYASNFVNKPFLQRPQ
                     ANKKQPSRFLQNIRKTKQMPSETTF"
     gene            complement(250..702)
                     /gene="rplI"
                     /locus_tag="ACFJAS_001791"
     CDS             complement(250..702)
                     /gene="rplI"
                     /locus_tag="ACFJAS_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC3982965.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEVNM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(719..949)
                     /gene="rpsR"
                     /locus_tag="ACFJAS_001792"
     CDS             complement(719..949)
                     /gene="rpsR"
                     /locus_tag="ACFJAS_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC3982966.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            complement(956..1258)
                     /gene="priB"
                     /locus_tag="ACFJAS_001793"
     CDS             complement(956..1258)
                     /gene="priB"
                     /locus_tag="ACFJAS_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC3982967.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            complement(1259..1627)
                     /gene="rpsF"
                     /locus_tag="ACFJAS_001794"
     CDS             complement(1259..1627)
                     /gene="rpsF"
                     /locus_tag="ACFJAS_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC3982968.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVGELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLVGAAEEAAAQ"
     gene            complement(1783..2733)
                     /gene="trxB"
                     /locus_tag="ACFJAS_001795"
     CDS             complement(1783..2733)
                     /gene="trxB"
                     /locus_tag="ACFJAS_001795"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC3982969.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            2872..5049
                     /locus_tag="ACFJAS_001796"
     CDS             2872..5049
                     /locus_tag="ACFJAS_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC3982970.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNYGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            5104..6990
                     /gene="uvrC"
                     /locus_tag="ACFJAS_001797"
     CDS             5104..6990
                     /gene="uvrC"
                     /locus_tag="ACFJAS_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC3982971.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSDQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            7107..8491
                     /locus_tag="ACFJAS_001798"
                     /pseudo
     CDS             7107..8491
                     /locus_tag="ACFJAS_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            8633..9349
                     /gene="trmB"
                     /locus_tag="ACFJAS_001799"
     CDS             8633..9349
                     /gene="trmB"
                     /locus_tag="ACFJAS_001799"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC3982972.1"
                     /translation="MTDTPENQTPNDLPAGHSRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
BASE COUNT         2527 a         2737 c         2358 g         2055 t
ORIGIN      
        1 ggaagagacc tatcctattg aatatattga aaccaggtac gcttatcaac actatattaa
       61 aacacagcca atattttttg gcgcatttta tgcatcaaat ttcgttaaca aaccatttct
      121 gcaaagacct caggcaaata aaaaacaacc gtcccgattt ttgcagaata tacggaaaac
      181 aaaacaaatg ccgtctgaaa ccacattctg ataatcggca gggtttcaga cggcatctga
      241 taatttcaat tactcggctg cggcaatgac ggcaacagta attttagcaa cggcatcagt
      301 gtgtaaagcc acttccactt cgtactctcc aacggctttc agaggaccgt tcggcagacg
      361 tacatttgct ttcacggctt cgatgccggc agcaacgatt gcggcagcaa tgtcggcatt
      421 ggtaacggaa ccgaacaggc ggccgtccac accggctttc tgtgcaacgg taacggtttg
      481 accgtccagt ttttcctgac gggctcgggc atctgccaaa atttcggcct gtttggcttc
      541 caattcggcg cggcgtgctt caaactcttt catgtttact tcagtagcac gttttgcctt
      601 accagcagga atcaagaagt tgcgggcata accgtttttt acggtaacga tgtcgcccaa
      661 attacccaaa ccgccgattt tttctaacag aataatttgc atgatttaaa ctccaaaatt
      721 atttgtgttg gtcggtataa ggcaggagtg ccaagaagcg cgcgcgtttt acggcaacag
      781 ccaattggcg ttggtagaat gccttcgttc ctgtgatgcg ggcaggaatg attttaccgt
      841 tttcagagat aaagtctttc agcaaatcaa cttgtttgta atcgacttct tggatttttt
      901 cagccgtgaa acggcagaat tttctacgtt tgaatgattg acgagccatt gtcgtttaac
      961 ctttatattc tttaatattt tgtatcctga gcatcggcat cagggaacgt ctgctttttt
     1021 gagctaaaaa accttcgacg tgaacatata caccttgccg atactgccac tcttccgcct
     1081 gcctacccaa aatccgcgcg gggatttcca attggacaag gcattgctgc ccattttcct
     1141 cctgccacga ttcgtgcttt aaaataatat ctaaaacagg gattccggca ggcgtatatc
     1201 gaatagggaa agccttttca atcaatgcgg caagcgaaac aagattagtg aatcccaatt
     1261 attgggcagc cgcttcttca gccgcgccga ccaacaggtt cttagccttt tcgccgccca
     1321 gcatagggga ggcttcggta acggcgtgtt tggttttgat ggtcagatga cgcaataccg
     1381 catcattgaa acggaacgcg gtttccaact ccccaacaac ttcgggagtg gtttcgatgt
     1441 tcatcaaaac ataatgtgct ttatggattt tgttaatcgg gtaagccagt tggcggcggc
     1501 cccaatcttc caaacggtga atcttaccgt ttgcctcggt aatcatggtt ttgtaacgtt
     1561 caaccatagc gggcacttgc tcgctttgat caggatgaac gataaacacg atctcgtaat
     1621 gacgcatgtt atctccttat ggatggtaaa aacagccttc tgccatgcga aagcagaagg
     1681 caaggttcaa atagcaggca ttatattggg gtttgccgac ggaatcaagg atttggtacg
     1741 aaaaacttgc attccgccga aaatttcggt ttcagacggc attcaaatgt tttggctgcc
     1801 cagccaacgt tccgcgtcca aagccgcctg acagccggag gccgcgctgg taattgcctg
     1861 acggtaggta tggtctttta cgtcgcccgc cgcccatacg ccttcgatat tggttgcacc
     1921 gacattgtcc gccgtgccgc cttttgtttt caggtagccg gcttcgtcca tttccaactg
     1981 ccctttgaaa atatcggtat tcggcttgtg cccgatggcg ataaagatgc cgctgacggc
     2041 aatttgttga tcagaaccgt cgttgttttt taataatgcg ccatttacgc cccgatcgtc
     2101 gcccagtact tcttgcaggt tgctttccag cttgaggatg attttgccct cttccacgcg
     2161 tttcatcagt ttgtcaatca tgattttttc agcacggaac tcgctgcggc ggtgaatcag
     2221 cgtaacggtt ttggcgatat tggcaaggta gagtgcctcc tcaactgccg tattgccgcc
     2281 gccgactacg gcaacatctt ggtttttgta gaagaaaccg tcgcaggtgg cgcaggcgga
     2341 aacgcctttc cctgcaaacg cttcctcact cggcaaacca aggtatttgg cggacgcgcc
     2401 ggtggcgaca atcagggcat cgcaagtgta ctcgcccata tcgcctttga gtgcgaacgg
     2461 gcgtttttgc agatcgacgg cgttgatttg gtcaaaaatg atttccgttc cgaaacgttc
     2521 ggcgtgggcg agaaaccgcg ccatcaattc cggcccttgc acgccgtcgg catcggcagg
     2581 ccagttgtcc acttcagtcg ttgtcatcag ttgcccgcct tgcgcgatac ctgtaataat
     2641 gacggggttt aaattggcac gtgcggcata gacggcggcg gtgtatccgg cggggccgga
     2701 acccaaaata atcagtttgc ggtgttggga cattgttttt cctttgctgt gtcaagtttt
     2761 cggattctac ccgaattatc ggcgcgtttg agaaattttg accataccgg cgttcagacg
     2821 gcatcccgca gccttgactg ccgtctgaac atcaaaacgg gaatcaaact tatgcaacaa
     2881 aaaatccgtt tccaaatcga ggggatgacc tgtcaggcat gtgcttcgcg cattgaaaaa
     2941 gtgttgaaca aaaaagattt tgtcgaatcg gcgggagtga actttgccag tgaggaagcg
     3001 caggttacgt ttgacggcag caaaacctcg gttgccgaca ttgccaaaat cattgagaaa
     3061 accggttacg gcgcgaagga aaaaacggaa gatacattgc cgcaacctga agcagaacac
     3121 catatcggct ggcggttgtg gcttttgctg accatcaata tcccgttcct tatcggtatg
     3181 gtagggatga tgctaaaagg gctgaattgg acacggcacg attggatgat tccgcctgta
     3241 tggcagtttg tactggcaag catagtgcaa ctttggctgg caatcccgtt ttacaaaagc
     3301 gcgtgggcaa gcattaaagg cgggctggcg aatatggacg tactcgttac catcggcacg
     3361 gtgtcgattt acctgtattc cgtttatatg ctgtttttca gttcgcatgc ggcgcacggt
     3421 atggcgcatg tgtattttga agcgggcgtg atggtgatcg gttttgtgtc gctgggtaaa
     3481 tttttggaac accgcaccaa aaaatccagc ctgaacagct tgggcttact gctaaaactc
     3541 acgccgaccc aagtcaacgt gcaacgcaac ggcgaatgga aacaactgcc catcgaccaa
     3601 gtgcaaatcg gcgaccttat ccgcaccaac tacggcgaac gcatcgctgc cgacggcatt
     3661 atcgaaagcg gcagcggttg ggcggacgaa agccacctta ccggcgaatc caatcccgaa
     3721 gagaaaaagg cgggcggcaa agtgttggcg ggcgcgctga tgaccgaagg cagcgtggtg
     3781 taccgcgccg cgcagctcgg cagccaaacc ctgctcggcg acatgatgaa cgcgctctct
     3841 gaagcacaag gcagtaaagc accgattgcg cgcgtggccg ataaagcggc ggcggtattt
     3901 gtgccaactg tcgtgggcat cgcgcttctg acttttatcg ttacttggct gattaagggc
     3961 gattggacgg tcgcactgat gcacgccgtt gccgttttgg tgattgcctg cccgtgcgcg
     4021 ctcggtctgg cgacccctgc cgcgattatg gtcggcatgg gcaaagcggt gaaacacggc
     4081 atttggttta aagacgcggc ggcaatggag gaagcagccc acgtcgatgc cgtcgtattg
     4141 gacaaaaccg gtacgctgac cgaaggcagg ccgcaggttg ccgccgttta ttacgttccc
     4201 gacagcggct ttgacgaaga cgctttgtac cgcatcgccg ccgccgtcga gcaaaacgcc
     4261 gcccacccgc tcgcccgcgc catcgtctcc gccgcacaag cgcgcggttt ggagattccc
     4321 gctgcacaaa atgcgcaaac cgttgtcgga gcaggcatta ccgccgaagt ggaaggcgtg
     4381 ggtttggtga aatcaggcaa agccgaattt gccgaactga ccttgccgaa gttttcagac
     4441 ggcgtttggg aaatcgccag tgcggttacc gtatctgtaa acggcaaacc gatcggcgca
     4501 ttcgcactct ccgacgcgtt gaaagccgat accgccgaag ccataggccg tctgaaaaaa
     4561 cacaatattg atgtctatat tatgagcggc gataaccaaa gtacggtcga atacgtcgcc
     4621 aaacaactgg gcatcgcaca cgccttcggt aatatgagtc cgtgcgacaa agccgccgaa
     4681 gtgcagaaac tcaaagccgc cggcaaaacc gtggcgatgg tcggcgacgg catcaacgac
     4741 gcgcccgcgc ttgccgccgc caacgtcagc ttcgccatga aaggcggtgc ggacgttgcc
     4801 gaacacaccg cctccgccac gctgatgcag cattcggtca atcagctcgc cgatgccctg
     4861 ctgatatcgc aggcaacgtt ggaaaacatc aagcaaaacc tatttttcgc cttcttctac
     4921 aatatattgg gcattccgct cgccgcgctc ggctttttaa atcccgtcat agcaggcgcg
     4981 gcaatggcgg caagctcggt ttcggtattg ggcaatgccc tgcgcctgaa atgggtaaaa
     5041 atcgattgac cgcatataac cgcccgacag cttttccgaa cggataaggc tgccgctgcc
     5101 gatatgccgt ctgaagccgt ttttcaaatg attgatatga atacagaaaa ccgttctccg
     5161 gaacaattcg acatcccgct cttcctcaaa aacctgccca agctgccggg cgtgtaccgt
     5221 ttttttgacg aaggcggcaa cgtcttatac gtcggcaaag cggtcaacct caagcggcgc
     5281 gtgtccggct atttccaaaa aaacgaccat tcgccgcgca tcgcgttgat ggtgaaacag
     5341 gttcgccaca tcgaaaccac catcacgcgt tccgaagccg aagccctgat tctcgaaaac
     5401 aacttcatca aagccttgtc gccgaaatac aatatccttt ttcgcgacga caaaagctat
     5461 ccttatttga tgctcagcgg ccatcaatat ccgcaaatgg cgtattaccg cggcacgctg
     5521 aaaaatccca accaatactt cggcccgtat ccgaacagca acgccgtgcg cgacagcatt
     5581 caagtgttgc aaaaagtctt catgctgcgt acctgcgaag acagcgtgtt cgaacaccgc
     5641 gaccgccctt gcctactgta ccaaatcaaa cgctgcaccg cgccctgcgt cggacacatc
     5701 agcgaagaag actactgcga cagcgtgcgt caagccgcca ctttcctcaa cggcaaaacc
     5761 gacgaactga cccgcaccct gcaacacaaa atgcagaccg ccgccgcgaa tctgcaattt
     5821 gaagaagccg cccgttatcg cgaccaaatc caagcactcg gcattataca gagcgaccag
     5881 ttcatcgaca gcaaaaaccc gaacaacccc aacgatatcg acctgctcgc gctcgccgtt
     5941 tcagacggcc ttgtctgcgt acactgggtc agcatccgcg gcggacggca cgtcggcgac
     6001 aaaagcttct ttcccgacac caaaaacgac cccgaaccaa acggtcaaga ttacgccgaa
     6061 gccttcgtcg cccaacacta tctgggcaaa agcaaacccg acatcatcat cagcaacttt
     6121 cccgttcccg atgcgttgaa agaggctttg gaaggagaac acggcaagca gatgcagttc
     6181 gttaccaaaa ccataggcga acgcaaagtt tggttgaaaa tggcggaaca aaacgcacaa
     6241 atggcgatta cccaacgcca cctgcaacaa agcaaccaac aacaccgcat cgacgaactt
     6301 gccaaaatcc tcggcatgaa ttcagacgga atcaaccgcc ttgaatgttt cgatatcagc
     6361 cacacgcaag gcgaagccac catcgcctcc tgcgtcgtgt acgacgaaca aaacatccaa
     6421 ccctcgcaat accgccgcta caacatcacc accgccaaac ccggcgacga ctacgccgcc
     6481 atgcgcgaag tgttgacgcg ccgttatggc aaaatacagg aagccgaagc caacggcgaa
     6541 agcgtcaaat ggccggatgt cgtgttgatt gacggcggta aagggcaaat cggcgtagcc
     6601 gtatccgtat gggaagaact cgggctgcac atccctttag tcggtattgc caaaggcccc
     6661 gaacgcaaag ccggcatgga agaactcata ctgcctttta ccggcgaact cttccgcctg
     6721 cctcctaata gcccggcctt gcatttattg caaaccgtac gcgatgaatc acaccgcttt
     6781 gccatcacag gccaccgtaa aaaacgcgac aaagcacgcg ttacctcgtc tctcggcgac
     6841 attcccggtg taggcagcaa acgccgccaa gccctgctta cccgcttcgg cggactgcgc
     6901 ggcgtgattg ccgccagccg cgaggacttg gaaaaagtgg aaggcatcag caaggcattg
     6961 gcggaaacca tttataatca tctgcattag tatgccgcca aagcaaaaac cgcctgtaaa
     7021 aatatgatac agcaggccgg cataccgata taaacctaac ttcatgacga ataacgatga
     7081 ttcgacaaaa cggaaaacga tctgatatga acaatcccga cttaccctat cggcaggcct
     7141 tggaacgcct gtctcaaaaa caatattata actttaccga agtccgccga ctgctgacag
     7201 aagcggcctc ggcagatcat cccgccgccg cattcaagtt ggcaaaacac ctgatgaacg
     7261 cggacagccc gcaccaagac cgcgaacaag gtatggaaat gctccgcatc gccgccgaac
     7321 aggggcatcc ctatgcgcgt tacaatttgg catatatcca agaattggaa ggcgcgcccc
     7381 cggaaaccct gataccgctt tacaggcctc tggcagaagc aggcctgccc gaagcgcaag
     7441 tccgcctgat gtaccttctg tacgcgtccc gacattttga agaagccctg gaatgggcaa
     7501 aacaagcgca aaaaacaaca atccctgcgg gcaatacctg ctcgcccaat actgccggca
     7561 cggcacgccg ccggattttg aaacggcgca cctgctctac cgcaaagcgg cggcacaagg
     7621 cttgccggaa gcacattggc aactcgggct gcaataccgt ttcggacaag ggacgaaagc
     7681 cgacacggca caggccgtca atcatttgcg cgccgccgcg caacaaggat acattcccgc
     7741 ctacaccccg cttgccgaac tcatcctacc tacggctccc gatgaagccg tttaccggtt
     7801 ccaacaggcg gcacaggaaa atgaccccga tgcccatgcc gcactggccg acatctacct
     7861 gcaaggcaag tatctggaaa gaaaccacaa acttgccctg catcatgccg aagcagccgc
     7921 cgccgaacgc catcccgaag gtttgcggat accgggcgac atctgccgct acggtttggg
     7981 catagccctc gatacggaaa aagcccggca ttattaccgg caggcagccg aagccggcag
     8041 ccttaccgcc tatcagaaac tcatatccga cagcgcgtta aaccatcccg agcaatatga
     8101 cggcatcaaa gattccgcca ttaggcggca aagggcagag cagctttatc aaaagcccaa
     8161 gccctgcatt acggattaca atgcgcaccc gaatacgcag ccgcgctcaa actgtacaca
     8221 gaagccgcag aactcggaca cagcaaagcc caaaccaatc tgggcagtat gtattacttc
     8281 ggacagggca cagccgccga ctacaacaaa gcccgcaaat ggtttgaaca agccacctcg
     8341 caaaaaaaca gtatggcgtt ctacaacctc gcctgcatcc attacagcgg acacggtgtc
     8401 aaaccggatt aaagaaaaag cctgtcactg cctgcaagaa gccataaaca acggatacgg
     8461 gcaaaaaaac gtcctgcaag aactgctgta acagtggcaa aatgccgtct gaacaccgtt
     8521 acacctaccc cgcaaaaacg aaacaggtat aatcgcccct ttccttcccg ccgtccgaac
     8581 agtcatttca cattcagacg gcatcctgat tgcacaagcg cacgaaagca ttatgacaga
     8641 cacccccgaa aaccaaacac caaacgacct gccggccgga cactcccgca gcatccgcag
     8701 tttcgtcctc cgccaaagcc acatgaccgc cgcacagcaa cgcgccatcg ataccttatg
     8761 ggacagcttc ggcatcgact accaagcaac accggccgat cttgatgccc gtttcggaag
     8821 cagccgacct aaaatcctcg aaataggctt cggtatggga atggcaagcg cagaaatcgc
     8881 ccgccgcctg cccgaaaccg actttctcgc catcgacgta cacggccccg gcgtaggcaa
     8941 cctgctcaaa ctcatcaacg aaaaccattt ggaaaacatc cgcgtgatgc ggcacgatgc
     9001 cgtagaagtt gtcgaaaata tgctgcaaga cggctcgctc gacggcatcc acatattctt
     9061 ccccgacccg tggcacaaaa aacgccacca caaacgccgt ctgatacaag cccccttcat
     9121 cgccaaacta ctgcccaaac tcaaaaccgg cggctatatc cacctggcga cagactggga
     9181 agaatatgca cagcagatgc ttgaagtcct cagcagcttc gacagcctgc aaaatacggc
     9241 ggcagactac gcccccactc cggactaccg ccccgaaacc aaattcgaag cgcgcggcaa
     9301 acgcctcgga cacggcgttt gggacttggt attcaaacgg atcggataac agaccgccgg
     9361 ataaaaaatg ccgtctgaag catgtttgct tacagacggc attctttcaa gataaagcag
     9421 cgggcgatgt ttcaatacaa gttttgaaac aatggtttga acggcaaaaa cgcgtgtata
     9481 ccgcacgcat ccttgtaggt tttaacctgc acatcggttt taaagtttat tccgcccgca
     9541 gatagtggca cacggttggc ggagatgcag gctacggctt gctaattatt catcgttacc
     9601 aaaagaagac ttaggtgtac attcaggcat agttaacaaa acaactaaac ttctatttac
     9661 tggaaaataa aatgaca
//
LOCUS       DAXFZR010000083         9273 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000083 DAXFZR010000000
VERSION     DAXFZR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.084"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..117
                     /locus_tag="ACFJAS_001800"
     CDS             <1..117
                     /locus_tag="ACFJAS_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC3982973.1"
                     /translation="PEPNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
     gene            complement(225..3077)
                     /gene="gcvP"
                     /locus_tag="ACFJAS_001801"
     CDS             complement(225..3077)
                     /gene="gcvP"
                     /locus_tag="ACFJAS_001801"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC3982974.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRSVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3108..3467)
                     /locus_tag="ACFJAS_001802"
     CDS             complement(3108..3467)
                     /locus_tag="ACFJAS_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982975.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3466..3645
                     /locus_tag="ACFJAS_001803"
     CDS             3466..3645
                     /locus_tag="ACFJAS_001803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC3982976.1"
                     /translation="MPSEPAGRLSQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3638..4279)
                     /locus_tag="ACFJAS_001804"
     CDS             complement(3638..4279)
                     /locus_tag="ACFJAS_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC3982977.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4269..4820)
                     /locus_tag="ACFJAS_001805"
     CDS             complement(4269..4820)
                     /locus_tag="ACFJAS_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC3982978.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4824..5342)
                     /locus_tag="ACFJAS_001806"
     CDS             complement(4824..5342)
                     /locus_tag="ACFJAS_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC3982979.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5342..7003)
                     /gene="pqiB"
                     /locus_tag="ACFJAS_001807"
     CDS             complement(5342..7003)
                     /gene="pqiB"
                     /locus_tag="ACFJAS_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC3982980.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQVKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6996..8456)
                     /locus_tag="ACFJAS_001808"
     CDS             complement(6996..8456)
                     /locus_tag="ACFJAS_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFC3982981.1"
                     /translation="MGLSAGGAAAQKIFLPDTKGLFILPNLPVLHPDWVYRFFSFIMP
                     PFIFVTFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRTDVPQLDKGEAAFCPR
                     CGHKLFRVGSHPFSGPPAYAAASLILMAFAYSMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAKVRFGPAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGNN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVVLYFPA
                     NILPIMISSNPAATEANTIFSGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     AARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8641..9270
                     /locus_tag="ACFJAS_001809"
     CDS             8641..9270
                     /locus_tag="ACFJAS_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC3982982.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
BASE COUNT         2072 a         2392 c         2637 g         2172 t
ORIGIN      
        1 cccgagccca acccgattga gaaagtgtgg gcgaatatta agcggtatct gcgaaccgtt
       61 ttgtctgatt acgcccgatt tgacgatgca ctactgtcct attttgattt taattgacta
      121 taaagttata gtcgagatac gtgtatcggt gcaaccggtc tgccgttgac gaaaatgccg
      181 tctgaaagtg tttcagacgg catttgtctt cggggtttca acagtcagtc ttcataattt
      241 tccatcggcg ggcagctgca cacgaggttg cggtcgccgt acacgtcgtc cacgcggttg
      301 acgaagggcc agaacttgtg ttcgcgcacg aagggcagcg ggaagacggc ttcttcgcga
      361 gagtacggat gcgcccattc gccggttata tctgcggcgg tgtgcggcgc gttgaccagt
      421 gggttgtcgt ctttcggcca ttcgccgtcg atgactttct gcacttcgcg gcgaatggat
      481 ttcagggcgg cgatgaagcg gtcgagttcg gctttgcttt cgctctcggt cggttcgatc
      541 atcagcgtgc cggcaacagg gaaggaaacc gtcggcgcgt ggaagccgta gtccatcagg
      601 cgtttggcga tgtcggtttc ggtgatgccg ctttcggctt tgagcggacg caaatcgacg
      661 atgcattcgt gcgcgatgcg gccgtttttg cctgtataca gaatcggata gtcttcgctc
      721 aagcgtttag cgacatagtt ggcgttgagc aatgcccagc gtgttgcctg ttccatgcct
      781 tgtttgccca tcatggtcag gtacatccaa gtaatcggca ggatggatgc agaaccgaag
      841 gctgcggcgg caacggaggt ttgcccggca cttgcgctgt gggtgtcggt cagggtatgc
      901 cccggtgcga acggggcgag gtgggctttc aagccaatcg gacccatgcc cgggccgccg
      961 ccgccgtgag ggatacagaa ggttttgtgc aggttcatgt gcaacacgtc cgcgccgact
     1021 tcggcgggct gcatgatgcc gatttgggca ttgaggtttg cgccgtccat gtaaacctgt
     1081 ccgccgtttt cgtgaatgat ccggcagatg tcgcggatgc cttcttcgta cacgccgtgt
     1141 gtggacggat aggtaatcat gatggcggac aaggcatcgc ggtgttgttc ggctttggct
     1201 ttcaaatcgt caatattgac gttgccgtgt tcgtcggtat cgacgacgac gactttcaaa
     1261 ccgagcatgg cggcggtggc ggggttggtg ccgtgggcgg atttgggaat caggcagatg
     1321 ttgcggtgtg cttcgccttg ggcttcctga taacggcgga tggcgagcat accgctgtat
     1381 tcgccttgtg cgccggagtt gggctggaag gaaatcgcgt caaagccggt gatggctttc
     1441 aggctgtttt ccatatcagc aagcaattcg cggtagccgg cggtttgcgc ttcgggagcg
     1501 taagggtgga tgtcggagaa ctcagtccaa gtaatcggca acatttccgc agtcgcattg
     1561 agcttcatgg tgcagctccc gagcgaaatc atgctgcggt tcatagccaa gtcgcggtct
     1621 tcgagttttt tcaggtagcg caacatttcg tgttcggtgt ggtaactgtt gtacacagga
     1681 tgttgcagaa tgtcgtcttg acgcagtaat tcggcattca gacggccttt gacatcatcg
     1741 gcaaatgtga acgtatcctt gccggtaaac gcgcggtaca aatcggccaa atcttcgtat
     1801 accgacgttt catggaatgc agccgcaact tgagtattgt tgacgctgcg caggttgtaa
     1861 cccgactcca aagcagcggc aaacacttgg tctgctttct ctttgctgcc gaaatcgacg
     1921 gtaacggtat cgaagaagac ttcgtgaacc actttgaggc cgtctgaaac cagcgcgtcg
     1981 gcaaagacgg aagccagtgt gtgaatgcgg ttggcgatgc gtttcacgcc tttggggccg
     2041 tgataaacgg cgtacatgcc cgccaagttc gccagcaatg cctgtgcggt acaaatattg
     2101 gatgtcgctt tttcgcggcg gatgtgttgt tcgcgggtgg acaaagccat gcgcaaggca
     2161 ggtttgcccg atgcgtcttt ggatacgccg atgatgcggc ccggggcgga gcgtttgaac
     2221 tcgtctttaa acgcgaaata agcggcgtgc ggaccaccaa agcccatcgg tacgccgaaa
     2281 cgttgggtgt tgcccaaagc aatatctgca cccaattcag ccggagactt cagcaaaacc
     2341 aagctcatga tgtcggcggc aacagcaacg attgtgcctt tggctttcag acggccgata
     2401 acgtcctgca agtcttgcac gtcgccatct ttaccgacgt attggaacag cgcgccgaag
     2461 tattcgcctt catccgcttg ggcaaaatcg ctgaccacca gctcgaagcc gaaatatttg
     2521 gcgcgggttt tcatcacgtc caaagtttgc ggatacacgc gcgcatccac aaagaaacgc
     2581 tcggatttca ctttgcccac gcggtgcgcc atcgccatcg cttcggcggc ggcggtcgct
     2641 tcgtccaaca aagacgcgcc cgccacaggg aaaccggtca aatcgatgca cacctgctgg
     2701 aagttcaaca acgcttccaa acgaccttgg gcaatctccg cctgatacgg cgtgtaggcg
     2761 gtgtaccagc ccgggttttc caatacgttg cgcaaaatca cgttcggcac gcgggtcggg
     2821 taataaccca agccgatata ggacttgttg atcacgtttt tcgacgcgat gcctttcaat
     2881 ttcgccaatg cgtccgcctc ggtcagggct tcgggcaaat cgagttcgga cggcatacgg
     2941 atgctttgcg gcaaggtgtt gccgacaaag tcgtccatgc tcttctcgcc gacagcggca
     3001 agcaacgccg cttcgtcgcc gaaactcaaa tggcgcgcgg caaattcgtt ggggttgaac
     3061 agttcggaca gtttcatttc tactccttcg ggtttgtagg gctgccatca ggcagaattt
     3121 ttagacatcg gatgattcgg gcaagggtgg cacaaaaggc gggataatgg cggacaacgc
     3181 gccagaatgc agcaggtcgg gacaagcagc gccacaagcg gcagcgataa gggaaagccg
     3241 ccgcccaaaa ccatgaaacc tgcaggcacc agccaagcca agggatgagc cggaagcgga
     3301 aaaacaacat tgagcagaac caaaatcacg acaaacagcg gcagccccgc catcaggttg
     3361 gcacgcctgt atgttgcgta cgggtcggat gcttcttcag gttcgcaata ttccatgatt
     3421 tcagcctgtc cgggctttat ccgcagcagg ttcggacggt cggtcatgcc gtctgaaccg
     3481 gcaggccgtc tttctcaaaa aacgccatac cgctgcaact tcatgcggct gtatggcgta
     3541 ggttttgcac ttcagacggc atatgcgtcc cgaagcagtt tggtgtccca gtcggttcat
     3601 gattatataa acaacttggg tgcggataaa cctttgttta tctaaaccca tgttttctca
     3661 aaaattgatt caaattttcg gcgaatttcc gatagccttt gccgttggcg tggatttggt
     3721 cggatttcag attattattg cccaaaattt ccgcccacgc gccgccgaac aacggaatgc
     3781 cgtattcctc ggacaaatcc tcatacagcg gatggtcgct caaatgcccg aacaacgcgc
     3841 ccagtgtgat gtgcggcacg ccgacgagga cggcgggaat gttttccttt tgcacggttt
     3901 cgatgatttt cgcgatattg gcgcgggtct gctcctcggg aactttgcgc agaaagtcgt
     3961 tgccgcctat gccgacaatc acaagcttgg gtttgcgtgc caacagcgcg ggcaggcgcg
     4021 atagggcttg cgcggacgta tcgcccgata cgccgccgtt gacaatattc caacccgtca
     4081 gtttttgcag ttgcgcgggg taggattcgc cggggtttgc tccgtagccg aaggtgagcg
     4141 aatcgcccaa ggcaagcacg gtgcttcctt cgggaatttt ggcgtgggtt cgggcggatt
     4201 ttctgccgca ggcggtaaga agcaacgcgc ctgcgccgag gaggaaggtt cttctgttca
     4261 ttggtttttc agacggcatc gagccatttc gggtggtatt tcagggtttt acggtaaacc
     4321 ggacagcttt cggcgtgcgc gcctttgagg tagccggtac tcatcagaaa ttcgccgacg
     4381 atttcgccgc cgacgaattt gaaatgtttt ttaaagagtt tgacccattc gtctttgctt
     4441 cgcggatggt gcgcgtcgag ccagttcttg aacgaaccgt gttcttgttg caacgcttgg
     4501 atttgccgtg cattgaaaat ggcggcatcg attttcagac ggttgcggac aatacccgca
     4561 tcggtaagca gccgttcaat atcggcttcg ccaaatgcgg cgaccgtatc gatgtcgaaa
     4621 ccttcaaatg ccgtctgaaa cgcctgccgc ttcttcagca tcagcgtcca acttaatcct
     4681 gcctgattga tttccaacac cagccgttca aacagtccgt tgtcgtcctt aatcggaaaa
     4741 ccgtattgcg tatcgtggta atgtttgttc gggttgtcgg tgttttcggg aagtgaggcg
     4801 gcaaattcgc aatagttcac ggtttactcg accatctgtt gcgccgcctg tttcagtccc
     4861 tgttcgagtg cggcggtcat ggcggcgtag ccgtcaccct gctgttcggt ttcgatatgg
     4921 aagggtctgt tcgtaccgtc gggtaggacg gcgtagccgc tgatgagggt tttgcccgtg
     4981 tagctgcctt ggaatgcgtc gatatagacc gtccatttgt cggtactgcc gctgcgtgag
     5041 gcaggaacaa aggtgcgtgt gctgtccaaa cggttgaatg cattgctcaa cgccgcttcg
     5101 agcatatcgt ccaaggtgtc tgcccaaaca tggttttgtg cggtgttgat gcggtagggg
     5161 tcggtttgat agaccagtcc gccgcgtttg agcggttcgg caagacggac ttcgacggcg
     5221 gtttcgccgc cttgcgttgc aggacggatg tagcggctgt cgggcaacac gaaatattgt
     5281 gtgctttgca cagtaccgca ggcggcaagc gtcagggcgg cggcgatggg gaaaaggcgc
     5341 attatcggct tcctttcggg atagggtctt tgctgctgtt gttgaaaatc agtgcgttgg
     5401 gtttttcttt caaagtgtta atgacgggtt gaacgtcttt taaggttttg tccaaacttt
     5461 gcagcgtatt ttgtacgtct ccgtagatag gcgattgagg cgatacgcct tgcagggtta
     5521 tgcgcaactc tttcagagtt tggttcagtt cgttcgggat gttttgcgtc tgcggattgc
     5581 cgaccagttt gtcaatggag cttagggcgg cattggcgga tttgagtgcg gacttgagtt
     5641 cggcgagcga gccgttcaat tcggcaacgg ttttatccaa tggcagattg ttgaatttgt
     5701 ccagcaaatc cgccaatttg acctgcaagt cgtccaaacc gccgccccgt gtggcgatga
     5761 cggtatcgcc tgcataaacg gtatgcggtc gcagcttggg cgaggcggaa ggctgatcgt
     5821 tcaactcaat cattttgccg ccggtcagca ggttgttgct ggagatggtg gcggtcaggc
     5881 ctttgtttaa ggccgtctgg aattgttgtt tccaatgctc tttgctttgc tcgtcggcat
     5941 tgatttccaa acgggaaggc tcgatgcgga tgcgtacggg aatccagccg ttttcaaaca
     6001 ggtgcaggct gtcattgcgg tcaaaataag ggacatcgga aaccatgccg acattcagcc
     6061 ctttgtattc gacaggcgaa ccgacggtca gtccgcgcac ggattgtttg aaaaacgcgg
     6121 tgtagtacag cgagcggtcg tcaggcaggt tggcgatttc gctgcggctg tcgtaaagcg
     6181 tgaagctgtc ctcgcttttg acgtttttac tgtttttggt tttcggcgaa tcaaatgaaa
     6241 tcgcgcctga cagcagggca ggcagagggg cggaattgag tttgatgccg ctgcctgtgg
     6301 tttcgatatt gatgccgctt tccagccaaa aacggctggc ggaatgaatc agtttgtcgt
     6361 tggggctttg gatgaagatg gtgtaatgca cgctttggtc ggacgggtcg aaatgcgcgc
     6421 tttcgatttg cccgaccata aagttttcat acaaaacagg gctgttgacg ttgaggatgc
     6481 ggtcgttttt accaatcaaa ttcaagcgca gcccgctttg cccgatggcg gtaacgggcg
     6541 gaatgtcctg cacttggaac acgtcttttg cctcgccgct tttgccgggt gtaaaagcga
     6601 tgtacgaacc cgaaagcagc gtacccaaac cggttacgcc gctttggtcg atacgcggct
     6661 tgaccaccca gaactgggta tcgctgcgga tgaggccgga tacgtccgca ttgagttggg
     6721 cagtaacttc cacgcctttt tggtcgtcgc gcagtttgat tcgggtaacg cgtccgacat
     6781 cgatgctcaa taccttaatg accgtattgt tgacttcgat gccttccgcg ctgtccatca
     6841 agagcgtaac cacaggcccc ctgttgcgga tttccttaac ccaaagccag ccgccggcaa
     6901 tcagcgcgat cagcgggacc agccagacgg cggagaggaa ggtgttgttt ttgcggacgc
     6961 gtgcttgagc gtgtccgttt ggaggagggc tgttgtcagt catatttttc cgtttcgttg
     7021 aaagcaatgc cgtctgaagc gcgtttgtcc caaagcaggc gcgggtcgaa ataataggcg
     7081 gacagcatcg tcaaaatcac gaccaggcag aaatagactg ccgcactgcc cggaatgacg
     7141 cgcgcggcat aagtgtggaa cgaacacatc aaaataataa tcacaaaaat atcaatcatc
     7201 gaccagcggc cgaccgcttc ggtgatgcgg tagaggtgcg acaatttctt tgcgcccgcc
     7261 ggcaaagcga accgtgccgc cgcaatcaaa accgacattg ccgcaatctt cagcaccggc
     7321 accaaaatac tcgcgctgaa aataaccgcc gcaatcagcc tgtcgccctc gtcccacata
     7381 taagcgatgc cgctaaagat ggtgttggcc tccgtggcgg caggattgga cgaaatcata
     7441 atcggcagga tattggcagg gaaatacaaa acaaccgccg ccgtcagaaa cgccgacgaa
     7501 atactcagac ttttcggccg tcggcggtac agttccgcgc cgcacacccc gcagggggat
     7561 tcggcactgt cgcggaaata caggcagcgg ctgcaacagg ttttgccttc cgatgccgtc
     7621 tgaaccgcat tattccccgt cagccgcccg atttggaaat acacccaatg ctggggaacc
     7681 gataccgaag tccgaatcag cataaccgac agcgcgaaca tcagataaaa cgccggcccg
     7741 aagcgaacct ttgccacaga cgagagcttg atatacgcca ccagagtgga aacaaaaaac
     7801 acatccacca tcatcgcctg cctcaagcgc accatcacac gcgttgccaa acgcagcgca
     7861 ggatacgcct gtttccgtat cagcgcggca tagacataca ggcacagcag cagaaacaga
     7921 accggcgcgc cgaaagtcag cacaaacatc acttcggcca aaaaaccata atcctgaaac
     7981 accatcaggc gcatcatctc gggcagcgaa aggacggatg ccgcacccgg tatcccgacc
     8041 tcgatatacg tcatactgta agcaaacgcc atcaaaatca gcgaagccgc cgcataggcg
     8101 ggcggaccgg aaaaagggtg gctgcccacc ctgaagagtt tgtgtccgca acggggacag
     8161 aacgccgcct ctcccttgtc caactggggc acgtctgtac ggcagccgca ttccgggcaa
     8221 tctaccgtat gcggcggcag ggcttcgtcg cggcagttgc cgcgaaggct gtgggggata
     8281 ttttcggcaa acggcttcat cgggaaagtg acaaatataa atggcggcat tataaaggaa
     8341 aaaaagcgat acacccaatc aggatgcaaa accggcaggt tcggcagtat gaacaaaccc
     8401 ttggtgtccg gcaaaaatat tttttgagcg gctgcccctc cggccgacaa gcctatttga
     8461 taaaaccccg attttgtaaa taatcataaa atacccgctt aaaaatataa cgcaaatgat
     8521 attttttcgt aaaatatgtg ataataaatt tcgtttatga ttctttatta tttttgtatg
     8581 cgggcaagtc cggattttgc ccgcaccgca tccttatccc ttaccgacaa aggagaactt
     8641 atgagcgaaa ccgaaaacca agccctgacg tttgccaaac gcctgaaggc ggataccacg
     8701 gcggttcacg acagcgtgga taacctcgtt atgtctgtcc aaccgtttgt cagcaaagaa
     8761 aactacatca aatttttgaa actgcaatcc gtgttccaca aagcggttga tcatatctat
     8821 aaagaccccg agttgaacaa agccatttcc gagctggaat acatggcgcg atacgatgcc
     8881 gtaacgcaag acttgaaaga cttgggcgaa gagccttaca aattcgacaa agaattgcca
     8941 cacgaaaccg gcaacaaagc cgtcggctgg ctttattgcg ccgaaggatc caatttgggc
     9001 gcggcatttt tgttcaaaca cgcgcaaaag ctggattaca ccggcgaaca cggcgcgcgc
     9061 cacctcgctc cgcatccgga cgggcgcggc aaacactggc gcgccttcgt cgagcacctg
     9121 aatgccctga acttaactcc ggaagcagaa gcagaagcca ttcaaggcgc gcaggaagcg
     9181 tttgctttct ataaagtgat tctgcgtgaa accttcggtt tgccggaagg aacagaagcc
     9241 cccgaaggca tgatgccgca cagacattaa aaa
//
LOCUS       DAXFZR010000084         8416 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000084 DAXFZR010000000
VERSION     DAXFZR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.085"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            109..3324
                     /gene="carB"
                     /locus_tag="ACFJAS_001810"
     CDS             109..3324
                     /gene="carB"
                     /locus_tag="ACFJAS_001810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC3982983.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNRS"
     gene            complement(3525..4565)
                     /gene="queA"
                     /locus_tag="ACFJAS_001811"
     CDS             complement(3525..4565)
                     /gene="queA"
                     /locus_tag="ACFJAS_001811"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC3982984.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            4682..4939
                     /locus_tag="ACFJAS_001812"
     CDS             4682..4939
                     /locus_tag="ACFJAS_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC3982985.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            4921..5340
                     /gene="accB"
                     /locus_tag="ACFJAS_001813"
     CDS             4921..5340
                     /gene="accB"
                     /locus_tag="ACFJAS_001813"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC3982986.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEVGQQVKAGDTLCIIE
                     AMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            5454..6815
                     /gene="accC"
                     /locus_tag="ACFJAS_001814"
     CDS             5454..6815
                     /gene="accC"
                     /locus_tag="ACFJAS_001814"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC3982987.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            6919..7806
                     /gene="prmA"
                     /locus_tag="ACFJAS_001815"
     CDS             6919..7806
                     /gene="prmA"
                     /locus_tag="ACFJAS_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC3982988.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRSGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLNGVKR"
     gene            7824..8387
                     /gene="orn"
                     /locus_tag="ACFJAS_001816"
     CDS             7824..8387
                     /gene="orn"
                     /locus_tag="ACFJAS_001816"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC3982989.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         2044 a         2545 c         2211 g         1616 t
ORIGIN      
        1 aattcttgga aacttatcaa tcatgggaaa agttacagca aaagtatcct aatattacat
       61 tttcgtatga taaaaatgat ttaattttaa ctaaatagag acccacccat gcccaaacgt
      121 accgacctaa aatccatcct tatcatcggc gccggcccta tcgttatcgg tcaggcctgc
      181 gaatttgact attcgggcgc acaggcctgc aaggctttgc gtgaagaagg ctataaagtc
      241 attctggtga attccaaccc cgccacgatc atgaccgacc ccgaaatggc ggatgttacc
      301 tacatcgagc cgattatgtg gcagacggtg gagaagatta tcgccaagga gcggcccgat
      361 gcgattctgc ccacgatggg cggtcagacc gcgctgaact gtgcgctgga tttggcgcgt
      421 aacggcgtgc tggcgaaata taatgtcgag ttaatcggcg caacggaaga cgcgatcgac
      481 aaggcggaag accgcggccg ctttaaagaa gcgatggaaa aaatcggcct ctcttgcccg
      541 aaatcttttg tctgccacac catgaacgaa gccttggcgg cgcaagaaca ggtcggcttt
      601 ccgacgctga ttcgtccgtc tttcacgatg ggcggttcgg gcggcggcat tgcctacaat
      661 aaggatgagt ttttggcgat ttgcgaacgc ggtttcgatg cgtcgcctac gcatgagctg
      721 ctgattgagc agtctgtgct cggctggaaa gagtacgaga tggaagtggt gcgcgataag
      781 gcggacaact gcatcatcat ctgttcgatt gaaaacttcg acccgatggg cgttcatacg
      841 ggcgactcga ttacggttgc gccggcgcaa acgctgacgg acaaggaata ccaaatcatg
      901 cgcaacgctt cgttggcggt attgcgcgaa atcggcgtgg acacgggcgg ctcgaacgtg
      961 cagtttgcgg tgaaccctga aaacggcgag atgattgtga tcgagatgaa cccgcgcgtg
     1021 agccgttcgt ccgcgctggc ttccaaagca acgggcttcc cgattgcgaa ggtggcggcg
     1081 aagctggcgg tcggctttac gctggacgag ttgcgcaacg acatcaccgg cggccgcacg
     1141 cccgcgtcgt tcgagccttc catcgactat gtggtaacca aaatcccgcg tttcgcgttt
     1201 gaaaaattcc ccgccgcaga cgaccgcctg accacgcaga tgaaatctgt gggcgaagta
     1261 atggcgatgg gccgtaccat tcaggaaagc ttccaaaaag ccttgcgcgg tttggaaacc
     1321 ggcttgtgcg gtttcaatcc gcgcagcgaa gacaaagcgg aaatccgccg cgaactggcc
     1381 aaccccggcc ccgaacgtat gctgtttgtg gcagacgcgt tccgcgcggg cttcacgccg
     1441 gaagaaatcc acgagatctg cgccatcgac ccttggttct tggcgcaaat cgaagacttg
     1501 atgaaggaag aaaaatcggt ttcagacggc cagttgcaag atttggatta cgccgcccta
     1561 cgccgtctga aacgcaaagg cttctccgac aaacgtttgg cacaattgtt gaacgtaagc
     1621 gaaaaagaag ttcgcgaaca ccgctacgcg ctgaagctgc atccggttta caaacgcgtc
     1681 gatacctgcg ccgccgagtt cgccaccgaa accgcctatc tttactccac ttacgaagaa
     1741 gaatgcgaat cccgtccttc cgaccgtaaa aaagtgatga ttctcggcgg cggcccgaac
     1801 cgcatcggtc agggcatcga gtttgactac tgctgcgttc acgccgcact cgccctgcgc
     1861 gaatcgggct ttgaaacgat tatggtcaac tgcaaccccg aaaccgtgtc caccgacttc
     1921 gacaccagcg accgcctcta tttcgagccg ctgacattgg aagacgtgtt ggaaatcgtc
     1981 cgcaccgaaa acccgtgggg cgtgattgtt cattacggcg gtcaaacccc gctgaaactc
     2041 gccaacgcat tggttgaaaa cggcgtgaac atcatcggca catccgccga cagcatcgat
     2101 gccgccgaag accgcgaacg cttccaaaaa gtgttgaacg acttaggcct gcgccaaccg
     2161 cccaaccgca tcgcccacaa cgaagaagaa gcactcgtca aagccgaaga aatcggttat
     2221 ccgctggtcg tgcgcccgtc ttatgttttg ggcggacgcg cgatgcagat tgtccactcc
     2281 gccgaagcct tgcaaaaata catgcgcgaa gccgtgcagg tttccgaaga cagccccgtg
     2341 ctgctcgatt tcttcctcaa caacgcgatt gaagtcgatg tggactgcgt ttcagacggc
     2401 aaagatgtgg ttatcggcgg catcatgcag cacgtcgaac aggcgggcat ccactccggc
     2461 gactccggct gctcgctgcc gccctactcc ttaagcgaag aaatccaaga cgaaatccgc
     2521 cgccaaacca aagcgatggc gtacgcgctg ggcgtggtcg gactgatgaa cgtgcagttt
     2581 gccgtgcagg acggcgtggt gttcgtgttg gaagtgaacc cgcgcgccag ccgtaccgtc
     2641 ccctttgtct ccaaagccac cggcgtgccg ctcgccaaag tcggcgcgcg ctgcatggca
     2701 ggcatttccc tgaaagaaca aggcgtggaa aaagaagtcg tccccgattt ctatgccgtt
     2761 aaagaagccg tgttcccatt catcaaattc ccgggcgtgg atacgatttt gagtccggaa
     2821 atgcgctcca ccggcgaagt gatgggcgtg ggcgcaagtt tcggcgaagc ctactacaaa
     2881 gcccaactcg gcgcgggcga acgcctcaac ccgaccggca aaatcttcct ctccgtgcgc
     2941 gaagaagaca aagaacgcgt cattaaaacc gccaaaaact tccaagcctt aggctacggc
     3001 atctgcgcca cgcgcggcac ggcgcaatac ctgaccgaac acgggctgat tgtgcaggcc
     3061 atcaacaaag tccccgaagg ccgcccgcac atcggcgacg cgctgaaaaa cggcgaaatc
     3121 gcactggtcg taaataccgt ttccagcgat ccgcaatccg tgtccgacag ccacatcatc
     3181 cgccaaagcg cattgcagca acgcgtgccg caatacacca ccaccgccgg cggcgaagcg
     3241 atgagcgaag gcgcgaaaag ccgggattat ctgggtgtgt acagcgttca agagctgcac
     3301 ggtcgtttga aaaaccgcag ctgatgcctg aatcaggttg aacaatgccg tctgaagccg
     3361 ttttgcggtt tcagacggca ttttgttgca catagccttc actttctttt gacctttaag
     3421 caccctaatt tggggcggtg gattatccaa ctgccggaca tccataaaaa taccgcccga
     3481 accacggttc agacggcatc tgccttgcaa cggacagcag cagtttaaag cccgccccct
     3541 tcgttccgcc ccaaaaccat cgcatctccg tagctgaaga aacggtattc acgttcaatc
     3601 gcatgacggt acacggcgcg gatatgaccc atacccgaaa acgcgccgac caacatcagc
     3661 agcgtcgatt tcggcagatg aaaattggta accagcctgt cgacaacatt aaaacggtag
     3721 cccggcgtga tgaaaatatc ggtgtcgccc tgtccgtctt tcaaatatcc cgttgcgcgc
     3781 gcggcagact cgagggcgcg catggaagtc gtgccgaccg cccaggcttt gttcccccgg
     3841 gcttttgccg cctcaacggc ggcgacggtt tcagacggca cttcaaacca ttcgctgtgc
     3901 attttgtgtt cttcgatttt atcgacgcgc acgggttgga atgtccccgc accgacgtgc
     3961 agggttactt ctgcggttac cgcgcctttg tctttcagac ggcgcaaaag ttcttccgta
     4021 aaatgcaggc ccgccgtcgg cgcggcgacc gcgccctgat atttggcata aacagtttga
     4081 taacggctgt cgtcgtccgc atcggcggca cgttcgatat aaggcggcag gggcaggtgt
     4141 ccgttctgtt ccaaaagttc gtaaacggtt tgaccgcctt caaaacgcag gcagaacagt
     4201 tcgccctcac gcccgaccat cacggcacgg ataccgcctt caaacaccag ccccataccg
     4261 ggcttggggg acttggacga acggatgtgc gccaatgcgg tatggttgtc caacacacgc
     4321 tcaatcaggg cttcgatcct gccgccgctg tctttctgcc caaacagccg cgccttcatg
     4381 actttggtgt tgttgaatac caaaacgtcg cctgcctcga cataatcggg caaatcgcca
     4441 aacacccggt cttgcagcgg catatcgggc agcgcgacca aaagccggct gctgccgcgc
     4501 acctcgggcg gatgctgggc aatcaggtgt tcgggcaggg taaagtcaaa atctgaaata
     4561 tccattttta cactctcgtt cgggcaagcc gccattatac gcactttagc cctttttcag
     4621 acggcatctt tgcccgaaaa accaacagat tagaataaac actcttgact cggggcatct
     4681 tgtgcgcaaa atcaaacttc ctgcacattt cccccaaaaa ccgccgtttt ttgatatttt
     4741 actggacatt taccgacaac ttcgggaaaa tgaacacaat ttcacggtcg tttcccacca
     4801 caggaaaacc gtatccgaac accattccgc ccggtttgcg ccgttgccgc aagccggctg
     4861 ttttctgaaa aaccaacgca acaacccgcc ggaacaccgg cagcctttaa aggaacagaa
     4921 atggatttgc gcaaattaaa aaaactgatt gatttggttg aagaatcggg tatcgccgaa
     4981 atcgaagtaa ccgaaggcga ggaaaaagtc cgcattaccc gtaccattgc agctgcggca
     5041 gctcccgttt acgccgcgcc cgtacctgcc gccgcgccgg ccgtaacgcc ggccgcccgc
     5101 gatttgtccg acgctcaaaa atcgcctatg gtcggcacgt tctaccgcgc gcccggcccg
     5161 aatgccgccg catttgtcga agtcggtcaa caggtcaaag ccggcgacac gctgtgcatc
     5221 atcgaagcga tgaagctgat gaacgaaatc gaagccgaaa aatccggcac ggtcaaagaa
     5281 attctggtcg aaaacggtac gcccgtcgaa ttcggcgaac cgctcttcat catcggataa
     5341 tcctgttttc agacggcata aacttccgat gccgtctgaa atgctttccc cccttcagcg
     5401 ttcccgcacc ctttttttac ggacgggttg ccggaaccgc aggaaaggtc atcatgctga
     5461 aaaaagtttt aatcgccaac cggggcgaaa tcgccttgcg cgtactccgt gcctgccgcg
     5521 aaatgggcat tgccaccgtt gccgtgcatt ccgaagccga caaaggcagc ctgcacgtca
     5581 aactcgccga cgaatccgtg tgcatcggtc ctgccgcttc tgcgcaaagt tatctgaaca
     5641 ttcccgccat cattgccgcc gccgaagtaa cctgcgcgga cgctgtccac ccgggctacg
     5701 gcttccttgc cgaaaacgcc gatttcgccg aacaggtcga acagtccggc tttaccttca
     5761 tcggcccgaa acccgacacc atccgcctga tgggcgacaa agtctccgcc aaacacgcga
     5821 tgatagcggc aggcgtgccc tgcgtccccg gctctgacgg cgcattgccc gacgatgatg
     5881 ccgaaatcct caaaatcgcc gacaaagtcg gttatcccgt gattatcaaa gcatcgggcg
     5941 gcggcggcgg gcgcggtatg cgcgtggtcg agaaaaaaga agacctcctc cagtctgtcg
     6001 aaatgaccaa ggctgaagcg ggcgcggcgt tcggcaaccc gatggtttat atggaacgct
     6061 acctgcaacg cccgcgccac gtcgaaatcc aagtgcttgc cgacgaacac ggcaacgccg
     6121 tctatcttgc cgagcgcgac tgttcgctgc aacgccgcca ccagaaagtc atcgaggaag
     6181 cacccgcccc gtttatcgat gaagaggcac gcaaaaaaat cggcaaagcc tgtaccgacg
     6241 cgtgcaaacg catcggctac cggggcgcgg gcacgtttga atttttatac gaagacggcg
     6301 aatttttctt tatcgagatg aacacgcgcg ttcaggtcga gcatccggtt accgaactca
     6361 ttaccggcgt ggacatcgtc caagagcaac tccgcatcgc atccggcctg ccgctgcaat
     6421 acaaacagaa agacatcaaa atcgaaggcc acgccttcga gtgccgcatc aatgccgaag
     6481 acccgtacaa cttcatcccc agcccgggcc cgattgaaag ctgccacctg cccggcggct
     6541 tcggcatccg cgtggacagc cacatctacc aaggctaccg catcccgccg tactacgaca
     6601 gcctgatcgg caaaatctgc gtacacggca aaacgcgtga acaggcaatg gcgaaaatgc
     6661 gcgtcgcact cgccgagctg gcggtaaccg gcatcaaaac caatacgccg ttgcaccgcg
     6721 acctgttcgc cgatgcgggt ttccaagaag gcggagtgag catccattac ctggagcact
     6781 ggctggaagc gcgcaaaacc aaacaggaca aataagccgc cgccgatatg ccgtctgaag
     6841 ccgcccttcc gcgttcagac ggcatttccc ttgccccgcc gtctgaaacc gatttcgata
     6901 tttccaagaa agcccgttat gccctaccaa caaatcaccg tcaacgtcaa cgatgccgtc
     6961 gccgaacgcc tcgccgacgc gctgatggaa cacggtgcac tctccgccgc catcgaagat
     7021 gcctgcgccg gcacgcaaaa cgaacaggcg attttcggcg aacccggtat gcccaccgaa
     7081 caaatctggc agcagagcaa agtcatcgcc ctgttcggcg aacacgacga agccgccgcc
     7141 gtcatcgatg ccgccgcaca agaatgcggg ttaaaagact tggcatacac cggcgaaacc
     7201 atcgaagacc aagactgggt gcgcctcacg caatcgcaat tcgaccccat ccgaatttcc
     7261 gaccgcctgt ggattacccc ctcttggcac gaagcccccg aaggctgcgc cgtcaacctc
     7321 cgcctcgatc ccggactcgc cttcggcacc ggcagccacc cgaccacgcg cctctgcctc
     7381 aaatggctgg atacgcaact caaaaacggc gaaagcgtcc tcgactacgg ctgcggttcg
     7441 ggcatcctga ccatcgccgc cctcaaactc ggtgcaggtt ccgccgtcgg cgtggatatt
     7501 gacgaacagg ccgtccgctc cggcagggac aacgccgaac agaacaacgt cgatgcacaa
     7561 ttcttcctgc ccgacagtct gcctcaaggg caattcgacg tagttgtcgc caacatcctc
     7621 gccaaccctt tgcgtatgct cggcgaaatg cttgcagccc gcaccaaaca gggcggacgc
     7681 atcgtgttgt ccggtttgtt ggatgaacag gccgaagaac tcggcggcat ttacagccaa
     7741 tggttcgatc tcgacccggc ggaaactgac gaaggctggg cgcgcttgaa cggcgtaaaa
     7801 cgctgaaacg gaaaggaaac accgtgcagg acaaaaacaa cctctgctgg ctcgatatgg
     7861 aaatgacggg gctgaatccc gaaaccgacc gcattatcga agtcgcgatg attattaccg
     7921 actcggattt gaatgtgttg gcgcaatccg aagtttacgc catccaccaa agcgacgatc
     7981 tgctcgacaa tatggacgag tggaacaccg ccacacacgg caggacgggg ctgacgcagc
     8041 gcgtgcgcga atcgtcgcat accgaagccg aagtcgaaca gaaactgctg gactttatgt
     8101 cggaatggat acccggacgc gccacaccga tgtgcggcaa ctccatccac caagaccggc
     8161 gttttatggt caaatatatg ccgaaactgg aaaactactt ccactaccgc aacctcgacg
     8221 tttccacgct gaaagaactc gccaaacgct ggaatccgcc cattgccaaa agcgtcgtca
     8281 aacgcggttc gcacaaggca ttggacgaca ttttggagag catcgaagaa atgcgccact
     8341 accgcgaaca cttcctgatt tccgccccga aagccgaagc gcaataagaa acaaacaatg
     8401 ccgtctgaaa cgctat
//
LOCUS       DAXFZR010000085         8283 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000085 DAXFZR010000000
VERSION     DAXFZR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.086"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(117..416)
                     /locus_tag="ACFJAS_001817"
     CDS             complement(117..416)
                     /locus_tag="ACFJAS_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3982990.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERYGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFILARQGHGVVVAMQ"
     gene            complement(416..1717)
                     /gene="nuoF"
                     /locus_tag="ACFJAS_001818"
     CDS             complement(416..1717)
                     /gene="nuoF"
                     /locus_tag="ACFJAS_001818"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC3982991.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            complement(2107..2580)
                     /gene="nuoE"
                     /locus_tag="ACFJAS_001819"
     CDS             complement(2107..2580)
                     /gene="nuoE"
                     /locus_tag="ACFJAS_001819"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC3982992.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(2580..3836)
                     /gene="nuoD"
                     /locus_tag="ACFJAS_001820"
     CDS             complement(2580..3836)
                     /gene="nuoD"
                     /locus_tag="ACFJAS_001820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC3982993.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRIILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(3826..4419)
                     /locus_tag="ACFJAS_001821"
     CDS             complement(3826..4419)
                     /locus_tag="ACFJAS_001821"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC3982994.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(4432..4914)
                     /locus_tag="ACFJAS_001822"
     CDS             complement(4432..4914)
                     /locus_tag="ACFJAS_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC3982995.1"
                     /translation="MGIEGVLKKGFITTSADTVLNHMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(4905..5261)
                     /locus_tag="ACFJAS_001823"
     CDS             complement(4905..5261)
                     /locus_tag="ACFJAS_001823"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC3982996.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            complement(5754..7205)
                     /locus_tag="ACFJAS_001824"
     CDS             complement(5754..7205)
                     /locus_tag="ACFJAS_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFC3982997.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTPFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIAGRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDIFNSVNGIERAYLPPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIRMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(7228..7848)
                     /locus_tag="ACFJAS_001825"
     CDS             complement(7228..7848)
                     /locus_tag="ACFJAS_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC3982998.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
     gene            complement(8047..8283)
                     /locus_tag="ACFJAS_001826"
                     /pseudo
     CDS             complement(8047..8283)
                     /locus_tag="ACFJAS_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2171 a         2066 c         2058 g         1988 t
ORIGIN      
        1 catttattat atttttaaat gacttaaata actctttaac agcacgctca ttgtcttcat
       61 ctgtaggctc aaacttttca caccaattaa ttatatttac atcgccatgc aatatcctac
      121 tgcatcgcaa ctaccacccc gtgtccttga cgtgccaaaa taaaatcaca attatgcgaa
      181 taaagtttgt ataaatttct cctaacctcg tattcattat caccagatga ataaaaacca
      241 atcctctgac cttcatgatc aaatactgca acaaaatcta agccgtttcc atatctttca
      301 aaaaagacta gaaatttaga aaatttccga caaatctgat taaaaagcgt attgagtgtc
      361 gtactcttac ctttgttggc tgcaccgtat agaataaaaa ttttagcttc caccattacc
      421 accctcccca cttatgctct ttcatcggcc cgccgtgttc gatgtaatgc gcaaactcat
      481 cacggaaatg cttggtaaag ctgcggacag ggaaaacggc ggcatcggca agggcgcaga
      541 tggtgcggcc tgccatttgg ttgccgacag aatccagcaa atccaagtct tccatgcggc
      601 ctttgccttc tacgatgcgg cggacgatgc ggtaaagcca gccggtacct tcgcggcagg
      661 gggtgcattg gccgcaggat tcgtcgtagt agaaatagct taagcgttcg agggctttga
      721 ccatgcacac gtcttcgtcc atcacgataa tcgcgccgga accgagcatc gagccggctt
      781 tggaaatcga gtcatagtcc atattggtct gcatcataat gtcggcaggc aatacggggg
      841 cggacgaacc gccgggaata acggctttga gttttttacc gccgcgcata ccgcccgcca
      901 ttttcaagac ttcggcaaac ggcgtgccca acggcacttc atagttgccc ggacgctcga
      961 catggccgga aatacaaaat aatttggtac cgcctgcatt cggaatacct ttatcggcaa
     1021 acgcctgtcc gccgtcacgg ataataaacg ggacggagga gaacgtttcg gtattgttga
     1081 tggtggtcgg tttgccgtac aggccgaacg aggcggggaa cggcggtttg aagcgcggct
     1141 ggcctttttt gccttccaac gattcaagca atgcggtttc ttcgccgcaa atatatgcgc
     1201 cgtagccgtg gtgggcgaag agttcgaatt caaaatccga acccaaaata tttttaccca
     1261 aaaagcctgc ggcacgcgcc tgctccaaag cggcttcaaa gcgttgataa ccttcgaaaa
     1321 tttcgccgtg gatatagttg tagccggctt tcgcgcccat cgcgtaaccg gcaatgatca
     1381 tgccttcaat cagggcatga ggattgaaca tgatgatgtc gcggtcttta aatgtacccg
     1441 gctcgccttc gtcggtgttg caaaccacat atttttcgcc cgggaaagaa cggggcataa
     1501 agctccattt caaaccggtc gggaagcccg caccgccgcg cccgcgcaag ccggaggttt
     1561 tgacttcgtc aatcacatcg gtttgcgaga tgttttcgga caggatttta cgcagggcgg
     1621 tatagccgcc gcgtttgacg tattcgtcca atgtccagca atcgggattg gcggtatcca
     1681 cttggtcaaa aatcacgcct gattggtaaa tagccatttt tggtgtgcct gtttgttttc
     1741 gtatcggttg cggccgctgt ttcagacgac cttaagatgt ctttgtgtat cggcttgtaa
     1801 cgtcttctga aataaaatct agtttatcaa atcgatattt tcagacgacc ttcaatcgtt
     1861 tttgttggat ctcgaccact tgcttgtctg ccgtcattcc cgcgcaggcg ggaatccatc
     1921 ctcaatggta agcaatgtct tattaaattc agaaaccgaa tcttaccggt ggattcccgc
     1981 ctgcgcggga atgacggcat ttcagtattt cagtagggcg gattcttaaa tccgacattt
     2041 tgccttttta cccactctgt cggatacaag tatccgacct acgtttaaat cgtcgtttca
     2101 gacgacctac tccaactccg ccagtttctt ctcaatcgcc tcttcggtca taaagctgca
     2161 catgctgtgg ttgttgacca gcataacggg ggcatcgccg cacgcgccca tgcattcgcc
     2221 ttcgacaagg gtaaatttgc cgtcgggcgt ggtttcgccg tagccgatac cgagtttttg
     2281 tttgaggtat tcgccggtcg ccataccgcc gcgcagagcg cagggcaggt tggtacaaac
     2341 ggtcagtttg tatttgccga caggctcaag gtcgtacata ttgtagaaag tggcgacttc
     2401 atatgcctgc gccggcgtga tgccgatgta gtcggcaaca aaagcgatgg tttcgggggc
     2461 gagccagcct ttttcggttt gggcgatacg caacgcgccc atgatggcgg aacggcgttg
     2521 gtcggcggga tatttcgcca actcaatgtc gatttgtttt aaggattttg cggataacat
     2581 tatcggtcaa cctccccgaa tacgatgtcc tgcgtaccga tgatggcaac gacgtcggcg
     2641 agcatatggc cttttgccat ttcgtccatg ccttgcaggt gggcgaagcc gggtgcgcgg
     2701 attttcaggc ggtagggttt gtttgcgccg tctgaaatga tgtaaacgcc gaactcgcct
     2761 ttcgggtgtt cgacagcggt gtaggtctcg ccctcgggaa cgtgcatacc ctcggtaaag
     2821 agtttgaaat ggtgaatcag gtcttccata cctgttttca tttcggtacg tttgggcgga
     2881 gcgaatttgt ggtttgcggt aatgaccggg ccgggattga cgcgcaacca gtcggcgcat
     2941 tgtttgatga tgcgtacgga ttggcgcatt tcttccatac ggcagaggta acggtcgtag
     3001 cagtcgccgt tgacgccgac ggggatgtcg aaatccattt tgtcgtacac ttcgtagggc
     3061 tgtgtcttac gcacgtccca ttccacgccg gaaccgcgaa gcatcacacc ggtaaagcct
     3121 ttttgcatgg cgcgctcggg agagacaacg ccgatgccga cggtacgctg tttccagatg
     3181 cggttgtcgg tcaggagggt ttcgagtgtg tcgatatttt tggggaagcg ttcgcagaag
     3241 gcatcgataa agtcaagcat cgtgccttcg cgggattcgt tgagctgctt caatactttg
     3301 gcattgcgga atttgctgct ctcgtatttg ggcataaagc cgggtaggtc gcggtaaacg
     3361 ccgccgggac ggaagtaggc ggcgtgcata cgtgcgccgg acacggcttc gtacaagtcc
     3421 atcagctctt cgcggtcgcg gaaggcgtag aggatggcgg tcatcgcgcc gatgtcgaag
     3481 gcgtgcgaac cgatgcccat caagtgattg aggatgcgcg ttacttcggc gaacatcacg
     3541 cggatgtatt gggcgcggat aggcacatcg ataccggcaa gtttttctac cgccaagcaa
     3601 tacgcctgct cgttgaccat catggaaacg tagtccaaac ggtccatata gggcagggct
     3661 tgcagatagg ttttggtttc cgccagtttt tcggtgcccc ggtgcaggag gccgatatgc
     3721 ggatcggcac ggacgatttg ttcgccgtcc agctccaaaa ttatacgcaa tacgccatgc
     3781 gccgcagggt gctgcgggcc gaagttgatg gtgtagtttc ttaatttatt ggccaccgta
     3841 gttctcctca cggacgatac gcggtgtgat ttcgcgcggc tcaatggtaa caggttggta
     3901 aatcacgcgt ttttgctctt cgtcataacg catttccaca tagccggaaa tcgggaagtc
     3961 tttgcggaac ggatgtccga caaagccgta atcggtcagg atgcggcgca agtcgggatg
     4021 gttgttgaac atgatgccgt acaaatcgaa ggcttcgcgc tcgtaccaat ccgcgctgtt
     4081 gtaaatatcg gctacggatt cgactacagg gaagtcgtcg tctgaaaccc agacgcgtac
     4141 gcggatgcgt tgattgtttt taacggaaag caactgactg acaacggcaa agcgtttgcc
     4201 ctgccatgct tcgtttttgt aagtgctgta atcgacgccg cacaagtcaa ccagaagctc
     4261 gaaatgcaaa tcttcatggt cgtgcaatgc ggtcatgact gaaatatagt gctcgggcag
     4321 acactcgacg gtaatctcgc ccaaagctga aatgactttg cctgcctgat cgccaagcac
     4381 gccgacgacg gtctcgtata agttttgaat gcttgccata tcgtcctctc cttactcgtc
     4441 acgcgcgatg gtggaagtgc gcttgatttt ttgttggagc tgaatcaggc cgtaaatcag
     4501 agcttccgca gtcggcggac aacccggcac ataaacgtcc accggcacga cacggtcggc
     4561 accgcgcaca acggaataag aatagtgata atagccgccg ccgttggcac acgaacccat
     4621 agacaatacc cagcgcggct cggcgagctg gtcgtacacg cgacgcaggg ctggcgccat
     4681 tttattggtc agcgtacctg ccacaatcat caggtcggcc tgacgtgggg acggacggaa
     4741 aatgataccg aaacggtcaa ggtcataacg cgccataccc gcatgcatca tttccacggc
     4801 gcagcaggcc aatccgaaag taaccggcca caatgaaccg gtacgcatat ggttcaacac
     4861 cgtatccgcg ctggtggtga tgaaaccttt tttcaaaacg ccttctattc ccattccagc
     4921 gcaccttttt tccattcgta aacaaagcct accgtcagga cgacgataaa caccagcata
     4981 gaccaaaagc cgtacgcgcc caaatctttg aacacgactg cccacggcag cataaacgca
     5041 acctccaaat cgaagaggat gaacaggatg gcgacgaggt aatagcgcac gtcgaacttc
     5101 atccttgcgt tttcaaaggc ttcaaaaccg cattcgtaag gcgcgtcttt ttcggcatag
     5161 tggcgtttcg ggcccaaaat cgtgccgagc aggataaaca gtacgccggc cgcgaggccg
     5221 acgaggataa agacaaagac gggaaagtaa gcggacaaca tggttacacc caaatccgtt
     5281 aacaaaattt ctacaataat ttcgtatttt agcgaatttc aaaaaccatt aaaaggtaaa
     5341 tatcggcaaa acgcccaaaa aaacccaata aatacaatca tgttatgata acgattctta
     5401 tttgatttta taaggtcata taaattttta caccgtaatt cgtggtatag cggattaaca
     5461 aaaaccggta cggcgttgcc ccgccttagc tcaaggggaa cgattcccta aggtgcccaa
     5521 gcaccaagcg aaccggttcc gtactatttg tactgcctgc ggcccgccgc cttgtcctga
     5581 tttttgttaa tccgctatat ataggaataa gaattcggga caaaatgttt aaaccatttt
     5641 gtcccgactg ctgtgatgcg gtttttttga actaacagga cgttacctaa aacctgcgtt
     5701 acctaaaacc tgcgttgcct aaaacctgcg ttgcctaaaa ccctttaaga cggttaaatc
     5761 cctctgccgt atttgtattc tacaatcata ttatcgtcag taatgacttc cgccccagcc
     5821 gaaggttccg tcatccgaat cagcatacga gagacaacct tttgtgctgc agcatccacg
     5881 gtgctgctgt caaatacgtg cctgccgctt tccggccaaa tcaaccggga aaggcgttgc
     5941 ttgagcagtt ctttattagg gaaaactacc ggggttgccg agccgactac catatgcccg
     6001 tagcggtatg catagggaat actgtgtacg gcggtagcaa aagcatgcgg gctgtgcgtg
     6061 gtattaaaca ttacaatacc atccggggta aggtggcttt gcacctgttt taaaaattcc
     6121 gcactcaaca ggttagtgga ataggcacgc cagtaccaag tcgaattcat caaaatcagg
     6181 tcgaattttt catcaggatg gcgacgcagc catttcctac cgtcatccaa tacaatttca
     6241 acacgtttgt cctgcaaaag cggtgcgatt tgcggctcgt ccgcgataag gctacggtat
     6301 gccggattga tttccgcaac gatcatcgac tgcatttccg gaatggcaga caagacgcgc
     6361 gcccacgaac ctgtactcaa tccaacgacg aaaatgcggc gtatgccgga cttcagggag
     6421 ggtggcagat aggcacgttc gatgccgttg acactattga atatatcggt attgtatgcg
     6481 ccgtcgtata cattcgcccc ataaacaacc ttatcaccat ctctatggta aaccgcaaca
     6541 atgccgtgtt tgttttcaat caacctatcc ggacggccag caatattttg aaagacggaa
     6601 tccggcagta ggaacatgag gatgccgaac attagggaaa ctgctaccga cactgcattc
     6661 agtcggagac ttttttggaa cggtgtacaa aacaaaggga cagcagcaga aatcaaacag
     6721 atgagcaggt aaatctgttg ggtggacaag aaatcaagta tcacaaagcc gataaggacc
     6781 ggacccaatg cactgccggc aacgttggcg aaataaacat tggaaacctg tcgtccggat
     6841 ttgttgccat ccgtacccac atggtgtaca agtgggaaaa tcaaccccct gacgacggca
     6901 gacagggtaa tgaagatacc ggcgtggtgg acgaagccgg aaaaacccgt caacaaccac
     6961 gcagcaccca aaatcaaaaa atcggcaata cccgcccaca agaagcactg cccgataaag
     7021 ggaatatcaa caaagcggct gcggcaaatc cgtttgccaa aatacgcgcc gacggcgata
     7081 ccggtcagaa aacaggcaag agtaaatgaa aatgcctgag gcacggactg tgctgcgaac
     7141 gaaaacatcc ttacccacaa gacttctata cccaagctca ataatccgct aaggaaagaa
     7201 agcatataaa tcaaactagt attcggttta gtgttcacca tatccatcct ttctgtaacg
     7261 cagccatact gaagcagcaa tcagaaggtt aaggcaggct gtcagcgcaa tggtttggga
     7321 gagggtaaaa aagacgtaga aaaattcggc ggcggcaagc gatccgagtg ccgcacccaa
     7381 agtgttgaaa aaatataagg taccgataga ctcgccaaca ttatgtattt tccggttaaa
     7441 aaaacaggtc agcaagggca aggtcgcgcc catcataaag gtaggaagca gcaataagag
     7501 gaaattggca gcagcgatga tgggcaaatc agcctcaact aaaagatgcc ccaagccgga
     7561 aatcagaccc ttgcttacca aaccgaacag accgatggat acttcagcga tgcaaaacag
     7621 ggggatgata cttgaaggaa aacggtcagc aatgcgtccg ccgaaatacg cacctacacc
     7681 caagccgacc ataaatacag aaataatgac agtaatcgaa ctcaaatcga tacctatgtg
     7741 gctgaataga agcctctgcc agctgacctg gtaaatcagg gcgcagaagc cagaggcgaa
     7801 aaacaccagc gacaatcctt tcagggtttt acttgcggtc gaattcatat tttctatttt
     7861 caaataggcg gaactataaa tgaaatgtat taatgtgtaa acatttgtat tagccgtagg
     7921 aaatcaccgc attgctcaaa ctgcaggccg ggaaaataaa ctcgtaagcc cgcaaacggc
     7981 gtatcactgt tcaaactgct gtgcggcttc atgacgttat aaaaattaag gggccgtcct
     8041 ggacaactag gataaactcg attttactaa ttgttttaaa atggaacaag aacttttatc
     8101 tcactgttgt taaaacgccg ttcgcactcc tttaaatacg gctcaaaatg ctctttggga
     8161 atgccgtcaa acttgcgtaa atgacgtttt gcccggttcc aaaagttccc aattccatta
     8221 atatggtttt gtcgttcggc aaaatgtgtg ctgtgattga tacggaaatg gctaaattcg
     8281 ccc
//
LOCUS       DAXFZR010000086         8126 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000086 DAXFZR010000000
VERSION     DAXFZR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.087"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1309
                     /gene="gshA"
                     /locus_tag="ACFJAS_001827"
     CDS             <1..1309
                     /gene="gshA"
                     /locus_tag="ACFJAS_001827"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC3982999.1"
                     /translation="QAFEAKILSNHAKIEAWFRTQWNAHRPPFYGSVDIRNAGYKISS
                     IDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPENHTRNTFYLQNVYA
                     LGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTRDRVHLADGFSPCVV
                     LLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQVAAEFAKLIDIDEWQ
                     INPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIADKPFVIVKADAGTYG
                     MGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTYETLNGAVCEPVVYM
                     MDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNSQEAPEACKRVFEQW
                     DSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            complement(1283..1405)
                     /locus_tag="ACFJAS_001828"
     CDS             complement(1283..1405)
                     /locus_tag="ACFJAS_001828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983000.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            1432..2109
                     /gene="radC"
                     /locus_tag="ACFJAS_001829"
     CDS             1432..2109
                     /gene="radC"
                     /locus_tag="ACFJAS_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC3983001.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            2182..2907
                     /locus_tag="ACFJAS_001830"
     CDS             2182..2907
                     /locus_tag="ACFJAS_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC3983002.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            3059..4339
                     /locus_tag="ACFJAS_001831"
     CDS             3059..4339
                     /locus_tag="ACFJAS_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC3983003.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDALMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRHAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            4345..5523
                     /gene="hflX"
                     /locus_tag="ACFJAS_001832"
     CDS             4345..5523
                     /gene="hflX"
                     /locus_tag="ACFJAS_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC3983004.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            5520..7991
                     /locus_tag="ACFJAS_001833"
     CDS             5520..7991
                     /locus_tag="ACFJAS_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC3983005.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDREEAVAETARALGIAHYRA
                     QAMPEDKLEYVEALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
BASE COUNT         1972 a         2334 c         2227 g         1593 t
ORIGIN      
        1 tcaggcgttt gaggcaaaaa tcctgtccaa tcacgccaaa atcgaggcgt ggttccgcac
       61 gcagtggaac gcgcaccgcc cgccgtttta cggttcggtc gatatacgca acgccggtta
      121 caaaatttcg tctatcgata tgaatttgtt tccgggcggc ttcaataatc tgaatccgaa
      181 tttcatcccg ctggcggcgg ttgccgcgca agatgcggtg caacgcgcct gcgaaacgga
      241 gaaatccgta ttgattattc ccgaaaacca cacgcgcaat acgttttacc tgcaaaacgt
      301 ttacgccctc ggcgagattt tccgttcggc agggtgtgaa gtgcgcttgg gcagcctgaa
      361 tccggaagta accgaaccgg ccgaatttga aactgcattg ggcgacaaaa tcctgcttga
      421 gcctttgctg cgaacccgcg accgcgtcca ccttgccgac ggcttttcgc cttgcgtggt
      481 tttgttgaac aacgatttgt ccgccggcat tcccgacatt ctcaaaggca tcggccaaac
      541 cgttttgccg ccgctgcacg gcggttggac gacgcgccgc aaaacaaatc atttcggcgc
      601 gtacaaccaa gttgccgccg aatttgccaa gctgattgac atcgatgaat ggcagatcaa
      661 cccgtatttt gaaaaaatcg gcggtttgga cttccaaggg cgcgaaggtg aagatgcgct
      721 ggcggaagcg gtagaacgcg tgctggcgaa aattcaagcc aaatacgacg aatcgggcat
      781 tgccgacaaa cctttcgtca tcgtcaaagc cgatgccggc acttacggca tgggtgtgat
      841 gagcgtcaaa tcctccgacg aagtgcgcgg cttgaaccgc aaaaaccgca acaaaatggc
      901 gaaggtgaaa gagggcttgg aagtcagcga agtgattgtc caagaagggg tttataccta
      961 tgaaaccttg aacggcgcgg tgtgcgaacc cgtcgtctat atgatggacc gtttcgtcat
     1021 cggcggcttt ttccgcgtac acgaagggcg cggtgcggac gaaaacctca acgccggcgg
     1081 tatggtgttt gtgccgctgt ccaacagcat tcctaccggt aacggcgata attcgcaaga
     1141 agctccagaa gcctgcaaac gcgtattcga acaatgggat tcgctcggta tgccgcgttc
     1201 tgaaaaagac tgcgacgtgg acaacgaaca taaccgcctc tacgtttacg gcgtaatggc
     1261 acgtctgtcg cttttggcgg cttcaatcga gttggaagaa acggcgtaag actgttttga
     1321 aatacagatg ccgtctgaag cggaaatccg gttcagacgg catttcggat atttggcgtg
     1381 tgggaacatc tgtttcagac ggcatctcag actatttaaa aaagggaaaa catgagcatc
     1441 aagcaatggc cggaaggcga aagacccagg gaaaagctgt tggaacgcgg ggcggcggct
     1501 ttgagcgatg ccgaactttt ggcaatcctt ttacgcgtcg ggacgcgcgg gatgagcgcg
     1561 gtcgatttgg cgcgctacct attgcaggag ttcggcagct tggggaggct gatgagcgcg
     1621 gaggtcggca aactgtcggc atacaaaggg atggggacgg caagtttcac acagttcgcc
     1681 gtggtcaggg aaatcgggcg gcggatattg gaggaagaat tgcaggaaga aattacctta
     1741 tccgatcccg ataccgttgc cgattattta cgctttcatt tggggcagga aaaagtcgaa
     1801 gtcagcgtcg cgctgctgct gaaccgccaa aaccaactga ttgcggtcag agagctgtcg
     1861 cgcggtacgg ttgcggaaaa cacgatttac atccgcgaaa tcgtcaaact ggcattggac
     1921 gaatatgccg acagcctgat tatcgcgcac aaccatccgg gcggctcgcc cgaaccttcg
     1981 caggaagaca tcatgttcac aaggcggctg gcacaggcca tgtcgctggt tgatgtttcg
     2041 ctgctcgacc attttatcgt tacctcgcaa accgtccgtt cgttcaggca gctcgggctg
     2101 atgccctgac accctgtttt acattcggcg gctctggtaa aatagccgct ttgaccgtat
     2161 tcgacagata ttgttaagtt aatggaaaca caaaacaaac ctaccgttac cgacattgac
     2221 cgccccatac tcgtcccgcc cggcggacat aaaaaagtct tgctgcattc ctgctgcgcc
     2281 ccgtgcagcg gcgaagtgat ggaagccatg cttgcctccg gcatcgacta caccatttat
     2341 ttttacaatc ccaatatcca tccgcacaaa gagtatatgc tccgaaaaga ggaaaacatg
     2401 cgctttgcgg aaaagttcgg catccctttc atcgataaag acgacgacta cgaaaacgac
     2461 cgcaaagaat ggtttgccaa agccaaaggc atggagtttg agccggaacg cggcatccgc
     2521 tgcaccatgt gtttcgatat gcgttttgaa aaagcggcgc aatacgcgca cgaacacggt
     2581 tttcccgtct ttaccagttc gctgggcatt tcacgctgga aaaatatggc gcaaatcaac
     2641 gactgcggac accgtgccgc cgcgccttac gatgatgtgg cctattggga cttcaactgg
     2701 cgcaaaggcg gcggcggcgc gcgtatgatt gaaatcagca aacgcgaaaa cttctaccag
     2761 caggaatact gcggctgcgc ctattccttg cgcgattcca acgcccaccg caaatcgcag
     2821 ggacgcatcc ccgtcaaact cggcgtgctg tattacggcg acgaatcgac acaatacgaa
     2881 cctgcacccg tccgagtgga caaataaaca cccgatgccg tctgaaggtt cagacggcat
     2941 cgggttcggc accggcacgg gaaaggtttg ccggtttggc aatcggcaag cggaaaccgc
     3001 attggcaagt ttgccgtttt gataaaacac cccgttgccg cgtcgggagg gcggcattat
     3061 gaaatccctt tttatttggc tgcttctatt gggctcggcg gcaggcgttt tctaccatac
     3121 ccaaaaccaa tccctgcccg cgggcgaact tgtctatccg tccgcaccgc aaatcaggga
     3181 cggcggcgat gcgctgcact acctcaaccg catccgcaca caaatcggtt tgcacgcgct
     3241 ggcacacgcg ccggttttgg aaaattccgc ccgcaggcac gcacgctatc tcacgctcaa
     3301 tcccgaagac ggacacggcg aacaccatcc cgacaatccg cactacaccg cacaaaagct
     3361 gaccgaacgc acacgccttg ccgggtatct ctacaacggc gtgcatgaaa acatcagcac
     3421 ggaagaggaa gccgccgaat cgtccgacag cgacatccgc acgcagcaac gccaagtgga
     3481 cgctttgatg agcgcaatct accaccgcct ttcgctgctt gaccgccata ccgacgaagc
     3541 aggtgcggca tttgtgcgcg aaaacggcaa aaccgtcctc gtattcaatc agggcaacgg
     3601 cagcttcgag cgcgcctgtg caaaaggaag gcggcagccg gaagcaggac ggaaatatta
     3661 ccgcaacgct tgccacaacg gtgcggccgt ttatgctgac gaagccatgc ccgtaacgga
     3721 attgctttat accgcctatc cggttggcgg cggcgcgctg ccttattttt acggggaacg
     3781 tcccgacccc gtgccggaat atgaaatcac aggcaatcct gccagcattg atttttccga
     3841 ggcggcaggc aaaattgcga tgaaaagttt caagctgtat cagggtaaaa acgaaatccg
     3901 ccccgtcagg gttttaaccg ccggcaacga ccctaacggc aggctgaccg cgcaccaatt
     3961 cgcccttttc ccgctcaaac ctttggaata cggcacgctt tatacggcgg tattcgacta
     4021 tgtccgcaac ggacggcacg cgcaggcgaa atggcagttt agaacccgaa aacccgatta
     4081 cccttatttt gaggtaaacg gcggcgagac acttgcggtt agaaaaggcg aaaaatattt
     4141 catccactgg cgcggacgct ggtgtctgga agcgtgtacc cgttatacct accggcggca
     4201 gttcggcaac agcctgtcca tactccggca cgaagcgggc ggcattgtct tcagcgtcag
     4261 cggaatggcg ggaagccgca tcaggcttac tccggaagac agcccggaac gcggtgtaac
     4321 cctttattta caggattgac agttatgtca ggtagaacag gacggaacag tgccactcag
     4381 gcgcaaccgg aacgcgtcat gctggtgggc gtaatgttgg ataaagatga tacgggcagc
     4441 aatgccgccc gtctgaacgg ttttcagacg gcattggcgg aagccgtcga gctggtcaaa
     4501 gcggcgggcg gcgattccgt acgcgtggag actgccaaac gcgaccgccc gcacactgcg
     4561 ctgtttgtcg gcacgggcaa ggcggcggag ctgtcggaag cagttgccgc agacggcatt
     4621 gatttggtcg tattcaacca cgaacttact cccacgcagg aacgcaattt ggaaaaaatc
     4681 ctccaatgcc gcgtattgga cagagtgggg ctgattctgg cgattttcgc ccgccgcgcc
     4741 cgcacgcagg aaggcaggct gcaagtcgag ttggcgcaat tgagccattt ggcgggacgc
     4801 ttgatacgcg gttacggaca tttgcaaagc cagcgcggcg gtatcggcat gaaagggccg
     4861 ggcgaaacca aactggaaac cgaccgccga ttaaccgccc atcggatcaa cgccttgaaa
     4921 aaacagcttg ccaacctcaa aaaacagcgc gccctgcgcc gcaagtcccg cgagtcgggc
     4981 agaatcaaaa cgtttgcgct ggtcggctat accaatgtcg gcaaatccag cctgttcaac
     5041 cggctgacca agtcgggcat atatgcgaaa gaccagcttt tcgccactct cgacacgacg
     5101 gcgcggcggc tgtacatcag tcccgcatgc agcattatcc tgaccgatac cgtcggattc
     5161 gtcagcgatc tgccgcacaa actgatttcg gcattttccg ccacgctgga agaaaccgcg
     5221 caagccgatg tgctgctgca cgtcgtcgat gccgctgccc cgaacagcgg acagcagatt
     5281 gaagacgtgg aaaacgtact gcaagaaatc catgcccacg atattccgtg catcaaggtg
     5341 tacaacaaaa ccgacctgct gccgtctgaa gaacaaaaca cgggcatatg gcgcgacgct
     5401 gcgggaaaaa ttgccgccgt ccgcatttcc gttgctgaaa ataccggtat agacgcactg
     5461 cgcgaagcca ttgccgagta ttgtgccgcc gcaccaaaca cagacgaaac cgaaatgcca
     5521 tgaaaaaaac ctgtttccac tgcgggctgg acgttcccga aaacctgcat ctgaccgtcc
     5581 gctacgaagg cgaagaccgc gaaacctgct gcgtcggctg tcaggcagtc gcacaaagca
     5641 tcatcgattc cggcttgggc agttattaca aacgacgcac cgccgacgcg aaaaaaaccg
     5701 aactgccgcc ccaagaaatc ctcgaccaaa tccgcctgta cgacctgccc gaagtccaat
     5761 ccgactttgt ggaaacccat aatggcacgc acgaagcagt gctgatgctc agcggcatta
     5821 cctgcgccgc ctgcgtctgg ctgatcgaac agcagctttt gcgcacagac ggcatcgtcc
     5881 gcatcgacct caattacagc acgcaccgct gccgcgtcgt ctgggacgac ggcaaaatcc
     5941 gcctctccga cattctgttg aaaatccggc agacaggcta caccgccgca ccctatgacg
     6001 cgcaaaaaat cgaagccgcc aaccaaaaag aacgcaaaca atacatcgtc cgccttgccg
     6061 ttgccggact gggtatgatg cagacgatga tgttcgcgct gccgacctac ctttacggcg
     6121 gcgacatcga acccgatttc ctgcagatcc tccattgggg cggcttcctg atggtgctgc
     6181 ccgtcgtatt ttattgcgcc gtcccgtttt atcaaggcgc gttgcgcgac ctgaaaaacc
     6241 gccgggtcgg tatggacacg ccgattgccg ccgccatcat catgaccttc atcgccggca
     6301 tttacagcct cgccaccaac gcagggcagg ggatgtattt cgaatccatc gcgatgctgc
     6361 tgtttttcct gctgggcgga cgctttatgg aacacattgc ccgacgcaag gcaggcgatg
     6421 ccgccgaaag gctggtgaaa ctgatacccg ccttctgcca ccggatgccc ggctatcctg
     6481 ccgtacagga tgtccgcgaa tccgccgtcg tcaaactcca agcaggcgat attgtgatgg
     6541 tcaaacccgg cgaaaccatc cccgttgacg gcacggtgct ggaaggaaac agtgccgtca
     6601 acgaatctat gctgaccggc gagagcctgc ccgtcgccaa aatgccgtct gaaaaagtaa
     6661 ccgcaggtac actcaacacg caaagccctc tgattatacg caccgaccgc accggcggcg
     6721 gcacgcgact gtcgcacatc gtccgcctgc tcgaccgcgc cttggcgcaa aaaccgcgta
     6781 ctgccgagtt ggcggaacaa tacgcctcgt ctttcatatt cggcgaactc ctgcttgccg
     6841 tccccgtctt catcggctgg acgctgtacg ccgatgcgca caccgcattg tggattaccg
     6901 tcgccctgct ggtcattacc tgcccctgcg ccctgtcgct tgccacgccg accgcgctgg
     6961 cagcttctac cggtacgctg gcgcgcgaag gtattttaat cggcggaaag caggcaatcg
     7021 aaaccctctc ccaaaccacc gacatcattt tcgacaaaac cggcacgctg acccaaggca
     7081 atcccgccgt ccgccggatt gaactgctcg gcagcatgac cgaagcccaa gtgctggcag
     7141 tggcgcaaag tttggaacag cagtccgaac acccgctcgc gcgcgccatt ctcaactgcc
     7201 gcatttcagg cggcagcgtc cccgaaatcc aagtcggaca acgcctcaac cgcatcgggg
     7261 aaggcgtggg tgcgcaactg accgtcaacg gcgaaacaca ggtttgggca ttggggaggg
     7321 catcctatgt tgccgaaatt tcaggtaaag aaccgcaaac agaaggcggc ggcagcgcgg
     7381 tttacctcgg cagtcaaagc ggtttccaag ccgtgttcta cctgcaagac ccgctcaaag
     7441 acagcgcggc ggaggcagtg cggcagttgg caggcaaaaa cctgacgctg cacatcctca
     7501 gtggcgaccg tgaagaagca gttgctgaaa ccgcccgtgc cttgggtatc gcgcactacc
     7561 gcgcccaagc aatgccggaa gacaagctgg aatacgtcga agccttgcaa aaagaaggga
     7621 aaaaagtgct gatgataggc gacggcatca acgacgcgcc cgttttggcg caggcagacg
     7681 tatccgccgc cgcagcgggc gggacggata ttgcgaggga cggcgcggac attgtgttat
     7741 tgaacgaaga tttgcgtacc gtcgcccacc tgctcgatca ggcgcggcgc acccgccata
     7801 ttatccggca aaacctgata tgggcgggcg cgtacaacat catcgccgta ccgcttgccg
     7861 ttttgggcta tgtccaaccg tggatagccg cactgggtat gagtttcagt tcgctggcgg
     7921 ttttgggcaa tgccttgcgc cttcacaaac gggggaaaat gccgtctgaa aaaatactgt
     7981 ccgaacaatg acgggcggcg ttgctttaga cgtatagtcg attaaaacaa aaatgagacg
     8041 atgaaggatt acaaacttaa agtatgcatt gttaccgctc aaacacgttg gcgtttaaaa
     8101 tttgagatcg aattgctagg tatttg
//
LOCUS       DAXFZR010000087         8015 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000087 DAXFZR010000000
VERSION     DAXFZR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.088"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            70..3493
                     /locus_tag="ACFJAS_001834"
                     /pseudo
     CDS             70..3493
                     /locus_tag="ACFJAS_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            3708..4691
                     /locus_tag="ACFJAS_001835"
     CDS             3708..4691
                     /locus_tag="ACFJAS_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HFC3983006.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            4962..6452
                     /locus_tag="ACFJAS_001836"
     CDS             4962..6452
                     /locus_tag="ACFJAS_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC3983007.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRRLLDEGSRQTQQYRES
                     GWLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARY
                     AKLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQ
                     NKESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGIN
                     QCVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKD
                     KDSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRT
                     LSPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKA
                     YPEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRSDKQYIMM
                     AAAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(6520..7917)
                     /gene="aspA"
                     /locus_tag="ACFJAS_001837"
     CDS             complement(6520..7917)
                     /gene="aspA"
                     /locus_tag="ACFJAS_001837"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC3983008.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     INRILSPENLMNPHL"
BASE COUNT         1912 a         2318 c         2157 g         1628 t
ORIGIN      
        1 aaatttcaaa taattcttat tatgagtgta tcttaataaa attcatttta tattcttaat
       61 aaggaataca tgatgtccgg attctccccc aaacctaaga ccattatcct aagccttgca
      121 ggtgcatttg gcgcattggc ttttgcagac accccgaaca ataccgaaca gcagaaagaa
      181 ctgaatacca tagtcgtcca tggcaaacgc agtgccgacc aaaaaggcgc ggacgacgtg
      241 tattacaaaa acgtctccaa cgcctacgtc ggcaaagaat acctcgaacg ctaccgcgtc
      301 caatccgccg gcgacgtact caaaggccta aacggcgtgt acaacatgaa cacccgcacc
      361 gccggcggcg ccatcacgcc caacatacgc ggtatcaccg gcaagggccg catcccggtt
      421 accatagacg gcaccgaaca aaccatcgac gtatggatga acaactacgg cgtgggcgac
      481 cgcaactacc tcgaccccgc cctgttccgc agcatcgcgg tggaaaaaag ccccgccctc
      541 acgcgcggcg tgaaatcggg cgtcggcggc gcgatgtcca tccgcaccat cgaagccggc
      601 gacatcgtgc ccgaagggca gaaattcggc ttccagctga aaaccgagtt tgccaacaac
      661 agaacccgcc cggccaacaa cctcaaccaa tggctgggct gggaagacta ccgcaccctg
      721 ccgctcggcg ccaccgccga cggcgcgggc ggcggttctg acccgatcac cggacagcag
      781 agcccgcagg cgttggtggt agacaacttt accccgccgc agcacaaaag cggccgcgac
      841 aactggcgct tcggcggcga ccgcagttat atggccgccg ccgccttcaa aaccgaactt
      901 tcagacggcc ttgccgccta cagctaccgc aacaaaggca actacttcgc cggtcgcaag
      961 ggtgcggaag gctacctgaa taatccggta tacgacctgc aaaaatgcta cgacgaaggc
     1021 ggcagcgatt tcaactgcaa aaattccgcc accttcatcc caaatatggc gcgcatctac
     1081 caccccgggg tggaagtgct caacagcaac accgaaacca aaaccctgct gctgaaaaac
     1141 aactggcacc tgcccggcag ccatcggctc ggctggcaat atatgcgcac cgacgtgcgc
     1201 ttcggcgaga tcaacccctt ccacaccacc tatgtgatga atatggaaga gcacaacgag
     1261 accaaccgcc ccaaagaact gtcgccacag gcgcagagca tcgattccac catccgcacc
     1321 gacacctaca aactcggctg ggcctggaag ccggaaaaca gccgctgggt cgatctgcag
     1381 gccaacctgt ggcgcatcaa aaccaacagc acccgccacc agagtggcgg catggatttg
     1441 tcttccgccc gccccgatcc cttctacgac gtttggtatt ggtgtaccca acgcggccgg
     1501 ataccgcccg agcacgtcga caactacagc agctgcaacg acctgatgaa tgatttcggc
     1561 gtcaacggcc tgaccaaaga acaggtgttg gccatgaccc cgaacgacaa cggccaattc
     1621 cgcgtgattt ccggcgccga gcagaaaacc cgcgtcagcc gcaccggttt cgatatcagc
     1681 aaccgcttcc gcctcagaga ccgcctgagc atgaccttgg ccgccgatta tcagaaagag
     1741 aaactggcgg aggaagtgga aatcgtcaat tcgcaagacc tgttcaacct ggccggcatg
     1801 gtaaccggcc tgaccaagct ggccgggccc aggggcggcg agcggcgcga atggggcatc
     1861 aatttggtgt tcgactggca ggccgccgac cgcctgaaaa tctccgccgg catccgctac
     1921 cacaacttcc ggggcttcga taccgccctg gccgaagggc gtgcccgccg cgacccgcgc
     1981 tatcaggcag gcgatggaac ccagcattat gccgatggtg cctatctgcc gtatttcgaa
     2041 ctggcaggcg atcaggagcg gcgtgactgg aatgtggtgg ccgaacagct tcggcaggcg
     2101 tatcagtcag gcgatgctgc cgccattgcc gcggcagtac aggcagaaca ggcgcacggc
     2161 gcccgttacc atttgctgaa atactatgac ggctcgtatc gtaccgatgg ttatgtgtat
     2221 atcggcaacg atggccgtgc ctattttgac ggacggttcg gcggggttaa cgaggcacag
     2281 aaccaaccgc tctatcgggt gcgccccgtt tatgtgccgt ttgtcaacgg caagctggac
     2341 agcggcgcat tgccccagca ttttcatgat ttcgtcagca attatcagga aaaagtgacc
     2401 aatccgcagg ggctgcacgg gacgcattat cgatattggg caggcgtggg gaatcctatc
     2461 gactgtataa gaagtgatgc ggcctcttgc cttgctcata acctatcggg aaaagtaacc
     2521 aattttcaag tcggctatcc cgagtacggt gatgataccg gtctcggtct caaaatcatc
     2581 ggtcggcact ataccgagga acagtattgg gcgcagccca aaccgatccg tgcccatgcc
     2641 tgggcgccga ccatcgccgt cagctacgat ctgaccgaca acagccgcct gttcgtacgc
     2701 tatgcgcaga tgagccgctt ccccagcgtg tatgaggtgg gcagttttta taacgacgtg
     2761 gcctatgccg gtatgccgaa ggccccaaat ttccgcttca agccggaacg cagccgcagc
     2821 tgggaaatcg gctacagctt caattttgcc ccgtattgga gccggctgcg tgccggcgat
     2881 atgcgcctga cctactaccg caaccgtatc caaaatgtga tcgaaaccac agattatttc
     2941 cgcaccgtgc aatacgaccg caaggacacc gccgggctgg aatggcagag ccgcatcgac
     3001 accggccgct tctttgccgc gctcggtgcc acctaccgcc tgaaacagca gatgtgtgac
     3061 cgcgataccg ctttcgatta cgacccttat ggtgccaaag gcgtgccgat ctgcatcgaa
     3121 ggcggctacg gcagtacgcg cggctatcag gccttgcagc cgaaatattc aataaacctg
     3181 gatgccgggg tgcgcctgtt gggcgagaaa ctggagctcg gcctgcgcgg catctaccac
     3241 agccgggtca acaccaagca atacgaccaa ctgctgcaaa aggatttggg catcatcttc
     3301 gataccaccg gcaagcccca ccattggcgg tcatcgctga cttgggatgt ttatggccgc
     3361 taccggctgc acaaaaacct caacgtcaac ctcggcatca ccaacctgac caaccgctac
     3421 tatctcgacc cgatgtccaa cgttgccgcc cccgggccgg acggttacct tcgggctgac
     3481 tgccaagttc tagccgcagg gcggagtggg gttttcaggt agcctgcggg tatggcggga
     3541 aggctacctg aaaacggatt ggatataaca acaaacaatt gaacactccg gacgcacaaa
     3601 cccaaccggc cccgcttatc cgaggtaatg gtttttaccg ttttttccgc cggttcggcg
     3661 gtaataaaac ggtggctttt gtttaactct taattaagga aacgattatg tctatacgta
     3721 ataaaaccct gttggccgtc ttgctgtgcg gcgtttttgg tgtggctgcg gctgctccgg
     3781 ctggtggttc cagccaaagg aaaagccaag atccaaatag tgacccgacg acagttcgcc
     3841 ccggcacctc tgacaagcaa cctttcggtg ccggtaaggc cggtttggca ttcagtatca
     3901 ataacggtgg aagttggtcg aaatccatca atctggatga tggtccggtc aaatatatcc
     3961 gtgcgaaata tcaggcaccg attaacggca acgatcccta tgctaatgtt gatggtacgc
     4021 gcaatctgca atatctgcgc ttaggcgatg tgaaaccggt tatcggctgg ttctacaacc
     4081 caacgttggg tcaggtgtgg tatgaaaacc accacaacaa cactgaagtg tacagtgtgc
     4141 gccagatagc caacccggca atccccgccg cacccaagtt cggcggcctg gtaatcgcca
     4201 aagtgccggg catgggcggc agcaacggca acgtcttctt cggcgaatgg gcgccgcgtg
     4261 ccggtaaccc gcagcaaatc agcaccgacc tgaatatggc ggatggtaaa cgcaccgttt
     4321 ggtatgtggg cgataatccc accggcaaca ccaccggtgt gagaaccgcc acctacaatg
     4381 tgctgggtat caataagcac accccggggc agaacgattt ctataccggt caggtaaccg
     4441 ccaccttcgg ttctggtata accgggacga tggcaggcag aatcagccgc agttccgatc
     4501 acgtcgattt tgatcacgcc aaaatcgata acagaaatgg tacctttgcc gatcccagtc
     4561 aaggcatgac cggccgcttc tacggcaacc aggcggcagc catggccggc tataccacac
     4621 gtggtaccgg taatgtcggc gatgacgtag ctttcggcgg tagcgccacc gatattgaga
     4681 cccgcaaata acagctgcca tccgatttgg tttggcaacg gctaggctgg atttaacccc
     4741 tgaggagttt caagctgccg ttttatgccg actgccaagc catataaatc atccgtccga
     4801 tacggattgc tgctcggttt caggtagccc gtgttcaaca caggctacct gaaatttgat
     4861 atttgtaaat aaacatcgcc tatcccatac ggtttcggcc tatcggacat tttgggacag
     4921 caattaaaac ctgcgttact ttatcttttc ggaaggcaac catgaacaaa ccactcagcc
     4981 tcgccctctt ctgcctgctt ctgcccatca ccgcccacgc cgacaacccc gccgacgaac
     5041 gccgccgcct gctggacgag ggcagccgcc aaacgcagca ataccgcgaa agcggttggc
     5101 tggacacgga acaggcacgc ggggaagtag aggaaaacga cggctatatt tcgataggcg
     5161 gcgaaatcta ccaagtcggc gatacggcgg aagaactgga aagcgcaatt taccacgccc
     5221 tcaatgcgcg gcagtggcac aaggtgcggc agttcgccgc ccgctacgcc aaactgcccc
     5281 ggcacaagcc cgcgctgatt catttggccg atgcgctgca aaaacgggac gaaggggatt
     5341 tccgcgcggc ggggaacagt tttcagacgg ccttggaagc cgaacccgac aacccgcgcc
     5401 tgctgctcga agcggggcgg ttttatgccg aagacaacca aaacaaagag tctgccgccg
     5461 cgtttgagaa agttctgaaa acggacattc ccgcagaaac ccgccccatc gtggaaaact
     5521 acctgtccga actgggcaaa cgccgccgct ggcacggcca aatcagcctc ggctacggct
     5581 ataacagcaa tgtaaaccaa ggcaacggca tcaaccaatg cgtgtgggaa attgcgggca
     5641 tgtgcctgat ggagcgcacc ctgcccgcgc caacggattc gaccttctca tcctatagcg
     5701 cgaccgccga aaaaaccgtc ccgctcaaag ggcatcacgg cgtacaggtg cgcggtgtac
     5761 tgtacggcaa ccgctacacg gaaaaagaca aagattcggc ggccatgccg gattacggct
     5821 atcgcaacgg ttcgctgtac gcgggatacg cctatgccga cgcacgctcc tccttttccc
     5881 tgctgcctta tttcgaatac gacttccgca accgccacac ccactaccgc gcatggggtg
     5941 cggatgccga ctggtcgcgc acactctccc cgcactggcg cattaactca catgccggag
     6001 caaaaaagac cggatacggc ggacaaagca aaacttattt tgccgatttc aaacaatacg
     6061 aactcggcgc gggggcggaa ttttccatca cgccgaaaag cggcctgctt gtaaatttcg
     6121 atgccgcacg caaagcctat cccgaaaaat cctcttccag caaagaatat acggctcggc
     6181 tgggcgcgta taggcttttt tcgggcggta cttatttaaa cgccgtcctg ctctaccgcc
     6241 gcagcctgta tgacgcggca agtttcgtca gcgataacaa acggcggagc gacaagcaat
     6301 acatcatgat ggctgcggcg ggttttccgc aatggaatat caaaggcgta tatcccgaac
     6361 tgcgtttcag gcgaacaatc gcacacagca acgcggtgta ttaccgctac cgccaaaacg
     6421 aatggctgtt gggttttaaa tatcggtttt gaccagtcgg gtgataaaaa atgccgtctg
     6481 aaagggcgct tcctttcaga cggcatttgt ccgtaatgtt tacagatgag gattcatcag
     6541 gttctcgggg gagaggatgc ggttgatttc ttcttcgctc aacaacccgc gctccagtac
     6601 gacttcgcgc acgcctttgc cggtttgggc gcagattttg ccgaccaagt cgccgttgcg
     6661 gtggccgatg tacggattca gataagtcac cagcccgatg gagttgaaaa cgtaacgttc
     6721 gcagatttcg cggttgaccg taatgccttt gacgcatttg tcggataggt tgactgcggc
     6781 attgcccaag agggaaatgg tttcaaacat acattgggcg atgaccggct ccataacgtt
     6841 taattgcagt tgcccggatt cggcggcgaa ggtaatcgtc gtatcgttgc cgatgacttt
     6901 gaagcagact tggttgacga cttcgggaat cacgggattg actttggcag gcatgatgga
     6961 agaaccggcc tgcaattcgg gcaggttgat ttctttcaaa ccggcgcgcg gaccggaaga
     7021 gagaaggcgc aaatcgttgc agattttaga gagtttgacg gccgtgcgtt tcaatgcgcc
     7081 gtgtaccatc acatatgcac cgcagtcgga ggtcgcttcg atcaggtttt cagtcagttt
     7141 gcaaggcaag ccgctgactt cggagagttt tttgaccacc agttcggcgt agcctttggg
     7201 cgtgttcacg cccgtgccga ttgccgttgc gcccaaattg acttccaaga gcagttggcg
     7261 ggtgcggtca aggttgagga tttcctcttc caacaatact tggaaagatt ggaattcctg
     7321 gcctgccgtc atcggtacgg catcttgaag ctgggtgcga cccattttca aaacgtcttt
     7381 aaacgcttcg gctttggcgg caaaggcgtt tttcaatacg gtcagtttgt cgagcaattc
     7441 gccgatgctg taatacacgg caaggcggaa gcccgtggga taggcatcgt tggtggattg
     7501 gctggcgttg acgtgatcca tcggattgac gatgtcgtag cggcctttct catagcccaa
     7561 ggcttccaat gcaaggttgg cgatgacttc gttggtgttc atattgaccg aagttcccgc
     7621 accgccctga tacacgtcgg acgggaattg gtcgaggcag cggttgttca gcagaacttc
     7681 gtcgcaagcc ttttcaatgg cggcggcgat ttcgggtttt actgcaccca attcgccgtt
     7741 tgcctgcgcg gttgcttttt tcaccatcac catactgcgg acaaactgcg gcacgtcgga
     7801 aattttttgt gtggagattt taaagttttc aatggcgcgc agggtgtgga tgccccaata
     7861 cacttcggcg ggaatctcgc ggtcgcccaa taaatcgtgt tcgatacgga cagtcatgtt
     7921 tttacctttg taagtcggat aattaatatt gaaaaaatgc gccatcggaa agatgccgca
     7981 gcaggacgaa cactataccg gccggatgaa attgt
//
LOCUS       DAXFZR010000088         7798 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000088 DAXFZR010000000
VERSION     DAXFZR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.089"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            129..878
                     /locus_tag="ACFJAS_001838"
     CDS             129..878
                     /locus_tag="ACFJAS_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3983009.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLEVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWREDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            969..3332
                     /gene="pheT"
                     /locus_tag="ACFJAS_001839"
     CDS             969..3332
                     /gene="pheT"
                     /locus_tag="ACFJAS_001839"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC3983010.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            3406..3708
                     /locus_tag="ACFJAS_001840"
     CDS             3406..3708
                     /locus_tag="ACFJAS_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC3983011.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            3692..4000
                     /locus_tag="ACFJAS_001841"
     CDS             3692..4000
                     /locus_tag="ACFJAS_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983012.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            4176..4661
                     /locus_tag="ACFJAS_001842"
     CDS             4176..4661
                     /locus_tag="ACFJAS_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC3983013.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            4853..5077
                     /locus_tag="ACFJAS_001843"
     CDS             4853..5077
                     /locus_tag="ACFJAS_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983014.1"
                     /translation="MSVWDIWDRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMPESPDFANAVAMAVAMICIVFIYD"
     gene            5295..6596
                     /gene="bioA"
                     /locus_tag="ACFJAS_001844"
     CDS             5295..6596
                     /gene="bioA"
                     /locus_tag="ACFJAS_001844"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC3983015.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            6593..7240
                     /gene="bioD"
                     /locus_tag="ACFJAS_001845"
     CDS             6593..7240
                     /gene="bioD"
                     /locus_tag="ACFJAS_001845"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC3983016.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSHPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            7255..7731
                     /locus_tag="ACFJAS_001846"
     CDS             7255..7731
                     /locus_tag="ACFJAS_001846"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC3983017.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
BASE COUNT         1964 a         1679 c         2187 g         1968 t
ORIGIN      
        1 aaggagttaa aagattatga tgtataccta tacaaattag tactagatgg acagcaatat
       61 gttcagggaa taagaaaatc tgtattgtac gatttcctta tggaaaatca gaaagatagc
      121 tgataaaaat gaaaaaatgg actagagatg aaaccctggt tgccctttat ctttattaca
      181 tcattccttt tcaaaaggtt agtaaggaca atcctgttat tcaagaatat gctgaaatac
      241 tgggcaggac accgtccgca ctcggtatga agataggaaa tttgggtcgt ttagatccta
      301 cgctggaagt caaaaatatt tcgggattaa gtaatggcag caaaatggat gttgtcgtat
      361 ggaatgagtt ttcaggagat tgggagcaac ttaataaaga atttgaggga gtcatttccc
      421 aataccaatc aaatgatgag aatagtaata ttgaaataga atcacctgag attccaaagg
      481 gaagggaaag gtttgcaaga atatcagtac gcgtcaatca gggctttttc cgttccagtg
      541 ttttggcagc atacaataat caatgttgta ttactggatt aaaacaacct gaattgttag
      601 ttgctagtca tatcaaaccc tggagggaag ataaggataa ccgtttaaac ccaaggaatg
      661 ggctatgttt gaatgcgtta catgacaaag cttttgatag ggggttgtta ggaatagatg
      721 aaaatttcaa aattatattt tctccactac tggcaaaaac agaaggattt gatgatttat
      781 ttaaaccata tgaaaatcgg atgataagac tgcctgaaag actaaaccca agtctggaat
      841 ttttaaaatt tcacagagaa aatatttttc agagttgagt aaatatcaac attaaccaga
      901 attctaaaac agactgtctg aacgaaaatt gataaaacaa aagttaatta attgattgag
      961 aacataacat gcaattctcc tactcatggc tgaaaaccca agccaatcct gatctttctg
     1021 ccgataaact ggaacatctt ttgaccatgg ccggtttgga agtggaagaa atcgatactg
     1081 ccgcccctgc tttcagtggc gtggttgttg ccgaagtaaa atctgttgaa aaacatcctg
     1141 atgcagaccg tttgaacgtt actcaagttg atgccggtac gggcgagttg gttcagattg
     1201 tttgtggtgc gccgaatgtc aagccgggca ttaaagtgcc gtgttcgttg ccggttgccg
     1261 ttttgccggg caattttaaa attaagccga ctaaaatgcg cggcgtacca tcaaacggta
     1321 tgctgtgttc gaccaatgaa ctgggtttgc ctgatgatgg tgtagacggc ctgcacattc
     1381 tgcctgaaga tgcgcctgtc ggtaccaaca tccgcgaata cttggatttg gacgatatgc
     1441 tgtttacgtt gaaaattacg cctaaccgcg ctgattgctt gagtgttaag ggcattgcgc
     1501 gtgaggtttc tgcgttgact caatgtgcgt ttacgcctgt tgaaattcag acggccccta
     1561 tcggcagtga gaaaaaacag gctgtccgta ttgatgcacc ggctgactgc ggccgtttta
     1621 tcagccgcgt tattgaaaat gtcaacgcga aagcggctac tcctgattgg atgaaacaac
     1681 gtttggaacg cagcggtatc cgcagcattt ccgcattggt ggacattggc aactatgtga
     1741 tgctggaaat cggtcagcct atgcatgttt tcgatgctga taagctgtcg ggcagtttga
     1801 ttgtccgccg tgctcaaaat ggcgagactt tggcgtgtct gaatgagaag acggttactt
     1861 tggctgacaa tacactggtg gtcgctgatg aaaaaggtgc gttgagcttg gccggcttga
     1921 tgggtggcgc ggcgagcgcg gtttcagacg gcacgcaaaa tatcgtgctg gaagcggctt
     1981 ggtttgagcc cgaaatcatc gtcggcaaat cgcgccaata cggtttcggt tcggattcgt
     2041 cgttccgctt cgagcgcggc gtggattacc gtttgcaggc ggatgccatt gaacgcgcca
     2101 ccgaattggt tttgcagatt tgcggcggtg cggcaggcga gatggtggaa gcacaaggta
     2161 aattgcccga agccaagcag gtcggattgc gtttgggtcg tctgaaaacc gtgttgggcg
     2221 tggacattcc tgccgaacag gtggaaacca ttttgcaaca cttgggtctg cagcctgaga
     2281 aaacggcgga aggcttccgc attaccgcgc cgagcttccg tttcgacatc gaaattgagg
     2341 ctgatttgat tgaagaaatc ggacgcgttt acggctatga aaacatcccc gacgattaca
     2401 cgtcaggccg tctgaaaatg tcggaactgc ccgaaacacg ccgtccgcgt tttgccgttt
     2461 acaacgaaat ggcggcgcgc ggctaccgcg aagtgatcag ctatgccttc gttgacgagc
     2521 agtgggaact cgattttgcc gccaacgccg cccccatccg cctgcaaaac ccgctggcgg
     2581 cgcaatatgc cgtgatgcgt tctacgttga ttggcggctt agtggaaatt ttgcaaaaca
     2641 acctgaaccg caagcaaaac cgcgtgcgcg tgtttgaaat cgcctgcgtg ttcggcaaag
     2701 gttcagacgg ccggtttgtc caaaacgaac gcatcggcgg tctgtggtac ggcgcggcga
     2761 tgcccgaaca atggggtgag aaaacgcgca atgcggattt ttacgacatc aaggcggacg
     2821 tggaaaatct gttgaaaaac aaagcggtcg agttcgttaa aaccggacat cccgccctgc
     2881 atcccggacg tgccgccaat atcgtttcag acggcaaagt catcggcttt gtcggcgaac
     2941 tgcatccgaa atggctgcaa aaatacgacc tgccgcaagc gccgctggta tttgaaatcg
     3001 atatggcggc tgtgttggaa tgcgggaaaa cgcgctatcg ggccgtatcg aaattccagc
     3061 cggtgcgccg cgatttggcg tttgtgatgc cggaagttat gaatcatgat gatttactgc
     3121 ttgtcttgaa aggcgcagca aacaagttgg tacaggaaat cagcgtgttt gacgtgtatc
     3181 gcggcacggg tctgcccgaa gggatgaaga gcgtggcggt taaagtgatt ttgcaggata
     3241 tggaaaacac gctgacggat gaggcggtcg agccgcttat tggaaaactg attggcgtgg
     3301 caacggaggc aggggcgcgg cttcgcagtt aaaaaataaa tattcgcttg aattttaaat
     3361 aaaaattggt aataatccac aactgttaca acagaaggta atcatatgac actaactaaa
     3421 gcagaactgg ccgatatttt ggtggataaa gtcagcaacg tcaccaaaaa cgatgccaaa
     3481 gaaattgtcg aactcttttt tgaagaaatc cgcagcactt tggcaagcgg cgaagaaatc
     3541 aaaatttccg gtttcggaaa tttccagttg cgcgacaagc cgcaacgccc gggccgtaat
     3601 ccgaaaaccg gcgaggaagt gccgattacc gcccgccgcg tggtaacttt ccatgccagc
     3661 cagaaactca aaggcatggt ggaacactac tatgacaaac aacgttgatt tgacgattcc
     3721 tgcaaaacgc tatttcacgc tggacgagtt gtgcggactg ttgcaaatca gcccctatgg
     3781 ttttgcgcaa tggcagcatg atcacggtgt ggttgtcggt tacggcggag aacgctacac
     3841 ccgtttggat gtggtgaaac tgttgaaact gaagagcacg tttgcaccgt atgcagaagg
     3901 tgcggaatcg ggttcggacg gcaaccgtcc ggttacgctt caggaaatcg gagacggtct
     3961 gaaagacctg ttggaggatt tggataagga gttgtgctga tttgagaccg gttgcaggta
     4021 tgcagccggt tttgttttac acgctaaaaa taattattgc gtgatttcac attgtttcgg
     4081 cttgaagcac atggttttgt aatcatttac aggcagctcg cttggaatcc tgttcgggcg
     4141 gtttgctgtt tacttaaata taaggatgac ggtcaatgag atttttcggt atcggttttt
     4201 tggtgctgct gtttttggaa attatgtcga ttgtgtgggt tgccgattgg ctgggcggcg
     4261 gttggacgct gtttctaatg gcggcaacct ttgccgccgg tgtgctgatg ctcaggcata
     4321 cggggctgtc cggtctttta ttggctggcg cggcggtaaa aagtagtggg aaggtatctg
     4381 tttatcagat gttgtggcct atccgttata cggtggcggc ggtgtgtctg atgagtccgg
     4441 gattcgtatc ctcggtgttg gcggtattgc tgctgctgcc gtttaaggga ggggcagtgt
     4501 tgcaggcagg aggtgcggaa aattttttca acatgaacca atcgggcaga aaagagggat
     4561 ttttccacga tgacgatatt atcgagggag aatatacggt tgaagagcct tacggcggca
     4621 atcgttcccg aaacgccatc gaacacaaaa aagacgaata aatatgaatg gaatgccgtc
     4681 tgaaggttca gacggcattt ttccggtttg aaaatatagt ggattaacaa aaaccagtac
     4741 ggcaaggcga ggcaacgccg tactggtttt tgttaatcca ctataaattt aatccactat
     4801 aaaatagggc tgtaaccttc aatcggaatt tgttgcctgc gggatatacg gtatgagtgt
     4861 ttgggatata tgggacagga tggtggaaat ctatcataag tataagaagc cgtgcctggt
     4921 tttagcagtg gattttgtga tgggtatggt attcatagag ccgaatgagg agccgtgcat
     4981 cggcagatgc tatgcgccta tgccggagtc ccctgatttt gccaacgctg ttgcgatggc
     5041 tgttgctatg atctgtatcg tatttatcta tgattgattg aagcgttggg cggaggctgt
     5101 gtgaaacggt attgggcgtt gggtcgtctg attccaatca ggcttgagga atgcgaaacg
     5161 gtgtgcgctt atgttgcgga cgatttgttt cgcggttttt cgcccgaaac ggatggaaag
     5221 gtgtgggaag cggtctgtcg gagtagaata cgctttttgc gtttgaatgc agtaataaga
     5281 aaagagagaa acttatgccg tctgaacatc aacacacatc atcattactt aatttcgacc
     5341 gtacccatct gctccatccc tatacttcca tgaccgatcc gcttcccgtt tatcctgtca
     5401 aacgtgcaga aggggtgttt atcgaattgg cggacggcac gcggctgatt gacgggatgt
     5461 cgtcttggtg gtgtgcgata cacggttaca atcatcccgt tttgaatcag gcggttgaga
     5521 atcagatgaa acaaatggcg cacgtgatgt tcggcggcct gacgcacgag ccggcggtgg
     5581 agctgggcaa gttgttggtc gggattttgc cgcaggggct ggaccgtatt ttttatgcgg
     5641 attcgggttc ggtttcggta gaagtcgcgt tgaaaatggc ggtgcaatac cagcaggcgc
     5701 ggggtctgac ggcgaagcag aatattgcga cggtacggcg cgggtatcac ggcgatactt
     5761 ggaacgcgat gtccgtttgt gatccggaaa cggggatgca ccatattttc ggcagcgcgt
     5821 tgccgcagcg ttattttgtc gataatccga aaaaccgttt cgacgatgaa tgggacgggg
     5881 cggatttgca gcctgtccgc gccttgtttg aagcgcatca tgtggatatt gccgccttta
     5941 ttttagaacc ggtcgtgcaa ggcgcgggcg gcatgtattt ttatcatccg cagtatcttc
     6001 gcggcttgcg cgatttgtgc gacgaatttg atatcgtgtt gatttttgac gaaatcgcca
     6061 ccggattcgg gcgcacgggc aagatgtttg cctgcgaaca cgcggaggtc gtgccggata
     6121 ttatgtgtat cggcaagggt ttgagcggcg gctatatgac gctggcggcg gcaatcactt
     6181 cgcaaaaagt taccgaaacg atttcacgcg gcgaagcggg cgtgtttatg cacggcccga
     6241 catttatggc gaacccgctg gcgtgtgccg ttgcctgtgc ttcggtcaag ctgcttttat
     6301 cccaagactg gcaggcaaat atccgccgta tcgaaagcat cttaaaaggc cgtctgaaag
     6361 ccgcgtggga cattcgcggc gtgaaagacg tgcgcgtttt gggtgctatc ggcgtgatag
     6421 agttggaaaa aggcgtggat atggcgcgtt ttcaagcgga ttgtgtggcg cagggcattt
     6481 gggtacgccc gttcggcagg ctggtgtatc tgatgccgcc ctatatcatt tcagacggca
     6541 ttttgaccaa acttgccgat aaaaccgtgc aaatcttgaa ggaacacagc aaatgaaagg
     6601 cgtttacttc gtcagcggta tagacacgga catcggcaaa accgtcgcca ccggtatgtt
     6661 ggcaaaacaa ttgttgcagc agggcaaaag cgtgattacg caaaagcccg tgcaaaccgg
     6721 ttgccaagac atcgccgaag acatcgccgt ccaccgtaaa atcatgggca tacccatgca
     6781 ggaagctgat gaacagcgtc tgactatgcc tgaaatcttc agccatcccg cctcgcccca
     6841 ccttgccgcc cgactggacg gcaggggttt ggacttggac aaaatccgca ccgccacaca
     6901 agaattggcg gcgcagtacg aagtcgtttt ggtcgaaggc gcgggcgggt tgatggtacc
     6961 actgacggaa aagctgttaa ccattgatca tattcagcaa caggcttatc ccgtcatcct
     7021 tgttaccagc ggacggctgg gaagcattaa tcacaccctg ctcagtttcg tcgtgctcaa
     7081 acaatacggc attcgcctgc acagcctcgt gtttaaccac atccacgaca gccgcgacgc
     7141 acacgtcgcc caagacagcc tgaactatct gcaatgccgt ctgaaagccg attttcccga
     7201 agcggaatgg atggagttgg caaaaacagg cgcggtataa agattgggaa agatatggaa
     7261 cacctatttg ggaaatggct gcccgacttg cccgcccccg tttcagacgg catcgacctg
     7321 ccgatgagcc ggttattgaa agcccggtcg ctgaccgccg cattgtgcgc cttgccgcat
     7381 acattttcgg tggaactgct gaaactgggc gaagcggaaa cggaatacgg acggcggagg
     7441 gtgcgcgatg tgcttctgaa actggacggc actgctgttg tgcaggcacg aagcgcgtgc
     7501 agcgtcggtt cggcgttttg gcaaaacatt ttggactgcg gcacacgccc tttgggcgag
     7561 cgtctgtttc aagccgattt ggaaggggcg cgttcggcgt ttgagtttgc cgtttccagt
     7621 gaaggatgcg gacggtactt tgccgcgcgg cgttctcggt tttcccatca aggcgaggaa
     7681 atgctgctga ccgagtattt tctgcccgaa ctgaaacgct ttatcggata aaataccgtt
     7741 ttttcaagct gtgcggcaac ctgcatctaa accacatttt aatataagag actccaat
//
LOCUS       DAXFZR010000089         7757 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000089 DAXFZR010000000
VERSION     DAXFZR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.090"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(39..764)
                     /gene="aat"
                     /locus_tag="ACFJAS_001847"
     CDS             complement(39..764)
                     /gene="aat"
                     /locus_tag="ACFJAS_001847"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC3983018.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(834..1268)
                     /gene="fur"
                     /locus_tag="ACFJAS_001848"
     CDS             complement(834..1268)
                     /gene="fur"
                     /locus_tag="ACFJAS_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC3983019.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFEKHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1495..1872
                     /locus_tag="ACFJAS_001849"
     CDS             1495..1872
                     /locus_tag="ACFJAS_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC3983020.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            1882..2691
                     /gene="dapB"
                     /locus_tag="ACFJAS_001850"
     CDS             1882..2691
                     /gene="dapB"
                     /locus_tag="ACFJAS_001850"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC3983021.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2805..3074)
                     /locus_tag="ACFJAS_001851"
                     /pseudo
     CDS             complement(2805..3074)
                     /locus_tag="ACFJAS_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3165..3323)
                     /locus_tag="ACFJAS_001852"
     CDS             complement(3165..3323)
                     /locus_tag="ACFJAS_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983022.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            complement(3331..4503)
                     /gene="lpxB"
                     /locus_tag="ACFJAS_001853"
     CDS             complement(3331..4503)
                     /gene="lpxB"
                     /locus_tag="ACFJAS_001853"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC3983023.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4654..5646)
                     /locus_tag="ACFJAS_001854"
     CDS             complement(4654..5646)
                     /locus_tag="ACFJAS_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC3983024.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVGFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGDPLVLKAEPPPDLAQFAVMLENGTKM"
     gene            6139..>7757
                     /locus_tag="ACFJAS_001855"
     CDS             6139..>7757
                     /locus_tag="ACFJAS_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HFC3983025.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAA"
BASE COUNT         1870 a         2497 c         1831 g         1559 t
ORIGIN      
        1 tccactatat ttgaacctga ccgaagcata agccgccatc aaagcccctt gcacgccact
       61 tcgcgccgcc cgatttcctc tttcaacggc acggcgttca acatccgcag acgttcggca
      121 aaatccgcaa acggcagcag ctccgaaccg aaacggcgca tatgttccgt gtcctgctgg
      181 cagtctatca gttccacgcc caaatccgcc aaaaacggca cggcgcaggc aaacgcgatt
      241 ttcgacgcat ccggttgcaa cgcaaacatc gattcgccat aaaacaccct gccgatctga
      301 acgccgtaaa agccgcccgc caacctcgtt ttaccgtttt catcgggata atggcactcg
      361 aaagaatgcg cgtgtcccat ttcgtgcagc ttcaaatatg ccgtctgaaa ctcgggcgca
      421 atccaagtcc catcctgatt cgggcgcgcc gctgccgcac aatgcgcgac aacttccgca
      481 aaacagccgt tgaccgcaac ccgatagctg ccgttacgca gcgttttcgc cagcgagcgc
      541 ggaacgtgca gacgttcggg aacaattacc gcacgcggcc cgaccgcata ccaaaaaaac
      601 caaccgtccc gcgaaaacca aggaaacacg ccgttccgat acgcctcgag cagccgcccc
      661 gcgtccaaat ccctgctcac gccgaccagc ccgtcgcacc gggccaaagc ataggcagga
      721 tcgggaaagg cataattgtc aggggcaagc agcggaatac gcatggcagt tctccaaaaa
      781 cacagacggc atccaaaatc atgaatgccg tctgaacaac aaaccgtccg gatttaacgt
      841 ttgcccttgg cctgacagtc gctgcacacg ccgtacatat aaagcgcgtg atcgacgatg
      901 cggtagccgt tttcctcggc gattttgtct tgcagggctt cgatttcggg attgtggaat
      961 tccgttacct cgccgcactt cacgcagacg atatggtcgt ggtggtcgcc cttgtccaac
     1021 tcataaaccg ccttgcccgt ttcaaaatgg tggcgttgca gaatgcccgc ctgctcgaac
     1081 tgcgtcagca cgcggtaaat cgtcgccaca ccgatttcca cgccctcttc cagcagaatg
     1141 cggtacacat cttccgcact caaatgctct tccgcgtgct tctcgaacaa atccaaaatc
     1201 ttcaaacgcg ggccggtaac cttcagaccg ctgtctttca gttgcgcaat gttgctgaat
     1261 ttttccataa tattcaatat ccctgtaaaa taatagacgc tataatacgc aatttcagcc
     1321 tgcttgccca ctatcacacc atagcagttc gcaatagcaa aaccgcaacg gcggacgggc
     1381 agtcggacac agacagggta aaataccgct taagcgtatg attatcgttc gcttttataa
     1441 aatattcaag cagtgctaca ctagacatcc cgatttgcac agaaaggttc tcccgtgaac
     1501 aaaaccctca tcctcgccct ttccgccctg ttcagcctga ccgcgtgcag cgtcgaacgc
     1561 gtctcgctgt ttccctccta caaactcaaa atcatccaag gcaacgaact cgaaccgcgc
     1621 gccgttgccg ccctgcgccc cggcatgacc aaagaccaag tcctgctcct gctcggcagc
     1681 cccatactgc gcgacgcttt ccataccgac cgctgggact ataccttcaa cacctcccgc
     1741 aacggcatca tcaaagaacg cagcaacctg accgtctatt ttgaaaacgg cgtactcgtc
     1801 cgcaccgaag gcgacgccct ccaaaatgcc gccgaagccc tccgcgcgaa acaaaacgca
     1861 gacaaacaat aaggaacaca catgacaccg ctcaaaatcg ccatcgccgg cgcaaacggc
     1921 cgcatgggac gcgtcctcgt cgaagccgtc aacaaccatc ccgacaccgt cctttccggc
     1981 gcgctcgaac actcaggctc agaagccctc gggctggacg cgggctacgc cgtcggactc
     2041 aaaaccggca tcgccatttc agacgacgtt gacgccgttc tcgcccaaag cgacgtactc
     2101 atcgacttca cccgccccga gccgaccctc aagcacctgc aaaaatgcgt tgaaaaacaa
     2161 gtcaacatca tcatcggcac aacaggcttc gacgatgcgg gcaaagccgc catccgcgcc
     2221 gccgccgaaa aaacaggcat cgttttcgcc gccaacttca gcgtcggcgt caacctcacc
     2281 ttccacatcc tcgacaccgt cgcccgcgtc ctcaacgaag gctacgacat cgaaatcatc
     2341 gaaggccacc accgccacaa agtcgatgcc cccagcggca cagcattacg aatgggcgaa
     2401 gtcatcgccg gcgcgctcgg ccgcgacctc aaacaatgcg ccgtttacgg ccgcgaaggc
     2461 cacactggtc cgcgcgatcc ttccaccatc ggctttgcca ccgtccgcgc aggcgacata
     2521 gtcggcgacc acaccgccct cttcgccacc gacggcgaac gcgtggaaat cacccacaaa
     2581 gccggcagcc gcatgacctt tgccgccggt gccgtccgcg ccgcagtttg ggtaaacggc
     2641 aaaacgggtt tgtacgatat gcaggacgta ctcgggctga acaaccgtta acccccatac
     2701 aaaatgccgt ctgaaaagat attgttcaga cggcattttg ccgacaggct tcgtatcggc
     2761 atatcaataa agttgcttgt gcagcatgac aagctacggc tcgtttaatt gaaactctcc
     2821 tgatctaaaa attccaactc tatttcccgg caaactatat ctatgataaa actggtcgga
     2881 agattatacc tacgccctat taatgccagt gcacctccat aatccaagat ttcatcatcc
     2941 tgctttaatt ctccaagctc caaggtatta tcgatatctt tatcttggtt ctccgtagcg
     3001 tatccataga taaatttcag atagctgaac tgcttgaatt taatttttac ccagtctgtg
     3061 taatttggtg tagggcacaa ttgcctaaaa tagaaattta cgaagaacaa atgaaacaag
     3121 gtacgtaagc gtattagtat aaactataat catatataat aaaattaatc agttaacgaa
     3181 ccattatatt ttaaccttaa tcccattcgg gccagttctg tttcatttgg aattcctctt
     3241 aaatacttat tgtttgatgt gccattaatc acaatttctc cccctttttt tgtaatacgt
     3301 gctgcttcgg gaaaataatc cattaaccgc tcaacatccc gcctcttcca aaaccgcgcg
     3361 cgcggccaaa tccgccgtat cttttttcaa cagcaggtgc aacgccccga aatcctgttg
     3421 cagcgcggca accttatcgg ggtgttcgta ccagtccgcc aatgccgccg ccagtttttc
     3481 cggttttgct tcagattgca ataattccgg cacggcctcc ttacccaaca ggatattcgg
     3541 caggccgaca tgcggcactt tgattttgcg tttcacataa gcataggtca gcggcgaaat
     3601 cttgtagctg atgaccatcg gacgcttgca caacgccacc tccaaagtcg ccgtaccgct
     3661 cgttaccagc accgcatccg ccgccctgca caccgtttca gactgtctgt cggttaccgt
     3721 cagcgccaat ccggcaaact ccggccgctg caaaacttcc gccaaacgcc gcttcgtcgc
     3781 ctccgttgcg gcaggcagca ggaagcgggc ggcgggatag cgttccaaca acaataatgc
     3841 cgtctgaaaa aacaccggcg ccatatagtc gatttcgctg acgcggctgc cgggcagcag
     3901 ggcgaatacg gggatgccga catccgcgcc caaagttttc cgcgccgttt cacggtcgtc
     3961 ttccaagggc ataagctgtg ccatcggatg accgacaaac tccgcacgtc cgcccgcatc
     4021 gagataaagc tgcggctcca tcgggaacag gcacagcacg cggttgacct gatgcacgat
     4081 tttgcccacg cgttcgcgcc gccacgccca aaccgacgga ctgacataat gcagggtcgg
     4141 aatgcccgcc cgtttcagct tttccgccac acccagatta aaatcgggcg catcgatacc
     4201 gacaaagaca tcaggtttca acgacagcaa atcccgtacc agctccctgc gtatccgtaa
     4261 aatttccggc agccgcctga ccacttcgac aaagccgcgc accgccagcc gctcctgatc
     4321 ataaaggctc tcgaaacctt ccgccttcat cagttccccg ccgatgccgg ttaaccgtgc
     4381 ctgcgggcaa cgctttcgga tggcgcgtat caggtgcgcc cccaacaggt cgcccgacgc
     4441 ttcgccgaca ctgacggcaa tcaaagggct ttttttatca accatattcg tctgtcccca
     4501 catatagtgg attaacaaaa accggtacgg cgttgcctcg ccttgccgta ctatctgtac
     4561 tgtctgcggc ttcgccgcct tgtcctgatt tttgttaatc cactatactt tcacgccccg
     4621 atgccgtctg aagccttcag acggcatcgg ggttcacatt ttcgtcccgt tttccaacat
     4681 caccgcaaac tgcgccaaat ccggcggcgg ctccgccttc aacaccagcg gatcacccgt
     4741 gagcggatgg ttcaggtgca gctcggacgc gtgcaaaaac atgcgcttca aacccaattt
     4801 ctgcaaacgc cggttcgcct gataatcgcc gtagcgttcg tcgcccgcaa tcggacagcc
     4861 ttgagattgc agatgcacgc ggatttggtg cgtacggccc gttttcagcg ttgcccgcac
     4921 caaagtcagg tgcgacagcc cgacaccgtg caaaatgccg tctgagaaac ggcttaacac
     4981 acggaacacc gtatgcgccg actgcccgtc ctcgctgacg cgtaccatct tttcgccttg
     5041 tgcgccggta tatttgaaca ggggcagttt gacatggaaa ttgtcgtccg gcagtttgcc
     5101 cacccccagc gcaaggtaga tttttttggg gtggtcgtta cggatagctt cgtgcagttt
     5161 gacgagcgcg ctgcgtttct tcgccaccat caacaagccg ctcgtatcct tgtccaaacg
     5221 gtggaccaac tccaaatact tcgcctccgg acgggcgcgg cgcaactgtt cgataacgcc
     5281 gaaacccacg ccgctgccac cgtgtaccgc cacaccggaa ggcttattga ccaccagtag
     5341 cgcgtcgtcc tcatacacca cctcaaacgc acgcgccggt acggcggcac gcctttcaga
     5401 cggcatttcc ttctccgcca cgcgcacagg cggaatccgc accgtatctc cctccgcaat
     5461 acggctgtcg ggtttgcagc gtttcttgtt caaccgcacc tcgccggcgc ggataatgcg
     5521 gtggatatag cccttgggca cgcccttgag gatttttatc agatagttat caaggcgttg
     5581 acccgcctca tgttcggcaa cccctatcag gctgaccgaa cctttgctta tttcgtgcgt
     5641 tttcatctat aatccgaaca tccgtttcag cagaaagcgc gtccgccgcg ccccctgaaa
     5701 cggtttactt taaaacggaa ttttatatta aaaacgcact ccgcaaagca ttttccctgc
     5761 gcctgttccc agccggcagg cgcagaacgt agtcaagttt gaattgattt tgccgtttcg
     5821 gcgcggaagt aagacggcag cggagcccca agccccgaac gcaaaccgcc cgaaaacaca
     5881 ggcatcacga taacaagaag aagccggcgc ccatttttcc gaccgcagcg ttcggaacgc
     5941 ggcaggcaga ccgccgcatc gatctttata gccttcttta tccgaccctt ccgcacacgg
     6001 ttccccggaa cgtgcatccc tgcggatttt caactcaaaa actgattacc ggttttatcc
     6061 gaagacatcg gaaacccagc cttacggaat gccgaaccgg accggcgcgc ccgaatgccg
     6121 cccgtccacg aggtgatcat gaaaagaatg ttattcaacg caacgcaggc cgaagagctg
     6181 cgcgttgcca tcgtcgacgg acaaaacctg ctggatttgg acatcgaaac gctgggcaaa
     6241 gaacagcgca aaggcaatat ctacaaaggc atcattaccc gcatcgagcc gtcgctggaa
     6301 gcgtgtttcg tcgattacgg aaccgaccgc cacggctttt tgccgtttaa agaagtatcg
     6361 cgttcgtatt tcctcggcta cgaaggcggg cgcgcgcgca ttcaggacgt gctcaaagaa
     6421 ggcatggaag tcatcgtcca agtcgaaaaa gacgagcgcg gcaacaaagg cgcggcgctg
     6481 accaccttca tcagcctcgc cggacgctat ctggtattga tgccgaacaa tccgcgcggc
     6541 ggcggcgtat cccgccgtat cgaaggcgaa gagcgtcaag aactcaaagc cgcgatggcg
     6601 caactcgaca ttccgaacgg catgagcatc atcgcgcgta ccgccggcat cggccgcagc
     6661 gcggaagagt tggaatggga tttgaactac ctcaaacaac tctggcaggc aatcgaagaa
     6721 gcaggaaaag cgcatcacga cccctacctg ctctttatgg aaagctcgtt gctcatccgc
     6781 gccatccgcg actacttccg tcccgacatc ggcgaaattt tggtggacaa tcaagaagtt
     6841 tacgaccaag ttgccgagtt catgagctac gtcatgccgg gcaacgcagg tcgtctgaaa
     6901 ctctacgaag accacacgcc gctgttttcc cgcttccaaa tcgaacacca gatcgaaagc
     6961 gcgttctcgc gcagcgtcag cctgccctcc ggcggcgcga tcgtcatcga ccacaccgaa
     7021 gccctcgtct ccatcgacgt gaactccgca cgcgccacac gcggcgcgga catcgaagac
     7081 accgccttca aaaccaatat ggaagctgcc gaagaagtcg cccgacaaat gcgcctgcgc
     7141 gacttgggcg gcttggtcgt catcgacttc atcgacatgg aaaaccccaa acaccagcgc
     7201 gatgtggaaa acgtcctgcg cgacgcactc aaaaaagacc gcgcccgcgt acagatgggc
     7261 aaactctccc gtttcggact tttggaattg agccgccaac gcctgaaacc cgctttgggc
     7321 gaaagcagcc acgccgcctg tccgcgctgc gccggcaccg gcgtgatccg cggcatcgaa
     7381 tccaccgccc tgcacgtttt gcgcatggtt caggaagaag cgatgaagga caacaccggc
     7441 gaagtgcgcg cccaagtgcc cgtcgatgtc gccaccttcc tgctgaacga aaaacgcgcc
     7501 gagctgtttg caatggaaga gcgtttggat gtgaacgtcg tcctgattcc gaacatccac
     7561 ctcgaaaatc cgcactacga aatcaaccgc atccgcaccg acgacgtaga agaagacggc
     7621 gaaccgagct acaagcgcgt tgccgagcct gaagaagacg aatccgccaa acctttcggc
     7681 ggcgaaaaag ccaaagccgc ccgccccgaa cccgccgtca aaggcgtgcg ccacaccagc
     7741 cccgccccga ctgccgc
//
LOCUS       DAXFZR010000090         7586 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000090 DAXFZR010000000
VERSION     DAXFZR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.091"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(12..428)
                     /gene="dksA"
                     /locus_tag="ACFJAS_001856"
     CDS             complement(12..428)
                     /gene="dksA"
                     /locus_tag="ACFJAS_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC3983026.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
     gene            complement(576..1385)
                     /gene="proC"
                     /locus_tag="ACFJAS_001857"
     CDS             complement(576..1385)
                     /gene="proC"
                     /locus_tag="ACFJAS_001857"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC3983027.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLEKELGVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            complement(1416..1823)
                     /locus_tag="ACFJAS_001858"
     CDS             complement(1416..1823)
                     /locus_tag="ACFJAS_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC3983028.1"
                     /translation="MEIRVIKYTATAALFAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1823..2509)
                     /locus_tag="ACFJAS_001859"
     CDS             complement(1823..2509)
                     /locus_tag="ACFJAS_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC3983029.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVGLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2629..3672
                     /gene="pilT"
                     /locus_tag="ACFJAS_001860"
     CDS             2629..3672
                     /gene="pilT"
                     /locus_tag="ACFJAS_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC3983030.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            3836..5062
                     /locus_tag="ACFJAS_001861"
     CDS             3836..5062
                     /locus_tag="ACFJAS_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC3983031.1"
                     /translation="MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5179..7329
                     /gene="yccS"
                     /locus_tag="ACFJAS_001862"
     CDS             5179..7329
                     /gene="yccS"
                     /locus_tag="ACFJAS_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC3983032.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIRRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1824 a         2552 c         1683 g         1527 t
ORIGIN      
        1 gtttgcctcc atcagtcggc aaactgtttc ttcatcctct ctcggcgttc ttgggcctca
       61 acggataaag tggctgtcgg gcgtgccagc agccgcttca gaccgatagg ttctcccgta
      121 tcggcacaga atccatagtc cccttcatca atattgcgga tggtcgcctg tattttactg
      181 agaagttttc gttcccgatc ccgggtacgg agttccaatg cgtactcttc ttcctgtgtg
      241 gcacggtcgg caggatcggg ggctgattcg tgttcttgga gatgccctgt tgtaacggag
      301 gcgttttcga tgagttcgtc ttgcattttt accagcaatt cgcggaaaaa agccaaatgg
      361 tcgtcattca tataatcgtc ttccggccca atccaattca aaatatcttg ttctgtcagc
      421 tttaccataa tgtatgccct ctcggttcac cgcaaaaaaa caaagcacta caaagtggct
      481 ttattccttt gatttttata aggcttattg cctaaggcgt ttttgaaaac gcgcatcata
      541 acacgttttg gattggtttt tttattttct ttacattatt gatattgccg ttccatttcc
      601 tgcgaacggc acacacaggc acaaacgccc tcacttatgg cttcggcgac acgatgccgt
      661 ctgaaggctt ccacggcttc gtgggttgtc ccgccttttg acgttacatt tttttgaagc
      721 ttctcgaaat cttcacccgt ctgctcggca agggcaaccg ctcctttaaa cgttgccaga
      781 ctgagcgcgc gtgcttctgc catatcaaac ccttgtcgga tggcggcatt ttgcaatgcg
      841 tccagcagat aaaacacata agccggcccg ctgccgctga tgccggtaat gctgtgcatt
      901 tgcgcttcat cttccaacca aacggtcaaa ccgactgatt tcatgattcg atcggcaatc
      961 ctgcggtctg tttccgatac ttccgcttcg gcatacatac cggatacgcc cagcccgatt
     1021 tttccaggtg tattaggcat aacccggaca atgcggcggc ttcccccgag gtaacggctg
     1081 agcgtaccga ccgacaatcc ggctgcgaca gaaagcacca atgcgccgtt ggtgcggata
     1141 tttttgcacg cggcttccat atcctgcggt ttgacggcaa ggattaaaac atcgtcggaa
     1201 tgaagctccg gcagggttgc cgaagtttcg acccccaact ctttttccaa acgttcgcgc
     1261 ttttccgcac cacggttggc tatatggatg cggtatcccc cttgtttgac caatccgccc
     1321 gcaacggcag ccgccatatt tccgccgccg agaaaataaa cattcattat ttctacccta
     1381 ttcaaaatta ccttactaaa ttcattccca attttttacc atcggacgcg ctcggacagg
     1441 ccgttgcgcc gcaacccctg cttttccaaa cagtcgaaac cttcctgttt gaacctctca
     1501 accaaaggtt tgccgtcacg cgtacgccaa tccaacccgc aggcttcaaa cttccctatt
     1561 ttcctataaa agtaagattt ttgcgcggat tcatattcgt ttgccctgac ggaacgattg
     1621 ccgtctgaaa gcggtatctc gtagtcctct aaagacagcg gactctcgcc gccaatatcc
     1681 caaaaatcga tggcggcagg ttttctcggc ttacaccagc cggacaagga cgaacactca
     1741 taccaccccg ccagccggca gcctgcaacc gtaaatgcaa acaacgcagc cgttgccgta
     1801 tattttatta cccgaatttc catcagcccc ttttcccgaa aatcgcgctg ccgatacgga
     1861 cgtgtgtcgc accgcactca atggcggcag gcatatcgtc cgacatcccc atagacagca
     1921 cgtccgcctt aacgccagcc gcattgaggt cggcaagcag cttttgcatg gtctgaaact
     1981 gagttttcaa ctccgtttca ctgctgttgg ctttggcaac gcacatcagt ccgcgcacga
     2041 caaggttggg cagcttcgcc acctccacgg caagcgcgac cgcttcttcg ggcgcgacac
     2101 cgtgttttgc cgcctcgccc gcaatgttca cctcgacaca cacctgcaag ggcggcatta
     2161 aggagcgacg ctgcctgctc agccggacgg cggtcttcag acggcatacg gtatgcaccc
     2221 aatgcgcgcg ttcggcgaca aatttggttt tgttggactg cacatcgccg atgacgtgcc
     2281 acacgatgtc ggtcaaatcc gccaattcct ccgttttgcc gtaccactcc tgaatatagt
     2341 tctcgccgaa atcacgctgt ccggcagcgt aaacttcgcg gatgccgtct gaagggaagg
     2401 tcttgctgac ggcaatcagg ccgacggaat gcggctccct gcccgcctgc agaaccaatt
     2461 ttccgatacg gtcggacacc tcacgataac gttcttgcaa caccgtcata gatcatcccc
     2521 taattaaaaa tgattaaaca gttgaaaccc ctccagtcag ggcggtataa tcaaggttgt
     2581 tagaatcatt ccaaccaatc gaaacattat actaaacaga gccgcattat gcagattacc
     2641 gacttactcg ccttcggcgc taaaaacaaa gcatccgacc ttcacctgag ttcgggcata
     2701 tcccctatga ttcgggttca cggcgacatg cggcgcatca accttcccga aatgagcgcg
     2761 gaagaggtcg gcaatatggt aacttcggtg atgaacgacc accagcggaa aatctaccag
     2821 caaaacttgg aagtcgactt ctcgttcgaa ctgcccaacg tcgcccgatt ccgcgtcaac
     2881 gccttcaaca ccggccgcgg ccccgccgcc gtattccgca ccattcccag caccgtctta
     2941 tcgctggaag aattgaaagc cccgagcatt ttccaaaaaa tcgcagaatc gccgcgcggc
     3001 atggtattgg ttaccggccc taccggttcg ggcaaatcga ccacgcttgc cgcgatgatc
     3061 aactacatca acgaaaccca gccggcacac atcctgacca tcgaagaccc gatcgaattc
     3121 gtccaccaaa gcaaaaaatc cctgattaac caacgcgagc tgcaccagca caccctcagc
     3181 ttcgccaacg cgctgagttc cgcattgcgc gaagaccccg acgttatcct tgtcggcgag
     3241 atgcgcgacc ccgaaaccat cggcttggca ctgaccgccg ccgaaaccgg acacttggtt
     3301 ttcggcacgc tgcacacgac cggcgcggca aaaaccgtcg accgtatcgt ggacgtattc
     3361 cccgccggag agaaagaaat ggtgcgttcc atgctgtccg aatcgctgac cgccgtcatc
     3421 tcccaaaacc tgctgaaaac gcacgacggc aacggccgtg tcgcctcgca cgaaatcctg
     3481 attgccaacc ccgccgtccg caacctcatc cgcgaaaaca aaatcacgca gattaactcc
     3541 gtcctgcaaa ccgggcaggc gagcggtatg cagacgatgg accaatcgct gcaatcgctg
     3601 gtgcgccaag ggctgatcgc accggaagcc gcacgcagac gcgcgcaaaa cagcgaaagt
     3661 atgagtttct gacacacaac cgccttccgg ccataccggc gggaaaacaa ggcgcaaaca
     3721 cgccgggcgg gacgcggcat cccgcccggc atacccttcc gaacaggaag cgtccgcctt
     3781 cctgttgaaa cctgccgccg caaactgcaa ggcttaaacc gaaaagaagc taacgatgaa
     3841 taccgataac ctgcacgaca tcttggacga aacggttcaa gtgtattctc aaaaaaaaca
     3901 aagccgatcc gaaaccccgg ccgaaatcgg cgcacacttc cacccgctgc tcgaccgcct
     3961 gtgcgaaacc gcagaagcac aaaacgcgtc cgacatcctt atcagcaaag gattcccgcc
     4021 ctcgttgaaa atcaacagcg cattaacccc gcagccgcaa aaggctctga cgggcgagga
     4081 aaccgccgcc atcgccgcat cgacgatgaa cgccgaacaa tcggaaatat tccggcgcga
     4141 cggcgaaatc aactactccg tccaatcgcg cagcggcacg cgctaccgcg ccaacgccta
     4201 ctacagccaa ggcagcgcag gtttggtttt gcggcgcatc aaccacgtca tcccgcaaat
     4261 gcgggaattg ggcttgcccg aaaaactcaa agacctcgcc gtcgcaccgc gcgggctgct
     4321 gattatcgtc gggcctaccg gttcgggcaa atccaccacg atggcgacta tgctcgaaca
     4381 ccgcaacaaa accctgcccg gccatatcgt taccatcgaa gacccgattg agtttatcta
     4441 caaaccgcgc cgctgcatct ttacccagcg cgaaatcggc gtcgacacca taaactggca
     4501 gacggcggta caaaacgcta tgcgccaatc cccagacgtg gtctgtatcg gcgaagtccg
     4561 cagcagggaa agtatggaat acgcgatgca gctcgcccaa accggccacc tgtgcatttt
     4621 tacgctccac gccaacaccg cgccgcagtc gctcgaacgc atactcaact tctaccccaa
     4681 agaacagcac aaccaaatac tgattgacat cgccctcaat ctgaccggca tcatctgcca
     4741 acgcctcgcc ctcaaaaaag acaaaacggg caggacggcg gttgtcgact tgctcatcaa
     4801 cacgcccgcc atccaagact tcatcctgaa gggcgacctg atgaacatca gtaaaatcat
     4861 ggaaaccgcc aaaaccgacg gaatgcagac gatggaccaa aaccttttcg aactgtaccg
     4921 tcacggcatc atcagttacg aagaagccct gcgccagtcc gtttccgcca acaacctgcg
     4981 attgcacatc caactgcaca aagaaggcaa aacgcccgaa ctcctttacg acagggtcaa
     5041 cggtctcaac ctcatttcct gatccgcaaa acccaatgcc gtctgaaaac cgcatccccg
     5101 ttttcagacg gcatgatttt atccgcccca ttcatgtgcc acactttatt cacttcataa
     5161 caataaaccg gtaaaaccat gaaaacccca ctcctcaagc ctctgctcat tacctcgctt
     5221 cccgttttcg ccagtgtctt taccgccgcc tccatcgtct ggcagctagg cgaacccaag
     5281 ctcgccatgc ccttcgtact cggcatcatc gccggcggcc tggtcgattt ggacaaccgc
     5341 ctgaccggac ggctgaaaaa catcatcgcc accgtcgccc tgtttaccct ctcctcgctc
     5401 acggcgcaaa gcaccctcgg cacagggctg cccttcatcc tcgccatgac cctgatgacc
     5461 ttcggcttta ccattttagg cgcggtcggg ctgaaatacc gcaccttcgc cttcggcgca
     5521 ctcgccgtcg ccacctacac cacgcttacc tacacccccg aaacctactg gctgaccaac
     5581 cccttcatga ttttatgcgg caccgtactg tacagcaccg ccatcatcct gttccaaatc
     5641 atcctgcccc accgccccgt ccaagaaagc gtcgccaacg cctacgaagc actcggcggc
     5701 tacctcgaag ccaaagccga cttcttcgac cccgatgagg cagcctggat aggcaaccgc
     5761 cacatcgacc tcgccatgag caacaccggc gtcatcaccg ccttcaacca atgccgttcc
     5821 gccctgtttt accgtttgcg cggcaaacac cgccacccgc gcaccgccaa aatgctgcgc
     5881 tactacttcg ccgcccaaga catccacgaa cgcatcagct ccgcccacgt cgactaccaa
     5941 gagatgtccg aaaaattcaa aaacaccgac atcatcttcc gcatccgccg cctgctcgaa
     6001 atgcagggac aagcctgccg caacaccgca caagccctgc gcgcaagcaa agactacgtt
     6061 tacagcaaac gcctcggacg cgccatcgaa ggctgccgcc aatcgctgcg cctcctttca
     6121 gacggcaacg acagtcccga catccgccac ctgagccgcc ttctcgacaa cctcggcagc
     6181 gtcgaccagc agttccgcca actccgacac agcgactccc ccgccgaaaa cgaccgcatg
     6241 ggcgacaccc gcatcgccgc cctcgaaacc ggcagcttca aaaacacctg gcaggcaatc
     6301 cgtccgcagc tgaacctcga atcaggcgta ttccgccatg ccgtccgcct gtccctcgtc
     6361 gttgccgccg cctgcaccat cgtcgaagcc ctcaacctca acctcggcta ctggatactg
     6421 ctgaccgccc ttttcgtctg ccaacccaac tacaccgcca ccaaaagccg cgtgtaccaa
     6481 cgcatcgccg gcaccgtact cggcgtaatc gtcggctcgc tcgtccccta cttcaccccc
     6541 tccgtcgaaa ccaaactctg gatcgtcatc gccggtacca ccctgttctt catgacccgc
     6601 acctacaaat acagtttttc caccttcttc atcaccattc aggcactgac cagcctctcc
     6661 ctcgcaggtt tggacgtata cgccgccatg cccgtgcgca tcatcgacac cattatcggc
     6721 gcatcccttg cctgggcggc ggtcagctac ctgtggccag actggaaata cctcacgctc
     6781 gaacgcaccg ccgcccttgc cgtatgcagc agcggcacat acctccaaaa aattgccgaa
     6841 cgcctcaaaa ccggcgaaac cggcgacgac atagaatacc gcatcacccg ccgccgcgcc
     6901 cacgaacaca ccgccgccct cagcagcacc ctttccgaca tgagcagcga acccgcaaaa
     6961 ttcgccgaca gcctgcaacc cggctttacc ctgctcaaaa ccggctacgc cctgaccggc
     7021 tacatctccg ccctcggcgc ataccgcagc gaaatgcacg aagaatgcag ccccgacttt
     7081 accgcacagt tccaccttgc cgccgaacac accgcccaca tcttccaaca cctgcccgac
     7141 atgggacccg acgactttca gacggcattg gatacactgc gcggcgaact cggcaccctc
     7201 cgcacccgca gcagcggaac acaaagccac atcctcctcc aacagctcca actcatcgcc
     7261 cggcagctcg aaccctacta ccgcgcctac cgacaaattc cgcacaggca gccccaaaac
     7321 gcagcctgaa aaaatttcgg cattttgtaa aggggggcgg attgtcaggc aggttgcaag
     7381 atagtggata aggctttgcg cagggtaaat gcgtagcaac tgaaccgtca ttccctcaaa
     7441 aacagaaaac caaaaacaga aacctaaaat cccgtcattc ccacgaaagt gggaatccgg
     7501 ttcgctcggt ttcgcttgtt ttaagtttcg ggtaacttcc acttcgtcat tcccgcgcag
     7561 gcgggaatcc agtgcgttga gtttca
//
LOCUS       DAXFZR010000091         7373 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000091 DAXFZR010000000
VERSION     DAXFZR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.092"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..423
                     /locus_tag="ACFJAS_001863"
     CDS             <1..423
                     /locus_tag="ACFJAS_001863"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC3983033.1"
                     /translation="GERWLSAMSARLARFVPDEGERRRLLVNIQYESSRAGLDTQIVL
                     GLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRYGCTILRHYR
                     NLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            535..1917
                     /locus_tag="ACFJAS_001864"
     CDS             535..1917
                     /locus_tag="ACFJAS_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC3983034.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTAISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            1921..2445
                     /gene="ssb"
                     /locus_tag="ACFJAS_001865"
     CDS             1921..2445
                     /gene="ssb"
                     /locus_tag="ACFJAS_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC3983035.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQNQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            2818..3741
                     /locus_tag="ACFJAS_001866"
     CDS             2818..3741
                     /locus_tag="ACFJAS_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC3983036.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLTLGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRPFKRR"
     gene            complement(3806..5194)
                     /gene="fumC"
                     /locus_tag="ACFJAS_001867"
     CDS             complement(3806..5194)
                     /gene="fumC"
                     /locus_tag="ACFJAS_001867"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC3983037.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(5338..7317)
                     /gene="tkt"
                     /locus_tag="ACFJAS_001868"
     CDS             complement(5338..7317)
                     /gene="tkt"
                     /locus_tag="ACFJAS_001868"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC3983038.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPQNF
                     DQYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
BASE COUNT         1432 a         1884 c         2252 g         1805 t
ORIGIN      
        1 ggcgaacgtt ggttgtccgc gatgtcggca cgtttggcaa gattcgtccc cgacgagggg
       61 gagcggcgca ggctgctggt caatatccaa tacgaaagca gccgggccgg tttggatacg
      121 cagattgtgt tggggctgat tgaagtggaa agcgcgttcc ggcagtatgc gataagcggg
      181 gtcggcgcgc gcggcctgat gcaggttatg ccgttttgga aaaactacat cggcaaaccg
      241 gcgcacaacc tgttcgatat ccgtaccaac ctgcgttacg gctgcaccat cctgcgccat
      301 taccgaaacc ttgaaaaggg cgacatcgtc cgcgcacttg cccgcttcaa cggcagcctc
      361 ggcagcaata aatatccgaa cgccgttttg ggcgcgtggc gcaaccgctg gcagtggcgt
      421 tgattttgaa cccgcgccgc aaccgaaata cggcgaatcc tgtataatcc gaaaatctgt
      481 tcactggaag ttcagacggc attgcaactg ttgatgccgt ctgaaaaatt atttatggca
      541 agagacaacc gcatccaaat gtttccgcac gaatggcgcg ccagtacgac gctttccggc
      601 gtgtacgcgc tgcgtatgct gggtatgttc ctcgtgctgc ccgttttggc aatatatgcc
      661 gcctcgctgc ccggtgcgga agacaataaa acgctggtcg ggctggcaat gggcatttac
      721 gggctgacgc aggcattgct gcaactgcct ttgggtatcg cttccgacaa gttcgggcgc
      781 aagaaaacca tttatgtcgg gctggtcgtg tttgccgccg gcagcttcct tgccgccgcc
      841 gccgatacgc tgcccatgct ggttgccgcg cgcgccatac agggcgcggg ggcggtcagt
      901 gctgccgtaa ccgcgctgct tgccgacttg acgcgcgacg gcgtgcgtac ccgcgcgatg
      961 gcgatgatcg gtttgagtat cggtctgacg ttttcggtca gcctggtggt tgcgccgatg
     1021 attgccgatg tggccggcgt tcgcggactg tttatgctga ccggcattct gaccgccatc
     1081 agcatcggcg tagtggcgtg gatgactcct gatcccgaag tttccaagct gcatgaggac
     1141 acgcaggcgc agccttcgcg cataggagaa gttttgaaaa accgtaggct gctgacgctc
     1201 gatttcggca ttttcgccct gcacgccgcg caaatggcat tgtttaccgc gctgcctttc
     1261 gcgatgaccc ggctcggttt ggaaaaaatc cagcattgga aagtatatct gccctcgacc
     1321 attacgggct tggtggtgat ggttccgctg attatcgtcg gcgaaacgcg caacaagctc
     1381 aaacaggttt ttgttttggg catcgtctgc atcgcggcgg cgcagcttgg tttgctgtcc
     1441 ggaatgcact cgatttggct gattaccgct tatctggttg tttactttat cggttttaat
     1501 gtgttggaag cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga cctgaagggt
     1561 acggcgatgg gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg cggcgcggca
     1621 ggaggcttgc tgtttcaaaa atacggcttt gccggcgtgt ttgccttttg cagcatattg
     1681 atgctgctgt ggctggtgat tgccgtttta tcgcccgcgc ccaagcccgt caaaaacctc
     1741 agttaccctg tcggcggcgt gtggcagggc aatcgggacg gtttgcaacg cgccctgttg
     1801 cagttggaag gcgtggaaga catcggtttc agtttcgacg ggcagaccgt ctatcttaaa
     1861 gtgttgcaga agggtttcga tcaggctgcc gctgaaaaaa tcattacagg agtttaaaaa
     1921 atgtcattga acaaagtcat ccttatcggc cgcctcggac gcgatcccga agtgcgctat
     1981 atgcccaacg gcgaggcagt gtgtaatttc agcgtcgcca ccagcgaaac ttggaacgac
     2041 cgcaacggcc agcgcgtaga gcgcaccgaa tggcacaaca tcacgatgta ccgcaaactg
     2101 gcggaaattg ccgggcaata cctcaaaaaa ggcgggctgg tttatctgga aggcagaatc
     2161 caaagccgca aataccaagg caaagacggc atcgaacgca ccgcttacga tattgtcgcc
     2221 aatgaaatga aaatgttggg cgggcgcaat gaaaacagcg gcggcgcgcc ttacgatgaa
     2281 ggttacggtc agaatcagga ggcttaccaa cgccccgctc agcaaagccg gcagcccgct
     2341 cccgacgcgc cgtcccatcc ccaagaagca cccgccgcgc cgcgccgcca acccgtgccg
     2401 gccgccgccc cggtcgagga cattgacgac gacatcccgt tctgaatttt acggccggac
     2461 atcctcgcgg agggaagtca taaaggacgg aaaaacctta aaccttacgg ggcaaggttt
     2521 ctcttttttg catttgggcc ggcttgttgc cgccccctgc gttgggcgta acgttgcgat
     2581 tgcgccgttc gggcgttttg aggcaagcga tgcctgccgc actcccccgc tgttttcaga
     2641 cggcatttta aaattgaggc ggtgttttca aatagtgcga agcaatagtc gggcttcagc
     2701 cagtttgttt gaaatcaaag ggttggaatc aaacgccaaa cgcttgaacc atcattgccc
     2761 gatgcggcaa accggcatcg tccggcaata cggttgttta aaaataggga aatcaggatg
     2821 gaaaaccaaa ggccgctcct aggcttcgcg ttggcacttt tggcggcgat gacgtggggg
     2881 atgctgccga ttgccgtgcg gcaggtattg aagtttgtcg atgcgccgac gctggtgtgg
     2941 gtgcgtttta cagtggcggc ggcggtattg tttgttttgc tgacattggg cgggcggctg
     3001 ccgaagcggc gggatttttc ttggcattca ttcaggctgc tgctgctcgg cgtgacgggc
     3061 atttcggcaa actttgtgct gattgcccaa gggctgcatt atatttcgcc gaccacgacg
     3121 caggttttgt ggcagatttc gccgtttacg atgattgttg tcggcgtgtt ggtgtttaaa
     3181 gaccggatga ctgccgcgca gaaaatcggt ttggttttgc tgcttgtcgg tttgcttatg
     3241 ttttttaacg acaaattcgg cgagttgtcg ggtttgggcg cgtatgcgaa gggcgtgttg
     3301 ctgtgtgcgg caggcagtat ggcctgggtg tgttatgccg tggcgcaaaa gctgctgtcg
     3361 gcgcaattcg gtccgcaaca gattctgctg ttgatttatg cggcaagtgc cgccgtgttc
     3421 ctgccgtttg ccgaaccggc acacatcgga agtttggacg gtacgttggc gtgggtttgt
     3481 tttgtgtatt gctgcttgaa tacgttaatc ggttacggct cgttcggcga ggcgttgaaa
     3541 cattgggagg cttccaaagt cagcgcggta acaaccttgc tccccgtgtt taccgtaata
     3601 ttttctttgc tcgggcatta tgtgatgcct gatacttttg ccgcgccgga tatgaacggt
     3661 ttgggttatg tcggcgcact ggtcgtggtc gggggtgcgg ttacggcggc ggtgggggac
     3721 aggccgttca aacgccgcta gttcgcaggc aacggaaaat gccgtctgaa cgaggcttca
     3781 gacggcattt tatttgaggg aaggattagc gcggatgaac catatcggca gggacgacca
     3841 gttcgtcaaa ctcttcgccc gtcagcaagc ccaacccaac ggcagtttcg cgcagcgatt
     3901 tatcgttttt gtaggcggtt ttggcgactt tggcggcgtt ttcgtagccg attttgcggt
     3961 ttaacgcagt aaccagcatc agggaatggt gcaggaaata gtcgattttt tccggtacgg
     4021 gttcgatgcc ggcggcgcag ttttcgttga agctgttgca cgcgtcgccc aagaggcgga
     4081 tggattgcaa gaggttgtag gcgataacgg gcatatagac gttcagctcg aaattgcccg
     4141 acgcacccgc cataccgatg gtaacgtcgt tgccgaacac ttggcagcac accatcgtca
     4201 tcgcttcgca ttgggtcggg ttgactttgc ccggcataat ggacgaaccc ggttcgtttt
     4261 cggggatttt gatttcgccc aagccgcagc gcgggccgct tgccagccag cggatgtcgt
     4321 tggcgatttt gttcaggctt gccgccagcg ttttcaatgc gcccgaagcg gcaacggcgg
     4381 catcgcgtcc gcccaaggct tcaaatttgt tcggcgcgct gacaaacggc aggccggaca
     4441 gttcggcgag tttggcggcg gctatttcag cgtattcggg atggctgttc aaacccgtac
     4501 cgaccgccgt tccacccaag gcgagttcgt acaaaccttt gagcgcgtcg ttcagacggc
     4561 ctaagctgtg gtcgagttgg gaaacgtagc cggaaaattc ttgtcccaaa gtcagcggcg
     4621 tcgcatcctg caagtgggta cggccgattt tgacgatggg ggcgaaggct tgggcttttt
     4681 tgtccaatgt atcgcgcaag gcttttaccg ccggaataag gtggcggttg atttcaatcg
     4741 cggcggcgac gtggatggcg gtcgggaacg cgtcgttggt cgattgcgcg tggttcacat
     4801 ggtcgttggg gtggacgggc cggtacgccg ccaaacccgt acctgcaatt tcgttggcgc
     4861 ggtttgccag cacttcgttc atgttcatat tggactgcgt accggagccg gtctgccaaa
     4921 ccactaaggg gaactgcccg tcgagcctgc cgttcagtac atcatccgcc gcctgcgtaa
     4981 tcaaatccgc ctgttcgggc ttaatcctgc cgagggaaac attggtcgcg gcggcggctt
     5041 ttttcaccaa cgccaaagcg taaatcagcg gctgcggcag ggtttcgccg ccgattttga
     5101 aattgttgcg gctgcgctgg gtctgcgcgc cccaataggc ttcggacggg acttcgacat
     5161 tgcccatcgt gtcgtgttcg gtgcgggtgt tcatacgttt ctccttttga aatgtgaata
     5221 agagtgattc gcaaatatta taatggagat tggcggaatg aggaagcatc aaggcgggag
     5281 ggcaaaaaaa tgccgtctga aaagcattca gacggcattt gatgcggcgg ctgaggttta
     5341 cagcacggat ttcaccgtat caaccacatt gtccacggta aagccgaatg ctttgaacag
     5401 cagatcggca ggggcggatt cgccgaagcg gttgatgccg acgactgcgc cgttcagtcc
     5461 gacatatttg taccagccgt tggtgtgtcc ggcttctacg gcgatgcgcg gcaggccttc
     5521 cggtaggacg gcggcttgat aggcggcgtc ttggcggtcg aagacgctgg tggacggcat
     5581 ggaaacgacg cgcacggcaa taccttgtcc tgccaatact ttttgcgctt ctacagccaa
     5641 tcctacctcg gaaccggtgg caatgatgac ggcttgggcg ttgccttggg cttcactgat
     5701 tacgtaagca ccgcgtttga tgtcgttcag ttgttgctcg ctgcgtgctt ggaatttcag
     5761 gttttgacgg ctgaaaatca ggcaggacgg gtggtcttcg gcttttgccg cttctgccca
     5821 agcgaccaga gattctgcgg tgtcgcacgg ccgccatacg tccatattcg gaatcaggcg
     5881 cagggtggcg gtttgctcga taggctggtg ggttgggccg tcctcgccca aaccgatgga
     5941 atcgtgggtg aatacgaaga cggggttgat tttcatcagt gccgccatgc gtaaggcgtt
     6001 gcgttcgtat tcgctgaaca tcaggaaagt cgcgccgaag ggttttacac cgccgtgcaa
     6061 taccaaaccg ttcataatcg cacccatacc gaactcgcgc acgccgtagt ggatgtagtt
     6121 gccgcctttg tcgcgggtaa cggagacgct gtttgaccag tcggtcaggt tggacggggt
     6181 caggtcggca gaaccgccca ccagttcggg cagctctttt gccaagattt cgatgctgtt
     6241 ttggctggct ttgcgggtgg cgacggtttc cgctttggcg cacacttctt tcaatgccgt
     6301 ctgaacgtac tggtcaaagt tttgcggcag ctttttatcc atgcggcgca caaattcagc
     6361 ggcttcggca ggatatttgg cttgatattg cgcgaacagt tcgttccatc cggcttccag
     6421 tttcgcgcct ttttctttgg cattccacgc atcgtaaatt tcttgcggga tttcaaacgc
     6481 aggataagcc caacccaaat gtttgcgcgt ggcttcgatt tcgtccgcac ccaaaggcgc
     6541 gccgtgggtt ttgtggctgc cttctttgtt ggcgctgcct ttgccgatta aggttttgca
     6601 gcagatgatg gacggcttgc cggtttcggc gcgggcggct tcgatggccg tctgaatggc
     6661 ggcggtgtcg tggccgttta cgttgggaac gacgtgccag ccgtagcttt caaagcgttg
     6721 cgggatgttt tcggtaaacc agccgtccac tttgccgtcg atggagatat tgttatcgtc
     6781 atacaaaaca atcagtttgc ccaagcccaa ggtgccggcg agtgagcagg cttcgtgcga
     6841 tacgccttcc atcaggcagc cgtcgcccat aaagacgtaa gtgtagtgat cgacgatgtt
     6901 caaaccgtct ttattaaatt cggcggcaag gattttttct gccaatgcca tgcccactgc
     6961 gttggcaata ccttgcccca acgggccggt cgtggtttcc acgccgtcgg tgtagccgta
     7021 ttcgggatgg ccgggggttt tgctgtgcag ctggcggaag tttttcagat cttcaatgct
     7081 caggttgtag ccggtcaggt gcagcaggct gtacaacagc atagacgcat gaccgttgga
     7141 gaggacgaag cggtcgcggt tgtagaattt gggattggcg ggattgtgat tgaggaattt
     7201 cgtccacaat gtttccgcca tttccgccat acccataggc gcgccggggt ggccggaatt
     7261 ggctttttga acggcatcgg ccgagaggaa gcggattacg tttgccagtt gagacatttt
     7321 gtattttcct tgctggtgtt tcggataagt ggataatcgg aaggcgttga tta
//
LOCUS       DAXFZR010000092         7173 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000092 DAXFZR010000000
VERSION     DAXFZR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.093"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            52..2217
                     /gene="fetA"
                     /locus_tag="ACFJAS_001869"
                     /gene_synonym="frpB"
     CDS             52..2217
                     /gene="fetA"
                     /locus_tag="ACFJAS_001869"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC3983039.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVGGENSRITIKR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRIATRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFKISDKEKGPNGTEVD
                     VDVAQKAKNRANEKIVYAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSSLNPSFGVIWQPREHWSFSASHNYAGRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNAGYIKNH
                     GYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQTGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKAVGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            complement(2377..2439)
                     /locus_tag="ACFJAS_001870"
                     /pseudo
     CDS             complement(2377..2439)
                     /locus_tag="ACFJAS_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2523..3488
                     /locus_tag="ACFJAS_001871"
     CDS             2523..3488
                     /locus_tag="ACFJAS_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3983040.1"
                     /translation="MLRLTTLTLCTVLALGACSPQNSDPAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVVLVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            3506..3697
                     /locus_tag="ACFJAS_001872"
     CDS             3506..3697
                     /locus_tag="ACFJAS_001872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983041.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            3716..4684
                     /locus_tag="ACFJAS_001873"
     CDS             3716..4684
                     /locus_tag="ACFJAS_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC3983042.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     KNEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLMGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4674..5648
                     /locus_tag="ACFJAS_001874"
     CDS             4674..5648
                     /locus_tag="ACFJAS_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC3983043.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFDFELVIMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5693..6319
                     /locus_tag="ACFJAS_001875"
     CDS             5693..6319
                     /locus_tag="ACFJAS_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC3983044.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            6403..7161
                     /locus_tag="ACFJAS_001876"
     CDS             6403..7161
                     /locus_tag="ACFJAS_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC3983045.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
BASE COUNT         1652 a         2162 c         1866 g         1493 t
ORIGIN      
        1 accttaacgg tttggcttaa cttcccttca tacactcaaa aggacgaaca aatgaacgcc
       61 ccgtttttcc gcctcagcct gctctcgctc acacttgccg ccggctttgc ccacgcggca
      121 gaaaataatg ccaatgtcgc attggatacc gttaccgtaa aaggcgaccg ccaaggcagc
      181 aaaatccgta ccaacatcgt tacgcttcaa caaaaagacg aaagcaccgc aaccgatatg
      241 cgcgaactct taaaagaaga gccctccatc gatttcggcg gcggcaacgg cacgtcccaa
      301 ttcctgacgc tgcgcggtat gggtcagaac tctgtcgaca tcaaggtgga caacgcctat
      361 tccgacagcc aaatccttta ccaccaaggc agatttattg tcgatcccgc tttggttaaa
      421 gtcgtttccg tacagaaagg cgcgggttcc gcctctgccg gtatcggcgc gaccaacggc
      481 gcgatcatcg ccaaaaccgt cgatgcccaa gacctgctca aaggcttgga taaaaactgg
      541 ggcgtgcgcc tcaacagcgg ctttgccggc aacgaaggcg taagctacgg cgcaagcgta
      601 ttcggaaaag agggcaactt cgacggtttg ttctcttaca accgcaacga tgaaaaagat
      661 tacgaagccg gcaaaggttt ccgcaatgtc aacggcggca aaaccgtacc gtacagcgcg
      721 ctggacaaac gcagctacct cgccaaaatc ggaacaacct tcggcgacgg cgaccaccgc
      781 atcgtattga gccatatgaa agaccaacac cggggcatcc gcactgtgcg tgaagagttt
      841 gccgtcggcg gcgaaaattc acggataact attaaacgcc aagcccctgc ctaccgcgaa
      901 accacacaat ccaacaccaa tttggcgtac accggcaaag atttgggctt tgtcgaaaaa
      961 ctggatgcca acgcctatgt gctggaaaaa aaacgctatt ccgccgatga caaagataac
     1021 ggctacgcag gcaatgtaaa aggccccaac catacccgaa tcgccactcg gggcatgaac
     1081 ttcaacttcg acagccgcct tgccgaacaa accctgttga aatacggcat caactaccgc
     1141 catcaggaaa tcaaaccgca agcgtttttg aacggcgaat ttaagatctc cgataaggaa
     1201 aaaggaccca acggaacaga agtcgacgta gatgtagcac aaaaagcaaa aaaccgtgca
     1261 aatgaaaaaa ttgtttacgc ctacaaactt tccaacccga ccaaaaccga taccggcgcg
     1321 tatatcgaag ccattcacga aattgacggc tttaccctga ccggcgggct gcgttacgac
     1381 cgcttcaagg tgaaaaccca cgacggcaaa accgtttcaa gcagcagcct caacccgagt
     1441 ttcggcgtga tttggcagcc gcgcgaacac tggagcttca gcgcgagcca caactacgcc
     1501 ggccgcagcc cgcgcctgta tgacgcgctg caaacccacg gcaagcgcgg catcatctcg
     1561 attgccgacg gcaccaaagc cgaacgcgcg cgcaataccg aaatcggctt caactacaac
     1621 gacggcacgt ttgccgcaaa cggcagctac ttccggcaga ccatcaaaga cgcgcttgcc
     1681 aatccgcaaa accgccacga ctccgtcgcc gtccgcgaag ccgtcaacgc cggctacatc
     1741 aaaaaccacg gttacgaatt gggcgcgtcc taccgcaccg gcggcctgac cgccaaagtc
     1801 ggcgtaagcc acagcaaacc gcgcttttac gatacgcaca aagacaagct gttgagcgcg
     1861 aaccctgaat ttggcgcaca aaccggccgc acttggacgg cctcccttgc ctaccgcttc
     1921 aaaaacccga atctggaaat cggctggcgc ggtcgttatg ttcaaaaagc cgtgggttcg
     1981 atattggcgg caggtcaaaa agaccgcgac ggcaaattgg aaaacgttgt acgccaaggt
     2041 ttcggtgtga acgatgtctt cgccaactgg aaaccgctgg gcaaagacac gctcaatgtt
     2101 aatctttcgg ttaacaacgt gttcgacaag ttctactatc cgcacagcca acgctggacc
     2161 aataccctgc cgggcgtggg acgtgatgta cgcttgggcg tgaactacaa gttctaaaac
     2221 gcacatcccg aaaaaatgcc gtctgaaagc ctttcagacg gcatctgtcc tgataatttg
     2281 atatatagtg gattaacaaa aaccggtacg gcgttgcccc gccccggctc aaagggaacg
     2341 attccctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctgcgg
     2401 cttcgccgcc ttgtcctgat ttaaatttaa tccactatag attatcattt atcctttcta
     2461 aagccgttcc ggtttgtccg accggcggct ttgccccaat atccccattt tggagacacc
     2521 ctatgttgcg tttgaccaca ttgaccctct gcaccgtcct cgccttgggc gcgtgttcgc
     2581 cgcaaaattc cgaccctgcc ccacaagcca aagaacaggc ggtttccgcc gcacaatccg
     2641 aaagcgcgtc cgttaccgtc aaaaccgcgc gcggcgacgt tcaaataccg caaaaccccg
     2701 aacgcatcgc cgtttacgat ttgggtatgc tcgacacctt gagcaaactg ggcgtgaaaa
     2761 ccggtttgtc cgtcgataaa aaccgcctgc cgtatttaga ggaatatttc aaaacgacaa
     2821 aacctgccgg aactttgttc gagccggatt acgaaacgct caatgcctac aagccgcagc
     2881 tcatcatcat cggcagccgc gccgccaagg cgtttgacaa attgaacgaa atcgcgccga
     2941 ccatcgagat gaccgccgac accgccaacc tcaaagaaag tgccaaagag cgcatcgacg
     3001 cactggcgca aatcttcggc aaacaggcgg aagccgacaa gctgaaggcg gaaatcgacg
     3061 cgtcttttga agccgcgaaa accgccgcgc aaggtaaggg caaaggtttg gtggttttgg
     3121 tcaacggcgg caagatgtcg gctttcggcc cgtcttcacg actgggcggc tggctgcaca
     3181 aagacatcgg cgttcccgct gtcgatgaat caattaaaga aggcagccac ggccagccta
     3241 tcagcttcga atacttgaaa gagaaaaatc ccgactggct gttcgtcctc gaccgcagcg
     3301 cggccatcgg cgaagagggt caggcggcga aagacgtgtt gaacaatccg ctggttgccg
     3361 aaacgaccgc atggaaaaaa ggacaggtcg tttacctcgt tcccgaaact tatttggcag
     3421 ccggcggcgc gcaagagctg ctgaacgcaa gcaaacaggt tgcggatgcg ttcaacgccg
     3481 cgaaataatg aaacggcggc attcgatgcc gtctgaaaca cggatgcaaa ccgcttcctg
     3541 tgtttcagac ggcattgccc gatacgggcg ttatcgcggg aatggcggca agcgttttga
     3601 ttggaacttg ccccatcctc tgcccgtccg gaggtttcaa acaaggcttt ccgctccgac
     3661 ggttcggact gccttgtttg aatctcctac gccgtaacgc ttttcccttt tgtttatgac
     3721 tgccaaacct ttttccctca acctggccaa cctcctgctg ccggcggtat tgtttgccgt
     3781 cagcctgtcg gtcggcgttg ccgatttccg ctggtcggat gtgttttcgc tgtccgacag
     3841 ccaacaggtc atgttcatca gccgcctgcc gcgcacgttt gcgattgtgt tgacgggcgc
     3901 gtcgatggcg gtggcgggga tgattatgca gattctgatg cgcaaccgtt ttgtcgagcc
     3961 ttctatggcg ggtgcgggcc aaagtgcggc tttgggtttg cttctgatgt ccctgctgct
     4021 gcctgccgcg ccgctgccgg tcaaaatgtc ggttgccgcc gttgccgcgc tgatcgggat
     4081 gttggtcttt atgctgctga tccgccgcct gccgccgacg gcgcaactga tggtgccgct
     4141 ggtggggatt attttcggcg gcgtggttga ggcggtggcg acgtttatcg cgtataaaaa
     4201 cgaaatgctg caaatgctcg gcgtgtggca gcagggcgac ttttcaagcg tgctgctggg
     4261 gcggtacgag ctgctttgga ttacgggcgg tttggcggtg tttgcctacc tgattgccga
     4321 ccggctgacg attttggggc tgggcgaaac ggtcagcgtg aatttgggtt tgaaccggac
     4381 ggcggtgttg tggtcgggtt tgattattgt ggcactgatt acatcgctgg tcattgtaac
     4441 ggtcggcaat attccgttta tcgggctggt cgtgccgaat atcgtcagcc gcctgatggg
     4501 cgacaggctg cgccaaagcc tgcctgcggt cgccctcttg ggcgcgtctt tggttttatt
     4561 gtgcgacatt atcggacgcg tgattgtgtt tccgtttgaa attccggtct ctacggtttt
     4621 tggtgtgttg ggtacggctt tgtttttgtg gcttttatta aggaaacctg cccatgccgt
     4681 ctgaaaaaaa tatcggtttt atggcaggaa gcagccgtcc gttgcgggtc gcctttgcgc
     4741 tgttgctggt ttcctgcatc ctgtttatga cggtcaacgt caaaggcgat tgggactttg
     4801 tcttgcacct gcgcctgacc aagcttgccg cgctgctgat ggtcgcctat gcggtcggcg
     4861 tgtccactca actcttccaa acgctgacca acaacccgat tctgacccct tcgattttgg
     4921 gtttcgattc gctgtatgtg tttttgcaga ccttgctggt gtttacgttc ggcggcgtgg
     4981 gctatacatc cctgccgttg acgggcaaat tcgacttcga actggtcatc atgatgggcg
     5041 gctcgctgct gctgttttac acgctcatcc gtcagggcgg gcgcgatttg ccgcgcatga
     5101 ttttaatcgg cgtgattttc gggattttgt tccgcagcct ttcctcgctg ctttcgcgca
     5161 tgatagaccc cgaagaattt accgccgcgc aggcgaatat gtttgccgga ttcaataccg
     5221 tccgcagcga gcttttgggc ataggcgcgc tggtcctgct cgtcagcgcg gcggtcgttt
     5281 ggcgcgaacg ctaccgctcg gacgtacacc ttttggggcg cgaccaagcc gtcaatttgg
     5341 gcatcagcta cacgcgcaac accttatgga tactgctttg gattgccgca ttggtggcga
     5401 cggcgaccgc cgtggtcggc ccggtaagct ttttcgggct tctcgccgcc tcgcttgcca
     5461 accacttttc cccgtccgtg cgccattccg tccgcctgcc gatgacggtt tgcgtcggcg
     5521 gcatcctctt ggtcggcgga caaaccgtat tcgaacactt cttgggcatg aaggcggtat
     5581 taagcgtggt ggtcgaattt gcgggcggac tcgttttcct ctatctcgtt ttaaaacaca
     5641 aaaaatgacg gatgccgtct gaacggcagc ccgccccgaa aggacaaacc atatgacgca
     5701 agaacgttta cccgaatttt tcgaccgcgc cccgacgctg accgtacaag acccgcttgc
     5761 cgcattcctc ggcgcggccg aaaacggcat cctcacttac cgctacgccg atgccgtgcg
     5821 cctgtgcgga cattcctgcc cgaccgtcgc gggcgcgtac ctgatggtta tcaaaggcct
     5881 gaaagcactt tacggcgaag agctgcccga acgcggcggc atcgaagccg ccatgcaggg
     5941 aacgcgcgac gaaggcacgg tcggcgtaac cgcgtccgtc gtccaactcc ttaccggcgc
     6001 agcccccgaa accggcttcg gaggcatcgg aatgcaggga cgcttcgccc gccgcaacct
     6061 cttatccttt ggtgcaggcg aaatcaacgg cacactgacc ctgcgccgca aagacaacgg
     6121 caaaaccgtt gccgtcagcc tcaacgccgc cctgcaaccc tttgcacctc aaatgcgcga
     6181 catcatgccc aaagccgtcg gcggcagcgc aagcgcaaaa gaactcgaac gcttcggaca
     6241 actttggcag gcacgcgtta aagcattttt gaccgaatcg gcggacgacc cgcagttcgt
     6301 catcgtccgc gaactgtgaa cgttcagacg gcattccgaa ctccaaatgc cgtccgaacc
     6361 cccgccaaac aacaacaaac ctacgcccga caagcatccg ccatgattac catccgcaac
     6421 gtcagctacc gcatcggcac acaccccatc ctcgacaacg tcagcctcga catccccgaa
     6481 ggcggcatta ccgcgctaat cggccccaac ggtgcgggca aatccaccct gttttccttt
     6541 atggcgcggc tgcgcccgct tgaaagcggc agcatcgcct accgaggcaa aaaccttgcc
     6601 ggcaccccca ccgccgaact cgcccgcacg ctctccatcc tcacccaaga aaacagcatc
     6661 atgagccgca tcaccgtgcg cgacctgctg atgttcggcc gttaccccta ccatcaaggc
     6721 agaccgactg ccgaatgccg ccgtatcgtt aacggtgcaa tcgaagaatt ccacctgcag
     6781 gacctctccg accgctacct gaccgaactc tccggcggac aacgccaacg cgccatgatt
     6841 gcgatggtgt tctgccaaag caccgactac gtccttttgg acgaaccgct gaacaacctc
     6901 gatatgtatc acgcccgctc gctcatgcag attctgcaaa agctcacgca cgaacacaaa
     6961 cgcaccaccg tcgccgtatt gcacgacatc aaccaggccg ccgcctacgc cgaccacgtc
     7021 gtcgccatga aaaacggcaa agccgccctg accggcacac ccgaagaagt gttcaccgta
     7081 cacaacatca aagaactgtt cgacatggac gtggacgtac tcgactacga aggcaaaaaa
     7141 ctcatcgtcc accacatctg aagctcaaaa aga
//
LOCUS       DAXFZR010000093         7088 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000093 DAXFZR010000000
VERSION     DAXFZR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.094"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(955..1638)
                     /locus_tag="ACFJAS_001877"
     CDS             complement(955..1638)
                     /locus_tag="ACFJAS_001877"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC3983046.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1787..4090
                     /gene="parC"
                     /locus_tag="ACFJAS_001878"
     CDS             1787..4090
                     /gene="parC"
                     /locus_tag="ACFJAS_001878"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC3983047.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRVVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNIFLGDENEKRKLIIKEMQANMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPISSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4110..5828
                     /locus_tag="ACFJAS_001879"
     CDS             4110..5828
                     /locus_tag="ACFJAS_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFC3983048.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWLIFFSTLNPQWQWQALRIPSFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVGSSCLLSYRRYPLLYASYASILLIFNALADSNINMYPLILDAK
                     TVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQEAV
                     VVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSRTFEHHIDTPELTA
                     RIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     DGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEALVE
                     SGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQMLL
                     NYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPSET
                     AAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRYRM
                     ERLNID"
     gene            5928..6680
                     /locus_tag="ACFJAS_001880"
     CDS             5928..6680
                     /locus_tag="ACFJAS_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3983049.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVILLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
     gene            complement(6858..>7088)
                     /locus_tag="ACFJAS_001881"
     CDS             complement(6858..>7088)
                     /locus_tag="ACFJAS_001881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983050.1"
                     /translation="SFPQKRESRSLGSGNLQKSSEKSEVLDSRFHGNDGISDGILPPR
                     HSRAGGNLVRSVSVIYDKFLLPFISGFPLSRE"
BASE COUNT         1707 a         2097 c         1745 g         1539 t
ORIGIN      
        1 tattttagta gttttaccat tagaaaaagg atatgaatgg aattgccttg aggtagaagt
       61 actatccgct gctcaatctc tagcaaatat ctattgcaac aaggttcgcc tcatagatgg
      121 tcatgtttgg gaggaataaa tgaaaaagcg tagttggtaa tttttagttg cgcagggtgg
      181 gcttcagccc accgtttttt cttcattttg ggttgttgat tggtgggcta aagcccaccc
      241 tacgattact aaaatcccgt cattcccgcg tagagcgcat cataataagg cgggaatcca
      301 gacctttcgg tgcgaaaact tatcggataa aacggtttcc cctgcatcct ggattcccac
      361 tttcgtggga atgacgggat ataggtttcc ctacggacgt gttcagattc ctgccttcgc
      421 gggaataacg gcggagcagt ttctgctttt ccaataaatg accgtaactt aaaatcccgt
      481 catccccgcg aaagcaaaaa tcctgcctgt cggatttcgg ttttttcggg tgtttcggga
      541 aactgatgaa tcgtcattcc cgcgcaggcg ggaatccaga acgcgggacg gcggcaatat
      601 tcaaaggttg tctgaaaatt cagaggttcc agattcccgc cttcgcggga atgacagcgg
      661 agcagtttct gcttttccga taaatgaccg caacctaaaa cccatccttc acacgaaaac
      721 agaaaaacaa aaacctaaaa tcccgtcatt cccgcgataa cagttgcgta attgcgtagg
      781 gtgggcttca gcccaccgtt tcttcatttt tttagtggac tgaagcccac cctgcggtta
      841 ctgtttcttc tttttcgatt attaattggc aggctgaagc cctccctacg tttaccgtca
      901 aaaatgccgt ctgaaatgct caagccctca gacggcattt tttatttcgc acccttacag
      961 gtaaaatttc tcgatgactt tcaggttgtc gtctaatttg tacaccagcg gctggccggt
     1021 cgggatttcc aagcccatga tgtcttcgtc ggaaatgccc tcgatgtgtt ttgccagcgc
     1081 gcgcagggag ttgccgtgcg ccgctaccaa gacgcgtttg ccgctcaaaa tcgcgggggc
     1141 gatttggtct tcccaaaacg gtaatacgcg ctccagcgtt actttcaggt tttcgccgtc
     1201 gggtacgaca tcggcaggca gatgggcata gcgacggtct ttgtgtgcgg aaaacgcgtc
     1261 gtctttgtcc aaaagcggcg gcagggtgtc gtagctgcgc cgccagatgc ggacttgctc
     1321 gtcgccgtat ttttcggcgg tttgtttttt gtccaaacct tgcagtcggc cgtagtggcg
     1381 ttcgttcagc ctccatgttt tgatttgcgg tacgaacagt tggtcggatt cttccaaaac
     1441 gatgttgcag gtcttaatcg cgcgggtcag gacggatgtg aaggcgatgt cgaactcata
     1501 gccgttttct ttcagttttt taccggcggc ggcggcctcg gcaagcccct gctcgctcag
     1561 cttcacgtcg cgccagcctg taaacaggtt tttcgcgttc cattcgcttt gtccgtggcg
     1621 gataaatacc aattccatat cgtctccaat gtgtgaaagt gggaaagctt tatttataac
     1681 atattttcac atttcccgta tttgattcag attcaggcac ggcgcccact atggtttgcc
     1741 attttgattt acaataatgt cctttgcttt acattccgca tacacaatga atacgcaacc
     1801 gcacgcttcc cataccgatt ccaacacgct gatgctcggc cgatacgccg aacgcgccta
     1861 tctcgaatac gccatgagcg tggtcaaagg ccgcgcgctg cctgaagttt cagacggcca
     1921 aaagcccgtg cagcggcgca ttttgtttgc catgcgcgat atgggtttga cggcgggggc
     1981 gaagccggtg aaatcggcgc gcgtggtcgg cgagattttg ggtaaatacc atccgcacgg
     2041 cgacagttcc gcctatgagg cgatggtgcg catggctcag gattttacct tgcgctatcc
     2101 cttaatcgac ggcatcggca acttcggttc gcgcgacggc gacggggcgg cggcgatgcg
     2161 ttacaccgaa gcgcggctga cgccgattgc ggaattgcta ttgtccgaaa tcaatcaggg
     2221 gacggtggat tttatgccga actacgacgg cgcgtttgac gagccgctgc accttcccgc
     2281 ccgcttgcct atggtgttgc tcaacggcgc gtcgggcatc gcggtgggta tggcgaccga
     2341 gattccgtcg cacaatttga acgaagtcac gcaggcggcg attgcactgt tgaagaaacc
     2401 gacgctggaa accgccgacc tgatgcaata tattcctgct cccgattttg ccggcggcgg
     2461 tcaaatcatc acgccggcgg acgaattgcg ccgtatttac gaaaccggca agggcagcgt
     2521 gcgcgtgcgt gcgcgttatg aaatcgagaa attggcgcgc ggacagtggc gcgtcatcgt
     2581 aaccgaactg ccgccgaacg ccaactccgc caaaatcctt gccgaaatcg aagagcaaac
     2641 caacccgaaa ccgaaagcgg gcaagaagca gctcaaccaa gaccggctta ataccaaaaa
     2701 gctgatgctg gatttaatcg accgcgtgcg cgacgagtcc gacggcgaac atcccgtgcg
     2761 ccttgtattt gaaccgaaat ccagccgcat cgataccgat accttcatca acacgctgat
     2821 ggcgcaaact tcgctggaag gcaatgtgtc catgaacttg gtgatgatgg gtttggacaa
     2881 ccgccccgcg cagaaaaacc tgaaaacgat tttgcaggaa tggctggatt tccgcgtcgt
     2941 taccgtaaca cgccgtctga aattccgttt aaaccaagtg gaaaaacggc tgcacatcct
     3001 cgaaggccgt ctgaaagtct ttctgcacat cgacgaagtg attaaagtca tccgcgaatc
     3061 ggacgacccg aaagccgatt tgatggcggc attcgggctg accgaaatcc aggccgaaga
     3121 cattttggaa atccgcctgc gtcagctggc gcgtttggaa ggtttcaaac tcgaaaaaga
     3181 attgaacgaa ttgcgcgaag aacaaggccg tctgaatatc tttttgggcg acgaaaacga
     3241 aaaacgcaag ctgattatca aagagatgca ggcgaacatg aagcagttcg gcgacgcgcg
     3301 ccgcacgctg gtggaagagg ccggacgcgc cgtgctgaca caaaccgccg ccgacgaacc
     3361 catcacgctg attttgtcgg aaaaaggctg gatacgcagc cgtgccggac ataatctcga
     3421 tttgagccaa accgcgttca aagaaggcga ccgcctcaaa caaacccttg aaggccgcac
     3481 tgttttaccc gtcgtcatcc tcgattcatc gggcagaacc tactcgatcg atgccgccga
     3541 aatccccggc ggacgcggcg acggcgtacc gatttcctcc ttaatcgagt tgcaaaacgg
     3601 cgcgaaaccc gtcgcgatgt tgacaggatt gccggaacaa cattatttat tatcaagcag
     3661 cggcggctac ggctttatcg ccaagctggg cgatatggtc ggacgcgtga aagcgggcaa
     3721 agtggtgatg accgcagaca gcggcgaaac cgtcctgccg ccggttgccg tctatgcctc
     3781 ctcgttcatc aaccccgact gcaaaatcat tgcagccacc agtcaaaacc gcgccctcgc
     3841 cttccccatc ggcgaattga aaattatggc gaaaggcaaa ggactgcaaa tcatcggatt
     3901 aaacgccggc gaatcgatga cgcataccgc cgtttcttcc gagccggaaa tcctgattga
     3961 aagcgaaggc aggcgcggcg cggcgcacaa agaccgcctc cccgtcgccc tgattgaggc
     4021 aaaacgcggc aaaaaaggca gactgttgcc catatcgggc agcctgaaac agctttcttc
     4081 ccccaaataa acccggttcc gcacatatta tggtgatttc caacccccgc gaacttgaaa
     4141 aactcaaaga ccggattccc aatctgatca acatcatccg cgtcgccatc gttttcccgc
     4201 tgatgattat gcacatcctc gggctggaaa ccggcagccg tgcgaacctg cacgcctcat
     4261 ggacggcgtg ggcgttttac ttgtggcttg ccattgcctg ctggctgatt ttcttttcca
     4321 ccctcaaccc gcaatggcaa tggcaggctt tgagaatacc gagtttcagc gccgtggccg
     4381 acatcacgat gatcggcgtg ctgacctacc tgttcggcgg catcgattcg gggttcggca
     4441 tcctgatcct gcccttcgtg ggcagctcct gcctactcag ttacaggcgt tatcccctgc
     4501 tctatgccag ctatgcatcc atcctgctga tattcaacgc ccttgcagac agcaatatca
     4561 atatgtatcc gctgattctg gatgcaaaaa ccgtcaccaa taccttcgtc gtcgtagccg
     4621 gctcctattt cgtcgctatg attgcctcgc tgtcggtcag atacatcgac cgtgccggca
     4681 aacttgccca tgaaaaccac gtcgcctacc gccgtatcag gggcttgaac caaatcgtgc
     4741 tcaaccgcgt tcaggaagcg gtcgtcgtca tcaacgtcga gcatcagacc atactgttca
     4801 ataaaaaggc aaaagatctg ctccccatgc ttgaaatcgg acagcatacc tccctgttcg
     4861 accctgtcgc catcttatgg gataaaacat cctcacgcac cttcgaacac catatcgaca
     4921 cgcccgaact gaccgcccgc atccgcgccg tgccgatgaa caaaaagcag aacaagctgc
     4981 tcatcctcta tatccgcccg caaagcgaaa ttcaggcgga agccctgtcc gtcaaacttg
     5041 ccgcgctcgg acaactgacc gccaaccttg cccacgaaat ccgcaacccg atgtccgcca
     5101 tccgccacgc cgacggcggc actttatttt tggacgaagt ggccgacctg cccctttcca
     5161 tgcaggtcaa actcttgcgc gcgattcaag aaaaagccgt gcgccgtatc ggcgacgcga
     5221 ccgagcagcc cgtcgatgtc cgcatcgtct gcgccaccca caaaaacctc gaagcccttg
     5281 tcgaaagcgg cgcattccgc caagacctgt attaccgtct caatgtcgtc agcctcaata
     5341 tgccgtccct gcgtgaaatg cgcgaaaatt tgaagctgcc caccccctac ctcctgtaca
     5401 aacacagcca caacaaccgg ccctacacac tctcccccgc cgcgcaacag atgctcctga
     5461 attacagtta tccgggcaat ttccgcgaac tcgaaaacat cctcgaacgc gccgtcgccc
     5521 tgtgcgtcgg atatacagtg caaatcgacg acctgcaaat ccaagatgtg caccacaaac
     5581 ccgtccggac ggaaaccgcc gtccccgttg ccgatacctt gccgtctgaa acagccgccg
     5641 caccgtcccg ccttctcccg ttcgaccccg ataccatgca gatacaggac tatctcgaca
     5701 aaatcgaacg cgacatcatc ggacaagtcc tcaaacaaac cgaaggcaac cgcacgcaag
     5761 ccgccaaacg cttgggcatc agcttccgct ctatgcgcta ccgtatggaa cgcctcaaca
     5821 tagactgaca aacggcatcc gcaccatctc cgcccacccg aaaaaatgcc gtctgaaacg
     5881 gcacggggaa gcgggttcgc cccacgaccg aacggacaca atacaccatg accgacatcc
     5941 tcattgacga taccgccacc gaagccgtcc gcaccctgat acgggcattc cccctcgtgc
     6001 ccgtttccca accgcccgaa caaggcagtt acctccttgc tgaacacgat accgtcagcc
     6061 tcaggcttgt cggggaaaaa agcaacgtca tcgtcgattt tacctccggc gcggcacaat
     6121 accggcgcac aaaaggcgga ggagaactca tcgccaaagc cgtcaaccac accgcgcacc
     6181 ccaccgtatg ggatgcaacc gcaggattgg ggcgcgacag cttcgtcctc gcctcgctcg
     6241 gactgaccgt taccgccttc gagcaacatc ccgccgtcgc ctgcctgctt tcagacggca
     6301 tccgccgcgc cctcctcaat cccgaaacgc aagacaccgc cgcgcgcatc aacctccatt
     6361 tcggcaatgc cgccgaacaa atgcctgccc ttgttaaaac acaaggcaaa cccgacatcg
     6421 tttatctcga cccgatgtat cccgaacgcc gcaaaagtgc cgccgtcaaa aaagaaatgg
     6481 cttatttcca ccggcttgtc ggcgaagcgc aagatgaggt catcctcctt cataccgcac
     6541 gccaaacggc aaaaaaacgc gtcgtcgtca aacgcccgcg cctcggcgaa caccttgccg
     6601 gacaagcccc cgcctaccaa tacaccggca aaagcacccg cttcgacgtt tacctgccct
     6661 acggagcgga caagggataa cgcccataaa acaagacacc gaaaaatttg ccgttcttat
     6721 gcaaacgaga aaccggtttt tgcgtttcga ctgttttgga taagtcatca caccttaaag
     6781 tttgtcattc ccacggaagt gggaatccga ttcgttcagt tttagttgtt ttcaagtttc
     6841 aggcaacttc caaaccgtca ttcccgcgaa agtgggaatc cggaaatgaa aggcaacagg
     6901 aatttatcgt aaatgactga aaccgaacgg actagattcc cgcctgcgcg ggaatgacgg
     6961 ggcgggagga tgccgtctga aattccgtca ttcccgtgaa aacgggaatc tagaacttct
     7021 gatttttcag acgacttttg aagattgccg ctacccaatg atctggattc ccgcttttgc
     7081 gggaatga
//
LOCUS       DAXFZR010000094         7022 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000094 DAXFZR010000000
VERSION     DAXFZR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.095"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..202
                     /locus_tag="ACFJAS_001882"
     CDS             <1..202
                     /locus_tag="ACFJAS_001882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983051.1"
                     /translation="LSKYILKNLITYMIAKVAACMPVIMAEIPFGRQSHRMSRHGRRC
                     ADAFGREGREIGSLEKGNGIRR"
     gene            complement(327..1133)
                     /locus_tag="ACFJAS_001883"
     CDS             complement(327..1133)
                     /locus_tag="ACFJAS_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC3983052.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQTVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(1134..1880)
                     /gene="pssA"
                     /locus_tag="ACFJAS_001884"
     CDS             complement(1134..1880)
                     /gene="pssA"
                     /locus_tag="ACFJAS_001884"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC3983053.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            2463..2945
                     /locus_tag="ACFJAS_001885"
     CDS             2463..2945
                     /locus_tag="ACFJAS_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC3983054.1"
                     /translation="MMKKILAVSALCLMTAAAQAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(2992..4203)
                     /locus_tag="ACFJAS_001886"
     CDS             complement(2992..4203)
                     /locus_tag="ACFJAS_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC3983055.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            4425..6863
                     /locus_tag="ACFJAS_001887"
     CDS             4425..6863
                     /locus_tag="ACFJAS_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC3983056.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTEAKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     FNGSRKILAHKDHL"
BASE COUNT         1764 a         1824 c         1973 g         1461 t
ORIGIN      
        1 attgtcaaaa tacattctaa aaaatttaat aacttatatg attgcaaagg ttgcggcttg
       61 tatgccggtc attatggcgg aaataccgtt tgggaggcag tcgcatcgca tgagtcggca
      121 cggcaggcga tgcgccgatg ccttcgggcg tgaaggcagg gaaatcggca gtttggaaaa
      181 aggcaatgga atccgcaggt aggtgtcgat tgggtggtga aaggcaataa gtcgggcgca
      241 cgctcagact gcctatcccc ttgtcggaac gggaggtagg gcagctttgt cctgactaat
      301 tgcttgtgca aacggtaaga aggtttttaa agcaggttgt acagcatttt tccggcaatc
      361 aaaagcaaca taatgccgaa ggattctttg agtttggcag aagaaagttt gtgggcggtt
      421 ttgacaccga gcggggcaaa ggcaatagtt gccgcgctga ggacggcgac ggcgggcagg
      481 taaaggaagc ccagcgaccc ttcgggcaat cctgcaatat tcagaccgtt gaccagatac
      541 gatattgcgc cggagagtgc aatcggccag gcaaggccgg atgatgtgcc gatggcttta
      601 tgggcgggga agccgcagtg gattaagaag gggacggaaa gtgaaccgcc gcctatgccg
      661 acccagctcg acattgcgcc gaacagtgtg gaaaccgcag tcagtccggg cagcccgggc
      721 agcgggcggg atgccgtctg acgaccggta tgcagtgttt tgaatgcgac ggcggttaaa
      781 aacaggatga agaaaatttg aagcccgaac gcggggatat attttgcgga gagtgcgcca
      841 gcgaatacgc cgaatatcat acccggcatc atcgcaaata tggttttcca gtcgaccgtc
      901 tgttttttgt gctgccccaa catactggaa aaggcggtga agaccatgac ggcgaaggat
      961 gtgccgacgg cgaggtgttg cgcgtaagga tgttgtgcca aaccctgcaa atcaagcacc
     1021 cataaaacga cagggacaat cagcgtaccg ccgcctacac cgaacaggcc ggcaataaaa
     1081 cctgccgcac tgcctacggc aagcaggatt aagataatgt cccaatgcca catttaatcc
     1141 gcctttctgt attttttcca aaatcggcgt gccgccataa tgtagccgga caggctgtat
     1201 ccgagaaaga acaggaaaag gacgagcgac ggttcccaag tgaccagaag cagcagtaag
     1261 acggcaagca gcattccgac aaaggggact tgtctgcgga tgttgatttc tttaaaactc
     1321 caaaaaggga tttggacaat catcgacagg ccggcaaaca gtgtgatgcc taatgcccac
     1381 cagtggacgg cggggaattt ttcgacgctg tggttgaccc aaatcagccc gacaatcagc
     1441 gcggcggcag tcggactggg tacgccgata aaccagcgtt tgtccacctt gccgatgagc
     1501 gtgttgaaca gcgcgaggcg tagggcggcg caggcgcagt agatgaaggc gacggaataa
     1561 ccgattttgc cgaactgcca aagctgccat ttgtaggcaa taagcgcggg ggcaacgccg
     1621 aagctgacca tgtcggcaag gctgtcgagc tgctccccga acgcgctttg gctgttggtc
     1681 agccgcgcca cgcgcccgtc cataccgtcc agcagcatag agatgaatac cgcgatggcg
     1741 gcggtttcat aacgtccgtg catggattgg gtaatggcgt aaaacgcgga aaacagcgcg
     1801 gcgatggtaa aggaattggg cagcaggtag atactgtttt gacgcaggga gcgttgcggg
     1861 gtgttggtcg gggtttccat agctgaagtg tctcacggac gtggagaatc gataggggat
     1921 tataacccaa accattatcg aacaatacgc cctttaggat gaagccctcg gaggcggttt
     1981 gtttttctga tggcggatgc tttgcggggc ggtttgactc tgcctgccac cgggtgtgtt
     2041 gtttcggatt ttgccggatg gatattgttg ccgggcattt ctgcgcttgc cggaaggtat
     2101 cggagggaag gcatcggaat atgcggcgcg ccgttcaaac cggggcggta tttccctatt
     2161 ttttgggggg tgcaggtttc aacaaattga aagatatttt ttgcatttcc gaccgtatgc
     2221 tgattttgaa gcgggataaa ttataggaca aggtttttcg cccgccgcaa aggttgcccg
     2281 caatgccgtc tgaaaacaac ggcttgccgc taaggcggca atatgaggat ttgcgctgcc
     2341 gaaaccggtt tgctacaacc cggcagtgaa atctgcaggg agggataggc cgataaggcg
     2401 ggacttatct tgttttttgt ttataatgca acagaatttg gaggaatcgt cgggaattta
     2461 tcatgatgaa aaagatactg gcagtatcgg cactatgcct gatgactgcg gcggcacagg
     2521 ctgccgatac ttacggctat ctcgccgttt ggcagaatcc gcaggatgca aacgatgttt
     2581 tgcaggttaa aaccacaaaa gaagattcgg cgaaaagcga agcgtttgcc gagttggaag
     2641 ccttttgcaa aggtcaggac acgcttgcgg gcattgccga agacgagccg accggatgcc
     2701 ggtcggtcgt gtcgctgaac aatacctgtg tctcgctggc atacccgaaa gccttgggcg
     2761 cgatgcgcgt tgaaaacgcc gtcgtgatta cttctccgcg ttttacgagc gttcatcagg
     2821 tcgcactcaa ccagtgcata aaaaaatacg gcgcacaggg acaatgcggc ttggaaacag
     2881 tgtattgcac gtcatcttct tattacggcg gggctgttcg ctccttaatc caacacctga
     2941 aataaaaaat gccgtctgaa ggatgttcgg acggcatttc catatcgacg gtcaggattc
     3001 tttcggatcg ggcagcaggc tgttcaacac aatggcgagt acggcgcaca agcccacgcc
     3061 gacaaagctg aagctgccca atttgagtgt cataccgccg atgcccgtgg tcagtaccga
     3121 gctgacgatg accaggtttt tcggcagcat caaatcgact ttggcatcaa tcagggtttt
     3181 gatgcctaaa gaagcaatcg tgccgaacag cagcagcata atgccgccca tcacgggcat
     3241 cggaatggaa gccaaaaacg cattgaattt gccgaaaaac gccatgcaga cggcaaaaac
     3301 cgccgcccaa gtcatgatga cggggttgct gtttttggta atcatcaccg cacccgttac
     3361 ttcgccgtag gttgtaaccg gcgggccgcc gatcagacct gcaacgcaca cgcccaagcc
     3421 gtcgcccgca agggttttgt ccaagcccgg gtcttttgtg tagtcttttc ccgttacatt
     3481 gccgattgcc atgatgccgc cgatgtgttc gatggcgggg gcgacggcaa cgggcagcat
     3541 aaacagtgcc gcctgccagt tgacctgagg cgtttcaaaa tggggaacgg cgaaccaggg
     3601 cgcgtgtgca atgcttgtcg tgtccaccag tcccatcagc agtgccaaaa cataacccga
     3661 agcgacaccg atcaagatgg gaatcagctt catcatcctg ctgccgaaaa ccgatacgat
     3721 ggcggtaacg gcaaaggtaa agccggaaag aatcagcgaa tcggtatagt cgatgacctg
     3781 tttgccgtca gcctgaccca ttgccatgct gcttgccgcc acggcaacag acagaccgat
     3841 gaccatgatg acagggccgg tgacgaccgg cggcagcagt ttgtgtaccg ccgccagtcc
     3901 gcgccaacgg atcagcgcgg caaacacaaa atacataaag ccggcggcaa acagcccgaa
     3961 catggtggaa ggcagccccc attcgccgac ggagtagata atcggcgcga taaaggcaaa
     4021 cgaagaaccg agaaaaatcg gtactttgcg tttggttgtg atttggaaca gcagcgttcc
     4081 caagcctgcg cccaaaagcg caagagccgg attcagaccg gtcagcaggg gaaccagcac
     4141 cattgcgccg aatgccacaa ataaaatctg cgcgccggaa acggcaagtt tcagttggtt
     4201 cataccatct tctttcaatc ggttcaaacg gcacggatta tactggtttg ccgaggggct
     4261 gcgtaaaaat acatacgtcg ggcaaagata tgccgtctga aaggcaacgt ctgtctgttg
     4321 caggatgaaa gcgggagaaa tggagtataa tccgacactt tccatattca atccgcaagc
     4381 cgtcccgaac ccttatttca gacggcattt tccggtaata cggcatgaca gaaaaatccc
     4441 ataaaaaaac agcaaaaggc agggcgggaa gcccgtcccc aacgtccgca cgcaacaaga
     4501 aagccgacaa cggcgcgcgg ggcaataaag tttccgagcg gctcaaggcg gtcaaagagc
     4561 tgcagaaaac tgaagccaaa aaggcgcgtc ccgaacatgt cgtcaacctg attgccgacg
     4621 cgctgtggct gatgggtttg gcggcaaccc tgtatttggc gatttccctg atcagtttcg
     4681 atatgggcga tccgtcttgg tcgcacagtt caccggttgt ggaagatgcc gccaattggg
     4741 gcggactgtt cggcgcgtat gttgccgatg tcggctatta tcttttcggc tggtcgttct
     4801 ggtggtggat agcggctgcc tgcgtcgtgc tgtataaaaa tttccgtctg cacgcaaaac
     4861 agacggaaaa cgaggcatac aaccacaaaa tcgctgccgc cgcgctgttt gtcctgacgg
     4921 tcttcagccc cgtcttggag tattttgtgc tgggcggaaa atatgccgac tccctgcctg
     4981 tcggagcagg cggtatggtc ggcatacgtg tcggcgcagt gtttgcgtgg ctgttgggga
     5041 aatcgggcag cctgctgatt atcctggttg ttctgctgtt gtcgttgtcc ctgctggtgc
     5101 agatttcatg gctggaattt ttgaacggtg cgggcagggc ggttcaaaac cgcctgagtg
     5161 ccttatccgg caaggtcatg gctttgagaa aacgccggcc gaataccaaa acagacggtg
     5221 tcgataccca aaatacacgg cgcatggtga aagaagccaa aaacatcact gccaaacccg
     5281 ttgccttgcc cgaaggcagc agcagcaacc gcaaatccgt cgcggtttcc gttgcgccgc
     5341 cgcctaaaat ccaaccttcg ctgtttgaag acaatgaagt acagcagaac ggcgaatacc
     5401 acaagcctac attgaaccta ttgcggattc ccgacagcga gccggtcagc atcaatcccg
     5461 ccgaattgga gcgcactgcc gaactaatcg aatccaaact ggcagaattc ggcatcggcg
     5521 tacaagtcgt atccgcgaca tccggccccg tcatcacgcg ctacgaaatc gaacccgcgc
     5581 aaggtgtcaa aggcagccaa attgttgccc tgtcgaaaga tttggcacgc tctatgtcgc
     5641 tgcaatccgt gcgtatcgtc gaaaccattg cgggcaaaaa cacgatgggc atcgagttgc
     5701 ccaacgacaa acgccaagac gtgatgttga gcgaaatctt gtcctcgccc gtgtttgccg
     5761 aagccaaatc caagctgacc gtcgcgctgg gcaaagacat cgccggtacg cccgtcgtcg
     5821 gcgacttggc gaaaatgccg caccttttgg tcgccggcat gaccggttcg ggcaaatccg
     5881 tcggcgtgaa cggcatgatt atgtctatgc ttttcaaagc tacgcccgaa gaagtccgct
     5941 tcattatgat agacccgaaa atgctcgagt tgagcattta cgacggcatt ccgcacctac
     6001 tctgtcccgt cgttaccgat atgcgcgaag cagggcaggc gttgaactgg tgcgtcgccg
     6061 aaatggaaaa acgctaccgc ctgctctccc acgccggtgt acgcaatctg gaaggcttca
     6121 accaaaaagt cgaacaagcc aaagcggcag gcaagccgct gctcaatccg ttcagcctga
     6181 acctcgacga acctgagcca ctggaaaaac tgccgatgat cgtggtcgtt atcgacgaac
     6241 ttgccgacct gatgatgacc gaacgcaaag ctgtcgagca gcaaatcgcc cgcctcgccc
     6301 aaaaagcgcg cgccgccggc atacacatga ttgtcgccac ccaacgccca agcgtcgatg
     6361 tcgtaaccgg tctgattaaa gccaacatcc cgacgcgtat ggcgtttacc gtgcaaagca
     6421 aaatcgacag ccgtaccatc ctcgaccaaa tgggcgcgga cgaactgctc aaatatggcg
     6481 attcgctgtt cctgcaaccg ggcagtgccg aaccgacccg cctgcaaggc acgtttgttt
     6541 cagacggcga agtgcatcaa gtcgtcaact acgtcaaatc gcaagcccca gccgactata
     6601 ttgaaggtct gctcagcggc gaagccgcgc tggaaactgc caatatcgtt aatccgaatg
     6661 cagacagcga cgaattattc gatcaggcag tcgcctatgt tttggaaagc aaaaaaacct
     6721 ccatttcgtc tttgcagcgg cagttgcgta tcggctataa ccgcgcggca aacctgatgg
     6781 aggcactgga aaatgcgggt atcgtttctc cctccgactt caacggcagc cgtaaaattt
     6841 tggcgcacaa ggaccattta tagcccgtat cgcaaaatgc cgtctgaacg gcggaattgg
     6901 cgtttcagac ggcatgaggc ctttgcaaaa aagcccttcc ttcgacatcc gaaacccaaa
     6961 cacaggtttt cggctgtttt cgccccaaat acctcctaat tttacccgaa tatcccctta
     7021 at
//
LOCUS       DAXFZR010000095         6894 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000095 DAXFZR010000000
VERSION     DAXFZR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.096"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            69..218
                     /locus_tag="ACFJAS_001888"
     CDS             69..218
                     /locus_tag="ACFJAS_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983057.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            248..3295
                     /locus_tag="ACFJAS_001889"
     CDS             248..3295
                     /locus_tag="ACFJAS_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFC3983058.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQSINSQVRQVTSSETEYLAVQQQLLDTANRTSASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGARQQAAALFQLSQALGSGVLQGDE
                     FKSISEAAPILLDTIAEYMGKSRAEIKKLGSEGKLTADVIFKAISGASEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLA
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYRIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGRNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVEESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(3355..3834)
                     /locus_tag="ACFJAS_001890"
     CDS             complement(3355..3834)
                     /locus_tag="ACFJAS_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC3983059.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(4176..5165)
                     /locus_tag="ACFJAS_001891"
     CDS             complement(4176..5165)
                     /locus_tag="ACFJAS_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3983060.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            complement(5425..5613)
                     /locus_tag="ACFJAS_001892"
     CDS             complement(5425..5613)
                     /locus_tag="ACFJAS_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983061.1"
                     /translation="MADGRKGFFDAIIKINIFFFPVKTPTLGAANPIAGGSTRFRFGA
                     EQMAVLYVPFCVLKHIGR"
     gene            5854..6888
                     /gene="purM"
                     /locus_tag="ACFJAS_001893"
     CDS             5854..6888
                     /gene="purM"
                     /locus_tag="ACFJAS_001893"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC3983062.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGLSVGAGDMVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
BASE COUNT         1721 a         1671 c         1950 g         1552 t
ORIGIN      
        1 cctgcgcgtc aaaccgcctt cgtgtaaagc ggttgcaaaa aagcctttta ttttgacagg
       61 gtgcagagat gaacgccgaa ggattttgtg aaaaacaaac cgcctgccgg ccggacgggc
      121 gccgaaatgc gggcgatcgt gccgatttga aggcctttga gtttgccgga cgggaattgg
      181 cgaattatcg ggaaatgttg aaacgttatg ccgtctgaaa agacggcttt tttatttggg
      241 gctgtaaatg agcgatttgg aagccaaagt caggataacg gtagaaaacc atacgaagca
      301 gggttttgat tcggccgccg ccgatgctga taaggcggcg gaaaagatgc gcagcagcgg
      361 cgataatgcc gcaaagggct ttaaagctgc aatcgacagt atgcacgaga ccatgcgcaa
      421 tttgcacgcc gatgtgaaag cgggatttga agcggcgggc aatcaggcgc aacaggcgtc
      481 tgaaaaggtt agggccgaag tgggtaaaat cggctcaggc ttatcggggc tgaccaaact
      541 gctggcagga ttagcaaccg cagacttcgc caaatcggtg cttgataccg ccgatgcgat
      601 gcagtcgata aacagccaag tccgacaagt cacgtcgtct gaaacggagt atctggccgt
      661 gcagcagcag cttctcgata cggcaaaccg taccagtgca tcgcttgaat caacggcaaa
      721 cctgtacgtt tccacaagcc gcgcgttgaa agactacggc tacacgcagc aggaaatttt
      781 aaaattcacc gaggcaacca acaacgcgat gaccatcggc ggcgttggcg cgcgacaaca
      841 ggccgccgcg ctgttccagt tatcgcaggc tttgggcagc ggcgtattgc agggcgatga
      901 atttaaatcc atttccgaag ccgcacctat tttgctggat accattgcgg aatatatggg
      961 caaatcccgc gctgaaatca aaaagctggg cagcgaaggg aaattgacgg cggatgtgat
     1021 tttcaaagcc atttccggcg cgtcggagaa gttcggggag caggcggcca aaatgcccgt
     1081 gacgatgggg caggctttga cggtgttctc gaacaactgg caaagcatgg tttcaaaact
     1141 gctgaacgac agcggcacaa tgtcggggat tgccgccgtt attaaactga ttgcggataa
     1201 ccttaatttg gttgttccga tcgtcgcagg ttttgccgtt gccgttgccg ccgccgttgc
     1261 accgacactg gctttgaatc ttgcactgct ggcaaacccg ttcgggatta ttgccgtcgc
     1321 aatcggcacg gttgtcgggc tgattgcgaa attcggcgat gaaatcgatg ttttcggcgg
     1381 cggctggtcg aatctttccg atgtgattcg ggcggtttgg caaatcatca cggaaaccgt
     1441 cggggaagcg gtgggaaccg tcaaatcgtg gtttgacggg ctgacaggcc ggctgaacga
     1501 gggcgcgggc ggctggccgt cgttgttcgg gcgcgtgatg agcgtcatct caagcgcaat
     1561 cggcgcgtat gtgaacgttt atatcaacat attcgcaacc ggctggatgc tgattaaaga
     1621 agccgcaaac gatatgccgc aattcttcgc caatctcggc aaggccatag gcaacgtgtt
     1681 tatttccgcg atcgaacgga tgataaacaa agcggtaggc atgattaaca gcatgattga
     1741 ctttgccaac aaagccgcgt cgatggccgg gatatcgggc attggaaagc tgaacaaggt
     1801 tcggatgggg cggatggacg acggcgggct tggcgagcgt attcatgaca gcttgacgaa
     1861 agaccgcgcc ggcgcaatgg caaatgccgt ccgcgaacgg gccgccgata ttcacgaagc
     1921 cgatgccctc aagggacgcg gcggcggagg acacgccaaa accgcccgga aaaagccggg
     1981 cgcaaatcag ggcggcggca aagggggcaa atcccgttcg ggcggtccgg gcgcggccaa
     2041 agacccgatg caggcttggg aaggggaaat caaagcccga aaactcgcac accgcgaaat
     2101 gcagcgcgaa acgctcgcac accaagaatg ggatttggcg cgtgaggcgg aatattggcg
     2161 ggcgaagctg gcaacggtgg acgccaacgg caaaacgggc gtgaaaatcc gcgaaaaaat
     2221 cctgacgctt gaagaccaat tatcgaagca gtcaaccgaa gccaaaatga atcaggcggc
     2281 cgaatgggag aaattggaca agcacaagct ggagatggag aaagacgcgg cagaccaagc
     2341 cctggcagac ggacgtattt cgcaactcga acgcctggac ttggaaatcg agtttgaaaa
     2401 ccgacgttac cggattgcct atgacgcatt gcaggaacgg atcgcacttg ccgaacaaga
     2461 cccggcatac agtcaggcgg caatcggcaa gctcaaggcc caaatgggcg aattggggcg
     2521 ggggcacgaa cggacgcagg cgaagaacga gggcaaacgc gaaaaccaac gccgcaaaga
     2581 cgcgcccaac gtcatggaaa tgctgcaaga cggcggcagg aacgtttggc aggaagcaca
     2641 gcagcagatg gggcaggcgt tcaccgccat gctggcgagg gcgcagaatt tcaggcaggc
     2701 gatgaacggc tttttcaaga gtatggggca aacctttatt caggaaatgg ttacgaaacc
     2761 tttgacgggc ttgatgcggc gcatggttga ggaatcggcc atttacaaga tgattttcgg
     2821 aactaaggaa acgctggaga cagcggcggc agccaagacg gcggcaacta aggcaaccga
     2881 gacaacggcg gttgtcagta gtaatgctgt tcaagcggct tcaggcgcgg cggcttccca
     2941 ggcgcatatc ccatatgtcg gcccgattct tgccgttgcg gcaatggcgg caatgatgtc
     3001 ggcagtgatg gggttgatgg gcggcggcgg cggttctcaa acgaccacaa ccacgacgcg
     3061 cattccctcg gcggcaggcg gctgggacat cccggcaggc atcaacccgc tgacccaact
     3121 gcacgaaaac gagatggtcc tgcctgccga acacgcgcaa accatccgtg aaatggcgga
     3181 tcagcaggga ggcggcagca ccgtcatcat caattcgaca ggcggcgact tcatccataa
     3241 aaacgatttg gcgaagctgt tgaaacagat gaaacgggac tttaaatttg tctgagaaaa
     3301 tgccgtccga aaggttcgga cggcctatgt tgcggcgtac gtcaagctat taagctattt
     3361 ggttttcaga ggctttttct tccatttccg ggctacggct tcaaattctt gaaaggccgt
     3421 cggcgcgctc ctgatacggc gtatttcaaa aatgaagctg cttaggttgt cccagtcgcg
     3481 taacagcatg gagtattcga aatctttata cattttctcg tgcagcgccc cgcccaatac
     3541 gcccgcacag acaaattcgc gctggtttaa aactgtcagt atggcttcac ggtctttctt
     3601 cttgtccggg gtatctgatg tataggtggc gagtatgcat ccgtctgttt ttgccagccc
     3661 gtttactatg gttatggctt cttgaagggc ggcattgtta cgttcggcca tgatcatgtc
     3721 gatggtggcg cgctttttgg cgttttccgt attcctcatg atgccgtaag cggcaacaaa
     3781 aacgccgata acagtcaata tcggcgttgc tatctgtatt aggttgtcag tcattgccgt
     3841 cccagccgtc cgaaaggcgg aaatcacggc tttccgaata aatatgcccc attgtcatcc
     3901 ccttaagctg atgttgagaa taaatccttg cgtcggaata tacggcattg cctgactatt
     3961 ttcaagattc cgcaccccgc ggaccggact atacaaaaaa accgccttcg tgtaaagcgg
     4021 ttgaagagag cctttcaata aaaatgccgt ccgaagggct ttcggacggc attttttata
     4081 gattcttttt taatgaacct ttgaaaccga agataattaa ttttcctctt tattttcaat
     4141 acgcagtgtg ccctatgttt ttaaataaaa atattttaat ccaacatttt tgcaatatcc
     4201 gccatgtcgg ggcggtagta aacattgagt aaaatcctta aatcccgatg cccgctgatt
     4261 tttgccaaga cctccacagg cacttttttt gataggcgcg tgagggcttc gcggcgggtg
     4321 tcgtgaaaat gcaggccttg tatgccgcaa ttatcccgcg ctcgcctgaa taatacatca
     4381 aggcttttgg catccaggct aaacacccat gtgtcatcaa ttccgcgcag ttgctctatt
     4441 agttcggcgg ctcgcaagga taatggcacg tcacggctgt cgccgttttt agttattggg
     4501 aggtgggcaa tacgacgccg catattcaca tctgcccatt ttaggccgca gatttcgcct
     4561 gcccgcatgg cggtttcgat ggcaaatagg acggcaacgg caacccgttg caccgccatt
     4621 ttcggtttct cattaggccg gtataggagg gcggcgcaaa tatcggcaat ttcctgctcg
     4681 gttggccgtc ttgtccttgc ccggcttttt ttcggcttgc tgattttgcg tacagggttt
     4741 tcgcgaagaa gtccccactc tttcatggcg tgttcgcaga cggcggataa agtggttaat
     4801 tcgcgtccga cgctggtggg ggatacctcc tgcaaccgtt gatctcgcca atcggcgaag
     4861 tcttgagggc gcaggtcggc aagccgcacc tttgccaaag gtgtttttag tgcgcgtcca
     4921 atgcggtatg attcttcccg tgcgccccgc tttgatggtg taacctcgga taaataccgc
     4981 cggataaggt cggcaaagat agtatttcgc ggggcattgc cttggatgcc gtccataatt
     5041 tgcgactcta ttcgcgccgc ccacgccttc gcgtctgatg cccgctcaaa tgtggcggaa
     5101 cgggaaacgc ctttcatacg gatttgtacg cgccatttgc cgttacgttg agtgatggtt
     5161 gccatattta ttcgggtgca attcgggtgc aaatataata gagcaaaaca gatataaagc
     5221 ggggcaaaat agggctatat taaatttaat gctttgatat gtagggataa atagatttaa
     5281 aaagacgaaa gaagtgcgga cgtagaataa gcgtagatgc gctccgtccg caccaataaa
     5341 aatatataaa tcatattgtt aatgattatt gggacaattc ggtggcacaa aatataagct
     5401 gtctgctttt cagcgggcgg ctttttatct gcctatgtgt ttcaacacac aaaacggcac
     5461 ataaagcacc gccatttgtt ccgcaccgaa tcggaagcgg gtgcttccgc ccgcaatcgg
     5521 attcgccgcc cctaatgtcg gggttttaac cggaaagaag aaaatattga ttttgatgat
     5581 ggcatcaaaa aaacccttcc gcccgtctgc caaaaggaat ggttaagaaa agcaatgcgt
     5641 cttttgatgg aacgcgatat ataagaaatt gaggggaagg gcaggcgggt ttctgcccgt
     5701 taatcctgcg gacggccccg ccaaacccga cacgcgcggg catatggcgg attgtttaca
     5761 atattgtcta aaaatctgaa cctgcaaacg cgaaataagg tagaatacgc cccgttattt
     5821 taccgtccaa tccccattta ccaaggaaaa acgatgagta cttcattgag ttaccgcgat
     5881 gcgggcgtcg gtatcgacgc aggcgaccaa ctggtcgaaa aaatcaaacc gtttgccaaa
     5941 cgcaccatgc gtccggaagt attgggggat ttgggcggtt ttggcgcatt ggtcgaaatc
     6001 ggcaaaaaat atcaaaaccc cgtattggtc agcggtacgg acggcgtggg taccaagctc
     6061 aaacttgcct tcgattggga caaacacgat acggtgggca tcgaccttgt tgcaatgagt
     6121 gtcaacgata ttttggttca aggagctgaa ccattgtttt tcttggatta ttttgcctgc
     6181 ggtaaattgg atgttccgcg tgcgaccgat gtcatcaaag gcattgccca aggttgcgaa
     6241 gaatccggct gcgccctgat tggcggggaa actgccgaaa tgccgggtat gtatcccgtt
     6301 ggtgaatacg atttggcggg ttttgccgtc ggcgtggtgg aaaaagagaa tgtcattacc
     6361 ggcctcagcg tcggcgcggg cgatatggta ttgggtttgg cttccaacgg cgcacattca
     6421 aacggctatt cccttatccg taaaatcatc gaacgcgaca atcccgatct ggatgccgag
     6481 tttgataatg gcaaaacttt acgtgaagcc gttattgcgc cgacacgtct gtatgtgaaa
     6541 ccgattcttg ccgctttgga aaaatttacc attaaaggta tggcgcacat taccggcggc
     6601 ggcattaccg aaaacgtgcc gcgcgtgttg cctaaaaaca cggttgccca aatcgatgcc
     6661 gaatcgtggg aattgcccaa gctcttccaa tggcttcaaa aggcgggcaa tgtggaaacc
     6721 caagaaatgt accggacctt taactgcggc atcggtatgg tcgtcatcgt tgctgccgaa
     6781 gatgccgatg cggttcggtc attcctgagc gggcagggcg agacagtgta ccgtttgggt
     6841 tgcatccgag agcgtcaagg gaacgagcat caaacccaag ttgcctgact tcca
//
LOCUS       DAXFZR010000096         6818 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000096 DAXFZR010000000
VERSION     DAXFZR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.097"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..408
                     /locus_tag="ACFJAS_001894"
     CDS             <1..408
                     /locus_tag="ACFJAS_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983063.1"
                     /translation="CQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKATHGPSVKNAV
                     YAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQYSIFEYEVEH
                     PENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            426..2210
                     /gene="lpdA"
                     /locus_tag="ACFJAS_001895"
     CDS             426..2210
                     /gene="lpdA"
                     /locus_tag="ACFJAS_001895"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC3983064.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(2634..4184)
                     /locus_tag="ACFJAS_001896"
     CDS             complement(2634..4184)
                     /locus_tag="ACFJAS_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC3983065.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPSFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4309..6554)
                     /locus_tag="ACFJAS_001897"
                     /pseudo
     CDS             complement(4309..6554)
                     /locus_tag="ACFJAS_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(6729..>6818)
                     /locus_tag="ACFJAS_001898"
     CDS             complement(6729..>6818)
                     /locus_tag="ACFJAS_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3983066.1"
                     /translation="PPFLRECEFRLNFGTPSRQLKILRDRCGI"
BASE COUNT         1659 a         1704 c         1685 g         1770 t
ORIGIN      
        1 tgccaacaag atgagcaaaa taatggacag ttaaaaccta aaggtaataa agctgaagtt
       61 gcaattcgtt atgatggtaa gtttaaatat gatggtaaag ctacacatgg tccaagtgtg
      121 aagaatgcag tttacgccca tcaaattgaa acagatctat atgacggatg ttatatatct
      181 acgacaacag acaaggaaat tgccaagaaa tttgcaacaa gctccggcat cgaaaatggc
      241 tatatatatg ttttaaatag agatttgttt ggtcaatatt ctatttttga atatgaggtt
      301 gaacatccag aaaacccaga tgagaaggaa gtaacaatca gagctgaaga ttgtggctgt
      361 attcctgaag aagtgattat tgctaaagag ttgatagaaa ttaactaagt tgaaaggtca
      421 atataatggc tttagttgaa ttgaaagtgc ccgacattgg cggacacgaa aatgtagata
      481 ttatcgcggt tgaagtgaac gttggcgaca ccatcgccgt tgacgacacc ctgattacct
      541 tggaaaccga caaagcgacg atggacgtgc ctgccgaagt tgcgggcgtg atcaaagaag
      601 tgaaagtcaa agtcggcgac aaaatctccg aaggcggttt gattgtcgtc gtcgaagccg
      661 aaggtgcggc tgccgcccct aaagccgaag cggctgccgc cccggcgcaa gaagcaccca
      721 aagctgccgc tcctgctccg caagccgcgc aattcggcgg tgctgccgat gccgagtacg
      781 acgtggtcgt attgggcggc ggtcccggcg gttactccgc tgcatttgcc gctgccgatg
      841 aaggcttgaa agtcgccatc gtcgagcgtt acaaaacttt gggcggcgtt tgcctgaacg
      901 tcggctgtat cccttccaaa gccttgttgc acaatgccgc cgttatcgac gaagtgcgcc
      961 acttggctgc caacggtatc aaataccccg aaccggaact cgacatcgat atgcttcgcg
     1021 cctacaaaga cggcgtggtt tcccgcctca cgggtggttt ggcaggtatg gcgaaaagcc
     1081 gtaaagtgga cgttatccaa ggcgacgggc aattcttgga tcctcaccac ttggaagtgt
     1141 cgctgactgc cggcgacgcg tacgaacagg cagcccctac cggcgagaaa aaaatcgttg
     1201 ccttcaaaaa ctgtatcatt gcagcaggca gccgcgtaac caaactgcct ttcattcctg
     1261 aagatccgcg catcatcgat tccagcggcg cactggcact gaaagaagta ccgggcaaac
     1321 tgctgattat cggcggcggc attatcggcc tcgagatggg tacggtttac agcacgctgg
     1381 gttctcgcct ggatgtggtt gaaatgatgg acggcctgat gcaaggcgca gaccgcgact
     1441 tggttaaagt atggcaaaaa caaaacgaat accgttttga caacattatg gtcaacacca
     1501 aaaccgttgc agtcgagccg aaagaagacg gcgtttacgt tacctttgaa ggtgcaaatg
     1561 cacctaaaga gccgcaacgt tacgatgccg tactggtcgc ggcaggccgt gcgcctaacg
     1621 gcaaactcat cagcgcagaa aaagccggtg ttgccgttac cgatcgtggc ttcatcgaag
     1681 ttgacaaaca aatgcgcacc aacgtaccgc acatctacgc tatcggcgat atcgtcggtc
     1741 aaccgatgtt ggcacacaaa gccgttcacg aaggccacgt tgccgctgaa aactgtgccg
     1801 gccacaaagc ctacttcgac gcacgcgtta ttcccggcgt tgcctacact tcccccgaag
     1861 tggcatgggt cggtgaaacc gaattgtctg ccaaagcatc aggccgcaaa atcaccaaag
     1921 ccaacttccc atgggcggct tccggtcgtg cgattgccaa cggttgcgac aacggcttta
     1981 ccaagctgat tttcgatgcc gaaaccggcc gcatcatcgg cggcggcatt gtcggtccga
     2041 acggtggcga catgatcggc gaagtctgcc ttgccatcga aatgggctgc gacgcggcag
     2101 acatcggcaa aaccatccac ccgcacccga ccttgggcga atccatcggt atggctgcgg
     2161 aagtggcttt gggtgtatgt accgacctgc ctcctcaaaa gaaaaaataa acctgatgct
     2221 ctaagcagcc gataaggttt atccgaacaa atgccgtctg aacccttcag gcggcatttt
     2281 tatttggctg tgttttaacc atctgtcaat tttaactggt tgcaagggaa aagacgatta
     2341 ttttccagtt aggcagacac ctacactatt gaataccttg aagctaagca tgcctatcaa
     2401 tactatatta aaatacagcc ttttattttt cctccatgct ttccaataac agatttcaac
     2461 tggatttcaa tagttcccct atcccgtccg aattccatat ctaaacattg gaagcgtgtc
     2521 ctgatatcgg attagagaca atcaattgtt tttgaaacat gaaaatccat cctatttcga
     2581 gaccgacaaa aatgccgtct gaacatttca gacggcattt ggcataaacg gcattaatgc
     2641 ggctggccgg aaaccatatc tccctcgtca aaatcaggat cgggttcatt ttgcagcgtt
     2701 ttcccgttta acttcagggc gttgttttta agggaaatga ccgtatcaat ctgattaccg
     2761 tctaaagtaa gatatttttc ccttgccata ctttggaccg tactgtccac catcaggcgc
     2821 aatgtttcat taatatcggc aatgcttgct ctggcttccg cctcatcttc ggcatttaca
     2881 ctgaaaatat ttccagcctg acttaccgcc aaatcttcca acattttttg aggaatactc
     2941 attctgatgt ttgcctcggt tttctttaac atcagtccca attggttcaa atcttccttc
     3001 ttcatgcctt taaacatgat ttttccgccc acatcaattt ttccctgagg cagggtgaaa
     3061 cggaaaattt tgatatttag taccgggtca tgggtaaata atccggaagc atcgcctttg
     3121 actgccgcaa tcaaatcatt gcggatttgt tcctcagtca tttttttggc agaaatttgt
     3181 gcaaacttgc gtttcaatac ggttaaggca gaagcatcga ggtgttcggc agcgatatgg
     3241 atgtccagcg ggccgtattt ttcatcgccg tacaccaacg tatcgaaacg gaaccgccct
     3301 tcgctgtcga taaacgcgcc cgattccccg gtcttggttg aaaaagccag cttgccgact
     3361 tcgattttgg aaggtgcgat gctgccgttg ggattgataa acgcgccgat ctgcaaatcg
     3421 gtaacgaggt tgaccagttc gttcaatttg acgttgtaat cgacaccctc tttccattcg
     3481 agcgaaaatt tttccaaagt cagattgctg ctgcccaaag caagcggatt gatgccgtct
     3541 gaagtttccg aatcgaaatg cgctttttca aacgcggcat cgcctttgtc tgccagcttg
     3601 attttgaaca agggcgcatc atagctgttg cggtagcttt tgaaaccttt ttgataaacc
     3661 gtttcccccg tcaggccttc ccagtgcagc ctgatgcccg acagttcttc ataatcgaaa
     3721 gagggaacac tgacttccat tttaccgctg ccgttgaaat aaacggtatt ggcaagggaa
     3781 accggaactt gtttcccaaa aaagcgttcc aaaacttttt ccgtttcagg cgcgtatttg
     3841 aactcggttt caatgtgcgc ctgcgtgccg aatccgccgg cgaaagggcc gtgcgtgata
     3901 tggtttacca gcgtaaccgg ctgttccaac actattttca agttatccgg caggtatttc
     3961 tgcgcattat gcagcaactc gggtttcaga cggatgaccg tcgtttccgt agaggtaaac
     4021 cagcctcgat catactggtg cgattcgacg gtcaaaaagc ccgttttctg caatattttt
     4081 tgctgctgcg tcagactttc ttctgctttg acacccaaat aataaggtgt acccaaagca
     4141 acgccgagca atactgccgc aactgaaatc aaaggttttt tcatcacttc aaacaagcag
     4201 gtttcaaagg tactagaata gcattattta agcatatccc gccatatttc tttaaaagaa
     4261 atgccgtctg aaacctgttc ggacggcatt ttcggaaacg gaggcagctt agaaatccaa
     4321 ttccgccttc agccagtaag tacgcggcag accgacgacg gcgaaactac ggtcaaattg
     4381 tccgcgctgt acctgccagt agttttttgt tgaacaggtt ttctaccgag ctgctgacgg
     4441 tcagggtgtt tttgcccagc ttggttttgt agcgcgcgcc tacgtcaacc aaggtatagg
     4501 acgggaaggc gtattgtttt tgcgtgtctt gataagactt gccgaaatac gaaacattgc
     4561 cgttcaagct caaacctttg gcaaacggtg tatcccgttc aacgcctgtt ttgacaatca
     4621 cgcgcgggtt ggcgacttgt acgccgttaa ccagcatatc gcgcgcattc ggataattct
     4681 tcacggacga ttgcagatac atcacgccga aagtcgggcg cagggttttg ttcaacaggt
     4741 tggcataggc attgaactca ataccgcgat tgcgttccat accttgctcg tcgccggctg
     4801 cgcctcctgc tgctttatat gctgcaaaat cagtaccttt tttggtattg ccgcgccagt
     4861 agcccgggcg tttgatttgg aacgcgttca acgtggttac aaaatcgccc cagtttttac
     4921 gcacgccgat ttcaaactgg cggctgacgc gcggcttcgc cattgtcgtt tcgccggaat
     4981 catcggtttt gatatcggca cgctccaagt cttccatata gttgccgcac acgaccaaat
     5041 caggttgcgg cacccacgcc gccatcagca tcgggctgaa acgtttggca tcgccgctct
     5101 gtgatttctt cttatcagtg tattcaaccg cttggaaacg tccgcccaaa gtcagggggt
     5161 atttgttatc aacgaagccc aaagtatcgg acaaagccaa gctgttgact ttgatattgg
     5221 catccaagtt ggccgagttc gcccaagaat tgggatagtc ggctttgaac gatgccaatt
     5281 gattcgcaat attttcgttt gccttcactt ctatcttgct gttgccgttc ccgctgccgt
     5341 ttatggtttt ccgctggcgg ataacgcggt cgaaagccgt actccagtta tgcgtgaccg
     5401 ggccggtttc aaattcgccg cgtgcggtca ggttcatact caaagtacgg aaatattggt
     5461 cggtcaggcg cgccgtgccg gtgtggtatt ggttggcagc catgcaggtt ttggtttggc
     5521 tgctcgcacc gcttgtaccg caaacggttg gggaaatcag cgtaccgtaa taacgcgctt
     5581 tgttgtagcc gataccgccg gtaatttagg tattgtcaaa cgcatcccat tcaaacgtca
     5641 gcatattggt ttcgccgacg gtattttgcc agttccaaga aggcagcagg ttgattttgc
     5701 cgtcgggcgc gtcgaacaag cgtccgccgg cgttttggat atccttcata cgcgcgcggc
     5761 cgccgttgat tttgcgtttc gcgtagatgg agtcgaacgt cacgcgcagt ttttcgccgc
     5821 gataatcggc atttaaggca aattctttgt tgtcttctct gtaaccgtgg cgcggggtgt
     5881 cgccgtggcg cagtttgccg ttggcacgca cgccgaatgc tttgttttcg ccgaagcgtt
     5941 gccctaagtc gaacgtacct tgggcgcggt tgttgccgaa ccgggccaaa ccgattttgc
     6001 ggttgccttc gtcggcggct tttttggttt cgatgttgac ggaaccggat accgcgcctt
     6061 cagggtatgc cgtttacggc ggtggacgcg cctttaatca gttgtgcgaa gccgacttgc
     6121 acgctcgccg tgccttgcgt gccatacatt cctgccaagc cgtttacgct gaattggcgc
     6181 gcatcaagct gataaccacg gaaatacaga ccggtcagcg tgttgctttc gccgccaaac
     6241 tctcaaacgg aagcgtcttt tttggctacg gcatccacta aagtacgcgc ctcggtattg
     6301 ttgaaggctt gttcgtcgta gttgacgacg gtaatcggcg cggtaaacgc gttggcttta
     6361 cccaatacgc ccaaatttac gcggtcgcgc aagtcgccat cgctggcgat ggagtaagaa
     6421 tgcgcggctt tgacgcgctt ggcatcggcg cggacatgaa cctgtccgag ttcgccatgc
     6481 tgcggagtat tgtcggcggc agatgaaaac gcgctcaaaa tcaacagcgg cattaaggca
     6541 agttttttgt tcatgaaaac gctctctttt ttaagtattg gggaattaaa gataaaacaa
     6601 acaaacttat cggatatttc gggcggcaat tatatttgat aatcacaact attaccgaaa
     6661 aacttaatca ggcggagcta tccgactgtt aaatattggt ttatttttgt ggctgtacta
     6721 gattatccct aaattccaca ccgatcccgc aggattttta gctgccggga cggtgtgccg
     6781 aagttaagtc gaaattcgca ttccctcaag aacggcgg
//
LOCUS       DAXFZR010000097         6737 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000097 DAXFZR010000000
VERSION     DAXFZR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.098"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            131..577
                     /gene="yciA"
                     /locus_tag="ACFJAS_001899"
     CDS             131..577
                     /gene="yciA"
                     /locus_tag="ACFJAS_001899"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC3983067.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGRIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(628..1449)
                     /locus_tag="ACFJAS_001900"
     CDS             complement(628..1449)
                     /locus_tag="ACFJAS_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC3983068.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1568..2026
                     /locus_tag="ACFJAS_001901"
     CDS             1568..2026
                     /locus_tag="ACFJAS_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC3983069.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2169..3305)
                     /gene="lst"
                     /locus_tag="ACFJAS_001902"
     CDS             complement(2169..3305)
                     /gene="lst"
                     /locus_tag="ACFJAS_001902"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC3983070.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISQKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            3442..3582
                     /locus_tag="ACFJAS_001903"
     CDS             3442..3582
                     /locus_tag="ACFJAS_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HFC3983071.1"
                     /translation="MPPHIRHNAADAAAKRGKAISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            3649..5874
                     /locus_tag="ACFJAS_001904"
     CDS             3649..5874
                     /locus_tag="ACFJAS_001904"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC3983072.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKVVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQALNA
                     L"
     gene            6171..6473
                     /locus_tag="ACFJAS_001905"
     CDS             6171..6473
                     /locus_tag="ACFJAS_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983073.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RAEACYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
BASE COUNT         1794 a         1997 c         1506 g         1440 t
ORIGIN      
        1 atactccttg cgaatctgtc ccgcgcccgc ccttgtatgt ctcaaatggg ttaaactacg
       61 cacttcccga cttcccgttt cgggcaggag tgcaggtccg atttatattt caataaaaca
      121 aggaaacttt atgcagcacg aagaaggcaa ccgccaacgc cctcaaggcg aactgctcct
      181 gcgtaccgtc gctatgccgc gcgataccaa tcccaaccaa gacattttcg gcggccggat
      241 tatgtcgcaa atggatttgg gcggcggcat attggcggcg gaaatcgcgc gggggcgcat
      301 cgttaccgtc gccgttcagg aaatgaactt catccgcccg gtcaaggtcg gcaacgtcgt
      361 ctgctgctac gggcattgcg tccgcgtggg caatacttcc ctccagctta aagtcgaagt
      421 ctgggtgaaa actttgatga acgattgcgt taccgaagac cgccatctcg taaccgaggc
      481 ggtgttcact tatgtcgcca tcgatgcgga aggcaatccg cgcccgattc cgaaagaagg
      541 caaccccaaa ttgtcgggct tattgcccac cccgtaaaaa tacccgtaaa aatgccgtct
      601 gaaactgtct tcagacggca ttccgtatca tttgaaccaa gacctgatgc gggaaaacag
      661 cgacatactc tgtttttcaa agtcttcccg cacttcgggc acggctgttt ttatgacctt
      721 cattcctgca aaagtctgcg ccacaggcat aatttcaatc gtgttcacat tcatatgcgc
      781 cggccggcgg tacagccaca atgcggtttc cgcaatatct tcggggcgga taaattccac
      841 accctcatac acgcccgcca ccctctcgtt atcgcctttg aagcgcacgt tggaaaactc
      901 cgtattgccg cacaaacccg gctcgatatt ggtaacgcgg atgttcttat ccgccagctc
      961 tgcgcgcaaa ttcaggctaa actggcgcac aaacgccttg gtcgccccgt aaacgttgct
     1021 gccggcataa gcataattgc ctgcaatcga acccaaattc atcacataac cgccgccgcg
     1081 ttccaccatt tgcggcaaaa tcttgcgcgt caggaacgtc aaacccaaaa cattggtttg
     1141 aatcattgtt tcccaatctt caaaatccgc cttgtccgcc gtgtccaaac ccaaagccag
     1201 cccggcattg ttgatgaggc agtcgatgtc ggaaaattca tcggggatgc cgttcaaggc
     1261 gttttccacc gactcgcggc acgacacgtc catttccaaa gggtaaaaca aagcacccaa
     1321 ttcatccgcc aaagcctgaa gcctgtccgc acggcgcgcc gcaccgataa cgcggtatcc
     1381 cgccccgaca aacgtgcggc acatcgcttc gccgaagcct gccgaagcac cggtaattaa
     1441 aacagccatt gtttttcctt tctttctcgt ttcccaaacc tgtttcaggt atcataacac
     1501 ccttcagacg gcatcgcgcc ggctgaaaac gacaacaacc gtaccaccac gacaggacac
     1561 attcaacatg aaaacccaaa tcagccttgc cgcaaccgcc ctcgccctat tcctttccgc
     1621 ctgcggaaac ggcggcgcgc ccgcacagcc caaaggcgaa atttccgaaa accgcaccgc
     1681 cgcgttcaaa tctatgatgc ccgacttctc gcgtatgggc aaaatggtca aaggcgaaga
     1741 gccttacgat gtcgaaaaat tcaaacaggc ggcggcggcg tttgccgaaa gcagcaaaaa
     1801 accgttcaca ctttttgagt ccgatccgca aggcaacggc cgcgccctgc ctgccgtttg
     1861 gtcggatggt gcaaaatttg aagccgaaaa aacaaaattc gccgccgccg tcgagaaact
     1921 caacgccgcc gcccaaaccg gcaaactgga cgaaatcaaa gccgcctacg gggaaaccgg
     1981 cgcaagctgc aaatcctgcc acgacagttt ccgcgcaccg gaataacatc cgaaacaaaa
     2041 caatgccgtc tgaagccttc agacggcatt ttctttgtca tttcggcaca cgcaaagcaa
     2101 tcaaaaatta tcgtcaattc aaaaaatacg ccatatcctg atttttgcct tacttactcc
     2161 gccattttct attttgattt accatgcgat tgatttttat cgtcaaatgc caaaatcgga
     2221 atgtcggcct gacggaacag cgcataaacg ggcttgagcc aataatcttc cggaagggaa
     2281 gccggtttca atgcgtaaac gtgcacattg ggaaaatcct tcatcgtcaa cgccgcaccg
     2341 ctgaaaaagg tataaatttc atacctcgta tgcgggtttt tcttaatttc gcgcaaaata
     2401 tagtcttcga tgacataggg cgaatttaac gcggttacgc cggaaagccc gtaggtctgg
     2461 cggggatgcg gcgcgacata ttgtatgttg aagttttttg ccgccttttg cgaaatttcc
     2521 ttcatctcct tgtcgggcga acccaaaagt atccgcaccg tgccgcccgt ttcgtccccc
     2581 gccttcagtt cggacgcatc gaacagcggc aggtaagtca tcttgcggcg gccgtcgtcc
     2641 ataatgtttt tcaaaccctt gaatatcgtg taatgctcgt cggaagcatt gcgggttttg
     2701 gcgatgctcc aatccccgac catcattcgg gcaaaattcc gcttaatcgc cccgtttaca
     2761 taaaactcat cgcccaaata gctgctctcc cgtatcaggt tgtttgtgcc gtcgtcaaag
     2821 gttttgattt ccgcatccgg gtaagtactc aaaaaggcgg caatactgac tttttccaaa
     2881 ctcgccaaat aaatccgcct gaccttcggc agcagcatcg acttcacctt cagctccgcc
     2941 atcgtcggaa tgaaattaaa cgatttgttc aaaccgtagg gcaggtagaa aaaatacgcc
     3001 cgctccgcct tatcctttat ctgattgaaa taataatcgt atttttcatt cctgttttca
     3061 gacatcagca ccacataaaa ccgctccccc ggatgctgcg ccataatcct ttctgccacc
     3121 ttcatctgca atatggtata gcagaaaatc agattgacgg gtttcccctc ttcattgaag
     3181 agtttgtcct tcagcaggga aaccgcgttc ctttccccct gatttacccg gtcaaacgta
     3241 taaaatatcc cgaagcaaaa aacaatcagg cacaacacgg tcaaacagac ttttttcaac
     3301 cccatatccc taaaaactcc attccgacaa attgaacata cgccccgagt gtcccggtgc
     3361 ggaaactccg gtgcggaaaa tgcggaattt tagcagatat tccgacagtt gccgaatatt
     3421 aaaattaatt ttaccgaaaa tgtgccgccg cacatcaggc acaacgccgc cgacgccgcc
     3481 gccaaacgcg ggaaagccat ttcccccttc ctttcccgcc gccgcgttca aacacgccaa
     3541 gatcgaacaa agcgcgaaca aagccgccaa aatatgccat aatagccgcg cacattcccc
     3601 gcgcaatgtg tttcaaaaga aaaaaccaac ctacaagaga ataatcctat gactcaaaaa
     3661 tccaccattg tttataccca taccgacgaa gcccccgcgc tggcgaccca atcgctgctg
     3721 ccgattgtgc aggcgtttgc ccgccacgcc gatattgatg tcaaaactgc cgacatttcc
     3781 ctgtccggcc gcattttggc ggcattcccc gaatatctga ccgaagcgca gcgcgtaccc
     3841 gacgcgcttg ccgaattggg cgaactggtg aaacaacccg gtgcaaacgt aatcaaactg
     3901 ccgaacatca gcgcatccgt acctcaactg accgccgcga ttaaagaatt gcagtctaaa
     3961 ggctttgccg ttcccgacta tcctgccgac cctcaaaccg atgaagaaaa agccgtacgc
     4021 gaacgctacg accgcatcaa aggcagcgcg gtaaaccctg tcctgcgcga aggcaactcc
     4081 gaccgccgcg cgcctaaagt ggtgaaaaac tttgccaaaa aaaatccgca cagcatgggc
     4141 gcgtgggcga aagactccaa aacccacgtt gccaccatgc aaagcggcga ctttttccat
     4201 aacgaacaat ccgttaccgt acccgatgcg acttccgtat ccatcgtgtt caccgacaaa
     4261 caaggcaata aaaaagagct gcgcgagcct gttgccctga aagccggcga aatcattgac
     4321 gcggccgtaa tgagcaaaaa agccctgctc gccttccttg ccgaacaagt gaaagacgca
     4381 aaagctaaag gcgtattgtt ctcgctgcat atgaaagcca cgatgatgaa agtgtccgac
     4441 ccgattatct tcggacacgc cgtcaaagtg ttcttcgcgc ctgtatttga aaaattcggc
     4501 ggcaaactgg ctgccgcagg cgtcaacgtc aacaacggct tcggcaacct gattgccaat
     4561 ctggacaagc tggatgcgga cacccgcgcc gccgtcgaag ctgaaatcgc cgccgtttac
     4621 gccgccaacc ctgatttggc gatggtcgat tccgacaaag gcatcaccaa cctgcacgtt
     4681 cccagcgatg tcatcgttga tgcttctatg cctgcgatga tccgcaattc cggccgtatg
     4741 tgggacaaaa acggcaaagc gcaagacacc aaagccgtga ttcccgacag cagctatgcc
     4801 ggtgtttacc aagcaaccat cgatttctgc cgcgaacacg gcgcattcga tccgacaaca
     4861 atgggtactg tgcccaacgt cggactgatg gcgcaagcgg cggaagaata cggctcacac
     4921 aacaaaacct ttgaaatcga agccgacggt caggttcaag tgattgatgc ggcgggcaat
     4981 gtcctgatgc agcacgacgt tgaggcaggc ggcatctggc gcatgtgcca aaccaaagac
     5041 gctcccgtca aagactgggt acaactcgcc gtcaaccgcg cccgcctgag caacacgccc
     5101 gccgtgttct ggctcgacga aaaccgtccg cacgacaaga gtctgctcgc caaagtcaaa
     5161 gcctaccttg ccgaattgga taccaacggc ctcgacatcc gcgtcctcgc gcccgaagaa
     5221 gccgctaaat tcagcttggg ccgtctgaaa aacggcgaag acaccatttc cgtaaccggc
     5281 aacgtcctgc gcgattatct gaccgacttg ttcccgattt tggaactggg taccagcgcg
     5341 aaaatgctgt ccatcgttcc attgatgaac ggcggcggta tgtttgaaac cggcgcgggc
     5401 ggttctgcac cgaaacacgt ccaacagttc ctcgaagaaa accacctgcg ttgggactca
     5461 ttgggcgaat tcctcgcgct tgccgtatcg tttgaacatc tggcgcaaaa aaccggcaac
     5521 gccaaagccc aagtcctcgc cgacactttg gatgccgcca ccgaaaaact gctgttgaac
     5581 gacaaatcgc ccaaacgcaa agcgggcgaa ctcgacaacc gcggcagcca tttctacctc
     5641 accctctact gggcgcaaga attggcggcg caagacaaag atgccgaact gaaagccgca
     5701 tttgctccat tggcagccgc tttgactgcc gacgaagcca aaatcgttga agaactctct
     5761 gccgtacaag gcaaagcggt cgacatcggc ggctactacg cagccaatcc tgaaaaagcc
     5821 gcacaagcga tgcgtccgag cgcaaccttt aatcaggcac taaacgcctt ataagcacaa
     5881 aggcaaaaat gccgtctgaa cattcgtcgt tcagacggca ttagttatcc ctatctgcct
     5941 gattatgatt aaacgccgca cccattggat atatagtgga ttaacaaaaa ccggtacggc
     6001 gttgccccgc cttagctcaa agagaacgat tctctaaggt gctgaagcac caagcgaacc
     6061 ggttccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
     6121 actataaatc atataatctt tagtttttta ccctacggaa agacaataat atgaaaactg
     6181 ttccaaccat cctgaccggc atccttttgg caaccgccct tcccgcatcc gcacacggta
     6241 tgcataagag caaacccctg gctatggacg agctgccgcc gatttgccaa caatatttca
     6301 aacgcgccga agcctgttac aacaaagccg gaaacaaagc agatttcgca cgcaacaaca
     6361 ccaaattcct attccaagcc ctgcctgccg ccgatttggg acagcgtaag caaatgtgcc
     6421 aaatcgctat ggattcgttt gcagaaaaaa cccgcaatct gaattgcgaa taaagccgca
     6481 tcagcaatag caatgccgtc tgaaacaaag gttcagacgg cattctcaca tcaaaacgca
     6541 ccggctgata aaacccgcct tccccgcatc cctgacccga acaggattaa acggagtaac
     6601 ggtttcggcc attatggcaa gtccccatcc caaaaatgac catataaatt aataccataa
     6661 aacattgata aaaagcgtct gccggttgcg gtttgttgca gggcgttacg gaaaatcatc
     6721 gtaaaccgat gattttt
//
LOCUS       DAXFZR010000098         6657 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000098 DAXFZR010000000
VERSION     DAXFZR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.099"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            149..1368
                     /locus_tag="ACFJAS_001906"
                     /pseudo
     CDS             149..1368
                     /locus_tag="ACFJAS_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(1428..2234)
                     /locus_tag="ACFJAS_001907"
     CDS             complement(1428..2234)
                     /locus_tag="ACFJAS_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC3983074.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            2426..2635
                     /locus_tag="ACFJAS_001908"
     CDS             2426..2635
                     /locus_tag="ACFJAS_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC3983075.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            2724..3938
                     /locus_tag="ACFJAS_001909"
     CDS             2724..3938
                     /locus_tag="ACFJAS_001909"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC3983076.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(4010..6583)
                     /gene="clpB"
                     /locus_tag="ACFJAS_001910"
     CDS             complement(4010..6583)
                     /gene="clpB"
                     /locus_tag="ACFJAS_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC3983077.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVATAELSDRYITDRFLPDKAID
                     LIDEAASRIKMELDSKPEQMDKLDRRIIQLKMEKMHVAKESDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAASSSTADIKKQMDDIKVKIEQAKRQGDFARASELEYGELPK
                     LGAQLQAAESNPDGKKQNKLFRTEVGADEVAEIVSRMTGIPVSKMMEGERDKLLKMEE
                     VLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAGFL
                     FDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDEVE
                     KAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAVKE
                     VVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTVSD
                     AALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRLKF
                     A"
BASE COUNT         1472 a         1652 c         1810 g         1723 t
ORIGIN      
        1 tgctgtgcgg cttgaaaaat aaaatcatat atagattaat aaaatcgggc ttatgtcgac
       61 tacatgctat tacgttttgt cgggaaattg gttatagtaa tgagcgaaat gcacgcccct
      121 gaaaatcttt ttgcgaaaag gaagcaaaat gtctgatgcc gttgcaaaag aaaccctcaa
      181 tccgtttgaa atcgcgcgaa aacaggtcaa aaccgcctgc gaccggctga aaaccgatcc
      241 ggccgtttat gaaatcctga agagtccgac tcgcgtgttg gaagtcaaac tcgacgacgg
      301 cacggtcaaa acctttaccg gttaccgttc gcgtgcgttc ccatcccggt gtgaatctgg
      361 acgaagtcaa ggcactgtcg atttggatga ccatcaaatg ctgcgttgca ggcattcctt
      421 acggcggcgg caaaggcggc gttaccttgg atccgcgcga ttattccgag gcggaactgg
      481 aacgcatcgc ccgagcttat gccgaagcga ttgccccgct gatcggcgaa aaaaatcgat
      541 attcccgccc ccgatgtgaa caccaacggc aaaattatgt cgtggatggt ggatgcctat
      601 gaaaacgtgg tgaaacattc cgcgccgggc gtatttaccg gcaaaccggt tgagttcggc
      661 ggctctttgg cgcgtaccga ggctaccggt tacggcgtga acctcgccgc cgtccaagct
      721 ttggaaaaac tgggcaaaga tgtgaaaggc gcaacctacg ccattcaagg tttcggcaat
      781 gtgggctacc acaccggcta ttacgcgcat caatccggcg cgaaagtcgt taccgtatct
      841 actgttgacg ttgctatcta caatgaaaac ggcttggata tggaagcgct gtttaaagag
      901 ttccaagaaa aaggcttcat caccaacaaa gccggttacg gcaaagaaat taccaacgcc
      961 gaacttttgg ctttggatat ggacgtactc gccccttgcg cattggaaaa ccagctgact
     1021 tccgaaaacg ccggtaaagt ccgcgcgaaa atcgtggttg aaggcgcaaa cggcccgact
     1081 acgcccgaag ccgacgttat cctgcgtcaa aacggcgtat tggtcgtgcc cgatattttg
     1141 gcaaactgcg gcggcgtggt cgtttcctat ttcgagtggg tgcaaaacct gtaaggctat
     1201 tattgggagt ttgacgaagt tcaggaaaaa gaaatcgtcg tcctgcgccg cgcgttccgc
     1261 gatatttgga acttggcgca agagtatgat gtcgatttgc gtaccgcgtc ttatatgatg
     1321 agtatccgcc gtgttgaaaa agcgatgaag ctgcgcggct ggtattgatg tttgaacaaa
     1381 ggatgccgtc tgaaaccatt tcagacggca tttttgtttg gacggaatta atggtgagaa
     1441 tggccgattt ggaaggttaa agttgtgtag ttcgcctgtt tttggcacag gctttgatcg
     1501 gggaaatcgg ctttgtattc gacactcgct ttccaaaagc cttggcgcaa ggggatgatg
     1561 tccacttcgc ctttgccgtc ggtggtgtcg gagaaggctt gggcttcggt tttgtgcgtt
     1621 ttgctgcggt cgctggtgtc gaagccgtca aatgtagcgg taacggtggc attgggcagc
     1681 ggttcgccac ggaacagaac gcggactttg aagcgttcgc ctacgtgaat gttggcggga
     1741 ttgtccagcg ggacgatttc caagttttgt ccgaccggtt tggtgatgat ggcggtgtcc
     1801 gcgctttcgt gtcccacgtt gacaatgttt ttaccgaaca tacgggtttg ttcgcaatag
     1861 cttgcgtcag gcatttcttt gatgccagcc tgtttccagc ctgctttgtt ttttgaccgg
     1921 aaagtaggct gatattcggc ggtaacgagg tagctgccgt ctttgacggg acggttgctg
     1981 cggtattggt agttgtatgt gccgcgttga atcatgtttt ccttaccttt ttcggtaacc
     2041 agctgcatcg gtttgctgaa gatgtgcagg cggtctttgg cgatgggttc gagttcgggg
     2101 aattcgccgt agcccaagtc ggctttaagg tattcgccgc cgtgcgtgtg ggcggtttcg
     2161 acccagacgc ggtgggcgtg tgcggatgtg gcaaatagtg cggaaacggc gagcagtgtc
     2221 aatgttttct tcatggtgca gcctttatat cgtgcgggtt aaaaattttg ttatgctata
     2281 acaaaatatg tatcgtgtga agacggattt gccgcctgaa aactgtcttt ttgtatcgat
     2341 ttgaattttt cagaaaatga agcggacgtt tgggcggagg caacttgttt gataagatag
     2401 caatatttca aaacggagaa atatcatgcc ttatgtcaat attaaagtaa ccggcggcaa
     2461 ggaagcaccg actgccgcgc aaaaagcgga actgatcggc ggcgtaatcg aattgctggc
     2521 acgcgtgctg ggtaaaaatc ccgaaacaac ggttgtcgtg attgacgaag tggataccga
     2581 taactgggat ataggcggca aaagcgtcag cgaacggcgc aaagagggca ggtaaaaagc
     2641 ctgaaaaccc cgccctgatg ctttaaattc cgcgtgaaaa agagtacatt cccacccatt
     2701 gcccaaaatt tacggaacac attatggata aatttcccaa gtctgcaaag ctcgaccacg
     2761 tctgctacga catacgcggg ccggttcaca aaaaagccct tcagttggaa gaggagggca
     2821 acaaaatcct caagctcaat atcggcaacc ctgcgccgtt cggctttgaa gcacctgatg
     2881 aaatcttagt cgatgtcatc cgcaacctgc cgacttcgca aggctattgc gattcgaagg
     2941 ggttgtattc cgcacgcaaa gccattgttc actactatca gaacaaaggt ttgcgcgata
     3001 ttacggtaaa cgatgtctat atcggcaacg gcgtgtccga gctgattgcg atgtctatgc
     3061 aggcgttgct caacgacggc gacgaaatcc tgattcccgc gcccgactac cctttgtgga
     3121 cggcggcggc aacgcttgcg ggcggtacgg tacgccatta tctgtgcgac gaagaaaacg
     3181 gctggtttcc caaccttgcc gatatggaag ccaaaatcac gtccaaaacc aaagccatcg
     3241 tcgtcatcaa ccccaataac ccgacaggag cggtgtacag caaggaaatc ctgctggaaa
     3301 ttgccgaact ggcgcgcaag catggtttga ttattttcgc cgacgagatt tacgacaaaa
     3361 tcctttatga tggcgcggtt cactgccaca tcgccgcgct cgcccccgac cttttgacgg
     3421 taacgttcaa cggtttgtcc aaagcctacc gtgtagccgg gttccgccaa ggctggatgg
     3481 tgctaaacgg gccgaaacat cacgcaaaag gttatatcga gggtttggat atgctctcgt
     3541 ccatgcgctt gtgtgccaat acgccgatgc agcacgcgat tcagacggca ttgggcggtt
     3601 atcagagcat taacgaattc atcttgcccg gcggacggct tttggaacag cgcaacagag
     3661 cgtgggaact ggtcaaccag attcccggcg tgtcctgcgt caaaccgatg ggcgcgatgt
     3721 atatgttccc gaaaatcgat accgaaatgt accgcatccg cgatgatatg aaattcgttt
     3781 acgatttgct ggtgcgcgaa aaagtcctgc tggtacaggg aacggggttt aattggatca
     3841 aacccgacca tttccgcatt gttacgctgc cttacgtcca tcagattgaa gaggcgatgg
     3901 gcaggttggc aagattcctg caaacctacc atcagtagga ttctgataaa aaatgccgcc
     3961 tggaacggag attcccgttt cagacggcat tttcaacagc aggaacaaat caggcaaatt
     4021 tcagtctgtc gccgtcggct tctaccttga tttcgctttc gggcgcatag tttccggcaa
     4081 gcagggcttt ggccagcggg ttttcgattt ccgattggat cgcgcgtttg agcggacgtg
     4141 cgccgtaaat cgggtcgaaa ccggctttgg cgatgatgtc cagagcggca tcggaaacag
     4201 tcaggcgcag gttttgtttt tccaaacgtt tttccaaacc tttgagctgg attttcgcaa
     4261 tactgcggat attggcctga tccaagccat ggaacacgac cacttcgtcg atgcggttga
     4321 tcatttccgg acggaaatgt tctttcacat cctccatcac aacttctttc accgcttcgt
     4381 agtcctgcgt gcccatttgt tggatgtgct ggctgccgat gttggaagtc atcacaatca
     4441 cggtattttt aaaatctacc gtcctgcctt gtccgtcggt caaacggccg tcatccaata
     4501 cttgcagcag gatgttgaat acgtcgggat gggctttttc cacttcgtcc aaaagaatca
     4561 cgctgtacgg tttgcggcgg acttgttcgg tcaggtagcc gccctcttcg tatccgacat
     4621 aacccggagg cgcgccgatt aagcgggcaa cggcgtgttt ttccatgtat tcggacatgt
     4681 cgatgcggat gaggtggtct tcgctgtcga acaggaagcc tgccaaagct ttgcacaact
     4741 cggtcttacc cacaccggtc gggcctaaga acaggaagct gccgtaaggt ttgttcggat
     4801 cggcaaggcc ggagcggctg cggcggatgg catcggacac ggcgcgcacg gcttcgtttt
     4861 gaccgactac gcggcggtgc aacacttctt ccattttcaa caatttatcg cgttcgcctt
     4921 ccatcatttt ggatacggga atgccggtca tacgggaaac gatttcggcc acttcatccg
     4981 cgccgacttc ggtacggaaa agtttgtttt gttttttgcc gtcggggttg ctttccgctg
     5041 cctgcaactg tgcgcccaat ttcggcaact cgccgtattc gagttcggac gcgcgggcga
     5101 agtcgccttg acgtttggct tgttcgattt tgactttgat gtcgtccatt tgtttcttga
     5161 tgtcggcagt gctggaagac gcggcttttt cggctttcca gatttcgtcc aaatcggcgt
     5221 attctttttg caggccgtcg atttcttcgt ctatcagttc caaacgtttt ttgctggcat
     5281 catcgctttc tttggcaacg tgcatttttt ccattttgag ctggatgatg cggcggtcga
     5341 gtttgtccat ctgttcgggt ttgctgtcca gctccatttt gatgcggctg gcggcttcgt
     5401 caatcaaatc aatcgctttg tcgggcaaaa agcggtcggt aatgtagcgg tcgctcaact
     5461 ccgctgtggc aacaatggcg gggtcggtaa tatcgatgcc gtggtggatt tcatagcgtt
     5521 cctgcaaacc gcgcaggatg gcgatggtgt cttccacgct gggttcgccg accaatactt
     5581 tttggaaacg gcgttcgagt gccgcgtctt tttcgatgta ttggcggtat tcgtccaaag
     5641 tagtcgcacc gatacagtgc agttcgcccc gcgccaaggc cggtttcaac atattgcccg
     5701 cgtccatcgc gccgtcggtt ttgcccgcgc cgaccaaagt atggatttca tcgatgaaaa
     5761 tcagggtgtt gccgtcgtct ttcgccaaat cgttcaatac gcctttcaag cgttcttcaa
     5821 attcgccacg gtatttcgca ccggcaatca aagccgccaa atccaaaacc agcaggcgtt
     5881 tgttacgcag ggattcaggt acttcgccgt tgacgatgcg ttgcgccaaa ccttcgacga
     5941 tggcggtttt acccacaccg ggttcgccaa tcagcacggg gttgtttttg gtgcggcgtt
     6001 gcagcacctg aatcgcgcgg cggatttcat catcacgacc gataacgggg tcgagcttac
     6061 cgtctcgggc gcgttgggtc agatcgagcg tgtatttttt caaagcatcg cgttggtctt
     6121 cggcattcgc atcgttcacg ttttgtccgc ctcgtaccgc atcgatcgcg gcattgatgt
     6181 tttgttcggt cgcaccggct tctttcaaga ttttgccggc cgcatcgttc tgctgcacca
     6241 aggcgagcag gaaaagttcg ctggcgatat aggcatcgcc gcgtttggtt gccgctttgt
     6301 ccatcaggtt caacaccgcc tgcaattcgc ggctgggcag aatctcacca ccttgtccgg
     6361 acactttcgg caggctgttc aaatgctgct gcaaacgctg tttaacctgc ggcacgttca
     6421 cgcccgcatg agccaagagc gcggcagctc cgctgttttg gtcgtcaagc agggctttca
     6481 gtacgaaacc tgcttccaga tagctgctgt ccgcagccaa cgccaaactc tgagcttctg
     6541 caagggcttg ttggaatttg gcggttaatt tgtcgtatcg cattttcgtt tccttttcaa
     6601 aatgtccgct gtcgaagcct atatgtgcat gactgtggat aactcaagtg ttgtttt
//
LOCUS       DAXFZR010000099         6377 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000099 DAXFZR010000000
VERSION     DAXFZR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.100"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            14..1234
                     /locus_tag="ACFJAS_001911"
     CDS             14..1234
                     /locus_tag="ACFJAS_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC3983078.1"
                     /translation="MSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAEEIGKHGNWI
                     PFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQLQELLPQTAG
                     NIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAPEASQKVVHL
                     TALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSARPLHDPSLS
                     TSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQYYHPQRNQG
                     TLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQSHFLLNLGI
                     TELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWAGFRFGDICH
                     KLQPSCRLNPLQTA"
     gene            1330..1406
                     /locus_tag="ACFJAS_001912"
     tRNA            1330..1406
                     /locus_tag="ACFJAS_001912"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1364..1366,aa:Met,seq:cat)
     gene            1414..1489
                     /locus_tag="ACFJAS_001913"
     tRNA            1414..1489
                     /locus_tag="ACFJAS_001913"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1447..1449,aa:Ala,seq:ggc)
     gene            complement(1675..2496)
                     /locus_tag="ACFJAS_001914"
     CDS             complement(1675..2496)
                     /locus_tag="ACFJAS_001914"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC3983079.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(2529..3173)
                     /locus_tag="ACFJAS_001915"
     CDS             complement(2529..3173)
                     /locus_tag="ACFJAS_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC3983080.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDGTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(3176..4039)
                     /gene="ftsN"
                     /locus_tag="ACFJAS_001916"
     CDS             complement(3176..4039)
                     /gene="ftsN"
                     /locus_tag="ACFJAS_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC3983081.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(4145..5641)
                     /locus_tag="ACFJAS_001917"
     CDS             complement(4145..5641)
                     /locus_tag="ACFJAS_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC3983082.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHSARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHGAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(5655..5975)
                     /locus_tag="ACFJAS_001918"
     CDS             complement(5655..5975)
                     /locus_tag="ACFJAS_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC3983083.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
BASE COUNT         1544 a         1753 c         1486 g         1594 t
ORIGIN      
        1 accaacgtgt aatatgtcgg gaaatccaat aaatttacac aagctaacat ttatcatgct
       61 cctcccctcc cccgaagcac ggcaatcctc gctcaatctg caaaccctca ttgccgaaga
      121 aatcggcaaa cacggcaatt ggattccatt ttcacgtttt atggaattgg ttttatacgc
      181 tccgcaatac ggctactaca ccggcggcag ccataaaatc ggcaataccg gggattttat
      241 taccgcaccg accctcaccc ctctgtttgc acagacactg gcacgccaac ttcaagaact
      301 cctgccccaa acggcgggca atatctatga attcggcgcg ggaaccggac agcttgccgc
      361 cgatttgttg ggcagcgttt cagacagcat caactgttac tatattattg aaatatcgcc
      421 ggagctggca gcacgtcaga aaaacctgat tcaagcccgt gcaccggaag catctcaaaa
      481 agttgtccac ttgaccgcac ttcccgaagc gtttgacggc atcatcatcg gcaacgaatt
      541 actcgatgcc atacctgtcg aaatcgtccg taaaaatgaa ggaggattac tcgagcacat
      601 cggcgtttgc acggataacg gccgtttcgc ctattctgca agacccttgc acgacccgtc
      661 gctttccacc tcggcttccc tctattttcc tcaaacagat tatccctata ccagcgaact
      721 gcacccgcaa caatatgcct ttatccgcac ccttgcctca aggcttgaac gcgggggtat
      781 gatattcatc gactacggtt ttgatgcggc gcagtattac cacccgcaac gcaatcaagg
      841 cacattgatc ggacactacc gacaccacgt tatccacaat cctttcgact tcatcgggtt
      901 ggcagatctg accgcccacg tcaattttac cgacattgcc caagccggta cggatgcagg
      961 actggatttg acaggctatc tgccccagtc ccattttctg ctgaatctgg gcataaccga
     1021 actgttggca cagacgggta aaacagattc ggcagcctat atccgtgaag ccgccgctgt
     1081 tcaaaaactg attgaccagc atgaaatggg cgaactgttt aaagtcatcg cattcggaaa
     1141 aaatatcggc atcgactggg caggattccg cttcggcgac atctgccaca aactccaacc
     1201 ctcgtgccgt ctgaatccgc ttcagacggc ataaactttt taacatttaa aaacaagaaa
     1261 ctaattcaaa attaaaaaat acggcttgtc aaaaaaacaa aaaaacatat aatagctaat
     1321 tcacgaaacg gcgaattagc tcagtcggtt agagcagagg aatcataatc cttgtgtccg
     1381 gggttcgagt ccctgattcg ctaccagatt ttcgggggta tagctcagtt ggtagagcgc
     1441 ttgcatggca tgcaagaggt cagcggttcg atcccgctta cctccaccag ataaaaaagc
     1501 acagaccgta aaaggtatgt gcttttttat tgcctgattg ccggaaaata aagaataaac
     1561 cactgccttc aaaacaggca atcgacttta aacctattgc cccgcctgtc ctgattttat
     1621 cagcccgcca gacaaacccg acctgaataa tgtcttcagg tcgggtttat ggtttcattc
     1681 ccaacttatc cagcccgaca accacaatat tatgatgaca atgccgaaaa caatgcggta
     1741 ataggcaaac gggatatagt ttttcttgga aacaaacttc agcagtgctt taaccgctac
     1801 caaaccggaa acaaaagcgg caataaagcc tatcaaaatc aaaccgacat catgcagggt
     1861 gaaaaatcgg taatgtttca ggacatcata agccgttgct gcaaccatca tcggaacggc
     1921 caagaaaaat gaaaactccg ttgccgtttt ccgctcgatt ccccaaagca tcccgcccat
     1981 aaccgtactg cccgaacggg acgtacccgg aaccagtgca aacacttggg caacaccgat
     2041 catcaacgca tcaatcggac gcaatgcatc aacatcggca attttaggct ctgctcggct
     2101 ttggcgtttc tccacccaca aaataaaaaa accgcccaaa accagcatga ctgcaacact
     2161 caaggggtta aacagatact ctttgatttg tttgtcgaac aacagcccca tcacggcggc
     2221 aggtataaaa gcaatggcaa gattgaggac gaaacggttg gctttccggt cttttcccac
     2281 gccatgcaac acattgctga aacgctgccg gtattcaaac actaccgcca aaaccgcacc
     2341 gagctggatg gcaatttcaa aaaccttgtg attgctgtga aaaccaatca gattgccgaa
     2401 cacaatcaaa tgtccggtgc tggaaatcgg taaaaattcg gtaaaacctt ctaccaagcc
     2461 catcatcagg gctttcagga caatcagaaa atccattgct tgcgcttctt tcggatacgg
     2521 gagttcggct atttctgtac agcaggggtc tgacgcttgc gttcttccct gactttggca
     2581 accaattctt taatcgtatg aacgccgcca tcaaagccat tattgaagat aacgcggtat
     2641 tttccgccga caataacggt cggcgtgccg tcaataccgt attgttccgt cagtttctgc
     2701 atttttaatg cgacggcggc agcttcgggg gaatcatagg cgcgcatcag ttttttgccg
     2761 tcaaaacctt tttgagataa agcccatttt ccggcaacag ccctgttttc caaacggatt
     2821 ttttgttcgt aaactgcttt aaacacagca gagtttgcct gatatttcaa acccgacagc
     2881 ttgaccgcag cagccattct tgccagaccg agcatttcag gccgccagac cacgtgctcc
     2941 gtccgcagat aagtatcaga cggcaatgcc ttgcccagtt tcaataacaa aggatcgaaa
     3001 tgatggcaat gtacgcaaaa atagccgaaa aattccaaaa cctcaatttt tcccggctgt
     3061 tcttgaggaa tgggtttatc caacacaaga tagtcttccc cttccgtcag ggcatatgcc
     3121 tgcgcggaca acactgccga cagcagcagc ggcaacagat gtttaaactt cataattatt
     3181 tgccttcaat cgcacggatc aggctggcaa ccccatgctt tttcaactcg tcctgcattt
     3241 ttttcaccgc atcggcagac atattgccgc tttgcacgcg gtaaagcgtt ttatgtcccg
     3301 cctgatagcc gaccactttg gaagatatgc ccaagattgc cagtttggca cgctgccctt
     3361 ccgcgctccg gcggtcggca tacgcgccca tttgcagata atgcgttgct tccgccttgc
     3421 caaacgtttt cattttctgc acttctttgg cagcggcact gcgcgctttt tcgatgctgc
     3481 cgctgttgag gatttgttcc ggggtcggtt tgggtgcaac tttttccttc gccgcctttt
     3541 tctcttcttt tgaagctttt ttctctgttt ctttagacgg ttttaccgct tgttttttaa
     3601 ccgtttcggc atctttcttc tgcgcttttt ccctgacggt ttgttcacgc tcttccgtca
     3661 gtgctttctt gcgcactgcc tgtccgtccg gctcttcccg ttccggctcg cccgcctttt
     3721 cttcaacctc gtcggctttg tcggcaacgg gctgcttgtc ggcagctttt tccgcatccg
     3781 actgctctgc ctctttcgca acatccggtt cggacaaggc gttttgatcg gccggttcag
     3841 gttggatgtc ttccttaggc tggtttttca gtttcaggat ttccgtttct gcaggctgct
     3901 tcgacggagc cgggattttg aacgcatttt gaccgccctg gttcagataa agcaaaatac
     3961 cggcaataat gaccgttgcc agtatcaaac cgaagaagaa accggacaga ccttttccgg
     4021 attgggaaaa tttgttcata aacatacctt aatgtgtttc agacggcatt aacgccgttt
     4081 gcttacgggc ggatattcta acaatatcgc catatttggg caaaacctgc ttccattccc
     4141 gttcctataa agcacgacgg aagcctatgg ctttcatgac gtggcttctg ccgacttctt
     4201 catcgcccgc caaatccgcc aatgtacgcg ccacgcgcat aatgcggtgg aagctgcggg
     4261 cggaaagaga caatttttcc agcaggctac ccaaggcttc ctgcgcttct ttttggatgc
     4321 gggcttggga atcgagttcg ctgacgctca aagcggcgtt gactttgccc tgccgtgcgt
     4381 attgtttgcc gcgggcggcg atgacgcgtt ccaaaacgga cgcactgctt tcgcccgctt
     4441 cctgctgcat cagttcggcg gcggacaggc tggggacttc gatggtcaaa tcgatgcggt
     4501 cgagcagcgg accggaaatt ttgcttcggt aacgcgcgac actttcgggc gtgcagcggc
     4561 agggtttgac gggatgcccg agataaccgc acggacacgg gttcatggca gcgacaagct
     4621 ggaatttggc gggatagacg gcttggcgcg ccgcgcggga aatgtggatt tcgccgtttt
     4681 ccaacggttc gcgcaaaacc tccaaaactt tgcggtcgaa ctcgggcaat tcgtccaaaa
     4741 ataaaacgcc gtgatgcgcc aatgaaatct cgcccggacg cggatccgag ccgccgccga
     4801 ccatagccgc cgcgctggcg ccgtgatgcg gactgcggaa aggacggttg ctgtcgagtt
     4861 gttgttggtg gttgggcagg agcgaacgca atgcccaaac ttctaccaat tcgtcttcgg
     4921 ccagcggcgg caggatgccg ggcagccgtt gggagagcat agacttgcct gttcccggcg
     4981 ggcccatcat caagaggctg tgtccgcctg cggcggcgat ttccaaagca aggcgggcag
     5041 agtgctgacc tttcacatca cacaaatcag gttgtccgcc atgttcagac ggcatttgcg
     5101 gtacggaaca ttcggtttgc gccaaaggtt cgataccgtt caaatgagcg gcgacttcgc
     5161 ccaaagagcg cgcgccgtaa acggtaatgc cgcgcatcac ggcggcttgt cctgcgtttt
     5221 cttcaggcaa aacaaatgca cgttttgcct gcataccctg ccacgccatc gccaacgcgc
     5281 cgcgcacggg gcgcaacaac cccgacagtg ccaattcccc cgcaaactcg tattccgcca
     5341 gcttttcggg tgcaacctgc cccgatgcgg caaggatgcc gattgcaatc ggcaaatcga
     5401 aacgccccga ctctttgggc aggtcggcag gggcgaggtt gacggtgatt tttttggcgg
     5461 ggaattcaaa accgctttga ataatggcgg cacggacgcg gtcgcggctt tcctttactt
     5521 ccgtatcggg cagtccgacg atgttgaaat gcggcaggcc gttggcaagg tgggcttcca
     5581 cttcgaccaa cggcgcattc ataccgctca aggcgcggct gtaaaccaag gcaagcgaca
     5641 tatttcagac ggccttattc gccggcttcg gtttgctgcc tgatttcggc gacggcttct
     5701 tcggcagcgg cttcagccgc ttccaatgct gcccgttcgg gattttgcgc ggcttcgagt
     5761 ttcgccagac gtgcttccaa agccgccagt ttggtacggg ttttaatcaa aacctgctgc
     5821 tggatgtcga attcttcgcg cgtaaccaaa tccatacggt tgaacgcgcc gcccagcatc
     5881 gccttaatat ttttttccac atctttggca gggctgttgg cgatggtttc gctgattttc
     5941 gagccgactt cttcaaaaag ctgcttgccg aacataatct gcatccttcc tgaacatatc
     6001 aaattcaagc ggctattgta taaggaaaaa tgccgtctga aaacgggcgg cggataatcg
     6061 tcaaaacata ccgcgccccc tttgcggttg ctaaaattac acttcgagac acctgccgcc
     6121 tgaaaacgga tttcatattc cgcgcaacgc ccgattacat tacaagacac tacaaaacaa
     6181 tatgctgttt taaatgattt ttccgatgcg catcgtttca aactcggctt tttaagccat
     6241 taagtgcttt gcaaacaaca gaaattgggt tatgctgaaa cggattattt acaatttcat
     6301 atagtttcat tacatatctt attgtaattg aagataattt atccgaatcc cctttcgggt
     6361 atccggattt tccgttg
//
LOCUS       DAXFZR010000100         6264 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000100 DAXFZR010000000
VERSION     DAXFZR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.101"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            148..1983
                     /gene="edd"
                     /locus_tag="ACFJAS_001919"
     CDS             148..1983
                     /gene="edd"
                     /locus_tag="ACFJAS_001919"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC3983084.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            2192..2830
                     /locus_tag="ACFJAS_001920"
     CDS             2192..2830
                     /locus_tag="ACFJAS_001920"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC3983085.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEI
                     TLRTPVGLEAIRLIAKEMPNAIIGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAG
                     RNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(3019..3204)
                     /locus_tag="ACFJAS_001921"
     CDS             complement(3019..3204)
                     /locus_tag="ACFJAS_001921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983086.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(3215..3327)
                     /locus_tag="ACFJAS_001922"
                     /pseudo
     CDS             complement(3215..3327)
                     /locus_tag="ACFJAS_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3425..4465)
                     /locus_tag="ACFJAS_001923"
     CDS             complement(3425..4465)
                     /locus_tag="ACFJAS_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFC3983087.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSQLEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            4628..5668
                     /gene="mutY"
                     /locus_tag="ACFJAS_001924"
     CDS             4628..5668
                     /gene="mutY"
                     /locus_tag="ACFJAS_001924"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC3983088.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            complement(6044..>6264)
                     /locus_tag="ACFJAS_001925"
     CDS             complement(6044..>6264)
                     /locus_tag="ACFJAS_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983089.1"
                     /translation="CSHFFIGFIIHHLIYGFWRQGLTAFAAVIDFWNRGNAFKESKAI
                     RPDADGGAIIHLLEFVAIGADSCDDYVE"
BASE COUNT         1617 a         1768 c         1491 g         1388 t
ORIGIN      
        1 gacagtacaa atagtacggc aaggcgaggc aacgccggac cggtttaaat ttaatccact
       61 atactcaccg tctaacatac ccgatacaaa aatcagaaac gcacaaacaa atccccaata
      121 ccaccccgtt ccgccaggag accgaacatg aaccacactc ctatccaccc caaactcgcc
      181 gaaatcaccg ggcgcattat cgaacgcagc cgtccgacgc gtgaaaaata tctggcgaaa
      241 atccgcagtg ccaaacagat gggacgctta gagcgcaacc agctcggctg cagcaatttg
      301 gcgcacggct atgccgccat gcccaaaagc atcaaaatcg aaatgcttca ggaaaccgtc
      361 cccaacttag gcatcatcac tgcctacaac gacatggttt ccgcacacca gccgtttaaa
      421 gacttccctg accaaatcaa agacgaagca caaaaaaacg gcgcgaccgc acaagtcgcc
      481 ggcggcacgc ctgccatgtg cgacggcatc acgcaaggct acgccggcat ggaattgtca
      541 ctgttttccc gcgacgtgat tgcgatgagt accgccgtcg ggctgtcgca ccaaatgttt
      601 gacggcagcc tgtttatggg cgtgtgcgac aaaatcgttc cgggtctgat gataggtgcg
      661 ctttcgttcg gtcatattcc cggtatcttc gttccggcag gcccgatgtc cagcggcatc
      721 ggcaataaag aaaaagcccg tacccgccaa cttttcgccg aaggcaaagt cggacgcgac
      781 gaacttttga aaagcgaaat gggttcttac cacagcccgg gcacctgcac tttctacggc
      841 acggcaaact ccaaccaaat gatgatggaa atgatgggcg tacacctgcc tgccgccgcc
      901 tttgtccacc cctataccga cctgcgcgaa gccctgaccc gctacgccgc cggacacctc
      961 gcgcgcggca tcaaaaacgg cacgattaaa cctttgggcg aaatgttgac cgaaaaatcc
     1021 ttcatcaatg ccctgatcgg tttgatggcg accggcggct cgaccaacca caccatgcac
     1081 ctcgtcgcta tggcgcgcgc cgcaggcgtg attttgaact gggacgactt cgacgaaatt
     1141 tcttccatca tcccgctgct catccgcgtg tatcccaacg gcaaagccga cgtgaaccac
     1201 tttaccgcag caggcggcct gcctttcgtt atccgcgaat tgctggacgc aggcttattg
     1261 cacgacgatg tcgataccgt cgtcggacac ggtatgcgcc actacactaa agagcctttc
     1321 ctcatcgacg gcaaactcga atggcgcgaa gcccccgaga ccagcggcaa cgacgacatc
     1381 ctgcgtaaag ccgacaatcc gttctccccg gacggcggtc tgcgcctgat gaaaggcaac
     1441 atcggacgcg gcgtgattaa agtgtcagcc gtacgcgaag gctgccgcat catcgaagca
     1501 cccgccatcg tgttcaacga ccagcgcgaa gtgttggcgg catttgaacg cggcgagttg
     1561 gaacgcgatt ttatctgcgt cgtccgctac caaggcccgc gtgccaacgg tatgcccgaa
     1621 ttgcacaaac tgaccccgcc tttgggcatc ctgcaagacc gcggcttcaa agtggcgctg
     1681 ctgaccgacg gccgtatgtc cggcgcgtca ggcaaagtgc ccgcgtccat ccacatgacg
     1741 cccgaagccc tgatgggcgg caacatcgcc aaaatccgta ccggcgacct gattcgcttc
     1801 gactccgtta ccggcgaact caacgtcctg attaacgagg ccgaatggaa cgtccgagaa
     1861 gtcgagcgca tcgatttggg cgcgaaccaa caaggctgcg gccgcgaact cttcgccggc
     1921 ttccgcagta tgaccagcag tgcggaaacc ggtgcgatga gcttcggcgg agaatttgcc
     1981 tgatgcgcgt ttcagacggc cttttaaaac agaaggccgt ctgaaaaatt attgaagccg
     2041 tttaaaatag atgcagacgt agcgtgagct ttgcccgcga acaaaaccca agcaacatca
     2101 gaatgtttta ccccaaaggt tgtctgaaaa cttaaccccc ccatcccgtt tgacctcaac
     2161 aaaaacgaca ccccccactt ggagaaccga aatgtccaaa ctgacccccc gcgaaatcct
     2221 gaccgccggc gcagttgtgc ctgtgatggc gattgacgac ttaagcaccg ccgtcgattt
     2281 gtcccgcgcc cttgtcgaag gcggcatccc caccctcgaa attaccttgc gcacccccgt
     2341 cggactcgag gccatccgcc tgattgccaa agaaatgccc aacgccatca tcggcgcagg
     2401 tacggtaacc aatcccgaac agctcaaagc cgtcgaagac gcaggcgcgg ttttcgccat
     2461 cagcccgggt ttgcacgaat ccctcgccag agccggccgc aacagcggca ttcccctgat
     2521 tcccggcgtt gccactccgg gcgaagtcca actggctttg gaacacggca tcgacacact
     2581 caaactcttc cctgccgaag tcgttggcgg caaagcgatg ctcaaagccc tctacggacc
     2641 ttacgccgac gtacgcttct gcccgaccgg cggcatcagc ctcgccactg cgcccgatta
     2701 cttggcattg cccaacgtct tatgcgtcgg cggctcttgg ctgacaccga aagaagccgt
     2761 gaaaaacaaa gactgggaca ccattacccg cctcgccaaa gaagcggcgg cattgaaacc
     2821 caaagcctga accgcatcgt aaaaatgccg tctgaagccc tttccagtct cagacggcat
     2881 tttgccgctt ggcgactcag tcggcataca cagcagcacc gcccctaaaa tgcccatcca
     2941 tctccagaac aatgcgaatg acgcgcgata tgcggagacc tatgacgaaa aaataattat
     3001 aatatattta tcaattagtc ataaaaatcg ccctatcggt aaaatgccgt gatgagcggc
     3061 tgaaactaac aagttaaccg tcgttaaaaa ataacgctca gagacctttg caaaattcct
     3121 tttcttcaaa cagccgacat ccaaccacag gtttttcggc tgttttcgtt tcaaataacc
     3181 gccgttccca ccaaaatatc ccattaatcc cccttaaata tagtggatta aatttaaacc
     3241 agtacggcgt tacctcgcct tgccgtacta ttttactgtc tgcggcttcg ttgccttgtc
     3301 ctgatttaaa tttaatccac tatacctgat tgatcaggaa cagcccgttt tacacggctg
     3361 aaaaaatgcc gtctgaaaca cgttcagacg gcatccgctt aaaaaacaac tgattcaacg
     3421 gcgattaatc cgcttccaaa accactttca tcgcttggtt ttcggcggcg tgtttgaaca
     3481 cgtcgtaggc tttttccaat tggctgaatt tgaaacggtg ggtcaacatt ttggtgtaat
     3541 ccactgagct gctgaaaatt gccttcatca gtatttcggt ggtgttggca ttgaccaaac
     3601 cggtagtgat ggcaagattt ttaatccaca gtttttccag tttgaaatca acggattgac
     3661 cgtgtacgcc gacgacggcg atatgaccgc cgggtttcac gatgtcttgg cacatattcc
     3721 aagttgcagg gataccgacc gcttcgatgg cacaatctac gccgtcttcg ccgacgatgg
     3781 cgaaaacttg tttggagacg tcgccggaag cggggctgat ggtatgggtc gcgcccaact
     3841 ctttcgccgg tttcaaacgg ttttcgtcca tatcgcacac gataatggcg gcagggctat
     3901 acagttgggc ggtcaacaag gcggacatac cgacagggcc ggcacctgcg atgaatacgg
     3961 tatcgccggg tttcacatcg ccgtattgca cgccgatttc gtgggcggtc ggtaaagcgt
     4021 cgctcaacag cagggcgatt tcttcgttga cgttgtcgtg cggcggcacg aggctgttgt
     4081 cggcataagg cgtgcggacg tattcggcct gcgtgccgtc aatcatatag cccaaaatcc
     4141 agccgccgtt gcggcagtgc gaataaagtt ggattttgca gttgtcgcaa gtgcagcatt
     4201 tgctgacgca tgaaataatg actttatcgc cgactttgat gttttttaca gcctcgccga
     4261 cttcttctac aataccgatg ccttcatgac cgaggatacg accgtcggcg acttcggggt
     4321 ttttgccttt ccaaataccc aagtcggtac cgcaaatcgt ggttttgacg attttcacca
     4381 ccgcatcggt cggatcgata atctgcgggc ggggtttttc ttcaaaacgg atgtcgtttg
     4441 cgccgtgata aaccattgct ttcatgctga tactccttgc ttgttgataa ataatttcaa
     4501 taccgcaaca aagtttcttt atatgagtta tatgccccta caaaaaataa gtcaataaga
     4561 attattttca caattttata caacaacata ctgttttaaa tataaataaa accaccgatt
     4621 gatattaatg aacacaccca tccccttctc cgaacggctc atccgctggc aaaaacaaca
     4681 cggtcgccac cacctccctt ggcaggtcaa aaacccttat tgcgtctggc tttccgaaat
     4741 catgctccag caaacgcagg tcgccgccgt gttggactac tatccgcgtt tcttggaaaa
     4801 attcccgacc gttcagacgc ttgccgccgc gccgcaagac gaagtgttgt cgttgtgggc
     4861 gggcttgggc tattacggcc gcgcgcgcaa tctgcacaaa gccgcgcaac aaatcgtcgg
     4921 acaattcggc ggtacgtttc catcggagcg caaagacttg gaaacgctct gcggcgtagg
     4981 cagaagcacc gccgccgcca tttctgcctt tgctttcaac cgacgagaaa ccatcttgga
     5041 cggcaacgtc aaacgcgttc tctgccgtgt ttttgcccaa gacggcaatc cgcaagataa
     5101 aaaatttgaa aactcgctct ggacacttgc cgaaagcctg atgccgtctg aaaacgccga
     5161 tatgcctact tacacgcaag gtttgatgga tttgggcgcg accgtgtgca aacggacgaa
     5221 acccttgtgc cgtcaatgcc ctatggcgga catctgcgaa gcaaaaaagc aaaaccgcac
     5281 cgccgagctg ccgcgtaaaa aaactgccct cgaagtgcaa accctgccgc tttactggct
     5341 gattgtccgc aaccgggacg gcgcaatctt gctggaaaaa cgccccgcca aaggcatttg
     5401 gggcggactg tattgcgtgc cgtgttttga aagtttgaac gggctttccg actttgccgc
     5461 caaactctcc ctgacgatgg cggatatgga cgaacaaacc gccctgaccc accgcctgac
     5521 gcaccggctg ctgatgatta cgccgtttga agggcaaatg ccgtccgaac accattcaga
     5581 cggcatttgg ataaagccgg gccatttgaa agattacggc ctgcccaagc ctttggaaat
     5641 ttatttaaac ggtaataggt tagaataaac aaaataaacc cgttgaactg ttgttagcag
     5701 gtatcgcagc aagaacaacc gatgaatttg ggtcgtattt taggcagcgg gataatgttc
     5761 aaatgggaca tttggaacgg aaaaagtcgg caatttaaaa aggattttag aaacaaagag
     5821 ggtcaaaaac atgaacacaa acttaaatga caaagacaaa gccatggata ccgcaatcag
     5881 gtttcagaaa aggatgagga ttccgaaatt tttcttttta attctcggaa tcacagtggt
     5941 tttggcattt atccaagacg tgataacggg ttctaatttt ctgcaaataa caattatgta
     6001 aataatattt gtgatatatt tcagtttcgg aggtatcata attttattct acataatcat
     6061 cgcaggaatc tgcccctatt gccacgaatt ccaaaagatg aattatggca cctccgtcgg
     6121 catcgggtcg gatagcttta ctttctttaa aggcatttcc cctgttccaa aaatcgatta
     6181 ctgcagcaaa tgcggtaagc ccctgtctcc agaagccgta aataaggtga tgaatgataa
     6241 aaccgatgaa gaaatgtgaa cagc
//
LOCUS       DAXFZR010000101         6259 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000101 DAXFZR010000000
VERSION     DAXFZR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.102"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(67..1305)
                     /locus_tag="ACFJAS_001926"
                     /pseudo
     CDS             complement(67..1305)
                     /locus_tag="ACFJAS_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1485..2858)
                     /locus_tag="ACFJAS_001927"
     CDS             complement(1485..2858)
                     /locus_tag="ACFJAS_001927"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC3983090.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3072..4076
                     /gene="dusB"
                     /locus_tag="ACFJAS_001928"
     CDS             3072..4076
                     /gene="dusB"
                     /locus_tag="ACFJAS_001928"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC3983091.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLAS
                     DPTLRNTGKTLHRSDFADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPAIAKIAED
                     CGIAALAVHGRARTQMYKGEARYELIAETKSRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECRAAILNHIRAMHAFYGETVGV
                     RIARKHIGWYIGEMPDGEQARREINRLDNAAAQYDTLAGYLERLAGKTDRWACGYREG
                     "
     gene            4089..4238
                     /locus_tag="ACFJAS_001929"
     CDS             4089..4238
                     /locus_tag="ACFJAS_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983092.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4265..4504
                     /locus_tag="ACFJAS_001930"
     CDS             4265..4504
                     /locus_tag="ACFJAS_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC3983093.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4507..5043
                     /gene="ruvC"
                     /locus_tag="ACFJAS_001931"
     CDS             4507..5043
                     /gene="ruvC"
                     /locus_tag="ACFJAS_001931"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC3983094.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5094..5963
                     /locus_tag="ACFJAS_001932"
     CDS             5094..5963
                     /locus_tag="ACFJAS_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC3983095.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
BASE COUNT         1364 a         1609 c         1854 g         1432 t
ORIGIN      
        1 tgaatgatga aataaaaatg ccgtctgaac ccgattggat tcagacggca ttttgtatgg
       61 caggatttat tcgctttcca actgaccgac ccattctgac aaggcagtca ggcgttgttc
      121 ggatttcgcc acgaacgggt tttcggtatc ccacgcgtag cctgccaaaa tcgaagaaag
      181 catacggctg atttccgggc tgcggtcggg cgcgtacacg acgttttgga agcggaaatc
      241 ataccagccg tccacatagg tgcggaacgc gtctacgccg atcatcaggg gttcggcaaa
      301 ttcggtttgc caatcggcgg cttcgccttt gagttgtttt gtcagcagat cggcagcaag
      361 ttcggcggag tgcagcgcga tggttacgcc cgacgagaac acggggtcga ggaactcggc
      421 ggcattgccc aacagcgcga aatgcctgcc gtgcagtgat ttgacgttgg cggaatagcc
      481 ttggatggag cggaacggaa aatcgttttc ccaaacggct ttgtccaaaa tttcgctcaa
      541 catcgggcat tcgtaaacaa attttttcaa caccgtttcc gattcgccgg caagtttgtc
      601 gggtgtgccg accacgccga cggaacagcg gttgtcgccg aagggaatca gccaaatcca
      661 cacgtcgcgg ttgttgcgga tgggtggtaa tcaggatttt gttgcggtcg aatttcgggt
      721 gggtaatgtt gtcgtcgatg tgcgtgaaat gcgtttggcg cggcggcagg tgcgagggcg
      781 tttccaagtt tagcaggcgc ggcagcacgc gtccgtagcc gcttgcgtcc aagacgaatt
      841 tcgcggtcag ttcatagctc tcgccggtgt cggtttcgat gttcaagcgg gcaaaatcgc
      901 cgctgttgtc gaacgcggtt acgccgtgcc cgaaacgtac ttcaacgcct tgtttggcgg
      961 cttcttcaat caggattttg tcgaacacgg cgcggcggac ttggtaaacc gtgccggggc
     1021 cgtctgaaaa tttatcggtg aaatcaaact cggtatagcg gctgccccac gaaaacgccg
     1081 caccgttttt caactgaaag ccgggcccgg tgcgaacggc atcggcaaaa ccggcttctt
     1141 ccagcatttc catacagtgc ggcagcaggc tttcgccgat gacgaagcgc ggaaagtgct
     1201 gttttccaac acgcagactt gataaccttt tttgcggagc agggcggatg cggccgaacc
     1261 tgccggacct gcgccgatta cggcgacatc gaattgggtg gacattggga aatcctgatg
     1321 gtttgagtgg aaaaggaaat gatattgtac aggcaggaaa tgccgtctga aactgtttgc
     1381 ggaagaaatt tatccggtgc gggcggttgc tcccaatgcg tcagtccggt ttgaaaaatg
     1441 ccgtctgaaa cgggaaatgt tcagacggca tttgattttc agggttattt tacgccgtaa
     1501 cgcggttgga gcagggcgca cgtccggtgt ccccggcggc ttcggcggct gcgcccggca
     1561 atttttccgc aggccgcgcc cgcatcgttt gccgtggttt ccggccgggt atttttttcg
     1621 cgctgagccc tggcggattc gtagcggttg cgttgtctcg gttcgcttgt ttcggttttt
     1681 tccggttttg agccttcctg ttcccaccat cggggctcga agccttcgat gcgttcgatg
     1741 agcagcttgt tgccggtcag ttctttgatg gcctcaaaca ttttctgttc ggattcgtcc
     1801 atcagggaaa tcgccacgcc gtccgcaccg gcgcgccccg tgcgtccgat gcggtggatg
     1861 tagtcttcgg gctgggcggg catttcgtaa ttgatgacga agggcagttc ggcgatgtcc
     1921 agcccacgcg cggcgatatc ggtggcgacg aggacgcgca ggctgccgtc tttgaaggcg
     1981 ttgagtgttt cgagcctgct ttgttgggaa cggtcgccgt gtatcgcctg tgcggacagg
     2041 ttgcggcgca ccagttcgcg cgttacgcgg tcgacgcttt gtttggtttt gcaaaagacg
     2101 ataacttggt tcatatgcag atcgacaatc agccgttcga gcaggttgcg cttttggaag
     2161 gtatcgacgg cgatgatgtg ttgttcgacg ttggcgttgg tggtgttttg ggcggcaacc
     2221 tcgacggttt cgggcgcgtt catgaagtct tgcgccagtt tgcgtatcgg tgcggagaag
     2281 gtggcggaaa agagcagggt ttggcgttgg cggggcagca tctgcatgat tttgcggatg
     2341 tcgtcgataa accccatatc caacatgcgg tctgcttcgt ccagaacgac gatttcggct
     2401 ttgtttaaac tgatgttttt ctgtttcaca tggtcgagca gccgtccgac ggtggcgacg
     2461 actatttcgc agccggcacg caggtcggcg gtttgtttgt ccatgttgac accgccgaag
     2521 aggacggtat gccgcagcgg caggttttta atgtagccct gcacgttttg gtcgatttgg
     2581 tcggcaagtt cgcgcgtcgg ggtcaacacg agcatacgca cggggtgcat tgcaggcgag
     2641 gtgctggcgg tggcgtaacg tttgaggcgt tccagactgg gcagcataaa ggcagcggtt
     2701 ttgcctgtgc cggtttgcgc ggcggcaagc aggtcgtgtc cggccagtgc tttgggaatg
     2761 gccgcggctt ggatgggcgt cgggttttcg taaccttgcg cggtcagtgc ggaaaccagt
     2821 tccgtaccca aacctaaaga ggaaaatgga ttactcatga ttgtagtctt tctttcagac
     2881 cttatgccgt ctgaagcggg aaaccgatag gacggggaaa taaactgccc gcctgtgaac
     2941 ggcggggacg ggaatttgtg tgcggattat gccataaaac ggtgcccgcc ccaatcgcgg
     3001 gcgcgtccgc agattggaaa tcctgcttaa aaaatgtaca atggcgcact tttttgaaac
     3061 gcgggcccat tatgcacatc ggcggttatt ttatcgacaa ccccatcgca cttgcgccga
     3121 tggcgggcat taccgacaaa cccttccgcc gcctctgtcg ggcgtttggc gcaggttggg
     3181 cggtgtgcga aatgctggcc agcgatccga cgctcaggaa taccggaaaa accctgcacc
     3241 gcagtgattt tgccgatgaa ggcggcatcg ttgccgtgca gattgccggc agcgaccccg
     3301 aacagatggc ggatgcggcg cgttacaacg tcggactcgg ggcgcaggtc atcgacatca
     3361 atatgggctg ccccgccaag aaagtgtgca acgtccaagc cggtagcgcg ctgatgcagg
     3421 acgagccgct ggttgccgcc attttggagg cggtggtcaa ggcggcgggc gtacccgtta
     3481 ccctcaaaac ccgtttgggt tggcacgacg accatcaaaa cctgcccgcc atcgccaaaa
     3541 tcgccgaaga ttgcggcatt gccgcccttg ccgtccacgg gcgcgcgcgc acgcaaatgt
     3601 acaaaggcga ggcgcgttac gaactcatcg ccgagaccaa aagccgtctg aacatcccgg
     3661 tctgggtcaa cggcgacatc acttcgccgc aaaaagccgc cgccgtcctc aaacaaaccg
     3721 ccgccgacgg catcatgata gggcgcggcg cgcaaggcag gccgtggttt ttccgcgatt
     3781 tgaagcatta tgccgaacac ggcgttttac cgcctgcctt gagtttggca gaatgcagag
     3841 ccgccatttt gaaccacatc cgcgccatgc acgcgtttta tggtgagacc gtcggtgtgc
     3901 gcatcgcacg caaacacata ggctggtaca tcggcgaaat gcccgacggc gaacaggcgc
     3961 ggcgtgaaat caaccgcttg gacaatgcgg cggcgcaata cgacacactt gccggttatc
     4021 ttgaaaggct tgccggaaaa accgaccgtt gggcgtgcgg ctatcgggaa gggtagggca
     4081 gtattgccat gccgccgttc gggttcggac ggcatctgtc tgcatggttc ggaggtcggg
     4141 cggaatcccg gccggcgaat gtcgaaaaca ataaatgccg tctgaaaacg agtggaacgg
     4201 gtttcgccaa aaggctttca gacggcattc cgtcttaaaa acatttcaat caaaaggata
     4261 tgcgatgaac cctgcgactg cggacattgc gcaatgtatc gagcagaact tgaaccaata
     4321 tttcaaagac ctgaacggca ccgaaccttg cggcgtgtac gatatggtac tgcatcaggt
     4381 ggaaaagccg ctgctggtgt gcgtgatgga gcaatgcggc ggcaaccagt ccaaagcctc
     4441 cgtgatgctg ggactgaacc gcaatacctt gcgtaagaaa ctgattcaac acggtttgct
     4501 gtgaatatgt cggcaaccgt ccgtatcttg ggcatcgacc cgggcagccg cgtaacgggt
     4561 ttcggcatca tcgatgtcag ggggcgcgat catttttacg tcgcctccgg ctgcatcaaa
     4621 acgcctgccg atgagcctct ggcagacagg attgccgtga ttgtgcggca tatcggcgaa
     4681 gtcgttgccg tttacaagcc gcaacaggcg gcggtggaac aggtgttcgt caacgtcaat
     4741 ccggcatcga cgctgatgct cggtcaggcg cgcggagcgg cattggcggc tttggtcagc
     4801 cataagctgc ccgtttcgga atacacggcc ttgcaggtca aacaggcggt ggtcggcaaa
     4861 ggcaaggcgg cgaaagaaca ggtgcagcat atggtggtgc aaatgctggg actttcggga
     4921 acgccgcagg cggatgcggc ggacggtctt gccgtcgcgc tgacccacgc cttacgcaac
     4981 cacgggcttg ccgccaaact caatccttcg gggatgcagg tcaagcgcgg aaggtttcaa
     5041 tagtttcaga cggcatttgt attttgccgc ctgaaaagaa aatgtgtacc gagatgaaat
     5101 ttatattttt tgtactgtat gttttgcagt ttctgccgtt tgcgctgctg cacaagattg
     5161 ccggcctgat cggttcgctt gcctaccttc tggtcaaacc gcgccgccgt atcggcgaaa
     5221 tcaatttggc aaaatgtttt cccgaatggg acgaagaaaa gcgtaaaacc gtgttgaaac
     5281 agcatttcaa acacatggca aaactgatgc tcgaatacgg cttatattgg tacgcgcctg
     5341 ccaaatgcct gaaatcgctg gtgcgctacc gcaataagca ttatttggac gacgcgctgg
     5401 cggcggggga aaaagtcatc atcctgtacc cgcactttac cgcgttcgag atggcggtgt
     5461 acgcgcttaa tcaggatgtc ccgctgatca gtatgtattc ccaccaaaaa aacaagatat
     5521 tggacgaaca gattttgaaa ggccgcaacc gctatcacaa cgtcttcctt atcgggcgca
     5581 ccgaagggct gcgcgccctc gtcaaacagt tccgcaaaag cagtgcgccg ttcctgtatc
     5641 tgcccgatca ggatttcgga cgcaacaatt cggtttttgt ggattttttc ggcattcaga
     5701 cggcaacgat taccggcttg agccgcattg ccgcgcttgc aaatgcaaaa gtgatacccg
     5761 ccattcccgt ccgcgaggcg gacaatacgg ttacattgca attctatccc gcttggaaat
     5821 cctttccgag tgaagacgcg caagccgacg cgcaacgtat gaaccgcttt atcgaagaac
     5881 gcgtgcgcga acatccggaa caatatttct ggctgcacaa gcgtttcaaa acccgtccgg
     5941 aaggcagccc cgatttttac tgactacata aaattacaaa acaaatcagg cgtttcagat
     6001 caaaaacccc gattgttttt gggaatttga aacccgggtt gtacaaacag gatttgccgg
     6061 acggttttaa cggttcagtt gtttgtaaaa acaatgcttt tttaaaattg acaaaaaacg
     6121 aaatcggttt taaaggctta ttccgagaac aaaggggagt ggatgccgaa aacccggtta
     6181 atatattata gtggattaac aaaaaccaat acggcgttgc ttcgccttag ctcaaagaga
     6241 acgattccct aaggtgccc
//
LOCUS       DAXFZR010000102         6221 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000102 DAXFZR010000000
VERSION     DAXFZR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.103"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            452..1372
                     /gene="ribF"
                     /locus_tag="ACFJAS_001933"
     CDS             452..1372
                     /gene="ribF"
                     /locus_tag="ACFJAS_001933"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC3983096.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVAVVFEPQPKEFFALRTGKTPPCRISPLRTKLELLEGTGCVDAAWVLRFDRNF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTTVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDGGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            1514..4303
                     /gene="ileS"
                     /locus_tag="ACFJAS_001934"
     CDS             1514..4303
                     /gene="ileS"
                     /locus_tag="ACFJAS_001934"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC3983097.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDNPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVLKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGGYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(5964..>6221)
                     /locus_tag="ACFJAS_001935"
     CDS             complement(5964..>6221)
                     /locus_tag="ACFJAS_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3983098.1"
                     /translation="KTIEVTTIPHAPNATPTIYRVPKTQDAHQESDSIRRVGLGVIAG
                     VGFDITPNLTLDAGYRYHNWGRLENTRFKTHEASLGMRYRF"
BASE COUNT         1440 a         1675 c         1751 g         1355 t
ORIGIN      
        1 gagaagagaa gagaagagaa gttttttgcg ggctggattc attttcggct ccttatatcg
       61 gtttgaccgg ttaaaaaaaa gattttcact gatgcttcaa aggcggatta tatcgggttc
      121 cgggcggtgt ttcaatacat agcaccgcgc ctgctgcgcg ttttatgcgt ttggcgcgtt
      181 cggcggcggg aaatttgcct acttttcccg cgtcgggcgg gcggaacggg cggcacactg
      241 tctataaacc gcaataccgt ttacaatgac cgcctgtttc accacatacc cgaacgcaac
      301 aatgtttcaa cacacgggac ggcacatcaa gcaccgccct atgtgtcgtc ctgatttgga
      361 aggggttgca cccctcccga ataaagtctg atcctgccgc cccgaaggac ggatgtccga
      421 gtggcggggt ttcaaccgaa aaggaaatac aatgaaaatc aggccggggc ggcacaacgc
      481 gcccgacttt ccgcacgggg ccgccgtaac cataggcaat ttcgacggcg tacacctcgg
      541 acacaaacac atcctccaaa aactccgcct cgaagccgat acgcgcggat tgcccgtcgt
      601 ggccgtcgtt ttcgaacccc aacccaaaga atttttcgca ctccgtaccg gcaaaacccc
      661 gccctgccgt atcagccccc tgcgtaccaa actggaattg ctggaaggga cgggttgcgt
      721 cgatgccgcc tgggttttgc gtttcgatcg gaatttttcc gaaatatccg cgcaagcatt
      781 tatcgaccgc ctgctgcgtc aaaccttgaa tacgcgctat ttgctcgtcg gcgatgattt
      841 tcgtttcggc gcggggcgcg aaggctgttt tgaacttttg gcacaacagc ccgatatgca
      901 aaccgagcgc acgccttccg tcatcgtcga agacatccgc accagcagca ccaccgtccg
      961 ccaagccctt tcagacggca accttgccta tgcgaaaaaa cttttgggac acgactacgt
     1021 tttggggggc agggtggtgc acggcagaaa actcgggcgc accttaaacg ccccgaccgc
     1081 caacatccga ctgcccggcc accgttatgc actcggcggc gtgttcgtcg tcgaagcgga
     1141 cggcgcattc ggcacgcggc gcggcgtggc gagcttcggc ttcaatccca ccgttgatgg
     1201 cggctgttct caaaagcttg aagtccacct gttcgacttt caaggcgatt tgtacggaca
     1261 acggttgaac gtccgcttcc tgcacaaact gcgcgacgag gaaaagtttg acggtatgga
     1321 agaactgaaa aggcggattg aagccgatat ggaagccgca aagtgttggt agaaaaacct
     1381 tatacaaacc atccgattgg gctacaatca gccttttaac tgttcagacg gcacaggggt
     1441 ttcccgttgt gaaatactgt ttgaggcgca atgccgtctg aaaccgaaat attgtaacaa
     1501 tagagattaa aaaatgaccg attacagtaa aaccgtcaac ctgctcgaaa gcccgtttcc
     1561 gatgcgcggc aatcttgcca agcgcgagcc tgcgtggctg aaaagctggt acgagcaaaa
     1621 acgttaccaa aaactgcgcg aaatcgccaa aggccgtccg aaattcatcc tgcacgacgg
     1681 cccgccgtat gccaacggcg acatccatat cggtcatgcc gtcaataaaa ttcttaaaga
     1741 cattattatc cgcagcaaaa cccaagccgg ttttgacgcg ccttatgtac cgggttggga
     1801 ctgccacggc ctgcccatcg aagtgatggt ggaaaaactg cacggcaaag atatgcccaa
     1861 agcgcgtttc cgcgaattgt gccgcgaata tgccgccgaa cagattgccc gtcagaaaaa
     1921 agactttatc cgcttgggcg tgttgggcga ctgggacaat ccttacttga ccatggattt
     1981 caaaaccgaa gccgataccg tgcgtatgct cggcgaaatc tacaaatccg gctatctcta
     2041 ccggggcgcg aaaccggttc agttttgctt ggattgcggc tcttcgctgg cggaagcgga
     2101 agtggaatac aaagacaaag tatcgcctgc gattgatgtt gcctatccgt ttaaagacac
     2161 tgccgcgctt gccgccgcat tcggcttggc aggtatcgaa ggcaaagcgt ttgccgtcat
     2221 ttggacgacc acgccttgga ctctgcctgc gagccaggcc gtgtctgccg gcgcggacgt
     2281 ggtgtatcaa ttaatcgata cgcccaaagg caaattggtg ctggcgaaag atttggcgga
     2341 aggcgctttg aaacgctacg gcttttcaga cggcatcgcc atccttgccg aaaccaccgg
     2401 cgacaagctg gaaaacctgc acatgaatca tccgttcctc gaacgcgata ttcccatgct
     2461 caacggcgaa cacgttacca ccgatgccgg taccggcttg gtgcatactg cgcctgcgca
     2521 cggtttggaa gactacgccg tctgcaataa atacggcatc gagctttaca accctgtcaa
     2581 cgccgaaggc aaatacataa gcgaaacgcc tcgtgtcgca ggcatgagcg tttgggaggc
     2641 gaatcccgtc atcctgcaat ggccggaaga aaccggcaac ctcttggcaa gcagcaaaat
     2701 cgaacacagc tacgcccact gctggcgcca caaaaccccg ctgatttacc gagcgacagg
     2761 tcagtggttt gtcggcatgg acaaagccgg cagcgacggt aaaaccctgc gcgacaaagc
     2821 catcaaagcc gtggacgaca ccgaattctt cccgccatgg ggtcgtgcgc gtttggaatc
     2881 catgattgaa ggccgtcctg actgggtggt ttcacgccaa cgctattggg gcacgccgat
     2941 gactttcttt gttcacaaag aaacgggcga gctgcatccg aactctgccg aacttttgga
     3001 aaaagtcgcg caacgcatcg aagaaaaagg catcgaggct tggttctccc tcgataaaag
     3061 cgaattatta agcgcggaag attgcgaaca ttacgacaaa ctccccgata ccatggacgt
     3121 atggttcgac tcaggctcga cgcattattc cgttttgaaa cagcgcgaag aattggaatg
     3181 gccggctgac ttgtacctcg aaggcagcga ccaacaccgc ggctggttcc aatcctctat
     3241 gctgaccggt tgcgcctcat ccatgggacg cgcaccgtat aaacagctgc tgacccacgg
     3301 tttcgtggtt gaccaaaacg gccgcaaaat gtcgaaatcc atcggcaacg tcgtcgcgcc
     3361 gcaggaagtc tataacgagt tcggcgcgga catcctgcgc ctgtgggcgg catccaccga
     3421 ttacagcggc gaattggcga tttccaaaga aatcctcaaa cgcgtaaccg aaagctaccg
     3481 ccgtatccgc aataccttga gcttcctgtt tgccaacttg agcgatttca acccgattga
     3541 agatgccgtg caacaggcgg atatggtgga aatcgaccgc tacgccttgg tattggcgcg
     3601 gcggctgcaa gagcgtttgg caggcggtta ctatccgcgc tatgccttcc acttcgccgt
     3661 gaaagacatt gtttctttct gctcggaaga cttgggcgcg ttctacctcg acatcctgaa
     3721 agaccgcctc tacaccacca aagccgacag ccgcgcccgc cgcagcgcgc aaaccgccct
     3781 gtaccacatc acgcgcagcc tggttctctt gattgcaccg attttgtgct tcaccggcga
     3841 agaagcgtgg gacatcatcg gcggcggcga agaagacagc gtcctcttcc atacttggca
     3901 cgagttcccg gccatcaacg aaaaagccga agccgaactg gtgaaaaaat ggacggcaat
     3961 ccgcgaagcg cgcgaagcag taaccgccgc catcgagcct ttgcgcgccg acaaaaccgt
     4021 cggttcgtcc ttgcaggcgg aagccgaaat taccgcgccg gaagaaatgg ccggctatct
     4081 gaatgctttg ggcgaagaat tgcgctttgc cctgctggtg tctaaagcag aagtgaaagt
     4141 cggtgatgaa cttgccgttg ccgccaaagc cagcgacggc gaaaaatgcg aacgctgctg
     4201 gcattacacc cgcgatgtgg gcgcggttgc aggttatgaa accgtctgca aacgctgtgc
     4261 ggagaatgtc ggcggagaag gcgaaacgcg ccattacgcc tgataaagtt tgagcaaatg
     4321 ccgtctgaaa ccgccaaaca gtatttcaga cggcattttt tgtgccgcga tttgtcttca
     4381 taatggcgga ggggtttcga gattgcggtg ttgtcgggaa tgacggttca gttgctacgg
     4441 ttactgtcag gtttcggtta tgttggaatt tcgggaaact tatgaattgt cattcccgcg
     4501 aaagtgggaa tccaggactc aaaatctcaa gaaaccgttt tgcccgataa gttcctgcac
     4561 tgacagatct agattcccgc ctgcgcggga atgacgggtt tcgagattgc ggtgttgtcg
     4621 ggaatgacgg gttttaagat tacggtgttg tcggaacgca accgaaccgt cattcccacg
     4681 gaagtgggaa tctagaatct cggggtttca gtcatttccg atagattccc gccgcgtcgg
     4741 gggtccggat tcccgcctgc gcgggaatga cgggtttcga gattgcggtg ttgtcgggaa
     4801 tgatggaaaa tggcgggaat tgtgtaaaaa atgccgtctg aaaccgttgg aagcatcgta
     4861 aacgttggag tcgatgaatc ggtgggcttc agtccgccat tcccatcaat ccaacatttc
     4921 taccgttttc atcgaatcca tcgaatccgc ccctttcgac aatctgaccc tatgcaaccg
     4981 aaccgtcatt cccacggaag tgggaatcta ggacgcgggg tttcagtcat ttccgataga
     5041 ttcccgccgc gtcgggggtc cggattcccg cctgcgcggg aatgacgaat ttcgagattg
     5101 cggtattgtc gggaatgacg gttcgggtat ttccaagccc gccccgcgcc tgtaaacggc
     5161 gatcgcatca aaaatgccgt ctgaaggttc agacggcatc ggtgtcgggg aatcagcggg
     5221 ccgtagcaac cgaaccgtca ttcccacgga agtgggaatc taggacgcgg ggtttcagtt
     5281 atttccgata gattcccgcc gcgtcagggg tccggattcc cgcctgcgcg ggaatgacgg
     5341 gtttcgagat tgcggtgttg tcggacgggt ttcgagacta cggtgttgtc ggaacgcaac
     5401 cgaaccgtca ttcccacgga agtgggaatc taggacgcgg ggtttgggca actgttttta
     5461 tccgataagt ttccgtgcgg acaggtccgg attcccgcct gcgcgggaat gacgggtttc
     5521 gagattgcgg tgttgtcgga acgcaaccga accgtcattc ccacggaagt gggaatctag
     5581 gacgcggggt ttcagtcatt tccgatagat tcccgccgcg tcgggggtcc ggattcccgc
     5641 ctgcgcggga atgacgaatt tcgagattac ggtgttgtcg ggaatgacgg gtttcaagat
     5701 tacggtgttg tcggaacgca accgaaccgt cattcccgcg aaagtgggaa tccaggactg
     5761 aaaatctcaa gaaaccgttt tgcccgataa gttcctgcac tgacagatct agatttccgc
     5821 ctgcgcggga atgacgggtt tcgcgattgc ggtgttgtcg ggaatgacgg ttcgggtatt
     5881 ttactgcgcc cgccccgcgc ctgtaaacgg cgggcgcatc aaaaatgccg tctgaaggtt
     5941 cagacggcat cggtgtcggg gaatcagaag cggtagcgca tgcccaatga ggcttcgtgg
     6001 gttttgaagc gggtgttttc caagcgtccc cagttgtggt agcggtaccc ggcgtccagg
     6061 gtcaggttgg gcgtgatgtc gaaaccgacg ccggcgatga cgccgaggcc cacgcggcgg
     6121 atgctgtcgc tttcttgatg ggcgtcttgt gtctttggta ccctataaat cgtaggtgtt
     6181 gcgttaggag catgggggat ggtagtaacc tctattgttt t
//
LOCUS       DAXFZR010000103         6080 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000103 DAXFZR010000000
VERSION     DAXFZR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.104"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(304..540)
                     /locus_tag="ACFJAS_001936"
     CDS             complement(304..540)
                     /locus_tag="ACFJAS_001936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983099.1"
                     /translation="MKTANPHIVIPAKAALHTPPAAAIFTLKSRHSRESGNLGMKSSR
                     NLSEITETERTGFPPAREWRGKVGCGWDWWNDEI"
     gene            complement(544..699)
                     /locus_tag="ACFJAS_001937"
     CDS             complement(544..699)
                     /locus_tag="ACFJAS_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983100.1"
                     /translation="MPSESPSDGIGFPKHSAPFSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(716..2512)
                     /locus_tag="ACFJAS_001938"
     CDS             complement(716..2512)
                     /locus_tag="ACFJAS_001938"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC3983101.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTVVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEQDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSAISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIEWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2971..4224
                     /locus_tag="ACFJAS_001939"
     CDS             2971..4224
                     /locus_tag="ACFJAS_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC3983102.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QLALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSCVYLWVFLILCIASATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLVAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRIRPSGYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGRDKTGNAELFAWN
                     IWVAGSGLAVIFWFDGAMAELLKFAMIAAFVSAPVFAWLNYRLVKGDKRHRLTAGMNF
                     LAIVGLLYLAGFAVLFLLNLTGLLA"
     gene            4379..5890
                     /gene="lysS"
                     /locus_tag="ACFJAS_001940"
     CDS             4379..5890
                     /gene="lysS"
                     /locus_tag="ACFJAS_001940"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC3983103.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLNNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1301 a         1583 c         1781 g         1415 t
ORIGIN      
        1 aggtcgtctg aaaagcaaaa aacagcctgc acaacctgat tagtttacaa aacccttaac
       61 cccaacagcc tccccgtcct ctctcccgtg ggagagggct ggagagaggg caacaagccg
      121 caaggcttgt attggggcgg ttaagttgtt gggaaaggtt gccgcaactt ggagaatgcc
      181 gcctccccgg ccctccccca cgggggaggg agcagattgc ggcagatttg gcggttgcag
      241 gcggtttgat ttgcgaggga atcggacgcg caggcgggaa tccggatttg tccgcgcgga
      301 aacttatatt tcgtcattcc accaatccca cccacagccc acttttccgc gccattcccg
      361 cgcaggcggg aatccagtcc gttcggtttc ggttatttcc gataaattcc tgctgctttt
      421 cattcctaga ttcccacttt cgcgggaatg acgggatttc agggtaaaga tggcggcagc
      481 cggaggggtg tgaagagctg ctttcgcagg aatgacgata tgcggatttg ctgttttcat
      541 ccgctaccga tgccgtctga agcctgtttg gcgtttcaga cggcattggg ttttttgtgc
      601 atttgccgca aaacggatcg gtgtctgctt gcggcaagat gaaaacggcg cggaatgttt
      661 tgggaaacca atgccgtctg aagggctttc agacggcatt tttgtaccgt gccgtttaac
      721 gcgccagcgg ttcggtgcgt ttaatcagcc acgcttttgc cgcgccttcg gtcagcggct
      781 cgaggcggcg gcggacttcg gcgtggtagc ggttgaccca ttcgatttcg ccgtcggtca
      841 tcagggcggt gtccatcagg cgtgtatcga tggggcagag ggtcagggtt tcaaaacaga
      901 ggaagctgcc gaactcggtt tcctgcgggt cggcgacggc ttggttggcg gcgaggtttt
      961 caatgcggat gccccatttt cccggacggt agaggccggg ttcgatggag gtaaccatgc
     1021 cttttttcat ggcggtttcg ggcgtggcgg gggcggcgaa ggcgatgcgc tgcgggcctt
     1081 cgtggacgtt gaggaaatag cctacgccgt ggccggtgcc gtgtccgtag tcgcattgcg
     1141 cctgccacag gggtttgcgg caaatcgcgt caatcagcgg cgaggggatg ttttcgggga
     1201 acacggcttc ggcaagcgcg atatgggctt tgagaacgag cgtgttgtcg cgtttttgtt
     1261 ctgctgtggg cgtgccgacg gggacgacgc gggtgatgtc ggtcgtgccg cctttgtatt
     1321 gcgcgccgga gtcgatgagc agcagaccgt tgccgctgat ggcgctgtgg ctttcgggtg
     1381 ttgcgctgta atgcggcagg gcgccgttgg cgttgaagcc tgcgatggtg tcgaaactca
     1441 aggacacaaa acccgggcgc gcgctgcggt ggcggtaaag catcgtatcc acgtcgattt
     1501 cggtcaggct gccgcctttg ccgatgatgt cttcaaactc ggcgaagaag ccgcacaacg
     1561 ccgcgccgtc ttgttccatc gcttcgcgga tgcgggcgat gtcggcttcg gatttgacgg
     1621 atttgaagaa ggtggacggg ttgatgcctt caatcaggcg cgcgctttcg ggcaggcgca
     1681 cgagcgtact gacgacggtt ttgttcggct cgatgagcag cgcgccgccg atttgcgcga
     1741 gtttgtcggc aacttgggcg taaggctcga ccgtgatacc tgcggtttgc agcgcggcgg
     1801 cggcttcggc gttcagacgg cattgttcgg taaacaggac ggcactgtct ttgccgatca
     1861 gcaggaagga cacgaaaaca gggttgaaag gcacgtcgct gccgcgcagg ttggtcagcc
     1921 aagcgatgtc gtcaagcgag gaaaccaagt ggtaatccgc gcctttttcc gccataactg
     1981 cgcgcacgcg ggcgagtttc tcggcggcgg tttcggagac gtaggcgtgg tcgtggatga
     2041 acacggtttc ggcggggata gcgggacggc ttgtccacac ttggtcaagc aggtcgtcgg
     2101 ggtgttggat gcggatgttt ttggcggcga gcgattgcgc caaagtgcgt ttgccggtga
     2161 gcgacaccat atcggaaggg atgccgacgg cagcgttttc gggcaggttt gccgcgagcc
     2221 attcgttgta cggcggcact tgcccgcttt tttgcagctc aatgccgctg ccggaaagct
     2281 gtttggcggc ttgttcccaa tagcggctgt ccacccatac gcccgcttca tcggcggtaa
     2341 cgacgaatgt gccgaccgag ccggtaaagc ctgacaattc gcgccgcgcc tgccaatgct
     2401 cgggcaggta ttcggatagg tgggggtcgg cggaagggat gacgagtgcg tccaagcctt
     2461 gcgccttcat ggcttcgcgc aatgcggaca ggtaattcga tacggtattc ataacagttc
     2521 ctccaatcgg gtttcgcggt ttcagacggc atggacggaa atttgaaatg ccgtctgaaa
     2581 aacataacca tattatcaaa tcgccagcct gctgcaaaac ggacgggctg atcggatggt
     2641 gcgtgtttgc acgaaaaaaa gcgatgtaag gttgtttgtt gtaaaatttc aggcataaaa
     2701 tgtcgtttca tgccttcttt tggaagtaat acggaaaata tagtcaggtt tgagcatgaa
     2761 gttctaatag tgtgtgttgg caggtatttg gattacgtta caatacgcgc tttcatcaaa
     2821 atttccctac cgggcggtat cctgtttttg aagacggtag gatttgggtt tgtttggggt
     2881 caatcccgcc caaattggag caatagatag ggctgtgtcc gaatatgcgg ctctgtgtgt
     2941 tgaaacacag ttaaaaatac ggaagacttt atgtccgaac aacatatttc gacttggaaa
     3001 agcaagatca acgcattggg accggggatt atgatggctt cggcggctgt cggcggttcg
     3061 cacctgattg cctcgacgca ggcgggcgcg ctttacggct ggcagctcgc gctcatcatc
     3121 atcctgacca acctcttcaa atacccgttt ttccgcttca gcgcgcatta cactctggac
     3181 acgggcaaga gtctgattga aggttatgcc gagaaaagct gcgtttattt gtgggttttt
     3241 ttgattttgt gtatcgcctc cgccacgatt aacgcgggcg cggtcgccat tgtaaccgcc
     3301 gccatcgtca aaatggcgat tccctcgctg atgtttgatg ccggcacggt tgccgccttg
     3361 attatggcat cctgcctgat tattttggtg agcggacgtt accgcgcttt ggatcgcgtt
     3421 tccaaaatca tcatcgttac tttgagtatc gccacgcttg tcgccgccgg catcgctatg
     3481 tcgcgcggta tgcagatgca gcccgatttt atcgagccga caccgtggac gcttgccggt
     3541 ttgggcttcc tgatcgcgct gatgggttgg atgcccgcgc cgattgagat ttccgccatc
     3601 aattctttgt gggtaaccga aaaacaacgc atccggcctt ccggctaccg cgacggcatt
     3661 ttcgatttca acgtcggtta tatcgccagt gcggttttgg ctttggtttt ccttgcactg
     3721 ggcgcgtttg tgcaatacgg caacggcgaa gcagtgcaga tggcgggcgg caaatatatc
     3781 gggcaattga ttaatatgta tgccgtaacc atcggcggct ggtcgcgccc gctggtggcg
     3841 tttatcgcgt ttgcctgtat gtacggcacg acgattaccg ttgtggacgg ttatgcgcgt
     3901 gccattgccg aacccgtgcg cctgctgcgc ggcagggata aaaccggcaa cgccgagttg
     3961 tttgcctgga atatttgggt ggcgggcagc ggtttggcgg tgattttctg gtttgacggc
     4021 gcaatggcgg aactgctcaa atttgcgatg attgccgcct ttgtgtccgc ccccgtgttt
     4081 gcgtggttga attaccgtct ggtcaaagga gacaaacggc acaggcttac cgccggtatg
     4141 aatttccttg ccattgtcgg cttgctctac ctggccgggt ttgccgtttt gttcctgttg
     4201 aacctgacgg ggcttttggc ataggtttca aaatctgaat gccgtctgca agattttcag
     4261 acggcatcgc cctttcttgg aaaaaaacac cgacatcgcg taaaatacgc gcaaattttg
     4321 tgattcggtc aggtcgtctg aaacagactg cgcctgattt attttttcgg aaaaccctat
     4381 gagcgaacaa aaccatccgc aaaccgagcc gcagttggac gaaaaccaaa tcatcgccct
     4441 gcgccgcgaa aaactgaaca acatccgcca acagcgcaac gcttacccca acgatttcaa
     4501 gcgcgacagc tttgccgccg atttgcaggc gcaatacggc gaaatcggca aagaagaact
     4561 cgacccgcag gccgttcccg ttaaaattgc cggccgcatg atgctcaaac gtcaaatggg
     4621 caaagccagc ttcgccacca ttcaagacgt taccggtcaa atccagcttt atctgaacaa
     4681 caaaggcgtg agtcaggaag ttttggacga cttcaaccac tgggacttgg gcgacatcgt
     4741 cggtgcggaa ggcactttgt tcaaaaccaa ccacggcgaa ctaaccgtgc gtgtgtccga
     4801 catccgcctg ctgtccaaat ccctgcgtcc gctgcccgac aaacacaaag gcttgagcga
     4861 tcaggaaacc aaataccgcc aacgctacgt cgatttgatt gccaatgaag aatcgcgcaa
     4921 tacctttatc aaacgcagcc aaatcatcca atctgtgcgt aattttatgg tgggcgagca
     4981 ttatctcgaa gtcgaaaccc cgatgatgca cccgattccc ggcggcgcga cagcgaaacc
     5041 cttcgttacc caccacaatg ccttggacat tccgctctac ctgcgcatcg cgcccgagct
     5101 gtatctgaaa cgcttggtgg tcggcggctt ggaacgcgtg tttgaaatca accgcagctt
     5161 ccgtaacgag ggcatgtccg tgcgccacaa ccccgaattt acgatgattg aattttacga
     5221 agccttctcc gactacgaac gtatgatgca gatggcggaa gacatcatcc gcaacgcatc
     5281 gcgcacggta aacggcacgg caaacatcac ttacaacggc aaagaagtcg atttggaaag
     5341 cccgttcgaa cgtctgacca tcctcgaagc catcaaaaaa tacaatccgc actacaccga
     5401 cgagcagttg aacgatgcgg aatggctgaa aaaagaaatc gtcaaacacg gcgaaagcct
     5461 gccgccgtcc ccgggcatcg gcagcctgca actcgccctg tttgaaggtt gcgccgaggg
     5521 caaactgtgg aatccgacct ttattgtcga ttacccggtc gaagtttccc cgttggcgcg
     5581 cgcttcggat acgaaacaag gtctgaccga acgcttcgag cttttcgttg tcgggcgcga
     5641 actggcaaac ggctattccg agttgaacga ccccgaagac caagccgaac gcttcaaatc
     5701 gcaagtggcg caaaaagatg cgggcgacga cgaagccatg cactacgatg ccgactacat
     5761 ccgcgcgatg gaattcggtt tgccgccgac cggcggttgc ggcatcggca tcgaccgctt
     5821 agtgatgctg ctgaccgatt tgcaaaccat ccgcgatgtg attctgtttc cgcagatgcg
     5881 tccggagtaa tcatatattc aattaaaaac aaaatagtac aacactcgac gttgaaggtt
     5941 taaccatggc atactctgcg gacttaagaa acaaagcttt aaaccatagc ggattaacaa
     6001 aaatcaggac aaggcggcgg gccgcaggca gtacgaatgg tacggaaccg atccgcccgg
     6061 cgctccatca ccttagggaa
//
LOCUS       DAXFZR010000104         6019 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000104 DAXFZR010000000
VERSION     DAXFZR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.105"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            261..1670
                     /gene="leuC"
                     /locus_tag="ACFJAS_001941"
     CDS             261..1670
                     /gene="leuC"
                     /locus_tag="ACFJAS_001941"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC3983104.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            1765..2019
                     /locus_tag="ACFJAS_001942"
     CDS             1765..2019
                     /locus_tag="ACFJAS_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983105.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            2081..2722
                     /gene="leuD"
                     /locus_tag="ACFJAS_001943"
     CDS             2081..2722
                     /gene="leuD"
                     /locus_tag="ACFJAS_001943"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC3983106.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            2889..4160
                     /locus_tag="ACFJAS_001944"
     CDS             2889..4160
                     /locus_tag="ACFJAS_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3983107.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            4176..4907
                     /locus_tag="ACFJAS_001945"
     CDS             4176..4907
                     /locus_tag="ACFJAS_001945"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC3983108.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQIPPDFFLNKKDRSRELLEVKAFNRNACPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            4941..6011
                     /gene="leuB"
                     /locus_tag="ACFJAS_001946"
     CDS             4941..6011
                     /gene="leuB"
                     /locus_tag="ACFJAS_001946"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC3983109.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
BASE COUNT         1767 a         1554 c         1370 g         1328 t
ORIGIN      
        1 taatttcatc tttcgtctgc ctctgtgtgt tttttgacaa ggtacagata ataatgcttt
       61 tgaaagaatg acgacaagta ttctttcgta tcgaatcatt ctttagatat aacgaaatga
      121 acaatatgtc taattttttc gttaaaattg tttacaaaat aacaccgact caaaaattag
      181 acaaaatctg ttgcgcagta taaagaatac gtctaaaatc cgcccaatcc gcatcaccat
      241 ttatccaaaa gaacaacatc atgacggcac aaaccctcta cgacaaactc tggaacagcc
      301 acgtcgtccg cgaagaagaa gacggcaccg tcctgcttta catcgaccgc catctggttc
      361 acgaagtaac cagcccgcag gcgtttgagg gtctgaaaat ggcggggcgc aagctgtggc
      421 gcatcgacag cgtggtctcc accgccgacc acaacacccc gaccggcgat tgggacaaag
      481 gcatccaaga cccgatttcc aagctgcaag tcgatacgtt ggatcaaaac atcaaagagt
      541 ttggcgcact cgcctacttc ccgtttatgg acaaaggcca gggcatcgta cacgttatgg
      601 ggcccgaaca aggcgcgacc ctgcccggga tgaccgtcgt ctgcggcgat tcgcacactt
      661 ccacccacgg cgcattcggc gcactggcac acggcatcgg cacttccgaa gtcgaacaca
      721 cgatggcgac ccagtgcatt accgcgaaaa aatccaaatc tatgctgatt gccgttgacg
      781 gcaaattaaa agcgggcgtt accgccaaag acgtggcgct ctacatcatc gggcaaatcg
      841 gcacggcggg cggcacgggt tacgccgtcg agttcggcgg cgaagccatc cgcagccttt
      901 cgatggaagg caggatgacc ctgtgcaata tggcgattga ggcaggcgcg cgttccggta
      961 tggttgccgt cgatcaaacc accatcgact atgtcaaagg caagcccttc gcacccgaag
     1021 gcgaagcttg ggacaaagcc gtcgagtact ggcgcacgct ggtgtctgac gaaggcgcgg
     1081 tttttgataa agaataccgt ttcaacgccg aagacatcga accgcaagtt acttggggca
     1141 cgtcgcccga aatggtttta aacatcggcg gcaaagtgcc gaatcccgcc gaagaaaccg
     1201 atccggtcaa acgcagcggc atagagcgcg cactcgaata tatgggtttg aaggccggta
     1261 cgccgctgaa cgaaatccct gtcgatatcg tctttatcgg ctcttgcacc aacagccgca
     1321 tcgaagactt gcgcgaagcc gctgctatcg ccaaaggcca taaaaaagcc ggcaacgtac
     1381 agcgcgtgtt aatcgtcccc ggctccggtt tggttaaaga acaagccgaa aaagaaggct
     1441 tggacaaaat tttcatcgaa gccggttttg aatggcgcga accgggctgt tcgatgtgcc
     1501 ttgcgatgaa tgccgaccgc ctcgcgccga ggcaacgctg cgcctccacg tccaaccgca
     1561 attttgaagg ccgccaaggc aacggcgggc gcacccacct cgtcagcccc gctatggccg
     1621 ccgccgccgc cgttaccgga cactttaccg acatccgcac gatggcgtaa gtattttcag
     1681 gcagtcttta agaatttgac gaggccgtct gaaatatgtc ccgtaaggca gaaacaccta
     1741 atattcaaac caaggagttt aaccatgaac aaacttttcg ttaccgccct gtccgccctc
     1801 gccttgtccg cctgcgccgg cacttggcag ggcgcgaaac aagacaccgc ccgcaacctt
     1861 gacaaaacac aggccgccgc cgaacgcgcc gccgaacaaa caggcaacgc cgtcgaaaaa
     1921 ggttgggaca aaaccaaaga agccgtcaaa aaaggcggca atgccgtcgg acgcggcatt
     1981 tcccatctcg gcaaaaaaat cgaaaacgcc accgaataac cgatttgcga tgccgtctga
     2041 aaccgtttca gacggcatcg tcagcacaag acgcaaaccc atgaaagcct ttaccaaaat
     2101 caccgccatc gtcgccccgc tcgaccgcag caacgtcgat accgatgcca tcatccccaa
     2161 acaatttctg aaatccatca aacgcagcgg cttcggcccc aacgcctttg acgaatggcg
     2221 ttacctcgac cacggcgaac cgggcatgga caacggcaaa cgcccgttga accccgattt
     2281 ttccctgaac cagccgcgtt accaaggcgc gcaaatcctg ttgacgcgta aaaacttcgg
     2341 ttgcggctct tcacgcgaac atgccccttg ggcattggac gactacggct tccgcgccat
     2401 tatcgccccc agcttcgccg acatcttctt taacaactgc tacaaaaacg gccttttgcc
     2461 catcgtgttg accgaagaac aagtcgaccg gcttttcaaa gaagtcgaag ccaacgaagg
     2521 ctatcggctc tccatcgacc ttgccgagca aaccctgacc accccgggcg gcgaaacatt
     2581 cacattcgac attaccgaac accgcaaaca ctgcctctta aacggcttgg acgaaatcgg
     2641 actgaccctg caacacgccg acaaaattaa agcctttgaa gaaaaacgcc gccaaagcca
     2701 gccttggctg tttaacggtt aaaacctatg ccgtctgaag gctgttcaga cggcattcct
     2761 gataacatat aattgctgcg gctaatgtaa acagaggggc gataaattgt tttcatcttt
     2821 taagcgggct tagcaacact ctaaattaat gcaactctcc atttcaaata aatttttaga
     2881 tttttaatat gcaacaaatt aaatttattg acttattttc cggaatgagt ggcatcagga
     2941 aaggattcga acaagcctgt cggaaacagt cggttgcttg cgaatgtgtt tttacttcag
     3001 aaatcaaacc ggcagctttg gaagtattga agcaaaacta ccctgatgaa gtgccgtatg
     3061 gagatataac gaaaattgaa acaggggata ttcccgattt tgacatcctg ttggcaggct
     3121 tcccttgtca ggctttttct ttcgccggaa aaagattagg ctttgaagat acacggggaa
     3181 cgcttttctt tgatgtggca aggattttaa aggcaaaaaa accaaaaggt tttattttgg
     3241 aaaatgtgga aggattggtg acacacgata gaaaagattc gacacaaaaa atagggcgca
     3301 ccctgaccgt tattttggaa accttggaag cattgggcta ctatgtttct tggaaagttt
     3361 taaatgcaaa agatttcggc attccccaaa acaggaagcg catttatctg acaggcagtt
     3421 tgaaatccaa acccgactta tctttcgaaa caagcccaag tccgaaatta aaaaatattt
     3481 tggaatcggg actgcctact gaaagcagcc ctttcatcaa aaaattgcta aaaaaatttc
     3541 ccccgtccga actgtacgga aaatcagtaa aagacaaacg gggagggaaa aacaatattc
     3601 acagttggga tattgaatta aaaggcgcag taaccgaaga agagaagcaa ttgttaaata
     3661 tccttctaaa agaacggagg aaaaaaaaat gggcttcaga aatcggcata gattggatgg
     3721 atgggatgcc tttgacaaaa gcgcaaattt caactttcta taaacacccc gatctccaaa
     3781 atattttgga cagcctgaca gacaaaggct atttggtttt agagcatccg aaacaaaaaa
     3841 ttggcgggca aagaatcaaa gatgaatccc tgcccaaagg ctacaatatt gtttcaggta
     3901 aaaaatcttt tgaaatcaat aaaatattag atccaaacga tgttgcgcca accttggttg
     3961 caatggatat ggagcacctt ttcgtcgttg acaacggcgg tttgcgtaca cttaccggaa
     4021 aagaagggtt acgcttattc ggctatccgg acgattattc gtttgatatt cccaaaaaag
     4081 acagatgcga tttattgggt aataccgttg ccgtccctgt gattaaggcg gtatctgaaa
     4141 gacttctgca tactttataa accgacaggg ataaaatgat aaaacttact gcacaacaaa
     4201 tatttgataa gttgctggat gaagaaaaaa tcttatcggc caatggtcaa attagatttt
     4261 tcttaggaga tgtggatatt atcgtcaaac aaaaagatgt tgtcggcaac atcattcagg
     4321 aatggctcgg cggatggttg agaaaaaggg aaattgaatt tgatgtttca accaataccc
     4381 aaatacctcc cgatttcttt ttaaataaaa aagaccgcag cagagaattg ttggaagtaa
     4441 aggcgttcaa ccgaaatgcc tgcccgggtt ttgatattgc agattttaaa atgtattctg
     4501 atgaaatcat tcataagccc tatatgctgg atgtagatta tttaatattc ggttacgata
     4561 tggacgacaa cggcaatgta accatcaagg atttatggct taaaaaagta tggcaaatta
     4621 ccagaagcat ggatgggtgg gcaattaatc ttcaagtcaa aaaaggcgtg gtgcataaaa
     4681 tccgcccggg tgtttggtac agcataaata aaaagaatat gcctatgttt gaatgcttgg
     4741 aagattttgt ttccgcaatt gaagaaaccg tttatcaaaa cccggcaaca cggcataacg
     4801 catccttatg gaaaaagaaa tttgaggaag cttataaaaa acactacaac cgatcgattt
     4861 ccataccccg ttggcacgaa attgcacaca aatacaaaaa gaaataaccc ttcaaccatc
     4921 aacccataag gaaacttgaa atgaccaaac atatcgccat cctccgaggc gacggcatcg
     4981 gtcccgaaat cgtcgccgaa accgtccgcg tactcgacaa atttatcgcc caaggcttgg
     5041 atgccgacta cgaatacgcg cctttgggcg gcgaagccta cgacgaatac ggccatcctt
     5101 atccagaatt tacgcaaaac ctctgccgca aagccgatgc cgtcctgctt ggtgcagtcg
     5161 gttcgcccca atacgacaat ctcgaccgtc cgttgcgccc cgagcgcggc ttgttagcaa
     5221 ttcgtaagga tttgaacctg tttgccaatt tgcgtcctgc cgttttatat cccgaattag
     5281 ccaacgcctc cacgctcaag cctgaaatcg ttgccggttt ggatattttg attgtgcgcg
     5341 aacttaccgg cgatatttat ttcggcgaac cgcgcggcat ccgtgttttg gaaaacggcg
     5401 aacacgaagg ctacaacacc atgaaataca gcgaaagcga aatccgccgc atcgcccacg
     5461 tggccttcca atccgcccaa aaacgcagca aaaaagtctg ctccgtaggc aaagccaacg
     5521 ttttggaaac caccgaactg tggcgcgaaa tctttgaaga aatcggcaaa caatatcccg
     5581 atgtcgagct ttcccatatg tatgtggaca atgccgccat gcaactcgtg cgcgcgccca
     5641 aacagtttga cgtgattgcc accggcaaca tcttcggcga catcctctcc gacgaagcct
     5701 ccatgctgac cggctccatc ggtatgctgc cttccgcttc tttggacgaa aacggcaaag
     5761 gcctgtacga accgtctcac ggctccgccc ccgacatcgc cggacaaaac aaagccaacc
     5821 cgctggccac catcctctcg cttgccatgc tgctgcgtta cagcctgaac gacgaagcgc
     5881 gcgcgcaaca agtcgaaaac gccgtccaaa aagtgctgca acaaggcttg cgtaccggcg
     5941 atatttacga agaaggcaca aaactcgttt cctgctccga aatgggcgac gcggtactcg
     6001 ccgccttgta aaaaacaag
//
LOCUS       DAXFZR010000105         5934 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000105 DAXFZR010000000
VERSION     DAXFZR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.106"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..3179)
                     /locus_tag="ACFJAS_001947"
     CDS             complement(<1..3179)
                     /locus_tag="ACFJAS_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC3983110.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLTYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAGYEQIHRALLTGL
                     IANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLAVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     DFYMADSVSEGLCPANPQQTTPSPVREGWGEGKTVAAQTNFSATSASPLPNPLPQERE
                     QSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPPFSDGLRPANPQQ
                     TTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDDPKPKKQPASQKG
                     RLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEEQFPKHWQTADGK
                     FKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQLQIKALPKQIRR
                     ICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQDEWAAFRLPEHC
                     YFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDNVTAWDIGILPES
                     IKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIELMKLQLKEQVKDL
                     NKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEKAFKEQIKRARSR
                     LPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPGFATRTPWAQWPR
                     LPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQGQPVSDGLAAFK
                     WMIEELRVSLFAQELKTPYPVSVKRLLKM"
     gene            complement(3281..4858)
                     /gene="lpt3"
                     /locus_tag="ACFJAS_001948"
     CDS             complement(3281..4858)
                     /gene="lpt3"
                     /locus_tag="ACFJAS_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC3983111.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSMIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIADK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRNGKAEYVYPQ"
     gene            5319..5471
                     /locus_tag="ACFJAS_001949"
     CDS             5319..5471
                     /locus_tag="ACFJAS_001949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983112.1"
                     /translation="MSNKQANHQSNMSNANKGTSGTNKTYSQNQGNRGWQMNPQNPAN
                     SGKGKK"
     gene            5542..>5934
                     /locus_tag="ACFJAS_001950"
     CDS             5542..>5934
                     /locus_tag="ACFJAS_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983113.1"
                     /translation="MENKQTEKTETQSEKTKKKPFKQTRELVRLALNNGWTQTEIAKT
                     CRTQQSVVSAWSKGEKLGTEQQLKPLLDLFGYKLRRNTFKLYWRKNAETSKIEFIKVE
                     GKIIFSLLIGKNNGYNKFIPAYKFIIHYQ"
BASE COUNT         1377 a         1341 c         1662 g         1554 t
ORIGIN      
        1 cacatcttca acagccgctt taccgacacc ggatacggcg tcttcaactc ctgcgcgaac
       61 agcgacactc tcaattcttc aatcatccat ttaaacgcgg cgaggccgtc tgaaacgggc
      121 tgtccttgtt tcgccaagcc gtcagttttt tcctgccaca tttgttccag ctcttggata
      181 tcggcttcgc gggctgcgtc gcgggcgggg ttgctgctgt atttttcgag gcgcagggtc
      241 atggctttga ggtagatggg gaggcgcggc cattgtgccc acggggtgcg ggtggcgaag
      301 ccgggtgcaa gcagggtttg caggcgtagt cttaggaggt gggtcaatgg gtgtttgccg
      361 agtttgccgt tcagttcggc gtaggcggct gcggtttcct gcaggtagcg gctgagggct
      421 tctttgacgg cgggcaggcg gctgcgggcg cgtttgattt gttctttgaa ggctttttcg
      481 ttgcgcggca gcttgtcttc gccgataaag gcgcggtcgc agacggcttg ggtgaggtcg
      541 tcgcgcaggg tgtcggcgtt gatgtgtttg agcagcatgg cggcttgggt gaagccttgg
      601 atgcctttgt tcagatcttt aacttgttct tttagttgta gtttcatgag ttcaatcacg
      661 cctaatctgt gggcgtgtcc ggcggcggca gacgtgtcga acaggcgcag ggcgatgcgg
      721 ccgtcttttt ctttttgcag gccgaggtag ccggtgagct gttgtttgcc gcgggcgaat
      781 ttgatggatt cgggcaggat gccgatgtcc catgcggtaa cgttgtcacg ctcgaattct
      841 tgggtgttgt cgcggaaggt ggtggtggcg gctttaccaa gttgttgttg gatttggatt
      901 aaatcgcgac ccatggctaa ctcttgtccg ccgtcgtcga tgatgcggag gttgaaatag
      961 cagtgttcgg gcagcctgaa cgcggcccat tcgtcttggt tgatttgctc gagtatgcgg
     1021 atgtcgcctg cggttttggc gatggcttgg gcgagctggg gcaggatggg ggcgttgcgg
     1081 tcggggtttt ggcttaaaaa ttgggtgatg aattcgggta cgggtacgca gatgcggcgg
     1141 atttgtttgg gcagtgcttt gatttgtagc tggatttttt cgcgtatcat gccgggcacg
     1201 agccattcga gggcggcggg gctgatgcgg ttgagcacgg tgagcggcag ggtgagggta
     1261 acgccgtcga gcgggtggtg cggctcgaag cggtagctga gtttgaattt gccgtctgcg
     1321 gtttgccagt gtttggggaa ttgttcttcg gtgatgtgcg cggcggcgtg ctgcatgagg
     1381 tcgtcgcggc tgaggaacaa caggcgcggg ttgtcgcgct cggcggtttt gagccaggct
     1441 tcaaaggtgc ggatatcggc aaggggcaaa ggtttcagac ggcctttttg agatgcaggc
     1501 tgctttttgg gtttggggtc atctgaaact gttaaagccg aggcactctg ttccctctcc
     1561 tgtgggagag ggtttgctgc ggttgcggaa aagttggttt gtgcggcaac tgttttgccc
     1621 tctccccagc cctcccccac gggggagggg gtggtttgct gggggtttgc aggacgcagg
     1681 ccgtctgaaa atggcggggt gtagttttca gatagccttt gggagtgcag gctgctttcg
     1741 gttttagttt tagcttcgca gaaattcagc cttgcttcgc aagacattgt tttcagactg
     1801 cctgaaaccg ttgaaactga ggcactctgt tccctctcct gtgggagagg gttagggaga
     1861 gggcttgctg aggttgcgga aaagttggtt tgtgcggcaa ctgttttgcc ctctccccag
     1921 ccttccctca cgggggaggg ggtggtttgc tgcggatttg caggacacag gccctctgaa
     1981 accgaatccg ccatataaaa atcgggcagc cgttcgtgat aaaacgcaaa cagggcttcg
     2041 tcatcgacca atacgtcttg cttgcgcgat ttgtgttcga gttcggtaat ttctttaatc
     2101 agctttttgt tgtggacaaa aaaatccgct ttcaaatcgt attcctgcgc caccaacgcg
     2161 ctgcggataa agatttcacg tgcttcttcg ggcgcaactt taccgtaaga cacggggcgg
     2221 cgcggcaata cggccaggcc gtaaagcgtt acgcgttcgc tggcgacgac ttcgccgcgt
     2281 ttttgttccc agtgcggctc gaaataatgg tagcggacaa ggtgcggggc ttcctgctcg
     2341 atccattcgg gctggataac ggcgacgtcg cgcgcgtaaa ggcgcgtggt ttcaaccaat
     2401 tctgccgcca tcacccattt gggcttggct ttgaacaggg cggaggcggg gaaaaggtgg
     2461 aagcggctgc cgcgcgcgcc ggtgtagtcg ttgctgtcgg gcgatttcat gccgacgttg
     2521 gcgatgaggc cggtgagcag ggcgcggtgg atttgttcgt agcccgcttc tttggtagcg
     2581 cggatttggg tgcggtgttg tttcttatcc agttgttttt gtttgagttt ggcagatagg
     2641 tcttggtcgc ccgcattctc agacgacgtc agttgcttga cttcgggagg tcgtctaaac
     2701 gcagcttctt tggtggtcaa gcccatttca atcgcggttt gggcaagctg gtggtgcagc
     2761 tcgcgccact cgcgcatcct caggtgcgac agaaaatatt gacggcacca ttgcaccaac
     2821 tgcttgttgg acaaaccttt gtcgcgctcg cgctggaaac tgtcccaaat attcaggtag
     2881 gtaaggaaat cggattgctt gtcggtaaag cgttcatgcg ccttggccgc ggcatcgcgc
     2941 gcttccagcg gccgttcgcg cgggtcttga atcgacagcg cagacgcaat caccaatatt
     3001 tccgccatgc agtcgtgttt cttcgccgcc agcaaaatgc gcgcaatttt cggatcgatg
     3061 ggcaggcgcg ccatttgttc gccgagcttg gtcaggcggt agcgcgtgtt ggtaccgata
     3121 tggcccgaac tgttttgcag gccgtctgaa acattggccg ggttggatcg ggcatccatg
     3181 cctgatccgc gtacttggtt tttcatattt tgcatagtct tgtcggtcgg gcatcttccc
     3241 cgacatcatc taaatttgtc tttattggtt ttcacgctgc ttattgcgga taaacatatt
     3301 ccgccttgcc gttgcgaatg ttcaagctgc ctgcatcgcc cgtaatcagg ttgcctgtta
     3361 ccgagccttc gcgacaacct gaaaccggca tatcgtagcc caacgtgtga atcaggaacg
     3421 ttgaaagctg ctgatggaag gcaatctcgc aaggcgcaaa agcctggttg gcagcctgtt
     3481 gcacggcctt atccgggctg tacaaaacca gaggcacaat atagctgtcg ggctgcaccg
     3541 tgccttgatt gtagatatct tggcgcacat actggccatg atcggaggta taggcaaaca
     3601 gccagttgcc gtcaggctgc ttttgcagct gctcgaatac ggtttgaatc atttggtcgg
     3661 ttttgtggat ggtgttgtcg tacttatccg caatatcggc ttcgccgaat actttatctt
     3721 gaggctgcaa caatgcgccg tatggggcgt gcgaaccgcg ttggtgcaac acgataaaat
     3781 gcctgccctg ctgcaaattg attttgtcga acaacggcag cagcttctca tcgggcatat
     3841 tgtcgccgtt gccgtagcca agttgcgtcg gctgaatcag atggtctatc catttcttac
     3901 cgattaagtt caaaattgcc atttggtttt cagcctgggc actgtaaaaa tacgtttcat
     3961 agccctgctc tttggcgagg cggaacatat tggtatcgcc gccgctgatt tgttccaagc
     4021 cgttggcgtg cggtatgacg ttaaagaaac tgggcaggga tactgccgtc ataaagcctg
     4081 cggaataact ttgtttcaca atcggcttaa aatcggcttg cgacagccgg gttaaaaacg
     4141 gcgaagtttc gcgcccgtaa ccaaacaatt tcaaatgcgc cgcgctttcg ctttcgccca
     4201 taatcaggac gatattttga atactgcctt gcccgatttt gcttggagca ggctgtttga
     4261 acacagggat cttgcttaaa tcaaacaact gatacggcaa cacgcgtccg acaaaataac
     4321 cgaagctgaa ataattggct ttgatgcggc tgtatgtcgg tttgggcgaa ataccgtgct
     4381 cttgtttcgt gtcgaacgaa cgcacgaaaa tcatcagcat taggaaggca aacaggatat
     4441 cggcagaaaa atgcgtctta cggcggaact tggcaaggct gcaaaacagc acgacttccg
     4501 ccacgcccca caaagcaggc agccacaact tatccaacat cgacgcgccc gcgctgccga
     4561 cttcggtaac ctctttcagc atcagccaat aattgatgcc cgtcatccag ctttgataaa
     4621 ccgcgtaatg cacattgttg gcaatcatgc tgaacgcgaa aaacaccgca atcagcagcc
     4681 gcgaagcctt ataacgcgca aacagataca gcgcagcaat cataaatgtc agcgcaaacg
     4741 tttccgccat ttttgcagcc ggtaaggttt caattccgaa tacaaagcga taggcgattt
     4801 cgctggcggt aagtagggat gaatacagaa agagaacgaa aagggatttt ttcataatgg
     4861 gaagttcaca ataaaagttg caggacgggt tttggggctc ggcaacggta ttcttcagca
     4921 gctatctgtt tacagattgt ttttcaaaaa acgagaagta aatcttaaat ccatagaatg
     4981 tctatcttaa aatcattaaa tatcaataaa ttacaaaata tcataaaata tcaaaattta
     5041 tcaacttttt atcaatggaa cttttgctac gaatttttta tgatgctacc tatcgtcatg
     5101 gggggtaatc atgaaaaaat ttagcaccaa ttcaaaaatc aattctttta ttcattcact
     5161 actaaatagg gggtggaaaa tacaaaatag aaaaaagcac aatattttgg tagcaccaaa
     5221 taaccgtaga attgcaatcc catcaacacc gagcgattgc aaagcaatcc acagtttcac
     5281 caagcagatt aaatatcttt ttcaggagta gaactaaaat gagcaataaa caagccaatc
     5341 atcaatcaaa catgagcaat gctaataaag gcacgtcagg aacaaataaa acgtattcac
     5401 aaaatcaggg aaatcgtggc tggcaaatga atccacaaaa tcctgcgaat agcggtaaag
     5461 gcaaaaaata gccattgcct tgcctataaa tggcgatatt atacttggta tcgccatttt
     5521 aattggcata ttgggggtat tatggaaaac aaacaaaccg aaaagacaga aacacaatct
     5581 gaaaagacaa agaaaaaacc atttaagcag acgagagaac tcgttaggct ggcattgaat
     5641 aatggttgga cgcaaactga aattgctaaa acttgccgca cacaacaatc agttgtgagt
     5701 gcatggagca agggcgaaaa attgggaaca gagcaacagc tcaaaccttt attggattta
     5761 ttcggataca aattacgtag gaacacattc aagctgtatt ggcgcaaaaa tgcagagaca
     5821 tctaaaattg aatttattaa agtagaagga aaaataattt ttagtcttct tattggtaaa
     5881 aacaatggct ataataaatt tataccagca tataaattta tcattcatta tcaa
//
LOCUS       DAXFZR010000106         5840 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000106 DAXFZR010000000
VERSION     DAXFZR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.107"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            64..1590
                     /gene="putP"
                     /locus_tag="ACFJAS_001951"
     CDS             64..1590
                     /gene="putP"
                     /locus_tag="ACFJAS_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC3983114.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            1842..5447
                     /gene="putA"
                     /locus_tag="ACFJAS_001952"
     CDS             1842..5447
                     /gene="putA"
                     /locus_tag="ACFJAS_001952"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC3983115.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAAAGIRTVVEPGSPLASYTADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSVNTTAAGGNAGLMAVAD"
BASE COUNT         1298 a         1809 c         1511 g         1222 t
ORIGIN      
        1 caggcgcaag ttgcttgcaa tttcaaagcc tgtaattctg aagttttaga cgaggaatag
       61 cacatgaatc ccatgtacat cacttttgca atctatttgg ttgcagtcct tctcatcggg
      121 cttgccgctt atttttccac gcgcaatttc gatgattata ttttgggcgg gcgcagcctg
      181 ggcccgtttg ttacggcgat gtcggcaggc gcgtccgata tgtccggctg gcttttgatg
      241 ggtctgccgg gcgcgattta tttgagcggt ttgaatgagg cttggattgc catcggcctc
      301 ttggtcggcg cgtatttcaa ctggctcttg gtggcgggcc gcctgcgcgt gcataccgaa
      361 tatgccaaca atgcgctgac gctgccggat tatttcttcc accgcttcgg cgcgggcgga
      421 cacttgatga aagtggtttc cgcactgatt atcctgtttt tcttcacgat ttattgcgcc
      481 tcgggcattg tggcgggcgc aaccctgttc caaagcctgt ttgaaggtat gacttacaat
      541 caggcaatgt ggctgggcgc gggcgcgacc atcgcctata ccttcttggg cggcttcttg
      601 gcggtcagct ggacggatac gctgcaggct tctttgatga ttttcgccct gattttaacg
      661 ccggtgatgg tctatctggg cttgggcggc gcggaacaga tgtctgccgc gattcaaagc
      721 gtcgccgcag gcacgggcaa agaatacggc agcctgtttg ccggtacgac cgtcatcggc
      781 atcatttcca ccgccgcctg gggcttgggc tatttcggac agccgcacat tttggcgcgc
      841 tttatggccg ccgaaagcgc gaaatcgctg gtatccgcac gccgcatcgg catgacttgg
      901 atggcgttgt gccttgcggg cgcggtagcg gtcggttatt tcggcattgc gtatttcggt
      961 gcaaaccccg acaaagtttc ttctatgagc ggcaaccacg aacgcatctt catcgcgctt
     1021 tccaccctgc tgttcaaccc ttggatcgcc ggcatcattt tgagcgcgat tttggcggcg
     1081 gtgatgtcca ccctgtcctg ccagcttttg gtttgctcca gcgcgattac cgaagacttc
     1141 tacaaaggct ttttgcgtaa aaacgcgcaa cagtcggaat tggtatgggt cggccgcctg
     1201 atggtattgg cgattgccgt gatttccatc ctgattgctt ccgatccgaa cagcaaagta
     1261 ttgggactgg tgtcttacgc gtgggcaggt ttcggtgcgg cattcggccc gattgtgatt
     1321 ctgtccgtat tgtggaaacg catcactgcc tacggcgcgt tgtcaggcat ggttgcaggt
     1381 gcgtcaaccg ttgttgtgtg ggcggaatgg gtaaaaaaac cggctcgagc ggcaggcgaa
     1441 agcggcttgt tgacgatgta cgaaatcgtc ccgggcttta ttgtttgctt gatcgttgcc
     1501 gtgttggttt ccctgttcaa caaagaacct tcgcgcgaaa tccaagaacg ctttgaaaaa
     1561 gccgacgcag attaccgcgc cgcccgataa acccctttca gacggcacgg catcccggtt
     1621 cgtgccgtct gaacccgctt ccgggccaaa cacaaataaa ccttgcctgc atacaggcct
     1681 tatttgtgtt ttacccgttt cattttcaaa gaaacggaag caccgattct gcccgcattt
     1741 gaagccttgt atttcatctg cggatttatt acgatattac cgtattcagg ccgcaccgat
     1801 gccgtctgaa ccccccgaaa aaactttgga ggatccaaaa aatgtttcat tttgcatttc
     1861 cggcacaaac tgccctgcgc caagcgataa ccgatgccta ccgccgtaat gaaatcgaag
     1921 ccgtacagga tatgttgcaa cgtgcacaga tgagtgacga agagcgcaac gccgcctccg
     1981 agcttgcccg ccgtttggtt acccaagtcc gcgccggccg caccaaagcc ggcggcgtgg
     2041 atgcgctgat gcacgagttt tcactctcca gcgaagaagg catcgcgctg atgtgtctgg
     2101 cagaagccct gctgcgtatc cccgacaacg ccacgcgcga ccgcctgatt gccgacaaga
     2161 tttcagacgg caactggaaa agccatttga acaacagccc ttccctcttc gtcaatgctg
     2221 ccgcctgggg tctgctgatt accggcaagc tgaccgccac aaacgacaaa caaatgagtt
     2281 ccgcactcgg ccgcctgatc ggcaaaggcg gcgcaccgct catccgccaa ggcgtaaatt
     2341 acgccatgcg gcttctgggc aaacagttcg tcaccggaca gaccattgaa gaagccctgc
     2401 aaaacggcaa agaacgcgaa aaaatgggct accgcttctc cttcgatatg ttgggcgaag
     2461 ccgcctacac ccaagccgat gccgaccgct actaccgcga ctatgtcgaa gccatccacg
     2521 ccatcggcaa agatgcggca ggacaaggcg tttacgaagg caacggcatt tccgtcaaac
     2581 tctccgccat ccatccgcgc tactcgcgcg cccaacacgg ccgcgtgatg ggcgaactgt
     2641 tgcctcgcct gaaagaattg ttccttttgg gtaaaaaata cgatatcggt atcaacatcg
     2701 atgccgaaga agccaaccgt ctggagctgt ctttggattt gatggaggca ctcgtttccg
     2761 accccgattt ggccggctac aaaggcatcg gtttcgttgt tcaagcctac caaaaacgct
     2821 gcccgttcgt catcgactat ctgattgacc tggcccgccg caacaaccaa aaactgatga
     2881 tccgcctcgt caaaggcgcg tattgggaca gcgaaatcaa atgggcgcaa gtggacggct
     2941 tgaacggcta tccgacctac acccgcaaag tgcataccga catctcctac ctcgcctgcg
     3001 cgcgcaaact gctttccgcg caagacgcgg tattcccgca attcgccacc cacaacgcct
     3061 acaccttggg cgcaatctac caaatgggca aaggcaaaga ttttgaacac caatgcctgc
     3121 acggtatggg cgaaaccttg tacgaccaag tcgtcggtcc gcaaaactta ggccgccgcg
     3181 tgcgcgtgta cgccccggtc ggcacacacg aaaccctgct cgcctacttg gtgcgccgcc
     3241 tgttggaaaa cggcgcgaac tcgtctttcg tcaaccaaat cgttgatgaa aacatcagca
     3301 tcgaccgcct gattaagagc ccgttcgaca ccatcgccga acaaggcatc cacctgcaca
     3361 acgccctgcc gctgccgcgc gatttgtacg gcaaatgccg tctgaactcg caaggcgtgg
     3421 acttgagcaa cgaaaacgta ttgcagcagc ttcaagaaca gatgaacaaa gccgccgcac
     3481 aagacttcca cgccgcatcc atcgtcaacg gcaaagcccg cgatgtcggc gaagcgcaac
     3541 cgattaaaaa ccctgccgac cacggcgatg ttgtcggtac ggtcagcttt gccgatgccg
     3601 cgcttgccca agaagcggtt ggtgcagccg ttgccgcgtt ccccgaatgg agtgcgacac
     3661 ctgccgccga acgcgccgcc tgcctgcgcc gtttcgccga cctgctcgaa cagcacaccc
     3721 ccgcgctgat gatgcttgcc gtgcgcgaag cgggcaaaac gctgaacaac gccattgccg
     3781 aagtgcgcga agccgtcgat ttctgccgct actacgcaaa cgaagccgaa cacaccctgc
     3841 ctcaagacgc aaaagccgtc ggcgcgattg tcgccatcag cccgtggaac ttcccgctcg
     3901 ccatctttac cggcgaagtc gtgtccgccc tggcggcagg caacaccgtc atcgccaaac
     3961 ctgccgaaca aaccagcctg attgccggtt atgccgtttc cctcatgcac gaagccggca
     4021 tcccgacttc cgccctgcaa ctcatcctcg gcgcaggcga cacgggtgcg gcattgacca
     4081 acgatgccag catcggcggc gtgattttca caggctcgac cgaagtggcg cgcctgatca
     4141 acaaagccct tgccaaacgc ggcgacaatc ccgtcctgat tgccgaaacc ggcggacaaa
     4201 acgccatgat tgtcgattcc accgcacttg ccgaacaagt ctgcgccgac gtattgaact
     4261 ccgccttcga cagcgcggga caacgctgct ccgccctgcg tatcttatgc gtccaagaag
     4321 acgttgccga ccgtatgctc gacatgatca aaggcgcgat ggacgaactc gtcgtcggca
     4381 aaccgattca gctcactacc gatgtcggtc ccgtcatcga tgccgaagcg cagcaaaacc
     4441 tgttgaacca catcaacaaa atgaaaggcg ttgccaagtc ttaccacgaa gtcaaagccg
     4501 ccgccgatgt cgattccgaa aaatccacgt tcgttcgccc catcctgttt gaattgaaca
     4561 acctcaacga actgcaacgc gaagtcttcg gccccgtcct gcacgtcgtc cgctaccgcg
     4621 ccgacgaact cgacagcgtc atcgaccaaa tcaacagcaa aggctacgcc ctgacccacg
     4681 gcgtacacag ccgcatcgaa ggcacggtac gccacatccg cagccgcatc gaagccggca
     4741 acgtttacgt caaccgcaac atcgtcggtg cagtcgtcgg cgtacagccc ttcggcggac
     4801 acggtctgtc cggcacaggc cccaaagcag gaggttcgtt ctacctgcaa aaactgaccc
     4861 gcatccccga atgggttgcc ccgaccctga gccaaatcgg acaggcagac gaagccgcgc
     4921 tcaaacgcct cgaagccctg attcacaaac tgccgttcaa cgccgaagag aaaaaagccg
     4981 cagcggccgc tttgggacac gcccgcatcc gcaccctgcg ccgtgccgaa accgtcctta
     5041 ccggcccgac cggcgagcgc aacagcatct catggcacgc gccaaaacgc gtctgggtac
     5101 acggcggcag cctcctgcag gccttctgcg ccctgaccga acttgccgcc gccggcatcc
     5161 gaaccgtcgt cgaacccggc agcccgttgg cttcctacac cgccgacttg gacggtttgc
     5221 tgcaaatcag cggcaaaccc gaaaacaccg gtgtgaacca cgtcgccgcc ctaagcccac
     5281 tggacagcag ccgcaagcag gaactggcaa accgcgacgg cgcactcatc cgcatcctcc
     5341 cgtccggaca aggcttggac atcctgcaag tgtttgaaga aatttcttgc agcgtcaaca
     5401 ccacagccgc cggcggcaac gccggcctga tggcggttgc cgactgattt tgccaaaccg
     5461 cccgggcgcg gcccgtgaac aaatgccgtc tgaaaacctt tcaggcggca ttttatagtg
     5521 gattaacaaa aatcaggaca aggcggcgag ccgcagacag tacaaatagt acggcaaggc
     5581 gggccaacgc tgtactggtt taaattcaat tcactatatg tgttggcaca tcgctccaaa
     5641 cctgatataa tccgcctttc aatatcagtg aaaatcgttc tttttagtca gttaacatta
     5701 aatttcggag tcgaaaaatg aatccagccc caaaaaactt ctcttctctt ctcttctctt
     5761 ctcttctctt ctcttctctt ctcttctctt ctcttccgca gcgcaggcgg caagtgaagg
     5821 caatggccgc ggcccgtatg
//
LOCUS       DAXFZR010000107         5800 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000107 DAXFZR010000000
VERSION     DAXFZR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.108"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            194..391
                     /locus_tag="ACFJAS_001953"
                     /pseudo
     CDS             194..391
                     /locus_tag="ACFJAS_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            452..1486
                     /gene="pyrC"
                     /locus_tag="ACFJAS_001954"
     CDS             452..1486
                     /gene="pyrC"
                     /locus_tag="ACFJAS_001954"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC3983116.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(1565..1990)
                     /gene="nusB"
                     /locus_tag="ACFJAS_001955"
     CDS             complement(1565..1990)
                     /gene="nusB"
                     /locus_tag="ACFJAS_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC3983117.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(2068..2544)
                     /gene="ribH"
                     /locus_tag="ACFJAS_001956"
     CDS             complement(2068..2544)
                     /gene="ribH"
                     /locus_tag="ACFJAS_001956"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC3983118.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(2591..2914)
                     /locus_tag="ACFJAS_001957"
     CDS             complement(2591..2914)
                     /locus_tag="ACFJAS_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC3983119.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            3083..3802
                     /gene="rnc"
                     /locus_tag="ACFJAS_001958"
     CDS             3083..3802
                     /gene="rnc"
                     /locus_tag="ACFJAS_001958"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC3983120.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHADDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            3849..4772
                     /gene="era"
                     /locus_tag="ACFJAS_001959"
     CDS             3849..4772
                     /gene="era"
                     /locus_tag="ACFJAS_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC3983121.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            5336..>5800
                     /locus_tag="ACFJAS_001960"
     CDS             5336..>5800
                     /locus_tag="ACFJAS_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding N-lobe domain-containing
                     protein"
                     /protein_id="HFC3983122.1"
                     /translation="MRKLNYYGIALLPLMLASCGGNFGVQPVVESTPTAYPVTFKSKD
                     VPTSPPAEPSVETTPVNRPAVGAAMRLLRRNTAFHREDGTAIPDSKQAEEKLSFKEGD
                     VLFLYGSKGNKLQQLKSEIHKRDSDVEIRTSEKENKKYNYKFVDAGYVYTKNG"
BASE COUNT         1299 a         1426 c         1697 g         1378 t
ORIGIN      
        1 cgaagggaac gattccctaa ggtgatgaag cgccgggcga accggttccg taccattcgt
       61 actgcctgcg gcccgccgcc ttgtcctgat ttttgttaat ccgctatggt ttaaagcttt
      121 gtttcttaag tccgcagagt atgccatggt taaaccttca acgtcgagtg ttgtactatt
      181 ttgtttttaa ttgaatatag aattgagggc ggaacagtcg gtgcaggcac cgtttgaaac
      241 caggcaaatc agctatgcct ggcgggaatc gggcagccct gaagaaaccg tattgaccgc
      301 acaaaccgcc gattccccgg atttacccgt acaggcagta ttgaacctaa cgcaccgttc
      361 ccataacggg aaacccctaa ttctcccctg acttcagacg gcataaagtc ggcatgccgt
      421 ctggaaacca aaaatctaaa aaggaacaac catgcaaacc ctgaccatca tccgacccga
      481 cgatatgcac ctgcacctgc gcgacggcga cgcgctcaaa gccgttgccc cttataccgc
      541 ccgccagatg gggcgcgccg tcatcatgcc caacctcaaa ccgccagtcg tcagcgtagc
      601 cgacgcgctt gcctacaaag cgcgcattat ggcggcgttg cccgaaggca gcgcgtttga
      661 gccgctgatg acgctttacc tgaccgacca agccacgccc gaactcgtgc gcgaagccaa
      721 agccgccggc atcgtcgcct tcaaactcta ccccgcaggc gcgaccacca attccgattc
      781 cggcgtaacc gacctgttca aactcatccc cgttttggaa gaaatggcaa aacagggcat
      841 cctgttcctc gttcacggcg aagtaaccga ccccgaaatc gacattttcg accgcgaagc
      901 cgcctttatc gggcgcgtga tgaaacccgt tttggcgcaa gtgccgaatc tcaaagtcgt
      961 gttcgaacac atcaccaccg ccgaagccgc ccgcctcgtt ttggaagcgg gcgacaacgt
     1021 tgccgcctcc gtgaccccgc aacacctcct gctcaaccgc aacgacctct tggtcggcgg
     1081 tgtgcgcccc catcatttct gcctgcccgt cctcaagcgc gaaacccacc gtcaggcatt
     1141 ggtcgccgcc gttaccggcg agaaggcgca caaattcttc ctcggcaccg actccgcgcc
     1201 gcacgccaaa tccgccaaag aaaacgcctg cggctgcgcc ggtatgttca gcgcgatgac
     1261 cgccgtcgaa ctttacgccg aagtgtttga aaaagcaggc gcgttggaca aactcgaagc
     1321 cttcgcctcg aaaaacggcg caaggttcta cggcattccg gaaaacgccg acacgattac
     1381 ccttgtcaaa caaagccaaa ccgttcccgc aagcgtccct tacggcgacg gcgaacttgt
     1441 cccgatgcgc gcgggcggcg aaatcggctg gacggtgcag tattgattga acggtcaagc
     1501 aaaatgccgt ctgaaaggtt ttcagacggc atttgtgtat tttccgattc ggattttccg
     1561 agtatcaacg gcgtttgggc tcgtctgggc ggatttgggc ggcgagtttg tcgaggatgc
     1621 cgttgacgaa tttgtgcccg tccgtgccgc cgaaggtttt ggtaacttcg atggcttcgt
     1681 tgataatgac ggggtagggc gtttcgggca tagcggaaag ctcgtggcag gcggtcagca
     1741 aaacggcgcg ttcgatgggg ttgaggtctt tttcgtccct gtcgagcagc gggcggattt
     1801 tttggatgta gtccgctgcg ttggtttgtg tgccgaagaa gagtttgttg aacaattctt
     1861 cgtccgcttt ggcaaaatcg gacatttcgc ggatgttttt agcaatctcg ggtgctgcgg
     1921 tgcggttgat aagggattgg taaacggctt gtacggcaag ctcgcgggaa cggcggcggg
     1981 ctgttttcat gatttttcct tgaaacggtt gggcggcacg gtatgccgtc tgaaacggaa
     2041 agggcgcatc ggtgtacgcc ctgtttgtta ttcttcgtct tcaaactgtt cttcgagcag
     2101 aaggttgacg aggttggcgc attctacggc gactttggcg gcatccgagg ctttttctcc
     2161 aatccgttca attgcctgcg cgtcgttttc ggtggtcagg acggcgttgg caatcgggat
     2221 gttgtagtcg agtgcgacgc ggccgatccc tgcgccggat tcgttggcaa ccagctcgaa
     2281 atggtaggtt tcgccacgga tgacgacgcc gatggcaatc agtgcgtcaa atttttcgga
     2341 agaggcaaag ttcatcagcg cgatggggat ttcaagcgcg ccgggtacgg tggcgacggt
     2401 gatgttttcg tctgctacgc ccaattcttg gagggtgcgg cagcagactt tgagcatttg
     2461 gctgccgatt tcgttggtga agcgtgcctg tacgatgccg atgcggaggt gtttgccgtc
     2521 gaggttgggg gcgatggtgt tcattgggtg tcctttggta ttcggggttt cggaatgccg
     2581 tctgaaggtt tcagtcttgc ggctgccagt cggcaacggt ttggaatgtg ccgtcttcgg
     2641 caagctccca cgcgctgcct tcgggttggg aaagcagtgc ggcggtttca gggttggttt
     2701 tggtgatgtc ggcgaggctg acgatgctga agttgtcggg gtcgtcggtg tattcgtcgg
     2761 tttcgtcgcc gctgaagaaa cgccagccgc tgtcgttttc aaaaacgggg gcttcgcggt
     2821 aaaggaagcc gacgggccgg ttttgtttgg cgacggtgtt ggtggcgatg cagcggtcga
     2881 gtgccgagga aagtgcttgt gcaaatgcgt tcattgcggg aatacgttgg gggggggaac
     2941 ttgcggattt taccacgatt cccgcgttgt cggcagacgg cggcggtttg gtggtacaat
     3001 gtgcgccgtt tgcagcctta aggtgtttct gtatttttgg agtatggaaa cgcattcggg
     3061 ctgttttttg cggaagacgg taatgaaaga cgatgttttg aaacggcagg cacatactgc
     3121 gatacagaaa aagttaggct acgcgttccg cgatatgtcg cttttgcggc gggctttgac
     3181 ccacaggagc catcatgcga agcacaacga acggttcgag tttgtcggcg attcgatttt
     3241 gaattatacg gtggcgcgga tgctgtttga cgcgtttccg aagttgaccg agggcgagtt
     3301 gtcgcggttg agggcgagtc tggtcaatga gggcgtgctg gcggaaatgg cggcggaaat
     3361 gaatgtcggc gacggtctgt atttgggggc gggcgagttg aagagcggcg gcttcagacg
     3421 gccttcgata ctggcggacg cgatggaggc gatgtttgcc gccgtcagct tcgatgccga
     3481 tttcaacacg gcggaaaagg tggtgcgcca tttgtttgca gaacgcgtcc ggcgcgccga
     3541 tttccaaaat caggcaaaag acggcaaaac tgctttgcag gaggcgttgc aggcgcgccg
     3601 tttcgccttg ccgaaatacc gcatcgaaga gcaaatcggc catgccgacg acagtatgtt
     3661 tgtcatttcc tgcgatttgg gcgaactggg tttcgtgtgc cgtgccaaag ggacgagccg
     3721 caaggcggcg gaacaggagg cggcgaaaga ggctttgaaa tggctggaag agaagctgcc
     3781 gctgaagaag aaaaagaaat gaggcggcgc gtgaatatgc cgtctgaaat gtggatatga
     3841 aagcgaatat ggatattgaa accttccttg caggggaacg cgccgccggc ggataccgtt
     3901 gcggcttcgt ggcgattgtc ggtcgtccga acgtgggcaa atcaacgctg atgaaccatc
     3961 tcatcggtca gaaaatcagt attaccagca aaaaggcgca gacgacgcgc aaccgcgtaa
     4021 cggggattta taccgacgat accgcgcagt tcgtgtttgt cgatacgccg ggctttcaaa
     4081 ccgaccaccg caacgcgctc aacgacaggc tgaatcaaaa tgttaccgag gcgctcggcg
     4141 gtgtggatgt ggtggttttc gtcgtggagg cgatgcgcct taccgatgcc gaccgcgtcg
     4201 tgttgaaaca actgcccaag cacacgccgg tcattttagt gatcaacaaa atcgacaagg
     4261 acaaggcgaa agaccgttac gcgctggagg cgtttgttgc ccaagtgcgc gccgaatttg
     4321 aatttgcggc ggcggaggcg gtcagtgcga aacacggttt gcggattgcc aacctgttgg
     4381 agctgctcaa gccgtatctg cccgaaagcg taccgatgta tcccgaagac atggttacgg
     4441 acaaatcggc gcgttttttg gcgatggaaa tcgtgcgtga aaaactcttc cgctatttgg
     4501 gcgaggagct gccttatgcg atgaacgtcg aagtggagca gtttgaagag ggagacggtt
     4561 tgaaccgcat ctacatcgcc gttttggtcg acaaagaaag ccaaaaggcg attttgatcg
     4621 gtaaaggcgg ggagcgtttg aaaaaaattt ccaccgaagc gcggctggat atggaaaaac
     4681 tgtttgataa caaagtattt ttgaaggtct gggtcaaagt caaatccggt tgggcagacg
     4741 acattcgctt cctgcgcgag ctgggtttgt agtttttctt gctgaacttt acgcaaatgc
     4801 cgtctgaaca ggtttcggac ggcattttgt ttcaatcggg aatatctttg ttaaaaacgg
     4861 gttgatatta tctgtgcata ttatagcgga ttaacaaaaa ccggtacggc gttgccccgc
     4921 cccggctcaa agggaacggt tccctaaggc gcccaagcac cgggcgaacc ggttccgtcc
     4981 catttgtact gtctgaggct tcgccgcttt gtcctgattt tgttaatcca ctatatgaat
     5041 cgtcattccc gcgcaggcgg gaatccagac attcaatgct aaggcaattt atcgggaatg
     5101 actgaaactc aaaaaaccgg attcccactt tcgtgggaat gacgggatgt atagtgaatt
     5161 aaatttaaac cagtacggcg ttgcctcgcc ttttcgtact atctgtactg tctgcggctc
     5221 gtcgccttgt cctgattttt gttaatccac tataattcac tataaaaact ggttgatatt
     5281 atctgcgcat attaatataa tgataattat tattaattaa atagggggga tagggatgcg
     5341 taaactgaat tattacggta ttgccttgct gccgttgatg ctggcatcct gcggcggcaa
     5401 tttcggcgtg cagcctgttg tcgaatcaac gccgaccgcg taccccgtca ctttcaagtc
     5461 taaggacgtt cccacttccc cccctgccga gccttcggta gaaaccacgc cggtcaaccg
     5521 gcccgccgtc ggtgcggcaa tgcggttgtt gaggcggaat actgcttttc atcgtgaaga
     5581 tggcacggca attcccgata gcaaacaagc agaagaaaag ctgtcgttta aagaaggtga
     5641 tgttctgttt ttatacggtt caaaaggaaa taaacttcaa caacttaaaa gcgaaattca
     5701 taaacgtgat tccgatgtag aaattaggac gtcagaaaag gaaaataaaa aatataatta
     5761 taaatttgtg gatgccggtt atgtatatac taaaaacgga
//
LOCUS       DAXFZR010000108         5717 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000108 DAXFZR010000000
VERSION     DAXFZR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.109"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..213)
                     /locus_tag="ACFJAS_001961"
     CDS             complement(<1..213)
                     /locus_tag="ACFJAS_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983123.1"
                     /translation="MQTVATKPTAKQMLAAKRAAKESTRQERAVKRAGTVRNVDRNRL
                     SARSKAQKENIARMLSGAKVSEDEALT"
     gene            complement(324..539)
                     /locus_tag="ACFJAS_001962"
     CDS             complement(324..539)
                     /locus_tag="ACFJAS_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983124.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
     gene            complement(591..1082)
                     /locus_tag="ACFJAS_001963"
     CDS             complement(591..1082)
                     /locus_tag="ACFJAS_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFC3983125.1"
                     /translation="MTALTLYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQEITEKMLEGHIRRMTGKLKAAKARNQSLKDYLARNMQAAGITEIKADDGT
                     FKASFRKSEAVVILDEAQIPAEFMREAVKTEPDKTAIKKAIESGRQVAGAKIEGRKNL
                     QIR"
     gene            complement(1079..1261)
                     /locus_tag="ACFJAS_001964"
     CDS             complement(1079..1261)
                     /locus_tag="ACFJAS_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983126.1"
                     /translation="MANIDLTQWDGKTIGAAANPEQGYINITIGSDDLFINIEQAYAI
                     HAALGEAVAEYEGGAQ"
     gene            complement(1401..2087)
                     /locus_tag="ACFJAS_001965"
     CDS             complement(1401..2087)
                     /locus_tag="ACFJAS_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication initiation protein, NGO0469
                     family"
                     /protein_id="HFC3983127.1"
                     /translation="MSLILSVKDESNFKPCPAGSHHATCIRIIDLGTQLVEYQNEQKR
                     QHKILVQWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQLATDLKSWRGRDFTPEE
                     RDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNKMKSYTPKHPDNAVFAFDLS
                     DPDWANYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQAQAAEGRPEHPQGNAAPA
                     EDIEDDIPFN"
     gene            complement(2156..2317)
                     /locus_tag="ACFJAS_001966"
     CDS             complement(2156..2317)
                     /locus_tag="ACFJAS_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983128.1"
                     /translation="MSFHPETAYTGGGETEPYGPSPEEIKYRQSPETAETRRMTEKQA
                     EGHIKSIIR"
     gene            complement(2314..2592)
                     /locus_tag="ACFJAS_001967"
     CDS             complement(2314..2592)
                     /locus_tag="ACFJAS_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO1622 family putative holin"
                     /protein_id="HFC3983129.1"
                     /translation="MKTRNIAFKFAVLAAVLAAGYAFGFAKGGGSRTAKGKPAGIAAM
                     RMALAQKQAEVAELSAEIWLEERHLNADEEEAGCRRVHGDAEVPGGKE"
     gene            complement(2745..3077)
                     /locus_tag="ACFJAS_001968"
     CDS             complement(2745..3077)
                     /locus_tag="ACFJAS_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983130.1"
                     /translation="MKYYGTAAYGSPDWGMERYYAREDMRQALDGWEAENRILHESGL
                     IEIAKKSAREFVRDADGEPYAQEDWETYLTEDASRIGKDTEAAMNYAIDEREWFALAE
                     NIGRLANS"
     gene            complement(3216..3491)
                     /locus_tag="ACFJAS_001969"
     CDS             complement(3216..3491)
                     /locus_tag="ACFJAS_001969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983131.1"
                     /translation="MSYLDQPLKHGYGNGNGSGNGSGYGNGYGKGYGNGYGNGYGNGN
                     GSGYGNGKGDGNGSGNGDGDGNGNGSGNGSGYGNGYGNGYSNGSGNG"
     gene            complement(3488..3964)
                     /locus_tag="ACFJAS_001970"
     CDS             complement(3488..3964)
                     /locus_tag="ACFJAS_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983132.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSAGVWFGVLKQKAGNEVILTKARRMYSWW
                     AKESISLSGVARHGIRQDGSQICGELDSVWLEAIEIIPVTGGAAESIRTALEVAQS"
     gene            complement(3997..4197)
                     /locus_tag="ACFJAS_001971"
     CDS             complement(3997..4197)
                     /locus_tag="ACFJAS_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983133.1"
                     /translation="MNELTGRINRFGARAKDEQSLLLKVGEICRDAAATWTTRKSESI
                     NHTAFTFTVKKDGLKEKVMIVL"
     gene            complement(4395..4808)
                     /locus_tag="ACFJAS_001972"
     CDS             complement(4395..4808)
                     /locus_tag="ACFJAS_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983134.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(4805..5266)
                     /locus_tag="ACFJAS_001973"
     CDS             complement(4805..5266)
                     /locus_tag="ACFJAS_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3983135.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(5283..>5717)
                     /locus_tag="ACFJAS_001974"
     CDS             complement(5283..>5717)
                     /locus_tag="ACFJAS_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983136.1"
                     /translation="ELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGLY
                     KNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQPN
                     GTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
BASE COUNT         1043 a         1504 c         1416 g         1754 t
ORIGIN      
        1 cgtcagggct tcgtcttccg atacctttgc gcccgacaac atccgggcga tgttttcttt
       61 ttgcgctttt gaccgggcgg acagccggtt ccggtcaacg tttctgaccg tcccggcgcg
      121 tttgacggcg cgttcctgcc gcgttgattc cttcgccgcg cgtttggcgg caagcatctg
      181 ttttgccgtc ggttttgttg ctactgtttg cattttgttt tctcgatttt ttgatgccgt
      241 tctctcaatg cccaatcata aagctgtatc tctcacgggg tcgccgaatt taaattgata
      301 gttcatgtct tgttccatta atatcaaacg caatcttcaa acacctcaat tacatttttt
      361 aaatcgctaa taccataatt tattacatcc tttagaaatt ccaaagaggt atccgcttcg
      421 tctgctttat ccctaatttc gtctatataa ccctctaacg attcaggctc ttttaatgct
      481 tctttgcata agttatctat tacccttaat gcgtttttta catcttccaa atagctcatt
      541 ttttgctcct taactcaaaa tgggatgctg tcgtcaacat cttctacggt ttatctaatc
      601 tgcaaattct tccgcccttc aatcttcgcg cctgctactt gccgaccgct ttcaatcgct
      661 tttttgatgg cggttttgtc cggttcggtt ttgacggcct cacgcataaa ttcggcgggg
      721 atttgtgctt cgtctaagat cacgacggct tcggatttgc ggaacgaggc tttaaaagtg
      781 ccgtcgtccg ctttgatttc ggtaatgccc gccgcctgca tattgcgcgc caagtagtct
      841 ttcaggcttt gattccgcgc ttttgccgcc ttgagcttcc cggtcatccg cctgatgtgc
      901 ccttcaagca ttttttccgt gatttcttgg tttttaatat aagcgataac ggattgcgct
      961 ttgacctcga actgcccgat aacggcttcc agcgtgtctt cgcgctcggt ttcgctgtcg
     1021 aagtagtaat caagcgccgc ctgtacgtct gccgcgcacc ggtagagtgt gagggcggtc
     1081 attgtgcccc tccctcatat tcggcaaccg cttcgccaag cgcggcgtgt atggcgtatg
     1141 cctgttcgat gttgatgaat aggtcgtcgc tgccgatggt gatgttgatg tatccctgtt
     1201 ctggattggc ggcggcgcca atggttttcc cgtcccattg ggtcaggtcg atgtttgcca
     1261 tttttttgtt tcctttctct gttgccgtcc gaagcagttg caaactaaaa atcgactact
     1321 gcttcagagt gcggggcctg cccgcagggc gcggcgtttg tttgcggttt tccgtccggt
     1381 tttacgccct gacggcgggc ttaattaaaa gggatgtcgt cctcgatgtc ttcggcaggc
     1441 gcggcattgc cttgggggtg ttccggccgc ccttccgccg cttgggcctg tttctgcggc
     1501 ggcgccggcg gttggcggcc gtttacggct tcggcatatt ccgggctttt ggcaatctgc
     1561 tccctcagtt tctcgttcag gaggccgtaa ttcgcccaat cggggtctga caggtcgaag
     1621 gcaaaaacgg cgttgtccgg atgtttcggg gtgtagctct tcatcttgtt gctgatggcg
     1681 gaaatgttgg cataggtggt tttgccgtcg ctgctttctt ggtgggcgat actcaacagg
     1741 cagggcttgc ccaaaatatt gcgcaaatcg aagttgtcgc gttcttccgg tgtaaagtcc
     1801 cttccgcgcc agcttttgag gtctgttgcc agttggcttt tgctgtgcag gctggcggtg
     1861 taccggcggc tgatgaggta gggcctgccg tccggcatca gcatttccgg atcgccttca
     1921 gggtcgattt cccactgcac taaaatcttg tgctgccgct tttgttcgtt ttggtactcg
     1981 acgagctgcg tacccaaatc gatgatgcgg atgcaggtgg cgtgatggct gcctgccggg
     2041 catggtttga aattgctttc gtctttcaca cttaaaatca atgacatttt cggtctcctg
     2101 ttaaaggtcg tttcgtctat cggtctcgcg ctgttttatg ccttgcgcgg cggcgttacc
     2161 tgataatgct tttaatgtgg ccttctgcct gtttttcggt catccgccgt gtttcggcgg
     2221 tttccgggct ttgccggtat ttgatttctt cggggcttgg tccgtacggc tctgtttctc
     2281 cgccgccggt gtaagcggtt tcgggatgga agctcattct ttaccccccg gcacttccgc
     2341 atcgccgtgc acccgccggc aaccggcttc ttcttcatcg gcattcaggt gccgctcttc
     2401 cagccagatc tcggcgctca attccgcaac ttccgcctgc ttttgagcca acgccatgcg
     2461 cattgccgca ataccggcgg gttttccctt tgccgtacgg cttccgcctc ccttggcgaa
     2521 tccgaaggca tagcccgccg ccaataccgc cgccaatacc gcgaacttaa acgcaatatt
     2581 ccttgtcttc atttctattt ccttaattta aaaggtttta attgcgcacc gcgtccgcta
     2641 aggatggtac ggaccgtgcg ccgtcggggt tatttgcggc taaaatctac aaaaaccgcc
     2701 gccgcgccca ctccccggct gacggcgcgg cattcctatg cccgctatga atttgccagc
     2761 ctgccgatgt tctccgccag cgcgaaccat tcccgctcgt ctatggcgta gttcatcgcg
     2821 gcttcggtat ctttaccgat acgggaagca tcttccgtaa ggtacgtttc ccaatcttct
     2881 tgggcgtaag gttcgccgtc cgcatcgcgg acaaactccc gcgccgattt tttggcaatc
     2941 tcaatcaacc cggattcgtg caggattcgg ttttccgcct cccaaccgtc caaagcctgc
     3001 cgcatatcct cccgcgcgta atatctttcc atcccccaat cggggctgcc gtaagccgcc
     3061 gtgccgtaat atttcatcgc tttatcctta agtttgagga aaccgcccgc agcattcacc
     3121 gtttcgccgt gccgttgccc cgctttgaag tgctatactt catcgctttg tgctatcccc
     3181 ggcttggcgg atatagcttc gggcggtttt aaggtttagc cgttgccgct gccgttgctg
     3241 tagccgttgc cgtagccgtt gccatagccg ctaccgttgc cgctaccgtt gccgttgccg
     3301 tcgccgtcgc cgttgccgct gccgttgccg tcgcccttgc cgttgccgta gccgctgccg
     3361 ttgccgttgc cgtagccgtt gccgtagccg ttgccgtagc ccttgccgta gccgttgcca
     3421 tagccgctac cgttgccgct accgttgccg ttgccgtagc cgtgcttcaa tggttgatct
     3481 agataactca tgactgggcg acctccagcg cggtgcggat tgattcagcc gcgccgcctg
     3541 ttactgggat aatctcaatc gcctcgagcc atacggaatc aagctcgccg caaatttggc
     3601 tgccgtcttg cctgatgccg tgtcgtgcga cacctgacag gctgattgat tcctttgccc
     3661 accagctgta cattcggcgc gcttttgtca gaatcacttc attgcctgct ttttgtttca
     3721 acacaccaaa ccaaacgcct gccgaataag tgcggatgat gacttccttg ccgatggcaa
     3781 agtcgttgat acctttttgc tcggcaactg ttaccggcgg ttgcggctca tgttgcggtt
     3841 cgtcaaattc ggttgaaatg tcggcgcgtt ttacacccat tgccgcttcg aaatcggcag
     3901 caatgcctgc aaagactttt ataaggtctg acaaactttt cacttcaaat ttatttgctt
     3961 ccatttttgt ttcctttcgg ggtggggttg gtttctttac aaaacaatca ttaccttctc
     4021 ttttaagccg tcttttttaa ccgtaaaagt aaaagcggtg tgattgatgc tttcgctttt
     4081 tctagtggtc catgtcgctg ctgcgtcgcg gcagatttca ccaactttca ataaaaggct
     4141 ttgctcgtcc tttgccctcg cgccaaaccg atttattctg ccggttaatt cgttcatccc
     4201 gtttccttca agttgttgtt tgtttcgatg ggtgtattat aactattacc tatatttaat
     4261 caataggcaa tagttatatt ttcatccata tatacttatt gaattgattt ttagaagaaa
     4321 aaagtttgaa aaaaaaccgc ccgaatggcg gtgtatcatg aaaaagaaaa ccgccctagg
     4381 gcggttcggt attgttatga gtggtaagga agccaagaaa aagacaaagg acggctctcg
     4441 gagagaatat ggttgtgtgt tgtcattctt acataaatgc ggtcgttgtc tgaaacgggt
     4501 agtccgctga ttgtttggtg tagcatgaat gacacgtttt cttctgtatc actgccgtca
     4561 tatgatggaa tctcaagtgc agagattaca tcatcgttgc ggacgatttc cagaaaaatt
     4621 tgaccggttt tcatagtctg tatatccatg ccttcggcgg aaaatgaaac agacaggcac
     4681 aggcggccta gaatggcctg cggtgtcgga atatggcata tatcggggaa tatacctatc
     4741 aggctgtatt tgtcattttc cgcatggcgg ataatttcat cgcaatagtg gacatttaag
     4801 tgaatcatgc ctgctccata tattcgtacc gccgttcgaa cgctgcgcga acttcaagcg
     4861 gggaaacgcc taatgcgttt gccaattttt gtacagtctt atcttgcagg gattgtttgc
     4921 tgttttcgat gcgggacaga tagggttgag gcaatcctgc ggcggttgcc aattcggact
     4981 gggtaaaccc ctttttcatc cttaagctga caaatgtttc cccgcctgct ttgagggcta
     5041 ttttatcggc aattcttgct gccgccctgt ccatcgctgc tttgcggcgc ggatttttcc
     5101 gcacatgctc aatatgttct gcggcagggg tagtgttagg gcttatcggt ttgacgcatt
     5161 taatctgccc gtttatccgc aggcatttta tgtgggtgac ggggatggcg gatgtaggtg
     5221 ttgatactgt gcaggcggcc acggcaacag tgagtgttgc acccattttg aaccatgtgc
     5281 cgttagttgg agtacagttc ttcatagtct ttgataatcc gttttgtgat tgggtgttct
     5341 gcctgatagt caaattcgtc tgtcttcttg tttacaacag ccaatatatc tatgcggcgg
     5401 atatacgtgc cgtttggctg tttttcatgg catggtgcgt aaattattct gtatccggcg
     5461 gcttcttcgc tgtcaaatcg gacgcgtaaa actttgatgt cttttcccca taatgacaat
     5521 atgggtttca cttccaagtc aatcgggcct atgggttcgc catattccct gaagtatctg
     5581 tttttgtata agccgtcaaa aatagcagag tccgcctgaa tcatcgcaat gacattctcg
     5641 agataaccga ccgcttcttc gtctttttca aagaggcggt ctaaatcctg ctcggcgtta
     5701 aagtggacgg tcagctc
//
LOCUS       DAXFZR010000109         5417 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000109 DAXFZR010000000
VERSION     DAXFZR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.110"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            317..811
                     /locus_tag="ACFJAS_001975"
     CDS             317..811
                     /locus_tag="ACFJAS_001975"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HFC3983137.1"
                     /translation="MQPITGNFDLAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFA
                     NFAFIENESRKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLF
                     MTQLAIKNGFQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQ
                     TTIF"
     gene            824..1210
                     /gene="iscU"
                     /locus_tag="ACFJAS_001976"
     CDS             824..1210
                     /gene="iscU"
                     /locus_tag="ACFJAS_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC3983138.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            1297..1617
                     /gene="iscA"
                     /locus_tag="ACFJAS_001977"
     CDS             1297..1617
                     /gene="iscA"
                     /locus_tag="ACFJAS_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC3983139.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(1577..1798)
                     /locus_tag="ACFJAS_001978"
     CDS             complement(1577..1798)
                     /locus_tag="ACFJAS_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC3983140.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            1880..2380
                     /gene="hscB"
                     /locus_tag="ACFJAS_001979"
     CDS             1880..2380
                     /gene="hscB"
                     /locus_tag="ACFJAS_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC3983141.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            2478..5228
                     /gene="gyrA"
                     /locus_tag="ACFJAS_001980"
     CDS             2478..5228
                     /gene="gyrA"
                     /locus_tag="ACFJAS_001980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC3983142.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT         1554 a         1472 c         1265 g         1126 t
ORIGIN      
        1 gtttgaaata gttaactatt gaagatcatt ggacaaaaga tgttgatgat atcactggtt
       61 catggaaaat ggagaattta gaaattagcc gacaaactca atatgcaatg caacttctaa
      121 agattggtaa aacaaatatt gagaaagaaa taagaaaaat taaacaaagg ttctaaaaaa
      181 atgagtaaat acggtggtga agacgtaatc tatatgaatg atggtgtatt tagaaataaa
      241 ttagccattc aagatccact taaatttaaa aaaggtcgaa agagacattt ctgctatttc
      301 cgcccaaaat ctgcttttgc aacccattac aggcaatttc gatttggcgc atttacagac
      361 cattcatcgc gaactgtttg gcaatgttta tgattgggca ggaaaaatcc ggagggttga
      421 tatttctaaa ggtaataccc gatttgccaa ttttgctttt atcgaaaacg aaagccgtaa
      481 acttttggag aagctgaaaa acgaaaacta tttgcgggtg ttggataaag ataaattcgc
      541 cgagcgtgca gcctattatt tggacgagtt gaacgtccta catccttttc gtgaaggaaa
      601 cggcagaaca ttgcgcctgt ttatgacaca attggcaata aaaaacggtt ttcagataca
      661 ttggcaaaat atctctgccg aacaaatgat tcaagcctgt attcaggcat accatgccga
      721 tagcagctta ttggcacgcc taattattag acaatttaga acaatcgtct ttttcagacg
      781 actaaaacat caacaaacca cgatatttta aggaaaccac atcatggcat acagcgataa
      841 agtaatcgac cactacgaaa atccccgcaa cgtcggcact ttcgacaaaa acgacgagtc
      901 cgtcggcacc ggcatggtcg gcgcgccggc ctgcggcgac gtgatgcgcc tgcaaatcaa
      961 agtgaacgat gaaggcatca tcgaagatgc gaaattcaaa acctacggct gcggttccgc
     1021 catcgcttcg tccagcctga ttaccgagtg ggtcaaaggc aaaagtctgg atgacgcgct
     1081 ggcaatcaaa aacagcgaaa tcgccgaaga actggaattg ccgccggtaa aaatccactg
     1141 ctccatcttg gctgaagatg cggtaaaagc ggccgttgcc gactaccgca aacgtcagga
     1201 aaacagataa agcccttcag acggcatcgt cccacaatgc cgtccgaacc acccgccgct
     1261 tcggatccgt ccggggcggt acaacaagga agaaatatga ttacccttac cgagaatgcc
     1321 gcaaaacaca tcaatgacta tctcgccaaa cgcggcaaag gcttgggcgt acgcttgggt
     1381 gtaaaaacca gcggctgctc ggggatggcg tacaaccttg aatttgtcga tgaagccaac
     1441 ggcgacgacc tgattttcga aggacacggc gcgcgcattt atatcgaccc gaaaagcttg
     1501 gtttatctgg acggcacaca agtcgattac accaaagaag atttgcagga aggtttcaaa
     1561 tttgaaaacc ccaatgtcaa agactcctgc ggctgcggcg agagcttcca cgtttaaggc
     1621 ataaaaacgg cgggacagta tcaaaaccgt cccgccgttt tttcgcttcc tgcctgttgt
     1681 agctgccttt gcctttcctt ttccgttcca ccttgtgccg gaacaaatcg gattttacta
     1741 aggcttttaa agcattgtcg cgtattttgc ctttattgtg ctgcgctttg ccgcccatat
     1801 tcagtccttt cgtttaagaa gccgcagatt ataaggcaaa aacagttttc tgccaaaatc
     1861 ttacatttat catcctacta tgtcccaata tttcaccctc ttccggattg aacccgcttt
     1921 cgatatcggc accgaaaact tggaacaaac ctaccgcgcc ttggccgccc gtttccatcc
     1981 cgacaaattc gcttccgctt ccgctttcga gcagaaacag gcggttatga tgtcttccac
     2041 catcaacgat gcctaccgca ccttgaaaaa tcccatcgac cgcgccgcgt acctgctgaa
     2101 aacatcgggt atcgatgccg acgcgccgga acatacctct ttcgcccccg acttcctcat
     2161 gcagcaaatg gaatggcgcg aaacgctgat ggaggcacgg gcaggcaaca accttgaatc
     2221 cttgaaaaat ctcgataacg aaatccgcgc cgaacaagaa aaactgttct gcggtctgaa
     2281 acagtcattc gcgcgccaag actgcgacac cgccgcacaa caagtccgcc aaggcaggtt
     2341 tctcgacaaa ctccgccacg aaatttcctc cgcattataa tccgcaccgt gtttcagacg
     2401 gcataaccgc cgcaccgttt catgtcgaaa tatgctaaaa taagcaacaa ttttttgcca
     2461 tacgaaacat tgaaaccatg accgacgcaa ccatccgcca cgaccacaaa ttcgccctcg
     2521 aaaccctgcc cgtcagcctt gaagacgaaa tgcgcaaaag ctatctcgac tacgccatga
     2581 gcgtcattgt cgggcgcgcg ctgccggacg ttcgcgacgg cctaaagccg gtgcaccggc
     2641 gcgtactgta cgcgatgcac gagctgaaaa ataactggaa tgccgcctac aaaaaatcgg
     2701 cgcgcatcgt cggcgacgtc atcggtaaat accaccccca cggcgattcc gcagtttacg
     2761 acaccatcgt ccgtatggcg caaaatttcg ctatgcgtta tgtgctgata gacggacagg
     2821 gcaacttcgg atcggtggac gggcttgccg ccgcagccat gcgctatacc gaaatccgca
     2881 tggcgaaaat ctcacatgaa atgctggcag acattgagga agaaaccgtt aatttcggcc
     2941 cgaactacga cggtagcgaa cacgagccgc ttgtactgcc gacccgtttc cccacactgc
     3001 tcgtcaacgg ctcgtccggt atcgccgtcg gtatggcgac caacatcccg ccgcacaacc
     3061 tcaccgacac catcaacgcc tgtctgcgtc ttttggacga acccaaaacc gaaatcgacg
     3121 aactgatcga cattatccaa gcccccgact tcccgaccgg ggcaaccatc tacggcttgg
     3181 gcggcgtgcg cgaaggctat aaaacaggcc gcggccgcgt cgttatgcgc ggtaagaccc
     3241 atatcgaacc cataggcaaa aacggcgaac gcgaagccat cgttatcgac gaaatcccct
     3301 atcaggtcaa caaagccaag ttggtcgaga aaatcggcga tttggttcgg gaaaaaacgc
     3361 tggaaggcat ttccgagctc cgcgacgaat ccgacaaatc cgggatgcgc gtcgttatcg
     3421 agctgaaacg caacgaaaat gccgaagtcg tcttaaacca actctacaaa ctgactccgc
     3481 tgcaagacag tttcggcatc aatatggttg ttttggtcga cggacaaccg cgcctgttaa
     3541 acctgaaaca gattctctcc gaattcctgc gccaccgccg cgaagtcgtt acccgacgta
     3601 cgcttttccg gctgaagaag gcacgccatg aagggcatat cgccgaaggc aaagccgtcg
     3661 cactgtccaa tatcgatgaa atcatcaagc tcatcaaaga atcgcccaac gcggccgagg
     3721 ccaaagaaaa actgcttgcg cgcccttggc gcagcagcct cgttgaagaa atgctgacgc
     3781 gttccggtct ggatttggaa atgatgcgtc cggaaggatt ggctgcaaac attggtctga
     3841 aaaaacaagg ttattacctg agcgagattc aggcagatgc tattttacgc atgagcctgc
     3901 gaaacctgac cggcctcgat cagaaagaaa ttatcgaaag ctacaaaaac ctgatgggta
     3961 aaatcatcga ctttgtggat atcctctcca aacccgaacg cattacccaa atcatccgtg
     4021 acgaactgga agaaatcaaa accaactatg gcgacgaacg ccgcagcgaa atcaacccgt
     4081 tcggcggcga cattgccgat gaagacctga ttccgcaacg cgaaatggtc gtgaccctga
     4141 cccacggcgg ctatataaaa acccagccga ccaccgacta tcaggctcag cgtcgcggcg
     4201 ggcgcggcaa acaggcggct gccaccaaag acgaagactt tatcgaaacc ctgtttgttg
     4261 ccaacacgca tgactatttg atgtgtttta ccaacctcgg caagtgccac tggattaagg
     4321 tttacaaact gcccgaaggc ggacgcaaca gccgcggccg tccgattaac aacgtcatcc
     4381 agctggaaga aggcgaaaaa gtcagcgcga ttctggcagt acgcgagttt cccgaagacc
     4441 aatacgtctt cttcgccacc gcgcagggaa tggtgaaaaa agtccaactt tccgccttta
     4501 aaaacgtccg cgcccaaggc attaaagcca tcgcactcaa agaaggcgac tacctcgtcg
     4561 gcgctgcgca aacaggcggt gcggacgaca ttatgttgtt ctccaacttg ggcaaagcca
     4621 tccgcttcaa cgaatactgg gaaaaatccg gcaacgacga agcggaagat gccgacatcg
     4681 aaaccgagat ttcagacgac ctcgaagacg aaaccgccga caacgaaaac accctgccaa
     4741 gcggcaaaaa cggcgtgcgt ccgtccggtc gcggcagcgg cggtttgcgc ggtatgcgcc
     4801 tgcctgccga cggcaaaatc gtcagcctga ttaccttcgc ccctgaaacc gaagaaagcg
     4861 gtttgcaagt tctaaccgcc accgccaacg gatacggaaa acgcaccccg attgccgatt
     4921 acagccgcaa aaacaaaggc gggcaaggca gtattgccat taacaccggc gagcgcaacg
     4981 gcgatttggt cgccgcaacc ttggtcggcg aaaccgacga tttgatgctg attaccagcg
     5041 gcggcgtgct tatccgtacc aaagtcgaac aaatccgcga aaccggccgc gccgcagcag
     5101 gcgtgaaact gattaacttg gacgaaggcg aaaccttggt atcgctggaa cgtgttgccg
     5161 aagacgaatc cgaactctcc ggcgcttctg taatttccaa tgtaaccgaa ccggaagccg
     5221 agaactgaaa atcatctccc gatgccgtct gaagattcag acggcattta tttttccctc
     5281 atccgtcatc cagcttctca caatatagcg gattaacaaa aatcaggata aggcgacgaa
     5341 gccgcagaca gtacaaatat agtggattaa atttaaacta gtacagcgtt gcctcgcctt
     5401 gccgtactat agtcaat
//
LOCUS       DAXFZR010000110         5412 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000110 DAXFZR010000000
VERSION     DAXFZR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.111"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..317
                     /locus_tag="ACFJAS_001981"
     CDS             <1..317
                     /locus_tag="ACFJAS_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983143.1"
                     /translation="DLSNCILTIRTSTKPSVEIEKWGLWLKDYDTVEIELRNSFIKGM
                     KCQNWSHNNRNICQVEIKNQEDGLKIIRFYDNNSNWLLELEVYGLVFQGCKTYMKEGY
                     ES"
     gene            307..1197
                     /gene="ubiA"
                     /locus_tag="ACFJAS_001982"
     CDS             307..1197
                     /gene="ubiA"
                     /locus_tag="ACFJAS_001982"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC3983144.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGFAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            1382..1831
                     /gene="ptsN"
                     /locus_tag="ACFJAS_001983"
     CDS             1382..1831
                     /gene="ptsN"
                     /locus_tag="ACFJAS_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC3983145.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEKAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            1835..2797
                     /gene="hprK"
                     /locus_tag="ACFJAS_001984"
     CDS             1835..2797
                     /gene="hprK"
                     /locus_tag="ACFJAS_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC3983146.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            2778..3632
                     /gene="rapZ"
                     /locus_tag="ACFJAS_001985"
     CDS             2778..3632
                     /gene="rapZ"
                     /locus_tag="ACFJAS_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC3983147.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            3713..4654
                     /gene="ylqF"
                     /locus_tag="ACFJAS_001986"
     CDS             3713..4654
                     /gene="ylqF"
                     /locus_tag="ACFJAS_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC3983148.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            4755..5357
                     /locus_tag="ACFJAS_001987"
     CDS             4755..5357
                     /locus_tag="ACFJAS_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC3983149.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
BASE COUNT         1339 a         1411 c         1425 g         1237 t
ORIGIN      
        1 ctgacttgtc taattgtatt ttaactatcc gaacaagtac aaagccttct gtagaaatcg
       61 aaaaatgggg gctgtggcta aaagattatg atacagttga aattgaatta agaaatagct
      121 ttattaaagg aatgaaatgt caaaattggt cgcataacaa tagaaatata tgccaagtag
      181 aaataaagaa ccaagaagat ggtctaaaaa taataagatt ttacgacaat aattcaaatt
      241 ggttattgga actagaagtt tatggattag ttttccaagg gtgtaagact tatatgaaag
      301 agggttatga atcttaaatc ccctttattt ttacgactgt ccgatcgttt ggacgtttat
      361 atcagactga tgagggcgga caagcccatt gggacgctgc ttttactgtg gccgacctac
      421 tgggcattgt ggctggcttc agacggcatt cccgatttgg cggtattggc ggcgtttaca
      481 atcggcacgt ttttaatgcg cagtgccggc tgcgtcatca acgactttgc cgaccgcgat
      541 tttgacggtg ctgtcgagcg caccaaaaac cgtccgttcg cacagggcag ggtcaagaaa
      601 aaagaagccc tgctgctgac ggcatttttg tgtctgcttg ccgcattgtg cctgattccg
      661 ctgaatcatc tgacatggct gatgagcctg cccgcgctgt tccttgcgct gacttacccg
      721 tttaccaaac gcttttttcc gattccccaa ttttatctgg gatttgcctt ttccttcggc
      781 atcccgatgg cgtttgccgc agtgggcaac agcgttcccg ttgaagcgtg gatactcttt
      841 gccgccaatg ttttatggac actggcttac gataccgtct atgcaatggc ggacaaagag
      901 gacgatttga aaatcggcat caaaacctcc gccgtcacgt tcgggcgtta cgacatcgcc
      961 gccgttatgc tgtgtcatgg cggctttacc ctgctgatgg cagtattggg tgcggttatc
     1021 ggtgcggcat gggcatattg gacggcaatc cccatcgtcc tgctgctgca ataccgccaa
     1081 tatgccgcaa tcaaaagccg cgtccggcaa atctgttttg aaacgttttt ggcaaacaac
     1141 agaatcggtt gggtgtggtt tgccgctatt tttgcccata cgtttttcgc gaaataaggc
     1201 agggcaatgc cgtctgaaga accgtaaact gctttggacg gcatttctat ctgtgccgaa
     1261 aagcgttaaa atatgttttt gaaacgctgt gttatgtcag cccgtaccgt atgcgggatt
     1321 gaggtttgcc ccggcagtcg gtacaatcct tctgttttgc gatgtctgaa aagagaagct
     1381 tatgagcctt atcggcgaaa ttttgccttt gtcccatatt gttttggata tggaggtggg
     1441 cagtaaaaaa aggctgtttg agaaagcggg cctgcttttg gaacgcgaat cctcattgtc
     1501 ccatgctgat gttttcgaat gtctttttgc ccgtgaaaaa ctcggttcga ccggtttggg
     1561 gcagggtgtc gccatccccc acggccgcca cgcgagcgtg aagcaggcga cgggcgcgtt
     1621 catccgtacg cgcgaacccg tcggattcga cgcgcctgac ggcaaaccgg tttcactggt
     1681 ttttatcttg ctggtgccgg aaaacgcaac gggcgagcat ttggaagtct tatccaaact
     1741 ggccggcaag ttttcccaaa aaagcatcag agaatcgctg atgacggttg cttctgcgga
     1801 agaagcccgc gccatcctga ccgaagaata aaatatgccc agtatctccg tccgccgcct
     1861 gtttgacgac aaccaataca agttgcaact tgcctgggcc gccggcaatt cgggtgcgga
     1921 caaccgtatc ggcgtagagg cggacaagcc cgtcctcgcc ctggtcggac acctgaattt
     1981 catccatccc aaccaaatcc aagtggtcgg tttggcggag tcggaatatc tgaaccgcct
     2041 cgaatcgggg gagacgggtt atcagttcgg agacctgttc gatatttcca tgtctttggt
     2101 cattgtggca aacgatttgc cggtttcccc ggggctgcgc gactactgcc acaaaaacga
     2161 catccccctg ttgacttcca aactcgaaag cccttatctg atggatgtgt tgcggattta
     2221 cctgcaacgc accttggcag catcgtccgt caaacacggc gtatttctcg atgtgtttga
     2281 aatcggcgtg ctgattaccg gacattctgg tttgggtaag agcgaattgg cattggaact
     2341 gatttcgcgc ggccatagcc tgattgccga cgatgcggtc gagctgttcc gcatcggtcc
     2401 ggaaacattg gaagggcgtt gttcgcctat gctgcgcgat tttttggaag tgcgcggctt
     2461 ggggatactc aatatccgcc atattttcgg cgaaacttcc atccgcccca aaaaaatcct
     2521 gcaactcatt atcaatttgg tcgaggcgga cgacgagtat atgaagcagc tcgaccggtt
     2581 gagcatccgc accgaaaccg aatccatcct caacgtcaac gtccgttcgg ttacgctgcc
     2641 cgtcgccgtc ggacgcaacc ttgccgtttt ggttgaggcg gcggtacgca attacatttt
     2701 gcagttgcgc ggcaaagaca gtacgcgcga atttttggaa cgccatcaga cgcaacttaa
     2761 agaaaacgaa cagaatcatg aaaatcgtcc tgattagcgg gctttccggc tcgggcaaat
     2821 ccgttgcact gcgccaaatg gaagatttgg gctatttttg cgtggacaac ctgcccttgg
     2881 aaatgctgcc gtcgctggtg tcgtaccata tcgaacgtgc ggacgaaacc gaattggcgg
     2941 tcagcgtcga tgtgcgttcc ggcatagaca ttgcccaagc gcgcgagcag attgcctatc
     3001 tgcgcggact cggacacagg gtggaagttt tgtttgtcga ggcggaagag gccgtgctgg
     3061 tccgccgctt ttccgaaacc cggcgcggac atcctttgag caatcaggat atgacgctgt
     3121 tggaaagcct gaaaaaagag cgcgaatggc tgttcccgct taaggaaatc gcctactgta
     3181 tcgacacttc caagatgaat gcccaacagc tccgccatgc agtccggcag tggctgaagg
     3241 ttgagcgtac cggactgctg gtggttttgg agtccttcgg gttcaaatac ggtgtgccga
     3301 acaacgccga ttttatgttc gatatgcgca gtctgcccaa cccgtattac gatcccgagt
     3361 tgaggcctta caccggtatg gacaagcccg tttgggatta tttggacgga cagccgttgg
     3421 cgcaggaaat ggttgacggc atcgaaaggt ttgttacgcg ttggttgccg cgtttggagg
     3481 atgaaagcag gagctacgtt accgtcgcca tcggctgtac cggcgggcag catcgttcgg
     3541 tctatattgt cgaaaaactc gcccgaaggt tgaaagggcg ttatgaattg ctgatacggc
     3601 acaggcaggc gcaaaacctg tcgggccgct aatcccgtca aaccattatg ccgtctgaaa
     3661 ccccgtccgc cgtttcagac ggcatttcaa atattgagaa gaaagaacag acatggcaat
     3721 ccaatggttt cccggccata tgaacaaggc aagaaaagct atcgccgagc gtgccaaaag
     3781 cgttgatatg gtgattgaaa tgctggacgc gcgtatgccc gcctccagcg aaaatccact
     3841 gttggcgcag ctttccaaag gtaagcccaa actcaaaatt ttaaacaagc aagaccttgc
     3901 cgaccctgaa cgcacaaagg tctggcttga ccactacaac agccgccccg acacccgggc
     3961 catcgccctc gattcctccg aaaccggcgc acacggcaaa atcacccaag cctgccgcgc
     4021 gatgattccc caccggcaag gcatagacaa acccctgcgc gttttgattt gcggcattcc
     4081 caacgtcggc aaatccaccc tcatcaatgg aatgataggc aaaaaatccg ccaaaaccgg
     4141 caacgaaccc ggcattacca aagccgaaca acgccttttc cttgccgacg acttctggct
     4201 gtacgacacc ccgggaatgc tgtggccgaa aatcatcgtc gaagaaagcg gctacaacct
     4261 cgccgccggc ggcgcagtcg gacgcaacgc attggacgaa gaagaagtcg ccctcgaact
     4321 tttagactac ctccgccgcc actacctccc tatgctgcaa gaacgctacc aagccgacaa
     4381 agaccccagc agccactggg acgacaactc gtggctcgaa tggatagcca aaaaacgcgg
     4441 cgcagtcctc agcggcggac gggtcaacta ccaaaaagcc gccgaaaaca tcctcacgga
     4501 cttccgcgaa ggcaaaatcg gcagaatcac ccttgaaaca ccgaaccaat gggaaacttg
     4561 gcttaaaaaa gcacgtcaga aagaagccga actcaaagcc attcgcgaag ccagaaaagc
     4621 agagcgcaaa gggcagaagc cttcggaagc ataaagaatg ccgtctgaaa aatatttttc
     4681 aggcagcttc tctctactcc aaccgatttc agacggcata tccaaaccca tgccgtttca
     4741 gcacggatac ccgtatgacc gacaaaattt ctcccgacgc gctgattgaa gccgcactgc
     4801 tgacccaaac cgaaccgctg accgaaaaat ctatgcgcga actgtgtgtg ccgccgttgt
     4861 cgcaagacaa actgattgat gtgttggcgc agttgaaaac gcgttggcag gatagggcgt
     4921 tgcaactggt gcatacgcag gagggctggc gttttcagat tgttcagacg gcattcgagc
     4981 ggctgggcag cctgcaagaa cagcgtgcgc cgcgctactc ccgcgccgtg atggaaacac
     5041 tggcgattat cgcctaccag cagcccgtaa cgcgcggcga catcgaaggt atacgcggcg
     5101 tggcggtgtc gcagaacgtg atgcagacct tgcaggatcg ggggtggatt gaagtcatcg
     5161 gacatcggga cacattggga aaacccgcat tgtgggcaac aacggcaact tttctcagcg
     5221 atttgcgctt ggacggtttg gaagaactgc cgccgctgac cgaactgggc gaactggttt
     5281 tgcccgattt gatagaaatg ccgcctacgg atgaagaaga gccggaaacc gtaccgtccg
     5341 ataccctgcc caactgaaat tccaaatgcc gtctgaaacg cacattgctt cagacggcat
     5401 tgcaacaaat aa
//
LOCUS       DAXFZR010000111         5247 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000111 DAXFZR010000000
VERSION     DAXFZR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.112"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            10..2154
                     /locus_tag="ACFJAS_001988"
     CDS             10..2154
                     /locus_tag="ACFJAS_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC3983150.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVPSKKPEARKDQGGYGFAMRFKRRNRHPMVIPKETEVKLNPNDWEATGLPTEPKK
                     LPLKQESVISKVQANNGDNNIYTSPYLTQSNHQNGNTGNGVNQPKNQAKGYENFQYVY
                     SGWFYKHAKPTIDQSQKKFQQGDDGYIFYHGKEPSRQLPASGKVTYKGVWHFVTDTKQ
                     GQKFNDILETSKGQGDKYSGFSGDEGETTSNRTDSNLNGNHEGYGFTSNLEVDFDNKK
                     LTGKLIRNNRVINATTGDKHTTQYYSLEAQVTGNRFNGKAKATDKSKNGETGDQTKQH
                     PFVSDSSSLSGGFFGPQGEELGFRFLSDDNKVAVVGSAKTKDKNANGNTAAAGTAGAA
                     GMSSEDTKLTTVLDAVELESDGKKVKNLDNFSDATRLVVDGIMIPLLPTESGNGQADK
                     GKNGGTDFTYTTTYTPESDKEDTQTGMAANGVQTVSNTAGGTSGKTKTHYKVQACCSN
                     LNYLKYGLLTRENNNSVMQAVENSNQTAARTAQGAQSMFLQGERTDEKKIPKDENVVY
                     LGTWYGHIATNGISWTREASNQENGNRAKFDVNFKGKKITGMLTAANRQEATFTIDAM
                     IEGNGFKGTAKTGDGGFAPDQNSSTGTHKVHITSAAVQGGFYGPNAEELGGWFAYPGN
                     GQTKNAQASSGNGNSAGSATVVFGAKRQELVK"
     gene            2241..5003
                     /gene="tbpA"
                     /locus_tag="ACFJAS_001989"
     CDS             2241..5003
                     /gene="tbpA"
                     /locus_tag="ACFJAS_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent transferrin-binding protein TbpA"
                     /protein_id="HFC3983151.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVEDGSNYAYFVVEGECPNGYAVCKDKPK
                     KDVAGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERTQ
                     QTFDTRDMTVPAFLTKAVFDENKKYGSIRGYGKYAGGHKYGGLITNGENGAQVGAEYG
                     TGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGSD
                     KYCRPSADKPSSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGYDRFGSDL
                     RHQDYYYQHANRAYSSKTPPQNNGKKTSPNGSNTSPNGSNTSPYWVSIGGGNVVTGQI
                     CLFGNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTG
                     THRTLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFN
                     KEAGIVFKGDFGNLEASWFNNAYRDLIVRGYEVQIKDGKEQVKGDPAYLNAQSARITG
                     INILGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVV
                     GSGYDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRDTKATARRTRPWYIVDVS
                     GYYTVKKHFTLRAGVYNLLNHRYVTWENVRQTAAGAVNQHKNVGVYNRYAAPGRNYTF
                     SLEMKF"
BASE COUNT         1483 a         1390 c         1345 g         1029 t
ORIGIN      
        1 gggtttgtta tgaacaatcc attggtgaat caggctgcta tggtgctgcc ggtgtttttg
       61 ttgagtgctt gtttgggcgg aggcggcggc agcttcgatc ttgattctgt cgataccgaa
      121 gccccgcgtc ccgcgccaaa gtatcaagat gttccttcca aaaaaccgga agcccgaaaa
      181 gaccaaggcg gatacggttt tgcaatgcgc ttcaagcggc ggaatcggca tcctatggtg
      241 attccgaaag aaaccgaggt taaactgaac ccaaatgatt gggaggcgac gggattaccg
      301 acagaaccca agaaactgcc attaaaacaa gaatccgtca tttcaaaagt acaagcaaac
      361 aatggcgaca acaatattta cacttccccc tatctcacgc aatcaaacca tcaaaacggc
      421 aacactggca acggtgtaaa ccaacctaaa aatcaggcaa aaggttacga aaatttccaa
      481 tatgtttatt ccggctggtt ttacaaacat gctaaaccaa ccatagatca atcccaaaaa
      541 aaatttcaac aaggcgacga cggttatatc ttttatcacg gcaaagaacc ttcccgacaa
      601 cttcccgctt ctggaaaagt tacctacaaa ggtgtgtggc attttgtaac cgatacgaaa
      661 cagggacaaa aatttaacga tattcttgaa acctcaaaag ggcaaggcga caaatatagc
      721 ggattttcgg gcgatgaagg cgaaacaact tccaatagaa ctgattccaa ccttaatggg
      781 aatcacgagg gttatggttt tacctcgaat ttagaagtgg atttcgacaa taagaaattg
      841 acgggcaaac tgatacgcaa taatagagtt ataaacgcta ctactggcga caaacatacc
      901 acgcaatatt acagccttga ggctcaagta acaggcaacc gcttcaacgg caaggcgaag
      961 gcgaccgata aatcaaaaaa tggtgaaacc ggggatcaaa ccaagcaaca tccctttgtt
     1021 tccgactcgt cttctctgag cggcggcttt ttcggcccgc agggtgagga attgggtttc
     1081 cgctttttga gcgacgacaa caaagttgcc gttgtcggca gcgcgaaaac caaagacaaa
     1141 aacgcaaatg gcaatactgc ggcggctggc accgcaggtg cggcaggcat gtcgtctgaa
     1201 gacactaagc tgaccacggt tttggatgcg gtcgagctgg aatcggacgg taagaaagtc
     1261 aaaaatctcg acaacttcag cgacgctacc cgactggttg tcgacggcat tatgattccg
     1321 ctcctgccca ccgaaagcgg gaacggtcag gcagataaag gtaaaaacgg tggaacagac
     1381 tttacctaca caacaaccta cacgccggaa agcgataaag aagacaccca aacaggtatg
     1441 gcggcgaatg gcgtgcaaac cgtttcaaat acggcgggcg gcacaagtgg caaaacaaaa
     1501 acccattata aagtccaagc ctgctgttcc aacctcaatt atctgaaata cgggctgctg
     1561 acgcgtgaaa acaacaattc cgtgatgcag gcagtcgaaa acagtaacca aactgccgcc
     1621 cgaacggcac agggcgcaca aagtatgttc cttcaaggcg agcgcaccga tgaaaaaaaa
     1681 attccaaaag acgaaaacgt cgtttatcta ggcacttggt acgggcatat tgccaccaac
     1741 ggcataagtt ggacccgcga agcctccaat caggaaaatg gtaatcgggc aaagtttgac
     1801 gtgaatttca aaggcaaaaa aattaccggc atgttaaccg ctgcaaacag gcaggaggca
     1861 acctttacca ttgatgccat gattgagggc aacggcttta aaggtacggc gaaaaccggt
     1921 gatggcggct ttgcgccgga tcaaaacagc agcaccggta cacataaagt gcacatcaca
     1981 agtgccgcgg tgcagggcgg tttttacggg cctaacgccg aagagttggg cggatggttt
     2041 gcctatccgg gcaatggaca aacgaaaaat gcgcaagctt catccggcaa tggaaattca
     2101 gcaggcagcg cgaccgtggt attcggtgcg aaacgccaag agcttgtgaa ataagcacgg
     2161 ctgccgaaca atcgagaata aggcttcaga cggcatcgtt cctgccgatt ccgtctgaaa
     2221 gcgaagatta gggaaacact atgcaacagc aacatttgtt ccgattcaat attttatgcc
     2281 tgtctttaat gactgcgctg cccgcttatg cagaaaatgt gcaagccgga caagcacagg
     2341 aaaaacagtt ggataccata caggtaaaag ccaaaaaaca gaaaacccgc cgcgataacg
     2401 aagtaaccgg tttgggcaaa ttggtcaaaa ccgccgacac cctcagcaag gaacaggtac
     2461 tcgacatccg cgacctgacg cgttacgacc ccggcatcgc cgtcgtcgaa caggggcgcg
     2521 gcgcaagctc gggctactcg atacgcggta tggacaaaaa ccgcgtctcc ttgacggtgg
     2581 acggcttggc gcaaatacag tcctacaccg cgcaggcggc attgggcggg acgaggacgg
     2641 cgggcagcag cggcgcaatc aatgaaatcg agtatgaaaa cgtcaaagct gtcgaaatca
     2701 gcaaaggctc aaactcggtc gaacaaggca gcggcgcatt ggcgggctcg gtcgcatttc
     2761 aaaccaaaac cgccgacgat gttatcgggg aaggcaggca gtggggcatt cagagtaaaa
     2821 ccgcctattc cggcaaaaac cgggggctta cccaatccat cgcgctggcg gggcgcatcg
     2881 gcggtgcgga ggctttgctg atccgcaccg gccggcacgc gggggaaatc cgcgcccacg
     2941 aagccgccgg acgcggcgtt caaagcttca acaggctggc gccggttgaa gacggcagca
     3001 actatgccta ttttgtggtc gaaggagaat gccctaatgg atatgcggtt tgtaaagaca
     3061 aaccgaaaaa agatgttgcc ggcaaagacg aacgtcaaac ggtttccacc cgagactaca
     3121 cgggccccaa ccgcttcctc gccgatccgc tttcatacga aagccggtcg tggctgttcc
     3181 gcccgggttt tcgttttgaa aacaaacggc actacatcgg cggcatactc gaacgcacgc
     3241 agcaaacttt cgacacgcgc gatatgacgg ttccggcatt cctgaccaag gcggtttttg
     3301 atgaaaataa aaaatacgga tcgataagag gctacggcaa atacgcgggc ggccacaaat
     3361 acggcgggct gattaccaac ggtgaaaacg gtgcgcaggt gggcgcggaa tacggtacgg
     3421 gcgtgtttta cgacgagacg cacaccaaaa gccgctacgg tttggaatat gtctatacca
     3481 atgccgataa agacacttgg gcggattatg cccgcctctc ttacgaccgg cagggcatcg
     3541 gtttggacaa tcattttcag cagacgcact gttccgccga cggttcggac aaatattgcc
     3601 gcccgagtgc cgacaagccg tcttcctact acaaatccga ccgcgtgatt tacggggaaa
     3661 gccacaggct cttgcaggcg gcattcaaaa aatccttcga taccgccaaa atccgccaca
     3721 acctgagcgt caacctcggt tacgaccgtt ttggttccga cctccgccat caggattatt
     3781 attatcaaca tgccaaccgc gcctattcgt cgaaaacgcc ccctcaaaac aacggcaaaa
     3841 aaaccagccc caacggcagc aacaccagcc ccaacggcag caacaccagc ccctattggg
     3901 tcagcatagg cgggggaaat gtggttacgg ggcaaatctg cctctttggc aacaatactt
     3961 atacggactg cacgccgcgc agcatcaacg gcaaaagcta ttacgcggcg gtccgggaca
     4021 acgtccgttt gggcaggtgg gcggatgtcg gcgcgggctt gcgctacgac taccgcagca
     4081 cgcattcgga cgacggcagc gtttccaccg gcacgcaccg caccctgtcc tggaacgccg
     4141 gcatcgtcct caaacctgcc gactggctgg atttgactta ccgcacctca accggcttcc
     4201 gcctgccctc gtttgcggaa atgtacggct ggcggtcggg cgataaaata aaagccgtca
     4261 aaatcgatcc ggaaaaatcg ttcaacaaag aagccggcat cgtgtttaaa ggcgatttcg
     4321 gcaacttgga ggcaagttgg ttcaacaatg cctaccgcga tttgattgtc cggggttatg
     4381 aagtgcaaat taaagacggc aaagaacaag tcaaaggcga cccggcttac ctcaatgccc
     4441 aaagcgcgcg gattaccggc atcaatattt tgggcaaaat cgattggaac ggcgtatggg
     4501 ataaattgcc cgaaggttgg tattccacat ttgcctataa tcgtgtccgt gtccgcgata
     4561 ttaaaaaacg cgcagaccgc accgatatcc aatcacacct gtttgatgcc atccaaccct
     4621 cgcgctatgt cgtcggctcg ggctatgacc aaccggaagg caaatggggt gtgaacggta
     4681 tgctgactta ttccaaagcc aaggaaatca cagagttgtt gggcagccgg gctttgctca
     4741 acggcaacag ccgcgataca aaagccaccg cgcgccgtac ccgcccttgg tatattgtgg
     4801 atgtgtccgg ttattacacg gttaaaaaac acttcaccct ccgtgcgggc gtgtacaacc
     4861 tcctcaacca ccgctatgtt acttgggaaa atgtgcggca aactgccgcc ggcgcagtca
     4921 accaacacaa aaatgtcggc gtttacaacc gatatgccgc ccccggccgc aactacacat
     4981 ttagcttgga aatgaagttc taaacgtccg aacgccgcaa atgccgtctg aaaggcttca
     5041 gacggcgttt ttttacacaa atttcaccgt tccccatccc tcccgataca ccgtaatcct
     5101 gaaacccgcc attcccgcgc aggcgggaat ccgggtctgt cgggtttcgg tttctccgat
     5161 agattctcgc tgcgttgggg tttctagatt cccactttcg tgggaatgac gattcagata
     5221 ttcctaatag caagccgccg gcgggca
//
LOCUS       DAXFZR010000112         5228 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000112 DAXFZR010000000
VERSION     DAXFZR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.113"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(436..1278)
                     /locus_tag="ACFJAS_001990"
     CDS             complement(436..1278)
                     /locus_tag="ACFJAS_001990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC3983152.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEECKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETRVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            1513..2433
                     /gene="pyrB"
                     /locus_tag="ACFJAS_001991"
     CDS             1513..2433
                     /gene="pyrB"
                     /locus_tag="ACFJAS_001991"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC3983153.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            2443..2901
                     /gene="pyrI"
                     /locus_tag="ACFJAS_001992"
     CDS             2443..2901
                     /gene="pyrI"
                     /locus_tag="ACFJAS_001992"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC3983154.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            2963..3629
                     /locus_tag="ACFJAS_001993"
                     /pseudo
     CDS             2963..3629
                     /locus_tag="ACFJAS_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4034..>5228)
                     /locus_tag="ACFJAS_001994"
     CDS             complement(4034..>5228)
                     /locus_tag="ACFJAS_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004466861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC3983155.1"
                     /translation="AAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLWGISGKYLYSP
                     WQWCRLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVVKISPDKEVSG
                     YGIPAVNANFYRVFMQHPQIVSRKETAAAPRLLSGPEGRLLYTKGARVYTKGLKEPGR
                     YLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEERLKDNEYYTR
                     THPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEPSRPVQAKIVS
                     VFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLTEEPKSRDTVE
                     LISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDNMPDQGRARVD
                     SDPFQ"
BASE COUNT         1138 a         1343 c         1573 g         1174 t
ORIGIN      
        1 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggaaccgat
       61 tcacttggtg cttcagcacc ttagggaatc gttctctttg agctaaggcg gggcaacgcc
      121 gtactggttt ttgttaatcc actataaata gcgagcatcg attcacggtg tactttagtg
      181 caaagcgtac aacgcgagcc tgaaccacca gccgcaatag gaaaagaaag ccgatagagt
      241 gcaatgcttg ctaacgcgca agcgagcttg caagaacgct tggctcaacg agagcaggca
      301 agacagaaag caggatagga gaggcaacgc aaaggtctcg ggctttgatt tcgccgtaaa
      361 cctgagtgcc gccttgtccg gacggggtgg ttcaggcggc gggtgccgac agggtgcaga
      421 tgaggaggag tattttcatt tggggtcgca acggaagtgg taggcgcaga tttcaaaacc
      481 gtttttgaaa tacaggcggt gcgcgtcggt acggtcgtgg ttgacgtgga cgttaaggtg
      541 gattttggta acccttgttt ccgcgctgat tttgcggact tcttccaaaa ggcgcgaggc
      601 gtagcctttg cggcggcttt gcggcagggt aacgatgtcg tcgatgtgga tgtggcggcc
      661 gccggctagg gtgcaggctt cgcggaagcc gcagacggcg acggcattgt gtttgcattc
      721 ttcaaaaata cccagcaggc ggtagccttg ggggcgttgg gttttgttga tctgttcggt
      781 aaagcggccg atgtcggtca gggcggaacg caaaacgctc aaggctgcaa aggcggtggc
      841 ggtgtcgtcc gcgccgattt cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg
      901 tgttttctcg gcggcgtgtt tttcttcgat tgcctgtgcc agcaggacgc gttcgttggc
      961 agggtcgttt tgtccgccct gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg
     1021 caggtcgttg tcggcggcaa agtccatcag gaagcggaac atctgcggat tgtcggtttc
     1081 cagtttttta tcgacggcga cgcagcggat gttgtccacc agcatggggc gcacccattt
     1141 cgaataggag aggcggttgt ctttggtgaa cgaggacagg atgccgcaca ggcgttccgc
     1201 ccagtcgccg gggcggaaaa tcttgccgga actcgttgtg ccgtggatga cgacttcgta
     1261 ggggttgcag attaacatgg cggcttcctg aaaagaaatg tctggcgcga ttatacctta
     1321 tgcttatgcg ggcgtgtttg gatatgccgt ctgaaaaata caggattcgt gcggtaaaac
     1381 tttgtggagg caaatgtgcg ataatacgcg ccgtgtcgcc gcttttgcga agctgttccg
     1441 caaacatacg ggcggcgtgg acgtataacc ggatacccgc ctgacgcggg ttttttacgg
     1501 aaggggggca aaatgcctaa tccgctttac agacagcaca tcatctccat ttcggatttg
     1561 tcgcgcgaac agttggaatg cctgcttcag acggcattga agctgaaggc gcatccgcgc
     1621 ggcgatttgt tggaaggcaa actcatcggt tcgtgctttt tcgagccgtc cacgcgcacg
     1681 aggctgtcgt ttgaaacggc ggtgcagcgt ttgggcggta aggtcatcgg cttttcggac
     1741 ggcgcgaata ccagtgccaa aaaaggcgaa acgcttgccg ataccgcccg tatcatttcc
     1801 ggctacaccg acgcgattgt ccaacgccat cccaaagacg gcgcggcgcg cgtggcggcg
     1861 gagttttcgc gcgtccccgt catcaacgcc ggcgacggca cgaaccagca tcccagccag
     1921 accttgttgg acctggtaac gatttatgaa acgcaggggc gtttggacaa gcttaaaatc
     1981 gccatggcgg gcgacttgaa atacgggcgc accgtgcatt cgctgtgtca ggcgttgaaa
     2041 cgctggggct gcgaatttgc cttcgtctcg ccgcccagcc tcgcgatgcc cgactacatt
     2101 accgaagagt tggaagaagc cgactgccga taccgcgccc tcggcagttt ggaagaggcg
     2161 gcggaatggg cggacatctt gtatatgacc cgcgtccagc gcgaacgttt cgacgaacag
     2221 gaatttgcca aaatccaagg caaattcaac ctcgaagcgt ctatgctcgc ccgcgccaaa
     2281 ccgaacctgc gcgtgctgca ccccctgccg cgcacggacg aaatccaccc cgatgtcgat
     2341 gccgcgccgc acgcctatta tttcgagcag gcgaccaacg gcgtttatgc gcgtatggcg
     2401 atattgtcgc tggtgttgaa cgaagaagtg tgaggaaccg atatggaagc gcaaaaactc
     2461 agtgtcgaag ccatcgaaaa aggcacggtt atcgaccata ttcccgccgg cagggggctg
     2521 accatcctgc gccagttcaa acttttgcac tacggcaacg cggtaaccgt gggcttcaac
     2581 ctgcccagca aaacccaggg cagcaaagac atcatcaaaa tcaaaggcgt gtgcttggac
     2641 gataaagccg ccgaccgcct cgccctgttc gcccccgaag cggtggtcaa caccatcgac
     2701 aatttcaagg tcgtacagaa gcggcatttg accctgcccg acgaaatcgc cgaagtgttc
     2761 cgctgcccga atcccaattg cgccggccac ggcgagccgg tcaaaagccg gttttatgtt
     2821 aagaaacaca acgggcagac gcggctgaaa tgccactact gcgaaaaaac ctacaaccgg
     2881 gattcggtgg cggaagcctg acggattccc gagcccggcg gcggaatttt gcctgccgcc
     2941 tgttttgcca atctgaaatg gaatgatgat gcacgcttct gtccaaagtc gtttcgcacc
     3001 gatactttat gttttgattt tctttgccgg ttttttgacc gcgcaaatct ggttcaatca
     3061 gaaagcctat actgaagagc tgcctccgct tctgtccgca ttgtccgccg tcgcgctggt
     3121 gtggctggcg tgggcgttcg tgtcggtgcg ttcaaaggct aaggcagaaa agttctaccg
     3181 cgaaaaaatg atacagaacg aaagcataca ccccgtcctg cacgcttctt tgcaacactt
     3241 ggaacacaag ccgcaaatgc tcgccctgct ggtcaaaaac cacggcaaag gcatggcgga
     3301 acaggtcagg ttcaaggcgg aagtgctgcc cgacgacgaa gacgcgcgca cgattgccgc
     3361 cgagttggca aaaatggata tgttcgcatt ggggacggac gcggtcgcct cgggcgaaac
     3421 ctatgggcgc gtgttcgccg atattttcga gttgtcggcg gctttggaag ggcgcgcgtt
     3481 caaagggata ctgaaactga cggcggaata taaaaaacat cttcggcgat gcctgccgtt
     3541 cggaaacggc gttggatttg ggcgcgctca atcaggcgtt gagggaaatc tcgaaaacgc
     3601 cggaaaagcc taagcagata ttttattgaa ggtgtcccga tgtatgggga gcgcacgggt
     3661 ggcgcggcgg caaagcggca agattgggtt tggatacccg gttggtttta tagtagatta
     3721 acaaaaatca ggacaaggcg gcgggccgca gacagtacaa atagtacgga atcggttcgc
     3781 ccggtgcttg ggcaccttag ggaatcgttc cctttgagcc ggggcggggc aacgccgtac
     3841 cggtttttgt taattcgcta tatctgtctg caagggagac agccgctgtg atttccgttc
     3901 gggcggaaac cgattccatc ggataaaagg cattttgtcc gactaaatca gggttttgca
     3961 tcaacgggat atgaaaacac agcctaaata aaaatgccgt ctgaatgctt cagacggcat
     4021 tgttgccgag gggttattgg aaagggtcgg aatcgacgcg ggcgcggccc tgatccggca
     4081 tattgtccaa atcccgtccc ggattggcgg cggtgtcgcc ttcggaaata tcggagatgt
     4141 tttccaaaat aatggcgtaa gccagtttcg gcgcagtgtg gtaaaccatt gccaagccca
     4201 cttcttcggc aggtgtcgaa atcagctcga cggtatccct gcttttaggt tcttcggtca
     4261 aattgttgga gaggttgacc tgcatcgttt tcttgcgttt gtaaaggctt aaaactgcgc
     4321 ctttgtccaa accgtcgtcc ccgcctttgt ctatcgtaat ggttttgaac tgtccgccga
     4381 cccccacgcc ttcaaacacg gaaacgattt ttgcctgaac cgggcgggac ggttcgtgcg
     4441 gcaccatatt gaagcggtcg gtgtcttccg gcattttcat caggtagtcg ccctgctgta
     4501 tttcggaaat ggcggtttcg accaccagcg gctgtatcga acgggtgcgc accggggtaa
     4561 tcaaaggatg ggtgcgggtg tagtattcgt tgtctttcag ccgttcttca gcctgtttcg
     4621 agcgttgttc gagggcggag tcggtatagt cgagggagcg cacgatgccg ctgaatgcga
     4681 cttcttgtcc gaggaattta cccgtatccg gatcggtgat gtttttattg attcggtagg
     4741 tcagataacg gcccggctct ttcaggcctt tggtgtaaac cctggcgcct ttggtgtaca
     4801 gcagcctgcc ttccgggccc gagagcaggc gcggcgcggc agcggtttct ttgcgggaaa
     4861 cgatttgcgg gtgctgcata aagacgcggt agaagttggc attgacggca ggaataccgt
     4921 atccggacac ttccttatcc ggactgattt tgacgacggg gatgccgtct gtctgttcca
     4981 aatcaaggcg cggttcgccg ccaacgtagc gcaacaccaa tacctggccc ggataaatca
     5041 ggtcgggatt gtggatttga tcccggttcg cgccccacag gcggcaccat tgccacgggc
     5101 tgtacaggta tttgcccgaa ataccccaca gggtgtcgcc ctgtttgacc gtgtagcgtt
     5161 gcggcgcatc cgggcgcacc tccagatttg ccgccaaagt ttgtgttgag aatgccatgc
     5221 ctgccgcg
//
LOCUS       DAXFZR010000113         5212 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000113 DAXFZR010000000
VERSION     DAXFZR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.114"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(61..258)
                     /locus_tag="ACFJAS_001995"
     CDS             complement(61..258)
                     /locus_tag="ACFJAS_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983156.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
     gene            complement(263..553)
                     /locus_tag="ACFJAS_001996"
     CDS             complement(263..553)
                     /locus_tag="ACFJAS_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983157.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            complement(598..1943)
                     /locus_tag="ACFJAS_001997"
                     /pseudo
     CDS             complement(598..1943)
                     /locus_tag="ACFJAS_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(2081..2503)
                     /locus_tag="ACFJAS_001998"
     CDS             complement(2081..2503)
                     /locus_tag="ACFJAS_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC3983158.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
     gene            complement(2509..3105)
                     /locus_tag="ACFJAS_001999"
     CDS             complement(2509..3105)
                     /locus_tag="ACFJAS_001999"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC3983159.1"
                     /translation="MKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAEV
                     ISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTGL
                     HSIDGRELIEKAFGSKKSMLLLTNNKTQAEQNLEDGLEQLACGTWTGFRNPVQHELRA
                     NLSPSIFNDKDALDLISLVSYLLRKVEQTKKRAKPTSP"
     gene            complement(3295..4152)
                     /locus_tag="ACFJAS_002000"
     CDS             complement(3295..4152)
                     /locus_tag="ACFJAS_002000"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3983160.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFRLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVKPNTENGTTIT
                     LTELTKKQGYPLDNYVGHLSRLFDFPAQDFKIKVSLNGSEPRIIDGNLKYNLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHF"
     gene            complement(4166..4537)
                     /locus_tag="ACFJAS_002001"
     CDS             complement(4166..4537)
                     /locus_tag="ACFJAS_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3983161.1"
                     /translation="MKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWW
                     DKPAPTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIG
                     NAVPPMYTEKIGRAIVDNIEC"
     gene            complement(4534..5142)
                     /locus_tag="ACFJAS_002002"
     CDS             complement(4534..5142)
                     /locus_tag="ACFJAS_002002"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC3983162.1"
                     /translation="MQVLAGIDYELSCKETYEANINAKFIQADIFELQPETLEKELGL
                     KKNDDDLILIGCSPCQYWSVIQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGI
                     LSRMKESGLDNFIKLLEEKGFTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSS
                     IRANGLRYAMFWEWKTAFPKLWQDIKTRRILCIVVRDYPISI"
BASE COUNT         1347 a         1184 c          980 g         1701 t
ORIGIN      
        1 aaaaagtgtt tggcggtaat ggatggagag tttttcagac gaccgccgaa catccgaaaa
       61 tcagtctttc aaaaatccga atacgacaaa ttcgcattgg ttgccgattt cttccaaacc
      121 tgcgttaacc gcttcttcga agtcgtagaa ataatcggca ttggtaatta atttggtatg
      181 tccgatgtcg cccgtttcag gagagagata cagaaagtcc cctgttgata cggactggac
      241 aacatagact ttctgcattc aatcagcctt tcttaacgaa ttgaaaaccg gtgactttca
      301 gtttttgagt tttgcccgta gtgacgattt ccacgttcag gtttgcttcg atcggaaatt
      361 gggcgtttcg gaactgctcg aaattggcag agccgccgaa atcgtattca gtagtagagc
      421 tgcccaatgc gttgccttgg gagctgtcta agggtgtggc gacaatcagg cggcaatagt
      481 cgaagctctt gccttcgatt tgtccgttga attttttaac gccgacgatg tggccttgaa
      541 gttggatgtt cattttttgg tttccttgtg tgattaaacg tctttcgggc agacacttta
      601 agcccatgaa atcggtagtc ttgcgaattt gtcgtaaatg aagttgttat agctttcttc
      661 attgttgacg tgtttttgct gttcaagctg tttttcaaga ttctcgtaat attcgtacat
      721 ataataaggg tcattgtacg gtttgaatgc gggctgttca tgaatgactt gagatttcaa
      781 aaaggcgcag tcgtaggctt cgagagccaa agacttgggc agcttgtgat gactcggctc
      841 aatcagttca aacagtttgg ctttgtccaa ttcgggaaaa atgaatttca gaccgtttgc
      901 cgcacgtccg aactgttttt ttacccattc aaggcagcgg tcggctgaaa caaccttatc
      961 ttccttaacc gcgtgtatgc gcgttgcctt ttgggcgaat cgttcgcaaa tgggatatgc
     1021 gccgccgaaa tattcgcccg gattctgcaa aacttcgaaa gggataacga tgtcttttgc
     1081 tttgaattca atttcaaagc gcgtccatgt gcttgtttta tcgcccaact gcttgccttt
     1141 ttcatagacg cggacatatt tggacgattc acgggagccg ataccatagg tcttgccttt
     1201 ggtcattttg gcttcatcgt cttcttccca atccgacccc aaacattcgc ccttcggttt
     1261 gacgtgatgg cacgtaaaca aacctttatt gcggtcttct cttgcctgat tcgggctgta
     1321 ctctccgttg aaaaagtctt ttgcaatgtc aacgcgcgtt atttttggac gaatcgcatt
     1381 tgtcagaaaa tcaaaaagcc ttgattccca tccaatattt gcagcattgc agcctaccgc
     1441 cgtcaattca accaacatcg tttcacgttg gccgccgtaa tggacgcgcc cgtattgggc
     1501 attttccgtt cccatcagcc agcagccttc atagaaacgc ccgcccgaat gcttggcttt
     1561 ttcaatgatt ccgaatccga aaatttcctc catcttggcg gaagccgcgc gtatgaaatc
     1621 gtcatcatcg aacaaatcaa acgaaagtcc ataaacatgg aaaaacgtgt cttcatgaat
     1681 tgaaaatgtg atttggtcaa tgaaagccga atctgataca ccgcgcctaa gaggaacgcc
     1741 taacaggttt cctttaccgt ccgttatgta cgtttcgtaa cattcgaaga cttcctgaac
     1801 cctgcccgcc gtttcggttt ccgtaccccc ctgttagata aggggggggg aagattcgaa
     1861 gcggttgccg gcttcctgcc gtccgccggc gcctccgtca tcacgccggc aacctccttt
     1921 gtcatccctt gcttatcttc catttgcgaa tcctcaaaaa cgggcaaaaa aaagccctgt
     1981 tacttgtaga aagtaaaggg cgttaatttt tgttaatcac cccttcttag ggacgcaata
     2041 tataagggtc ttaattaaag gaagatgccg tctgattttt tcagacggca tcttttattt
     2101 cctcgaccaa ccgatttaat cgctccctac aaatagcttt attttttaat tcgcactccc
     2161 aaacgacaat cactttgaaa ccaatctttt ccagctcggt ttcattttta atatcacgtt
     2221 cgcgattttt tgtgattttt tccaaccaaa aatccatatt gcttttcggg atatgtcctt
     2281 tattacaaga atgcccgtgc caaaaacagc cgtgtataaa cacgactgtt ttatatttag
     2341 gcaaaacgat atccggtttg cctgcatagc gcttatcgtt tttccgataa cggaaacctt
     2401 gggaaaaaag aaatttcctt accaatactt ccggtttggt ttctttgcta tggatttttg
     2461 acatcacgaa ggaacgtttg gatggagtga aaatatcggt cataaaattt aaggcgatgt
     2521 aggtttagct cgtttttttg tttgttccac ttttctcaat aaatatgaaa ctaagctgat
     2581 taaatccaaa gcatccttgt cattaaaaat actaggagat aaatttgctc ttaactcatg
     2641 ttgcacagga tttctaaatc cagtccatgt tccgcatgca agctgttcaa gaccatcttc
     2701 taaattttgt tccgcctgcg ttttgttatt cgttaataga agcatagatt ttttactccc
     2761 gaaggctttt tcaatcaatt ctcttccgtc tatgctatga agacctgtct gcttctgaac
     2821 cttatcttcg aaaactttaa ctgcctcgat tgcagcactt aaataatcct tcttttcatt
     2881 gtaaaaatcg ttacaggcag tatgcaaatc ctgatgcaaa tgccgccaat gatagtaagg
     2941 atattctgga ataatactat gtaaggcaga gattacttcc gcttgttctt tatttgtaag
     3001 ctccgaatct tccaaacgat tcaaaataac ttctaccggt tctctttcct tttctggcaa
     3061 tttgtttttc caattttcga tatcaactcc gctttcacct ttcacttcgt aatctttctc
     3121 tttttttctt ctttttctcc aatcttgccc gatggctcag acgatttccc caaggaaaac
     3181 ccgtaatttt gaagttttag gatgcttcca atccaatgat gtcctatctg tagcgattaa
     3241 atcttcacca tcattatcta taaaatcaat acttaaccat cctgtcagat aggattagaa
     3301 atggctggat tcgctatcag tgaaaaactc gggcatattt accattctgc cgttggcaaa
     3361 caaggtaata cctttcatat tattctttaa aggtttttcc gttgtaatga acttaccttg
     3421 tattaatccg ctgtattcat actgttcgaa ttttgaagat aacgatgaaa tattggttgc
     3481 tagatcctgg tattcccatt cgaattgtgg ggtaacaaga ttatatttta ggtttccgtc
     3541 aatgattctt ggttccgagc cgttcaagct tactttgatt ttaaaatcct gagccggaaa
     3601 atcaaataaa cgggaaagat gccccacata attatctaac gggtatcctt gttttttcgt
     3661 cagctcggtt aaagttatag ttgttccgtt ttcggtattg ggtttaacag actctttctg
     3721 aaactccggt tgataaatac gctcactttt tttaatctct gcataatcca aagtaaaagt
     3781 aacccgttcg tttccttgga tagtagagat ttcgattttg ttgccaagcc tgaataatgc
     3841 caatttacca agaccttttt tacccgttgg aattcttccg cacggggagg cttgtttttc
     3901 ttcccttctg ttccgaccga ttctcaaata aaaatcattg atttcatcga agctcattcc
     3961 tatgccatta tctttaataa cgattttatg ctccggttta tcgaataacc taacttccac
     4021 ttccgtagca catgcatcgt aggcattcgc tatcaactcg gcaatcgcag gaacagtgtg
     4081 cgaatacatc tttacaccca aatgttcgat tacggttggc tcgaaactca ttttcaattt
     4141 ttcttcagac atgttttctc cttttctaac attcgatatt atcaacaatt gccctgccta
     4201 ttttttccgt atacatcgga ggaacggcat taccaatcaa acgggctatc ttgtccctac
     4261 tgcccgcttt aaagacatag ttacgaggga acgattgcaa tgttgcacct tctcgcaggg
     4321 ataacgccct atcttcttca ggatgggcaa accgcccgtt ggaaatgcta aagaatttgg
     4381 tcgttatggt aggtgcgggc ttatcccacc atagcctacc gaacgtgtct ttaaaactat
     4441 tgtcttttcc gataaaacat tccagctgta attcgggaat atgcgcaaaa gccaaacggt
     4501 ttcctccgtt tttaggtatc aaagccaatc gtttcaaatt gatatcggat aatcccgcac
     4561 aactatgcat aaaatccgtc tcgtcttgat gtcctgccat aattttggga aagccgtttt
     4621 ccattcccaa aacatcgcgt accgtaagcc gtttgcccga atacttgact ggttccagct
     4681 ttttttggtt attctgtttg caattaacgt aaatctttta cggctttggg gaattccgta
     4741 atcagccgta ttgtgaatac cgaaatggac ggtaaatcct ttttcttcca ataactttat
     4801 gaaattatca agcccgcttt ctttcattct gctcagaatt cccggtacgt tctctacaac
     4861 aacatatccg ggattgaaat attctacaaa ccgctgaaac tccaaaagca aactcttgga
     4921 tttttccgat tttcttttgt ctgtttgaat aacactccaa tattgacaag gactacaacc
     4981 tataagaatc aaatcatcat cattcttttt taagccgagt tctttttcca aggtttcagg
     5041 ctgcaattca aaaatatcag cctgtataaa tttggcattt atattggctt catatgtttc
     5101 tttgcagcta agttcgtaat ctatcccggc taaaacctga ataccggcgg attacattcc
     5161 atagctcatt ccgcctccgc cacagaaaaa atcaaccgcc tttaacttac tg
//
LOCUS       DAXFZR010000114         5209 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000114 DAXFZR010000000
VERSION     DAXFZR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.115"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            105..926
                     /locus_tag="ACFJAS_002003"
     CDS             105..926
                     /locus_tag="ACFJAS_002003"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC3983163.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1012..1674)
                     /locus_tag="ACFJAS_002004"
     CDS             complement(1012..1674)
                     /locus_tag="ACFJAS_002004"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC3983164.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1712..2539)
                     /locus_tag="ACFJAS_002005"
     CDS             complement(1712..2539)
                     /locus_tag="ACFJAS_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC3983165.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGHAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRL
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2922..3545
                     /gene="lolA"
                     /locus_tag="ACFJAS_002006"
     CDS             2922..3545
                     /gene="lolA"
                     /locus_tag="ACFJAS_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC3983166.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3818..4957
                     /locus_tag="ACFJAS_002007"
     CDS             3818..4957
                     /locus_tag="ACFJAS_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC3983167.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKMRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPTKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            5052..>5209
                     /locus_tag="ACFJAS_002008"
     CDS             5052..>5209
                     /locus_tag="ACFJAS_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3983168.1"
                     /translation="MNLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKY"
BASE COUNT         1211 a         1408 c         1444 g         1146 t
ORIGIN      
        1 tgttttttgg ggtgtaatcc gaggtagggg cggctggggt gcttctccct tgtctgccgc
       61 tgctgttatg atgatatttt tattctgtat ttaaggaggg ggtgatgagc agtccgcgcc
      121 aagtgtttta tatttccgac cgcaccggtc tgactgccga gaatatcggc gaggctttgc
      181 tgaaccagtt tggcaatctg tcgttcaaac gccatacgca tccgtttgtc gatacgccgg
      241 aaaaggcgcg cgcggtggtg gagaaggtca atcggagccg gcaggaaaac ggtcagcgtc
      301 cgattgcgtt tgtcagtgtg gttgatgacg aaatccgccg gattattaaa ggggcggatg
      361 cttttcagat taatttcttt gagacttttt tgggactgtt ggagaaggaa ctcaataccg
      421 aagcaacggt atccgggcag gggcatcaca gtatcggcaa tacgaagcgt tacgatgcgc
      481 gtatggaggc ggtcaatttt tctttgaacc atgatgacgg ggtcagcgat aagaaccttc
      541 aggaggcgga tgtgattctg atgggcgtgt cccgttcggg caaaacgccg acctgcctgt
      601 atctggcgtt gcaatacggt atccgtgcgg caaactatcc gctgattccc gacgatttgg
      661 aatcggccga tctgccgcgt atggtcaagc cgtataaaga caaactgttc gggttgacta
      721 tccagccgga acgtttgcag gccatccgtc aggaacgccg cccgaattcg gcttatgcgc
      781 gcatcgacac ctgccgcagc gaagtggcgg acgcgcagag tatgttcaga cggcatggca
      841 ttccgtttgc gaatacgacg gacaagtcgg ttgaggaact ggcggtacac atccttcagg
      901 cgtgcaagct caaacgcagg ttttgacggg ctttgattcg gtttgaaggt ggaaatgccg
      961 tctgaaatca ggtttcagac ggcagtttta tgtttgtggg gcggatattt ttcagggctg
     1021 tattttgtcc agacattcga gcagatcgag cggcgtgcgg atgtggaaat ccgcctgcca
     1081 tgagccggta tcgtcttcat cggaaatata gccccattcg gcgaggacgg ttttcatacc
     1141 ggcgttgcgt ccggcttgga tgtcgcgttc cgcgtcgccg acgtagaggg tgtgttgcgg
     1201 gtcggcgtgg atttttccgc aggcgtgcag catgggtttg atgctgggtt tgggttcgcc
     1261 gcaggtgtcg ccgctgacga cggtggcggg cggaacggcg aatccgagtt tgggaacgag
     1321 tttgtcggtg aagcgcatgg gtttgttggt gatgatgccc catttgatgc cgcgtctgtc
     1381 gagttcggcg atgagttcgt tcacgccgtc gaagagagtg gtgtcttggg cgtagcggct
     1441 gtcgtattcc tccagatatt cggtgcgcca tgcggtatag tcggggtgtt cgggcgtgat
     1501 gttcgcgccg agtttgagca atcctgccgc gccgtggctg gcttgggtgc ggatttcgtc
     1561 catgcttttt tcgggcagtc cgtggcgggc gagttgggtg ttgagtgcgc cgccgaggtc
     1621 tagggcggtg tcggcgagcg tgccgtcgag gtcgaacaat acggcttgta tcatgtgttt
     1681 tcctttttta taaagtgcgg gacgaaaggt ttcagacggc atgtttattt tgtttcaaac
     1741 cctgctcgaa atcttccaac atatccaatt cgaagcggct gaagcccgct ttttcccgcg
     1801 cttcgatgtt cacatagccg cggaagataa acatatcgta acgggcaatc agactgcgga
     1861 acagggcgac aggttccaaa ccgcgttcgc ggcagaggtg ttgataccac cggttgccga
     1921 tggcgacgtg tcccacttcg tcgcggtaaa tgatgtccaa cacgccgcag gttgccgaat
     1981 cgccgcgctg ctccaccttc gcgcgtatcc ccggcgtaac gtccagtccg cgcgcttcca
     2041 acacgcgcgg caccaacgcc agacgcaaca aaggatcgta ggcggttttg tatgccatat
     2101 cccataaatg attgtgtgcc tcgaaatcgc cgtaatcgaa gccgaaagca cgcagccttt
     2161 cgcgcatcag gcggaagtgg tacacctcct ccttcgccac tttcacccag tcgcggacaa
     2221 actgaaacgg cagcgtgcgg aaacggtatg ccgcgtccaa agccagattg acggcgttga
     2281 actcgatatg cgtaatcgcg tgcagcatag ccgcatggcc ttcggttgtg ttcattttgc
     2341 gcggggtcag ttgcgagggc gcgaccaaaa ccggcttgtc cggccgtccc gcgcggggga
     2401 agtccgccgg cggcgcgttt gcctccgccc cgtccgcatt ttgaacggcg gcaaacgcct
     2461 catccgtcag ccgtcctttt tcatcggggt cgcccgaaag cagggcgcgt tccagcaaag
     2521 cataaatatc gggtttcatc tcaagtccgc cgtgttcgga aaacgaatat tatagcgttt
     2581 aaaaaaaaca agatgaggca tataatctcc gcgattcggc attccgcgcc caaaccgtca
     2641 aatatagtgg attaacaaaa accagtacgg cgttgccccg ccttgccgta ctatgttgta
     2701 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataac gcggcataca
     2761 attgaaaggg cagcgtggcg tgcctgcttt ttccgagcgg tcaaaaaaat cagccctcgg
     2821 aaaacgcggt ttgcaaaatg caaaccgccc gtaacgccgc ccgtatgatt gttttgccgc
     2881 gccgatactt tgcgccacac ccatcccgac aaggaaaaat aatgatgaaa ccgcacaacc
     2941 tgttccaatt cctcgccgtt tgctccctga ccgtcgccgt cgcttccgca caggcgggcg
     3001 cggtggacgc gctcaagcaa ttcaacaacg atgccgacgg tatcagcggc agcttcaccc
     3061 aaaccgtcca aagcaaaaag aaaacccaaa ccgcgcacgg cacgttcaaa atcctgcgcc
     3121 cgggcctctt caaatgggaa tacactttgc cctacaaaca gactattgtc ggcgacggtc
     3181 aaaccgtttg gctctacgat gttgatttgg cacaagtgac caagtcgtcc caagaccagg
     3241 ccatcggcgg cagccccgcc gccatcctgt cgaacaaaac cgccctcgaa agcagttaca
     3301 cgctgaaaga ggacggttcg tccaacggca tcgattatgt gcgggcaact cccaaacgca
     3361 acaacgccgg ctaccaatac atccgcatcg gcttcaaagg cggcaacctc gccgccatgc
     3421 agcttaaaga cagcttcggc aaccaaacct ccatcagttt cggcggtttg aataccaatc
     3481 cccaactctc gcgcggcgcg ttcaagttta ccccgcccaa aggcgtggac gtgttgagca
     3541 actgatgccg gccgccccga tgccgtctga acgccgccga ggcttcagac ggcattttca
     3601 cgcaggcgga acaatgtccc gcattaccgc ccgatcgggc aacggaacgg caaatccgtg
     3661 aaaattaacg gttgcgcccc ggctgttttt gccgtttaat gcaaaccttg ctgcaccaag
     3721 ggccaagaaa gccgaccggc cgcccccaca gcttccgatg caggcggccc gtccgtccct
     3781 gcaatgtttt ttatttttga acgaaaggtc gaaaaccatg aaaaaaacac tggtggcggc
     3841 ggcaatcctg agccttgcct tgactgcgtg cggcggcgga agcgataccg ccgcccaaac
     3901 cccctccgcc aagcccgaag ccgaacaatc gggcaaactc aacatctaca actggtcgga
     3961 ttatgtcgat cccgaaaccg tcgccgcctt tgaaaaagaa accggcatta agatgcgttc
     4021 cgactattac gacagcaacg aaacactgga ggcaaaagtc ctgaccggca aatccggcta
     4081 cgacctgacc gcgccgtcca tcgccaacgt cggccggcaa atcaaagcgg gcgcgtatca
     4141 gaaaatcgac aaggcgcaaa tcccccatta cggcaacatc gataaagatt tgctgaaaat
     4201 gatggaagcc gtcgatccgg gcaacgaata cgccgtcccc tatttctggg gcatcaacac
     4261 cttggcaatc aacacgcggc aggtgcaaaa ggcattgggt acggacaagc tgcccgaaaa
     4321 cgagtgggat ttggtgttca aacccgaata caccgccaaa ctcaaatcct gcggcatcag
     4381 ctatttcgac agcgcaatcg aacagattcc cttggcgttg cactatttgg gcaaagaccc
     4441 caacagtgaa aatcccgaag acatcaaagc cgccgtcgat atgatgaaag ccgtccgagg
     4501 cgacgtgaaa cgcttcagct cttccggcta tatcgacgat atggcggcgg gcaacctgtg
     4561 tgccgccatc ggttacggcg gcgatttgaa cattgccaaa acccgtgccg aagaagccgc
     4621 aaacggcgtg gaaatcaaag tattgacccc gaaaaccggc gtaggcgtgt gggtggattc
     4681 ctttatgatt ccgcgcgacg cgcaaaacgt tgccaacgcc caccgctata tcgactacac
     4741 gctccggccc gaggtggctg cgaaaaacgg cagcttcgtt acctacgcgc ccgccagccg
     4801 tcccgcgcgc gagctgatgg atgaaaaata cacctccgac gcatcgattt tcccgaccaa
     4861 agaactgatg gaaaaaagtt tcatcgtatc acccaaatcc gcagaatccg tcaaactggg
     4921 cgtgaagctg tggcaagggc tcaaagcggg caaataaccg gaatccctgc cgtctgaaac
     4981 ctttcgggcg gcaggaaacg gcgtgtccgc attaagtcag gattaggatt atttagaaag
     5041 atgagatgga tatgaattta agtattgtcg ttcctattta taatgtcgaa agttatttgg
     5101 aagcgtgttt aaattctata gaacctatat taagtaatga aaatgtcgaa cttattcttg
     5161 tgaatgacgg gtcaaaagac ggaagtgaag atatatgtta taaatatat
//
LOCUS       DAXFZR010000115         5173 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000115 DAXFZR010000000
VERSION     DAXFZR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.116"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            93..1103
                     /gene="trpS"
                     /locus_tag="ACFJAS_002009"
     CDS             93..1103
                     /gene="trpS"
                     /locus_tag="ACFJAS_002009"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC3983169.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLSAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            1184..1729
                     /locus_tag="ACFJAS_002010"
     CDS             1184..1729
                     /locus_tag="ACFJAS_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983170.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            2046..2390
                     /locus_tag="ACFJAS_002011"
     CDS             2046..2390
                     /locus_tag="ACFJAS_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983171.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAAEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAA
                     QEAADKMKDAAK"
     gene            complement(2600..4108)
                     /gene="ppx"
                     /locus_tag="ACFJAS_002012"
     CDS             complement(2600..4108)
                     /gene="ppx"
                     /locus_tag="ACFJAS_002012"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC3983172.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRAFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGGNEMLWYAVLSLRLAALFCRSRQDLGFP
                     KNMQLRTDTESCGFILRIDSKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(4271..5108)
                     /locus_tag="ACFJAS_002013"
                     /pseudo
     CDS             complement(4271..5108)
                     /locus_tag="ACFJAS_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
BASE COUNT         1231 a         1358 c         1430 g         1154 t
ORIGIN      
        1 agtctgttgt cggctggatg tatgtggcaa atcgggtata atcggcgcat cttttccctt
       61 tcagacggca ttgctgccgt aaggacattt ttatgagcaa aaaacgagtt ctgaccggcg
      121 taaccaccac cggcaccccg catctgggca actacgtcgg cgccatccgc cccgccgtcc
      181 gcgcggcgca aaaccccgat accgaatcct tccttttcct cgccgattac cacggcatca
      241 tcaaatgcca cgagcaggag atgattcacc aatccaccca agccgttgcc gccacttggc
      301 ttgcctgcgg actcgacccc gagcgcacca ccttctaccg ccaaagcgac attcccgaag
      361 tgatggagtt gaactggatt ctgacctgca tcaccgccaa gggtttgatg aaccgcgccc
      421 acgcctacaa agccgccgtt caagccaatg cggaaaacgg gcaggaagac cccgatttcg
      481 gtgtggaaat gggtttgttc agttatccga ttctgatgac tgccgacatt ttgatgttca
      541 acgccaacga agtccccgtc gggcgcgacc aaatccaaca cgtcgaaatg gcgcgcgaca
      601 ttgccggccg cttcaaccac cgtttccaag aactcttcac cctgcccgaa gtgaaaatcg
      661 acgaaaacgt cgaactcttg gtcggtttgg acggacgcaa aatgtccaaa tcctacggca
      721 ataccattcc gctttgggaa aacgacaaaa aaacccaaaa atcggtcaac aaaatcatca
      781 ccaacatgaa agagccgggc gagccgaaac agcccgacga aagccctttg tttgaaatct
      841 ataaagcctt ctccacgccg tctgaaacgg cggaatttac gcaaatgctt gccgacggct
      901 tggcttgggg cgaggcgaaa aaactttcgg cggcgaaaat caacgccgaa ttggcagaac
      961 tgcgcgaacg ctacaacgca ttgacctcta atccttcgca aatcgaagag attttgcagg
     1021 caggcgcgca aaaagcgcgc aaagaagcgc gcgaattgtt ggataaagta cgcgatgcgg
     1081 tcggcatccg cccgttgaaa taaacccaat gccgtccgaa cctccgcctg ccacgtttca
     1141 gacggcattt cagacggcat tttgaaacca tcaggagtgc ggaatgctga aaataccttt
     1201 tgccgtgttg ggcggctgcc tgctgcttgc cgcctgcggc aaatccgaaa atacggcgga
     1261 acagccgcaa aatgcggcac aaagcgcgcc gaaaccggtt ttcaaagtca aatacatcga
     1321 caatacggcg attgccggtt tggctttggg acaaagtagc gaaggcaaaa ccaacgacgg
     1381 caaaaaacaa atcagttatc cgattaaagg cttgccggaa caaaacgccg tccggctgac
     1441 cggaaagcat cccaacgact tggaagccgt cgtcggcaaa tgtatggaaa ccgacggaaa
     1501 ggacgcgcct tcgggctggg cggaaaacgg cgtgtgccat accttgtttg ccaaactggt
     1561 gggcaatatc gccgaagacg gcggcaaact gacggattac ctgatttcgc attccgccct
     1621 gcaaccctat caggcaggca aaagcggcta tgccgccgtg cagaacggac gctatgtgct
     1681 ggaaatcgac agcgaggggg cgttttattt ccgccgccgc cattattgag ttattcggac
     1741 atcacggaat atatttggat ttttcaaacc ttgcggaaaa agtccgcgcc gccgggaaac
     1801 tcaaaaggag gggatgtgtg ttacaatttt ccgaactgtt ttcataaaat agttttcgga
     1861 cgtgtttcaa tatggcattg atgccgcctt gttgttcgag aaaaaaacct ttatatttaa
     1921 tataatgggt tttaactaaa cgggaaaccg ttttctctcc ggccgatggg caaaatcagc
     1981 cgattgatgg aacacggtcc ggtttttaag caaaacctta atcaacaact caagaaggat
     2041 aagacatgaa aaaattattg attgccgcaa tgatggcggc tgccttggca gcttgttcgc
     2101 aagaagccaa acaggaggtt aaagaagcgg cccaagccgt tgagtccgat gttaaagaca
     2161 ctgcggcttc tgccgccgag tctgccgctt ctgccgccga agaagcgaaa ggccaagtca
     2221 aagatgctgc ggctgatgca aaggcaagtg ccgaggaagc tgtaactgaa gccaaagacg
     2281 cggcagccga aaccaaagaa gcggtaagcg aagcggctaa agacactttg aacaaagctg
     2341 ccgacgcggc tcaggaagcg gcagacaaaa tgaaagacgc cgccaaataa tttgttgcct
     2401 tggcaaaaat gacgagatgc cgcctgccgg caacccaaat gaaaccgcct gaagattttc
     2461 aggcggtttt tgagttgtat gccccttgtt ttttacgcct gcatgaccgt gcagggagga
     2521 tgtgccgcgc atatcgggat attgcggaca gggaaatgcc gtctgaacat catttagacg
     2581 gcatttggtt tgtcaaggtt cagacggcct cgactttgaa cggcatattg attttttgcc
     2641 attggacgct ttcatattcc aatgcgtcgg caatcagggg gtggcgttcc agccatttac
     2701 tgtcaatacg caggatgaag ccgcagcttt ccgtatccgt acgcaactgc atatttttcg
     2761 ggaaacccag atcttggcgc gaacggcaga acagggcggc aaggcgcagg gacaaaacgg
     2821 cataccacaa catttcgttg ccgccgataa tgccgcccat ttttttcata tcgccgcgat
     2881 gaccgatgac cagttgggca agtatggtct gttccttgcg tgaaaaaccc ggcatatcgg
     2941 cgttttcgag gatgtaggcg gaatgcttgt gatagccggt gtgggcgatg tccaaaccga
     3001 tttcatgcag cgcggcggcg cgtccgagat actgttgcca caaggcaagt tcttgaaccg
     3061 taacgttttt ggcatgacag aggctgtcca taaaggtttg cgcggtctcg gcggtgcgtt
     3121 tcgcctgatt gaggctgacg tggtagcggt gttggaactc ggcaaccgtt tgtccgcgca
     3181 tatcttcgtt taagccgcgc ccgatcaaat cgtaaaacac gccgtcgcgc agggcggctt
     3241 cggttacggt catcctgtcg agtttcattt cctcaaacgc cgccatcatc acggcaagtc
     3301 cgccggcaaa aacttcgatg cgttccggtt tcaggttttc aaatttggct tttttgaccg
     3361 aaccggcttc gatgatgcgt tcggcgaggg cgcgcattcc cttgtaggta atgtccgcct
     3421 cttggggcat ttcggcggca agcacgtcgc ggatggattt tgccgaaccc gatgtgccga
     3481 cggcgaaatc ccaaccttcg cgcctcatat ttttgctgat gcgctggatt tcgttgcggg
     3541 cggcggaaat ggcggcttgg aagtctttgg cggtgatttt gttttggaag aagcgcaggc
     3601 tgtaggttac gcagcccaag ggcaggcttt cggtaatgtc gggattcagc gtcgaaccga
     3661 tgacaaattc tgtcgaaccg ccgccgatgt cgataaccag cattttgccc ccgcaggggg
     3721 ggagggtgtg gatcacgccg gtataaatca gccgcgcctc ttcgcgcccg gcgatgattt
     3781 cgatggggaa acccagtgct gcttcggcct tgggaaggaa atctgcgatg tttttggcga
     3841 cgcggaatgt attggttgcc acggcgcgca cctgctcggg acggaaggcg cgcaggcgtt
     3901 cgccgaattt tgccaggcag tccaaagcct gttcctgcga ggatgtgctg aggtttttct
     3961 gttcgtccag accggcggca aaacggacca tctgtttgaa ggaatcgatg acttttaatt
     4021 gtccgttgtt gttttcgcaa atctggaggc ggaaactgtt ggaacccaaa tcgacggagg
     4081 cgaggacgtt tgcgggattg gtggtcatgg cggataccgg tgggggaaaa acgcaatgtt
     4141 actctgacgg cgcaggcgtt gacaataaat gatgcggctg tttttgattc tgctcacgga
     4201 tgttgccgac ggtatgtttc cgacagcaaa aaatgccgtc tgaaaaacgt tcggacggca
     4261 ttttttgcgc ttatttgaaa tccttttcca cgctcatgaa aatctgcgta tttttgcgtg
     4321 tgtaaaaact tttcatattg ctgccgattt tcagatagcg gaaattgagt tgcggcgtaa
     4381 agcccttcca agagattttg tcgtgccgca acgacaggtt tgcctgatat tcgtggtctt
     4441 tgcgcgggaa gcggtacaca atatgtcccg ggtgcatcaa aagtcctgcg ggtatagcgc
     4501 aggtttgccc gcagccccaa gccgccgtcg aacgttttga ccgcgccgac acgcaaaccc
     4561 ttgcggacgg aagcctgttc cgcctctttc gtcatgtcgt gcggccagtc cgcaccgccg
     4621 taaagcagcc ggtctttcgg cgcgggatac atcagcgtcg cgcccgccag cggcatatgg
     4681 ctgccgtatc gggcggcggt gcggtcttcc tgataatgct tccacatatt gcccgcgttc
     4741 agcgtcagcc gccagcgttc gcccaagcgt cgggagaaat cggcattgaa gccgccggcg
     4801 aaattgtatc ggctgccgcc caagaggttt tgcccgacaa acggcacggt gccgaacgag
     4861 cgcgttaccg aacggttttg atggccgaac gacaggcgca ggctctgttc gctgaaatct
     4921 ttgttatccc aataatgcac gccgcggctg atgccgccgt agaggaaatg atgcccgccc
     4981 gcattgattt cgcgcgacac gcccaagccg tagcgcaaac cgtgtgcctt ttgcggcagg
     5041 ctgtcggcgg ttttcgtcca gcttctgccc gcaaattcaa tcgttttttc ggacgaagcg
     5101 ttgtttatag cggattaaca aaaatcagga caaggcggcg ggccgcaggc agtacggatg
     5161 gtacggaacc gat
//
LOCUS       DAXFZR010000116         4911 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000116 DAXFZR010000000
VERSION     DAXFZR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.117"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..286
                     /locus_tag="ACFJAS_002014"
     CDS             <1..286
                     /locus_tag="ACFJAS_002014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983173.1"
                     /translation="SVLPTMADASNVSLDISNPTALRMGLPLYIQNLCSLILLNFTNK
                     KTARLFSSSNFKFVVCSGRTVSAKGSYSFAVSDFIWVAGFGKDCCCDPNV"
     gene            complement(599..958)
                     /locus_tag="ACFJAS_002015"
     CDS             complement(599..958)
                     /locus_tag="ACFJAS_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983174.1"
                     /translation="MIHKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RLARKDSLFEVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
     gene            complement(1079..2164)
                     /locus_tag="ACFJAS_002016"
     CDS             complement(1079..2164)
                     /locus_tag="ACFJAS_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC3983175.1"
                     /translation="MIYLFTGNMGAGKTPRVVSMILNNEDGLFKMKLEDGTEADRPLY
                     FCHIDGLDKRKFNARELAEGQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTRHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLD
                     NPAGVSGVEAANWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAVVGFVGWKSYGMF
                     QVYSKATDSRIEQEAQKESVVQTMTEQTASSETAPFEHSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DERQRTEQAAQSAKADKPQVLVMGGKS"
     gene            complement(2174..2464)
                     /locus_tag="ACFJAS_002017"
     CDS             complement(2174..2464)
                     /locus_tag="ACFJAS_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC3983176.1"
                     /translation="MPLLSGLIPLLGILLKMLIVRIILATGLTFVTYAGYLAALEKFK
                     GYTANAINSMPSDILNLLLISGFGQGLGCLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(2465..4030)
                     /locus_tag="ACFJAS_002018"
     CDS             complement(2465..4030)
                     /locus_tag="ACFJAS_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC3983177.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLEKSQIKFQSSNNLKSI
                     GFKLDSSSNSFSKFTEAANFEHIPTGAKARINAKITASVSRAGVLSGVGKLVRQGAKF
                     GTRAVPYVGTALLAHDVYETFKEDIKARGCRYDPETDKFVKGYEYSNCIWDFVENGIK
                     TYGCYGVDSSIMRLEKDYERFPEVKQLMESQMERIARPFWDKRKEEIPKDTALKLHLS
                     TFSHCEFFWNGGGCFVRNGDNLIHRVYFKLGRNPKYKEEMDAKKPEEILSLKVDADPD
                     KYIEATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVAATFGRDSQGNTTADVQVIP
                     RPDLTPASAQAPHAQPPPEVSPAENPANNPDPDENPGTRPNPEPDPDLNPDANPDTDG
                     QPGTSPDSPAVPDRPNGRHRKERKEGEDGGLSCDYFPEILACQEMGKPSDRMFHDISI
                     PQVTDDKTWFSHNFLPSNGVCPQPKTFHVFGRQYRASYEPLCVFAEKIRFAVLLAFII
                     MSAFVVFGSLGGE"
     gene            complement(3972..4289)
                     /locus_tag="ACFJAS_002019"
     CDS             complement(3972..4289)
                     /locus_tag="ACFJAS_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC3983178.1"
                     /translation="MNKPFITQAQLALYKYQPSSEYFGQSMAFIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNS
                     MFE"
     gene            complement(4417..4695)
                     /locus_tag="ACFJAS_002020"
     CDS             complement(4417..4695)
                     /locus_tag="ACFJAS_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983179.1"
                     /translation="MYCQVGNKCLEKHRAENLYFSLVVPRIQENGQIIRPEYNGSMWK
                     MSDGQPLRLSLAECSPKDNLQSGLETGRIVFGVLASVYFVSLLKKVLK"
     gene            complement(4702..>4911)
                     /locus_tag="ACFJAS_002021"
     CDS             complement(4702..>4911)
                     /locus_tag="ACFJAS_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC3983180.1"
                     /translation="INTCRKYGAKLAVVTAAPLALAAQANAALPETAKNALEAAKADG
                     MEAGWIVVGVFAALFVFSIVKRVMK"
BASE COUNT         1190 a         1172 c         1098 g         1451 t
ORIGIN      
        1 ctccgttttg ccgaccatgg cggatgcgtc aaatgtcagc ttggatatat ccaatcctac
       61 ggcgttacgc atgggattac ccttatatat tcagaatctg tgttctttga tactactcaa
      121 tttcacaaac aagaaaaccg cccgcctatt ctcgtcatca aactttaagt ttgtggtttg
      181 ttcaggccgg acggtttcgg caaagggtag ctattccttt gccgtgtctg attttatttg
      241 ggttgcaggt tttggtaaag attgctgttg cgacccgaat gtctgatttt ttttgggcgt
      301 atctcagtcc ggaatcactc cgttagtggg tttgcggtat tgaaaaacag ttcataaaaa
      361 aggaaaaggg gggtattcgt aaagattggg taacgcaccc aatctttaca aagcttcccc
      421 cttttccttt tttccgccct attttcctgc acgtacaacc cccgaacgaa gcgattccgg
      481 actgagacac tttaaaaaaa acagccattc tagcagttaa cccccttcgc tccgcccaag
      541 ccatcctgag gggtagtggc tgaatttgtg attttggttt tatcagattt tggttttatc
      601 aaacaaaata tttgactgaa gtcacatggc ggtcgtcata tgggggttcc ttcgcaccca
      661 aaaaatcgac cgcgtaacgg tttactaaaa cttcatcgtc cttaaacttt tggtgctttt
      721 tccggcaata ttttctgaac tccgttaaat ttgacggcaa gaacccgcaa ccgtctgcac
      781 cgtaaatgta atcaacttca aacaaactgt ctttccgggc aagccgacca tctttaagcc
      841 agaacatagg ctcgaaataa aaaactttcg acgtgtccgg gaacttatgg agacggagaa
      901 cacgtgtgaa atccccgttt tcatctacaa ttttgatata tcttggtttg tgaatcatga
      961 catcctcaga tttagtattc agaatatgat tttaaaaaga actttctgct ttacgactcc
     1021 gccgccgatt ccttcaaacg gttttccgcg ctcttcagtt gtcgtacatt aaattttatt
     1081 aggactttcc gcccattacg agaacttggg gcttgtccgc tttcgcggac tgtgccgcct
     1141 gttccgtcct ttgccgttcg tccttgtaag gattgaaagg caacccgttt ttcacatatt
     1201 ctttacacat tatctttgtt atttctttca agggtgttcc ttgatttgaa tagcatgtgc
     1261 aatctgattt tccgccgtct atgcatccgg cgatttgctc aaaggttttt acttgtcgga
     1321 ctgtgttata aataggcttg ctttcgggct tttcgggcaa agtcggcaca aagtcttcag
     1381 gtttcagatt gtcggaatgc tcaaaaggcg ctgtttctga tgatgccgtc tgctccgtca
     1441 tcgtctgcac aacgctttct ttttgcgctt cctgctcaat ccggctgtct gtggctttgc
     1501 tgtaaacttg aaacatgccg taacttttcc agcctacaaa ccctacaacc gcaatcaacg
     1561 cccaaaccgc ccaaggcact tttttcttga acttttggtg ttggcttgcg gatttatagt
     1621 atttgaaggc ttctttaggc ggtttccaat ttgcggcttc tacgccgctc acgcctgccg
     1681 gattgtccaa cgaggttacg catttatacc aataatactg tttcatgccg attgccttgc
     1741 gttcaaggtg tacatgcttt gaaacaaggt tgcggacgaa tatatcaagt tggctcgggt
     1801 gccgcgtcat caaaatgacg gtatgcccgt gatggcggag ttctgtcagt tcctgaatat
     1861 agggcggaac gggacggcct gccgcgcgta ccgggtaagt gtagtgcgct tcgtcaacaa
     1921 tcagcaccgc gccttccggt atgacatcac gaagcggggc ggacatgatt tgcccttccg
     1981 ccagttcgcg ggcattgaat tttcgtttgt ccaatccgtc gatatggcag aaataaagcg
     2041 gccggtctgc ctccgtgccg tcttccaatt tcattttgaa caatccgtct tcgttgttca
     2101 aaatcataga gacgacgcgg ggggttttgc ctgcccccat gtttcccgta aacagataaa
     2161 tcatgtttct acctcatccc ggaaagacaa acgtcagttt tttgaatgcg tgcataccaa
     2221 tgaagaacga gaatgcgccg aacaggcagc ccaacccctg accgaatccc gaaattaaaa
     2281 gaaggttcaa tatgtcggaa ggcatggaat tgatcgcatt tgccgtgtag cctttgaact
     2341 tttccagtgc ggcgagatac ccggcatagg ttacgaatgt cagacctgtt gcaaggatga
     2401 ttctgacaat cagcattttc agaagtatgc ctaaaagtgg aatcaggccg gaaagtaatg
     2461 gcatttattc cccccccaac gaaccgaaaa cgacaaaagc cgacataatg ataaaggcga
     2521 gcagtacggc aaaacggatt ttttcggcaa acacgcacaa cggttcatag cttgcccgat
     2581 attgcctgcc gaaaacatga aaggttttcg gctgcggaca tacgccgtta gacggtaaaa
     2641 agttatgtga aaaccatgtt ttatcgtctg taacctgcgg tatgcttata tcgtgaaaca
     2701 tgcggtccga aggtttgccc atctcctgac aggctaggat ttccggaaaa taatcgcacg
     2761 aaagcccgcc gtcttcgcct tctttccttt ctttgcgatg cctgccgttt gggcggtccg
     2821 gaacggccgg ggaatcgggg cttgttccgg gctgtccgtc cgtatcggga tttgcatcgg
     2881 gattcaaatc ggggtcgggt tcgggattgg gacgcgtgcc ggggttctca tcggggtccg
     2941 ggttgtttgc ggggttttcc gcgggcgata cttcgggcgg cggctgtgcg tgaggtgctt
     3001 gcgcgcttgc gggcgtgagg tcgggacgcg ggattacttg aacatccgcc gtggtgttgc
     3061 cttgcgaatc cctgccgaat gttgcggcaa cttgaacggg attcccgttc ctgtccgtga
     3121 cgggccccat attcactttt gttccgggtg cgacttctac tttttcggaa taaccgggat
     3181 atccggttgc ctctatgtat ttgtcgggat cggcatcgac tttcaacgat aaaatctctt
     3241 ccggcttttt ggcatccatt tcttctttgt atttcggatt gcggccaagt ttaaaataaa
     3301 ctcgatgaat taaattatca ccgttacgta caaaacaacc gccgccgttc caaaaaaatt
     3361 cacaatgact aaaagtagaa agatgcaact ttaaagcagt atctttagga atttcttctt
     3421 tccgtttatc ccaaaaagga cgagcaatcc tttccatttg actttccatc agttgtttga
     3481 cttcggggaa tctttcgtaa tctttttcaa gccgcataat agaactgtca acgccataac
     3541 agccataggt tttaataccg ttttctacga agtcccaaat gcaattacta tattcgtagc
     3601 cttttacaaa tttgtcggtt tcgggatcgt atcggcagcc tcgtgccttt atgtcttctt
     3661 tgaaagtttc gtatacgtcg tgggctaaaa gggctgttcc gacataggga accgcccttg
     3721 tgccgaattt cgcgccttgg cggacaagtt tgccgacccc cgacaatacg ccggcgcggg
     3781 atacgctggc ggttattttg gcgttgattc gggcttttgc gcccgtgggg atgtgctcga
     3841 aatttgccgc ttctgtgaat ttggaaaaac tgttagaact tgaatctaat ttaaaaccaa
     3901 ttgattttaa attattactt gattgaaatt taatttgtga tttttctaat cttacaggtt
     3961 cagaataaga attactcgaa catagaatta aaatcagaac tatcggtgta ctcttgataa
     4021 acgaattcat gaacagtttt tccgttctct tctgattttc ttataaaaat aacagactca
     4081 tcagaaaaat aaatcttcca aatattatgt gcgaccctct tatttaagaa ataagagaag
     4141 ctctctaaaa tatcgtactc ttttacgtta ttaacaaatt cttcaaattc tttctgagca
     4201 ataaaagcca tcgattgccc aaaatactcg cttgacggct gatatttata aagtgccaac
     4261 tgcgcctgcg tgataaacgg cttgttcatg gttctgcctt tcaaaggttg ttttgaaagc
     4321 ctgattttga caccataact tcatgcgctc aatccttaaa cagaaccgcc ccgattaata
     4381 cggggacggc aacgccgaga tagaaataaa aatccatcat ttcaaaacct ttttcagcag
     4441 ggaaacaaag taaacggacg cgaggacgcc gaatactatc cggcctgttt caagaccgct
     4501 ttgcaggttg tctttcggac tgcattccgc caatgaaagc cttagcggct gaccgtccga
     4561 catcttccac atgctgccgt tatattccgg cctgattatc tgtccgtttt cttggattct
     4621 tggtactacc aagctgaaat aaaggttttc ggcccggtgc ttctcaagac atttatttcc
     4681 gacttggcag tacatgccgc cttacttcat caccctctta acgatggaaa atacaaaaag
     4741 cgcggcgaaa acgcccacta caatccaacc ggcttccata ccgtccgctt ttgcggcttc
     4801 caaagcgttt tttgccgttt cgggcaacgc tgcgtttgcc tgtgccgcca aagccagcgg
     4861 ggcggctgtt acaacagcca gttttgcgcc gtatttacgg caggtgttaa t
//
LOCUS       DAXFZR010000117         4896 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000117 DAXFZR010000000
VERSION     DAXFZR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.118"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(1..215)
                     /locus_tag="ACFJAS_002022"
                     /pseudo
     CDS             complement(1..215)
                     /locus_tag="ACFJAS_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            887..1291
                     /locus_tag="ACFJAS_002023"
     CDS             887..1291
                     /locus_tag="ACFJAS_002023"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC3983181.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            1331..2512
                     /locus_tag="ACFJAS_002024"
     CDS             1331..2512
                     /locus_tag="ACFJAS_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC3983182.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(2615..4870)
                     /locus_tag="ACFJAS_002025"
     CDS             complement(2615..4870)
                     /locus_tag="ACFJAS_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC3983183.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
BASE COUNT         1193 a         1368 c         1280 g         1055 t
ORIGIN      
        1 aagaggaacg cctaacaggt ttcctttacc gtccgttatg tacgtttcgt aacattcgaa
       61 gacttcctga accctgcccg ccgtttcggt ttccgtaccc ccctgttaga taaggggggg
      121 ggaagattcg aagcggttgc cggcttcctg ccgtccgccg gcgcgtccgt catcacgccg
      181 gcaaccgcct ttgtcatccc ttgcttatct cccatttgcg aatcctcaaa aacgggcaaa
      241 aaaaaagccc tgttacttgt agaaagtaaa gggcgttaat ttttgttaat cgccccttct
      301 tagggacgca atatataagg tttcagacgg cattgttctc ccgccgtcat tcccgcgcag
      361 gcgggaatcc ggaacgtaaa atctaaagaa accgttttat ccgataagtt tccgtgccga
      421 caggcccgga ttcccgcctg cgcgggaatg acgggatttt aggtttctga ttttggtttt
      481 ctgtttttga ggaaatgacg ggattttagt aaccgtaaca actgcctgcg cgacggctaa
      541 ggggcttcag caaccgtagc aactgcctgt gtgggaatga cggacaatgg gcttcagacg
      601 gcatctcttg ccttccgcta aaacagtttg ccgcacaact gttcaaacgc gtccgatatg
      661 tttcaacaca caggacgaca cataaagcac cgccctatat gtcgtcctga ttcggaaggg
      721 gttacgcccc tcccaaacaa agtctgatcc tgccgcccta aagggcgggg tttcaaccga
      781 aaaggaaaca cgatgaagtg gtacaattaa cggcaatgcg gacagacaaa ttaaactatc
      841 agaaattgcc ctaccgtttt ttaaacactt tcaggaataa ggaaaaatga ccgcccaacc
      901 ctgccccatc tgcacggcac aagatgaaga tattttgctg caaaccccca agctgcgcgt
      961 catcgccgtc cataacgaca gcggttcgcc ggcattctgc cgcgtcattt ggcacggaca
     1021 cattgccgaa attaccgacc tttcggcagc ggaacgcggc gaattgatgg aaatggtgta
     1081 caaagtcgaa gccgctatgc gccaagtgtt ccggccggca aaaatcaacc tcgccagctt
     1141 gggcaatgtc gtgccgcacc tgcattggca tattatcgcc cgctttgaaa acgatgcgac
     1201 tttccctgcg ccgatttggg caaaccccgt ccgaaaacac ggtatgaccc tgccgcaaaa
     1261 ttggacggaa cagcttaaaa agctgcttta aacccgccga tgccgtctga aaccgtatga
     1321 aagggaaatt atgaccgaac cgacctcccg ccgccgtttt ctgaaaacct gcaccgccgc
     1381 cggcgcgggg ctgcttcagg cttgcggcac atccgccaca tccgttccgc cccttccctc
     1441 ttcccattcc gttgtgaaag cccgaaccgt gcctctccaa acgccacgcc gtcaaagttc
     1501 ggacggcaac cttctgcgcg ttgtcgcttc gtcaggattt gccgaagaca ccaaccgcgt
     1561 caacacagcc ttaacccgcc tttacaatgc cggttttacc gtaaccaacc aacaggcggg
     1621 cagccgccgt ttccaacggt ttgccggcac ggacgcgcaa cgcgcggcag atttccaaga
     1681 ggtcgcctcc ggccgcgtcg ccacgcctaa agtgctgatg ggcttgcgcg gcggttacgg
     1741 tgcggcgcgg attctgccgc atatcgattt tgcttcgctc ggcgcaagga tgcgcgaaca
     1801 cggcacgctg tttttcggat tcagcgacgt atgcgccgtc caactggcat tgttggcaaa
     1861 aggcaatatg atgagttttg ccggcccgat ggcttatagc gattttggca aacctgcccc
     1921 cggtgcgttt acgatggatg cctttatcaa gggggcaacc caaaaccgcc tgaccgttga
     1981 tgttccttat atccaacgcg ccgatgtcga aaccgaaggc acgttgtggg gcggcaactt
     2041 aagcgtcctc gcctcgctcg ccggtacgcc ttatatgccc gacatcgacg gcggcatttt
     2101 gttcctcgaa gatgtcggcg aacagcccta ccgcatcgaa cgtatgctca atacgctgta
     2161 tctttcgggt attttgggca aacagcgcgc catcgtgttc ggcgatttcc gtatggaaaa
     2221 aattcgagat ctctatgatt cgtcttatga tttttccgcc gttgccaagc atatttcgcg
     2281 cacggcgaaa atccccgtgc tgacgggctt cccgttcgga cacattgccg acaaaatcac
     2341 tttccctctg ggcgcgcaca cccgaatccg tatgaacgga aacggcggct attcggtcgc
     2401 gtttgaaggc taccccacac tcgatgcgtc cgccctgact ttggataccc tgctcccacc
     2461 gccggatttg cctatcttcc ccgaaagcgg tgttgccgat atttcggaat aaacccgcaa
     2521 acggacaaat gccgtctgaa gccttcagac ggcattttcc tgtaaaaaaa accgcacctg
     2581 atggcaggtg cggtttccat tttcaggcta aaacttattt gtcgcggccg aatacgattt
     2641 tagtggcttg gatggcaacg cagattgcac cgccgataaa gattaagtcg ggggcagtgc
     2701 gtacccagcg cagggtgtcg aggatttcca tttgcaggaa ttcttcgctg cgggcatacc
     2761 acaaaccgtg cgtgatagag gcgtatgcct gaatcacgcc gacaggcagc aggctgatgg
     2821 caatcatacc gaccaagccg ccgttgagca gccagaagcc ccaagtcatc agtttgtcgt
     2881 caaaccgcgc gttcggtttc aagtagcgcg caaccaacaa tacgaagccc aatgccaaga
     2941 aaccgtacac accgaacaag gcggcgtgcg cgtgaacggc ggaagtgttc aaaccttgga
     3001 tatagaacag ggaaatcggc ggattgatca gaaagccgaa tacgccggca ccgatcatat
     3061 tccaaaaagc gactgccacg aagcacatca gcggccaacg caggcgtttc gcccagtcgg
     3121 acaggtgttg gtaagaccag tgctcgtatg cctcgcggcc cagcaacacc agcggcacga
     3181 cttccaaagc ggagaagcag gcgccgattg ccatagaggc ggaggtagag ccggagaagt
     3241 acaggtggtg cagcgtgccc ggaacgccgc ccaacataaa gatggcggca gcggccaaag
     3301 tggaggcagt ggcggtactg cggcggacaa agcccatatt gtagaagaca aaggcaaagg
     3361 cggcagtggc aaatacttca aagaagcctt ccacccacag gtggaccacc caccaacgcc
     3421 agtattccat tacggcaatc ggggattttt cgccatagaa caggcccggt gcgtagaaca
     3481 cgcccacgcc gaccatagaa gcgacaaaga ttgccagcag gtttttgtcc acgccttttt
     3541 ctttgaaggc ggaaaccgtg cagcgcaaca tcaggaacag ccacaacagc agtccgacca
     3601 tcaaaaggag ttgccaaaaa cggcccaaat cgaggtattc gtaaccttgg tgtccgaacc
     3661 agaagttaaa ttcgggagga aggatgtgcg tcaacgcgaa gaagttgccc gcgtaagaac
     3721 cgccgaccac gatgaagagg gcgatataga ggaagttcac gcctgcacgt tggaacttgg
     3781 gatctttgcc gccgttgaca atcggcgcga ggaacaaacc tgccgtcaaa aagccggttg
     3841 caatccagaa gatggcggat tggatgtgcc aagtacgggt cagggcgtag gggaaccagt
     3901 cggacatttc aaagcccaac gcctcgtcaa tgccgtagaa accctgacct tcgacggtgt
     3961 agtgcgcggt caggccgccc agcaatactt gtaccacaaa cagggcgacc gtcaggaaga
     4021 cgtatttgcc caatgctttt tgcgaagggg tcagttggat tttggaaatc gggtcttcag
     4081 acggcacttc cacttcctcg tgtttggtca ggaaggaata accccacatc aacaaaccga
     4141 tgcccatcaa gagcagtacg acactggtaa acgaccacat gtagttttca gtagtcggta
     4201 cattgttgat caaaggctcg tgcggccagt tgttggtgta ggtaaagacc tcgccgggac
     4261 ggttggtcga agcagaccaa gaagtccaga agaagaagtc gaacagtttt tcacgcgctt
     4321 cttggcttgg caatgtgttg tttttcattg caaagtgttc gcgggtggtt tgcaacttgg
     4381 gatcatcgcc gtaaacgccg tgatagtaag gcaggatgct ttcgatggct ttcacgcgcg
     4441 tatcgctgat gacgacgctg ccgtcttctt taatacggct ttgattgcgg tattcatcgg
     4501 ccaagcgggt tttcagaacg gcttgttctt caggggaaac ttcatcgaat tttttgccgt
     4561 aagcctgttg cgcggtcaaa tccaaccagg cggacaactc acgatgcagc cagtcggccg
     4621 tccaatccgg agcctgatat gcgccgtgtc ccagaatcga accgacttcc atgccgccgg
     4681 tagtctgcca cgcagactga cctgccaaaa tatcgtcttt cgtcatcagc actttgcctg
     4741 atgcggaaac gacctgttcg gggtaaggcg gggctttctt ataaacctcg ctgcccatat
     4801 agccaagaat ggtaaagcat accgccagaa cggcaaacag caagtaccac agcttcttgt
     4861 actgtcccat tttgagagct ccttttaata aatcag
//
LOCUS       DAXFZR010000118         4695 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000118 DAXFZR010000000
VERSION     DAXFZR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.119"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(180..3011)
                     /gene="lbpA"
                     /locus_tag="ACFJAS_002026"
                     /gene_synonym="tbpA"
     CDS             complement(180..3011)
                     /gene="lbpA"
                     /locus_tag="ACFJAS_002026"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC3983184.1"
                     /translation="MNKKHGFPLTLTALAIVTAFPAYAAQAGAAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSRGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQYNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3008..>4695)
                     /locus_tag="ACFJAS_002027"
     CDS             complement(3008..>4695)
                     /locus_tag="ACFJAS_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC3983185.1"
                     /translation="THRFGYDGFVYYSGERPSQSLPSAGTVKYFGNWQYMTDAKRHRT
                     GKAVASDDLGYITFYGNDIGATSYAAKDADNREKHPAEYTVDFDKKILKGELIKNQYV
                     QKRNDPKEPKKPLTIYNITADLNGNRFTGSAKVNTEVKTRHADKEYLFFHTDADQRLE
                     GGFFGDNGEELAGRFISNDNGVFGVFAGKQKTNASGTNPAMPFGKHTKILDSLKISVD
                     EATDENPRPFEVSTMPDFGHPDKLLVEGREIPLVSKEKTIDLADGRKMTVSACCDFLT
                     YVKLGRIKTERPAVKPKAQDEEDSGINNGEESEDEEIAEESEDEVSEDDNGEDEDEIV
                     EEEADEAEEIEEEAEEEEPEEESPEEGNGVSDGIPPAPEALKGRDIDLFLKGIRTAEA
                     DIPKTGTAHYTGTWEARIGEPIQWDNKADKAAKAEFDVDFGNKSISGTLTEQNGVEPA
                     FRIENGVIEGNGFHATARTRDNGINLSGNGSTNPQSFKADNLLVTGGFYGPQAAELGG
                     TIFNKDGKSLGITEDIENEVENEADVGEQLEPEVKPQFGVVFGAKKDNKEVEK"
BASE COUNT          967 a         1276 c         1114 g         1338 t
ORIGIN      
        1 tttttgagtt tcagtcattt ccgataaatt gccttagcat tgaatgtccg gattcccgcc
       61 tgcgcgggaa tgacggcgga aatcttgttt atattgaatc aaaaaaacct gcaccttaat
      121 cagttggcgg tttagtccga cttttggggt gcagatcaag cttccagacg gcatttcctt
      181 taaaacttca tttcgagcgc gaggctgaag ttcctgcccg gtgcggcata ccttccatag
      241 ttgctgtcgc cgccgtgccg gtttgccgtg ctttccgcag tctggcgcaa ggattcccaa
      301 gtaacgtagc ggtagttgcc gatattgtag atagccgccc tcaaggtcag ccgttttttc
      361 agattcagat aagcggaaac atctgccgtt ttccaagaag acgtaaccct tcctgccgaa
      421 tatcgttttt gatcgcctgc cagataagca agctcgtcag ggtttttccc tttggaatag
      481 gtcagcataa tgtttgcgcc ccatttcccc tcgggctggt cgtatccgaa ccccaaaaca
      541 taacgcgacg gctgtaccgc atccaaagca tagctgcgga gggacaagtc cggccggttg
      601 gataccgatt tcggtttgat acggttgtac gccaatgtgg tgtacaggcc ttcgggcagt
      661 ttgccgtata cgccgttcca gtcgattttc cccaagatgt tgatgccttg aagcgacata
      721 ttttgggcat tgtaataatc gcgtatatca atctctgtca atcgtcctgc tgaatccggc
      781 aatttggttt tttgatcggc aacggcaatc atatcggtat aacggttgcg gaaactgctg
      841 atttccaaaa agccgaaatc gcccttccac tgcaaaccga tttcccggtt ggctgccttt
      901 tccgatttca gggcgggacg ctgccagcct ttcggataat cgtgataaat gtctatcccg
      961 aaaagttctt ggaaggaagg cgttctgaag ccgctggagg cgcggtaaga cacggaaaaa
     1021 tgccggttcg gtttgaacac gatgccgctg ttccacgaac ggtcggcata ccgcccgctg
     1081 cggacgagtt cttccgacgt ggtgaagttt ttccggtcgt acctgccgcc caagctgaaa
     1141 tcaaaatatt tgccgattga aaaacggtcg ttcaaagaaa tgtggatatt gctgccgttg
     1201 atttttcttg gcacgcattt gcgggaacgc agggtttcga tatagccgca gaccgaccct
     1261 tcgacgactt cgggcttacc caaacggtac ttatcttgat tcttttcatc gaatcccgtg
     1321 gattccgaaa tccttgccgc attgtgggaa agctgttctg ggcgggagat cgctttggaa
     1381 gcatcgtaac cgaagcccaa agtcagatgg tgtttcgtcc atttgttttt cagcgatttc
     1441 tcaaacgagg cattcaaaac attgtactgt tcgcggtagt ggaaacggtc gctgctgtcg
     1501 taggaatacg gtttgtccgc cgacgcgcgg caggatttgt ccacggcagg atacacggcg
     1561 caattcagct tcagcgtgtt gttgtcggtt gccacgccct gtttgtcaaa cgacaacacc
     1621 gccttatccg cccagttgtc cgagtatttc tcattttcat aacggtacag caaacccata
     1681 cggcggcggc ggtgatgttc gtcaataaat ttggtgcggg aatatttcaa acctatgccc
     1741 ttgaccaaat ttttatcgcc cttccactct tctatattcg gcacaaaata caagccgtcg
     1801 cggaaatcgt cgccgtcgta caccccgctc ttgtctctaa acttttccgc ctcgtccgta
     1861 ccgtaatact gtttttccgt catatcgcgg atgtcgtaac gctgtttggt atcctcaaac
     1921 acgccgccga cataatgcct gccgccgaag cggtagccca gcttggcaag ccaagagccg
     1981 ctgcggtaat ccatcggatc gggcaatatc ctgccgccgc ccgtgtaggc ttgggcggac
     2041 aggttttcgt ggcgcgcctg cgcctcccgc acctgcgcct cttcttcagc acttaaaggc
     2101 tgattttgtt caatacgttc ttttacccag cggttgagct ggcggttcaa atatatcccg
     2161 tagcccgcca attttgccac gggcttgagt gtattctcgc cctctactaa gaaaaatggc
     2221 tctgttgtct tgcgtttaat atcgtatgtc tggcggaacg cgtccaaacg gtctatgccg
     2281 tattccaccc cgtccgcaat atcgccgtgc gggcgcgttt cccgcccttg gcgttcggtt
     2341 cggattagca gcccttccca accgtctttg ctgaaccccg cgccgagcga cttcataaat
     2401 tggcggtttt tactgccgta agcggttttt gcctgtatcc cccggctttt gccgtctgaa
     2461 atcaggtctg ccgcctcttt ggtgcggaag gcgaccgcgc cgccgagtgc gccgctgccg
     2521 tgatcggacg aaccggcgcc tttgtcgatt tccaccgtgc tgatgttttc atattcgatt
     2581 tcgttgattg cgccgctgcc gccgcgtccg ccgtatccgc tcaacgatcc ctgcacggta
     2641 aacgcctgta tttgggcaac gccgtcaacc gaaaccgcca cacggttttt atctacgccg
     2701 cgtatcgagt agccgccgct cgcgccgttg ccctgttcga caaccgccac gcccggatcg
     2761 tagcgcgtca ggtcgcggat accgagtacc tgttctttgt tcaacgtttc cgacgttttg
     2821 acgattttgc ccaaacctgt cgcctctttc gatcgccgtc ccactttggc ggcacggacg
     2881 gtaacctctt tcaatgattg actttgcgcc gcatcaagtg ccgccgcccc cgcttgggca
     2941 gcataagccg gaaaagcggt tacaatggcc aacgccgtca aagtcagcgg aaaaccgtgt
     3001 ttcttattca tttttccacc tctttattat ctttcttcgc accgaatacc acgccgaatt
     3061 ggggtttaac ttcaggttct aactgttcgc caacatcagc ttcattttca acttcatttt
     3121 caatatcttc agttatacca agagatttcc catccttatt gaaaatagtg ccgcccaatt
     3181 ccgccgcctg cgggccgtaa aagccgcccg ttacaagaag attgtcggct ttgaaacttt
     3241 gaggattagt cgaaccattt cccgaaagat tgatgccgtt atcccgagtg cgtgctgttg
     3301 cgtggaaacc gttgccctca atcacgccgt tttcaatacg gaaagcaggt tctacaccgt
     3361 tttgctccgt cagcgttccg gaaatcgatt tattgccgaa atcaacgtca aattctgctt
     3421 ttgccgcttt atccgcctta ttatcccatt gaatgggttc gccgatacgc gcttcccaag
     3481 tgccggtata atgcgccgtt ccggtttttg gaatgtcggc ttccgccgtg cggatacctt
     3541 tcaggaaaag gtcgatgtcc ctgcctttaa gggcttccgg ggcgggcggg atgccgtctg
     3601 aaacaccgtt gccttcttcc ggcgattctt cttcgggttc ttcctcttca gcttcttctt
     3661 cgatttcttc agcttcatca gcttcttctt cgacgatttc gtcttcgtct tcgccgttat
     3721 catcttcgga aacttcgtct tcgctttctt cggcgatttc ttcgtcttcg ctttcttcac
     3781 cgttattaat acccgaatcc tcttcgtcct gcgccttcgg tttgacggcg gggcgttcgg
     3841 tttttatccg tccgagtttc acataggtca aaaagtcgca acaagcactg acggtcattt
     3901 tcctgccgtc ggcaagatcg atggttttct ctttgctaac caaaggaatt tcacgccctt
     3961 cgacaagaag tttgtcagga tgaccaaaat cgggcatagt ggaaacctca aacggacggg
     4021 gattttcatc agtcgcctcg tcaacggaaa tttttagaga atccaagatt ttggtgtgtt
     4081 ttccaaacgg catagcagga tttgtgcctg atgcgtttgt tttttgtttg cctgcgaata
     4141 cgccgaatac gccgttgtcg ttactgataa accgtccggc aagctcttct ccgttatcgc
     4201 cgaaaaaacc gccctcaagc cgctgatcgg catcggtatg gaaaaacaaa tattctttat
     4261 cagcgtgtct cgtcttcacc tcggtgttaa ctttggcact gccggtaaag cggttgccgt
     4321 ttaaatcggc agtgatgttg taaatggtca gcggtttttt aggttccttt ggatcatttc
     4381 tcttttgcac atactgattt ttaatcagct cgccctttag gatttttttg tcgaaatcaa
     4441 ccgtatattc ggcaggatgt ttttccctgt tgtcggcatc cttagccgca taagaagttg
     4501 caccaatatc gttaccataa aatgttatat aacccaaatc gtcactggca accgccttac
     4561 ctgtccgatg acgtttggca tcggtcatat attgccagtt gccgaaatat tttaccgttc
     4621 ccgcgctcgg taaagattgg gaaggacgtt ctccggaata atatacaaaa ccgtcataac
     4681 caaaacggtg ggtaa
//
LOCUS       DAXFZR010000119         4405 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000119 DAXFZR010000000
VERSION     DAXFZR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.120"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..1229)
                     /locus_tag="ACFJAS_002028"
     CDS             complement(<1..1229)
                     /locus_tag="ACFJAS_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983186.1"
                     /translation="MKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTLVLAK
                     QGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSIERNI
                     NKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSSSLAP
                     AMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDK
                     GQGITANHYGNSDINFNKASPNITTMEFKGGVNIKIDRNGQEEAESNGFGFYSSRKLG
                     NKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKV
                     VKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPD
                     TKSIEKKKLNVRGCTRLS"
     gene            1428..1503
                     /locus_tag="ACFJAS_002029"
     tRNA            1428..1503
                     /locus_tag="ACFJAS_002029"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1462..1464,aa:Glu,seq:ttc)
     gene            complement(1719..1871)
                     /locus_tag="ACFJAS_002030"
     CDS             complement(1719..1871)
                     /locus_tag="ACFJAS_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983187.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            1931..2737
                     /gene="thiD"
                     /locus_tag="ACFJAS_002031"
     CDS             1931..2737
                     /gene="thiD"
                     /locus_tag="ACFJAS_002031"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC3983188.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2795..3664)
                     /gene="tehB"
                     /locus_tag="ACFJAS_002032"
     CDS             complement(2795..3664)
                     /gene="tehB"
                     /locus_tag="ACFJAS_002032"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC3983189.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            3777..4214
                     /gene="rnhA"
                     /locus_tag="ACFJAS_002033"
     CDS             3777..4214
                     /gene="rnhA"
                     /locus_tag="ACFJAS_002033"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC3983190.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1018 a         1240 c         1042 g         1105 t
ORIGIN      
        1 gataatctag tacagcccct aacattcagc tttttctttt caatcgactt tgtatcgggg
       61 gttaattcat atccgtccgc atccacggtt aaggcaaaca tatctttccc tttggcaaag
      121 ctccttttcc cgatatgggc ttccccttca aaataagagt ctttgttggc aaggttcacg
      181 ctcacgctgc cgccgttgta ggcgaaaata tcgcctttga ttttgacaac cttgccttcc
      241 ccgccgacta cctcgacttt gctgtatttc ccgtcaatcg caaacgcgct gcctatgctt
      301 ttgggcctgc cttgctcgtc ataaaccggc gttgcaacga tatcgacgtt cccacggaaa
      361 atcgcttcca tcttagaacc ttccggaatc tgcttcttat tgcctaattt gcggcttgaa
      421 tagaagccaa aaccgttgct ttccgcctct tcctgtccgt tcctatcgat tttaatgttt
      481 acaccgccct tgaactccat tgtggtgatg tttggagaag ccttgttgaa gttgatgtct
      541 gagttaccat agtggttggc ggtaatgccc tgtcctttat cttttcctga tttgatgttg
      601 atattcagcg tcgagccttc ttcggaaaca agacgatatt ctcccatttt ttcaccatta
      661 ttaagcaata ggataccttc gttactagag ttggcatttg agtccgcgtc catattgatg
      721 gtattgcgcc ctttaaaggc aagcatcgcc ggcgccaagc tactggattt atgcctatca
      781 tcagacaaaa tgccggcagg cgcgttatgg gcggctatgg ttacgttttc aaaagtaacc
      841 ttattttctc cctcatagct ttgatccacc aacatcaccg cgccgctcca attctgccat
      901 gcgtacgtgt aaccgttttt atttatattt ctttcaatcg agttattgtc aatcgtcggc
      961 ggggcatcgg caggaccgtc tgccaatttg ccttttatcg taatgttgtt cgtaccgccg
     1021 ttggcggcaa tgatgaggga atttaagggt ttgacttcgg ttccgccatt aagggtatag
     1081 ttgttctctt gcccttgttt tgcgaggacg agggtgtatt tgtctgtctt ctctatcgtt
     1141 ccactgtttg cagcggccgc cccttgcgcg tgcatagaag cggccgcgaa gccaagtaag
     1201 atgcactgaa ctgtcttctt ttgtttcatc ggtttttcca ttttgaaggt atgtgatttt
     1261 tatcattata tgcttaaatc attgacacgc ccccaaaaaa aaaatgaatt gcttgatatt
     1321 ccaatgtttt ttatcttgtg atacggtcag acaaacgccc cctgacattc gtttagacgg
     1381 catcgtattg ctaaatttct ataagtatgt ataatgtccg tttccacgcg cccatcgtct
     1441 agaggcctag gacactgccc tttcacggcg gcaaccgggg ttcgaatccc cgtgggcgtg
     1501 ccaattcaaa aacctgcttg tttcaagcag gttttttatt atgaatcgtt attcccgcaa
     1561 tttttcgtca ttgccgtgaa aacgggaatc tagaacctca aactttcaga taatctttga
     1621 atattgctgt tgttctaagg tccggattcc cgcctgcgcg ggaatgacgg aattttaggt
     1681 ttctgttttt gcgggaatga cgaatgaagc gtgccggttt atactcgccg caacacgcgg
     1741 ttcagacggc atcgccctct tttttcatta tcagttgcta cgatgcaccc cgctgccttg
     1801 ccctgtgcct tgtcctgcaa tacgacataa taatgcacca caaaccccgc gctgcggttt
     1861 tcagacggca ttgcctgctt ttttacaggc agtagccctt tttatcggac gcaatattaa
     1921 ggaggaacaa atgaaaggct cttttgtgca gacgcttgcc atcgccggtt cggattcggg
     1981 cggcggtgcg ggcattcagg cggatttgaa aacgtttcag atgcgcggcg tgttcggaac
     2041 gtgtgtcatc accgccgtta ccgcgcaaaa taccttgggc gtgtcggcgg ttcatctcgt
     2101 cccgaccgaa accatcaccg cacaaatcca agcaatcagg gaagactttg acatccgcgc
     2161 ctacaaaatc ggtatgctcg gcacggcgga aatcatcgaa tgcgttgccg acaagctgaa
     2221 acactgcagc tttggcaggc gcgtactcga ccctgtgatg attgccaaag gcggtgcgcc
     2281 gctgttgcag gattccgccg ttgcggcact gacgcgcctg ctgctgccgg acacggacat
     2341 cctgaccccc aacctgcccg aggcggaagc cttgacgggc gtgcatatcg aaaaccgtaa
     2401 agatgcggaa cgtgcggcaa aaatcctgct tgattacggt gtcaaaaatg tcattatcaa
     2461 aggcggacat ttgaacggca gcacaagcgg acgctgcacg gattggctgt ttacgcaaaa
     2521 cgaaacgctg gaattggaca gcccgcgctt tccgaccgcc cacacgcacg gcacgggctg
     2581 cacgttttcc gcctgcatta ccgccgagtt ggcaaaaggc ttagacgttt gcaaagccgt
     2641 acagactgcc aaggcctaca tcacggcggc aatctcaaac cctttggaaa tcggcgcggg
     2701 acacgggccg gtcaatcatt gggcgtatcg ggactagacg taaaaatgcc gtctgaacgc
     2761 cttttcgggt tcagacggca tcgtccggcg cggcttatcc tggttttttc gccagcatag
     2821 ttacaaattt aaaccgtatc gggttgccgt tttcgtcttt ggcgtgcatc gcgcccaatt
     2881 cttctttata ttcgaccagc tcccaatccc gataataatc cttcagctcg ccctctttaa
     2941 atttgaaggg gaacggcatc gggcagggga aatccgccgt atccattgcc gatacgatta
     3001 agttgtaccc gcccgccgcc gtatgcgcct gcatatcggc aatcacgtcg ggcacgcgct
     3061 gcggcatcag gaacatcagc accaccgttg ccacaatata atcaaactcg ccctgaaggg
     3121 cggcggcgtt caaatcatat tccagcgtgc ggacgttcaa accctccgcc tctgccagtt
     3181 ccgccacgtt tgccaacgcg gcgggattgc aatcggcagc ggttacatca aaccccttca
     3241 aacccaggaa cagcgcgttg cgcccctgtc cgcagcccat atccaacgcc ctgcccgccg
     3301 gtacggtatc ctgtgccgcc gcgaccgcag aatgcgtggc actcatcccg tattttttgt
     3361 gaaaatagtc tgccgccgcg caatacagcg acaaacggat ttcggcatcg tccgttttcg
     3421 gtttgaccga aaacacctgc tgcggcgcaa acacgcaatc gccgctttcc gccgaccaaa
     3481 cctctgccgc cccgtccggt gcacggactt cgacatcgcc ctgcaacaca ttcaggcaga
     3541 cccattcccc ttcctcggac gaataacccg acaacaaaac ttccggcagg ttttccactt
     3601 tccatacagg catttgtccg aaacaaaaca actcgccact ttgacccact atccgctcct
     3661 tcatattcaa aaataaagtt gcacattata tgcctatttc aatccgccgc aatctttcag
     3721 acggcacgac gcacaaaccg cttataatca cgccggacac cacacaaagg cacgatatgg
     3781 acacacccgt ttacctctac acggacggcg cgtgcaaagg caatcccggc gcgggcggct
     3841 ggggcgtatt aatgcgctac ggcagccgcg aaaaagaact tttcggcggc gaagcgcaaa
     3901 ccaccaacaa ccgcatggag ctgaccgccg ttatcgaagg gctgaaatcg ctcaaacgcc
     3961 gctgcaccgt catcatctgc accgactcgc aatacgtcaa aaacggcatg gaaaactgga
     4021 tacacggttg gaagcgcaac ggctggaaaa ccgccgccaa acagcccgtc aaaaacgacg
     4081 acttgtggca agaactcgac gctctggtcg ggcagcatca agtcagctgg acttgggtta
     4141 aaggacacgc gggacacgcg gaaaacgaac gcgccgacga tttggcaaac cgtggcgcgg
     4201 cgcagttttc ttgattgccg caccgacaaa aatgccgtct gaaaccgcta atgggcttca
     4261 gacggcatcg tcctccaccg tcattcccgc gcaggcggga atccagaacg tcgggcaacg
     4321 gcaatattca aaagccgtct gaaaatttaa aagttctaga ttcccgtttt cacgggaatg
     4381 acgaaaagtt gcggacagca gagga
//
LOCUS       DAXFZR010000120         4169 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000120 DAXFZR010000000
VERSION     DAXFZR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.121"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..209
                     /locus_tag="ACFJAS_002034"
     CDS             <1..209
                     /locus_tag="ACFJAS_002034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983191.1"
                     /translation="NSKKKETGKIRHKTLTYSKPSVLQRKYPKKTICLPSVSNRYTAG
                     TRYFSDGAAVVRTDKNVTAAERIA"
     gene            263..2647
                     /gene="ppsA"
                     /locus_tag="ACFJAS_002035"
     CDS             263..2647
                     /gene="ppsA"
                     /locus_tag="ACFJAS_002035"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC3983192.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2868..4127
                     /gene="rho"
                     /locus_tag="ACFJAS_002036"
     CDS             2868..4127
                     /gene="rho"
                     /locus_tag="ACFJAS_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC3983193.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
BASE COUNT         1088 a         1240 c         1000 g          841 t
ORIGIN      
        1 aaaattccaa aaaaaaagaa acaggaaaaa tacggcataa aacattaacc tattcaaaac
       61 catctgtttt acaaaggaaa tacccgaaaa aaacaatatg cctgccatcg gtttcaaacc
      121 gctacacagc cggaacccgc tatttttcag acggcgcagc ggttgtccgg acagataaaa
      181 acgtgacagc ggcggaacga atcgcttaaa ataagcaagc ggatttgttt cttttttaac
      241 atattttgga ttggacacac aaatggccga caactacgta atctggtttg aaaacctgcg
      301 tatgacagat gttgaacgcg tgggcggtaa aaacgcctcg ctgggcgaaa tgatcagtca
      361 actgaccgaa aaaggcgttc gcgtccccgg tggctttgcc accacagccg atgcctaccg
      421 cgcatttctc gcacacaacg gtctgaacga acgcatttcc gccgctctgg caaaattgga
      481 tgtcgaagac gtttccgaac tggcacgcgt cggcaaagaa atccgccaat ggattttgga
      541 cacgcccttc cccgaacagc tcgatgccga aatcgaagcg gcatggaaca aaatggttac
      601 cgatgccgac ggcgcggaca tttccgttgc cgtacgctct tccgcaaccg ccgaagacct
      661 gcccgacgca tcatttgccg gccaacagga aaccttcctg aatatcaatg gcttggataa
      721 cgttaaaaaa gcgatgcgcc acgttttcgc ctccctgtac aacgaccgcg ccatttccta
      781 ccgcgtccac aaaggcttcg aacacgacat cgtcgccctt tccgccggcg ttcaacgcat
      841 ggtgcgttcc gacagcggcg catcaggtgt gatgttcacc ctcgacaccg aatccggcta
      901 caaccaagtc gtctttgtta cctcctctta cggtctgggc gaaaacgtcg tacaaggcgc
      961 ggtcaacccg gacgaatttt atgtgttcaa acccacgctc aaagcaggca aacccgccat
     1021 cctgcgcaag accatgggtt cgaaacacat caaaatgatt ttcaccgaca aagccgaagc
     1081 cggcaaatcg gtaaccaacg tcgaagtccc cgaagaagac cgcaaccgct tctccattac
     1141 cgacgaagaa atcaccgagt tggcacacta cgcgctgacc atcgaaaaac actacggccg
     1201 cccgatggat atcgaatggg gacgcgacgg cctggacggc aaactctaca tcctgcaagc
     1261 ccgccccgaa accgtcaaat cccaagaaga aggcagccgc aacctgcgcc gctacgccat
     1321 caacggcgaa aaaaccgtct tgtgcgaagg tcgtgccatc ggtcaaaaag tcggtcaggg
     1381 caaggtgcgc ctgattaaag atgcttccga gatggattcc gtcgaagccg gcgacgtact
     1441 ggttaccgac atgaccgatc cggattggga acccgtgatg aaacgtgctt ccgccatcgt
     1501 gaccaaccgc ggcggacgca cctgccacgc cgccatcatc gcgcgcgaac tgggtattcc
     1561 tgccgtcgtc ggctgcggca atgcgaccga actcttggaa aacggtcagg aagttaccgt
     1621 atcctgtgcc gaaggcgata ccggctttat ctatgccggc ctgctggacg tacaaattac
     1681 cgatgtcgcc ttggacaata tgccaaaagc acccgtcaaa gtcatgatga acgtcggcaa
     1741 tcccgaactc gcattcagct tcgccaatct gcccagcgaa ggcatcggct tggcgcgtat
     1801 ggaatttatc atcaaccgcc aaatcggcat ccacccgaaa gccttgttgg aatttgacaa
     1861 acaagacgac gaactgaaag cggaaattat ccgccgcatc gccggttacg catcccctgt
     1921 cgacttctac gtcgataaaa tcgccgaagg cgtggcaacg cttgccgcat cggtttatcc
     1981 gcgtaaaacc atcgtccgta tgtccgactt caaatccaac gaatacgcca acctggtcgg
     2041 cggcagcgta tacgaaccgc atgaagaaaa cccgatgttg ggcttccgtg gtgcggcgcg
     2101 ttatgttgcc gagagcttca aagactgttt cgccttggaa tgcaaagcct tgaaacgcgt
     2161 tcgtgacgaa atgggtctga ccaacgtcga aatcatgatt ccgttcgtcc gcactttggg
     2221 cgaagccgaa gcagtggtta aggcattgaa agaaaacggc ttggaacgcg gcaaaaacgg
     2281 cctgcgcctg attatgatgt gcgagctgcc gagcaacgcg gtattggcgg aacaattcct
     2341 gcaatacttc gacggcttct ccatcggctc gaacgacatg acccagctga ccctcggcct
     2401 cgaccgagac agcggcttgg tgtccgaatc gtttgacgaa cgcaaccctg ccgtcaaagt
     2461 gatgctgcat cttgccatct ccgcctgccg caagcagaac aaatatgtcg gtatctgcgg
     2521 tcaaggtccg tccgaccatc cggactttgc caaatggctg gttggagaag gcatcgaaag
     2581 cgtgtctctg aacccggata ccgtcatcga aacttggctg tatttggcga atgaattgaa
     2641 caaataatca attcccatac ccccaagcct gaaaagcgcg ggggtatttt ttccaagcag
     2701 ctccgttcag acggcatttc ctgtcgatgc cccgtccgcg ataatatttg acacccacgc
     2761 gccgactgcc tacaattccc ccctctccga gcaaccggca acagtcagct tcttctttca
     2821 gacggcatcc acccgtcttt tcctttctat tctatatatc attgattatg cacgtctccg
     2881 aattacaaac cctgcacatt tccaaactct tagaattggc ggaagaacac ggcatcgaaa
     2941 acgccaaccg attccgcaaa caagacctcg tatttgccat cgtccgccag atgatgaaaa
     3001 aaggcgaggg ttttacctgc tccggcacgc tcgaaatcct gcccgacggc ttcggcttcc
     3061 tccgcagcgc ggacacgtcc tatcttgccg gccccgacga catctatgtc tcgcccactc
     3121 aaatccgccg cttcaacctg catacgggcg acaccatcga aggcagcgtg cgcgtcccca
     3181 aagacaacga acgctatttt gccttggtca gactggattc catcaatggc gaccacccgg
     3241 aagtatgccg ccataaaatc ctgtttgaaa acctgacccc gctgtttccg accgaacagt
     3301 tgaagctgga acgcgactta aagtccgaag aaaacctgac cggacgcgcc atcgacctga
     3361 tttcgcccat cggcaaaggt cagcgcgccc tcttggttgc cccgcccaaa atcggtaaaa
     3421 ccgtgatgct gcaaaacatt gcccacgccg ttaccgcaaa ctatcctgaa gtcgaactca
     3481 tcgtcctctt gattgacgaa cgccccgaag aagtaaccga aatgagccgt tccgtccgag
     3541 gtgaagtagt ctcctccacc tttgacgaac cggcgcaacg ccacgtccaa gttgccgaaa
     3601 tggtgcttga aaaagccaag cgtatggtgg aacacaaaaa agacgtggtc atcctgctgg
     3661 attcgattac ccgccttgcc cgcgcctaca ataccgtcgt ccccgcctcg ggcaaaatcc
     3721 tgaccggcgg tgtcgatgcc aacgcgctgc accgccccaa acgtttcttc ggtgcggcgc
     3781 gcaacgtgga agaaggcggt tcgctgacca ttatcgccac cgcattggtt gaaaccggca
     3841 gccgtatgga cgatgtgatt tacgaagaat tcaaaggtac cggcaatatg gaattgcacc
     3901 tcgaccgccg tatggcggaa aaacgcctct tccccgccat caacatcaac aaatccggca
     3961 cgcgccgcga agagctgctt gtgccgaacg accagttgca acgtatgtgg ttactgcgca
     4021 agttcctgca cccgatggac gaaatcgaag ctaccgagtt cttaaacgga aaaatcaaag
     4081 catctaaaaa taatgatgat ttctttgaac tgatgcgcgg aaaataaacg cgccgcccgc
     4141 attaatgcga aatgccgtct gaagcctga
//
LOCUS       DAXFZR010000121         3960 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000121 DAXFZR010000000
VERSION     DAXFZR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.122"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..202
                     /locus_tag="ACFJAS_002037"
     CDS             <1..202
                     /locus_tag="ACFJAS_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983194.1"
                     /translation="NIWQGVKKGVHSATQNYRRSGAMPSERIFRSVLRLVGVSSDTVK
                     RKFAGAGLSYAGKAAGESLYCL"
     gene            318..1112
                     /locus_tag="ACFJAS_002038"
     CDS             318..1112
                     /locus_tag="ACFJAS_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC3983195.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            1337..3940
                     /gene="pepN"
                     /locus_tag="ACFJAS_002039"
     CDS             1337..3940
                     /gene="pepN"
                     /locus_tag="ACFJAS_002039"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC3983196.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGDPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTASDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQLALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLMT
                     EAEQAFPLEGVTEAVVPSLLRGFSAPVYLNYPYSDDDLLLLLAHDSDAFTCWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAKQENQSYEYSPETADWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFNLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT          945 a         1096 c         1096 g          823 t
ORIGIN      
        1 aaatatttgg caaggtgtga aaaaaggcgt acattccgct acacagaatt acagacgcag
       61 cggagcaatg ccgtctgaaa ggattttccg gtcagtcttg cggttggtcg gagtttcatc
      121 ggatacggtg aaacgaaagt ttgccggcgc agggttgagc tacgcgggta aagccgcagg
      181 cgaaagcctg tattgtttgt gaagcgtaaa tctctgattt gaggtattgg ggcaatcctg
      241 tgggggttgc ctcttttttt atccgccttt taatgacaca ataggcgcaa gtctgttttt
      301 gaatgcaagg tactgccatg aatacgattt tggctttcga tattgaaacc gtacccgatg
      361 tgcagggtat ccgtacgttg tatgacttgc cgtcttccct gccggatgac gaagtggtgc
      421 tgttcgcgca gcagaaacgc cgcgcacaga ccggcggcga ttttatgcag caccacctcc
      481 atcaggttgt ggcggtttcc tgctgtatgc gctgggggca ggacaaggtt catgtcggca
      541 ccatcggcga gatggacgat ggcgaagaag tcgtgattgc caagtttttc gaattggttg
      601 aaaaacatac gccgcaactg gtcagctgga acggcggcgg tttcgacctg cccgtactgc
      661 attaccgctc cctgatatac ggcatcaacg ccgcgcgcta ttgggatatg ggcgacggcg
      721 atttcggcga cagccgcgat ttcaagtgga acaactacat cagccgttat caccaacgcc
      781 actgcgattt gatggatttg ctcgcgcttt accagccgag ggcgaatgtg ccgctggacg
      841 atatggcgaa actgtgcggc tttccgggca agctgggtat ggacggcagc aaggtgtggg
      901 aggcgttcca cgcgggcagg ctgaaggaaa tccgcaatta ttgcgaaacc gatgccgtga
      961 atacgtattt gatgtatctg cgtttctgtc tggtcagcgg cagattcgac gcggacgaat
     1021 acgaaatgga aatcaagcgg atcagaaact atctctccgc ccaaacagaa gacaaaccgc
     1081 attgggcaga atttgtccaa gcgtggaaat agaaacctgc tgtccgaagg cggtacgctt
     1141 gtgccgccgt ttcggtttca gacggcgtat ggtggattaa attcaaaccg gtacagcctt
     1201 gcctctcctt gccttactat ctgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
     1261 ttaatccgct ttaaaagctg tatccgtgcc gtctgaagcc ttttccagtc aaaccaaaca
     1321 atattgaaag aaaaaaatga gcaaaaccgt ccgttatctg aaagattacc aaacgcctgc
     1381 ctaccgcatt cttgaaaccg aactgcattt cgatattgcc gaaccgcaaa ccgtcgtgaa
     1441 gtcgcgtttg acggtcgagc cgcagagggc gggcgatccg ctggtgttgg acggttcggc
     1501 aaaacttttg tccgtcaaaa tcaacggcgc ggcggcggat tatgtgttgg aaggcgagac
     1561 gctgacgatt gcagacgtac cgtccgaacg cttcaccgtc gaagtggaaa ccgaaatcct
     1621 gccggcggaa aacaaatcgc tgatggggct gtatgcttcc ggcggcaatc tgtttaccca
     1681 gtgcgagccg gagggcttcc gcaaaatcac gttctacatc gaccgtccgg atgtgatgtc
     1741 caagttcacg accaccatcg tcgcggacaa aaaacgctat cccgttttgc tttccaacgg
     1801 caacaaaatc gacggcggcg agttttcaga cggccgccat tgggtgaaat gggaagaccc
     1861 gtttgccaaa ccgagttatc tgtttgcttt ggtcgcgggc gatttggcgg taacggaaga
     1921 ccgtttcacc accatgagcg gcagaaacgt caaaatcgag ttttacacca ccgaagcgga
     1981 caaacccaag gtcggctttg ccgtggaatc gttgaaaaac gcgatgaagt gggacgaaac
     2041 gcgcttcggg ttggaatatg acttggatat tttcatggtc gtcgccgtag gcgatttcaa
     2101 tatgggcgcg atggaaaaca agggtttgaa catttttaac accaagttcg tcctcgccga
     2161 cagccgcacc gccagcgata ccgatttcga aggcattgaa tccgtggtcg gacacgaata
     2221 tttccacaac tggacgggca accgcgtaac ctgccgcgac tggttccagc tttcgctgaa
     2281 ggaagggctg accgtgttcc gcgaccaaga gttttccggc gaccgcgccg gccgcgccgt
     2341 gcgccgcatc gagaacatcc gcctgctgcg ccagaaccag ttccccgaag acgcaggccc
     2401 gaccgcccat ccggtgcgcc ccgtcagcta tgaggagatg aacaatttct acaccatgac
     2461 cgtttatgaa aaaggcgcgg aagtggtgcg gatgtatcat accctgctcg gcgaagaggg
     2521 cttccaaaaa ggcatgaagc tatatttcca acgccacgac ggacaggcag tgacctgcga
     2581 cgatttccgc gcggcgatgg cggatgcgaa cggcatcaat ctcgaccagt tggccttgtg
     2641 gtacagccag gcgggcacgc ccgttttgga agccgaaggc cgtctgaaaa acaatgtttt
     2701 cgagttaacc attaaacaaa ccgtgccgcc cacgcccgat atggcggaca aacagccgat
     2761 gatgattccc gtcaaagtcg ggcttctgaa ccgcaacggc gaagcggtgg cattcgatta
     2821 tcagggcaaa cgcgcaaccg aagccgtgtt gctgatgacc gaagccgaac aggccttccc
     2881 gctcgaaggt gtaaccgaag ccgtcgttcc ctcgctgctg cgcgggttca gcgcgccggt
     2941 gtatctgaac tatccgtaca gcgacgacga cctgctgctc ctgctcgccc acgacagcga
     3001 cgctttcacg tgctgggaag ccgcccaaac gctctaccgt cgcgccgtcg ctgccaacct
     3061 tgccgcgctt tcagacggca tcgggttgcc gaaacacgaa aaactgcttg ccgccgtcga
     3121 aaaagtcatt tcagacgacc tcttggacaa cgccttcaaa gccctgcttt tgggcgtgcc
     3181 gtccgaagcc gaactgtggg acggcacgga aaacatcgac ccgctgcgct accatcaggc
     3241 gcgcgaagcc ttgttggata cgcttgccgt ccgcttcctg ccgaaatggc acgaattgga
     3301 ccgtcaggcg gcgaagcagg aaaaccaaag ttacgaatac agccccgaaa ccgccgactg
     3361 gcgcacgctg cgcaacgtct gccgcgcctt cgtcctgcgc gccgaccccg cgcacatcga
     3421 aactgttgcc gaaaaatacg gcgaaatggc gcaaaacatg acccacgaat ggggcatcct
     3481 gtccgccgtc aacggcaacg aaagcgatac gcgcaactgc ctgctggcgc agtttgccga
     3541 caagttttca gacgacgcgc tggtgatgga caaatatttc gcccttatcg gctcaagccg
     3601 ccgcagcgac accctgcaac aggttcaaac cgccttgcag catccgaaat tcaatctcga
     3661 aaaccccaac aaagcccgtt cgctcatcgg cagcttcagc cgcaacgtcc cgcattttca
     3721 cgcacaagac ggcagcggct accgcttcat cgccgacaaa gtcatcgaaa tcgaccgctt
     3781 caacccgcag gtcgccgccc gcctggtgca ggcgttcaac ctctgcaaca agctcgagcc
     3841 gcaccgcaaa aacttggtga aacaagaatt gcagtgcatt cgggcgcagg aaggattgtc
     3901 gaaagacgtg ggcgaaatcg tcggcaagat tttgggttga ggcagtcaaa cagaaaaaac
//
LOCUS       DAXFZR010000122         3892 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000122 DAXFZR010000000
VERSION     DAXFZR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.123"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..761
                     /locus_tag="ACFJAS_002040"
     CDS             <1..761
                     /locus_tag="ACFJAS_002040"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC3983197.1"
                     /translation="KMKKRELNEIETAECAELKRIFNSKKEELKLTQYKLAEAVGVTQ
                     SAVNHYLNGTNALNASIASQFAKILQIPVSDFSLRLAEEISSMSIGIDGDKLLALQAD
                     NLDTNTITLNLYDVSASCGHGVVNPDYPQLLRSIEIPNDALFELLGTNNLTNVQLMPP
                     DGDSMEPTIPQKSITLIKTDVSKFQTGGIYLFTFDGYTYIKRLSRGKGGAIHATSDNR
                     HYAKSDFLINPEEADKFHIHGKFWKVLPLDFLDL"
     gene            1034..1852
                     /locus_tag="ACFJAS_002041"
     CDS             1034..1852
                     /locus_tag="ACFJAS_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFC3983198.1"
                     /translation="MNRYAMRFAVIRFMPYVQTREFANIGIIITHPQSGCFDFKIEHR
                     YSRLSRFFRRFDPPAYKAATRAFAEELQRIGKLAAYSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVARSFAKSQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGQKDPADIYYKSDKWIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVLGDLEEQGIRLADNRSEGKIIRNFACG"
     gene            2022..2204
                     /locus_tag="ACFJAS_002042"
     CDS             2022..2204
                     /locus_tag="ACFJAS_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFC3983199.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            2306..2707
                     /locus_tag="ACFJAS_002043"
     CDS             2306..2707
                     /locus_tag="ACFJAS_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC3983200.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            2839..3474
                     /locus_tag="ACFJAS_002044"
     CDS             2839..3474
                     /locus_tag="ACFJAS_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFC3983201.1"
                     /translation="MPDLDCLLYRKPHIIKSMKPHNDTIFQEPKAAQAAAFLLYKANG
                     RLEVLKLMKLMYLAERESFLRFGEGLTGDALVSMPHGPVLSMTLDFINGGHESVPNGW
                     ATWVSDRENRMLALRDPSMIRTPGQDLSALSEADLEVLESVWENYGHYSAWDLRNMTH
                     NGLCPEWEDPHGSSRPIPIKKLLSVLGYDDEQAVAIVERLEEQAYINRAFG"
BASE COUNT         1099 a         1034 c          913 g          846 t
ORIGIN      
        1 gcaaaatgaa aaaaagagaa ttaaatgaaa ttgagacggc tgaatgtgcc gaactaaaaa
       61 gaatttttaa ctcaaaaaaa gaagaactta agctaaccca atacaagctg gcagaagctg
      121 taggagttac tcaaagcgcg gtgaaccatt atttaaatgg caccaacgcc ttaaacgcct
      181 ccattgcaag ccagtttgcc aaaatcttgc aaattcctgt ttcggatttc agcctccgac
      241 ttgccgaaga aataagcagt atgtccatcg gcattgatgg agataaattg ctggcactgc
      301 aagcagacaa tctcgacacc aacaccatca ccctcaacct atacgacgta tcagccagtt
      361 gcggtcatgg ggtagtcaac cccgactatc cgcaactgct acgctcgatt gagattccca
      421 atgacgcgct attcgagctt ttggggacaa acaatctgac aaacgtgcag cttatgccgc
      481 cggacggcga cagcatggag ccgaccattc cacagaaatc gataacttta atcaaaacag
      541 acgttagcaa gtttcagacc ggcggcatct acctgtttac ctttgacggt tatacctaca
      601 tcaaacgcct gtccagaggc aaaggcggcg cgatacacgc taccagcgac aacaggcatt
      661 acgccaagtc ggattttctg ataaaccctg aagaagccga caaattccac attcacggca
      721 aattttggaa agttttgccg cttgattttt tagacctttg accgcccctt tggacagcct
      781 gaccgtcatc ggcccggtct tccggcaggg cgtgccggat tgacaaattc ctcccgggaa
      841 atctcaaaaa gtcggattta cagatttaca caaagtgtaa atttcatagg gtaaagaaaa
      901 gaaaagcgtg tgataatctg ccgccggagc gggcacgggc aaacgaagag cgcgacctgc
      961 ccgccgccta taaccgcagc cgcaccgacg aggccgtccg ccgcatcggt aacggaacac
     1021 tttggagcat ctcatgaacc gatatgccat gcgttttgcc gtcatacggt ttatgcccta
     1081 tgtccaaacg cgcgaatttg ccaacatcgg catcatcata acccacccgc aaagcggctg
     1141 cttcgacttt aaaatcgaac atcgctacag cagattgagc cgttttttcc gccgcttcga
     1201 tccgcccgcc tataaagcgg cgacccgtgc ctttgcagaa gaattacagc ggattgggaa
     1261 attggcggca tactccgcgc ccgatcagat acgcgccatg ctcgaccatt tgacccgccc
     1321 gcgcgaagcc ctgattatgg ccgcccaacc gggcgtaacc ctcgcccccg acagggggca
     1381 ggaactgaac cgcctgtttg attattttgt cgcccgcagc tttgctaaaa gccaacccga
     1441 agcagaactt acccgacaaa tacaggcaat gttaaagccg cttcagacgg catacccctt
     1501 caaagaaagc acaatcggcg acccgtcagg ctttcacgcc tccatcccct tggtgcaaaa
     1561 ggcggaaaac ggcgaaatac ggaaaatcat caagcctatc tatttcggac agaaagaccc
     1621 tgccgacatc tattacaagt ctgataagtg gattgccggc ataaaacggt tgcggcgcgg
     1681 cggatacatc gaccgctctg aaatattgtt tgcctacgaa ccgccggagc gtcccgacaa
     1741 ggcacaagaa aaggcattgc ttgacgtatt gggcgacctg gaggaacagg gcatacggct
     1801 tgccgataac cgaagcgaag gcaaaatcat cagaaacttt gcctgcggct gacagccctc
     1861 aagaccgccc gcgttatgcg ggctttttac acccttccct gcaactactg ccgaaagata
     1921 tttttacaca caaatacaca taaaaattga caaaataaaa attagtgtgt attattacgc
     1981 acattggcag gcgtgccatg cattaagatt aggagtgata attgaatagc ctagacgtta
     2041 ttgccctact caaacaagac ggttggtata aagttgcaca atccgggagc cattcgcaat
     2101 ataagcaccc aacaaaaaaa ggccgtgtaa ccgtgccgca cccgaaaaaa gacttgccga
     2161 caggtactgt aaaaaatatc tataagcaag ccggtttgaa gtaacggcag gcagcggggt
     2221 ttcccgccgc cccccttcta atcaaacaat cgacgggtta tctcacgcgc ccataaacct
     2281 gaacaacata attaaaggaa atcaaatgtt tatccctgcc gccctgcaca aagacgaaca
     2341 ttcggcatat ggcgtaacca ttcccgacct tcccggctgt ttctcttgcg gcgacaccgt
     2401 tgaagaagcc gtcgccaacg cccgttctgc cgcctatatg catattgacg gtatgattga
     2461 ggacggaggg ttcaaaaacc ttgccgtaag cagcattgcc gatttgagcc aagaacctga
     2521 ttatcatggg gcaacatggg taatgattga aatcgaccct gcaaaaatca gtcggcagca
     2581 aatccgattc aacgtcagct ggccgcaata ccttcttgat agagtggatg aatatacatc
     2641 cgcaaatcat gaaacccgta gcggtttttt agccaaagcc gccctgctca ccatgaatca
     2701 ggcatagcca gcccgttcaa cccgcccgca cacgcgggct ttttccacgc ccgccgaacc
     2761 tgaagacaac acaaacggtt cgtgccggat tgaggaatat gctgaaatat atcgaaaaga
     2821 tgtaaaatgt acggaaatgt gccggattta gattgcctgc tttacaggaa accgcatata
     2881 atcaaaagta tgaaaccgca caacgatacc attttccaag agccaaaggc tgcccaagcg
     2941 gccgccttcc tgctgtacaa ggcaaacggc aggcttgaag tactgaagct gatgaagctg
     3001 atgtacttgg ctgaacgtga gtctttttta cgcttcggag aaggcctgac aggtgacgcg
     3061 ttggtttcaa tgccgcatgg cccggtactc tccatgacct tggatttcat taacggcgga
     3121 catgagtccg ttccgaacgg atgggcaacg tgggttagcg accgcgaaaa caggatgctt
     3181 gccttacgcg atccaagtat gattcggacg cccgggcaag acttatcggc attatccgaa
     3241 gctgatttgg aagtactgga atcggtttgg gagaattacg gccattattc cgcatgggat
     3301 ctgcgcaaca tgacccacaa cgggttatgc ccggaatggg aagaccctca cggctcaagc
     3361 cgcccgattc ccatcaaaaa actgttgtct gtgcttggtt atgatgacga acaagccgta
     3421 gcgattgtag aaaggctgga agaacaggct tacatcaatc gcgctttcgg gtagggggcg
     3481 gtatgcctca atcgtggcaa tgcaggcaag gcgcatgcct gctcatccca tccggtccaa
     3541 atagctgcca acacttgttt accatcctag tcaatccgtg tatcctgccc aaccgtggaa
     3601 ataagccgca ggttctttct gtcggcattt cttccgtccg ggcaaatatc ccatatgata
     3661 atgcctgcat catcaggcag ggagaacatc cttttgtccg acatgacagt tatgtccgct
     3721 accgtgatgc aaggattgat gccgtcgaac acatagaaaa ccgtgtgcat gaaggaatat
     3781 tctccgtaaa accaccatgt ggcgaacaac tgttgaagcg tatcattgcc ggggcttcaa
     3841 catcacgata tgccggcagg gaagttaagc ttctgattga aaaatttgct at
//
LOCUS       DAXFZR010000123         3704 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000123 DAXFZR010000000
VERSION     DAXFZR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.124"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..1016)
                     /locus_tag="ACFJAS_002045"
     CDS             complement(<1..1016)
                     /locus_tag="ACFJAS_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFC3983202.1"
                     /translation="MSDLVRYDPLEHGRLAGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWDKGAGVGGG
                     LSNVWGELKKDWREKGLDGALEDVGEMGGAVLEQAPNALVPIATATAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIGRGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAADKAAVAAAKGTPEFAALAAK
                     GSAKGGLGGAARHAAAYATEAAGEFAGEYLGTGLANGEWDEKGAALEAFSSLGHSAVG
                     FAGT"
     gene            complement(1013..2209)
                     /locus_tag="ACFJAS_002046"
     CDS             complement(1013..2209)
                     /locus_tag="ACFJAS_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983203.1"
                     /translation="MGLFEPSAGDFWEMKEKEEKEKARKGAEERERAAAQAHRADVVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRGGKPVRDFDAQRPLVSSKPDALSPEE
                     RELYKRATTPHAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQKGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAQLGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(2447..>3704)
                     /locus_tag="ACFJAS_002047"
     CDS             complement(2447..>3704)
                     /locus_tag="ACFJAS_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983204.1"
                     /translation="FFGFREDNTGIPYGYVRNMKYAQDNLNSTNSKLRWGLSAIRTVR
                     TKGIVDMSDEQFRRNIARVDADIVLNKIEAAQPGARFDVSRDFELSAQHWQMLQDSRA
                     TIRQISGITPSFMGNRGNATSGRQESIQVEQSNQSLGLVMDNFRQSRSLVGELLLAMI
                     IEDLGSDEQTVVIEGDAITQGRTVVINRPETDPVTGKAYLSNDLQNIRLKVALEDVPS
                     TNSYRSQQLGAMSEAVKSLPPEYQAAVLPFMVSLMDIPFKDKVIEKIKKVRVQETPEQ
                     IEARIAQAVQDALAKSGNDIKRRELALKEQRTASEIKEIEARAVQIGVQAAYAAMQAG
                     GQIAAMPQIAPVADAVMQGAGYVRPARGDDPGFPVPAMPPETQIPPEGIPEAYGADTG
                     PMTAVPPKSANHAQTGMETPTVSDNL"
BASE COUNT          694 a         1375 c          864 g          771 t
ORIGIN      
        1 ttcgttccgg caaaccccac cgcagaatgc cccaaagaag agaaagcctc caaagccgcc
       61 cccttctcgt cccattcccc gtttgccagc cccgtaccca aatactcgcc cgcaaactca
      121 ccggccgctt ccgtcgcata agccgccgcg tgccgtgccg caccgcccaa accgcccttg
      181 gcagacccct tcgccgccag cgccgcaaat tcaggcgttc ccttagccgc cgcaaccgcc
      241 gccttatccg ccgccgcaac acccattttc tccaacgcgg ctttcccggc ggcctttttg
      301 cccatattca aaatactgcc gccaagtttc atcgccgcca tatccgccgc gccgaccacc
      361 gcgcccttga ccgccgcatt tttcaacgca cccggcgcac cgcggccgat aaacgccatc
      421 accgcatcct tgtccgcagg gtcgacgccc gccgcctctg ccgccctgtc cagctgcccg
      481 ccgtattcca tcagcgtatt gcccaaggtc gcgcccgcat aggcgccgac agccgcgtta
      541 ccgcccgcca aagcgcccaa tatgccgccg gcggttgccg tagcaatagg gacaagcgca
      601 ttgggcgcct gctccagcac cgcgcccccc atctcgccca catcttccaa ggcgccgtcc
      661 aaacccttct cgcgccagtc tttcttaagc tccccccaca cattcgacag gccgccgccg
      721 acacccgcgc ccttatccca agcctcgccc agctccctgc gcgcttggga tttgcgcccc
      781 tcctgaatct tccgataatc catatcctcg gccttgagcc ggcccagttc ctctttgtcg
      841 cccgtaaggg cattccaccc catacgcgcc gaacgcatag aatttttaaa cccgcgcgtc
      901 aacgccgtat cgtcggcggc cgcccgcgca tccttttgcg taaagccgcg gtactccttc
      961 aaacccccgg caagccgccc gtgttccaac ggatcgtatc tgaccaaatc actcattgct
     1021 caaccctctt ctcacccttc tcattctcat aaatgatttt accgccggcc gttttctgac
     1081 cgttcggctt aaagcccaac tgcgcataac gcttctcgcg ctctcccgca aactccacat
     1141 ccaaaggctt gcccgtccgc aaatcgacaa tcccctcgcg cttcgccgtc aagcccgtat
     1201 ccgggtcggc aacctcataa ctgatagtct tgaacacatt cgggtcaaag cccttctccc
     1261 cttgccgctc aaacttcccg ttaagcgcat caatctgccg cgcaatctcc ccgcgctgcc
     1321 cgtccgactt cgcaaaacca taagcccgcg tcaaatcggc gatgcgcttc ctatcctcaa
     1381 gctccagcgc atccgcctca cgcttcatct caaacccctg ccgcttcatc cccagctcgg
     1441 cctgctgcac atcaaacgcc ctgtccgcat tcctctgcgc catctcgcgg ttcaacgcac
     1501 cctcgttcgc cttaaccgcc gcatcaaacc gccccttctg caacaccgcc tcacggttcg
     1561 ccgcattctc cgccgcagcc gccgccagcc tttccctgcc caattccctg accgccgcat
     1621 ttttattatg ttccgccaca atcccgcgcg ccgcattaag ctgcgccgcc gtcaactgac
     1681 cgttcaaggc ccccgcatga ggcgtagtcg ccctcttata aagctcccgc tcctcaggcg
     1741 acagcgcatc cggcttggaa gacaccaaag gacgctgcgc gtcaaaatca cgcaccggct
     1801 tgccgccccg atacacattc ccgtcaaatc ccaaaggcgg cggcgtccgt ccgcccgcat
     1861 ccgtcgggcg cgtatcgcgc cgcgcgaaat ccgccccggc ccgcgcatcg ggggaaagcg
     1921 ccttatcgcc gcccgaaaac cccaaaccgg catccgcacc ccgttcgaaa gcacccgccc
     1981 ggcgcaaccg ccgcgcccca tcctcaatat tgttacggct caaatccatc acattccgat
     2041 aacgcgccgg cccggcctca taattcgcaa cggtacgccg caccacatcg gcacggtgcg
     2101 cctgtgccgc cgcccgctcc cgctcctccg cccccttccg ggccttctct ttttcctcct
     2161 tttccttcat ctcccaaaaa tccccggcag acggctcaaa caaacccatc gcaaaccctc
     2221 cgaacaaata aagtccaaaa taagcaaaaa cccaagccca atcaaacgcc taaagttaag
     2281 aacaaacaaa aatcgcaacc ccggaccaat taacaaacaa caagttacac aaaaaaaaca
     2341 caccgacggg ccatttccgg gggcaaataa aaaccgcctt cgtgcaaggc ggttgcaaaa
     2401 aagcctttca tttaacgaaa ggctttttgc cgcccgcgta ccgcgttcag aggttgtccg
     2461 acaccgtcgg cgtttccatg cctgtttggg cgtgattcgc actctttggc ggcacggccg
     2521 tcatcgggcc ggtatccgcg ccgtaggctt cagggatgcc ttcgggcggt atttgcgttt
     2581 caggcggcat ggcggggacg gggaagccgg gatcgtcgcc ccgcgccggc ctgacatatc
     2641 ccgcgccctg catgaccgcg tcggcaacgg gggcgatttg cggcatggcg gctatctgcc
     2701 cgcccgcctg catggccgca taagccgcct gcacgccgat ttgtaccgcc cgcgcttcga
     2761 tttccttgat ttcgctcgcg gtacgttgtt ccttgagcgc caattcccgc cgtttgatgt
     2821 cgttgccgga ttttgccaat gcgtcctgca ccgcctgcgc gatacgcgcc tcgatttgtt
     2881 cgggcgtttc ctgcactcgg acttttttga ttttttcaat cactttgtct ttaaacggga
     2941 tgtccatcag ggacaccata aacggcagca ccgccgcctg atattcgggc ggcagggatt
     3001 tgaccgcctc gctcatcgca cccagctgct ggctgcggta ggagttggtg ctgggcacgt
     3061 cttccaaagc cactttcagc cgtatgtttt gcaggtcgtt ggacaaataa gccttgccgg
     3121 ttacggggtc ggtttcaggc ctgttgatga cgaccgtccg cccttgcgtg attgcgtccc
     3181 cttctatgac gacggtttgc tcgtccgagc ccaaatcctc gataatcatc gcaagcagca
     3241 actcgccgac caatgagcgg ctctggcgga agttgtccat aaccagcccc agcgactggt
     3301 tggactgctc gacttggatg ctttcctgcc tgccgctggt ggcgttgccc cggttgccca
     3361 taaatgacgg ggtaatcccg ctgatttgcc gtatggtcgc gcggctgtct tgaagcatct
     3421 gccaatgctg tgccgacaat tcgaaatcgc ggctgacgtc gaaacgcgcg cccggctggg
     3481 cggcctctat tttgttcagc acgatgtccg cgtccacccg tgcgatattg cggcggaact
     3541 gttcgtccga catatcgact atgcctttgg tgcgtaccgt gcgtattgcc gacaaacccc
     3601 atcgtaattt gctgttggtg ctgttgaggt tgtcctgcgc gtatttcata ttgcggacat
     3661 atccgtaggg gatgccggtg ttgtcctcgc ggaatccgaa aaac
//
LOCUS       DAXFZR010000124         3699 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000124 DAXFZR010000000
VERSION     DAXFZR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.125"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            663..>3699
                     /gene="pilC"
                     /locus_tag="ACFJAS_002048"
     CDS             663..>3699
                     /gene="pilC"
                     /locus_tag="ACFJAS_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC3983205.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAMAQTRQYAIVMNER
                     KQPEVKWESQYNQSTLKGKGRERKFIYSKGRRGGGSVSFNNSDELVSQQSGTAVFGTA
                     TYLPPYGKVSGFDTAELNKRGNAVNWIHTTRPGLAGYVYTGVHCGSYQCPKLTYKTRF
                     SFENTGLAKNANRLDKHPDPSRDNSPIYKLKDHPWLGVSFNLGAEGTAKDGRSSNKLV
                     SSFDENNSNSNQNLVYTTEGHPISLGDWQRESTAMAYYLNAKLHLLNKKGIKDIAQNK
                     TVDLGTLKPGIDVKSANTGLGGILSFHAQWDIKDTGQIPVKLGLPEVKAGRCINKPNP
                     NNNTKAPSPALTAPALWFGPGQDGKVQMYSASVSTYPDSSSSRIFLQELKTRTDPARP
                     GRHSLAALNAQDIKSREPNFNSRQTVIRLPGGVYRIGPTRDGIVGLNGNDGKNDTFGI
                     YKDRLVTPEVDEWAKVLLPWTARYYGNDDIFKTFNQPNNKKQSDKKQYSQKYRIRTKE
                     DDNDKPRDLGDIVNSPIVAVDGYLATSANDGMVHIFKKTGTDERSYNLKLSYIPGTME
                     RKDIEGNDSDLAKELRTFAEKGYVGDRYGVDGGFVLRRITDDQDKQKHFFMFGAMGLG
                     GRGAYALDLSKIDSSNLTGVSMFDVQNDKNNNKNDNNRVKLGYTVGTPQIGKTQNGKY
                     AAFLASGYAAKDINSNDNKTALYVYDLNNTLGTPIAKIEVKDGKGGLSSPTLVDKDLD
                     GTVDIAYAGDRGGNMYRFDLSDNDPNKWSVRTIFEGGKPITSAPAVSRLADKRVVIFG
                     TGSDLSEQDVVGTDQQYIYGIFDDDKPTVNVKVTNGTGGGLLEQVLSEENKTLFLTNN
                     KASGGSADKGWVVKLREGERVTVKPTVVLRTAFVTIRSYTGNDKCGAQTAILGINTAD
                     GGALTPRSARPIVPDHNSVAQYSGHKKTSKGKSIPIGCMEKGGKTVCPNGYVYDKPVN
                     VRYLDETETDGFSTTADGDAGGSGTFKEGKKPARNNRCFS"
BASE COUNT         1014 a          985 c          983 g          717 t
ORIGIN      
        1 aaggcaactt agatttgcag ctgttgtttc aggtcgtctg aaaaataaaa agcaacctgc
       61 acaacctgtt ttccttgcag caaacctcaa ccccaacagc cgacccgtcc tctctccctg
      121 tgggagagag ctagagagag ggcaacaagc cacaaggctt gtattggggc ggttaagttg
      181 ttgggaaagg ttgccgcaac ttggagaatg ccctctcccc ggcccgccat tgccgcgcag
      241 gcggcaatgg cgggccgggc aaacggttgc ggattgccaa aaaccgctgt accatggata
      301 aggataagcg cgcaaggata gaatgatgcg gcaaccctat acattgcacc ccgtcagagg
      361 ggcgcgttac ctttgcgaac atcccccttt ggcagccggg cgaagggggc tttgcaaccg
      421 gcaatccggc gggcgcggga tcgggcggtt tgccgaatcc cgccgtttgc ggcgcgcctg
      481 ccgccgacgg tatcccgcga agcaagattt aagggataaa atatgttcca acacacaggg
      541 cggcacataa ggcgccgccc tgattcggaa gggcttgcac ccctcccgga caaagcctga
      601 tcctgccgcc ccgaaggacg gatgcccgaa gggcgggggg gtttgaccga aaaggaaata
      661 cgatgaataa aactttaaaa aggcaggttt tccgccatac cgcgctttat gccgccatct
      721 tgatgttttc ccataccggc gggggggggg cgatggcgca aacccgtcaa tacgctattg
      781 tcatgaacga gcgaaagcag cccgaggtaa agtgggagag tcaatataat caatcaacat
      841 taaagggcaa aggcagggaa cgcaaattta tctatagtaa aggcagacgg ggtggaggct
      901 ctgtctcatt caacaatagc gatgagcttg tttcccaaca aagcggtact gccgtttttg
      961 gcacagccac ctacctgccg ccctacggca aggtttccgg ctttgatacc gccgagctga
     1021 acaagcgcgg caatgccgtc aattggattc ataccacccg gcccgggctg gcaggctacg
     1081 tctacaccgg tgtccattgc ggaagctacc aatgccccaa acttacctat aaaacccgat
     1141 tttccttcga aaataccggt ttggcaaaaa atgcaaacag gctggataaa cacccggacc
     1201 caagccgcga caattcgccc atttacaaat tgaaggatca tccatggttg ggcgtgtctt
     1261 tcaatttggg cgccgagggt accgccaaag atggcagatc atccaacaaa ttggtatctt
     1321 cttttgatga aaacaatagt aatagtaatc aaaacctcgt ctatacgaca gaaggccacc
     1381 ctatttccct tggcgactgg cagcgcgaaa gtaccgccat ggcctattat ctgaacgcca
     1441 aactgcacct gctgaacaaa aaagggatta aagatatcgc ccaaaacaaa acagtggatt
     1501 tgggcacctt gaaaccgggc atcgatgtga agtcggcaaa tacggggctg gggggcatac
     1561 tatcttttca tgctcagtgg gacattaaag ataccgggca gatcccggtc aagctcggtc
     1621 tgccggaagt caaggccggg cgctgcatca acaaaccgaa ccccaataat aataccaaag
     1681 ccccttcgcc ggcactgacc gcccccgcgc tgtggttcgg acccgggcaa gatggcaagg
     1741 tgcagatgta ttccgcttcg gtttctacct accccgacag ttcgagcagc cgcatcttcc
     1801 tccaagagct gaaaactcga accgaccccg cccggcccgg ccggcattcc ctcgccgctt
     1861 tgaatgcgca ggatatcaaa tcccgcgagc cgaatttcaa ctcaaggcag accgtgatcc
     1921 gattgccggg cggcgtgtac cggatcggcc cgactcgcga cgggatcgtg ggtttgaatg
     1981 gcaatgacgg caaaaacgac actttcggca tctacaagga caggctcgtc acacctgagg
     2041 tcgacgagtg ggcaaaagtg ctgctgcctt ggacggcccg gtattacggt aatgacgata
     2101 tatttaaaac attcaaccaa ccaaacaaca aaaaacaaag cgacaaaaaa caatacagcc
     2161 aaaaataccg catccgcaca aaagaagatg acaatgacaa accccgcgat ttgggcgaca
     2221 tcgtcaacag cccgattgtc gcggtcgacg ggtatctggc aacttctgcc aacgacggga
     2281 tggtgcatat cttcaaaaaa accggcacgg atgaacgcag ctacaatctg aagctcagct
     2341 acatccccgg tacgatggag cgtaaggata ttgaaggcaa tgactccgac ctcgccaaag
     2401 agctgcgcac ctttgccgaa aaaggctatg tgggcgaccg ctacggcgtg gacggcggct
     2461 ttgtcttgcg ccgcattaca gatgaccaag acaagcaaaa acatttcttt atgtttggtg
     2521 cgatgggcct tggcggcaga ggcgcgtatg ccttggattt aagcaaaatc gacagcagca
     2581 acctgaccgg cgtttccatg tttgatgtcc aaaacgacaa aaataacaat aagaatgaca
     2641 ataatcgcgt gaaattaggc tacaccgtcg gtacgccgca aatcggcaaa acccaaaacg
     2701 gcaaatacgc cgccttcctc gcctccggtt atgcggctaa agacattaac agcaacgaca
     2761 ataaaaccgc gctgtatgta tatgatttga acaacacctt aggtacgccg attgcaaaaa
     2821 tcgaagtgaa ggacggcaaa ggcgggcttt cgtcccccac gctggtggat aaagatttgg
     2881 acggcacggt cgatatcgcc tatgccggcg accggggcgg gaatatgtac cgctttgatt
     2941 tgagcgacaa cgacccgaac aaatggtctg tacgcactat tttcgaaggc gggaagccga
     3001 ttacctccgc gcccgccgtt tcccgactgg cagacaaacg cgtggtcatc ttcggtacgg
     3061 gcagcgattt gagtgaacag gatgtagtcg gtacggatca acaatatatt tacggtatct
     3121 ttgacgacga taagccgacg gttaatgtaa aggtaacaaa cggcacggga ggcgggctgc
     3181 tcgagcaagt gcttagcgag gaaaataaaa ccttgttcct gacaaataat aaggcatccg
     3241 gcggatcggc cgataaaggc tgggtagtga aattgaggga aggagaacgc gttaccgtca
     3301 aaccgaccgt ggtattgcgt accgcctttg tcaccatccg cagctatacg ggtaatgaca
     3361 aatgcggcgc gcaaaccgcc attttgggca tcaataccgc cgacggcggc gcattgactc
     3421 cgagaagcgc gcgcccgatt gtgccggatc acaattcggt tgcgcaatat tccggccata
     3481 agaaaacctc caaaggcaaa tccatcccta taggttgtat ggaaaaaggc ggtaaaaccg
     3541 tctgcccgaa cggatatgtt tacgacaagc cggttaatgt gcgttatctg gatgaaacgg
     3601 aaacagacgg attttcaacg acggcagacg gtgatgcagg cggcagcgga acattcaaag
     3661 agggtaaaaa acccgcccgc aataaccggt gcttctccg
//
LOCUS       DAXFZR010000125         3541 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000125 DAXFZR010000000
VERSION     DAXFZR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.126"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            88..963
                     /gene="dapA"
                     /locus_tag="ACFJAS_002049"
     CDS             88..963
                     /gene="dapA"
                     /locus_tag="ACFJAS_002049"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC3983206.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAAGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAVPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFAVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRTALKASGQL"
     gene            972..2168
                     /gene="bamC"
                     /locus_tag="ACFJAS_002050"
     CDS             972..2168
                     /gene="bamC"
                     /locus_tag="ACFJAS_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC3983207.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTERPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERRAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2225..3157)
                     /gene="pip"
                     /locus_tag="ACFJAS_002051"
     CDS             complement(2225..3157)
                     /gene="pip"
                     /locus_tag="ACFJAS_002051"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC3983208.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWAGWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALFQAVEDILPRLL"
BASE COUNT          952 a         1149 c          791 g          649 t
ORIGIN      
        1 gtgcggccga tatgtttcac acaaacaaca aagtaagcta caatccgaag cataatcgac
       61 ctcactcaat gaataaaagg tttccgtatg ttacaaggta gcttggttgc cctgattacc
      121 ccgatgaatc aagacggcag catccattac gaccaactcc gccaattaat cgactggcac
      181 atcgaaaacg gcacggacgg catcgtcgct gccggcacga caggcgaatc cgccaccctc
      241 tccgtcgaag aacatttaag cgtaatcgaa gaaacggtca aacacgttgc caaacgcgtc
      301 cccgtcatcg ccggcacagg ggcgaacaac accgtcgaag ccatcgccct ttcccaagcc
      361 gccgaaaaag ccggcgcgga ctacaccctc tccgtcgtcc cctattacaa caaaccttcc
      421 caagaaggca tgtaccgcca tttcaaagcc gttgccgaag ccgccgcggt tccgatgata
      481 ctctacaacg tgcccggcag aaccgtcgtc agcatgaaca acgaaaccat cctgcgcctt
      541 gccgaaatcc ccaacatcgt cggcgttaaa gaagcgagcg gcaacatcgg cagcaacatc
      601 gaactcatca accgtgcgcc cgaaggcttc gccgtccttt ccggcgacga ccacaccgcc
      661 ctgccgttca tgctctgcgg cggacacggc gttattaccg tagccgccaa cgccgcgccc
      721 aagctctttg ccgatatgtg ccgtgccgcg ctgcaaggcg acattgccct tgcacgcgaa
      781 ctcaacgacc ggctgattcc gatttacgac accatgttct gcgaacccag tcccgccgca
      841 ccgaaatggg cggtatccgc cttgggcaga tgcgaaccgc acgtccgcct gccgctcgtt
      901 ccgctgacgg aaggcggaca ggcaaaagtc cgcaccgccc tgaaagcctc aggacaactt
      961 taaatatagc catgccgtct gaaccgttcg gacggcataa catgacaaac actttaatat
     1021 ccatcacaca ggatgacacg atgacccata tcaaacccgt cattgccgcg ctcgcactca
     1081 tcgggcttgc cgcctgctcc ggcagcaaaa ccgaacggcc caagctcgac taccaaagcc
     1141 ggtcgcaccg cctgatcaaa ctcgaagtcc cgcctgattt gaacaacccc gaccaaggca
     1201 acctctaccg cctgcctgcc ggttcgggag ccgtccgcgc cagcgatttg gaaaaacgcc
     1261 gcacacccgc cgtccaacag ccagccgatg ccgaagtatt gaaaagcgtc aaaggcgtcc
     1321 gcctcgagcg cgacggcagc caacgctggc ttgtcgttga cggcaaatcc cccgccgaaa
     1381 tctggccgct tctgaaagcc ttttggcagg aaaacggctt cgacatcgaa tccgaagaac
     1441 ccgccatcgg acaaatggaa accgagtggg cggaaaaccg tgccaaaatc ccccaagaca
     1501 gcttgcgccg cctattcgac acagtcggtt tgggcggcat ctactccacc ggcgagcgcg
     1561 acaaattcat cgtccgtatc gaacagggca aaaacggcgt ttccgacatc ttcttcgccc
     1621 acaaagcgat gaaagaagtg tatggcgaca aaaacaaaga cacgaccatg tggcagcctt
     1681 ccgcttccga ccccaacctt gaggccgctt tcctgacgcg ctttatgcaa tatttgggcg
     1741 ttgacggacg gcaggcggaa aacgcattgg caaaaaaacc gacccttccc gccgccaacg
     1801 aaatggcgcg tatcgaaggc aaaagcctga ttgtctttgg cgactacggc agaaactggc
     1861 ggcgcaccgg ccttgccctc gaccgcatcg gactgaccgt cgtcggtcaa aacaccgaac
     1921 gccgcgcctt cctggttcaa aaagccccga acgaaagcaa tgcagttacc gaacaaaaac
     1981 cggggctgtt caaacgccta ctgggcaaag gcaaagcgga gaaacctgcc gaacagccgg
     2041 aactgattgt ctatgccgag cctgtcgccg acggttcgcg catcgtcctg ctcaacaaag
     2101 acggcagcgc atatgccggc aaagacgcat ccgcactgtt aggcaaactc cattccgaac
     2161 tgcgttaaac cgttcaaaac aaaagcagct tccaaataga agctgctttt ttgtgcggaa
     2221 ctttttacaa caaacggggc aaaatatcct caaccgcctg aaacaacgca tccgccaaag
     2281 gcggatcgaa cgcacaatgc cccgcctgga ccaccctcaa ttccgcttca ggaaaggctt
     2341 tcgacagctc ccacgcactc tgcataggcg tacacaaatc ataccgcccc tgcacgataa
     2401 cggtcgggat atgccgtatt ttgccgatat tgttcaaaat cgccttatca ccctgcaacc
     2461 aaccgccgtt gacaaaataa tggttttcca aacgcgcgat tgccagtgag gcataagcat
     2521 cttcatccac tccctccggc tcgaaacgaa tcagatagct ttcccaaccc gcccaagcct
     2581 tcgcggcaga caggcacact tcttcatctt gatgaaacag caacccgtga tacgcctcaa
     2641 tcagccggtt ccgccgattt tcagcaatcg gcgcgacaaa tttttgccat tgttccggat
     2701 aaatccggct cacgccgccc gcctcgttca gccacgccgt ttcagacggc ctgcacaaaa
     2761 atatcccgcg caacaccaat ccctttaccc gttcgggatg ggtttgggca taagccagcg
     2821 acaaagtgct gccccacgaa ccgccgaaca ccagccattt cccgataccc agcatttcac
     2881 ggactttttc aatatccgcc accaaatccc aagtcgtatt gtcttccgca caagcatacg
     2941 ggtgcgaacg tccgcaaccg cgctggtcga tgatgacgat gcggaacaca tcaggattaa
     3001 aaaaaccccg acattcaggc gaagcccccg cgcccggccc gccgtgtaaa aaaatgaccg
     3061 gcacaccgtc gggattgccc gactcttccc aataaatttg atgaatttca gacacctgca
     3121 agtatccgct atgaaaaggc tgttttattt catacatcat cttctcccat attgcttctg
     3181 acaatccgaa catcccgaca cgcgacatcc cgcacgaaac acaagccgcc ccgaaaggca
     3241 aatgccgaaa aatcgcgata aaaaccatca atcggaaaat attgcataaa caggcgaaaa
     3301 tatatcattg aaaagaaaac ccatacaatg tctgacagaa acaagccgct ttgccattgt
     3361 ttcctaacag ttaaaccccg cccttcaagg cggcggggca gggcttgatt caaaatggcg
     3421 caagcccctg ccctcaaatc caacacgcag gattaaacca taatagcggc tttcttatta
     3481 tttcttattg aaacaccgcc cggaacccga tataatccgc ccttcaacat cagtgaaaat
     3541 c
//
LOCUS       DAXFZR010000126         3511 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000126 DAXFZR010000000
VERSION     DAXFZR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.127"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(1..239)
                     /locus_tag="ACFJAS_002052"
                     /pseudo
     CDS             complement(1..239)
                     /locus_tag="ACFJAS_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
     gene            426..710
                     /locus_tag="ACFJAS_002053"
     CDS             426..710
                     /locus_tag="ACFJAS_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC3983209.1"
                     /translation="MKLLAALIPLLMNVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            711..1898
                     /locus_tag="ACFJAS_002054"
     CDS             711..1898
                     /locus_tag="ACFJAS_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC3983210.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            2113..3069
                     /locus_tag="ACFJAS_002055"
     CDS             2113..3069
                     /locus_tag="ACFJAS_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC3983211.1"
                     /translation="MNIIGSDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKPAYYIVKTGQPYDAERHRLNQ"
     gene            3360..>3511
                     /locus_tag="ACFJAS_002056"
     CDS             3360..>3511
                     /locus_tag="ACFJAS_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC3983212.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFEDET"
BASE COUNT         1190 a          757 c          788 g          776 t
ORIGIN      
        1 ccgggatatc cggttgcctc tatgtatttg tcgggatcgg catcgacttt caacgataaa
       61 atctcttccg gctttttggc atccatttct tctttgtatt tcggattgcg gccaagggaa
      121 aaagaagccc cagctctgaa atcatcacct ttattgaccg cacaacctcc gccgttccaa
      181 tcaaatgtgc aacgatttaa aacaaaatta ttccaatcca attaagcaac tttagccaaa
      241 ggcaggcgaa gtaccgcact ttgcgggcga agccgcaaac agccgagaag cgcggggggg
      301 ggattggcga taagcgcgag ggggtgtccc cacagcgctg ccgcgccgcg aatgcggagc
      361 aatctttcag attaagaaac atttgtttaa tgaggcaaac gtgcctttta agaaagggag
      421 agcaaatgaa attgttggcc gcattgattc cgctcttgat gaacgtcgca ggccgtatat
      481 tgactgcatt aggcttgatg gctgttacct atgcgggtgt agatagattg gcagcccatt
      541 ttcaacaggc gataacccat agcataacgg gcgcgcctca agcaatgtta cagcttttct
      601 atataagcgg cggtggtact gttctaaaca ttcttttcgg cgcaatcgcc tttattctgt
      661 cctttaaaca aatgacaaaa ctcgcaacct caatcgggaa gaaaaaataa atggcagaaa
      721 tctgtttgat aaccggcacg cccggttcag ggaaaacatt aaaaatggtt tccatgatgg
      781 caaacgatga aatgtttaag ccagatgaaa acggcgtacg ccgtaaagta tttacgaaca
      841 tcaaaggttt gaagataccg cacacccaca tagaaacaga cgcaaagaag ctgccgaaat
      901 caaccgatga acagctttcg gcgcatgata tgtatgaatg gatcaagaag cctgaaaacg
      961 tcggcgcaat cgttattgtc gatgaggcgc aagacgtatg gcccgcacgc tccgcaggtt
     1021 cgaaaatccc cgaaaacgtc caatggctga acacacacag gcatcagggc atagatatat
     1081 ttgtattgac acaaggtcct aaactcttag atcagaactt gcgaacattg gttaaaagac
     1141 attaccacat tgcggccaac aaaatgggtt tgcgtaccct gcttgaatgg aaagtatgcg
     1201 cggatgaccc ggtaaaaatg gcatcaagtg cattttccag tatctacaca ctggataaaa
     1261 aagtttatga cttgtacgaa tccgcagaaa ttcacacggt aaacaaagtc aagcgttcaa
     1321 aatggtttta tgcattgccc gtcatcatat tattgattcc gctatttgtc ggtttgtctt
     1381 acaaaatgtt gggcagttac ggaaaaaaac aggaagaacc cgcagcacaa gaatcggcgg
     1441 caacagaaca gcaggcagta cttccggata aaacagaagg agaatcggtg aataacggaa
     1501 accttacggc agatatgttt gttccgacat tgcccgaaaa acccgaaagc aagccgattt
     1561 ataacggtgt aaggcaggta aggacctttg aatatatagc aggctgtata gaaggcggaa
     1621 gaaccggatg cacctgctat tcgcatcaag ggacggcatt gaaagaagtg acggagttga
     1681 tgtgcaagga ctatgtaaaa aacggcttgc cgtttaaccc atacaaagaa gaaagccaag
     1741 ggcaggaagt tcagcaaagc gcgcagcaac attcggacag ggcgcaagtt gccaccttgg
     1801 gcggaaaacc gcagcagaac ctaatgtacg acaattggga agaacgcggg aaaccgtttg
     1861 aaggaatcgg cgggggcgtg gtcggatcgg caaactgaag aaaacggcaa cagagaaaaa
     1921 agacccgtaa accgtttgaa tatagacggc ttacgggtct ttgtttcgcg caaagcaagg
     1981 gctaaggcag tcaggcagca aatcccgcaa tgtattaaaa cagacgcgta gaaatgccgg
     2041 ctgcctttat ccatcctcaa aattgaatat catcctagcc gtatcaaggc tgtataaata
     2101 aggaaaatac caatgaatat aatcgggtcg gacatctcaa aggacaccat agacgcaaca
     2161 ttgcataaaa caaacggaag tatccattac attaaattta agaataatga tgatggatta
     2221 aaacagttta gattgtggat aaagggaaac agaatcagaa aagtctatat cggcatggag
     2281 gcaacaggca tctattacga aaaggcagca gatatgcttt cttcctacta taccgtttac
     2341 gttatcaatc ccttaaaaat caaggactac ggaaaaagca ggtttaaccg caccaaaacc
     2401 gacaaagcag attcaaacct gatagcagat tacataaaaa ggcatcaaga tacattgata
     2461 ccgtatcaga tacccaaaaa caaagcactg caaaaactga ttaatcttaa aaaccaatta
     2521 cagcaacagc agaagcaaat taaaaaccgt cttcatagca ctgaagaaga cttcataagg
     2581 aacatacatc aagacttgat agataccata caggacaaga tggaacaggt aaaaatagcc
     2641 atatccgaac aaatcaaaaa acaaacggac aataaccatt accgcaatct tcaaaccatc
     2701 ccgagcatag gcaaagacac cgcatcagtt ctttatgcgc aactgacaga aaaacatttt
     2761 aaaaccgcaa accaatttgt atcctatgcc ggattaagtc ccgccatcat acaatcaggg
     2821 acaagcgtaa gaggtcgggg cagattgagc cgatacggaa acagacgatt aaaaagtacg
     2881 ctgtatatgc ccgccctttg tgcttaccgt tttaacgcat ttccgaaatt aataaataat
     2941 ctgaaaaaag cgggtaagcc aaagatggta attatcgttg ccatcatgcg caaactggcg
     3001 aagcccgcct attacattgt taaaaccggc cagccttacg atgcggaaag acaccgattg
     3061 aatcaataaa attcaacaaa attaaacggt tgcgcgaata tatttgtgta accgtgcatt
     3121 tgcatatcgt aaataaacgt aaataaaaat aacaatataa atcagcatgt tgcaactttg
     3181 ttttttattt tgtgttgacg ggcaacatat catctgcgcg gaaagttacc gtatcggcaa
     3241 acgcgactga aaaagacagc gtgttcggcg atgcggtgca cattgtgatt gcgacttaaa
     3301 acatagaacg agttgccaac ccaaagttta catctcgtct tttaccgaaa ggattaaaaa
     3361 tgacagaatt accgtggata gccgaagcga gaaggcacat cggtttgaaa gaaattcccg
     3421 gcgcgaaaca caatccgacg attgtgcaat ggctcaaaga gacgggcggc ttccccggcg
     3481 cggcaaagtc ttggtacttt gaagacgaaa c
//
LOCUS       DAXFZR010000127         3476 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000127 DAXFZR010000000
VERSION     DAXFZR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.128"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..657
                     /locus_tag="ACFJAS_002057"
     CDS             <1..657
                     /locus_tag="ACFJAS_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3983213.1"
                     /translation="AMGAWLEVLSEENNKSVLAAIARNGAIWDKPTRHEDIVAVFPFG
                     NPIHNNTMIMRRSVIDGGLRFDPAYIHAEDYKFWYEAGKLGRLAYYPEALVKYRFHQD
                     QTSSKYNLQQRRTAWKIKEEIRAGYWKAAGISVGADCLNYGLLKSTAYALYEKALSGQ
                     DIGCLRLFLYEYFLSLEKYSLTDLLDFLTDRVMRKLFAAPQYRKILKKMLRPWKYRSY
                     "
     gene            676..1518
                     /locus_tag="ACFJAS_002058"
     CDS             676..1518
                     /locus_tag="ACFJAS_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC3983214.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHCGTAGYIISR
                     EAMRFFLDRFAVLPAERIKAVDLMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQN
                     SMLGSDLEKDREQGRRHRRSLKVMFDLKRALGKFGREKKKRMESQRQAELEKVYGRRV
                     ILFK"
     gene            complement(1861..2586)
                     /locus_tag="ACFJAS_002059"
     CDS             complement(1861..2586)
                     /locus_tag="ACFJAS_002059"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC3983215.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTRLIMSINRACKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
     gene            2690..>3476
                     /locus_tag="ACFJAS_002060"
     CDS             2690..>3476
                     /locus_tag="ACFJAS_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC3983216.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRE"
BASE COUNT          760 a          769 c         1070 g          877 t
ORIGIN      
        1 gcgatgggcg cgtggttgga agttttgtcg gaagaaaaca ataaaagcgt gcttgccgcc
       61 attgcccgaa acggcgcaat ttgggacaaa ccgacccggc atgaagacat tgtcgccgtt
      121 ttccctttcg gcaaccccat acacaacaac acgatgatta tgaggcgcag cgtcattgac
      181 ggcggtttgc ggttcgatcc agcctatatc cacgccgaag actataagtt ttggtacgaa
      241 gccggcaaac tgggcaggct ggcttattat cccgaagcct tggtcaaata ccgcttccat
      301 caagaccaga cttcttccaa atacaacctg caacagcgca ggacggcgtg gaaaatcaaa
      361 gaagaaatca gggcggggta ttggaaggcg gcaggcatat ccgtcggggc ggactgcctg
      421 aattacgggc ttttgaaatc aacggcatat gcgttgtacg aaaaagcctt gtccggacag
      481 gatatcggat gcctccgcct gttcctgtac gaatatttct tgtcgttgga aaagtattct
      541 ttgaccgatt tgctggattt cttgacagac cgcgtgatga ggaagctgtt tgccgcaccg
      601 caatatagga aaatcctgaa aaaaatgtta cgcccttgga aataccgcag ctattgaaac
      661 cgaacaggat aaatcatgca aaaccacgtt atcagcttgg cttccgccgc agagcgcagg
      721 gcgcacattg ccgatacctt cggcagtcgc ggcatcccgt tccagttttt cgacgcactg
      781 atgccgtctg aaaggctgga acaggcgatg gcggaactcg tccccggctt gtcggcgcac
      841 ccctatttga gcggagtgga aaaagcctgc tttatgagcc acgccgtatt gtggaaacag
      901 gcattggacg aaggcttacc gtatgttgcc gtatttgagg atgatgttct gcttggcaaa
      961 gacgcagaaa agttccttgc cgaagatact tggttggaag agcgttttga taaggattcc
     1021 gcctttatcg tccgtttgga aacgatgttt gcgaaagtta ttgtcagacc ggataaagtc
     1081 ctgaattatg aaaaccggtc atttcctttg ctggagagcg aacattgtgg gacggctggc
     1141 tatatcattt cgcgtgaggc gatgcggttt ttcttggaca ggtttgccgt tttgccggca
     1201 gagcggatta aagcggtaga tttgatgatg tttacttatt tctttgataa ggaggggatg
     1261 cctgtttatc aggttagtcc cgccttatgt acccaagaat tgcattatgc caagtttctc
     1321 agtcaaaaca gtatgttggg tagcgatttg gaaaaagata gggaacaagg aagaagacac
     1381 cgccgttcgt tgaaggtgat gtttgacttg aagcgtgctt tgggtaaatt cggtagggaa
     1441 aagaagaaaa gaatggagag tcaaaggcag gcggagcttg agaaagttta cggcaggcgg
     1501 gtcatattgt tcaaatagtt tgtgtaaaat ataggggatt aaaatcagaa atggacacac
     1561 tgtcattccc gcgcaggcgg gaatccagtc cgttcggttt cgcttgtttt aagtttcggg
     1621 taacttccac ttcgtcattc ccgcgaaagt gggaatccgg ttcgttgagt ttcagtcatt
     1681 tccgataaat caccgcaacg ttaaatttct agattcccgc ctgcgcggga atgacggcgg
     1741 agagggtttg ttgtttcggg taaatccctg tagtttttcg tttccagatt cccgctttcg
     1801 cggggatgac ggcgggggga ttgttgcttt ttcggataaa atcccgtgtt ttttcatctg
     1861 ctaggtaaaa tcgccccaaa gcgtctgcat cgcggcgatg gcggcgagtg gggcggtttc
     1921 tgtgcgtaaa atccgttttc cgagtgtaac cgcctgaaag ccggcttcaa atgcctgttg
     1981 ttcttcctgt tctgtccagc cgccttcggg cccgaccata aagacgattg cgccggacgg
     2041 gtggcggatg tcgccgagtt tgcaggcgcg gttgatgctc ataatcagcc tggtgctttc
     2101 agacggcatt ttgtcgagtg cttcacggta gccgatgatg ggcagtacgg ggggaacggt
     2161 gttcctgccg ctttgttcgc acgcggagat gacgatttcc tgccagcgtg cgaggcgttt
     2221 ggcggcgcgt tctccgtcga ggcggacgat gcagcgttcg ctgatgacgg gctgtatggc
     2281 ggttacgccg agttcgacgc ttttttgcag ggtgaaatcc atgcgatcgc cggaggaaat
     2341 ggattggatg agcgtgatgt tgagcgggga ctcgttgtcg gttgtgtctt cgtgcaggat
     2401 ttcggcttcg gcgcggtgtt tttccaaaac ggtcagccgt gcggtatgtg ccttgccttt
     2461 gccgtcgaag agggtgatgt tttcgttggg gcggacgcgc aggacgttga ggtggcggac
     2521 gatgttgtcg ggcagggcga cggtttgtcc gacggaaagg ttttcgggca ggtagaatct
     2581 gggcatagtt tttggcggtt cggacggtta taatccgtcg cagcggcgtg atggaaacgg
     2641 acattatacg ttcgcgcgcc gcaagttcaa aatatgagga cagtaggaag tgttacaccg
     2701 tttgcagaaa gtcgtgcgcc atatcgcgca aaccgaaatt atgccgcgtt ttttgaatac
     2761 gccgtcccgc cgcaaggaag acggttcgat gttgagcgag gcggacattg ccgcgcagac
     2821 ggcatttgcc gccgcgttgc cgcttctgat cgattgcccg atgttgggcg aggaaatgtc
     2881 gcggcaggaa cagtcggctt tgtgggaaca atattcgggg gaaaaggggc tgtggattgt
     2941 cgatccgata gacgggacga acaattttgt caacgggctg ccgcattttg ccgtgtcggt
     3001 ggcgtttgtc cgcaacgggc gcgccgaatt gggcgtaatc tacaatccgg tcagcggcga
     3061 atgtttttat gccgaacgcg ggcagggtgc gtttcttaat gggacacgcc tgcctttgcg
     3121 tctcgtggat aaaaaactca atgaggcgat tgcgggcgtg gaaatcaaat atctgcgttc
     3181 gggcaaactt tccagccgca tgagtacgct cgcgcctttc gggacgatac gcagtatggg
     3241 cagcagcacg ctggactggt gttatctggc gtgcgggcgt tatgatgttt atgtccacgg
     3301 cgggcagaag ctgtgggatt atgctgcggg tgcgttgatt ttcgaggagg cgggcggcag
     3361 gctgacgact ttggaaggcg acgggttttg gagtggtgaa cacgtgttca aacgctcggt
     3421 cgtcgccgcg ctcgaaccga agctgtttga acgctgggtc gggtggatac gggaaa
//
LOCUS       DAXFZR010000128         3452 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000128 DAXFZR010000000
VERSION     DAXFZR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.129"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(457..846)
                     /locus_tag="ACFJAS_002061"
     CDS             complement(457..846)
                     /locus_tag="ACFJAS_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC3983217.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQPAR"
     gene            complement(1009..1388)
                     /locus_tag="ACFJAS_002062"
                     /pseudo
     CDS             complement(1009..1388)
                     /locus_tag="ACFJAS_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1442..2575)
                     /gene="carA"
                     /locus_tag="ACFJAS_002063"
     CDS             complement(1442..2575)
                     /gene="carA"
                     /locus_tag="ACFJAS_002063"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC3983218.1"
                     /translation="MNTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          740 a          892 c          954 g          866 t
ORIGIN      
        1 ctcgctagct ctctcccaca gggagagagg acgggtcggc tgttggggtt gaggtttgct
       61 gcaaggaaaa caggttgtgc aggctgcttt ttatttttca gacgacctga aacaacagct
      121 gcaaatctaa gttgcctttc aaatcgcctg caaccgaaaa atctgccgaa acccactccc
      181 tcccctgtgg gggagggccg gggagagggc attctccaag ttgcggcaac ctttcccaac
      241 aactcaaccg ccccaataca agccttgcgg cttgttgccc tctctccagc cctctcccac
      301 agggagagag gacgggtcgg ctgttggggt tgaggtttgc tgcaaggaaa acaggttatt
      361 caggttgctt tttatttttc agacgacctt tttaaacgct taattcagtt aattttacct
      421 tattattttc aaaatctaat caaaatcaac cgtttgctac cgtgctggct gcttttccaa
      481 ttcctgcaat acgcgcagga tttccgccag tacatcgttt gtctgctgca aaatttcgtg
      541 attccaaaaa cgcagcacgg taaagcccaa gctgttgaga tataccgtcc gcgcgtggtc
      601 gtatacggct tgttccgcgt gctgcccgcc gtctgcttcg acaatcagct tgggcgttac
      661 gcacataaaa tcaacaatat aattccccat cggctgctgt cggcggaatt tatagccgtt
      721 cagacggcct gcccgcaggt gttgccacaa ttttgcttcc gcctcgctca tttcttgacg
      781 catggctttg gcgcgttggc gcagggcggg gttttcggcg gtcaatagtt tctcgggcgg
      841 gttcattttt gttatcgggt tgctgtttgt gtaagcctta cagcttgttg ccctctctct
      901 aactctttcc cacagggaga ggggacgggt cggctgttgg ggttaaaggg gctgcaagga
      961 aaacagcttg tgcaggctgt tttttatttt gcataatccg tctgaaaatt aataactgct
     1021 gatttccagc aggttgccat cgggatcgcg caggtaaacc gattggattt tgcccattgc
     1081 gcctgtgcgc gctacgatgc cgcttaaagg tttgatgccg tgtgcggata attcctgtaa
     1141 aaccgtttcc agtggggcgt atcggtcagc aggcataaat ccgctgtgcc gcaggcggcg
     1201 tgttgcgcgt taggctgaat ttccgcaccg cgcccgtgta ggttgatttt ctgactgcca
     1261 aacaacaaag ctttacggtt gttgccaaat gaaacttctt ccatgcccaa aacttgtgtg
     1321 taaaacgcga tggttcggtc aatgtcggca acagtcagta ctagatggtc gagtgcgcta
     1381 attttcattt ccagctttct ttttcagacg gcagcagcat actgttgccg tctgaaaatc
     1441 attatgcttg ttttgcagcc ttcatattgc caatgaattt gtcgaacaaa tagccgacat
     1501 cttgcggacc cgggctggct tcggggtgtc cttggaaaca gaacacgggt ttgtcggtca
     1561 gctcgatgcc ttgcaaggta ttgtcgaaca aggatttgtg ggtaatgcgt gcgttggcgg
     1621 gcagggtgtc ggcatcgacg gcgaaaccgt ggttttggct ggtaatgacg actttgccgc
     1681 tgtccaaatc ttgcacagga tggttcgcac cgtggtggct gaagcgcatt ttcagggttt
     1741 tcgcgccgat ggcgaggctg atgagctggt gtcccaagca gatgccgaaa atcggtttgc
     1801 cgctttccat cagtttttgc acggcttcga tggcgtaggt gcaaggctcg gggtcgccgg
     1861 ggccgttgga caggaacacg ccgtcgggat tgagtgccaa cacgtcttcc gcgctcgttt
     1921 gtgcggggac gacggtcagg cggcagccgc gcgaggcgag catacgcagg atgttggttt
     1981 tcacgccgaa atcgtaggcg acgacgtggt aaggctgttt gtcaggggta acgaaacctt
     2041 tgcccaattc ccattcgcct tccgtccatt cgtaagtttc cgtgcaggaa acttcttttg
     2101 ccaaatcttt gccgaccatg ctgccgaacg cggcgatgag ttcttgcgct ttttcaacgg
     2161 tggcatccgc acctgtcaga atcgcgccgc cttgcgcgcc tttttcgcgc aacagcatgg
     2221 tcaggcggcg ggtgtcgatg tcggcgatgg cgacggtttc gttgcgtacc aaatagtcgt
     2281 gcaggctttc ggaggcgcgg aagttgctgt gcaagagcgg caggtcgcgg ataatcaggc
     2341 cggcggcata aacgctgcgg ctttcttcat cttcggcgtt ggtgccggta ttgccgatgt
     2401 gcggataggt gagggtaacg atttgtttgc agtaggacgg atcggtcagg atttcctgat
     2461 agccggtcat cgaagtattg aacacgactt cgccggaagc cgaaccttcg taaccgattg
     2521 atgtgccgtg gaatacgctg ccgtcagcga ggacgaggag ggcgggggtg ttcatgatgg
     2581 gaatcctgtt tttaataaaa ttcttgacaa tgccgtctga agggacttcg gcagggggca
     2641 tccggtcgga aaaatccggt caaaaaaaaa cacgccgcgc aagatagacg cgtaacgtgc
     2701 ttttcgggca agtgcctata ccctgaaagc agcatatttt aagacgaaac atacccgatt
     2761 tcaagcgcaa acgcggcagg cgggctgata tgttcgcgcg aaaaccgaaa tccggggcat
     2821 aaaaccgccc gctccgcgcc ccctatgccc gttacgcatt tccccacgtc cgcccggcga
     2881 aactatggga atacccgaac cgtcattccc gcgcaggcgg gaatccggac ttgtcggcac
     2941 ggaaacttat cgggtacaac ggtttcttga gattttacgt cctagattcc cgcctgcgcg
     3001 ggaatgacgg catcggtttg acggtattta attgaattgt ggaaattgac ggattcagtg
     3061 agattggcgg gatgaagcct accctgtagc ccgcctttta tagcggatta acaaaaaccg
     3121 gtacggcgtt gccccgcccc ggctcaaagg gaacggttcc ctaaggcgcc caagcaccgg
     3181 gcgaaccggt tccgtaccat tcgtactgcc tgcggcccgc cgccttgtcc tgatttttgt
     3241 taatccgcta tacggacccg ccccctcccg aaaaacgcaa aaaatgccgt ccgaaaacct
     3301 ttcggacggc attttcgcgt gaaccgtcat tcccacgaaa acagaaaacc aaaatcagaa
     3361 acctaaaatt cgtcattccc acgaaagtgg gaatccagga ctcaggacct gagaaacctt
     3421 tttacccgat aagttcccgt accgaccggt cc
//
LOCUS       DAXFZR010000129         3445 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000129 DAXFZR010000000
VERSION     DAXFZR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.130"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(227..442)
                     /locus_tag="ACFJAS_002064"
     CDS             complement(227..442)
                     /locus_tag="ACFJAS_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983219.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(473..3241)
                     /gene="tdfH"
                     /locus_tag="ACFJAS_002065"
     CDS             complement(473..3241)
                     /gene="tdfH"
                     /locus_tag="ACFJAS_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC3983220.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAERVPKDKKVFTDARAVSTRQDVFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSNKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNAGSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEEHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTRIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSENEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSDVRQLGKRSIKQTETLARQPLIFDFYAAYEQKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNDDNTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          788 a          862 c          858 g          937 t
ORIGIN      
        1 cggtaatgat gagattttag gttgtgggat ttatcggaaa aacagaaacc tctccgccgc
       61 cattctcacg aaagtgggaa tccggaaagc taaagccaca ggaatttatc ggaaatggct
      121 gaaatttata gtggattaac aaaaatcagg acaaggcgac gaagctgcag acagtacaaa
      181 tagtacggca aggcgaggca acgccgtacc tgtttttgtt aatccactat aaaaaaactg
      241 attcccgctt ttgcgggaat gacgactcat aaattacccg aaacaaccaa aaataaccga
      301 aatcaaaaac cggcttccct gcgtgggaat gaaatggatt tttctttgag ttgccgatgc
      361 gataaaacgg attggcatag gcttgcggca ggatgatgcg tttggggaaa caaatgccgt
      421 ctgaagggct ttcagacggc attgcagttt ttccacaaaa cgcgggctgc ctttaaaact
      481 tgtagctcat cgtcatcaag aaggtgcgtc cgcgtgcgaa attggtcaat acgcttttgc
      541 ttgtgccgcc gtatttgccg ttgcataacg tgttatcatc attacacgtt acgtcttcgt
      601 ccttgtcttt cgggtcgaac gagctgtaat aacgctgcgt tgccgcatca ttgcccgcat
      661 cgagcggatc gatataacgc ctgtcgaaca gatttttgac ttcggcgcgg aaaataaggt
      721 ttttcttctg ctcgtaagcg gcgtaaaaat caaaaatcaa aggctgtcgg gcaagggttt
      781 cggtttgttt gatggaacgc ttgcccagtt gccggacatc gctggtattt cccccgttgg
      841 tgccgtcgat atagcgttct tcagccgtcg cgcggatgct cttgccgaaa tagcgcatcg
      901 cgccgcccaa agtcagtttg ttgcccaacc agcgcgtacc gacttccaaa cgtccgtaat
      961 ctcgcggcag ggcggaaacc ctgctcaacc cataaccttg tttgagttgg tcttctttgg
     1021 acgcattgtt gggcgattcg ctcgcatcgc tgaaattggt cggttgcgtg cttttttgat
     1081 aggcgtaaga aaggttggtg aaaaaacgcc cataatcgta attcagctcc agctcaaaac
     1141 cgtgtttgtg cactttgtct ttgaaattgc ggtgtcggat ggtgtaggca agcccggtgc
     1201 tgccgaccca gctcggaata tccccgttca aatcccacca tttcccgtaa acgttgtgga
     1261 tgtagttgtc aatgcggctg cggtagccga ccagtttcaa tcctaatata tcatcttgtt
     1321 ttaacaatcc ttttttatag gtattgaagc caaattgcca agtgtttgcg cgctctggtt
     1381 ttaaggcggt gtgaacgccg gagtcgccga tttgggaaaa atacatttct tggatgttgg
     1441 gcatacggtg tgtgcgcgaa tagccggcga acggcatgaa ataatcgccg aagtccgcac
     1501 taatgctgac cgaatggttg ttggcgcgct ttttgccgta ttttttcaat acgggttcat
     1561 aaagcccgca gctcgggtcg caatgttcct tgtatgccgg cgagttttct ccgaatgccc
     1621 gcttaaattc gttttccgag ccgtaatagc ccgtatattc gccgccgaaa cggtagttga
     1681 ttgcattggt gctgtagttt aagcggtaaa tgtctttttt gagcgcggca tcgaagtaga
     1741 acgtgttgaa atattgactg ccggccggtt ggacgatggt tgatttttgg ggcagcagcc
     1801 ctttatcgcc cttaaaccgc cccaaatagg aataaagccc gttgtcctga tccggaccgt
     1861 cgaaaaacag ccccaattct tcaggaaagc ggtttttgcc gtattcgttg tggaaataat
     1921 tgaagcccaa agtggtttgc aactcggttt cgcggggcag ccggaaggtg gcggtgttgt
     1981 tgaggtcgag gattttcgcg ttgttgtagg tttcaaaatc ctttaaaagc ccccagcctg
     2041 taaacttcga ccctttcgga tatttctgcc tgcccgaatt gtaggctgcg gtcagattga
     2101 ggttggtata cgggttcaaa gacaaaccgt aattgaattg ataattgcgg ttgatgattt
     2161 tgcggctgcc gattctggtg tttaaatcgc gaaattgcgc cgtgtattta ttgaatacgc
     2221 cgtcgtattc caatttaaac agattgcctg ccgactgctg cttcaggccg gacggatcga
     2281 tgggggtgat gtcgtattgc ggcgccaggt tttcccgcca gcttttatca tgctcttcga
     2341 tgtatttttg cagttcttgg gggtcttcgt attttttata ccactttgtt ttccagtatt
     2401 gcctttgcaa atcccgttcc cattttccgc tgccggcatt gaatttcaaa ccaccctctt
     2461 gtacaaaata ttgctgtttg cgccgttcca gatattcttc accaaaattt ccgatgtgct
     2521 gcccgccgcc gcccacgcgg taattttgcg ccacgccgcg cctgctgtgc ccgtaaagca
     2581 caccgacaga cgctccgctt tccagccatt tgcgcgcacc tatcgccgcc atcgcattgc
     2641 ctttgtttga attggtgccg gtcagacctt ttagcagcag gccgtaggta ttattgccct
     2701 gaacgacgtc atccacgcct aaagtccgca gattcgccga accggcaagg ctgttgatgc
     2761 ctgccgagcc gctgaagctg cctttgacga catccagtcc ggcaataaaa ttgctgtcga
     2821 cagatgcacc gaattgagat gaaccgcctg ccctgcccgc atcggtagaa gtcgaataaa
     2881 aggtctgcgt gatgccgtcc accatcgtat tgacccgccc gaacccgctg tcgccgcgaa
     2941 tattcaaaga cacaatgccc gagcttttat cttgctgtgt aaacgcaccg ggtatgctgc
     3001 gtacgatgtt gtcgaggttt tcgccggatt tgaacacatc ctgacgggtc gatacggcac
     3061 gcgcatcggt aaacactttt ttgtctttcg gtacgcgctc agccttgacg tgcacgtctt
     3121 ccaaaacctg tatctgcgcc tcgctgcccg cgcgccctgc atcttcggca taactatgat
     3181 gatatagcat aacacccata agataaaaac aaatcggctt caaccggaaa gaagatctca
     3241 tattttcctc aacaataaac aatcaatcca acatgtctac cgttttcatc gaatccgccc
     3301 tttcgaccac ccggccctac gcaaccgaac cgtcattccc acggaagtgg gaatctagga
     3361 cgcggggttt gggcaaccgt tttatccgat aagtttccgt gcggacaggt ccggatttcc
     3421 gcctgcgcgg gaatgacggg tttcg
//
LOCUS       DAXFZR010000130         3444 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000130 DAXFZR010000000
VERSION     DAXFZR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.131"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            98..3298
                     /gene="nalP"
                     /locus_tag="ACFJAS_002066"
                     /pseudo
     CDS             98..3298
                     /gene="nalP"
                     /locus_tag="ACFJAS_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
BASE COUNT          928 a          941 c          930 g          645 t
ORIGIN      
        1 gatataattc aaaatataaa aatgggttac atctggacat tacgaaattt ttattccttc
       61 gcctgaattc tattgtcaga ttcaaagaga cctcatcatg cgaaccacct caaccttccc
      121 tacaaaaact ttcaaaccgg ctgccatggc gttagctgtt gcaacaacac tttctgcctg
      181 cttaggcggc ggcggaggcg gcacttctgc tcccgacttc aatgcaggcg gcaccggtat
      241 cggcagcaac agcagggcaa cgatagcgga atcagcagca gtatcttacg ccggtataaa
      301 aaacgaaatg tgcaaagaca gaagcatgct ctgtgccgat cgggatgacg ttgcggttac
      361 agacagggat gccaaaatca aagccccccg aatctgcata ccggagactt ttcaaaccca
      421 aatgaccaat attaagaata tgatcaacct caaacctgca attgaagcag gctatacagg
      481 acgcggggta gaggtaggta tcgtcgatac aggcgaatcc gtcggcagca tatcctttcc
      541 cgaactgtat ggcagaaaag aacacggcta taacgaaaat tacaaaaaca aattacaaaa
      601 actatacggc gtatatgcgg aaggaagcgc ctgaagacag aggcggtaaa gacattgaag
      661 cttctttgga caatgaggcc gttatagaga ctgaagcaaa gccgacggat atccgccatg
      721 taaaagaaat cggacacatc gatgtggtct cccatattat tggcgggcgt tccgtggacg
      781 gcagacctgc aggcggtatt gcgcccgatg cgacgctaca cataatgaat acgcatgatg
      841 gaaccaagaa cgagaaataa tgtctgcagc catccgcaat gcatgggtca agctgggcga
      901 acgtggcgtg cgcgtcgtca ataacagttt tggaacaaca tcgagggcag gcactgccga
      961 ccatttccaa atagccaatt cggaggagca gtaccgccaa acgttgctcg actattccgg
     1021 cggtgataaa acagatgagg gtatccgcct gatgcaacag agcgattagg gcaacctgtc
     1081 ctaccacatc cgtaataaaa acatcctttt catcttttcg acaggcaatg acacacaagc
     1141 tcagcccaac acactgaccc tattgccatt ttatgaaaag acgctcaaaa aggcattatc
     1201 acagtcgcag gcgtagaccg cagtggagaa aagttcaatg gctccaacca ttgcggaatt
     1261 actgccatgt ggcgcctgtc ggcatcctat gaagcaaccg tccatttcac ccgtacaaga
     1321 ccgattcaag ttgccggaac atccttttcc gcacccatcg taaccggcac ggcggctctg
     1381 ctgcagagat acccgtggat gagcaacgac aacctgcgta ccacgctgct gacgacggct
     1441 caggacatcg gtgcagtcgg agtggacagc aagttcggct gggggctgct ggatgcgggt
     1501 aaggccatga acggacccgc gtcctttccg ttcggcgact ttaccgccga tacgaaaggt
     1561 acatccgata ttgcctactc cttccgtaac gacatttcag gcacgggcgg tctgatcaaa
     1621 aaaggcggca gccaactgca actgcacggc aacaacacct atacgggcaa aaccgttatc
     1681 gaaggcggtt cgctggtgtt atcggatatg cacgtcaaaa ccaaaggtgc actgatttat
     1741 aacggggcgg catccggcgg cagcctgaac agccacggca ttgtctatct ggcagatacc
     1801 gaccaatccg gcgcaaacga aaccgtacac atcaaaaggc ggtctgcagc tgggcgacaa
     1861 aggtacgctg tacacacgtt tgggcaaact gctgaaagtg gacggtacgg cgattaccga
     1921 cggcaagctg tacatgtcgg cacgcggcaa gggggcaggc tatctcaaca gtaccggacg
     1981 acgtgttccc ttcctgagtg tcgccaaaat cgggcaggat tattctttct tcaaaaatat
     2041 cgaaaccgac ggcggtctgc tggcttccct cgacagcgtc gaaaaaacag cgggcagtga
     2101 aggcgacacg ccgtcctatt atgtccgtcg cggcaatgcg gcacggactg cttcggcagc
     2161 ggcacattcc gcgcccgccg gtctgaaaca cgccgtagaa cagggcggca gcaatctgga
     2221 aaacctgatg gtcgaactgg atgcctccga atcatccgca acacccgata cggttgaaac
     2281 tgcggtcgcc gaccgcacag atatgccggg catccgccta cggcgcacaa ctttccgcgc
     2341 agcggcagcc gtacagcatg cgaataccgc cgacggcgta cgcatcttca acagtctcgc
     2401 cgctaccgtc tatgccgaca gtgccgccgc ccatgccgat atgcagggac gccgcctgaa
     2461 agccgtatcg gacgggttgg accacaacgg tacgggtctg cgcgtcatcg cgcaaaccca
     2521 acaggacggt ggaacgtggg aacagggcgg tgtcgaaggc aaaatgcgcg gcagtaccca
     2581 aactatcggc attgccgcga aaaccggcga aaatacgaca gcagccgcca cactgggcat
     2641 aggacgcagc acatggagcg aaaacagtgc aaatgcaaaa accgacagca ttagtctgtt
     2701 tgcaggcata cggcacgatg tgggcgatat cggctatctc aaaggcctgt tctcctacgg
     2761 acgctacaaa aacagcatca gccgcagcac cggtgcggat gaatatgcgg aaggcagcgt
     2821 caacggcacg ctgatgcagc tgggcgcact gggtggtgtc aacgttccgt ttgccgcaac
     2881 aggagatttg acggttgaag gcggtctgcg ccacgacctg ctcaaacagg atgcattcgc
     2941 cgaaaaaggc agtgctttgg gctggagcgg caacagcctc actgaaggca cactggtcgg
     3001 actcgcgggt ctgaagctgt cgcaaccctt gagcgataaa gccgtcctgt ctgcgacggc
     3061 gggcgtggaa cgcgacctga acggacgcga ctacgcggta acgggcggct ttaccggcgc
     3121 ggctgcagca accggcaaga cgggtgcacg caatatgccg cacacccgcc gggttgccgg
     3181 tctgggggtg gatgtcgaat tcggcaacgg ctggaacggc ttggcacgtt acagctacac
     3241 cggttccaaa cagtacggca accacagcgg acaaatcggc gtaggctacc ggttctgacg
     3301 gacagaaaac agacagccgc aaagatcacg gtctttgcgg ctgtttctta tgaaaagaaa
     3361 accctattcc aattgcctgc ttctattgtt tcaagacttc ttccaaagat tcggcatcaa
     3421 tcagacgtat agcggattaa caaa
//
LOCUS       DAXFZR010000131         3350 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000131 DAXFZR010000000
VERSION     DAXFZR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.132"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..132
                     /locus_tag="ACFJAS_002067"
     CDS             <1..132
                     /locus_tag="ACFJAS_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3983221.1"
                     /translation="KPSKKWQARIMVNRKNKILGLFEHIEDAVKAREAASKDFGFVV"
     gene            197..619
                     /locus_tag="ACFJAS_002068"
     CDS             197..619
                     /locus_tag="ACFJAS_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983222.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDL"
     gene            619..1158
                     /locus_tag="ACFJAS_002069"
     CDS             619..1158
                     /locus_tag="ACFJAS_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983223.1"
                     /translation="MPRLSDEDWRKVELDYRRGVLSIAEIGRKYNVSAQHVGRVAKER
                     VWTRDLNDEVQAKARAMVLSADKNGNAPDYADFNLKQVTDAEAKRVAAVQRRHRNLAE
                     NLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIERKSYGMDTAESKISESA
                     KTAGIRIEFVGPEDDGKDG"
     gene            1139..2413
                     /locus_tag="ACFJAS_002070"
     CDS             1139..2413
                     /locus_tag="ACFJAS_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFC3983224.1"
                     /translation="MTGKTVDLKLPAKLDGLFKPCRYKVMYGGRGGGKSHGAASALLA
                     LGAQRPLRILCAREIQKSMRDSVHRLLKDKVAQLGLGHFYEITDFEIRGANGTLFVFS
                     GLQSHTVDSIKSFEGIDIVWVEEGHGVSKKSWDVLTPTIRKEGSEIWITLNPDMETDE
                     TYRRFIAMPSEDTWLCEINWRDNPWFPEALNRERLKAQRSMSKEDYGNIWEGRPRMVS
                     EGAVYRHEIQDAFHSGRVTLVPYDSSLPVHTVWDLGWNDAMTIGLVQRDLTSVRIIGY
                     IEDTHRTLDWYVAELEKLPYRWGTDFLPHDGRTRNFQTGKSTMEILTGLGRKSVFVQN
                     ATGIEEGIRAARMLFPKVYFDKDKTARLLECLKRYGRQIHAKTGVAMGPLHDEYSHGA
                     DMFRYLAQAVDLMDTGSNTGYTETPVSDWRLY"
     gene            2398..>3350
                     /locus_tag="ACFJAS_002071"
     CDS             2398..>3350
                     /locus_tag="ACFJAS_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983225.1"
                     /translation="MEALLMGTDVPETGVLPDKNGEPLTIGEYRLFVGEMMNQPAWRA
                     VADKEMDYADGRQLDNELLQKQRELGLPPAVENLITPTLLSVQGYEATIRTDWRVTAD
                     GETGGRDVADALNFKLNRAERQSRADKACSDAFRGQIACGIGWVEVTRNPNPFEFPYE
                     CGVIHRNAIHWDMKSYKYDLSDARWLIRRRWLLPERLAQFFPEYAGHFKAMGRGGSDW
                     RISGEMLDGGGNTGLADAWGISGRNTVSEEFWFNETTRELAVAEVWYRRWVTADCLRD
                     KKTGRTVEFDGANPNHREMAANGAVLFAASVPRMRRAFVVG"
BASE COUNT          805 a          793 c         1073 g          679 t
ORIGIN      
        1 aagccgtcta agaaatggca agctaggatt atggttaata gaaaaaataa aatattaggt
       61 ttatttgaac atattgaaga tgcagtgaaa gcgagagagg cagcatctaa agattttggc
      121 tttgtagtgt aaccgcctgc aatcgcgggc ggcggcggag tttggaatcg tttgggatgt
      181 ttaggagttt gatttgatgt cgggattgtc ggatattgac aaggtgtacc agggggttgt
      241 ggatttgcac aacgcggagc aggtggtcag ccgcgaggct ttggcggaaa cgacaggcct
      301 gaaaatgtcg gaaatcacta aatttaccaa gctgctggtc gagcacggca aaatctaccg
      361 cgtgacaagg gggattttca agcccgccat agggtttggc gagacgcgcc ccgtcagcgt
      421 atcggtattg gattcgggga tgggcgtatt ggaaataggc gatacggtat tgcacctcaa
      481 cccgcaggaa atgcgctcgt tgggggcttt gatgtcgggg ttcgggcagc agttttccag
      541 tattcagatg gggcgcgagt tttcagtatt gcggaattat ctggaatgtt ccgccaaaaa
      601 cgggaggttg gacctttaat gccgagattg tcagatgaag attggcgcaa agtcgagctg
      661 gattaccggc gcggggtttt gagcattgcc gaaatcggcc gcaaatacaa tgtttcggcg
      721 caacacgtcg gcagggttgc caaagaacgc gtgtggacgc gcgatttgaa tgacgaggta
      781 caggcaaagg cgcgggcgat ggtgttgtcc gcggacaaaa acggcaatgc gccggattat
      841 gcggatttca accttaaaca agtaacggac gcggaagcga aacgggttgc cgccgtacag
      901 cgcaggcatc ggaatttggc ggaaaacctg tccaaaagcg cggagcagat tgtcgcggaa
      961 ttgtacggaa gcccggagga cacattcaca aaggcgcgta tgttccaaac ggtggcggcg
     1021 gggtttaagg ttttggtgga aatcgagcgt aaatcatacg gtatggatac cgcggaatcg
     1081 aagatttcgg aatccgccaa gacggcgggc atccgtatcg aatttgtagg gcctgaagat
     1141 gacgggaaag acggttgatt tgaaattgcc cgcaaaactg gacgggctgt tcaagccttg
     1201 ccggtacaag gttatgtacg gcgggcgcgg cggcggcaaa tcgcacggcg cggcatccgc
     1261 actgctggcg ctgggggcgc aacgcccttt gcgtatttta tgcgcgcgcg aaattcaaaa
     1321 atcgatgcgc gattccgtac accgcctgtt gaaagacaaa gtggcgcagt tgggtttggg
     1381 gcatttctac gaaataaccg acttcgagat acgcggcgca aacggcacgc tgttcgtgtt
     1441 ttcgggcctg cagtcgcata ccgtggacag catcaaatcg tttgaaggta tcgacatcgt
     1501 atgggttgag gaagggcacg gcgtcagtaa aaaaagctgg gacgtgctca cgccgaccat
     1561 acgcaaagaa ggttcggaaa tttggattac cctcaatccc gatatggaga cggacgaaac
     1621 ctaccggcgt tttatcgcta tgccgtccga agacacttgg ctttgcgaaa tcaactggcg
     1681 cgacaatccg tggttccccg aagcattgaa ccgggagcgg ctcaaagcac agcgttcgat
     1741 gagtaaagag gactacggga atatttggga aggcaggccg cgcatggtat cggagggggc
     1801 ggtttaccgg catgaaatac aggacgcttt tcattccgga cgcgttacac tcgtccctta
     1861 tgattcttct ttgcccgtgc atacggtttg ggatttgggc tggaacgatg ccatgaccat
     1921 cgggctggtg cagcgcgatt tgacgagcgt gcgcatcatc ggctacatcg aagacacgca
     1981 ccggacgttg gactggtatg ttgccgaatt ggaaaagctg ccctaccggt gggggacgga
     2041 cttcctgccg cacgacggca ggacgcgcaa cttccaaaca ggcaaaagta cgatggagat
     2101 tttgaccgga ctggggcgca agtcggtttt cgtgcaaaac gcgaccggta tcgaagaagg
     2161 catcagggcg gcgcggatgc tgtttcccaa agtgtacttc gataaagaca aaacagcgcg
     2221 gcttttggaa tgcctgaaac ggtacggccg ccaaatacat gcgaaaacag gcgtggcaat
     2281 ggggccgctg cacgacgaat attcgcacgg cgcggatatg ttccgctacc tggcgcaggc
     2341 ggttgattta atggatacag gcagcaatac gggatacacg gaaacgcccg tttcggattg
     2401 gaggctttat tgatggggac ggacgtaccg gaaacaggcg tattgcccga taaaaacggc
     2461 gaaccgctga ctatcgggga ataccggctg tttgtcggtg aaatgatgaa ccaacctgca
     2521 tggcgtgccg ttgccgacaa ggaaatggac tacgccgacg gcaggcagct tgacaacgag
     2581 cttttacaga aacagcgcga gttgggcctg ccccccgccg ttgaaaacct gattaccccg
     2641 accctgctgt cggtacaggg atatgaggcg acgatacgga cggactggcg cgtgacggcg
     2701 gacggcgaaa ccggcgggcg ggacgtggcg gacgcattga acttcaaact caaccgcgcg
     2761 gaacggcaaa gccgtgccga caaggcttgt tcggacgcgt tcagggggca gatagcctgc
     2821 ggcatcggct gggtggaggt tacacgcaac cccaaccctt tcgagtttcc ttatgagtgc
     2881 ggcgtcatcc accgcaacgc catccattgg gatatgaaat cttacaaata cgacctgtcc
     2941 gatgcccgct ggctgatacg ccgccgctgg ctgctgccgg aacgcctggc gcaattcttc
     3001 cctgaatatg ccggacactt caaagcgatg gggcgcggcg gttcggactg gcgcatcagc
     3061 ggggaaatgc ttgacggcgg cggcaatacc ggactggcgg acgcttgggg tatttcgggg
     3121 cgcaacaccg tcagcgaaga gttttggttc aatgaaacca cgcgcgaact ggcggtggcg
     3181 gaagtatggt acaggcggtg ggtaacggca gactgcctgc gcgacaaaaa aacagggcgc
     3241 acggtggagt ttgacggcgc aaacccaaac catcgggaga tggcggcaaa cggcgcggta
     3301 ttgtttgccg cttccgtccc gcgtatgcgc cgcgcctttg tcgtggggga
//
LOCUS       DAXFZR010000132         3270 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000132 DAXFZR010000000
VERSION     DAXFZR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.133"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(35..772)
                     /gene="ubiE"
                     /locus_tag="ACFJAS_002072"
     CDS             complement(35..772)
                     /gene="ubiE"
                     /locus_tag="ACFJAS_002072"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC3983226.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(815..1183)
                     /locus_tag="ACFJAS_002073"
     CDS             complement(815..1183)
                     /locus_tag="ACFJAS_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC3983227.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            1303..2922
                     /gene="mnmC"
                     /locus_tag="ACFJAS_002074"
     CDS             1303..2922
                     /gene="mnmC"
                     /locus_tag="ACFJAS_002074"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC3983228.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
BASE COUNT          837 a         1092 c          661 g          680 t
ORIGIN      
        1 tgttcagacg gcattggctt cacaggcagt ccgtttaaaa tttcacgccc ttatgcagcg
       61 cgacgatgcc cgcactcata ttgtgataat ccacgctgtc gaagcctgca tccagcatca
      121 tctgtttcaa agtttcctga tcggggtgca tacggatgga ttcggcgaga tactggtagc
      181 tgtccgcatc tttggcaatc agcctgccca tcaccggcaa cagcttgaaa gaataaaaat
      241 cgtatgcgcc ttcgagcggc ttgtagattt tggaaaactc caacaccagc aacgtaccgc
      301 ccggtttcaa aacacggtac atttccttaa gcgcggcatc tttatacgtc atgttccgca
      361 agccgaacgc cacggagacc aagttgaaat aattgtcggg gaaaggcagt ttttccgcat
      421 cggcaagcga cacgggtaaa atcatgcctt cgttcaacag acggtcgcgc ccgacggtca
      481 gcatagaaga attaatatcg gtcagccaaa cctcgccttc cttgccgacc cgtttcgccc
      541 aaccgcgcga caaatcgccc gtaccgcccg caatgtccaa cactttatcg ccttttttca
      601 ggtgcgccgt atggatggta aaatgcttcc atacgcggtg cagccctgcc gacatcacat
      661 cattcataat gtcgtagttt ttggcgacgg agtgaaacac ttccgccact tttccggctt
      721 tctcatcttc gttgaccgtg ctgaacccga aatgcgtttt ttgtccgccc atattctaac
      781 cacctccctg ataccgccct acggcatccc aaacttaaca atctgattgc ttttcaaaac
      841 gcgacgcgcc cgccgccgcc aatttgtcca aatattcctg ccacatcgca tcgtatccgt
      901 atgccagtct gtgcagatac gcccaatcgt aaagaccgct gtcgtgcccg tccgaaaaac
      961 tgattttcag cgcatactgt ccgacaggct gcaaatccgt aatctgcaca tccgccttac
     1021 cggtctgcaa aacatcctgt cccacgccgt gtccgcgcac ttccgcactc ggcgaataga
     1081 cgcgtaaaaa ttcggcaggc agatttttag gttcgtcccc ataagtcaaa accaacacgg
     1141 cacggttttt caacaaacgg atttcaacag gaatattatt caaaaaacac ctcggttcgg
     1201 catacaatat ccggcaggcg tacgcgtgcc tttaaacggc gcgtatttta acagctttat
     1261 cccgataccg atattcaaac ccacacttca gacggcattg atatggacaa tctcgtatgg
     1321 gacggcattc ccgacatccg cacactcgac caaaccatcc gcaaacacgc acacccgctc
     1381 aacctgattg tctgcctccc cgataatcag attcccgatt ttcaaaccgc acaagatgct
     1441 tcggactcgg aatgccgtct gaagcaccgt ttggatcagg caacccagtg cctccagttc
     1501 gacagcatca acctcatcga acacatcctg cccgatgtcc gcttctggct ggttccccct
     1561 tcacgcaccc gccgcctgca cgaacacttc caccacattt cctggcagac cgaagccatc
     1621 ccgcaaaccg aaagcaagtc cgacaaaccc tggtttgcac ttccacaaac atccgaacgg
     1681 aaaaaaccgg aacacgtcct cgtcatcggt gcaggcattg ccggcgcatc gaccgcccac
     1741 gccttagcat cacacggcat ttccgttacc gtattggaag cccgaaaagc cgctcaagcc
     1801 gccagcggca accggcaagg gctgctttac gccaaaatct cgccgcacga caccggacag
     1861 accgaactgc tgcttgccgg ctacggctac accaaacgcc tgctcggaca catcctgccc
     1921 gactccgaca cttggggcgg caacggcatc atccacctca attacagccg caccgaacaa
     1981 caacgcaatc acgaattggg tttgcaaaaa caccataacc acctctaccg cagcatcacg
     2041 tctgcagaag ccgaaaaaat cgccggcatc ccgctgaaca cgccctacgc cgaaccatta
     2101 tgcggactct actggcaaca cggcgtatgg ctcaatccgc ccgcattcgt ccgcaccctc
     2161 ctcagccatc cgctgatcga actatatgaa aacacaacgt taaccggcat ttcccacgac
     2221 ggagaaaagt ggattgcaag cacgccaaac ggcacattta ccgccacaca catcatctac
     2281 tgcaccggcg cgcacagccc ctgcctgccc gaaaccaacc tcgccgccct acccctcagg
     2341 caaatacgcg gacaaaccgg cctcacaccg tccaccccgt tttccgaaca actgcgttgc
     2401 gccgtttcag gcgaaagcta catcagcccg tcgtggcacg gactgcactg ctacggcgcg
     2461 agttttattc ccaacagcag caataccgga tggaacgaag ccgaagaagc ctcaaaccgc
     2521 caagcattgg cacaccttaa ccccgccctt gccgaatcat tgtttgccgc caacccaaac
     2581 ccccaaaaac accaagggca cgccgccata cgctgcgaca gccccgacca cctccccgtc
     2641 gtcggcgcac tcggcgacat tgccgccatg cggcagactt acgccaaact tgcactggac
     2701 aaaaactacc gcatcgacac cacatgccca tatctgccca atgcctacac caataccgcc
     2761 cacggcacac gcgggcttgc caccgccccc atctgcgccg ccgccgttgc cgccgaaatc
     2821 ctaggcttgc cccatctctt ttcacaacgc ctgcgccacg ccctacaccc caaccgcacc
     2881 gtcatccgcg ccatcgtcag aaggcagaac ctgattcctt aagcctttgc cgcatgccgt
     2941 cccgaccaca aaaaatcata ttcaaataaa cataccaatc ctcacggtaa aatatcgaaa
     3001 acacccataa cgttttcttc ccaaaataag gagtaaggaa tgcctaacaa attgcctttg
     3061 ctgtccaaga tgaattttcc gtaacattcg aagacttcct taaccctgcc agccgcttcg
     3121 gtttctgtac cccctgttag ataaggaagg gatgaaaaag ccctgttact tgtatgaagc
     3181 aaagggcgtt aatttttgtt aatcttccct tctcaggaac tcaatatata aggatgttaa
     3241 tttttatttc ccttctcgag gactcaatag
//
LOCUS       DAXFZR010000133         3189 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000133 DAXFZR010000000
VERSION     DAXFZR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.134"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            410..628
                     /locus_tag="ACFJAS_002075"
     CDS             410..628
                     /locus_tag="ACFJAS_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983229.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(645..1004)
                     /locus_tag="ACFJAS_002076"
     CDS             complement(645..1004)
                     /locus_tag="ACFJAS_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983230.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(1005..1544)
                     /locus_tag="ACFJAS_002077"
     CDS             complement(1005..1544)
                     /locus_tag="ACFJAS_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFC3983231.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            complement(1704..2408)
                     /locus_tag="ACFJAS_002078"
     CDS             complement(1704..2408)
                     /locus_tag="ACFJAS_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3983232.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWSSNDPLPDDDYVF
                     VPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIA
                     EDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDETAPL
                     DSLSVIGRVFWWSVLD"
     gene            2536..2751
                     /locus_tag="ACFJAS_002079"
     CDS             2536..2751
                     /locus_tag="ACFJAS_002079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983233.1"
                     /translation="MTHSEFIKTLSEQRGAKTEYAKKLGLSLSFLWQIENGRAVTPKR
                     LYKDVMLLTKNKVSISELISEFRVCPK"
     gene            2831..2986
                     /locus_tag="ACFJAS_002080"
     CDS             2831..2986
                     /locus_tag="ACFJAS_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983234.1"
                     /translation="MKKQDRNRLSKKDRRLIKKAMLKAAAKGSDEVYRIAPGLKDGFE
                     LLGKQPD"
     gene            complement(2963..>3189)
                     /locus_tag="ACFJAS_002081"
     CDS             complement(2963..>3189)
                     /locus_tag="ACFJAS_002081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983235.1"
                     /translation="TGFPHNGRRSKKMPSDLSFILTKELIRSGSIRLSGSTAKGQAGE
                     LAVFIRTLHQKPEESEPNTDNEYLIGLLSK"
BASE COUNT          787 a          689 c          829 g          884 t
ORIGIN      
        1 gtttagtgat tatttaataa atactgaaca ttttgattat taaaagaatt tatttttgag
       61 atttcgcagg cgcaaaaaaa accgcctatt aaggcggttg tttaggaaag aatgccctct
      121 gcgcgtgtag ggtatttcga tacttgcgtt tattatatca tatgatataa taaactcata
      181 gattaggaaa tgaagaaacc ccgtatcagc cgatacgggg caacctgaaa gagaaaggag
      241 gcaatgtgat taagaaaatc ctaatctgtg tgattttgta tttccttgca agctccccag
      301 cgtggtaggg aaatacaaaa caagtgatta acgattaacc atttgcaggg cagttaagga
      361 ggctgccctt cctcttatct ttcccatact ctactataag gatttaaaaa tggcaaattc
      421 caacagcggg cattctaaaa aattgcgcgc ggcaacggcg gcggcggcga caaaggcaaa
      481 actggcaagc ggcgaatacc ggcagttttc agtgcagggg cgtgccgaag acgtggagct
      541 tatccttgcc gccgtggaaa aagccggggg cagccgcgta caggctttgg caaagatttg
      601 caggcggtat ctcgaggggc tgtcttaagg ggggtaagac aaggttattt gggaatatcg
      661 ggcaggataa aaggcgggtg tctgtcaaaa ccgatgattt tgataaagcc gtcgcaaagg
      721 gtatagtgaa ggatataggc ggatgtcaaa tcgccatacc tgccgccgtt gtatttggga
      781 atgccgatgt gatagtgcca aaggttgtgc cgttgggcaa atcggacttt ttccagccat
      841 tgaggatcgt ctgccggtac gttgtcgctg gatttgttcc tgccgggtaa ccctgataag
      901 ccgttctgtt ggacgtgtgc gataaattcc gcaatttttc tgcggtcttc tttggggtag
      961 tttttcaacg ctcttttgaa gtcttcgcca agcagcacct gcatttactg ctccaaccat
     1021 ttttccaagt cgtcggcgcc gttgaaatgc gggacgctga caaagctgtc ttcaacggct
     1081 gttttgagtt tttcaagatt gaaattgtaa tccgtctgcg gcagcgcatg gcggaaatat
     1141 tcccccatca gcgcaatccc tatgacttcg ccctgccggg tctgtatcca cggcgcttcc
     1201 tgatgggttt tattgcgtaa tgcccaagcg gtgtagcagc cctgctcttt acggacacgg
     1261 ttgaggacaa ccaactcttc gtccgcatat ttgtccggtt cgatatgggc cgcaggcaat
     1321 gggctgccgc cgtatttttt gtaggtgcgg taaatgcagg ggactaccgg accgtgctgc
     1381 cagtgttcga tattttcggc aaacaggggg cggttaagta tggcaagggc gtagccttgt
     1441 gcgtaataca ggagtttttg aagtttgaga ttggagattt gctccccgtc ctcttcttca
     1501 aaaggggaaa ggaagaaatc tgccacgtcg tacgcgttca gcattttctg tttccgttgt
     1561 tatttttctg tatattaagg ccgccggccc attatttcaa gaaataattt gtgcaaatta
     1621 tccggtatcg gcaggctgtc cgcgccgtcg gcatatcggg cggatatatt tgaaaaatac
     1681 ttgccctgcc gtttcagacg gcatcaatcc aacacgctcc accaaaatac cctgccgata
     1741 acggacaggc tgtccaaagg ggcggtttcg tcgggataga agccgctgtt gtggctgcgt
     1801 atcagcacgc tgttgccggg ctgccgtatc aggtatttga cgcggaacat gccgtcttgg
     1861 gcaaaggcgt atattttgcc gtcgcgtatg gcggtttcgc ccgtatctac ggcgattgcc
     1921 gcgtcttctg cgattttttc ctccatgctg tcgccggtca gggtgcagca gaagacgttg
     1981 tcggggttga tgcctttgcg ccttaacgtt gatttgccga acggcaggcg gtagccgttg
     2041 tagtcgggga tttcatacgc gcccgccccg cctttgaagc agctctcttt gaggtagggg
     2101 acgaaaacat aatcatcgtc gggcagcggg tcgttgctgc tccacatcat cgggcggtgg
     2161 atgtctttga cttcgtgggg caggtcgggg ttgatgagga cgggcgttgg ttcgctcgga
     2221 acggaatcca agtacagact gcccatccca tattcttctt caagccgtct gcttaccttt
     2281 tcgccaaaag atgctttacc gctcatcaat tgagataaaa ggcttttgtc tttaaccggc
     2341 aactttttat tttcaaagaa tctttttaaa ttatcgatac ggatggcctt cagatctcta
     2401 ttagtcattt tcgcgctcca ttcagattgg gattcaattt tatttagttt ctgctaaaca
     2461 aacaaatatt aaattttctt gcctatgcgt ttaatatttg ctaaactcat taaatattta
     2521 cggtgaaact acagaatgac tcattcagaa tttattaaaa cgctgtcaga acagcgtggt
     2581 gcaaaaacgg aatacgctaa aaaacttggc ttatccctgt cttttctatg gcagatagaa
     2641 aacggacggg cagtaacgcc aaagcgtctt tataaagacg ttatgttgtt gacaaaaaat
     2701 aaggtttcta tttctgaatt aatttcggag ttcagggttt gtcccaaata aaaagcccgt
     2761 cgggtcagat gttaattgat ataaccgaat tgaagcggaa gtcatccgca atttaccgga
     2821 aaggaaaaaa atgaagaagc aggacagaaa ccgcctgtcg aagaaagaca gacggctgat
     2881 taaaaaggcg atgctgaaag ccgccgccaa aggcagcgat gaggtttaca gaatcgcgcc
     2941 ggggttgaaa gacggctttg aattacttgg aaagcagccc gattaaatat tcgttatcgg
     3001 tatttggctc cgattcttcg ggtttttgat gaagtgttcg gatgaatacc gccaattctc
     3061 cggcttgtcc tttggccgta ctgccgctta agcggataga accgctgcgg ataagctctt
     3121 tggtaagtat gaaggataag tcggacggca tttttttact ccgtcggccg ttgtgtggaa
     3181 acccggttg
//
LOCUS       DAXFZR010000134         3056 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000134 DAXFZR010000000
VERSION     DAXFZR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.135"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            43..270
                     /locus_tag="ACFJAS_002082"
     CDS             43..270
                     /locus_tag="ACFJAS_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3983236.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPKYKVGNCKNPVAHYSIAKSGIEP"
     gene            949..1452
                     /locus_tag="ACFJAS_002083"
     CDS             949..1452
                     /locus_tag="ACFJAS_002083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983237.1"
                     /translation="MNADAFVSADAETCGRETGEPTSPKAESDSNGNGGLSGKPKNAN
                     VPRRRKTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRWCEMLGTAAPNGKV
                     RFGDKETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGNFVKILEWHPPKTN
                     QAARGRA"
     gene            1449..2810
                     /locus_tag="ACFJAS_002084"
     CDS             1449..2810
                     /locus_tag="ACFJAS_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFC3983238.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAAGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESHYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
     gene            2827..>3056
                     /locus_tag="ACFJAS_002085"
     CDS             2827..>3056
                     /locus_tag="ACFJAS_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983239.1"
                     /translation="MRETCFFCKYADFKTQPDTPVRGFAKCAKARNAEERAKHYPRTN
                     PCAAGAFQTASGAAVAKRTAALGEYPGIPEIE"
BASE COUNT          839 a          723 c          929 g          565 t
ORIGIN      
        1 tgaattgcag ggaagcggtt caggtaacgg cgaaaggcga ttatgaatca aaaacaaacg
       61 caatgcaaac aaattgtcga ttacatccgt aacaagggat gcatcacatc ccttgaggct
      121 tatcagaacc tgaaggtgac gcagcttgcg gcacggataa ccgacttgga aggcaggggc
      181 ttcgtgtttg ccaagccgaa atacaaggtc ggtaactgta aaaatcccgt tgcccattac
      241 tcaatcgcaa agtcaggaat tgaaccatga gccgggaaca aagaatacgc ggagcccgct
      301 tgccggtgcc ggctcatatg caggcggaag acgcgggcaa ggcacgggaa gcgttggacg
      361 ggcgggtcga agaagtgaaa ggggttgatg atgaagccgt ctgaaagttt gagagcggca
      421 ggcaggccaa tcgcgtatta cccgaaattg gcaaagcctt tgggcggtgt aaatgcggca
      481 atattattcg ggcatttctt ctactggaac gataagacgc agtatgaatc aggcatttac
      541 cgaacagcgg aagaaattga aattgaaacc gggctgtccg ttcaggaaca aagaacggca
      601 cgggcaaagc tgagggaacg cggcgtattg attgagactg aaaaacgaat tgaacaccgc
      661 atttactaca aactgaattt agacgctttt gatgatttga tgttgcaaca ttcggggggt
      721 ggggaatcaa cagccccgaa atgcaatatc aacagccccg aacttcaaaa tcaacattcg
      781 gggggtgggg aatcaacagc cccgaaatgc aatatcaaca gccccgaact tcaaaatcaa
      841 cattcgggga gtgaggaatc aacagccgtt ataagaacag aagatttaac agaagattta
      901 gcagtatata cccccttacc cccaaacgcc ggaaacggca aaggcggttt gaacgctgac
      961 gcgtttgttt ccgctgacgc ggaaacgtgc gggcgggaaa ccggcgaacc gacttcgccg
     1021 aaggccgaaa gcgacagtaa cggcaacggc ggcctttcgg gaaaaccgaa aaatgcgaat
     1081 gttccgcgcc gccgcaaaac ccacggcgta ccgcttcagg aaatcgccga tttgtacaac
     1141 gaagttttgg gcggccggtt gccaagcgtc caagtgctga acgacacgcg caaacgggcg
     1201 attgcaaacc gctggtgcga gatgctggga acggcggcgc caaacggcaa ggtgaggttc
     1261 ggggacaagg aaaccggttt ggcctggttt gccggtttct tccggaaagt ggcgatgaac
     1321 ccgttttgga tgggcgaaaa ccaaacaggg tttgcggtcg gcttcgattg gattttcaag
     1381 gcgggcaatt tcgtcaaaat ccttgaatgg catccgccta aaacgaacca ggcggcaagg
     1441 ggaagggcat gaaccgaatc gaggaaacgg aagccgtcca atcactggcc agcgtagggg
     1501 cggaacagaa cattttgggc ggcatcttga ttgaaccgac ggcgattgcg cggtgcgcaa
     1561 tcctgacccc tgaaaagttt taccaggcgc aacacaggat tattttccgc gctctgctgg
     1621 atatggcggc ggcaaacgag cctatcgaca tcatcacgct gaacgacaag ctggaagcgc
     1681 gcggcgaggc ggaaaacgcg ggtggcctgg cttacttgat agacctgaac caaaacaccc
     1741 caagcgcgaa gaatatcagc cggtacgttg ggattgtgaa cgacaggttt gtcgagcgcg
     1801 gcttgctgaa ggcttcggcg gcgattgaaa aaatcgcggt ttccaaagac ggcgggacgg
     1861 tcgcagaaaa gctgtctaag gcggccgacg aattggcggc agccggcaaa gacgcggtaa
     1921 agcgtgaaac caagacattc ggccagaccg ttgaggattt gattggcggt ttggacaaaa
     1981 ggcttgacgg cgtgcgtttc ggattgccta ccggcttgat gaagcttgac gggatgaccg
     2041 gcggtttgcc ggatggaaac ctgattgtga ttgcggcgcg tccgtctatg ggtaaaacgg
     2101 ttttggcgga aaacattgcg cgattcgcgc tgaagcaggg caaggcagtt catttccaaa
     2161 gctacgaaat gagcgcggta gagctggcaa ggcgcggcat ggcggctgag tgcaatatcc
     2221 ccatgcagaa cctgaaaacc ggcaatctga cgcaaagcga ttacgccaat atgccgattt
     2281 acgtcagcca agcgaaagag tggaagtttg acgtgaactg cgacctgctc aacgttgacg
     2341 agctttgctt tttggctaag gagaaaaaac tcactaccgg cttggatttg ttggttgtcg
     2401 atcaccttca catcatgcca agggcaggga gggacgaggt ggcggagttg gggaatatat
     2461 cgcgccgttt gaaaaacttg gcggcagagc tgaatatccc cgtcgtcttg gttgcccagt
     2521 tgaacagggg aaacacaaag caggcagaca aacgcccgaa catggcagac attcgcggca
     2581 gcggcgcgat tgagcaagac gcaaacatca tcatcatgcc gcaccgcgaa agccactacg
     2641 acggaaacga gaatccgagc attgccgagc tgattatcgc caagaaccgg gacggcgaaa
     2701 cgggaacggt ggtttgcggc tggaaagggc aatttatgaa gttcgaggaa gagcctgatt
     2761 tggcatggca agcccccaaa catgatgaat atgaccctta cagtgtctag tgcgggaggc
     2821 cggtaaatgc gtgaaacctg ctttttctgc aagtacgcgg attttaaaac ccaaccggac
     2881 acgccggtgc gcggttttgc gaaatgcgcg aaggcgcgga atgcggaaga gcgggcgaag
     2941 cattacccgc gaaccaatcc ctgcgccgcc ggggcgtttc agacggcatc gggggcggca
     3001 gtcgcaaaaa ggacggcggc gcttggggaa taccccggca tacccgagat tgaaaa
//
LOCUS       DAXFZR010000135         2845 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000135 DAXFZR010000000
VERSION     DAXFZR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.136"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..>2845
                     /gene="pilC"
                     /locus_tag="ACFJAS_002086"
     CDS             <1..>2845
                     /gene="pilC"
                     /locus_tag="ACFJAS_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC3983240.1"
                     /translation="NQPEVKSNVPYSIKDKDRKRVFDYTSQKSSLGRTNNFISFNNND
                     EVVARKNGTAVFGTATYLPPYGKVSGFDADALKERANAVDWIRTTRPGLAGYAYTDVI
                     CRDAGQCPQLVYETKFAFGQQGLQRNGNKLDIYEDKSRDNSPIYKLKDYPWLGVSFNL
                     GSENTVKNGRSSGRLISSFSENNNNQTIVSTTRDRPISLGDRQREHTAVAYYLNAKLH
                     LLDKKRIKDIAQGKTVDLGTLRPRVETTGRNWLSYWATWKIEDKGNITVRLGLPEVKA
                     GRCINKPNPNPKSALSPALTAPALWFGPVQNGKVQMYSASVSTYPDSSSSRIFLQELK
                     TQTEPGKPGRYSLKSLNDGEIKSRQPSFNGRQTIIRLDDGVHLIKLNGSKDEVAAFVN
                     LNGNNTGKNDTFGIVKEANVNLEADEWKKVLLPWTVRGPDNDNKFKSINQKPEKYSQR
                     YRIRDNNGNRDLGDIVNSPIVAVGGYLATSANDGMVHIFKKTGTDQRGYELKLSYIPG
                     TMPRKDIESNDSTLAKELRAFAEKGYVGDRYGVDGGFVLRQVEDRVFMFGAMGLGGRG
                     AYALDLSKTNGNYPAAAPLFDVKDGDKKGKNRVEVELGYTVGTPQIGKTQNGTYSAFL
                     ASGYAAKQIDDSTNKTALYVYDLKDTLGTPIAKIEVKDGKGGLSSPTLVDKDLDGIVD
                     IAYAGDRGGNMYRFDLSDNDPNKWSVRTIFEGGKPITSAPAVSRLADKRVVIFGTGSD
                     LSEQDVVGTDQQYIYGIFDDDKPTVNVKVTNGTGGGLLEQVLSEENKILFLINNKASG
                     GSADKGWVVKLREGERVTVKPTVVLRTAFVTIRKYKDDGCGAETAILGINTADGGALT
                     PRSARPIVPGDQVAQYSGHKTTSKGKSIPIGCMEKGGKTVCPNGYVYDKPVNVRYLDE
                     TETDGFSTTADGDAGGSGIDPAGRRPGKNNRCFS"
BASE COUNT          805 a          726 c          743 g          571 t
ORIGIN      
        1 aaccagcccg aggtaaagtc gaatgtgcca tattcaataa aggacaaaga caggaagcgg
       61 gtatttgact atacgagcca gaaaagcagc ttaggcagga caaacaattt tatctcattc
      121 aacaataacg atgaagttgt tgctcgaaaa aacggtactg ccgtttttgg cacagccacc
      181 tacctgccgc cctacggcaa ggtttccggt tttgatgccg acgctctgaa agagcgcgcc
      241 aatgccgttg attggattcg taccacccgg cccgggctgg caggctacgc ctacaccgac
      301 gtcatatgca gagacgcagg gcaatgcccc caacttgtct atgagaccaa atttgccttc
      361 ggtcaacaag ggttgcaaag aaatggcaac aagctggata tatacgaaga caaaagccgc
      421 gacaattcgc ccatttacaa attgaaggat tatccatggt tgggcgtatc tttcaatttg
      481 ggcagcgaga ataccgtcaa aaatggcaga tcatccggca gattgatatc ttcttttagt
      541 gaaaacaata ataatcaaac catcgtctct acgacacgag accgccctat ttcccttggc
      601 gaccggcagc gcgaacatac cgccgtggcc tattatctga acgccaagct gcacctgctg
      661 gacaaaaaaa ggattaaaga tatcgcccaa ggcaaaacag tggatttggg caccttgaga
      721 ccgcgcgtcg agacgacagg acgaaactgg ctaagttatt gggctacgtg gaagattgaa
      781 gataaaggga acattacagt ccgcctcggc ctgccggaag tcaaagcagg ccgctgcatc
      841 aacaaaccga accccaatcc caaatcagcc ctttcgccgg cactgaccgc ccccgcgctg
      901 tggttcggcc ctgtgcaaaa tggcaaggtg cagatgtatt ccgcttcggt ttctacctac
      961 cccgacagtt cgagcagccg catcttcctc caagagctga aaactcaaac cgaacccggc
     1021 aaacccggcc gctattccct caaatctttg aatgatggtg agattaaaag tcgacagccg
     1081 agtttcaacg ggcggcaaac aatcatccga ttggatgacg gcgtacattt gatcaaactg
     1141 aatggaagca aggatgaggt cgccgctttt gtcaatttaa atggaaacaa caccggcaaa
     1201 aacgacactt tcggcattgt taaggaagcg aacgtcaatc ttgaagctga cgagtggaaa
     1261 aaagtgctgc tgccttggac ggttcggggt cccgataatg acaataaatt taaatcaatt
     1321 aaccaaaaac cagaaaaata cagccaaaga taccgcatcc gcgacaacaa cggcaatcgc
     1381 gatttgggcg acatcgtcaa cagcccgatt gtcgcggtcg gcgggtatct ggcaacttct
     1441 gccaacgacg ggatggtgca tatcttcaaa aaaaccggca cagaccaacg aggctacgaa
     1501 ctgaagctca gctacatccc gggtacgatg ccgcgcaagg atattgaaag caacgattct
     1561 acactcgcca aagagctgcg cgcctttgcc gaaaaaggct atgtgggcga ccgctacggc
     1621 gtggacggcg gctttgtctt gcgtcaagtt gaagaccgcg tgtttatgtt cggcgcgatg
     1681 ggtttgggcg gcaggggcgc gtatgccttg gatttaagca aaaccaacgg caattatcca
     1741 gccgccgctc ccctgtttga tgtcaaagat ggcgataaga aaggcaaaaa tcgcgtggaa
     1801 gtggaattag gctacaccgt cggcacgccg caaatcggca aaacccaaaa cggcacatac
     1861 tccgccttcc tcgcttccgg ttatgcggct aaacaaattg acgactcaac aaataaaacc
     1921 gcgctgtatg tgtatgattt gaaagacacc ttaggtacgc cgattgcaaa aatcgaagtg
     1981 aaggacggca agggcgggct ttcgtccccc acgctggtgg ataaagattt ggacggcatt
     2041 gtcgatatcg cctatgccgg cgaccggggc gggaatatgt accgctttga tttgagcgac
     2101 aacgacccga acaaatggtc tgtacgcact attttcgaag gcgggaagcc gattacctcc
     2161 gcgcccgccg tttcccgact ggcagacaaa cgcgtggtca tcttcggtac gggcagcgat
     2221 ttgagtgaac aggatgtagt cggtacggat caacaatata tttacggtat ctttgacgac
     2281 gataagccga cggttaatgt aaaggtaaca aacggcacgg gaggcgggct gctcgagcaa
     2341 gtgcttagcg aggaaaataa aatcttgttc ctgataaata ataaggcatc cggcggatcg
     2401 gccgataaag gctgggtagt gaaattgagg gaaggagaac gcgttaccgt caaaccgacc
     2461 gtggtattgc gtacagcctt cgtaaccatc cgcaaatata aagacgacgg ctgcggcgcg
     2521 gaaaccgcca ttttgggcat caataccgcc gacggcggcg cattgactcc gagaagcgcg
     2581 cgcccgattg tgccgggtga ccaggttgcg caatattccg gccataagac aacctccaaa
     2641 ggcaaatcca tccctatagg ttgtatggaa aaaggcggta aaaccgtctg cccgaacgga
     2701 tatgtttacg acaaaccggt taatgtgcgt tatctggatg aaacggaaac agacggattt
     2761 tcaacgacgg cggacggcga tgcgggcggc agcggtatag accccgccgg caggcgtccc
     2821 ggcaaaaaca accgctgctt ctcca
//
LOCUS       DAXFZR010000136         2823 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000136 DAXFZR010000000
VERSION     DAXFZR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.137"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            18..941
                     /locus_tag="ACFJAS_002087"
     CDS             18..941
                     /locus_tag="ACFJAS_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC3983241.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFLG
                     ADNWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLHPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLARGEKFFTTQEAEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDAFYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(1020..1661)
                     /locus_tag="ACFJAS_002088"
     CDS             complement(1020..1661)
                     /locus_tag="ACFJAS_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC3983242.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(1716..2507)
                     /locus_tag="ACFJAS_002089"
     CDS             complement(1716..2507)
                     /locus_tag="ACFJAS_002089"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC3983243.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            2673..2748
                     /locus_tag="ACFJAS_002090"
     tRNA            2673..2748
                     /locus_tag="ACFJAS_002090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2706..2708,aa:Thr,seq:tgt)
BASE COUNT          581 a          637 c          876 g          729 t
ORIGIN      
        1 cggttacaat ccgccgcatg aaaaaacgca ataatcctct tccgctgttg aacggtgtca
       61 aacccagtta tttggtgctg ccgcatgaaa agcagtttta cgggctgccg ctgctgcatt
      121 ttctgtgcat ccgctttcct tttttgggag cggacaattg gcgcaggcgg ttgaacagcg
      181 gttttgtggt cggttcggat ggtgcggcgt tggatgaaca ttctttgttc gagccgggta
      241 agacgatgtt ttattatcgg gaaaccagca gtgaggatga gccgcgtatt ccgtttgaag
      301 aaaagatttt gcatattgat gagcatttga ttgtggtgga caagccgcat tttctgcccg
      361 tcatccccgg cgggcgtttt ttgcgggaaa cgctgctcac acgtctgcgc ctgcaccctg
      421 aattgcagca tttgaatgtt gaagacatta cgccgattca ccgcttggac aaggatacgg
      481 cgggtgtgat gctgctgtcg cacaatcctg ccacgcgcgg ggcttatcag acgatgtttc
      541 aaaacaaaac ggtatggaaa acgtatgagg cgcttgcgcc gacaaggacg gatttgccgt
      601 atccgctcga tgtggtttcg cgtttggcga ggggggagaa attttttacg acgcaagagg
      661 ccgaaggcgt gccgaatgcg cacacgacgg tcgaactgat tgaaaacagg ggggaattca
      721 gcctttaccg cctcacgccg cataccggca agaaacacca gttacgcgtg catatgatgg
      781 gtttgggtat gccgctgttg aatgacgcgt tctatcccgt gccgtctgaa gcgggtagcg
      841 aggattatcg gaaacctttg aagcttttgg caaaaaagat tgcgtttgca gatcctttgt
      901 cgggtcggga aagggtgttt tgcagcgggt tttgcctgta atgagaggga acgcaaaccc
      961 gtatcggcag atgccgccgg atacgggttt gatattatcg atgctgtctg aaaggggctt
     1021 tatccgttca ggcatacggg tgcgaagcgg aggatggtgt cgcgcaggac gatgagtttg
     1081 ccgtgaaaga agtgcgtcga accggcgatg gtaatgacgg gcaaatcttg cggttcggcc
     1141 catttcagtg ctttttcgat ttcgacgacc tcatcttccg cgccgtgtat catcagcgtt
     1201 ttggcaacgt tgggaacggc ggacggctcg gggcggtcgg tatagtggca gactgccgca
     1261 ccgatgagca gcaataaatc gggtatgcgc acttgtgcgg caaatgtggc gacatagccg
     1321 ccgaaggaaa agccggataa ggcaaattcc ggggcttcgg gatgttgggc gcgggcatag
     1381 tcgatgacgg cgaggcagtc ttgcgtttcg ccgcgtccgt aatcatgtgt gccttcgctg
     1441 ccgcctacgc cgcgaaggtt gggaaggtag cagtggaagc cgagcttgct caatgctttg
     1501 gcggcggttt ggatgacttt gttggtgttc gtcccgcctt ggagggggtt ggggtggttg
     1561 atgacggcaa caccgcgtgc cggtacttgt tcggacggga tatggatggt ttctaaaatg
     1621 ccggcagggc cgggtatgtg tatggtttcg ggtttcagca taatggtttc ctcttgattt
     1681 tccgcatatt ccgatgcagc cgcgaagatg gcggcttaaa ggtggaaatg cttgccgttg
     1741 ttggggttgg acgctttcag gccggcgagc atgcggtgga agtccaatcc gtatgcgttg
     1801 ctcagtttgt cggcacgttt tttgagtttg tctatgtttt gctctttggg gctgctttgg
     1861 aaggccatta ccgcgacatt gccgtggctt tcggcaggaa gttccaatac gcgcccttca
     1921 aaaacgctca acaaccgttc gatgaagcgt tgatagcgtt tgtcgccgct ccaccagttg
     1981 gtaacgaata tgccgtctga agagagtgcg ttgcggcagt ctctgaaaaa cggttcttca
     2041 accagcgtat cgatgatttg ttcgccgtcg aatccgtcca ccaagatgat atcggtgttg
     2101 tggcggaaga ctttgatgta ttccgcaccg tctgcttcga taatttcaaa ttttccgccc
     2161 tcgaaaggca actcaaacag gttgcgggca atggcgatga cctgaggatt gatgtccacg
     2221 gcggtttggc gcgtgtcggg caggtaggtg tctgtccagc gtgcaaacga accgccgccc
     2281 aagccgattt gggtgatatg ttgcggcagg cgttccgcaa acagcagcca gcccatcatc
     2341 gcgcggctgt aagagagcac cagctcggac gggcggtcga ggttcatcga gctttgaacg
     2401 gtgtcgctgc ccaagtgaag cgaacggata ttgccttctt cggaaatacc gacttcggga
     2461 aagccggatt ttgcaggacg caggcggcgg tagggatgtc ttgccatcgg tgtgaacggt
     2521 cggtttgaaa ggcggatatt ttatcagaat gccgatgctt gttctgtttc aaattaattt
     2581 tgttttaaat aagatactta gtgtattttt atgagggggt cgaacaaatt tcttgccaaa
     2641 gtgtgaagtt gcctgcataa ttcgccttct ttgccggtat agctcagttg gtagagcacc
     2701 tgacttgtaa tcagggggtc ccgagttcga ctcttggtgc cggcaccaga tttgacagcc
     2761 cgattgtgta aaacagtcgg gctgttttgc atttgcggca cggtcggtgg cagggctgcg
     2821 ggc
//
LOCUS       DAXFZR010000137         2787 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000137 DAXFZR010000000
VERSION     DAXFZR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.138"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            64..276
                     /locus_tag="ACFJAS_002091"
     CDS             64..276
                     /locus_tag="ACFJAS_002091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983244.1"
                     /translation="MTFQGHNNRKKAGGYAEYITGGELRLLQQTACRFKAALETAAWK
                     HYVRAIKESEPVPDAEARRKRKKQAA"
     gene            302..496
                     /locus_tag="ACFJAS_002092"
     CDS             302..496
                     /locus_tag="ACFJAS_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC3983245.1"
                     /translation="MDSEYLDPQQCADILSVKKRTFLERYAPRPDFPARISVSKKRFW
                     WKKEEVEGWLDRQKEKRPVM"
     gene            complement(552..636)
                     /locus_tag="ACFJAS_002093"
     tRNA            complement(552..636)
                     /locus_tag="ACFJAS_002093"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Leu,seq:tag)
     gene            787..1461
                     /locus_tag="ACFJAS_002094"
     CDS             787..1461
                     /locus_tag="ACFJAS_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983246.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1454..2047
                     /gene="ribA"
                     /locus_tag="ACFJAS_002095"
     CDS             1454..2047
                     /gene="ribA"
                     /locus_tag="ACFJAS_002095"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC3983247.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            2215..2397
                     /locus_tag="ACFJAS_002096"
                     /pseudo
     CDS             2215..2397
                     /locus_tag="ACFJAS_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2400..>2787
                     /locus_tag="ACFJAS_002097"
     CDS             2400..>2787
                     /locus_tag="ACFJAS_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3983248.1"
                     /translation="MRNAVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLDQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTDKQDAK
                     LIAQYCRSAQESELVKRQKPTDEQYRL"
BASE COUNT          718 a          645 c          782 g          642 t
ORIGIN      
        1 gcgtcagccg catccgcaaa cgctccgcca cacccggaca agccagagaa cggacgaaaa
       61 cccatgacgt ttcaaggaca caacaaccgt aaaaaagccg gcggctacgc cgaatacatc
      121 acgggcggcg aactgcgcct attgcaacaa accgcctgcc gcttcaaagc cgccctcgaa
      181 acggccgcgt ggaaacacta cgtccgcgcc atcaaagaat ccgagccggt accggatgcc
      241 gaagcgcgcc ggaagcggaa aaaacaggcg gcgtaaatat aattaagttt ggaggtttta
      301 aatggatagc gaatacctag acccgcagca atgcgcagat attttatccg taaaaaagcg
      361 tacattttta gagcggtacg caccgcgtcc ggatttcccc gcgcgaatat ccgtatcgaa
      421 gaaacgtttt tggtggaaaa aagaagaagt cgaaggatgg ctagaccgcc aaaaagaaaa
      481 acgcccggtg atgtgatttg caccacattt gcaccaagtg gtatataaca ttatgaaaga
      541 tataaaaatt ttggtgcgga cggagagact cgaactctca cacctctcgg cgccagaacc
      601 taaatctggt gcgtctacca atttcgccac gtccgcataa gggtggagat tatacagatt
      661 ttgtccgatt gcgcaaggct ttggacgtaa tagttgaggc ttatgctttg cagcggtaaa
      721 atccgctatt cgtccgcctg gcatcggaat cgggcggttt tttgttttta ttgacggaat
      781 ttgggtatgc ctgctgcttt gattaaggat tttctgctga ctcagggttt gaagctgccg
      841 cttgacgagg ttcgggcggc gtatctgacg gcgcagacgg taatggatat ggggatggct
      901 tcgattgacc gttctgtttt gtggtgcaat gatgagggtt ggaaacttgc cgattacctg
      961 ccgtgcgatg atgtccgcga agatgccctg aaacggcttt ttatggcttt ggattcggtg
     1021 ttttcacgct cgacaggtgt gcggagcgcg gcggtttatg ccttgatgcc gtctgaaaac
     1081 gccgccctcc ggctggtgtg cctgtcccaa cagggcgaag gtttggaaaa catatgggag
     1141 caggatggaa atattaccga tgtttcgctt gcctgccgtt cggcgcaaag cggttggatg
     1201 aatgttgcct cggatgtacg ccgttggttg aatttgggtg agctttcggg agaacgcaat
     1261 catgcttcgg cggcgcaaat ttccattccc gtttgcacgg agagcggcgg tgtattgggc
     1321 gtggttcatg tggaatttga atgcgcagag tgtgcggata cggcggcgca ggcggaatgg
     1381 gtggctcttg ccttggcttt gtccgaacct ttgaagcaac ttttgggcat cactgccgca
     1441 gaaggagatg aaaatgtctg aattattaga ccatgtcgct tcctgccgtc tgccgaccga
     1501 atggggcgta tttacgatgc acggttttga agaggcaaac gggcaggaac acgtcgcgct
     1561 gaccgtcggc aattgttcag acggcaatcc ggtgttgacg cgcatccact ccgaatgtct
     1621 gacgggcgac gcgctgttct cgagaaaatg cgactgcgga ccgcaacttg aagcggcaat
     1681 gagggcggta caggcagagg ggcgcggcat catcgtctat ctgcgtcagg aaggacgcgg
     1741 catcgggctg attaacaaaa tccgcgccta tcatctgcaa gaacaaggta tggataccgt
     1801 tgaagccaat ttggcactcg ggctgcccgt cgatgcccgc gatttccgtt tggcgcaatc
     1861 tatctacgaa tatctgggca tccgctcggt caaactgttg accaacaacc ccgaaaaaat
     1921 ccaaaccctg aaagatgcgg ggattaacgt ggtcgaacgc attcccctgc acgtcgggga
     1981 aaatctggaa aacgagcgtt atctccaaac caaagcagac aagctggggc atttgatgtc
     2041 ggaataaggc aaagttgcag ggaacgggca tcctgcgccg tctttcggga aacaggtttc
     2101 catagcttga taaagcaata agttttatca agttgcaggg tgcggatgca aacgcattgc
     2161 gagcgcgggt ttgaggcata cgcgcaaaca tcttaataca acgtattgat atttatgatt
     2221 ttctctatca tcgtccctat ttacaatgtg gaaaaatacc ttcgctgctg cgtggattcc
     2281 gtgcttgccg aaaattttgc cgattatgaa atgattttgg tcgatgacgg ttcgccggac
     2341 ggctgcggga agatttgcga cgaatatgca ggcaaatatc cgcatataag ggtaatccca
     2401 tgcgtaacgc cgtaggattg gacatatcca agctgacatt tgacgcaacg gccattgtcg
     2461 gcaatgccga atattcggca aagtttgaca acgattcaaa aggtttagat cagttttcgg
     2521 accggttgaa aagcttggga tgtcagaatc tgcatatctg catggaggca acgggaaact
     2581 attatgaaga agttgccgac tacttcgcgc agtattacag cgtttacgta gtgaacccgc
     2641 tgaaaataag caagtatgca gaaagcaggt tcaaacgcac caaaacagac aaacaggatg
     2701 caaaactgat agcgcagtat tgccggtcgg cgcaggaaag cgagcttgta aagaggcaga
     2761 agcctacgga cgagcaatac aggcttt
//
LOCUS       DAXFZR010000138         2700 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000138 DAXFZR010000000
VERSION     DAXFZR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.139"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..324)
                     /locus_tag="ACFJAS_002098"
     CDS             complement(<1..324)
                     /locus_tag="ACFJAS_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983249.1"
                     /translation="MEKIINSKVRETNFNQAWRVIRAMQINNISDEQVMIVKMSCYLK
                     KDEIFKNIERYGYQSSDIKKINPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIF"
     gene            complement(312..650)
                     /locus_tag="ACFJAS_002099"
     CDS             complement(312..650)
                     /locus_tag="ACFJAS_002099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983250.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLKRTGAEVYINGRRVYHNEA
                     GMASASLRSLMGEYLEFEESGTKITVEIGSAWHFNETIRNINTHLRGTKLPGSNWRVQ
                     DKGHFHLWKR"
     gene            complement(695..1072)
                     /locus_tag="ACFJAS_002100"
     CDS             complement(695..1072)
                     /locus_tag="ACFJAS_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983251.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            complement(1130..1495)
                     /locus_tag="ACFJAS_002101"
     CDS             complement(1130..1495)
                     /locus_tag="ACFJAS_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983252.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(1477..2016)
                     /locus_tag="ACFJAS_002102"
     CDS             complement(1477..2016)
                     /locus_tag="ACFJAS_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983253.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            complement(2059..>2700)
                     /locus_tag="ACFJAS_002103"
     CDS             complement(2059..>2700)
                     /locus_tag="ACFJAS_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC3983254.1"
                     /translation="GSNTDMLAGTLGLRYGLTGNTDIYGSGSYLWHEERKLDGNGKTR
                     NKRMSDISAGISHTFLKDGKNPALIAFLESTVYEKSRNKASSGKSWLIGATTYKAIDP
                     IVLSLTAAYRINGSKTLSDDVKYKAGNYWMLNPNISFAANDRISLTGGIQWLGKQPDR
                     IDGKKESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQSSSELKLGVQHTF"
BASE COUNT          751 a          598 c          512 g          839 t
ORIGIN      
        1 aaatatttca aacattaaaa aatcattaca tgaccaagcc aaatcataaa tttgattcaa
       61 atgggtatca taaagataaa aataataagg tttgggaaca ggtaaaatat ttaatggaaa
      121 aggaggatta attttcttaa tatctgatga ctgatatcca tatctttcta tattcttaaa
      181 aatttcatct ttcttaagat aacaagacat tttgacaatc ataacctgtt catcagatat
      241 attatttatt tgcatggcgc gtataacacg ccatgcctga ttaaaattag tctcccttac
      301 cttagaattt attatctttt ccaaagatga aaatgcccct tatcttgaac tctccagtta
      361 gaacctggaa gtttcgtacc tcttaaatgt gtattaatat ttcttatagt ttcattaaaa
      421 tgccacgcgc tgccgatttc aacggtaatt ttcgtaccgc tttcttcaaa ttccaggtat
      481 tcccccatta gcgaacgcaa agaagcagac gccatcccgg cttcgttatg ataaacccgc
      541 ctgccgttga tatagacttc cgcccctgtc cgtttcaaat tccaaaaatt ccgtactgaa
      601 atttccatat cccgatattg tgcagaccat gttttttcga aggttttcat aaaatttcct
      661 atacctgtcc aatcggcaca tatcaattgc attattacat ctcaatacga taaatatttc
      721 ttaagtcaaa atgcaagcct gaccgtacct caactgtcaa aattttatta ttttttattg
      781 attttaagac aatttctgaa aaattctctt cgctttctcc cttttgtaga agcacatcag
      841 aaaaaataaa actttcccga ttaaattcat aaatatgttt caaccattca cctcctcttt
      901 ctgtaaggca agattcagtt tcattcttcc ttattgtata aatatttcct tcacaaaatc
      961 tgaaataaat ccataaatcc atcttatcca taattaaaga aaaagtttca cctcgagatt
     1021 ttgtcaacaa ttcgcaaggt tgcgatgttg caatcaaata gccgaaagac attttttacc
     1081 tcatacatgg tcgaaatcag tttctgttag ttcagaatcc attttttcgt caacaactga
     1141 atccgcattt ttgaattaac gttttcatca gctgccgttt atctaaaccg gcaggttcag
     1201 tttcagaata agccttatat gaagactgta agcatttcag aaaaagatca tcagaaggca
     1261 tatctgccga atcaaataca actgttttga ttttggtact tacccaaaac cctttttgct
     1321 ctttttctac tatacggaaa ttcagaatat ttccaaccga atcaaaagca cggtaaacat
     1381 catccatcaa atcctgcggc tctattttct tttccaattc cgacaatcct tgaaatatat
     1441 ccaaagacac atcttcaaat agaaaaaaag gaggagttaa aagcggtttt tccatgatct
     1501 gtccgtagat tttgattccc aagggcgatg acgaccaatt ccctgtccag gcaaagtctt
     1561 gcccgtatta tccgtaactc gacgatgata atggggaaat tttccaatag gatgacctgt
     1621 tctattaccg aaaggggcta tccgcatatt attgccgatt ttaatctcac gtccatattt
     1681 agcaaaggaa acaacctttc ctgcagcacc tacatcacca ggaattgcgc ctaatccgcc
     1741 agcaacatct ctaacagaag ctggtctgcc tgtcgttgca taactaaaac catgctgtgt
     1801 ccacatacca atggcagcac cacccaagat agccaatgga agaaaagccc cttcagtctc
     1861 cttcatctcc ttctgagaaa gctccgccaa ctgcatcggc gcatctgccc gcgtgtggaa
     1921 cacttggtct tcaaatgcct gattgtccaa tccgtttgcc attgcggggg cgatcataga
     1981 cagcatcatt acggctgcgg tgatttgttt tttcataata actcctttgg attacaaggt
     2041 tggaaaatca aaatctgctt aaaatgtatg ctgtacgccc aatttcagtt cggaactgct
     2101 ttgccctgaa acgttgaaac gtgcggatgc gtttaaagcc gcggttttgg tgaaaccgaa
     2161 acctgcgccg aaatgggcgt aggtggatgt gtttcttgcg gattcttttt tgccgtctat
     2221 gcggtcgggc tgtttgccca gccattggat gcctccggtc aggctgattc tgtcgttggc
     2281 ggcaaatgag atgttgggat tcagcatcca gtaattgcct gctttgtatt tgacgtcgtc
     2341 tgaaagggtt ttgctgccgt tgatgcggta ggcggcggtg agggaaagga caatcggatc
     2401 tatggctttg taggtggtgg cgccgatgag ccacgatttt cccgacgagg ctttgttgcg
     2461 cgatttttcg taaaccgtgc tttcgaggaa agcgatgagt gcgggatttt tgccgtcttt
     2521 aaggaaggtg tggctgatgc cggcggatat gtcggacatc cgtttgttgc gggttttgcc
     2581 gttgccgtcg agtttgcgtt cttcgtgcca cagatagctg ccgctgccgt aaatgtcggt
     2641 attgccggtc agtccgtagc gcaaaccgag cgtgccggcg agcatatcgg tattgctgcc
//
LOCUS       DAXFZR010000139         2629 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000139 DAXFZR010000000
VERSION     DAXFZR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.140"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..690
                     /locus_tag="ACFJAS_002104"
     CDS             <1..690
                     /locus_tag="ACFJAS_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC3983255.1"
                     /translation="IIAMGAWLEILSEEKDGNRLARHHKHGEIWKKPTRHEDIAAVFP
                     FGNPIHNNTMIMRRSVIDGGLRYNTGRDWAEDYQFWYDVSKLGRLAYYPEALVKYRLH
                     ANQVSSKHSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQTKAAAYELPEKDLP
                     EEDFERARRFLYRCFKRTDTPPSGAWLDFAADGRMRRLFTLRQYFGILYRLIKNRRQA
                     RSDSAGKEQEI"
     gene            690..1529
                     /locus_tag="ACFJAS_002105"
     CDS             690..1529
                     /locus_tag="ACFJAS_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC3983256.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLERA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWEQALDEGLPYIAVFEDDVLLGEGAEQFLA
                     EDTWLQERFDPDSAFVVRLETMFMHVLTSPSGVADYGGRAFPLLESEHCGTAGYIISR
                     KAMRFFLDRFAVLPPERLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRRLNRKQQWRDSPANTFKHRLIRALTKIGREREKRRKRREQTIGKIIV
                     PFQ"
     gene            1541..2462
                     /locus_tag="ACFJAS_002106"
                     /pseudo
     CDS             1541..2462
                     /locus_tag="ACFJAS_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
     gene            2522..>2629
                     /locus_tag="ACFJAS_002107"
     CDS             2522..>2629
                     /locus_tag="ACFJAS_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase 2 family protein"
                     /protein_id="HFC3983257.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLSAVVGQTWRNLDIL"
BASE COUNT          637 a          694 c          739 g          559 t
ORIGIN      
        1 atcatcgcga tgggcgcgtg gctggaaatt ttgtcggaag aaaaggacgg caaccggctg
       61 gcgcggcacc acaaacacgg cgaaatttgg aaaaagccga cccggcacga agacatcgcc
      121 gccgttttcc ctttcggcaa ccccatacac aacaacacga tgattatgcg gcgcagcgtc
      181 attgacggcg gtttgcgtta caacaccggg cgggattggg cggaagatta ccaattttgg
      241 tacgatgtca gcaaattggg caggctggct tattatcccg aagccttggt caaataccgc
      301 cttcacgcca atcaggtttc atccaaacac agcgtccgcc aacacgaaat cgcgcaaggc
      361 atccaaaaaa ccgccagaaa cgattttttg cagtctatgg gttttaaaac ccggttcgac
      421 agcctagaat accgccaaac aaaagcagcg gcgtatgaac tgccggagaa ggatttgccg
      481 gaagaagatt ttgaacgcgc ccgccggttt ttgtaccgat gcttcaaacg gacggacacg
      541 ccgccctccg gcgcgtggct ggatttcgcg gcagacggca ggatgaggcg gctgtttacc
      601 ttgaggcaat acttcggcat tttgtaccgg ctgattaaaa accgccggca ggcgcggtcg
      661 gattcggcag ggaaagaaca ggagatttaa tgcaaaacca cgttatcagc ttggcttccg
      721 ccgcagagcg cagggcgcac attgccgata ccttcggcag tcgcggcatc ccgttccagt
      781 ttttcgacgc actgatgccg tctgaaaggc tggaacgggc gatggcggaa ctcgtccccg
      841 gcttgtcggc gcacccctat ttgagcggag tggaaaaagc ctgctttatg agccacgccg
      901 tattgtggga acaggcgttg gacgaaggct taccgtatat cgccgtattt gaagatgatg
      961 tcttactcgg cgaaggcgcg gagcagttcc ttgccgaaga tacttggctg caagaacgct
     1021 ttgaccccga ttccgccttt gtcgtccgct tggaaacgat gtttatgcac gtcctgacct
     1081 cgccctccgg cgtggcggac tacggcgggc gcgcctttcc gcttttggaa agcgaacact
     1141 gcgggacggc gggctatatt atttcccgaa aggcgatgcg ttttttcttg gacaggtttg
     1201 ccgttttgcc gcccgaacgc ctgcaccctg tcgatttgat gatgttcggc aaccctgacg
     1261 acagggaagg aatgccggtt tgccagctca atcccgcctt gtgcgcccaa gagctgcatt
     1321 atgccaagtt tcacgaccaa aacagcgcat tgggcagcct gatcgaacac gaccgccgcc
     1381 tgaaccgcaa acagcaatgg cgcgattccc ccgccaacac attcaaacac cgcctgatcc
     1441 gcgccttgac caaaatcggc agggaaaggg aaaaacgccg gaaaaggcgc gaacagacaa
     1501 tcggcaagat tattgtgcct ttccaataaa aggagaaaag atggacatcg tatttgcggc
     1561 agacgacaac tatgccgcct acctttgcgt tgcggcaaaa agcgtggaag cggcccatcc
     1621 cgatacggaa atcaggttcc acgtcctcga tgccggcatc agtgaggaaa accgggcggc
     1681 ggttgccgcc aatttgcggg ggggggggta atatccgctt tatagacgta aaccccgaag
     1741 atttcgccgg cttcccctta aacatcaggc acatttccat tacgacttat gcccgcctga
     1801 aattgggcga atacattgcc gattgcgaca aagtcctgta tctggatacg gacgtattgg
     1861 tcagggacgg cctgaagccc ttatgggata ccgatttggg cggtaactgg gtcggcgcgt
     1921 gcatcgattt gtttgtcgaa aggcaggaag gatacaaaca aaaaatcggt atggcggacg
     1981 gagaatatta tttcaatgcc ggcgtattgc tgatcaacct gaaaaagtgg cggcggcacg
     2041 atattttcaa aatgtcctgc gaatgggtgg aacaatacaa ggacgtgatg caatatcagg
     2101 atcaggacat tttgaacggg ctgtttaaag gcggggtgtg ttatgcgaac agccgtttca
     2161 actttatgcc gaccaattat gcctttatgg cgaacgggtt tgcgtcccgc cataccgacc
     2221 cgctttacct cgaccgtacc aatacggcga tgcccgtcgc cgtcagccat tattgcggct
     2281 cggcaaagcc gtggcacagg gactgcaccg tttggggtgc ggaacgtttc acagagttgg
     2341 ccggcagcct gacgaccgtt cccgaagaat ggcgcggcaa acttgccgtc ccgccgacaa
     2401 agcgtatgct tcaaagatgg cgcaaaaagc tgtctgccag attcttacgc aagatttatt
     2461 gacggggcag gccgtctgaa gccttcagac ggcatcggac gtatcggaaa ggagaaacgg
     2521 attgcagcct ttagtcagcg tattgatttg cgcctacaac gtagaaaaat attttgccca
     2581 atcgctgtcc gccgtagtgg ggcagacttg gcgcaacttg gatattttg
//
LOCUS       DAXFZR010000140         2485 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000140 DAXFZR010000000
VERSION     DAXFZR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.141"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..517
                     /locus_tag="ACFJAS_002108"
     CDS             <1..517
                     /locus_tag="ACFJAS_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC3983258.1"
                     /translation="WIKAEDLKAGSRLLSESGKTQTVRNIVVKPKPLKAYNLTVADWH
                     TYFVKGNQAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKNDRAGKDFTKAGKEAVID
                     LNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVIPKSKGGQGTPKNGQVL
                     CRGCNIKKSNK"
     gene            514..933
                     /locus_tag="ACFJAS_002109"
     CDS             514..933
                     /locus_tag="ACFJAS_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC3983259.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            1181..1453
                     /locus_tag="ACFJAS_002110"
     CDS             1181..1453
                     /locus_tag="ACFJAS_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983260.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            1435..1587
                     /locus_tag="ACFJAS_002111"
     CDS             1435..1587
                     /locus_tag="ACFJAS_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983261.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            1574..2119
                     /locus_tag="ACFJAS_002112"
     CDS             1574..2119
                     /locus_tag="ACFJAS_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC3983262.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLNNEVDNLSDMKRKPFAW"
     gene            complement(2155..>2485)
                     /locus_tag="ACFJAS_002113"
     CDS             complement(2155..>2485)
                     /locus_tag="ACFJAS_002113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983263.1"
                     /translation="LPDKAGLPPYCLSSHLLSCHRLLSPRFYRFCQLRGFGGGNVEGG
                     FDGFGGIGVDPYSSANGNTTDGVCFSAVCRFDQGAAVEGAGVGFLKNSVLNILLQMKE
                     RPSEKQA"
BASE COUNT          816 a          450 c          534 g          685 t
ORIGIN      
        1 atggattaaa gcggaagatt tgaaagcggg aagccggctg ttatccgaaa gcggcaaaac
       61 ccaaaccgtc cgcaacatcg ttgtcaaacc aaaaccgctc aaagcctaca atctgaccgt
      121 tgccgattgg catacctact ttgtcaaggg taatcaggcg gaaacggaag gggtttgggt
      181 tcataatgat tgtccgtatg gcggttcaaa taatttagag aaagcaaaac ttcgcgcaga
      241 acggcttagt aaaaacgatc gggcaggaaa agactttact aaagcaggaa aagaagccgt
      301 tattgatttg aataggattc aaaataatgg tcaagttaag tgcgctaatt gcggtattga
      361 aacaattcca gccaaacaat ctataaaaaa tatttctcct acttcaaatg aaagacaagt
      421 agatcatgtc atccctaaat ctaagggtgg tcaaggtaca cctaaaaacg ggcaggtatt
      481 atgcagaggc tgcaacatta agaagagtaa caaatgaaat cgaaacttac tgtcgtctat
      541 tacgatttgg aaagcaatat tgcagaagaa atactgtcag gaaacataat gcctgatgga
      601 aattttctta ttcaagagat tcctcttttc gcaccgaatt tggctttaaa cgacattgtt
      661 gccatagaac gtgaggataa gatgctgttt ttcgaccact tgataaaagc ttcaggaaat
      721 accacgataa acatcgttgt tttggatcat ttcccaaagg atttattggc agccatagaa
      781 gaacacagtg gtaaaatcag aaaaaatgga gagaattatt tatcggttaa ttttccgccc
      841 aaaaaatata attctgattt aaaaggaatt ttaaatagat atgaggaagc aaatatcctc
      901 agctacaggg aagcttgttt gggcttctct taatcgatct ataagtaaga atggattacg
      961 ttagattgta aacctaacga ttaaaaggtc gtctgaaacc gtgtttaatt ttcagacgac
     1021 ctatttcctt catttgaaac aggatattga aaactaagtt cttcaaaaat cctacacctg
     1081 ctccttccac ggcagcacct tggtcaaaac ggcagacggc tacaaagcca atggacgtgt
     1141 aatagaaaat agtgcggtaa caattattcg ggattgaaga atgatgagtg taaaagaatt
     1201 attcaaagtc atcttagatg aaaataaaga ttttcctatt agaacgattc atcgtacccc
     1261 gatggggata cttcccaaac ctgttgcttt aagtattgtt caatatgaaa atgacccggg
     1321 attttatttg ttttatttgg atgagaccgg tcaggaacaa acggatactt atcatgacac
     1381 attagattcg gcatttgaac aggctgaatt tgaatttgga atcagcaaag aggaatggat
     1441 gcaaagtcct tagacggcaa aatcaagatg ccgctttatt tttcagatgg catttccgtc
     1501 aataaaaaag tcggtattgg agcccaaaac agcctacgtc ttgccgggat agggatgaaa
     1561 tctttaagaa aaaatgatta tagttgaaca tttagaacat tatttgggtg aaatcgaaag
     1621 cggtataaaa tgcttggatc gacgttatca tctcagcgtt tccgtcttcc catcacaacc
     1681 atataaaggg gtaactactt tttctacttt aggcttgaat cgatatgact tgaactataa
     1741 gagtaggttt gaattaattt ttacctgttc tgaagaatgg aataaagaaa atattgcagc
     1801 ttttctatca ggagtagccg aatatttgat tgacaataga caacctattt taagaggtga
     1861 aattattcaa ttacccaggg taattatcga aggtagcaaa atggatgcac tgtatgtttc
     1921 cgccccattt tatttcgatg atgattttca ggtctgttat ggcgaacact acaatatcgt
     1981 tttccctttg cttgtcccct tgtataaaca ggaagcagaa ttggtggaaa agaaaggttg
     2041 gaatgctttt gagcagttct tgctgaataa tgaagttgac aacctttcgg atatgaagcg
     2101 gaaaccgttt gcttggtaag ggctttctta aatttgatta aaggtcgtct gaaattaagc
     2161 ttgtttttca gatggtcttt ccttcatttg aagcaggata ttgagaactg agttcttcaa
     2221 aaatcctaca cctgctcctt ccacggcagc accttggtca aaacggcaga cggctgaaaa
     2281 gcaaacaccg tccgtcgtgt tgccgtttgc ggatgagtac gggtcaaccc caatgccgcc
     2341 gaaaccgtcg aagccgcctt caacattgcc accgccaaag ccgcgaagtt ggcaaaaacg
     2401 gtaaaaccgg ggagataaaa gccgatggca ggaaagtaaa tgtgaggata gacagtacgg
     2461 aggcagacct gctttatccg gcagg
//
LOCUS       DAXFZR010000141         2411 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000141 DAXFZR010000000
VERSION     DAXFZR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.142"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            83..1072
                     /locus_tag="ACFJAS_002114"
     CDS             83..1072
                     /locus_tag="ACFJAS_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3983264.1"
                     /translation="MSARLMGMAFKTGIPRGQRFVLVKLCDCANDEGLCYPSQETLAE
                     DTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYRINVALLEKCYAEAAKRKAA
                     RQAKMWEEPLDYEPSDYEPSDFDAKNHQILSGEPSDFALRTVRFCAKNHQILSDEPSD
                     FDGSLYVEPSVEPSVEPSVEPSVEPSGSNARGARAPAGPHPAKPQTAPPETAPAAKAK
                     KTGRHETELSLLADYGITGQAAEDFLQVRKAKRQPLTETAMRLIAADAEKCGMTALQA
                     AEYAIASGWGSFRADWLQNKTFGGSGNRGGPTHNQTAAVPDAGSYGDMPTTDF"
     gene            1086..1868
                     /locus_tag="ACFJAS_002115"
     CDS             1086..1868
                     /locus_tag="ACFJAS_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC3983265.1"
                     /translation="MALRNASDFLGAYGGGVRVERRQCAEHGGYAAKSVLRGVWTGCP
                     ACRKLEAADEMAAYAETLRRGAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMA
                     RAKAAAAEYAANFADVLQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGD
                     MLRTVKDSFGGGGGEAGAVGVFVKPDLLVLDEFGAGSLSETDGRILFSVVNARYERLM
                     PMLVLTNLTAEAFRENTDARIRDRLRDGGGKLIPFDWESYRA"
     gene            1938..2144
                     /locus_tag="ACFJAS_002116"
     CDS             1938..2144
                     /locus_tag="ACFJAS_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983266.1"
                     /translation="MREGAECGRAGEALPANQSLRRRRVSDGIGGGSRKKDGGAWGIS
                     PRNAPNLSGKAGKTLWEYPSLPEI"
     gene            2183..>2411
                     /locus_tag="ACFJAS_002117"
     CDS             2183..>2411
                     /locus_tag="ACFJAS_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HFC3983267.1"
                     /translation="MTVRNTQTETVRTEAAPQQGGNTNPGYYKNRAFECVGFAQYLNF
                     NLGNAFKYIWRHKEKGGREDLEKALRYLERQR"
BASE COUNT          554 a          653 c          813 g          391 t
ORIGIN      
        1 tgtttgccaa gccgcgcatg aaggcgggcg gccgcgggaa gcctgttacg cattattcga
       61 ttgtcaaaaa cggagcggaa gtatgagtgc gaggctgatg gggatggctt tcaaaacggg
      121 tatcccgagg gggcagcgtt ttgttttggt gaagttgtgc gactgcgcca acgacgaggg
      181 cttgtgttat ccgtcgcaag aaacgctggc ggaagatacg ggcttcgccg aaaccgccgt
      241 acggcagcat atcaagtggc tgaaggataa caatttcatc aagtccgccc ggcggcagag
      301 ggggcgggag aggaagtccg acatctaccg catcaacgtc gccctgcttg aaaaatgcta
      361 tgcggaggcg gcaaaacgga aggcggcgcg gcaggcaaaa atgtgggaag aaccattgga
      421 ttacgaaccg tcggattacg aaccgtcgga ttttgacgct aagaaccatc agattttgag
      481 cggcgaaccg tcagattttg cgctaagaac cgtcagattt tgcgctaaga accatcagat
      541 tttgagcgat gaaccatcag attttgacgg ttccttatat gtagaaccgt cagtagaacc
      601 gtcagtagaa ccgtcagtag aaccgtcagt agaaccgtca ggatcaaatg cgcgcggcgc
      661 gcgcgcccct gccggaccgc accctgcgaa accgcaaacg gcgcctcccg aaaccgcccc
      721 ggcggcgaag gcgaaaaaaa ccggcaggca cgaaaccgag ctttcgctgc ttgccgacta
      781 cggcatcacg gggcaggcgg cggaggactt cctgcaagtc cgcaaggcaa aacggcagcc
      841 gctgacggaa acggcaatgc gcctgattgc cgccgatgcg gagaaatgcg ggatgacggc
      901 gctgcaggcg gcggagtacg ccatcgccag cggctggggc agcttccgcg ccgactggct
      961 gcaaaacaaa actttcggcg ggtccggaaa ccgcggcggc ccgacgcaca accaaaccgc
     1021 cgccgtgccg gatgcgggaa gctacggcga tatgccgacg acggattttt gaggggggtt
     1081 cggatatggc tttgaggaac gcgtctgatt tcttgggggc ttacggcggc ggcgtgcggg
     1141 tcgagcggag gcaatgcgcg gaacacggcg gatacgcggc gaaaagcgtt ttgcgcggcg
     1201 tgtggacggg ctgcccggcc tgccggaagc tggaagcggc ggacgaaatg gcggcatacg
     1261 cggaaacgct gcgccgcggg gcgatgcgcg acgcgctgga aaaacgcatc gggcgttcgg
     1321 gcatcgcccc gcggttcaga aactgccgga ttgaaaacta cgccgtcagc gattcgatcc
     1381 cggggatggc gagggcgaag gcggccgccg ccgagtatgc ggcaaacttc gccgatgtgt
     1441 tgcagacggg gcggagcatg attttttcgg gcaggagggg cacgggcaaa aaccaccttg
     1501 cctgcggcat cgcccgcgaa gtcatcgccg ccggcaaaag cgcgctggtc atcacggtgg
     1561 gcgatatgct gcggacggtc aaggacagtt tcggcggcgg cggcggcgag gcgggggcgg
     1621 tcggggtttt cgtgaagccc gatttgctgg tgctggacga gttcggcgcg ggcagtctgt
     1681 cggaaacgga cgggcggatt ttgttttccg tcgtcaacgc ccgctatgag cggctgatgc
     1741 cgatgctggt gctgaccaac ctgacggcgg aagccttccg cgaaaacacc gacgcgcgga
     1801 tcagggacag gctgcgggac ggcggcggca agctgattcc gttcgactgg gagagctacc
     1861 gtgcgtgaaa cctgcttttt ctgcaagtac gcggatttta aaacccaacc ggacacgccg
     1921 gtgcgcggtt ttgcgaaatg cgcgaaggcg cggaatgcgg aagagcgggc gaagcattac
     1981 ccgcgaacca atccctgcgc cgccggcgcg tttcagacgg catcgggggc ggcagtcgca
     2041 aaaaggacgg cggtgcttgg ggaatatccc cccgcaatgc gccgaatttg agcgggaagg
     2101 cgggtaaaac gctttgggaa tatcccagcc tacccgagat ttaaaaaacg cgttaaaacg
     2161 caaatttgaa aggaaatacg gaatgacggt ccgaaacacg caaaccgaaa ccgtccggac
     2221 ggaagccgcg ccgcaacaag gcggcaatac caacccgggc tattacaaaa accgcgcctt
     2281 cgagtgcgtc gggtttgcgc aatacctcaa cttcaacctc ggcaacgcct tcaaatacat
     2341 ctggcggcac aaggaaaaag gcgggcgcga agacttggaa aaagccctgc ggtacttgga
     2401 acgccaacgc g
//
LOCUS       DAXFZR010000142         2359 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000142 DAXFZR010000000
VERSION     DAXFZR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.143"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(182..937)
                     /locus_tag="ACFJAS_002118"
     CDS             complement(182..937)
                     /locus_tag="ACFJAS_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC3983268.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNISWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKDRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            complement(1115..2293)
                     /gene="nirK"
                     /locus_tag="ACFJAS_002119"
     CDS             complement(1115..2293)
                     /gene="nirK"
                     /locus_tag="ACFJAS_002119"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC3983269.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGNYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
BASE COUNT          491 a          569 c          707 g          592 t
ORIGIN      
        1 gattcccgct ttcgcgggaa tgacgaaaag ttgcgggaat gacggttcgg gcattcctta
       61 aattacccgt gtatcgctgt aaatcttaga gatggcggaa tatagcggat taacaaaaac
      121 cagtacggca aggcgaggca acgccgtact ggtttttgtt aatccactat aattgaaggg
      181 gttatcggct tgcgcagcgg aagcccaagt tgtgcaggac atatttggat tgcaggctgg
      241 tgcggatgcc gtagcggagg aaggcggcat agttggacga gtcgctcgcc ccgacagatg
      301 cgccgctgca aaacatttgc gcgttggcat tgccggaaga aagcaggctg ctgttgaaat
      361 cttccgtcca ttcccaaatc agcccgtgca tatcgtaaac accccagtag ttcgggcggt
      421 ctttgccgac atcgtgcagg cctttccgtc cgccgtcggc ataccaatcg agaatagtgc
      481 ggttgtagcc gggttcgttt gagccgtttt tctgcgtggc ggaagcaagt ccggcaaatt
      541 cccattcgtc gatggtcggc aggcgtttgc cttgtgcggc gcaataggcg ttggcggcaa
      601 accaggaaat attggtaacc ggctgtttca attcgcccgc cttcggcgca tagctgcggc
      661 tgccgttttt catccaatgc ttcaggtaag cgggttctgc ctgtttggaa ccgatcctgc
      721 ctttttgcca ttgggggtgg ctgttgacaa attcggcaaa ctcggcattg gtaacgggat
      781 atttatccag tttgaacggt ttgactttaa tcaggccggt atcttttttc agataaagcg
      841 ggcggtagct gccgccttcg atttgaacca tttcggcagc cgccgcttga gtgccggcga
      901 gtgccgtgcc gaggaaaaat aaccggacgt acttcataaa gcctcctgac aggcggttag
      961 cattgatttt tcagacggca ttttaaacaa aaagccgcga atgccgtctg aaaaatcaat
     1021 cttccgaaag gaaagattgg ttgttaaaaa accactgccg tgcgtaatga agtacggcgg
     1081 cagtggtttg tcccgctaat gacgggtatc cgatttaata aacgcttttt tcggatgcag
     1141 aggctgccgg ggcagaagca gccggtgcgg aagcagcagg ggcagaagcc gcgccgctgc
     1201 cggcgtaagc ggtatcactc aatttttgag tcatgatttc agggttttcc gcaccctcta
     1261 ctttcaattg ccccaacgcg cctttgttga atgcgcggaa gatggagtgg tcgaccaaag
     1321 tgtagttgcc cgggatgtcg actttgaatt cgacgatggc agaaccgccg gcaggcacga
     1381 tggtgctttg tacgttttcg ttaatcagtt tgccgccttc aacataaact ttgtcgaaga
     1441 tttcgccgat gacgtggaag gaagacacca agttcgggcc gccgttaccg acgtacatac
     1501 gcacggtttc gcctgctttg gctttcaggg cgttatcgcc ggcgatagcg cctacgtggc
     1561 cgttgaatac aacgtattca ggctgttcgg caacggcttt gtccatatcg aacggttgca
     1621 ggccttgcgc gccttttttg cctttggtgt agaagtcgcc ttggacgatg tagaactctt
     1681 tatccacttt cggcaggcct tctttaggct cgaccaaaat cagaccgtac ataccgttgg
     1741 cgatgtgcat accgaccggc gcgacggcgc agtggtagat gtacaggccc ggttgcaggg
     1801 ctttgaagct gaatgtggaa gtgcggcccg gggcggtaaa ggtcgcggct gcaccgccgc
     1861 cctgaccggt tgcggcgtgg aagtcgacgt tgtgcggaac ggtagaagaa ggattgttgg
     1921 agaattcgac ttcaaccgta tcgccttcgc gtacgcggat catacggccc ggaacgtcgc
     1981 cgtcaaatgt ccagtagcgg tattccaccc cgtcgtccat tttcatggtt ttttcgacgg
     2041 tttccatttt tacgcgtact ttggcaggat agtcgcggtc gattgcggga ggtacttcgg
     2101 gagcgtgggt ggtcaccgca tcgatgacgg gcagttcgcc tgcaggcgtt tcggcggtag
     2161 cttgtgcggc ggaacttgcg gcttctgcgg aagcggcagg ggtttcggca ggggcttgcg
     2221 cggcaggttc gccgccgcag gcggccaatg cgaataagga agcaatcatt gcggctaatg
     2281 cttggcgttt cataatgttt tccttttgta agaaaagtag ggggattagt taagaacttg
     2341 gtgtgattat gcggttgtc
//
LOCUS       DAXFZR010000143         2256 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000143 DAXFZR010000000
VERSION     DAXFZR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.144"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..245
                     /locus_tag="ACFJAS_002120"
     CDS             <1..245
                     /locus_tag="ACFJAS_002120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983270.1"
                     /translation="FCCPMILNRVKCIYDYFQQGGRMNPTKQSKKSYESKRVLKHVSF
                     NTEKEANLLEFSNNLDFSKWVKEKLKHELELEKLKK"
     gene            744..1586
                     /locus_tag="ACFJAS_002121"
     CDS             744..1586
                     /locus_tag="ACFJAS_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC3983271.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALVGRKRIGYSSAYSMIHQRFNVEAVEGIPADKLPE
                     AVAYVHALTLHTGLAGEVPDREPLPAPQPALPISGNALADIAAMVYYGTRMIELGKDV
                     SAPLKQLGCKQAVTMWTVWHETRSILKRSVAALEVLRGYADKDASGRIAACLEGIYGK
                     AAAR"
     gene            complement(1787..2167)
                     /locus_tag="ACFJAS_002122"
     CDS             complement(1787..2167)
                     /locus_tag="ACFJAS_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC3983272.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKPV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
BASE COUNT          595 a          564 c          530 g          567 t
ORIGIN      
        1 ttttttgttg ccctatgatt ttaaatcgag taaaatgtat ctacgattat tttcaacaag
       61 ggggaagaat gaacccaacc aaacaatcca aaaaaagcta cgaatctaag cgcgttttaa
      121 agcacgtttc atttaacact gaaaaagaag ctaatttatt ggaattttcc aataatttag
      181 acttctccaa atgggtaaaa gaaaagctga aacatgaact tgaattagaa aaactgaaaa
      241 aataatagaa atggcttgca tgaatcgtag atacaaatta taatttcaac acttgctgat
      301 tgcaggtaaa aaagaagccc aaaccaagct gtaacttgat ttgggcaggt ggaaagcaga
      361 gcggcaactc tctttccgcg tctatctacc atgaaataga aaggtcattc taccatgaat
      421 actgtccaag ttttaaactt tcaacaaagc tccgtccgtg cttcccccca aaatattgac
      481 gcaatagcgg aataatagta aagttcggtt cacaggtttg gtcgcctgtt tctttcgtgg
      541 ttattgccgc gcttgcggct tttgttttga taagagttcc ctgaagggtg tatgttaaac
      601 atacaccctt gctgaaaaag ccaacgggcg gactttcggg acatctccgg atgtgctgtt
      661 tccacgagca gtcgaccaac ctgaaacgtc cgcctacctc ttcttggtcg aagagaggct
      721 ttcaaacttt cgtggagctt aaaatgcaaa acaccatttc tgtattttct ttcaaatctc
      781 aaaatgtccg tactcaaatc ttgggggctg aaccttggtt ttgccttggc gatgttgcgg
      841 aaattcttca aattcaaaat gcccgtcagt tgccattaaa agaccagggc attcaaaaat
      901 ccagcgttgc aacaaaaaaa ggcaaccaag aattactttt catcaacgag ccgaatctct
      961 accgagtcat cttccgttca cgcaaagccg aagccgtcaa attccaagac tggatatttg
     1021 aagaggttat ccctcaaatc cgcaaaacag gcggctacca aattactccc aaaaccaccg
     1081 ccgacgaccg taccggattg cgccgggcgg ttgccgcgct tgtcgggcgc aaacgcatag
     1141 gctactcctc cgcgtacagt atgatacacc aacgcttcaa cgtcgaagcc gtcgaaggca
     1201 tccccgccga caaactgccc gaagccgtcg cctacgtcca cgcattgaca ctgcacacgg
     1261 gtttggccgg cgaagtcccc gaccgcgaac cgttgcccgc gccgcaaccc gccctgccca
     1321 taagcggcaa cgctttggcc gacattgccg ctatggttta ttacggcaca cggatgatcg
     1381 aattgggcaa agacgtttcc gcgccgctga agcagctcgg ctgcaaacag gcggttacga
     1441 tgtggacggt ttggcacgaa acccgttcaa tcctgaaaag atccgtcgca gccctcgaag
     1501 tgttgcgggg atatgcggac aaagacgcct ccggccgcat agccgcatgt cttgaaggca
     1561 tttacggcaa ggctgcggca aggtaaacgg aaaatgccgc ctgaaggttc ggacggcatt
     1621 tttattgaag ggatgattgg ggatttgtag gcgtttgcag atacgacaag gggcgcattc
     1681 cgcgccccat cacacgcacg gacggcttgt catcgcctgt cgcccgtgcg cggctgccca
     1741 tatttcggaa cagtcttgca agccccttta agggaacggt tttattctag tacagtttga
     1801 atgccttggc aacggctgtt ttaaccgcct gaaaatcctc ttcactgatt attgggatgc
     1861 aacggtcgcg ccctttgggt ttgtatcggt ctaatcgtgc caatccgact gttgccgtca
     1921 tatcgcattt tgcccgacat tggatgtgcg gcttgtccgg taaggggttt tcactcattt
     1981 tgtggtggca gtccgccaaa gggacaggct ctgtgctgct taagggtacg accgttaccg
     2041 gtttgccgtt gtgcctgttt cgcgctatga cgacgacagg gcgtttcttg accatttccg
     2101 gttcttcata accgcgaaag tcgcacatga taactgaacg ttcccttggt tggaatttta
     2161 aaggcattag ccgctcctga cgatgacagg cggctattat agcagttctt acacaaaaaa
     2221 ccgctttgtg taaggcggtt gcaaaaaaaa gccttc
//
LOCUS       DAXFZR010000144         2135 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000144 DAXFZR010000000
VERSION     DAXFZR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.145"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(52..1020)
                     /gene="ispH"
                     /locus_tag="ACFJAS_002123"
                     /gene_synonym="lytB"
     CDS             complement(52..1020)
                     /gene="ispH"
                     /locus_tag="ACFJAS_002123"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC3983273.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQNCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLAAIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1050..1544)
                     /gene="lspA"
                     /locus_tag="ACFJAS_002124"
     CDS             complement(1050..1544)
                     /gene="lspA"
                     /locus_tag="ACFJAS_002124"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC3983274.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          477 a          612 c          545 g          501 t
ORIGIN      
        1 gtctcggcat ttgtgttgcc caaacatccg atgcctgcgt taacgcctgc gtcaacgttt
       61 gcacaaatcg ggtttggttt cgccctcgcg gcgcagctcc ttgggcagga cgaacacgat
      121 gctttcttcc gcgccccccc cttcgcgcac ggtttcatgc ccccatccgc gtatggctgc
      181 caatacttcc cgcaccaaca cttcgggcgc ggacgcgcct gccgttacgc cgactttgct
      241 tttgccttca aaccacgtgc gttgcaggta ggacgcgttg tccaccatat acgcatcgat
      301 tccgcgcgat gccgccactt cgcgcaggcg gttgctgttg gacgaattgg gcgaaccgac
      361 cacaatcacg atgtcgcact gttccgccag ctctttgacg gcggtttgcc ggttggtcgt
      421 cgcatagcag atgtcttcct tgtgcggatt gcggatattg gggaaacgcg cgttcagcgc
      481 ggcgatgatg tctttggttt catcgaccga gagcgtggtt tggctgacat aggcgagttt
      541 gtcggggttt ctgacttcga gttttgccac atctccgacc gtttcgacca aaagcatttt
      601 gcccggtgca agctgcccca tcgtgccttc gacctcggcg tgccccttat gcccgatcat
      661 gatgatttca cagttttggg catccagtcg ggcgacttcc ttatgcactt tcgtcaccag
      721 cgggcaagtc gcatcaaata cccggaaacc gcgctccgcc gcttcctgct gcaccgcctt
      781 cgatacgccg tgtgccgaat aaaccagtgt cgcgcccggc ggcacttccg ccaagtcttc
      841 gataaacacc gcgccttttt cgcgcaggtt gtccacgacg aatttgttgt ggacgacttc
      901 gtggcgcaca taaaccggcg cgccgaattc ttccaaagca cgttcgacaa tactgattgc
      961 ccgatccaca ccggcgcaga agccgcgcgg attggcaagg atgatggttt ttccgttcat
     1021 aagttttgca ttccgtgttc agacggcatt cacgtttttt tgctatcttt gcgatggacg
     1081 atattgtcaa gcaccgccaa caccgcaccg acgcagataa agctgtcggc aatattaaag
     1141 gcgggataaa accaattttg ccaataaaac aataagaaat cgacgacatg accgtgtatc
     1201 aggcgatcga tgacattgcc cgacgcaccg ccgataatca ttgccgcccc gattttgccg
     1261 agggctgcaa actcgtcgcg caagatggcg cgtaccaaat acgcgctcac cgccaccgcc
     1321 agcaccaaaa aaaagtattt ctgccagccg ccctgatcgg caaggaagct gaatgccgcg
     1381 cccggattgt acaccagcgt cagatcgaaa aacgaaggga tgacgttgac gcgttcccga
     1441 tactgaaacg acgacagcac cgcccacttc gaccactggt ccagcacgat ggcggcaagt
     1501 gccaataccc aatagcgcgt tttacttgaa acagatgaag acatattttt caacagccgg
     1561 ataaaagagt gccattctac ccgaaaaccc ccctttcctg tacccgaaac ggcaaatgcc
     1621 gtaatcttga aacccgtcat tcccgcgcag gcgggaatcc ggacctgtcc gcacggaaac
     1681 ttatcggata aaacggttgc ccaaaccccg cgtcctagat tcccacttcc gtgggaatga
     1741 cggttcggtt gcgtagggcc gggtggtcga aaaggcggat tcgatggatt cgatgaaaac
     1801 ggtagacatg ttggattgat ggaaatggcg ggccgaagca caccctacgg cccgccggat
     1861 ttccgcctgc gcgggaatga cggggtttca ggttgctgtt tcaggtttct gtttttaagt
     1921 ttctgtttcg gtttgatatt ttatagaaat gacgaaattt tagattgcaa gaacttatcc
     1981 cctccgccgt cattcccacg gaagtgggaa tctagaaata aaaagcagca ggaatttatc
     2041 ggaaataact gaaaccgaac ggaccggatt cccgcctgcg cgggaatcta gaatctcgga
     2101 ctttcagata atctttgaat attgctgttg ttcta
//
LOCUS       DAXFZR010000145         2072 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000145 DAXFZR010000000
VERSION     DAXFZR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.146"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            783..1892
                     /gene="fic"
                     /locus_tag="ACFJAS_002125"
     CDS             783..1892
                     /gene="fic"
                     /locus_tag="ACFJAS_002125"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC3983275.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          586 a          525 c          467 g          494 t
ORIGIN      
        1 tgccctctct ctaactctct cccgcaggga gagaggacta tgatgcccac cggcggcttg
       61 ctattaggaa tatctgaatc gtcattccca cgaaagtggg aatctagaac gtcaaatctc
      121 aagaaaccgt tttatccgat aagtttctgt tccgacaggt ccggattccc gcctgcgcgg
      181 gaatgacgaa tccatccata cggaaacctg catcccgtca ttcccacgaa cctacattcc
      241 gtcattcccg cgaaagtggg aatctagttt tttgagtttc agtcattccc gataaattgc
      301 cttagcattg aaggtccgga ttcccgcctg cgcgggaatg acgggatttg aggtttctgt
      361 ttttaatttt ctgttttcac gggaatgacg gtttaaaagt tacccgaaac ctcaaaaaaa
      421 agctgtgttt taatatagtg ttgatagacg tacttggcct ccatgtattc aatcgtggaa
      481 atctatatct tcgtcctcgc cgaaatagtc tatgcccgat atacaatttt gatacacaaa
      541 cttggaaata tgggtatcgt cgccggagcg atagaatgcg gacagtttat atatatacgg
      601 tttttttagg ggagcgggca gatgaagggc ggattttgaa ttaaccccat gcaaattgac
      661 ttttgcgggc ggttttaccg cccatatatt tacaaaagcc aaatttttaa acatatatcc
      721 ttgatatata cacggcgtaa acatatactg gaaacatctt taaattttcc gaaattttaa
      781 acatgagcaa ctggaaaccc gatattccct ataacgattt accacccctg ccgccaaaac
      841 aggatattga aagcaaaacc atcctgaaac gttgtatagc cgcccgtgca tcccttgccc
      901 gtttaaagca ggcggcagaa ttgataccga atcaagccat gctgattaac acccttcctg
      961 ttatggaagc ccgtgcaagt tcggaaattg aaaacatcgt aaccaccacg gacaagctgt
     1021 ttcaatccct gcaaatggat acggaacggc aagaccctgc cacgaaagaa gccctgcaat
     1081 accgcaccgc cctgtttgca ggctatgaat cactggcgag ccgcccttta tgcacacaaa
     1141 ccgccatcat ggtctgcaac gccatcaagc acccctacga aacggccatc cgcaaaacag
     1201 gcggcacagc cctaaaagga ggtaacagcg gaaatgttgt ctataccccg cccgaaggag
     1261 aagaaaccat acgcggcaag ctggcaaatt gggagcggtt tattcacgaa agcggcgatt
     1321 tagacccgct tgtcatcatg gcggcggcac attaccaatt tgaagccatc cacccgttta
     1381 cggacggcaa cgggcggacg gggcgcatat tgaacagcct gctattgatt gaaaaagggc
     1441 ttttggattt gcctattttg tatttgagcc gctacatcat cgaaaacagg gcggactatt
     1501 accgcctgct tttaggcgta accgaacggc aggattggga aagctggata atctacatct
     1561 tagacggcgt agccgacacc gccgattgga cggtatcgaa aatagatgcg atacgctgcc
     1621 tgttcgagca gacacgtcaa cacatacgga cacacgcaca aggaatctac acgcacgaac
     1681 tggtaaatct tctgtttgag cagccatata cacgcattgc caacctagaa gcggcaggga
     1741 tagccaaacg gcagacggcc tctaagtacc tgaaagagct ttcaggcata ggtgtgctgc
     1801 aagaaatcgc catcggcagg gacaaactat tcattcatcc gcgcctaatg gaactattgc
     1861 ggggagaggg caacagcttt acttcattct aaccccctct tcatccctac atgactaaca
     1921 cgaaacaggg attttgacac ccgaaccggt accccttgta ttttccccgc gaaaagccgg
     1981 catccgcccg cgcatcatgg gagcaacaaa acccctgcct aaaattttga cttgtgcaaa
     2041 ttaggggtat atttgggggg tatattgaaa aa
//
LOCUS       DAXFZR010000146         2011 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000146 DAXFZR010000000
VERSION     DAXFZR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.147"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            31..1704
                     /gene="recN"
                     /locus_tag="ACFJAS_002126"
     CDS             31..1704
                     /gene="recN"
                     /locus_tag="ACFJAS_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC3983276.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWADAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDT
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            1815..1891
                     /locus_tag="ACFJAS_002127"
     tRNA            1815..1891
                     /locus_tag="ACFJAS_002127"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1849..1851,aa:Arg,seq:acg)
     gene            1909..1983
                     /locus_tag="ACFJAS_002128"
     tRNA            1909..1983
                     /locus_tag="ACFJAS_002128"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1942..1944,aa:Glu,seq:ttc)
BASE COUNT          534 a          495 c          546 g          436 t
ORIGIN      
        1 tgtcggtaga attgctaagg aatcctcaca atgcttctaa cactttcttt gcgtgatttt
       61 gtcattgtcg aaaatctgaa tctggatttt caaagcggtt ttaccgtttt gaccggcgag
      121 accggcgcgg gcaagtccat tactttggat gcgattggtc tgctgttggg cgataaggcc
      181 gattacagcc aagtccgcag cggcgcaaaa gaagcgcagt tgtcggcatt gttcgatatt
      241 tcccatttgc ctgctttaaa agcagaattg cgtgaacaag ggctgttgga tgagggcggg
      301 gaagaactca gtatccgccg cattatcgat gccaaaggca aaagccgcag ttttatcaac
      361 aatcaggcgg ctaccttggc gcaactcaaa gccgtcggcg ggcagcttat cgacatccac
      421 gggcaaaacg accatcattc gcttaatcag gaagctgccc agcgcgaatt gttggacgca
      481 tttgtgggcg gcagggtgca ggcggaaacc gtcaggcagc tttatcaaaa ctgggccgat
      541 gcgaaaaaag ccctccaaga ggcgcaggaa cacgccgatg ccgtcattat cgagcgggag
      601 cgtctggaat ggcagtttaa cgaactgaat cagttggaca ttaaacaagg cgaatgggaa
      661 gctctcagcc aaagccacga cacccttgcc cattctgccg aattgttgca ggccgccgaa
      721 gaagtcggaa gcaagattga cggcgacaac ggcatccaac gccatatcta tcaatgtcaa
      781 aaactattgg ccaatctgca aaacatcgag ccgcgctttg ccgagagcct gaatatgttg
      841 gcaagcatcg aggccgaatt gggcgaaatc agtgccaata tgcgcgatgt ggcaggacac
      901 agtgacatca accccaatga attggcggca caggaacagc gtatgggtga actgatgggg
      961 atggcgcgga aataccggat tgaacccgaa gagttgcctg ccaagttggc agaaatcgaa
     1021 gaacgcctgc aaagtcttca agctgccgct gatttggacg cgctcgagca taatgttgcc
     1081 cacaattttg ccgaatatca ggaagctgcc cacatccttt ctgccatgcg ccatcaggcg
     1141 gcagggcgtt tgagtagcga aacgaccgag catatgcaac accttgccat gaaaggcgcg
     1201 cgtttcgaca tcgtcctgtt gccttcgtcg ccgacggcac acggtttgga gcaggttcaa
     1261 tttcaagttg ccgctaacaa aggcaatccg ccccgcccgc tgaacaaagt cgcctccggc
     1321 ggcgaattgg cgcgtatcag ccttgcctta caggttgttg ccagccaata tacccaagtt
     1381 cccaccctga tttttgatga ggtcgatacc ggtattggag ggggagtggc tgaaatggtc
     1441 ggcaaggcat tacgtgcgtt gggcagaaaa catcaggtgc ttgccgttac ccatcttccg
     1501 caagtcgcat cctgcggaga aaaccactgg cgggtgcgca agcacagcga gggagagcaa
     1561 accgtcagcg aaatcagtat attggatgaa atacaacgga tcgaagaggt tgcccgtatg
     1621 ttgggcggag aagttattac cgatacgacg cggcaacatg cggcagaatt gctgcaactt
     1681 gcgtcgaaaa atagtttatt ttaaaatcaa tcagttacaa aaataactga aaatagaagt
     1741 ctaaaataat agacaggact cagataaatc cgtattatca cgctttctta atcacttgaa
     1801 caagtgattg tgccgcaccc gtagctcagt tggatagagt atctggctac gaaccagagg
     1861 gtcgggcgtt cgaatcgctc cgggtgcgcc agtaagaaaa tacaatatgc gcccatcgtc
     1921 tagcggttag gacatcgccc tttcacggcg gtaaccgggg ttcgattccc cgtgggcgtg
     1981 ccaattcaaa atgcctccga ttatatcgga g
//
LOCUS       DAXFZR010000147         1959 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000147 DAXFZR010000000
VERSION     DAXFZR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.148"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..698)
                     /gene="mafB"
                     /locus_tag="ACFJAS_002129"
     CDS             complement(<1..698)
                     /gene="mafB"
                     /locus_tag="ACFJAS_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3983277.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDNHDSRSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKINTVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWENNPNQNW
                     WGNRMDDIRGIVQGA"
     gene            complement(738..1700)
                     /gene="mafA"
                     /locus_tag="ACFJAS_002130"
     CDS             complement(738..1700)
                     /gene="mafA"
                     /locus_tag="ACFJAS_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC3983278.1"
                     /translation="MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDMFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRNNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNSHEGYGYSDEAVRQHRQGQP"
     gene            complement(1770..>1959)
                     /locus_tag="ACFJAS_002131"
     CDS             complement(1770..>1959)
                     /locus_tag="ACFJAS_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3983279.1"
                     /translation="RQNHINGIGNFWNRAKRHLRKFDGIPKEHFEPYLKECERRFNNS
                     EIKVQISILKQLVKSSLS"
BASE COUNT          472 a          454 c          458 g          575 t
ORIGIN      
        1 gcaccttgga cgatgccgcg aatatcatcc atacggttac cccaccaatt ttgattgggg
       61 ttgttttccc atatcagttt tcctgcttca tcagcacggc tgaaaaaacc ggaagcggca
      121 ccggcatttt cttttagcca gcggtctgaa aaaggggctt gcggaacagt gtttatcttt
      181 gtttttgtag aagttccttt gacatagtag ctgtatatat cccttgctcc tccggggggc
      241 ggataatcgc tgccttgcgg cccgtcatat ccatcctccg gatggatttc cgaccctgtc
      301 cgatgaagtt ggtaaacggt aaaaccaccg tctacaccgc cgctgaaatc agaagtgctt
      361 cttgaatcat gattatcgaa cggactgtgt acttcgtgtc cgtgccctga aaaatgggtt
      421 tcatagccga taacaccttc aaatcctgtc cgttcgtgtg taataggcag tatggggccg
      481 accgcagttg catcaaatgt ttggacggcg taaacccgat ttttaacact gccgcgacca
      541 ttaccgaaca gatggtattt gccacccggt tcgtagtgtt ttgcctgcat atcatcgcgc
      601 aaacgggcat ccaaaccgtt cgcatgacta atggggattt gcagcaacga tattgccgct
      661 gcaaacagca tcatgaattt ttgaataggc aaattcattt tgttttcctt ggcagcaagc
      721 ggttatggca gtgtgaatca aggttgccct tgtctatgtt gtcgcactgc ttcatcgctg
      781 tatccatacc cctcatgact gttatcagcc tctacggatg gggcggagtt acccgtatga
      841 ttgccgtatg gccggatatc ggagaaatcg accattaatc cttccgtcgg tttgattcct
      901 ttgcttactt tatacggccc catccacaat gcgtaatttt ctttataggc agcttcaaac
      961 gcattggttt tgggtttgat gagcaatttt ttgttatttc tgtctactgc gaaatattcc
     1021 agttttgttt gggctttcag tgtttcggca ttgtataggt gcatttcggt tctgttgcgt
     1081 atcgttccga atacgtcgat gttaataaac atatctgtat cggcattggc aggagaaaca
     1141 acgtctatgc cgcgcaggaa aaatacggtc tgtaccaagt gggaaagaaa ggcagtgtcg
     1201 cgcgggttgg tcgtcaaggt ttcatttcga taatccccca tcccgccaat atttaagccc
     1261 agactgctcc tacttccgct accgtctgat tgggtgcgcg agagtgcagg ggcattaagt
     1321 gtagataaag aagtggttaa acccgtcaaa ccgcctgatg ttgtttcagc ggtggtttcg
     1381 taacgcggat aggtgtaatc ggtacggacg gcagggctgt ttatgtattc gccgcgaatc
     1441 agtgcatcaa tggagtagcg accccctgtc aaactgcctg aaccttggtc gcccatagtt
     1501 gcaatgtaca atgcaacttt tcgtccgtgt aatgcctgta aatccatgtc tttaacggca
     1561 gctctggcag aagcggccac aagttcttgt tcgaccgcga agcgtttgcc tccgccatgc
     1621 gatggaatac ctgtcagtgt cccgcaggcg gataaaataa aaactgaaaa aagaataggt
     1681 atcagcagcc gtgcccgcat agattttctc ctttgatgaa aaacaaattg tatcaaaatt
     1741 gtaaataaat taggggccgt cctggacaac taggataaac tcgattttac taattgtttt
     1801 aaaatggaaa tttgaacttt tatctcactg ttgttaaaac gccgttcgca ctcctttaaa
     1861 tacggctcaa aatgctcttt gggaatgccg tcaaacttgc gtaaatgacg ttttgcccgg
     1921 ttccaaaagt tcccaattcc attaatatgg ttttgtcgt
//
LOCUS       DAXFZR010000148         1947 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000148 DAXFZR010000000
VERSION     DAXFZR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.149"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            50..1843
                     /locus_tag="ACFJAS_002132"
     CDS             50..1843
                     /locus_tag="ACFJAS_002132"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC3983280.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVANGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
BASE COUNT          494 a          532 c          537 g          384 t
ORIGIN      
        1 cctgtccata tttctgctgc gggctgattt attttagaca aggactacca tgcaattaga
       61 tatagaccgt ctggtcgcct atttcggcgg cgtgaacgcg cttgccgaag cgttgaaacg
      121 gcacgatcct gaaaatgccg cgacgaccgc cgccatctac aaatggcgca cacgcggctc
      181 gctgcctttg gcgcaactgc aaaaattgac cgcgcttgcc gaatcgcaag gaagaccgct
      241 ggatttgaat gcttttttac aaaaaaacga atctctggag agaacagaaa tgacacaggc
      301 caaccgcgtt attattttcg acaccaccct gcgcgacggc gaacaatcgc ccggcgccgc
      361 tatgaccaaa gaggaaaaaa tccgcgttgc ccgccagttg gaaaaattgg gcgcggacat
      421 catcgaagcg ggttttgccg ccgccagccc gggcgatttc gaggcggtca atgcgattgc
      481 gaaaaccatt accaaatcaa cggtctgttc attgtcccgc gccatcgagc gggacatccg
      541 tcaggcgggt aaggccgttg cgcccgcgcc gaaaaaacgc atccacacct tcatcgccac
      601 cagccccatc catatggagt acaagctgaa gatgaagccg aagcaggtga ttgaggcggc
      661 ggtcaaagcg gtaaaaatcg ctcgtgaata caccgacgat gtggaatttt cctgcgaaga
      721 cgcgttgcgt tcgcaaatcg atttccttgc cgaaatctgc ggcgcggtga ttgaagcggg
      781 cgcgaccacc atcaatattc ccgataccgt cggctattcc atcccgtaca aaaccgaaga
      841 atttttccgc gaactgattg ccaaaacgcc caacggcggc aaagtcgtct ggtcggcaca
      901 ctgccacaac gatttgggct tggcggttgc caattcgctt gccgcattaa aaggcggcgc
      961 gcgtcaggtg gaatgtaccg ttaacggttt gggcgaacgc gcggggaatg cttcggttga
     1021 agaaatcgtg atggcgttga aggtgcgcca cgacttgttc ggcttggaaa ccggcatcga
     1081 taccacgcaa atcgtgcctt cgtccaaact ggtgtccacc attacgggct atcccgtgca
     1141 gcccaataaa gccattgtcg gcgcgaatgc cttttcgcac gaatcgggca tccatcagga
     1201 cggggtgctg aaacaccgcg aaacttacga gattatgtct gccgaatcgg tcggttggtc
     1261 ggcaaaccga ttgagcttgg gcaaattgtc cggccgcaac gccttcaaaa ccaagctggc
     1321 ggatttgggc atcgagttgg aaagcgaaga ggcactgaac gcggcatttg cacgcttcaa
     1381 agaactcgcc gacaaaaaac gcgaaatctt cgatgaagac ctgcacgcgc tggtgtcgga
     1441 cgaaatgggc aatatgaatg ccgaaagcta taaattcatt tctcaaaaaa tcagcaccga
     1501 aaccggcgaa gagccgcgcg ccgacatcgt gttcggcatc aaaggcgaag aaaaacgcgc
     1561 ttccgcaacc ggctccggcc ctgttgatgc aattttcaaa gcgattgaga gcgtggcgca
     1621 aagcggcgcg actttgcaga tttattccgt caacgccgtc acgcaaggta cggaaagcca
     1681 aggcgaaacc agcgtccgtt tggcgcgcgg caaccgcgtc gccaacggtc agggcgcgga
     1741 taccgacatt ttggcggcga ccgccaaagc ttacctttcc gctttgagca agctggaatt
     1801 tagtgccgcc aaaccgaagg cgcagggcag cggtacgatt tgagcgtgaa aacagacgat
     1861 gccgtctgaa gcataaaaag gcttcagacg gcattgcggc gatatagtgg attaaattta
     1921 aaccggtacg gcgttgcctc gccttgc
//
LOCUS       DAXFZR010000149         1817 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000149 DAXFZR010000000
VERSION     DAXFZR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.150"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(95..1817)
                     /locus_tag="ACFJAS_002133"
                     /pseudo
     CDS             complement(95..1817)
                     /locus_tag="ACFJAS_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
BASE COUNT          438 a          457 c          412 g          510 t
ORIGIN      
        1 ttgtccggta cagccccaaa cataaaacaa aagctgcctg caaaaaaccg gcagcttttg
       61 tgcatcttgt catacattat tcacttcaac cgcatcaaaa cgaataaccc acctgaaacc
      121 ccgttaccca tttcttcgtc tgaaaatatt cgggcttttt caatgcacgg ccggtaaata
      181 tatcgtaatg caggttgccg ccaagcttta tctgcccgcg tatcccaatt gctgtgccgg
      241 ctagagtttg gcccgataac catttggcgg attgtcctga aacatgtcct acatcagccc
      301 caagataaag ctgatggcct ggtttaaatt gccagctcaa atcgttgcgc caataccatc
      361 cccgctcggc aggcaaactc atttcaccgt cgaagccacg tacggtgtgg tgtccgccga
      421 tagccagttt gtcttgcgat gttagcgggg ttttgttcca ttgtgcatga acggatgtgt
      481 cataggcaaa tagctgttta ccgatttgaa aaggagtatt tacatcagcc gatgccgtcc
      541 aaattttcat acgtgacgtg ccttcgccaa aggcttcttc aggcgcgcgc agagcatctt
      601 tcatgccggt gacgtgttta tatttcaact taaaatctgc cgtactgcga ccgatatatc
      661 ctttgtggga aagttctgcc aaccaacctg tggttttacg ccgttgtaca gtcagttcgg
      721 catcatcaat gtaacttttt gtttccctcg tccacagttt tacactgaga taggttttgc
      781 gtttggcatc acgatacaac aggcggttga agccgaaatc agtgttgtaa ctttttccat
      841 tatagtcata gacttccgat aatccggaaa ccgcctgatg gtaacggtag ccattgtgat
      901 tgaatgccca tgtccattta ccgaaagggg ctgaataatg tacggcgtaa ttgtttgatc
      961 cgccttcttt gcgatggccg tcaaaatttt cctcatcggg cgtaccgcca attgaacgtc
     1021 cataatttac atagaacata tcactcagtc caaaaggatt gtcggcagag aaagtgatat
     1081 ttccttggta ttttcctgtc gcctcactac ccgaattatc catccccaca ctcacacagt
     1141 agggcagcag acgttaccgc cattgcacca cgacatcact ttggtttggt tctctctcta
     1201 cgggaacgat ttggagatcg gcttccgcag tcgggagaca tttgagattt tccagtcctt
     1261 gttccaaatc acgcagattc aacagatcgt tcgagcgggt gggaaatttg ttttggaatg
     1321 ctgcaatacg tcctgcatgg gtttgatcat cgttggaccg atcgattcgt atggagcgca
     1381 gatagcccgg catcagggtt aattgaagct tgccactatt caaatcctgt ggcgcagcca
     1441 agatacgggt cgtggtatat cccctgccga tcaaagcatt ttgtgctaag gacatgattt
     1501 gattaatgtc gcccgcatgc agacacttgc cggaaacaaa atgtgtttgg cacaaggcat
     1561 ggttgagcgc aaaccggaat ttagccgctt cttcacccac caattccacc tcagaaatag
     1621 gaaagcaggg gcttgcctca tccgaatttg caccgcctac ctgtgttgcc atcttctcta
     1681 tgcccgtatc ggtaccatcc aaacgaacat cttgttccgg ctgcatctgt tcacgcaatt
     1741 gcttctcacg ttgacgttgg agaatatcct gttgttgtga aacagcttca ggtgtggggg
     1801 cggctgatgt tagggtg
//
LOCUS       DAXFZR010000150         1785 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000150 DAXFZR010000000
VERSION     DAXFZR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.151"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..248
                     /locus_tag="ACFJAS_002134"
     CDS             <1..248
                     /locus_tag="ACFJAS_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983281.1"
                     /translation="KPVIPAQAGIRICRDSLKSGGGGMVLQINDVDTNSGEFAIYTAQ
                     DASVKLDLLKMELKHCKEMLEQKDKEIELLRKLTETV"
     gene            337..906
                     /gene="rsmD"
                     /locus_tag="ACFJAS_002135"
     CDS             337..906
                     /gene="rsmD"
                     /locus_tag="ACFJAS_002135"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC3983282.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRNAKRVVIADNNRQTVQTLEKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1029..1280
                     /locus_tag="ACFJAS_002136"
     CDS             1029..1280
                     /locus_tag="ACFJAS_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC3983283.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1377..1460
                     /locus_tag="ACFJAS_002137"
     tRNA            1377..1460
                     /locus_tag="ACFJAS_002137"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1411..1413,aa:Tyr,seq:gta)
     gene            1491..1564
                     /locus_tag="ACFJAS_002138"
     tRNA            1491..1564
                     /locus_tag="ACFJAS_002138"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1523..1525,aa:Gly,seq:tcc)
     gene            1574..1648
                     /locus_tag="ACFJAS_002139"
     tRNA            1574..1648
                     /locus_tag="ACFJAS_002139"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1606..1608,aa:Thr,seq:ggt)
     gene            1697..>1785
                     /locus_tag="ACFJAS_002140"
     CDS             1697..>1785
                     /locus_tag="ACFJAS_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC3983284.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTA"
BASE COUNT          504 a          399 c          462 g          420 t
ORIGIN      
        1 cgaaacccgt cattcccgcg caggcgggaa tccggatttg tcgggattcg ctcaaatcgg
       61 gcggcggcgg gatggtgttg cagattaacg atgtggatac caacagcggg gaatttgcaa
      121 tctataccgc tcaggatgca tcggtaaagc tggatttgtt aaaaatggaa ttaaaacact
      181 gtaaagaaat gttggaacaa aaagacaaag aaatcgagtt gctccgcaag ctgaccgaaa
      241 ccgtttaaac agatatgccg tctgaaaaaa gttttcaggc ggcatattct ttgacaggtc
      301 ttgtataata ccgtttgaac tttcaggctt ttgattatgg cggcaggcaa acataccaaa
      361 cacagcaacc gggtacgcat tatcggcggg caatgccggg gcaggaaatt gagttttgca
      421 tccgccgacg gactgcgccc aacccccgac agcgtgcgtg aaaagctgtt taactggctg
      481 ggacaagatt tgacgggaaa aacggttttg gatctcttcg gaggcagcgg cgcactcggc
      541 atggaggcgg catcgcgtaa tgcaaaacgg gtcgtgattg cagacaacaa ccgtcaaacg
      601 gtgcaaaccc ttgagaaaaa cagccgcgaa ctgggtttgg ggcaggtgca aaccgtctgt
      661 tcggacggca ttgcctattt ggcaaaccta aaagagaaat tcgatgtcgt cttccttgat
      721 ccgccgtttg cgtggcaaag ttgggaaagt cttttcaatg tattgggcac acgcctgaat
      781 gacggcgcat atgtttatat cgaggcaggc aggcagccgg acaaacctga ttggctgacg
      841 ggatataggg aggggaagtc ggggcagggt acatttgaat taagggtttt ccaagtggct
      901 gaataatatg cgctttgata atcatttccg ggttgtaaac attcgtttgc aaccgtccgg
      961 ttcaaaaaaa ccttgtgcta taatccgcgc ccgcccggtt ttgataattt agtggaaaag
     1021 gaaaagaaat gtcgcttttt attaccgacg agtgcatcaa ctgcgacgta tgcgaacccg
     1081 aatgccccaa tgatgccatt tcccaaggcg aggaaattta cgaaatcaac cccaacctct
     1141 gcacgcagtg cgtcggacac tacgatgagc cgcagtgcca gcaggtttgc ccggtggact
     1201 gcatcctgat tgacgaagaa catcccgaaa cccatgacga gttgatggcg aaatacgaaa
     1261 agattatcca gtttaaataa attcttttta aaacatcaaa ttatgtctgt tttgaaataa
     1321 aatcaaaaaa aaacttgacg gaaaagcaag ccgctaataa actagtgttc tcttttggag
     1381 ggattcccga gcggtcaaag ggggcagact gtaaatctgt tgcgaaagct tcgaaggttc
     1441 gaatccttct ccctccacca aaattcttac ttggggcagt agcgagtaat gcgggtgtag
     1501 ctcaatggta gagcagaagc cttccaagct tacggtgagg gttcgattcc cttcacccgc
     1561 tccaaacaat taggcccatg tagctcaggg gtagagcact cccttggtaa gggagaggtc
     1621 ggcagttcaa atctgcccat gggcaccatc tctcgattat tcatttcttt aaggcttaga
     1681 tatataggat attgccatgg ctaaggaaaa attcgaacgt agcaaaccgc acgtaaacgt
     1741 tggcaccatc ggtcacgttg accatggtaa aaccaccctg actgc
//
LOCUS       DAXFZR010000151         1777 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000151 DAXFZR010000000
VERSION     DAXFZR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.152"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..198
                     /locus_tag="ACFJAS_002141"
     CDS             <1..198
                     /locus_tag="ACFJAS_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983285.1"
                     /translation="SFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDY
                     YEDNYKVNWKINNGNLIQTTN"
     gene            413..1117
                     /locus_tag="ACFJAS_002142"
     CDS             413..1117
                     /locus_tag="ACFJAS_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC3983286.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGVTGYKPVTARYGNPYR
                     ETVYIEVSDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWRTYFVKGNQAETEGVWVHNDCPYGNLSDNKSVGEGKKFTPAQK
                     KAIIQENMNRNGGVVKSDQSGEVLVRPKKSQKGITPPSNEWQIDHVQAKSKGGSNSYK
                     NAQVLSRRENIKKSNK"
     gene            1126..1398
                     /locus_tag="ACFJAS_002143"
     CDS             1126..1398
                     /locus_tag="ACFJAS_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983287.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIDEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            1565..>1777
                     /locus_tag="ACFJAS_002144"
     CDS             1565..>1777
                     /locus_tag="ACFJAS_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC3983288.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGI"
BASE COUNT          633 a          310 c          353 g          481 t
ORIGIN      
        1 agttttaatt ggagcagcta tatttttttt ggtgcatgcg taaaatctta tttcattatt
       61 tattttgaaa aatgtattaa aacaataatg gaattggata ttgaaatatc aggttttttt
      121 gaattagatt attatgaaga taattataaa gtaaattgga aaataaataa tggaaatttg
      181 atacaaacga ctaattaaat ggacaaatat aagttagatt ggacacccaa acctaaaaac
      241 tgtctgaaac tcaatttggt tttcagtaag cgtaggttgg cttaaaaacc caaccaccaa
      301 aatgccgtct gaagcggtat tcagctttca gacggcattt tgatgaatga aacaggatat
      361 tgagaactaa gttctttaaa aatcctacac ctgccctttc cacggcagca ccttggtcaa
      421 aacggcagac ggctacaaag ccattgcccg tatccgagcc ggcgagagcg tcctctccaa
      481 ggacgaggca agcggggtaa cgggatacaa acccgttacc gcccgatacg gtaatccgta
      541 tcgggaaacc gtttacattg aagtttcaga cggcatcggc aacagccaaa ccctgatttc
      601 caaccgcatc catccgtttt acagtcaagg aaaatggata caggcaggtc gtctgaaaaa
      661 aggcgacacc ctgctttccg aaagcggtgc aaaacagacg gttcaaaaca ttaccctcaa
      721 acagcagccg ctcaaagcct acaatctgac cgttgccgac tggcgtactt actttgtcaa
      781 gggcaatcag gcggaaacgg aaggagtttg ggttcataat gattgtccgt atggaaattt
      841 atcggataat aaatctgttg gcgaaggaaa aaaatttaca ccagctcaaa agaaagcaat
      901 catccaagaa aacatgaata gaaacggagg ggtagtgaag tctgaccaat ctggagaagt
      961 tttggttcgt cctaaaaaga gccaaaaagg tatcaccccc ccatcaaatg aatggcagat
     1021 tgaccatgtg caagcaaaaa gtaaaggagg ttcaaatagt tataaaaatg cacaggtttt
     1081 atccagaaga gaaaatatta agaaatcaaa taaataggta tcaatatgca atggaaattt
     1141 gaagatcctc aaaatactgc tgttattacc accagaagaa ttttgtctaa aaaaaacgga
     1201 atcttagaag tatggcatga tgctgatgat ggaatgtggc aattcttaga tggcaatgaa
     1261 attgatgaag gagatgctgt gatagttagt ttaaaagaaa ttgtcactat tgattcctca
     1321 gtcaatctct taagtaattt acctcttggt ggttacgctt ggagaaatac tatatcggaa
     1381 aaatggaatt ttgattagca agcgtaggtt aggttaaaaa aaccaacaat caaaggtcgt
     1441 ctgaaacagt gtttagtttt tcaaacgacc ttttcttcaa ttaacataga atgttgggtc
     1501 tcgacccaac ctacacgctt actaatctca aatataacct tatgaaataa tgagtattta
     1561 aaagatggaa acattagacg aacgaataaa gaatttagga aaatctcttg aagatagaat
     1621 tgacgctaat ctaattgatg ccacactaga gtacattact ttttcagaac gtttgctagc
     1681 ttttgaaacg ctatgtgatt atattgaaga tttcaatatc cagcttacgg aaaaagaatc
     1741 ccaagaaatc tcttttatta ataaggaatt tggaatt
//
LOCUS       DAXFZR010000152         1723 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000152 DAXFZR010000000
VERSION     DAXFZR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.153"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            148..927
                     /locus_tag="ACFJAS_002145"
     CDS             148..927
                     /locus_tag="ACFJAS_002145"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC3983289.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            1136..>1723
                     /locus_tag="ACFJAS_002146"
     CDS             1136..>1723
                     /locus_tag="ACFJAS_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC3983290.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAERRRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKNNTDINDITTACVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTAR"
BASE COUNT          498 a          568 c          358 g          299 t
ORIGIN      
        1 gtcggtaatg cattttttag tagtatttga aaaagtatga tgataaaaac ggttaagcct
       61 aactttttca taaaatccag cagagtaagg cagaaaaaaa cacccaaatt ggctataatc
      121 ccgacaaaca cactcaagga caacaacatg gcagcctcgc ccgaagcaaa attcaccgaa
      181 gaaaagattt tgtgggtcaa acaccacacg ccgaaactca tcactttcgc catcagccgt
      241 cccgaatcct accgttttaa agccggacag ttctcccgac tcggtttcta cgaaggtaaa
      301 ggctttattt ggcgtgccta ttccgtcgtt tccgcagaat atgccgacac gctcgaatat
      361 tttgccgtac tcatccaaga cggccccatg tcggcccttt tcgccaaaat gcaacagggc
      421 gacaccatcc tgctcgataa aaatgccact ggctttctcc tgcccgaacg cttccccgac
      481 ggcaaggatt tggtgatgct ctgtaccggc tcgggcatcg cccccttcct ttccattctc
      541 gaacaacccg aaatccggca gcgtttcgat accgtcaacc tgatacattc cgtatctttt
      601 cccgaagaat tgattttcaa cgaccggctg gccgcattgt ccgaacatcc cctggtaggc
      661 gaatacggac actcgttccg tttcgtccct gttaccaccc gtgccgccaa cccctcgggc
      721 ttaagcggaa aacgcattcc ggaactctta aaaaacaaca gcatcgaaca ggcgctgcat
      781 accaagctta ccccggaatc cacacggttt atgatttgcg gtaacccgga aatggtcaaa
      841 gacactttcc aaacgctgct cgatatgggt tacgccatgc accgcaaccg cattcccggt
      901 caaatcatga tggaaaacgg cttctaaaaa cgaacgctcc caacccctac ccacgcccac
      961 acacaaaaca aaataaaaaa cagcgatata caaaaaccaa tttgcaaaaa cagacctcac
     1021 gcctatactg ccactcacag caagcaacac aaaaaagcgg gtacaaatta gggtacaaaa
     1081 cacaagcccg aacacagaag caccccaacc aaacccaaag gcaaaggcaa acaaaatggc
     1141 ggcacacaac aaactgaccc aaaaacaaat agaagcagcc aaggcagacg gcaaacaaag
     1201 caagctggcc gacggcggcg gcttatatct gctgctgcac cccaacggca gcaaatactg
     1261 gcggatgcgc taccggcacg gcggatgcga aaaaacccta gccttgggcg tatatcccgc
     1321 cgtcagcctc aagcaggccc gcgaacttgc ccgcgccgcc cgggcgcaga ccgccgccgg
     1381 catcgacccc gtcgccgaac gccgccgcac ccgtcccggc agcgggcgca gccttcccga
     1441 aatcgcccgc gcgtggtacg gcagccgtca agggcaacgt gccgacaaca cactggaagg
     1501 cgaccgccgt tcactcgcct acctgaccga ctactacaaa aacaacaccg acatcaacga
     1561 catcaccacc gcctgcgtat caaaatttat cgaacacctc aaccgcatca acatccccgc
     1621 ctgcgcccgg cgcaccgtcc aaatcctcgc ccaaatatgg gactatgccg tccagcgcgg
     1681 catcattacc gacgaacggc gcaaccccgc aaccaccgcc cgc
//
LOCUS       DAXFZR010000153         1618 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000153 DAXFZR010000000
VERSION     DAXFZR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.154"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            166..656
                     /locus_tag="ACFJAS_002147"
                     /pseudo
     CDS             166..656
                     /locus_tag="ACFJAS_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            693..959
                     /locus_tag="ACFJAS_002148"
                     /pseudo
     CDS             693..959
                     /locus_tag="ACFJAS_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1461..>1618
                     /locus_tag="ACFJAS_002149"
     CDS             1461..>1618
                     /locus_tag="ACFJAS_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC3983291.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVG"
BASE COUNT          391 a          383 c          447 g          397 t
ORIGIN      
        1 gattctagat tcccacttcc gtgggaatga cggttcagca agcgtaggtc ggatacttgt
       61 atccgacaaa acctttaaca ttcccatcat tgcaatccat tgcagcaatg tccaaaatgt
      121 cgaattcaag aatccggcct acaaaatcat tccgagcata atactatgaa ataccgtcgt
      181 ttttaccgca atggcggcac ttactttttt acggttgtaa ccaataaacg gcagaagatt
      241 ttgaccgatg atgcggtgcg tttggcttta cggcaggcgg taatggcggt gcgcgaacgg
      301 tatccgtttg aaattttggc atgggtgttg atgcccgatc atctgcatac catatggcgg
      361 ctgccggaca atgattctgc ttattcggaa cgctggcggc aaatcaagcg gcacagccaa
      421 tatttaatcg gcggcaatct caggctttgg caaaacgctt ttgggaacat actatccgcg
      481 gtgaggccga ttttgcctgc cattttgatt atctgcattt caatccggtc aaacatggct
      541 atgtaggaca aatttccgat tggcggtttt ctacgtttca ccgttatgtc aaacagggta
      601 tttatccgca taattggggt ggcggcaatg cggacttttc tattgaatac gattgaagta
      661 atgtcggatt cgagaatccg acctacggaa aactgaaaga gcatcggctg ctggacggca
      721 ttatgcgcaa agcccgccgc aaccgcccgc tgccggaggc gcaaaccaaa cgcaaccgat
      781 atttgtcgaa gacccgttat gtggtcgaac aaagcttcgg tacgctgcac cgtaaattcc
      841 gctatgcccg ggcggcctat ttcggactga ttaaagtgag tgcgcaaagc cacctgaagg
      901 cgatgtgttt gaacctgttg aaagcggcca acaggctaag tgcgcccgct gccgcctaaa
      961 aggcggcccg gatgcctgat tatcgggtat tcaaggggga ttaagggggt atttgggtag
     1021 agttaggagt gattggtggc ggaaacagcc gaaaatctgt gtcagggttt cagctgtcgg
     1081 gagggaaggg aattttgcaa aggtctcaag atatagtgga ttaaatttaa attagtacgg
     1141 caaggcgagg caacgccgta ccggtttaaa tttaatccac tatatttccg cctgctgccg
     1201 cccgaaaagc gtaatgcgct gatggtgctg ctgcacaatc cggacgacgg ataaccggtg
     1261 aaaacgggaa atgcccgggc ggtgtcgtct gtgcctgttt ggacaagcgt tcttgagggc
     1321 ggtaaaatca aggtttgccc gaaaagggca gggcgatatg ccgcgcttgg attttgcgcg
     1381 cggtttttcg cgttggaagc aatcatgtct gtttgaatat ttaatgccgt ctgaaacgtc
     1441 cggacggacc cccgttcctt atgaaaaaac cgaccgatac cctacccgtc aatctgcaac
     1501 gccgccgcct gctgtgtgcc gccggcgcgc tgttgatcag cccgctggcg cacgccggcg
     1561 cgcaacgtga agaaacgctt gccgacgatg tggcttccgt gatgaggagt tctgtcgg
//
LOCUS       DAXFZR010000154         1613 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000154 DAXFZR010000000
VERSION     DAXFZR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.155"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            71..811
                     /locus_tag="ACFJAS_002150"
                     /pseudo
     CDS             71..811
                     /locus_tag="ACFJAS_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            815..1216
                     /locus_tag="ACFJAS_002151"
     CDS             815..1216
                     /locus_tag="ACFJAS_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983292.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
BASE COUNT          528 a          283 c          351 g          451 t
ORIGIN      
        1 agcggcatct cggaaacgat aggcgcgtca aatatttttg tcggatacaa ataaatatcc
       61 gacctacatt tctgcgcagc aaactttaca gggtattcac gatttaggaa atttaagtcc
      121 cgcagcacaa cttgcggctg caaccgcatt acaagacagt gcctttgcgg taaaagacgg
      181 catcaattcc gccagacaat gggctgatgc ccatccgaat ataacagcaa cagcccaaac
      241 tgcccttgcc gtagcagagg ctgcaggtac ggtttggggc ggtaaaaaag tagaacttaa
      301 cccggccaaa tgggattggg ttaaaaataa cggttataaa aaacctactg cccgccatat
      361 gcagactaag gcgttaggta cggtagatga aattggcaat acagtacagc aggttgggaa
      421 acagactagc ggacaaaaaa ccagcggtgg taatcctgcg attgatagcg acccctatag
      481 cccgagtagt gtgtcagctc gtatagaagc cggtaaggtg cgcagtgatt tacaaatcaa
      541 agacattttg agcaatacta ctcaaaggag taaaacaaaa ggtcccgctg ttcagtatga
      601 taaagtgggg gattacaatg acgcactaaa tgattttaat agtctgaatg ttcgaaatgt
      661 acaaacacgt cctaatggaa cgataacggg caatttacct gatgggcgtg cggttaatgt
      721 tcgtaatgat agcagtggtg gagaaccaac acttgaaata acaattagta ataaccgaaa
      781 aataaaaatc agatatggaa atacacgata aattatgaaa ttaaaaagct tagatttccc
      841 aactggctat ttctattttg ataatgcagc aataaacttt gataaagtag aagttatagc
      901 agttggttat agaaatacgg gtaaaaccat aaaaatttct attgaggatg ttattcattt
      961 tagggttgtt gatgaatcgt attttataga tacttttatg gatttaattt cggaagatgc
     1021 agatagagct ttgcttcatg aaaatggtgg tcaatctttt ttcgaacttc ttgatgagcg
     1081 ttatgcggca tggatatcga aagaaagtta ttttcctttg aatagagaat tctttaaata
     1141 ctatattttt atgtttgagc aaacattcat agaaataatt ggttctagtg caacgtattc
     1201 aattattgag agctagcgta agatgagtaa taagttgcct atctttcttt caggcagcct
     1261 gaaaataaaa ttactcgtgt aatgaaccac gcactttaaa cagcacaggt attttgatga
     1321 gaatggcatg gcaattaagt atattggttt ctacattccg tccgcaatat tcgcgccttc
     1381 cgcattgaac aatggtcaga ctttacggat atagccgtag gttggattct cgaatccgac
     1441 attttctaac agcggcattt cggaaacgat aaacgcgtca aatatttttg tcggatacaa
     1501 atatccgacc tacatctctg cgatggggta cctgtatcag tgaagggaaa ttttgtcgat
     1561 ggtaaatttc gcattggtac ggcaacaatg aaatcatttt aaattgagct aga
//
LOCUS       DAXFZR010000155         1401 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000155 DAXFZR010000000
VERSION     DAXFZR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.156"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(1..75)
                     /locus_tag="ACFJAS_002152"
                     /pseudo
     CDS             complement(1..75)
                     /locus_tag="ACFJAS_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(220..1143)
                     /gene="lpxC"
                     /locus_tag="ACFJAS_002153"
     CDS             complement(220..1143)
                     /gene="lpxC"
                     /locus_tag="ACFJAS_002153"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC3983293.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          327 a          343 c          390 g          341 t
ORIGIN      
        1 ttccggccat ttgccgtgat tcaggtaata ctcggtgacg gctgattttt gaccttcggc
       61 caaaaggatg gcttcgtcat tcccgcgcag gcgggaatcc gggtctgtcg gcacggaaac
      121 ttatcgggaa aacggtttct ttagatttta cgtcctagat tcccgcccgc gcgggaatga
      181 cggcatctgt ttgacggcat ttgtttggaa aagggggcgt tatccgcaat ttctgatgtt
      241 cagctcgtga aatgcgtcgg gcaaatcatc gctgtcggca aattccaccc attcgtaagc
      301 cgtttcgtct gccaaaactg cgcgcaaaag ggcgttgttg acggcgtgtc ccgatttgta
      361 gccttcaaac gcgccgacaa tcggatgtcc gacgatatac aaatcgccga tggcgtcgag
      421 gattttgtgg cgcacaaact catcgggata gcgcaagcct tcaggattca ggacatccgt
      481 gtcgccaatc acgatggcgt tgttcaaatt gccgcctaag ccgagattgt gggcgcgcat
      541 catttccact tcgtgcataa agccgaaagt gcgcgcgcgc gcgatttcgc cgatgtagga
      601 tttgccggcg aaatcaattt caaacgtagg cgggctgcgg ttgaaaacag gatggtcgaa
      661 ttcgatggtc agcgttacct taaaaccgtc atacggcgta aatcgaaccc atttgcccgc
      721 ttctttgatt tcgacgggtt tgaggatttt caaaaaacgc ttttgcgcct tttggtctac
      781 aacgcccgca tcttgcagga ggtaaataaa cggcaggctg gagccgtcca taatcgggat
      841 ttcgggcgcg ttcaactcaa tcagcgcatt gtcgatgccg taggcggaca gtgcggacat
      901 gatgtgttcg atggtgccga cgcgcaggcc tttgtcggta acgatggtgg atgaaaggcg
      961 ggtatcgttg atcaaataag ggttcagctt gatttgttcg cccatctcgc cgtccaaatc
     1021 ggtgcggcgg aaggaaatcc cgctgttttc aggcgcaggg tgcagggtca gcgcgacgcg
     1081 ttcgcccgaa tgcagcccga ctccggtaac gccgatggat ttcgccaaag ttctttgcag
     1141 cataaaccgc ttccttatca atggggtaag tttcggaata atacgataaa accggaaaaa
     1201 caggctatgt ttttcaatag tatttgccaa tgtatccgtt tccaacacgt aagccgcatg
     1261 aaaatgtatg gcggattaac gaaaatcagg acaaggcggc gggccgcagg cagtacggat
     1321 agtacgaaaa ggcgaggcaa cgccgtactg gtttaaattt aattcactat acatcccgtc
     1381 attcccgcgc gggcgggaat c
//
LOCUS       DAXFZR010000156         1342 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000156 DAXFZR010000000
VERSION     DAXFZR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.157"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(66..782)
                     /locus_tag="ACFJAS_002154"
     CDS             complement(66..782)
                     /locus_tag="ACFJAS_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC3983294.1"
                     /translation="MPFIIDYQCLCAINSEIKMFSGEQLGQAISEAIKRKNVSQKEVA
                     DHFGVKQPSVSGWIKNGRIDKKHLDKLIDYFSDVVTPSHFGIETFRVLKSNEQSSIRF
                     PRLNAEATCGAGTINDHYIEVVDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIE
                     NGDVMFVDTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGE
                     DLNAVRIIGRIRGAWRLSQF"
     gene            851..1087
                     /locus_tag="ACFJAS_002155"
     CDS             851..1087
                     /locus_tag="ACFJAS_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC3983295.1"
                     /translation="MAMSIQKAVDYFGNESRLARAIGVKQPTVWAWNKKGTPPPIIRC
                     VQIEKLTGGAVNRKDLRPDDWHLIWPELAGDQPK"
     gene            1167..1322
                     /locus_tag="ACFJAS_002156"
     CDS             1167..1322
                     /locus_tag="ACFJAS_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983296.1"
                     /translation="MKKQDKNRLSKKDRRLIKKAMLKAAAKGCDEVYRIAPGLKDGFE
                     LLGKQPD"
BASE COUNT          364 a          295 c          324 g          359 t
ORIGIN      
        1 aatatgcggt aataaatcag ttgttcaagt attttaatcc ggcacgctcc accagaagac
       61 gcggtctaga actggctcaa acgccatgcg ccgcgtatgc gtccgatgat gcgtacggcg
      121 tttaaatctt cgccgcgcac ggtttcggtt cggtatgagc tgttgtcgct gatgatcatc
      181 aggccgccgc cgacggtgga ttgcagccgc ttggccttaa ggccgtctat ataccaaagc
      241 aggtagaggc cgtcgccatc gaaggcttcg acggcggtat caacgaacat tacgtcgccg
      301 ttttcgatgg tgggctccat gctgtcgcca cgggctgtaa tgacttggat tttgttgagg
      361 tttccgccca gtttctcccg cgcccatgcg gcagcgacgg ttacataatc cacaacctcg
      421 atatagtggt cgttaatcgt gcctgcgccg caggtcgctt cggcatttaa gcgggggaaa
      481 cgtatgctac tttgttcatt cgattttaag actctgaatg tttcaatgcc gaaatggctt
      541 ggcgttacta cgtctgagaa ataatcaatt aatttatcta gatgtttttt atctattcgt
      601 ccatttttta tccaacctga aacgcttggc tgtttcaccc caaaatgatc ggcaacctcc
      661 ttttgactga cattttttct cttaatcgct tctgatattg cttgtcctaa ctgttcgcct
      721 gaaaacattt tgatctccga attaattgcg cataagcatt ggtaatcgat aataaaaggc
      781 ataacctata aaaggcaata gttgtattta atataagtat tagctataat gaagctattt
      841 aattgagtaa ctggctatga gtatccaaaa agcagttgat tattttggta atgaatcccg
      901 acttgcacgg gcgatcggag ttaaacaacc gacggtgtgg gcttggaata aaaaaggaac
      961 gccgcccccg atcattcggt gcgtgcagat tgaaaaatta accggaggcg cagtgaatcg
     1021 aaaagactta cgtcctgatg actggcatct aatctggccg gaattggcag gcgaccaacc
     1081 caaataaaaa gcccgtcggg tcagatgtta attgatataa ccgaattgaa gcggaagtca
     1141 tccgcaattt accggaaagg aaaaaaatga agaagcagga taaaaaccgc ctgtcgaaga
     1201 aagacagacg gctgattaaa aaggcgatgc tgaaagccgc cgccaaaggc tgcgatgagg
     1261 tttacagaat cgcgccgggt ttgaaagacg gctttgaatt acttggaaag cagcccgatt
     1321 aaatattcgt tatcggtatt tg
//
LOCUS       DAXFZR010000157         1324 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000157 DAXFZR010000000
VERSION     DAXFZR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.158"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(58..285)
                     /locus_tag="ACFJAS_002157"
     CDS             complement(58..285)
                     /locus_tag="ACFJAS_002157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983297.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFARMNGGYCFYQSVSFSLK"
     gene            320..469
                     /locus_tag="ACFJAS_002158"
     CDS             320..469
                     /locus_tag="ACFJAS_002158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983298.1"
                     /translation="MKKTLLALSLAAVSAVTVADIPESNGKGEYKVFTAYVKGKATAG
                     NLKEK"
     gene            511..807
                     /locus_tag="ACFJAS_002159"
     CDS             511..807
                     /locus_tag="ACFJAS_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983299.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPV
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            804..1043
                     /locus_tag="ACFJAS_002160"
     CDS             804..1043
                     /locus_tag="ACFJAS_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983300.1"
                     /translation="MSGVAVYLRNKNRIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVSNTTKTKTDSEKFNE"
BASE COUNT          406 a          345 c          257 g          316 t
ORIGIN      
        1 actgatatat cttttttcat agattttaaa tttcatattt ttaatatttt aggaatttta
       61 tttcaaagaa aaagaaacag attgataaaa acaatacccg ccattcattc gggcaaaacc
      121 gcacggaaca tccgttttcc cgtttccacg acaatccggt acaggacacc ccgctgccga
      181 ttccgcctgc acatccggca aaatttcata cgcaaggaaa atgttgaaaa caaaaagaac
      241 taattgcttg aaaaaagatt taattggaat gcgggtattt cccacacttt ctccatttat
      301 ttatcctcat tataaaaaca tgaaaaaaac cctgcttgca ctctcacttg cagccgtgtc
      361 cgccgttacg gtagccgata ttcccgagtc taacggcaaa ggcgaataca aagtctttac
      421 cgcttatgtt aaaggcaaag ccactgccgg caatttgaaa gaaaaataac gcacaccgtc
      481 catcggtttt cgttcaacga acgccacgat atgcgtttta aggcagagta cgcccgaagc
      541 aaaaacgatg cagacgacaa aagagggaat gcacacatcg ctaacggcgc aataggcaaa
      601 atggctgccg tgtatgccgg ttatacctac acccaaccgg tcggcgaatc cacacgtttc
      661 cgtggcggtt tggagcgtgt ccaagttaaa cgttatctgg gagaaaaatg ctgcggctac
      721 gcctgtccaa aaagcgaacg cttcaccctg caagcagatg gtgaaatccg tcaaaacctt
      781 ggcgaaggct ggtttctgaa gctatgagcg gggtggctgt ctatctccgc aacaaaaaca
      841 gaatagcaat tgcctcccaa gatgccaacc taaactccaa aggccggttc gtcagcagcg
      901 gtctgaatgt gggcaaacaa ctgaccggca gtctcggcgt agagtttgac ccatactacc
      961 gccaccgcgc aatccgcaag tctgccgaat ttgtctcgaa cactaccaaa accaaaacag
     1021 acagcgaaaa gtttaacgag tagggcttcc gcgtagccgc aacgttctaa ttcccttacg
     1081 tataaaaagc agcataaaaa tgctgccttt tcccatttcc aagggaagta ttccgtagtg
     1141 cagcttacct cattgtttca cacaaaccga aaatgccgtc ttaaaaccaa ttttcagacg
     1201 gcatttgtcc cttaaaacac gttttttcaa gcgcgaccat accaaacacc cacaggttgc
     1261 cttccaccct tcgtacaacc tttgctccat caaacatcca cttcctaaat actgatgtat
     1321 actg
//
LOCUS       DAXFZR010000158         1317 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000158 DAXFZR010000000
VERSION     DAXFZR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.159"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1072
                     /gene="mafB"
                     /locus_tag="ACFJAS_002161"
     CDS             <1..1072
                     /gene="mafB"
                     /locus_tag="ACFJAS_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3983301.1"
                     /translation="PPGGARDIYSYYVKGTSTKTKINTVPQAPFSDRWLKENAGAASG
                     FFSRADEAGKLIWENNPNQNWWGNRMDDVRGIIQGAANPFLTGFQGLGVGAITDSAVN
                     PVTYAAARKTLQGIHNLGNLSPEAQLAAASLLQDSAFAVKDSINSARQWADAHPNITA
                     TAQTALSAAEAAGTVWGGKKVELNPAKWDWVKNTGYKKPAARPMQTVDGEMAGKNKPP
                     KPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGTIQDRIRWTKSKFPTEKSLNGHFKAH
                     GKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHRRVRYDINNNIYVLANPKTFKIK
                     TMFKPNLGKKYYDGEFKKDMGN"
     gene            1038..1244
                     /locus_tag="ACFJAS_002162"
     CDS             1038..1244
                     /locus_tag="ACFJAS_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC3983302.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
BASE COUNT          450 a          279 c          290 g          298 t
ORIGIN      
        1 gccccccgga ggagcaaggg atatatacag ctactatgtc aaaggaactt ctacaaaaac
       61 aaagataaac actgttccgc aagccccttt ttcagaccgc tggctaaaag aaaatgccgg
      121 tgccgcttcc ggttttttca gccgtgctga tgaagcagga aaactgatat gggaaaacaa
      181 ccccaatcaa aattggtggg gtaaccgtat ggatgatgtt cgcggcatca tccaaggtgc
      241 agccaatcct tttctaacgg gttttcaggg attgggagtt ggggcgatta cagacagcgc
      301 ggtaaacccg gtaacctatg cggcagcacg gaaaacttta cagggtattc acaatttagg
      361 aaatttaagt ccggaagcac aacttgctgc cgcgagccta ttacaggaca gtgcttttgc
      421 ggttaaggac agtatcaatt ccgccagaca atgggctgat gcccatccga atataacagc
      481 aacagcccaa actgcccttt ccgcagcaga ggctgcaggt acggtttggg gcggtaaaaa
      541 agtagaactt aacccggcca aatgggattg ggttaaaaat accggctata aaaaacctgc
      601 tgcccgacct atgcagactg tagacgggga aatggccggg aaaaataagc caccgaaacc
      661 aagtacgcag caacactcta cacactctga taacaatatc ggcttacctg ccccatatgt
      721 taaacctgat acatctattt ctccgacagg aacaattcaa gaccgcatca gatggacaaa
      781 atccaagttt cctactgaga aatctttaaa tggacatttc aaagctcatg gaaaagaatt
      841 tggcgatata accattgaag actaccaaaa aatggcgtct gatttgttat caaaacagac
      901 atcggacaag atattaggtt atcagacgga acatagacga gtgcgctatg atatcaataa
      961 caatatctat gttttggcca atccaaaaac attcaaaatc aaaacaatgt ttaaaccaaa
     1021 cttaggaaag aagtattatg atggagaatt caaaaaagac atgggaaatt gacggagaaa
     1081 tatggctaca ttgtcctgtt tgcggaactg aagttatgga ctatgatatc tgtgacgttt
     1141 gtcagtggca aaatacagga gaaactaata tagatggtgg tcctaatgaa atgacacttg
     1201 cggaggcgaa agaagcttac gcaaaaggct taccaatcag ataaataagc acctagagaa
     1261 accaatgatg acggaacccc atggttaccc atcaaataat ttacctgtta aaatcat
//
LOCUS       DAXFZR010000159         1213 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000159 DAXFZR010000000
VERSION     DAXFZR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.160"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(208..381)
                     /locus_tag="ACFJAS_002163"
     CDS             complement(208..381)
                     /locus_tag="ACFJAS_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983303.1"
                     /translation="MTTAVKPPADLVRPCPKLPHLEGNTGADVLPWSLQVIGLYKDCK
                     ARHGALVRALGAD"
     gene            complement(365..706)
                     /locus_tag="ACFJAS_002164"
     CDS             complement(365..706)
                     /locus_tag="ACFJAS_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983304.1"
                     /translation="MNGLIKNPKLIAVSVCAVFVAGAWQYDRAAQYRRGYGAAMLEVS
                     ERLKAAAVEHAEHARKSSAAYQAQKAAREEKERVRYVQTLKIIEKPVYRNACFDADGV
                     RELNAAVDDGG"
     gene            complement(690..848)
                     /locus_tag="ACFJAS_002165"
     CDS             complement(690..848)
                     /locus_tag="ACFJAS_002165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983305.1"
                     /translation="MMNPTEFLKARIAEWEAKSKEAGGNADFKAFEFAESEIKNYKAM
                     LKTYERPD"
     gene            complement(848..>1213)
                     /locus_tag="ACFJAS_002166"
     CDS             complement(848..>1213)
                     /locus_tag="ACFJAS_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC3983306.1"
                     /translation="GHCLGKAGRAVIRDWYRAKAWAMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAE
                     GRYRLTAAAATGAQGAGEA"
BASE COUNT          231 a          387 c          327 g          268 t
ORIGIN      
        1 gcctgcccat agggtacggc attttcattt ccgcccatta ttggtgcgcc tgataatttt
       61 gtataacgcc cccttcgcgc aaacaaaacg gcaaaaattt gccgcgtccg cccgatatgt
      121 aaaaaatcgg ggggaatgcc gacgcttccc ccgatgcggc cgtcaaccga aagttgaccg
      181 ttcggttgca aacttccggt tgacaactca atccgcgccc aacgcccgta ccagcgcgcc
      241 gtgccgcgcc ttgcagtcct tgtacaagcc gatgacttgc aacgaccacg gcagcacgtc
      301 cgcgcccgtg ttcccttcaa ggtgcggcag tttcgggcag ggccgcacca aatcggcggg
      361 cggcttaacc gccgtcgtca acggcggcgt tgagttcgcg cacgccgtca gcatcaaaac
      421 aggcattgcg gtacacaggt ttttcaatga ttttaagcgt ttgcacatag cgcacccttt
      481 ctttttcctc acgcgccgcc ttttgcgcct gatacgcggc ggacgatttg cgggcgtgtt
      541 cggcgtgttc gaccgcggcg gctttgaggc gttccgaaac ctccagcata gccgcaccgt
      601 atccgcgacg gtattgcgcg gcgcggtcgt attgccacgc gccggcaacg aacacggcgc
      661 aaaccgacac cgcaatcagc ttcgggtttt taatcaggcc gttcataagt tttcaacatc
      721 gctttgtagt ttttgatttc gctttcggca aactcaaaag ctttaaaatc ggcgtttccg
      781 cccgcctctt tgcttttggc ttcccattcg gcgatacgcg ctttcagaaa ctcggtagga
      841 ttcatcatta cgcctctccc gcgccttgcg cgccggtggc ggcggcggca gtcaaccggt
      901 aacgcccttc ggcgggggac gaaggcacag gtttgccgcc aatcagcttg gacggccaga
      961 aatagccgtc aatgtccgca gggtcaaacg ggatgatgga taccatattg ccctgattgc
     1021 cgcccaagcc caagattctg ccttccgcgt ctttgccgac aacgaagaac acgtgtccgc
     1081 cgccccggcg cggtttgacc gcgatgcagc cgtatgcggg ggcttcgagt ttcgtcaaac
     1141 ccgacattgc ccaagccttg gcgcgatacc agtccctgat gaccgcgcgt cccgctttgc
     1201 ccaggcagtg tcc
//
LOCUS       DAXFZR010000160         1188 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000160 DAXFZR010000000
VERSION     DAXFZR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.161"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            130..276
                     /locus_tag="ACFJAS_002167"
     CDS             130..276
                     /locus_tag="ACFJAS_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983307.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            complement(653..1102)
                     /locus_tag="ACFJAS_002168"
     CDS             complement(653..1102)
                     /locus_tag="ACFJAS_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC3983308.1"
                     /translation="MTDNREFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          367 a          292 c          181 g          348 t
ORIGIN      
        1 aaggtcgtct gaaacccatt ttcagacgac ctttttccac ttcgcaaaac aagaattccc
       61 acccctctcc ccaaacatcg cccaaaccag taaattcccc atatttcaac aactccttgg
      121 aagcaaacca tgtcccgtat ctacctaccc ctcctatttc cgccccacat cgtcgaacgc
      181 ggccttttgt attttcagca ggacaaggtt cgcgatgtcc aaaaaatttc cttagggcgt
      241 tatcgggcga aggtattcga ttcggaaaac tattaggcat ggttgaagct ggatagtgat
      301 ttgtacatta aggacggggc tgcgattgcc cttatgtcta aaggtgcaaa catgccttaa
      361 attactaagc ctataacgaa ttggaaacaa aatgccgtct gaaaacatct tcagacggca
      421 ttataaaatc tgttcacctt ttcagatgag taatgtacac ccctatacaa tttttgctac
      481 catgcaccat aaatccacgg ctaaagataa ttgggtttat tcttttatcc agaaacaatc
      541 cagatacgga tcacgggtca taactatagg cttaatatta cacgattctc attccatcaa
      601 ggcggaaaac cgcacaaata ctgaaacact atcgatcgat ttgtaaacaa gcctacttaa
      661 gtaacttgca gtccttatca tttcctttaa aataatccag cccgtcacta cacgaactgg
      721 cggacttctt gcaaataaag gttactagat tttcattcat cttaataata aaaggatttt
      781 tatctttatc tatggctacc gccttcaaca tgaatttact gtctaaagcc ccgcgcgcga
      841 ttccattcaa acggatacaa aagccttctg cctctttaat cggcaaactt ggccatttgg
      901 tagatgtttg tttaaatctc ccattctgca gataaaactt ttccataaaa tgtgcatttt
      961 ctaacaaggc tgcccgcact gcatttatct ttgctttctc aacataattg cgatagctcg
     1021 gataaacaat taaagcaagt acagacaata tcaagaccac tgatattaat tcaaccagcg
     1081 taaactcccg attatcagtc attactttac ttccaataag aacagattat tcaacatatt
     1141 tctttgaaca gacttactat cccattcagc agtatgcata tttcccac
//
LOCUS       DAXFZR010000161         1144 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000161 DAXFZR010000000
VERSION     DAXFZR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.162"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            451..816
                     /locus_tag="ACFJAS_002169"
                     /pseudo
     CDS             451..816
                     /locus_tag="ACFJAS_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            843..1144
                     /locus_tag="ACFJAS_002170"
                     /pseudo
     CDS             843..1144
                     /locus_tag="ACFJAS_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          332 a          323 c          279 g          210 t
ORIGIN      
        1 gcgggtggcg gccgggcggt ttgtcgctat gatacggaag ttccgatata caagggcggg
       61 cttcagcccg ccaaatccac caactccgcc gaatccaccg attcccgccg ccgtcattcc
      121 cgcataagcg ggaatccaga cctgtcggca cggaaacctg catcccgtca ttcccacaaa
      181 agtgggaatc cggaacgaaa aatctcaaga aaccgtttta cccgataagt ttccgcactg
      241 acagacctgg attcccgcct acgtgggaat gacggtatat cggtttccgt tcggacaaat
      301 gtctgcgcag gaataaatgg ccgcgtttga attctcggtt aattccattt attagccgct
      361 tgcttctgtt tgcatgaaag ccgggattat tgaataaaat acatttgcac ggccggttag
      421 gtttgcccgc cggaaattta aacaccgcca aaaaaatcag ccgttaccga gtattacccg
      481 aatcacggcg aatggtcgga aaacaacgct tctgccggcg tggcatcctc cgcctccgac
      541 atcaaaggca aatatgttca aagcgttacg gtcgcaaacg gcgtcgtcac cgccacaatg
      601 aaatcagacg gcgtaaacaa agaaatcaaa ggcaaaaaac tctccctgtg gggcaggcgt
      661 gaagacggtt cggtaaaatg gttctgcgga cagccggtta agcgcgacaa cgccaaagcc
      721 gacaacgacg acgtcaaagc cgacgccgcc gacaaagaca aaatcgaaac caagcacctg
      781 ccgtcaacct gccgtgatac gtcatctgcc aaataaggca aattaggcct taaatttcaa
      841 ataaatcagc cctcaccggg cattacccga ataacggcga atggcccaaa gacaacgact
      901 ctgccggcgt ggcatccgct tcaacaatca aaggcaaata tgttcagaaa gttgaagtca
      961 caaacggcgt cgttaccgcc acaatgaaat cagacggcgt aaacaaagaa atccaaggca
     1021 aaaaactctc cctgtgggcc aagcgtgaag acggttcggt aaaatggttc tgcggacagc
     1081 cggttacgcg cggcgacaac gccgccaacg acaccgttgc cgacgccgcc gacaacgacg
     1141 gcaa
//
LOCUS       DAXFZR010000162         1080 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000162 DAXFZR010000000
VERSION     DAXFZR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.163"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            48..287
                     /locus_tag="ACFJAS_002171"
     CDS             48..287
                     /locus_tag="ACFJAS_002171"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFC3983309.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSKRCSCTGFTG"
     gene            347..1000
                     /locus_tag="ACFJAS_002172"
                     /pseudo
     CDS             347..1000
                     /locus_tag="ACFJAS_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
BASE COUNT          335 a          200 c          246 g          299 t
ORIGIN      
        1 tgtatattta gtgatgtgat aagtatagtt tattttttga ggtttttatg aatttacctt
       61 ttcgggcaat ggtgtctgat ttaggtggca cacttttaac acctgaacat ttagtgggtg
      121 atcttaccat tgatactctg agagtattag agcaaaaagg cgtagatatt attttggcaa
      181 caggacgcaa tcataccgat atgtcatcca ttcttggaaa aatcggtgcg gaacgtgcgg
      241 ttatgattac atcaaaacgg tgctcgtgta cgggatttac agggtaattt gctttatagc
      301 aatagtttgc ctgaagaatt agttctcgag ctttacaaaa catcatatgt gggaaatctt
      361 attcagacac caagattttg tcgccatcaa taaacctcaa ggcatatccg tccaccgttc
      421 cggcggcgaa gtcagtctga cggcaacact tgcggcacag ttgggcgtag aaaaggtatg
      481 gttgctgcac aggttggaca agcaggccgg aggcatattg ctgtttgccc ttaatcccca
      541 aagtgccgct gttcttgccg cacagtttgc cgaaaggaaa atgaagaaaa gctatttggc
      601 tttgtccgac cgcaaaccgt ccaaaaaaca aggatggata aaaggcggca tggaaaaatc
      661 caggcgcggg atgtggaagc tgacgcgcaa tatggaaaat attgccgtta cccggttttt
      721 cagtatccgc atcagtgaaa aaatgcggct gttcatcctt gagccacata cgggtaagac
      781 acatcagttg agggtggtaa tgaagagtct gggcagctct atatttgggg acagcctgta
      841 tagaggaaca gaatccgaaa ctatgttcct gtatgcgtga aaaatacaat ttacctatca
      901 acaacaagaa atcgaaatta ccgcaccttt gaaaaatgca tggcaggccg aaaatatctc
      961 tcatgcactg gaagaatttt atatggcaaa agcaggttaa tagatataaa tcaattaaaa
     1021 caatatgata aatattttta taaaattttt tcaaaaaacg gttgaccgca aatctcttta
//
LOCUS       DAXFZR010000163         1074 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000163 DAXFZR010000000
VERSION     DAXFZR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.164"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..214)
                     /locus_tag="ACFJAS_002173"
     CDS             complement(<1..214)
                     /locus_tag="ACFJAS_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC3983310.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAH"
     gene            complement(205..588)
                     /locus_tag="ACFJAS_002174"
     CDS             complement(205..588)
                     /locus_tag="ACFJAS_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFC3983311.1"
                     /translation="MNGAEFTLTPQNKKQVMRSIWDSPDGWFENGNLEITIRPRKSKR
                     SIEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(585..890)
                     /locus_tag="ACFJAS_002175"
     CDS             complement(585..890)
                     /locus_tag="ACFJAS_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFC3983312.1"
                     /translation="MSSAIRKAAKGGQCTPNIAGVCNDNPETVVLCRFPGETHGAGLK
                     SGGLGAGFGCGCRRGAIDGRGAGLSREDKEFYMRRSQLRTIRRLEALGVVGVKGRLK"
     gene            complement(883..>1074)
                     /locus_tag="ACFJAS_002176"
     CDS             complement(883..>1074)
                     /locus_tag="ACFJAS_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFC3983313.1"
                     /translation="VTLDALQGVAYHNDRQVRRIAADYADEPVAGGGLAVEVGELEME
                     QTDAADEGWDFIGQEGWDV"
BASE COUNT          242 a          333 c          203 g          296 t
ORIGIN      
        1 tatgtgcatt atattgtttc ttaaatattt ttaacttcaa ataccccaac ctgcctttgt
       61 ataagaaaac ctccttgccg gaaaatccgg tattccaaga tttgtaatgc aaaccgctat
      121 tgaaatactt tcttggacgc tccgcccaat ataactttcc cgtattgtca tcatatctta
      181 ataattcttt taaaacctct tgtgttaatc ccataaaaca ccatgctcca tagatgccca
      241 agatataatc ttttcttgat attcagacat ttcccgaact gataattttg tcgttgatat
      301 acccattaat tgcccgttag gcatttcaat acatccaata aattttcttt ttaaaaattc
      361 atgccataca tcttgactaa accttctccc atcgataaaa actttttctg aaatttcacg
      421 atacaaaaac catagccgcc tgttctgctc gatgctccgt tttgacttgc gcgggcggat
      481 tgtgatttca aggttgccgt tttcaaacca cccgtccggg ctgtcccaaa tcgaccgcat
      541 aacctgcttt ttgttttggg gcgtcagtgt aaattccgcc ccgttcattt cagacggcct
      601 ttcacgccga caacccccag tgcttcaagg cggcgtatcg tgcgcaattg cgaacggcgc
      661 atataaaatt ccttgtcttc gcggcttagg cctgcgcccc tgccgtcgat cgcgccgcgg
      721 cggcaaccgc acccgaaacc cgcgcccaaa ccgccgcttt tcaatcccgc cccgtgcgtc
      781 tcgcccggga aacggcacaa cacgaccgtt tcagggttgt cattgcacac gcccgcgatg
      841 ttcggcgtgc attgcccgcc tttggcggct ttgcgtatcg cgctagacat cccaaccctc
      901 ctgtccgatg aaatcccaac cctcgtctgc cgcatccgtc tgttccatct ccaactcccc
      961 cacctccacc gccaaaccgc cgcctgcgac cggctcgtcg gcgtaatcgg cggcaatgcg
     1021 ccgcacctgc ctgtcgttgt gataggcaac gccttgaagc gcgtccagcg taac
//
LOCUS       DAXFZR010000164         1055 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000164 DAXFZR010000000
VERSION     DAXFZR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.165"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..265)
                     /locus_tag="ACFJAS_002177"
     CDS             complement(<1..265)
                     /locus_tag="ACFJAS_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983314.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKK"
     gene            complement(302..565)
                     /locus_tag="ACFJAS_002178"
     CDS             complement(302..565)
                     /locus_tag="ACFJAS_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983315.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(585..833)
                     /locus_tag="ACFJAS_002179"
     CDS             complement(585..833)
                     /locus_tag="ACFJAS_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983316.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            complement(794..>1055)
                     /locus_tag="ACFJAS_002180"
     CDS             complement(794..>1055)
                     /locus_tag="ACFJAS_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFC3983317.1"
                     /translation="MKTKHPFTVPLSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAP
                     HPDHISIKFAHNAIRRLGYDGSTPANPNTPCTDSATSSPTSA"
BASE COUNT          160 a          218 c          374 g          303 t
ORIGIN      
        1 tttttttgac tgcccggcgg caggtttcgg taaggacggc ggcaaatcgg gcttcgtctg
       61 ccggtttgcc gccgaaaaaa ataaaatcgt aaagggtgat gccgttttcg gaatggcggt
      121 aatgcccgcc cgggcattcg ccgtctgtct gcctgtggat tttggcaatc aggcggggat
      181 agcggcaatg ggtaatgaaa cggcttgcgc cgtcttgccc gataatccat tcggggtagc
      241 ggttgaatag tgcggctctg ttcattttgt tgagggataa agcccctcgc ggggcttgtg
      301 gtcaggcaaa tttgaatatc agtgccaaca cggcggcgat ggcggtaacc agaccggtgg
      361 ccgccatcat gggataccag cgggactctt gggctatttt tacggattcg gcgttgattt
      421 tgtgcgcgtc ggcaatgatt ttggcgattt cggcatctat ttttcttaac tcggtgcgtt
      481 ttgtttcttg ttcgattatg tttattggat ttcctttcgt ttttggcggt tgccgccgct
      541 tgtcggatgg caggatgtct gccatgtatg tatgttaata ccttttaggt tttattgcaa
      601 gtgttttggg cggcggcttc gtatgcttgg cggtggcggc ggccgtacca ttcggcgagt
      661 tcgcgccgct cttggaggcg gtagctgtcg gcggtcaggt agtggtgcag gcttgagaag
      721 ccggcgcgtt ggagggcggc gcggctgatg tggccaaggg cgaggtctgt tgtgagggtg
      781 tcttttccgg cggtcaggct gatgttggtg aagaggtcgc ggaatccgtg catggtgtgt
      841 ttggatttgc cggggtgctg ccgtcatagc ccagtcgtcg gatggcgttg tgggcgaatt
      901 tgatgctgat gtggtcggga tggggggcgg gtttgcgccg tgggcggatg cctgggaaaa
      961 ggtggatgtt gtcgccggtc tgtgtgtgca gctctcggag tatttctact gcccagtccg
     1021 acagtgggac ggtaaagggg tgtttggtct tcatg
//
LOCUS       DAXFZR010000165         1020 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000165 DAXFZR010000000
VERSION     DAXFZR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.166"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..397)
                     /locus_tag="ACFJAS_002181"
     CDS             complement(<1..397)
                     /locus_tag="ACFJAS_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3983318.1"
                     /translation="MRNTVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLNQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTDKQDAK
                     LIAQYCRSAQESELVKRQKPTDEQYRLSRM"
     gene            477..706
                     /locus_tag="ACFJAS_002182"
                     /pseudo
     CDS             477..706
                     /locus_tag="ACFJAS_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(743..>1020)
                     /locus_tag="ACFJAS_002183"
     CDS             complement(743..>1020)
                     /locus_tag="ACFJAS_002183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983319.1"
                     /translation="GILQRSSGLYYKIWEGGWRMPALPFFRTSIPMLVQNRMESIFNH
                     NKDAIFLEGWSLGDAAMIMELLWQNISTSRRELTKLFLYKNFWGCTR"
BASE COUNT          276 a          227 c          200 g          317 t
ORIGIN      
        1 tcatccgtga aagcctgtat tgctcgtccg taggcttctg cctctttaca agctcgcttt
       61 cctgcgccga ccggcaatac tgcgctatca gttttgcatc ctgtttgtct gttttggttc
      121 gcttgaacct gctttctgca tacttgctta ttttcagcgg gttcactacg taaacgctgt
      181 aatactgcgc gaagtagtcg gcaacttctt cataatagtt tcccgttgcc tccatgcaga
      241 tatgcagatt ctgacatccc aagcttttca accggtccga aaactgattt aaaccttttg
      301 aatcgttgtc aaactttgcc gaatattcgg cattgccgac aatggccgtt gcgtcaaaag
      361 tcaacttgga tatgtccaat cctacggtgt tacgcatggg attaccctta tatattgcgt
      421 ccctaagaag gggcgattaa caaaaattaa tgccctttct tagggacgca atatataagg
      481 tttataccgt cgttgtccct aatgcgcaat cagcgacatt gttgccaatt atccgaaaga
      541 aagttaagcc tgatggcatt gtatacacgg atacctttcg tagttatgat gtgcttgatg
      601 ttagtgaatt tagccattta cgtaagttta acggcattcc caaagagcat ttggggctat
      661 atttaaagaa atgccaatgg catttgaaat agaatctcaa atttagattt taagacaatt
      721 agttaatggg aatttggtct ggttatctag tacaacccca aaagttttta tataaaaaca
      781 gtttggtaag ttctcttcgt gaagtgctta tgttttgcca caaaagttcc ataatcatgg
      841 cggcatcacc caagctccat ccttccaaga aaatggcatc tttgttgtgg ttgaaaatcg
      901 actccatacg attttggaca agcataggga tagatgttct gaaaaacggc agcgcgggca
      961 ttctccaacc accttcccaa atcttataat acaaacccga agacctttgc aaaattcccc
//
LOCUS       DAXFZR010000166         1010 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000166 DAXFZR010000000
VERSION     DAXFZR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.167"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..>1010
                     /gene="tuf"
                     /locus_tag="ACFJAS_002184"
     CDS             <1..>1010
                     /gene="tuf"
                     /locus_tag="ACFJAS_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC3983320.1"
                     /translation="KKFGGAAKAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVD
                     CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCD
                     MVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALEGDAAYEEKIFELATALD
                     SYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTT
                     CTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLS
                     KEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEKGVEMVMPGENVTITVELIAPIAMEE
                     GLR"
BASE COUNT          272 a          284 c          257 g          197 t
ORIGIN      
        1 aaaaaattcg gcggcgctgc aaaagcttac gaccaaatcg acaacgcacc cgaagaaaaa
       61 gcacgcggta ttaccattaa cacctcgcac gtagaatacg aaaccgaaac ccgccactac
      121 gcacacgtag actgtccggg tcacgccgac tacgttaaaa acatgattac cggcgccgca
      181 caaatggacg gtgcaatcct ggtatgttct gctgccgacg gccctatgcc gcaaacccgc
      241 gaacacatcc tgctggcccg tcaagtaggc gtaccttaca tcatcgtgtt catgaacaaa
      301 tgcgacatgg tcgacgatgc cgagctgttg gaactggttg aaatggaaat ccgcgacctg
      361 ctgtccagct acgacttccc cggcgacgac tgcccgatcg tacaaggttc cgcactgaaa
      421 gccttggaag gcgatgccgc ttacgaagaa aaaatcttcg aactggctac cgcattggac
      481 agctacatcc cgactcccga gcgtgccgtg gacaaaccat tcctgctgcc tatcgaagac
      541 gtgttctcca tttccggccg cggtaccgta gtcaccggcc gtgtagagcg aggtatcatc
      601 cacgttggtg acgagattga aatcgtcggt ctgaaagaaa cccaaaaaac cacctgtacc
      661 ggcgttgaaa tgttccgcaa actgctggac gaaggtcagg cgggcgacaa cgtaggcgta
      721 ttgctgcgcg gtaccaaacg tgaagacgta gaacgcggtc aggtattggc caaaccgggt
      781 actatcactc ctcacaccaa gttcaaagca gaagtgtacg tattgagcaa agaagagggc
      841 ggccgccata ccccgttttt cgccaactac cgtccccaat tctacttccg taccactgac
      901 gtaaccggcg cggttacttt ggaaaaaggt gtggaaatgg taatgccggg tgagaacgta
      961 accattactg tagaactgat tgcgcctatc gctatggaag aaggtctgcg
//
LOCUS       DAXFZR010000167          982 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000167 DAXFZR010000000
VERSION     DAXFZR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.168"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..518
                     /gene="mafB"
                     /locus_tag="ACFJAS_002185"
     CDS             <1..518
                     /gene="mafB"
                     /locus_tag="ACFJAS_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3983321.1"
                     /translation="TTVTRKTLDGQIAGKNKPIKPSSGDYVLPVGRQIIDPTRTSFSQ
                     ATVSYQKRGANYNYDSVVAAMNEKKSWVGDRVDVVNMPDGAPTSMDNTRIMAAREAGV
                     KVEANVHNFNDRLSSKERIRFKHDGIEPQTWGEAIQLRIRKQETQKGVPEGWSKRFPN
                     GSIYDVKVLRK"
     gene            515..886
                     /locus_tag="ACFJAS_002186"
     CDS             515..886
                     /locus_tag="ACFJAS_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC3983322.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          341 a          162 c          203 g          276 t
ORIGIN      
        1 aaacaactgt tacccgaaag actttagatg ggcaaatagc tggcaagaac aagcctatta
       61 aaccttcttc aggagattat gtcttgccgg taggcagaca aatcatagat cctactcgta
      121 catctttttc acaagctaca gttagctatc agaagcgtgg tgcaaattac aactatgatt
      181 ctgttgtagc tgcaatgaac gaaaaaaaat catgggttgg cgacagggtt gatgttgtta
      241 atatgcctga tggagcacct actagtatgg ataacacgcg tattatggca gcacgtgaag
      301 caggggtaaa agtggaagcg aatgttcata attttaatga ccgattatca tcaaaagaga
      361 gaatcaggtt taagcatgat ggtattgagc ctcaaacttg gggagaagct atccagctac
      421 gaattagaaa gcaagaaaca caaaaaggag ttccagaagg gtggagcaaa agatttccta
      481 acggaagtat ttatgatgta aaggtactta ggaaatgata aaacaaaata gttttgttcc
      541 gtatcctgaa gcaatgcttc ctaaaggatt taaatatccg caaagttatt taaaattagc
      601 tcaatccact catgccatta actacgatga acaatattct tttccttggt ggtttgaaaa
      661 tgcagaaagc aatatatcag aagtaattga catttatttt gaaataactg gcattccaaa
      721 cctattacct tttgctagaa accaagagtg ggctgcctgt tttgatattt cagataaatc
      781 aggtaatcct aaaattatag tagttaattt agataataca aaatattacg agacttttga
      841 aaattttgat acttggctaa aagaagctga aaatgatggt tggtagcagc cgtaggtcgg
      901 attctcgaat ccgacatttt ccaacagcgg catttcggaa acgatagacg cgtcaaatat
      961 ttttgtcgga tacaagtatc ca
//
LOCUS       DAXFZR010000168          918 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000168 DAXFZR010000000
VERSION     DAXFZR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.169"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..120
                     /locus_tag="ACFJAS_002187"
     CDS             <1..120
                     /locus_tag="ACFJAS_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3983323.1"
                     /translation="KYNGIDRKPFPPLLRECEFRLNFGTPSRQLKILRDWCGI"
     gene            394..720
                     /locus_tag="ACFJAS_002188"
                     /pseudo
     CDS             394..720
                     /locus_tag="ACFJAS_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          257 a          227 c          231 g          203 t
ORIGIN      
        1 aaatacaacg gaatcgatcg taaacctttc ccgccgttgt tgagggaatg cgaatttcga
       61 cttaacttcg gcacaccgtc ccggcagcta aaaatcctgc gggattggtg tggaatttag
      121 ggataatcta gtacagccac aaaaataaaa tagggaggga tgtcatgata tttgaaaaat
      181 caataaacgg ctggaaatac cgcgccgtaa tggaaatcag aaccacgcaa aaggagcttg
      241 ccttgaacag cctttataca acagacagga ttgaatagtc atgacaaaac ctgcgacgag
      301 taactgtcaa tctttcgatt ccgtcctcat acatcctaag cgtctttgca aattctgttc
      361 agttatacca tttatacaga caggagcaaa ccaatgacga agttatacgc agaaatcgcc
      421 gagatggagg cgcaggacga cggcacggtc aaagtttggg gttatgcctc aagcgaggcg
      481 gtcgattcgg acggcgaagt catcgcggcg gcggctatga aggcggcgat tcccgattat
      541 atgaagttcg gcgaggtgcg cgagatgcac ggcagcaacg cggcgggaac ggcgattgaa
      601 atcaacgtgg aagacgacgg cagaaccttt ttcggggcgc atatcgtcga tcccgttgca
      661 gtggagaaag tcaaaacggg cgtttacaag ggcttttcca tcggcggcag cgttaccgct
      721 gtctgcttag gttgaatcgg cattcgtacg attacggcac accgcctaat ctgtacctgc
      781 gtctgttgcg cccaatacca aaggctacct gaaaatctcc caaacttttc aggtagcctt
      841 atcacgtttt caactgccgc cacacgccca aagaccgcct gaaacccttt ttcagacgtt
      901 ccgctctgat acacccaa
//
LOCUS       DAXFZR010000169          876 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000169 DAXFZR010000000
VERSION     DAXFZR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.170"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            178..738
                     /locus_tag="ACFJAS_002189"
     CDS             178..738
                     /locus_tag="ACFJAS_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC3983324.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT          243 a          293 c          184 g          156 t
ORIGIN      
        1 aatctggtac agacccaaaa acaaagcacg gataacaccg cgctttttca tccgccctgt
       61 tccgcccgcc cgttcgtcaa ccgcctgcaa acccaccgtt taccggcagg aaatgccata
      121 aatccccgcc tatgttagga taaacgcctt taccggccca cacaaaggat tatcatcatg
      181 aaaaaaatca tcttcgccgc gctcgcagcg gcagccgtcg gcactgcctc cgccacctac
      241 aaagtggacg aatatcacgc caacgtccgt ttcgccatcg accacttcaa caccagcacc
      301 aacgtcggcg gtttttacgg tctgaccggt tccgtcgagt tcgatcaagc aaaacgcgac
      361 ggcaaaatcg acatcaccat tcccgtcgcc aacctgcaaa gcggttcgca acacttcacc
      421 ggccacctga aatccgccga catcttcgat gccgctcaat atccggacat ccgcttcgtt
      481 tccaccaaat tcaacttcaa cggcaaaaaa cttgtttccg ttgacggcaa cctgaccatg
      541 cgcggcaaaa ccgcccccgt caaactcaaa gccgaaaaat tcaactgcta ccaaagcccg
      601 atggcggaaa ccgaagtttg cggcggcgac ttcagcacca ccatcgaccg caccaaatgg
      661 ggcgtggact acctcgttaa cgccggtatg accaaaaacg tccgcatcga catccaaatc
      721 gaagctgcaa aacaataagc cttgatgcga atcagcacaa atgccgtctt catcaaaccg
      781 ccctgccaac cgtaagccga tatggcggag cacaacccgc cgaaagatat tttggcggca
      841 ggcggggctt gccggaaagt ccggaaagta acgacc
//
LOCUS       DAXFZR010000170          862 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000170 DAXFZR010000000
VERSION     DAXFZR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.171"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(114..>862)
                     /locus_tag="ACFJAS_002190"
     CDS             complement(114..>862)
                     /locus_tag="ACFJAS_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983325.1"
                     /translation="LQDMRYACNQELINFAEHIVKQVQRLGLYCNTDDPENGESVLFA
                     CREASQAVAQWTKDFDDLSPNQRQLVLRPLSNLFAAYEEFLKDAPARLIAEVSAYSLA
                     VRVAKKAMAFLELDGGLISAVGKVVNGADSRAEARRLKMPYAEFTGRILHAANLLYDV
                     GIQADKELSAMYGRPLNPVRPRRISDVRRPMMKMLVADKGGALVRAVKDSEDVIRHCD
                     NGAGFSCFNWTEHFKRTANLISLMHREAAA"
BASE COUNT          155 a          259 c          249 g          199 t
ORIGIN      
        1 gcgctgaaaa acgcttgttc cgccgcgccg gcagccgtgc cgcttatgcc tgatgccgtg
       61 tttgaagcct tttgtgccgg aatcgtgata cacgcaggtc cggatgtgcg tcctcatgcc
      121 gccgcttccc tgtgcatgag gctgatcagg tttgccgtcc gtttgaaatg ttcggtccag
      181 ttgaagcagc tgaagccggc gccgttgtcg caatgccgga tgacgtcttc cgagtctttt
      241 acggcccgga ccaacgcgcc gcctttgtcc gcaacaagca ttttcatcat cggccggcgc
      301 acgtcgctta tccgtcgggg gcgcacggga ttcagcggcc tgccgtacat cgccgaaagc
      361 tccttgtccg cctgtatgcc cacatcgtaa agcaggttcg cggcgtgtag gatccggcct
      421 gtaaattccg cgtacggcat tttcaggcgg cgggcttccg cgcgcgaatc cgcgccgttg
      481 acgactttgc cgaccgccga aatcaaaccg ccgtcaagtt ctaaaaacgc catggctttc
      541 ttggcaaccc gcacggccaa tgagtatgcc gatacttcgg ctatcagccg tgcaggcgcg
      601 tctttcagaa attcttcgta cgcggcgaag agatttgaca gcggacgcag cacgagttgg
      661 cgctgattcg ggctaaggtc gtcgaaatcc ttagtccatt gcgcgaccgc ctgcgatgct
      721 tcgcggcagg caaacagtac gctttccccg ttctcggggt cgtccgtgtt gcagtacagg
      781 cccaagcgtt gcacctgttt gacgatatgt tcggcgaagt tgattaactc ctgattgcag
      841 gcgtaacgca tatcctgcag gg
//
LOCUS       DAXFZR010000171          851 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000171 DAXFZR010000000
VERSION     DAXFZR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.172"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..379
                     /locus_tag="ACFJAS_002191"
     CDS             <1..379
                     /locus_tag="ACFJAS_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3983326.1"
                     /translation="LLAEGQKSAVTEYYLNHGEWPANNTSAGVASASEIKGKYVKEVK
                     VEKGVVTATMASSNVNKEIQGKKLSLWAKRENGSVKWFCGQPVKRDDKDNDTVADAAG
                     KDKIDTKHLPSTCRDESSLPGIT"
     gene            418..>851
                     /locus_tag="ACFJAS_002192"
     CDS             418..>851
                     /locus_tag="ACFJAS_002192"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3983327.1"
                     /translation="MASPASNIKGKYVQKVEVNNGVVTATMASSNVNKEIKDKRLSLW
                     AKRQDGSVKWFCGRPVTRDNANAKAGNDDVAKDAGNNGIETKHLPSTCRDESSAVCIE
                     TPPTAFYKNTQTVIPTKAGIRNAKSKETVFPISFCADRSGFPP"
BASE COUNT          263 a          247 c          197 g          144 t
ORIGIN      
        1 ccttttggcc gaaggtcaaa aatcagccgt taccgagtat tacctgaatc acggcgaatg
       61 gcccgcaaac aacacttctg ccggcgtggc atccgcttca gaaatcaaag gcaaatatgt
      121 taaggaagtt aaagtcgaaa aaggcgtcgt taccgccaca atggcttcaa gcaacgtaaa
      181 caaagaaatc caaggcaaaa aactctccct gtgggccaag cgtgaaaacg gttcggtaaa
      241 atggttctgc ggacagccgg ttaagcgcga cgacaaagac aacgacaccg ttgccgacgc
      301 cgccggcaaa gacaaaatcg acaccaagca cctgccgtca acctgccgcg atgaatcatc
      361 gttgccgggt attacctgaa tcacggcata tggccgaaag acaacacttc tgccggcgtg
      421 gcatcccccg cctccaacat caaaggcaaa tatgttcaga aagttgaagt caacaacggc
      481 gtcgttaccg ccacaatggc ttcaagcaac gtaaacaaag aaatcaaaga caaaagactc
      541 tccctgtggg ccaagcgtca agacggttcg gtaaaatggt tctgcggacg gccggttacg
      601 cgcgacaacg ccaacgccaa agccggcaac gacgacgtcg ccaaagacgc cggcaacaac
      661 ggcatcgaaa ccaagcacct gccgtcaacc tgccgcgatg aatcatctgc cgtttgcata
      721 gaaacaccac ctacggcttt ctataaaaat acccaaaccg tcattcccac gaaagcggga
      781 atccggaacg caaaatctaa agaaaccgtt ttcccgataa gtttctgcgc cgatcggtcg
      841 ggattcccgc c
//
LOCUS       DAXFZR010000172          842 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000172 DAXFZR010000000
VERSION     DAXFZR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.173"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            143..496
                     /locus_tag="ACFJAS_002193"
                     /pseudo
     CDS             143..496
                     /locus_tag="ACFJAS_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            494..754
                     /locus_tag="ACFJAS_002194"
     CDS             494..754
                     /locus_tag="ACFJAS_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC3983328.1"
                     /translation="MRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVKIDIRPN
                     GEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
BASE COUNT          289 a          164 c          180 g          209 t
ORIGIN      
        1 atttatctca aataaaatta gagaatgcat acgtgtcttt tgttttgaaa tttatctaac
       61 aaaggaagca caagaatagg tttatagtaa aacatcaaga tgttgaaaat gctgggtttt
      121 aatccaacct acactgaccg gctcagatac agccgctcag cagactctac aaggtattaa
      181 tgatttagga aaattaagtc cggaagcaca acttgctgcc gcgagcctat tacaggacag
      241 tgcctttgcg gtaaaagacg gcatcaattc cgccagacaa tgggctgatg cccatccgaa
      301 tataactgca acagcccaaa ctgcccttgc cgtagcagag gccgcaggta cggtttggcg
      361 cggtaaaaaa gtagaactta acccggccaa atgggattgg gttaaaaata ccggctataa
      421 aacacctgct gttcgccata tgcagactgt agatggggag atggcaggag gaaacaagcc
      481 agttgttaaa tctattagac caactacgcg agatgaatta cgtcaagcat tgcaagaaca
      541 aggttttaga cgtactggtt cagatgcggc tcaatatgaa acatggaaag gtcctgatgg
      601 cgtgaaaata gatattcgtc caaatggaga ggttataaga acccaaagag tgccgcgaac
      661 cgatggtgta cagggaaaat atccgcaacg acaagattat gaaggcaatc cattgccaaa
      721 taatcatcat cattctggat attttgtcaa atgaaaaaaa atatttttca caatgtaagc
      781 ctttatgaaa taatcttttc cgataatgga aataccctta cattatcttt tacagataca
      841 at
//
LOCUS       DAXFZR010000173          821 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000173 DAXFZR010000000
VERSION     DAXFZR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.174"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
BASE COUNT          234 a          173 c          207 g          207 t
ORIGIN      
        1 gatttgaaag gcaacttaga tttgcagctg ttgtttcagg tcgtctgaaa aataaaaagc
       61 aacctgaata acctgttttc cttgcagcaa acctcaaccc caacagccga cccgtcctct
      121 ctccctgtgg gagagagcta gagagagggc aacaagccgc aaggcttgta tttgggcggc
      181 gaaggtatta ggaaagattg ccaccattcg gagaatgccc tccccccagc cctcccccac
      241 gggggaggga gtgggtttcg gcagattttt cggttgccgg tggtttgaaa aataacttaa
      301 atttgcaacc gttgatttca ggtcgtctga aaaagaatgc acgaaatatc aacagcggga
      361 atttttcagg cagcctttat tgtcaagcag atggaacaaa cgccgcgagc gttttttcag
      421 acaatctttg aactcatcgg tagagtgtgt gccgcaaggc gcgcatgcgg tgggtcgggg
      481 ttacagggaa aatgaagaac gcgtgcgtgc gtaccgcaca ccctacatgc gggctacggc
      541 ttgctacgat acgggggttt cgatatacaa gttgggttag caaacccaac attttagaca
      601 attaagcggt ttgtgttagg ttttcaatcc aaactacgct tgccacatga gcgagctatg
      661 acttaccaca cttaaccaag gagttagtaa tgtttagtga tatctacttg aacccaaaaa
      721 actattttgg tatagaaaaa agattgcctt ttaaggcatt ttgggataat ggcggacaat
      781 gctatctaga cttaaaagta gtagatggaa aaataattgc g
//
LOCUS       DAXFZR010000174          810 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000174 DAXFZR010000000
VERSION     DAXFZR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.175"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            72..>810
                     /locus_tag="ACFJAS_002195"
     CDS             72..>810
                     /locus_tag="ACFJAS_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC3983329.1"
                     /translation="MSHHRVLPSAVTEYYPNNGEWPANNGAAGVASSSSIKGKYVKEV
                     EVKNGVVTAQMASSNVNKEIKDKKLSLWAKRQDGSVKWFCGQPVTRANAKADTVADAA
                     DANGIDTKHLPSTCRDKSSAVCTKHLTPISNTFAVAGYCPNHGIWPENNDKAGVASAS
                     KIIGKYVKQVEVKNGVVTATMKSDGVNKEIKDKRLSLWAKRQAGSVKWFCGRPVTRDA
                     AGTDDDAVAKDDTNAIDTKHLPSTCRDE"
BASE COUNT          241 a          246 c          188 g          135 t
ORIGIN      
        1 gcacctgccg tcaacctgcc gtgatacgtc atctgccaaa taaggcaaat taggccttaa
       61 atttcaaata aatcagccat caccgggtat taccctcagc cgtcaccgaa tattacccga
      121 ataacggcga atggcccgca aacaacggcg ctgccggcgt ggcatcttct tcatcaatca
      181 aaggcaaata tgttaaagag gttgaagtta aaaacggcgt cgtcaccgcc caaatggctt
      241 caagcaacgt aaacaaagaa atcaaagaca aaaaactctc cctgtgggcc aagcgtcaag
      301 acggttcggt aaaatggttc tgcggacagc cggttacgcg cgccaacgcc aaagccgaca
      361 ccgttgccga cgccgccgac gccaacggca tcgacaccaa gcacctgccg tcaacctgcc
      421 gcgataaatc atctgccgtt tgcacgaaac acctcacgcc gatttcaaat actttcgccg
      481 ttgccgggta ttgcccgaat cacggcatat ggccggaaaa caacgacaag gccggcgtgg
      541 catccgcttc aaaaatcata ggcaaatatg ttaagcaagt tgaagtcaaa aacggcgtcg
      601 tcaccgccac aatgaaatca gacggcgtaa acaaagaaat caaagacaaa agactctccc
      661 tgtgggccaa gcgtcaagcc ggttcggtaa aatggttctg cggacggccg gttacgcgcg
      721 acgccgccgg caccgacgac gacgccgtcg ccaaagacga caccaacgcc atcgacacca
      781 agcacctgcc gtcaacctgc cgcgatgaac
//
LOCUS       DAXFZR010000175          738 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000175 DAXFZR010000000
VERSION     DAXFZR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.176"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(160..444)
                     /locus_tag="ACFJAS_002196"
     CDS             complement(160..444)
                     /locus_tag="ACFJAS_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC3983330.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWRDLWFDII"
BASE COUNT          197 a          185 c          140 g          216 t
ORIGIN      
        1 aggtctgtcg gcacagaaac ttatcgggta aaacggtttc tttagatttt acgttctaga
       61 ttcccgcctg cgcgggaatg acggtttaga agttgcccga aacctcaaaa aaaaccgaaa
      121 ccgaacaagc cggattcccg cctgagcggg aatgacgggc taaataatat caaaccataa
      181 atcccgccaa gaaacattat tttcttcaat cagttgcaat ttccaagccc tgttccattt
      241 cttcaactgt ttttcccgag taattgcgct ctccatcgta ggatgcagtt cataccaaac
      301 cggcatagta acgttgtacc gtgatgtaaa tccttcaatc aaatgctccc tatgttggta
      361 aatacgttgc accaaatcag atgtaacgcc aatgtataac gtgccattac gttggcttgc
      421 taaaatataa accgcaggct gcatataata cccttttgaa ttatttaaat ttatattccc
      481 gcgaacacca tccggtgatt actttaaccc ttcgttatcc ccatagcttt ccatcattcc
      541 cgcaactctt cgtcattccc gcgcaggcgg gaatccagtg cgttgagttt cagctattta
      601 gaataaattt tgaaactcta atcccatcat tcccacgaaa gtgggaatcc ggttcgttcg
      661 gtttcgcttg ttttaagttt cgggtaactt ccacttcgtc attcccgcgc aggcgggaat
      721 ccagtgcgtt gagtttca
//
LOCUS       DAXFZR010000176          729 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000176 DAXFZR010000000
VERSION     DAXFZR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.177"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(99..383)
                     /locus_tag="ACFJAS_002197"
     CDS             complement(99..383)
                     /locus_tag="ACFJAS_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983331.1"
                     /translation="MAGGNKSLKIGTQSVEKSTGRTIPNNLKEQLAMEEVKANPQGKT
                     PARIPPMSDTKNGWLAKDGWVKRVQNINKVEIHYIENTRTGEKTDFKFKD"
     gene            complement(357..692)
                     /locus_tag="ACFJAS_002198"
                     /pseudo
     CDS             complement(357..692)
                     /locus_tag="ACFJAS_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          175 a          158 c          141 g          255 t
ORIGIN      
        1 aatatgttta tcggcaaaat tggacatata ccattcgtca gtgattggat tggtatttaa
       61 atcatctaaa aaaacattta catcatctaa aaacatggct aatccttaaa cttgaaatct
      121 gttttctcac cggttcttgt attttcaatg taatgtatct caactttgtt tatattttga
      181 acgcgcttaa cccagccgtc ttttgccaac caaccatttt tagtatcgga cataggaggt
      241 attctcgcag gagttttgcc ctgtgggttt gccttaactt cttccattgc taattgttcc
      301 tttaaattat taggtattgt acgaccggtt gatttttcaa cagattgtgt ccctattttt
      361 aatgatttgt ttcccccagc catttccccg tctacagtct gcatatggcg ggcagtaggt
      421 ttttcatagc cggtatcttt aactcaatcc catttagtcg ggttaagttc tactttttta
      481 cctctccaaa ccgtacctgc ggcctctgct acggcaaggg cagtttgggc agtagctgta
      541 atgttcggat gggcatcagc ccattgtctg gcagagttga taccgtcttt taccgcaaaa
      601 gcactgtcct gtaataggct cacggcggca agttgtgctt ccggacttaa ttttcctgaa
      661 ttattaatac cttgtagagt ctgctgcgca gagatgtagc aactgtattt ttaccccgtc
      721 gggtaaaaa
//
LOCUS       DAXFZR010000177          702 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000177 DAXFZR010000000
VERSION     DAXFZR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.178"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(522..>702)
                     /locus_tag="ACFJAS_002199"
     CDS             complement(522..>702)
                     /locus_tag="ACFJAS_002199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983332.1"
                     /translation="FFDFILGISLIHAKIQPNVEKKITANTTLPPLTSNQTDSRFGWK
                     LVYLLFNGECQCSYP"
BASE COUNT          210 a           98 c          171 g          223 t
ORIGIN      
        1 tgcatggtgc atgcattaaa tcagtcttct agcgccgcaa tgggaaaaca tagaagataa
       61 acgaaatgga tgatctaata ctatattttt tatcaggtat attcggaaat caagttgctg
      121 aatatattat aaaaaacaac cgtgagataa aagttccctt tattgcgctt tacgctatat
      181 tttttacgtt gatttatact gtggctttgt tgtttctcag tctgatctat tgggtcaatg
      241 gtgcggagat tgcatggaag gggataggta ttttcagtat gtcggtcagt ttttgtatag
      301 tcttctgtct ttatttggtt gacaaggcag gaagatgtaa ggataagaaa caatagggat
      361 cagatgcggc agcaaacgta gattgggtct aggctcaacc tgcgtttgct gcatctttgg
      421 aagaaatagt ggattcgaaa aaataggaag aattctgatt aatggtatgc gaggtgtagc
      481 agtgggggct gttgctggtg gcataactgg ttatgcaggt tctacgggta agaacactga
      541 cattcgccgt taaataataa ataaaccaac ttccaaccaa acctgctatc ggtttggttg
      601 gaagtgagag gaggtaaggt agtgtttgct gttattttct tttcgacatt gggctgtatt
      661 ttagcatgga taagagatat tcctaaaata aaatcaaaaa aa
//
LOCUS       DAXFZR010000178          675 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000178 DAXFZR010000000
VERSION     DAXFZR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.179"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..492
                     /locus_tag="ACFJAS_002200"
     CDS             <1..492
                     /locus_tag="ACFJAS_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3983333.1"
                     /translation="RKWNDNKYSVNIEKVQEVGKNRRDRKTENQENGSFHAASSLGLS
                     AVYDFRPNDKFKPYIGVRVAYGHVRHGIDSTKKITGTLTVYPNDADAAATVYPDGHPQ
                     KNTYQKSNSSRRLGFGAMAGVGIDVAPGLTLDAGYRYHYWGRLENTRFKTHEASLGVR
                     YRF"
BASE COUNT          186 a          199 c          159 g          131 t
ORIGIN      
        1 agaaagtgga acgacaataa atattccgtc aacatagaaa aggtgcaaga agtcggtaaa
       61 aacaggagag accggaagac ggaaaatcag gaaaacggca gcttccacgc cgcctcctcg
      121 ctcggcttgt ccgccgttta cgatttcaga cccaacgata aattcaaacc ctatatcggt
      181 gtgcgcgtcg cctacggaca cgtcagacac ggcatcgatt cgactaaaaa aataacaggt
      241 actcttaccg tctaccctaa tgatgctgac gcagcagcta cggtttatcc tgacggacat
      301 ccgcaaaaaa acacctatca aaaaagcaac agcagccgcc gcttgggctt cggcgcgatg
      361 gcgggcgtgg gcatagacgt cgcgcccggc ctgaccctgg acgccggcta ccgctaccac
      421 tattggggac gcttggaaaa cacccgcttc aaaacccacg aagcctcatt gggcgtgcgc
      481 taccgcttct gattcgccga ttccgatgcc gtctgaacct tcagacggca tttttaaggc
      541 gcaagatcgg gcaaacggca ttttcagacg gcataactga cagtataatc cgaacatccg
      601 gccgctttcg cgcggccttc agcattatcc gattttttcc gaaagccgaa ccatgcaata
      661 caaacccctc ctgct
//
LOCUS       DAXFZR010000179          667 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000179 DAXFZR010000000
VERSION     DAXFZR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.180"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..324
                     /locus_tag="ACFJAS_002201"
     CDS             <1..324
                     /locus_tag="ACFJAS_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC3983334.1"
                     /translation="EPETLPPAVKKKIMPDGIVYADSPGSRGKLDAGGFTRCRINRSK
                     EFADRRNHINGIGNFWNQAKRALRKYNGIDRKPFPPFLKGWEFRLNFGTPSRQLKILR
                     DRCGI"
     gene            complement(344..505)
                     /locus_tag="ACFJAS_002202"
     CDS             complement(344..505)
                     /locus_tag="ACFJAS_002202"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983335.1"
                     /translation="MALGTSANASNFGISLGKSSAASGTKGIAVGTSSQATNLSAVAI
                     GTESKAQNK"
BASE COUNT          186 a          173 c          137 g          171 t
ORIGIN      
        1 gagcctgaaa cgttgccccc tgccgtcaaa aagaaaatca tgccggacgg tattgtttat
       61 gccgatagcc cgggcagccg cggcaagttg gacgcgggcg gttttacccg ttgccgcatc
      121 aaccgttcca aggaatttgc agaccgtcgg aaccacatta acggcattgg gaacttttgg
      181 aatcaggcaa aacgcgcctt gcgaaaatac aacggaatcg atcgtaaacc ttttccgccg
      241 ttcttgaaag gatgggaatt tcgacttaac ttcggcacac cgtcccggca gctaaaaatc
      301 ctgcgggatc ggtgtggaat ttagggataa tctagtacag cccctattta ttttgtgcct
      361 tactttcagt accaattgcc accgcactta aatttgttgc ctgagaagat gtccccactg
      421 caattccttt cgtaccactg gcagcactgc ttttccctaa ggaaatacca aaattcgatg
      481 cattagcaga agtacccaat gccactcaat tttagatgcc gatgctttat cgttccatga
      541 taaggagcca ttattacctt cttgcaaagt agaatccgcc ccataagaag acaaagcact
      601 cattaacaaa atactagccg ctatcgcgga cgacctatat acataacatt acttcatatc
      661 atttcat
//
LOCUS       DAXFZR010000180          659 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000180 DAXFZR010000000
VERSION     DAXFZR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.181"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            1..353
                     /locus_tag="ACFJAS_002203"
                     /pseudo
     CDS             1..353
                     /locus_tag="ACFJAS_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147169.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
BASE COUNT          185 a          184 c          165 g          125 t
ORIGIN      
        1 aatcatcgtt gccgggtatt gcccgaatca cggcgaatgg ccgaaaaaca acgcttctgc
       61 cggcgtggca tccccccccc accgacatca aaggcaaata tgttaaggaa gttaaagtcg
      121 aaaacggcgt cgttaccgcc caaatgaaac caagcggcgt aaacaatgaa atcaaaggca
      181 aaaaactctc cctgtgggcc aggcgtgaag acggttcggt aaaatggttc tgcggacagc
      241 cggttcagcg caacgccaaa accggcaccg acgccgtcac cgccgacgac aaaaacaaaa
      301 tcgaaaccaa gcacctgccg tcaacctgcc gcgacacttc atctgccgaa taaggcaaat
      361 taggccttaa atttcaaata aatcaaacgg taagtgattt cccacggccg cccggatcaa
      421 cccgggcggc ttgtctttta agggtttgca aggcgggcgg ggtcgtccgt tccgaagcca
      481 tccttttggc cgaaggtcaa aaaaaggtca aaaatcagcc gttgccgggt attgcccgaa
      541 tcacggcata tggccggaaa acttcgtcat tcccgcgaaa gcgggaatct aggtctgtcg
      601 gcacggaaac ttatcgggta aaaaggtttc tccggtcctg agtcctggat tcccacttt
//
LOCUS       DAXFZR010000181          658 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000181 DAXFZR010000000
VERSION     DAXFZR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.182"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(29..>658)
                     /gene="mafB"
                     /locus_tag="ACFJAS_002204"
     CDS             complement(29..>658)
                     /gene="mafB"
                     /locus_tag="ACFJAS_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC3983336.1"
                     /translation="AAARKTLQGIHNLGNLSPEAQLAAATALQDSAFAVKDGINSARQ
                     WADAHPNITATAQTALAVAEAAGTVWGGKKVELNPAKWDWVKNTGYKKPAARHMQTVD
                     GEMAGGNKPLESKNTVTTNNFFENTGYTEKVLRQASNGDYHGFPQSVDAFSENGTVIQ
                     IVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRLFVPNQQLPEK"
BASE COUNT          156 a          149 c          116 g          237 t
ORIGIN      
        1 tttcatttaa catattaatc tctatctact atttttcagg aagttgttgg ttagggacaa
       61 ataatctatg attaattttt ccatctgctt ctctgatata ttcaaaattt ccatcttttc
      121 ctttataaga tccagggata taaagtttat gccttacaat attatctccg ccaactattt
      181 gtattactgt tccattttca gaaaaagcat ctactgattg cggaaaacca tgataatcac
      241 cattcgatgc ttgccttaat actttctctg tataaccggt attttcaaaa aaattattcg
      301 ttgttacagt attttttgat tccagcggtt tattcccccc tgccatctcc ccatctacag
      361 tctgcatatg gcgggcagca ggttttttat agccggtatt tttaacccaa tcccatttgg
      421 ccgggttaag ttctactttt ttacctcccc aaaccgtacc tgcggcctct gctacggcaa
      481 gggcagtttg ggctgttgct gttatattcg gatgggcatc agcccattgt ctggcggaat
      541 taatgccgtc ttttaccgca aaggcactgt cttgtaatgc ggttgcggcc gcaagttgtg
      601 cttccggact taaatttcct aaattgtgaa taccctgtaa agttttccgt gctgccgc
//
LOCUS       DAXFZR010000182          654 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000182 DAXFZR010000000
VERSION     DAXFZR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.183"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
BASE COUNT          154 a          173 c          175 g          152 t
ORIGIN      
        1 ccattcccgc gcaggcggga atccagtccg ttcggtttca gtcatttcca aaaattgccg
       61 tagcgttaag tttctagatt cccactttcg ggggaatgac gcggtgcagg tttccgtgcg
      121 gacggattcg tcattcccac acagacagga atccggattt gtccgcgcgg aaacttatgc
      181 gccgtcattc ccgcacaggc gggaattcag gtctgtcggc ataggaactt atcggataaa
      241 acggttgccc caaccctgcg ttctagattc ccactttcgt gggaatgacg gttcaggtat
      301 tcctaatagc aacccgccac aacccaccta cgcccatcct acgcatctgc catacagccc
      361 tgttccggag cgcgcggata aggtgcgatg ccgccggcgg gcatcatagt cctctctccc
      421 tgtgggagag agctagaaag ctgcaaggct tgtattttag aagactaagg gatttgggaa
      481 agattgtcga aattcaggga gccctctctc ccacggggga gggggcaggt tgcggcagat
      541 ttggcggttg caggcggttt gaaaaacaga ttggatttac aaccgttgat ttcaggtcgt
      601 ctgaaaagca aaaagcagcc tgcacaacct gattagttta caaaaccctt aacc
//
LOCUS       DAXFZR010000183          652 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000183 DAXFZR010000000
VERSION     DAXFZR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.184"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
BASE COUNT          186 a          141 c          173 g          152 t
ORIGIN      
        1 aggacgtaaa atctcaagaa accgttgtac ccgataagtt tccgtgccga caagtccgga
       61 ttcccgcctg cgcgggaatg acggttcagt tgcgtaggac tggattgtga aaaggggcgg
      121 attcggtgaa aacggcggaa atgcgggatt gatggaatcg gcgggctgaa gccctcccta
      181 cgcaacaccc tgcggcgtac tcaaatcaac acggaaactt atgcgccgtc attcccgcgc
      241 aggcgggaat ctagacattc aatgctaagg caatttatcg ggaatgactg aaactcaaaa
      301 aaccggattc ccactttcgt gggaatgacg ggattagagt ttcaaaattt attctaaata
      361 gctgaaactc aacgcactgg attcccgcct gcgcgggaat gacgaagtgg aagttacccg
      421 aaacttaaaa caagcgaaac cgaacgaacc ggattcccac tttcgtggga atgacgggat
      481 tttaggtttc tgattttggt tttctgtttt tgagggaatg atgaaatttt gagttttagg
      541 aatttatcgg aaaaaacaga aaccgctccg ccgtcattcc cgcgcaggcg ggaatctaga
      601 acgtgggaca acagcaatat tcaaaggtta gctgaagctt tagagattct gg
//
LOCUS       DAXFZR010000184          635 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000184 DAXFZR010000000
VERSION     DAXFZR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.185"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(24..>635)
                     /locus_tag="ACFJAS_002205"
     CDS             complement(24..>635)
                     /locus_tag="ACFJAS_002205"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC3983337.1"
                     /translation="ILFYMQYDLAKWAVNARKQADLTQEQLAEAIGFSGKASVSAIEK
                     GRNKPTFDIMLKIAEICHYPLPYQNQIIHNNAEHIQVAGNNFGEVSHVQNFSTENVPI
                     FNLDNISKQHFTKIFSVQAPNNDLQDIGILKDDTLIIDPNLKPKHGNIILVKSIDARG
                     LIAQLSIDLNSRFFLKYNKNEPEMMPNDVNIIGVITNLKRDCI"
BASE COUNT          202 a          102 c          106 g          225 t
ORIGIN      
        1 attgggactt tggcatttgt atgttaaata caatcacgct ttaaattggt aattacacca
       61 ataatattga catcattagg catcatttca ggttcattct tgttgtattt caagaaaaat
      121 ctactgttta aatcaataga taattgggca attaaaccac gagcatcaat agatttaacc
      181 aaaatgatat tgccatgttt gggctttaaa tttgggtcaa tgatgagcgt gtcatctttc
      241 aagataccaa tatcctgcaa atcattatta ggggcttgga ctgaaaagat ttttgtaaaa
      301 tgctgctttg aaatgttgtc taaattgaaa ataggaacat tttcagtaga aaaattttga
      361 acatgagata cttcgccaaa attattgcca gctacttgaa tatgttcagc attattatga
      421 ataatttgat tttgataagg taatgggtaa tgacaaattt ctgcaatttt caacataatg
      481 tcaaaagtag gcttgtttct gcctttttct attgctgata cgctcgcttt tcctgaaaaa
      541 ccaatggctt ccgccaactg ttcttgcgtt aaatctgctt gttttcgggc atttaccgcc
      601 catttcgcta aatcgtattg catataaaac aaaat
//
LOCUS       DAXFZR010000185          624 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000185 DAXFZR010000000
VERSION     DAXFZR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.186"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..452
                     /locus_tag="ACFJAS_002206"
     CDS             <1..452
                     /locus_tag="ACFJAS_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3983338.1"
                     /translation="LEKNRGNRKERKTENQENGTFHAASSLGLSAVYDFKLNDKFKPY
                     IGARVAYGHVRHQVHSMEKETTTVFTVPTGGAPKGGFIPETTISKLPHHESRSISSLG
                     FGAVAGVGIDITPKLTLDAGYRYHNWGRLENTRFKTHEASLGVRYRF"
BASE COUNT          166 a          196 c          149 g          113 t
ORIGIN      
        1 agttggaaaa aaaccggggc aacaggaaag aacggaagac ggaaaatcag gaaaacggta
       61 cgttccacgc cgcctcctcg ctcggcttgt ccgccgttta cgatttcaaa ctcaacgaca
      121 aattcaaacc ctatatcggc gcacgcgtcg cctacggaca cgtcagacat caggttcatt
      181 caatggaaaa agaaaccacg actgttttca ctgtaccaac gggaggcgct ccaaagggag
      241 gttttatccc agaaactacg atcagcaaac ttccccatca cgaaagccgc agcatcagca
      301 gcttgggctt cggcgcagtg gcaggcgtag gcatcgacat cacgcccaag ctgaccctgg
      361 acgccggcta ccgctaccac aactggggac gcttggaaaa cacccgcttc aaaacccacg
      421 aagcctcatt gggcgtgcgc taccgcttct gattccccga ttccgatgcc gtctgaacct
      481 tcagacggca tttttaatcg cccgccgttt acaggcgcgg ggcgggcttg gaaatacccg
      541 aaccgtcatt cccgacaata ccgtaatctc gaaacccgtc cgacaacacc gcaatctcga
      601 aattcgtcat tcccgcgcag gcgg
//
LOCUS       DAXFZR010000186          618 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000186 DAXFZR010000000
VERSION     DAXFZR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.187"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            2..190
                     /locus_tag="ACFJAS_002207"
                     /pseudo
     CDS             2..190
                     /locus_tag="ACFJAS_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(167..>618)
                     /locus_tag="ACFJAS_002208"
     CDS             complement(167..>618)
                     /locus_tag="ACFJAS_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3983339.1"
                     /translation="IKELENKNKNKRDLKTENQENGSFHAVSSLGLSAVYDFKLNDKF
                     KPYIGARVAYGHVRHSIDSTKKTAKILTSSYGNGKPTVYTEENTQNAHRESDSIRRVG
                     LGVIAGVGFDITPKLTLDAGYRYHNWGRLENTRFKTHEASLGVRYRF"
BASE COUNT          115 a          142 c          192 g          169 t
ORIGIN      
        1 aatgacgggt ttcgagatta cggtgttgtc gggaatggcg ggttttaggg ttacggtgta
       61 tcggcaatgg cggtacgggt atttccccgc acctgccccg cgcccgtaaa cggcgggcgc
      121 gtcaaaaatg ccgtctgaag gttcagacgg catcggaatc ggcgaatcag aagcggtagc
      181 gcacgcccaa cgaggcttcg tgggttttga agcgggtgtt ttccaagcgt ccccagttgt
      241 ggtagcggta gccggcgtcc agggtcagct tgggcgtgat gtcgaaaccg acgccggcga
      301 tgacaccgag gcccacgcgg cggatgctgt cgctttcgcg atgggcgttt tgcgtatttt
      361 cctcagtata aaccgtaggt tttccgttac cataggagga ggtaaggatc tttgctgttt
      421 tcttggtcga atcgatgctg tgtctgacgt gtccgtaggc gacgcgcgca ccgatatagg
      481 gtttgaattt gtcgttgagt ttgaaatcgt aaacggctga taagccgaga gaagaaacgg
      541 cgtggaagct gccgttttcc tgattttccg tcttcaggtc tctcttattc ttattcttgt
      601 tttccaactc ttttatgt
//
LOCUS       DAXFZR010000187          595 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000187 DAXFZR010000000
VERSION     DAXFZR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.188"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..518
                     /locus_tag="ACFJAS_002209"
     CDS             <1..518
                     /locus_tag="ACFJAS_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC3983340.1"
                     /translation="FNVAAAAKVAKLAKAAKPGKAAVSGDFSISYKNFSTVKPKVIAK
                     GTINGKTFRDVNQSAKIGSPDSPTLIAQRVNAKIQADGKPRPNATVANSHAEIGVIQQ
                     AYNAGETKGASMTMTVSGKDVCGYCKGDIAAAAQASGLKSLTVNATDNVTGKNKTYYW
                     TPGMKSIKERK"
BASE COUNT          205 a          107 c          131 g          152 t
ORIGIN      
        1 tcttcaacgt tgccgccgcc gccaaagtcg cgaagctggc aaaggcggcg aaaccgggga
       61 aagctgcggt tagcggggat ttttcgatta gctataaaaa cttttctact gtcaaaccaa
      121 aagtaattgc caaaggaacg attaatggaa agacttttag agatgttaat caaagtgcaa
      181 aaattggatc gcctgattca cctacattaa tagcccaacg agttaatgca aaaatccaag
      241 ccgacggtaa acctcgtccg aatgcaaccg ttgctaattc acatgctgaa attggtgtta
      301 tccagcaggc ttataatgca ggtgaaacga aaggggcatc tatgacaatg acagtttcag
      361 gaaaagatgt ttgcggttat tgtaaaggag atattgcagc tgctgctcaa gcttctggac
      421 tgaaatcact gactgttaat gcgacagata atgtaacagg aaaaaataaa acttactatt
      481 ggacgcctgg tatgaaatca attaaggaga gaaaatgaaa acatcaacaa ttgtttttgg
      541 tggatttttt attacagata atggagaacg aatccaaatc cctattttag aaaat
//
LOCUS       DAXFZR010000188          572 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000188 DAXFZR010000000
VERSION     DAXFZR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.189"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(22..267)
                     /locus_tag="ACFJAS_002210"
                     /pseudo
     CDS             complement(22..267)
                     /locus_tag="ACFJAS_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            complement(304..>572)
                     /locus_tag="ACFJAS_002211"
     CDS             complement(304..>572)
                     /locus_tag="ACFJAS_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983341.1"
                     /translation="STIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNE
                     FDEEDGYPVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
BASE COUNT          176 a          113 c           76 g          207 t
ORIGIN      
        1 agaaatctaa ttttaaatta gtcatttgtg tgtaatcttt ccatcaattg atatatttaa
       61 atgcttacca tccctactag cccatccaag ttgctctctt cctgttttag acaattgaac
      121 atcccattca aattcttgac ctttagttct tgatggacct ttagtccatt cattaccaaa
      181 tttatccaaa tatccattat taggtccttt tggtagcggt gctgaaggag agtaattttt
      241 aggtgggata tatctgattc tacctttcta tatcgctgat tctattcatt aagctaatta
      301 tgcttattcc tcaatcacag tctccaccac cgaatgaata cttttaggca attgcaaaaa
      361 agcattccaa gcaattaaag cagttttaaa agtatgcagc gaacagctta atactggata
      421 accatcttct tcatcaaatt cattgtgata aaaaatgacc ttatcatgat gaaattctag
      481 gcaaaattgt tgaccaccgc catcatcata aatttcattt ggattatttt ctattaattc
      541 tataatcttg aaatgctcat caatcgtgga tc
//
LOCUS       DAXFZR010000189          561 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000189 DAXFZR010000000
VERSION     DAXFZR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.190"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
BASE COUNT          117 a          142 c          159 g          143 t
ORIGIN      
        1 tcggttccgt accatccgta ctgcctgcgg cccgccgcct tgtcctgatt tttgttaatc
       61 cgctataaag accgtcgggc atctgcagcc gtcattcccg cgcgggcggg agtctaggcc
      121 ggtcggtgcg gaaacttatc gggaaaaaaa aggtttcttg agattttacg tcctggattc
      181 ccacttttgt gggaatgacg gttcggttgc tacggttact gtcaggtttc ggttatgttg
      241 gaatttcggg aaacttatga atcgtcattc ccgcgcaggc gggaatctag aaatgaaaag
      301 cagcaggaat ttatcggaaa tgaccgaaac tgaacggact ggattcccgc ttttgcggga
      361 atgacggcga cagggttgct gttatagcgg attaacaaaa accggtacgg gttgccccgc
      421 cccggctcaa agggaacgat tctctaaggt gctgaagcac cgggcggatc ggttccgtac
      481 cattcgtact gcctgcggcc cgccgccttg tcctgatttt tgttaatccg ctataaagac
      541 cgtcgggcat ctgcagccgt c
//
LOCUS       DAXFZR010000190          449 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000190 DAXFZR010000000
VERSION     DAXFZR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.191"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..>449
                     /locus_tag="ACFJAS_002212"
     CDS             <1..>449
                     /locus_tag="ACFJAS_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISNgo2 family transposase"
                     /protein_id="HFC3983342.1"
                     /translation="QLEVLKEKIKEQTEKPNCKEGVKRLETIPAIGRMTAAVLFHHLT
                     SSKFETSNKFAAFAGLSPQQKESGTSVRGKGKLTKFGNRKLRAVLFMPAMVAYRIRAF
                     PDFIKRLEEKKKPKKVIIAALMRKLAVIAYHVHKKGGDYDPSRYKSA"
BASE COUNT          154 a           97 c          109 g           89 t
ORIGIN      
        1 aacagcttga agttttaaag gagaagataa aagagcagac ggagaagcct aactgcaagg
       61 aaggcgtgaa gcgtcttgaa accataccgg caataggcag aatgaccgca gccgtattgt
      121 ttcatcatct aacatcttcg aaatttgaaa catcaaacaa atttgcagcg ttcgcaggct
      181 taagcccgca acaaaaagaa tccgggacaa gcgtaagggg aaaaggcaaa ctgaccaagt
      241 ttggcaacag gaaattacgc gccgtcttgt ttatgccggc catggtcgca taccggataa
      301 gggcatttcc cgacttcatc aaaaggctgg aagaaaagaa gaagcctaaa aaagtcatca
      361 tcgcagcatt gatgcgtaaa ctcgccgtta ttgcgtatca cgtacataag aaaggcggag
      421 attacgatcc atcgcgttac aaatcggcg
//
LOCUS       DAXFZR010000191          439 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000191 DAXFZR010000000
VERSION     DAXFZR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.192"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(92..>439)
                     /locus_tag="ACFJAS_002213"
     CDS             complement(92..>439)
                     /locus_tag="ACFJAS_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC3983343.1"
                     /translation="DFDTGSRFKPYAGVRVAYGHVKHQVRSVESETTIVLTTVPGIKV
                     PGRNIQGPIPQPAYHESRSTRRLGFGAMAGVGIDVAPGLTLDAGYRYHNWGRLENTRF
                     KTHEASLGVRYRF"
BASE COUNT           82 a          119 c          143 g           95 t
ORIGIN      
        1 gttcgggtat ttccaagccc gccccgcgcc tgtaaacggc gggcgattaa aaatgccgtc
       61 tgaaggttca gacggcatcg gtatcgggga atcagaagcg gtagcgcacg cccaacgagg
      121 cttcgtgggt tttgaagcgg gtgttttcca ggcgtcccca gttgtggtag cggtacccgg
      181 cgtccagggt caggccgggc gcgacgtcta tgcccacgcc cgccatcgcg ccgaagccca
      241 agcggcgggt gctgcggctt tcgtgataag caggttgggg gatcggacct tgtatgttcc
      301 tgcctggcac tttaatgcca ggcactgtag tgagtacaat cgtggtttcg ctttccactg
      361 aacgaacctg atgtttaacg tgtccgtagg cgacgcgcac gcctgcatag ggtttgaagc
      421 gggaaccggt atcgaaatc
//
LOCUS       DAXFZR010000192          436 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000192 DAXFZR010000000
VERSION     DAXFZR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.193"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(<1..>436)
                     /locus_tag="ACFJAS_002214"
     CDS             complement(<1..>436)
                     /locus_tag="ACFJAS_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983344.1"
                     /translation="YRVISNHWIEPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFK
                     QGEVKELEYVNDNFLPSYLGYFFVALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPV
                     FFLFGYRFYQAKTESGLLLVLISKQEFRTPQSVSSIAVYRIN"
BASE COUNT          175 a           70 c           62 g          129 t
ORIGIN      
        1 ttattaattc gatatacagc aatcgaactt acactttgtg gcgttctaaa ttcctgttta
       61 ctaatcagca ctaataataa ccctgattca gtttttgctt gataaaatcg atagccaaat
      121 aagaaaaaaa cagggttaaa ataaaatgac ttagagcatg aaactagtaa gaaaataatt
      181 ccatacataa caaatagcaa aaaaagatta ttatcgggta tgcttagagc aacaaaaaaa
      241 taaccaagat agctaggtaa gaaattatcg ttaacatatt ctaattcttt cacctctcct
      301 tgcttgaatt catccttccc caagtatttg cttagccata agacagtagc ggttgcgata
      361 aatggtatta gaaaataaaa aagtagactt aacttagaaa taggttctat ccaatgattt
      421 gaaattacac gataaa
//
LOCUS       DAXFZR010000193          399 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000193 DAXFZR010000000
VERSION     DAXFZR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.194"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            complement(112..303)
                     /locus_tag="ACFJAS_002215"
     CDS             complement(112..303)
                     /locus_tag="ACFJAS_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983345.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKITAEIGSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
BASE COUNT          142 a          101 c           55 g          101 t
ORIGIN      
        1 agataaagaa acggcaacac acgccaacag aaaaacatat ttgaacttca tcatattttc
       61 cacataaaag gcagcctgaa aatctttcag gctgcccttg tcaaattatt cctagctttc
      121 ggcttttttg gcaaaccaaa caatccgatt acccgcataa tactttccat ttattgaaat
      181 ccgacaagcc gcgcccaaaa aatgccatgc actgccgatt tccgcagtaa tctttgtacc
      241 gttttcttca aattccaaat attcacccaa taataaactt gaaacagaac gcgtgggcag
      301 caagtgccca ccctacgctt atccaaataa tttgattaaa taaagaataa agaaaatgca
      361 tcaggaacaa aattataatc tgccacctga ctcacaccg
//
LOCUS       DAXFZR010000194          398 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000194 DAXFZR010000000
VERSION     DAXFZR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.195"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
BASE COUNT          125 a           78 c           55 g          140 t
ORIGIN      
        1 tcccttcact gtatttacct gataaatagc ctaaaaagct gcaatcaaat catctatttt
       61 aaaaatttct gtaaaaacag gtaatgcctt taattcatta cctgttttgc cattatttat
      121 ctatgatata agcgtctctt acccttcagc ctctttggga aatcaaagcc tgcttagaac
      181 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
      241 tgtcattgtt ccgtgcaaaa ggaatcttta ttgatgtgtt gtgtactggg tttcagcttg
      301 gctttttaga taatcttttc acaagagcat acatcaatac tgtccaaaaa aaagatttga
      361 taaatatatt gcaacatatt ttattccacc tccatccg
//
LOCUS       DAXFZR010000195          374 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000195 DAXFZR010000000
VERSION     DAXFZR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.196"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
BASE COUNT          114 a           76 c           58 g          126 t
ORIGIN      
        1 aaagagaacg attccctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta
       61 ctgtctgcgg cttcgttgcc ttgtcctgat ttttgttaat ccactatagt ttaaacaact
      121 ttatttttga ttttatccca agtataaacc tgacaatcgt atttaaaagc cggatctacg
      181 cctagcttgt ccactggtat gtatgattta gactaaaaag aaatcaatca tttgctcaat
      241 tttaagcgtc tccccgacag gttcccctgt ctcaggatca aactcaagaa aacctgataa
      301 aaaatcatta ataatcaata atttttcatc actggaaaca gtattgttta taattttttc
      361 ccttagtacg gata
//
LOCUS       DAXFZR010000196          367 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000196 DAXFZR010000000
VERSION     DAXFZR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.197"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..222
                     /locus_tag="ACFJAS_002216"
     CDS             <1..222
                     /locus_tag="ACFJAS_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983346.1"
                     /translation="FNAVPRKEGGVYYYKSCKTCRNKAVRQKRAEKRAAAGAGAMTAA
                     RLHGYIRAAHAACPILGAGLWTQPAGECA"
BASE COUNT           76 a          110 c          132 g           49 t
ORIGIN      
        1 tttaatgccg tcccgcgcaa ggaagggggg gtctattatt acaaatcgtg caaaacctgc
       61 cgcaacaagg cagtccggca aaagcgcgcg gaaaaacgcg cggcggcggg agccggcgcg
      121 atgacggcgg caaggctgca cggatacatc cgcgccgcac acgccgcctg cccgatattg
      181 ggcgccggcc tgtggacgca accggcaggg gaatgcgcgt gacgcgcctt atcctgcctt
      241 accccgtatc ggcaaaccga tattggcgga tttggcgcaa cagggcggtc aggagcgcgg
      301 aggcggcggc gtatagggaa accgtccgcc gtatcgcgca aggggcgggc gcgatgccgt
      361 ccgaagg
//
LOCUS       DAXFZR010000197          360 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000197 DAXFZR010000000
VERSION     DAXFZR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.198"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            280..>360
                     /locus_tag="ACFJAS_002217"
     CDS             280..>360
                     /locus_tag="ACFJAS_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC3983347.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTA"
BASE COUNT          108 a           72 c           86 g           94 t
ORIGIN      
        1 gtggaaaata cagttgctac ggctcgatga atcgtcagaa atacccgaac cgtcattccc
       61 gcgcaggcgg gaatctaggt ctgtcggtgc ggaaatttat cgggtaaaac ggtttcttga
      121 gatttttcgt cctggatccc cactttcgtg ggaatgacgg acggtaaaaa cagggtatga
      181 acacccggtg tctgttatca ctgaatatcc agaatgatta ttattcttgg ggctgtccta
      241 gataactagg gaaattcaaa ttaagttaga attatcccta tgagaaaaag ccgtctaagc
      301 cggtataaac aaaataaact cattgaactg tttgtcgcag gcgtaactgc aagaacagca
//
LOCUS       DAXFZR010000198          352 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000198 DAXFZR010000000
VERSION     DAXFZR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.denovo.199"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
BASE COUNT          121 a           78 c           74 g           79 t
ORIGIN      
        1 tgcggacaat accgtaaccg gcaatgcgga tattacggtc aaacctgccg acccttcaca
       61 gcattcgcgc ccgaaagggc tgattatccg gagcgaccgt aacgaaccaa acaaaaacca
      121 agaagttcgc ttaaataata tagcggatta acaaaaatca ggacaaggcg gcgagccgca
      181 gacagtacaa atagtacggc aaggcgaggc aacgccgtac cggtttaaat ttaatccact
      241 ctatatggct ggtggagttt atttaggcgg aaaaattatt agccctatat atcattcatc
      301 acaagaattt tctggtgaac caataatcta tgctgaaaca aatataattt ta
//
LOCUS       DAXFZR010000199        11300 bp    DNA     linear   BCT 26-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate MA94
            SAMEA113994205-rid23203393.guided.071, whole genome shotgun
            sequence.
ACCESSION   DAXFZR010000199 DAXFZR010000000
VERSION     DAXFZR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA113994205
            Sequence Read Archive: ERR11669512
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002363896.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002363896.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 02:38:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,232
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 112 of 194
            Pseudo Genes (incomplete)         :: 87 of 194
            Pseudo Genes (internal stop)      :: 45 of 194
            Pseudo Genes (multiple problems)  :: 48 of 194
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA113994205-rid23203393.guided.071"
                     /isolate="MA94"
                     /isolation_source="clinical"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="Brazil"
                     /collection_date="2016-01-01"
     gene            <1..1238
                     /gene="trbL"
                     /locus_tag="ACFJAS_002218"
     CDS             <1..1238
                     /gene="trbL"
                     /locus_tag="ACFJAS_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HFC3983348.1"
                     /translation="EQITPSSIVDIGFNLFDKIVENSSIWDPIDSATLIVIGLVILLI
                     LAAVGINMLLMLITTYIVIYSGIIILGFGGGRWTSDMAVGYLKQVLNLSLQLAAMILI
                     IGIGQSIVQDTINTIGTPGFRELAVVLVQTALLVGLVVKIPPMIGSLAGGAGSGGIGS
                     FGVGNAIAAAAMLAGAAAGAGAALKSLAVEAGGLKKAFEAAKAGLGGSNGSSDSGSGK
                     MDSAVDAVAGANAGDKSNQDGKPLTAESSGNSENGSEGSTTGNSGGNPSGSSEENTIS
                     SGSSSSSGEANSTNTSASSGAEGSAEGEATSSETSQSASDSPSNNSGEATQTADTTPK
                     TTNTGGGSPLATAMGGNKVSNWQAAKNVAVGLVSSKWNNAVNNSAGGRLVKHWEDEKK
                     KTEDPTKNPQKNPEGDDNA"
     gene            1231..1803
                     /locus_tag="ACFJAS_002219"
     CDS             1231..1803
                     /locus_tag="ACFJAS_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFC3983349.1"
                     /translation="MLKKTLICILLVGSVHFAMAESLNDALNRSRSGNSNNNRPQVED
                     LFTGDVKLACEATLCLSTGNRPSECQPAIHRYFSIRHKRMGDTIRARHNFLRLCPASK
                     EQGMLELVNAIANGAGRCDARELNRINRSTYIKRVWVGSRRHGDWQEVKVPYIRNAKP
                     SYCSAYFENGWTQLGTRYVGEEKNGGKWVD"
     gene            1803..2402
                     /locus_tag="ACFJAS_002220"
     CDS             1803..2402
                     /locus_tag="ACFJAS_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbN"
                     /protein_id="HFC3983350.1"
                     /translation="MVYVADLPPFMQERVTCSIAAAEKYHLPPHYLLAVAERENGRPG
                     QQVRNTNGTYDVGAMQINTAYIRTLSRYGVTQSAVAQPGCYAYYLAAWRIKRHIVYDR
                     KGDIWTKIANYHSYTPKYNAIYRAAIIRLAEKWERWLAGRQGNNAKGNVPTQAVPAVR
                     TIRSQPLPKQYVSPTTVYVPRTIRVVERSPEPEPETVQN"
     gene            2511..2795
                     /locus_tag="ACFJAS_002221"
     CDS             2511..2795
                     /locus_tag="ACFJAS_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFC3983351.1"
                     /translation="MAYAIAFDLDTTVLQELYPNSSWQNAYGDVKRTLVGLGFNHQQG
                     SVYFGNSEITAVSCVLAAQKLSQTYAWFKPAVSDIRMLRIEEMNDLQPAL"
     gene            complement(2905..3459)
                     /locus_tag="ACFJAS_002222"
     CDS             complement(2905..3459)
                     /locus_tag="ACFJAS_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC3983352.1"
                     /translation="MIIGYLRVSTKEQNVAAQRQVIQDKYQIERWYEDQAASGAVKAL
                     NRPEFRKMAEFVRQGDTVVVVAIDRLGRNTIDVLETVQFLQKKGVAVTSLRENFDLAS
                     PLGTAMLAIVSAFAELERSYIKERQMAGIRNAREEGKKLGRKVKVDPEEIRRWRQSEQ
                     TSISRTAAHFNVSESTVKRACKAK"
     gene            complement(3511..3942)
                     /locus_tag="ACFJAS_002223"
     CDS             complement(3511..3942)
                     /locus_tag="ACFJAS_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC3983353.1"
                     /translation="MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLA
                     DILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVRLSPFLNGCPHDLSLGAESLGKG
                     ESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ"
     gene            complement(3957..5162)
                     /locus_tag="ACFJAS_002224"
     CDS             complement(3957..5162)
                     /locus_tag="ACFJAS_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC3983354.1"
                     /translation="MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQH
                     ADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHNSAAVLSSTQFDEVLKRDEPQKK
                     EVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
                     VSMRAERASDNTYKRFNEYGRGASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQD
                     RNSEFIDKFDDVIKMLSLGSQEQILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESV
                     FAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGIYLLGQAKKAETGQT
                     YSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLE
                     YSAKKSISR"
     gene            complement(5169..5354)
                     /locus_tag="ACFJAS_002225"
     CDS             complement(5169..5354)
                     /locus_tag="ACFJAS_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983355.1"
                     /translation="MNKVEPQESNAIRMIKEACEKNRRMMTDEAFRKEVEKRLYAGPS
                     PELLAKLRVLWAANKEQ"
     gene            complement(5388..6596)
                     /locus_tag="ACFJAS_002226"
     CDS             complement(5388..6596)
                     /locus_tag="ACFJAS_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC3983356.1"
                     /translation="MIEYTPLSAEGKARILNGFMKPRLSRTQSVEQPKIVLVGAQPGA
                     GKSKAASLAKSELRQEGGYIHVDADIMRALIPAPEGVVYSSEQTQKDAGALAISVRNS
                     AKENRRNIVEEGTFRNAASISQFIRDRKSEGYGVEMLAVATASEESVAGIFKRYEEQH
                     AKGVSQPRFVEESYHNEAMAGFKDTLSQCESSFDRVRVTNRAGDILYDSLNRRQNQHE
                     TAKDALSAYQEITPKRLKQVVKAWDEIQLQAESRSIDPIPNYLGMVKQHSEAIYQRVE
                     EIYRQERVVANSEGATLQRKSGDTWQDIEKAEAKGMKAGIHMLGTAKPAKSGREYSGE
                     IVHKDEASVFQKTDQGLIRHKAVQGMAEGKFSSLSEQVEIGQKVSIKREGNELSVKPA
                     DASLKKTMKR"
     gene            complement(6593..6847)
                     /locus_tag="ACFJAS_002227"
     CDS             complement(6593..6847)
                     /locus_tag="ACFJAS_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983357.1"
                     /translation="MQMQETIPGINYEVALELLGDSMRPLIQARESEFVKDKPNQAFI
                     EYCTARLEAIDMLRDSLSPEDTDIIARIFNPDDQTVGRVK"
     gene            complement(7224..7745)
                     /locus_tag="ACFJAS_002228"
     CDS             complement(7224..7745)
                     /locus_tag="ACFJAS_002228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983358.1"
                     /translation="MNMKTVQEYEIIIRLTKENGRYGAAAETVSGGLLESVPPLSAAG
                     NDALYALLHALALKNNFSGCLKEAQRRESVKNIEAKIEEKMKPIELQYYFSDMNVGEF
                     NAYLTVKNLAKPTFISHIDIDEDDKMFIANLAKGIASEYKSLFYLDVNRQTIEKALLR
                     YLEVCVSCNSAQD"
     gene            8133..8249
                     /locus_tag="ACFJAS_002229"
     CDS             8133..8249
                     /locus_tag="ACFJAS_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HFC3983359.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8265..10184
                     /gene="tet(M)"
                     /locus_tag="ACFJAS_002230"
     CDS             8265..10184
                     /gene="tet(M)"
                     /locus_tag="ACFJAS_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFC3983360.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDPVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQKYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10276..10749
                     /locus_tag="ACFJAS_002231"
     CDS             10276..10749
                     /locus_tag="ACFJAS_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC3983361.1"
                     /translation="MPHEQMIDAFSAIIQNSLSLYEEQTPLYYYLTICGSWVLTALGI
                     LSLASVFFTIKQKKPYRFIVPILFLWLGYESIHMPRPNLAVFYGYNLAQLSYPANENP
                     NSFLHLADGSNPICCFIGEDEYLQAKRLGFKEYADAKAKALVDLVEMEKKSKQSD"
     gene            complement(10901..>11300)
                     /locus_tag="ACFJAS_002232"
     CDS             complement(10901..>11300)
                     /locus_tag="ACFJAS_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine modification methylase"
                     /protein_id="HFC3983362.1"
                     /translation="ERMAAGRLVDEIIKIQHNCVSDSRQYGGKLVRIAHEKLLVFKKE
                     IQTALYFLAKAMNRVEAVTAVTWKAAVRRVLQAADGRNMGLTEIYGEMEPYAAARAEN
                     RNWQAKVRQVLQDERFFLRVGKGIYALKTE"
BASE COUNT         3061 a         2615 c         2361 g         3263 t
ORIGIN      
        1 gtgagcagat tacacctagt agtattgttg acataggttt taatttattt gacaaaattg
       61 ttgaaaatag ctcaatctgg gatccaattg atagtgccac tcttattgtt attggtttag
      121 ttattttact aatcttagct gctgtcggaa ttaacatgtt attaatgtta ataacaacct
      181 atattgtcat ctattcagga ataattatct tgggattcgg cggtggacgt tggacatcag
      241 atatggctgt cggatattta aaacaagtat taaacttatc tttacaatta gcagctatga
      301 ttctaattat tggaataggt caatcgattg ttcaagacac tataaacact ataggaaccc
      361 ctggatttag agaactagca gttgttctag ttcaaacagc tttacttgtt ggtttagtcg
      421 ttaaaattcc tccaatgata ggttccctag caggaggagc aggaagtgga ggaattggct
      481 cctttggtgt aggtaatgct attgctgctg ctgctatgtt ggcaggtgct gctgccggag
      541 caggtgcggc attaaaaagc ttagccgtag aagctggtgg tttaaagaaa gcatttgaag
      601 ctgcaaaagc aggcttggga ggttcaaatg gctcatcaga ttcaggtagt gggaagatgg
      661 atagcgcagt tgatgccgtt gcaggtgcaa atgccggaga taaatctaac caagatggaa
      721 aaccactgac tgctgagtcc agtggtaact cagaaaatgg tagtgaagga tctactacag
      781 gaaattcggg aggtaatcca tcaggctcct ccgaagaaaa tacaatttca tccggaagct
      841 cctcaagttc gggggaagca aattcaacaa atacatccgc ttcatcagga gcggaaggta
      901 gtgccgaagg ggaggctact tcatcagaga catctcaatc agcttctgat tcgccaagca
      961 ataatagcgg agaggcgact caaacggctg acaccacacc aaaaacaact aataccggtg
     1021 gaggaagtcc tcttgctact gcgatgggtg gtaataaagt atcaaattgg caggcggcaa
     1081 aaaacgttgc tgtcggcctt gttagcagca aatggaacaa tgccgtcaat aactctgccg
     1141 gtggccgcct agtcaaacac tgggaggacg aaaagaaaaa aacagaagac cccacaaaaa
     1201 atccccaaaa aaaccctgaa ggagatgaca atgcttaaaa aaacgcttat ctgcatattg
     1261 ctggtcggtt ccgtacactt tgctatggcc gaaagcctca acgatgcgct gaaccgcagc
     1321 cgttcgggaa actcgaacaa caacaggccg caggttgaag acttgttcac cggtgatgta
     1381 aagctggcct gcgaagccac gttatgcctg tccacgggca accggccgag tgaatgccaa
     1441 ccggccatcc accgttattt ctctatccgc cacaaacgga tgggagacac aattcgcgcc
     1501 cgacacaact tcctgcgttt atgcccggca tctaaagagc aaggcatgct cgaactggtt
     1561 aatgccattg ccaacggtgc cggtcgatgt gatgcccggg aactgaaccg tatcaaccgt
     1621 tccacttata tcaagcgtgt ttgggtcgga tcaagacggc atggagactg gcaagaggtc
     1681 aaagtcccct atatccgtaa cgccaaacct tcgtactgct ctgcctactt tgaaaacggc
     1741 tggacacagc ttggcacacg ctatgtaggt gaagagaaaa atggcgggaa atgggttgac
     1801 taatggttta tgtggccgac ctgcccccat ttatgcagga acgtgttacc tgctcgattg
     1861 ctgcggctga aaaataccat ctgccgccac attacctgtt ggccgtggcc gaacgcgaaa
     1921 acggtcgtcc cggtcaacag gtacgcaata ccaacggcac ctatgatgta ggcgcgatgc
     1981 agattaatac cgcctatatc cgcaccctat cccgttacgg tgttactcag tcagccgttg
     2041 cccaacccgg atgctatgcc tattacttgg ccgcatggcg gatcaagcgg cacatcgttt
     2101 atgatagaaa gggagatatt tggacaaaga tcgcaaacta ccattcctat acgcctaaat
     2161 acaacgcaat ctatcgcgca gccatcattc ggttggccga aaaatgggaa cggtggcttg
     2221 ccggtaggca agggaataac gccaagggaa atgttcccac acaagctgtt ccagccgttc
     2281 ggacaattcg gtcgcaacca ttgcccaagc aatatgtttc cccgacgacc gtctatgttc
     2341 ctagaacaat ccgtgtggta gagcgttccc cggagccaga gccggaaacc gtccaaaact
     2401 aacagtaaag accatctgaa aaagagtttc agacggtctt ttataatttc cccttgacat
     2461 ggatggtaaa ttcatgttta tatcaattca attatttagg aggtatggaa atggcttacg
     2521 caattgcatt tgatttggat acgaccgttt tacaagaact ttaccctaat agtagctggc
     2581 aaaatgctta cggtgatgta aaaagaacct tggtcggatt aggttttaac catcagcaag
     2641 gcagtgtata ttttggaaac tctgagatta cagccgttag ctgtgtcttg gccgcccaaa
     2701 aactgagcca gacctatgca tggtttaagc cggccgtttc ggatattcgt atgttgcgga
     2761 ttgaggaaat gaacgatttg cagcccgctt tataaagcga taggtatctt agaaaagggc
     2821 agcgcggtgt ccccgccctg ccctttccct ttccttccag cgttgccgag taaaggctgc
     2881 ctagtttcta ctaggcagcc caaactactt tgccttgcaa gcccgtttaa ccgtactttc
     2941 gctaacattg aaatgtgcgg ccgtccggga aatactggtt tgttcagact gccgccatcg
     3001 gcggatttct tcgggatcga ctttcacttt ccgccccagt tttttccctt cttccctagc
     3061 attgcggata ccggccattt gacgctcttt gatgtaactg cgttccaact ccgcaaatgc
     3121 gctgacaata gccagcatcg ccgtacctaa cggactggcc aaatcgaagt tttcccggag
     3181 cgaggttacg gccacgccct ttttctgcaa aaactgcacc gtctccaaaa catcgatggt
     3241 atttctgccc aaacggtcaa tggctactac gactacggta tccccttggc gtacaaattc
     3301 agccatctta cggaactcag gccggttaag ggcttttact gcacccgaag cggcctgatc
     3361 ttcataccac cgttcgatct ggtatttgtc ttgaataacc tgtctttgtg cggccacatt
     3421 ctgctctttg gtcgataccc tcaaatagcc gataatcata attcctcaaa cggattaaaa
     3481 actcttgcgc ccaagtgtga aaaatccttt tcattgcgcg ttaccaccat catatgctcc
     3541 tgtccaacgg tcgtcttcga taagcacacg gtcaaactca atgtctgcaa tatctacctc
     3601 ttccggagga acggccaagg catcagcgat actctccccc ttacccaacg attctgctcc
     3661 taacgaaagg tcatggggac agccattcaa aaacggagac aaccgtacct tataagccgg
     3721 aaaatcggta acaaactttc tcagtttttc atcaaccatc ctttgaacaa atggattgcc
     3781 tgtcgctgcc tcgatgttgt gcaaaatgtc ggccaaatct ccgattatcg tcaaattatc
     3841 cagcgcagcc gcataactct caggccgata cccgcgccgg gaataagaca gttccctgat
     3901 aatcagcgaa atttccgtga tcaaccgcat tttttctttc atttccgtat ccctgcttat
     3961 ctgctgatgg attttttggc ggaatactcc aaagacttga caatcaattt gcccttattg
     4021 cttccgatag aaacgttctg ccctatctca accaatttgg ccagtctttc ttcgttatgt
     4081 gttgcattgt gcctgacaag cccgtttttg gttttttgaa ataccgcagc ggcatccttg
     4141 taaatgattt ctccggaata agtttgacca gtctctgctt ttttagcttg acctagtaaa
     4201 tagataccgg ctttcatgcc tgttacagga atttgttcca ccttagacca caactcgttt
     4261 gctgactttg tttctagggt aacacccttg gagttagtaa ctaccctttg ctgggaatac
     4321 tcttttttgg cgaaaacact ttcaagatcc atactgccct tggcctgatt gaagcattcc
     4381 cggctgattt ttcctgagtc gaagtcgctc tggattttct cggccaacct gcccaatatt
     4441 tgctcttgcg accccaagga aagcattttg atgacatcat cgaacttgtc gataaattca
     4501 ctgttcctgt cttggttgat accgacaatt tttaccgcat caccaaactt atccctgatt
     4561 tgcttgagtc cgtcaggaag attggcctga atatcagcca taatacctat actggctccc
     4621 ctgccgtatt cgttaaaccg tttgtaagta ttgtcggaag ccctttcagc cctcatgctg
     4681 acggctacga tagtaacttc aagattacgc tccagacact gctcaatctt gcgaaaagca
     4741 gactgcggcc gtgcaagctg tccttcaaaa agcagcttgg tggtatcctg catcatcatg
     4801 ttttttactg tcgaagtttt tcccgaaccc ggaattcccg taacgaaaat aacttctttc
     4861 ttctgtggct cgtcgcgttt cagtacctcg tcaaactggg tagaagataa aactgctgct
     4921 gaattgtgga tggcattatt aaccgttgcc ctgtcttctt tgttctcaaa agctggaaac
     4981 aactctttaa acgtatcagc acaaacatat ctaccgcctt ctgtatctgc atgttggcta
     5041 tataaagaca atatctcgtc tgtttgttcg gtcgcaaaac ggatggcctc atcctgaacg
     5101 gaagacaaat attcgttatt gccaaaatct ctcaaattaa tatcgctaga cagttttacc
     5161 atagcggatt attgctcctt atttgccgcc cacaacactc tcaacttagc tagaagctcc
     5221 ggagatgggc cggcatacaa gcgtttttcc acttctttgc ggaaggcttc gtctgtcatc
     5281 atcctgcggt ttttttcgca agcctctttt atcattctga tagcattact ttcttggggc
     5341 tcaactttat tcataataaa atcctaatct taattttata ttactcatca tcgcttcatc
     5401 gttttcttta atgaggcatc tgccggttta acggacaatt catttccttc ccgcttaata
     5461 gaaaccttct gaccaatttc cacctgttcg gacaaactag aaaactttcc ttccgccata
     5521 ccctgaaccg ccttatggcg tatcagacct tggtcagtct tctggaaaac cgatgcctcg
     5581 tctttatgga caatctctcc gctgtattcc ctgccggatt tagccggttt cgccgtgcca
     5641 agcatgtgaa taccggcttt catccccttg gcttcagcct tctcaatatc ctgccatgtg
     5701 tcgcctgatt tgcgttgcag tgttgcccct tctgaattag ccaccaccct ttcctgacgg
     5761 taaatctctt ccacacgctg ataaattgct tcactatgct gtttgaccat gccgagataa
     5821 ttaggtattg gatcgatact gcggctttct gcctgaagct gtatctcgtc ccatgctttt
     5881 acaacctgtt tcaaccgttt aggggttatt tcctgataag ccgatagtgc atcttttgcc
     5941 gtttcatgct ggttttgtcg ccgattcaaa ctgtcataaa ggatgtctcc tgcgcggttg
     6001 gtaacacgaa ccctgtcaaa acttgattca cattgagaca acgtatcctt gaatccagcc
     6061 attgcctcgt tatggtacga ctcttcaaca aatctaggct gtgaaacacc tttggcatgt
     6121 tgttcctcat aacgcttaaa aatcccggct acactctctt cagaagcagt ggctaccgcc
     6181 agcatttcta ccccgtaccc ttctgatttt ctatcacgga taaattggct gatacttgca
     6241 gcatttctaa atgtcccctc ctccacaata ttccgccggt tttcttttgc actattccga
     6301 acactaatgg ccaaagcacc ggcatctttc tgagtttgtt cagacgaata caccaccccc
     6361 tccggcgcag gaatcagtgc acgcataata tctgcatcca catggatata tccgccttct
     6421 tggcgtaact cgcttttagc taaagaagct gccttactct tacccgctcc gggttgtgcc
     6481 ccaactagaa caattttggg ttgctccaca ctttgcgttc tagataaacg tggcttcatg
     6541 aaaccattca gaattcttgc cttaccttcg gcacttaaag gggtgtactc aatcatttta
     6601 cacgtccaac tgtttggtca tccggattga aaatacgcgc gataatatct gtgtcctccg
     6661 gagagagact gtcacgcaac atatcgatag cctcaagcct tgctgtacag tattcgataa
     6721 acgcttggtt tggtttatcc tttacaaact cggactctct tgcctgaatt aatgggcgca
     6781 tcgaatctcc caacaactcc aaagccactt cataattgat accaggaata gtttcttgca
     6841 tttgcatctt ggcttcctcc aaaagttatc cacaaatttt gttagtaagc tgcccatatc
     6901 gccagcctgc cacatataat aaattgaatc ttcgtcatca aaccgttttt gcctgctttt
     6961 taagcaacat ggttcaaaac ctatgtttga ataataagcc agttcaaaat aaataacaac
     7021 gtttttgacc tatacttcca gaccttaacg aaacatggtt cataaagtac actttttgca
     7081 cctacttatc taagttaccc acctcttaat caagatttgg ccattgccca ccatatccca
     7141 taatgtgtag aaaccagtag cgttgggtgg atgacggcgt ttcgggtttt ccgcaggcgg
     7201 caggcagcct gcaatggtag tggctaatcc tgcgcgctat tgcatgagac acaaacttca
     7261 agatagcgca gcaaggcttt ttctatcgtt tgtcgattaa catcaaggta gaagagagac
     7321 ttatattcgg atgcaatgcc ttttgctagg ttggcaataa acatcttgtc gtcttcatct
     7381 atatcaatat ggctgataaa agtcggtttg gccaaattct tgacagtaag ataggcattg
     7441 aattctccaa cattcatatc cgagaaatag tattgcagct ctatcggttt cattttttct
     7501 tcgattttgg cttcaatatt cttcaccgat tcccgtcgtt gtgcttcttt caggcagcct
     7561 gaaaagttgt ttttcagtgc caatgcgtgc agcagagcgt acagggcatc gttaccggct
     7621 gccgacagcg gaggcacgga ttcgagcagt ccgccggaga cggtttcggc tgccgcaccg
     7681 tagcggccgt tctctttcgt caggcggata atgatttcgt attcttggac ggttttcatg
     7741 ttcatactcc cagttgtctt tgttcagtcc gtgctaacgg cgtatccagc cgcactccgg
     7801 cggcggcatc ctgcccgact ctctttcacg tttataatta taggataatt tatcctactt
     7861 gtcaaataaa ttttagccgc taaattttta ctctcgctta tttattcatt ataataaatt
     7921 aatcgttatt ttgtttaact gtaaaattgg agattccttg acaaatatgc tcttacgtgc
     7981 tattatttaa gtgactattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata
     8041 aactgtcaat ttgatagcgg gaacaaataa ttagatgtcc ttttttagga gggcttagtt
     8101 ttttgtaccc agtttaagaa tacctttatc atgtgattct aaagtatcca gagaatatct
     8161 gtatgctttg tatacctatg gttatgcata aaaatcccag tgataaaagt atttatcact
     8221 gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata
     8281 ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata
     8341 acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata
     8401 cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg
     8461 aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat
     8521 atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac
     8581 aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt
     8641 ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag
     8701 agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg
     8761 tgacgaactt taccgaatct gaacaatggg atatggtaat agaaggaaat gattaccttt
     8821 tggagaaata tacgtctggg aaattattgg aagcattaga actcgaacaa gaggaaagca
     8881 taagatttca taattgttcc ctgttccctg tttatcacgg aagtgcaaaa aacaatatag
     8941 ggattgataa ccttatagaa gtgattacga ataaatttta ttcatcaaca catcgaggtc
     9001 agtctgaact ttgcggaaaa gttttcaaaa ttgagtattc ggaaaaaaga cagcgtcttg
     9061 catatatacg tctttatagt ggcgtactgc atttgcgaga tccggttaga atatcggaaa
     9121 aggaaaaaat aaaaattaca gaaatgtata cttcaataaa tggtgaatta tgtaaaatcg
     9181 ataaggctta ttccggggaa attgttattt tgcagaatga gtttttgaag ttaaatagtg
     9241 ttcttggaga tacaaagcta ttgccacaga gagagagaat tgaaaatccc ctccctctgc
     9301 tgcaaacgac tgttgaaccg agcaaacctc aacaaaggga aatgttactt gatgcacttt
     9361 tagaaatctc cgacagtgac ccgcttctgc gatattatgt ggattctgcg acacatgaaa
     9421 tcatactttc tttcttaggg aaagtacaaa tggaagtgac ttgtgctctg ctgcaagaaa
     9481 agtatcatgt ggagatagaa ataaaagagc ctacagtcat ttatatggaa agaccgttaa
     9541 aaaaagcaga gtataccatt cacatcgaag ttccaccgaa tcctttctgg gcttccattg
     9601 gtctatctgt agcaccgctt ccattaggga gcggagtaca gtatgagagc tcggtttctc
     9661 ttggatactt aaatcaatcg tttcaaaatg cagttatgga ggggatacgc tatggctgtg
     9721 aacaaggatt gtatggttgg aatgtgacgg actgtaaaat ctgttttaag tatggcttat
     9781 actatagccc tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac
     9841 aagtcttaaa aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg
     9901 cgccacagga atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg
     9961 tagacactca attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta
    10021 ttcaaaaata tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag
    10081 agttaaaagg gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata
    10141 gtcggataga taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt
    10201 atataaatat ggtttcttgt taaataagat gaaatatttt ttaataaaaa ttaactgtga
    10261 gtgattggag acaccatgcc acatgagcaa atgattgatg ctttttcagc aatcattcaa
    10321 aactccttaa gcctatatga agagcaaact ccgctatact actacctgac tatctgcggt
    10381 tcttgggttc tgactgcact cggcatcctc tccttggcgt ccgtattctt taccatcaag
    10441 cagaaaaaac cataccgttt tatcgtcccg attctgtttt tatggctggg atatgaaagc
    10501 atccacatgc cgcgcccgaa tcttgctgta ttctacggat acaacttggc gcagctttcc
    10561 tatccggcaa acgaaaatcc caactcgttc ctacatctcg cagacggctc aaatccgatt
    10621 tgctgcttca taggcgaaga cgagtatttg caagccaaac ggttaggttt caaagagtat
    10681 gccgatgcaa aagcaaaggc tttggttgat ttggttgaaa tggagaaaaa atccaaacaa
    10741 tctgattgaa tcttaaattc aataataacg cggtaggggg ctggtcaagc gaaggttcag
    10801 gctctgactg aaatttcgct tggacagggg gtgtggggcg gagcgcagcg aagggctacc
    10861 gcgtggcggc cgaagccgcc cgacaaactc cagtttgtcg ctattccgtt ttcagtgcgt
    10921 aaataccttt ccccactctc agaaaaaacc gctcgtcctg taatacctgc ctgactttgg
    10981 cctgccagtt cctgttttct gcccgcgcag cggcatacgg ttccatttcg ccataaattt
    11041 ccgtcaagcc catattcctc ccgtctgccg cctgcaatac ccgtcttacg gcggctttcc
    11101 atgtaacggc cgttaccgcc tcaaccctat tcatcgcttt agccaaaaag tacaaggccg
    11161 tctgaatctc ttttttaaag accagcagtt tctcatgggc aatccgtacc agctttccgc
    11221 catattggcg gctgtcgctg acgcagttgt gctggatttt gataatctca tccaccagcc
    11281 gccccgccgc catccgttca
//
